Animal

Plant

Cell

DNA

JCM

Info

植物リソースリスト

植物リソースリスト

Animal

１．リストの見方
書ではこれらのリソースの概要について説明いたします。個別のリソースの詳細情報につきまして

Plant

2010 年春現在、実験植物開発室から提供するリソースは合計 50 万系統を越えております。本
は当室ホームページにてご確認をお願いいたします。

植物培養細胞rpc

各種植物培養細胞

P23
P23

Info

植物遺伝子材料
pda シロイヌナズナ完全長 cDNA クローン(全長塩基配列解析済み)
pdx, pdy, pdz
シロイヌナズナ完全長 cDNA クローン(原則端読み配列のみ)
pdp, pdr
ヒメツリガネゴケ完全長 cDNA クローンpds ポプラ完全長 cDNA クローンpdm キャッサバ完全長 cDNA クローン
pby タバコ BY-2 培養細胞由来 EST クローン（完全長 cDNA 含む）
pdc ハクサイ EST クローン（完全長 cDNA 含む）
pdh Thellungiella halophila の完全長 cDNA クローンpdw Striga hermonthica の完全長 cDNA クローン
rpd 個別の遺伝子クローン、ベクター

JCM

シロイヌナズナ種子
pst シロイヌナズナトランスポゾンタグライン（ホモ/ヘテロ未判別）
psh シロイヌナズナトランスポゾンタグライン（ホモ種子系統）
pss シロイヌナズナアクティベーションタグラインまたは FOX ライン（20 プールのセットの番号）
psp シロイヌナズナアクティベーションタグラインまたは FOX ライン（50 系統を混合したプールの番号）
psa シロイヌナズナアクティベーションタグライン（理研 BRC で樹立した個別の系統の番号）
psm シロイヌナズナアクティベーションタグライン（理研 GSC で樹立した個別の系統の番号）
psx シロイヌナズナ FOX ライン（シロイヌナズナ遺伝子過剰発現系統の種子プールを構成する個別の系統）
psr シロイヌナズナ FOX ライン（イネ遺伝子過剰発現系統の種子プールを構成する個別の系統）
psi 個別のシロイヌナズナ変異体･形質転換体
sja ほか sj で始まる系統
シロイヌナズナ野生株、変異体及び近縁種の系統（SASSC 由来系統含む）

DNA

リソースの付加情報について： カタログではリソース付加情報として寄託者から得た情報と理研BRCで新たに取得した情報を表示しております。例えばシロイヌナズ
ナ完全長cDNAの場合、クローンの塩基配列がシロイヌ
ナズナゲノムの塩基配列と完全一致しない場合もありま
すが、理研BRCではクローンの配列が寄託者の解析した結果と一致することをもって提供可としております。付
加情報については必ずホ−ムペ−ジのカタログ等により確認されてから提供申込みを行ってください。

Cell

リソース番号について： 当室より提供するリソースには固有のリソース番号が付与されております。通常リソー
スには系統名がつけられますが、リソース番号をつける
ことにより、（１）リソースをカテゴリー別に仕分けできる、（２）l（英文字）と１（数字）などまぎらわしい名前を持つリソースを区別することができる、（３）野生株のライン化等、
理研BRCにおいて派生的なリソースを整備する際に対応しやすい、などの利点があります。当室のリソース番
号はリソースのカテゴリーを表すアルファベット３文字（下記表を参照ください）と５桁の数字から成ります。

Animal
Plant
Cell
DNA
JCM
Info

２．シロイヌナズナ種子（１）トランスポゾンタグライン
Resource number: pst00001-pst20808
Developer: Drｓ. Takuya Ito, Takashi Kuromori, Motoaki Seki, Reiko Motohashi, Takashi Hirayama andKazuo Shinozaki (RIKEN)
Total number of lines: 17,672
15,267
Style of distribution:① Approx. 50 seeds of individual line are provided.
② When homozygous seed stock is requested, we ship approx. 500 homozygous seeds withwild-type Nossen seeds for control.
Number of available lines: 15,267 lines
Background ecotype: Nossen (unknown ecotype for Ds12 and 16)
Name of the vector used for transformation: pCGN derivatives
Selection marker: hygromycin
Specific feature:① Flanking sequences of transposon have been characterized.
② Homozygous seed stock is available for over 2,700 lines.
Specific terms and conditions:① When the RECIPIENT has filed a patent application on the invention conceived or made during
the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT
agrees to promptly notify RIKEN Plant Science Center (the owner of the BIOLOGICALRESOURCE) upon filing the application with specifications.
② The recipient can receive up to 50 lines of the BIOLOGICAL RESOURCE in each year.
③ The RECIPIENT agrees to expressly describe that “the BIOLOGICAL RESOURCE (theresource name) was developed by the plant genome project of RIKEN Genomic Sciences
Center, and provided by the RIKEN BRC through the National Bio-Resource Project of the
MEXT, Japan” in Materials and Methods or in the Acknowledgement of any publication
reporting the use thereof. The manuscript(s) listed below should be referred in the publication
on scientific journal. The RECIPIENT also agrees to send a copy of such publication to the
RIKEN BRC. The RIKEN BRC may disclose publicly, copy or otherwise use such publication
to demonstrate the contribution by the RIKEN BRC.
References:① Fedoroff NV. and Smith D. (1993) A versatile system for detecting transposition in Arabidopsis.
Plant J. 3: 273-289.
② Smith D., Yanai Y., Liu, Y-G., Ishiguro S., Okada K., Shibata D., Whittier RF. and Fedoroff NV.
(1996) Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertionalmutagenesis. Plant J. 10: 721-732.
③ Ito T., Motohashi R., Kuromori T., Mizukado S., Sakurai T., Kanahara H., Seki M. andShinozaki K. (2002) Plant Physiol. 129: 1695-1699.
④ Kuromori T., Hirayama T., Kiyosue Y., Takabe H., Mizukado S., Sakurai T., Akiyama K.,
Kamiya A., Ito T. and Shinozaki K. (2004) Plant J. 37: 897-905.
⑤ Ito T., Motohashi R., Kuromori T., Noutoshi Y., Seki M., Kamiya A., Mizukado S., Sakurai T.
and Shinozaki K. (2005) Plant Cell Physiol. 46: 1149-1153.
Important note:① AGI codes are frequently changed. Before you make your order, we recommend you to obtain
detail information including flanking sequence of the line from RARGE (Riken Arabidopsis
Genome Encyclopedia) or T-DNA express.
② The lines marked 'In preparation' are currently unavailable. Do not include those lines in yourorder. We will make an announcement at the time they are ready for distribution.
③ The transposon-tagged lines are categorized to genetically modified organisms. For orderingsuch materials, the applicants must obtain all of the permission required by any kind of
regulation or law before ordering. Also, it is recipient's responsibility to perform customs
procedure for importation of biological materials. Please inform us when you need quarantine

