EN

Solutions for your Life Science lab

Solutions for your Life Science lab
Life Science has experienced a great growth all around the world in the past decade.
Scientists have made one breakthrough after another exploring the mysteries ofnature. Studying the most basic mechanisms of life in organisms can be extended totreat diseases, to give more possibilities and new hope to patients. Those benefits
for our world give a great meaning and depth to the field of Life Science, including
all biological sciences exploring the structures of life.

Solutionsfor yourLife Sciencelab

As the research gets deeper, the importance of sample preparations is rising, having
a great impact on the results. Repeatable and reproducible conditions and traceable
error sources are challenges that have to be tackled within continuously changing
and optimizing methods. The only constant in this varying system is the spirit ofscientists and tool makers to improve, as the results can only be as good as theequipment. The high-quality laboratory equipment of IKA has been valued for a longtime and its variety of products has become irreplaceable for biological, diagnostic
and medical laboratories.
Over the years IKA has developed its own distinctive product line, providing
solutions for your Life Science lab.
For more devices, accessories and information visit our website at www.ika.com.

PCR Sample Preparation

Molecular Cloning

Cell Culture

Page 4

Page 10

Page 16Microbial Culture

Immunoprecipitation

Western Blot

Page 20

Page 24

Page 28

In-situ Hybridization

Dialysis

Blood Tests

Page 32

Page 36

Page 38

4

PCR Sample Preparation

PCR Sample Preparation

Challenge 1
Animal and plant tissues need tobe disrupted at a low temperature to stabilize nucleic acids.

IKA’s solution
Tube Mill control
The intermittent running mode helps to improve sample

PCR Sample Preparation
PCR, or polymerase chain reaction, is a technique to amplify
specific DNA sequences. The key to a qualitative and successful
PCR is to minimize sources of error in the sample preparation
process.

size uniformity and can be accompanied by dry ice,
which avoids the thermal breakdown of DNA during highspeed grinding. The main unit has a number of programs

Tube Mill control

installed, which can be altered by the user and several

Ident. No. 0004180000

precautions for safe operations. Contamination-free processing can be achieved using single-use disposable millingchambers or autoclavable, reusable tubes.

Technical Data

Tube Mill control

Motor rating input / output

100 / 80 W

Speed range

5000 – 25 000 rpm

Useable volume max.

40 ml

Timer

5 s – 3 min

Circumferential speed max.

65 m/sFeed hardness max.

5 MohsFeed grain size max.

10 mm

Dimensions (W x H x D)

180 × 170 × 300 mm

5

6

PCR Sample Preparation

PCR Sample Preparation

Challenge 2
Cell lysis with enzymes like Proteinase K requires temperatureand time controlled incubation.

IKA’s solution
MATRIX thermoshaker
A wide range of interchangeable attachments enables
the thermoshakers to be flexibly adapted to different requirements and applications. Sample vessels with different
volumes as well as microtiter plates and deep well plates

ULTRA-TURRAX® Tube Drive P control
Ident. No. 0025005981*

T 10 basic
ULTRA-TURRAX®

can be mixed and tempered. Optionally, the insulated

Ident. No. 0003737000*

temperature gradients in the vessel during incubation.

and heatable lid “ThermoCover” can be added to prevent* T ubes are not included in the scope of delivery and haveto be ordered separately.

*D ispersing tools are not included in the scope of delivery.

ULTRA-TURRAX® Tube Drive P control

T 10 basic ULTRA-TURRAX®

The forward and reverse function can be used to help unwind

Compact, hand-held tissue homogenizer for volumes from 0.5

tangled fibres when the sample is fiber-rich. The multi-

to 100 ml with a quick-connect coupling for easy interchangea-

pur­pose device can be combined with tubes for different

bility of dispersing tools, and a wide range of plastic disposable

applications and is commonly used for tissue dispersion or celltools to avoid cross-contamination. Ideal for dispersing samples

disruption in plants and animals. There are disperser tubes,

in very small volume vessels, such as Eppendorf tubes or Greinerball mill tubes, stirring tubes or dissolver tubes available. It is

tubes.

equipped with a number of pre-stored programmes and safety precautions. Sterile-free disposable consumables are alsoavailable for high-throughput sample preparation.
Technical Data

T 10 basic
ULTRA-TURRAX®

Motor rating input / output

125 / 75 W

Volume range (H₂O)

0.5 – 100 ml

Technical DataULTRA-TURRAX® Tube
Drive P control

Motor rating input / output

36 / 28 W

Viscosity max.

