2015 China-Japan-Korea Symposium
on Analytical Chemistry

Program and Abstracts

October 13 – 15, 2015
Haeundae Grand Hotel, Busan, Korea

2015 China-Japan-Korea Symposium
on Analytical Chemistry

October 13 – 15, 2015
Haeundae Grand Hotel, Busan, Korea

Organized by
Korea Food Research Institute
The Korean Analytical Science Research Group
China-Japan-Korea Analytical Science Discussion Group

Supported by
Dong-Il Shimadzu Corp. KOREA
The Korean Society of Analytical Science and Technology
The Japan Society for Analytical Chemistry
Rigong Int’l Inc., KOREA
Shimadzu Corp., JAPANShimadzu Corp., CHINA
Hwashin Instrument Co. LTD. KOREA
LECO Korea Co. LTD.
Seoul Women’s University
Kangwon National University

Message from Chairman
You are cordially invited to participate in the 2015 China-Japan-Korea Symposium on
Analytical Chemistry (CJK 2015), to be held in Haeundae Grand Hotel, Busan, Korea on
October13-15, 2015. The Conference should provide a forum for the analytical scientists
to discuss their recent progress, to exchange the ideas and experiences on a broad range ofanalytical sciences, and to promote international cooperation and friendship.
A number of fruitful discussions and suggestions are expected during the conference.
Outstanding analytical scientists from Korea, China and Japan are to be invited to present
plenary and keynote lectures on a broad range of topics for the opening of China-JapanKorea Symposium on Analytical Chemistry.
In this opportunity the organization committee appreciates to Dr. Yong-Kon Park, thepresident of Korea Food Research Institute, and several sponsors, Professor Jin-Ming Lin
of China and Professor Hiroshi Sato of Japan delegate for their all consideration for this
symposium. We are pleased to have you join us to share your very recent research ideas
and activities, and extended our friendship. We are looking forward to welcoming you tobeautiful Busan in the autumn of 2015.

Professor Jaeho HA, Ph.D.
Chair of the Organizing Committee of 2015 CJK Symposium.

１

ORGANIZING COMMITTEE
Chairperson : Dr. Jaeho Ha
Food Analysis Center
Korea Food Research Institute1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam, Gyeonggi, 463-746, Korea
jhkfri@kfri.re.kr, Tel: +82-31-780-9127, Fax: +82-31-780-9280
Korea University of Science and Technology
217 Gajeong-ro Yuseong-gu, Daejeon, Korea
Co-Chair: Prof. Jin-Ming Lin
Tsinghua University, CHINA
Co-Chair: Prof. Hiroshi Sato
Nagasaki International University, JAPAN２

COMMITTEE MEMBERS

KOREA
Prof. Man-Goo Kim
Kangwon National University
Prof. Sunyoung Bae
Seoul Women’s University
Prof. Seong Ho Kang
Kyung Hee University
Prof. Joon Myong Song
Seoul National University
Dr. Jang-Hyuk Ahn
KOTITI Testing & Research InstituteProf. Yong-Moon Lee
Chungbuk National University
Dr. Haewon Jang
Korea Food Research Institute
Mr. Dongwon Seo
Korea Food Research Institute
Dr. Tae-Gyu Nam
Korea Food Research Institute
Ms. Jooyoung Lee
Korea Food Research Institute
Ms. Jisu Park
Korea Food Research Institute３

CHINA

Prof. Xiurong Yang
Chinese Academy of Sciences
Prof. Jin-Ming Lin
Tsinghua University
Prof. Jian-Hua Wang
Northeastern University
Prof. Xi Chen
Xiamen University
Dr. Yuki Hashi
Shimadzu (China) Co., Ltd.
Prof. Zilin Chen
Wuhan University
Prof. Huwei Liu
Peking University
Prof. Chunhua Ma
Wuyi University
Prof. Shunxing Li
Minnan Normal University
Prof. Gongke Li
Sun Yat-sen University
Prof. Yi Chen
Chinese Academy of Sciences
Prof. Bi-Feng Liu
Huazhong University

４

JAPANProf. Hiroshi Sato
Nagasaki International University
Dr. Tsuneaki Maeda
Advanced Industrial Science and Technology
Prof. Daniel Citterio
Keio University
Prof. Kazuaki Ito
Kinki University
Prof. Dai Kato
Advanced Industrial Science and Technology (AIST)
Prof. Katsumi Uchiyama
Tokyo Metropolitan University
Prof. Kei Toda
Kumamoto University
Prof. Kazuichi Hayakawa
Kanazawa University５Lecture Rooms Floor Plan

６

2015 CJK SCIENTIFIC PROGRAMMETUESDAY, OCTOBER 13th, Hotel Lobby
13:30 - 18:00

Registration

WEDNESDAY, OCTOBER 14th, SKY HallOPENING CEREMONY
08:00 - 08:30

Opening Ceremony
Prof. Jaeho Ha, Korea Food Research Institute, Korea

Session 1, PLENARY LECTURES
08:30 - 09:00
09:00 - 09:30

Chairperson : Prof. Seong Ho Kang

Study of DNA/Small Molecule Interaction by Dual Polarization Interferometry
Prof. Xiurong Yang, Chinese Academy of Sciences, China
Inkjet-printed analytical devices made from paper
Prof. Daniel Citterio, Keio University, Japan
Determination of trans fatty acids in daily consumed foods using a silver ion

09:30 - 10:00

cartridge solid phase extraction method coupled with GCXGC-TOFMS
Prof. Jaeho Ha, Korea Food Research Institute, Korea

10:00 - 10:30

Generation of Droplets of Liquid for Mass Spectrometry
Prof. Jin-Ming Lin, Tsinghua University, China

10:20 - 10:50

Coffee BreakSession 2, KEYNOTE LECTURES
10:50 - 11:10

Chairperson : Prof. Joon Myong SongGold-containing Nanocarriers/Nanocomposites for Anti-bacterial Applications
Prof. Jian-Hua Wang, Northeastern University, China
Simultaneous and selective determination of inorganic nitrogen species in

11:10 - 11:30

seawater samples
Prof. Kazuaki Ito, Kinki University, Japan
Odor variation on the car air conditioning evaporator according to the feeding

11:30 - 11:50

nutrients
Prof. Man-Goo Kim, Kangwon National University, Koreath

11:50 - 13:00

Luncheon, EMERALD Hall, 6 Floor

Session 3, KEYNOTE LECTURES
13:00 - 13:20

Chairperson : Prof. Jian-Hua -Wang

Development of on-line SFE-SFC system and its application
Dr. Yuki Hashi, Shimadzu (China) Co., Ltd., China
Quantitative on-line Concentration and Reaction for Capillary Electrophoresis

13:20 - 13:40

with Inkjet Sample Introduction
Prof. Katsumi Uchiyama, Tokyo Metropolitan University, Japan

13:40 - 14:00

Introduction of 2016 CJK Symposium in China
Prof. Chunhua Ma, Wuyi University, China
Detection of Benzo[a]pyrene-induced Breast Cancer Stem Cells Using

14:00 - 14:20

Quantum dot-based High-content Assay
Prof. Joon Myong Song, Seoul National University, Korea

７

14:20 - 14:40

Coffee BreakSession 4, KEYNOTE LECTURES

Chairperson : Prof. Jang-Hyuk Ahn

Micro ion extractor for transferring inorganic and organic trace anions from a
14:40 - 15:00

single drop of whole blood
Prof. Kei Toda, Kumamoto University, JapanThe research of sample preparation method based on metal organic

15:00 - 15:20

frameworks
Prof. Gongke Li, Sun Yat-sen University, China
LC-MS/MS analysis of sphingolipid metabolites in mice during acute exercise

15:20 - 15:40

stressProf. Yong-Moon Lee, Chungbuk National University, Korea

15:40 - 16:00

Microfluidic Chips towards Cell Signaling Analysis
Prof. Bi-Feng Liu, Huazhong University, China

16:00 - 17:00

Poster Presentations

Session 5, KEYNOTE LECTURES & ORAL PRESENTATIONS
Chairperson : Prof. Yi Chen

17:00 - 17:20

Determination of Important Volatile Compounds from Aqueous Matrix
Prof. Sunyoung Bae, Seoul Women’s University, Korea

17:20 - 17:35

Electrochemical study on electron transfer across DNA base pairs
Prof. Jifeng Liu, Tianjin University, China
Antioxidant Activity of Volatile Compounds Isolated from Broccoli (Brassica

17:35 - 17:50

oleracea L.) sprouts by GC and GC-MS
Dr. Hae Won Jang, Korea Food Research Institute, Korea

17:50 - 18:05

Analytical Applications of Higher-order Calibration Methods Based on
Alternating Multilinear Decomposition Algorithms
Prof. Hai-Long Wu, Hunan University, Chinath

18:30 - 21:00

Banquet, EMERALD Hall, 6 Floor

WEDNESDAY, OCTOBER 14th, PRIVATE Hall
Session 1, KEYNOTE LECTURES

Chairperson : Prof. Shin-Ichi Ohira

The synthesis of fluorescent graphitic carbon nitride nanomaterials and their
10:50 - 11:10

application in fluorescence sensing
Prof. Xi Chen, Xiamen University, China
Sputtered nanocarbon film electrodes for bioelectroanalysis

11:10 - 11:30

Prof. Dai Kato, National Institute of Advanced Industrial Science andTechnology (AIST), Japan
Break diffraction-limited barrier with wavelength-modulation fluorescence-free

11:30 - 11:50

nanoscopy
Prof. Seong Ho Kang, Kyunghee University, Korea

11:50 - 13:00

thLuncheon, EMERALD Hall, 6 Floor

Session 2, KEYNOTE LECTURES

Chairperson : Prof. Xi Chen

８

Novel Solid Phase Microextraction and Capillary Electrochromatographic
13:00 - 13:20

Column for Pharmceutical Analysis
Prof. Zilin Chen, Wuhan University, China
Discrimination technique using fragmentless ionization mass spectrometry and13:20 - 13:40

multivariate analysis for food analysis
Prof. Takahisa Tsugoshi, National Institute of Advanced Industrial Science andTechnology (AIST), Japan

13:40 - 14:00
14:00 - 14:20

Fast Analysis by Microextraction Coupled to Ambient Mass Spectrometry
Prof. Huwei Liu, Peking University, China
Determination of copper content in processed food by ICP
Dr. Jang-Hyuk Ahn, KOTITI Testing & Research Institute, Korea

14:20 - 14:40

Coffee BreakSession 3, KEYNOTE LECTURES

Chairperson : Prof. Hailong Wu

Lysine surface modified Fe3O4@SiO2@TiO2 microspheres-based
14:40 - 15:00

preconcentration and photocatalysis for in situ selective determination of
nanomolar dissolved organic and inorganic phosphorus in seawater
Prof. Shunxing Li, Minnan Normal University, China
Electrochemical and optical monitoring of enzyme reaction on compact disc-

15:00 - 15:20

type microchip
Prof. Toshihiko Imato, Kyushu University, Japan

15:20 - 15:40

Strategy toward the ultrafast separation
Prof. Yi Chen, Chinese Academy of Sciences, China
Polycyclic Aromatic Hydrocarbons, Nitropolycyclic Aromatic Hydro- carbons and15:40 - 16:00

Inorganic Ions in Snow Layers at Murodo, Tateyama, Japan
Prof. Kazuichi Hayakawa, Kanazawa University, Japan

16:00 - 17:00

Poster Presentations

Session 4, ORAL PRESENTATIONS
17:00 - 17:15

Chairperson : Prof. Shunxing Li

Application of novel nanomaterials in the phosphopeptide enrichment
Prof. Yu Bai, Peking University, China
Water Quality Monitoring of Drainage River in Kuantan, Malaysia by using Ion-

17:15 - 17:30

exclusion/Ion-exchange Chromatography
Dr. Daisuke Kosaki, University Malaysia Pahang , Japan
Preparation and Application of Microextraction Probe for Enrichment of

17:30 - 17:45

Organics from Aqueous Matrices
Dr. Hai-fang Li, Tsinghua University, China

18:30 - 21:00

thBanquet, EMERALD Hall, 6 Floor

THURSDAY, OCTOBER 15th, PRIVATE Hall
Session 1, ORAL PRESENTATIONS
09:00 - 09:15
09:15 - 09:30

Chairperson : Yu Bai

Multifunctional fluorescent optical sensors for bioanalysis
Prof. Yanqing Tian, South University, China
Facile preparation of a novel benyl imidazolium ionic liquids hybrid monolithic
column for residue analysis of nitrogen-heterocyclic pesticides by CEC

９Prof. Xiaoping Wu, Fuzhou University, China
09:30 - 09:45

Electrodialytic Matrix Isolation and Preconcentration for Ionic Solutes
Prof. Shin-Ichi Ohira, Kumamoto University, Japan
Electrothermal vaporization or atomization on tungsten coil for analytical atomic

09:45 - 10:00

spectrometry
Prof. Xiandeng Hou, Sichuan University, China
Determination of silicate, borate and carbonate ions in natural waters using ion-

10:00 - 10:15

exclusion chromatography coupled with charged aerosol detector
Prof. Nobutake Nakatani, Rakuno Gakuen University, Japan

10:30 – 11:00

Closing Ceremony (Young Scientists & Best Posters Awards)

THURSDAY, OCTOBER 15th, CITY Room
Session 1, ORAL PRESENTATIONS
09:00 - 09:15

Chairperson : Prof. Kei Toda

Development of a Hybrid Pyrolyzer for Gas Chromatography
Prof. Naoki Oguri, Japan Analytical Industry Co., Ltd., Japan
New strategies for the functionalization of polymer monolith and applications to

09:15 - 09:30

separation and preconcentration of organic compounds
Prof. Qiong Jia, Jilin University, China

09:30 - 09:45
09:45 - 10:00
10:00 - 10:15
10:30 – 11:00

Determination of arsenic species in food by LC-ICP-MS
Dr. Cheong-Tae Kim, NONGSHIM CO., LTD., Korea
Functionalized Gold-Nanomaterials Fluorescence Probe for Cyanide Detection
Prof. Shaomin Shuang, Shanxi University, China
Hairpin DNA Template-based Copper Nanoparticles for the Detection of NAD+
Prof. Jianzhong Lu, Fudan University, China
Closing Ceremony (Young Scientists & Best Posters Awards), PRIVATE Hall

１０

List of Participants from Korea
Name

Organization

E-mailJaeho Ha, Dr.

Korea Food Research Institutejhkfri@kfri.re.kr

Haewon Jang, Dr.

Korea Food Research Institutehwjkfri@kfri.re.kr

Jinbong Hwang, Dr.

Korea Food Research Institutehwangjb@kfri.re.kr

Dongwon Seo, Mr.

Korea Food Research Institutedwseo@kfri.re.kr

Tae-Gyu Nam, Dr.

Korea Food Research Institutentg97@kfri.re.kr

Jooyoung Lee, Ms.

Korea Food Research Institutejylee@kfri.re.kr

Jisu Park, Ms.

Korea Food Research Institutegoo819@kfri.re.kr

Seong Ho Kang, Prof.

Kyunghee University

shkang@khu.ac.kr

Moonhee Park, Ms.

Kyunghee University

moonhee@khu.ac.kr

Sujin An, Ms.

Kyunghee University

tnwls8210276@naver.com

ChangHee Kang, Prof.

Jeju National University

changhee@jejunu.ac.kr

Jung-Min Song, Dr.

Jeju National University

songjm@jejunu.ac.kr

Jun-Oh Bu, Mr.

Jeju National University

uaeveros@jejunu.ac.kr

YongMoon Lee, Prof.

Chungbuk National University

ymleefn@hanmail.net

HwaMi Lee, Dr.

Gyeongin Regional Food and Drug

lhm9904@naver.com

Administration
JaeYoung Shim, Dr.

Busan Regional Korea Food and Drugshilvi0321@gmail.com

Administration
Sunyoung Bae, Prof.

Seoul Women's University

sbae@swu.ac.kr

Soomi Park, Ms.

Seoul Women's University

aclsoom2@naver.com

Soyoung Lee, Ms.

Seoul Women's University

kitty_sy@naver.com

Yura Hwang, Ms.

Seoul Women's University

yura4931@naver.com

Dong-Sun Lee, Prof.

Seoul Women's University

dslee@swu.ac.kr

Joon Myong Song, Prof. Seoul National University

jmsong@snu.ac.kr

Jieun Ki, Ms.

Seoul National University

jieunki@snu.ac.kr

Gee Woo Nam, Ms.

Seoul National University

nameraser@snu.ac.kr

Seoul National University

baskaran@snu.ac.kr

Hee Ju, Ms.

Seoul National University

juhee9079@snu.ac.kr

Jang-Hyuk Ahn, Dr.

KOTITI

ahn5470@hanmail.net

Cheong-Tae Kim, Dr.

NONGSIM CO., LTDkctmass@hanmail.net

Man-Goo Kim, Prof.

Kangwon National University

mgkim@kangwon.ac.kr

Yunje Kim, Prof.

Korea Institute of Science and Technology

yjkim@kist.re.kr

Ho Soo Lim, Dr.

Food and Drug Safety

hslim70@korea.kr

Yunsuk Kim, Dr.

K-water Instituteyunskim@kwater.or.kr

Wonjin Yoon, Mr.

Korea Food Research InstituteYoon.Won-jin@kfri.re.kr

Baskaran
Purushothaman

１１

Name

Organization

Haejin Wang, Ms.

Korea Food Research InstituteSarah15232477@gmail.com

Jangho Lee, Mr.

Korea Food Research Institutepenguini@daum.net

So Young Kim, Ms

Korea Food Research Institute07419@kfri.re.kr

Eun-Joo Park, Ms

Korea Food Research Institute07727@ kfri.re.kr

Ahmad Rois Mansur, Mr. Korea Food Research Institute１２E-mailrais.ahmad07@yahoo.com

List of Participants from China
Name

Organization

E-mailXiurong Yang, Prof.

Chinese Academy of Sciences

xryang@ciac.ac.cn

Jin-Ming Lin, Prof.

Tsinghua University

jmlin@mail.tsinghua.edu.cn

Jian-Hua Wang, Prof.

Northeastern University

jianhuajrz@mail.neu.edu.cn

Xi Chen, Prof.

Xiamen University

xichen@xmu.edu.cn

Yuki Hashi, Dr.

Shimadzu Chinay-hashi@shimadzu.co.jp

Zilin Chen, Prof.

Wuhan University

chenzl@whu.edu.cn

Chunhua Ma, Prof.

Wuyi University

chma@wuyiu.edu.cn

Huwei Liu, Prof.

Peking University

hwliu@pku.edu.cn

Gongke Li, Prof.

Sun Yat-sen University

cesgkl@mail.sysu.edu.cn

Yi Chen. Prof.

Chinese Academy of Sciences

chenyi@iccas.ac.cn

Bi-Feng Liu, Prof.

Huazhong University

bfliu@mail.hust.edu.cn

Shunxing Li, Prof.

Minnan Normal University

lishunxing@mnnu.edu.cn

Yu Bai, Prof.

Peking University

yu.bai@pku.edu.cn

Hai-Fang Li, Prof.

Tsinghua University

lihaifang@mail.tsinghua.edu.cn

Yanqing Tian, Prof.

South University

tianyq@sustc.edu.cn

Shaomin Shuang, Prof.

Shanxi University

smshuang@sxu.edu.cn

Jifeng Liu, Prof.

Tianjin University

jfliu@tust.edu.cn

Hai-Long Wu, Prof.

Hunan University

hlwu@hnu.cn

Xiaoping Wu, Prof.

Fuzhou University

wapple@fzu.edu.cn

Jianzhong Lu, Prof.

Fudan University

jzlu@shmu.edu.cn

Qingxiang Wang, Prof.
Qiong Jia, Prof.
Zhuo Deng, Ms.
Mingcong Rong, Mr.
Ting-Ting Zhao, Ms.
Ming-Li Chen, Prof.
Xu-Wei Chen, Prof.
Ting Yang, Prof.
Fengming Chen, Dr.
Zhaochai Qiu, MS.
Feng Luo, Prof.

Fujian Normal University
Jilin University
Xiamen University
Xiamen University
Xiamen Huaxia University
Northeastern University
Northeastern University
Northeastern University
Tsinghua University
Xiamen University
Fujian Research Instituteaxiang236@126.com
jiaqiong@jlu.edu.cn
joydeng2011@163.com
haiyangzhixinrmc@qq.com
ztt@hxxy.edu.cn
chenml@mail.neu.edu.cn
chenxuwei@mail.neu.edu.cn
yangting@mail.neu.edu.cn
chenfengmingok@mail.tsinghua.edu.cn
308466917@qq.com
luofeng6789@163.com

Kailai Xu, Dr.

Sichuan University

xukailai@scu.edu.cn

Guo-Jun Zhang, Dr.

Hubei University

gjzhangtj@hotmail.com

Shin-Ichi Kawano, Dr.

Shimadzu China

kawashin@shimadzu.co.jp

Hong-Kai Wang, Mr.

Shimadzu China

bswhk@shimadzu.com.cn

Dongtao Lu, Prof.

Shanxi University

ludongtao@sxu.edu.cn

１３

Name

Organization

E-mailErqun Song, Prof.

Southwest University

eqsong@swu.edu.cn

Mohamed Foda, Prof.

Huazhong Agricultural University

m.frahat@fagr.bu.edu.eg

Xiandeng Hou, Prof.

Sichuan University

houxd@scu.edu.cn

Xue Jiang, Ph.D. student

Sichuan University

jiangxue218@gmail.com

Chenghui Li, Ph.D. student Sichuan University

lch_lucy@163.com

Lan Wu, Prof.

wulan@scu.edu.cn

Sichuan University

１４

List of Participants from Japan
Name

Organization

Toshiyuki Hobo, Prof.

Tokyo Metropolitan University

toshi-90@nifty.com

Kazuaki Ito, Prof.

Kinki University

itok@hiro.kindai.ac.jp

Dai Kato, Dr.
Tsuneaki Maeda, Dr.

Advanced Industrial Science andTechnology
Advanced Industrial Science andTechnology

E-maildai.kato@aist.go.jp
maeda-t@aist.go.jp

Hiroshi Sato, Prof.

Nagasaki International University

satoh@niu.ac.jp

Katsumi Uchiyama, Prof.

Tokyo Metropolitan University

uchiyama-katsumi@c.metro-u.ac.jp

Hitachi high-tech Sciencemasaki.watanabe.yk@hitachi-

Corporation

hightech.com

Shin-Ichi Ohira, Prof.

Kumamoto University

ohira@sci.kumamoto-u.ac.jp

Kei Toda, Prof.

Kumamoto University

todakei@sci.kumamoto-u.ac.jp

Toshihiko Imato, Prof.

Kyushu University

imato@cstf.kyushu-u.ac.jp

Masaki Watanabe, Dr.

Takahisa Tsugoshi, Prof.

Advanced Industrial Science andTechnology

tsugoshi.takahisa@aist.go.jp

Kazuichi Hayakawa, Prof.

Kanazawa University

hayakawa@p.kanazawa-u.ac.jp

Naoki Oguri, Dr.

Japan Analytical Industry Co. Ltd.

n-oguri@jai.co.jp

Daisuke Kosaki, Dr.

University Malaysia Pahangemerald.green.2-10@hotmail.co.jp

Nobutake Nakatani, Prof.

Rakuno Gakuen University

nakatani@rakuno.ac.jp

Daniel Citterio, Prof.

Keio University

citterio@applc.keio.ac.jp

Ryozo Goto, Dr.

goto2000@yahoo.co.jp

１５

List of Participants from Mongolia
Name

Organization
Scientific food research andGanjuurjav Oyun, Dr.

manufacturing SAMO InstituteDondogsuren Narantuya, Ms.
Enkhbat Anudari, Ms.

Scientific food research andmanufacturing SAMO InstituteScientific food research andmanufacturing SAMO Institute１６E-mailganjuuro@yahoo.com
narantuya0620@gmail.com

List of Sponsors
Dong-Il Shimadzu Corp. KOREA
The Korean Society of Analytical Science and Technology
The Japan Society for Analytical Chemistry
The Japan Society for Analytical Chemistry
Rigong Int’l Inc., KOREA
Shimadzu Corp., JAPANShimadzu Corp., CHINA
Hwashin Instrument Co. LTD. KOREA
LECO Korea Co. LTD.

１７

PLENARY LECTURES
PL.01
Study of DNA/Small Molecule Interaction by Dual Polarization Interferometry
Xiurong Yang*, State Key Laboratory of Electroanalytical Chemistry, ChangchunInstitute of Applied Chemistry, Chinese Academy of Sciences, China. E-mail: xryang@ciac.ac.cn
PL.02
Inkjet-printed analytical devices made from paper
Daniel Citterio* and Koji Suzuki, Department of Applied Chemistry, Keio University, 314-1 Hiyoshi, Kohoku-ku, 223-8522 Yokohama, Japan. E-mail: citterio@applc.keio.ac.jp
PL.03
Determination of trans fatty acids in daily consumed foods using a silver ion cartridge
solid phase extraction method coupled with GCxGC-TOFMS
Dongwon Seo1, Jaeho Ha1,2*, 1Food Analysis Center, Korea Food Research Institute,2
Korea University of Science and Technology. E-mail: jhkfri@gmail.com

PL.04
Generation of Droplets of Liquid for Mass Spectrometry
Jin-Ming Lin1*, Chen Luo1, Yangdong Zhang1, Fengming Chen1,2, Katsumi Uchiyama2,
1
Department of Chemistry, Tsinghua University, Beijing 100084, China, 2Department ofApplied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo
Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0364, Japan. E-mail:jmlin@mail.tsinghua.edu.cn
KEYNOTE LECTURES
KL.01
Gold-containing Nanocarriers/Nanocomposites for Anti-bacterial Applications
Bo Hu, Ning Wang, Ming-Li Chen, Xu-Wei Chen, Jian-Hua Wang*, Research Center for
Analytical Sciences, Northeastern University, Shenyang 110819, China. Email:jianhuajrz@mail.neu.edu.cn
KL.02
The synthesis of fluorescent graphitic carbon nitride nanomaterials and their application influorescence sensing
１８

Xi Chen1,2*, Mingcong Rong1, Yiru Wang1, 1Department of Chemistry and the MOE Key
Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry andChemical Engineering, Xiamen University, Xiamen 361005, China, 2State Key Laboratoryof Marine Environmental Science, Xiamen University, Xiamen 361005, China. Email:xichen@xmu.edu.cn
KL.03
Simultaneous and selective determination of inorganic nitrogen species in seawater
samplesKazuaki Ito*, Department of Biotechnology and Chemistry, Faculty of Engineering,
Kinki University, 1 Umenobe, Takaya, Higashi-Hiroshima, 739-2116, Japan. Email:itok@hiro.kindai.ac.jp
KL.04
Sputtered nanocarbon film electrodes for bioelectroanalysis
Dai Kato* and Osamu Niwa, National Institute of Advanced Industrial Science andTechnology (AIST), Higashi, Tsukuba, Ibaraki 305-8566, Japan. Email:dai.kato@aist.go.jp
KL.05
Odor variation on the car air conditioning evaporator according to the feeding nutrients
Man-Goo Kim*, Young-Jae Ryu, Dept. of Environmental Science, College of Natural
Science, Kangwon National University, Kangwon, 200-701, Korea Email:mgkim@kangwon.ac.kr
KL.06
Break diffraction-limited barrier with wavelength-modulation fluorescence-freenanoscopy
Peng Zhanga, Seungah Leeb, Seong Ho Kanga,b,*, aDepartment of Chemistry, Graduate
School, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea,b
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446701, Republic of Korea. Email: shkang@khu.ac.kr
KL.07
Development of on-line SFE-SFC system and its application
Yuki Hashi, Shimadzu (China) Co., Ltd., China. Email: y-hashi@shimadzu.co.jp
KL.08
Novel Solid-Phase Microextraction and Capillary Electrochromatographic Column
１９Techniques for Pharmaceutical Analysis
Zilin Chen*, Wenpeng Zhang, Juan Zhang, Zixin Zhang, School of Pharmaceutical
Sciences, Wuhan University, China. Email: Chenzl@whu.edu.cn
KL.09
Quantitative on-line Concentration and Reaction for Capillary Electrophoresis with Inkjet
Sample Introduction
Ying Rang, Fengming Chen, Hulie Zeng, Hizuru Nakajima, Shungo Kato, Jinmig Lin,
Katsumi Uchiyama*, Department of Applied Chemistry, Graduate School of Urban
Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo
192-0397, Japan. Email: uchiyama-katsumi@tmu.ac.jp
KL.10
Discrimination technique using fragmentless ionization mass spectrometry andmultivariate analysis for food analysis.
Takahisa Tsugoshi*, National Institute of Advanced Industrial Science and Technology(AIST), Japan. E-mail: tsugoshi.takahisa@aist.go.jp
KL.11
Introduction of 2016 CJK Symposium in China
Chunhua Ma*, College of Tea and Food Science, Wuyi University, Wuyi Rd. 16,
Wuyishan 354300, China. E-mail: chma@wuyiu.edu.cn
KL.12
Fast Analysis by Microextraction Coupled to Ambient Mass Spectrometry
Xin Wang, Xianjiang Li, Yu Bai, Huwei Liu*, Institute of Analytical Chemistry, College
of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. Email: hwliu@pku.edu.cn
KL.13
Detection of Benzo[a]pyrene-induced Breast Cancer Stem Cells Using Quantum Dotbased High-content Assay
Yumi Shim, Joon Myong Song*, College of Pharmacy, Seoul National University, Seoul
151-742, Korea. E-mail: jmsong@snu.ac.kr
KL.14
Determination of copper content in processed food by ICPJae Sung Kim, Yoon Jung Yoo, Byeong Hyeon So, Kwang Sik Jung, Hyun Mi Koo, Ji
Won Lee, Jang-Hyuk Ahn*, Sang Rag Lee, Young Ryul Kim, KOTITI Testing &２０

Research Institute, Seongnam 462-807 Republic of Korea. E-mail: ahn5470@hanmail.net
KL.15
Micro ion extractor for transferring inorganic and organic trace anions from a single dropof whole blood
Kei Toda*, Hiroka Nishiyama, Yukihide Nakamura, Yurika Imoto, Shin-Ichi Ohira
Department of Chemistry, Kumamoto University, 2-39-1, Kurokami, Kumamoto 860-8555,
Japan. E-mail: todakei@sci.kumamoto-u.ac.jp
KL.16
Lysine surface modified Fe3O4@SiO2@TiO2 microspheres-based preconcentration andphotocatalysis for in situ selective determination of nanomolar dissolved organic andinorganic phosphorus in seawater
Shunxing Li*, Wenjie Liang, College of Chemistry and Environment & Fujian Province
Key Laboratory of Modern Analytical Science and Separation Technology, Minnan
Normal University, Zhangzhou 363000, P. R. China. E-mail: lishunxing@fjzs.edu.cn
KL.17
The research of sample preparation method based on metal organic frameworks
Gongke Li*, Yuling Hu, Zelin Huang, Haixian Lian, Yichun Huang,Suling Zhang, Yifeng
Fan, Zhuomin Zhang, School of Chemistry and Chemical Engineering, Sun Yat-sen
University, Guangzhou, 510275, P.R.China. E-mail: cesgkl@mail.sysu.edu.cn
KL.18
Electrochemical and optical monitoring of enzyme reaction on compact disc-type
microchip
Toshihiko Imato*, Departament of Applied Chemistry, Graduate School of Engineering,
Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395 Japan. E-mail:imato@cstf.kyushu-u.ac.jp
KL.19
LC-MS/MS analysis of sphingolipid metabolites in mice during acute exercise stressYong-Moon Lee*, College of Pharmacy, Chungbuk National University, Cheongju 361763, Korea. E-mail: ymleefn@chungbuk.ac.kr
KL.20
Strategy toward the ultrafast separation
Yi Chen*, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. .
E-mail: chenyi@iccas.ac.cn
２１KL.21
Microfluidic Chips towards Cell Signaling Analysis
Pen Chen, Yao Wang, Bi-Feng Liu*, College of Life Science and Technology, Huazhong
University of Science and Technology, Wuhan 430074, E-mail: China,
bfliu@mail.hust.edu.cn
KL.22
Polycyclic Aromatic Hydrocarbons, Nitropolycyclic Aromatic Hydro- carbons andInorganic Ions in Snow Layers at Murodo, Tateyama, Japan
Kazuichi HAYAKAWA1,2*, Genki SUZUKI1, Fumiya MAKINO1, Hiroshi MORISAKI1, Ning TANG1,
Akira TORIBA1 and Kazuma AOKI3, 1Institute of Medical, Pharmacutical and Health Sciences,
Kanazawa University, Japan, 2Institute of Nature and Environmental Technology, Kanazawa University,
Japan, 3Graduate School of Science, Toyama University, Japan. E-mail: hayakawa@p.kanazawa-u.ac.jp

KL.23
Determination of Important Volatile Compounds from Aqueous Matrix
Sunyoung Bae*, Department of Chemistry, Seoul Women‟s University, Nowon-Gu,
Hwarangro, Seoul, Korea. E-mail: sbae@swu.ac.kr
ORAL PRESENTATIONS
OL.01
Electrochemical study on electron transfer across DNA base pairs
Jifeng Liu, School of Food Engineering and Biotechnology, Tianjin University of Scienceand Technology, Tianjin 300457, China. E-mail: China, jfliu@tust.edu.cn
OL.02
Application of novel nanomaterials in the phosphopeptide enrichment
Liping Li, Linnan Xu, Wen Ma, Huwei Liu, Yu Bai*, College of Chemistry and MolecularEngineering, Peking University, Beijing, China. E-mail: yu.bai@pku.edu.cn
OL.03
Antioxidant Activity of Volatile Compounds Isolated from Broccoli (Brassica oleracea L.)
sprouts by GC and GC-MS
Hae Won Jang*, Food Analysis Center, Korea Food Research Institute, Korea. E-mail:hwjkfri@kfri.re.kr
OL.04
２２

Water Quality Monitoring of Drainage River in Kuantan, Malaysia by using Ionexclusion/Ion-exchange Chromatography
Daisuke Kozaki1*, Norhasmira Idayu binti Harun1, Nobutake Nakatani2, Masanobu
Mori3, , Kazuhiko Tanaka3, 1Faculty of Industrial Sciences and Technology, University
Malaysia Pahang, Malaysia, 2Department of Environmental and Symbiotic Sciences,
College of Agriculture, Food and Environmental Science, Rakuno Gakuen University,
Japan, 3Graduate school of Science and Technology, Gunma University, Japan. E-mail:daisuke@ump.edu.my
OL.05
Analytical Applications of Higher-order Calibration Methods Based on Alternating
Multilinear Decomposition Algorithms
Hai-Long WU* and Ru-Qin Yu, State Key Laboratory of Chemo/Biosensing andChemometrics, College of Chemistry and Chemical Engineering, Hunan University,
Changsha 410082, China, E-mail: hlwu@hnu.edu.cn
OL.06
Preparation and Application of Microextraction Probe for Enrichment of Organics from
Aqueous Matrices
Hai-Fang Li*, Guilan Huang, Jin-Ming LIN, Department of Chemistry, Beijing Key
Laboratory of Micronalytical Methods and Instrumentation, Tsinghua University, Beijing
100084, China, E-mail: lihaifang@mail.tsinghua.edu.cn
OL.07
Multifunctional fluorescent optical sensors for bioanalysis
Yanqing Tian*, Department of Materials Science and Engineering, South University of
Science and Technology of China. E-mail: tianyq@sustc.edu.cn
OL.08
Development of a Hybrid Pyrolyzer for GAS CHROMATOGYAPHY
Naoki Oguri*, Toshio Tsuchiya, Kaoru Enokido, Tamami Takeda and Akira Onishi, Japan
Analytical Industry Co., Ltd. E-mail: n-oguri@jai.co.jp
OL.09
Facile preparation of a novel benyl imidazolium ionic liquids hybrid monolithic column
for residue analysis of nitrogen-heterocyclic pesticides by CEC
Yan Huang, Yankai Chen, Yanxia Tang, Xiaoping Wu*, MOE Key Laboratory of Analysis
and Detection Technology for Food Safety; College of Chemistry, Fuzhou University,
Fuzhou, China. E-mail: wapple@fzu.edu.cn
２３OL.10New strategies for the functionalization of polymer monolith and applications toseparation and preconcentration of organic compounds
Haijiao Zheng, Gang Chen, Qiong Jia*, College of Chemistry, Jilin University,
Changchun, 130012, China. E-mail: jiaqiong@jlu.edu.cn
OL.11
Electrodialytic Matrix Isolation and Preconcentration for Ionic Solutes
Shin-Ichi Ohira*, Takayuki Yamasaki, Kei Toda, Department of Chemistry, Kumamoto
University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. E-mail: ohira@sci.kumamotou.ac.jp
OL.12
Determination of arsenic species in food by LC-ICP-MS
Cheong-Tae Kim, Food Safety Research Institute, NONGSHIM CO., LTD, Seoul,
Korea, E-mail: kctmass@hanmail.net
OL.13
Electrothermal vaporization or atomization on tungsten coil for analytical atomic
spectrometry
Xue Jiang,a Chenghui Li,a Xiaoming Jiang,b Zhou Long,b Xiandeng Hou*a,b, aKey
Laboratory of Green Chemistry & Technology, Ministry of Education, College ofChemistry, Sichuan University, Chengdu, Sichuan 610064, China, bAnalytical & Testing
Centre, Sichuan University, Chengdu, Sichuan 610064, China. E-mail: houxd@scu.edu.cn.
OL.14
Functionalized Gold-Nanomaterials Fluorescence Probe for Cyanide Detection
Dongtao Lu; Guomei Zhang, Chuan Dong, Shaomin Shuang*, Institute of Environmental
Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan
030006, China. E-mail: smshuang@sxu.edu.cn
OL.15
Determination of silicate, borate and carbonate ions in natural waters using ion-exclusion
chromatography coupled with charged aerosol detector
Nobutake Nakatani*1, Yu Otsuka1, Takuya Takahashi1, Daisuke Kozaki2,
Masanobu Mori3, Kazuhiko Tanaka3, 1College of Agriculture, Food and Environment
Sciences, Rakuno Gakuen University, 2Faculty of Industrial Sciences and Technology,
University Malaysia Pahang, 3Graduate School of Science and Technology, Gunma
２４

University. E-mail: nakatani@rakuno.ac.jp
OL.16
Hairpin DNA Template-based Copper Nanoparticles for the Detection of NAD+
Jianzhong Lu*, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai,
China E-mail: jzlu@shmu.edu.cn
POSTERS PRESENTATIONS
PT.01
Development of Inkjet Printing Single-cell Technology to Electrospray Ionization for
Mass Spectrometer
Fengming Chen*, Shiqi Wang, Ziyi He, Hai-Fang Li, Jin-Ming LIN
Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods andInstrumentation,
Tsinghua
University,
Beijing
100084,China.
E-mail:chenfengmingok@mail.tsinghua.edu.cn
PT.02
Selective removal of cadmium from water samples by bio-functionalized composite
MT@C-dot@SiO2
Yi-Kun Li, Ting Yang, Ming-Li Chen*, Jian-Hua Wang, Research Center for Analytical
Sciences,
Northeastern
University,
Shenyang
110819,China.
E-mail:chenml@mail.neu.edu.cn
PT.03
Monitoring the dynamic mass-transferring behaviors of hemoglobinaqueous/ionic-liquid phases with liquid core optical waveguide system

between

Xu-Wei Chen*, Xu Yang, Wan-Ying Zeng, Jian-Hua Wang, Research Center for
Analytical Sciences, Northeastern University, Shenyang 110819, China, E-mail:chenxuwei@mail.neu.edu.cn

