Genetic Background Monitoring Report

No.： 100616-24

BRC No.: RBRC00858
Strain Name (Official Nomenclature): B6;129S6-Irf3<tm1Ttg>
Strain of Origin: Mutant Stock
Strain of origin: 129S6/SvEv
Donor strain: EK.CCE (ES cell)
Background strain: C57BL/6J and 129 mixed
Date: 2010/6/16
ID of animals surveyed
S00330204 S00330209
1(N8?+F28) (N8?+F28)(Generation ) / Sex
Locus
Type Location2
FemaleMale
SSLP Chr1:19802051-19802209
1 D1Mit3
B6/B6
B6/B6
SSLP Chr1:Syntenic
2 D1Mit322
B6/B6
B6/B6
SSLP Chr1:73734337-73734492
3 D1Mit19
B6/B6
B6/B6
SSLP Chr1:98827535-98827643
4 D1Mit306
B6/B6
B6/B6
SSLP Chr1:154473511-154473632
5 D1Mit501
B6/B6
B6/B6
SSLP Chr1:176558864-176559001
6 D1Mit150
B6/B6
B6/B6
SSLP Chr2:27277814-27277911
7 D2Mit431
129/129
129/129
SSLP Chr2:65277459-65277647
8 D2Mit9
B6/B6
B6/B6
SSLP Chr2:106750096-106750239
9 D2Mit206
129/129
129/129
SSLP Chr2:153798642-153798751
10 D2Mit493
B6/B6
B6/B6
SSLP Chr2:168761877-168761999
11 D2Mit456
B6/B6
B6/B6
SSLP Chr2:178535250-178535364
12 D2Mit148
B6/B6
B6/B6
SSLP Chr3:31137265-31137413
13 D3Mit151
129/129
129/129
SSLP Chr3:71816030-71816141
14 D3Mit278
129/129
129/129
SSLP Chr3:123334565-123334712
15 D3Mit318
B6/B6
B6/B6
SSLP Chr3:148932688-148932790
16 D3Mit293
B6/B6
B6/B6
SSLP Chr4:3584271-3584381
17 D4Mit149
B6/B6
B6/B6
SSLP Chr4:25610387-25610576
18 D4Mit2
B6/B6
B6/B6
SSLP Chr4:63026328-63026475
19 D4Mit17
129/129
129/129
SSLP Chr4:66843059-66843205
20 D4Mit178
129/129
129/129
SSLP Chr4:123838543-123838630
21 D4Mit308
B6/B6
B6/B6
SSLP Chr4:153093974-153094108
22 D4Mit254
B6/B6
B6/B6
SSLP Chr5:8797672-8797875
23 D5Mit48
B6/B6
B6/B6
SSLP Chr5:Syntenic
24 D5Mit267
B6/B6
B6/B6
SSLP Chr5:89700063-89700172
25 D5Mit398
129/129
129/129
SSLP Chr5:125309605-125309718
26 D5Mit95
B6/B6
B6/B6
SSLP Chr5:127286127-127286250
27 D5Mit320
B6/B6
B6/B6
SSLP Chr5:139608988-139609104
28 D5Mit374
B6/B6
B6/B6
SSLP Chr6:55505943-55506037
29 D6Mit316
B6/B6
B6/B6
SSLP Chr6:87375892-87376028
30 D6Mit9
B6/B6
B6/B6
SSLP Chr6:93413943-93414087
31 D6Mit102
B6/B6
B6/B6
SSLP Chr6:114232092-114232201
32 D6Mit55
B6/B6
B6/B6
SSLP Chr6:136372097-136372213
33 D6Mit339
B6/B6
B6/B6
SSLP Chr7:31118079-31118215
34 D7Mit246
129/129
129/129
SSLP Chr7:52767912-52768056
35 D7Mit81
129/129
129/129
SSLP Chr7:126237672-126237821
36 D7Mit238
129/129
129/129
SSLP Chr7:Syntenic
37 D7Mit189
129/129
129/129
SSLP Chr8:37226054-37226173
38 D8Mit293
B6/B6
B6/B6
Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report