5000 mPas

Speed range

400 – 8000 rpm

Speed range

8000 – 30 000 rpm

Display

OLED

Speed display

scale

Timer

10 s – 30 min

Speed control

stepless

Dimensions (W × H × D)

122 × 54 × 178 mm

Noise without element

65 dB(A)

Weight

1.3 kgExtension arm diameter

8 mm

Extension arm length

100 mmWeight0.61 kg

Dimensions (W × H × D)

56 × 178 × 66 mm

Protection class according to
DIN EN 60529

IP 20

Protection class according to
DIN EN 60529

IP 30

Technical Data

MATRIX Orbital MATRIX Orbital MATRIX Orbital MATRIX Orbital
MATRIX Orbital Delta F0.5
Delta F1.5
Delta F2.0
Delta FP

MATRIX Delta
Plus

MATRIX Orbital
Delta Plus

Ident. No.

0010006246

0010006255

0010006249

0010006258

0010006252

0010006850

0010006853

Mixing function

√

√

√

√

√

×

√

Heating function

×

√

√

√

√

√

√

Cooling function

×

×

×

×

×

√

√

Attachments

variable

0.5 ml tubes

1.5 ml tubes

2.0 ml tubes

plates(MTP/DWP)

variablevariable7

8

PCR Sample Preparation

PCR Sample Preparation

Challenge 3

IKA’s solution

To extract nucleic acids fromthe lysate, several purification
steps need to be performed.

IKA PETTE vario
An ergonomic feel combined with uncompromisingly
precise performance: the IKA PETTE vario can be used
for many applications. The colour coding simplifies the
rapid selection of a suitable pipette. The highly precise
pipettes are fully autoclavable and resistant to UV-lightand chemicals to ensure sterile working conditions androbust, long-lasting quality. Accidental volume changes
can be avoided with the volume locking feature to

IKA G-L

IKA mini G

ensure accurate pipetting results.

Ident. No. 0030000774*

Ident. No. 0003958000** The scope of delivery includes the metal rotor and cover for 12 vessels, with a capacityof 1.5 / 2 ml.

* The scope of delivery includes rotors for 1.5/ 2 ml tubes and 0.2 ml PCR strips as well as
several adapters for smaller tubes.

IKA G-L

IKA mini G

IKA G-L is the ideal midi centrifuge for all types of sepa-

Mini centrifuge for all applications which do not require

ration processes such as the extraction of nucleic acids

high speeds, like spinning down sample tubes, microfiltra-

or for simple spin down applications. It can be used with

tions or cell separation in tubes such as PCR vessels or PCR

standard 1.5 / 2 ml vessels, as well as with PCR strips.

strips. The transparent cover allows constant observation

The cover opens automatically at the press of a button.

of the centrifuge. As a safety feature, the centrifuge will

The centrifuge is made of quality and sturdy materials,

run only when the lid is closed.

ensuring that it remains stable even during high speed
operation. To save time, programs can be set for repeatedexperiments.

IKA PETTE vario

Ident. No.

Max. volume tip

Technical Data

IKA G-L

Technical Data

IKA mini G

Capacity

12 × 1.5 / 2.0 ml

Capacity

8 × 1.5 / 2.0 ml

0.1 – 2 μl

0020011210

10 μl

Centrifugal acceleration

16 500 G

Centrifugal acceleration

2000 G

0.5 – 10 μl

0020011211

10 μl

Speed range

800 – 15 700 rpm

Speed range

6000 rpm

2 – 20 μl

0020011213

200 μlMotor rating input

65 W

Motor rating input

12 W

10 – 100 μl

0020011214

200 μlUnbalance sensor

yes

Unbalance sensor

No

20 – 200 μl

0020011215

200 μlQuick stop

yes

Quick stopyes

100 – 1000 μl

0020011216

1000 μl

Cover opening

automatic

Cover opening

automatic

0.5 – 5 ml

0020011217

5 ml

Protective coveryesProtective coveryes

1 – 10 ml

0020011218

10 ml

Dimensions (W × H × D)

200 × 140 × 280 mm

Dimensions (W × H × D)

155 × 105 × 175 mm

Weight3.9 kgWeight

1.4 kgInformation on fixed-range pipettes and suitable tips can be found on
www.ika.com.

9

10

Molecular Cloning

Molecular Cloning

Challenge 1
The extraction of nucleic acids
is crucial for the success of thewhole experiment. Therefore thesample has to be disrupted carefully and under low temperature
conditions to prevent degradation of the DNA material.

