MitoBrilliant
Product Guide™

Next-generation fluorescent
stains for the localization andtracking of mitochondria

MitoBrilliant
Technology
Overview™

Fluorescent probes that accumulate specifically incellular organelles are valuable components of the‘toolkit’ for fluorescence imaging and flow cytometry.
Selectively labeling mitochondria with fluorescentprobes is of particular interest due to the importantrole of mitochondria in many cellular processes and in
overall cell health.
Functioning mitochondria underpin many criticalcellular processes, while mitochondrial dysfunction
is linked to numerous diseases. The mitochondrial
membrane potential (Δψm) is an indicator of
mitochondrial activity and health because it relates
directly to the cells’ ability to generate ATP and
Reactive Oxygen Species (ROS). Mild depolarizationof the inner mitochondrial membrane is thought tobe the antioxidant mechanism in mouse embryos. On
the other hand, mitochondrial permeability transition(MPT) induced depolarization in cells during nutrient
deprivation triggers selective mitophagy. Fluorescentprobes that accumulate in the mitochondria specificallyas a result of the transmembrane potential can therefore
provide insights into mitochondrial and cell health.
Sensitive, selective and dynamic labeling of
mitochondria with fluorescent probes is desirable, yetexisting probes often have limitations such as toxicity/
phototoxicity or interference with mitochondrial
functions. Data validating the use of the probes
in different experimental protocols is also often
incomplete. MitoBrilliant probes have been developed
to overcome some common limitations encounteredwith standard mitochondrial trackers, offering
clearer answers to scientific questions. They are nextgeneration fluorescent stains for the localization andtracking of mitochondria in both live and fixed cells.
The range harnesses Janelia Fluor® dye technology,
conferring some of the properties that make thesewidely used dyes, into mitochondrial stains. Two typesof MitoBrilliant probes are available.

2The MitoBrilliant™ Live dyes accumulate in the
mitochondria of live cells in a mitochondrial membranepotential (Δψm) dependent manner. Upon loss of themitochondrial membrane potential, the dyes disperse, andso provide a dynamic assessment of Δψm in live cells. Twodyes are available: MitoBrilliant™ Live 646 (red emission)and MitoBrilliant™ Live 549 (yellow/orange emission).

MitoBrilliant™ 646 is a universal stain for mitochondria
in both live and fixed cells. It is retained in mitochondria
following fixation with exceptionally clear staining
and is retained in mitochondria following loss of themitochondrial membrane potential. Additionally,
MitoBrilliant™ 646 can be used to stain pre-fixed cells andtissue, making this dye suitable for IHC and ICC.

(

•

•

(~

Outer Membrane

H+

Matrix Space

Tom40

H+

~H

~

~
ei~

H+

H+
H+

Inter-membrane
Space

8

H+

+

H+

40

,)

H+

~

H+

-

Tom20

~
40

Inner Membrane

H+

ATP

H+

FIGURE 1. Mitochondrial structure. Illustration of basic mitochondrial structure.

3~

Electron
Transport
Chain

Porin

0

H+

ADP

DNA

. •

H+

0

Transporter

•

H

To
m

Intermembrane Space

~

40

+

Tom

Crista

To
m

Tom40

Inner Membrane◊+_

ATP Synthase

Ribosome-~O..
•

Granules

-,~o•
.. .-

m
m

H+

Matrix·••f-._

Outer MembraneConfocal

STED

MitoBrilliant™ 646

Janelia Fluor® Technology
Developed by Professor Luke Lavis and his team at
the Janelia Research Campus, Janelia Fluor® (JF)dyes provide scientists with an exceptional palette ofbright, photostable fluorophores for a broad range ofapplications.
Janelia Fluor® dye key features and applications:• Exceptionally bright
Tom40• Cell permeable• Highly photostable• Supplied with a choice of reactive groups for
simple biomolecule conjugation• Can be converted to relevant substrate for use in
self-labeling tag systems, e.g. HaloTag® and
SNAP-tag®
MitoBrilliant™ 646
Tom40• Suitable for use in confocal microscopy, IHC, ICC
and flow cytometry• Especially well-suited to live-cell imaging• Ideal for super-resolution techniques including
STED and dSTORM• Photoactivatable Janelia Fluor ® dyes compatible
with PALM microscopy

FIGURE 2: Confocal and STED microscopy images of COS-7 cells stainedwith MitoBrilliant™ 646 (100 nM, magenta) for 30 minutes followed by
immediate fixation with 6% PFA for 20 minutes at 37°C. Samples were further
processed for immunofluorescent antibody staining (outer mitochondrial
membrane protein Tom40, green). Scale bar = 1 µm.