P24
P24

（２）アクティベーションタグライン
Resource number: pss00001-pss00009, developed by RIKEN BRC (The pss00009 contains 17 seed pools.
Others contain 20 seed pools.); pss00101-00128, developed by RIKEN GSC (The pss00103 contains
16 seed pools. Others contain 20 seed pools.).
Developers: Drs. Motoaki Seki, Miki Nakazawa, Minami Matsui, Masatomo Kobayashi and Kazuo
Shinozaki (RIKEN)
Total number of line: approx. 70,000
Style of distribution: Provided as a seed pool set (equivalent with 1,000 lines). Each seed pool set
contains 20 seed pools, and one seed pool contains approx. 400 seeds from 50 lines; that means 8
seeds per line in average. (Please see “Structure of activation tagged-line” shown below.) Seeds
for individual lines (psa and psm) that constitute seed pool (psp) are available for the users who
obtained desired mutant in the seed pool.

P25
P25

Info

通常挿入位置を確認するためには Tail-PCR などの

JCM

トランスポゾンの挿入位置の確認について：DNA

世界各地で採取されたシロイヌナズナ野生系統を分類するときにエコタイプ（環境型）という言葉が使われます。タグラインがどのエコタイプより作成されたかは重要な意味を持ちます。
アクティベーションタグラインでは、ゲノム塩基配列が解析された Columbia 株に T-DNA を導入して作成されたため、挿入位置の解析には公開されているゲノムの塩基配列が適用でき、ほとんどの場合は一義的に挿入位置を決定することができると考えられます。
一方 Nossen 株にトランスポゾンを導入しているトランス
ポゾンタグラインの場合、解析した端読み配列とColumbia 株のゲノム塩基配列との間に塩基置換などの多型が存在することがあり、トランスポゾンの挿入位置を100％の確率では決定できない場合がしばしば見られます。このためカタログデータベースでは、挿入位置の候
補を２つまで表示しておりますのでご確認をお願いいた
します。

Cell

方法により隣接配列の解析を行います。トランスポゾン
タグラインはほとんどの場合、ゲノム上に 1 箇所の挿入がおこるため解析は比較的容易です。当室から提供するトランスポゾンタグラインについては開発者の理研
GSC により決定された挿入位置情報がカタログに掲載され、また遺伝子名を用いてオンライン検索ができるようになっております。なお理研 BRC では提供前にデータベースに記載の位置にトランスポゾンの挿入があること
を PCR により確認しています。
一方 T-DNA タグラインではゲノム上の複数の位置に
T-DNA の挿入がおこりうるため、挿入位置を決定する
際には注意が必要です。タンデムリピートや T-DNA の一部が挿入の際に欠失するなど挿入様式が複雑で解析が困難となる可能性にもご注意ください。
なおトランスポゾンタグライン、T-DNA タグライン両者
とも挿入位置と異なる場所に変異が存在することが少なからずあります。このため表現型の解析においては同一遺伝子座に変異のある複数のラインを使用するか、ないしは候補遺伝子の過剰発現株／発現抑制株を用いて表現型を確認されることをお勧めします。

Plant

バックグラウンドのエコタイプについて：

Animalcertification to import Arabidopsis seeds. Without providing such information, RIKEN BRC
cannot guarantee any loss or damage of the materials that is caused during customs procedure.
④ Although the background ecotype of this resource has been reported as No-0 (Nossen), we
found polymorphisms between the No-0 line from SASSC and the parental lines provided by
Dr. Fedoroff. We recommend users to use the parental lines available from ABRC as control
plants in your experiments.
Information
requiredbybyCartagena
CartagenaProtocol
ProtocolononBiosafety
Biosafetypart):(in(inpart):
Information required
This BIOLOGICAL MATERIAL corresponds to Living Modified Organisms destined forcontained use. (Arabidopsis thaliana)
Requirements for the safe handling, storage, transport and use:
① This BIOLOGICAL MATERIAL is the seeds of Arabidopsis thaliana.
② This BIOLOGICAL MATERIAL harbors NPTII, HPT and GUS genes (from E.coli), ALS
gene (from A. thaliana), Ds element (from Zea mays), promoter of CaMV 35S RNA and
CaMV 19S RNA genes (from califlower mosaic virus), and LB, RB and NOS-terminator
sequences of T-DNA (Agrobacterium tumefaciens).
③ This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it
for "Intentional introduction into the environment".
④ This BIOLOGICAL MATERIAL and its progeny should be inactivated by autoclave or
equivalent procedure after use.

Animal
Plant
Cell
DNA
JCM
Info

Number of available pool sets: 37
33 seed pool sets (equivalent with 36
32, 650 lines)
Background ecotype: Columbia
Name of the vector used for transformation: pPCVICEn4HPT
Selection marker: hygromycin
Specific feature: The 4 x CMV 35S enhancer in T-DNA may promote gene expression near T-DNA
insertion site to produce dominant phenotype.
Specific conditions for distribution:(For pss00001-00009) If the RECIPIENT claims any kind of intellectual property obtained from the use of
the BIOLOGICAL RESOURCE, the RECIPIENT is required to notice RIKEN BRC after application.
(For pss00101-00128) When the RECIPIENT has filed a patent application on the invention conceived or
made during the course of research studies directly using the BIOLOGICAL RESOURCE, theRECIPIENT agrees to promptly notify RIKEN Plant Science Center (the owner of the BIOLOGICALRESOURCE) upon filing the application with specifications.
Reference:① Nakazawa M., Ichikawa T., Ishikawa A., Kobayashi H., Tsuhara Y., Kawashima M., Suzuki K.,
Muto S. and Matsui M. (2003) Plant Journal 34(5): 741-750.
Information required by Cartagena Protocol on Biosafety (in part):
This BIOLOGICAL MATERIAL corresponds to Living Modified Organisms destined forcontained use. (Arabidopsis thaliana)
Requirements for the safe handling, storage, transport and use:
① This BIOLOGICAL MATERIAL is the seeds of Arabidopsis thaliana.
② This BIOLOGICAL MATERIAL harbor HPT and ampicillin-resistant genes (fromE.coli), promoter of CaMV 35S RNA gene (from califlower mosaic virus), and LB, RB
and NOS-promoter sequences of T-DNA (Agrobacterium tumefaciens).
③ This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it
for "Intentional introduction into the environment".
④ This BIOLOGICAL MATERIAL and its progeny should be inactivated by autoclave or
equivalent procedure after use.

