Inverted Microscopy Platform

50 µm
300 μm x 300 μm wild-type mouse brain section taggedwith DAPI (405 nm), Alexa 488 anti-S100B, Alexa 555
anti-Neurofilament, and Alexa 633 anti-GFAP.

®

A confocal image of cell layers in a mouse retina.
(Sample courtesy of Dr. Robert Fariss, Biological Imaging
Core, National Institutes of Health, Bethesda, MD.)

Inverted Microscopes

Thorlabs’ Modular Inverted Microscopy Platform provides a turnkey solution for
widefield, confocal, and multiphoton imaging. These powerful and versatile
microscopes can accommodate a range of applications such as fluorescence,in vivo, ex vivo, 3D, high-resolution, high-speed, and live tissue imaging. We
offer configurations that support widefield, brightfield, phase, confocal, and/ormultiphoton imaging.

Overview of Veneto® Microscopes
Key Featuresu S upports Widefield, Brightfield, Darkfield,Phase, Confocal, and/or MultiphotonImaging
u U
 nique Reflected Light Illumination Turret for

Easy Access to Filtersu B
 uilt-In Motorized Focusing Module Allowing

for Users to Image Multiple Z-Stacks Easilyu T rans-Illumination Module Tilts to Allow Large

Samples Underneathu L ight Path Selector Allows Users to Switch

Between Up to 3 Light Paths
u T horImage®LS Compatible

Inverted microscopes are powerful and versatile
research tools that can accommodate a range ofapplications, such as fluorescence, in vivo, ex vivo,
3D, high-resolution, high-speed (video-rate), andlive tissue imaging. Our Veneto platform is designed
to meet the needs of labs working in cell biology
and life science applications that requires anStitched confocal image of a complete Drosophila fly head, using a40X objective.

inverted microscopy platform with multiple imaging
modalities, accessory ports, and easily accessible
light paths.
Our Inverted Microscope Platform provides thefoundation for a confocal and/or multiphotonsystem. A multiphoton configuration of theVeneto is shown here, including the Chrolis™
light source, controllers, and a Quantalux®
sCMOS Camera.

Two Microscope Systems in One
The Veneto® microscope is a fully enclosed turnkey system. Nevertheless, the engineers behind this microscope did not want toleave users without the option to implement solutions to their unique experimental challenges. Therefore, Veneto microscopes
are endlessly configurable. DIY optical paths and sample mounting solutions can be easily added to your pre-configured
microscope.

Turnkey System
The Veneto microscope is a fully
enclosed microscope that comes
pre-configured with all the necessary

The enclosed Veneto microscope
provides laser shielding andappropriate covering for dust andlight. The cover is easily removable
for any customizations a user may
want to make.

components required to meet theexact needs of an experiment.
Thorlabs designs and manufactures
the components to ensure seamless
functionality and to enable a high levelof support from Thorlabs’ applicationengineers.
Veneto microscopes are driven by
our ThorImage®LS software, for which
we offer lifetime support. This software
supports multiple imaging techniques
and offers seamless modality changes
when switching between light paths.

DIY System
This system includes many DIY options, including a breadboard on the bottom and sides of the case. This allows users to createcustom optical paths which can be directly incorporated into the Veneto.

The Veneto includes a breadboard
onto which a custom optical pathcan be affixed.

Veneto® Microscope Tour
Two-Photon & Widefield Configuration
Below is an example configuration which includes the Tiberius® fs Ti:sapphire tunable laser, a Pockels cell, and a motorizedvariable attenuator, which together enable two-photon microscopy.

Top TierThis tier includes two major
subsystems--the built-in
focusing module, and the nondescanned detection module
for multiphoton systems.

Bottom Tier
There are many options for thistier, including attaching yourown custom optical path tothe optical breadboard. Otheroptions include preconfigured
modules with resonant-galvogalvo scanners, galvo-resonant
scanners, or a galvo-galvo
scanner.

Featuresu H igh-Speed Imaging via Galvo-Resonant or Resonant-Galvo-Galvo Scanners
u P ockels Cell and Motorized Variable Attenuator for Remote Laser Adjustmentu T wo-Channel Non-Descanned Detector with GaAsP PMTsu T
 iberius® fs Ti:Sapphire Tunable Laser

Trans-Illumination ModuleTiberius® fs Ti:Sapphire
Tunable Laser
Trinoculars with Camera PortCamera Port forLeft-Side Path
Pockels Celland MotorizedVariableAttenuator

Chrolis™ 6-Wavelength
High-Power LED Source

Veneto® Microscope Tour
Confocal & Widefield Configuration
This example configuration includes a four-channel confocal fiber-coupled laser, four PMT detection channels, and a motorizedpinhole wheel.

