Disseminating Alternative Methods in Brazil
and South America: Education and Training for
Animal Replacement in Science
Laboratory of Education and Research in
Pharmacology and Cellular Toxicology, LPCTTeam
EDUCATION/TRAINING

Dr. Marize Campos Valadares
marizeufg@gmail.com@marize.campos.valadares

Pharmaceuticalindustry clusters: 25Industries.
Teuto/Pfizer of Brazil,
biggest in SouthAmerica
Agrochemical cluster:pesticides

Goiânia (1.3 million)
Capital of Goiás
State (6.5 million)

UNIVERSIDADE FEDERAL DE GOIÁS
23,000 students
150 undergraduate courses
85 Master and Doctorate Programs

“ALTERNATIVE METHODS” “INNOVATIVE METHODS”
forPHARMACEUTICAL INNOVATIONUNIVERSIDADE FEDERAL DE GOIÁS-UFG

School of Pharmacy
Goiânia

Brazilian Network for
Alternative Methods

PhD student, Renato Ávila
Young Researcher Americas
The 2017 Lush Prize

IN VITRO METHODS FOR
PHARMACEUTICAL PRODUCTS:
Efficacy/Safety

The current paradigm is ingrained in students and we believe that
education/training for 21st Century Science is the basis for a shift in thinking
beyond animal models. I have, therefore, been introducing new in vitro
scientific concepts to undergraduate and graduate students of innovation inPharmaceutical Sciences. Professionals, scientists and regulators from Brazil
and other South American countries have also been trained in non-animalmethods by our team.

BRAZILIAN CONTEXTNational Council for the Control ofAnimal Experimentation - CONCEANORMATIVE RESOLUTION nº18/2014
Recommend 17 alternative methods to animal experimentation

TRAININGNORMATIVE RESOLUTION nº 31/2016
Recommend 07 alternative methods to animal experimentation
BRAZILIAN HEALTH REGULATORY AGENCY (ANVISA)
Collegiate Board of Directors Resolution (RDC) no. 35/2015:
recognize the methods approved by CONCEADEADLINE2019

EDUCATIONUNDERGRADUATION:-Courses to Pharmacy and Biomedicine students:“Introduction to Toxicology” and “Applied Toxicology”.
-UFG Toxicology League: “Toxicology in the 21stCentury”.
GRADUATION: (MSc and PhD students of Life Sciences):-Multidisciplinary Master and Doctorate Programs: PharmaceuticalSciences, Health Sciences, and Pharmaceutical Innovation (networkProgram involving 4 North Brazilian States).
- Courses: “Alternative Methods to Animal Experimentation”, “Toxicology
for the 21st Century: an alternative to animal testing”, “Special topic inalternative methods”.

TRAININGLast 18months
LECTURES AND HANDS-ON EXERCISES: CBTOx: OECD guidelines (OECD437/2017, OECD 432/2004, OECD 431/2016, OECD 491/2017 and HETCAM.
-Flow Cytometry applied to Toxicology In Vitro;-Skin Sensitization-Epigenetic and Toxicology-“I ABCF – UNIVALI Workshop”, Itajai

REGIONAL PLATFORM OF ALTERNATIVE METHODS OF MERCOSUL(PREMASUL): 3 editions: Lectures and Training in Brazil and Uruguay
OECD 442C, OECD 442D, OECD 442D, OECD 442E, GARD

Harmonization of theInternational regulatory requirement
Optimizing of the in vitro tests implementation:Series of training videos in Skin Sensitization:
English, Portuguese, Korean and Mandarin
In Chemico Skin Sensitization:
Direct Peptide Reactivity Assay(DPRA) – OECD 442C

First video training: DPRA

On the last 18 months, a total of 140 participants from different categories ofindustry and academia were trained in animal-free testing practices.
During the past 18 months, over 170 undergraduatesand more than 45 MSc/PhD students were introduced
to new paradigms in Toxicology for a new Science.
Over the same period, I have supervised 4 scientificinitiation undergraduates, 4 MSc and 5 PhD students
and 2 post-doc fellows, yielding a total of almost 100
multidisciplinary human resources supervised sincethe 2004 foundation of our Laboratory.

BRAZILIAN SCENARIUM“BRAZIL´S SAD CHOICES”

Political turmoil X Economy
Institutions in Argentina and Brazil are struggling
to maintain their funding and talent.

RESEARCHING IN BRAZIL:- Expensive- Takes more time
- Customs issues: dificult to buy
cell lines, tissues, reagents ...

PERSPECTIVES- Science communication about our work in Innovative
Methods in Brazil and South America;- Workshops and training: lung toxicity;...

- Training videos

Disseminating knowledge in animal-free practices!

Obrigada!!!!!!!