YK070 Human Chromogranin A EIAYANAIHARA INSTITUTE, INC.
SDS No.000７0-011
Jan. 31, 2024
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Safety Data Sheet
１． PRODUCT AND COMPANY IDENTIFICATION
Product Name:
Product number:
Manufacturer:First issue:

January 25, 2008

Human Chromogranin A EIAYK070
YANAIHARA INSTITUTE, INC.
Address:
2480-1, Awakura, Fujinomiya-shi
Shizuoka, Japan 418-0011
Tel:+81-544-22-2771(Japan)
Fax:+81-544-22-2770
E-mail:ask@yanaihara.co.jp
Eleventh issue: January 31, 2024

２． HAZARDS IDENTIFICATIONGHS classification
Classification of the substance or mixture 4), 7), 8), 9)
Acute toxicity - Inhalation (Dusts/Mists)
Skin corrosion/irritation
Skin sensitization
Germ cell mutagenicity
Carcinogenicity
Serious eye damage/eye irritation
Specific target organ toxicity (single exposure)
Category 1 respiratory system
Category 2 blood system
Category 3 respiratory tract irritation, narcotic effects
Specific target organ toxicity (repeated exposure)
Category 1 respiratory system
Aquatic environment (acute hazard)
Aquatic environment (long-term hazard)

Pictograms

Signal word

DangerHazard statements
H314 - Causes severe skin burns and eye damage

Category 4
Category 1
Category 1
Category 2
Category 2
Category 1
Category 1, 2, 3Category 1
Category 2
Category 2

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H317 - May cause an allergic skin reaction
H318 - Causes serious eye damage
H332 - Harmful if inhaled
H335 - May cause respiratory irritation
H336 - May cause drowsiness or dizziness
H341 - Suspected of causing genetic defects
H351 - Suspected of causing cancer
H370 - Causes damage to the following organs: respiratory system
H371 - May cause damage to the following organs: blood system
H372 -Causes damage to the following organs through prolonged or repeated exposure:respiratory system
H401 - Toxic to aquatic life
H411 - Toxic to aquatic life with long lasting effects
Precautionary statements-(Prevention)
Obtain special instructions before use.
Do not handle until all safety precautions have been read and understood.
Do not breathe dust/fumes/gas/mist/vapors/spray.
Wash face, hands and any exposed skin thoroughly after handling.
Do not eat, drink or smoke when using this product.
Use only outdoors or in a well-ventilated area.
Contaminated work clothing should not be allowed out of the workplace.
Avoid release to the environment.
Wear protective gloves/protective clothing/eye protection/face protection.
Use personal protective equipment as required.
Precautionary statements-(Response)
IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
IF ON SKIN: Wash with plenty of soap and water.
IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin withwater/shower.
IF INHALED: Remove person to fresh air and keep comfortable for breathing.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if
present and easy to do. Continue rinsing.
If exposed or concerned: Get medical advice/attention.
Immediately call a POISON CENTER or doctor/physician.
Call a POISON CENTER or doctor/physician if you feel unwell.
If skin irritation or a rash occurs: Get medical advice/attention.
Wash contaminated clothing before reuse.
Collect spillage.
Precautionary statements-(Storage)
Store locked up.
Store in a well-ventilated place. Keep container tightly closed.
Precautionary statements-(Disposal)

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Dispose of contents/container to an approved waste disposal plant.
OthersOther hazards

Not available

Other reagents may be harmful if inhaled and ingested.
３． COMPOSITION, INFORMATION ON INGREDIENTS
Product Name
Human Chromogranin A EIA

May cause eye and skin irritation.

CAS Number
None

Kit components:
1)

No.

Component
Antibody coated plate

Quantity
1 plate2)
3)
4)

CgA Standard
Labeled antigen
Specific antibody

100 pmol
30 ng/vial
1 vial5)
6)

SA-HRP solution
Substrate buffer

12 mL
25 mL

7)
8)
9)

OPD tablet
Stopping solution
Buffer solution(Concentrated)

2 tablets
12 mL
12 mL

10)

Washing solution(Concentrated)

50 mL

11)

Adhesive foil

3 sheets

Chemical namePlate coated with goat anti rabbit IgG antibody①
Synthetic human CgA(344-374) ②
Biotinylated human CgA(344-374) ③
Rabbit anti human CgA(344-374) antibody ④
EDTA2Na⑤
HRP labeled Streptavidin⑥
Hydrogen peroxide ⑦
Citric acid, monohydrate ⑧
Disodium hydrogenphosphate 12-water ⑨
o-Phenylenediamine dihydrochloride⓾
Sulfuric acid (1M) ⑪
Phosphate buffer with non specific reaction
blocker ⑫
EDTA2Na⑤
Sodium chloride ⑬
Polyoxyethylene sorbitan monolaurate(Tween20) ⑭

Wt%

CAS No.