IKA’s solution
Molecular Cloning
Molecular cloning is a method to produce many
copies of a specific DNA fragment. The DNA or RNA
is extracted from a sample and amplified in a PCR.
The amplified fragment can then be digested withrestriction enzymes and ligated with a vector to
produce a plasmid with the desired DNA. A microorganism such as E.coli is then transformed with theplasmid, enriched and harvested to produce a highquantity of plasmids containing the desired DNA
fragment.

Tube Mill control

ULTRA-TURRAX® Tube Drive P control

The sample can be mixed with dry ice directly during

The disperser ensures the best stirring, dispersing,processing to avoid thermal degradation of DNA dur-

grinding and homogenization results and has been

ing high speed grinding (see page 5 for details).

designed in particular for the safe processing of
infectious, toxic or odour-intensive samples. With theforward and reverse function, the efficiency of homogenization is increased and tangled fibers can be easily
unwound (see page 6 for details).

T 10 basic ULTRA-TURRAX®
Compact, hand-held tissue homogenizer for volumes
from 0.5 to 100 ml. With its small diameter, samples
in small tubes can be easily dispersed, without theneed to transfer the samples to another vessel, hence
preventing sample loss during transfer (see page 6 fordetails).

11

12

Molecular Cloning

Molecular Cloning

Challenge 2
All enzyme reaction steps andthe cultivation of E.coli requireprecise control of temperatureand time to be able to successfully produce the plasmid.

IKA’s solution
MATRIX
Mixing, heating and cooling functions are performed
as required with precise temperature control. Uniform
tempering of the samples is ensured by the various
attachments fitting perfectly to the common reaction

Dry Block Heater

vessels in Life Science labs. The MATRIX thermoshakers

Digital dry bath incubator for aluminum blocks provides

are ideal for incubation of enzymatic reactions (seeprecise temperature control in small vessels. Included PT

page 7 for details).

1000.60 temperature sensor allows temperature control
directly in the sample vessel. It can be used with PCR
tubes, PCR strips, Greiner tubes, microplates and cuvettes.

Dry Block Heater 1 | 2 | 3 | 4Technical Data

Dry Block Heater 1 | 2 | 3 | 4

Number of blocks

1|2|3|4

Heat output

165 | 250 | 330 | 412 W

Ident. No. 0004025300

Heating temperature range

RT +5 °C – 120 °C

Ident. No. 0004025400

DisplayLED

Adjustment and display
resolution±1K

Fixed safety circuit

150 °CPT 1000 variation;
DIN EN 60751 Kl. A≤ ± (0.15 + 0.002xITI) K

Set-up plate material

Aluminum alloy

Time setting range

1 min – 99 h 59 min

Ident. No. 0004025100
Ident. No. 0004025200

13

14

Molecular Cloning

Molecular Cloning

ICC control pro 20 c

KS 3000 i control | ic control

KS 4000 i control | ic control

Ident. No. 0008036400

Ident. No. 0003940000 | 0003940100

Ident. No. 0003510000 | 0003510100

ICC control pro 20 c

KS 3000, KS 4000

Water bath solution with powerful pressure / suction pump

The KS 3000 / 4000 incubator shakers provide stable fixing of multiple tubes, flasks orand external temperature sensor, perfect for precise tem-beakers in various sizes and shapes. Available in two sizes and two models. The con-pering of enzyme reaction samples in floating racks or tube

trol model offers more features, such as cooling down to -10 °C (when connected toracks. The bath is easy to clean, as there are no dead spaces

an external chiller). All models ensure reliable and unattended shaking and temperingand the vessel holders are easily exchangeable.

of samples or cultured organisms.

Technical Data

KS 3000 i | ic

KS 4000 i | ic

Type of movement

OrbitalOrbitalCompact immersion
circulator

Shaking stroke

20 mm

20 mm

Heat output (230 / 115 V)

2000 / 1000 W

Permissible shaking weight(incl. attachment)

7.5 kg

20 kg

Temperature range

RT +10 °C* – 150 °C

Motor rating input / output

45 / 10 W

82 / 24 W

Display

TFT

Permissible ON time

100 %

100 %

Temperature stability DIN 12876± 0.01 K

STICKMAX

Speed range

10 – 500 rpm

10 – 500 rpm

Max. flow rate

18 l/minFor flexible arrangement of various vessels

Max. pump pressure

0.3 barTemperature range (inlet T>3°C)