（３）FOX ライン（シロイヌナズナ遺伝子過剰発現系統）
11
Resource number: pss10001-10010
Developer: Drs. Minami Matsui, Miki Nakazawa and Takanari Ichikawa (RIKEN)
Total number of line: approx. 20,000 (expected)
Style of distribution: Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines= 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds
for individual lines (psx) that constitute specific seed pool (psp) are available for the users who
obtained desired mutant in the seed pool.
10,796lines)
lines)
Number of lines available: 11seed
5 seed pool set (equivalent with 5,000
Background ecotype: Columbia
Name of the vector used for transformation: pBIG2113SF (pBI121 derivative)
Selection marker: hygromycin
Specific feature: Provided as seed pools for screening. Arabidopsis full-length cDNA (RAFL) was
introduced with 35S promoter to produce dominant phenotype.
Specific conditions for distribution:① When the RECIPIENT has filed a patent application on the invention conceived or made during
the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT
agrees to promptly notify RIKEN Plant Science Center upon filing the application withspecifications.
② When the RECIPIENT publishes his/her research, acknowledge RIKEN PSC and referdesignated manuscripts shown below.
Reference:① Ichikawa T, Nakazawa M, Kawashima M, Iizumi H, Kuroda H, Kondou Y, Tuhara Y, Suzuki K,
Ishikawa A, Seki M, Fujita M, Motohashi R, Nagata N, Takagi T, Shinozaki K and Matsui M(2006) Plant J. 48:974-985.

P26
P26

P27

Info

P27

JCM

（５）野生系統・近縁種及び変異体系統
仙台シロイヌナズナ種子保存センター（SASSC）から寄託された AIS 由来の世界標準となるリソースを中心に提供しています。世界各地の環境に適応した野性のシロイヌナズナは様々な遺伝的変異を持ち、今後の研究に有望なリソースです。そこで当室では遺伝型、表現型に関する情報をカタログに追加するべく準備を進めております。ぜひご活用をお願いいたします。

DNA

Information required by Cartagena Protocol on Biosafety (in part):
This BIOLOGICAL MATERIAL corresponds to Living Modified Organisms destined forcontained use. (Arabidopsis thaliana)
Requirements for the safe handling, storage, transport and use:
① This BIOLOGICAL MATERIAL is seed of Arabidopsis thaliana.
② This BIOLOGICAL MATERIAL harbor HPT gene (from E.coli), promoter of CaMV 35S
RNA gene (from cauliflower mosaic virus), cDNA from Oryza sativa, and LB, RB andNOS-terminator sequences of T-DNA (Agrobacterium tumefaciens).
③ This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it for"Intentional introduction into the environment".

Cell

Resource number: pss11001, pss11002
Developer: Dr. Minami Matsui (RIKEN PSC)
Total number of line: approx. 20,000 (expected)
Style of distribution: Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines= 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds
for individual lines (psx) that constitute specific seed pool (psp) are available for the users who
obtained desired mutant in the seed pool.
Number of pool sets available: 2 seed pool set (equivalent with 2,000 lines)
Background ecotype: Columbia
Vector for transformation with Agrobacterium: pBIG2113SF(pBI121 derivative)
Selection marker: hygromycin
Specific feature: Provided as seed pools for screening. Rice full-length cDNA was introduced with 35S
promoter to produce dominant phenotype.
Specific conditions for distribution:(1) The RECIPIENT agrees to expressly describe that “the BIOLOGICAL RESOURCE (the resourcename) was developed by the RIKEN PSC, NIAS and RIBS through The Special Coordination
Fund for Promoting Science and Technology, and provided by the RIKEN BRC through the
National Bio-Resource Project of the MEXT, Japan” in Materials and Methods or in the
Acknowledgement of any publication reporting the use thereof. The RECIPIENT also agrees tosend a copy of such publication to the RIKEN BRC. The RIKEN BRC, RIKEN PSC, NIAS andRIBS may disclose publicly, copy or otherwise use such publication to demonstrate thecontribution by the RIKEN BRC.

Plant

（４）FOX ライン（イネ遺伝子過剰発現系統）

AnimalInformation
requiredbybyCartagena
CartagenaProtocol
ProtocolononBiosafety
Biosafetypart):
Information required(in(inpart):
This BIOLOGICAL MATERIAL corresponds to Living Modified Organisms destined forcontained use. (Arabidopsis thaliana)
Requirements for the safe handling, storage, transport and use:
① This BIOLOGICAL MATERIAL is seed of Arabidopsis thaliana.
② This BIOLOGICAL MATERIAL harbor HPT gene (from E.coli), promoter of CaMV 35S
RNA gene (from cauliflower mosaic virus), cDNA from Arabidopsis thaliana, and LB, RB
and NOS-terminator sequences of T-DNA (Agrobacterium tumefaciens).
③ This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it for"Intentional introduction into the environment".

Animal
Plant
Cell
DNA
JCM
Info

目次
シロイヌナズナ野生系統の分類について：在する場合があります。理研 BRC では SSLP 解析技術を適用して保有する野生系統の遺伝型情報を収集するとともに、主だった系統より同質系統の確立を進めています。

野生系統を収集する際には一箇所の生息地から複数の個体を採取することが普通でこれに同一の名称（エコタイプ）をつけて増殖維持するため、個体間に多型が存

a. AIS wild type populations
Resource number: SJA00100-SJA26400(SJAxxx00 means bulk seeds and
SJAxxx01-xxx05 means seeds fromindividual plant.)
Number of available lines: 264
Origin of resource: AIS
Style of distribution: Approx. 50 seeds of individualline are provided.
NOTE: Some of the lines need quite a long period
until flowering.

