INSTRUCTIONSFOR USE
96 WELL MULTI-MAGPEN
Item No.: 657896
Your smart assistant for
3D cell culture transfer

MULTI-MAGPEN INSTRUCTION MANUAL
Thank you for purchasing this Greiner Bio-One product. The Multi-MagPen is used to transfer, collect,organize, or layer magnetic 3D cell cultures without disrupting their tissue architecture. 		
The Multi-MagPen uses a simple “pick-up-and-drop” method to reliably transfer 3D cell cultures ­between
microwell plates.
The magnets in this kit are strong, can damage electronics, and cause injury if not handled correctly.
Read the safety precautions below carefully to ensure correct handling of the magnets.

SAFETY PRECAUTIONS
To guarantee problem free and safe operation of the Multi-MagPen please read these safety precautions
before using.

///////The magnet plates contain strong neodymium magnets that must be handled with extremecare.
When storing magnets in proximity to other magnets or materials that are attracted to magnets, take precautions so that objects do not slam together. Neodymium magnets are brittle and can shatter or crack, sometimes producing dangerous fragments moving at highspeeds. Fingers can also be severely pinched between magnets or between magnets andcertain metals.
Keep the magnetic drives spatially separated and DO NOT put the drives together at anytime. Due to the magnetic force, placing them in close proximity can cause them to “crash”
together, resulting in damage to the drive magnets and/or structure.
Persons with pacemakers or similar medical devices should not come near Neodymium
magnets.
Neodymium magnets can damage magnetic media such as credit cards, magnetic ID cards,televisions, computer memory and computer monitors. Keep magnets at least 30 cm(12 inch.) from these devices away.
Neodymium magnets should not be burned or machined. They will lose their magnetic pro
perties if heated above 80 °C (175 °F). DO NOT AUTOCLAVE the magnetic drives.
Neodymium magnets are not toys. The magnetic drives should only be used for their intended purpose. Children should not be allowed to play with them.

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

IFU F076057 EN [rev.00, 07.2021]

1/ INTENDED USE
The Multi-MagPen is a general laboratory product for 3D cell culture to be used by qualified personnel
in a laboratory environment. It is intended for research use only and not approved for human or animaluse.

2/ MATERIALSMULTI-MAGPEN KIT & SLEEVE
Item No.

Product Description

Content/Packaging

657896

96 Well Multi-MagPen Kit

96 Well Multi-MagPen Drive, transparent, nonsterile, 1 pcs./box, 96 Well Multi-MagPen Sleeve,
PP, transparent, sterile, Cell Repellent,
1 pcs./bag, 2 pcs./box

651596

96 Well Multi-MagPen Sleeve

96 Well Multi-MagPen Sleeve, PP, transparent,Cell Repellent, 1 pcs./bag, 10 pcs./box

Multi-MagPen Drive
Multi-MagPen Sleeve

Cell Repellent Plate(not included in the Kit)

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

IFU F076057 EN [rev.00, 07.2021]

3/ INSTRUCTIONSInstructions to transfer 3D cell cultures using the 96 Well Multi-MagPen
To assure sterility clean the Multi-MagPen Drive with 70 % ethanol. DO NOT autoclave the Multi‑­MagPenDrive (see also safety precautions). During the cellular transfer process, the ­Multi-MegPen Drive is­inserted into the sterile Multi-MagPen Sleeve. Hence the Multi-MagPen Drive is never in direct contact
with the cell culture itself.

1

Prepare Donor Plate
To assure liquid contact and reliable attachmentof the 3D cell cultures to the Multi-MagPen Sleeve
the recommended working volume per well is
100 – 200 μl. If the media volume is too low, theMulti‑MagPen Sleeve will not touch or dip in theliquid and 3D cell cultures will not attach to it. If themedia volume is too high, media will spill out the well
as soon as the Multi-MagPen Sleeve will dip in.

If media must be removed from thewells, the microplate should be placedon the 96 Well Holding Drive (partof the 96 Well Bioprinting Kits; Item
No. 655840/-41) to secure the 3D cell­
cultures at the bottom of the wells during pipetting.

2Prepare Receiver Plate
The receiver plate should contain the same liquidvolume as the donor plate (100 - 200 µl/well). If themedia volume is too low, the Multi-MagPen Sleeve
will not touch or dip in the liquid and 3D cell cultures
will not be transferred properly to the new plate. Ifthe media volume is too high, media will spill out thewell as soon as the Multi-MagPen Sleeve will dip inand 3D cell cultures might get lost.