RT +5 °C – 80 °C |RT -10 °C – 80 °C

RT +5 °C – 80 °C |RT -10 °C – 80 °C

Min. suction pressure

0.2 barTime setting range

1 min – 999 h

1 min – 999 h

Dimensions (W × H × D)

145 × 340 × 200 mm

Timer display

LED

LED

Operating mode

Timer and continuous
operation

Timer and continuous
operation

Dimensions (W x H x D)

465 × 430 × 695 mm

580 × 525 × 750 mm

Technical Data

ICC control

Appliance typeon shakers or incubators. Easy to clean anddisinfect.
Ident. No. 0003920000* Possible operating temperatures with cooling coil: -20 °C to 150 °C

15

16

Cell Culture

Cell Culture

Challenge 1
To obtain primary cell lines, thetissue has to be disrupted sufficiently, but carefully, to ensure
cell viability.

Cell Culture

IKA’s solution

Cultivating cells is generally divided in cultivating
immortalized cell lines or primary cells. The former
can be grown without limit, whereas the latter
have to be obtained freshly from the tissue and its
replication capacity is limited. If the cells are notused immediately, they can be frozen for storage
and thawed up when needed. The thawing process
must be controlled carefully to prevent damage tothe cells.

Dry Block Heater 3
Digital dry bath can be used in clean rooms, cell rooms,
etc. Safe and convenient working is guaranteed, as
they incubate without using water. This prevents contamination of bacteria (see page 13 for details).

T 10 basic ULTRA-TURRAX®
Suitable for mechanical tissue disruption. Due to its
small diameter, even tissue samples in small tubes
can be dispersed directly in the tubes easily. The
compact and hand-held design is especially madefor smaller volumes from 0.5 to 100 ml. The metallic dispersing tool is autoclavable to ensure sterile
dispersing (see page 6 for details).

17

18

Cell Culture

Cell Culture

Challenge 2

Challenge 3

Cells must be thawed up
evenly to ensure their viability.
Media or additives have to be
prewarmed.

Incubation steps occur overlonger time periods, requireprecise temperature control
and often sterile conditons.

IKA’s solution

IKA’s solution

ICC control pro 20 c

MATRIX Orbital Delta Plus

ROLLER 10 digital

KS 4000 ic control

The different attachable racks and floating tube racks

This thermoshaker ensures precise mixing and tem-

The digital roller shaker with 10 rolls provides smooth

Incubator shaker for reliable and unattended culti-

make the water bath compatible for centrifuge tubes

pering. The different modes for slow or fast heatingrocking and rolling action at infinitely adjustable speeds. It

vation of cells. Ideal for shaking and tempering offrom 0.5 ml to 50 ml. Suitable accessories facilitate also

and the specific 1.5 / 2 ml centrifuge tube attachment,

is ideal for rotating cell cultures in conventional tubes andmedia and cell cultures in different vessels, such as

the tempering of larger media bottles or beakers (seeallow the controlled thawing of the cells at specificcylindrical flasks of various sizes cultivated in an incubator

flasks, beakers, plates or smaller tubes. The beakers

page 14 for details).

rates (see page 7 for details).

with up to 50 °C. Easily removable attachments provide

and flasks can be fixed with clamps. Plates or smaller

for quick cleaning in case of sample spills.

tubes for cell cultures can be safely attached with theadhesive mat STICKMAX (see page 15 for details).

ROLLER 10 digital
Ident. No. 0004013000

Technical Data

ROLLER 10 digital

Type of movement

Rocking and rolling

Speed range

0 – 80 rpm

Timer display

7 segment LED

Speed display

7 segment LED

Operating mode

Timer and continuous
operation

able handles make the pipette easy and com-

Number of rolls

10

and robust, using high quality UV resistant andDimensions (W × H × D)

380 × 115 × 545 mm

chemical resistant materials. The pipettes are fully

Permissible ambient temperature4 – 50 °C

Protection class according to
DIN EN 60529

IP 21

IKA PETTE
Single channel pipettes with different sizes ranging
from 0.1 μl to 10 ml. The design and interchangefortable to operate. The construction is durable

autoclavable to ensure sterility and complianceto stringent laboratory standards, which makes it
ideal for the use in cell culture applications (seepage 8 for details).

19

20

Microbial Culture

Microbial Culture

Challenge 1
The various media and its additives require extensive preparation, like weighing multiple
components, adjusting the pH
and need heated stirring steps.

