Hinoki Cypress (Chamaecyparis Obtusa) Leaf Oil-Natural Essential Oil with Antibacterial activityThe essential oil is extracted via steam distillation process fromleaves of cypress trees grown in Kii Peninsula including Nara, Mie,and Wakayama Prefectures. The main component is Hinokitiol (or4-Isopropyltropolone with chemical formula of C10H12O2）that has
antibacterial property.
We use the aroma diffuser to diffuse the oil in this room so that you
can enjoy the refreshing fragrance of forest green, while inhibiting
bacterial and viruses.

（Hinoki cypress leaves have beenused for removal of evil karma in
Shugendo ritual fire ceremony.）（Hinoki cypress leaves have beenused for food preservation sinceancient times.）

Antibacterial / Antivirus performance
1) Hinokitiol with a concentration between 100 and 200 ppm (μg/ml) may inhibit the growth of
Salmonella typhi, Escherichia coli, Vibrio cholerae, Bacillus dysentericus, and Staphylococcus,
and the same with concentration of 50 ppm (μg/ml) may inhibit the growth of Corynebacterium
diphtheriae, according to the study by Dr. Kyohei Suetsugu. (Source: Journal of the Kumamoto
Medical Society 24)2) In dermatological treatment, Hinokitiol has nontoxic, excellent efficacy against fungus that causes
athlete's foot or tinea pedis, according to the study by Dr. Jiro Ikegami. (source: Journal of Niigata
Medical Society 68)3) Hinokitiol is characterized by its wide range of antibacterial spectrum (the range of different
bacterial and microorganisms that Hinokitiol inhibits or kills them) and the low level of minimum
inhibitory concentration (MIC-- the lowest concentration of Hinokitiol that will inhibit the visible
growth of a bacterium or a microorganism after overnight incubation). According to the study by
Dr. Toshihiro Okabe disclosed in Fragrance Journal 2 (1989), the antibacterial efficacy wasconfirmed with a concentration of 2.5 to 200μg/ml.

4) According to antibacterial activity test conducted with an automatic disinfectant robot that
diffuses Hinokitiol solution with the concentration of 250 ppm, antibacterial activity at apercentage of 95 to 100% was found against Staphylococcus aureus, MRSA, Escherichia coli, andPseudomonas aeruginosa. The robot was equipped with a diffusing nozzle with diameters of 10to 100 μ and diffuses the solution at the amount of 10 ml per 10 cm2.
5) With respect to antivirus efficacy of Hinokitiol, some patents (shown below) were applied and forthe past 20 years. Unfortunately, no information on the inhibitory effect to the novel coronavirus
have been reported so far (because any laboratory institutes have not captured the virus stain
and/or not yet developed safety measures to handle it, or probably because it is a confidential
military matter.)

However, we suppose through supportive data that Hinoki cypress leaf oil caninhibit the novel coronavirus. And we reportedly know that some groups from China have been
struggling for capturing Hinokitiol.

Maybe they can imagine the efficacy through healthyexperience in a forest or a mountain torrent filled with a lot of trees and negative ions. We willcontinue to research the evidence of the efficacy of Hinoki cypress leaf oil against the novel
coronavirus.
Patent information(1) JP 2005-68089 A: coronavirus disinfectant by Hinoki Clinical(2) JP 2009-173555 A: Protection spray against human influenza by JCS.
(3) JP 2009-167105 A: Protection spray against avian influenza by JCS.
(4) JP 2006−158361: livestock protection sheet against highly pathogenic avian influenza byImmunobacs Japan.