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

IFU F076057 EN [rev.00, 07.2021]

3

4567

Take off the lid of the donor plate (microwell platecontaining 3D cell cultures or spheroids) and insert
the sterile Multi-MagPen Sleeve in the wells of theplate.

Thereafter insert the magnetic Multi-MagPen
Drive into the Multi-MagPen Sleeve.

Carefully make 2-3 circular movements withthe plate on the desk to facilitate 3D cell cultureattachment to the Multi-MagPen Sleeve.

Gently lift the Multi-MagPen Drive with its sleeve
off the plate. The magnetized 3D cell cultures will
stick to the Multi-MagPen Sleeve and will be heldby the magnetic force of the Multi-MagPen Drive
during transfer.

Place the combined Multi-MagPen Drive and­Multi‑MagPen Sleeve into the receiver plate or­where you want to transfer the cells to.

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

IFU F076057 EN [rev.00, 07.2021]

WARNING
DO NOT place the receiver plate still containing the Multi-MagPen Drive in theMulti‑MagPen Sleeve on the Holding Drive. Due to the magnetic force they
can strongly attract each other, and separation of the components becomesdifficult and may damage the magnet drives.

8Take out the Multi-MagPen Drive from theMulti‑MagPen Sleeve.

9Place the receiver plate containing only theMulti‑MagPen Sleeve on the 96 Well Holding Drive(part of the 96 Well Bioprinting Kits; Item No.
655840/-41). The 3D cell cultures will detach fromthe Multi-MagPen Sleeve by the magnetic force ofthe Holding Drive and settle on the bottom of thewell.

10

Tap lightly with the flat of your hand on theMulti‑MagPen Sleeve. This will mechanically detachthe 3D cell cultures from the Multi-MagPen Sleeve inaddition to the magnetic force of the Holding Drive.
Remove the Multi-MagPen Sleeve and cover thereceiver plate with its original lid.

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

IFU F076057 EN [rev.00, 07.2021]

4/ TROUBLESHOOTING
Problem

Probable CaseA few 3D cell cultures remain in
the donor plate

Remaining 3D cell
cultures did notadhere sufficiently
to the Multi-MagPenSleeve

A few 3D cell cultures remain
attached to the Multi-MagPenSleeve

Remaining 3D cellcultures did adhere
strongly to theMulti-MagPen Sleeve

Put the Multi-MagPen Sleeve againinto the receiver plate (which is still
located on the Holding Drive) andrepeat step 9 and 10 in order toimprove the detachment of the 3D
cell cultures

Inconsistent 3D cell culture
transfer from the donor plateto the receiver plate

Magnetization of the3D cell culture is too
low

Increase concentration of
NanoShuttle

Customer has notpurchased a 96 Well
Bioprinting Kit

The Multi-MagPen Drive can serve
to detach 3D cell cultures fromthe Multi-MagPen Sleeve. Afterremoving the Multi-MagPen Drive
from the Multi-MagPen Sleeve,
position it under the receiver plate
and to use its magnetic force torelease the 3D cell cultures fromthe Multi-MagPen Sleeve

96 Well Holding Drive is notavailable

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE +49 7022 948-0 / FAX +49 7022 948-514 / E-MAIL info@de.gbo.com

Solution
Repeat step 3 to 9
If available, you can use theSingle‑MagPen (Item No. 657850)
for the relocation of single 3D cellcultures

IFU F076057 EN [rev.00, 07.2021]

www.gbo.com
GREINER BIO-ONE GMBHFRICKENHAUSEN, GERMANY
PHONE
FAX
E-MAIL+49 7022 948-0+49 7022 948-514
info@de.gbo.com

Devices of Greiner Bio-One are to be used by properly qualified persons only inaccordance with the relevant Instructions for Use (IFU), where applicable.
For more information contact your local Greiner Bio-One sales representative or visit ourwebsite (www.gbo.com).
All information is provided without guarantee despite careful processing. Any liability, warranty
or guarantee of Greiner Bio-One GmbH is excluded. All rights, errors and changes are reserved.
If not stated otherwise, Greiner Bio-One GmbH has all copyrights and/or other (user-)rights inthis documents, in particular to signs such as the mentioned (word-picture-)brands and logos.
Any use, duplication or any other use of the rights of Greiner Bio-One GmbH is expressly prohibited.
Media owner: Greiner Bio-One GmbH / Represented by Managing Directors Jakob Breuer andHeinz Schmid. The company is registered in the Commercial Register at the first instance courtin Stuttgart, HRB 224604 / VAT Number: DE812585719.

F076057 English [rev.00, 07.2021]

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