PT.04
Self-assembly sea urchin-like ZnO nanoparticles for headspace thin-film microextraction
coupled with SERS to detect sulfur dioxide in medlar
Zhuo Deng1*, Ziming Zhao1, Yiru Wang1, Xi Chen1,2, 1Department of Chemistry and the
MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College ofChemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China, 2StateKey Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005,
China. E-mail: joydeng2011@163.com
２５PT.05
Selectively enhanced molecular emission spectroscopy of benzene, toluene and xylene
with nano-MnO2 in atmospheric ambient temperature dielectric barrier discharge
Xue Jiang 1, Chenghui Li1, Xiandeng Hou1,2, 1Department of Chemistry, Sichuan
University, Chengdu, 610064, Chinam, 2Analytical & Testing Center, Sichuan University,
Chengdu, 610064, China. E-mail: jiangxue218@gmail.com
PT.06
Synthesis of Highly Fluorescent P-g-C3N4 Nanodots for Label-free Detection of Cu2+ andAcetylcholinesterase Activity
Mingcong Rong1, Chunhua Ma2, Zhaochai Qiu1, Xi Chen, 1Department of Chemistry andthe MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College ofChemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China,
2
College of tea and food science, Wuyi University, Wuyishan 354300, China. E-mail:haiyangzhixinrmc@126.com
PT.07
Simultaneous detection of dihydroxybenzene isomers by HKUST-1 MOF modified
electrode
Yizhen Yang, Qingxiang Wang*, College of Chemistry and Environment, Minnan
Normal University, Zhangzhou 363000, P. R. China. E-mail: axiang236@126.com
PT.08
In-situ growth of gold nanoparticles on mercury-binding M13 phage for Hg2+ sensing
Xiao-Yan Wang, Ming-Li Chen, Ting Yang*, Jian-Hua Wang, Research Center for
Analytical Sciences, Northeastern University, Shenyang 110819, China. E-mail:yangting@mail.neu.edu.cn

PT.09
Absorption of Pb2+ and Cd2+ from Water using 3D nitrogen-doped graphene
Ting-Ting ZHAO1, Feng LUO2, Xin-Hong SONG3, Xi CHEN3, 1Planning
Department,Xiamen Huaxia University, Xiamen 361024,China, 2Fujian Research Instituteof Metric Science; Fuzhou 350003, China, 2Quanzhou Medical College, Quanzhou,
362010, 3Department of Chemistry and the MOE Key Laboratory of Spectrochemical
Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen
University, Xiamen 361005,China. E-mail: ztt@hxxy.edu.cn

２６

PT.10Online multichannel ultrasonic extraction for high throughput determination of arsenic insoil by hydride generation atomic fluorescence spectrometry
Yuefen Zhang, a Lan Wu, a,b Chengbin Zheng,a Xiandeng Hou, a,b and Kailai Xu*a, aKey
Laboratory of Green Chemistry & Technology, Ministry of Education, College ofChemistry, Sichuan University, Chengdu, Sichuan 610064, China, bAnalytical & Testing
Centre, Sichuan University, Chengdu, Sichuan 610064, China. E-mail:xukailai@scu.edu.cn
PT.11
Label-free detection of microRNA by Au nanoparticles decorated reduced graphene oxide
field-effect transistor biosensor
Bingjie Cai, Guo-Jun Zhang*, School of Laboratory Medicine, Hubei University ofChinese Medicine, Wuhan 430065, China. E-mail: gjzhangtj@hotmail.com
PT.12
GC/MS analysis and sensory evaluation of odor compounds in environmental tobaccosmoke (ETS)
Hiroshi Sato1)*, Masahiro Oka1), Shuhei Nagahashi1), Kaori Imi1), Naoki Mizoguchi1),
Hidetaka Matsubara2), Miyuki Noguchi3), Keiichi Arashidani4)
1)
Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki International
University, 2)Chuken Laboratory for Life and Environment, 3)Faculty of Science andTechnology, Seikei University, 4)School of Health Sciences, University of Occupational
and Environmental Health. E-mail: satoh@niu.ac.jp
PT.13
Application of HPLC by Using Mass Spectrometry Detector
Masaki Watanabe*, Masaki Yoshie, Norimasa Minamoto, Kousaku Toyosaki
Optical Instrument Engineering Department, Hitachi high-tech Science Corporation, 1040
Ichige, Hitachinaka, Ibaraki 312-0033 Japan. E-mail: masaki.watanabe.yk@hitachihightech.com
PT.14
Analytical method for arsenic speciation in foods using HPLC-ICP/MS
Dongwon Seo*, Soyoung Kim, Eunjoo Park, Jisu Park, Jaeho Ha, Tae-Gyu Nam,
Jooyoung Lee, Haewon Jang, Jinbong Hwang, Food Analysis Center, Korea Food
Research Institute, Seongnam 463-746, Korea. E-mail: dwseo@kfri.re.kr
PT.15
２７

Nutritional composition of bamboo shoots (Phyllostachys Bambusoides Sieb. et Zucc.) in
Korea
Dongwon Seo1*, Songjin Lee2, Jinbong Hwang1, Kinjin Kim1, Soyoung Kim1, Eunjoo
Park1, Hyunjun Lee1, Haebin Cho1, Seunghee Kim1, Wonjin Yoon1, Jaeho Ha1, 1Food
Analysis Center, Korea Food Research Institute, Seongnam 13539, Korea, 2Bamboo
Resource Research Institute, Damyang 57365, Korea. E-mail: dwseo@kfri.re.kr
PT.16
Comparison of Macro Mineral Contents following Kind of Vegetables produced in Korea
Eun-joo Park1* , Soyoung Kim1, Jinbong Hwang1, Dongwon Seo1, Youngmin Choi2, SeNa Kim2, Jin-Sik Nam3, 1Department of Food analysis, Korea Food Research Institute,Gyeonggi, 13539, Korea, 2Functional Food & Nutrition Division, National Academy ofAgricultural Science, RDA, Jeonju, 04535, Korea, 3Department of Food & Nutrition,
Suwon Women's University, Gyeonggi, 18333, Korea. E-mail: Hwangjbg@kfri.re.kr
PT.17
Single molecule real-time imaging of intracellular Mg2+ signaling and wave propagationin single cell with subdiffraction-limit resolution
Suresh Kumar Chakkarapania, Seungah Leeb, and Seong Ho Kanga,b,*, aDepartment ofChemistry, Graduate School, Kyung Hee University, bDepartment of Applied Chemistry,
College of Applied Science, Kyung Hee University. E-mail: shkang@khu.ac.kr

PT.18
Novel detecting method of synthetic organic dyes by dual non-aqueous capillaryelectrophoresis
Moonhee Parka, Seung-Hoon Bahangb, Seong Ho Kanga,c,*, aDepartment of Chemistry,
Graduate School, Kyung Hee University, bCriminal Investigation Command of MND,
Seoul 140-701, Republic of Korea, cDepartment of Applied Chemistry, College of Applied
Science, Kyung Hee University, E-mail: moonhee@khu.ac.kr
PT.19
Biomolecular quantification in axial nanoscale of plasmonic particle on nanoisland
Seungah Leea, Sujin Ahnb, Seong Ho Kanga,b,*a
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446701, Republic of Korea, bDepartment of Chemistry, Graduate School, Kyung Hee
University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea. E-mail:shkang@khu.ac.kr

２８

PT.20Time-Series Variation of Atmospheric Radon-222 Concentrations at Gosan Site in Jeju
Island of Korea during 2012-2014
Jung-Min Song1, Jun-Oh Bu1, Won-Hyung Kim1, Chang-Hee Kang1*, S. Chambers2,
1
Division of Chemistry and Cosmetics, Jeju National University, Jeju 690-756, Korea,
2
Australian Nuclear Science and Technology Organisation, NSW 2232, Australia. E-mail:changhee@jejunu.ac.kr
PT.21
Effect of Asian Dust Storms on Atmospheric Aerosol Compositions in Background Site of
Korea during 2012-2014
Jun-Oh Bu1, Jung-Min Song1, Jae-Yun Lee1, Jong-Heon Han1, Chang-Hee Kang1*,
1
Division of Chemistry and Cosmetics, Jeju National University, Jeju 690-756, Korea. Email: changhee@jejunu.ac.kr
PT.22
Verification of Applicability of the Validated Analytical Method with a Liquid
Chromatography-Tandem Mass Spectrometry for Steroids Residues Screening in Food
Products
Hwa-Mi Lee*, JaeHyun Kim, WooJin Jeon, SooJin Bang, SungMin Woo, JuYeun Lee,
EunJoo Koo, JyongEun Myung, HyunUk Kim, EunYoung Lee, Soo Jung Hu, Young-Mi
Jang, Chul Joo Lim, Center for Food & Drug Analysis, Gyeongin Regional Food andDrug Administration, 137, Juan-Ro, Nam-Gu, Incheon-City, 402-835, Republic of Korea.
E-mail: lhm9904@gmail.com
PT.23
Method development for determination of heavy metals in turmeric powder using ICP-MS
Chang-Hee Lee, Yeon Kim, Myung-Ja Lee, Bock-Kyu Lim, Ki-Hyun Kim, Yeong-Hyeon
Kim,Tae-Yong Cho, Jae-Young Shim*, Center for Food & Drug Analysis, BusanRegional Korea Food and Drug Administration, Busan, Republic of Korea E-mail:shilvi0321@gmail.com
PT.24
Effect of Different Biomass on Value-added Products in Bio-liquid of Food Waste via
Hydrothermal Carbonization
Soomi Park1, Jonathan Lee2, Yura Hwang1, Soyoung Lee1, Sunyoung Bae1*
Department of Chemistry, Seoul Women‟s University Nowon-Gu, Hwarangro, Seoul,
Korea, 131, Davidson College 405 North Main Street, Davidson, NC 280352. E-mail:sbae@swu.ac.kr
２９

PT.25
Analysis of Polycyclic Aromatic Hydrocarbons in Water by Polyaniline Coated In-needle
Microextraction
Yura Hwang, Dong-Sun Lee, Sunyoung Bae* Department of Chemistry, Seoul Women‟s
University, Nowon-Gu, Hwarangro, Seoul, Korea. E-mail: sbae@swu.ac.kr
PT.26
TNF-α-induced insulin resistance mechanism study based on cell lysis-free quantum dot
multicolor cellular imaging-based
Jieun Ki and Joon Myong Song*, College of Pharmacy, Seoul National University, Seoul
151-742, South Korea. E-mail: jmsong@snu.ac.kr
PT.27
Real time high-content cellular monitoring of the cytotoxic action of cationic polymer
carriers polyethylenimine and poly-L-lysine on HepG2 cells
Gee Woo Nam and Joon Myong Song*, College of Pharmacy, Seoul National University,
Seoul 151-742, South Korea. E-mail: jmsong@snu.ac.kr
PT.28
Multifunctional Composite of an Antibacterial Silver and a Potent Wound Healing
Polypeptide as a Bimodal Wound Treatment
Baskaran Purushothaman and Joon Myong Song*, College of Pharmacy, Seoul
National University, Seoul 151-742, South Korea. E-mail: jmsong@snu.ac.kr
PT.29
Concurrent monitoring of CD31, CD34, CD45 and CD146 endothelial progenitor cellmarkers for acute myocardial Infarction
Hee Ju and Joon Myong Song*, College of Pharmacy, Seoul National University, Seoul
151-742, South Korea. E-mail: jmsong@snu.ac.kr
PT.30
Optimization of Chlorophenols Detection in Water Samples using Stir bar Sorptive
Extraction Coupled with Thermal Desorption-GC-MS
Mina Lee, Yun S. KIM*, Water Quality Reserch Centre, K-water Institute, K-water,
Korea. E-mail: yunskim@kwater.or.kr
PT.31
Validation of Analytical Method for Propylene Glycol and Ethylene Glycol in Foods
Ho Soo Lim, Ju Young Hwang, EunA Choi, Gun young Lee and Tae Seok Kang, Food
３０

Additives and Packaging Division, Ministry of Food and Drug Safety, Cheongju-si,chungcheongbuk-do, Korea. E-mail: hslim70@korea.kr
PT.32
Dielectric Barrier Discharge Molecular Emission Spectrometer as Gas Chromatographic
Detector for Amines
Chenghui Li a, Xue Jianga, Xiandeng Houa,b*, aDepartment of Chemistry, Sichuan
University, Chengdu, 610064, China, bAnalytical & Testing Center, Sichuan University,
Chengdu, 610064, China E-mail: houxd@scu.edu.cn
PT.33
Aptamer-bound magnetic beads and antibiotics functionalized fluorescent gold
nanoclusters based dual recognition strategy for detection of bacteria
Dan Cheng, Mengqun Yu, Gan Li, Zheguo Zhu, Yang Song, Erqun Song*, Key
Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education,
College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, People‟s
Republic of China. Fax: +862368251225; Tel: +862368251225. E-mail:eqsong@swu.edu.cn
PT.34
Determination of the fatty acid compositions offal meat of sheep produced in Mongoliausing GC and GC-MS
G.Oyun1, D.Narantuya1, E.Anudari1, D. Seo2, J. Ha 2,3*, 1Scientific food research andmanufacturing “SAMO” Institute, Ulaanbaatar, Mongolia, 2 Food Analysis Center, Korea
Food Research Institute, 3 Korea University of Science and Technology. E-mail:jhkfri@gmail.com

３１

PL.01
Study of DNA/Small Molecule Interaction by Dual Polarization
Interferometry
Xiurong Yang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied
Chemistry, Chinese Academy of Sciences, xryang @ciac.ac.cn
Abstract: Dual polarization interferometry (DPI) has been developed to give accurate
information of layer mass, refractive index (RI) and thickness. These facilities to reveal
structural information about the biomolecular interactions taking place as the packing(from the RI) and molecule dimension (from the thickness) change, and typically give alink between a molecule‟s structure and its function. Functional oligonucleotides havegained many interests owing to their specific recognition ability to analytes. The study of
DNA/small molecule interaction is very valuable for probing the mechanism of theinteraction and establishing convenient methods to effectively understanding the functionsof the small molecules.
DPI technique was used in our group to probe the interaction of deferent
biosystems,1,2,3,4,5,6 for example, the interaction of coralyne with 48-mer homoadenine
oligonucleotide (polyA48), silver ions with cytosine (C)-rich ssDNA oligonucleotides,
Hg2+ with 21-mer thymine-rich oligonucleotide (MSO) and so on. Kinetic, thermodynamic
and structural information of these interaction processes were characterized in detail.
Using the binding curves from changes in mass, the association rate constant (ka), thedissociation rate constant (kd) and the association constant (Ka) between coralyne-polyA48,
silver ions - cytosine (C)-rich ssDNA oligonucleotides and Hg2+-MSO were calculated,
respectively. Simultaneously, we constructed biosensors for respective selective detectionof coralyne, cytosine, Ag+ and Hg2+ and so on. The selectivity of the biosensor over theother corresponding substances was confirmed.
It is expected that the biosensing strategy will be extended for biosensing of other various
analytes by using other different functional oligonucleotides (aptamers or DNAzymes),simultaneously investigate the interaction between them and reveal the structral
characteristics of functional DNA with and without targets.
Acknowledgements.: This work was supported by the National Natural Science
Foundation of China (No. 21435005 and 21175124 )Keywords: Dual polarization interferometry, Biomolecole interaction, Biosensor
３２

Reference:
Xiurong Yang* et al, Chem. Commun.,51(2015) 5645-5648
Xiurong Yang* et al, Anal. Chem., 86(2014)3849−3855
Xiurong Yang* et al, Chem. Commun., 48(2012) 2873–2875
Xiurong Yang* et al, Anal. Chem. 84(2012) 924−930
Xiurong Yang* et al,Anal. Chem., 82(2010) 5455–5462
Xiurong Yang* et al, Anal. Chem., 81(2009) 4914–4921
Biography of the author.
Xiurong Yang: PhD, Professor, Academician of CAS
State Key Laboratory of Electroanalytical Chemistry,
Changchun Institute of Applied Chemistry,
Chinese Academy of Sciences
China
E-mail: xryang @ciac.ac.cn
Xiurong Yang is currently a professor in the State Key Laboratory of Electroanalytical
Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. She
received her B. S. from the University of Science and Technology of China (USTC), andPh.D. Degree from Lund University, Sweden. She had been the Post Doctoral Research
Fellow at the University of New Orleans and the University of Hawaii at Manoa, USA(1992-1995). She had been the Visiting Assistant Professor at the Hong Kong University of
Sciences and Technology, Hong Kong, China (1995-1997), and the Visiting Professor in the
University of Perpignan, France (2007). She had been the Director of the State Key
Laboratory of Electroanalytical Chemistry and its precursor (1998 - 2009). She is now theeditor in chief of Chinese J. Analytical Chemistry, and the Chair Person of the Analytical
Chemistry Committee, Chinese Chemical Society. Her research interests arenanoelectrochemistry, biomolecular recognision and interaction and so on.

３３

PL.02
Inkjet-printed analytical devices made from paper
Daniel Citterio* and Koji Suzuki
Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, 2238522 Yokohama, Japan
Abstract: Analytical devices made from paper are increasingly drawing attention, inparticular in the context of clinical diagnostics, environmental monitoring and workplace
condition surveillance. Since the first publication in the field by Whitesides and coworkers
in 2007,1 research activities have grown tremendously. A common feature of all paperbased analytical devices (PADs) is the perspective to have low-cost, portable and userfriendly alternatives to the common large-scale analytical systems requiring a high level ofinfrastructure and technically trained operators.
The primary reasons for choosing paper as the base material of PADs are its low
cost, abundant availability and easiness of disposal. But at the same time, the physicalchemical properties of paper make it a versatile functional material with characteristics not
so straightforwardly achieved with more presumably “high-tech” materials. The two mostimportant properties are the autonomous pump-free sample transport due to capillary
forces in the porous paper structure in the context of microfluidic paper-based analyticaldevices (µPADs), and the large surface-to-volume ratio allowing for the immobilizationand dry storage of assay reagents.
The use of inkjet technology is no longer limited to classical office printing
applications. It enables the rapid, reproducible and contactless dispensing of picoliterorder liquid droplets at defined spots on a variety of surfaces. In this way, it is suitable forthe deposition of a wide range of materials required for (bio)chemical assays.2
Furthermore, by directly enabling the transfer of electronically stored information from acomputer to a paper surface, inkjet printing allows the fabrication of microfluidicallypatterned surfaces with great flexibility in pattern design, without the need to use masks.
The total number of required process steps is reduced, and as a further advantage, all steps
can be performed using an inkjet printer. As a consequence, inkjet printing has become apopular tool in the development and fabrication of paper-based analytical devices.3
Since 2008, our research group is focusing on the application of inkjet printing
technology for the development of paper-based (bio)analytical devices.4-9 We have been
the first to demonstrate how an inkjet printing system can be used for both themicrofluidic patterning of paper surfaces and the deposition of assay reagents to obtainµPADs.4, 5 Later on, we have developed an alternative inkjet-based patterning approach for３４paper substrates relying on a solvent-free UV-light curable hydrophobic monomer
composition, which is not only more environmentally friendly compared to our previous
method, but more importantly, which can be printed from the most simple unmodified
desktop inkjet printer.6 This technology has more recently been applied to the fabricationof a µPAD for the determination of the glycoprotein lactoferrin in human tear fluid
samples, where the detection mechanism relies on the sensitization of terbium(III)fluorescence upon binding of the cation used as the indicator to the metal-ion binding siteof the protein.7, 8 The accuracy of the developed µPAD has been evaluated by comparing
the analysis results of human tear samples with the conventional enzyme-linked
immunosorbent assay (ELISA) method and a good correlation between the two analyticalmethods has been confirmed. Compared to the ELISA approach, the strengths of theµPAD assay have been demonstrated in terms of simplicity, speed and cost.
We have also demonstrated that the combination of inkjet printing and paper isalso suitable for the fabrication of non-microfluidic systems. A colorimetric sensor arrayfor the discrimination of volatile amines has been developed by combining two simple
functional elements: (1) a single chromogenic sensing dye with selectivity for amines ingeneral, encapsulated into (2) polymer nanoparticles of different polarities. Printing of 6
types of inks incorporating the two dye encapsulating nanoparticles of different polarity indifferent mixture ratios onto plain copy paper resulted in a colorimetric sensor array with apolarity gradient. This array indicated high discrimination ability for 7 volatile amines
with good selectivity.9
This lecture will give a short overview of our developments targeting inkjet-printed
analytical devices made from paper. Some general approaches as well as specificchallenges and potential solutions for inkjet-printable “chemical sensing inks” will beshown.
Keywords: microfluidic device, colorimetric analysis, (bio)chemical sensor
References:
1. Martinez, A. W.; Phillips, S. T.; Butte, M. J.; Whitesides, G. M. Angew. Chem., Int. Ed.
2007, 46, 1318-1320.
2. Komuro, N.; Takaki, S.; Suzuki, K.; Citterio, D. Anal. Bioanal. Chem. 2013, 405, 57855805.
3. Yamada, K.; Henares, T. G.; Suzuki, K.; Citterio, D. Angew. Chem., Int. Ed. 2015, 54,5294-5310.
4. Abe, K.; Suzuki, K.; Citterio, D. Anal. Chem. 2008, 80, 6928-6934.
5. Abe, K.; Kotera, K.; Suzuki, K.; Citterio, D. Anal. Bioanal. Chem. 2010, 398, 885-893.
6. Maejima, K.; Tomikawa, S.; Suzuki, K.; Citterio, D. RSC Adv. 2013, 3, 9258-9263.
7. Yamada, K.; Takaki, S.; Komuro, N.; Suzuki, K.; Citterio, D. Analyst 2014, 139, 1637３５

1643.
8. Yamada, K.; Henares, T. G.; Suzuki, K.; Citterio, D. submitted for publication 2015.
9. Soga, T.; Jimbo, Y.; Suzuki, K.; Citterio, D. Anal. Chem. 2013, 85, 8973-8978.
Introduction to the author:
Professor Dr. Daniel Citterio
Department of Applied Chemistry, Keio University, Yokohama, Japan
Tel/Fax: +81-45-566-1568.
E-mail: citterio@applc.keio.ac.jp
Biography of the authors.
Daniel Citterio received his Doctor of Natural Sciences degree in 1998 from the Swiss
Federal Institute of Technology (ETH) in Zurich. After postdoctoral research at Keio
University in the group of Prof. Dr. Koji Suzuki, he became a Research Associate at ETH
in 2002. Following post-graduate studies and work at Ciba Specialty Chemicals, hereturned to Keio University in 2006, where he became a tenured Associate Professor in2009 and full Professor in Analytical Chemistry in 2014. His research interests are thedevelopment of low-cost analytical devices and the development of functional moleculesfor (bio)chemical sensing.
Koji Suzuki received his PhD degree in 1982 from Keio University. He became afaculty member as a Research Associate in 1982, Assistant Professor in 1988, Associate
Professor in 1993, and full Professor in 1998. From 1990 – 1992, he was a Guest
Professor at the Swiss Federal Institute of Technology (ETH), Zurich. He served as theVice President of JSAC for 2011 – 2013 and as President since 2015. His research focuses
on chemical and biochemical sensors based on functional molecule creation.

３６

PL.03
Determination of trans fatty acids in daily consumed foods using a silver
ion cartridge solid phase extraction method coupled with GCxGCTOFMS
Dongwon Seo 1, Jaeho Ha 1,2*
1

Food Analysis Center, Korea Food Research Institute2
Korea University of Science and Technology
Abstract: This study aimed to develop an analytical method for accurately estimating theratio of vaccenic to elaidic acid (V/E value). To estimate an accurate V/E value, a silver
ion cartridge (SIC) solid phase extraction (SPE) method was performed for removing cisfatty acids. The removal efficiency of cis-fatty acids was greater than 97.8%. However,
the analytical values of total trans-fatty acids (TFAs) were not significantly different
between with SIC SPE method and without SIC SPE method. Comparison tests of the V/Evalues produced by two different methods (without SIC SPE method and with SIC SPE
method) using comprehensive two-dimensional gas chromatography combined with timeof-flight mass spectrometry (GC × GC-TOFMS) were also carried out. Those two
methods exhibited significant differences in the estimated V/E values because removing
cis-fatty acids affected the V/E value but did not affect the total TFAs. The SIC SPE
method coupled with GC × GC-TOFMS suggested in this research is available to estimate
accurate V/E values in commonly consumed foods, such as margarines, milk products andbeef tallow.
Keywords: Elaidic acid, Vaccenic acid, Silver ion cartridge, GC × GC-TOFMS
Reference:
1. Talanta 85 (2011) 252–258Talanta 85 (2011) 252–258
2. R.G. Ackman, S.N. Hooper, D.L. Hooper, J. Am. Oil Chem. Soc. 51 (1974) 42–49.
3. J.L. Beare-Rogers, L.M. Gray, R. Hollywood, Am. J. Clin. Nutr. 32 (1979) 1805–1809.
4. S.M. Innis, Atheroscler. Suppl. 7 (2006) 17–20.
5. C. von Muhlen, C.A. Zini, E.B. Caramao, P.J. Marriott, J. Chromatogr. A 1105 (2006)
39–50.
Introduction to the author:
Professor Dr. Jaeho HA
３７

Food Analysis Center, Korea Food Research Institute, 463-746, Korea
Tel: +82-31-780-9217; Fax: +82-31-780-9280.
E-mail: jhkfri@gmail.com
Biography of the authors.
Dr Jaeho Ha completed his doctorate in food chemistry with the practical
combination of mass spectrometry and gas chromatography at the Korea University in
1992. He joined the Food Analysis Center at Korea Food Research Institute in 1984,
where he involved himself in the analysis of food components, including micotoxin,
pesticides, nutrients, fatty acid and aromas. Dr. Ha served his vice-presidency of Korean
Society of Analytical Science 2000 – present. He is currently the vice president of Korea
Food Research Institute and a leader of food analysis division of Korea Society of Food
Science and Technology. Concurrently, Dr. Ha is applying GCxGC-TOFMS technology
coupled with stir bar sorptive extraction and dynamic headspace sampling techniques forthe analysis of flavors and trans fatty acids in foods. His research and activity is focusedon chromatographic separation, flavor chemistry, food analysis.

３８PL.04Generation of Droplets of Liquid for Mass Spectrometry
Jin-Ming Lin1, Chen Luo1, Yangdong Zhang1, Fengming Chen1,2, Katsumi Uchiyama2
1

Department of Chemistry, Tsinghua University, Beijing 100084, China
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences,
Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0364, Japan
E-mail: jmlin@mail.tsinghua.edu.cn
2

Abstract: In this work, a paper spray mass spectrometry combined with droplets generated
by gravity and electrostatic attraction, microfluidic chip, and inkjet methods, wasdeveloped. The qualitative and quantitative analytical performances of this technique forsingle droplet were demonstrated. This manually controlled interface is straightforward,
low-cost and simple to implement. Moreover, paper spray ionization mass spectrometry
hold promises in direct analysis of real biological/chemical microreaction samples becauseof its tolerance with complex matrix. As a proof-of-concept example, the droplet-based
acetylcholine hydrolysis was carried out to demonstrate the validation of our method for
direct analysis of micro-chemical/biological reactions. A module for cell culture was
integrated to investigate cell metabolism by online microfluidic chip-MS, employing
lactate efflux as a model. Three aspects were focused: real-time monitoring of the cellular
metabolites, differential effects of a drug on cells of different types, and the dose-response
information of a drug. The potential of this platform in cell analysis was exhibited.
Acknowledgements.: This work was supported by National Natural Science Foundationof China (No. 21227006, 91213305) and CERS-China Equipment and Education
Resources System (No. CERS-1-75).
Keywords: Droplets, Mass spectrometry, Paper spray ionization
Reference:[1] J. Liu, H. Wang, N.E. Manicke, J.-M. Lin, R. G. Cooks, Z. Ouyang, Development,characterization, and application of paper spray ionization. Anal. Chem., 2010, 82(6),2463-2471.
[2] Y. Zhang, H. Li, Y. Ma, J.-M. Lin, Paper spray mass spectrometry based method foranalysis of droplets in a gravity-driven microfluidic chip, Analyst, 2014, 139 (5), 1023 –1029.
[3] W. Liu, S. Mao, J. Wu, J.-M. Lin, Development and applications of paper-sprayionization-mass spectrometry for continuous sub-microlitre droplets analysis, The Analyst,
３９

2013, 138(7), 2163-2170[4] W. Liu, N. Wang, X. Lin, Y. Ma, J.-M. Lin, Interfacing micro-sampling droplets andmass spectrometry by paper spray ionization for online chemical monitoring of cell
culture, Anal. Chem., 2014, 86 (14), 7128–7134.
[5] C. Luo, Y. Ma, H. Li, F. Chen, K. Uchiyama, J.-M. Lin, Generation of pico-liter
droplets of liquid for electrospray ionization with piezoelectric inkjet, J. Mass Spectr.,
2013, 48, 321-328.
[6] F. Chen, Y. Rang, Y. Weng, L. Lin, H. Zeng, H. Nakajima, J.-M. Lin. K. Uchiyama,
Drop-by-drop chemical reaction and sample introduction for capillary electrophoresis,
Analyst, 2015, 140, 3953–3959[7] W. Liu, Q. Chen, X. Lin, J.-M. Lin, Online multi-channel microfluidic chip-mass
spectrometry and its application for quantifying noncovalent protein-protein interactions,
Analyst, 2015, 140, 1551-1554.
Biography of the author.
Jin-Ming Lin, PhD, FRSC
Cheung Kung Scholars Professor
Department of Chemistry
Tsinghua University
China
E-mail: jmlin@mail.tsinghua.edu.cn
Website: http://www.linlab-tsinghua-edu.org

Professor Dr. Jin-Ming Lin was graduated from Fuzhou University in 1984 and received a
PhD at Tokyo Metropolitan University in 1997. He had studied and worked in Showa
University and Tokyo Metropolitan University during 1992-2002. He was selected as “100Talented Researcher Program” for Chinese Academy of Sciences at 2001 and worked in
Research Center for Eco-Environmental Sciences, Chinese Academy of Science during
2002-2004. He is currently a professor of Tsinghua University since 2004. He is a
General-in-Secretary of the Committee of Mass Spectrometry, a Fellow of Royal Societyof Chemistry, a member of council of Chinese Society for Chromatography Science, andservice as Contributing Editor of Trends in Analytical Chemistry, Associate Editor of
Journal of Pharmaceutical Analysis, and Associate Editor of Current Analytical Chemistry,
Editorial Boards for Talanta, Toxicological and Environmental Chemistry, Luminescence,
Chinese Chemical Letters, Science China Chemistry. He received more than 20 awards for４０his contributions in microfluidics, chemiluminescence and separation science. He is theauthor and co-author of 358 original research papers published in international journals,
28 reviews, 4 books and 41 patents.
His current research is focused on microfluidic device for cell analysis, ultra weakchemiluminescence, and sample pretreatment for LC-MS and GC-MS.

４１

KL.01Gold-containing Nanocarriers/Nanocomposites for Anti-bacterial
Applications
Bo Hu, Ning Wang, Ming-Li Chen, Xu-Wei Chen, Jian-Hua Wang*
Research Center for Analytical Sciences, Northeastern University, Shenyang 110819,
China
It is known that gold nanorods (Au NRs) are generally capsules with anisotropic
structure. Au NRs and their composites have been demonstrated to exhibit superb antibacterial effects. Thus, we report herein the development of gold nanorods or gold-silver
nanocomposites for anti-bacterial applications and controlled drug release.
We constructed a hybrid bactericidal material, i.e., gold nanorods covered kanamycinloaded hollow-SiO2 (HSKAurod) nanocapsules. The hybrid combines the features ofchemical drug and photo-thermal physical sterilization. Hollow-SiO2 nanocapsules areused as carrier for drug delivery. The nanocapsules load kanamycin and are covered withgold nanorods to avoid drug leakage and realize photo-thermal treatment. The sterilizing
effect to bacterial strain is investigated with E. coli BL21 under irradiation of near-infrared
light (NIR) for 20 min. A sterilizing rate of 53.47% is achieved for the HSKAurod
nanocapsules with respect to a net sum sterilizing rate of 34.49% for the individualcomponents in the HSKAurod nanocapsules, e.g., carrier nanocapsules, chemicalsterilization of kanamycin and physical sterilization due to the gold nanorods under NIR
irradiation.
A novel bactericidal material, e.g., rod-shaped core-shell-shell Au-Ag-Au
nanorods, is constructed as a nanoheater in the NIR region. The outer Au shell melts underlaser irradiation and results in exposure of the inner Ag shell facilitating controlled releaseof the antibacterial Ag shell/layer. This offers the Au-Ag-Au nanorods a favorable
bactericidal ability by combining the features of physical photothermal ablation
sterilization of the outer Au shell and the antibacterial effect of the inner Ag shell to thesurrounding bacteria. The sterilizing ability of Au-Ag-Au nanorods is investigated with E.
coli O157:H7. Under low-power NIR laser irradiation (785 nm, 50 mW cm-2), the Au-AgAu nanoheater exhibits higher photothermal conversion efficiency with respect to that for
the Au-Ag nanorods. Meanwhile, a much improved stability with respect to Au-Ag
nanorods is observed, i.e., a 16 days successive monitoring reveals virtually no change for
the UV-vis spectrum of Au-Ag-Au nanorods. At a dosage of 10 μg mL-1, a killing rate of４２

100% is reached for the E. coli O157:H7 cells under a 20-min irradiation. The bimetallic
core-shell-shell nanorods provide a favorable nanoheater for in vivo biomedical
applications.
The Au-Ag-Au nanorods have been further used for bacteria capture, separationand sterilization (killing). For this purpose, positively charged magnetic reduced graphene
oxide with modification by PEI (rGO-Fe3O4-PEI) is prepared and then loaded with coreshell-shell Au-Ag-Au nanorods to construct a nanohybrid rGO-Fe3O4-Au-Ag-Au. NIR
laser irradiation melts the outer Au shell and exposes the inner Ag shell facilitating
controlled release of the silver shell. The nanohybrids combine physical photothermal
sterilization due to the outer Au shell and antibacterial effect of the inner Ag shell. In
addition, the nanohybrid exhibits high heat-conductivity due to rGO and rapid magnetic
separation capability attributed to Fe3O4. It provides a significant improvement onbactericidal efficiency with respect to bare Au-Ag-Au nanorods and facilitates theisolation of bacteria from sample matrixes. 25 μg mL-1 of nanohybird causes a 100%
capture and separation of E. coli O157:H7 (1×108 cfu mL-1) from aqueous medium in 10
min. Meanwhile it causes a 22oC temperature rise for the surrounding solution under NIR
irradiation (785 nm/50 mW cm-2) for 10 min. With magnetic separation, 30 μg mL-1 ofnanohybrid results in 100% killing rate for E. coli O157:H7 cells. The facile bacteria
separation and photothermal sterilization is potentially feasible for environmental and/or
clinical treatment.
Reference:
B. Hu, L.-P. Zhang, X.-W. Chen and J.-H. Wang, Nanoscale, 5, 246-252 (2013)
B. Hu, N. Wang, L. Han, M.-L. Chen and J.-H. Wang, Acta Biomater., 11, 511-519 (2015)
B. Hu, N. Wang, L. Han, M.-L. Chen and J.-H. Wang, Chem-Eur. J., 21, 6582-6589 (2015)
Introduction to the author:
Professor Dr. Jian-Hua Wang
Research Center for Analytical Sciences, Box332, Northeastern University, Shenyang
110819, China
Tel: +86-24-83688944; Fax: +86-24-83676698.
E-mail: jianhuajrz@mail.neu.edu.cn
Professor Jian-Hua Wang was born in 1962 in Shandong, China. He obtained his B.Sc
from Department of Chemistry, Nankai University, and M.Sc from Jilin University, China.
He was awarded a PhD degree by Technical University of Denmark. He has been an
Associate Editor for TALANTA since Dec. 2004. He was a member of International
Advisory Board for Journal of Analytical Atomic Spectrometry (2006-2010).
４３

His current research interest is focused on the exploitation of flow analysis protocols andhyphenation techniques, isolation of trace level of analytes (metals andbiomacromolecules) from complex sample matrices and life science analysis.

４４KL.02The synthesis of fluorescent graphitic carbon nitride nanomaterials andtheir application in fluorescence sensing

Xi Chen1,2*, Mingcong Rong1, Yiru Wang1
1. Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis
and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University,
Xiamen 361005, China
2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen
361005, China
Corresponding author email: xichen@xmu.edu.cn
Abstract:
Graphitic carbon nitride (g-C3N4) is a typical metal-free polymeric semiconductor,
which received a lot of attention due to its special structure and properties.1Since its
discovery, it has been widely used in the fields of sensing, bioimaging, peroxidase-likecatalysis and phtoelectrocatalysis.2 In recent years, rise with the development of carbonbased nanomaterials, nanoscale g-C3N4 materials, such as fluorescent g-C3N4 nanosheetsand g-C3N4 nanodots, have drawn much attention because of their excellent optical andphysicochemical features. The bulk g-C3N4 is usually obtained by high-temperature
pyrolysis of small organic molecules with high content nitrogen, including melamine,
cyanamide, dicyandiamide, urea and so on. There are two ways to get nanoscale g-C3N4.
One is the up-to-down method, which include liquid phase exfoliation and chemical
oxidation and hydrothermal etching of bulk g-C3N4. Another is the down-to-up method,which contains hydrothermal pyrolysis and microwave of organic molecules with highcontent nitrogen to form the s-triazine or tri-s-triazine structure of g-C3N4. The uniqueproperty of nanoscale g-C3N4and their potential application in fluorescence sensing also
aroused our interest.
With the chemically oxidized and liquid exfoliatedg-C3N4 nanosheets, firstly, wedeveloped a facile and effective fluorescence “on–off” sensing approach for the label-freeselective detection of Cr(VI) based on the inner filter effect and a fluorescence “on–off–on”
strategy for the determination of AA via the oxidation–reduction between Cr(VI) and AA.
The developed approach provided a wide linear range of 0.6 to 300 μM for Cr(VI) and 0.5
to 200 μM for AA, with a low detection limit of 0.15 and 0.13 μM for Cr(VI) and AA. 3
Secondly, using g-C3N4nanosheets, we investigated their application in the determination
４５

of 2,4,6-trinitrophenol (TNP). The strong inner filter effect and molecular interactions(electrostatic, π−π,and hydrogen bonding interactions) between TNP and the gC3N4nanosheets led to the fluorescence quenching of the g-C3N4nanosheets with efficient
selectivity and sensitivity. Under optimal conditions, a detection limit of 8.2 nM was
obtained.4 The relative low quantum yield (no more than 30%) and large size (commonly
70 to 160 nm) of mostly g-C3N4 nanosheets are always big problems that limit theirapplications in imaging. By chemical oxidation and hydrothermal etching of bulk P-gC3N4 obtained via pyrolysis of phytic acid and melamine, we synthesis a highly
fluorescent phosphorus-doped graphitic carbon nitride nanodots (P-g-C3N4 nanodots). The
P-g-C3N4 nanodots emitted strong blue fluorescence and displayed highly fluorescentresponses towards Cu2+ by photo induced electron transfer. Under optimal conditions, a
rapid detection of Cu2+ could be completed in 5 min with a detection limit of 2 nM, and alinearity ranging from 0 to 1 μM. Meanwhile, using acetylthiocholine as the substrate, theP-g-C3N4 nanodots-Cu2+ system could sensitively detect acetylcholinesterase through thereaction between Cu2+ and thiocholine, the hydrolysis product of ATCh by AChE. A
linearity ranging from 0.01 to 3 mU/mL could be obtained with a detection limit of 0.01
mU/mL.
Key words: graphitic carbon nitride (g-C3N4); fluorescence detection; 2,4,6-trinitrophenol(TNP); Cr(VI); AA; P-g-C3N4 nanodots; AChE
References:(1) Wang, Y.; Wang, X.; Antonietti, M. Angew Chem Int Ed 2012, 51, 68-89.
(2) Tang, Y.; Su, Y.; Yang, N.; Zhang, L.; Lv, Y. Analytical chemistry2014, 86, 4528-4535.
(3)Rong, M.; Lin, L.; Song, X.; Wang, Y.; Zhong, Y.; Yan, J.; Feng, Y.; Zeng, X.; Chen, X.
Biosensors & bioelectronics2015, 68, 210-217.
(4)Rong, M.; Lin, L.; Song, X.; Zhao, T.; Zhong, Y.; Yan, J.; Wang, Y.; Chen, X.
Analytical chemistry2015, 87, 1288-1296.