f. AIS trisomic lines
Resource number: SJT00100-SJT02400
Number of available lines: 23
Origin of resource: AIS
g. AIS other species
Resource number: SJS00100-SJS01200
Number of available lines: 12
Origin of resource: AIS
h. Sendai wildtype populations
Resource number: SJW10100-SJW14400
Number of available lines: 45
Origin of resource: SASSC (Dr. Goto)b. AIS form mutants
Resource number: SJF00100-SAJ20500
Number of available lines: 206
Origin of resource: AIS

i. Sendai mutants
Resource number: SJG00100-SJG14000
Number of available lines: 113
Origin of resource: SASSC (Dr. Goto)c. AIS virescent (leaf color) mutant
Resource number: SJV00100-SJV41100
Number of available lines: 321
Origin of resource: AIS

j. Mutants from M. Koornneef’s collection
Resource number: SJK00100-SJK02400
Number of available lines: 24
Origin of resource: SASSC (Dr. Koornneef)

d. AIS metabolic mutants
Resource number: SJE00000-SAJ01300
Number of available lines: 13
Origin of resource: AIS

k. Sendai other species
Resource number: SJO00100-SJO03000
Number of available lines: 28
Origin of resource: SASSC (Dr. Goto)e. AIS gene marker lines
Resource number: SJM00100-SJM10500
Number of available lines: 106
Origin of resource: AIS

P28
P28

psi00007

DFL2OX

psi00008

DFL2AS

Activation tagging
Over expression
Anti-sense

psi00009

DFL-2GUS

GUS at hypocotyl

psi00010

pCPC::CPC:GFP

psi00011

stop1

psi00012

stop1-KO

psi00013

stop1-comp

psi00014

gid1 Aabbcc

psi00015
psi00016
psi00017

gid1 bc (AAbbcc)
gid1 ac (aaBBcc)
gid1 ab (aabbCC)
gid1 a-1(aaBBCC)
gid1 a-2(aaBBCC)
gid1 b-1(AAbbCC)
gid1 c-1(AABBcc)srd2-1
rid3
rid5
rpd1-1
rpd1-2
rgd3-1

GFP at epidermal cell
Senstive to acid/Al
stressSenstive to acid/Al
stressSenstive to acid/Al
stress (complement
line)
Severe dwarf (ifhomozygous)
Mostly normal
Dwarf
Low fertility

Crossing (double KO)
Crossing (double KO)
Crossing (double KO)

None

Ds insertion

None

T-DNA insertionNone

Ds insertion

None

T-DNA insertion

Temperature sensitive
Temperature sensitive
Temperature sensitive
Temperature sensitive
Temperature sensitive
Temperature sensitive

EMS
EMS
EMS
EMS
EMS
EMS

psi00018
psi00019
psi00020
psi00021
psi00022
psi00023
psi00024
psi00025
psi00026
psi00027

P29
P29

EMS
T-DNA insertion
Compliment line of stop1
Crossig (triple KO)

14581632
14581632
14581632
12403712
17535918
17535918
17535918
17521411
17521411
17521411
17521411
17521411
17521411
17521411
17521411
20965997
19054368
14522871
16407439
16407439
19054368

Info

Transformation with
GUS
Transformation with GFP

14871309

JCM

lsh1-D

Method for establishment
Activation tagging
Activation tagging
Activation tagging
Activation tagging

DNA

psi00006

Phynotype
Dwarf
Dwarf
Dwarf
Dwarf
Reduced elongation of
hypocotyl
Reduced elongation of
hypocotyl
Reduced elongation of
hypocotyl

CellLine number
iso-1D
iso-2D
iso-4Dydk1-D

Reference(PubMedID)
12787254
12787254
12787254
14756757

Plant

Resource
number
psi00001
psi00002
psi00004
psi00005

Animal（６）個別の変異体・形質転換体
論文で発表された変異体、形質転換体を提供します。現在提供可能な系統は以下のとおりです。詳細についてはホームページでご確認ください。

Animal
Plant
Cell
DNA
JCM
Info

３．植物培養細胞2012 年 1 月現在、38 株を提供しています。培養条件の詳細はホームページでご確認ください。
（１）シロイヌナズナ T87 細胞モデル実験植物シロイヌナズナの代表的な培養細胞です。葉緑体が発達するため緑色をしており、通常明所で培養します。通常より低い温度（22℃）で培養することに注意が必要です。
リソ−ス番号： RPC00008
由来植物名： Arabidopsis thaliana L. ecotype Columbia
培養状態： 懸濁培養
培養条件： 明所（連続白色光、40-100 ・E/s/m2）、22℃、旋回培養（120 rpm）
培地：JPL 培地
寄託者： Micheke Axelos (INRA)
提供条件： 一般 MTA に同じ
参考文献：(1) Axelos M., Curie C., Mazzolini L., Bardet C. and Lescure B. (1992) Plant Physiol. Biochem.
30(1): 123-128 (2) Callard D., Axelos M. and Mazzolini L. (1996) Plant Physiol. 112: 705-715（２）タバコ BY-2 細胞
増殖が速く同調培養が可能な世界標準の培養細胞株として広く知られています。使用例は細胞増殖機構の研究、タンパクの細胞内局在の解析、物質生産技術の開発等多岐にわたります。
リソ−ス番号： RPC00001
由来植物名： Nicotiana tabacum L. cv. Bright Yellow
培養状態： 懸濁培養
培養条件： 暗所、27℃、旋回培養（130 rpm）
培地： Linsmaier and Skoog 改変培地
寄託者： Toshiyuki Nagata (Tokyo Univ.)
提供条件： 一般 MTA に同じ
参考文献： Nagata T., Nemoto Y. and Hasegawa S. (1992) International Review of Cytology 132: 1-30

（３）イネ OS-1 細胞
同調培養可能なイネの培養細胞系として樹立されました。静置培養と懸濁培養の双方で保存しております。提供依頼の際にはどちらかを指定してください。
リソ−ス番号： RPC00020
由来植物名： Oryza sativa L.
培養状態： 懸濁培養、静置培養
培養条件： 暗所、27℃、懸濁培養の場合には旋回培養（110 rpm）
寄託者： Yuko Ohashi (NIAS)
提供条件： 一般 MTA に同じ
参考文献： 中曽根正一、南栄一、今井剛、秋山典昭、大橋祐子 (1993) イネの培養細胞における同調培養系の開発および細胞周期の進行に伴うヒストンH3とPCNA遺伝子の発現 農業生物資源研究所報告 8: 1-10（４）遺伝子組換え植物培養細胞
東京大学の馳澤博士より寄託を受けたタバコ培養細胞株、GV7(rpc00039), GF11(rpc00040), GT16(rpc00041) を提供します。それぞれ液胞膜、アクチン繊維、微小管が GFP により可視化されている細胞株です。ご利用の際には以下の点にご注意ください。
• 本リソースは遺伝子組換え技術を適用して樹立された植物培養細胞ですが、自然条件で個体へと再分化しないた
め、そのまま実験に使用される場合は法律で定める遺伝子組換え生物に該当しません。ただし何らかの技術を用いて本リソースを含有する組織または個体を作成する場合には法律の対象となりますのでご注意ください。
• 本リソースは GFP 遺伝子を組み込んでいます。利用条件については巻頭の説明もご覧ください。