No.： 100616-24

BRC No.: RBRC00858
Strain Name (Official Nomenclature): B6;129S6-Irf3<tm1Ttg>
Strain of Origin: Mutant Stock
Strain of origin: 129S6/SvEv
Donor strain: EK.CCE (ES cell)
Background strain: C57BL/6J and 129 mixed
Date: 2010/6/16
ID of animals surveyed
S00330204 S00330209
1(N8?+F28) (N8?+F28)(Generation ) / Sex
Locus
Type Location2
FemaleMale
SSLP Chr8:91727514-91727649
39 D8Mit81
B6/B6
B6/B6
SSLP Chr8:Syntenic
40 D8Mit14
B6/B6
B6/B6
SSLP Chr9:41695932-41696113
41 D9Mit328
B6/B6
B6/B6
SSLP Chr9:87838840-87839011
42 D9Mit269
SSLP Chr9:115307830-115307967
43 D9Mit214
129/129
129/12944 D10Mit282 SSLP Chr10:20654078-20654202
B6/B6
B6/B6
45 D10Mit223 SSLP Chr10:67674940-67675089
B6/B6
B6/B6
SSLP Chr10:91885360-91885556
46 D10Mit11
B6/B6
B6/B6
47 D10Mit180 SSLP Chr10:117587667-117587800
B6/B6
B6/B6
48 D11Mit236 SSLP Chr11:45088548-45088651
49 D11Mit260 SSLP Chr11:61609496-61609590
129/129
129/12950 D11Mit219 SSLP Chr11:72132472-72132603
B6/B6
B6/B6
51 D11Mit333 SSLP Chr11:108575096-108575220
129/129
129/12952 D12Mit153 SSLP Chr12:35920169-35920310
SSLP Chr12:70900839-70901009
53 D12Mit34
129/129
129/129
54 D12Mit102 SSLP Chr12:105536654-105536786
B6/B6
B6/B6
SSLP Chr12:Syntenic
55 D12Mit7
B6/B6
B6/B6
56 D13Mit117 SSLP Chr13:37654832-37654949
B6/B6
B6/B6
57 D13Mit179 SSLP Chr13:44860362-44860453
B6/B6
B6/B6
58 D13Mit147 SSLP Chr13:98359080-98359187
B6/B6
B6/B6
SSLP Chr13:120128451-120128643
59 D13Mit35
129/129
129/12960 D14Mit259 SSLP Chr14:54729500-54729624
129/129
129/129
61 D14Mit193 SSLP Chr14:71921427-71921544
129/129
129/12962 D14Mit165 SSLP Chr14:106982674-106982807
B6/B6
B6/B6
63 D15Mit136 SSLP Chr15:30776615-30776757
B6/B6
B6/B6
SSLP Chr15:43279930-43280030
64 D15Mit5
B6/B6
B6/B6
SSLP Chr15:74075586-74075734
65 D15Mit29
B6/B6
B6/B6
SSLP Chr15:83586169-83586316
66 D15Mit33
B6/B6
B6/B6
67 D16Mit131 SSLP Chr16:7319135-7319274
B6/B6
B6/B6
SSLP Chr16:38433970-38434154
68 D16Mit59
B6/B6
B6/B6
SSLP Chr16:78953071-78953200
69 D16Mit50
129/129
129/12970 D16Mit106 SSLP Chr16:98051757-98051902
B6/B6
B6/B6
71 D17Mit213 SSLP Chr17:16752157-16752280
B6/B6
B6/B6
72 D17Mit108 SSLP Chr17:49744546-49744658
B6/B6
B6/B6
73 D17Mit187 SSLP Chr17:79631462-79631708
129/129
129/129
74 D18Mit177 SSLP Chr18:41142161-41142331
B6/B6
B6/B6
75 D18Mit184 SSLP Chr18:66978897-66979068
B6/B6
B6/B6
76 D18Mit142 SSLP Chr18:75548607-75548726
B6/B6
B6/B6
Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report