４６

Professor Xi Chen
Prof. Dr. Xi Chen
Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis &Instrumentation, College of Chemistry and Chemical Engineering
Xiamen University
Xiamen 361005, China========================================================
Tel/Fax: +86(592) 2184530
E-mail: xichen@xmu.edu.cn
Dr. Xi Chen graduated from Fuzhou University with a Bachelor‟s degree of analyticalchemistry, and then obtained his Master‟s degree there. Later in 1991, awarded the
Monbusho Scholarship from Japanese government, he entered Kyoto Institute ofTechnology and received his Ph.D degree four years later. Between the year of 1996 and1998, he performed as a postdoctoral researcher at Xiamen University, sooner after which,he was promoted as an associate professor at the department of chemistry. In 2003, he waspromoted to be a full professor. He got the financial support from Japanese Society for the
Promotion of Science (JSPS) to collaborate with Prof. M. Oyama, Kyoto University, in2004 and 2009. Dr. Chen has taken charge of projects as National High TechnicalDevelopment Project (863 Project) Foundation in the field of ocean, National ScientificFoundation of China and Key Project of Fujian Scientific Foundations, He was honored
the Outstanding Contribution Award in 2002, and received the 1st class Scientific Progress
Prize of Fujian province in 2005. His research interests cover electrochemical analysis
using graphene materials, chemo/biosensors and pre-extraction materials applied in
GC/HPLC. He has published over 160 scientific research articles in archival scientificjournals, including Angew. Chem. Int. Ed., J. Am. Chem. Soc. and Anal. Chem.

４７

KL.03
Simultaneous and selective determination of inorganic nitrogen species
in seawater samples
Kazuaki Ito1
1

Department of Biotechnology and Chemistry, Faculty of Engineering, Kinki University, 1
Umenobe, Takaya, Higashi-Hiroshima, 739-2116, JAPAN
Abstract: Inorganic nitrogen compounds (ammonium, nitrite, and nitrate ions) are
important measurement items because the ions are related to the phenomena such as
eutrophication in water environment. Thus, a number of measurement methods have been
examined for the determination of the ions. However, the determination by conventional
measurement system is interfered by excess of matrix ions in seawater. In this study,
determination of ammonium ions in seawater samples was examined by indophenol blue
reaction method and OPA method. And, the system optimized for ammonium detection
was incorporated as post column reaction system on ion chromatography (IC): Nitrite andnitrate ions were separated on ODS column equilibrated with DDAB
(dilauryldimethylammonium bromide) or anion exchange column and were detected by
UV method. The optimization of total system was examined for the determination of
inorganic nitrogen species in seawater and the method obtained was applied to real
samples.
Keywords：ion chromatography; post-column reaction; nitrite, nitrate, and ammonium
ions; seawater.
Introduction to the author:
Professor Dr. Kazuaki Ito
Department of Biotechnology and Chemistry, Faculty of Engineering, Kinki University,
Higashi-Hiroshima 739-2116, Japan
Tel: +81-82-434-7000; Fax: +81-434-7890. E-mail: itok@hiro.kindai.ac.jp
Biography of the author.
Kazuaki Ito, Research interest, ion chromatography for trace ions/ components in various
samples.

４８KL.04

Sputtered nanocarbon film electrodes for bioelectroanalysis

Dai Kato* and Osamu Niwa
National Institute of Advanced Industrial Science and Technology (AIST), Higashi,
Tsukuba, Ibaraki 305-8566, Japan
Abstract:
We have been studying nanocarbon film electrodes formed by an electron
cyclotron resonance (ECR) sputtering or an unbalanced magnetron (UBM) sputtering
method.1) The film provides a nanocrystalline sp2 and sp3 mixed bond structure with an
atomically flat surface (surface roughness of 0.05 – 0.1 nm) and high conductivity (20-38S/cm) without doping. The film electrode has excellent properties including a low
background current, a wide electrochemical potential window, and little surface fouling,
while maintaining relatively high electrode activity for various biomolecules.2) These
characteristics allow the detection of biomolecules, especially ultratrace amount of
biomolecules such as all the DNA bases, gliotransmitters, and lipopolysaccharide (LPS),
which are difficult to measure at the conventional carbon electrodes. For example, thisfilm electrode can measure all DNA bases (including DNA base derivatives e.g., 5‟methylcytosine and 8‟-hydroxy 2‟-deoxyguanosine) more quantitatively than conventional
carbon-based electrodes.3) We used this film to develop a simple DNA methylation
analysis technique. Our methylation detection technique measures the differences between
the oxidation currents of both 5-methylcytosine and cytosine without needs forconventional bisulfite reaction or labeling process.
Moreover, due to their good electrochemical stability and low background current,
the nanocarbon film electrode is suitable for long-term analysis including as the electrodeof an HPLC detector for detecting cerebral gliotransmitter (kynurenic acid) from glial
cells.4) It is recognized that the glial cells plays an important role in gliotransmission(chemical signal), different from neurotransmission (electric signal). It is recently foundthat kynurenic acid is increasing in epilepsy patients but its concentration is quite low(nM). Our electrode enabled to quantitatively determine cerebral kynurenic acid by directoxidation (LOD=20 pM). Our film electrodes therefore contribute for pharmacologicalevaluation of new candidate drug for antiepilepsy.
４９Recently, we developed an electrochemical lipopolysaccharides (LPS) detection
system using the nanocarbon film electrode because the atomically flat surface of thenanocarbon film electrode can be expected to achieve electrochemical measurement of
LPS with low concentration (pg/mL-ng/mL). We achieved superior quantitative
performance for LPS determination in terms of the low detection limit (LOD=0.2 ng/mL)
by using LPS affinity probe consisting zinc-complex, which was superior result to ourprevious reports.5)

Figure 1. Sputtered nanocarbon film electrodes for bioelectroanalysis.

Keywords: Nanocarbon film; lipopolysaccharide (LPS); gliotransmitter; DNA bases
Reference:(a) O. Niwa, J. Jia, Y. Sato, D. Kato, R. Kurita, K. Maruyama, K. Suzuki, S. Hirono, J. Am.
Chem. Soc., 128, 7144 (2006), (b) J. B. Jia, D. Kato, R. Kurita, Y. Sato, K. Maruyama, K.
Suzuki, S. Hirono, T. Ando, O. Niwa, Anal. Chem., 79, 98 (2007), (c) T. Kamata, D. Kato,
H. Ida, O. Niwa, Diamond. Relat. Mater., 49, 25 (2014).
(a) A. Ueda, D. Kato, R. Kurita, T. Kamata, H. Inokuchi, S. Umemura, S. Hirono, O. Niwa,
J. Am. Chem. Soc., 133, 4840 (2011), (b) D. Kato, M. Sumimoto, A. Ueda, S. Hirono, O.
Niwa, Anal. Chem., 84, 10607 (2012).
(a) D. Kato, N. Sekioka, A. Ueda, R. Kurita, S. Hirono, K. Suzuki, O. Niwa, Angew. Chem.
Int. Ed., 47, 6681 (2008), (b) D. Kato, N. Sekioka, A. Ueda, R. Kurita, S. Hirono, K.
Suzuki, O. Niwa, J. Am. Chem. Soc., 130, 3716 (2008), (c) D. Kato, K. Goto, S. Fujii, A.
Takatsu, S. Hirono, O. Niwa, Anal. Chem., 83, 7595 (2011), (d) D. Kato, M. Komoriya, K.
Nakamoto, R. Kurita, S. Hirono, O. Niwa, Anal. Sci., 27, 703 (2011).
S. Yamamura, M. Hoshikawa, D. Kato, H. Saito, N. Suzuki, O. Niwa, M. Okada, Br. J.
Pharmacol., 168 1088 (2013).
(a) D. Kato, S. Iijima, R. Kurita, Y. Sato, J. Jia, S. Yabuki, F. Mizutani, O. Niwa, Biosens.
Bioelectron., 22, 1527 (2007), (b) S. Iijima,. D. Kato, S. Yabuki, O. Niwa, F. Mizutani,
５０Biosens. Bioelectron., 26, 2080 (2011), (c) D. Kato, A. Oda, M. Tanaka, S. Iijima, T.
Kamata, M. Todokoro, Y. Yoshimi, O. Niwa, Electroanalysis, 26, 618 (2014).
Introduction to the author:
Dr. Dai Kato
Biomedical Research Institute, AIST, Japan.
Tel: +81-29-861-16158; Fax: +81-29-861-16177.
E-mail: dai.kato@aist.go.jp
Biography of the authors. Dai Kato received his PhD degree in polymer chemistry from
Kumamoto University in 2003. He is a researcher in AIST. His research interests are in theborderline field between material sciences and analytical chemistry.

５１

KL.05
Odor variation on the car air conditioning evaporator according to thefeeding nutrients
Man-Goo Kim*, Young-Jae Ryu
Dept. of Environmental Science, College of Natural Science, Kangwon National
University, Kangwon, 200-701, Korea,
Abstract: Odor and VOC have much attention in recent years because of their impact on
car interior air quality. As to JD power, the consumer satisfaction included the odor item inthe evaluation of car initial quality study. The odor source is abundant in a car interior but
the air conditioner is the most important source. Automotive air conditioning (HVAC)
system generates an intense odor when it turn on and/or off. One of the major mechanismof odor generation is a metabolism of microorganisms on the surface of the evaporator.
In this study, odor variation of HVAC evaporators were investigated according to feeding
low concentration of nutrients as like rain and leather car seat extracted water. DI water isused as the control nutrient. Each evaporators were supplied each nutrient water threetimes a day. During the 12 weeks, each evaporator were tested and compared of odor
intensities a day and the bench test were one time a week.
Odor intensity was measured by using human olfaction and odor caused components wereanalyzed GC/olfactometer.
The odor intensity of evaporator sprayed DI water was decreased but that of sprayedleather extracted water was increased, which explains that a variability of HVAC
evaporator odor according to feeding nutrients.
Some of odor caused compounds detected old HVAC evaporator were reproduced in theevaporator of sprayed leather extracted water.

５２

The odor generating mechanism in the HVAC system will be discussed

Fig. variation of odor intensity according to feeding nutrients in evaporators of automotive
HVAC system during 12 weeks. (a)DI water, (b) rain and (c) Leather car seat extracted
water
Keywords: odor, air conditioning, car interior, nutrient,
Reference:
1. K.H. Kim, S.H. Kim, Y.R. Jung and M.G. Kim, “Evaluation of malodor for automobile
air conditioner evaporator by using lab-scale test cooling bench”, J. of Chromatography A,
2008, 1204, 72-80.
Introduction to the author:
Professor Dr. Man-Goo Kim
Department of Environmental Science, Kangwon National University, Kangwon, Korea
Tel: +82-33-250-8576; Fax: +82-33-259-5670.
E-mail: mgkim@kangwon.ac.kr
Biography of the authors:
Research interesting
Car cabin interior air quality
Emission of hazardous organics from building materials
Evaluation of Odor active compounds in products

５３

Academic Experience
April 1986
Keio University, Yokohama, Japan-Mar. 1989 Doctor of Engineering, Dept. of Applied Chemistry, March 1989
Thesis: “Studies on analytical methods and behavior of micro organic
compounds in urban suspended particulate matter”
Sept. 1991
Kangwon National University, Kangwon, Korea-Present
Assistant Professor, Associate Professor, Professor in Department ofEnvironmental Science
April 1989
Keio University, Yokohama, Japan-Sept. 1990 Lecturer, Department of Applied chemistrySocial Experience
2008 ~ present Advisor, Korean society of odor research and engineering
Director, Institute of Environmental Research, Kangwon National
2004 ~ 2008
University
Chairman, Analytical division of ecolabelling of healthy building
2005 ~ present
material, Korea air cleaning association2013 ~ present Convenor, ISO/TC 146/SC 6/WG 222015 ~ present Executive vice chairman, The Korean Society of Analytical Science
2015 ~ Present Cochairman, Foundation of Green Future５４

KL.06
Break diffraction-limited barrier with wavelength-modulation
fluorescence-free nanoscopy
Peng Zhanga, Seungah Leeb, Seong Ho Kanga,b,*a

Department of Chemistry, Graduate School, Kyung Hee University, Yongin-si, Gyeonggido 446-701, Republic of Koreab
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446701, Republic of Korea
Abstract: Wavelength-modulation based fluorescence-free nanoscopy was built to breakthe diffraction-limited barrier of optical resolution. This achievement was based on thefact that the diffraction-limited plasmonic nanoparticles could be selective detected bywavelength-modulation and the centers could be measured with precisions much better
than the width of their intensity distributions. Diffraction-limited and color blended
enhanced dark-field (EDF) images of neighboring gold nanoparticles (GNPs), gold
nanorods (GNRs), and silver nanoparticles (SNPs) were modulated at their specificlocalized surface plasmon resonance (LSPR) wavelengths. The point spread functions of
NPs were approximated as symmetric two-dimensional (2D) Gaussian functions and fittedwith least-square algorithm to resolve the central coordinates (x, y). Due to the remarkablesignal-to-noise ratio of EDF illustration system, the Cramér-Rao lower bound (CRLB)
based localization precisions of GNPs, GNRs, and SNPs were measured as 2.5 nm, 5.0 nm,
and 2.9 nm, respectively, which were much better than the width of their intensity
distributions. Even considered the plasmonic coupling effect of particles, these
localization precisions were high enough to achieve super-resolution images. Based on thecorresponding localization precisions, the resolved coordinates were rendered withcircularly symmetric Gaussian blobs and visualized in a reconstructed image. In addition,
the rotation states of GNRs were resolved with polarizer to provide more precise
information of particles. Finally, this novel method was applied in a live single cell andprovided remarkable sub-diffraction limited images of NPs.
Keywords: super-resolution, fluorescence-free, wavelength-modulation
Reference:
1. P. Zhang, S. Lee, H. Yu, N. Fang, S.H. Kang, Sci. Rep., 2015, 5, 11447.
５５

2. B. Huang, H. Babcock, X. Zhuang, Cell, 2010, 143, 1047.
Introduction to the author:
Professor Dr. Seong Ho Kang
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446701, Republic of Korea
Tel: +82-31-201-3349; Fax: +82-31-201-2340
E-mail: shkang@khu.ac.kr
Biography of the authors.
Peng Zhang was born in Shandong, China. He received his B.E. degree from Qilu
University of Technology in 2011. From 2012, he joined in Prof. Seong Ho Kang‟s lab,
Department of Chemistry, Kyung Hee University, as a master and Ph.D. combined student.
His current research focuses on single-molecule/particle detection and super-resolution
microscopy.
Seungah Lee received her B.S., M.S., and Ph.D. degrees in chemistry from Chonbuk
National University of Republic of Korea in 2003, 2006, and 2010, respectively. She iscurrently an Assistant Professor at Kyung Hee University. Her main research interests are
chemical/biological analysis on biochip at single-molecule level using various detection
systems.
Seong Ho Kang received his B.S. and M.S. degree in chemistry from Kyung Hee
University of Republic of Korea in 1987 and 1989. He worked as a senior researcher at the
research center of Hanil Pharmaceutical Co. (present, CJ Pharm. Co.) from 1989 to 1997.
He received his Ph.D. degree in chemistry from the Seoul National University in 1998. He
was a postdoctoral research associate scholar at Iowa State University and Ames
Laboratory US Department of Energy with Dr. Edward S. Yeung from 1998 to 2000, asenior researcher at the SAIT (Samsung Advanced Institute of Science and Technology) in
2000-2002, and a professor of chemistry at Chonbuk National University in 2002-2010.
He is currently a professor and department chairman at Kyung Hee University from 2010.
His research interests include development of various analytical systems, methods, andtechniques based on micro- and nano-separation, single-molecule/particle detection, superresolution microscopy, micro- and nano-fluidics, and various nano-bio fusion technologies.

５６KL.07Development of on-line SFE-SFC system and its application
Yuki Hashi*
Shimadzu (China) Co., Ltd.
Abstract: Sample pretreatment is always one of the key issues in a series of analyticalprocedures. Items such as efficiency of sample extraction, sample throughput,
reproducibility, etc. are evaluated through the analytical validation. On-line sample
pretreatment with an automated chromatographic system is often used for effective andreliable extraction and analysis. Previously we developed on-line pretreatment HPLC
systems with the column switching techniques. The systems showed excellent
performance for analysis of drugs in plasma,1) contaminants in food2) and environment.3)
Supercritical fluid extraction (SFE) has been a commonly used substantial extraction
technique and applied for extraction of bioactive compounds, drugs, lipids, andcontaminants from natural products. SFE is also effective for metabolite analysis in dried
blood spot.4) Compared with other extraction methods, SFE with supercritical carbon
dioxide (SC-CO2) has advantages such as low consumption of organic solvent and low
toxicity. SC-CO2 is also readily removed after use. Supercritical fluid chromatography(SFC) has the mutual advantages with SFE. Compared with HPLC, reduction of theviscosity of mobile phase and the improvement of the diffusion coefficient make better
separation performance in SFC. For the enhancement of productivity and quality ofanalysis, we have developed an on-line SFE-SFC system. Devices like CO2 pump, SFE
unit, BPR (back pressure regulator) were newly designed for the system. Mass
spectrometers can be combined so that the system achieves the maximum performance.
The system was evaluated through the applications such as residual pesticide analysis,
dried blood spot analysis, and cleaning validation.

Fig. 1 On-line SFE-SFC-MS system
Keywords: supercritical fluid extraction, supercritical fluid chromatography, mass
５７

spectrometry
References:
1. S. Kawano, M. Takahashi, T. Hine, E. Yamamoto, N. Asakawa, Rapid Commun. Mass
Spectrom. 2005; 19: 2827.
2. S. Kawano, Y. Hayakawa, Y. Hashi, J.-M. Lin, Anal. Sci. 2015; 31: 445.
3. Y. Hashi, T.-R. Wang, W. Du, J.-M. Lin, Talanta 2008; 74: 986.
4. A. Matsubara, Y. Izumi, S. Nishiumi, M. Suzuki, T. Azuma, E. Fukusaki, T. Bamba, M.
Yoshida, J. Chromatogr. B 2014; 969: 199.
Introduction to the author:
Dr. Yuki Hashi
General Manager, Shimadzu Global COE for Applications & Technical Development,
Shimadzu (China) Co., Ltd., China
Tel: +86-10-8525-2352; Fax: +86-10-8525-2330.
E-mail: y-hashi@shimadzu.co.jp
Biography of the author
Dr. Hashi graduated from Department of Chemistry, Osaka University, Osaka, Japan in
1986. At the same year, he joined Shimadzu Corporation, analytical instrument division todevelop HPLC instruments especially electrochemical detector and column packing
materials for protein analyses. In 1994, he moved to Shimadzu Singapore as an application
manager for chromatography instruments to take care of the customers in South-East Asia
as well as south Asia countries. His major area of interest is development of new
application system using LC, GC, LC-MS, and GC-MS for the enhancement of analysis
efficiency. Based on this research work, he has received Ph.D. degree from ResearchCenter for Eco-Environmental Sciences, The Chinese Academy of Sciences in 2009. He isdeveloping new analytical methods with chromatographic system extensively in thevarious fields such as food safety, environmental, pharmaceutical and life science(genomics and proteomics). Beside his research work, he is also actively providing thelecture of basic instrumentation in university, and contributing his papers to sometechnical conferences.

５８KL.08Novel Solid-Phase Microextraction and Capillary
Electrochromatographic Column Techniques for Pharmaceutical
Analysis
Zilin Chen*, Wenpeng Zhang, Juan Zhang, Zixin Zhang
School of Pharmaceutical Sciences, Wuhan University
Abstract: Mussels attach to solid surfaces such as rocks in the sea. Their adhesion must be
rapid, strong, and tough, or else they will be dislodged and dashed to pieces by the next
incoming wave. The tight adhesion results from the adhesive protein containing
polydopamine secreted by mussels. Inspired by the composition of adhesive proteins inmussels and combined with the chemical self-assembly of multilayer grapheme andmental organic frame materials on chemically resistant plastic micro-tubes such as PEEK
and stainless steel tubes, we have successfully developed several novel solid phase microextraction [1-5] and capillary electrochromatographic columns [6-8] and applied inenvironmental and pharmaceutical analysis. This talk will introduce the preparation,
characterization, validation and application of novel solid-phase microextraction andcapillary electrochromatographic columns based on mussel-inspired polydopaminefunctionalization. Fig. 1 shows schematic of assemblies of multilayer graphene on
chemically resistant plastic micro-tubes. Fig. 2 shows schematic procedure of preparing
GO-PDA-PANI@capillary.

Fig. 1 (Left) Schematic of assemblies of multilayer graphene on chemically resistant
plastic micro-tubes
Fig. 2 (Right) Schematic procedure of preparing GO-PDA-PANI@capillary
Keywords: Solid-phase microextraction, Capillary electrochromatographic column,
５９Polydopamine functionalization,
Reference:
W. Zhang, J. Zhang, T. Bao, W. Zhou, J. Meng, Z. Chen*, Anal. Chem. 2013, 85, 68456854.
W. Zhang, Z. Chen*, J. Chromatogr. A, 2013, 1278, 29-36.
W. Zhang, Z. Zhang, J. Zhang, J. Meng, T. Bao, Z. Chen*, J.Chromatogr. A, 2014,
1351,12-20
J. Zhang, W. Zhang, T. Bao, Z. Chen* J.Chromatogr. A, 2015, 1388, 9-16.
Z. Zhang, W. Zhang, T. Bao, Z. Chen*, J. Chromatogr. A, 2015, 1407, 1-10.
J, Zhang, W. Zhang, T.Bao, Z. Chen*, Analyst, 2014, 139, 242-250
J. Zhang, W. Zhang, T. Bao, Z. Chen*, J. Chromatogr. A, 2014, 1339, 192-199
T. Bao, J. Zhang, W. Zhang, Z. Chen*, J. Chromatogr. A, 2015, 1381,239-246.
Acknowledgment
This work was supported by National Natural Science Foundation of China(No.21375101, 91417301), the Hubei Provincial Scientific Foundation (No 2011CDB475)
and Wuhan Science and Technology Bureau (No: 20140601010057)
Introduction to the author:
Professor Dr. Zilin Chen
School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
Tel: +86-27-68759893; Fax: +86-27-68759850.
E-mail: Chenzl@whu.edu.cn
Dr. Zilin Chen received his Master of Science degree at Wuhan University in 1991,
China and Ph. D of Engineering degree at Tokyo Metropolitan University (TMU), Japan
in 2000. After receiving his Ph. D degree, he worked as postdoctoral fellow of JSPS
(Japan Society for the Promotion of Science) at TMU from 2000 to 2002, as a postdoctoral
researcher at NTT Microsystem Integration Labs, Japan from 2002 to 2003, and as a
research assistant professor at University of Notre Dame, USA from 2004 to 2007. He
joined Wuhan University as a Luojia Chair Professor in Mar. 2007. Now he works as
Luojia Chair Professor, vice dean of School of Pharmaceutical Sciences and director of
Institute of Pharmaceutical Analysis and Drug Screening, Wuhan University.
Dr. Chen received “New Century Prize” from Kanto Branch of Japan Society for
Analytical Chemistry in 2002, and “Shourei Award” from Japan Society for
Chromatographic Sciences in 2003. He was awarded “Zhu Yubi Medical Award” in 2012,
and “Outstanding Contribution Award of Wuhan University” in 2010, 2013 and 2014. He
has had over 100 peer-reviewed papers published in international journals including
６０Analytical Chemistry (ACS), Biosensors & Bioelectronics (Elsevier) and J.Chromatogr. A
etc. In past several years since he came back China in 2007, he has got financial supports
from National Natural Science Foundation of China (NSFC) for 2 key project and 3
normal projects, and other funds from Ministry of Education of China, Hubei Province,
Wuhan Bureau of Science and Technology and Wuhan University. His research interests
include pharmaceutical analysis and drug screening. He organized CJK 2010 at Wuhan.

６１

KL.09Quantitative on-line Concentration and Reaction for Capillary
Electrophoresis with Inkjet Sample Introduction
Ying Rang, Fengming Chen, Hulie Zeng, Hizuru Nakajima, Shungo Kato, Jinmig Lin,
Katsumi Uchiyama*
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences,
Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan
Abstract:
A quantitative sample introduction method based upon inkjet injection was applied tocapillary electrophoresis coupled with 1) stacking and sweeping on-line concentration
techniques, and 2) on-line reaction system. For on-line concentration technique,methylxanthines were used as model compounds for the proof-of-concept of the method.
The volume of injected sample could be easily manipulated by controlling the number ofejected droplets in the injection procedure. Under optimized conditions, a linear
relationship between the ejected droplet number and peak area was obtained when thedroplet number introduced into the capillary was less than 100. Under optimized
quantitative on-line concentration conditions, the limit of detection for theobromine,
caffeine and theophylline were 1.0, 2.0 and 1.0 μM, respectively. The inkjet injection
system was evaluated by comparing it with conventional injection methods. The
electropherogram of the inkjet injection mode was the same as that for hydrodynamic
injection mode, and no sample discrimination was observed compared with theelectrokinetic injection mode. The established method was applied to the determination of
methylxanthines in bottled green tea. The recoveries of theobromine, caffeine andtheophylline were 94.1, 110.6 and 86.8%, respectively. We conclude that proposed method
can be used for quantitative concentration for capillary electrophoresis, thus resulting in an
improved accuracy.
For on-line reaction technique, NBD-F and amino acids were alternately introduced as
small plugs followed by the reaction and separation.
Keywords: quantitative; volume-control injection; capillary electrophoresis; inkjet
technique; on-line concentration, on-line reaction

６２

Reference:
1. Ying Rang, Hulie Zeng, Hizuru Nakajima, Shungo Kato, Katsumi Uchiyama, Journalof Separation Sciences, 2015(in press).
2. Fengming Chen, Ying Rang, Ying Weng, Luyao Lin, Hulie Zeng, Hizuru Nakajima, JinMing Lin and Katsumi Uchiyama, Analyst, 2015, 140, 3953-3959.
3. Jianmin Yang, Daisuke Katagiri, Sifeng Mao, Hulie Zeng, Hizuru Nakajima, Katsumi
Uchiyama, The Royal Society of Chemistry Advances, 2015, 5, 7297–7303
4. Fengming Chen , Sifeng Mao , Hulie Zeng , Shuhua Xue , Jianmin Yang , Hizuru
Nakajima , Jin-Ming Lin, and Katsumi Uchiyama, Analytical Chemistry, 2013, 85 (15), pp
7413–7418
5. Ying Weng, Hulie Zeng, Yuri Nakagawa, Saori Ikeda, Fengming Chen, Hizuru
Nakajima, Katsumi Uchiyama, Chromatography, 2014, Vol.34, No.1, pp.33-40
6. Chen Luo, Yuan Ma, Haifang Li, Fengming Chen, Katsumi Uchiyama and Jin-Ming Lin,
Journal of Mass Spectrometry, 48(3), 321-328 (2013)
7. Fengming Chen, Yandong Zhang, Yuri Nakagawa, Hulie Zeng, Chen Luo, Hizuru
Nakajima, Katsumi Uchiyama, Jin-Ming Lin, Talanta, 107, 111-117, (2013)
8. Hulie Zeng , Ying Weng , Saori Ikeda , Yuri Nakagawa , Hizuru Nakajima , andKatsumi Uchiyama, Analytical Chemistry, 2012, 84(24), pp 10537–10542
9. Hulie Zeng, Yoriko Inoue, Kosuke Moritani, Moeka Nishiwaki, Hizuru Nakajima,
Katsumi Uchiyama, Sensors and Acturators B: Chemical, 168, pp446~452 (2012)
10. Fengming Chen, Zhen Lin, Yongzan Zheng, Hulie Zeng, Hizuru Nakajima, Katsumi
Uchiyama, Jin-Ming Lin, Analytica Chimica Acta, 739, 77~82 (2012).
Introduction to the author:
Professor Dr. Katsumi Uchiyama
Department of Applied Chemistry, Faculty of Urban Environmental Sciences, Tokyo
Metropolitan University, Tokyo, Japan
Tel: +81-426 77 2835; Fax: +81-426 77 2821.
E-mail: uchiyama-katsumi@tmu.ac.jp
Biography of the authors.
Katsumi Uchiyama is Professor of Tokyo Metropolitan University. He received PhD from
Hoshi University, Japan in 1982. 1995, he was an Associate Professor of Tokyo
Metropolitan University; and 1997, he was a Visiting Scholar of Mt. Sinai School of
Medicine (NY, USA); 2006 to now, he is a Professor of Tokyo Metropolitan University.
He was awarded a “OTANI Prize” at 1983, most excellent paper in “BUNSEKIKAGAKU” at the year 2001, 2006. In 2013, he received a "CSJ award for Chemical
Eductaion". From 1981to 1994, he devoted to the study on “The application of
Photoacoustic spectroscopy to biological materials”. From 1995 to now, he is interested in
６３

the micro-TAS, on chip micro gas chromatography, analytical application of micro droplet
and fabrication of micro chemical devices. He is member of ACS, CSJ (head of ChemChem-Club for young expecting scientists in 2006~2013), JSAC (vice president in2013~2014) and JSP.

６４

KL.10Discrimination technique using fragmentless ionization mass
spectrometry and multivariate analysis for food analysis.
Takahisa Tsugoshi*
National Institute of Advanced Industrial Science and Technology (AIST)
Abstract:

Soft ionization techniques especially to be remarkable at nofragmentation during ionization have an advantage to be obtained only (quasi-)molecularion peaks in the mass spectrum. There is less chemical reaction during the ionization, sothat matrix effect for peak intensity does exist rarely. It means multivariate analysis can beapplied easily, that is no need to prepare condition to calculate. According to the points
aimed at, we propose to call “fragmentless ionization” for a kind of soft ionizations, e.g.,
ion attachment ionization and photo-ionization.
- Coffee beans We examined a method to discriminate Coffea Arabica (Arabica) and Coffea
canephora (Robusta). In order to explore the respective biomarkers, ion attachment
ionization mass spectrometer (IA-MS) equipped with a direct inlet probe (DIP) was used.
It was possible to measure the solid samples directly by DIP. Furthermore, in IA ionization
mass spectra, only quasi-molecular ion peaks are obtained, so that the mass spectra
showed the characteristic pattern, which reflected the states in the sample. Therefore, easy
screening analysis was possible. By using DIP/IA-MS, we measured nine kinds of roasted
coffee beans. As a result, the peaks estimated to be Kahweol palmitate and 16-Omethylcafestol palmitate were detected from Arabica and Robusta, respectively. In
addition, this method offers estimation of the mixed ratio of the Arabica and Robusta in
not only roasted coffee beans milled but extract with and without heat sterilization.
- Snack Confectionery Samples were 10 kinds of commercial snack confectionery on the Japan‟s market,
so-called as chips. Each sample was crushed, and the powdered specimen was measured.
The residual analysis which is one of the multivariate analysis was applied for thecomparison. By the comparison between an ordinary snack and infant one that avoids any
oils, peaks for palmitic acid, oleic acid, triolein, etc. were detected only from ordinary one
as shown in Figure.
６５

Fig. Residual analysis result between mass spectra of ordinary and infant snacks.
Keywords: coffee, snack, fragmentless ionization, ion attachment ionization, mass
spectrometry, residual analysis
Reference:
1. Y. Mishima, H. Maruyama,K. Hinno, T. Tsugoshi, N. Saito, Y. Nishimoto, T. Mitsui, 60，
409（2011）(in Japanese).
2. R. Okumura, N. Kumata, T. Tsugoshi, Y. Mishima, H. Koizumi, Bunseki kagaku, inpress (in Japanese)
Introduction to the author:
Dr. Takahisa TSUGOSHI
National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Tel: +81-29-861-4997; Fax: +81-3-6745-1955.
E-mail: tsugoshi.takahisa@aist.go.jp
Biography of the author.
Dr. Takahisa TSUGOSHI received his BA degree in 1988 and received a PhD
degree in analytical chemistry at Tokyo University of Science. He had studied and worked
in Tokyo University of Science for 12 years. He was a senior research scientist of
Advanced Sintering Technology Group, Advanced Manufacturing Research Institute,
AIST, and is currently a senior research scientist of National Institute of Advanced
Industrial Science and Technology (AIST). He is also a member of several committees andan ex-chairperson of Young Analytical Chemist Association, the Japan Society for
Analytical Chemistry (JSAC). He won the Research Award 2007 of the Mass
Spectrometry Society of Japan, New Century Research Award 2009 of JSAC, Advanced
Analytical Technique Award (CERI Award) 2011 of JSAC, etc.

６６KL.11Introduction of 2016 CJK Symposium in China
Chunhua Ma
College of Tea and Food Science, Wuyi University, Wuyi Rd. 16, Wuyishan 354300,
China. E-mail:chma@wuyiu.edu.cn

Introduction for 2016 CJK symposium in China.

６７

KL.12
Fast Analysis by Microextraction Coupled to Ambient Mass
Spectrometry
Xin Wang, Xianjiang Li, Yu Bai, Huwei Liu*
Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering,
Peking University, Beijing 100871, China
Abstract: The determination of trace residues and contaminants in complicated matrix
often requires extensive sample preparation prior to instrumental analysis. Usually,
sample preparation is the bottleneck in a whole analytical procedure, and minimized
preparation steps are highly desired to reduce both time and sources of error. On the other
hand, most analytical methods rely on the separation by liquid chromatography (LC) or
gas chromatography (GC), which make the entire method complicated and timeconsuming. Ambient mass spectrometry (AMS) has been frequently used for the rapid
determination or screening of analytes, even without the need of chromatography
separation in some cases.
In this presentation, we report our initial efforts to combine effective microextraction with
AMS for high throughput analysis of targeted compounds in complex samples, including
rapid screening for synthetic antidiabetic drug adulteration in herbal dietary supplements,
rapid analysis of multiple phytohormones in fruit juice, fast analysis of triazine herbicides
in environmental samples and four Sudan dyes in food samples.
Keywords: Fast analysis, microextraction, ambient mass spectrometry
Reference:
1. Zhigui Zhou, Jialing Zhang, Wei Zhang, Yu Bai and Huwei Liu, Analyst, 2011, 136:
2613- 2618
2. Yu Bai, Jialing Zhang, Yu Bai and Huwei Liu, Anal. Bioanal. Chem, 2012, 403: 23072314
3. Xianjiang Li, Jiawei Xing, Cuilan Chang, Xin Wang, Xiuping Yan, Yu Bai and Huwei
Liu, J. Sep. Sci., 2014, 37: 1489-1495
4. Xin Wang, Xianjiang Li, Ze Li, Yiding Zhang, Yu Bai and Huwei Liu, Anal. Chem.,
2014, 86: 4739-4747
5. Ze Li, Yi-Wei Zhang, Yi-Ding Zhang, Yu Bai, Hu-Wei Liu，Anal Methods, 2015, 7:
６８

86-90
6. Xin Wang, Xianjiang Li, Yu Bai and Huwei Liu, Chem Comm, 2015, 51: 4615-4618
Introduction to the author:
Professor Dr. Huwei Liu
Department of Chemistry, Peking University, Beijing 100871, China
Tel: +86-10-62754976; Fax: +86-10-62751708.
E-mail: hwliu@pku.edu.cn
Biography of the authors.
Dr. Huwei Liu graduated with a BA degree in 1982, and PhD in 1990 from Beijing
Institute of Technology (BIT), China, then he joined Peking University. He is currently amember of Chinese Chemical Society and a vice president of Beijing ChromatographySociety. He also serves as an associate editor of Journal of Separation Science and Journalof Instrumental Analysis (in Chinese), an advisory/editorial board member for Analytical
Chemistry, Analytical and Bioanalytical Chemistry, Chinese Journal of Chromatography,
and other nine Chinese journals. Dr. Liu‟s research work focuses bioanalysis and detection,
including fundamentals and applications of chromatographic and electrophoretic
separation techniques, as well as hyphenated methods such as LC/MS and CE/MS. The
application areas cover pharmaceutical analysis, plan hormone detection, proteomics andlipidomics analysis. He is the author and co-author of more than 180 original research
papers, several reviews and chapter contributions of the edited books.
Recent Publications:[1] C. Chang, X. Qi, J. Zhang, Y. Qiu, X. Li, X. Wang, Y. Bai, J. Sun and H. Liu,
Facile synthesis of magnetic homochiral metal-organic frameworks for "enantioselective
fishing'', Chem Comm, 2015, 51, 3566 – 3569
[2] X. Wang, X. Li, Y. Bai and H. Liu, Just dip it: Just dip it: online coupling of “Dip-It”
polymer monolith microextraction with plasma assisted laser desorption ionization mass
spectrometry, Chem Comm, 2015, 51, 4615-4618[3] Z. Zhang, T. Bo, Y. Bai, M. Ye, .R. An, F. Cheng, H. Liu, Quadrupole time-of-flightmass spectrometry: powerful tool for demystifying traditional Chinese medicine, TrAC, inpress[4] M. Li, L. Yang, Y. Bai, H. Liu, Analytical Methods in Lipidomics and Their
Applications, Anal. Chem. 2014, 86, 161−175[5] X. Wang, X. Li, Z. Li, Y. Zhang, Y. Bai and H. Liu, Online Coupling of In-tube Solid
Phase Microextraction with Direct Analysis in Real Time Mass Spectrometry for Rapid
Determination of Triazine Herbicides by Using Polymer Monolith Incorporated with
Single-Wall Carbon Nanotubes, Anal. Chem., 2014, 86, 4739−4747.
６９

[6] Y. Liu, J. Zhang, H. Nie, C. Dong, Z. Li, Z. Zheng, Y. Bai, H. Liu and J. Zhao, Studyon Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using
Easy Ambient Sonic-Spray Ionization Mass Spectrometry, Anal. Chem., 2014, 86,: 70967102C.
[7] Y. Zhang, X. Li, H. Nie, L. Yang, Z. Li, Y. Bai, L. Niu, D. Song, H. Liu, An Interface
for On-line Coupling of Surface Plasmon Resonance to Direct Analysis in Real Time Mass
Spectrometry, Anal Chem, DOI:10.1021/acs.analchem.5b01272, in press

７０KL.13Detection of Benzo[a]pyrene-induced Breast Cancer Stem Cells Using
Quantum Dot-based High-content Assay
Yumi Shim, Joon Myong Song*
College of Pharmacy, Seoul National University, Seoul 151-742, Korea
Abstract: Cancer stem cell (CSC) hypothesis has been reported by many studies before.
Due to drug and radiation resistance inherent to CSC, CSC has received great attention in
the field of cancer therapy. In this study, it was found that breast CSCs were produced
from MCF-7 cells by benzo[a]pyrene (BP)-induced mutation. Breast CSCs were obtainedusing magnetic bead-based sorting from MCF-7 cells and detected through high-content
monitoring of three different markers CD44, CD24 and aldehyde dehydrogenase1(ALDH1)
using a hypermulticolor cellular imaging system based on acousto-optical tunable filter(AOTF) . The BP-induced mutation was quantitatively observed via absorption spectra of
BPDE-DNA adducts that were formed in MCF-7 cells by the treatment of MCF-7 cellswith BP. MCF-7 cells were treated with BP at different concentration of 0.2μM, 2μM,
5μM and 10μM for 24hr. The resultant CSCs in the entire MCF-7 cells were determined tobe 0.35±0.032%, 0.45±0.038%, 0.55±0.075%, 1.02±0.28% and 1.19±0.27% in control,
0.2μM, 2μM, 5μM and 10μM respectively. Until now, FACS has been used for thedetection of CSCs as general tool. In this work, it is verified that the present multicolor
cellular imaging is very advantageous for the detection of CSCs because spectral overlap
among probes of CSC biomarkers can be eliminated and diagnostic accuracy can begreatly improved, compared with the conventional FACS.
Keywords: High-content Imaging, Cancer Stem Cell, Quantum Dot
Reference:
1. M. Kakarala and M. S. Wicha, Journal of Clinical Oncology, 2008, 26, 2813-2820.
2. Y. Shim and J. M. Song, Chemical Communications, 2015, 51, 2118-2121.
Introduction to the author:
Professor Dr. Joon Myong SongDepartment of Pharmacy, Seoul University, Seoul 151-742, Korea
Tel: +82-2-880-7841; Fax: +82-2-880-9178.
７１

E-mail: jmsong@snu.ac.kr
Biography of the authors.
Joon Myong Song received his PhD in 1997 at Kyushu University, in Japan. He worked asa postdoctoral research fellow from 1998 to 2004 at Iowa State University, Brookhaven
National Laboratory, and Oak Ridge National Laboratory in United States. At present he isa professor and head of Department of Pharmach at College of Pharmacy School, Seoul
National University in South Korea. His research area includes multifunctional
nanoparticle for diagnosis and therapy and high-content cell-based drug screening anddiagnosis using hyper-multicolor cellular imaging. He has published 84 peer reviewed
papers in the top journals, 7 book chapters, and 10 patents.