P30
P30

Animal（５）その他の植物培養細胞
細胞株名

由来植物名

培養状態

備考

RPC00002

Kurodagosun

ニンジン

懸濁培養

RPC00003

VR

ブドウ

静置培養

RPC00004

VW

ブドウ

静置培養

RPC00005

PAR

ヨウシュウヤマゴボウ

静置培養

RPC00006

PAP

ヨウシュウヤマゴボウ

静置培養

RPC00007

PAW

ヨウシュウヤマゴボウ

静置培養

RPC00009

T-13

タバコ

懸濁培養

RPC00011

Cba-1

スイカ

静置培養

RPC00012

Sly-1

トマト

静置培養

RPC00014

CRA

ニチニチソウ

懸濁培養

RPC00015

V208

ニチニチソウ

懸濁培養

RPC00016

Spi-WTホウレンソウ

懸濁培養樹立者の承諾書が必要

RPC00017

Spi-I-1

ホウレンソウ

懸濁培養樹立者の承諾書が必要

RPC00018

Spi-12F

ホウレンソウ

懸濁培養樹立者の承諾書が必要

RPC00021

Vma-1

ツルニチニチソウ

静置培養

RPC00022

A.per

アスパラガス

静置培養

RPC00023

A.pas

アスパラガス

静置培養

RPC00024

A.plo

アスパラガス

静置培養

RPC00025

PSB

ゴマ

静置培養

RPC00026

PSW

ゴマ

静置培養

RPC00027

PSG

ゴマ

静置培養

RPC00028

LcyD6

ヘチマ

静置培養

RPC00029

LcyD7

ヘチマ

静置培養

RPC00030

ZE3

ヒャクニチソウ

静置培養

RPC00031

Oc

イネ

懸濁培養

RPC00032

Lj

ミヤコグサ

懸濁培養

RPC00033

LjA

ミヤコグサ

静置培養

RPC00034

Ljm

ミヤコグサ

静置培養

RPC00035

Xan-1

タバコ

静置培養

RPC00036

BY-2H

タバコ

静置培養

RPC00037

M18-1

ムラサキ

静置培養

RPC00038

OM

ムラサキ

静置培養

Plant

リソース番号

Cell
JCM

寄託者の承諾書が必要

P31

Info

以上の株の詳細はホームページに掲載している植物培養細胞カタログを参照してください。

P31

DNA

寄託者の承諾書が必要

Animal
Plant
Cell
DNA
JCM
Info

４．植物遺伝子
我が国で作成されたモデル植物の完全長 cDNA を中核とした整備を進めています。シロイヌナ
ズナに加えヒメツリガネゴケ（コケのモデル）、ポプラ（樹木のモデル）、キャッサバ（バイオ燃料のモ
Thellungiella halophilaデル）、タバコ（ナス科のモデル）、ハクサイ（アブラナ科作物のモデル）、Thellungiellahalophila（ストレス研究のモデル）、Striga
cDNA リソ−ス合計 50
Striga hermonthica（寄生植物のモデル）の完全長
hermonthica
万クローン以上を取り揃え、内外の研究者に提供しております。
完全長cDNAの品質について：れている可能性も考えられ、今後の課題です。
(3) 予測された転写領域が存在しない位置や２個の転写領域にまたがるクローン、あるいは１個の転写領域が別々に転写されたクローン
遺伝子予測プログラムの不完全さなどにより差が生じると考えられ、完全長クローンのデータに基づき
アノテーションが修正されてきました。
以上のクローンに生物学的意味があるのかどうかについては意見がわかれるところですが、リソースセンターとしては請求があれば提供できるよう体制を整備することが重要と考え、カタログに配列とともに掲載しています。
そこでクローンの提供申込み時には必ず配列をご確認いただくようお願いいたします。カタログどおりの配列を持つクローンについては提供後のクレームに応じかねますので、よろしくお願いいたします。

完全長 cDNA の作成には数種のプロトコルが確立されており、プロトコルによって差はあるもののいずれの方法
でも完全長率が高いライブラリーを作成することができます。しかし実際にシロイヌナズナの完全長ライブラリーを構成する個々のクローンについて詳しく調べると、ゲノ
ム上に予測されている遺伝子配列と異なる以下のようなクローンを見出すことができます。
(1) 塩基置換があるクローン
Columbia 株の多型によるもの、クローン化過程での変異によるもの等が想定されます。
(2) 転写開始点、エキソン−イントロンの構造が異なるク
ローン
不完全な転写物より cDNA が作成された可能性もありますが、転写段階において遺伝子機能が調節さ（１）シロイヌナズナ完全長 cDNA (RAFL)クローン

This resource has been developed by RIKEN Genomic Sciences Center (GSC). The clones were
deposited after the characterization of their full-length sequence at RIKEN GSC, SSP (Salk/Stanford/PGEC)
consortium and National Institute of Genetics (NIG) partially supported by NSF 2010 Project and National
Bioresource Project. The clones are called as ‘R clone’ in the SSP consortium.
Resource number: pda00001- (with full-length sequence), pdx00001-/pdy00001-/pdz00001- (with
5’-and/or 3’-end sequences)
Developers: Drs. Motoaki Seki and Kazuo Shinozaki (RIKEN).
Total number of clones: 251,382 clones
Style of distribution: Approx. 100 ng of lyophilized plasmid is provided.
Number of available clones: 251,382 clones
Background ecotype: Columbia
Vector: modified pBluescript
Selection marker: ampicillin
Map and restriction site: Shown on our HP.
Specific feature: Full-length sequence and/or 5’-/3’-end sequences have been characterized.
Specific conditions for distribution:① When the RECIPIENT has filed a patent application on the invention conceived or made during
the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT
agrees to promptly notify RIKEN Plant Science Center (the owner of the BIOLOGICALRESOURCE) upon filing the application with specifications.
② The maximum number of the clones that can be ordered from one laboratory or group in one
order is 100.
③ When you publish your research, acknowledge RIKEN GSC and refer designated manuscripts.
References:① Seki, M., Carninci, P., Nishiyama, Y., Hayashizaki, Y. and Shinozaki, K. (1998) Plant Journal
15: 707-720.
② Seki, M., Narusaka, M., Kamiya, A., Ishida, J., Satou, M., Sakurai, T., Nakajima, M., Enju, A.,

P32
P32

Resource number: pds00001Developers: Drs. Tokihiko Nanjo and Kenji Shinohara (FFPRI)
Total number of clones: 23,100
Style of distribution: Approx. 100 ng of lyophilized plasmid is provided.
Number of available clones: 23,100
Organism: Populus nigra var. italica (poplar)
Vector: pME18SFL3 (Gene Bank Accession No. AB009864, TOYOBO, Japan)
Selection marker: ampicillin
Map and restriction site: Shown on the catalogue of TOYOBO.
Specific feature: 5’- and 3’-end sequences have been characterized.
Specific conditions in MTA:
① The RECIPIENT should obtain approval of the DEPOSITOR using the APPROVAL FORM
prior to entering THE AGREEMENT with the RIKEN BRC.