TABLE 1. Key features of MitoBrilliant™ dyes

Product NameCore Dye
Structure

MitoBrilliant™ 646
Cat. No. 7700

Janelia Fluor®
technology

MitoBrilliant™ Live 646
Cat. No. 7417

Janelia Fluor®
technology

MitoBrilliant™ Live 549
Cat. No. 7693

Janelia Fluor®
technology

Abs/Em(nm)

655/668648/662550/568Δψm
Dependent

Live/
Fixed
Cell Use?

No*

Suitablefor live andfixed cell
work

Yes

Live-cellwork only

Yes

Live-cellwork only

Stains
Pre-Fixed
Cells?

Image
Without
Wash Step?

Demonstrated
Applications

Yes

Yes, but
replacing media
recommended

Fixed-cell imaging,Live-cell imaging
Flow cytometry,
IHC/ICC,
Super-resolution,
microscopy – STED,
High-content
screeningNo

Yes, but
replacing media
recommended

Live-cell imaging,
Flow cytometry,High-content
screeningNo

Yes, but
replacing media
recommended

Live-cell imaging,
Flow cytometry,High-content
screening* In live-cell staining, Δψm drives initial recruitment of the dye into mitochondria. After staining, localization of the dyes becomes insensitive to Δψm changes. In pre-fixedcell staining, MitoBrilliant™ 646 can localize and stay in mitochondria without Δψm (see FIGURE 6).

4

MitoBrilliant™ 646(Cat. No. 7700)
Highly versatile ‘universal stain’ for both live and fixedcell applications• Microscopy/Imaging• Flow cytometry• IHC/ICC• Super-resolution microscopy – STED
• High-content imaging/screening

Emission Color

100

~
p
·enC
(l)

+'

C

80

>

:,=,

ro

0::

40

20

300

400

500

600

700

800

Wavelength (nm)
Excitation- - Emission

FIGURE 3. Fluorescence excitation and emission spectra for MitoBrilliant™ 646.

5655 nmλem

668 nmClosest Laser Line

(l)

OJ

λabs

Extinction Coefficient (ε)

60

900

Red

125,000 M-1 cm-1
640 nmMitoBrilliant™ 646(Cat. No. 7700)
Application Data: Confocal Live-Cell Imaging
MitoBrilliant™ 646 is highly cell permeable, staining is stable
and it is retained in mitochondria after a washout step. These
properties make it an ideal choice for confocal live-cell
imaging (see FIGURE 4).
In addition, after staining, MitoBrilliant™ 646 is insensitive tochanges in mitochondrial membrane potential (see FIGURE 7).

FIGURE 4. Mitochondria morphological examination at higher resolution
in live cells. HeLa cells were incubated with MitoBrilliant™ 646 (100 nM) for
40 minutes. Cells were counterstained with DAPI (Cat. No. 5748). Image wastaken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.

MitoBrilliant™ 646 staining is retained after fixation
with PFA and provides significantly improved staining
resolution than a leading competitor dye.

MitoBrilliant™ 646

Property: Performance and Staining
Fidelity Post-Fixation

10 µm

FIGURE 5. Performance of MitoBrilliant™ 646 after fixation. HeLa cells
were incubated with MitoBrilliantTM 646 (100 nM) for 40 minutes. Cells werethen fixed for 10 minutes in 4% PFA. All images were taken using the LSM880
Confocal using a 63x oil objective. Laser power and gain were set to eachindividual probe. Scale bar = 10 µm.

6Competitor Dye

10 µm

10 µm

10 µm

MitoBrilliant™ 646(Cat. No. 7700)(A)

HoechstCompetitor Dye

Property: Suitability for Staining Pre-Fixed Cells
The data in FIGURE 6 demonstrates that MitoBrilliant™ 646 is able totarget and stain mitochondria in PFA fixed cells, and the staining isasspecific as a leading competitor dye (applied before fixation).