Middle TierThis tier features the reflected
light illumination moduleand is optimized for evenfluorescence illumination froma liquid light guide or LED
source.

Featuresu H
 igh-Speed Galvo-Resonant or Galvo-Galvo Scanningu M otorized Pinhole Wheel with 16 Sizes from Ø25 µm to Ø2 mmu F our Detection Channels for GaAsP or Multialkali PMTsu F
 our-Channel Confocal Fiber-Coupled Laser

MLS203-1 High-Speed
Scanning Stage
Objective Nosepiece
Trinoculars

Fiber-Coupled LightSource Controller

Reflected Light
Illumination Turret
BBD302 BrushlessServo Controller
High-PowerLED Driver

Highlights and Modules
Built-In Motorized Focusing Moduleu W hole Module Moves using Stepper Motor with 15 mm Travel

The built-in motorizedfocusing module translates
the entire top tier (outlined inred). The pathway includes
the objective nosepieceand the non-descanned
detection module.

Range and 50 nm Resolution
u O ptional Piezo Motor with 100 µm Travel Range and 50 nmResolution
The Veneto® microscope platform
features an innovative built-in
motorized focusing module. This
module translates the entire five-objective
nosepiece, which means a Z-stack can beeasily taken with different objectives by simply
turning the nosepiece to a new objective.

Z

10X Super Apochromatic Objectiveu Ideal for Two-Photon Microscopy
u Infinity-Corrected Dry Objective with Super Apochromatic Axial Color Correctionu 0 .50 NA, 7.77 mm Working Distance, 400 to 1300 nm Transmissionu M
 32 x 0.75 Threading

Objective Compatibilityu F ive-Objective Nosepiece with M25 x 0.75 Portsu S
 ingle M32 x 0.75 Objective Port

The Veneto microscope is often configured with anobjective nosepiece that can accommodate up to fiveM25 x 0.75 objectives. By removing thestandard five-objective nosepiece,
M32 x 0.75 threading is madeaccessible which accepts anobjective with a larger backaperture, such as the TL10X-2P
10X Super Achromatic Objective.

10X Super
Apochromatic
ObjectiveTL10X-2P
10X Super Apochromatic
Microscope ObjectiveLight Path Selector
The light path selector on the Veneto® microscope
platform directs source light on the bottom tier. Users canchoose to enable up to three light paths that can be easily
switched between. There are five light paths that users
can choose from depending on their experimentation
requirements. This selector is motorized and can bedriven via controller or PC, allowing automated switching
between different imaging modalities.

Five Light Path Options for
the Bottom Tieru P rimary Imaging Path using the Rear Port

The Veneto
microscope’s light pathselector showing thethree prism mirrors used
to direct light.

u T rinocular Imaging Path using the Front Portu L eft Side Port with SM2 (2.035"-40) Threads and

60 mm Cage Tapsu R
 ight Side Port with SM2 (2.035"-40) Threads and

60 mm Cage Tapsu S
 M2-Threaded Port Underneath the Light Path

Base Plate

The left and right ports can be easily
adapted to a cage system or camera
to add additional functionality. Thiscan be done in the initial setupof the microscope or later.

High-Speed Scanners
Veneto microscopes can be configured with one or two coregistered scan paths to propagate, condition, and direct aninput laser beam. We offer any combination of galvo-resonant
scanners, galvo-galvo scanners, and resonant-galvo-galvoscanners.
Galvo-Resonant Scanners for High-Speed Imaging
Thorlabs has 8 kHz and 12 kHz galvo-resonant scanners. Our
8 kHz scanners utilize the entire field of view and offer amaximum frame rate of 400 fps, while our 12 kHz scanners
provide an increased frame rate of 600 fps.

Galvo-Galvo Scanners for User-Defined ROI Shapes
Galvo-galvo scanners support user-drawn scangeometries (lines, polylines, squares, and rectangles) andalso support custom photoactivation patterns (circles,ellipses, polygons, and points). They offer consistentpixel dwell times for better signal integration and image
uniformity.