No.： 100616-24

BRC No.: RBRC00858
Strain Name (Official Nomenclature): B6;129S6-Irf3<tm1Ttg>
Strain of Origin: Mutant Stock
Strain of origin: 129S6/SvEv
Donor strain: EK.CCE (ES cell)
Background strain: C57BL/6J and 129 mixed
Date: 2010/6/16
ID of animals surveyed
S00330204 S00330209
1(N8?+F28) (N8?+F28)(Generation ) / Sex
Locus
Type Location2
FemaleMale
77 D18Mit216 SSLP Chr18:83585146-83585269
B6/B6
B6/B6
SSLP Chr19:21916083-21916201
78 D19Mit96
B6/B6
B6/B6
SSLP Chr19:37331405-37331552
79 D19Mit88
129/129
129/129
SSLP Chr19:42295742-42295862
80 D19Mit90
129/129
129/129
SSLP Chr19:59674330-59674465
81 D19Mit71
B6/B6
B6/B6
82 DXMit166 SSLP ChrX:49036675-49036788
B6/B6
B6/B6
83 DXMit210 SSLP ChrX:69741824-69741934
B6/B6
B6/B6
84 DXMit130 SSLP ChrX:130298712-130298880
1

Generation definitions: N_Number of backcross generations; F_Filial or inbreeding generations; M_Crossintercross breeding generations; ?: Use when the prior breeding history is not known; +_Generation
numbers before the "+" took place in the donating investigator, after the "+", at RIKEN BRC.
2
based on UniSTS annotation of NCBI Build 37
Haplotype combination:
B6/B6_C57BL/6 homozygous allele, 129/129_129 homozygous allele, B6/129_heterozyous allele

Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report
BRC No.: RBRC00858
Strain Name (Official Nomenclature): B6;129S6-Irf3<tm1Ttg>
Strain of Origin: Mutant Stock
Strain of origin: 129S6/SvEv
Donor strain: EK.CCE (ES cell)
Background strain: C57BL/6J and 129 mixed
Date: 2010/1/13
ID of animals surveyed
S00330204 S00330209(N8?+F27) (N8?+F27)(Generation1) / Sex
Locus
Type Chr Location2
FemaleMale
1 D1Mit212
SSLP 1 Chr1:40049840-40049985
b/bb/b
2 D1Mit306
SSLP 1 Chr1:98827535-98827643
b/bb/b
3 D2Mit433
SSLP 2 Chr2:57149522-57149694
b/bb/b
4 D3Mit333
SSLP 3 Chr3:44511652-44511774
b/bb/b
5 D3Mit106
SSLP 3 Chr3:111841219-111841382
b/bb/b
6 D4Mit149
SSLP 4 Chr4:3584271-3584381
b/bb/b
7 D4Mit108
SSLP 4 Chr4:38319122-38319254
b/bb/b
8 D5Mit148
SSLP 5 Chr5:32278263-32278411
b/bb/b
9 D5Mit168
SSLP 5 Chr5:137534245-137534394
b/bb/b
10 D6Mit9
SSLP 6 Chr6:87375892-87376028
b/bb/b
11 D7Mit230
SSLP 7 Chr7:56764849-56764953
b/bb/b
12 D8Mit293
SSLP 8 Chr8:37226054-37226173
b/bb/b
13 D9Mit191
SSLP 9 Chr9:46494456-46494601
b/bb/b
14 D10Mit282 SSLP 10 Chr10:20654078-20654202
b/bb/b
15 D11Mit219 SSLP 11 Chr11:72132472-72132603
b/bb/b
16 D12Mit190 SSLP 12 Chr12:60829453-60829579
b/bb/b
17 D13Mit179 SSLP 13 Chr13:44860362-44860453
b/bb/b
18 D14Mit50
SSLP 14 Chr14:21831604-21831793
b/bb/b
19 D15Mit138 SSLP 15 Chr15:39861164-39861310
b/bb/b
20 D16Mit50
SSLP 16 Chr16:78953071-78953200
b/bb/b
21 D17Mit152 SSLP 17 Chr17:65689824-65689949
b/bb/b
22 D18Mit68
SSLP 18 Chr18:21594126-21594238
b/bb/b
23 D19Mit16
SSLP 19 Chr19:20420342-20420474
b/bb/b
24 DXMit117
SSLP X Chr X:127822562-127822687
b/bb/bNo.： 100113-18