Chemical Formula

17.7%

6381-92-6

C10H14N2Na2O8.2H2O

0.015%
0.7%
2.39%
13mg
9.69%

7722-84-1
5949-29-1
10039-32-4
615-28-1
7664-93-9

H2O2
C6H8O7・H2O
Na2HPO4・12H2O
C6H4(NH2)2 2HCL
H2SO4

3.7%
18%
1%

6381-92-6
7647-14-5
9005-64-5

C10H14N2Na2O8.2H2O
NaCl
C58H114O26

４． FIRST AID MEASURES
Inhalation:
Immediately remove victim to fresh air. Consult a physician if necessary.
Eye contact: Immediately flush eyes with flooding amounts of running water for at least 15
minutes. Consult a physician if necessary.
Skin contact: Immediately remove contaminated clothes and shoes, flush skin with plenty ofwater or shower. Wash contaminated clothing and shoes.
Consult a physician if necessary.
Ingestion:
Immediately seek medical attention.
５． FIRE FIGHTING MEASURES
Flammable properties:
Nonflammable
Extinguishing media:
Foam, Carbon dioxide, dry chemical powder, soil, water
Firefighting instructions:
May emit toxic fumes under fire conditions. Wear full fire fighting
protective equipment including self-contained breathing apparatus.
Do not contact to the components when extinguish fire.
６． ACCIDENTAL RELEASE MEASURES
Personal precautions:Remove all ignition sources and ventilate. Wear suitable protective
equipment. Avoid contact with skin and eyes. Keep off except persons
concerned.

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Environmental precautions: Prevent spills from entering sewers, watercourses or low area, and preventfrom affecting environment.
Methods for Clean up:
In case of spill of liquid material, take up or cover spilled material withashes or other incombustible absorbents, and put in a container to be sealed.
After completely picked up, dispose. In case of spill of solid or powdermaterial, prevent causing dust, sweep and collect, and put in a container tobe sealed. Wash the spill site with water.
７． HANDLING AND STORAGE
Handling:

Storage:

Obtain a package insert before use.
Read all the cautions for safety in the package insert before use.
Avoid strong light.
Avoid contact, inhalation and swallow.
Use only in open air or ventilated area.
Prevent from entering eyes.
Ventilate the area to keep concentration in air below exposure limits.
Avoid inhalation of mist, vapor and spray of material.
Avoid contact with eyes, skin and clothing.
Do not smoke and eat while using this kit.
Wash hands thoroughly after handling.
Prevent from entering environment.
Handle materials with suitable protection.
Use suitable equipments.
Do not pipette by mouth.
Do not leak, overflow and scatter.
Do not fall down and damage.
Store away from sunlight in a cool and dark place at 36-47ﾟF (2-8ﾟC).

８． EXPOSURE CONTOROLS, PERSONAL PROTECTION
Engineering measures:

General ventilation and/or local exhaust ventilation as well as process isolationis necessary to minimize employee exposure and maintain exposure limits below
exposure limits. Equip eye flushing facilities and shower rooms near operating
place where this kit is handled or stored.

Control parameter:⑦ACGIH TLV(s);⑩ACGIH;⑪JSOH (Japan);
ACGIH TLV(s);TWA= 1 ppm
TWA=0.1mg/m3
OEL= 1 mg/m3
TWA= 0.2 mg/m3

Personal protection:
Respiratory protection; NIOSH and MSHA approved respirator.
Hand protection;
Suitable impervious gloves.
Eye protection;
Suitable safety glasses (goggles).
Skin protection;
Suitable protective clothing.

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Others:Wash hands thoroughly after handling materials.