MitoBrilliant™ 646

Competitor Dye
MitoBrilliant™ 646

Competitor Dye

MitoBrilliant™ 646(B)

FIGURE 6. Validation of MitoBrilliant™ 646 for staining pre-fixed cells. (A) Competitor dye (250 nM) was added to HeLa cells 45 minutes prior to fixation. Cells
were fixed in 4% PFA for 20 minutes cells were not permeabilized. MitoBrilliant™ 646 (75 nM) was then applied and the cells were imaged. The merged image
shows the colocalization of the competitor dye (λabs = 578 nm, λem = 598 nm. Imaging setup: Ex: 561 nm, Em: BP 617/73 nm) and MitoBrilliant™ 646 (λabs = 655 nm,
λem = 668 nm. Imaging setup: Ex: 640 nm, Em: BP 685/40 nm). (B) The intensity profiles of competitor dye (green line, applied to live cells prior to fixation) and
MitoBrilliant™ 646 (blue line, applied to the same sample after fixation) are shown. Intensity profiles were taken across a set frame (left) on a single cell andcompared (right). The data shows very high similarity on staining profile, demonstrates that MitoBrilliant™ 646 can be applied post-fixation. Experiments and analysis
were performed by PhenoVista.

77

MitoBrilliant™ 646(Cat. No. 7700)

For live-cell staining, the mitochondrial membrane potential (Δψm)
is only required for initial recruitment of MitoBrilliant™ 646 tothe mitochondria. Subsequent retention is insensitive to loss ofΔψm. This is demonstrated in FIGURE 7 by using carbonyl cyanide
m-chlorophenyl hydrazone (CCCP, Cat. No. 0452) to disrupt Δψmbefore or following staining. MitoBrilliantTM 646 is therefore an
ideal dye for stable labeling of mitochondria during cellular events
that might affect Δψm.

(B)
CCCP Chase(C)
CCCP Pretreatment

Competitor Dye

MitoBrilliant™ 646(A)
No CCCP

Property: Δψm Dependency

FIGURE 7. Δψm Dependency of MitoBrilliant™ 646. COS-7 cells were stained with MitoBrilliant™ 646 (100 nM) or a leading competitor dye (50 nM) for 60 minutes.
Cells were treated with 20 µM CCCP at different stages of staining. Column A: No CCCP treatment. Column B: CCCP treatment applied only after the
mitochondrial staining step, for 30 minutes. Only the MitoBrilliant 646 signal remained, suggesting that this dye was retained in mitochondria even after the loss ofthe membrane potential, whereas the accumulation of the competitor dye was reversible. Column C: CCCP was added 30 minutes before the staining step.
Excessive dye was then washed out with media containing CCCP for 30 minutes. Mitochondrial accumulation of both MitoBrilliant™ 646 and the competitor dye
were abolished. Scale bar = 10 µm.

8MitoBrilliant™ 646(Cat. No. 7700)

HoechstApplication Data: High-Content Imager Data
in Different Cell Types
MitoBrilliant™ 646 reliably produces high quality data in
different cell types as shown in FIGURE 8.

MitoBrilliant™ 646

Competitor Dye

Hoechst
MitoBrilliant™ 646

HeLa

SK-N-SH

A431

FIGURE 8. High-content imager data of mitochondria in different cell types. Cells were incubated with either MitoBrilliant™ 646 (75 nM) or a leading Competitordye (250 nM) for 45 minutes and washed in DMEM complete media prior to live imaging. Cells were counterstained with DAPI (Cat. No. 5748). Images taken using a
Yokogawa CQ1 high-content imager using a 60x objective. Experiments and analysis performed by PhenoVista.

9MitoBrilliant™ 646(Cat. No. 7700)
Application Data: Flow Cytometry
For live-cell staining, MitoBrilliant™ 646 is highly cellpermeable with unique properties relating to Δψmsensitivity, it is therefore a valuable tool for various flow
cytometry experiments.

FIGURE 9. Flow cytometry histogram of cell populations following
MitoBrilliant™ 646 staining. K562 cells were stained with MitoBrilliant™ 646 for
30 minutes at 37˚C. Cells were then washed, and the data were acquired on BD
Fortessa Flow Cytometer using 640 nm laser for excitation and 670/14 nm
emission filter. Data shows overlay of stained (orange) and unstained cells (blue).

Application Data:
Immunohistochemistry (IHC)

Piriform Cortex

MitoBrilliant™ 646 can stain mitochondria either at the prefixation or post-fixation stages of sample preparation for
IHC as shown in FIGURE 10.

