Genetic Background Monitoring Report

No.： 101206-15

BRC No.: RBRC04020
Strain Name (Official Nomenclature): B6.129-Dnase2a<tm2Osa>
Strain Type: Mutant strain
Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Donor Strain: R1 (ES cell)
Background Strain: C57BL/6NCrlCrlj
Date: 2010/12/6
ID of animals surveyed
C0001QC02
1(N7<+N1)(Generation ) / Sex
2
Locus
Type Location
FemaleSSLP Chr1:19802051-19802209
B6/B6
1 D1Mit3
SSLP Chr1:Syntenic
B6/B6
2 D1Mit322
SSLP Chr1:73734337-73734492
B6/B6
3 D1Mit19
SSLP Chr1:98827535-98827643
B6/B6
4 D1Mit306
SSLP Chr1:154473511-154473632
B6/B6
5 D1Mit501
SSLP Chr1:176558864-176559001
B6/B6
6 D1Mit150
SSLP Chr2:27277814-27277911
B6/B6
7 D2Mit431
SSLP Chr2:65277459-65277647
B6/B6
8 D2Mit9
SSLP Chr2:106750096-106750239
B6/B6
9 D2Mit206
SSLP Chr2:153798642-153798751
B6/B6
10 D2Mit493
SSLP
Chr2:168761877-168761999
B6/B6
11 D2Mit456
SSLP Chr2:178535250-178535364
B6/B6
12 D2Mit148
SSLP Chr3:31137265-31137413
B6/B6
13 D3Mit151
SSLP Chr3:71816030-71816141
B6/B6
14 D3Mit278
SSLP Chr3:123334565-123334712
B6/B6
15 D3Mit318
SSLP Chr3:148932688-148932790
B6/B6
16 D3Mit293
SSLP Chr4:3584271-3584381
B6/B6
17 D4Mit149
SSLP Chr4:25610387-25610576
B6/B6
18 D4Mit2
SSLP Chr4:63026328-63026475
B6/B6
19 D4Mit17
SSLP Chr4:66843059-66843205
B6/B6
20 D4Mit178SSLP Chr4:123838543-123838630
B6/B6
21 D4Mit308
SSLP Chr4:153093974-153094108
B6/B6
22 D4Mit254
SSLP Chr5:8797672-8797875
B6/B6
23 D5Mit48
SSLP Chr5:Syntenic
B6/B6
24 D5Mit267
SSLP Chr5:89700063-89700172
B6/B6
25 D5Mit398
SSLP Chr5:125309605-125309718
B6/B6
26 D5Mit95
SSLP
Chr5:127286127-127286250
B6/B6
27 D5Mit320
SSLP Chr5:139608988-139609104
B6/B6
28 D5Mit374
SSLP Chr6:55505943-55506037
B6/B6
29 D6Mit316
SSLP Chr6:87375892-87376028
B6/B6
30 D6Mit9
SSLP Chr6:93413943-93414087
B6/B6
31 D6Mit102
SSLP Chr6:114232092-114232201
B6/B6
32 D6Mit55
SSLP Chr6:136372097-136372213
B6/B6
33 D6Mit339
SSLP Chr7:31118079-31118215
B6/B6
34 D7Mit246
SSLP Chr7:52767912-52768056
B6/B6
35 D7Mit81
SSLP Chr7:126237672-126237821
B6/B6
36 D7Mit238
SSLP Chr7:Syntenic
B6/B6
37 D7Mit189
SSLP Chr8:37226054-37226173
B6/B6
38 D8Mit293
Kazuyuki Mekada, Ph.D. (2012/1/30)

