INSTRUCTIONSFOR USECELLDISC
Multi Layer Device(1 - 40 Layers)

SIMPLIFY YOUR CELL CULTURE
WORK PROCESS

1/ SINGLE LAYER CELLDISC

CELLDISCSINGLE LAYER

The Greiner Bio-One CELLdisc is a ready-tostart, multi layer device, as easy to use as aT-flask. The innovative ergonomic CELLdisc
design provides a versatile system for thepropagation of adherent mammalian cells
from research scale to industrial batches.
It is available either with the standard tissue
culture surface (TC; red screw cap) or theAdvanced TC surface (Adv. TC, blue screw cap)
identical to all Greiner Bio-One cell culture
products to assure consistent performancefrom lot to lot and from format to format.

The single layer CELLdisc (CD1), in contrast to
the larger multi layered versions, allows easy
monitoring of cell growth, cell morphology andconfluency. As conditions within the single
layer CELLdisc will be identical to those withinlarger units, the CD1 can be used to anticipate
when media changes are required and harvesting is recommended in any larger units
being cultivated alongside the CD1. To ensurethat the ambient conditions for the reference
CD1 and the multi layer CELLdisc are absolutelyidentical, the CELLevator allows storage of thesingle layer CELLdisc on top of the CELLdisc
production unit. To maintain the accustomed
quality and efficiency of cell culture, customers are advised to test Greiner Bio-One
systems under the conditions defined intheir own protocols.

INTENDED USE
General laboratory product for cell culture to
be used by qualified personnel in a laboratoryenvironment. For research use or for further
processing. Not for diagnostic use or direct
administration into humans.

1Unpack the single layer CELLdisc and place
it in a laminar air flow cabinet in order to workin sterile conditions.
Prepare cell suspension in accordance withthe concentration (cells/cm2) used with otherdisposables for adherent cell culture.

2RECOMMENDED
HANDLING

CELLDISC MULTI LAYER
DEVICE (1 - 40 LAYERS)

Unscrew screw cap and transfer the cellsuspension directly into the CELLdisc using
the large opening port either by pouring orpipetting.
Firmly tighten the screw cap onto the CELLdisc to close it.

3Tilt the single layer CELLdisc gently from one
side to the other to assure that media andcells distribute evenly.

Integrated gas channel:

Supports optimal
airflow
Filter port:

For pressure
equilibration

Large opening port:

For easy filling
Connecting channel:

For liquid transfer

WARNING

Rim assures equal
thermal distribution

Figure 1: CELLdisc with 1, 16 and 40 layers

Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

The media or cell suspension should not touch the filter. If the filter has absorbed any
fluid, this will inhibit any gas transfer into and out of the CELLdisc. In this case the disposable
has to be discarded and a new single layer CELLdisc has to be used.

Figure 2: General CELLdisc details

2 / 12F000360 EN [Rev.05, 07.2024]

Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

3 / 12F000360 EN [Rev.05, 07.2024]

2/ CELLDISC 4 - 24 LAYERS

RECOMMENDED
HANDLINGUnpack the CELLdisc and place it in a laminar
air flow cabinet in order to work in sterile
conditions.

3Prepare cell suspension in accordance withthe concentration (cells/cm2) used with otherdisposables for adherent cell culture. Hold theCELLdisc at an angle of 30°.

2

Hold the CELLdisc with the screw cap at aposition of approximately 105° for righthanded users or 255° for left-handed ones.

3To simplify filling procedure,

Unscrew screw cap and add the cell suspension either by pouring or pipetting directly into
the CELLdisc using the large opening port.
The media will fill the topmost layer first andthen move slowly to each layer underneath.

the CELLdisc can be positioned on the4

Wait until the liquid is distributed into theindividual layers still keeping the CELLdisc in the indicated position before firmly
tighten the screw cap to close it.

5

To start liquid equilibration lay down CELLdisc
horizontally and turn it to assure that themedia and all layers are in contact throughthe connecting channel.

CELLstage filling device (see orderingRECOMMENDED
HANDLING

1information).

WARNINGTo obtain equivalent cell growth in all layers, formation of air bubbles must be avoided during
CELLdisc processing. Therefore, an exact angle of 30° and a specific position of the connecting
channel must be maintained during filling of 4-24 layer CELLdisc. This handling procedure
guarantees that the pressure is equalized through the connecting channel (indicated in blue inchapter 2, step 3) without contact with the filled in liquid. Thus, the air does not flow through theliquid and does not cause foaming. In addition to the filling process, any generation of air bubbles
should be avoided. Vigorous shaking of the CELLdisc is not recommended. Larger volumes ofliquids should be mixed outside the CELLdisc and then added to the disposable as described
below. Small amounts can be pipetted directly into the CELLdisc and then distributed to all layers
by repeating the equilibration process.

