CLINICAL STUDY BRIEF

Rotational TissueProcessors are 7x as
Efficient when comparedto Tidal Agitation
Tissue Processors
Rotational and Tidal Agitation Fluid Exchange in Comparison of Tissue Processors
Dr. Parvis Merati, Jared Boyd, Hueng Leong
Western Michigan University (WMU) Fluids Laboratory

Tissue processor design facilitates the replacement of water-based fixatives through dehydration, clearing,and infiltration resulting in a paraffin-infiltrated tissue sample. Traditional tissue processing is timeconsuming,
frequently taking up to 10 or more hours to complete a cycle. As a result, the tissue processing
step is often the most significant workflow bottleneck within the histology laboratory. Efforts to speed up
tissue processing have resulted in an innovative technology known as rotational agitation (vs. traditional
processing of tidal agitation). In rotational tissue processors (RTP), rotational agitation is performed atconventional processing temperatures and without microwaves, offering the advantages of traditional
processing in a fraction of the time. This study focused on a series of measurements designed to compare
the two methods and clearly show the benefits of rotational agitation by providing an overall seven-foldimprovement in fluid exchange efficiency.

The Revos tissue processor’s design is optimized
for both routine and rapid processing.
The Rotational tissue processor (RTP) is superior in all ways to thetidal agitation processor. The RTP processor is seven times more
efficient than the tidal agitation processor at passing fluid. It is also
5 times large in turbulence and vorticity, which could be used asa measurement of the amount of mixing that occurs within
the cassettes.

Rapid processing

Revos workflow-enhancing
tissue processor

The Revos tissue processor’s unique,canted chamber enhances reagent
distribution, reduces tissue processing
time, and allows for high-quality processing results.

The Revos tissue
processor’s design
is optimized forboth routine andrapid processing.

Volume Flow Rate Calculations of a Cassette
Rotational Tissue Processor
MachineTidal Agitation Machine

Drainage Flow Rate

Drainage Flow Rate

Cassette Volume = 3721mm3

Cassette Volume = 3721mm3

Drainage Cycles = 8 cycles/min

Drainage Cycles = 0.05 cycles/min

Volume Flow Rate = 496 mm3/sec

Volume Flow Rate = 3 mm3/sec

Velocity Flow Rate

Velocity Flow Rate

Cassette Area = 709 mm2

Cassette Area = 709 mm2

Average Flow Velocity = 65 mm/s

Average Flow Velocity = 4 mm/s

Immersion Time = 40%/cycle

Immersion Time = 100%/cycle

Volume Flow Rate = 18434 mm3/sec

Volume Flow Rate = 2836 mm3/sec

Total Volume
Flow Rate = 18930 mm3/sec

Total Volume
Flow Rate = 2839 mm3/sec

Epredia RevosTissue Processor• Quality• Consistency• Ease of use

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Figure 1. It can be seen from thecalculations, that volume flow rate in
the Rotational tissue processor (RTP) is
far superior to the Tidal Agitation tissue
processor, and is almost seven times
larger. We could then imply that the RTP
processor is seven times as efficient as
the tidal agitation processor machine.