㸦 ࢣࢿ࣑◂✪⏍࿤⛁Ꮥ㡷ᇡỞỞ⏍࿤ࢨࢪࢷ࣑᝗ሒ⤣ྙࢸ࣭ ࢰ࣊ ࣭ࢪࡡᵋ⠇࡛ࢣࢿ࣑᝗ሒ⌦Ꮥࡡ๭ᠺ
Biological Systems Database and Genome Information Scienceࣈࣞࢩ࢘ࢠࢹ࣭ࣛࢱ࣭㔘஁ ᐹ  ா㒌ኬᏕ໩Ꮥ◂✪ᡜ࣬ᩅ᤭㸦㸣◂✪┘Ⓩ ࢣࢿ࣑ࡡ᝗ሒࡢࠉ⣵⬂࣬಴మ࣬⏍∸⏲࡛࠷ࡖࡒ㧏ḗࣝ࣊ࣜࡡ⏍࿤ࢨࢪࢷ࣑ࢅཋ⌦Ⓩ࡞⌦よࡌࡾࡒࡴࡡᇱ┑᝗ሒ࡚࠵ࡽࠉྜྷ᫤࡞༈⒢ࡷ⏐ᴏࢅࡢࡋࡴ࡛ࡊࡒᚺ⏕ࡡྊ⬗ᛮࢅࡵࡗ᝗ሒ࡚ࡵ࠵ࡾࠊࡊ࠾ࡊ࡝࠿ࡼࡆ
ࡿࡱ࡚ࡡ᝗ሒᢇ⾙࡚ࡢࠉࢣࢿ࣑࡞᭡࠾ࡿࡒ಴ࠍࡡ㐿ఎᏄࡷࢰࣤࣂࢠ㈻ࢅよㄖࡌࡾࡆ࡛ࡢ࡚ࡀ࡙ࡵࠉࡆࡿࡼᇱᮇ㒂ဗ࠾ࡼᵋᠺࡈࡿࡾ⏍࿤ࢨࢪࢷ࣑࡛ࡊ࡙ࡡࡢࡒࡼࡀࢅ├ࡔ࡞よㄖࡌࡾࡆ࡛ࡢ࡚ࡀ࡝࠾ࡖࡒࠊࡐࡆ࡚ᮇ◂✪࡚ࡢࠉࢣࢿ࣑࠾ࡼ⣵⬂࡚ࣝ࣊ࣜࡡ⏍࿤ࢨࢪࢷ࣑ࡡࡢࡒࡼࡀ࡛᭯⏕ᛮࢅぜ࠷ࡓࡌࡒࡴࡡ▩ㆉ㞗⣑ᆵࢸ࣭ࢰ࣭࣊ࢪ㸝⏍࿤ࢨࢪࢷ࣑᝗ሒ⤣ྙࢸ࣭ࢰ࣭࣊
ࢪ KEGG㸞ࢅᵋ⠇ࡊࠉ⏍࿤ࢨࢪࢷ࣑ࡡ᝗ሒᵋ⠇ཋ⌦ࢅ⌦よࡌࡾ࡛ྜྷ᫤࡞ࠉ⏐ᴏ໩࡫ࡗ࡝࠿ࡾࣁ࢕࢛࢕ࣤ
ࣆ࢚࣏ࢷ࢔ࢠࢪᢇ⾙ࢅ☔❟ࡌࡾࠊ

㸧-㸧㸣KEGG ࢸ࣭ࢰ࣭࣊ࢪࡡᵋ⠇ KEGG ࡢ᪟ᩝ㒂┤ࣃࢹࢣࢿ࣑ࣈࣞࢡ࣑ࣚ➠Ϩ᭿ࡡ᭩⤂ᖳᗐ࡚࠵ࡾ 1995 ᖳ࡞≁ᏽ㡷ᇡ◂✪ࠔࢣࢿ࣑᝗ሒࠕࡡୖ࡚㛜ጙࡊࠉ➠ϩ᭿࡞≁ᏽ㡷ᇡ◂✪ࠔࢣࢿ࣑ࢦ࢕࢙ࣤࢪࠕ࡚Ⓠᒈࡈࡎ࡙ࡀࡒࢸ࣭ࢰ࣭࣊ࢪ࡚࠵ࡾࠊ࣐
ࣝࢼ࢓࣑ࣈࣞࢩ࢘ࢠࢹࡡୌ㈇࡛ࡊ࡙ 2000 ᖳ࡞㛜ጙࡈࡿࡒᮇ◂✪࡞࠽ࡄࡾ᩺ࡊ࠷ KEGG ࡢࠉ⣵⬂࡚ࣝ࣊ࣜࡡ⏍࿤ࢨࢪࢷ࣑ࡡࢤࣤࣅ࣭ࣖࢰ⾪⌟࡚࠵ࡽࠉୖᅒ࡞♟ࡊࡒࡻ࠹࡞ࠉࢣࢿ࣑᝗ሒ㸝GENES㸞࡛ࢢ࣐࢜ࣜ᝗