９． PHYSICAL AND CHEMICAL PROPERTIES
Component
AppearancepH
Melting point
Boiling point
Flash point
Explosive
limitsVapor
pressure
Vapor density(air=1)
Specifics
gravity
Solubility inwater
Decomposition
temperature

1)

2)

3)

4)

5)

6)

7)

8)

9)

10)

Colorlessplate

White color,lyophilized
powderWhite color,lyophilized
powderWhite color,lyophilized
powderOrange color,
LiquidColorlesstransparent,
LiquidWhitetabletColorlesstransparent,
LiquidLight yellow
color, Liquid

Colorlesstransparent,
Liquid

N/A
N/A
N/A
N/A

N/A
D/N/A
N/A
D/N/A

N/A
D/N/A
N/A
D/N/A

N/A
D/N/A
N/A
D/N/A

D/N/A
N/A
D/N/A
D/N/A

5.0
N/A
D/N/A
D/N/A

N/A
D/N/A
N/A
D/N/A

<1.0
N/A
D/N/A
D/N/A

7.0
N/A
D/N/A
D/N/A

D/N/A
N/A
D/N/A
D/N/A

11)
Colorlesstransparent
Polymer
sheet
N/A
N/A
N/A
N/A

N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

N/A

N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

N/A

N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

Insoluble

SolubleSolubleSolubleMixableMixableSolubleMixableMixableMixableInsoluble

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

D/N/A

N/A

N/A.; Not applicable;
D/N/A; data not available

10． STABILITY AND REACTIVITY
Chemical stability:
Product is stable under normal handling.
Shelf life:
Stable up to 12 months after manufacturing.
Hazardous polymerization:Will not occur.
Conditions to avoid:
Extremes of temperature and direct sunlight.
Incompatibility with other materials: Alkaline substances, strong oxidizing agents.
Hazardous decomposition products: Sulfur oxides (SOx), Carbon monoxide(CO), carbon dioxide(CO2),
Nitrogen oxides (NOx), Hydrogen chloride(HCl) gas.
11． TOXICOLOGICAL INFORMATION
Information as the mixture is not available.
Acute toxicity:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; (Oral) Category 4⑦Content=0.015% No information available7) o-Phenylenediamine dihydrochloride; No data available8) Sulfuric acid (inhalation, rat); 4h LC50=347ppm(Oral, rat) LD50=2140mg/kg
Acute toxicity (Oral) Category 5
Acute toxicity (Inhalation; Dusts and mists) Category 2⑪Content=9.69% Acute toxicity (Inhalation; Dusts and mists) Category 310) Tween 20 (oral, rat); LD50=37000mg/kg
Inhalation (rat); >5.1mg/L, 4h
Skin corrosion/irritation:

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SDS No.000７0-011
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6/124), 9) EDTA2Na; No data available
6) Hydrogen peroxide (skin); Category 1⑦Content=0.015% No information available7) o-Phenylenediamine dihydrochloride; No data available8) Sulfuric acid; Category 1⑪Content=9.69% Category 110) Tween 20; No information available
Serious eye damage/irritation:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; Category 1⑦Content=0.015% No information available7) o-Phenylenediamine dihydrochloride; No data available8) Sulfuric acid; Category 1⑪Content=9.69% Category 110) Tween 20; No information available
Respiratory or skin sensitization:
Respiratory sensitization:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; Not classified
7) o-Phenylenediamine dihydrochloride; Classification not possible8) Sulfuric acid; Classification not possible10) Tween 20; No information available
Skin sensitization:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; Not classified
7) o-Phenylenediamine dihydrochloride; Category 18) Sulfuric acid; Not classified
10) Tween 20; No information available
Germ cell mutagenicity:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; Not classified
7) o-Phenylenediamine dihydrochloride; Category 28) Sulfuric acid; Classification not possible10) Tween 20; No information available
Carcinogenicity:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; Category 2⑦Content=0.015% No information available7) o-phenylenediamine dihydrochloride; Category 1B8) Sulfuric acid; Occupational exposure to Mist of inorganic strong acids
including sulfuric acid is classified to group 1 in IARC (to have

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10)
Reproductive toxicity:4), 9)
6)
7)
8)
10)

carcinogenicity for human ), group A2 in ACGIH (suspected
human carcinogens) and group K in NTP (known to havecarcinogenicity for human). With respect for the evaluation by
IARC and current evaluation by NTP, it should be classified tocategory 1, however since sulfuric acid itself is classified toCategory 4 in DFGOT and is not classified to carcinogen by anyother organization,
Classification not possible
Tween 20; No information available

EDTA2Na; No data available
Hydrogen peroxide; Classification not possible
o-Phenylenediamine dihydrochloride; No data available
Sulfuric acid; Not classified
Tween 20; No information available

