Against common belief！
Changing ”Regular water” into ”Ultra water”
Introducing the technology

MARUYAMA ”UFB Ultra Pump”
Introduction【Aquaculture／Agriculture】

Ultra Fine Bubble（UFB）is:

UFB=
Micro sized tiny bubble same size as flu virus（diameter less than1μm）

The bubble is invisible and does not burst

BubbleFine Bubble
Ultra Fine Bubble

bacteria

Ultra Fine Bubble
1/300 of Pine pollen

Micro Bubble

1/10 of bacteria（μm）

Name of the bubble changes accordingly to its size
But it also has different characteristics accordingly to the size
2UFB characteristics changes water performance and function✓ High wash power✓ Activates physiology✓ Destroy virus & bacteria✓ It can contain various gas✓ It penetrate deep into the✓ Proven safety for humans

smallest of spaces～Already at work!～
MedicalLife

Periodontal treatment, body
cavity wash in surgery, andothers

Increased wash power inwash machine and 洗toilet.
Adding flavor to foodAgri/Aqua culture

BeautyIncreased harvest volume
and sweetness in tomato
field. Water quality andmortality rate improved inaquaculture

Used in Showerhead, UFB
contained lotion that havehigh absorption

UFB is Awesome！
But, the problem was the size of UFB machine and its cost

3Ultra Fine Bubble Pump
ＭＡＲＵＹＡＭＡ’s leading technology made it possible
For the machine to be small, low cost, and high performance！
MARUYAMA UFB pump line up

Other company

ProductImagePowerUFB Qty.

Triple 200V

Power

Battery

Battery

Single100VSingle100V

Triple200V

GasolineengineOne pass 0.46mil

UFB
Qty.

2 mil./1ml

2 mil./1ml

2 mil. /1ml

2 mil./1ml

4 mil./1ml

4 mil./1ml

３０min. Circulation 4 mil.

TypeSetOutput２０ℓ/30 min

UFB gen.
MethodOne pass method, generates bubbles at the same time as output

TypeBack Pack

Hand Held

Hand Held

Set/Movable

Set/Movable

Movable(Outdoor)

MPa

２2

22

３

3

Output/min

１．４

1.4

1.4

1.4

２０20

吐出口径

G１／４

G1/4

G1/4

G1/4

G１／２

G1/2

The size small enough to be parts and its low cost
Maruyama pump can generate a few million bubbles in 1cc water in justseconds

4

UFB Case Study in Aquaculture

5Preventing “death” in the culture tank by UFB
Case Study①mortality test in Red Snapper tank for Market

Circulating UFB water by Ultra Pump in culture fish tank,
improved water quality & mortality rate (“0” death)Study
Subject: Culture fish tank/Red Snapper
Content: Check for improved mortality rate
while cultured
Method: Circulate culture water by Ultra Pump
Continuous run 8 hrs./day avg.
Evaluation: Death count with/without UFB
Period: December, 2018 - April, 2019
Result: Without UFB - “2” deaths
With UFB- “0” death

By improving water quality and stabilizing dissolved oxygenlevel, “death” was prevented6Thawing Tuna with UFB water
Case Study②

Thawing frozen tuna in the Fish Market

Able to keep its bright color and freshness by thawing Tina withUFB water
Study
Subject: Frozen tuna
Method: Quality check after thawing
tuna with nitrogen UFB added
tap water

UFB

Tap Water

Evaluation: Comparing freshness ofthawed tuna by tap water andtap water with added nitrogen
UFB
Result:Oxidation controlled by UFB・Improved brightness in red color・Chefs evaluated that thefreshness lasts longer・No change in taste or smell withUFBNo negative effect on taste or smell, but keeps freshness and bright red color in Tuna 7

Improving mortality rate in young shrimp culture tank by UFB
Case Study③

Culturing young shrimp（Inland aquaculturing）

Reduced mortality rate of shrimp in half by setting a UFB pump in the tankStudy
Subject: Young Shrimp
Method: setting UFB pump incirculating system
Evaluation: 1 tank out of 6 is setup
with UFB (5 tanks with regularsystem). Comparing mortality
rate and dissolved oxygen level

Shrimp culturing process
Baby shrimp（0.8mm）

Test Period（2 mon.）Young Shrimp（20cm）
Spawn twiceMoved to another tank
Until fully grown

Ship to another culture
supplier

Period: about 2 months. （spawn season
early Oct. – Dec.)