７２

KL.14
Determination of copper content in processed food by ICPJae Sung Kim, Yoon Jung Yoo, Byeong Hyeon So, Kwang Sik Jung, Hyun Mi Koo, Ji
Won Lee, Jang-Hyuk Ahn*, Sang Rag Lee, Young Ryul Kim
KOTITI Testing & Research Institute, Seongnam 462-807 Republic of Korea
Introduction and Results
Quantitative analytical method for copper in processed food was established by using
rapid microwave digestion and ICP(Inductively Coupled Plasma). Copper content
determination was validated for the sample pre-treatment and instrumental process. Since
the method could be used to conclude whether copper chlorophyll was added illegally or
not although the colorant is permitted in some processed foods in Korea, establishment ofcopper content method in some food with complex matrix was needed. Key point of thesample pre-treatment was to prevent boil over in the microwave vessel using proper
combination of pre-heat, cooling and acid concentration. Calibration curve for ICP
instrumental analysis showed good linearity with 0.9999 regression coefficients and theresult of recovery test showed within 90∼110 %.

Keywords: Copper chlorophyll, Copper(Cu), ICP(Inductively Coupled Plasma)
Reference(1) Y Şahan, F Basoglu, S Gücer, Food Chemistry 2007, 105, 1, 395-399.
(2) Stephen P. McGrath,Caroline H. Cunliffe, J. Science of Food and Agricure. 2006, 36,
794-978.
(3) Jasmina Djedjibegovic, Thorjørn Larssen, Armin Skrbo, Food Chemistry, 2012, 131, 2,
469-476.
Biography
Dr. Jang-Hyuk Ahn completed his doctorate in Biological Science at the Department of
Biological Science, Korea Advanced Institute of Science and Technology(KAIST) after
master course for Food Science & Technology, Korea University. After 1993, he
performed research in Lotte R&D Center, MFDS(Ministry of Food & Drug Safety),
７３

Namyang Dairy R&D Institute as a Food safety center leader. Currently, He is leading
Water & Food Analysis Division, KOTITI Testing & Research Institute. His research is
focused on analytical science and published 42 articles. He is participated in IDF KOREA(International Dairy Federation) and KOLAS (Korea Laboratory Accredited
Scheme) as a professional.
Acknowledgement
This research was supported by a grant (15162MFDS017) from Ministry of Food andDrug Safety in 2015.

７４

KL.15Micro ion extractor for transferring inorganic and organic trace anionsfrom a single drop of whole blood
Kei Toda*, Hiroka Nishiyama, Yukihide Nakamura, Yurika Imoto, Shin-Ichi Ohira
Department of Chemistry, Kumamoto University, 2-39-1, Kurokami, Kumamoto 860-8555,
Japan
Abstract:
Analysis of contents in biological samples, such as blood, saliva and urea etc., is important
to investigate disease and health conditions. Especially blood samples contain a lot ofchemicals. If the analysis can be frequently performed, tracing chemicals and metabolic
dynamics will be monitored. Normally the blood analysis is performed after pretreatment
to obtain serum. The pretreatment requires mL order of blood sample. In this work, we
aimed to analyze trace anions from a single drop of whole blood without any otherpretreatment, and micro ion extractor (MIE) device was developed.
The device for the extraction comprised of a 16-μL sample cavity, anion permeable
membrane, acceptor solution layer, ion exchange membrane, isolator solution layer, andanode plate [1]. Whole blood sample was obtained by earlobe prick with a lancet. The
exuded blood was aspirated (25 μL) by a micropipette and was immediately transferred tothe sample well of the MIE device and covered with an anode electrode rod forelectroextraction. DC voltage was applied for 1–5 min to transfer anions to the acceptor
layer which was directly connected to ion chromatograph (IC) sample loop. Then theanalytes of interest were determined by conductivity-based IC or IC-MS.
While iodide, thiocyanate and perchlorate were quantitatively extracted from aqueous
standard solution and also from saliva but with harder condition, it was very hard to
extract from blood samples. However, by optimizing the condition, 30-40% of recoveries
were obtained for these anions in blood. Relative standard deviations of recovery were
within 10% for the each anion and the method was successfully applied to the real blood
analysis. Interestingly thiocyanate concentration was higher in blood from tobaccosmokers. Also hourly sampling/analysis was repeated to obtain diurnal variations ofthiocyanate and iodide. Particularly iodide concentration showed significant variations
depending on the food intake.
In addition to the inorganic anions, some organic compounds were tried to analyze inthe same method. For examples, medicine such as loxoprofen‟s pKa is 4.2 and supposed to
７５

be anion in the neutral medium. However transfer of such organic anions from blood wasvery hard and extraction efficiency was in several %. This problem was solved byinvestigating a new interface between the blood and acceptor. Namely a supported liquid
membrane was examined to transfer the organic anions. Also indirect contact of theelectrode was investigated. Finally we succeeded to detect the medicine from a single dropof whole blood.
Keywords: single drop of whole blood, micro ion extractor, thiocyanate, iodide, medicine
Reference:
1. K. Toda, Y. Nakamura, H. Nishiyama, S. Ohira, P.K. Dasgupta, Anal. Chem. 2015, 87,
6483–6486.

Introduction to the author:
Professor Dr. Kei Toda
Department of Chemistry, Kumamoto University, 2-39-1, Kurokami, Kumamoto 860-8555,
Japan
Tel: +81-96-342-3389; Fax: +81-86-342-3389.
E-mail: todakei@sci.kumamoto-u.ac.jp
Biography of the authors.
Dr. Toda graduated from Kumamoto University in 1985 receiving master degree. He
joined in academics of Kumamoto University in 1994 and is professor since 2008. He has
been working on thin film gas sensors, micro fluidic devices for gas analysis (micro Gas
Analysis System: µGAS) and biological analysis. Environmental chemistry andatmospheric chemistry are also his interest and these developed devices are useful tools toinvestigate the scientific issues as well as the advanced commercial instruments.

７６KL.16

Lysine surface modified Fe3O4@SiO2@TiO2 microspheres-based
preconcentration and photocatalysis for in situ selective determination of
nanomolar dissolved organic and inorganic phosphorus in seawater
Shunxing Li*, Wenjie Liang
College of Chemistry and Environment & Fujian Province Key Laboratory of Modern
Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou
363000, P. R. China
Abstract: Because of the lack of well-established methods, dissolved organic phosphorus(DOP) are rarely measured.1 The sample pretreatment methods for dissolved inorganic
phosphorus (DIP) monitoring are time-consuming, inadequate for maintaining original
sample composition, show contradictory results and in some cases use preservation
chemicals that can interfere with the analysis.2 Furthermore, the processes such as
precipitation, hydrolysis, and microbial uptake and release during storage, can mean thatthe DIP concentrations at the time of analysis may not be representative of those within
the natural seawater at the time of sampling.2 In many areas of the remote ocean, rapid
biological uptake of P from sunlit euphotic zone, often driving P concentrations down tonanomolar levels.3 The concentrations of DIP and DOP in coastal waters reveal a highlevel of both spatial and temporal variability.4 Therefore, it is desirable to utilize in situ
sampling methods, which avoid the majority of these issues, to accurately selective
measure the concentrations of DIP and DOP in coastal water and ocean surface water forunderstanding eutrophication processes and P biogeochemical cycling.
A new method for in situ selective spectrophotometric determination of nanomolar
dissolved DIP and DOP in seawaters is offered for the first time, which is based on solidphase microextraction of DIP and photocatalysis induced species transformation of DOP
into DIP. The procedure was shown in Figure 1.

７７

Figure 1 Procedure for in situ selective spectrophotometric determination of dissolved DIP
and DOP
The zeta potential of nanosized metal oxides could be adjusted by surface
modification (seen in Figure 2), which is reported by us for the first time. After surface
modification with lysine, the zeta potential vs pH curve for FST could be reversed andthen 98.1% of DIP anions in the seawater could be specifically adsorbed by electric
attraction onto FST-Lys (1.2 mg/mL) at the pH value of natural seawater (8.1-8.3) after
only stirring for 30 min. The adsorbed DIP could be eluted with hydrochloric acid solution(pH 4.0) for electric repulsion. Therefore, acid hydrolysis of DOP could be inhibited andits influence on the determination of DIP could be avoided.

Figure 2 Zeta potential of FST-Lys and FST in different pH
This method is high sensitivity, rapid, simple, green sample pretreatment, wide linear
range (3.0-1200 nmol/L), high sensitivity (detection limit of 1.42 nmol/L), and appropriate
for field monitoring.
７８

Keywords: Phosphorus
nanomaterials

speciation,

preconcentration,

photocatalysis,

functional

Reference:
1. Aminot, A.; Kérouel, R., Mar. Chem. 2001, 76,113
2. Panther,J.G.; et al., Environ. Sci. Technol. 2010, 44, 9419
3. Ma, J.; et al., Mar. Chem. 2008, 111, 151
4. Paytan, A.; McLaughlin, K., Chem. Rev. 2007, 107, 563
Introduction to the author:
Prof. Dr. Shunxing Li
College of Chemistry and Environment & Fujian Province Key Laboratory of Modern
Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou
363000, P. R. China
Tel: +86-596-2591395. Fax: +86-596-2591395.
E-mail: lishunxing@fjzs.edu.cn; shunxing_li@aliyun.com
Biography of the authors.
Shunxing Li received his Ph.D. in Environmental Science from Wuhan University in 2003.
He now is a professor and vice-president of the Minnna Normal University. His researchinterests cover metal speciation analysis, bioavailability assessment, and biogeochemical
cycle, funtional nanomaterials and its application for pollutant removal.
Wenjie Liang is now a master in College of Chemistry and Environment, Minnan Normal
University, majoring in funtional nanomaterials and its application for environmental
analysis.

７９KL.17

The research of sample preparation method based on metal organic
frameworks

Gongke Li*, Yuling Hu, Zelin Huang, Haixian Lian, Yichun Huang,Suling Zhang, Yifeng
Fan, Zhuomin Zhang
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou,
510275, P.R.China
Abstract: Porous metal-organic frameworks (MOFs) are highly ordered crystalline
materials prepared by the self-assembly of metal ions with organic linkers to yield low
density network structures of diverse topology. Their unique characteristics make MOFs
promising for diverse applications in analytical chemistry, for instance, as sorbents forsampling, solid-phase extraction, solid-phase microextraction, and as stationary phases forchromatography. In this paper, the sample preparation methods based on metal organic
frameworks were researched.
A facile and efficient strategy about the synthesis of a novel kind of hybrid magnetic metalorganic framework MOF-5 via chemical bonding assembly was reported. The assynthesized materials combine the favorable attributes of both magnetic characteristics
and high porosity, making them excellent candidates for magnetic separation andenrichment of trace analytes. Their potential applications as magnetic adsorbents were
explored by preconcentrating polycyclic aromatic hydrocarbons (PAHs) and gibberellicacid (GAs) from environmental, food and plant samples prior to gas chromatography-mass
spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LCMS/MS), respectively. The results showed that the magnetic MOF-5 exhibited superior
enrichment capacity for both of these nonpolar and polar analytes. In-situ solvothermal
growth of metal-organic framework–5 supported on porous copper foam for noninvasive
sampling of plant volatile sulfides was also studied.
A simple and mild multilayer interparticle linking strategy was proposed to fabricate anovel hybrid MOF-199 enrichment coating. Strong chemical interparticle linkages
throughout the coating improved the durability and reproducibility of hybrid MOF-199
coating dramatically. This coating performed a significant extraction superiority of
８０ethylene over commonly-used commercial coatings, attributed to the multiple interactions
including „molecular sieving effect‟, hydrogen bonding, open metal site interaction and ππ affinity. The hybridization of multiwalled carbon nanotubes with MOF-199 further
improved the enrichment capability and also acted as a hydrophobic „shield‟ to prevent the
open metal sites of MOF-199 from being occupied by water molecules. It was
successfully applied for the noninvasive analysis of trace ethylene from fruit samples
coupling with gas chromatography. The detection limit was 0.016 μg/L. MOF199/graphite oxide hybrid composites coated solid-phase microextraction fiber coupledwith gas chromatography for determination of organochlorine pesticides from complicatedsamples was also developed.
A facile method for in-situ fabrication of the Fe3+-1,3,5-benzenetricarboxylic acid (BTC)
metal-organic coordination polymer gel in capillary, which was efficiently applied to theonline enrichment of trace polycyclic aromatic hydrocarbons (PAHs) in environmental
water and amphetamines drugs in urine. Water stable metal-organic framework packed
microcolumn for online sorptive extraction and direct analysis of naproxen and its
metabolite from urine sample was also discussed at the same time.
Keywords: Sample preparation; metal organic frameworks, trace analysis.
Acknowledgements: This work was supported by the Special Funds of the National
Natural Science Foundation of China (No.21127008), the National Natural Science
Foundation of China (Nos. 91232703 and 21475153) and Specialized Research Fund forthe Doctoral Program of Higher Education (No.20120171110001), respectively.
Introduction to Professor Dr. Gongke Li
Institute of Analytical Chemistry, School of Chemistry & Chemical Engineering
Sun Yat-sen University, 135 Xingang Xi Road, Guangzhou 510275, P.R.China
Tel/FAX: 86-20-84110922，84115107; Email: cesgkl@mail.sysu.edu.cn
Prof. Li obtained her BSc(1986) in Chemistry, MSc(1989) and PhD(1992) degrees inAnalytical Chemistry from Sun Yat-sen University. She became a professor since 2000.
She worked in the Tokai University of Japan as a visiting scholar from 1994 to 1996. She
was the director of the Institute of Analytical Sciences of School of Chemistry andChemical Engineering since 2001.With expertise in chromatography analysis and spectral
analysis, her major research interest is focused on the developments of modern sample
preparation techniques and analytical techniques for trace analysis of complex systems,
such as food, biological and environmental samples. She also studies both analytical- andpreparative-scale separations methods for natural products. She has published more than
８１280 peer-refereed journal articles, one monograph and twenty five patents for invention.
She is currently a Senior Editor of Journal of Separation Science and a member ofeditorial board of twelve academic journals including Journal of Chromatography A, and amember of the chemical teaching Advisory Committee for Colleges and Universities ofthe Ministry of Education. 2 Science and Technology Awards and 2 honor prizes havebeen granted to her from the national and provincial governments.
Selected Publications[1]. Jialiang Pan, Shu Jia, Gongke Li, Yuling Hu. Organic building block based
microporous network SNW-1 coating fabricated bymultilayer interbridging strategy for efficient enrichment of trace volatiles. Analytical
Chemistry, 2015, 87(6):3373-3381.
[2] Yuling Hu, Haixian Lian, liangjun Zhou, Gongke Li. In situ solvothermal growth ofmetal-organic framework-5 supported on
porous copper foam for noninvasive sampling of plant volatile sulfides. Analytical
Chemistry, 2015, 87:406-412.
[3] Kaige Zhang, Su Yao, Gongke Li, Yuling Hu. One-step sonoelectrochemical
fabrication of gold nanoparticle/carbon nanosheet
hybrids for efficient surface-enhanced Raman scattering. Nanoscale, 2015, 7:2659-2666.
[4] Runkun Zhang, Yufei Hu, Gongke Li. Development of a cyclic system forchemiluminescence detection. Analytical Chemistry,
2014, 86: 6080-6087.
[5] Yuling Hu, Jia Liao, Dongmei Wang, Gongke Li . Fabrication of gold nanoparticles embedded metal-organic framework for highly
sensitive surface-enhanced Raman scattering detection. Analytical Chemistry, 2014,
86:3955-3963.
[6] Zhuomin Zhang, Yichun Huang, Weiwei Ding, Gongke Li. Multilayer interparticle
linking hybrid MOF-199 for non-invasive enrichment and analysis of plant hormone
ethylene, Analytical Chemistry, 2014, 86: 3533-3540.
[7] Yuling Hu, Zelin Huang, Gongke Li. Chemical bonding approach for fabrication of
hybrid magnetic metal-organic framework-5: high efficient adsorbents for magnetic
enrichment of trace analytes. Analytical Chemistry, 2013，85（14）: 6885-6893.
[8] Yuling Hu, Jialiang Pan, Kaige Zhang, Haixian Lian, Gongke Li. Novel applicationof molecularly imprinted polymer in sample preparation. Trends in Analytical Chemistry,
2013, 43:37-52.
[9] Ting Zhou, Xiaohua Xiao, Gongke Li. Hybrid field-assisted solid-liquid-solid
dispersive extraction for the determination of organochlorine pesticides in tobacco withgas chromatography. Analytical Chemistry, 2012, 84(1):420-427.
[10] Yuling Hu, Yifeng Fan, Zelin Huang, Chaoyong Song, Gongke Li. In situ fabrication
８２

of metal–organic hybrid gels in a capillary for online enrichment of trace analytes inaqueous samples. Chemical Communication, 2012, 48:3966-3968.
[11] Ting Zhou, Xiaohua Xiao, Gongke Li. Microwave accelerated selective soxhlet
extraction for the determination of organophosphorus and carbamate pesticides in ginseng
with gas chromatography/mass spectrometry. Analytical Chemistry, 2012, 84(13):58165822..
[12]. Suling Zhang, Zhuo Du, Gongke Li. Layer-by-layer fabrication of chemical-bonded
graphene coating for solid-phase microextraction. Analytical Chemistry, 2011, 83(19):7531-7541.
[13] Zhe Jiao, Xiaoxi Si, Gongke Li, Zhuomin Zhang, Xinping Xu.Unintended
Compositional Changes in Transgenic rice seeds (Oryza sativa L.) studied by spectral andchromatographic analysis coupled with chemometrics methods, Journal of Agriculturaland Food Chemistry，2010,58:1746-1754.
[14] Yi Zhang, Ruijin Liu, Yuling Hu, Gongke Li. Microwave heating in preparation ofmagnetic molecularly imprinted polymer beads for trace triazines analysis in complicatedsamples. Analytical Chemistry, 2009, 81(3):967-976.

８３

KL.18
Electrochemical and optical monitoring of enzyme reaction on compact
disc-type microchip
Toshihiko Imato
Departament of Applied Chemistry, Graduate School of Engineering, Kyushu University,
744 Motooka, Nishi-ku, Fukuoka, 819-0395 Japan (E-mail address: imato@cstf.kyushuu.ac.jp)
Abstract: A flow-based analysis on a compact disc (CD)-type microchip has beenrecognized as one of the promising analytical methods, where sample solutions, reagent
solutions in reservoirs are delivered to a detector cell through microchannels by using acentrifugal force due to rotation of the microchip. Indeed some analytical instruments
based on the CD-type microchips have been launched. The analytical method using theCD-type microchip does not need any mechanical pumping system, neither connectors
and switching valves for changing flow direction, compared to a conventional flow-baseanalytical method using a mechanical pumping system or an electro-osmotic flow system.
One of the advantages of the analytical method based on the CD-type microchip is that theflow sequence can be controlled by selecting rotation speed, because the critical rotation
speed, at which a solution in a reservoir is started to flow, is dependent of the poison thereservoirs on the CD-type microchip.
In our previous work [1,2], we have attempted to use a CD-type microchip for thedetermination of nonionic surfactant, alkylphenol polyethoxylates (APE) by using an
immunoassay method, where the magnetic microbeads immobilized with an anti-APE
antibody on their surface were placed at the detection position of the CD type microchip.
The chemiluminescence detection was used for this assay. A surface plasmon resonance(SPR) sensor is also a promising detector for the immunoassay on the CD-type microchip.
Indeed we have attempted to fabricate an SPR sensor on a CD-type microchip and applied
to the immunoassay of IgA [3]. Recently we have assembled an electrochemical detector
or carbon paste electrode detector onto the CD-type microchip and applied it to thedetermination of glucose based on an enzyme reaction with glucose oxidase [4].
In this lecture, we will review our recent works on the flow analysis based on the CDtype microchip including the work on an optical monitoring an enzyme reaction. To
monitor an enzyme reaction of horseradish peroxidase (HRP), we have assembled afluorescent detection system on the rotation turn table for the CD-type microchip, as
８４

shown in Fig. 1. In this case, Amplex red and hydrogen peroxide were used as thesubstrates for HRP and a fluorescent product, resorufin, was monitored by the present
optical system. To confirm the performance of the optical system, fluorescence signals
obtained by a photomultiplier through a long pass filter from the reservoirs containing
resorufin solution (Fig. 2a) at different concentrations were detected. The results areshown in Figs. 3. For monitoring the enzyme reaction of HRP with Amplex red
containing hydrogen peroxide, an HRP solution at different concentration and a mixed
solution of Amplex red and hydrogen peroxide were placed in each reservoirs of the
microchip shown in Fig. 2b. When the microchip was rotated at the speed of 1200 rpm,
two solutions in the reservoirs were started to flow into the spiral channel. Resorufin, theproduct of the enzyme reaction, was generated in the spiral channel and was detected by
the optical detector. The time-course of the fluorescence signal is shown in Fig. 4.
Flow behavior of liquids on the CD-type microchip will be also presented in the lecture.
Keywords: Compact disk type microchip, flow behavior, electrochemical detection,
optical detection

Photomultiplier

0.5 mM

ReservoirLong pass filter(561 nm)0 mM
Flow cell

Laser diode(531 nm, 5 mW))

Fig. 1 A compact disk (CD)-type microchip with
optical detection

Fig. 2 CD-type microchip (a)without and (b) with a spiralchannel

4.0 mM
3.0 mM
2.0 mM
1.0 mM
0.5 mM
0 mM

1.2

1

0.8

0.8

電圧 / VPMT signal/V

1.2

0.6

0.4

0.4

intensity/a.u.
Light signal/V
PMT

0.25
1.4

1 ppm HRP
0.1 M Amplex Red

0.2
0.20
0.15
0.15
0.1
0.10
0.05
0.05

0.2

0

0 0 0010
10(72 s)

0
0

0

100

100

200

300

200 300
時間 / us

400

400

500

HRP

2 mM

3 mM

600

500 600

Time/ms
Fig. 3 PMT signal from resorufin
solution of different concentration placed
in reservoirs (Fig. 2a).

0.20

PMT signal/V

Optical fiber

1 mM

4 mM

Turn table

Amplex Red

0.15
0.10
0.05
0

9.5

20
20

500 ms1010.5

Time/ms
30
40
30
40

Time/sTime/s1150
50(122 s)Fig. 4 PMT signal from resorufin
produced from enzyme reaction of Amplex
red with HRP containing H2O2 (Fig. 2b).

８５

Acknowledgements
This research was supported in part by the Japan Society for the Promotion of Science(JSPS) KAKENHI Grant No. 25288065.
Reference:
1. A. Hemmi, T. Usui, A. Moto, T. Tobita, N. Soh, K. Nakano, H. Zhen, K. Uchiyama, T.
Imato, H. Nakajima, J. Sep. Sci., 34, 2913-2919 (2011).
2. S. Guo, K. Nakano, H. Nakajima, K. Uchiyama, A. Hemmi, Y. Yamasaki, S. Morooka,
R. Ishimatsu, T. Imato, Pure App. Chem., 84, 2027-2043 (2012).
3. S. Guo, R. Ishimatsu, K. Nakano, T. Imato, Talanta, 133, 100-106 (2015).
4. P. Rattanarat, P. Teengam, W. Siangproh, R. Ishimatsu, K. Nakano, O. Chailapakul, T.
Imato, Electroanal., 27, 703–712 (2015).
Introduction to the author:
Professor Dr. Toshihiko Imato
B S (1976), M.C. (1978), Kyushu University, Japan
Dr. of Engineering (1988), Kyushu University under supervision by Prof. N. Ishibashi.
Research Associate (1978), Kyushu University.
Associate Professor (1988), Kyushu University.
Professor (1993), Kyushu University.
Chairperson of Japanese Association for Flow Injection Analysis (2013 - ).

８６

KL.19LC-MS/MS analysis of sphingolipid metabolites in mice during acuteexercise stressYong-Moon Lee
College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea
Abstract: Extracellular sphingosine 1-phosphate (S1P) binds to a family of five G-protein
coupled-receptors, S1P1-5. In most vessels, S1P binds to S1P2 which mainly expressed in
vascular smooth muscle cells to cause vasoconstriction. In arteries, the S1P exposure
triggers Rho kinase and RhoA, the activator of Rho kinase translocation to the plasma
membrane in response to stimulation with S1P. Thus the effects of S1P on blood vessel
may have pathophysical relevance to S1P concentrations. Therefore, S1P signaling
pathways are potential therapeutic targets for the treatment of hypertension, stroke andvasospasm. The present investigation focused on the concentration changes of S1P and
its metabolites in blood, heart, lung and muscles during swimming exercise stress in mice.
In high stress conditions, S1P concentration was obviously decreased (27% reduction inblood, 38% reduction in heart) while greatly increased in lung (194% increased) andmuscles (375% increased). For acute exercise, S1P may be urgently required for rapid
contraction of smooth muscle cells in lung and muscles. The ceramides synthesis is
impaired in vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats
where may contribute to increased VSMC proliferation in hypertension. Indeed, in
exercise condition, ceramide levels were slightly decreased 12% in blood and 20% inheart, respectively. Interestingly, ceramide levels in lung and in muscle were apparently
increased. It is not still well understood why sphingolipid metabolites is increasing or
decreasing by outer stress or pharmacological agents depending on the organs. Specifically,
the increase of ceramide in lung may be contributed to the concomitant activation ofneutral sphingomyelinase (nSMase) and acidic sphingomyelinase (aSMase). In addition,high S1P increase (almost 4- fold) in muscle is contributed by the activation of
sphingosine kinase, PKC-dependent enzyme synthesizing S1P from sphingosine byphosphorylation. Taken together, it should be mentioned that S1P and ceramides
concentrations in organs were found to be greatly changed in acute exercise conditions
and provides additional evidence for the significance of S1P and ceramides in the
cardiovascular system.
８７

Keywords: LC-MS/MS, sphingolipid, sphingosine 1-phosphate, ceramide, blood
Reference:
1. Sphingolipid analysis by high performance liquid chromatography-tandem mass
spectrometry (HPLC-MS/MS). Bielawski J, Pierce JS, Snider J, Rembiesa B, Szulc ZM,
Bielawska A. Adv Exp Med Biol. 2010;688:46-59
2. Vascular endothelium as a contributor of plasma sphingosine 1-phosphate.
Venkataraman K, Lee YM, Michaud J, Thangada S, Ai Y, Bonkovsky HL, Parikh NS,
Habrukowich C, Hla T. Circ Res. 2008 Mar 28;102(6):669-76
Introduction to the author:
Professor Dr. Yong-Moon Lee
College of Pharmacy, Chungbuk National University, Korea
Tel: +82-43-2612825; Fax: +82-43-2682732.
E-mail: ymleefn@chungbuk.ac.kr
Biography of the authors.
Professor Lee received his Ph.D from University of Tokyo on the chemical derivatizaiton
for the determination of bioactive carboxylic acids after dansyl cadaverine derivatizaiton.
After he got the professor position at Chungbuk National University in 1990, he haveworked on the development for the screening tool of chemopreventive natural products tocancer treatment. Since he developed analytical method with chemical derivatizaiton from1996, he newly focused on the lipid biological mechanism in sphingolipid metabolic
pathway. He developed various chemical derivatizaiton for sphingolipid analysis,
including sphingolipid metabolic enzymes assay for finding specific inhbitors from natural
resources. He currently worked with the analysis of harmful chemicals in drugs in
supported by KFDA Research fund. He has published over 85 SCI papers and 5 reviews.

８８KL.20Strategy toward the ultrafast separation
Yi Chen*Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Abstract: How to accelerate a separation is an interesting study topic becoming more andmore crucial because we are facing more and more complex analytical objects andsamples. Speeding up of a separation is also an urgent challenge in omic studies, and it isin theory easy but in practice tough. Herein we will take capillary electrophoresis (CE) as
an example to discuss the issue. A strategic thinking to speed up the separation lies inchromatographic theory. According to Van Deemter equation, there are at least four ways
available, including shortening the separation length, increasing a driving force such as
electric field strength, use of better separation materials, and reduction of injection length.
Use of a short separation length is easily realized but stopped by a heavy loss of resolution.
Although the loss could partially or even largely be recovered by increase of the applied
electric field strength, it will be limited by the self-heating effect. Exploitation and use of ahighly effective separation medium like photonic crystals (PCs) were then considered andtired, and high resolution and ultra-fast separation have been achieved [1]. This is a newgate toward the acceleration of separations but has to be guaranteed by significant
reduction of the sample zone length. This needs a new sample injection method. Although
there were optical and hydrodynamic gating injection methods published in literature [2,3],
they are hardly copied to a common laboratory. A simpler approach termed diffusionbased injection (D2I) was hence exploited in this laboratory. It could simply be realized bytouching a capillary tip on a sample surface for a while, able to inject a sample plug of lessthan 20 μm into the capillary tip. D2I was shown to be suitable for the injection of not only
liquid but also solid and in-between-stated samples. To the best of our knowledge, this is
the first method allowing a direction injection of solid samples. It is by use of D2I that CE
can now separate some small molecules such as amino acids within seconds. For detailed
discussions please join me on the conference.
Keywords: Fast separation strategy, capillary electrophoresis, solid sample injection
Reference:
Liao T, Guo Z, Li J, Liu M, Chen Y. Lab Chip 2013, 13, 706-713.
Monnig C. A., Jorgenson J W. Anal. Chem. 1991, 63, 802-807.
８９

Lemmo A V, Jorgenson J W. Anal. Chem. 1993, 65, 1576-1581.
Introduction to the author:
Professor Dr. Yi Chen
CAS Key Lab of Analytical Chemistry for Living Biosystems, Institute of Chemistry,
Chinese Academy of Sciences, Beijing 100190, China
Tel: +86-10-62618240; Fax: +86-10-62559373.
E-mail: chenyi@iccas.ac.cn
Biography: Dr. Yi Chen currently holds a position of professor both at Institute ofChemistry, Chinese Academy of Science (CAS), and University of CAS. He has beenworking on capillary electrophoresis since 1984, on surface plasmon resonance sensing
and imaging since 1997, and on bio-mass spectrometry since 2006, with more than 230
publications, three books and 30 patents. Dr. Chen received twice the First-class Award of
Sciences and Technology from the China Association for Instrumental Analysis, Young
Scientist Award from CAS, Young Scholar Award from the Hong Kong Qiushi Scienceand Technology Foundation, and Young Chemist Award from the Chinese Chemical
Society. He is also a Titular Member of Analytical Chemistry Division, IUPAC; theAssociate Editors of Anal. Methods, Chinese Anal. Chem., Chinese J. Chromatogr., andAnal. Instrumentations; and members in the editing or advisory boards of J. Chromatogr.
A, J. Chromatogr. B, Sci. China B, Chinese J. Chem., Progress Chem. etc.(15 journals in
total). He has been elected the vice presidents of Chinese Society of Chromatography,
Beijing Society of Chromatography, Beijing Chemical Society, and Branch of Analytical
Instruments at China Instrument and Control Society.

９０KL.21

Microfluidic Chips towards Cell Signaling Analysis
Pen Chen, Yao Wang, Bi-Feng Liu*
College of Life Science and Technology, Huazhong University of Science and Technology,
Wuhan 430074, China
Abstract: Cell signaling has been recognized as a highly dynamic biological process. The
involved highly dynamic signals are regulated both by complicated intracellular processes,
such as cell signal transduction network, gene regulatory network, metabolic regulatory
network, protein interaction network and by the fluctuations in extracellular environment,
including gradients of cytokines and secreted proteins from neighboring cells, biochemical
and mechanical interactions with the extracellular matrix and direct cell-cell contacts.
Analysis of these dynamics, therefore, required combination of two critical technologies:
high resolution imaging and high precise perturbation. As is well-known, there have been
tremendous progress in high resolution imaging, however high precise perturbation has
been still an immense challenge. Currently, investigation of cell signaling usually involves
dynamic imaging of cells upon localized mechanical, electrical, or chemical perturbations.
Chemical perturbation is a relatively gentle approach that can mimic the surrounding
environmental signals to cells in vivo, which is preferred for uncovering such dynamic.
Conventionally, chemical perturbations are implemented via perfusion systems, whichhave been useful in providing information about cell signaling. However, their limitations
are impeding further development, especially the drawbacks in lack of precision for spatial
and temporal control of chemical stimuli. In this presentation, we report a precision guided
microfluidic chemical perfusion strategy for cell dynamic signaling with high spatial andtemporal resolution. It provided a versatile platform for studying cell signaling inindividual cells or among contacting cells, capable of addressing individual cells among acell cluster without interfering with adjacent contacting cells. The high temporal resolution
further allowed pulsing cells with varying durations, which can be a potentially useful tool
for research in chemical biology, cell biology and pharmacology.
Keywords: microfluidics, cell signaling, chemical perfusion
Reference:
1. El-Ali J, Sorger PK, Jensen KF (2006) Nature 442:403–411
９１

2. Chen P, Feng X, Sun J, Wang Y, Du W, Liu BF (2010) Lab Chip 10:1472–1475
Introduction to the author:
Professor Dr. Bifeng Liu
College of Life Science and Technology, Huazhong University of Science and Technology,
Wuhan, China
Tel: +86-27-87792203; Fax: +86-27-87792170.
E-mail: bfliu@mail.hust.edu.cn
Biography of the authors.
Dr. Bifeng Liu received his Ph.D. degree of analytical chemistry in 1999 from Wuhan
University (China) where he continued his postdoctoral research in the College of Life
Science. From 2001, he moved to Japan as a JSPS postdoctoral fellow in Prof. S. Terabe‟sgroup. He joined Huazhong University of Science and Technology (China) as a professor
in 2003. He is now serving as Deputy Dean of the College of Life Science and Technology.
His research focuses on systems biology-oriented analytical science in the areas of
microfluidics (or lab-on-a-chip) for bioanalysis, mass spectrometry coupled microseparations for functional proteomics & molecular imaging. He is the author and co-authorof over 100 original research papers and invited reviews published in international
journals.

９２

KL.22Polycyclic Aromatic Hydrocarbons, Nitropolycyclic Aromatic Hydrocarbons and Inorganic Ions in Snow Layers at Murodo, Tateyama,Japan
Kazuichi HAYAKAWA1,2*, Genki SUZUKI1, Fumiya MAKINO1, Hiroshi MORISAKI1,
Ning TANG1, Akira TORIBA1 and Kazuma AOKI3
1

Institute of Medical, Pharmacutical and Health Sciences, Kanazawa University, Japan
Institute of Nature and Environmental Technology, Kanazawa University, Japan
3
Graduate School of Science, Toyama University, Japan
2

Introduction:
Acidic rain/snow and Asian dust storm in Japan are caused by long range transports ofsulfur oxides and yellow sand, respectively. Recently, PM2.5 has become another big
environmental issue in East Asia, because PM2.5. Polycyclic aromatic hydrocarbons (PAHs)
and nitropolycyclic aromatic hydrocarbons (NPAHs) are formed through imperfectcombustion of organic matters such as fossil fuels and wood, and they are major
components of PM2.5. We found the atmospheric concentrations of PAHs and NPAHs at
the Noto peninsula, Ishikawa, Japan, increased in the period from mid. October to mid.
April every year, and identified that PAHs and NPAHs were mainly emitted from coal
combustion processes, including coal heating, in north-east China, These results suggestthat snow in Japan might be an useful environmental sample for identifying long-range
transport of not only yellow sand but also PM2.5, because snow falls on the Japan Sea
coast area of Japan by the north-west wind in winter. The author reported thedetermination of PAHs and inorganic ions in snow collected at Murodo, Tateyama, Japanat CJK2014. In this report, we collected cake samples from different snow layers at
Murodo in April 2015, and determined not only PAHs and inorganic ions but also NPAHs
for the detail discussion of sources and behaviors of PM2.5.
Experimental:
Snow cakes were collected from four different layers (L-I, L-II, L-III and L-IV, in April,2013 and M-I, M-II and M-III, in April 2015) at Murodo (2,450 m above sea level),
Tateyama, Japan. Each snow cake, more than 1 kg each, was melted at room temperature,and the solution was filtered with a GC filter (pore size 0.5 μm). After drying, the filter
９３was weighed to calculate the amount of particulates collected. Nine inorganic ions (Cl -,NO3-, SO42-, Na+, NH3+, K+, Ca2+ and Mg2+) were determined by ion chromatography. Ten
PAHs having four to six rings (fluoranthene (FR), pyrene (Pyr), benz[a]anthracene (BaA),
chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BbF), benzo[a]pyrene(BaP), dibenz[ah]anthracene (DBA), benzo[ghi]perylene (BghiPe) and indeno[1,3,3cd]pyrene (IDP)) were determined by HPLC with fluorescence detection according to ourprevious report.1) Three NPAHs (1- and 2-nitropyrenes (1- and 2-NPs) and 2nitrofluoranthene (2-NFR)) were determined by HPLC with chemiluminescence detection
according to our previous report.2)
Results and Discussion:
From the depth of snow, dates of four layers, L-I through VIII and M-I through III,
were estimated. In L-series, L-I showed the lowest concentration of particulates and L-III
showed the highest concentration of particulates. L-IV showed the highest concentrationof nssCa2+. L-IV showed the highest concentrations of PAHs and nssSO42-. On the other
hand, in M-series, M-I showed the highest concentration of nssSO42-, PAHs and NPAHs.
Back trajectory analysis suggested that air masses corresponding to L-IV mainly passed
through central-east China and north-east China, respectively and that air mass
corresponding M-I mainly passed through north-east China. These results suggest that
PM2.5 long-range transported from north-east China contained high concentration of PAHs,
NPAHs and nssSO42-. The smaller NPAH/PAH concentration ratios, such as [1-NP]/[Pyr],suggested that the major contributor was coal-burning systems but not automobiles.
Monitoring of particulate weight only is not sufficient for identifying the origin of PM2.5.
Simultaneous determination of PAHs and inorganic ions are very important to differentiate
the origins of PM2.5, yellow sand and combustion of fossil fuels.
Acknowledgements This work was supported by the Environment Research andTechnology Development Fund (Project No. B-0905) of the Ministry of the Environment,
Japan, and Grants in Aid for Scientific Research (Project No. 21256001) from the
Ministry of Education, Culture, Sports, Science and Technology, Japan.
Keywords: polycyclic aromatic hydrocarbons, nitro polycyclic aromatic hydrocarbons,inorganic ions, snow, PM2.5, yellow sand, Tateyama
References:
Yang, X.-Y., Okada, Y., Tang, N., Matsunaga, S., Tamura, K., Kameda, T., Toriba, A.,
Hayakawa, K., Long-range transportation of polycyclic aromatic hydrocarbons from
China to Japan. Atmos. Environ., 41, 2710-2718 (2007).
Tang, N., Sato, K., Tokuda, T., Yang, X., Tatematsu, M., Hama, H., Suematsu, C., Kameda,
９４

T., Toriba, A., Shima, M., Hayakawa, K., Factors affecting atmospheric 1, 2-nitropyrenes
and 2-nitorfluoranthene in winter at Noto peninsula, a remote background site, Japan,
Chemosphere, 107, 324-330 (2014)
Dr. Kazuichi Hayakawa
Professor of Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmacutical and
Health Sciences/Director of Institute of Nature and Environmental Technology, Kanazawa
University
Kakuma-machi, Kanazawa, 920-1192 Japan
Tel: 81-76-234-4413; Fax: 81-76-234-4456; E-mail: hayakawa@p.kanazawa-u.ac.jp

９５KL.23
Determination of Important Volatile Compounds from Aqueous Matrix
Sunyoung Bae*
Department of Chemistry, Seoul Women’s University
Abstract
Volatile fractions of samples are generally associated with low molecular weightcompounds that vaporize at room temperature from various matrices. For various reasons,volatile compounds become important to be measured and characterized. Either major orminor in composition, they play important roles in many applications such asenvironmental, clinical, forensic, personal-care, pharmaceutical, and food-product samples.
The trace compounds could be more significant than major compositions. However, due toits characteristic including the loss of compounds during sample preparation, sensitive
analytical techniques are required.
Sample preparation is so difficult in trace analysis that becomes the rate-determining stepof the experiment. Through preparation of the analytes, it enables minor compounds to beconcentrated and detected over the limit of detection of the analytical instruments. Several
sample preparation techniques would be discussed about advantages and disadvantages
with experimental data in this presentation. They include solid phase microextraction fiber
located in a needle (SPME), in-tube SPME, in needle capillary adsorption trap (INCAT),solid phase dynamic extraction (SPDE), single drop microextraction (SDME) and asorbent-packed needle trap device (NTD).
Especially, sol-gel microextraction phases for sample preparation were investigated.
Polydimethylsiloxane (PDMS) has been generally used for non-polar analytes whileothers used for polar compounds. In our lab, the different sol-gel microextraction phases
were produced including PDMS/DVB (Divinylbenzene), PDMS/PEG (Polyethylene
glycol), PDMS/PAA (Polyacetic acid), and PDMS/β-CD (Cyclodextrine). Needle was aplatform to be coated with these phases. It is easy to handling and inexpensive to fabricate.
It also shows that the selectivity and extraction efficiency of both polar and nonpolar
compounds depending on coating materials in needle.
Data obtained from the real samples show that the minor compounds in trace amountwould distinguish one sample from others. The quantitative information of thesecompounds was input for principal component analysis as multivariate statistical analysis.
This approach provides the fundamental information on characterization of volatilecompounds for samples and their classification for recognition.
９６

Keyword: Volatile compounds, sol-gel microextraction, classification, preconcentration

References:
1. Sohn, H-H., Bae, S., and Lee, D.S. 2012. New needle packed with
polydimethylsiloxane having a micro-bore tunnel for headspace in-needle microextractionof aroma components of citrus oils, Anal. Chim. Acta, 751, 86-93
2. Jeon, H-L., Son, H-H., Bae, S., and Lee, D. S. 2015. Use of Polyacrylic acid and
Polydimethylsiloxane Mixture for In-Needle Microextraction of Volatile Aroma
Compounds in Essential Oils, Bull. Korean Chem. Soc. 36, 11 (in press)

Introduction to the author:
Dr. Sunyoung Bae
Department of Chemistry, Seoul Women‟s University, Seoul, Korea
Tel: +82-2-970-5652; Fax: +82-2-970-5972
Email: sbae@swu.ac.kr
Biography of the author:
Dr. Sunyoung Bae has obtained Ph.D. from the University of Massachusetts-Lowell in
2002. She had been working at the interdisciplinary research center at the University ofNorth Carolina-Charlotte. In 2008, she was appointed as an assistant professor in the
Department of Chemistry at North Carolina A&T State University. Since 2010, she has
been working at Seoul Women‟s University. Her research approach is to characterize
physicochemical properties and interactions in micro-scale using analytical instrumentsthat determines macro-phenomena. Dr. Bae‟s research topics include separation andsampling methods for GC, GC-MS, biomass conversion and its characterization. Throughsome projects that she has conducted, her main role is to integrate fundamental analyticalchemistry into a problem-solving solution to a problem. Dr. Bae had been served as aguest editor for Journal of Hazardous Materials (Elsevier), Environmental Monitoring andAssessment (Springer), and Journal of Materials in Civil Engineering (ASCE). Currently,
she has been served as an associate editor for Korean Society of Soil and Groundwater
Environment and on the editorial board for the Korean Society of Analytical Sciences.

