For laboratory use only

Product Outline

Conventional rat sperm cryopreservation methods are plagued with a number of issues. Notably, fertilisation rates usingcryopreserved rat sperm are low, and frozen-thawed sperm have poor motility and so advanced techniques such as ICSI
are required for in vitro fertilisation (IVF).
Preservation using CARD FERTIUP Rat Sperm Cryopreservation Agent, on the other hand, enables researchers toobtain superior fertilisation and embryonic development rates in IVF carried out using frozen-thawed sperm.
* Sperm freezing/thawing and IVF must be carried out according to the recommended method.

Product characteristics
1. Enables IVF using frozen-thawed rat sperm
CARD FERTIUP Rat Sperm Cryopreservation Agent enables the cryopreservation of rat sperm while maintaining
the sperm’s fertilisation ability.
Even after thawing, CARD FERTIUP Rat Sperm Cryopreservation Agent makes it possible to obtain superior IVF
fertilisation and embryonic development rates.

Fertilization rate (%)

100

93.7%±1.3
82.1%±1.9

80
60
40200

Fresh sperm

Frozen/Thawed sperm

Fig. IVF fertilisation rates using fresh and frozenthawed rat sperm.

Table. Rate of development of fertilised oocytes derived
from frozen-thawed rat sperm to the blastocyst stage

Bibliography ; Naomi Nakagata, Nobuyuki Mikoda, Satohiro Nakao, Ena Nakatsukasa & Toru Takeo, Establishment ofsperm cryopreservation and in vitro fertilisation protocols for rats. SCIENTIFIC REPORTS, 10:93, 2020.

2. Contributes to huge reductions in rat breeding costs
Anywhere between several to several dozen female rats per strain are required for the cryopreservation of rat
embryos. The maintenance of so many female rats costs a considerable amount of money.
Rat sperm cryopreservation using CARD FERTIUP🄬 Rat Sperm Cryopreservation Agent, however, can be carriedout using only one male rat, and cryopreserved rat sperm can be used to obtain large numbers of embryos. This
will lead to a massive reduction in breeding costs associated with the upkeep of live rats.

3. Can be applied to genetically modified rats
CARD FERTIUP Rat Sperm Cryopreservation Agent enables the cryopreservation of valuable geneticallymodified rat sperm, which can be easily thawed and subsequently returned to live rats via IVF and embryo transfer
as and when required.

※FERTIUP is our registered trademark.

Reproductive Engineering Reagent List
For use in mouse reproductive engineering
CARD FERTIUP Mouse Sperm Cryoroprotectant : CPA
CARD FERTIUP Mouse Sperm Preincubation Medium : PM
CARD MEDIUM® Mouse Fertilization Medium
CARD FERTIUP PM (0.5 mL) and CARD MEDIUM®

STANDARD
0.13 mL / 0.5 mL / 1.0 mL
0.5 mL / 1.0 mL
1 kit
1 set

CARD FERTIUP PM (1.0 mL) and CARD MEDIUM®
CARD HyperOva® Superovulation Reagent for Mice
CARD HyperOva® F.D.
CARD mHTF
CARD KSOMCARD 0.25 M Sucrose
CARD 1M DMSOCARD DAP213
CARD Cold Transport Kit

1 set
0.6 mL / 1.0 mL
1 kit
2.0 mL / 5.0 mL
2.0 mL / 5.0 mL
2.0 mL / 5.0 mL
1.0 mL
0.5 mL / 1.0 mL
1 kit

New Products Reagents and equipment for
use in rat reproductive engineering

For use in rat reproductive engineering

STANDARD

CARD FERTIUP Rat Sperm Cryopreservation

1.5 mLCARD Rat IVF medium - A medium for rat in vitro fertilization -1.0 mL

CARD Rat sperm freezing kit

Kit* These products are for research use only. Use for diagnostic or therapeutic purposes is prohibited.

Information on there products are available on following website. Please visit the site below
for more details.
https://www.cosmobiousa.com/
http://www.kyudo.co.jp/
http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/onlinemanual.html

References
For rat reproductive engineering
・Nakagata, N. SCIENTIFIC REPORTS, 10:93, 2020.
For mouse reproductive engineering
・Takeo, T., Hoshii, T., Kondo, Y., Toyodome, H., Arima, H., Yamamura, KI., Irie, T. andNakagata, N. Biol Reprod. 78(3):546-51,2008.
・Takeo, T and Nakagata, N. Lab Anim. 44 (2) : 132-,2010.
・Nakagata N. Methods Mol Biol. 693: 57-734, 2011.
・Takeo , T. and Nakagata, N., PLOS ONE, 10 (5) , e0128330, 2015
・Takeo , T. and Nakagata, N., Theriogenology, 86 (5), 1341-6, 2016
Manufacturer

Distributor

Technical Support :
fertiup@kyudo.co.jp※FERTIUP is our registered trademark.

E-mail : info@cosmobiousa.com
URL : https://www.cosmobiousa.com