Hypothalamus

FIGURE 10. Fluorescence IHC was performed on 10 µm thick rat brain tissue sections followed by staining with MitoBrilliantTM 646. Labeling forphospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) (pseudo-colored green) combined with MitoBrilliant™ 646 (pseudo-colored red). Hypothalamus: labeling fornNOS (pseudo-colored green) combined with MitoBrilliant™ 646 (pseudo-colored red). Tissue sections were stained with DAPI (Cat. No. 5748) nuclear
counterstain (blue).

10

MitoBrilliant™ 646(Cat. No. 7700)

Application Data: Super-Resolution
Microscopy (STED)Super-resolution microscopy (SRM) enables image
generation at resolutions beyond the optical diffraction
limit (~200 nm). Stimulated Emission Depletion (STED)
microscopy is an SRM technique that can provide single
molecule resolution data with appropriate fluorophores.

FIGURE 11. Comparison of confocal and STED data for
MitoBrilliant™ 646. COS-7 cells stained with MitoBrilliant™ 646 (100 nM)
for 30 minutes followed by immediate fixation with 6% PFA for 20
minutes at 37 °C. Shown are confocal (left) and super-resolution (right,
20 nm pixel size) images of mitochondria. Scale bar = 1 µm.

11MitoBrilliant™ 646
Tom40

Tom40

MitoBrilliant™ 646

ConfocalFIGURE 12. Colocalization of MitoBrilliant™ 646 with Tom40
using STED microscopy. COS-7 cells dual-stained with
MitoBrilliant™ 646 (100 nM, magenta) for 30 minutes followed by
immediate fixation with 6% PFA for 20 minutes at 37 °C. Samples
were further processed for immunofluorescent antibody staining(outer mitochondrial membrane protein Tom40, green). Shownare confocal (left) and super-resolution (right), 20 nm pixel size
images of mitochondria. Scale bar = 1 µm.

STED

MitoBrilliant™ 646

Confocal

STEDMitoBrilliant™ Live 646(Cat. No. 7417)
Photostable, Δψm dependent mitochondrial dye forlive-cell applications• Microscopy/Imaging• Flow cytometry• High-content imaging/screening

Emission Color

80
~
C
+-'

E

60

QJ

>
ro

:;:::;

ai

0:

40

300

400

500

600

700

800

Wavelength (nm )Exci tation- - Emission

FIGURE 13. Fluorescence excitation and emission spectra for MitoBrilliant™ Live 646.

12648 nmλem

662 nmClosest Laser Line

"iii
QJ

λabs

Extinction Coefficient (ε)

~

900

Red

127,800 M-1 cm-1
640 nmMitoBrilliant™ Live 646(Cat. No. 7417)

Property: Mitochondrial Specificity
The following dataset provides experimental data showcasing the
key properties of MitoBrilliant™ Live 646 and its utility indifferent applications.
Using mitochondrial outer membrane protein Tom20 as the
localization marker, FIGURE 14 shows that MitoBrilliant™ Live 646
specifically accumulates in mitochondria in live cells without any
detectable off-target staining.

Tom20-EGFP

Merge

100 nM

MitoBrilliant™ Live 646

200 nM

10 µm

10 µm

FIGURE 14. Colocalization of MitoBrilliantTM Live 646 with Tom20. COS-7 cells stably expressing human Tom20-EGFP were stained with 100 nM (top panel) or
200 nM (lower panel) MitoBrilliant™ Live 646 for 30 minutes followed by a washout step with fresh media. MitoBrilliant™ Live 646 localization matched closely with
Tom20-EGFP indicating specific staining of the mitochondria. Scale bar = 10 µm.

13MitoBrilliant™ Live 646(Cat. No. 7417)

Property: Δψm Dependency
Mitochondrial membrane potential (Δψm) is required for both
initial recruitment and subsequent retention of MitoBrilliant™
Live 646 in the mitochondria of live cells.
This is demonstrated in FIGURE 15 by using CCCP (Cat. No.
0452) to disrupt Δψm before and following treatment with
MitoBrilliant™ Live 646.

