Institute of Resource
Development and Analysis
Kumamoto University

About IRDA
The Institute of Resource Development and Analysis (IRDA) was established in Kumamoto
University in 2003. The mission of IRDA is to promote education and research in various
scientiﬁc areas by providing diverse research resources and information.
IRDA was established by merging and functionally reorganizing Center for Animal
Resources and Development (CARD), Gene Technology Center (GTC), Radioisotope
Center (RIC) and three local radioisotope laboratories (Honjo RI, Kurokami RI, Ooe RI).
Those centers and laboratories in IRDA systematically cooperate together to regional,
domestic and international biomedical sciences through the use of research resources
such as genetically engineered animals.
The major missions of IRDA are:1) Production, development, preservation and supply of experimental animals including
genetically engineered mice, and construction and analysis of the databases by using
advanced bioinformatics.
2) Kumamoto Mouse Clinic (KMC): Phenotypic analysis of genetically modiﬁed mice.
3) Research, education, enlightenment, data management and technical support ofexperiments using animals, genetic materials, radioisotopes and various instrument.

Organization

Division ofMicrobiology and Genetics

Laboratory ofClinical Chemistry and Hematology

Division ofTransgenic Technology

Laboratory of Pathology

Division of
Reproductive Engineering

Laboratory of
Cardiovascular system
Laboratory of Respiratory System

Division of Bioinformatics
Laboratory of Nervous System

Division of
Developmental Genetics
Division of Isotope Science
Division of Mouse Clinic

Laboratory of Metabolism
Laboratory of Embryology
Laboratory of Immunology

Center for Animal Resources andDevelopment(CARD)

Ooe Rl Center

Gene Technology Center(GTC)

Honjo Rl Center

Radioisotope Center(RIC)

Kurokami Rl Center

Kumamoto University
Institute of Resource Development and Analysis
Chimeric mouse.
Embryonic stem cells (ES cells).
Isolator.

Research and Development
Development of novel methods for molecularand genetic diagnosis for thecauses of infectious diseases of laboratory animals.
Production and analysis of genetically engineering miceby exchangeable gene-trap method.
Cryopreservation of embryo/spermatozoa.
Production of genetically modiﬁed mice about humandisease model.
Microinjecting DNA into a pronuclei of mouse
fertilized egg.
Analysis of gene function using genetically modiﬁed mice.
Molecular imaging of laboratory animals.
Development of mouse reproductivetechniques.
Husbandry and management
Management of radiation safety.
of laboratory animals.
Genetically engineered mice
production services.
Guide for the use of laboratory animals.
Phenotypic analysis of mice.
Safety training for recombinant
Cryopreservation of mouse embryo
DNA experiment.
and sperm.
Training of mouse reproductive
Experiment handling unsealed radioisotopes.
Collection and distribution of bioresources.
techniques.
Publication of database for archived mice
Education and training for radiation workers.
lines: CARD R-BASE.
Holding of IRDA symposium,
Operation and maintenance of database
CARD seminar and GTC seminar.
for the Exchangeable Gene Trap Clones (EGTC).
Guidance of student practice with RI.
Technical support about radioisotope experiments.
Undergraduate and graduate education.

DNA sequencing.

ResearchSupport

Education

Open House“DNA Museum”.

Community Support

International Mouse Resources include CARD.

Distribution of up-to-date information
about bioresources.
Collaboration with global mouse resource banks.
Education about genome scienceat junior high and high school.
Educational activities about radioisotopes
and radiation.
Holding of workshop for mouse
reproductive technology.
Open House“DNA museum”.

Small animal SPECT/CT system and 99mTc-SPECT/CT images.

Blastocyst injection.

Automated analyzer for clinical chemistry.

Divisions
Division of Microbiology and Genetics
We improve the techniques of animal care and control of pathogens, and perform the following
research projects:
1. Study of oncogenesis for adult T-cell leukemia (ATL) using an animal model anddevelopment of an animal model for evaluation of therapeutic agents for ATL.
2. Development of a novel method for molecular and genetic diagnosis for the causes ofinfectious diseases of laboratory animals.
3. Analysis of intestinal and vaginal ﬂora of experimental animals.

