CLINICAL STUDY BRIEF

Reduction in Tissue
Processing Turnaround
Time Without theUse of Heat
Qiu Xian Thong
Department of Anatomical Pathology, Ultimed Scientific Sdn Bhd, Malaysia

Natahsa Najwa Binti No Arfuzir
Department of Anatomical Pathology, Ultimed Scientific Sdn Bhd, Malaysia

Muhammad Najib bin Abu Husin

Department of Anatomical Pathology, Epredia, USA
Correspondence: abuhusin.muhammadnajibin@epredia.com

Vijayaletchumi Marimuthi
Laboratory of Histopathology Sunway Medical Center, Malaysia

Tissue processing is the procedure in anatomy pathology to replace the water in tissue with paraffin. This
critical step in the process can preserve the tissue long-term. However, tissue processing is time-consuming
and often requires more than 14 h. Revos innovative features, such as the canted retort with patented
rotational agitation, enable tissues to be processed more rapidly while maintaining molecular quality.
Epredia™ Revos™, an automated tissue processor that was designed for all types of tissue processing.
Revos processed a wide variety of surgical specimens with grossing thicknesses ranging from 2 mm to 8 mm
in this study. The results indicated that Revos optimally processed 2 mm of non-fat-rich tissues and 3 mm of alltissues utilizing protocols ranging from two to five hours, including a 7-hour protocol with surgical specimens
up to 6 mm in thickness, optimally processed with a 7-hour protocol. All tissues processed showed good H&E
and Immunohistochemical stains. The protocol of rapid tissue processing using the Revos automated tissue
processor has reduced turnaround time by approximately 50%.

Revos tissue processor.

Heat is commonly usedto process tissues ata faster rate. However,heating over 35 ºC could
adversely damage theprotein (Horobin et al.,1998), DNA, and RNA oftissues (Srinivasan et al.,2022). Interestingly, unlike
other conventional rapid
tissue processors, which
apply heat to 90 ºC, theRevos automated tissue
processor did not solely
depend on heat to achieve
rapid processing.

AB

DE

Figure 1. H&E staining of tissues that were
processed with 7h protocol. The tissues were (A)lungs, (B) fibroid, (C) lipoma, (D) kidney and (E)colon. All tissues were stained well and shown
good microscopic structures.

CRapid processing

Revos workflow-enhancingtissue processor

The Revos tissue processor’s unique,canted chamber enhances reagent
distribution, reduces tissue processing
time, and allows for high-quality processing results.

The Revos tissue processor’s
design is optimized for bothroutine and rapid processing.

Figure 2. The IHC staining of control
tissues that were routinely used by
the IHC team at Sunway Medical
Centre Laboratory (namely Control)and IHC staining of tissues processed using the Epredia Revos tissueprocessor (namely Revos). The IHC
staining location and intensity were insignificantly different between Control
and Revos. The IHC result indicatedthat the effectiveness of Epredia
Revos automated tissue processor
in preserving the protein structure inpreserving the protein structure andantigenicity of specimens.

Epredia RevosTissue Processor• Quality• Consistency• Ease of use

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