Product nameContents

Phytoplasma Universal Detection Kit

48 tests

AbstractThis kit is the product to universally detect Candidatus Phytoplasma species by isothermal
amplification reaction using LAMP (Loop-mediated Isothermal Amplification) method. It can betested whether Phytoplasmas exist in host plants with this product. Infection of Phytoplasmas is
judged by the amplification of DNA. This kit is effective for the highly-sensitive diagnosis ofPhytoplasma disease. This kit allows the whole reaction process to proceed at a constanttemperature in an incubator. Thermal cycling machine is not needed for this kit.
Phytoplasmas are plant pathogenic bacteria associated withdevastating damage to over 700 plant species worldwide. They aretransmitted by insect vectors. Infected plants show a wide range ofsymptoms including stunting, yellowing, witches’ broom, phyllody,virescence, proliferation, purple top, and phloem necrosis.

Example of Phytoplasma disease:
Hydrangea phyllody

Product overview

All the necessary contents are included
Other reagents are not necessary
Simple and easy
Extract DNA from host plants easily and start immediately
Clear judgement
Visible fluorescence suggests the result
Low risk of contamination
No electrophoresis is neededHigh reliability of oligonucleotide
Reliable oligonucleotide made in conformity with ISO13485 used as raw materials
Contents

Phytoplasma Extraction Solution
Phytoplasma Detection Solution
Phytoplasma Positive Control
Mineral Oil
Fluorescent Detection Solution
Phytoplasma Enzyme Solution
Test Tube
Instruction Manual

1. Dispense Phytoplasma Extraction Solution 5. Dispense Test Solution into each Test Tube
into each microtube (100.0 µl per tube)(23.0 µl per tube)
Extraction
Solution 100.0 µl

6. Add DNA sample prepared in process 1-3 toeach Test Tube (2.0 µl per tube)
7. Add Mineral Oil (20.0 µl per tube)

2. Cut off calyx or leaf vein of hydrangea and 8. Incubate at 64˚C for 60 minutes (DNAsoak it in the Phytoplasma Extraction
amplification)
Solution
9. Heat at 80˚C for 2 minutes (Enzymeinactivation)
2 x 5 mm
2 x 5 mm

10. Judgement of negative or positive withfluorescence

3. Incubate at 95˚C for 10 minutes (DNAextraction)
4. Prepare Test Solution
Reagents

1 testPhytoplasma Detection Solution

21.0 µl189.0 µl

Fluorescent Detection Solution1.0 µl

9.0 µlPhytoplasma Enzyme Solution

1.0 µl9.0 µl23.0 µl

207.0 µl

Total

8+1 tests*Examples* Prepare 1 test extra solution to prevent deficiency of solution when dispensing.

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Address 2-7-18, Toiya-machi, Toyama 930-0834 Japan
TEL +81-76-451-6548 FAX +81-76-451-6547
URL http://nippongene-analysis.com
E-mail support@nippongene-analysis.com

License:Phytoplasma Universal Detection Kit is developed under
the LAMP technology license from Eiken Chemical Co.,
Ltd. Nippon Gene Co., Ltd. has been granted the license
to development, manufacture and sell the kit for
Phytoplasma identification.

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