Unpack the single layer CELLdisc andWARNING
place it in a laminar air flow cabinet inwork
in sterile
conditions.
The media or cell suspension should not touch theorderfilter.toIf thefilter
has absorbed
any fluid,
this will inhibit any gas transfer into and out of the CELLdisc. In this case the disposable has tobe discarded and a new CELLdisc has to be used.

1Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

5 / 12F000360 EN [Rev.05, 07.2024]

32/ CELLDISC 4 - 24 LAYERS

8

NOTE: Small volumes of liquids(e.g. trypsin) may accumulate in theupper layers during filling and not flow

3From this position raise the CELLdisc uprightand place the disposable on a horizontal
surface inside an incubator.
Proceed with the cultivation based on theappropriate protocol.

through the whole filling channel.
To guarantee equal distribution, theliquid must be in contact with all layers

RECOMMENDED
HANDLING

RECOMMENDED
HANDLING

2/ CELLDISC 4 - 24 LAYERS

through the filling channel before
equilibration process is initiated.
Therefore the CELLdisc must be posi-3

tioned horizontally with the opening
port at the lowest position.

NOTE: During transport, tilt theCELLdisc slightly backward to assurethat there is no liquid contact with thefilling channel or accidental media
flow to another layer.

689The media will now distribute equally between
all layers.

To stack individual CELLdisc or a single layer
CELLdisc on top of a production CELLdisc
use the CELLevator.
More details on CELLevator can be found on
the reverse side.

7810Turn the CELLdisc to disconnect media flowfrom the filling channel.
Do not rotate the CELLdisc any further as this
could lead to contact between liquid and filter.

Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

6 / 12F000360 EN [Rev.05, 07.2024]

Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

For liquid removal, unscrew screw cap and tilt
the CELLdisc slowly 90° with the large opening
port at the lowest possible position and pourout the media or use a pipetting system.

7 / 12F000360 EN [Rev.05, 07.2024]

123

3/ CELLDISC 40 LAYERS

As with the CELLdisc 4-24 layers, air bubble
formation must be avoided during processing
to obtain even cell growth in all layers. While
the position of the screw cap/filling channel
and the general filling procedure is identicalto recommended handling of CD4-24 a smaller
angle of 20° must be maintained during fillingof a 40-layer CELLdisc.

3This handling procedure guarantees that thepressure is equalized through the connecting
channel (indicated in blue) without contact
with the filled in liquid. Thus, the air does notflow through the liquid and does not create airbubbles.

4

the liquid must be in contact with alllayers through the connecting channel
before equilibration process is initiated.
Therefore the CELLdisc must be positioned horizontally with the opening port
at the lowest position.

5

To simplify filling procedure,
the CELLdisc can be positioned on theCELLstage filling device (see orderinginformation).

3

NOTE: To guarantee equal distribution,

RECOMMENDED
HANDLING

RECOMMENDED
HANDLING

3/ CELLDISC 40 LAYERS

Turn the CELLdisc to disconnect media
flow from the filling channel.

From this position raise CELLdisc upright andplace the disposable on a horizontal surface
inside an incubator.
Liquid removal is identical to the recommended handling of CELLdisc 4-24 layers.

To start liquid equilibration lay down theCELLdisc horizontally and turn it to assurethat the media and all layers are in contact
through the central filling channel.
The media will now separate equally between
all layers.

Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

8 / 12F000360 EN [Rev.05, 07.2024]

Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

9 / 12F000360 EN [Rev.05, 07.2024]

PRODUCTOVERVIEW

4/ CELLDISC 4 - 40 LAYERS

FOR FURTHER INFORMATION AND/OR SAMPLE ORDERING
PLESAE VISIT OUR WEBSITE OR CONTACT US.

CELLdisc with TC surface

RECOMMENDED
HANDLING

HARVESTING OF CELLS
The protocol for harvesting cells from amultilayer device such as CELLdisc which
does not allow for direct pipette accessvaries slightly from your standard protocol.
Especially as the detachment of cells canbe visualized microscopically only for thebottom layer of a CELLdisc with one, four or
maximum eight layers.
This cell harvest protocol refers to standard
techniques and specific suggestions to gain
maximum cell yields.
In general, we recommend using the same
dissociating solution and concentrations
for enzymatic detachment that is used toharvest these cells from a standard Greiner
Bio-One cell culture disposable. As thesurface treatments and basic materials
are identical for these vessels, standard
protocols can be converted.

Sterility SAL 10-6

dissociation agent per layer. For a 4-layer
CELLdisc this results in 28-40 ml which
has to be equilibrated as described above.