ሒ㸝LIGAND㸞ࢅࢾࢴࢹ࣭࣠ࢠ᝗ሒ㸝PATHWAY㸞࡚⤣ྙࡊࡒࠔ⏍࿤ࢨࢪࢷ࣑᝗ሒ⤣ྙࢸ࣭ࢰ࣭࣊ࢪࠕ
࡚࠵ࡾ [2, 3]ࠊ㸧㸣◂✪ᠺᯕᴣさ㸧-㸦㸣᩺ࡊ࠷ࢸ࣭ࢰ࣭࣊ࢪࡡᴣᛍ ࣁ࢕࢛࢕ࣤࣆ࢚࣏ࢷ࢔ࢠࢪࡡ✪ᴗࡡ┘ᵾࡢࠉ⣵⬂࣬಴మ࣬⏍∸⏲࡛࠷ࡖࡒ⏍࿤ࢨࢪࢷ࣑ࢅࢤࣤࣅ࣭ࣖࢰࡡ୯࡞්⌟ࡊࠉ㧏ḗ々㞟ࢨࢪࢷ࣑࡛ࡊ࡙ࡡ⏍࿤⌟㇗ࢅࢣࢿ࣑ࡡ᝗ሒ࠾ࡼゝ⟤࡚஢ῼྊ⬗࡞ࡌࡾࡆ࡛
࡚࠵ࡾ [1]ࠊࡆࡡࡻ࠹࡝ふⅤ࠾ࡼࠉᮇ◂✪࡚ࡢࠔ⏍࿤ࢨࢪࢷ࣑ࡡࢤࣤࣅ࣭ࣖࢰ⾪⌟ࠕ࡛࠷࠹ࢸ࣭ࢰ࣭࣊ࢪࡡ᩺ࡊ࠷ᴣᛍࢅᥞ၌ࡊࠉࡆࡿࢅᐁ⏕໩ࡊࡒࠊ ᚉᮮࡡࣁ࢕࢛࢕ࣤࣆ࢚࣏ࢷ࢔ࢠࢪᢇ⾙࠿ࠉኬ㔖ࢸ࣭ࢰࡡࢪࢠ࣭ࣛࢼࣤࢡ࡚᭯⏕㐿ఎᏄࡷ᭯⏕ࢰࣤࣂࢠ㈻࡝࡜಴ࠍࡡ㒂ဗࢅぜ࠷ࡓࡌࡆ࡛࡞୹╉࠿࠵ࡖࡒࡡ࡞ᑊࡊࠉᮇ◂✪࡚ࡢ㒂ဗ㛣ࡡ㒼⥲ᅒ㸝┞பష⏕ࢾࢴࢹ࣭࣠ࢠ㸞ࢅ᪺ࡼ࠾࡞ࡌࡾࡆ࡛࡚ࠉ⏍మࢨࢪࢷ࣑ධమ࡛ࡊ࡙ࡡᶭ⬗ࡷ᭯⏕ᛮࢅぜ࠷ࡓࡌ᪁Ἢㄵࢅ㛜ᢽࡊ
ࡒࠊKEGG ࡞㞗⣑ࡈࡿࡒ㒼⥲ᅒࡡ▩ㆉࡢࠉ௥ㅨ්ᵋ⠇ࢅࡢࡋࡴࠉࢣࢿ࣑ࡡ᝗ሒ࠾ࡼ⣵⬂࡚ࣝ࣊ࣜࡡ⏍࿤ࢨࢪࢷ࣑ࡡᶭ⬗よㄖࡡࡒࡴࠉᅗ㝷Ⓩ࡞ᖕᗀࡂฺ⏕ࡈࡿ࡙࠷ࡾࠊ
 KEGG ࢸ࣭ࢰ࣭࣊ࢪᵋ⠇࡞㛭ࡊ࡙ࠉᮇ◂✪ࡡරమⓏ࡝ᠺᯕࡢ௧ୖࡡ㏳ࡽ࡚࠵ࡾࠊ㸝ᩒ⌦␊ྒ㸯⏍㸢1㸢8㸞

(1) ࢾࢴࢹ࣭࣠ࢠ㸝ࣂࢪࢗ࢘࢕㸞᝗ሒ࡞㛭ࡊ࡙ࡢࠉ௥
ㅨ⣌୯ᚨࡡ KEGG ࠾ࡼࢨࢡࢻࣜఎ㐡ࢅࡢࡋࡴ࡛ࡊࡒ
ᵕࠍ࡝โᚒ⣌ࡷ⑋Ẵࡡࣂࢪࢗ࢘࢕ࢅྱࡳ KEGG ࡫࡛Ⓠᒈࡈࡎࡒࠊࡱࡒ KEGG ࣂࢪࢗ࢘࢕࣏ࢴࣈࡡ XML໩ࢅᐁ⌟ࡊࠉࣂࢪࢗ࢘࢕ࢸ࣭ࢰ࣭࣊ࢪࡡᅗ㝷ᵾ‵࡛࡝ࡖࡒࠊ(2) ࢣࢿ࣑᝗ሒ࡞㛭ࡊ࡙ࡢࠉKEGG ࣂࢪࢗ࢘࢕࡞ᑊᚺࡘࡄ࡙࢛࣭ࢮࣞࢡ㐿ఎᏄࢡ࣭ࣜࣈ KO