a-typeb-type (B6 type)
C3,FVB
B6,D2,129,BALB,CBA
D2,129
B6,BALB,C3,CBA,FVB
D2,C3,129,CBA,FVB
B6,BALB
C3,CBA,FVB
B6,D2,BALB,129
D2,BALB,C1,129,CBA,FVB
B6
D2,BALB,C3,129,CBA
B6,FVB
BALB,C3,129
B6,D2,CBA,FVB
BALB,C3,129,CBA,FVB
B6,D2
D2,BALB,C3,129,CBA,FVB
B6
D2,C3,129,CBA,FVB
B6,BALB
D2,C3,129,FVB
B6,BALB,CBA
D2,BALB,C3,129,CBA,FVB
B6
D2,BALB,C3,CBA,FVB
B6,129
BALB,129,FVB
B6,D2,C3,CBA
D2,129
B6,BALB,C3,CBA,FVB
D2,FVB
B6,BALB,C3,129,CBA
BALB,C3,129,CBA,FVB
B6,D2
D2,BALB,129,CBA,FVB
B6,C3
C3
B6,D2,BALB,129,CBA,FVB
D2,129,CBA,FVB
B6,BALB,C3
D2,BALB,FVB
B6,C3,129,CBA
D2,BALB,CBA,FVB
B6,C3,129
D2,C3,CBA
B6,BALB,FVB
D2,129,FVB
B6,BALB,C3,CBA

1

Generation definitions: N_Number of backcross generations; F_Filial or inbreeding generations; M_Cross-intercross breeding generations; ?: Use when the priorbreeding history is not known; +_Generation numbers before the "+" took place in the donating investigator, after the "+", at RIKEN BRC.
2
based on UniSTS annotation of NCBI Build 37
Haplotype combination:a/a_a-type homozygous allele, b/b_b-type homozygous allele, a/b_heterozyous allele

Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report

No.： -

BRC No.: RBRC00858
Strain Name (Official Nomenclature): B6;129S6-Irf3<tm1Ttg>
Strain of Origin: Mutant Stock
Strain of origin: 129S6/SvEv
Donor strain: EK.CCE (ES cell)
Background strain: C57BL/6J and 129 mixed
Date: ID of animals surveyed#3#5
1(N8?+F15) (N8?+F15)(Generation ) / Sex
Locus
Type Location2
FemaleMale
SSLP Chr1:19802051-19802209
1 D1Mit3
B6/B6
B6/B6
SSLP Chr1:Syntenic
2 D1Mit322
B6/B6
B6/B6
SSLP Chr1:73734337-73734492
3 D1Mit19
B6/B6
B6/B6
SSLP Chr1:98827535-98827643
4 D1Mit306
B6/B6
B6/B6
SSLP Chr1:154473511-154473632
5 D1Mit501
B6/B6
B6/B6
SSLP Chr1:176558864-176559001
6 D1Mit150
B6/B6
B6/B6
SSLP Chr2:27277814-27277911
7 D2Mit431
129/129
129/129
SSLP Chr2:65277459-65277647
8 D2Mit9
B6/B6
B6/B6
SSLP Chr2:106750096-106750239
9 D2Mit206
129/129
129/129
SSLP Chr2:153798642-153798751
10 D2Mit493
B6/B6
B6/B6
SSLP Chr2:168761877-168761999
11 D2Mit456
B6/B6
B6/B6
SSLP Chr2:178535250-178535364
12 D2Mit148
B6/B6
B6/B6
SSLP Chr3:31137265-31137413
13 D3Mit151
129/129
129/129
SSLP Chr3:71816030-71816141
14 D3Mit278
129/129
129/129
SSLP Chr3:123334565-123334712
15 D3Mit318
B6/B6
B6/B6
SSLP Chr3:148932688-148932790
16 D3Mit293
B6/B6
B6/B6
SSLP Chr4:3584271-3584381
17 D4Mit149
B6/B6
B6/B6
SSLP Chr4:25610387-25610576
18 D4Mit2
B6/B6
B6/B6
SSLP
Chr4:63026328-63026475
19 D4Mit17
129/129
129/129
SSLP Chr4:66843059-66843205
20 D4Mit178
129/129
129/129
SSLP Chr4:123838543-123838630
21 D4Mit308
B6/B6
B6/B6
SSLP Chr4:153093974-153094108
22 D4Mit254
B6/B6
B6/B6
SSLP Chr5:8797672-8797875
23 D5Mit48
B6/B6
B6/B6
SSLP Chr5:Syntenic
24 D5Mit267
B6/B6
B6/B6
SSLP Chr5:89700063-89700172
25 D5Mit398
129/129
129/129
SSLP Chr5:125309605-125309718
26 D5Mit95
B6/B6
B6/B6
SSLP Chr5:127286127-127286250
27 D5Mit320
B6/B6
B6/B6
SSLP Chr5:139608988-139609104
28 D5Mit374
B6/B6
B6/B6
SSLP Chr6:55505943-55506037
29 D6Mit316
B6/B6
B6/B6
SSLP Chr6:87375892-87376028
30 D6Mit9
B6/B6
B6/B6
SSLP Chr6:93413943-93414087
31 D6Mit102
B6/B6
B6/B6
SSLP Chr6:114232092-114232201
32 D6Mit55
B6/B6
B6/B6
SSLP Chr6:136372097-136372213
33 D6Mit339
B6/B6
B6/B6
SSLP Chr7:31118079-31118215
34 D7Mit246
129/129
129/129
SSLP Chr7:52767912-52768056
35 D7Mit81
129/129
129/129
SSLP Chr7:126237672-126237821
36 D7Mit238
129/129
129/129
SSLP Chr7:Syntenic
37 D7Mit189
129/129
129/129
Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report

