•AS19Inhibitors

Product Data Sheet

Screening Libraries

CAS No.:1000578-26-6

Molecular Formula:

C₁₈H₂₅N₃

Molecular Weight:

283.41

Target:5-HT Receptor

Pathway:

GPCR/G Protein; Neuronal Signaling

Storage:

Pure form-20°C

3 years

In solvent-80°C

6 months-20°C

1 monthProteins

HY-103142•Cat. No.:SOLVENT & SOLUBILITY
In Vitro

DMSO : 100 mg/mL (352.85 mM; Need ultrasonic)

Solvent

Mass
1 mg

5 mg

10 mg

1 mM

3.5285 mL

17.6423 mL

35.2846 mL

5 mM

0.7057 mL

3.5285 mL

7.0569 mL10 mM

0.3528 mL

1.7642 mL

3.5285 mL

Concentration
Preparing
Stock Solutions

Please refer to the solubility information to select the appropriate solvent.
In Vivo

1. Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline
Solubility: ≥ 2.5 mg/mL (8.82 mM); Clear solution
2. Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (8.82 mM); Clear solution
3. Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (8.82 mM); Clear solution

BIOLOGICAL ACTIVITY
Description

AS19 is a potent, selective 5-HT7 receptor agonist with an IC50 value of 0.83 nM and a Ki of 0.6 nM. AS19 is selective for 5-HT7
over 5-HT1A, 5-HT1B, 5-HT1D, and 5-HT5A receptors (Kis = 89.7 nM, 490 nM, 6.6 nM and 98.5 nM, respectively). AS19 enhances
memory consolidation and reverses Scopolamine- or Dizocilpine-induced amnesia[1][2][3].

IC₅₀ & Target

Page 1 of 2

Human 5-HT7 Receptor

Human 5-HT7 Receptor

5-HT1A Receptor5-HT1B Receptor

0.83 nM (IC50)

0.6 nM (Ki)89.7 nM (Ki)

490 nM (Ki)

5-HT1D Receptor

5-HT5 Receptor

6.6 nM (Ki)98.5 nM (Ki)
www.MedChemExpress.com

In Vitro

The proliferation of T-cell from parachlorophenylalanine-treated mice is significantly reduced, addition of AS19 (1 μM)
completely restores T-cell proliferation after 48 hours[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AS19 (0.5-10 mg/kg; subcutaneous injection; for 24 hours; male Wistar rats) treatment improves memory consolidation in anautoshaping Pavlovian/instrumental learning task[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:

Male Wistar rats (12-week-old) with autoshaping Pavlovian/instrumental learning task[1]Dosage:

0.5 mg/kg, 1 mg/kg, 5 mg/kg, 10 mg/kg

Administration:

Subcutaneous injection; for 24 hours

Result:Enhanced memory formation in an autoshaping Pavlovian/instrumental learning task.

REFERENCES[1]. Perez-García GS, et al. Effects of the potential 5-HT7 receptor agonist AS 19 in an autoshaping learning task. Behav Brain Res. 2005 Aug 30;163(1):136-40.
[2]. León-Ponte M, et al. Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor. Blood. 2007 Apr 15;109(8):3139-46.
[3]. Brenchat A, et al. 5-HT7 receptor activation inhibits mechanical hypersensitivity secondary to capsaicin sensitization in mice. Pain. 2009 Feb;141(3):239-47.

McePdfHeight

Caution: Product has not been fully validated for medical applications. For research use only.
Tel: 609-228-6898

Fax: 609-228-5909

E-mail: tech@MedChemExpress.com

Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA

Page 2 of 2

www.MedChemExpress.com