P33
P33

Info

This material has been developed and characterized by National Institute for Basic Biology (FFPRI).

JCM（３）ポプラ完全長 cDNA クローンDNA

Resource number: pdp00001-,
pdp00001- pdr00001Developer: Dr. Mitsuyasu Hasebe (NIBB)
Total number of clones: 149,363
Style of distribution: Approx. 100 ng of lyophilized plasmid is provided.
Number of available clones: 149,363 clones
Organism: Physcomitrella patens (moss)
Vector: modified pBluescript (pFLC-1-E)
Selection marker: ampicillin
Map and restriction site: Please see the figure on our HP.
Specific feature: 5’- and 3’-end sequences have been characterized.
Specific conditions for distribution:① The manuscript listed below should be referred in the publication on scientific journal.
② This material will not be used for research for commercial parties or otherwise forcommercial purposes. For detail please send E-mail to plant@brc.riken.jp.
Reference:① Nishiyama T., Fujita T., Shin-I T., Seki M., Nishide H., Uchiyama I., Kamiya A., Carninci P.,
Hayashizaki Y., Shinozaki K., Kohara Y. and Hasebe M. (2003) Comparative genomics ofPhyscomitrella patens gametophytic transcriptome and Arabidopsis thaliana: implication forland plant evolution. PNAS 100 (13): 8007-8012.
NOTE: Detailed information on the individual clone is available from the following website:
http://moss.nibb.ac.jp

Cell

This resource has been developed by the collaboration of National Institute for Basic Biology (NIBB)
and RIKE GSC, and the sequence was characterized at National Institute of Genetics (NIG).

Plant（２）ヒメツリガネゴケ完全長 cDNA クローン

AnimalAkiyama, K., Oono, Y., Muramatsu, M., Hayashizaki, Y., Kawai, J., Carninci, P., Itoh, M., Ishii,Y., Arakawa, T., Shibata, K., Shinagawa, A. and Shinozaki, K. (2002) Science 296: 141-145.
NOTE:① Detailed information including full-length sequence of the individual RAFL clone is available
from the following websites:
http://rarge.gsc.riken.jp/
http://signal.salk.edu/cgi-bin/tdnaexpress② Please examine the sequence of clones that you have interest by yourself.
③ Clone numbers pda99001-99400 correspond to RIKEN Arabidopsis Transcription Factor(RARTF) ORF clones. For detail description of this resource, please visit our website,
http://www.brc.riken.jp/lab/epd/Eng/catalog/orf.shtml.

Animal
Plant
Cell
DNA
JCM

② The BIOLOGICAL MATERIAL should not be used for any commercial purpose.
③ The manuscript shown below should be referred in the publication on scientific journal.
④ The RECIPIENT can receive up to 10 clones in one order and up to 100 clones in one year as
the BIOLOGICAL RESOURCE.
⑤ The RECIPIENT should not distribute or transfer the BIOLOGICAL RESOURCE to any
other laboratory or organization.
⑥ Within 6 months after receiving the BIOLOGICAL RESOURCE, the RECIPIENT must
sequence the entire length of the insert(s), deposit the sequence(s) into
EMBL/GeneBank/DDBJ database, and report the accession number(s) to the depositor via
the RIKEN BRC. The RIKEN BRC can use them to improve the catalogue in RIKEN BRC.
Reference:① Nanjo T., Futamura N., Nishiguchi M., Igasaki T., Shinozaki K. and Shinohara K.,
Characterization of full-length enriched expressed sequence tags of stress-treated poplar
leaves. Plant Cell Physiol. 45 (12): 1738-1748 (2004).
NOTE: More information is available from:http://www.ffpri.affrc.go.jp/labs/kouho/Press-release/2004/cdna041220.html

Info（４）キャッサバ完全長 cDNA クローンThis resource has been developed and characterized by RIKEN PSC under the collaboration with CIAT.
Resource number: pdm00001Developer: Drs. Motoaki Seki and Kazuo Shinozaki (RIKEN PSC)
Total number of Clones: 19,968
Style of distribution: Approx. 100 ng of lyophilized plasmid is provided.
Number of clones available: 19,968 clones
Organism: Manihot esculenta Crantz.
Vector: pFLCIII
Selection marker: ampicillin
Map and restriction site: Carninci et al. (2001) Genomics 77(1-2): 79-90.
Specific feature: 5'- and 3' -sequences have been characterized.
Specific conditions for distribution:① The RECIPIENT can use the BIOLOGICAL RESOURCE for research only.
② The RECIPIENT agrees to refer the RIKEN PSC and CIAT as developers and the RIKEN
BRC as a provider of the BIOLOGICAL RESOURCE in any publication reporting use of it.
③ Following manuscript should be referred in the publication on scientific journal. Sakurai, T.,
Plata, G., Rodriguez-Zapata, F., Seki, M., Salcedo, A., Toyoda, A., Ishiwata, A., Tohme, J.,
Sakaki, Y., Shinozaki, K. and Ishitani, M. Sequencing analysis of 20,000 full-length cDNA
clones from cassava reveals lineage specific expansions in gene families related to stressresponse. BMC Plant Biology, 2007, 7:66.
④ The RECIPIENT agrees not to use the BIOLOGICAL RESOURCE for constructing
microarray or macroarray without the agreement from the RIKEN PSC.
⑤ The RECIPIENT should not distribute or transfer the BIOLOGICAL RESOURCE to any
other laboratory or organization.
⑥ The RECIPIENT can receive up to 10 clones in one order and up to 100 clones in one year as
the BIOLOGICAL RESOURCE.
Reference:① Sakurai, T., Plata, G., Rodriguez-Zapata, F., Seki, M., Salcedo, A., Toyoda, A., Ishiwata, A.,
Tohme, J., Sakaki, Y., Shinozaki, K. and Ishitani, M. Sequencing analysis of 20,000
full-length cDNA clones from cassava reveals lineage specific expansions in gene families
related to stress response. BMC Plant Biology, 2007, 7:66.
NOTE: More information is available from the following website:
http://www.riken.jp/engn/r-world/info/release/press/2007/071206/index.html