No.： -

BRC No.: RBRC00858
Strain Name (Official Nomenclature): B6;129S6-Irf3<tm1Ttg>
Strain of Origin: Mutant Stock
Strain of origin: 129S6/SvEv
Donor strain: EK.CCE (ES cell)
Background strain: C57BL/6J and 129 mixed
Date: ID of animals surveyed#3#5
1(N8?+F15) (N8?+F15)(Generation ) / Sex
Locus
Type Location2
FemaleMale
SSLP Chr8:37226054-37226173
38 D8Mit293
B6/B6
B6/B6
SSLP Chr8:91727514-91727649
39 D8Mit81
B6/B6
B6/B6
SSLP Chr8:Syntenic
40 D8Mit14
B6/B6
B6/B6
SSLP Chr9:41695932-41696113
41 D9Mit328
B6/B6
B6/B6
SSLP Chr9:87838840-87839011
42 D9Mit269
SSLP
Chr9:115307830-115307967
43 D9Mit214
129/129
129/12944 D10Mit282 SSLP Chr10:20654078-20654202
B6/B6
B6/B6
45 D10Mit223 SSLP Chr10:67674940-67675089
B6/B6
B6/B6
SSLP Chr10:91885360-91885556
46 D10Mit11
B6/B6
B6/B6
47 D10Mit180 SSLP Chr10:117587667-117587800
B6/B6
B6/B6
48 D11Mit236 SSLP Chr11:45088548-45088651
49 D11Mit260 SSLP Chr11:61609496-61609590
50 D11Mit219 SSLP Chr11:72132472-72132603
B6/B6
B6/B6
51 D11Mit333 SSLP Chr11:108575096-108575220
129/129
129/12952 D12Mit153 SSLP Chr12:35920169-35920310
SSLP Chr12:70900839-70901009
53 D12Mit34
129/129
129/129
54 D12Mit102 SSLP Chr12:105536654-105536786
B6/B6
B6/B6
SSLP Chr12:Syntenic
55 D12Mit7
B6/B6
B6/B6
56 D13Mit117 SSLP Chr13:37654832-37654949
B6/B6
B6/B6
57 D13Mit179 SSLP Chr13:44860362-44860453
B6/B6
B6/B6
58 D13Mit147 SSLP Chr13:98359080-98359187
B6/B6
B6/B6
SSLP Chr13:120128451-120128643
59 D13Mit35
129/129
129/12960 D14Mit259 SSLP Chr14:54729500-54729624
129/129
129/129
61 D14Mit193 SSLP Chr14:71921427-71921544
129/129
129/12962 D14Mit165 SSLP Chr14:106982674-106982807
B6/B6
B6/B6
63 D15Mit136 SSLP Chr15:30776615-30776757
B6/B6
B6/B6
SSLP Chr15:43279930-43280030
64 D15Mit5
B6/B6
B6/B6
SSLP Chr15:74075586-74075734
65 D15Mit29
B6/B6
B6/B6
SSLP Chr15:83586169-83586316
66 D15Mit33
B6/B6
B6/B6
67 D16Mit131 SSLP Chr16:7319135-7319274
B6/B6
B6/B6
SSLP Chr16:38433970-38434154
68 D16Mit59
B6/B6
B6/B6
SSLP Chr16:78953071-78953200
69 D16Mit50
129/129
129/12970 D16Mit106 SSLP Chr16:98051757-98051902
B6/B6
B6/B6
71 D17Mit213 SSLP Chr17:16752157-16752280
B6/B6
B6/B6
72 D17Mit108 SSLP Chr17:49744546-49744658
B6/B6
B6/B6
73 D17Mit187 SSLP Chr17:79631462-79631708
129/129
129/129
74 D18Mit177 SSLP Chr18:41142161-41142331
B6/B6
B6/B6
Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report

No.： -

BRC No.: RBRC00858
Strain Name (Official Nomenclature): B6;129S6-Irf3<tm1Ttg>
Strain of Origin: Mutant Stock
Strain of origin: 129S6/SvEv
Donor strain: EK.CCE (ES cell)
Background strain: C57BL/6J and 129 mixed
Date: ID of animals surveyed#3#5
1(N8?+F15) (N8?+F15)(Generation ) / Sex
Locus
Type Location2
FemaleMale
75 D18Mit184 SSLP Chr18:66978897-66979068
B6/B6
B6/B6
76 D18Mit142 SSLP Chr18:75548607-75548726
B6/B6
B6/B6
77 D18Mit216 SSLP Chr18:83585146-83585269
B6/B6
B6/B6
SSLP Chr19:21916083-21916201
78 D19Mit96
B6/B6
B6/B6
SSLP Chr19:37331405-37331552
79 D19Mit88
129/129
129/129
D19Mit90
SSLP
Chr19:42295742-42295862
80
129/129
129/129
SSLP Chr19:59674330-59674465
81 D19Mit71
B6/B6
B6/B6
82 DXMit166 SSLP ChrX:49036675-49036788
B6/B6
B6/B6
83 DXMit210 SSLP ChrX:69741824-69741934
B6/B6
B6/B6
84 DXMit130 SSLP ChrX:130298712-130298880
1

Generation definitions: N_Number of backcross generations; F_Filial or inbreeding generations;
M_Cross-intercross breeding generations; ?: Use when the prior breeding history is not known;+_Generation numbers before the "+" took place in the donating investigator, after the "+", at RIKEN BRC.
2
based on UniSTS annotation of NCBI Build 37
Haplotype combination:
B6/B6_C57BL/6 homozygous allele, 129/129_129 homozygous allele, B6/129_heterozyous allele

Kazuyuki Mekada, Ph.D. (2011/12/5)