IKA’s solution
RET control-visc
Magnetic stirrer with heating and integrated balance. To
facilitate the preparation of buffer solutions, a BNC socket
allows the connection of a pH electrode. As pH is an important parameter for microbial growth, pH variations can lead to
microbial deactivation and inactivity. The integrated temperature control feature enables connection of a temperature
sensor, placed directly in the medium, to control the actual
temperature with a high degree of precision. Program editing

Microbial Culture

and storage functions allow customization of speed, time andtemperature for unattended mixing and tempering.

A high variety of viruses, fungi and bacteria are
cultivated in Life Science laboratories. In accordancewith their natural habitats, these microorganisms
require certain climates and conditions to growwell. When cultivated in a laboratory, special media
is needed in order to provide the necessary nutrientsfor them to thrive.

RET control-visc

Technical Data

RET control-visc

Ident. No. 0005020000*

Stirring quantity max. (H₂O)

20 lMotor rating output

9W

Speed range

50 – 1700 rpm

Heat output

600 W

Heating temperature range

RT – 340 °C

Adjustable safety circuit

50 – 380 °C

Load capacity weighing function
max.

5 kg

Set-up plate material

stainless steel 1.4301

Set-up plate dimensions

Ø 135 mm

Dimensions (W × H × D)

160 × 85 × 270 mm

Weight2.7 kg* A PT 100.70 temperature sensor is suppliedwith the unit.

21

22

Microbial Culture

Microbial Culture

Challenge 2

Challenge 3

Thawing or heating of media
and components before usage.

Cultivation of microorganisms
needs sterile conditions to
avoid contamination.

IKA’s solution

IKA’s solution

ICC control pro 20 c

MATRIX Orbital Delta Plus

KS 3000, KS 4000

IKA PETTE

The variety of hookable racks and floating racks give

This thermoshaker ensures precise mixing and tem-

The KS incubator shakers are ideal for the shaking

Single channel pipettes with different sizes ranging

the waterbath many purposes from prewarming me-

pering. The different modes for slow or fast heating

and tempering of media and cell cultures in different

from 0.1 μl to 10 ml. All pipettes and components

dia, cultivating cells to tempering reaction mixes. Withand the specific 1.5 / 2 ml centrifuge tube attachment,

vessels, such as flasks, beakers, plates or smaller tubes.

are fully autoclavable and UV resistant for working ina bottom plate, even bigger bottles or beakers can beallow the controlled thawing of the cells at specific

The beakers and flasks can be fixed with clamps. Plates

sterile or decontaminated conditions (see page 8 forplaced easily in the bath (see page 14 for details).

rates (see page 7 for details).

or smaller tubes for cell cultures can be safely attacheddetails).

with the adhesive mat STICKMAX (see page 15 fordetails).

STICKMAX
For added safety and flexible loading, the adhesive mat
STICKMAX easily keeps vessels in place quickly and securely.

23

24

Immunoprecipitation

Immunoprecipitation

Challenge 1
The sample has to be incubatedovernight and slowly agitated.

Immunoprecipitation
Immunoprecipitation is a method of selectively purifying
and enriching a protein. Through a specific antibody-protein reaction, the target protein is isolated from a sample
containing thousands of different proteins, then enrichedand purified.

IKA’s solution
Trayster digital

Trayster digital

Digital overhead shaker with vertical rotating motion for

Ident. No. 0004006000*

smooth but effective 360 degree mixing with infinitely
adjustable speed. Ideal for biological samples, particularly
blood samples. The variety of tube insert sizes allow sample mixing in Eppendorf tubes and Greiner tubes up to 50
ml. Perfect for immunoprecipitation as it can be timed andplaced in the refrigerator or incubator.

Technical Data

Trayster digital

Type of movement

Overhead

Permissible shaking weight(incl. attachment)

2 kg

Motor rating input / output

16 / 9 W

Speed range

0 – 80 rpm

Speed display

7 segment LED

Time setting

1 s – 99.9 h

Timer display

7 segment LED

Dimensions (W × H × D)

460 × 180 × 140 mm

Weight3.282 kg

Permissible ambient temperature4 – 50 °C* Attachements are not included in the scopeof delivery, please order separately.

25

26

Immunoprecipitation

Immunoprecipitation

Challenge 2

Loopster digital

The tissue sample has to belysed fully, but not excessively
to safely release the target
protein and contamination has
to be avoided.

Ident. No. 0004016000**A ccessories, such as tube attachments,
have to be ordered separately.