BackDepth of the tank：35ｃｍ
Water vol. of the tank ：11.55㎥
Shrimp qty. per tank: about 500（male165, female 335）

5 regular tank

1 UFB

UFB Ultra
Pump

Filtering
System8

Improving mortality rate in young shrimp culture tank by UFB
Case Study③

Culturing young shrimp（Inland aqua culturing）Result

Result １
Reduced youngshrimp mortality rate
Regular tank:10death/mon./tank↓UFB tank: 4〜5 death

Female spawn 600 eggs
（9,000 Yen） twice a year

Omits 18,000 yen loss
per female shrimp

Result ２
Increased dissolved
oxygen level
Regular tank: 4〜5ppm↓UFB tank: 8ppm
※Oxygen level went up in UFB tank,despite water being replaced once aweek in regular tanks

Result ３
Eats better
Cause of eating better isthought to be the highoxygen level. Time was
not available to measure
the difference in thegrowth speed.

Already existing synergy effect with aeration

2MPa 2l pump（about 11t and only 2 l/min.）
Can improve culturing environment of
11.55㎥ tank9Mechanism of faster growth in aquaculture by UFB①
UFB enables sea water to dissolve oxygen 5 times the saturated amount,improving issues with deprived oxygen
Easy to deprive oxygen in the tank

EatingConsumesoxygen••••

Increased
Ammonia

When fish is vibrant,
Ammonia isdischarged by gills
respiration andexcrement

Consume Oxygen
Breaking it down

oxygen is easy to be
deprived in Tank

When deprived of Oxygen:

Microorganism
Improves
water quality

Fish do not eat
Leftover deposit and rot
Slow Ammonia decomposition
Low water quality makes fish sick→ Administrate Antibiotic→ Cost increase→ gives bad impression with drugalso

Consumeoxygen

Oxygen deprivation→less food eating→leftover deposits alongwith excrement

Deposit

Increased oxygen 5 times thesaturated amount

Consume oxygen
Breaking down leftoverand excrement

Increase appetite, promotes
growth, and improved water quality
10
reduces death in the tank

Mechanism of faster growth in aquaculture by UFB②
Nano mm UFB, can void cap phenomenon situation by its small size

Aeration ＝ Common process to increasing oxygen in tank
Problem with aeratingWith micro bubble•

Fish normally
avoids being inbubble curtain
The bubble bigger
than the space
between cilia,
covers the gill up＝cap phenomenon

Micro sized bubble(white in appearance)•

Fish swallows the bubble thatstays in water for a few min.
and spread•

Gill’s cilia spacing is a few

hundred nano
１ micro =1,000 nano
the bubble clogs up the gill(cap phenomenon)

Cilia spacing is a
few hundred nano

Cap phenomenon
prevent fish from gillbreathing to weaken！

UFB bubble is smaller than the space between cilia, avoids cap
phenomenon and provide oxygen.

11Mechanism of faster growth in aquaculture by UFB summary
UFB enables sea water todissolve oxygen 5 times thesaturated amount, improving
issues with deprived oxygen（UFB’s gas solubility effect）

Biofilm(slime＝germs nest)isdestroyed by UFB bubble
bursting to keep the highquality water（UFB’s power to physical destroy）

Ultra fine bubble is smallenough that do not allow cap
phenomenon（UFB’s characteristics）

Activating the fish’s
physiology and promotes itsgrowth（ UFB’s physiology vibranteffect ）All of these mechanism, made it possible for UFB toReduce culture time by half and enlarge the size by 1.5 times
12And The taste!
UFB ion decomposes ammonia in sea water to change it into amino group

When UFB bursts, OH radical is generated.
The chemical reaction between ammonia and OH radical creates aminogroup which is base of amino acid.
Ammonia

OH radicalAmino group

WaterHH
AmmoniaHigh
Toxicity

AmmoniaHigh
Toxicity
Amino
group

Amino Acid

When UFB bubble bursts,
OH Radical is generated

Amino acid is need nutrients that the body needs to functionAmino acid increases muscle in culture fish, and adds
flavor when deposits in the muscle
13

4 Merits by aqua culturing with UFB

Culturing time
reduced by promoted
growthImproved water
quality by UFB’s
traits to able tophysically destroy

