Genetic Background Monitoring Report

No.： 120313-30

BRC No.: RBRC02258
Strain Name (Official Nomenclature): B6;129S(Cg)-Grin2c<tm1Nak>
Strain Type: Targeted (Knock-out)
Strain of Origin: 129S/SvEv-Gpi1<c>
Donor Strain: CCE/EK.CCE (ES cell)
Background Strain: C57BL/6, 129S and ? mixed
Date: 2012/6/19
ID of animals surveyed
080905C03
1(G?+F2)(Generation ) / SexLocus
Type Location2
Male
SSLP Chr1:19802051-19802209
B6/B6
1 D1Mit3
SSLP Chr1:Syntenic
B6/1292 D1Mit322
SSLP Chr1:73734337-73734492
B6?/B6?
3 D1Mit19
SSLP Chr1:98827535-98827643
B6/1294 D1Mit306
SSLP Chr1:154473511-154473632
B6/1295 D1Mit501
SSLP Chr1:176558864-176559001
B6/B6
6 D1Mit150
SSLP Chr2:27277814-27277911
B6/B6
7 D2Mit431
SSLP Chr2:65277459-65277647
B6?/B6?
8 D2Mit9
SSLP Chr2:106750096-106750239
B6?/B6?
9 D2Mit206
SSLP Chr2:153798642-153798751
B6/12910 D2Mit493
SSLP Chr2:168761877-168761999
129/129
11 D2Mit456
SSLP Chr2:178535250-178535364?/?
12 D2Mit148
SSLP Chr3:31137265-31137413
129/129
13 D3Mit151
SSLP Chr3:71816030-71816141?/?
14 D3Mit278
SSLP Chr3:123334565-123334712
B6/B6
15 D3Mit318
SSLP Chr3:148932688-148932790
129/129
16 D3Mit293
SSLP Chr4:3584271-3584381
129/129
17 D4Mit149
SSLP Chr4:25610387-25610576
B6/B6
18 D4Mit2
SSLP Chr4:63026328-63026475
B6/B6
19 D4Mit17
SSLP Chr4:66843059-66843205
B6/B6
20 D4Mit178
SSLP Chr4:123838543-123838630
B6/B6
21 D4Mit308
SSLP Chr4:153093974-153094108
B6/12922 D4Mit254
SSLP Chr5:8797672-8797875
B6/B6
23 D5Mit48
SSLP Chr5:Syntenic?/?
24 D5Mit267
SSLP Chr5:89700063-89700172
B6/12925 D5Mit398
SSLP Chr5:125309605-125309718
B6/12926 D5Mit95
SSLP Chr5:127286127-127286250
B6/12927 D5Mit320
SSLP Chr5:139608988-139609104
B6/12928 D5Mit374
SSLP Chr6:55505943-55506037?/?
29 D6Mit316
SSLP Chr6:87375892-87376028
129/12930 D6Mit9
SSLP Chr6:93413943-93414087?/?
31 D6Mit102
SSLP Chr6:114232092-114232201
129/129
32 D6Mit55
SSLP Chr6:136372097-136372213?/?
33 D6Mit339
SSLP Chr7:31118079-31118215
B6/B6
34 D7Mit246
SSLP Chr7:52767912-52768056
B6/B6
35 D7Mit81
SSLP Chr7:126237672-126237821?/?
36 D7Mit238
Kazuyuki Mekada, Ph.D. (2012/12/20)