IKA’s solution
Loopster digital

KS 4000 ic control

IKA PETTE

T 10 basic ULTRA-TURRAX®

MATRIX

Digital rotator for smooth but effective mixing with adjust-The temperature control mode ensures that the set

Single channel pipettes with different

Compact, hand-held tissue homogeniser

The perfect assistant for incubating

able speed. Ideal for biological samples and suitable fortemperature remains accurate even after long hours ofmodels ranging from 0.1 μl to 10 ml.

for small volumes can be combined withsamples in the laboratory. With a wide

separating and mixing processes of powdered and liquid

operation. Features such as the antibacterial coating

They can be used for a wide range ofa wide range of plastic disposable heads

range of functions and programmablesamples in Eppendorf tubes and Greiner tubes up to 50 ml.

of the controls, the liquid pressure struts (to assist andlaboratory applications, as they are highly

to avoid cross contamination (see page 6

temperature and mixing series, theThe easy removable attachments allow simple and quick

prevent the hood from falling), the emergency stop forchemical resistant, fully autoclavable andfor details).

MATRIX shakers perfectly meet thecleaning of the unit. The universal plate system allows a

opening the hood and the sump drainage are all parthighly precise. Simple liquid transfer anddiverse needs of enzymatic or chemical

flexible and personalized attachment of individual clips in allof a design based on safety and user-friendliness. The

handling of microliter volumes can bereaction conditions (see page 7 forsizes. Ideal for usage in a refrigerator or incubator.

standard USB interface allows remote control and un-done easily, accurately and precisely (seedetails).

interrupted recording of experiment-related parameters

page 8 for details).

(see page 15 for details).

Technical Data

Loopster digital

Type of movement

Rotating

Permissible shaking weight(incl. attachment)

2 kg

Motor rating input / output

16 / 9 W

Speed range

0 – 80 rpm

Speed display

7 segment LED

Timer display

7 segment LED

Operating mode

Timer and continuous
operation

Dimensions (W × H × D)

300 × 360 × 300 mm

Permissible ambient temperature4 – 50 °C27

28

Western Blot

Western Blot

Challenge 1
Gentle disruption and lysis of
the sample is needed, often invery small sample volumes.

IKA’s solution
VORTEX 3
Practical vortex shaker with a sturdy construction and stable

Western Blot

operation - touch or continuous. The device is suitable forcentrifuge tubes, conical flasks, enzyme plates etc. due tothe availability of a high variety of attachments. For safe,

In Western blotting, cells or biological tissue samples are prepared for separation via gel electrophoresis, transferred to a membrane and then stainedwith antibodies bound to a specific protein. The
location and intensity of the antibody staining isanalyzed to obtain information about the expression
level and size of the proteins in the sample.

reliable and hands-free operation.

Vortex 3
Ident. No. 0003340000

Technical Data

VORTEX 3

Type of movement

orbital

Shaking stroke

4 mm

Permissible shaking weight(incl. attachment)

0.4 kgMotor rating input / output

58 / 10 W

Speed range

500 – 2500 rpm

Speed control

scale (0 – 6)

Timer displaynoOperating mode

continuous operation

Dimensions (W × H × D)

127 × 136 × 149 mm

Weight4.5 kg29

30

Western Blot

Western Blot

Challenge 2
Proper staining and destainingis achieved with long incubation times and gentle shaking.

IKA’s solution
T 10 basic ULTRA-TURRAX®

Dry Block Heater 1

ROCKER 3D digital

Compact, hand-held tissue homogeniser for volumes

Digital dry bath can be used in clean rooms, cell rooms,

Digital shaker with three-dimensional tumbling motion and

ROCKER 3D digitalfrom 0.5 to 100 ml, a quick-insert interface for easy

etc. Safe and convenient working is guaranteed, as

adjustable speed for smooth mixing tasks. Ideal for gentle

Ident. No. 0004001000

interchangeability of dispersing tools, and a wide range

they incubate without using water. This prevents con-incubation at low speeds and for continuous operationof disposable plastic tools to avoid cross contamination

tamination of bacteria (see page 13 for details).

modes. Various clamps and the STICKMAX are available(see page 6 for details).

for the attachment of different kind of vessels such as
flasks, tubes or plates. The device can also be placed in an
incubator for temperatures up to 50 °C.