９７OL.01
Electrochemical study on electron transfer across DNA base pairs
Jifeng Liu*
School of Food Engineering and Biotechnology, Tianjin University of Science andTechnology, Tianjin 300457, China.
Abstract:
The molecular -stack of base pairs within double-strand DNA (dsDNA) has beenshown to mediate electron transport (ET) reactions, which has received a substantial
attention because of its biological significance in DNA damage and DNA repair
mechanisms as well as the DNA electronic devices. The ET process across the base pairstacking as well as the bases terminated monolayer were investigated.
1. How far can hydroxyl radicals travel?
Onto gold surface adsorbed DNA monolayer composed of double stranded part of 20
base pairs at the gold suface and extra single stranded (AT)n part at the distal of DNA
monolayer. TiO2@-Fe2O3 composite nanoparticles were attached onto the distal of single
strand DNA parts. Under UV illumination, hydroxyl radicals generated at the TiO2 shellwould diffuse to the double strand parts of DNA monolayer and the single strand parts
acted as ruler to measure the diffusing distance of the radicals. Thus using photocatalytic
reactive nanoparticles and DNA composite modified gold electrodes, exploiting thesensitivity of DNA-mediated electron transfer to base pair stacking, we proposed anelectrochemical technique to read the effective diffusing distance of hydroxyl radicals.
Scheme1.

(left)

Preparationof

TiO2@-Fe2O3 core-shell particle
attached dsDNA modified Au
electrode(Fe2O3@TiO2/(AT)n/dsDNA/Au)(step 1, 2 and 3) and oxidation of
DNA base pairs by photogenerated •OH radicals upon UV illumination (step 4 and 5).
(right) The single strand part of DNA ((AT)n, n = 5, 12, 24) acted as a spacer between basepair in the dsDNA film and the Fe2O3@TiO2 particle when placed a magnet above themodified surface. Top, n  12; bottom, n  12.
2. An electrochemical study based on thymine-Hg-thymine DNA base pairs mediated９８

electron transfer process
Onto Au surface adsorbed DNA monolayer composed of double stranded of 25 basepairs, and at the gold surface and distal part were matched base pairs, while at the middleof DNA monolayer were (TT)n mismatched pairs. The ET properties of the DNA
monolayer was studied using Laviron‟s theory and it was found that when n  6, thekinetics of CT followed the order: matched DNA base pairs  DNA duplexes with (TT)n 
DNA duplexes with (T-Hg(II)-T)n and the CT kinetics increased with increasing n.
However, an interesting phenomenon was found when n  12, that is (T-Hg(II)-T)nconformation was formed between different DNA strands and the this inter-DNA (THg(II)-T)n structure caused DNA monolayer deformed and CT not occur along the DNAbase pairs.

Scheme 2. CT process of dsDNA with
(T-Hg(II)-T) sequench. (a) matched dsDNA, (b) dsDNA with (T-Hg(II)-T)1, (c) dsDNA
with (T-Hg(II)-T)3, (d) dsDNA with (T-Hg(II)-T)6, (d) dsDNA with (T-Hg(II)-T)12.
3. Study on electron transfer across nucleobases terminated surface assembly
ET process with different kinetics occurred across the nucleobases terminated multilayer
assembly and the base-dependent ET properties were studied in this work. To prepare such
nucleotide monophosphate (NTMP) (including guanosine, adenosine, cytidine, uridine-5´monophosphate, and abbreviations were GMP, AMP, CMP, UMP respectively) assembly,
3-mercaptopropionic acid, Mg2+, NTMP were adsorbed onto Au substrates stepwise toform nucleobases terminated surface. Cyclic voltammetry, chronoamperometry andelectrochemical impedance spectroscopy showed that the rate of ET kinetics decreased in
the order: GMP/Mg2+/MPA/Au > AMP/Mg2+/MPA/Au > UMP/Mg2+/MPA/Au >CMP/Mg2+/MPA/Au. Nucleobases have different geometrical conformations and electricalstructural properties, which determined the ET kinetics across nucleobases terminatedmultilayer assembly. And these differences in ET behavior were ascribed to different
HOMO energies of the Mg-Nucleotide moieties (decreased in the order: GMP-Mg >AMP-Mg > UMP-Mg > CMP-Mg). This study may be valuable to the studies of DNA
electronic structure, which had been utilized for DNA sequencing via nanopore techniques.

９９

eMg

0

Mg-NTMP

MPA

LUMO

-1
-2
-3
-4

The energy of orbital (eV)The energy of orbital(ev)

Au

base

Scheme 3. Energy diagram for the electrontransfer from the gold electrode to nucleobases
across the NTMP/Mg2+/MPA/Au multilayer
assembly. The calculation was carried out basedon B3LYP/6-31G* using Gaussian program.

-4.4
-4.8

LUMO

-5.2
-5.6

HOMO

-6.0
-6.4
Mg-GMPMg-AMPMg-UMPMg-CMP-5-6
Keywords:
DNA base pairs, electron transfer, electrochemistry-7

Au

MPA Mg-GMP----

Reference:[1] Q. Guo, Q. Yue, J. Zhao, L. Wang, H. Wang, X. Wei, J. Liu, J. Jia. Chem. Commun. 47(2011) 11906-8.
[2] E. Xu, Y. Lv, J. Liu, X. Gu, S. Zhang. RSC Adv. 5 (2015) 49819-23.
Introduction to the author:
Professor Dr. Jifeng Liu
School of Food Engineering and Biotechnology, Tianjin University of Science andTechnology, Tianjin, China.
Tel: +86-22-60602795; Fax: +86-22-60602795.
E-mail: jfliu@tust.edu.cn
Biography of the author.
Dr. Jifeng Liu, received his PhD in Analytical Chemistry in 2004 from ChangchunInstitute of Applied Chemistry (CIAC), Chinese Academy of Sciences (CAS). After
postdoctoral research at É cole Polytechnique Fédérale de Lausanne (EPFL), he came to
Liaocheng University in October 2007 and was a Taishan Schoolar professor. In January
2015, he joined the Key Laboratory of Food Nutrition and Safety, (MOE), Tianjin
University of Science and Technology as a professor. His research interests areelectrochemistry of bio-nanomaterial interface, electrocatalysis, and application ofelectrochemistry in real analysis.

１００

OL.02
Application of novel nanomaterials in the phosphopeptide enrichment
Liping Li, Linnan Xu, Wen Ma, Huwei Liu, Yu Bai*
College of Chemistry and Molecular Engineering, Peking University, Beijing, China
Abstract: As one of the most common post-translational modifications, protein
phosphorylation is of great importance in regulating various biological processes.
Therefore, effective analytical methods for phosphoprotein are in urgent demand forfurther phosphoproteomic research. Mass spectrometry (MS) is considered as a powerfultool in the detection and identification of proteins. However, for phosphoproteomic
analysis, effective enrichment approaches prior to MS analysis are often required due toserious signal suppression caused by non-phosphorylated species. Among theseapproaches, metal oxide affinity chromatography (MOAC) has become more and more
popular in recent years [1-3]. Different surface properties of nanomaterials often lead todifferent enrichment performance in sensitivity, specificity or phosphoproteome coverage.
Therefore, the exploration of novel nanomaterials for comprehensive phosphoproteomic
analysis with enhanced sensitivity is still attracting considerable interest. In this work,
binary metal oxides and other novel nanomaterials have been synthesized andcharacterized. All these novel nanomaterials provide attractive properties in thephosphopeotide enrichment and the target proteomic research [4-7].
Keywords: nanomaterials, MS, affinity probe, enrichment
Reference:
1. D. F. Stern, Exp. Mol. Pathol., 2001, 70, 327.
2. A. Leitner, TrAC, Trends Anal. Chem., 2010, 29, 177.
3. L. P. Li, L. N. Xu, Z. Li, Y. Bai*, H. W. Liu, Anal. Bioanal. Chem., 2014, 406, 35.
4. L. P. Li, T. Zheng, L. N. Xu, Z. Li, L. D. Sun, Z. X. Nie, Y. Bai, H. W. Liu, Chem.
Commun. 2013, 49, 1762.
5. L. N. Xu, L. P. Li, L. Jin, Y. Bai, H. W. Liu, Chem. Commun., 2014, 50, 10963.
6. L. P. Li, J. Z. Liu, L. N. Xu, Z. Li, Y. Bai, Y. L. Xiao, H. W. Liu, Chem. Commun., 2014,
50, 11582.
7. L. P. Li, S. Chen, L. N. Xu, Y. Bai*, Z. X. Nie, H. W. Liu, L. M. Qi, J. Mater. Chem. B,
2014, 2, 1121.

Introduction to the author:
１０１

Associate Professor Dr. Yu Bai
College of Chemistry and Molecular Engineering, Peking University, Beijing, China
Tel: +86-10-62758198; Fax: +86-10-62751708.
E-mail: yu.bai@pku.edu.cn
Biography of the authors.
Dr. Yu Bai is an Associate Professor of Analytical Chemistry at Peking University. She
obtained her Ph. D in chemistry from the Changchun Institute of Applied Chemistry,
Chinese Academy of Sciences, in 2004. She did her post-doc in University of Toronto
during 2005-2008. She got the Chinese Natural Science Foundation--Outstanding YouthFoundation in 2014. Her major research interests focus on i) Application of nanomaterials
in the separation and analysis of biological samples; ii) Novel ambient ionization method
and its application; iii) Disease related metabolomics and proteomics.

１０２

OL.03
Antioxidant Activity of Volatile Compounds Isolated from Broccoli(Brassica oleracea L.) sprouts by GC and GC-MS
Hae Won Jang
Food Analysis Center, Korea Food Research Institute, Korea
Abstract: Broccoli (Brassica oleracea L.) sprouts have received much attention recently
because they contain various bioactive constituents, which are not present in broccoli.
However, there are no reports on the biological activity of the volatile compounds frombroccoli sprouts. In the present study, volatile extracts of broccoli sprouts were obtainedby a steam distillation under reduced pressure, followed by a liquid–liquid continuous
extraction and then examined for their antioxidant activities using aldehyde/carboxylic
acid and lipid/malonaldehyde (MA) assays. All samples exhibited dose-dependent
antioxidant activity. The antioxidant activity ranged from 74.5 ± 0.5% (less volatilesample) to 99.5 ± 0.5% (volatile sample) at the level of 500 μg/mL. Antioxidant activity ofsamples obtained from broccoli sprouts was comparable to that of known antioxidants, αtocopherol and BHT. Subsequently, volatile extracts were analyzed by gas
chromatography and gas chromatography–mass spectrometry to identify principle
antioxidants. Among the 43 compounds positively identified by gas chromatography/mass
spectrometry, 4-methylpentyl -isothiocyanate (2.48 μg/g) was found in the greatest
concentration,
followedby
4-methylthiobutylnitrile(10.63μg/g),
5methylthiopentylnitrile (31.64 μg/g), 3-methylthiopropylisothiocyanate (3.00 μg/g), and 4methylthiobutylisothiocyanate (14.55 μg/g). These isothiocyanates are known to possess
antioxidant properties. Possible phenolic antioxidants found are 4-(1-methylpropyl)phenol (0.012 μg/g), 4-methylphenol (0.159 μg/g) and 2-methoxy-4-vinylphenol (0.009μg/g). The present study demonstrates that broccoli sprouts are a good source of natural
antioxidants.

１０３

Figure 1. GC Chromatograms of cholesterol in infant formulas using GC/FID.
Keywords: broccoli sprouts, isothiocyanates, natural antioxidants, volatile compounds
Reference: Jang, H.W., Moon, J.K., Shibamoto, T., (2015), Journal of Agriculture andEnvironmental chemistry, 63, 1169-1174.
Introduction to the author:Dr. Hae Won Jang
Food Analysis Center, Korea Food Research Institute, 463-746, Korea
Tel: +82-31-780-9377; Fax: +82-31-780-9280.
E-mail: hwjkfri@kfri.re.kr
Biography of the authors.
Dr. Hae Won Jang has obtained Ph.D. in agriculture and environmental chemistry atUniversity of California, Davis in 2013. After the graduation, she has been working inFood Analysis Center at Korea Food Research Institute. Her research and interest areas
include biological and chemical studies on extracts from natural plants and maillard
reaction systems.

Determination of arsenic species in food by LC-ICP-MS
Cheong-Tae Kim
Food Safety Research Institute, NONGSHIM CO., LTD, Seoul, Korea

１０４Abstract: Arsenic (As) is considered as one of the most important toxic elements in theenvironment because of its potential risk to human health and to ecosystems. Apart fromdrinking water, rice consumption may be the most important pathway of human As uptake.
After the US-Government announced that the rice cultivated in California has high levels
of inorganic As, many consumer groups are concerning about human health problems will
be caused by staple food. Especially, it is especially alarming in Asian countries due tohuge consumption of rice. The chronic exposure to As can lead to cancerous healthhazards and other health problems. So, we conducted the development of extraction
method for arsenic speciation and the determination of arsenic compounds in food stuff
including rice grain purchased from markets in Korea by using LC-ICP-MS.
Around 1.0 g samples were weighed into the vessels and extracted with 10 mL of 0.28 M
nitric acid at 85 ℃ for 70 min. Samples were cooled to room temperature andcentrifuged at 4500 rpm for 10 min. The supernatant was filtered and kept at 4℃until
analysis. Using a solution of 1% HNO3 for extracting As from food stuff was suitable.
Arsenic species were quantified by IC-ICP-MS with Dionex IonPacTM AS7 RFICTM
column (250 mm x 2 mm). The gradient mobile phases were 10 mM ammonium carbonate
adjusted to pH 8.8 and 200 mM ammonium nitrate and ammonium phosphate adjusted topH 9.0. The flow rate was 0.3 mL/min. Injection volume and column temperature were 25
uL and 15 ℃, respectively.
Arsenic exists in forms of various chemical species. Inorganic As species (arsenite (As3+)
and arsenate (As5+)) have been classified as carcinogenic and the methylated form,
monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) have been identified ascancer promoters. It is believed that arsenobetaine (AB) and arsenocholine (AC) are nontoxic. The method of As speciation by IC-ICP-MS was developed that allowed thesimultaneous separation of six As species. Four species of As were mainly detected infood stuff as As3+, As5+, MMA and DMA. Arsenic species in samples were extracted withmicrowave-assisted 1 % nitric acid. The recoveries of fortified rice (50 ug/kg) were
91~112 % The LODs of As species method were 0.1~ 0.5 ug/kg.
Keywords: Arsenic, Speciation, LC/ICP/MS, Rice, Food stuff
Reference(1) Ronkart SN, Laurent V, Carbonnelle P, Mabon N, Copin A, Barthelemy JP.,
Chemosphere 2007,66, 738-745.
(2) Nakamura Y, Narukawa T, Yoshinage J., J. Agric Food Chem. 2008,56, 2536-2540.
Introduction to the author:
１０５

Dr. Cheong-Tae Kim
Food Safety Research Institute, NONGSHIM CO., LTD, Seoul, Korea.
Tel: +82-2-820-8131; Fax: +82-2-810-9047.
E-mail: kctmass@hanmail.net
Biography of the authors:
Dr. Cheong-Tae Kim completed his doctorate in food technology with “Reduction ofacrylamide in fried foods by addition of amino acids and vacuum frying.” at Seoul
National University in 2004. He has been worked at NONGSHIM CO. LTD since 1990.
Dr. Kim has served a head of Food Safety Research Institute in his company from 2009 topresent.
Dr. Kim has responsible for the food safety of raw materials and final products in his
company. He has researched on the development and application of chemical analysis
methods by using Mass spectrometer such as orbitrap-MS and LC/ICP/MS etc. And He
has focused on studies of how to control microorganisms in products and plant facilities.
Dr. Kim has been managing the all kinds of food safety compounds such as pesticides,
antibiotics, GMO, allergens, food-born pathogen, irradiation foods and radioactive
substances under the food safety policy of his company. His research and activity arefocused on studies of chromatographic separation and identification and how to control
the issues caused by biological materials.

１０６

OL.04
Water Quality Monitoring of Drainage River in Kuantan, Malaysia byusing Ion-exclusion/Ion-exchange Chromatography
Daisuke Kozaki1*, Norhasmira Idayu binti Harun1, Nobutake Nakatani2, Masanobu
Mori3, , Kazuhiko Tanaka3
1
2

Faculty of Industrial Sciences and Technology, University Malaysia Pahang, Malaysia
Department of Environmental and Symbiotic Sciences, College of Agriculture, Food andEnvironmental Science, Rakuno Gakuen University, Japan
3
Graduate school of Science and Technology, Gunma University, Japan
Abstract:
The water quality of drainage river (system), namely Galing River in Kuantan, Malaysia
were examined effectively by using ion-exclusion/cation-exchange chromatography withwater quality indices and land usage data. In Kuantan, there are 228 sewerage treatment
plants, many of which were built in the early 1970‟s and individual septic tanks (ISTs)
serve 51%, the centralized sewer system serves 47%, and individual primitive systems
serve the remaining 2% of sewage/household wastewater in Kuantan. However, ISTs are
not highly efficient in removing nutrients and organic compounds (COD) with removal
capacities of 5–18% and 50–78%, respectively. In this study, we have focused on
evaluating and grasping the effect of sewage/household wastewater discharged fromhousing areas in the Kuantan district on the river water quality. Based on this study, thefollowing beneficial information were obtained effectively by using ion-exclusion/cationexchange chromatography: (1)Galing River are linked with the urbanization level of the river basin area; (2) differencesin the biological reactions in the different pollution level rivers are understood; (3) Galing
River is among the most polluted rivers not only in Kuantan, but also in the Malay
Peninsula, owing to poor water treatment of the sewage/household wastewater discharged
from the river basin area.
Keywords: Ion-exclusion/cation-exchange chromatography, Urban drainage river,Malaysia

１０７

Introduction to the author:
Dr. Daisuke Kozaki
Faculty of Industrial Sciences and Technology, University Malaysia Pahang, Lebuhraya
Tun Razak, 26300 Gambang Kuantan, Pahang Darul Makmur, Malaysia
Tel: +60-9-549-2413; Fax: +60-9-549-2766; E-mail: daisuke@ump.edu.my
Biography of the authors:
Current research interests are the development of the water quality monitoring methods byusing ion chromatography (IC) / high performance liquid chromatography (HPLC) andthat application to the environmental monitoring in developed and developing countries.

１０８

OL.05
Analytical Applications of Higher-order Calibration Methods Based on
Alternating Multilinear Decomposition Algorithms
Hai-Long WU* and Ru-Qin Yu
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry andChemical Engineering, Hunan University,Changsha 410082, China. E-mail:hlwu@hnu.edu.cn
Abstract:
Higher-order calibration methods based on multilinear models in chemometrics are
gaining more and more attention in the field of analytical science. Based on the“mathematical separation”, higher-order calibration methods enable one to achieve direct
quantitative analysis of analytes of interest in complex systems even in the presence of
unknown, uncalibrated interferents, which is also known as the “second-order advantage”.
Additionally, higher-order calibration methods can provide some other advantages, such as
higher selectivity and sensitivity. In this work, higher-order calibration methods based onalternating multilinear decomposition algorithms are firstly introduced and its generalprocedure, by using a simulated EEMs data array, is illustrated. Secondly, the analyticalapplications of these higher-order calibration methods in such sciences as environment,
food, biology, medicine are provided. Finally, some case studies to further illustrate thepractical analytical applications of second-order and third-order calibration methods were
provided.
Keywords: Higher-order calibration; Mathematical separation; Alternating multilinear
decomposition; Multilinear model; Second-order advantage; Direct quantitative analysis;
Complex system; Chemometrics
Acknowledgements: The authors gratefully acknowledge the National Natural Science
Foundation of China (Grant No. 21175041, 21575039) and the National Basic Research
Program (No. 2012CB910602) for financial supports.
References:[1] A.C. Olivieri, Recent advances in analytical calibration with multi-way data. Anal.
Methods. 4 (2012) 1876-1886.
１０９

[2] A. Smilde, R. Bro, P. Geladi, Multi-way analysis with applications in the chemical
sciences. Chichester: Wiley; 2005.
[3] P. Gemperline, Practical guide to chemometrics. 2nd ed. Boca Raton: CRC press; 2006.
[4] S.D. Brown, R. Tauler, B. Walczak, Comprehensive chemometrics: Chemical andbiochemical data analysis. Amsterdam: Elsevier; 2009.
[5] H.L. Wu, Y. Li, R.Q. Yu, Recent developments of chemical multiway calibration
methodologies with second-order or higher-order advantages. J. Chemom. , 2014, 28: 476489.
[6] H.L. Wu, Y. Li, C. Kang, R.Q. Yu. Chapter 3. Multiway calibration based onalternating multilinear decomposition. In: Alejandro C. Olivieri, Graciela M. Escandar,
Héctor C. Goicoechea, Arsenio Muñoz de la Peña, Eds., Fundamentals and Analytical
Applications of Multi-way Calibration. Elsevier, 2015, pp83-166 .
[7] H.L. Wu, C. Kang,Y. Li, R.Q. Yu. Chapter 4. Practical analytical applications ofmultiway calibration methods based on alternating multilinear decomposition. In:
Alejandro C. Olivieri, Graciela M. Escandar, Héctor C. Goicoechea, Arsenio Muñoz de laPeña, Eds., Fundamentals and Analytical Applications of Multi-way Calibration. Elsevier,
2015, pp167-246.

１１０Professor Hai-Long WU
Prof. Dr. Hai-Long WU
State Key Laboratory of Chemo/Biosensing & Chemometrics
College of Chemistry and Chemical Engineering
Hunan University
Changsha 410082, China========================================================
Tel/Fax: +86(731) 88821818
E-mail: hlwu@hnu.edu.cn; hlwu529@gmail.com

Dr. Hai-Long Wu, born in 1961, is a Professor of Analytical Chemistry of the College ofChemistry and Chemical Engineering at Hunan University since 1999 and the Former
Director of the State Key Laboratory of Chemo/Biosensing & Chemometrics (Hunan
University). He received his BS in 1982, M Sc in 1985 and Dr Sc in 1992 in Analytical
Chemistry from Hunan University. he worked at Hunan University during 1985-1993,
then went to Japan and received his Dr Eng in Applied Chemistry from Chiba University(Japan) in 1998. Dr. Wu has published over 200 papers in chemometrics and chemicalsensors in international journals. He was a winner of The Second Prize of the National
Natural Sciences Award of China in 2003 and a winner of the Liang Shuquan‟s Award on
Fundamental Research in Analytical Chemistry from The Chinese Chemical Society.

１１１

OL.06
Preparation and Application of Microextraction Probe for Enrichmentof Organics from Aqueous Matrices
Hai-Fang Li, Guilan Huang, Jin-Ming LIN*
Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods andInstrumentation, Tsinghua University, Beijing 100084, China.
Corresponding author email:jmlin@mail.tsinghua.edu.cn
Abstract Fiber-based microextraction has dominant advantages for sample pretreatment byintegrating sampling, extraction, and GC or LC sample introduction into one step. In
recent years, fiber microextraction is generally represented by solid-phase microextraction(SPME) and liquid-phase microextraction (LPME) [1, 2]. The former works by extractionof analytes from liquid solution onto the fiber‟s solid surface coating, while the latter isrealized by immobilizing organic solvent inside the porous membrane of hollow fiber
serving as the acceptor phase. In our work, different fiber-based microextraction methods
were developed for rapid extraction of trace organic components from aqueous matrices.
The poly(ethylene glycol) diacrylate (PEG-DA) coated glass fiber and multilayergraphene bonded titanium fiber were fabricated, which presented excellent stability andextraction efficiency [3, 4]. The multilayer-graphene modified fiber could stand up for
more than 200 SPME cycles. In addition to SPME methods, the polypropylene hollow
fiber was directly used for LPME. Strong hydrophobic organic solvent was injected into
the hollow fiber and held by the porous membrane. Dynamic hollow fiber LPME
demonstrated good extraction ability with toluene as extraction phase for extraction oforganic UV filters in cosmetic products [5]. To increase the extraction speed and efficiency,
short hollow fiber immobilized with organic solvent was vortex microextraction ofphthalate esters from aqueous matrices [6]. With the extraction solvent lost gradually fromthe hollow fiber during stirring, the efficient extraction was maintained by the absorptionof analytes in the porous membrane. The super high enrichment factor over 1500 was
obtained for phthalate esters and reduced the limit of detection as low as ng L-1 level withchromatographic analysis.
This work was supported by National Natural Science Foundation of China (21275088,
21227006).
Keywords: Fiber probe, solid-phase microextraction, liquid-phase microextraction
１１２

References
Pena-Pereira, F.; Lavilla, I.; Bendicho, C., TrAC-Trends Anal. Chem. 2010, 29, 617-628.
Zhang, B.-T.; Zheng, X.; Li, H.-F.; Lin, J.-M., Anal. Chim. Acta 2013, 784, 1-17.
Fei, T.; Li H.-F.; Ding, M., Ito, M., Lin, J.-M. J. Sep. Sci. 2011, 34, 1599-1606.
Zhang, B.-T.; Li, H.-F.; Zheng, X.; Teng, Y.; Liu, Y.; Lin, J.-M., J. Chromatogr. A 2014,
1370, 9-16.
Yang, H.; Li, H.-F.; Ito, M.; Lin, J.-M. Sci. China Chem. 2011, 54, 1627-1634.
Huang, G.; Li, H.-F.; Zhang, B.-T.; Ma, Y.; Lin, J.-M. Talanta 2012, 100, 64-70.
Biography of the author
Dr. Haifang Li
Department of Chemistry, Tsinghua University, Beijing, China
Tel: +86-10-62797463
E-mail: lihaifang@mail.tsinghua.edu.cn
Dr. Hai-Fang Li got her PhD degree in the Research Center for Eco-Environmental
Sciences, Chinese Academy of Sciences in 2005, then as a postdoctoral follow at the
Department of Chemistry in Tsinghua University during 2005-2007. She is currently asenior engineer special for mass spectrometers. She is the author and co-author of 72
research papers published in international journals. She interests in sample pretreatment
and chromatography /mass spectrometry analysis.

１１３

OL.07
Multifunctional fluorescent optical sensors for bioanalysis
Yanqing Tian
Department of Materials Science and Engineering, South University of Science andTechnology of China
Abstract: Optical sensors are important tools for cellular imaging and analysis, for probing
cellular metabolism, for understanding pathways and biological and physiologicalprocesses, and for diagnosing diseases and cancers. We have developed several series offluorescent optical sensors (pH, oxygen (O2), potassium ion (K+), and glucose sensors)
suitable for intracellular and/or extracellular imaging and analysis.
We integrated individual sensors as dual sensors using polymeric synthesis techniques for
simultaneous multi-parameter measurements. Of particular value for application in thecomplex biological environment, some of the sensors emit two or even three colors in
response to the same excitation wavelength, enabling ratiometric measurements andproviding superior measurement accuracy. For example, we have prepared a dual pH/O2
sensor with three emission colors. The tri-color sensor composed of a blue emitter as aninternal build-in reference probe, a green emitter as the pH probe, and a red emitter as theoxygen sensor. The build-in reference probe is unresponsive to pH or O2. It wasdemonstrated that this polymer-based tri-color sensor is capable for measuring the changesof pH and oxygen concentrations in photosynthesis cyanobacteria possessing highautofluorescence. We also integrated glucose and oxygen sensor as ratiometric dual
glucose/oxygen sensors for analysis the glucose and oxygen consumption for bacterial
cells and mammalian cells in real time. This dual glucose/oxygen sensor enables
simultaneous measurement of glucose and oxygen, providing important information forcontinuous monitoring of glucose, diagnosing diabetes and hypoxia (low oxygen) relateddiseases and cancer, as well as the understating of biological processes of the metabolism.
Keywords: pH sensors, oxygen sensors, glucose sensors, dual sensors
Reference:
1. Liqiang Zhang, Fengyu Su, Sean Buizer, Hongguang Lu, Weimin Gao, Yanqing Tian,
Deirdre Meldrum, “A dual sensor for real-time monitoring of glucose and oxygen”
Biomaterials, 2013, 34(38), 9779-9788.
１１４

2. Hongguang Lu, Yuguang Jin, Yanqing Tian, Weiwen Zhang, Mark R. Holl, Deirdre R.
Meldrum, “New ratiometric optical oxygen and pH dual sensors with three emission
colors for measuring photosynthetic activity in Cyanobacteria” Journal of Materials
Chemistry 2011, 21, 19293-19301.
Introduction to the author:
Professor Dr. Yanqing Tian
Department of Materials Science and Engineering, South University of Science andTechnology of China No 1088, Xueyuan Rd., Xili, Nanshan District, Shenzhen,
Guangdong, China 518055
Tel: +86-755-88018997; Fax: +86-755-88018904.
E-mail: tianyq@sustc.edu.cn
Biography:
Yanqing Tian received his B.S. and M.S. degrees from the Department of Organic
Chemistry, Jilin University (China) in 1989 and 1992. In 1995, he received his Ph.D.
degree in Polymer Chemistry and Physics from Jilin University. He had been working at
Jilin University as an associate professor, Sagami Chemical Research Center as a STA
Fellow, Tokyo Metropolitan University as a JSPS Postdoc Fellow, University ofWashington as a postdoc and then an acting instructor, and the Biodesign Institute at
Arizona State University as an assistant research professor and then an associate researchprofessor. He is now working at South University of Science and Technology of China asa professor. His research interests include synthesis and applications of functional
materials like optical sensors and block copolymers for bio-sensing, bio-imaging and drugdelivery; nanostructured block copolymer for improving non-linear optical materials‟ coefficiency and for enabling the application of hydrophobic two-photon absorbing materials
for sensing and imaging in biological conditions; and nanostructured photo-isomerizableliquid crystals. In the analysis research field, he has led his team for developing various
types of fluorescent biosensors including pH sensors, oxygen sensors, zinc ion sensors,
glucose sensors, and potassium ion sensors as well as their integrated multifunctional
materials for cell metabolism understanding and disease diagnosis. He has co-authored110 refereed papers and has 3 issued patents.

１１５

OL.08Development of a Hybrid Pyrolyzer for GAS CHROMATOGYAPHY
Naoki Oguri*, Toshio Tsuchiya, Kaoru Enokido, Tamami Takeda and Akira Onishi
Japan Analytical Industry Co., Ltd.
Abstract:
We have been developing chromatographic instruments, for instance, Curie point
pyrolyzers Curie point purge and trap samplers, recycling preparative HPLC and thoserelated attachments since 1965.
On this occasion, the 50th anniversary, we havepioneered the hybrid pyrolyzer in the world.
The pyrolyzer can be pyrolyzed polymer sample by furnace or Curie point heating byselection of the heating method.
Also, it can be used for evaluated gas analysis (EGA) for the polymers with temperaturerising as like as termogravimetry heating. And more, evaluated gas can be trapped into
the mini-PAT (packed Tenax GR) according with interest. After trapping the mini-PAT
can be desorbed and re-injected into the GC by Curie point heating in order tocomposition analysis of the peak.
Application for the polymer characterizations will be presented.
Keywords: Curie point pyrolyzer, furnace pyrolyzer, hybrid pyrolyzer
Reference:
1. Design and Application. A Curie point pyrolyzer, Naoki Oguri, Poongzag Kim,
International Laboratory, 19, 58 (1989)
2. GC-MS 用ポータブル型キューリーポイント試料導入装置の開発
大栗直毅、武田珠余、遠藤 誠、土屋俊雄、分析化学、Vol. 62, No. 7, 699(2013)
Introduction to the author:
Dr. Naoki Oguri
208 Musashi, Mizuho, Tokyo 190-1213 Japan
Tel: +81-42-557-2331; Fax: +81-42-557-1892
E-mail: n-oguri@jai.co.jp

１１６

OL.09
Facile preparation of a novel benyl imidazolium ionic liquids hybridmonolithic column for residue analysis of nitrogen-heterocyclic pesticides
by CECYan Huang, Yankai Chen, Yanxia Tang, Xiaoping Wu*
MOE Key Laboratory of Analysis and Detection Technology for Food Safety; College ofChemistry, Fuzhou University, Fuzhou, China
Abstract: Recently, hybrid organic-silica monolithic column have received much attention
in capillary electrochromatography (CEC) and LC, due to their unique advantages of easy
fabrication, pH stability, and less shrinkage [1]. C18-incorporated organic-silica
monolithic column was introducd as a prevalent separation media by Malik and coworkers[2] for RP CEC separation of the majority of non-polar and weak-polar organic
compounds. However, in the case of polar and charged compounds, the selectivity of C18
stationary phase is not that ideal due to the lack of more retention mechanisms.
Nitrogen-heterocyclic pesticides are a type of broad-spectrum and polar insecticides orfungicides which are widely used to control plant diseases. Since discovering its chronic
mammalian toxicity such as infertility, maximum pesticide residue limits (MRLs) havebeen reduced. Efficient separation and determination of nitrogen-heterocyclic pesticides
residue in the plants, water and soil is significant to keep foodsafety and human health.
Herein, a simple thermal-treatment “one-pot” approach for the preparation of hybrid
capillary monoliths with mix-mode interaction mechanisms was developed via a sol–gel
process, by using tetramethoxysilane and vinyltrimethoxysilane as precursors and 1vinylbenzyl-3-methylimidazolium tetrafluoroborate (VBIM) as functionalized monomer
respectively, in the presence of porogenic agents (PEG and urea). The effects of the ratio
and content of reaction mixture, composition of porogenic solvent and initiator, on themorphology and column performances of the hybrid monoliths were investigated. The
resulting monolith showed uniform monolithic network, high permeability andthermal/mechanical stability. It exhibits a typical reversed-phase chromatographic
behavior with the controllable EOF over a wide pH range, owing to the incorporation ofILs, which could be used for the separation of various neutral, basic and polar analytes by
CEC. A column performance varying from 84,000 to 106,600 N/m was achieved foralkylbenzenes.
Based on a mix-mode retention mechanism of hydrophobic interaction and weak
１１７

interaction, especially electrostatic interaction between organic and inorganic parts, theproposed VBIM- hybrid monolithic column has been applied for the residue analysis ofnitrogen-heterocyclic pesticides in vegetables by CEC. Under the optimum conditions,rapid separation of six nitrogen-heterocyclic pesticides was achieved within 10 min, withan interday RSD less than 6.5%. The linear range was 0.5 - 100 mg/L and the LODs
ranged from 0.05 to 0.2 mg/L. This method was successfully applied to the determinationof nitrogen-heterocyclic pesticides in vegetables with satisfying recoveries. The result incomparative experiment agrees well with that of HPLC. This research provides a novel
separation medium and feasible method for the high-efficient analysis of nitrogenheterocyclic compounds with different physical properties.
Keywords: capillary electrochromatography, ionic liquids hybrid monolithic column,
nitrogen-heterocyclic pesticides
Reference:
1. Junjie Ou, Hui Lin, Zhenbin Zhang,Guang Huang, Jing Dong, Hanfa Zou.
Electrophoresis 2013, 34, 126–140.
2. Hayes,J.D., Malik,A., Anal.Chem.2000,72,4090–4099.
Acknowledgements:
This work was supported by National Natural Science Foundation (Grant No. 21375019),
and National Instrumentation Program (2011YQ150072) of China.
*Introduction to the author:
Professor Dr. Xiaoping Wu
College of Chemistry, Fuzhou University, Fujian, China
Tel & Fax: +86-591-22866131.
E-mail: wapple@fzu.edu.cn
Biography of the authors.
Dr. Xiaoping Wu is a Professor of Analytical Chemistry at Fuzhou University (China). She
joined Fuzhou University in 1997, and received Ph.D. degree in Physical Chemistry from
Fuzhou University in 2002. Aferwards she worked as a postdoc at Fudan University,
Unimicro Tech. Inc. and Vrije Universiteit Brussel, respectively. Her research interests
cover capillary electrochromatography, ionic liquids-based monoliths, biomimetic
electrochemical sensors, and their application in foodsafety and environmental analysis.
She has published more than 60 papers in these area. She is a member of the International
Society of Electrochemistry and the Chinese Chemical Society.

