Poster Instructions for Ag-ESD Symposium 2016
●Registration（ポスター申し込み方法）① Please register with the following website Registration Form by July 14, 2016.
URL：http://www.nourin.tsukuba.ac.jp/~agesd/ag-esd2016.html②Please send your "Poster Abstract" as a Word format file by E-mail toagesd@nourin.tsukuba.ac.jp no later than July 14, 2016. Do not send "pdf file".
(See Abstract Instructions below.)●Poster Abstract Instructions and Example（ポスター要約作成と見本）
Please follow these instructions for preparation of a one page abstract of your poster
presentation.
1.

LanguageEnglish Only

2.

Abstract format (Refer to example below)

Title of Presentation (Bold 14 pt Times New Roman)
Author name, affiliation and body of abstract should follow the title without bold type
and with a 12 pt Times New Roman fontName(s) of author(s) should include the Given Name first, Middle Name next
and FAMILY NAME last with Family Name in All Capital Letters
Your abstract should be single spaced.
Use right justification with a 3.0 cm top margin and2.5 cm margins for the bottom and both sides.
Length of the summary should not exceed one page.

(Example)

Genetic Studies on Tick Ecdysteroid Regulation for Development ofSustainable Tick Control Strategies
Mari H. OGIHARA1,2 and DeMar TAYLOR3
1Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan

2Fellow of the Japanese Society for the Promotion of Science
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan

3Ticks are exoparasites of mammals and important pest of livestock production. Presently,tick management is limited to the use of acaricides that provide great advantages to livestock
production but also ticks develop resistant against acaricides. Development of alternative
ways for sustainable livestock production is required. The knowledge of tick ecology andphysiology is important to construct strategies for integrated tick management. Tick molting
and reproduction require blood feeding and are under endocrinological regulation. Therefore,
our studies focus on the roles of blood feeding and mating in the regulation of theseendocrinological mechanisms.
Viable egg production requires both engorgement and mating, but the distinct roles ofthese two stimuli are not understood. The soft tick Ornithodoros moubata provides an
excellent model to study the separate roles of feeding and mating because both virgin andmated females engorge but only mated females produce viable eggs. Ecdysteroid titers
significantly increased in only mated females and remained low in virgin females. In addition,
both mated and virgin females showed up-regulation of EcR and RXR hormonal receptors
immediately after engorgement. Both mated and virgin females showed Vg expression during
an early phase (3 to 10 days) after engorgement, whereas only mated females showed
increased Vg expression during the late phase (12 to 20 days). Whole mount in situ
hybridization revealed the main site of Vg expression is the midgut during the early phase
while the fat body enlarges and shows strong Vg expression during the late phase in only
mated females. Therefore, feeding stimulates Vg expression in both virgin and mated females
while mating is required for Vg up-regulation in the late phase. Furthermore, mating appears
to induce secretion of ecdysteroids after engorgement and high titers of ecdysteroids upregulate Vg expression in mated females leading to mature egg production. This two phaseregulation of vitellogenesis in O. moubata reveals the importance of investigating theseparate roles of nutrition and mating in arthropod reproduction. Understanding thesemechanisms may lead to the development of better strategies for the use of hormone agonists
in controlling tick populations.