512 x 32

Frames per Second

600
500
512 x 32

400

Line300

0

Square/Rectangle

Circle/Ellipse

Thorlabs offers 8 kHz and 12 kHz resonant-galvo-galvo (RGG)

200
100

Polyline

Resonant-Galvo-Galvo Scanners for Multimodal Scanningscanners. This multimodal scanner provides features of both
512 x 512
8 kHz Scanner

512 x 51212 kHz Scanner

the galvo-resonant and galvo-galvo scanners in a single
scan head.

Highlights and Modules
Trans-Illumination Module20° Tilt

The Veneto® trans-illumination module steers light through the condenser into thetransmitted light optical path. This unit mounts on top of the microscope and is easily
removable should you want to only use the inverted paths. The module tilts back 20°
to allow for clearance when mounting samples. This module is designed for use in
the visible and NIR range (400 – 700 nm) and is ideal for brightfield
and oblique illumination.

Mounted LEDs
Each uncollimated,
mounted LED consistsof a single LED
mounted to a heatsink. This cold white
LED has a broadspectrum.

MCWHLP2
Cold White LED

ThorImage®LS Compatibility

The ThorImageLS software manages the activitiesu L ifetime Supportof Thorlabs’ microscopes and supplementalu Image Acquisition Synced with Hardware Inputs and Timing Eventsequipment to acquire and analyze data sets. Thisu L ive Image Correction and ROI Analysis

software has a range of features and capabilities,u A utomated Image Capture with Scriptsu M ulti-User Settings Save for Shared Workstations

including holographic optogenetics, deep
physiological scans, large-area tiling, high-speedimaging, and multi-modal acquisitions.

The ThorImage® LS software workspace streamlines the image acquisition and analysis process with a user-inuitive, feature-rich workflow.
Panel layouts are completely customizable for different users or imaging modalities. All software features are easily accessible, providing acomplete, self-contained software package without compromising ease of use.

Reflected Light Illumination Module
The reflected light illumination module is included
with all Veneto® microscopes. Designed for evenfluorescence illumination from a liquid light guideor LED source, we commonly configure thiswith Thorlabs’ Solis® High-Power LEDs or Chrolis™
6-Wavelength High-Power LED source.

CHROLIS-C1
6-Wavelength HighPower LED Source

SOLIS-1D
High-Power LED forMicroscopy

The reflected light illumination module conditions a light source for use with thefilter turret.

Six-Position Filter Turretu A ccepts 32 mm x 44 mm Dichroic Filters and Ø32 mmExcitation and Emission Filtersu E asily Removable for Filter Changesu O ptional Motorization Which Can be Controlled via

Controller or PC

Veneto is designed with a unique filter turret for easy accessto the dichroic filters. Once the turret has been removed fromthe microscope, the cover can be easily removed, and thefilters are held in using a small spring to ensure the mounting
does not produce any mechanical stress on the filter. Thisturret can be fully motorized and controlled via controller orthrough software.

The reflected light illumination
module contains Veneto’s
unique six-position filter turret.

Highlights and Modules
High-Speed XY Scanning Stageu U ltra-Fast XY Scanning Up to 250 mm/su H
 igh Repeatability: 0.25 µmu M inimum Achievable Incremental Movement: 100 nmu T ravel Range of 110 mm x 75 mm

The Veneto® microscope platform supports the MLS203-1
Fast XY Scanning Stage, which has a maximum velocity of
250 mm/s and a travel range of 110 mm x 75 mm. There
are many different types of slide holders and accessory
plates for this scanning stage including petri dish holders,slide holders, and multiwell plates. Our team can alsocollaborate with you on a DIY sample holding solution.

The MLS stage has many stage holders and accessory plates that areeasily swappable.