１１８

OL.10New strategies for the functionalization of polymer monolith andapplications to separation and preconcentration of organic compounds
Haijiao Zheng, Gang Chen, Qiong Jia*
College of Chemistry, Jilin University, Changchun, 130012, China
Abstract: Polymer monolith column is a new type of miniaturized sample pretreatment
strategy based on in-tube solid phase microextraction. As an extraction medium, organic
polymer monolith column has several advantages of good biocompatibility, highenrichment efficiency, simple preparation process, wide pH range, and little sample
consumption, etc. It combines concentration, extraction, separation, purification, moreover,
it is rapid, sensitive, simple operation, and easy to realize automation. However, it has
some disadvantages, such as swelling in organic solvent and decreasing mechanicalstability, which may affect its life.
Macrocyclic compounds have a variety of applications, such as molecular recognition,
supramolecular self-assembly, and bionic materials. In recent years, they have been
applied in the enrichment of material and has developed very rapidly. Cyclodextrin andcucurbituril are representatives of macrocyclic compounds, both of which have cavities
and can easily form host guest compounds. Hydrogen bond, hydrophobic, and π-πinteractions may exist between them and other compounds. In addition, they both have avariety of functional groups on the surface, which makes them easily modified. In thepresent study, cyclodextrin and cucurbituril were employed for the modification of organic
polymer monolithic column and used as enrichment materials. The modified polymer
monolithic columns were coupled with chromatography and applied to the determinationof organic compounds in food, environment, and biological source.
Keywords: Polymer monolith, functionalization, preconcentration
Reference:
1. Zheng, H. J.; Liu, Q. W.; Jia, Q. J. Chromatogr. A 2014, 1343, 47.
2. Zheng, H. J.; Li, Z.; Ma, J. T.; Zhou, Y. F.; Jia, Q. RSC. Adv. 2015, 5, 5850.
3. Liu, D.; Song, N.; Cheng, Y, C.; Chen, D, X.; Jia, Q.; Yang, Y, W. RSC. Adv. 2014, 4,49153.
4. Qi, R. F.; Zhou, X.; Li, X. Q.; Ma, J. T.; Lu, C. M.; Zhang, X. G.; Jia, Q. Analyst 2014,
139, 6168.
１１９

5. Zhang, J. L.; Chen, G.; Tian, M. M.; Li, R. G.; Quan, X, J.; Jia, Q. Talanta 2013, 115,801.
6. Tong, S. S.; Zhou, X.; Zhou, C. H.; Li, Y. Y.; Li, W. J.; Zhou, W. H.; Jia, Q. Analyst
2013, 138, 1549.
7. Li, W. J.; Zhou, X.; Tong, S. S.; Jia, Q. Talanta 2013, 105, 386.
8. Tong, S. S.; Liu, Q. W.; Li, Y. C.; Zhou, W. H.; Jia, Q.; Duan, T. C. J. Chromatogr. A
2012, 1253, 22.
Introduction to the author:
Professor Dr. Qiong JIA
College of Chemistry, Jilin University, Changchun, 130012, China
E-mail: jiaqiong@jlu.edu.cn
Prof. Jia, born in 1974, obtained her PhD degree in Changchun Institute of Applied
Chemistry, Chinese Academy of Sciences in 2003. Her research interests focus on
separation and preconcentration methods. She has published about 100 scientific papers inthis area.

１２０

OL.11
Electrodialytic Matrix Isolation and Preconcentration for Ionic Solutes
Shin-Ichi Ohira*, Takayuki Yamasaki, Kei Toda
Department of Chemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555,
Japan
Abstract: Ionic solutes determination is widely applied for an evaluation of environmental
and human health conditions, and industrial products. The determinations can be achieved
with fully automated instruments such as ion chromatography (IC), high performanceliquid chromatography (HPLC), induction coupled plasma (mass) spectrometry and so on.
However most of samples cannot be directly introduced into the instruments because of
hard matrices which may interfere the analysis or break the instruments. Most of cases,
sample pretreatment, which removes matrices to purify the sample, was applied to every
sample batchwisely. Previously, we have developed electrodialytic matrix isolation system
for ionic solutes analysis [1–3]. The developed ion transfer device for matrix isolation wasconsisted with five solution flow channels separated with ion permeable membranes. Ionic
solutes are selectively and quantitatively transferred from a sample into acceptor solutions,typically purified water. Non-ionic compounds, particulates, and proteins are nottransferred. Based on the quantitative ion transfer, the enrichment of ionic solutes insample was also achieved with a higher sample flow rate and smaller acceptor solutions.
This in-line preconcentration can be applied to determine trace analyte without highly
sensitive analytical instruments.
Tap water disinfection is generally achieved by addition of sodium hypochlorite.
However, it is also reported that more than 600 kinds of byproducts such as trihalometanes,
and chloroacetates are generated. These by-products cause cancers or liver damage. In
Japan, the quality standards for tap water was set as 0.02, 0.04, and 0.2 mg L-1 formonochloroacetic acid (MCA), dichloroacetic acid (DCA) and trichloroacetic acid (TCA),
respectively. Chloroacetates in tap water are measured with gas chromatograph–massspectrometry (GC–MS), GC–electron captured detector (ECD), and high performanceliquid chromatography–MS (HPLC–MS) for the evaluate tap water safety. Chloroacetes
can also be measured with an ion chromatography (IC) with conductivity detection, and a
HPLC with UV absorbance detection. However, the limits of detections of the IC or
HPLC-UV for the compounds is not enough to detect the quality control levels.
In the present study, we have tried to determine chloroacetic acids in drinking waterwith conventional IC with UV absorbance detection. The sample, drinking water, was１２１

introduced into the electrodialytic ion transfer device without any pretreatment. Obtained
acceptor solution was directly introduced ion chromatography system. Because
chloroacetic acids are weak acids and relatively larger sample flow rates are required forin-line enrichment, larger applied voltage (~50 V) was required for the quantitativetransfer and enrichment. The obtained limits of detection (LOD) with x10 in-line
enrichment was 0.004, 0.003, and 0.02 mg L-1 for MCA, DCA, and TCA, respectively.
These values were enough to detect Japanese quality standard for tap water. The
recoveries were studied with real tap waters (n = 5). The obtained recoveries were
89~110%.
Keywords: Matrix isolation, electrodialysis, preconcentration, haloacetic acids
Reference:
1. S. Ohira, K. Kuhara, M. Kudo, Y. Kodama, P.K. Dasgupta, K. Toda, Anal. Chem., 84,5421–5426 (2012).
2. S. Ohira, K. Kuhara, A. Shigetomi, T. Yamasaki, Y. Kodama, P.K. Dasgupta, K. Toda, J.
Chromatogr. A, 1372, 18–24 (2014).
3. S. Ohira, Y. Hiroyama, K. Nakamura, P.K. Dasgupta, K. Toda, Talanta, 132, 228–233(2015).
Introduction to the author:
Dr. Shin-Ichi Ohira
Department of Chemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860–8555,
Japan. Tel: +81–96–342–3384; Fax: +81–96–342–3384.
E-mail: ohira@sci.kumamoto-u.ac.jp
Biography of the authors.
Dr. Ohira received his Bachelor (2000), Master (2002), and Doctor (2005) of Science
degrees from Kumamoto University. He served as Research Fellow for Young Scientists of
the Japan Society for the Promotion of Science (JSPS) at Kumamoto University (2005 –2006), Postdoctoral Fellow for Research Abroad of JSPS at Texas Tech University (2006)
and The University of Texas at Arlington (UTA) (2007−2008), and Postdoctoral Research
Fellow at UTA (2008−2009). He was promoted to his present position in 2009. Hereceived Awards for Younger Researchers from the Japan Association for Flow Injection
Analysis (2012), Ion Chromatography Discussion Group of the Japan Society for
Analytical Chemistry (JSAC) (2012), and the JSAC (2013). His current research interests
are the developments of novel analytical devices for flow-based electrodialytic ion transfer
between solution phases, trace gas detection with metal organic frameworks, and detection
on HPLC.
１２２

OL.12
Antioxidant Activity of Volatile Compounds Isolated from Broccoli(Brassica oleracea L.) sprouts by GC and GC-MS
Hae Won Jang
Food Analysis Center, Korea Food Research Institute, Korea
Abstract: Broccoli (Brassica oleracea L.) sprouts have received much attention recently
because they contain various bioactive constituents, which are not present in broccoli.
However, there are no reports on the biological activity of the volatile compounds frombroccoli sprouts. In the present study, volatile extracts of broccoli sprouts were obtainedby a steam distillation under reduced pressure, followed by a liquid–liquid continuous
extraction and then examined for their antioxidant activities using aldehyde/carboxylic
acid and lipid/malonaldehyde (MA) assays. All samples exhibited dose-dependent
antioxidant activity. The antioxidant activity ranged from 74.5 ± 0.5% (less volatilesample) to 99.5 ± 0.5% (volatile sample) at the level of 500 μg/mL. Antioxidant activity ofsamples obtained from broccoli sprouts was comparable to that of known antioxidants, αtocopherol and BHT. Subsequently, volatile extracts were analyzed by gas
chromatography and gas chromatography–mass spectrometry to identify principle
antioxidants. Among the 43 compounds positively identified by gas chromatography/mass
spectrometry, 4-methylpentyl -isothiocyanate (2.48 μg/g) was found in the greatest
concentration,
followedby
4-methylthiobutylnitrile(10.63μg/g),
5methylthiopentylnitrile (31.64 μg/g), 3-methylthiopropylisothiocyanate (3.00 μg/g), and 4methylthiobutylisothiocyanate (14.55 μg/g). These isothiocyanates are known to possess
antioxidant properties. Possible phenolic antioxidants found are 4-(1-methylpropyl)phenol (0.012 μg/g), 4-methylphenol (0.159 μg/g) and 2-methoxy-4-vinylphenol (0.009μg/g). The present study demonstrates that broccoli sprouts are a good source of natural
antioxidants.

１２３

Figure 1. GC Chromatograms of cholesterol in infant formulas using GC/FID.
Keywords: broccoli sprouts, isothiocyanates, natural antioxidants, volatile compounds
Reference: Jang, H.W., Moon, J.K., Shibamoto, T., (2015), Journal of Agriculture andEnvironmental chemistry, 63, 1169-1174.
Introduction to the author:Dr. Hae Won Jang
Food Analysis Center, Korea Food Research Institute, 463-746, Korea
Tel: +82-31-780-9377; Fax: +82-31-780-9280.
E-mail: hwjkfri@kfri.re.kr
Biography of the authors.
Dr. Hae Won Jang has obtained Ph.D. in agriculture and environmental chemistry atUniversity of California, Davis in 2013. After the graduation, she has been working inFood Analysis Center at Korea Food Research Institute. Her research and interest areas
include biological and chemical studies on extracts from natural plants and maillard
reaction systems.

１２４

OL.13
Electrothermal vaporization or atomization on tungsten coil for
analytical atomic spectrometry
Xue Jiang,a Chenghui Li,a Xiaoming Jiang,b Zhou Long,b Xiandeng Hou*a,ba

Key Laboratory of Green Chemistry & Technology, Ministry of Education, College ofChemistry, Sichuan University, Chengdu, Sichuan 610064, China;b
Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan 610064, China*

Corresponding author. Tel. & Fax: (+) 86-028-8540518; E-mail: houxd@scu.edu.cn.

Tungsten coil device has been used in analytical atomic spectrometry for approximately
40 years for sample vaporization or atomization (1). These vaporizers/atomizers can beeasily electrically heated up to over 3000 K at very high heating rates, with a simple
power supply, and the whole device can be very compact. Here we report our recent work
using tungsten coil as an electrithermal vaporizer for sample vaporization into atomizers
such as new plasmas and flames, and as an atomizer or excitation source, for atomic
spectrometry (2,3).
References
1 X.D. Hou, B. T. Jones, Spectrochim. Acta, 2002, 57 (4), 659-688.
2 P. Wu, Y.C. Zhang, R. Liu, .i Lv, X.D. Hou, Talanta, 2009, 77 (5), 1778-1782.
3 X.M. Jiang, Y. Chen, C.B. Zheng, X.D. Hou, Anal. Chem., 2014, 86 (11), 5220-5224

１２５

OL.14
Functionalized Gold-Nanomaterials Fluorescence Probe for Cyanide
Detection
Dongtao Lu; Guomei Zhang, Chuan Dong, Shaomin Shuang*
Institute of Environmental Science , College of Chemistry and Chemical Engineering,
Shanxi University, Taiyuan 030006, China.
Abstract: Cyanide is the most-concerning toxic anion due to its severe environmental
pollutants and adverse health effects. The World Health Organization (WHO) has set the
maximum permissive level of cyanide in drinking water at 1.9 μM . Fluorescent method
for the detection of cyanide exhibited many advantages over other optical methods such as
high sensitivity, easy operation, and multiplicity of measurable parameters.
Gold nanomaterials have been favorably adopted to design novel sensors byfluorescent methods due to their ultra small size, nontoxicity, and high fluorescence. We
investigate a series of gold nanomaterials fluorescent sensor for the detection of cyanide inaqueous solution. Lysozyme stabilized gold nanoclusters (Lys-AuNCs) have beensynthesized and utilized as a fluorescence probe for selective detection of cyanide (CN-).
Lys-AuNCs had an average size of 4 nm and showed a red emission at 650 nm (λex=370
nm). The fluorescence intensity was linear with the CN- concentration in the range of
5.00×10−6 M ~1.20×10−4 M with a detection limit as low as 1.9×10−7 M. Simultaneously,
the Lys-AuNCs could be visualized with the naked eye for CN- which might etch with
Lys-AuNCs resulted in an obvious color change from light yellow to colorless undervisible light (Fig.1A). N-acetyl-L-cysteine modified gold nanoparticles（NAC-AuNPs）
for CN- detection in aqueous solution have been designed, synthesized, and characterized.
NAC-AuNPs showed a red emission at 690nm (λex=370 nm) and an average size wasabout 3 nm measured by TEM. An excellent sensitivity fluorescence probe of NACAuNPs for CN- in aqueous solution was developed ranging from 1.0×10-7M~1.1×10-6M,
with a detection limit of 6.4×10-8M. In addition, the NAC-AuNPs probe possessed a good
selectivity for CN- over other common anions and was expected to be great candidate forCN- detection in food, water and biological applications (Fig.1B). In summary, Au
nanomaterial could be a novel fluorescence probe for CN- and might be a great candidate
for sensing applications in environmental analysis and fluorescence imaging.

１２６

A)

B)

ClO 4

EDTA

C6H5O73-

-

2-

SCN

NO2

-

Ac

C2O4

-

I

Br

Cl

F

1.0

2-

1.2

CN-

F0/F

1.4

Fig.1 (A) The color (top) and the fluorescent (bottom) changes of Lys-AuNCs solution todifferent anions ions (concentration of all the anions ions was 3mM). (B)Selectivity of
NAc-AuNPs-based sensor for CN- (50μM) over other anions (50μM) at pH of 12.
Keywords: gold nanoparticles, cyanide detection, fluorescence probe
Reference:
1. Lu DT, Liu, LL, Li FX, Shuang SM, Li YF, Choi MMF, Dong C. Spectrochimica acta.
Part A, Molecular and biomolecular spectroscopy 2014, 121, 77-80.
2. Zhang GM, Qiao YY, Xu T, Zhang, CH, Zhang Y, Shi LH, Shuang, SM, Dong, C.
Nanoscale 2015.
Introduction to the author:
Dr. Lu Dongtao, Institute of Environmental Science, Shanxi University, Taiyuan, China
Tel: +82-351-7018525; Fax: +82-351-7011011.E-mail: ludongtao@sxu.edu.cn
Corresponding author: Prof. Shaomin Shuang, College of Chemistry and Chemical
Engineering
Tel: +82-351-7018842; Fax: +82-351-7011011.E-mail: smshuang@sxu.edu.cn
1

2

3

4

5

6

7

8

9

10

11

12

Biography of the authors.
Dongtao Lu received his bachelor‟s degree in biology and master‟s degree in hydrobiology in 2004 and 2007 from Shanxi University, respectively. He obtained his PhD
degree in environmental at Shanxi University in 2014. His research focuses on thesynthesis of metal nanomaterials and development of chemical or bio-sensors.
Guomei Zhang received her BSc degree from the Shanxi University of China(Taiyuan) in
1997, her MSc degree and PhD degree in analytical and inorganic chemistry in 2003 and2006 from Shanxi University, respectively. She joined the Chemistry Faculty of Shanxi
University in 2006, and master tutor at Shanxi University since 2008 and has been aprofessor in 2014. Her current research interests include the DNA assembly andphotoluminescence analysis of nanomaterials.
Shaomin Shuang received her BSc degree in chemistry and MSc degree in
１２７

analyticalchemistry in 1986 and 1989 from Shanxi University, respectively, and PhD
degree in1998 from South China University of Technology. She joined the chemistry
faculty of Shanxi University in 1989, and has been a professor at Shanxi University
since2002. Her current research interests include analytical chemistry and development ofbiosensors and chemosensors.
Chuan Dong is a professor of analytical chemistry at Shanxi University. He obtained his
PhD degree at Shanxi University in 2002. Dong has published over 200 papers and 100
patents and his current research interests are biosensors, chemosensors, optical fiber sensor,
fluorescence and room temperature phosphorescence, and environ-mental analysis andmonitoring.

１２８

OL.15Determination of silicate, borate and carbonate ions in natural waters
using ion-exclusion chromatography coupled with charged aerosol
detector
Nobutake Nakatani*1, Yu Otsuka1, Takuya Takahashi1, Daisuke Kozaki2, Masanobu Mori3,
Kazuhiko Tanaka31

College of Agriculture, Food and Environment Sciences, Rakuno Gakuen University,
Faculty of Industrial Sciences and Technology, University Malaysia Pahang, 3Graduate
School of Science and Technology, Gunma University
2

Abstract: Weak inorganic anions of silicate, carbonate and borate ions are the important
ionic species in biogeochemical cycles of silicon and carbon, and the water pollutants. In
the natural waters, these anions are presents in partially dissociated or un-dissociatedforms. In general, these ionic species are separately determined by the spectrophotometryor the titration method. Ion-exclusion chromatography (IEC) was developed asconventional method for separation of weak acid anions and was applied as a method forwater quality monitoring [1]. Charged aerosol detector (CAD) has become useful in theanalytical chemistry field and has been applied as a detector for liquid chromatography [2].
In this presentation, we introduces about the determination for weak inorganic anions ofsilicate, borate and carbonate ions using IEC coupled with Corona® CAD. Silicate andborate ions were separated and detected using the ultrapure water as an eluent and the
addition of acetonitrile to the elution made the sensitivity for both ions higher. Carbonate
ions could not detected due to the degradation into carbon dioxide during the drying
process to form particles in CAD. By connecting a cation-exchange column in the K+form after the ion-exclusion separation, weak inorganic anions including carbonate ions
could be converted to their potassium salts and detected by CAD within 15 min. The
present method was successfully applied to the analysis of weak inorganic anion in natural
water samples.
Keywords: charged aerosol detector, ion-exclusion chromatography, weak inorganic
anionsReference:
1. N. Nakatani, D. Kozaki, M. Mori, K. Tanaka: Analytical Sciences 28, 845 (2012).
１２９

2. K. Fukushima, K. Hashiguchi, T. Suzuki, M. Okawara, Y. Sekiguchi: Chromatography
32, 161 (2011).
Introduction to the author:
Associate Professor Dr. Nobutake Nakatani
College of Agriculture, Food and Environment Sciences, Rakuno Gakuen University, 582
Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan
Tel & Fax: +81-11-388-4714.
E-mail: nakatani@rakuno.ac.jp
Nobutake Nakatani earned his Ph.D. from Hiroshima University in 2004. He was
postdoctoral fellow in Hiroshima University under Professor Dr. Kazuhiko Tanaka andstudied ion chromatography. Since 2009, he has been an associate professor of the Collegeof Agriculture, Food and Environment Sciences at Rakuno Gakuen University.

１３０OL.16
Hairpin DNA Template-based Copper Nanoparticles for the Detection of
NAD+
Jianzhong Lu
School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, China
Ultra-small metal nanoparticles are recognized as a promising alternative to organic
fluorophores and quantum dots as they have unique physical, electrical, and opticalproperties. With regard to metal nanofabrication, DNA has proven an excellent templatefor fluorescent metal nanoparticles due to its unique nano-sized structure, excellent
programmable properties, and great affinity for several metal ions. For example, DNAtemplated gold, silver, palladium, platinum, and copper nanoparticles (CuNPs) are highly
suitable for signal amplification due to their facile synthesis, ultrafine size, low toxicity,and photophysical properties. In the specific case of CuNPs, both double-stranded DNA(ds DNA) and single-stranded poly-thymine (ss poly T) can serve as templates. Herein, we
propose a strategy utilizing hairpin-structured DNA as an efficient template for theformation of fluorescent CuNPs. A label-free analytical system based on this novel hairpin
template demonstrated excellent sensitivity for the quantitative detection of NAD+.

１３１

PT.01Development of Inkjet Printing Single-cell Technology to Electrospray
Ionization for Mass Spectrometer
Fengming Chen, Shiqi Wang, Ziyi He, Hai-Fang Li, Jin-Ming LIN*
Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods andInstrumentation, Tsinghua University, Beijing 100084, China.
Corresponding author email: jmlin@mail.tsinghua.edu.cn
Abstract Single cell analysis has become an important field of research in recent years,
high-throughput single cell measurements of cellular responses are of great importance fora variety of applications, including drug testing, toxicology, and basic cell biology [1-2].
Fluorescence microscope allows us to observe the states of cells before and after
stimulation. However, it is difficult to observe signals of a large number of cells under amicroscope. Electrospray ionization mass spectrometry (ESI-MS) is a sensitive methodwith low sample consumption, which is especially suitable for small and even ultra-small
volume analysis [3-4]. Unfortunately, many biological samples are very complicated, andthe matrices, especially those containing salts, have a significant ion suppression effect,
which enormously reduces the sensitivity of Nano-ESI-MS. Moreover, the salts in samples
can form complicated compounds through molecular association and coordination, whichmakes it quite difficult to clearly identify the target compounds in the mass spectrum. It iseven worse when using Nano-ESI-MS for the analysis of a single cell owing to its verysmall sample volume. The inkjet printing technology [5-6] can accurately andreproducibly eject picoliter volumes of droplet, with which we combined tungsten probes
for selectively and controllably encapsulating one single target cell into one picolitervolume droplet. The tungsten probes with a tip were applied for electrospray ionization ofmass spectrometry (MS) detection. We report a highly accurate sample injection system torealize the single-cell mass spectrometry based on an inkjet technology.
This work was supported by National Natural Science Foundation of China (nos.
81373373, 21227006, 91213305).

１３２

References
Gong, X.; Zhao,Y.; Cai, S.; Fu, S.; Yang, C.; Zhang, S.; Zhang, X., Anal. Chem. 2014, 86,
3809−3816.
Pan, N.; Rao, W.; Kothapalli, N. R.; Liu, R.; Burgett A. W. G.; Yang Z., Anal. Chem. 2014,
86, 9376−9380.
Liu, W.; Wang, N.; Lin, X.; Ma, Y.; Lin, J.-M., Anal. Chem. 2014, 86,7128-7134.
Zhang, J.; Wu, J.; Li, H.; Chen, Q.; J.-M., Biosensors and Bioelectronics, 2015, 68, 322–
328.
Chen, F.; Ying, R.; Weng, Y.; Lin, L.; Zeng, H.; Nakajima, H.; Uchiyama, K.; Lin, J.-M.,
Analyst, 2015, 00, 1–7.
Chen, F.; Mao, S.; Zeng, H.; Xue, S.; Yang, J.; Nakajima, H.; Lin, J.-M.; Uchiyama, K.,
Anal. Chem., 2013, 85, 7413–7418.
Biography of the author
Dr. Fengming Chen
Department of Chemistry, Tsinghua University, Beijing, China
Tel: +86-10-62792607
E-mail: chenfengmingok@mail.tsinghua.edu.cn
Dr. Fengming Chen got his PhD degree in Tokyo Metropolitan University in 2013, andthen started to work as a postdoctoral fellow at Department of Chemistry, Tsinghua
University. He is the author of four research papers published in international journals.
Now he is working on drop-on-demand inkjet printing technology for CE /mass
spectrometry analysis.

１３３

PT.02
Selective removal of cadmium from water samples by bio-functionalizedcomposite MT@C-dot@SiO2
Yi-Kun Li, Ting Yang, Ming-Li Chen*, Jian-Hua Wang
Research Center for Analytical Sciences, Northeastern University, Shenyang 110819,
China
Silica gel has been widely used as adsorbent for the removal of heavy metals due to its
good mechanical, chemical and thermal stability. However, the silanol groups on thesurface of silica gel bind with a variety of metal species and thus result in poor selectivity.
In this study, we report a bio-functionalized composite, e.g., MT@C-dot@SiO2. The
composite material provides a favorable adsorption performance toward cadmium and isused for cadmium removal from waters.
Silica gel is modified with metallothionein (MT) to improve the adsorption selectivity
through the specific interactions between mercapto group and cadmium. One-step
calcination is used to immobilize carbon dots (C-dots) onto the silica gel. The obtained
composite shows excellent chemical stability and thus it can be used repeatedly. The
MT@C-dot@SiO2 composite has been characterized by means of FT-IR, SEM and TGA.
The adsorption capability for cadmium by MT@C-dot@SiO2 is derived to be 304 μg g-1
with respect to 178 μg g-1 by pure silica gel. Although the improvement on the adsorption
capacity for cadmium is not remarkable, while most importantly, the MT@C-dot@SiO2
composite exhibits very high tolerance capacity to some of the commonly encounteredcationic and anionic species in water bodies. When 100 μg L-1 cadmium solution is treatedby 6 mg of the composite material, an adsorption efficiency of >90% is achieved in thepresence of large amount of concomitant species, e.g., 1000 mg L-1 of K+, Na+, Mg2+, Ca2+,
NO3-, HCO3- and 5 mg L-1 of Al3+, Fe3+, Cu2+, Pb2+. In general, the bio-functionalizedcomposite MT@C-dot@SiO2 is demonstrated to be a promising adsorbent for theselective removal of cadmium from water bodies.
Acknowledgement
This work is financially supported by the Natural Science Foundation of China (21375013,
21235001, 21475017).
Reference:
１３４

A. B. Bourlinos, A. Stassinopoulos, D. Anglos, Chem. Mater., 20, 4539-4541 (2008)
K. Y. Jiang, L. S. Schadler, R. W. Siegel, J. Mater. Chem., 14, 37-39 (2004)
T. Yang, L. H. Liu, J. W. Liu, J. Mater. Chem., 22, 21909-21916 (2012)
Introduction to the author:
Professor Dr. Ming-Li Chen
Research Center for Analytical Sciences, Box332, Northeastern University, Shenyang
110819, China
Tel: +86-24-83687659; Fax: +86-24-83687659.
E-mail: chenml@mail.neu.edu.cn
Professor Ming-Li Chen was born in 1979 in Henan, China. She obtained her B. Eng.
from College of Chemical Engineering, Zhengzhou University (China), and she wasawarded a PhD degree by Northeastern University, China.
Her current research interest is focused on the exploitation of flow analysis protocols andhyphenation techniques, isolation of trace metal species from complex sample matrices.

１３５

PT.03
Monitoring the dynamic mass-transferring behaviors of hemoglobin
between aqueous/ionic-liquid phases with liquid core optical waveguidesystem
Xu-Wei Chen*, Xu Yang, Wan-Ying Zeng, Jian-Hua Wang
Research Center for Analytical Sciences, Northeastern University, Shenyang 110819,
China
Ionic liquids (ILs) is a group of organic salts consisting entirely of ions (anions andcations) and exist in the form of liquid at temperature <100℃. Due to the uniqueproperties such as negligible vapor pressure, nonflammability, high chemical/thermal
stability, low toxicity and favorable conductivity, ILs have been termed as green solventsand shown great promise as an attractive alternative or replacement to conventional
volatile organic solvents in various fields. The potential of ILs in biological sample
pretreatment are also well demonstrated by their extensive applications in theextraction/purification of protein species from complex matrixes. The ILs-based liquidliquid extraction system not only achieve extraction without deteriorating the activities
and configurations of proteins, but also eliminate the risk of protein contamination whichmight pose adverse effects on the subsequent biological investigations.
As a kind of novel detecting technique, liquid core waveguide could greatly enhance thedetection
sensitivityofconventional
spectrophotometry, by extending optical path
length via the formation of total internal reflection(TIR) at the interfaces. The evanescent wave
induced by the TIR extend out from the surface ofthe higher refractive index medium and into thelower index medium, could efficiently interact
(7)

(3)

(8)

(2)

(5)

(4)

(1)

(6)

(2)

(9)

(10)

with the analyte on the liquid-liquid interface, thus
offering a powerful tool for real-time monitoring Fig. 1 Schematic diagram of ILs-based liquid core optical waveguide system.
1, Xe arc lamp; 2, optical fiber; 3, collimator; 4, support board; 5, FEP
of the local reactions at the core-cladding film(RI=1.338); 6, IL BmimPF (RI=1.408); 7, protein solution; 8, convex len;
9, spectrometer ; 10, personal computerinterfaces, and the dynamic transferring processacross the interface.
We herein develop a solid-cladding/liquid-core/liquid-cladding sandwich optical6

１３６

waveguide system to monitor the dynamic transfer of hemoglobin (Hb) from aqueous
phase to ILs phase. The optical waveguide system is fabricated by using a hydrophobicILs (1-butly-3-methylimidazolium hexafluorophosphate, BmimPF6) as the core, andprotein solution as one of the cladding layer (Fig. 1). The monitored spectra by this opticalwaveguide system indicate that the transferring of Hb molecules consists of theaggregation of Hb on water/ILs interface and the dynamic extraction of Hb into ILs phase,and part of Hb would remain at the interface even after the accomplish of the extraction
process. Investigations on the transfer rates of Hb under different pH and concentrationsuggest that the transferring of Hb from aqueous phase towards IL phase is mainly driven
by the coordination interaction between the heme group of Hb and the cationic moiety ofimidazolium ILs, and the hydrophobic interactions also contribute to the transferring
behaviors of Hb.
Reference:
Liu R, Liu JF, Yin YG, Hu XL, Jiang GB. Anal. Bioanal. Chem., 393, 871-883(2009)
Chen XW, Sakurazawa A, Sato K, Tsunoda KI, Wang JH. Applied Spectroscopy, 66(7):798-802(2012)
Introduction to the author:
Professor Dr. Xuwei Chen
Research Center for Analytical Sciences, Box332, Northeastern University, Shenyang
110819, China
Tel: +86-24-83687659; Fax: +86-24-83687659.
E-mail: chenxuwei@mail.neu.edu.cn
Dr. Xuwei Chen, Professor of Research Centre for Analytical Sciences at Northeastern
University (China), received his PhD degree in July 2007. His current research interests
focus on the isolation of trace biomacromolecules from complex biological samples.

１３７

PT.04Self-assembly sea urchin-like ZnO nanoparticles for headspace thin-film
microextraction coupled with SERS to detect sulfur dioxide in medlar
Zhuo Deng1, Ziming Zhao1, Yiru Wang1*, Xi Chen1,2**
1. Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis &Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University,
Xiamen 361005, China
2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen
361005, China
Corresponding author email: yrwang@xmu.edu.cn；xichen@xmu.edu.cn
Abstract:
Self-assembly sea urchin-like ZnO nanoparticles were used for headspace thin-film
microextraction (TFME) and coupled with surface enhanced Raman spectroscopy (SERS)
to perform a simple and easy operational determination of SO2 in medlar. After head space
sampling (HS), the highly volatile SO2 was adsorbed and concentrated by the nano ZnO,
and the content of SO2 was then measured using SERS. The method showed goodsensitivity and reproducibility. After condition optimization, it was satisfactory that sulfur
dioxide concentration and the intensity of SERS shift at 630 cm-1 showed a good linearity
in the range of 25−800 mg/kg. The detection limit of the method was found to be 15
mg/kg. The real samples analysis by the proposed HS-TFME-SERS method agreed very
well with that by the traditional distillation and titration method, showing the method was
reliable and applicable for the sulfite analysis in medlar samples. The method has shown
great potentials in the fast analysis of sulfite in various sample matrixes such as acid rain,
white sugar, and fresh or dried vegetables.
Keywords: Thin-film microextraction ZnO SERS sulfur dioxide

１３８

Fig. (a) Schematic illustration of the preparation of self-assembly sea urchin-like ZnO
nanoparticles and HS−TFME−SERS analysis of SO2 in medlar. (b) SERS spectra andworking curve of HS-TFME-SERS method for analysis of SO2 at different concentration.
The inset figure presents the simulation curve of SERS peak intensity at 630 cm-1.
Reference:
F. R.Kermani and J.Pawliszyn, Analytical Chemistry., 2012,84, 21,8990–8995.
R.Li, C.Han, Q.W.Chen, Rsc Advances.,2013, 3, 29, 11715-11722.

Zhuo Deng
Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis &Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University
Xiamen 361005, China========================================================
Tel/Fax: +86(592) 2184562
E-mail: joydeng2011@163.com
Zhuo Deng graduated from Xiamen University with a Bachelor‟s degree of chemistry in
2013. She is currently a postgraduate student at the Department of Chemistry and the
MOE Key Laboratory of Spectrochemical Analysis & Instrumentation at Xiamen
University, Xiamen. Her research interests are in the fields of pre-extraction materials
applied in surface enhanced Raman spectroscopy.
１３９PT.05
Selectively enhanced molecular emission spectroscopy of benzene,
toluene and xylene with nano-MnO2 in atmospheric ambient
temperature dielectric barrier discharge
Xue Jiang 1, Chenghui Li1, Xiandeng Hou1,2*
1. Department of Chemistry, Sichuan University, Chengdu, 610064, China
2. Analytical & Testing Center, Sichuan University, Chengdu, 610064, China
Corresponding author email: houxd@scu.edu.cn
ABSTRACT: In this work, nano-MnO2 was used to selectively enhance molecularemission spectroscopy in an atmospheric ambient temperature dielectric barrier discharge,
based on which a portable spectrometer was developed for potential field analyticalchemistry of trace benzene, toluene and xylene. Under the optimal conditions, theproposed method has some distinct advantages: (1) high selectivity and sensitivity for
detecting BTX; (2) simple, inexpensive and compact instrumentation; (3) green analyticalchemistry since no toxic reagent/solvent was used, and the exhaust is of low toxicity or at
low concentration; and (4) easy operation and hyphenation to other analytical instruments,
such as CE, GC and LC as a detector.

KEY WORDS: dielectric barrier discharge; nano-MnO2; molecular emission spectroscopy;
benzene, toluene and xylene

１４０REFERENCES
Hou, X. D.; B. T. Jones, Microchem. J. 2000, 66, 115-145.
Li, W.; Zheng, C. B.; Fan, G. Y.; Tang, L.; Xu, K. L.; Lv, Y.; Hou, X. D., Anal. Chem.
2011, 83, 5050-5055.
P. Attri; B. Arora; E. H. Choi, RSC Adv. 2013, 3, 12540-12567.
Jiang, X.; Li, C. H.; Long, Z.; Hou, X. D., Anal. Methods 2015, 7, 400-404.
Li, C. H.; Jiang, X.; Hou, X. D. Microchem J. 2015, 119, 108-113.

１４１

PT.06
Synthesis of Highly Fluorescent P-g-C3N4 Nanodots for Label-free
Detection of Cu2+ and Acetylcholinesterase Activity
Mingcong Rong1, Chunhua Ma2, Zhaochai Qiu1, Xi Chen1*
1. Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis
and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University,
Xiamen 361005, China
2. College of tea and food science, Wuyi University, Wuyishan 354300, China
Corresponding author email: xichen@xmu.edu.cn
Abstract:
Graphitic carbon nitride (g-C3N4), a well-known graphene-like carbon-based material,received a lot of attention due to its special structure and properties.1 In recent year,
nanoscale g-C3N4 materials, such as fluorescent g-C3N4 nanosheets and g-C3N4 nanodots,
have drawn great attention in sensing2 and bioimaging3 because of their excellent opticaland physicochemical features. In this report, highly fluorescent phosphorus-doped
graphitic carbon nitride nanodots (P-g-C3N4 nanodots) were synthesized by chemical
oxidation and hydrothermal etching of bulk P-g-C3N4 obtained via pyrolysis of phytic acidand melamine. The P-g-C3N4 nanodots emitted strong blue fluorescence and displayedhigh resistance to photobleaching and high ionic strength.
Copper (Cu) is a widely used industrial metal and it is an essential trace element in various
biological processes.4 The excess accumulation of Cu2+ in human body causes damage tothe central nervous system and induces neurodegenerative diseases such as Alzheimer‟sdisease, Menkes syndrome, amyotrophic lateral sclerosis and Wilson‟s diseases.5 Although
there are some attractive fluorescence sensors using carbon nanomaterials,6 it is still
essential to develop more simple, rapid and highly sensitive detection methods for
Cu2+.Acetylcholinesterase (AChE) is a type of serine esterase in the central and peripheral
nervous systems, it regulates the levels of neurotransmitter acetylcholine (ATCh) by
catalyzing the hydrolysis reaction of ATCh to choline.7 Many neurodegenerative diseases
including Alzheimer‟s disease and Parkinson‟s disease are related to the degeneration of
the cholinergic system, resulting in the amount decrease of AChE and ATCh.8Though
great efforts have been made to detect the activity of AChE, some disadvantages still exist,
such as complicated synthesis, high toxicity, expensive cost and long measurement time.
Hence, novel label-free, economic, time-saving, high sensitive and selective methods are
１４２

urgently needed.
Herein, a sensitive and facile fluorescence sensing approach for Cu2+ was developed basedon theP-g-C3N4 nanodots by photoinduced electron transfer (PET). Under optimal
conditions, a rapid detection of Cu2+ could be completed in 5 min with a detection limit of
2 nM, and a linearity ranging from 0 to 1 μM. Meanwhile, using acetylthiocholine (ATCh)as the substrate, the fluorescence of the P-g-C3N4 nanodots-Cu2+ system could besensitively turned on in the presence of acetylcholinesterase (AChE) through the reaction
between Cu2+ and thiocholine (TCh), the hydrolysis product of ATCh by AChE. A
linearity ranging from 0.01 to 3 mU/mL could be obtained with a detection limit of 0.01
mU/mL.
Keywords: P-g-C3N4 nanodots; fluorescence; Cu2+; photoinduced electron transfer (PET);
acetylcholinesterase (AChE)

Scheme.Schematic illustration of the sensitive and selective fluorescence detection of Cu2+
and AChE activity based on the P-g-C3N4nanodots

１４３

Figure.(A) Fluorescence responses of the P-g-C3N4 nanodots in the presence of different
concentrations of Cu2+ in 10 mM borate buffer (pH 7.0). (B) The relationship between
F0/F and the concentration of Cu2+. The inset figure presents the linear responses to Cu2+
ranging from 0 to 1 μM. (C) Fluorescence responses of the P-g-C3N4 nanodots in thepresence of 15 μM Cu2+ and different concentrations of AChE in 10 mM borate buffer (pH7.0). The inset figure presents the fluorescence responses of the P-g-C3N4 nanodots with
15 μM Cu2+ at low concentrations of AChE. (D) The relationship between F0/F and theconcentration of AChE. The inset figure presents the linear responses to AChE ranging
from 0.01 to 3 mU/mL.
References:(1) Wang, Y.; Wang, X.; Antonietti, M. Angew Chem Int Ed 2012, 51, 68-89.
(2) Tang, Y.; Su, Y.; Yang, N.; Zhang, L.; Lv, Y. Analytical chemistry2014, 86, 4528-4535.
(3) Zhang, X.; Wang, H.; Zhang, Q.; Xie, J.; Tian, Y.; Wang, J.; Xie, Y. Adv Mater2014, 26,4438-4443.
(4) Vedamalai, M.; Periasamy, A. P.; Wang, C. W.; Tseng, Y. T.; Ho, L. C.; Shih, C. C.;
Chang, H. T. Nanoscale2014, 6, 13119-13125.
(5) Lei, C.; Wang, Z.; Nie, Z.; Deng, H.; Hu, H.; Huang, Y.; Yao, S. Analyticalchemistry2015, 87, 1974-1980.
(6) Tian, J.; Liu, Q.; Asiri, A. M.; Al-Youbi, A. O.; Sun, X. Analytical chemistry2013, 85,5595-5599.
(7) He, T.; Qi, L.; Zhang, J.; Huang, Y.-L.; Zhang, Z.-Q. Sensors and Actuators B:
Chemical2015, 215, 24-29.
１４４

(8) Liao, S.; Han, W.; Ding, H.; Xie, D.; Tan, H.; Yang, S.; Wu, Z.; Shen, G.; Yu, R.
Analytical chemistry2013, 85, 4968-4973.

Mingcong Rong
Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis &Instrumentation, College of Chemistry and Chemical Engineering
Xiamen University
Xiamen 361005, China========================================================
Tel/Fax: +86(592) 2184562
E-mail: haiyangzhixinrmc@126.com
Mingcong Rong received her M.S. degree from the Department of Chemistry andchemical engineering at the Guangxi Normal University, Guilin. She is currently a Ph.D.
student at the Department of Chemistry and the MOE Key Laboratory of Spectrochemical
Analysis & Instrumentation at Xiamen University, Xiamen. Her research interests are in
the fields of fluorescence biosensor.