P34
P34

P35
P35

Info

Resource number: pdc00001-02166 (EST clone), pdc10001-19903 (full-length cDNA clone)
Developer: Drs. Hiroshi ABE and Yoshihiro NARUSAKA
Total number of Clones: 2,166 (EST clone); 9,903 (full-length cDNA clone)
Style of distribution: Approx. 100 ng of lyophilized plasmid is provided.
Number of clones available: 2,166 (EST clone); 9,903 (full-length cDNA clone)
Organism: Brassica rapa subsp. Pekinensis
Vector: pBluescript II SK(-) (EST clone); pGCAP10 (full-length cDNA clone)
Selection marker: ampicillin
Map and restriction site: Please see the figure in supplier’s catalogue. For pGCAP10, please visit
http://dna.brc.riken.jp/pdf3/vector/AB371573.pdf.
Specific feature: 5'-sequences have been characterized (EST clone); 5’- and 3’-sequences have beencharacterized (full-length cDNA clone)
Specific conditions for distribution: Following manuscripts should be referred in the publication onscientific journal.
(for EST clone)

JCM

This resource has been developed by Drs. Hiroshi ABE, Masatomo KOBAYASHI (RIKEN BRC), and Dr.
Yoshihiro NARUSAKA (Research Institute for Biological Sciences OKAYAMA).

DNA（６）ハクサイ EST クローン（完全長 cDNA 含む）

Cell

Resource number: pby00101Developer: Dr. Ken Matsuoka (RIKEN GSC)
Total number of clones: 22,221
Style of distribution: Approx. 100 ng of lyophilized plasmid is provided.
Number of available clones: 22,221 clones
Organism: Nicotiana tabacum BY-2 cultured cell
Vector: pGEM T-Easy vector (Promega) (pby00101-pby14996); pBluescript II KS+ (Stratagene)(pby21001-35384); pGCAPSP2 (pby40001-)
Selection marker: ampicillin
Map and restriction site: Please see the figure in supplier’s catalogue. For pGCASP2, please send
your mail to plant@brc.riken.jp.
Specific feature: 5’- and 3’-end sequences have been characterized.
Specific conditions for distribution:① These materials will be distributed for basic research of no commercial value.
② When result of research using this resource is planned to claim right or to use for industrial
purpose, researchers should discuss with RIKEN before starting steps.
③ If commercial use of the clone(s) is planned, contact RIKEN to discuss the condition of thecommercial use.
④ Following manuscripts should be referred in the publication on scientific journal reportinguse of it.
References:① Matsuoka, K., Demura, T., Gális, I., Horiguchi, T., Sasaki, M., Tashiro, G. and Fukuda, H.
(2004) A comprehensive gene expression analysis toward the understanding of growth anddifferentiation of tobacco BY-2 cells. Plant Cell Physiol. 45:1280-1289.
② Gális, I., Šimek, P., Narisawa, T., Sasaki, M., Horiguchi, T., Fukuda, H. and Matsuoka, K.
(2006) Tobacco microarray reveals novel MYB transcription factor that selectively activates
genes in phenylpropanoid metabolism. Plant J. 43:573-592.
NOTE: Detailed information on the individual clone is available from following website:
http://mrg.psc.riken.go.jp/strc/index.htm

Plant

This resource has been developed by RIKEN Plant Science Center (PSC). Some of the clones were
established as “full-length cDNA clones”.

Animal（５）タバコ BY-2 培養細胞由来 EST クローン（完全長 cDNA 含む）

Animal
Plant
Cell
DNA
JCM
Info

Narusaka et al. (2006) Comparative analysis of expression profiles of counterpart gene sets
between Brassica rapa and Arabidopsis thaliana during fungal pathogen Colletotrichum
higginsianum
Plant Biotech.analysis
23: 503-508.
Narusaka
et al.infection.
(2006) Comparativeof expression profiles of counterpart gene sets(for full-lengthcDNA
clone) rapa and Arabidopsis thaliana during fungal pathogen Colletotrichum
betweenBrassica
Abe
et al. (2011)
Developmentof full-length
cDNAs from Chinese cabbage (Brassica rapa
higginsianum
infection.
Plant Biotech.
23: 503-508.
subsp.cDNA
pekinensis)(for full-lengthclone)and identification of marker genes for defence response. DNA Res. 18(4):277-289.
Abe et al. (2011) Development of full-length cDNAs from Chinese cabbage (Brassica rapa
subsp. pekinensis) and identification of marker genes for defence response. DNA Res. 18(4):277-289.
（７）Thellungiellahalophila 完全長 cDNA クローンThis resource has been developed and deposited by Drs. Motoaki Seki, Tetsuya Sakurai and Kazuo（７）
Thellungiella
完全長
cDNA
クローンJapan. The 5’- and 3’-end sequence information was
Shinozaki,
RIKEN halophila
Plant Science
Center,Yokohama,