Technical Data

Rocker 3D digital

Type of movement

Tumbling

Tumbling angle adjustable0 – 15°Permissible shaking weight(incl. attachment)

2 kg

Motor rating input / output

16 / 9 W

Permissible ON time

100 %Speed range

0 – 80 rpm

Speed display

7 segment LED

Time setting

1 s – 99.9 h

Timer display

7 segment LED

Operating mode

Timer and continuous
operation

Dimensions (W × H × D)

280 × 185 × 330 mm

Weight2.3 kgPermissible ambient temperature4 – 50 °C

Permissible relative humidity

80 %IKA PETTE
Single channel pipettes with different models ranging
from 0.1 μl to 10 ml (see page 8 for details).

31

32

In-situ Hybridization

In-situ Hybridization

Challenge 1
For the enzymatic digestion,
precise control of temperatureis required and contamination
has to be avoided.

In-situ Hybridization
In-situ hybridization is used to detect specific nucleic acids
in cells or tissue samples. Labelled DNA or RNA is inserted
into the cells, where they anneal to complementary nucleic
acids Depending on the type of nucleic acids used in theprobe and sample, the hybridization process can be divided into three types: DNA-DNA hybridization, DNA-RNA
hybridization and RNA-RNA hybridization.

IKA’s solution
Dry Block Heater 3

ICC control pro 20 c

MATRIXDry bath incubator used with PCR tubes,

A wide range of accessories is available

The easily exchangeable attachments and

PCR strips, Greiner tubes, microplates andto match the requirements of incubation

the intuitive interfaces of the thermoshak-cuvettes (see page 13 for details).

reactions such as enzymatic digestion. Theer facilitate every day steps in the labora-

bath is easy to clean with no dead spaces

tory. Enzyme reaction conditions can be

and therefore helps to reduce contamina-

set and even automatically stopped withtion risks (see page 14 for details).

a programmed increase of temperature
after a defined time period (see page 7
for details).

33

34

In-situ Hybridization

In-situ Hybridization

Challenge 2
The effect of hybridization
depends on the degree ofcoverage of the sample withthe reaction solution, as well asa sufficient incubation.

IKA’s solution
KS 260 basic

ROCKER 3D digital

IKA PETTE

Made for smooth agitation of cell cultures, nutrient media in Petri

Made for smooth agitation of cell cultures, nutrient

Highly precise single channel pipettes with different

dishes, culture bottles and vessels in an orbital shaking motion.

media in Petri dishes, culture bottles and vessels in anmodels ranging from 0.1 μl to 10 ml. They can be used

orbital shaking motion (see page 31 for details).

for a wide range of laboratory applications, as they are

Comes with an integrated slip resistant foil (PP) which prevents

KS 260 basic

vessels from sliding off during long operation times. Can run for a

Ident. No. 0002980200

highly chemical resistant, fully autoclavable and ergo-long time period at a low speed level to promote an even coverage

nomic in their design with exchangable handles to fit

of membranes during incubation. The LED display allows real-time

perfectly into any user’s hand. The risk of RSI is greatly

observation of speed and time during the mixing process. For moreminimized with the ergonomic design of the IKA PETTE

advanced experimental settings, the KS 260 control model is availa-(see page 8 for details).

ble with additional features.

Technical Data

KS 260 basic

Type of movement

orbital

Shaking stroke

10 mm

Permissible shaking weight(incl. attachment)

7.5 kgMotor rating input / output

45 / 10 W

Speed range

0 – 500 rpm

Speed display

LED

Time setting range

5 – 50 min

Operating mode

timer and continuous
operation

Dimensions (W × H × D)

360 × 98 × 420 mm

Weight8.5 kg35

36

Dialysis

Dialysis

Dialysis
Dialysis is a method to physically separate and purify
biological macromolecules from small molecules byusing a semi-permeable membrane. Small molecules
diffuse out of the membrane into the surrounding
water or buffer solution and the macromolecules remaining in the membrane can be further processed.

Challenge 1

Challenge 2

Stable and effective stirring
is required without constantobservation of devices.

If many vessels are usedsimultaneously, space needs tobe saved and multiple stirring
stations are needed.

IKA’s solution

IKA’s solution

big squid white

RO 5

Digital LED display showing the speed and outstanding

Noiseless and consistent stirring on 5-positions withoutchemical resistance due to the glass top and the synthetic

heating. The RO 5 is designed for synchronous stirringbottom made of TPC-ET. The big squid is long-lasting andand therefore ideal for dialysis set-ups with more than one

reliable for dialysis experiments, providing a means forsample. The models RO 10 and RO 15 provide you withgentle, low shear mixing of low viscosity liquids.

even more mixing points.

big squid white

RO 5

Ident. No. 0003672000

Ident. No. 0003690500

Technical Data

big squid white

Stirring quantity max. per stirring position (H₂O)

1.5 l

Motor rating input / output

4/2W

Speed range

0 – 2500 rpm

Speed display

LED

Stirring bar length max.