ࢅᏽ⩇ࡊࠉࢣࢿ࣑୯ࡡ㐿ఎᏄ࡞ KO ࡘࡄࢅ⾔࠹ࡆ࡛࡚ࠉࣂࢪࢗ࢘࢕්ᵋ⠇࡛ࡐࡿ࡞ఔ࠹㧏ḗᶭ⬗よ㔐ࢅྊ⬗࡛ࡊࡒࠊKO ࡘࡄࡡ⮤ິ໩ࢨࢪࢷ࣑ࢅᗀࡂᥞ౩ࡌࡾࡆ࡛࡚ࠉ㐿ఎᏄ࢓ࢿࢷ࣭ࢨࣘࣤ࡞࠽࠷࡙ࡵ KEGG ࡢᅗ㝷ᵾ‵࡞࡝ࡾ࡛⩻࠻࡙࠷ࡾࠊ
(3) ࢢ࣐࢜ࣜ᝗ሒ࡞㛭ࡊ࡙ࡢࠉ໩ྙ∸࣬໩Ꮥཬᚺ࡞ຊ࠻࡙⢶㙈ࡡᵋ㏸᝗ሒࢅࢸ࣭ࢰ࣭࣊ࢪ໩ࡊࡒ [4]ࠊࡱࡒ
໩Ꮥᵋ㏸Ẓ㍉࢓ࣜࢥࣛࢫ࣑ࢅ㛜Ⓠࡊ࡙ࠉ໩ྙ∸ࡷ⢶㙈ࡡ㢦జᵋ㏸᳠⣬ࢅྊ⬗࡛ࡊࠉࡈࡼ࡞⏍మහ໩Ꮥཬᚺࡡฦ㢦మ⣌ RC ࢅ㛜Ⓠࡊ࡙ࠉEC ␊ྒࡘࡄࡡ⮤ິ໩ࢅᐁ⌟ࡊࡒ [5, 6]ࠊࡆࡡࡻ࠹࡝඙㥉Ⓩ◂✪࡞ࡻࡽࠉ⡷ᅗ NCBIࠉḚᕗ EBIࠉ⡷ᅗ⢶㙈ࢤࣤࢮ࣭ࢨ࢓࣑࡝࡜࡛ࡡᅗ㝷㏻ᦘ࠿㐅ࢆ࡚࠷ࡾࠊ(4) ᖕᗀ࠷ᇱ┑Ⓩ࡝ࢸ࣭ࢰ࣭࣊ࢪ࡚࠵ࡾ KEGG ࢅ಴ࠍࡡࢼ࣭ࢫ࡞ᚺࡋฺ࡙⏕࡚ࡀࡾࡻ࠹ࠉᵾ‵Ⓩ࡝ࣈ
ࣞࢡ࣐ࣚࣤࢡ࢕ࣤࢰ࣭ࣆ࣭࢘ࢪ KEGG API ࢅ㛜Ⓠࡊᥞ౩ࡊࡒࠊ㸧-㸨㸣ࢣࢿ࣑᝗ሒ⌦Ꮥࡡ๭ᠺ ᮇ◂✪࡚ࡢࠉ⏍࿤ࢨࢪࢷ࣑ࡡࢤࣤࣅ࣭ࣖࢰ⾪⌟㸝࢛ࣤࢹࣞࢩ࣭㸞࡞ࡗ࠷࡙ࠉࢾࢪࢷࢴࢺࢡࣚࣆ㸝㝭ᒒࢡࣚࣆ㸞࡛ࣚ࢕ࣤࢡࣚࣆࡡᴣᛍࢅᑙථࡊࡒࠊࢾࢪࢷࢴ
ࢺࢡࣚࣆࡢ KEGG ࣂࢪࢗ࢘࢕ࡡ㝭ᒒ⾪⌟࡞౐ࢂࡿࠉࢣࢿ࣑࠾ࡼࣂࢪࢗ࢘࢕්ᵋ⠇࡛㧏ḗᶭ⬗よ㔐ࢅ⾔࠹᪁Ἢㄵ࡛ࡊ࡙ᐁ⏕໩ࡊࡒࠊ ࡵ࠹㸦ࡗࡡᴣᛍ࡚࠵ࡾࣚ࢕ࣤࢡࣚࣆ࡛ࡢࢿ࣭ࢺ࡛
࢙ࢴࢩࢅථࡿ᭨࠻ࡒࢡࣚࣆࡡࡆ࡛࡚ࠉ௥ㅨ⣌࡞࠽࠷࡙㓕⣪㸝㐿ఎᏄ㸞ࢾࢴࢹ࣭࣠ࢠ࡛໩ྙ∸ࢾࢴࢹ࣭࣠ࢠࡡ┞⿭ᛮ࡞㛭ࡌࡾᴣᛍ࡚࠵ࡾࠊࡆࡿࢅࡵ࡛࡞ࢣࢿ࣑୯ࡡ㐿ఎᏄࣝࣂ࣭ࢹ࣭ࣛ࠾ࡼ⏍∸࠿⏍⏐ࡊᚋࡾ∸㈻ࢅ஢ῼࡊࡒࡽࠉ㏣࡞ኮ↓∸ࡡᵋ㏸࠾ࡼࢣࢿ࣑୯ࡡ