１４５

PT.07
Simultaneous detection of dihydroxybenzene isomers by HKUST-1 MOF
modified electrode
Yizhen Yang, Qingxiang Wang*
College of Chemistry and Environment, Minnan Normal University, Zhangzhou 363000, P.
R. China
Abstract: Catechol (CT), resorcinol (RS) and hydroquinone (HQ) are three typical
dihydroxybenzene isomers (DBIs) of phenolic compounds, which usually coexist inenvironmental samples as pollutants.1 Due to their high toxicity and low degradability inthe ecological environment, it is necessary to develop simple and rapid analyticaltechnology for the determination of them. The electrochemical methods attracted
increasing attentions due to their advantages of fast response, low-cost, high sensitivity
and excellent selectivity.2 However, because the three isomers of CT, RS and HQ have
similar chemical structure and close redox potentials on common electrode, thesimultaneous determination of them are usually hardly achieved. Metal-organic
frameworks (MOFs) are a type of novel three-dimensional (3D) coordination compoundswith the features of controllable synthesis, structural diversity, porosity, high specificsurface areas, metal active site richness, and high flexibility of the pore size/wall
modification.3 In this work, we fabricated a novel electrochemical sensing platform for thedetection of DBIs through covalent immobilization of the electroactive HKUST-1 MOF ona functional matrix of chitosan (CS)-electrochemically reduced graphene oxide (ERGO)
for the first time. The morphology and structure of the obtained materials and sensing
interface were characterized using scanning electron microscopy (SEM), and energydispersive X-ray spectra (EDS). The results were displayed in Figure 1

１４６

Figure 1 SEM images of CS-ERGO/GCE (A), HKUST-1/CS-ERGO/GCE with low
resolution (B) and high resolution (C), SEM (D) and EDS elemental mapping analysis of asingle HKUST-1 particle (E-G).
The electrochemical experiments showed that based on the unique porous nature andinherent redox-activity of the Cu(btc) MOF, the three DBIs of RS, CT, HQ can be well
separated from each other with a wide peak potential difference (Figure 2A). The
oxidation peak currents of HQ, CT and RS increased linearly with the concentration of
their own in the ranges of 1.0 to 200 μM (Figure 2B). The regression equation was Ipa/μA= - 0.0321 C (μM) - 5.0185 (r = 0.9951) for HQ, Ipa/μA = - 0.0256 C (μM) - 8.2104 (r =0.9900) for CT and Ipa/μA = - 0.0171 C (μM) - 2.2899 (r = 0.9918) for RS. The detection
limits were estimated to be 0.44 μM, 0.41 μM, and 0.33 μM for HQ, CT and RS,
respectively. Also the accurate determination of DBIs in the real samples could be realized
by the proposed sensor, which broadens the application of the MOF materials in theanalytical fields.
B
A

Figure 2 (A) CVs of 0.1 mM HQ, CT and RS mixture at Cu(btc)/CS-ERGO/GCE (a),Cu(btc)/CS-GO/GCE (b) and CS-ERGO/GCE (c) in 25 mM PBS (pH 7.0). (B) DPVs of
１４７

the mixtures with increasing HQ, CT and RS at Cu(btc)/CS-ERGO/GCE in 25 mM PBS(pH 7.0). Inset: the plot of Ipa versus the concentrations of RS (a), HQ (b) and CT (c).
Keywords: Dihydroxybenzene isomers, Graphene oxide, HKUST-1, Metal-organic
frameworks
Reference:
1. Perry, D. A. et al., J. Phys. Chem. C 2013, 117, 8170
2. Feng, S.; et al., J. Electroanal. Chem. 2014, 733, 1
3. Wang, X.; et al., ACS Appl. Mater. Interfaces, 2014, 6, 11573
Introduction to the author:
Professor Dr. Qingxiang Wang
College of Chemistry and Environment, Minnan Normal University, Zhangzhou 363000, P.
R. China
Tel: +86-596-2591445. Fax: +86-596-2520035.
E-mail: axiang236@126.com.
Biography of the authors.
Yizhen Yang is now a master candidate in College of Chemistry and Environment, Minnan
Normal University, majoring in electrochemical biosensors.
Qingxiang Wang received his Ph.D. in Applied Chemistry from Qingdao University of
Science and Technology in 2007. He now is a professor of the Minnna Normal University.
His research interests cover bioelectrochemistry and chemical biosensors.

１４８

PT.08In-situ growth of gold nanoparticles on mercury-binding M13 phage for
Hg2+ sensing
Xiao-Yan Wang, Ming-Li Chen, Ting Yang*, Jian-Hua Wang*
Research Center for Analytical Sciences, Northeastern University, Shenyang 110819,
China
Routine determination of mercuric ions (Hg2+) is of great importance to environmental
monitoring because of its deleterious effects on human health and environment.
Colorimetric detection and visual sensing of Hg2+ based on functionalized gold
nanoparticles (Au-NPs) have been extensively reported in the last decade, taking
advantage of the color change originates from the surface-plasmon resonance (SPR)
properties of Au-NPs. In order to enhance the selectivity towards Hg2+, thefunctionalization of Au-NPs by ligands or receptor molecules are always necessary,
making the preparation and modification process complicated and time-consuming.
Phage displayed peptide library technology is based on the expression of recombinantrandom peptides on the surface of filamentous phage. It is a new approach through highthroughput selection of peptide ligands of diversity and has been used as a powerful toolin the discovery of peptides capable of exhibiting specific affinity to various metals or
metal cations.
In the present study, a highly specific Hg2+-binding M13 phage is obtained by means of
biopanning from random heptapeptide peptide library. Positive screening against Hg(II)and negative screening against foreign metals, e.g., Fe(III), Cu(II), Zn(II) and Cd(II), areproceeded for the guarantee of selectivity. The as-selected Hg2+-binding M13 phage has
been used as bio-template for in-situ growth of AuNPs. Under optimized conditions, i.e., asystem containing 0.03 M NaOH, 631.3 μM HAuCl4 and 1010 pfu/mL phage, welldispersed AuNPs with max at 5252 nm is prepared after reaction at room temperature for
90 min. This processgreatly reduced the preparation time and simplified the preparation
procedure compared with those of previously reported approaches. In addition, the AuNPs
inherits the selectivity of Hg2+-binding phage towards Hg(II), which shows prominentcolor change in the presence of Hg2+, while the addition of other metal cations, e.g., Ag+,
Mg2+, Pb2+, Cr3+, Cd2+, Mn2+, Cu2+, Fe3+, Ni2+, and Co2+, shows no obvious change.

１４９

Pb2+ Co2+ Mn2+ Cr 3+ Hg2+ Ag+ Fe3+ Mg2+ Ni2+ Cu2+ Cd2+ blankFigure 1. Color change of Au-NPs in the presence of a series of metal cations.
Reference:
1. W. Chansuvarn, T. Tuntulani and A. Imyim, TrAC-Trend Anal. Chem., 2015, 65, 83-96.
2. R. Nian, D. S. Kim, T. Nguyen, L. Tan, C.-W. Kim, I.-K. Yoo and W.-S. Choe, J.
Chromatogr. A, 2010, 1217, 5940-5949.
Introduction to the author:
Dr. Ting Yang
Research Center for Analytical Sciences, Box332, Northeastern University, Shenyang
110819, China
Tel: +86-24-83687659; Fax: +86-24-83676698.
E-mail: yangting@mail.neu.edu.cn
Dr. Ting Yang was born in 1986. She got her B.Sc. in applied chemistry and PhD in
analytical chemistry from Northeastern University, China, in 2008 and 2014, respectively,
under the supervision of Prof. Jianhua Wang. In 2012-2013, she spent a year as a visiting
student in the group of Dr. Dasgupta‟s lab at UT Arlington. She is now an assistantprofessor in Northeastern University. Her research interests mainly focus on geneticallyengineered microorganisms for the purpose of heavy metal separation and analysis.

１５０

PT.09Absorption of Pb2+ and Cd2+ from Water using 3D nitrogen-doped
graphene
Ting-Ting ZHAO1, Feng LUO 2, Xin-Hong SONG3, Xi CHEN*3
1

Planning Department,Xiamen Huaxia University,Xiamen 361024,China
Fujian Research Institute of Metric Science; Fuzhou 350003, China
2
Quanzhou Medical College, Quanzhou, 362010
2

3

Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis &Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University,
Xiamen 361005,China
Corresponding author email: xichen@xmu.edu.cn
Abstract:
We present an approach for the preparation of 3D nitrogen-doped grapheme (3D rGO-DA)
using dopamine (DA) as both reductant and functionalization agents. Compared with
reduced graphene oxide (rGO), the 3D rGO-DA prescents excellent absorption ability
towards Pb2+ and Cd2+. The adsorption capacity of Pb2+ and Cd2+ by 3D rGO-DA is foundto be 75 and 65.8 mg/g, receptively. The effect factors, including adsorption time, pH andinitial concentration of Pb2+ and Cd2+, are studied. Adsorption time of 120 min and pH 5.0
are found to be the suitable condition for Pb2+ and Cd2+ adsorption. After 3 cycle
absorption/desorption, the 3D rGO-DA still remained excellent absorptive capacity andcan be easily removed from water after adsorption process, indicating its applicability inthe water purification
Keywords: 3D graphene; Pb2+; Cd2+;absorption

Fig. 1 Photographs of GO solution (A)；3D rGO-DA before lyophilization (B)；Measurement of 3D rGO after lyophilization (C)；Measurement of 3D rGO-DA after
lyophilization (D)１５１Fig. 2 SEM images of cellular structure of 3D rGO-DA shown in (A) and (B)Fig. 3 Effects of adsorption time on the adsorption capacities of 3DrGO-DA on Pb2+ andCd2+

Reference:[1]Song X H, Lin L P, Rong M C, Wang Y R, Xie Z X, Chen X. Carbon, 2014, 80: 174182[2]Sun H, Xu Z, Gao C. J. Adv. Mater., 2013, 25 (18): 2554-2560[3] Hu H, Zhao Z, Wan W, Gogotsi Y, Qiu J. J. Adv. Mater., 2013, 25 (15): 2219-2223[4] Chen W, Li S , Chen C , Yan L. J. Adv. Mater., 2011, 23(47): 5679-5683

１５２

Ting-Ting Zhao
Planning Department, Xiamen Huaxia University, Xiamen 361024,China
Tel/Fax: +86(592) 6276251
E-mail: ztt@hxxy.edu.cn
Ting-Ting Zhao graduated from Harbin Normal University with a Bachelor‟s degree ofchemistry in 2010, she entered Fuzhou University and obtained her Master‟s degree three
years later. Then she worked at Xiamen Huaxia University, she is mainly engaged inenvironmental water quality detection and purification materials research. She has applied
for a project of Xiamen Science and Technology Program in 2014 and published 6
scientific research articles related to the project.

１５３

PT.10Online multichannel ultrasonic extraction for high throughput
determination of arsenic in soil by hydride generation atomic
fluorescence spectrometry
Yuefen Zhang, a Lan Wu, a,b Chengbin Zheng,a Xiandeng Hou, a,b and Kailai Xu*aa

Key Laboratory of Green Chemistry & Technology, Ministry of Education, College ofChemistry, Sichuan University, Chengdu, Sichuan 610064, China;b
Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan 610064, China*
Corresponding author. Tel. & Fax: (+) 86-028-85412290; E-mail: xukailai@scu.edu.cn.
Hydride generation (HG) is a widely used sample introduction methodology for thedetermination of traditional hydride forming elements and several transition and noble
metals by coupling with atomic spectrometric methods. HG has advantages of highreaction yield and small matrix interference. However, when analyzing solid samples suchas soil, a tedious and time-consuming procedure for digestion and/or extraction isfrequently required, which usually needs hash conditions such as the use of concentrated
acids, high temperatures and high pressures. To overcome the disadvantages of
conventional sample pre-treatment techniques, there is an urgent need to develop new
methods and strategies. Online multi-channel ultrasonic extraction coupling with hydride
generation can greatly reduce the consumption of the sample and analyte loss, shorten theanalysis time, and improve the detection sensitivity and accuracy.
A new analytical method was developed to determine trace arsenic in soil by coupling
sequential injection multichannel ultrasonic extraction and online pre-reduction withslurry hydride generation atomic fluorescence spectrometry. Significantly enhanced
sampling throughput, reduced sample and reagent consumption, and minimized potential
contamination and analyte loss were achieved. Slurry samples spiked with different
concentrations of As(III) and hydrochloric acid were pumped into the ultrasonic extraction
chamber for 20-minute irradiation to efficiently extract As(III), which were subsequently
merged with a mixture of 1% (m/v) thiourea and 0.5% (m/v) ascorbic acid to pre-reduce
As(V) species to As(III), followed by the generation of AsH3 via using KBH4. Upon
optimization of experimental parameters, limits of detection (LODs) ranging from 0.03 to
0.07 mg kg-1 were obtained, and the precisions (RSDs) of this method were better than
2.0%. The proposed method was used for the determination of trace arsenic in several
Certified Reference Soil samples, with the obtained results in excellent agreement with
１５４

certified values based on a simple and fast standard addition method.
Key words: multichannel, ultrasonic extraction, soil, arsenic

Figure 1. Schematic diagram of the experimental setup
References
1 R. E. Sturgeon and Z. Mester, Appl. Spectrosc., 2002, 56, 202A–213A.
2 P. Wu, L. He, C. B. Zheng, X. D. Hou and R. E. Sturgeon,J. Anal. At. Spectrom., 2010,
25, 1217.
3 Z. Long, Y. M. Luo, C. B. Zheng, P. C. Deng and X. D. Hou, Appl. Spectrosc. Rev.,
2012, 47, 382–413.
4 C. B. Zheng, R. E. Sturgeon and X. D. Hou, J. Anal. At. Spectrom., 2010, 25, 1159–1165.
5 V. N. Epov, K. Benkhedda and R. D. Evans, J. Anal. At. Spectrom., 2005, 20, 990–992.
6 C. B. Zheng, L. Yang, R. E. Sturgeon and X. D. Hou, Anal. Chem., 2010, 82, 3899–3904.
7 D. Y. Deng, J. R. Zhou, X. Ai, L. Yang, X. D. Hou and C. B. Zheng, J. Anal. At.
Spectrom., 2012, 27, 270–275.
8 M. Tobiszewski, A. Mechli´nska, B. Zygmunt and J. Namies´snik, TrAC, Trends Anal.
Chem., 2009, 28, 943–951.
9 L. H. Keith, L. U. Gron and J. L. Young, Chem. Rev., 2007, 107, 2695–2708.
10 C. Bendicho, I. De La Calle, F. Pena, M. Costas, N. Cabaleiro and I. Lavilla, TrAC,
Trends Anal. Chem., 2012, 31, 50–60.
11 L. Chen, D. Song, Y. Tian, L. Ding, A. Yu and H. Zhang, TrAC, Trends Anal. Chem.,
2008, 27, 151–159.
12 M. D'Amato, F. Aureli, S. Ciardullo, A. Raggi and F. Cubadda, J. Anal. At. Spectrom.,
2011, 26, 207–213.
１５５

PT.11
Label-free detection of microRNA by Au nanoparticles decorated
reduced graphene oxide field-effect transistor biosensor
Bingjie Cai, Guo-Jun Zhang *School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065,
China
Abstract: A novel method of highly sensitive and selective detection of microRNA(miRNA) is always desirable. We have ever constructed a field effect transistor (FET)biosensor based on chemically reduced graphene oxide (R-GO) for ultrasensitive, labelfree and specific detection of peptide nucleic acid （PNA）-DNA hybridization. In thepresent presentation, we report a Au nanoparticle decorated R-GO FET biosensor forlabel-free detection of miRNA via PNA-miRNA hybridization. R-GO was prepared by
reduction of GO with 98% hydrazine, and the FET biosensor was fabricated by dropcasting the R-GO suspension onto the sensor surface, followed by exposing the R-GOassembled sensor surface to HAuCl4 solutions to achieve Au nanoparticle decoration.
After that, PNA as the capture probe was immobilized on the Au nanoparticle surface, andlabel-free detection of microRNA was successfully performed through PNA-miRNAhybridization by the R-GO FET biosensor. The proposed graphene-based FET biosensor is
expected to be a new strategy for miRNA detection.
Scope of the symposium: Nano-bioanalytical chemistry.
Keywords: Graphene, field effect transistor biosensor, miRNA detection
Reference:
1. Cai, B.; Wang, S.; Huang, L.; et al., ACS Nano. 2014, 8, 2632-2638.
2. Cai, B.; Huang, L.; Zhang, H.; et al., Biosensors and Bioelectronics, 2015, 74, 329-334.
Introduction to the author:
Professor Dr. Guojun Zhang
School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065,
China
Tel: +86-27-68890259; Fax: +86-27-68890259.
E-mail: gjzhangtj@hotmail.com
１５６

Biography of the authors.
Guo-Jun Zhang got a PhD degree in Medicine from the Tongji Medical School of
Huazhong University of Science and Technology, P. R. China, in 1999. From 1999 to 2004,
he was a Postdoctoral Fellow at Wuhan University of P. R. China, Institute for PhysicalHigh Technology Jena of Germany, Waseda University of Japan, respectively. Afterwards,
he became a Visiting Lecturer in 2004, a Visiting Associate Professor in 2005 at Waseda
University, Japan, and Member of Technical Staff at Institute of Microelectronics,
Singapore (2006-2011), respectively. He is currently working as a professor at Hubei
University of Chinese Medicine, China.

１５７

PT.12
GC/MS analysis and sensory evaluation of odor compounds inenvironmental tobacco smoke (ETS)
Hiroshi Sato1)*, Masahiro Oka1), Shuhei Nagahashi1), Kaori Imi1), Naoki Mizoguchi1),
Hidetaka Matsubara2), Miyuki Noguchi3), Keiichi Arashidani4)1) Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki International
University
2) Chuken Laboratory for Life and Environment3) Faculty of Science and Technology, Seikei University
4) School of Health Sciences, University of Occupational and Environmental Health
Abstract:
Background:
The details on how the various compounds of tobacco smoke contribute to tobacco odor
are not yet clearly defined. Elucidation of those details may make it possible to evaluate
the ETS exposure level from the point of view of tobacco odor levels. This study is
focused on analyzing the elements of tobacco smoke at the detailed level with
instrumental analysis and sensory evaluation and verifying the data by reproducing the
tobacco odor using commercial reagents.
Methods:
1. GC/MS and quantitative analysis of tobacco smoke and tar and reproduction of tobaccoodor with commercial reagents.
2. Preparation and sensory evaluation of imitation tobacco with Hibiscus hamabo leaves
and nicotine solution.
3. Analysis of tobacco tar on wall surface and its change of properties with time
4. GC analysis and sensory evaluation of residual chemicals left on indoor surfaces by
tobacco smoke (thirdhand smoke)
Result:Compounds including nicotine, phenol and acetic acid were detected with GC/MS
analysis from both tobacco smoke (Table 1) and tar. From the data obtained with sensory
evaluation of imitation tobacco, nicotine was thought to be the constituent of tobacco odor;
however, its behavior in the environment is fairly unstable. In the attempt to reproduce
tobacco odor with commercial reagents, nicotine itself did not have any detectable smell.
１５８

Table 1 Concentration and odor intensity of secondhand smoke
Odor
Concentration Threshold
Compounds
intensity* Quality of odor
(v/vppm)
(ppm)
(1~5)
Ammonia

50

1.5

1.5

Irritant odor

Nicotine

0.5

0.011

1.7

Pyridine
odor

like

Acetaldehyde 8.90.0015

3.8

Irritantodor

grassyAcetic acid0.5

0.0060

2.0

Irritant
odor

acidicPhenol0.5

0.00562.0

Chemical odor

*Odor intensity＝log(concentration/threshold)Conclusion:
According to the result of this study, not the nicotine but the pyrolysis products of nicotine
may be contributing to the unpleasant quality of tobacco odor. The change of theproperties of nicotine and third hand smoke with time are to be further examined.
Keywords: ETS, secondhand smoke, thirdhand smoke
Reference:
1. Ena Sonoda et. al., Development of the measuring method of odor compounds inenvironmental tobacco smoke (ETS), pp9-10, Proceedings of Annual meeting of theSociety of Indoor Environment, Kyushu Branch, 2014
2. Masahiro Oka et. al., Analysis of environmental tobacco smoke (ETS), pp132-133,
Proceedings of Annual meeting of the Society of Indoor Environment, 2014
3. Shuhei Nagahashi et. al., Study on environmental tobacco smoke (ETS), pp27-28,
Proceedings of Annual meeting of the Society of Indoor Environment, Kyushu Branch,2015
4. Hiroshi Sato, Keiichi Arashidani, Development of the measuring method of odor
compounds in environmental tobacco smoke (ETS) and its application to environment,
pp811-814, Smoking Research Foundation annual research report 2014 (2015)
Acknowledgements:
This work was supported by a grant from The Smoking Research Foundation.

１５９

Introduction to the author:Dr. Hiroshi Sato
Faculty of Pharmaceutical Sciences, Department of Pharmacy, Nagasaki International
University
2825-7, Huis Ten Bosch, Sasebo City, Nagasaki 859-3298 Japan
Tel: +81-956-20-5668; Fax: +81-956-20-5668
E-mail: satoh@niu.ac.jp
Dr. Hiroshi Sato received his Master degree in 1983 and received a PhD degree in
Pharmaceutical Sciences (2003) at Kyushu University in Japan. He had worked in Kao
Corporation during 1985-1987 and then had worked in TOTO LTD. during 1987-2006. He
is currently an associate professor of Nagasaki International University. His research is
focused on the environmental analysis and bioremediation.

１６０

PT.13
Application of HPLC by Using Mass Spectrometry Detector
Masaki Watanabe*, Masaki Yoshie, Norimasa Minamoto, Kousaku Toyosaki
Optical Instrument Engineering Department, Hitachi high-tech Science Corporation, 1040
Ichige, Hitachinaka, Ibaraki 312-0033 Japan
Abstract: Mass spectra data is quite informative for HPLC analysis. It can confirm
compound identities, can clarify co-elutions of mixed compounds, and can detectcompounds which do not have UV absorbance or fluorescence. Here we introduce a newcompact mass detector equipped the atmospheric pressure ion filter technology. The
atmospheric pressure ion filter is a new selective ion transfer technology, which provide
selective ion detection and the robustness of routine operations. In this presentation, we
introduce applications of the new mass detector combined with HPLC.
Keywords: LC/MS, mass detection
Introduction to the author:
Dr. Masaki Watanabe
Optical Instrument Department, Hitachi High-Tech Science Corporation, Hitachinaka,Ibaraki, 312-0033 Japan
Tel: +81-50-31733080; Fax: +81-29-2757099.
E-mail: masaki.watanabe.yk@hitachi-hightech.com
Biography of the authors.
Dr. Masaki Watanabe is an application scientist and an engineer of Optical Instrument
Engineering Department, Hitachi high-tech Science Corporation. He received his Ph.D. ofengineering from Nagoya University in 2002 and then joined Hitachi High Technologies.
He is in charge of the development of application system of HPLC and LC/MS.

１６１

PT.14
Analytical method for arsenic speciation in foods using HPLC-ICP/MS
Dongwon Seo*, Soyoung Kim, Eunjoo Park, Jisu Park, Jaeho Ha, Tae-Gyu Nam,
Jooyoung Lee, Haewon Jang, Jinbong Hwang
Food Analysis Center, Korea Food Research Institute, Seongnam 463-746, Korea
Abstract: Chemical species of elements in biological and environmental samples are ofimportance to understand toxicity, metabolism and transport properties of elements.
Therefore, speciation analysis becomes an increasing active-research field in recent years.
Different atomic spectrometric methods were applied for As speciation in foods, such as
high performance liquid chromatography (HPLC) coupled with inductively coupledplasma mass spectrometry (ICP-MS). In this study, the analytical method to determine fivekinds of arsenic speciation; the inorganic arsenic species As(III), As(V) and the organic
species arsenobetaine (AsB), dimethyl arsinate (DMA) and monomethyl arsenate (MMA)
were determined in foods by HPLC-ICP/MS method. The ICP/MS operating conditions
were 1500W RF power, 15 L/min plasma Ar gas flow, 0.95 L/min nebulizer gas flow, 1.2
L/min Aux. gas flow, 1.5 mL/min sample uptake rate and As ion measured were 75 m/z.
The calibration curve showed linearity in the range between 0.9991~0.9995. Limits ofdetection (LOD) in As(III), As(V), AsB, DMA and MMA were measured 1.29, 0.19, 0.20,
0.15 and 0.36 ng/mL and limits of quantification (LOQ) were 4.31, 0.64, 0.66, 0.51 and1.19 ng/mL, respectively. Intra-day for As(III), As(V), AsB, DMA and MMA were 2.74,
1.05, 1.52, 2.44 and 1.25 %, and inter-day were 6.87, 8.51, 6.10, 6.76 and 6.91 %,respectively.
Keywords: HPLC-ICP/MS, Inorganic arsenic species, Organic arsenic species, Foods
Reference:
1. Comparison of different medical cases in urinary arsenic speciation by fast HPLCICP/MS, P. Heitland and H. D. Koster, Int. J. Hyg. Environ. Health 212 (2009) 432–438
2. Study on simultaneous speciation of arsenic and antimony by HPLC-ICP/MS, Y.
Morita, T. Kobayashi, T. Kuroiwa and T. Narukawa, Talanta 73 (2007) 81–86
Introduction to the author:
Dongwon Seo
Food Analysis Center, Korea Food Research Institute, 463-746, Korea
１６２

Tel: +82-31-780-9241; Fax: +82-31-780-9280.
E-mail: dwseo@kfri.re.kr
Biography of the authors:
Dongwon Seo joined the Food Analysis Center at Korea Food Research Institute in 2003,
where he involved himself in the analysis of food components, including mineral nutrientsand heavy metal using ICP/MS. Concurrently, he is applying GCxGC-TOFMS technology
coupled with silver ion cartridge techniques for the analysis of trans fatty acids in foods.
His research and activity is focused on chromatographic separation, food analysis.

１６３

PT.15
Nutritional composition of bamboo shoots (Phyllostachys Bambusoides
Sieb. et Zucc.) in Korea
Dongwon Seo1*, Songjin Lee2, Jinbong Hwang1, Kinjin Kim1, Soyoung Kim1, Eunjoo
Park1, Hyunjun Lee1, Haebin Cho1, Seunghee Kim1, Wonjin Yoon1, Jaeho Ha1
1
2

Food Analysis Center, Korea Food Research Institute, Seongnam 13539, Korea
Bamboo Resource Research Institute, Damyang 57365, Korea

Abstract: Bamboo shoots are of considerable nutritional value to human health since they
are rich in functional nutrients like dietary fiber, protein, vitamins, bioactive compounds
and low in fat and calories. Apart from the Asian countries such as China, Japan, andKorean, where it is frequently used as common food material, bamboo shoots havebecome very popular in many other countries where bamboo shoots are mostly imported.
We assessed the quality of a bamboo shoots (Phyllostachys Bambusoides Sieb. et Zucc.)
produced in Damyang-gun in terms of parameters that included fat, protein, carbohydrate,
dietary fiber, mineral contents and fatty acids. The moisture, fat, protein, ash,carbohydrate and dietary fiber of the bamboo shoots were 92.1, 0.4, 2.4, 0.8, 4.3 and 1.8
g/100 g, respectively. Mineral contents of Ca, Fe, K, Mg, Na, P and Zn were 8.3, 0.9,
369.6, 13.1, 23.9, 53.6 and 0.8 mg/100g, respectively. Fatty acids composition (percentage
results from 100g fat) of palmitic acid, stearic acid, oleic acid, linoleic acid and linolenicacid were 35.3, 1.5, 8.6, 38.9 and 9.5 %, respectively.
Keywords: Bamboo shoot, Nutritional composition, mineral content, fatty acid
Reference:
1. Gamma radiation control quality and lignification of bamboo shoots (Phyllostachys
praecox f. prevernalis.) stored at low temperature, F. Zeng, Z. Luo *, J. Xie, S. Feng,
Postharvest Biology and Technology 102 (2015) 17–24
2. Bamboo shoot processing: food quality and safety aspect (a review), S. Satya, L.M. Bal,
P. Singhal , S.N. Naik, Trends in Food Science & Technology 21 (2010) 181-189
3. Chemical Characteristics and Flavors of Bamboo-shoot Vinegar, H. Jang, D. Seo, J.
Hwang, S. Lee, J. Ha, Korean J. Food Sci. Technol. 45 (2013) 675-681
Introduction to the author:
１６４

Dongwon Seo
Food Analysis Center, Korea Food Research Institute, 463-746, Korea
Tel: +82-31-780-9241; Fax: +82-31-780-9280.
E-mail: dwseo@kfri.re.kr
Biography of the authors:
Dongwon Seo joined the Food Analysis Center at Korea Food Research Institute in 2003,
where he involved himself in the analysis of food components, including mineral nutrientsand heavy metal using ICP/MS. Concurrently, he is applying GCxGC-TOFMS technology
coupled with silver ion cartridge techniques for the analysis of trans fatty acids in foods.
His research and activity is focused on chromatographic separation, food analysis.

１６５

PT.16Comparison of Macro Mineral Contents following Kind of Vegetables
produced in Korea
Eun-joo Park1* , Soyoung Kim1, Jinbong Hwang1, Dongwon Seo1, Youngmin Choi2, SeNa Kim2, Jin-Sik Nam3
1
2

Department of Food analysis, Korea Food Research Institute, Gyeonggi, 13539, Korea,
Functional Food & Nutrition Division, National Academy of Agricultural Science, RDA,

Jeonju, 04535, Korea
3
Department of Food & Nutrition, Suwon Women's University, Gyeonggi, 18333, Korea
Abstract:
Recently, macro minerals (Na, Ca, K Mg and P) have the excellent functions associatedwith prevention of disease. We estimated an amount of macro minerals in the vegetables
produced in Korea in order to build the food nutritional ingredient table. A SRM 1849apurchased from NIST in USA was digested by the microwave and validation of macro
minerals in SRM 1849a was carried out by inductively coupled plasma optical emission
spectrometer (ICP-OES). Regression analysis of correlation coefficient (R2) was higher
than 0.999 which revealed good linearity. Based on this analysis, the raw vegetables
contained 0.20 to 144.98 mg/100g of sodium, 7.81 to 266.57 mg/100g of calcium, 230.57
to 812.73 mg/100g of potassium, 12.16 to 76.28 mg/100g of magnesium and phosphorus
ranged from 35.32 to 123.81 mg/100g. In addition, the contents of the minerals in the
blanched vegetables were 1.40 to 134.75 mg/100g of sodium, 7.80 to 177.58 mg/100g of
calcium, 150.32 to 651.71 mg/100g of potassium, 11.17 to 56.63 mg/100g of magnesium
and 36.20 to 136.61 mg/100g of phosphorus. Hence, it would be expected that the mineralcontents could be used to draw up the food nutritional ingredient table.
Keywords:

ICP-OES, Mineral, Food composition table, vegetables

Reference:
1. Korea Food and Drug Administration (2009) Korea Food Code,Korea Food Industry
Association, Seoul, Korea
2. Official Methods of Analysis (2005) 18th Ed., AOAC INTERNATIONAL,
Gaithersburg, MD, Method 992.06

１６６

Introduction to the author:
Dr. Jinbong Hwang
Food Analysis Center, Korea Food Research Institute, 13539, Korea
Tel: +82-31-780-9128; Fax: +82-31-780-9280.
E-mail: Hwangjbg@kfri.re.kr

１６７

PT.17Single molecule real-time imaging of intracellular Mg2+ signaling andwave propagation in single cell with subdiffraction-limit resolution
Suresh Kumar Chakkarapania, Seungah Leeb, and Seong Ho Kanga,b,*a

Department of Chemistry, Graduate School, Kyung Hee University
Department of Applied Chemistry, College of Applied Science, Kyung Hee Universityb

Abstract:
Intercellular magnesium ion (Mg2+) waves are believed to propagate through networks ofhuman cells by the release of ATP, which functions as an extracellular messenger.
However, the intracellular location of the Mg2+ signaling and dynamics of the wave
propagation are not completely studied at the single cell level. To study the intracellular
Mg2+ signaling with subdiffraction-limit resolution by dSTORM, a photo-switchable
intracellular magnesium fluorescent indicator dye is required. In this work, mag-fluo-4AM an intracellular Mg2+ fluorescent indicator dye was photo-switched between
fluorescent state and dark state by an activation and excitation laser. By localizing thecentroid of the fluorophores, intracellular Mg2+ was located in intracellular region withsubdiffraction-limit resolution. To investigate the intracellular Mg2+ wave propagation,
Na+ATP was added alongside mag-fluo-4-AM with the above-mentioned photo-switchingcondition. Intracellular magnesium wave propagation was investigated at 0.2 s temporal
resolution with 21  1 nm spatial resolution. This study provides the proof-of-concept for
the use of dSTORM to investigate the changes in intracellular ions with subdiffraction
limit spatial resolution and a lower temporal resolution than a conventional fluorescencemicroscope.
Keywords: intracellular Mg2+, subdiffraction-limit resolution, real-time investigation
Reference:
1. S. Lee, H. G. Lee, S. H. Kang, Anal. Chem. 2009, 81, 538.
2. M. Heilemann, S. van de Linde, A. Mukherjee, and M. Sauer, Angew. Chem. Int. Ed.
Engl. 2009, 48, 6903.
Introduction to the author:
Professor Dr. Seong Ho Kang
１６８

Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446701, Republic of Korea
Tel: +82-31-201-3349; Fax: +82-31-201-2340.
E-mail: shkang@khu.ac.kr
Biography of the authors.
Suresh Kumar Chakkarapani received his B.Sc and M.Sc in chemistry from Madras
University of India in 2009 and 2011. He worked as an assistant professor in Ramakrishna
Mission Vivekananda College affiliated to Madras University from 2011 to 2014. He iscurrently a doctoral student at Kyung Hee University from 2014. His research interests
include single molecular imaging and development of various analytical techniques for
micro- and nano-separation.
Seungah Lee received her B.S., M.S., and Ph.D. from Chonbuk National University of
Republic of Korea in the group of Professor Seong Ho Kang in 2003, 2006, and 2010,
respectively. She is currently an Assistant Professor at Kyung Hee University. Her main
research interests are chemical/biological analysis on biochip at single-molecule level
using various detection systems.
Professor, Seong Ho Kang received his B.S. and M.S. degrees in chemistry from Kyung
Hee University of Republic of Korea in 1987 and 1989. He worked as a senior researcher
at the research center of Hanil Pharmaceutical Co. (present, CJ Pharm. Co.) from 1989 to
1997. He received his Ph.D. degree in chemistry from the Seoul National University in
1998. He was a postdoctoral research associate scholar at Iowa State University and Ames
Laboratory-US Department of Energy with Dr. Edward S. Yeung from 1998 to 2000, asenior researcher at the SAIT (Samsung Advanced Institute of Science and Technology) in
2000–2002, and a professor of chemistry at Chonbuk National University in 2002–2010.
He is currently a professor at Kyung Hee University from 2010. His research interests
include developments of various analytical systems, methods, and techniques based on
micro- and nano-separation, single-molecule detection, micro- and nano-fluidics, andvarious nano-bio fusion technologies.

１６９

PT.18Novel detecting method of synthetic organic dyes by dual non-aqueous
capillary electrophoresis
Moonhee Parka, Seung-Hoon Bahangb, Seong Ho Kanga,c,*a

Department of Chemistry, Graduate School, Kyung Hee University
Criminal Investigation Command of MND, Seoul 140-701, Republic of Koreac
Department of Applied Chemistry, College of Applied Science, Kyung Hee Universityb

Abstract:
Many researchers have been conducted to use a variety of devices to detect the syntheticorganic dyes. A novel dual non-aqueous capillary electrophoresis (d-NACE) with laserinduced fluorescence (LIF) system was developed for simultaneous analysis of syntheticorganic dyes. NACE was provided faster analysis time, lower sample consumption, andhigher solubility for synthetic organic dye analysis former conventional aqueous CE
techniques. In addition to NACE, two lasers and detectors were used along with a single
capillary. Dual-wavelength excitation light sources were used to excite different organic dyes [i.e.,
543 nm for crystal violet (CV), methyl violet B (MVB), methyl violet B base (MBB),
rhodamine 6G (R6G), and rhodamine B base (RBB); 635 nm for nile blue A (NBA) andmethylene blue (MB)]. The dyes were analyzed in 15 min with an excellent baseline separation(resolutions ≥ 2.6) under the electric field of 500 V/cm. The detection limits (S/N = 3)
were 0.1 mM-40 fM, which were 10-105 times lower than the previous detection methods.
Hence, d-NACE method was proved to be high-throughput and effectively rapid analysis
technique for the highly sensitive detection of synthetic organic dyes.
Keywords: dual non-aqueous capillary electrophoresis, organic dyes, high-throughput
detecting
Reference:
1. E. Kenndler, J. Chromatogr. A 2014, 1335, 16-30.
2. M. Calcerrada, C. García-Ruiz, Anal. Chim. Acta 2015, 853, 143-166.
Introduction to the author:
Professor Dr. Seong Ho Kang
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446１７０

701, Republic of Korea
Tel: +82-31-201-3349; Fax: +82-31-201-2340.
E-mail: shkang@khu.ac.kr
Biography of the authors.
Moonhee Park received her B.S in chemistry from Kyonggi University of Korea in 2014.
She is currently a master student at Kyung Hee University from 2014. Her researchinterests Capillary electrophoresis with non-aqueous solvent and development of various
analytical techniques for separation.
Seung-Hoon Bahang received his B.S. and M.S. degrees in chemistry from Kyung Hee
University of Republic of Korea. He is currently a section chief at Criminal Investigation
Command of MND. His research interests include developments of various analyticalsystems, methods, and techniques based on micro- and nano-separation.
Professor, Seong Ho Kang received his B.S. and M.S. degrees in chemistry from Kyung
Hee University of Republic of Korea in 1987 and 1989. He worked as a senior researcher
at the research center of Hanil Pharmaceutical Co. (present, CJ Pharm. Co.) from 1989 to
1997. He received his Ph.D. degree in chemistry from the Seoul National University in
1998. He was a postdoctoral research associate scholar at Iowa State University and Ames
Laboratory-US Department of Energy with Dr. Edward S. Yeung from 1998 to 2000, asenior researcher at the SAIT (Samsung Advanced Institute of Science and Technology) in
2000–2002, and a professor of chemistry at Chonbuk National University in 2002–2010.
He is currently a professor at Kyung Hee University from 2010. His research interests
include developments of various analytical systems, methods, and techniques based on
micro- and nano-separation, single-molecule detection, micro- and nano-fluidics, andvarious nano-bio fusion technologies.

１７１

PT.19Biomolecular quantification in axial nanoscale of plasmonic particle on
nanoisland
Seungah Leea, Sujin Ahnb, Seong Ho Kanga,b,*a
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446701, Republic of Koreab
Department of Chemistry, Graduate School, Kyung Hee University, Yongin-si, Gyeonggido 446-701, Republic of Korea
Abstract: A spatial localization of biomolecule based on the plasmonic scatttering of sliver
nanoparticle on the gold nanoisland was investigated by using polarization-sensitive totalinternal reflection scattering (TIRS) imaging. Immunoassay through TIRS technique basedon reconstructing the three dimension (3D), indeed provides the best possible accuracy byusing z-stage controller in the spatial range up to 10 nm in the z-direction. By measuring
the scattering of the sliver nanoparticle through sandwich immunoreaction on gold
nanoisland, we verified 3D resolution (spatial localization) of single biomolecule withhigh accuracy. In addition, it offers a good platform for sensitive quantitation with thelimit of detection of 800 zeptomolar (zM, 10-21 M) level of protein maker.
Keywords: total internal reflection scattering, zeptomolar concentration, axial nanoscale
Reference:
1. S. Lee, S. H. Kang, Analyst, 2013, 138, 3478.
2. S. Lee, S. H. Kang, Talanta, 2013, 104, 32.
3. S. Lee, S. H. Kang, Biosens. Bioelectron. 2014, 60, 45.
4. S. Lee, H. Yu, S. H. Kang, Chem. Commun., 2015, 51, 945.
Introduction to the author:
Professor Dr. Seong Ho Kang
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446701, Republic of Korea
Tel: +82-31-201-3349; Fax: +82-31-201-2340
E-mail: shkang@khu.ac.kr
Biography of the authors.
１７２

Seungah Lee received her B.S., M.S., and Ph.D. degrees in chemistry from Chonbuk
National University of Republic of Korea in 2003, 2006, and 2010, respectively. She iscurrently an Assistant Professor at Kyung Hee University. Her main research interests are
chemical/biological analysis on biochip at single-molecule level using various detection
systems.
Sujin Ahn received her B.S. degree in Food Science and Biotechnology from Kyung Hee
University of Republic of Korea in 2014. She is currently a researcher in department ofchemistry at Kyung Hee University from 2015. Her research interest includes single
molecule detection and analysis of biological species by fluorescence and fluorescencefree microscopic imaging.
Seong Ho Kang received his B.S. and M.S. degrees in chemistry from Kyung Hee
University of Republic of Korea in 1987 and 1989. He worked as a senior researcher at the
research center of Hanil Pharmaceutical Co. (present, CJ Pharm. Co.) from 1989 to 1997.
He received his Ph.D. degree in chemistry from the Seoul National University in 1998. He
was a postdoctoral research associate scholar at Iowa State University and Ames
Laboratory-US Department of Energy with Dr. Edward S. Yeung from 1998 to 2000, asenior researcher at the SAIT (Samsung Advanced Institute of Science and Technology) in
2000-2002, and a professor of chemistry at Chonbuk National University in 2002-2010.
He is currently a professor at Kyung Hee University from 2010. His research interests
include developments of various analytical systems, methods, and techniques based on
micro- and nano-separation, single-molecule detection, micro- and nano-fluidics, andvarious nano-bio fusion technologies.