Specific target organ systemic toxicity/Single exposure:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; Category 1⑦Content=0.015% No information available7) o-Phenylenediamine dihydrochloride;
Category 1 (Blood system)
Category 2 (Central nervous system)
Category 3 (Respiration tract irritation)
8)
10)

Sulfuric acid; Category 1 (Respiratory system)⑪Content=9.69% Category 2
Tween 20; No information available

Specific target organ systemic toxicity/Repeated exposure:4), 9) EDTA2Na; No data available
6) Hydrogen peroxide; Category 1 (Respiratory organs)⑦Content=0.015% No information available7) o-Phenylenediamine dihydrochloride; No data available8) Sulfuric acid; Category 1 (Respiratory system)⑪Content=9.69% Category 210) Tween 20; No information available
Aspiration hazard:4), 9)
6)
7)
8)
10)

EDTA2Na; No data available
Hydrogen peroxide; Classification not possible
o-Phenylenediamine dihydrochloride; No data available
Sulfuric acid; Classification not possible
Tween 20; No information available

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12． ECOLOGICAL INFORMATION
Information as the mixture is not available.
Aquatic environmental toxicity/Acute phase:4),9) EDTA2Na;
Scenedesmus quadricauda 72h EC50=1.01mg/L
6) Hydrogen peroxide; Category 1
Algae/aquatic plants ; 72h EC50= Nitzschia sp. 0.85mg/L
Oncorhynchus mykiss; 96h LC50=10.0-32.0 mg/L
Daphnia magna; 48h EC50=18-32 mg/L
⑦Content=0.015% No information available7) o-Phenylenediamine dihydrochloride; No information available8) Sulfuric acid; 96h LC50=16-28mg/L for fish (Lepomis macrochirus)
Category 3⑪Content=9.69% Not classified
10) Tween 20; No information available
Aquatic environmental toxicity/Chronical phase:4), 9)
6)
7)
8)

EDTA2NA; No data available
Hydrogen peroxide; Not classified
o-Phenylenediamine dihydrochloride; No data available
Sulfuric acid; 24h LC50=29mg/L for crustacea (Daphnia magna)
Category 1⑪Content=9.69% Not classified
10) Tween 20; No information available

Persistence and degradability:4), 9)EDTA2NA; No data available
6) Hydrogen peroxide; No information available7) o-Phenylenediamine dihydrochloride; Degree of decomposition, 0% by BOD(METI Existing chemical safety inspections)8) Sulfuric acid; No information available10) Tween 20; No information available
Bioaccumulative potential:4), 9) EDTA2NA; No data available
6) Hydrogen peroxide; No information available7) o-Phenylenediamine dihydrochloride; No information available8) Sulfuric acid; No information available10) Tween 20; No information available
Mobility in soil:4), 9) EDTA2NA; No data available
6) Hydrogen peroxide; No information available7) o-Phenylenediamine dihydrochloride; No information available

YK070 Human Chromogranin A EIAYANAIHARA INSTITUTE, INC.
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8)
10)

Sulfuric acid; No information available
Tween 20; No information available

Hazard to the ozone layer:4), 9) EDTA2NA; No data available
6) Hydrogen peroxide; No information available7) o-Phenylenediamine dihydrochloride; No information available8) Sulfuric acid; No information available10) Tween 20; No information available
13． DISPOSAL CONSIDERATIONS
Dispose of all waste material including containers in accordance with all applicable laws and local
environmental regulations.

YK070 Human Chromogranin A EIAYANAIHARA INSTITUTE, INC.
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14． TRANSPORT INFORMATION
ADR/RID

Sulfuric acido-Phenylenediamine ・2HCl

UN number

UN2796

UN3077
Environmentally hazardous substance,solid, n.o.s. (o-Phenylenediamine ・2HCl )
9III
Yes

Proper shipping name;Sulfuric acid

UN classification
Packing group
Marine pollutant

8IINot applicable

IMDG
UN number

Sulfuric acid
UN2796

Proper shipping name;Sulfuric acid

UN classification
Packing group
Marine pollutant

8IINot applicableo-Phenylenediamine ・2HCl
UN3077
Environmentally hazardous substance,solid, n.o.s. (o-Phenylenediamine ・2HCl )9III
Yes