(C)
CCCP Pretreatment

Merge with Tom20-EGFP

Probe Only

(B)
CCCP Chase

10 µm

Probe Only
Merge with Tom20-EGFP

Competitor Dye

MitoBrilliant™ Live 646(A)
No CCCP

10 µm

FIGURE 15. Δψm dependency. COS-7 cells expressing Tom20-EGFP were stained with MitoBrilliant™ Live 646 (100 nM) or a leading competitor dye (50 nM) for 60
minutes. Cells were treated with 20 µM CCCP (Cat. No. 0452) to disrupt Δψm at different stages of staining Column A: No CCCP treatment. Column B: CCCP
treatment applied only after the mitochondrial staining step, for 30 minutes causing the loss of MitoBrilliant Live 646 and competitor dye signal. Column C: CCCP
was added 30 minutes before the staining step. Excessive dye was then washed out with media containing CCCP for 30 minutes. No staining observed for either dye.

14

Property: Photostability

MitoBrilliant™ Live 646(Cat. No. 7417)

In live-cell time lapse imaging, MitoBrilliant™ Live 646
produced a more stable, longer-lasting fluorescent signal
than a leading competitor dye.

Frames / time (s)
100

200

300

Competitor Dye

MitoBrilliant™ Live 646

0

FIGURE 16. Fluorescence intensity changes during live-cell imaging. COS-7 cells were stained with MitoBrilliant™ Live 646 (100 nM) or a leading competitordye (50 nM) for 60 minutes followed by the washout of excess dye with fresh media. Images were acquired every second over a time frame of 5 minutes. Shown
are representative images after 0, 100, 200 and 300 seconds.

Application Data: Confocal Live-Cell Imaging
MitoBrilliant™ Live 646 is highly cell permeable, stable and isretained in mitochondria due to the membrane potential after awashout step. These properties make MitoBrilliant™ Live 646 an
ideal choice for confocal live-cell imaging.

FIGURE 17. Mitochondria morphological examination at higher resolution inlive cells. HeLa cells incubated with MitoBrilliant™ Live 646 (100 nM) for 40
minutes. Cells were counter stained with DAPI (Cat. No. 5748). Image taken using
an LSM880 Confocal using a 63X objective. Scale bar = 10 µm.

15

10 µm

MitoBrilliant™ Live 646(Cat. No. 7417)

HoechstApplication Data: High-Content Imager Datawith Different Cell Types
MitoBrilliant™ Live 646 reliably produces high quality data in
different cell types as shown in FIGURE 18.

MitoBrilliant™ Live 646

Competitor Dye

Hoechst
MitoBrilliant™ Live 646

HeLa

SK-N-SH

A431

FIGURE 18. High-content imager data of mitochondrial staining in different cell types. Cells were incubated with either MitoBrilliant™ Live 646 (75 nM) or aleading competitor dye (250 nM) for 45 minutes and washed in DMEM complete media prior to live imaging. Cells were counter-stained with Hoechst. Images
taken using a Yokogawa CQ1 high-content imager using a 60X objective. Experiments and analysis performed by PhenoVista.

Application Data: Flow Cytometry
MitoBrilliant™ Live 646 is highly cell permeable, staining is stable and it
is retained in mitochondria after a washout step. It can be used to stain
cells in culture flasks or in suspension solution. These properties make
MitoBrilliant™ Live 646 an ideal choice for flow cytometry.

FIGURE 19. Flow cytometry histogram of cell populations following MitoBrilliant™ Live 646
staining. K562 cells were stained with MitoBrilliant™ Live 646 for 30 minutes at 37˚C. Cells were thenwashed and data were acquired on BD Fortessa Flow Cytometer using 640 nm laser for excitation
and 670/14 nm emission filter. Data show overlay of stained (orange) and unstained cells (blue).

16

MitoBrilliant™ Live 549(Cat. No. 7693)
Exceptionally bright mitochondrial dye for live-cell applications• Microscopy/Imaging• Flow cytometry• High-content imaging/screening

Emission Color

100

80

λabs

550 nmλem

568 nmExtinction Coefficient (ε)
Closest Laser Line

CJ)

C
(1)

1=

60

(1)

>
co

·.;::,

ID
0::

40

20

"-----300

400

500

600

700

800

900

Wavelength (nm)
Excita ti on- - Emission

FIGURE 20. Fluorescence excitation and emission spectra for MitoBrilliant™ Live 549.

17

Yellow/ Orange

100,000 M-1 cm-1
532 nmMitoBrilliant™ Live 549(Cat. No. 7693)

Property: Brightness
The following dataset provides experimental data showcasing the
key properties of MitoBrilliant™ Live 549 and its utility in different
applications.
Derived from Janelia Fluor® technology, MitoBrilliant™ Live 549 issignificantly brighter than a leading competitor dye with similar
maximum absorption and emission wavelength (see FIGURE 21).
It provides exceptional data in both live-cell imaging and flow
cytometry.