Sample Holders for MLS203-1 Stages

MLS203P1
Multiwell Plate Adapter, Shown with
Multiwell Plate Installed

MLS203P2
Petri Dish/Slide Holder, Shown withPetri Dish Installed

MLS203P10
Multi-Slide Holder,
Shown with Slides Installed

Scientific Camerasu C
 MOS and sCMOS Cameras Availableu C amera Resolutions from 1.3 to 12.3 Megapixels

CS2100M-USB
2.1 Megapixel MonochromesCMOS Camerau F ull-Sensor Frame Rate from 15.1 to 165.5 fps
u C MOS Sensors for Monochrome, Color, NIR-Enhanced,or Polarization-Sensitive Versionsu H
 ousing Options Include Compact, Passively Cooled;

Low-Profile, Passively Cooled; or Hermetically Sealed
TE-Cooled Housings
LP126CU12.3 Megapixel Color CMOS
Camera, Low-ProfileControllersu Z -Stage Focus Controlleru F luorescence Turret Filter Selectoru P
 rimary Reflector Controlu L
 ight Path Selector

These controllers are exclusive to the Veneto microscopes. The
controller pictured to the left is the Z-stage focus controller. The
other controller is used to select one of the light paths, switch theprimary reflector in or out, and, if motorized, switch the filter on
the reflected light fluorescence turret. These features can also
be controlled through our ThorImage®LS software.

CC505MU
5.0 Megapixel Monochrome
CMOS Camera, TE-Cooled

Veneto® Microscope Specifications
Infinity Corrected

Optical System

20

Optical Field Number

450 - 1100 nm, 680 - 1300 nm, or 800 - 1800 nm

Scan Path Wavelength Range

Resonant-Galvo-Galvo Scanner, Galvo-Resonant Scanners, orGalvo-Galvo Scanners; Single or Dual Scan Paths

Scan Paths

Laser Scanning

8 kHz Resonant-Galvo-Galvo or GalvoResonant Scan Speed

2 fps at 4096 x 4096 Pixels
30 fps at 512 x 512 Pixels
400 fps at 512 x 32 Pixels

12 kHz Resonant-Galvo-Galvo or GalvoResonant Scan Speed

4.4 fps at 2048 x 2048 Pixels
45 fps at 512 x 512 Pixels
600 fps at 512 x 32 Pixels

Field of View

20 mm Diagonal Square (Max) at the Intermediate Image
Plane [12 mm Diagonal Square (Max) for 12 kHz Scanner]

Scan Zoom

1X to 16X (Continuously Variable)
Up to 2048 x 2048 Pixels (Bi-Directional)[Up to 1168 x 1168 Pixels for 12 kHz Scanners]Up to 4096 x 4096 Pixels (Unidirectional)[Up to 2336 x 2336 Pixels for 12 kHz Scanners)

Scan Resolution

Trinoculars with 10X Eyepieces and Camera Port for 1X
Camera Tube with External C-Mount Threads

Observation Tube

Light Source

Brightfield: Thorlabs Mounted LED
Widefield: LED or Liquid Light Guide through Chrolis™
6-Wavelength High-Power LED Source or Solis® High-Power LED
Laser Scanning: Tiberius® Ti:Sapphire Femtosecond Laseror Single Mode Fiber-Coupled Laser Source with up to 4
Excitation ChannelsCondenser

LWD 0.52 NA
3.5 mm Translation with Trans-Illumination Module110 mm x 75 mm (4.3" x 2.95") Travel Range
250 mm/s Max Velocity

Motorized XY Scanning Stage
Objective Turret

Five Manual Positions with M25 x 0.75

Focusing Unit

15 mm of Travel
Stepper Motor: 15 mm Travel Range, 50 nm Resolution
Piezo: 100 µm Travel Range, 50 nm Resolution

Reflected Light Illumination Turret

Six Motorized Positions for 32 mm x 44 mm Dichroics andØ32 mm Excitation and Emission Filters
Stage Joystick and Microscope Control Pad

Control Units

Confocal Laser Source Options
Excitation Wavelengths# ofLasers

UV2-334

Included Emission FiltersRedEmission Filters (Center Wavelength/Bandwidth)

Longpass Dichroic Cutoff
Wavelength(s)488 nm 561 nm-525 nm/45 nm and 600 nm/52 nm

573 nm405 nm 488 nm 561 nm-

440 nm/40 nm, 525 nm/45 nm, and
600 nm/52 nm

495 nm and 573 nm

488 nm 561 nm 642 nm

525 nm/45 nm, 600 nm/52 nm, and607 nm/Longpass

561 nm and 635 nm

405 nm 488 nm 561 nm 642 nm

440 nm/40 nm, 525 nm/45 nm,
600 nm/52 nm, and 647 nm/Longpass

495 nm, 573 nm, and 647 nm

-Blue

Green

Worldwide Support

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