No information available

No information available

IATA
UN number

Sulfuric acid
UN2796

Proper shipping name;Sulfuric acid8IIo-Phenylenediamine ・2HCl
UN3077
Environmentally hazardous substance,solid, n.o.s. (o-Phenylenediamine ・2HCl )
9III

Not applicable

Yes

Transport in bulk according toAnnex II of MARPOL 73/78 andthe IBC CodeUN classification
Packing group
Environmentally Hazardous
Substance

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15． REGULATORY INFORMATIONInternational Inventories
EINECS/ELINCS
TSCA⑪Listed⑪ListedJapanese regulations
Fire Service Act;Not applicable

Poisonous and Deleterious Substances Control Law;

Not applicable

Industrial Safety and Health Act;

⑩Substances with Health Hazards Prevention Guideline(Carcinogenicity Substance)⑪Group 3 Specified Chemical Substance, (Ordinanceon Prevention of Hazards Due to Specified Chemical
Substances Art.2 Para.1, Item 6)⑪Harmful Substances Whose Names Are to be
Indicated on the Label (Law Art.57, Para.1,
Enforcement Order Art.18)⑪Notifiable Substances (Law Art.57-2, Enforcement
Oder Art.18-2 Attached Table 9) No.613
Not applicable

Act on the Evaluation of Chemical Substances andRegulation of Their Manufacture, etc.;
Regulations for the carriage and storage of
dangerous goods in ship;

Civil Aeronautics Law;

Air Pollution Control Law;
Marine Pollution Prevention Law;
Act on Confirmation, etc. of Release Amounts ofSpecific Chemical Substances in the Environment
and Promotion of Improvements to theManagement Thereof (Pollutant Release andTransfer Register Law/ PRTR);
Water Pollution Control Act;
Export Trade Control Order;⑩Noxious Substances(Ordinance Art.3, Ministry ofTransportation Ordinance Regarding Transport by Shipand Storage, Attached Table 1)⑪Corrosive Substances(Ordinance Art.3, Ministry ofTransportation Ordinance Regarding Transport by Shipand Storage, Attached Table 1)⑩Miscellaneous Dangerous Substances and Articles(Ordinance Art.194, MITL Notification for Air
Transportation of Explosives etc. , Attached Table 1)⑪Corrosive Substances(Ordinance Art.194, MITL
Notification for Air Transportation of Explosives etc. ,Attached Table 1)⑪Specified substance⑪Enforcement ordinance Appendix No. 1 Noxious
liquid substance Category Y⑤Class I Designated Chemical Substances, No. 595⑪Specified substances(Law Art.2 Para.4, Enforcement
Order Art.3-3)
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16. OTHER INFORMATION
Reference and abbreviation
1) Internal data of Yanaihara Institute, Inc.
2) SIDS（2001）
3) RTECS ; Registry of Toxic Effects of Chemical Substances.
4) NTP DB (Access on Dec., 2005), National Toxicology Program5) SDS by FUJI FILM Wako Pure Chemical Corporation
6) ACGIH(2004); American Conference of Governmental Industrial Hygienists7) JSOH ; Japanese Society of Occupational Health
Recommendation of Occupational Exposure Limits (2021-2022)
8) NIOSH; National Institute of Occupational Safety and Health9) MSHA; Mine Safety and Health Administration
10) IARC(1992); International Agency for Research on Cancer
11) DFGOT; Occupational Toxicants; Critical Data Evaluation for MAK Value and Classification ofCarcinogens, Vol. 15, 2001
12) SDS by Dojindo Laboratories
13) SDS by Bio-Rad laboratories, Life Science GroupKey literature references and sources for data etc.;
NITE; National Institute of Technology and Evaluation (JAPAN) http;//www.safe.nite.go.jp/japan/db.html
IATA dangerous Goods Regulations, RTECS; Registry of Toxic Effects of Chemical Substances, Japan
Industrial Safety and Health Association GHS Model SDS, Dictionary of Synthetic Organic Chemistry,
SSOCJ, Koudansha Scientific Co. Ltd. , Chemical Dictionary, Kyouritsu Publishing Co., Ltd. etc.
The above information is believed to be correct to be the best of our knowledge and information,
But does not purport to be all inclusive and should be used as only a guide. This product is intended
to be used by expert persons having chemical knowledge and skill, at their own discretion and risk.
Yanaihara institute shall not be held liable for any damages resulting from handling or contact withthe above product. Users should determine the suitability of the information for their particularpurpose.