MitoBrilliant™ Live 549

Leading Competitor Dye

10 µm

10 µm

FIGURE 21. Mitochondrial probe brightness comparison. HeLa cells where incubated with MitoBrilliant™ Live 549 (100 nM, left) or a leading competitor dye (100nM, right) for 40 minutes. Cells were counterstained with DAPI (Cat. No. 5748). Image taken using an LSM880 Confocal using a 63X objective with the same setting
for both images. Scale bar = 10 µm.

Application: Flow Cytometry
MitoBrilliant™ Live 549 is highly cell permeable, staining
is stable and it is retained in mitochondria due to themembrane potential after a washout step. It can be used tostain cells in culture flasks or in suspension solution. These
properties make MitoBrilliant™ Live 549 an ideal choice forflow cytometry.

FIGURE 22. Flow cytometry histogram of cell populations following
MitoBrilliant™ Live 549 staining. K562 cells were stained with MitoBrilliant™ Live549 for 30 minutes at 37˚C. Cells were then washed, and data were acquired on
BD Fortessa Flow Cytometer using 561 nm laser for excitation and 586/15 nm
emission filter. Data show overlay of stained (orange) and unstained cells (blue).

18

Staining Live Cells

Staining Fixed Cells

Applicable toApplicable to MitoBrilliant™ 646 (Cat. No. 7700), which
can be applied at either pre-fixation (FIGURE 5) or postfixation (FIGURE 6) stages of sample preparation.

• MitoBrilliant™ 646 (Cat. No. 7700)• MitoBrilliant™ Live 646 (Cat. No. 7417)• MitoBrilliant™ Live 549 (Cat. No. 7693)

Prepare a 1 mM MitoBrilliant stock solution withDMSO (see the table below for recommended
reconstitution). We recommend that stock solutions,once prepared, are stored aliquoted in tightly sealed
vials at -20°C or below and used within 1 month.
™

• Dilute the DMSO stock solution using a warm
(37°C) buffer or growth medium and apply to live
cells at a final working concentration between
50-200 nM. We recommend optimizing the final
concentration used for individual experiments.
Aqueous working solutions should be preparedand used on the same day.
• Incubate for 30-60 minutes at 37°C prior to
imaging (longer incubation times may givebrighter staining). A washing step prior to imaging
is not required but it is recommended: rinse thecells with 1x PBS and apply fresh media prior to
imaging.

• Dilute the 1 mM DMSO stock solution to a final
working concentration between 50 to 200 nM. We
recommend optimizing the final concentration
used for individual experiments. Aqueous working
solutions should be prepared and used on thesame day.
• Appropriate fixation methods are critical forpreserving cellular structure and achieving optimal
staining. For HeLa cells, we recommend fixing withfreshly prepared pre-warmed buffer or growth
medium containing 4% paraformaldehyde at 37°C for
10 to 20 minutes. Optimization might be required for
different model systems. After fixation, rinse the cells
several times in PBS.
• Permeabilization step (optional). Incubate fixed
cells for 10 minutes in PBS containing 0.05% Triton®
X-100. Staining cells before permeabilization isrecommended for getting the best results (seeFIGURE 10 and FIGURE 12).

Staining cells in suspensions for flow cytometry:
After gentle centrifugation, carefully resuspend thecell pellet in a prewarmed (37°C) buffer or growth
medium containing 50-200 nM MitoBrilliant™ at a cellconcentration of 1 x 106 per mL. After 30-60 minutes
incubation at 37°C in the dark, re-pellet the cells by
gentle centrifugation, then resuspend cells in fresh
pre-warmed medium ready for flow cytometry.

Product Name

Molecular
WeightQuantity Per Vial

Recommended Reconstitution

MitoBrilliant™ 646
Cat. No. 7700

493.6

50 µg (101 nmoles)

Add 101 µL DMSO for a 1 mM stock solution

MitoBrilliant™ Live 646
Cat. No. 7417

445.1

50 µg (112 nmoles)

Add 112 µL DMSO for a 1 mM stock solution

MitoBrilliant™ Live 549
Cat. No. 7693

417.0

50 µg (120 nmoles)

Add 120 µL DMSO for a 1 mM stock solution

19

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