Item No.

Incubate CELLdisc at 37 °C and 5 % CO2 for
3-5 minutes. Tapping CELLdisc from the side
can accelerate cellular detachment. Strong
adherent cells might need longer incubation
or stronger tapping. Stop the enzymatic
reaction by adding the same amount ofserum-containing media or an appropriate
inhibitor to the CELLdisc, equilibrate theliquids and mix the solutions by gently tilting the CELLdisc back and forth. Thereaftercell suspension can be harvested for further
processing by pouring or aspiration.

Surface

Growth area
[cm³]

Min. working
volume[ml]Max. working
volume [ml]

Ø [mm]

Bag [pcs]

6781011TC

2501570200

8678104

4TC

1.000

60

280

200

4678108

8TC

2.000

120

560

200

3678112

12TC

3.000

180840200

2678116

16

TC

4.000

240

1.120

200

2678124

24

TC

6.000

360

1.680

200

2678140

40TC

10.000600

2.8002001CELLdisc with TC surface
Sterility SAL 10-6

If removal of the enzymatic dissociation
agent is desired, then spin cell suspension
at 100xg for 5 minutes. Remove the dissociation agent containing media and resuspendcell pellet in fresh media.

Item No.

Layers

Surface

Growth area
[cm³]

Min. working
volume[ml]Max. working
volume [ml]

Ø [mm]

Bag [pcs]

678901

1Adv. TC

250

1570200

86789044Adv. TC

1.000

60280

200

46789088Adv. TC

2.000

120560200367891212Adv. TC

3.000

180840200

2678916

16Adv. TC

4.000

240

1.120200

2678924

24

Adv. TC

6.000

360

1.680

200

2678940

40Adv. TC

10.000600

2.8002001CELLdisc with external filter
Sterility SAL 10-6, triple packed

The only adaption required is based on growtharea and cell numbers per layer. The use ofchelating agents such as EDTA in addition
to the enzyme (trypsin, papain etc.) may
improve cellular detachment. Remove cultivation media either by pouring or aspiration
as described above. Wash cells once withPBS or an equivalent buffer using approx.
20 ml per layer. Follow the same protocol
as for CELLdisc filling to distribute the buffer
through all layers. Then tilt the CELLdisc
slowly back and forth to gently rinse eachcell layer and remove all traces of media.
Remove PBS either by pouring or aspiration.
Thereafter add 7-10 ml of your enzymatic

Greiner Bio-One GmbH
Maybachstraße 2 / D-72636 Frickenhausen / Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

LayersAdv. TC on demand

Item No.

Layers

Surface

Description

6781xx-EXF

1-40TCCELLdisc with external filter

1-40

Adv. TC

CELLdisc with external filter

CELLdisc with closed filling caps
Sterility SAL 10-6, triple packed

Adv. TC on demand

Item No.

Layers

Surface

Description

6781xx-CF1

1-40

TC

CELLdisc with closed filling cap, single tubing and external filter

6781xx-CF24-40

TC

CELLdisc with closed filling cap, double tubing and external filter

1-40

Adv. TC

CELLdisc with closed filling cap, single tubing and external filter

4-40

Adv. TC

CELLdisc with closed filling cap, double tubing and external filter

CELLdisc Accessories

10 / 12F000360 EN [Rev.05, 07.2024]

Item No.

Description

MaterialMeasure [mm]Weight [kg]878072

CELLstage for CELLdisc with 4-24 layers

stainless steel

219x211x126

1.53878073

CELLstage for CELLdisc with 40 layers

stainless steel

311x211x141

2.29

11 / 12

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PHONEFAX
E-MAIL

+49 7022 948-0+49 7022 948-514
info@de.gbo.com

Devices of Greiner Bio-One are to be used by properly qualified persons only inaccordance with the relevant Instructions for Use (IFU), where applicable.
For more information contact your local Greiner Bio-One sales representative
or visit our website (www.gbo.com).
All information is provided without guarantee despite careful processing. Any liability, warranty
or guarantee of Greiner Bio-One GmbH is excluded. All rights, errors and changes are reserved.
If not stated otherwise, Greiner Bio-One GmbH has all copyrights and/or other (user-)rights inthis documents, in particular to signs such as the mentioned (word-picture-)brands and logos.
Any use, duplication or any other use of the rights of Greiner Bio-One GmbH is expressly prohibited.
Media owner: Greiner Bio-One GmbH / Represented by Managing Director Bernd Klingel.
The company is registered in the Commercial Register at the first instance courtin Stuttgart, HRB 224604 / VAT Number: DE812585719.

F000360 english [Rev.05, 07.2024]

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