30 mm

Setting accuracy speed

50 rpm

Set-up plate materialglass

Set-up plate dimensions

Ø 160 mmTechnical Data

RO 5

Number of stirring positions

5

Stirring quantity max. (H₂O)

0.4 l

Stirring position distance

90 mm

Speed display

LED

Speed range

0 – 1200 rpm

Setting accuracy speed

10 rpm

Set-up plate material

stainless steel 1.4301

Set-up plate dimensions

120 × 470 mm

37

38

Blood Test

Blood Test

Challenge 1
After obtaining a blood sampleof the patients, it has to bequickly processed to prevent
coagulation.

IKA’s solution
Blood Test
By analyzing human blood, diseases like anaemia,
diabetes, blood diseases, microbial infections, kidney or liver dysfunctions and physical abnormalities
can be diagnosed. Levels of cholesterol, calcium andmore can also be tested. Blood tests are an integral
part of many medical treatments in hospitals or
blood donation centers.

ROLLER 6 digital

Dry Block Heater 4

The digital roller shaker with 6 rolls provides smooth

Digital dry bath can be used in cell culture rooms,

rocking and rolling action for rotating all kind of

biosafety cabinets, laminar flow cabinets etc. The

common blood collection tubes. No additional clamps

exchangeable blocks are available for various vessel

are needed to fasten the bags or tubes. The device candesigns and as they incubate without using water,

also be placed in a refrigerator or incubator with tem-the risk of contaminating the blood samples is greatly

peratures ranging from 4 - 50 °C. In case of spillage

reduced. The device is supplied with a PT 1000.60

and for easy cleaning and disinfection, the rolls can bestainless steel temperature sensor which can be directly

removed easily (see page 19 for details).

inserted into the sample vessel for monitoring the actual sample temperature throughout the entire process(see page 13 for details).

IKA PETTEROCKER 3D digital

Single channel pipettes with different models ranging

Digital shaker tumbling motion can be used for mixing

from 0.1 μl to 10 ml. Pipettes can be used in blood

blood bags to prevent the blood from coagulating (seedonation centers to identify patient blood types on-site

page 31 for details).

with a simple prick of a fingertip (see page 8 for details).

MATRIX thermoshaker

Trayster digital

For more advanced temperature and mixing processes

Digital overhead shaker, ideal for mixing biologicalwith additional requirements (see page 7 for details).

samples, particularly blood sam­ples (see page 25 fordetails).

39

EN

GERMANY
IKA-Werke GmbH & Co. KG
Janke & Kunkel-Straße 10, 79219 Staufen
Phone: +49 7633 831-0, eMail: sales@ika.de

USA
IKA Works, Inc.
Phone: +1 910 452-7059
eMail: sales@ika.net

KOREA
IKA Korea Ltd.
Phone: +82 2 2136 6800
eMail: info@ika.kr

BRAZILIKA Brazil
Phone: +55 19 3772-9600
eMail: sales@ika.net.br

MALAYSIA
IKA Works (Asia) Sdn Bhd
Phone: +60 3 6099-5666
eMail: sales.lab@ika.my

CHINA
IKA Works Guangzhou
Phone: +86 20 8222 6771
eMail: info@ika.cn

POLANDIKA Poland Sp. z o.o.
Phone: +48 22 201 99 79
eMail: sales.poland@ika.com

JAPAN
IKA Japan K.K.
Phone: +81 6 6730 6781
eMail: info_japan@ika.ne.jp

INDIA
IKA India Private Limited
Phone: +91 80 26253 900
eMail: info@ika.in

UNITED KINGDOMIKA England LTD.
Phone: +44 1865 986 162
eMail: sales.england@ika.com

VIETNAMIKA Vietnam Company Limited
Phone: +84 28 38202142
eMail: sales.lab-vietnam@ika.com

THAILANDIKA Works (Thailand) Co. Ltd.
Phone: +662 1178150
eMail: sales.lab-thailand@ika.com

TURKEYIKA Turkey A. Ş.
Phone: +90 216 584 54 65
eMail: info@ika.com

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Information regarding delivery is not binding.

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