Division of Transgenic Technology
Research support and Technical development related to the production of geneticallyengineered mice.
1) Genetically Engineered Mice Production2) Analysis of mouse models for human diseases
3) Development of new technology and basic research of developmental engineering
technology

Division of Reproductive Engineering
We have provides the service of mouse bank as archiving cryopreserved embryos and sperm ofgenetically modiﬁed mice and rederivation of the mice to scientiﬁc community and developed
mouse reproductive techniques.
1) Collection, reservation and production of genetically engineered mice using mouse
reproductive technology.
2) Development of mouse reproductive techniques.
3) Education of mouse reproductive techniques.

Division of Developmental Genetics
We are developing techniques of genome engineering using mouse ES cells, and producing
humanized mice in which mouse genes with human genes causing genetic disease.
1) Functional analysis of mouse genome in vivo.
2) Developmental genetics of morphogenesis and disease.
3) Establishment of model mice of human diseases and analysis of the pathogenesis.

Division of Bioinformatics
We examine the vital functions of various genes by using genetically modiﬁed mice made byutilizing a gene trap method. We have compiled a database of information on gene trap clones
and keep them as an open-resource to be used by all researchers.
1) Producing and analyzing diseased-model mice using the exchangeable gene trap method.
2) Development and improvement of the Database for the Exchangeable Gene Trap Clones(EGTC).

Division of Radioisotope science
We develop qualiﬁcation method of RI (radioisotope) distribution in vivo and radiation inﬂuencein vivo and research of radiation safety management technology upgrade.
1. The research of imaging technique for in vivo function analysis by RI.
2. Research of stress response to radiation and heat, and molecular mechanism of defense
mechanism.
3. Research of experimental techniques, safety control techniques and facility management inradiation safety management.

Division of Mouse Clinic
We provide an infrastructure for mouse phenotypic analysis, perform specialized diagnostic
examination and educate the basic techniques about mouse phenotypic analysis. Our division isconsisted of 8 laboratories: 1) Clinical Chemistry and Hematology, 2) Pathology,3)Cardiovascular system, 4) Respiratory System, 5) Nervous System, 6) Embryology,7) Metabolism and 8) Immunology

IRDA Oﬃce
2-2-1 Honjo, Chuo-ku, Kumamoto
860-0811, Japan
Tel: 81-96-373-6637, Fax: 81-96-373-6638
E-mail: irda@gtc.gtca.kumamoto-u.ac.jp
URL: http://irda.kuma-u.jp■ Radioisotope Center: RIC
2-2-1 Honjo, Chuo-ku, Kumamoto
860-0811, Japan
Tel: 81-96-373-6512, Fax: 81-96-373-6510

Honjo Radioisotope Laboratory■Center for Animal Resources andDevelopment: CARD

2-2-1 Honjo, Chuo-ku, Kumamoto
860-0811, Japan
Tel: 81-96-373-5377, Fax: 81-96-373-5378

2-2-1 Honjo, Chuo-ku, Kumamoto
860-0811, Japan
Tel: 81-96-373-6548, Fax: 81-96-373-6552

Kurokami Radioisotope Laboratory
2-39-1 Kurokami, Chuo-ku, Kumamoto
860-8555, Japan
Tel: 81-96-342-3782, Fax: 81-96-342-3782■Gene Technology Center: GTC
2-2-1 Honjo, Chuo-ku, Kumamoto
860-0811, Japan
Tel: 81-96-373-6501, Fax: 81-96-373-6502

Ooe Radioisotope Laboratory
5-1 Ooehonmachi, Chuo-ku, Kumamoto
862-0973, Japan
Tel: 81-96-371-4675, Fax: 81-96-371-4675

Honjo-Kuhonji Campus,Ooe Campus,Kurokami Campus

Bus Center
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