Improved quality byincreased dissolved
oxygen level

Better flavor withadded amino acid

Just by adding UFB function to the water,Reduce cost & Increase profit
14

UFB Case Study in Agriculture

15

UFB case study in agriculture
Case Study①

Tomato in Green house（soil culture）

Just by irrigating with UFB water, Increase harvest and quality

Study
Subject: Tomato
Content: Compare the tomato
growth with “regularwater” and “UFB water”

irrigating

Method: Irrigating with UFB water（1,000L/10a）

UFB Irrigation: once per 10-14 days
Diluted UFB water isirrigated at the root
Evaluation: Comparing the harvest in
1 test area to other normallyoperated 2 areas .
(12.5kg/container)

３０アール30a
Farm ファーム熊本
Kumamoto

Test
試験区
Area
１０アール
10 a

Normal
慣行区
１０アール

Area
10 a

Normal

慣行区
１０アール

Area
10 a

Period: Nov. 2018～Apr. 2019
*The firm purchased 5 ultra pumps afterthe experiment period

16

UFB Case Study in Agriculture
Case Study①

Tomato in Green house（Result）✓ １６５％ increase in 8 times harvesting (avg.), during the test period✓ Ave. size of tomato is bigger and more uniformed in appearance✓ The red color is bright and taste well. The rate of clearing thestandard to be sold is increased.

＝＞The firm purchased 5 pumps after the experiment
ResultFirst UFB Irrigation on 11/15, started 3 weeks before the harvesting

Test Area(10a）Reg. Area（20a avg.）

Datecontainerqty.

12/9
12/12
12/1512/18
1/2
1/4
1/7
1/13

19.3
15.3
14.6
7.3
32.6
20
29.3
24

241
191
183
91
408
250
366
300

8
12.8
9.6
4.8
16.6
1020
16.6

100160
120
60
208
125
250
208

Test/Reg. area
Rate of increase
（％）
241%
120%
152%
152%
196%
200%
147%
145%

Period Avg.

162.4

2,030

98.4

1,230

165%

weight（㎏） container qty.

weight（㎏）17

UFB Case Study in Agriculture
Case Study②

Cherry Tomato in Green house（Hydroponic/Result）
品種✓ Able to harvest one week early✓ Increased harvest by 31％ avg.
✓ Result varied by the brand.
But most increased in harvest

ＵＦＢ有慣行

増減

Pomodoro

7,710

7,600

101%

Collet Long

20,22020,58098%

Hana Seven

11,540

8,210

141%

Black Cherry

22,940

8,710

263%

Green Zebra

26,00022,300117%

Total（ｋｇ）

88,41067,400

131%

Feb.
25th

ＵＦＢ

ＵＦＢ

Tap

Tap

ＵＦＢ

ＵＦＢ

Tap

Tap

Mar.
25th18

Mechanism of increased harvest in agriculture by UFB①

Irrigating with UFB water, provides enough oxygen to the roots in growth suitabletemperature

19Mechanism of increased harvest in agriculture by UFB ②
Irrigating UFB water activates the root to increase production of growth hormone

Zeta potential-100mA

Nourishment
Absorption
bandRoot tipmeristem

20Mechanism of increased harvest in agriculture by UFB ③

Nitrogen level per 100g dried soil (mg)

Aerobic bacteria became vibrant and nitrogen became more effective and stays longer insoil when irrigating with UFB

Days**reference taken from Kochi University of Technology:
Efficient Nitrogen-Containing Fertilizer Delivery into Soils Mediated by Aqueous Solutions Containing Microbubble
21

Mechanism of increased harvest in agriculture by UFB（Summary）
Easy to seep through
UFB’s penetration makes
water and nutrients easy toreach the roots

Activates aerobic bacteria

The stem becomes sturdy
by increased conduits
Number of conduits increased byabsorbing more water and nutrients.
The stem grows sturdy and thick tohold many fruits

Activates fixed nitrogen

Nitrogen rate in soil is
improved and stays longer

Activates plants physiology
UFB stimulates the root to promotesecretion of growth hormone. The fruit
permeates to absorb nutrients that
increases its sugar content

Delivers nutrients such as
potassium to the rootsUFB attaches positive ion such as
potassium to its bubble. Potassium aimportant nutrients to promote plants
growth and fulfil the fruit. Delivered
potassium promotes the growth andmake it fruitfulSpecial mechanisms make it possible

For only water and air to increase harvest
22