１７３

PT.20Time-Series Variation of Atmospheric Radon-222 Concentrations at
Gosan Site in Jeju Island of Korea during 2012-2014
Jung-Min Song1, Jun-Oh Bu1, Won-Hyung Kim1, Chang-Hee Kang1*, S. Chambers2
1
2

Division of Chemistry and Cosmetics, Jeju National University, Jeju 690-756, Korea
Australian Nuclear Science and Technology Organisation, NSW 2232, Australia

Abstract: Radon-222, a radioactive noble gas, is released into the atmosphere from soils
over the continents and has a half-life of 3.8 days. Because radon fluxes from soils are
typically 100 times compared to those from the ocean, radon is considered as a usefultracer for air masses which have passed over land recently. The measurement of
atmospheric radon-222 concentrations at a background area is used to determine whether
the air masses originate from over continents or oceans. Further, it is an excellent tracer
for studying vertical mixing of greenhouse gases, boundary-layer development anddynamics, and also for validation of three-dimensional atmospheric transport models. In
conjunction with other radionuclide measurements, such as 210Pb, radon data provide a
useful constraint for the evaluation of air transport models and the identification of global
atmospheric conditions. In this study, the ambient background level of atmospheric radonat Gosan Site in Jeju Island of Korea was measured using a real-time monitoring systemthroughout 2012-2014, in order to evaluate the timely variation characteristics as well asbackground levels of atmospheric radon. The annual mean radon concentration was
2,254±1,127 mBq/m3 during the studying period. In seasonal comparison, the order of themean concentrations was as winter (2,734±1,124 mBq/m3) > fall (2,686±1,026 mBq/m3) >spring (2,063±911 mBq/m3) > summer (1,639±1,116 mBq/m3), showing relatively high in
winter and low in summer season. The monthly mean concentrations were in the order of
Nov > Jan > Oct > Dec > Sep > Feb > Mar > May > Jun > Apr > Aug > Jul, resulting theconcentration on November (2,839 mBq/m3) was more than twice as that on July (1,353
mBq/m3). Meanwhile the hourly concentrations have increased during the nighttime(2,617 mBq/m3 at around 7am), and decreased relatively during the daytime (1,874
mBq/m3 at around 3pm). Compared to other foreign regions, the mean radon
concentration at Gosan Site was 2.5 times lower than that at Hok Tsui of Hong Kong, but
22.1 times higher than that at Mauna Loa of Hawaii. Based on the back trajectory analysis,
the frequencies of air mass moving from China continent, the Korean peninsula, the East
Sea and the North Pacific Ocean were 50.4, 18.6, 18.9, 12.1%, respectively, and especially
１７４

the ambient radon concentration was quite high (2,587 mBq/m3) by the air masses movingfrom China continent. On the study of continental fetch analysis, the radon concentrations
were high when the air masses moved from the Asia continent to Jeju Island, on the other
hand, those values were low when they moved from the North Pacific Ocean.
Keywords: Radon-222, Atmospheric radon, Background level, Gosan Site
Reference:
1. Chamber, S.D., F. Wang, A.G. Williams, D. Xiaodong, H. Zhang, G. Lonati, J.
Crawford, A.D. Griffiths, A. Ianniello, and I. Allegrini, Atmospheric Environment, 2015,
107, 233-243.
2. Kim, W.H., H.J. Ko, C.G. Hu, H.Y. Lee, C.K. Lee, S. Chamber, A. G. Williams, andC.H. Kang, Bull. Korean Chem. Soc., 2014, 35(4), 1149-1153.
Introduction to the author:
Dr. Chang-Hee Kang
Division of Chemistry and Cosmetics, Jeju National University, Korea
Tel: +82-64-754-3545; Fax: +82-64-756-3561
E-mail: changhee@jejunu.ac.kr
Biography of the authors.
Jung-Min Song has obtained Ph.D. degree in 2014 and is working as a Postdoctoral
researcher in Division of Chemistry and Cosmetics, Jeju National University. Hisscientific research interests are focused on the environmental analysis for the air pollutants
such as Radon-222, odorous compounds and atmospheric aerosols.

１７５

PT.21
Effect of Asian Dust Storms on Atmospheric Aerosol Compositions in
Background Site of Korea during 2012-2014
Jun-Oh Bu1, Jung-Min Song1, Jae-Yun Lee1, Jong-Heon Han1, Chang-Hee Kang1*
1
Division of Chemistry and Cosmetics, Jeju National University, Jeju 690-756, Korea
Abstract: The collection of atmospheric PM10 and PM2.5 aerosol samples has been made atGosan Site of Jeju Island, which is one of the background sites of Korea, during 20122014. Their water-soluble ionic and elemental species were analyzed in order to examine
the variable characteristics of aerosol composition and pollution in relation to the Asian
dust storms. The average mass concentrations of PM10 and PM2.5 fine particulate matterswere 128.9 ± 49.0 μg/m3 and 41.5 ± 17.1 μg/m3, respectively, for the Asian dust days,
showing about 3-4 times higher than those of non-Asian dust days. The concentrations ofPM10 ionic species were in the order of nss-SO42- > NO3- > NH4+ > Cl- > Na+ > nss-Ca2+ >K+ > Mg2+, and those of PM2.5 were nss-SO42- > NH4+ > NO3- > Na+ > Cl- > K+ > nss-Ca2+> Mg2+ during the Asian dust days. Their concentrations were 1.4~8.4 times for PM10 and
1.2~4.1 times for PM2.5, during Asian dust periods, compared to those of non-Asian dustdays. Especially, the concentrations of anthropogenic and soil originated species increased
highly in both PM10 and PM2.5 aerosols during the periods of Asian dust storms. The
composition ratio comparison resulted that the secondary pollutants (NH4+, nss-SO42-,
NO3-) were 72.8%, but those of marine species (Na+, Cl-, Mg2+) and soil source (nss-Ca2+)
were 20.4% and 5.0%, respectively, for the PM10 ionic species during the Asian dust
periods. Meanwhile, the composition ratios of the secondary pollutants, marine species,
and soil source were 93.4%, 3.9%, and 0.7%, respectively, for the PM2.5 ionic species.
From the elemental analysis of atmospheric aerosols, the elemental concentrations ofPM10 were in the order of S > Al > Na > Ca > Fe > Mg > K > Ti > Zn > Mn > Pb > Ba >Ni > V > Sr > Cu > Cr > Cd > Co > Mo, and those of PM2.5 were S > Fe > Al > Na > K >Ca > Mg > Zn > Pb > Mn > Ti > V > Ni > Ba > Cu > Sr > Cr > Cd > Mo > Co. Therefore,
the anthropogenic (S, Zn, Pb), Soil (Al, Fe, Ca) and Marine (Na, Mg) species occupied
23.4%, 44.9% and 24.0% in PM10 and 47.9%, 28.5%, 14.2% in PM2.5, respectively, for the
Asian dust periods. The neutralization factors by NH3 were 0.24 in PM10 and 0.34 in PM2.5
aerosols, meanwhile those by CaCO3 were 0.09 and 0.01, respectively. The back trajectory
analysis showed that the concentrations of anthropogenic and soil species of both PM10
and PM2.5 aerosols were higher when the air mass moved from China continent to Jeju
Island rather than when it came from the North Pacific Ocean.
１７６Keywords: Asian dust, Atmospheric aerosol, Gosan Site of Jeju Island
Reference:
1. Kim, K.J., S.H. Lee, D.R. Hyeon, H.J. Ko, W.H. Kim, and C.H. Kang, Analyticalscience & Technology, 2014, 27(1), 1-10.
2. Lee, D.E., W.H. Kim, H.J. Ko, Y.S. Oh, and C.H. Kang, J. .Korean Society for
Atmospheric Environment, 2013, 29(3), 325-337.
Introduction to the author:
Dr. Chang-Hee Kang
Division of Chemistry and Cosmetics, Jeju National University, Korea
Tel: +82-64-754-3545; Fax: +82-64-756-3561
E-mail: changhee@jejunu.ac.kr
Biography of the authors.
Jung-Oh Bu has obtained Master degree in 2013 and is studying as a doctor's course inanalytical chemistry at Chemistry Department of Jeju National University. His scientificresearch interests are focused on the environmental analysis of air pollutants such asatmospheric aerosols, rainwater, Radon-222, etc.

１７７

PT.22
Verification of Applicability of the Validated Analytical Method with a
Liquid Chromatography-Tandem Mass Spectrometry for Steroids
Residues Screening in Food Products
Hwa-Mi Lee*, JaeHyun Kim, WooJin Jeon, SooJin Bang, SungMin Woo, JuYeun Lee,
EunJoo Koo, JyongEun Myung, HyunUk Kim, EunYoung Lee, Soo Jung Hu, Young-Mi
Jang, Chul Joo Lim.
Center for Food & Drug Analysis, Gyeongin Regional Food and Drug Administration,
137, Juan-Ro, Nam-Gu, Incheon-City, 402-835, Republic of Korea
Steroids, a group of cyclic organic compounds whose most common characteristic is anarrangement of seventeen carbon atoms in a four-ring structure, are allowed to be onlyused as a doctor‟s prescription to a patient for therapeutic purposes. But, the incidents of
the illegal distribution of food products contained steroid have been reported continuously
in recent years. A developed quantitative method using liquid chromatography–tandem
mass spectrometry (LC–MS–MS) by the advanced analysis team in National Institute ofFood and Drug Safety Evaluation was evaluated for the applicability of 30 steroids in foodproducts including health supplements using inter-laboratory verification method. In thisstudy, we verified by using the positive ionization mode for the analysis of steroids. The
evaluation of parameters such as specificity, linearity, precision, accuracy, and adequate
sensitivity like as a limit of detection led us to establish a sensitive new electrospray
LC/MS method for steroid analysis. LC/MS technique, together with the combined
knowledge of the LC retention behavior, the relative sensitivity, and the fragmentation
pattern generated by electrospray mass spectrometry, will be extremely useful for theidentification and detection of steroids.
Tel. +82-32-450-3351, Fax. +82-32-442-4622, E-mail. lhm9904@gmail.com

１７８

PT.23
Method development for determination of heavy metals in turmeric
powder using ICP-MS
Chang-Hee Lee, Yeon Kim, Myung-Ja Lee, Bock-Kyu Lim, Ki-Hyun Kim, Yeong-Hyeon
Kim, Tae-Yong Cho, Jae-Young Shim*
Center for Food & Drug Analysis, Busan Regional Korea Food and Drug Administration,
Busan, Republic of Korea
Turmeric, commonly known as a yellow spice, is widely consumed as curry by Korean
and less frequently consumed as a beverage in itself or added in tea or dessert soup.
Turmeric can also be contaminated some harmful matter, e.g. micotoxin and heavy metals.
In this study, the analytical method to determine heavy metals; Arsenic (As), Cadmium(Cd), Lead (Pb) and Mercury (Hg) in turmeric powder was developed using ultramicrowave and inductively coupled plasma mass spectrometry. Limits of quantificationin samples for As, Cd, Pb and Hg were measured 2.58, 0.08, 0.43, and 0.01 ug/kg,
respectively. The calibration curve showed linearity in the range between 0.998~0.999.
Recovery for As, Cd, Pb and Hg were 111, 91, 94, and 98%, respectively

１７９

PT.24
Effect of Different Biomass on Value-added Products in Bio-liquid of
Food Waste via Hydrothermal Carbonization
Soomi Park1, Jonathan Lee2, Yura Hwang1, Soyoung Lee1, Sunyoung Bae1*
Department of Chemistry, Seoul Women‟s University Nowon-Gu, Hwarangro, Seoul,
Korea, 131
Davidson College 405 North Main Street, Davidson, NC 280352
Abstract: Hydrothermal carbonization (HTC) of food biomass produced bio-liquid as a byproduct. During HTC reaction, various compounds were generated by hydrolysis,
condensation, decarboxylation, polymerization and other reactions. From our previous
experiments, a few value-added compounds from bio-liquid of food waste werecharacterized; butylated hydroxytoluene (BHT) and 5-(hydroxymethyl)2-furaldehyde (HMF). BHT shows the antioxidant activity even though it also possess the
toxicity and potential health hazard. HMF was treated as a renewable material to play animportant role in a bio-fuel production. In this study, the quantitative information of thesetwo compounds produced by different biomass was obtained. Liquid liquid extraction(LLE) method was applied using various solvents including dichloromethane mixed with
ethyl ether and methanol, respectively. The bio-liquid sample dissolved in methanol was
filtrated using a 0.2 µm syringe filter and injected into GC/FID (Hewlett Packard 5890
series Ⅱ, HP-5 capillary column, 30 m × 0.25 mm × 0.25 μm). To obtain qualitative
information, one point standard addition method was chosen to correct matrix effect. The
results are expected to show the effect of complexity occurred by different biomass on theamount of value-added products.

Keywords: Biomass, Hydrothermal carbonization, Bio-liquid, Value-added product,
Antioxidant

Reference:
1. Runzeng Liu; Shanjun Song; Yongfeng Lin; Ting Ruan; Guibin Jiang. Occurrence ofSynthetic Phenolic Antioxidants and Major Metabolites in Municipal Sewage Sludge in
China. Environ. Sci. Technol. 2015, 49, 2073-2080
１８０

2. Stanislaw Kowalski; Marcin Lukasiewicz; Aleksandra Duda-Chodak; Gabriela Zeic. 5Hydroxymethyl-2-Furfural (HMF) – Heat-Induced Formation, Occurrence in Food andBiotransformation – a Review. Pol. J. Food Nutr. Sci. 2013, 63, 207-225
Introduction to the author:
Professor Dr. Sunyoung Bae
Department of Chemistry, Seoul Women‟s University, Seoul, Korea
Tel: +82-2-9705652; Fax: +82-2-9705972.
E-mail: sbae@swu.ac.kr

Biography of the authors.
Miss Soomi Park earns a Bachelor of Science degree in Chemistry at Seoul Women‟s
University in 2015. She currently studies for a master‟s degree in analytical chemistry at
Seoul women‟s university. She has presented her research at Korean Society of Soil and
Groundwater Environment.
Dr. Sunyoung Bae has obtained Ph.D. from the University of Massachusetts-Lowell in
2002. She had been working at the interdisciplinary research center at the University ofNorth Carolina-Charlotte. In 2008, she was appointed as an assistant professor in the
Department of Chemistry at North Carolina A&T State University. Since 2010, she has
been affiliated with Seoul Women‟s Universityas an assistant professor. Her research
approach is to characterize physicochemical properties and interactions in micro-scaleusing analytical instruments that determines macro-phenomena. Currently, she has been
served as an associate editor for Korean Society of Soil and Groundwater Environmentand on the editorial board for the Korean Society of Analytical Sciences.

１８１

PT.25
Analysis of Polycyclic Aromatic Hydrocarbons in Water by Polyaniline
Coated In-needle Microextraction
Yura Hwang, Dong-Sun Lee, Sunyoung Bae*
Department of Chemistry, Seoul Women‟s University*sbae@swu.ac.kr
Abstract:
Polycyclic aromatic hydrocarbons (PAHs) can exist various forms with two or more fusedrings. They are formed naturally or by human beings and mostly generated by the
incomplete burning of coal, oil or organic substances. The concerns for PAHs are theirhazardous characters being potential possibility of causing tumors and reproductive
problems. PAHs are widely present in soil, surface water, ground water, drinking waterand industrial products. To analyze PAHs in aqueous solution, they need to be extracted byvarious techniques including liquid-liquid extraction (LLE) and solid-phasemicroextraction (SPME). LLE method has some limits such as using a large amount oforganic solvents and requiring labor-intensive work. In our study, PAHs in aqueous
solution were determined based on head space in-needle microextraction (HS-INME)
followed by gas chromatography-flame ionization detector (GC-FID). The INME
adsorbent was polyaniline (PANI) that was electrochemically synthesized in our lab.
Among various PAHs, our target compounds were naphthalene, acenaphthylene,
acenaphthene, fluorene, anthracene and fluoranthene. The parameters such as adsorbent
length, adsorbent thickness, extraction temperature, extraction time and desorption time
were optimized. The lengths of PANI layer were varied at 0.5 cm, 1.0 cm, 1.5 cm, and 2.0
cm. CV cycles that might control the adsorbent thickness were varied at 10 cycles, 30
cycles, and 50 cycles. Extraction temperature and time were evaluated at 25ºC, 40ºC, 60ºC
and 10 min, 30 min, 60 min, 90 min, respectively. In addition, desorption time was
evaluated by varying 15 s, 30 s, 60 s, 90 s, and 120 s. In conclusion, HS-INME with PANI
as an adsorbent for PAH analysis can be efficiently performed to shorten analysis time anddevelop the solventless extraction method.
Keywords: Polycyclic aromatic hydrocarbons, head space in-needle microextraction,polyaniline, GC-FID

１８２

Reference:
1. Bagheri, H.; Mir, A.; Babanezhad, E. An electropolymerized aniline-based fiber coating
for solid phase microextraction of phenols from water. Anal. Chim. Acta 2005, 532, 89-95.
2. Li, X.; Zhong, M.; Xu, S.; Sun, C. Determination of phthalates in water samples using
polyaniline-based solid-phase microextraction coupled with gas chromatography. Journalof Chromatography A 2006, 1135, 101-108.
Introduction to the author:
Dr. Sunyoung Bae
Department of Chemistry, Seoul Women's University, South Korea
Tel: +82-2-970-5652; Fax: +82-2-970-5972
E-mail: sbae@swu.ac.kr
Biography of the authors.
Miss Yura Hwang earns a Bachelor of Science degree in Chemistry at Seoul Women‟s
University in 2014. She currently studies for a master‟s degree in analytical chemistry at
Seoul women‟s university. She has presented her research at various conferences including
Korean Chemical Society, The Korean Society of Analytical Sciences, and Korean Societyof Soil and Groundwater Environment.
Dr. Sunyoung Bae has obtained Ph.D. from the University of Massachusetts-Lowell in
2002. She had been working at the interdisciplinary research center at the University ofNorth Carolina-Charlotte. In 2008, she was appointed as an assistant professor in the
Department of Chemistry at North Carolina A&T State University. Since 2010, she has
been working at Seoul Women‟s University. Her research approach is to characterize
physicochemical properties and interactions in micro-scale using analytical instrumentsthat determines macro-phenomena. Currently, she has been served as an associate editor
for Korean Society of Soil and Groundwater Environment and on the editorial board for
the Korean Society of Analytical Sciences.

１８３

PT.26
TNF-α-induced insulin resistance mechanism study based on cell lysisfree quantum dot multicolor cellular imaging-based
Jieun Ki and Joon Myong Song*
College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
TNF-α, an inflammatory cytokine, plays an important role in insulin resistance as
observed in cases of obesity and chronic inflammation. TNF-α is known to inhibit manycellular components involved in the insulin signaling cascade. Insulin receptor substrate(IRS)-1 is one of the major targets in TNF-α-induced insulin resistance and serine
phosphorylation of IRS-1 enables the inhibition of insulin signaling. The mechanism of
TNF-α-induced insulin resistance has been thoroughly investigated based on Western blot.
Intracellular protein kinase crosstalk is commonly encountered in inflammation-associatedinsulin resistance. This crosstalk among the signaling molecules obscures the precise roleof kinases in insulin resistance. Thus, a cell lysis-free quantum dot (QDot) multicolor
cellular imaging was developed to identify the biochemical role of multiple kinases (p38,
JNK, IKKβ, IRS1ser, IRS1tyr, GSK3β, and FOXO1) in inflammation-associated insulin
resistance pathway with a single assay in a single run. QDot-antibody conjugates wereused as nanoprobes to simultaneously monitor the activation/deactivation of the sevenintracellular kinases in HepG2 cells. The effects of the test compounds on the suppressionof TNF-α-induced insulin resistance were validated through kinase monitoring. Aspirin,
indomethacin, cinnamic acid, and amygdalin were tested and the regulatory functions ofmultiple kinases were monitored simultaneously at the cellular level. The developed
cellular imaging assay provides a unique platform to understand the inflammation andinsulin resistance signaling pathways in type II diabetes mellitus and how they regulate
each other. Amygdalin and cinnamic acid were found to inhibit serine phosphorylation of
IRS-1 through targeting JNK serine kinase and enhance insulin sensitivity.
Keywords: Multicolor cellular imaging, Insulin resistance, Quantum dot, Inflammation

１８４

PT.27
Real time high-content cellular monitoring of the cytotoxic action of
cationic polymer carriers polyethylenimine and poly-L-lysine on HepG2
cells
Gee Woo Nam and Joon Myong Song*
College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
Polyethylenimine (PEI) and poly-L-lysine (PLL) are cationic polymers that are commonlyused as non-viral gene/drug delivery vehicles. The two polymers are highly cytotoxic, but
the molecular mechanism regarding their cytotoxicity is poorly understood. Therefore, thetoxicity pathway triggered by PEI and PLL were investigated through a high-content
cellular imaging technique. PEI induced apoptosis via an intrinsic pathway, whereas PLL
showed cytotoxicity through both intrinsic and extrinsic caspase cascades. The apoptotic
activities against HepG2 cells of both PEI and PLL were dependent on the polymericmolecular weight. The structure of the PEI was also found to affect the polymer‟s ability
to cause apoptosis. The branched PEI exhibited greater cytotoxicity than that of the linear
PEI. This observation was verified through Annexin V-FITC/PI assay and real-time highcontent monitoring of cytosolic calcium, mitochondrial membrane disruption, andcaspase-3 activation.

１８５

PT.28Multifunctional Composite of an Antibacterial Silver and a Potent
Wound Healing Polypeptide as a Bimodal Wound Treatment
Baskaran Purushothaman and Joon Myong Song*
College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
The Hst-1-silver (II) chlorhexidine (Hst-1-[Ag(II)CHX]), a multifunctional composite
containing antibacterial bi-valent silver polydiguanide and a potent wound healing
polypeptide, histatin-1, for the treatment of infected wounds. The silver (II) chlorhexidine
was prepared by the oxidation of Ag (I) and followed by the complexation of theoxidized metal with chlorhexidine (CHX), whereas the metal complex conjugate of thesolid phase-synthesized histatin polypeptide (Hst-1), Hst-1-[Ag(II)CHX], was realized bymixing the starting materials in aqueous solution. The structural change for Hst-1 binding
with silver complex was confirmed by circular dichroism spectroscopy. The cell-spreading
assay was performed by using the Hst-1 and synthesized Hst-1-[Ag (II) CHX] complex on
3T3-L1 preadipocytes. The silver complex and its Hst-1 conjugates antibacterial activity
was tested against several gram +ve and gram –ve bacteria, including Methicillin-resistant
Staphylococcus aureus (MRSA) and Methicillin-resistant coagulase negative
staphylococcus (MRCNS) by a broth micro dilution method. The experimental results
revealed that the polypeptide and silver (II) polydiguanide complex retained theirindividual wound healing and antimicrobial activity even in their conjugate. The conjugate
of higher-valent antibacterial [Ag(II)CHX] with a potent wound healing polypeptide (Hst1) showed promise as a new multifunctional therapeutic wherein the killing and healing
functions of the constituent materials are preserved together for the development of newgeneration wound-care agents.

１８６

PT.29Concurrent monitoring of CD31, CD34, CD45 and CD146 endothelial
progenitor cell markers for acute myocardial Infarction
Hee Ju and Joon Myong Song*
College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
The endothelial progenitor cells (EPCs) in blood of acute myocardial infarction (AMI)
patient have been observed in many previous studies. The number of circulating EPC
increases in the blood of patients at the beginning of the AMI. EPC is originated frombone marrow. It executes vessel regeneration. There are many markers used for detecting
EPC. Four of these markers, CD31, CD34, CD45, and CD146, were concurrently detected
at the single cell level for the identification of EPC in the present preliminary study. The
CD45 negative cell sorting was performed to peripheral blood mononuclear cells (PBMCs)
acquired from four AMI patients with a magnetic bead sorter, since, EPCs expressed
CD45 negative or dim. The resultant PBMC eluents were treated with quantum-antibody
conjugates for the probing four different markers of EPCs and then applied to a highcontent single cell imaging cytometer using acousto-optical tunable filter (AOTF). The useof quantum dot, with narrow emission wavelength range and AOTF enabling cellular
image at a particular single wavelength, is very advantageous for accurate high-content
AMI diagnosis based on simultaneous monitoring of many markers. In this work, a highcontent cellular diagnostic assay using quantum dot-antibody probing material and AOTF
is newly suggested to accurately identify EPCs in peripheral blood acquired from CVD
patients. The number of EPC increased as compared with control in three of four AMI
patients. In this approach, two EPC subtypes were found, CD31(+), CD34(+), CD45(/dim), CD146(-) as early outgrowth EPCs and CD31(+), CD34(+), CD45(-/dim),
CD146(+) as late outgrowth EPCs. Patient 1 had CD31(+), CD34(+), CD45(-/dim),
CD146(+) cells whose percentage was 4.21% of cells. Patient 2 had 2.38% of CD31(+),
CD34(+), CD45(-/dim), CD146(-) cells and patient 3 had 4.28% of CD31(+), CD34(+),
CD45(-/dim), CD146(+) cells. This system was proved to be very appropriate for the EPC
monitoring based on its potential to detect large number of molecular markers
concurrently. In addition it was verified that this system provided little spectralinterference and led to more accurate EPC diagnosis.
Keywords: Acute myocardial infarction, Circulating endothelial progenitor cells,
Quantum dot, High content single cell cytometer, Acousto-optical tunable filter (AOTF)
１８７

PT.30Optimization of Chlorophenols Detection in Water Samples using Stirbar Sorptive Extraction Coupled with Thermal Desorption-GC-MS
Mina Lee, Yun S. KIM *Water Quality Reserch Centre, K-water Institute, K-water, Korea
Abstract: The rapid, accurate, and simple analytical method for the determination of trace
amounts of chlorinated phenols, such as chlorophenol, 2,4-dichlorophenol (2,4-DCP),
2,4,6-trichlorophenol (2,4,6-TrCP), 2,3,4,6-tetrachlorophenol (2,3,4,6-TeCP) andpentachlorophenol (PCP), in water using stir bar sorptive extraction (SBSE)-thermal
desorption unit(TDU)–gas chromatography–mass spectrometry (GC–MS) with in-situ
derivatization are described. The optimization of SBSE conditions such as, extraction time,
affection of salt and stirring speed are investigated. The detection limits and quantification
limits of the chlorophenols in water are ranged 0.1~0.6 and 0.4~1.8 ng/mL, respectively.
The average recoveries and precision of the chlorophenols in all the samples are ranged
98~109%, 2.0~6.6%, respectively. This level of detection observed based on hazard
quotient a remarkable improvement over the conventional method. Moreover, thesufficient sensitivity of detection limits and quantitative limits for chlorophenols in water
was estimated based on risk assessment in this work.
Keywords: Chlorophenols; Water; SBSE; Thermal desorption unit (TDU)–GC/MS
Reference:
1. Migaku Kawaguchi, Yumiko Ishii, Norihiro Sakui, Noriya Okanouchi, Rie Ito, Koichi
Saito, Hiroyuki Nakazaw, Stir bar sorptive extraction with in situ derivatization andthermal desorption–gas chromatography–mass spectrometry for determination ofchlorophenols in water and body fluid samples, 2005, Analytica Chimica Acta 533, 2005,
57–65
Introduction to the author:
Researcher Ms. Mina Lee
Water Quality Reserch Centre, K-water Institute, K-water, Korea
Tel: +82-42-629-2047; Fax: +82-42-629-2099.

１８８

Principal Researcher Dr. Yun S. KIM
Water Quality Reserch Centre, K-water Institute, K-water, Korea
Tel: +82-42-629-2042; Fax: +82-42-629-2099.
E-mail: yunskim@kwater.or.kr
Biography of the authors.
Yun. S. Kim is a principal researcher in the water quality research centre of K-water
institute, Daejeon, Korea. He obtained Ph.D (Bioresource Sciences; Environmental
Analysis Chemistry) from Nihon University, Japan. He is interested in the development ofanalysis methods for emerging organic compounds in environmental samples and theirrisk assessments.

１８９

PT.31
Validation of Analytical Method for Propylene Glycol and Ethylene
Glycol in Foods
Ho Soo Lim, Ju Young Hwang, EunA Choi, Gun young Lee and Tae Seok Kang
Food Additives and Packaging Division, Ministry of Food and Drug Safety,
Cheongju-si, chungcheongbuk-do, Korea
E-mail: hslim70@korea.kr
Abstract
A simple method was developed for the simultaneous determination of propylene glycol(PG) and ethylene glycol (EG) in foods using gas chromatography with flame ionization
detector (GC-FID). PG (authorized additive used as humectant and emulsifier) and EG(unauthorized additive having a sweet taste) in foods were extracted with water:acetone(30:70), followed by the treatment of Carrez solutions and centrifugation. The resulting
solution was filtered using 0.45 μm membrane syringe filter and injected into GC. The
separation of PG and EG was carried out on HP-INNOWAX capillary column (30 m 
0.25 mm, 0.25 mm). The calibration curves of PG and EG were linear in the range of 1.0 to
100 μg/ml, with good correlation coefficients (r2 >0.999). The recoveries ranged from 90%
to 98% for PG, from 90% to 104% for EG at spiking levels of 10, 50, and 100 mg/kg in
three samples (dumpling, coffee, candy and chocolate). The limit of detections (LOD) andlimit of quantifications (LOQ) for PG and EG were 0.4 μg/ml and 1.3 μg/ml, 0.3 μg/ml
and 1.0 μg/ml, respectively. For four food matrix studied, expanded uncertainties (U)expressed as percent were below 20% for PG and EG. Uncertainty associated with foodmatrix contributed mostly to the expanded uncertainty. This study is the first report for
direct simultaneous determination of PG and EG in foods by GC-FID.

１９０

PT.32
Dielectric Barrier Discharge Molecular Emission Spectrometer as Gas
Chromatographic Detector for Amines
Chenghui Li a, Xue Jianga, Xiandeng Houa,b*a. Department of Chemistry, Sichuan University, Chengdu, 610064, Chinab. Analytical & Testing Center, Sichuan University, Chengdu, 610064, China
Corresponding author email: houxd@scu.edu.cn
ABSTRACT: Nano-SiO2 was immobilized on the inner wall of a dielectric barrierdischarge (DBD) tube, and it was coupled to a conventional gas chromatographer toinvestigate its performance as a molecular emission spectrometric detector for thedetermination of five volatile aliphatic amines. A charge coupled device (CCD) wasapplied to observe the nano-SiO2-enhanced molecular emission spectra. The characteristic
molecular emission bands of volatile aliphatic amines at 326.5 nm, 336.0 nm and 388.3
nm can be clearly resolved from the background emission spectra of carrier gas argon. The
emission band of CN at 388.3 nm was used for quantitative detection of volatile aliphatic
amines due to its high sensitivity. Nanomaterial catalysts including TiO2, MnO2, SiO2 andZnO were tested to enhance the emission signal of amines, and SiO2 shows the best
performance. The factors that influence the emission signal, such as discharge voltage,
inner electrode length and carrier gas flow rate, were investigated in detail. The analytical
performance of this method was evaluated by separation and detection of the mixture offive volatile aliphatic amines. Under the optimal experimental conditions, the limits ofdetection were found to be 4.4, 2.5, 2.2, 1.8 and 2.4 μg for dimethylamine, trimethylamine,
n-butylamine, cyclohexylamine and ethylenediamine, respectively. This GC detector is not
only sensitive but also fast in response to volatile aliphatic amines with good stability.
Trimethylamine in a carp fish sample was monitored with storage time by the proposed
method.
KEY WORDS: dielectric barrier discharge; molecular emission spectrometry; amine; GC;
SiO2

１９１

REFERENCES
Jiang, X.; Li, C. H.; Long, Z.; Hou, X. D. Anal. Methods 2015, 7, 400-404.
Li, C. H.; Jiang, X.; Hou, X. D. Microchem J. 2015, 119, 108-113.

１９２

PT.33
Aptamer-bound magnetic beads and antibiotics functionalized
fluorescent gold nanoclusters based dual recognition strategy for
detection of bacteria
Dan Cheng, Mengqun Yu, Gan Li, Zheguo Zhu, Yang Song, Erqun Song*Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of
Education, College of Pharmaceutical Sciences, Southwest University, Chongqing,
400715, People‟s Republic of China. Fax: +862368251225; Tel: +862368251225. E-mail:eqsong@swu.edu.cn
Abstract: Infectious diseases and food poisoning caused by pathogenic bacteria such as
Staphylococcus aureus (SA) have been bringing to serious public health concerns1, 2,
which makes the specific, sensitive and rapid detection of bacterial is essential andimportant. The common used strategies for SA including traditional microbiologicalmethod, polymerase chain reaction (PCR)-based method and antibody-based
immunoassay method are limited due to the disadvantages of time-consuming, not suitablefor quantification, false-positive, expensive respectively3-5. Thus, it is essential to develop
an accurate, simple, rapid and cost-effective strategy for SA assay. Besides antibodies,
nucleic acid aptamer, antibiotics are also important recognition molecules for bacteria,
which have been employed for developing detection strategy of SA6,7.
This study presents a strategy by combining the aptamer and antibiotic-based dual
recognition units with magnetic enrichment and fluorescent detection together to achieve
specific and sensitive assay of SA in authentic specimen (Figure 1). Aptamer-boundmagnetic beads (Apt-MB) prepared based on the interaction between biotinlyated-aptamer
and streptavidin-magnetic beads were employed for specific capture and enriching thetarget SA. And the antibiotic of vancomycin stabilized fluorescent gold nanoclusters(AuNCs@Van) synthesized facilely in one step were used for sensitive quantification of
SA. Using this strategy, the determination limit of SA from complex samples containing
other contaminated bacteria could achieve to the level of ppm. Compared with similar job,
our work not only simplifies the preparation procedure of fluorescent detecting probes of
AuNCs@Van, but also detects the added SA in milk directly with good accuracy. And
moreover, aptamer and Van are cheap and widely available, making the strategy for assay
of SA cost effective. Such aptamer and antibiotics based dual recognition strategy
combined with magnetic enrichment and fluorescent detection of trace bacteria show
１９３

promising potential application in the bacteria-related infectious diseases and foodcontamination.

Figure 1 Illustration of Apt-MB and AuNCs@Van based dual recognition strategy for SA
assay.
Keywords: aptamer, antibiotic, gold nanocluster, bacteria, dual recognition
Reference:
1. T. C. Paulitz, R. R. Belanger, Annu Rev Phytopathol 2001, 39. 103-133.
2. T. Kuda, G. Shibata, H. Takahashi, B. Kimura, Food Microbiol 2015, 46. 234-238.
3. C.W. Kaspar, C. Tartera, Methods Microbiol 1990, 22, 497-530
4. A. Atrazhev, D. P. Manage, A. J. Stickel, H. J. Crabtree, L. M. Pilarski, J. P. Acker,
Anal Chem 2010, 82. 8079-8087.
5. C. Y. Wen, J. Hu, Z. L. Zhang, Z. Q. Tian, G. P. Ou, Y. L. Liao, Y. Li, M. Xie, Z. Y.
Sun, D. W. Pang, Anal Chem 2013, 85, 1223-1230.
6. X. Cao, S. Li, L. Chen, H. Ding, H. Xu, Y. Huang, J. Li, N. Liu, W. Cao, Y. Zhu, B.
Shen, N. Shao, Nucleic Acids Res 2009, 37. 4621-4628
7. P.L. H. Hongwei Gu, Kenneth W. T. Tsang, Ling Wang, and Bing Xu, J Am Chem Soc
2003, 125, 15702-15703.
Introduction to the author:
Associate Professor Dr. Erqun Song
College of Pharmaceutical Sciences, Southwest University, Chongqing, China
Tel: +86-15826022758; Fax: +86-23-68251225.
E-mail: eqsong@swu.edu.cn

１９４

PT.34
Determination of the fatty acid compositions offal meat of sheep
produced in Mongolia using GC and GC-MS
G.Oyun1, D.Narantuya1, E.Anudari1, D. Seo2, J. Ha 2,3*
1

Scientific food research and manufacturing “SAMO” Institute, Ulaanbaatar, Mongolia
Food Analysis Center, Korea Food Research Institute3
Korea University of Science and Technology
2

Abstract: The fatty acid compositions of offal meat produced in Mongolian sheep (Ovis
Aries) was analyzed using GC and GC-MS. The present study showed the result that fatty
acid composition in mucous offal meat was higher than the internal organs. Among theinternal organs, liver had most high value of fatty acid. Saturated and unsaturated fatty
acid ratio varied differently depending on the type of animals, such as cow was 1.2: 1, andsheep 1: 1 while the horse was 1: 1.6. Thereby, fatty acid composition of offal include thepalmitic acid C16 and stearic acid C18 from saturated fatty acid branch, oleic acid C18:1
of monoene, linoleic acid C18:2 of polyene from unsaturated fatty acid branch, and otheracids such as С12, С14, С20, С22, С14:1, С16:1, С20:1, С18:3, С20:2, С20:3, С20:4
were found in small quantities. These results show the significant proof of similarity withthe carcass meat of grazing livestock. Thus, we can consider that Mongolian grazing
livestock meat has great beneficiary effect in human health regarding to the fact that theindication of eicosapentaenoic and docosahexaenoic acids were expressed in the analysis
of dried offal meat, while it is not expressed in indoor farming livestock meat. Likewise,“essential fatty acids” which has positive effect in human health make out the 60-80% of
total fatty acid composition in offal meat.
Keywords: saturated, unsaturated, polyenoic, monoenoic fatty acid, sheep, cow, horse,dried sample
Reference:
1. J Am Oil Chem Soc (2010) 87:621–626.
2. International Union of Pure, Applied Chemistry (IUPAC) (1992).
3. Standard methods for the analysis of oils, fats and derivatives. Method 2.403. Blackwell,
Oxford.
4. Bureau of Indian Standards (BIS) (1976) Methods of sampling and test for oils and fats,
１９５

IS: 548 (Part-II; Purity tests).
5. Shukla AK, Singh RP (2005) Detection of sesame oil in other oils and fats. J Oleo Sci
54:543–544.
Introduction to the author:
Professor Dr. Jaeho HA
Food Analysis Center, Korea Food Research Institute, 463-746, Korea
Tel: +82-31-780-9217; Fax: +82-31-780-9280.
E-mail: jhkfri@gmail.com
Biography of the authors.
Dr Jaeho Ha completed his doctorate in food chemistry with the practical combination ofmass spectrometry and gas chromatography at the Korea University in 1992. He joined the
Food Analysis Center at Korea Food Research Institute in 1984, where he involved
himself in the analysis of food components, including micotoxin, pesticides, nutrients,
fatty acid and aromas. Dr. Ha served his vice-presidency of Korean Society of Analytical
Science 2000 – present. He is currently the vice president of Korea Food ResearchInstitute and a leader of food analysis division of Korea Society of Food Science andTechnology. Concurrently, Dr. Ha is applying GCxGC-TOFMS technology coupled withstir bar sorptive extraction and dynamic headspace sampling techniques for the analysis offlavors and trans fatty acids in foods. His research and activity is focused on
chromatographic separation, flavor chemistry, food analysis. Recently he has carried out
the ODA project for the capacity building of agro-food safety system in Mongolia.

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