CLINICAL STUDY BRIEF

Rapid processingof fatty tissues
Tissue Processing using Epredia™ Revos™,
which does not require defatting pre-treatment,
is considered to speed up specimen preparationof fatty tissues such as breast specimens.
Investigators examined the defatting effect ofRevos, an automated tissue processor witha canted chamber, and its usefulness for thepreparation of embedded samples of fatty(adipose) tissue.
High-quality FFPE specimens of fatty
tissue are possible when using a
Revos tissue processor.

Defatting Effect ofAutomated Tissue Processor
with Canted Chamber (Revos)
Shinya Abe and Tomohiro Yoshinaga,
Epredia Pathology Business Department,
PHC Corporation; Masanobu Kitagawa,
Miori Inoue, and Kohei Yamamoto,Department of Comprehensive Pathology,
Graduate School of Medical and Dental
Sciences, Tokyo Medical and Dental
UniversityA

B

C

Paraffin blocks of breast tissue
specimen, prepared with:A
B
C

Conventional tissue processor
without defatting pre-treatment
Conventional tissue processor
with defatting pre-treatment
Revos (without defatting pre-treatment).

When the specimen was processed using
Revos, the characteristic yellow tone of fat
tissue faded and the tissue was transparent,
even without defatting pre-treatment.

The automated tissue processor, Epredia Revos, used in this study, hasa canted chamber, allows sufficient penetration of the process reagent intospecimens repeatedly immersed and removed from the reagent solution.
This study demonstrates that this method facilitates the penetration of reagent
solutions into the tissues and promotes the replacement of reagents.

Epredia Revos
Tissue Processor• Quality• Consistency• Ease of use

Revos workflow-enhancing
tissue processor

Rapid processing
The Revos tissue processor’s unique,canted chamber enhances reagent
distribution, reduces tissue processing
time, and allows for high-quality processing results.
The Revos processor has a canted chamber, which allows
sufficient reagent penetration into specimens repeatedly
immersed and removed from the reagent solution.

Specimens prepared using theEpredia Revos tissue processor
showed fewer cracks even without
defatting pre-treatment.

ABCD

It is known that this method facilitates thepenetration of reagent solutions into the tissues
and promotes the replacement of reagents.
The study suggests that automated tissue processors witha canted chamber provide appropriate agitation of tissue
from which water trapped in fat is replaced by a penetrating
reagent and removed, resulting in adequate
tissue dehydration.

This section of breast tissue prepared with:
A, B Conventional tissue processor(without defatting pre-treatment).
C
Conventional tissue processor(with defatting pre-treatment).
D
Epredia Revos tissue processor(without defatting pre-treatment)

H&E staining was performed on each specimen. The specimen prepared using Revos
had only minor cracking, resulting in better orientation of the entire specimen.
H&E staining of a breast tissue specimen
prepared with:AB

ABCTo learn more about the Epredia Revos instrument’s
unique processing solution visit epredia.com orcontact your sales representative for more information© 2022 Epredia. All rights reserved. All trademarks are the property ofEpredia and its subsidiaries unless otherwise specified. M43033 R0622

C

Conventional tissue processor(without defatting pre-treatment).
Conventional tissue processor(with defatting pre-treatment).
Epredia Revos tissue processor(without defatting pre-treatment)