Genetic Background Monitoring Report

No.： 101206-15

BRC No.: RBRC04020
Strain Name (Official Nomenclature): B6.129-Dnase2a<tm2Osa>
Strain Type: Mutant strain
Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Donor Strain: R1 (ES cell)
Background Strain: C57BL/6NCrlCrlj
Date: 2010/12/6
ID of animals surveyed
C0001QC02
1(N7<+N1)(Generation ) / Sex
2LocusType Location
FemaleSSLP Chr8:91727514-91727649
B6/B639 D8Mit81
SSLP Chr8:Syntenic
B6/B640 D8Mit14
SSLP Chr9:41695932-41696113
B6/B6
41 D9Mit328
SSLP Chr9:87838840-87839011
B6/B6
42 D9Mit269
SSLP Chr9:115307830-115307967
B6/B6
43 D9Mit214
B6/B6
44 D10Mit282 SSLP Chr10:20654078-20654202
B6/B6
45 D10Mit223 SSLP Chr10:67674940-67675089
SSLP Chr10:91885360-91885556
B6/B6
46 D10Mit11
B6/B6
47 D10Mit180 SSLP Chr10:117587667-117587800
B6/B6
48 D11Mit236 SSLP Chr11:45088548-45088651
B6/B6
49 D11Mit260 SSLP Chr11:61609496-61609590
B6/B6
50 D11Mit219 SSLP Chr11:72132472-72132603
B6/B6
51 D11Mit333 SSLP Chr11:108575096-108575220
B6/B6
52 D12Mit153 SSLP Chr12:35920169-35920310
SSLP Chr12:70900839-70901009
B6/B6
53 D12Mit34
B6/B6
54 D12Mit102 SSLP Chr12:105536654-105536786
SSLP Chr12:Syntenic
B6/B6
55 D12Mit7
B6/B6
56 D13Mit117 SSLP Chr13:37654832-37654949
B6/B6
57 D13Mit179 SSLP Chr13:44860362-44860453
B6/B6
58 D13Mit147 SSLP Chr13:98359080-98359187
SSLP Chr13:120128451-120128643
B6/B6
59 D13Mit35
B6/B6
60 D14Mit259 SSLP Chr14:54729500-54729624
B6/B6
61 D14Mit193 SSLP Chr14:71921427-71921544
B6/B6
62 D14Mit165 SSLP Chr14:106982674-106982807
B6/B6
63 D15Mit136 SSLP Chr15:30776615-30776757
SSLP Chr15:43279930-43280030
B6/B6
64 D15Mit5
SSLP Chr15:74075586-74075734
B6/B6
65 D15Mit29
SSLP Chr15:83586169-83586316
B6/B6
66 D15Mit33
B6/B6
67 D16Mit131 SSLP Chr16:7319135-7319274
SSLP Chr16:38433970-38434154
B6/B6
68 D16Mit59
SSLP Chr16:78953071-78953200
B6/B6
69 D16Mit50
B6/B6
70 D16Mit106 SSLP Chr16:98051757-98051902
B6/B6
71 D17Mit213 SSLP Chr17:16752157-16752280
B6/B6
72 D17Mit108 SSLP Chr17:49744546-49744658
B6/B6
73 D17Mit187 SSLP Chr17:79631462-79631708
B6/B6
74 D18Mit177 SSLP Chr18:41142161-41142331
B6/B6
75 D18Mit184 SSLP Chr18:66978897-66979068
B6/B6
76 D18Mit142 SSLP Chr18:75548607-75548726
Kazuyuki Mekada, Ph.D. (2012/1/30)

Genetic Background Monitoring Report

No.： 101206-15

BRC No.: RBRC04020
Strain Name (Official Nomenclature): B6.129-Dnase2a<tm2Osa>
Strain Type: Mutant strain
Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Donor Strain: R1 (ES cell)
Background Strain: C57BL/6NCrlCrlj
Date: 2010/12/6
ID of animals surveyed
C0001QC02
1(N7<+N1)(Generation ) / Sex2Locus
Type LocationFemale
B6/B6
77 D18Mit216 SSLP Chr18:83585146-83585269
SSLP Chr19:21916083-21916201
B6/B6
78 D19Mit96
SSLP Chr19:37331405-37331552
B6/B6
79 D19Mit88
SSLP Chr19:42295742-42295862
B6/B6
80 D19Mit90
SSLP Chr19:59674330-59674465
B6/B6
81 D19Mit71
B6/B6
82 DXMit166 SSLP ChrX:49036675-49036788
B6/B6
83 DXMit210 SSLP ChrX:69741824-69741934
B6/B6
84 DXMit130 SSLP ChrX:130298712-130298880
1

Generation definitions: N_Number of backcross generations; F_Filial or inbreeding generations; M_Crossintercross breeding generations; ?: Use when the prior breeding history is not known; +_Generation
numbers before the "+" took place in the donating investigator, after the "+", at RIKEN BRC.

2

based on UniSTS annotation of NCBI Build 37

Haplotype combination:
B6/B6_C57BL/6 homozygous allele, 129/129_129 homozygous allele, B6/129_heterozyous allele

Kazuyuki Mekada, Ph.D. (2012/1/30)