depositedfrom Dr.has
TeruakiTokyo Universityof Agriculture.
Full-length
obtained
underThis resource
beenTaji,developed
and depositedby Drs. Motoaki
Seki,sequences
Tetsuya were
Sakuraiand Kazuo
thesupport RIKEN
from thePlant
National
Bio-Resource
Project of the
MEXT,
Shinozaki,
Science
Center, Yokohama,Japan.
The Japan.
5’- and. 3’-end sequence information was
deposited from Dr. Teruaki Taji, Tokyo University of Agriculture. Full-length sequences were obtained underResource
pdh00001the support
fromnumber:the National
Bio-Resource Project of the MEXT, Japan. .
Developer: Drs. Motoaki Seki, Tetsuya Sakurai and Kazuo Shinozaki (RIKEN PSC)
Total numberof Clones:
xxxxx
Resource
number:pdh00001Style of distribution:
Approx.
ng ofSakurailyophilized
plasmid
is provided.
Developer:
Drs. Motoaki
Seki,100
Tetsuyaand Kazuo
Shinozaki(RIKEN PSC)
Numberof clones
available:
xxxxx clones
Total numberof Clones:
xxxxx
19,429Organism:
Thellungiellahalophila
Style of distribution:
Approx.
100 ng of lyophilized plasmid is provided.
Vector:(excersized
lambdaFLCIII)
NumberpFLCIIIof clones
available:fromxxxxx
clones19,429Selectionmarker:ampicillin
Organism: Thellungiella halophila
Map andpFLCIII
restriction
site: Carninci
et al. (2001) Genomics 77(1-2): 79-90.
Vector:(excersizedfrom lambdaFLCIII)
Specificand 3' -sequences have been characterized. For some clones, full-length sequence
Selectionfeature:marker:5'-ampicillin
available. site: Carninci et al. (2001) Genomics 77(1-2): 79-90.
Map andisrestriction
Specific conditions
feature: 5'-foranddistribution:
3' -sequences have been characterized. For some clones, full-length sequence(1) The RECIPIENT
is available. can use the BIOLOGICAL RESOURCE for research only.
(2) Theconditions
RECIPIENT
to refer the RIKEN PSC as a developer and the RIKEN BRC as aSpecific
for agrees
distribution:
providerofthe
BIOLOGICALRESOURCE RESOURCE
in any publication
reportinguse of it.
(1) The RECIPIENT can use the BIOLOGICAL
for research
only.
(3)
manuscript
shouldreferredin the publication
scientificand
journal.
(2) Following
The RECIPIENT
agreesto berefer
the RIKEN
PSC as a on
developer
the RIKEN BRC as a
Taji,T., Sakurai,T., Mochida, RESOURCE
K., Ishiwata, in
A.,anyKurotani,A., Totoki,Y.,useToyoda,providerof the BIOLOGICAL
publication
reportingof it. A., Sakaki, Y.,
Seki, M., manuscript
Ono, H., should
Sakata,beY.,
Tanaka,S. publicationand Shinozaki,
K. Large-scalecollection and(3) Following
referredin theon scientificjournal.
annotationof full-length
enrichedfrommodel halophyte,
Thellungiellahalophila.
Taji,T., Sakurai,T., Mochida,K.,cDNAs
Ishiwata,
A.,a Kurotani,A., Totoki,Y., Toyoda,A., Sakaki, Y.,(4) Theshouldnot distributeor transfer
BIOLOGICALRESOURCE
to any otherSeki,RECIPIENT
M., Ono, H.,
Sakata,Y., Tanaka,
S. and the
Shinozaki,
K. Large-scalecollection
andlaboratoryororganization.
annotation of full-length enriched cDNAs from a model halophyte, Thellungiella halophila.
(5)
use the BIOLOGICAL
for constructingor(4) The
The RECIPIENTRECIPIENTagrees
shouldnot
nottodistributeor transfer theRESOURCEBIOLOGICALRESOURCEmicroarrayto any othermacroarray
the agreement from the RIKEN PSC.
laboratory orwithout
organization.
(6)
TheRECIPIENTRECIPIENT
can not to use the BIOLOGICAL RESOURCE for constructing microarrayreceive up(5) Theagreesorto
10 clones
in oneorder
andmacroarray
without
the agreement from英文詳細画面のページ（pdw
the RIKEN PSC.
公開 Dec.11）
up
100
clones incan
one
year
the
(6) The
ThetoRECIPIENTRECIPIENT
canreceive up to 10 clones in one order and up to 100 clones inreceiveup
oneasyear
BIOLOGICAL
to the10 BIOLOGICAL
clones in oneRESOURCE. 英文詳細画面のページ（pdw 公開 Dec.11） order andas
RESOURCE.
up to 100 clones in one
year as the(For detail please send e-mail to plant@brc.riken.jp)(Fordetail please send e-mail to plant@brc.riken.jp)
BIOLOGICALRESOURCE.
(For
detail please sende-mailto plant@brc.riken.jp)（８）Striga hermonthica
完全長
cDNA
クローン
This resource has been deposited from Drs. Satoko Yoshida and Ken Shirasu (RIKEN Plant Science（８）
Strigahermonthica 完全長 cDNA クローン
Center(PSC)).
ResourceThis
resourcenumber:has beenpdw00001-35198
deposited from Drs. Satoko Yoshida and Ken Shirasu (RIKEN Plant Science
CenterDeveloper:(PSC)). Drs. Satoko Yoshida and Ken Shirasu (RIKEN PSC)
Resource
number:pdw00001-35198
Total numberof Clones:
37,632Developer:
Drs. Satoko
YoshidaandngKenShirasu (RIKEN
PSC)
Style of distribution:
Approx.
100
of lyophilized
plasmid
is provided.
Total numberof Clones:
37,632
Numberof clones
available:
35,198Organism:
Striga hermonthica
Style of distribution:
Approx. 100 ng of lyophilized plasmid is provided.
Vector:NumberpAL17.3
of clones available: 35,198
Organism: Striga hermonthica
P36
Vector: pAL17.3

P36
P36

Animal
Plant

Selection marker: ampicillin
Map and restriction site: Please visit website http://brc.riken.jp/lab/epd/Eng/catalog/pAL17-3-1.pdf.
Specific feature: 5’- and 3’-sequences have been characterized (full-length cDNA clone)
Specific conditions for distribution: Following manuscript should be referred in the publication onscientific journal.
Yoshida et al. (2010) A full-length enriched cDNA library and expressed sequence tag
analysis of the parasitic weed, Striga hermontica. BMC Plant Biology 10: 55.

Cell（９）その他の遺伝子材料

P37

Info

P37

JCM

Resource number: rpd00105
Resource name: pYLTAC7
Developer: Dr. Daisuke Shibata
Depositor: Mitsui Chemical Co.
Type of resource: vector DNA (TAC vector)
Size: 22.5 kbp
Style of distribution: Approx. 1 micro gram of lyophilized DNA is provided.
Selection marker: kanamycine
Map and restriction site: Please see the figure in the manuscript below.
Accession no.: AB020028
Specific feature: Able to accept and maintain large genomic DNA fragment in both E. coli andAgrobacterium tumefaciens.
Specific conditions for distribution:① The recipient should obtain approval of the depositor using the approval form prior toentering the agreement with the RIKEN BRC.
② The BIOLOGICAL MATERIAL should not be used for any commercial purpose. For detailplease send E-mail to plant@brc.riken.jp.
Reference:① Liu Y-G, Shirano Y., Fukaki H., Yanai Y., Tasaka M., Tabata S. and Shibata D. (1999) PNAS
96 (11): 6535-6540.

DNA

Most of the materials in this category have been deposited to RIKEN Plant Cell Bank. RIKEN BRC
started distribution of the materials from Apr. 2001. The most requested material, pYLTAC7, is explained
below. Information for other materials is on our HP.