㐿ఎᏄࡷྙᠺ⤊㊪ࢅ஢ῼࡊࡒࡽࠉࢣࢿ࣑࡛ࢢ࣐ࢪࢹ࣭ࣛࢅ⼝ྙࡊࡒ◂✪ࢅ㛜ᢽࡊࡒ [4-6]ࠊࡆࡿࡢ㎾ᖳࡡࢢ࣐࢜ࣜࢣࢿ࣐ࢠࢪ◂✪࡛࡛ࡵ࡞ࠉࢣࢿ࣑࡛⎌ሾ࡛ࡡ┞பష⏕ࢅ⌦よࡌࡾࢣࢿ࣑᝗ሒ⌦Ꮥࡡ◂✪㡷ᇡ࡛ࡊ࡙ࡈࡼ࡞Ⓠᒈࡊࡗࡗ࠵ࡾࠊ㸨㸣⤎ㄵ KEGG ࢅ୯ᚨ࡛ࡊࡒࢣࢿ࣑ࢾࢴࢹࡡࢗ࢘ࣇࢦ࢕ࢹ㸝http://www.genome.jp/㸞࡫ࡡ࢓ࢠࢬࢪ௲ᩐࡢࠉᮇ◂✪㛜ጙ᫤࡞᭮㛣 200 ୒௲࡚࠵ࡖࡒࡡ࠿⤂஡᫤࡞ࡢ᭮
㛣 800 ୒௲࡞㐡ࡊࠉ㸪ᖳ㛣࡚ KEGG ࠿㣍㌅Ⓩ࡞Ⓠᒈࡊࡒࡆ࡛ࢅ∸ㄊࡖ࡙࠷ࡾࠊ࢓ࢠࢬࢪ௲ᩐࡡ㸫ࠤ㸭๪ࡢᾇአ࠾ࡼ࡚࠵ࡽࠉᅗ㝷Ⓩ࡝▩Ⓩ᝗ሒᇱ┑࡛ࡊ࡙ࡡᆀనࢅ☔❟ࡊࡒࠊࡆࡿࡢୖ࡞♟ࡊࡒ Google ࡡࣛࣤࢠ᳠⣬㸝௙ࡡࢦ࢕ࢹ࠾ࡼ࡜ࡡ⛤ᗐࣛࣤࢠࡈࡿ࡙࠷ࡾ࠾ࡡ┘Ꮽ㸞࡚ࡵ᪺ࡼ࠾࡚࠵ࡽࠉKEGG ࡢୠ⏲࡚᭩ࡵࡻࡂฺ⏕ࡈࡿ࡙࠷ࡾࢸ࣭ࢰ࣭࣊ࢪࡡ㸦ࡗ࡛࡝ࡖࡒࠊ

㸩㸣୹࡝Ⓠ⾪ㄵᩝ
1. Kanehisa, M. and Bork, P. (2003) Bioinformatics inthe post-sequence era. Nat. Genet. 33, 305-310.
2. Kanehisa, M., Goto, S., Kawashima, S., and Nakaya,A. (2002) The KEGG databases at GenomeNet.
Nucleic Acids Res. 30, 42-46.
3. Kanehisa, M., Goto, S., Kawashima, S., Okuno, Y.,and Hattori, M. (2004) The KEGG resource fordeciphering the genome. Nucleic Acids Res. 32,
D277-D280.
4. Hashimoto, K., Goto, S., Kawano, S., Aoki-Kinoshita,
K.F., Ueda, N., Hamajima, M., Kawasaki, T., andKanehisa, M. (2005) KEGG as a glycome informatics
resource. Glycobiology, in press.
5. Hattori, M., Okuno, Y., Goto, S., and Kanehisa, M.
(2003) Development of a chemical structurecomparison method for integrated analysis of chemicaland genomic information in the metabolic pathways. J.
Am. Chem. Soc. 125, 11853-11865.
6. Kotera, M., Okuno, Y., Hattori, M., Goto, S., andKanehisa, M. (2004) Computational assignment of theEC numbers for genomic-scale analysis of enzymatic
reactions. J. Am. Chem. Soc. 126, 16487-16498.

㸝ᩒ⌦␊ྒ㸯⏍㸢1㸢8㸞