Animal-free science at cellasys
J. Wiest
2018/11/16
Berlin / Germany

Dr.-Ing. Joachim Wiest
wiest@cellasys.com

Willkommen

cellasys facts– Privately owned Germany based SME– Founded 2007 as spin off from Technische Universität München– Provider of instrumentation / services for analysis of living cells– Equipped with laboratories for electronics, electrochemistry, machine
shop and cell culture laboratory according to ISO 9001, ISO 13485 andgood laboratory practice (GLP)– Working at the interface between electronics and living systems– Knowledge in interdisciplinary fields as engineering, cell-biology,informatics, toxicology, …
IntroLabel-free monitoring of living cells

From McConnell H. M. et al.: The Cytosensor Microphysiometer: Biological Applications of Silicon Technology, Science, 1992, 257,
1906-1912

Metabolism of living cells
Cellular respiration

Extracellular acidification
Adhesion / Morphology
Adapted from McConnell H. M. et al.: The Cytosensor Microphysiometer: Biological Applications of Silicon Technology, Science,
1992, 257, 1906-1912

BioChip-D- Extracelluar acidification- Cellular respiration- Temperature- Optical access- Resistant to 70% EthanolBioChip-D microsensors

24 mm- Bio-impedance6xIMOLA-IVD
Parallel, label-free monitoring of cellularvitality via monitoring of extracellularacidification, cellular respiration and bioimpedance.

Weiss, D.; Brischwein, M.; Grothe, H.; Wolf, B.; Wiest, J., "Label-free monitoring of whole cell vitality," in EMBC, 2013 35th Annual
International Conference of the IEEE , vol., no., pp.1607-1610, doi: 10.1109/EMBC.2013.6609823

Vegan lab- Established an animal-product free cell culture lab:Kolar, R., Wiest, J., Feil, M. and Weber, T.: Chemically defined cell culture
practice of L929 fibroblasts, ALTEX Proceedings, 2017, 6, 1, 203, ISSN
2194-0479- Adopted L929 to chemically defined medium
van der Valk, J., Bieback, K., Buta, Ch., Cochrane, B. Dirks, W.G., Fu, J.,
Hickman, J.J., Hohensee, Ch., Kolar, R., Liebsch, M., Pistolla, F., Schulz,M., Thieme, D., Weber, T., Wiest, J., Winkler, S., Gstraunthaler, G.: Fetal
Bovine Serum (FBS): Past – Present – Future, ALTEX, 2018, 35/1, 99118, doi:10.14573/altex.1705101- Developed an FBS free test for cytotoxicity:Wiest, J.: Chemisch definiert - ein zellbasierter Zytotoxizitätsassay ohne
fötales Kälberserum. BIOspektrum, 2017, 23: 61. doi:10.1007/s12268017-0768-6

www.cellasys.com

Cytosensor – Eye Irritation

Effect of seven dilutions of SDS toward L929 fibroblasts(left: extracellular acidification rate; right: impedance).
Eggert, S.; Alexander, F.A.; Wiest, J., "An automated microphysiological assay for toxicity evaluation," in EMBC, 2015, 37th
Annual International Conference of the IEEE , vol., no., pp.2175-2178, doi: 10.1109/EMBC.2015.7318821

28 day repeated dose (Trovafloxacin)

Brischwein M., Wiest J.: Microphysiometry. In: Bioanalytical Reviews, Springer, 2018, https://doi.org/10.1007/11663_2018_2

3D spheroid monitoring

Oxygen monitoring

Alexander, F., Eggert, S., Wiest, J.: A novel lab-on-a-chip platform for spheroid metabolism monitoring, Cytotechnology, 2018, 70/1,375-386, DOI 10.1007/s10616-017-0152-x

Skin-on-a-chip

Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical resistance and extracellular acidification
measurements through an automated air-liquid interface, Genes, 2018, 9/2, 114; doi:10.3390/genes9020114

Dankeschön - Thank you!

Further information:www.cellasys.com

Dankeschön