Genetic Background Monitoring Report

No.： 120313-30

BRC No.: RBRC02258
Strain Name (Official Nomenclature): B6;129S(Cg)-Grin2c<tm1Nak>
Strain Type: Targeted (Knock-out)
Strain of Origin: 129S/SvEv-Gpi1<c>
Donor Strain: CCE/EK.CCE (ES cell)
Background Strain: C57BL/6, 129S and ? mixed
Date: 2012/6/19
ID of animals surveyed
080905C03
1(G?+F2)(Generation ) / SexLocus
Type Location2
Male
SSLP Chr7:Syntenic
129/129
37 D7Mit189
SSLP Chr8:37226054-37226173
B6/B6
38 D8Mit293
SSLP Chr8:91727514-91727649
B6/B6
39 D8Mit81
SSLP Chr8:Syntenic
B6/B6
40 D8Mit14
SSLP Chr9:41695932-41696113
B6/12941 D9Mit328
SSLP Chr9:87838840-87839011
129/129
42 D9Mit269
SSLP Chr9:115307830-115307967
B6/B6
43 D9Mit214
B6/B6
44 D10Mit282 SSLP Chr10:20654078-20654202
B6/B6
45 D10Mit223 SSLP Chr10:67674940-67675089
SSLP Chr10:91885360-91885556
129/12946 D10Mit11?/?
47 D10Mit180 SSLP Chr10:117587667-117587800
B6?/B6?
48 D11Mit236 SSLP Chr11:45088548-45088651?/?
49 D11Mit260 SSLP Chr11:61609496-61609590
129/12950 D11Mit219 SSLP Chr11:72132472-72132603?/?
51 D11Mit333 SSLP Chr11:108575096-108575220
B6/12952 D12Mit153 SSLP Chr12:35920169-35920310
SSLP Chr12:70900839-70901009
B6/B6
53 D12Mit34
129/129
54 D12Mit102 SSLP Chr12:105536654-105536786
SSLP Chr12:Syntenic
129/129
55 D12Mit7
B6/B6
56 D13Mit117 SSLP Chr13:37654832-37654949
B6/12957 D13Mit179 SSLP Chr13:44860362-44860453
129/12958 D13Mit147 SSLP Chr13:98359080-98359187
SSLP Chr13:120128451-120128643
B6/B659 D13Mit35?/?
60 D14Mit259 SSLP Chr14:54729500-54729624
129/129
61 D14Mit193 SSLP Chr14:71921427-71921544?/?
62 D14Mit165 SSLP Chr14:106982674-106982807
B6/B6
63 D15Mit136 SSLP Chr15:30776615-30776757
SSLP Chr15:43279930-43280030?/?
64 D15Mit5
SSLP Chr15:74075586-74075734?/?
65 D15Mit29
SSLP Chr15:83586169-83586316
129/129
66 D15Mit33
B6/B6
67 D16Mit131 SSLP Chr16:7319135-7319274
SSLP Chr16:38433970-38434154
B6/B6
68 D16Mit59
SSLP Chr16:78953071-78953200
B6/B6
69 D16Mit50
B6/12970 D16Mit106 SSLP Chr16:98051757-98051902
B6/B6
71 D17Mit213 SSLP Chr17:16752157-16752280
B6/12972 D17Mit108 SSLP Chr17:49744546-49744658
Kazuyuki Mekada, Ph.D. (2012/12/20)

Genetic Background Monitoring Report

No.： 120313-30

BRC No.: RBRC02258
Strain Name (Official Nomenclature): B6;129S(Cg)-Grin2c<tm1Nak>
Strain Type: Targeted (Knock-out)
Strain of Origin: 129S/SvEv-Gpi1<c>
Donor Strain: CCE/EK.CCE (ES cell)
Background Strain: C57BL/6, 129S and ? mixed
Date: 2012/6/19
ID of animals surveyed
080905C03
1(G?+F2)(Generation ) / SexLocus
Type Location2Male
B6/12973 D17Mit187 SSLP Chr17:79631462-79631708
129/129
74 D18Mit177 SSLP Chr18:41142161-41142331
129/12975 D18Mit184 SSLP Chr18:66978897-66979068
129/129
76 D18Mit142 SSLP Chr18:75548607-75548726
B6/12977 D18Mit216 SSLP Chr18:83585146-83585269
SSLP Chr19:21916083-21916201
129/12978 D19Mit96
SSLP Chr19:37331405-37331552B6/129
79 D19Mit88
SSLP Chr19:42295742-42295862
129/12980 D19Mit90
SSLP Chr19:59674330-59674465
B6/12981 D19Mit71
129/129
82 DXMit166 SSLP ChrX:49036675-49036788
B6/B6
83 DXMit210 SSLP ChrX:69741824-69741934
B6/B6
84 DXMit130 SSLP ChrX:130298712-130298880
1
Generation definitions: N_Number of backcross generations; F_Filial or inbreeding generations;
M_Cross-intercross breeding generations; ?: Use when the prior breeding history is not known;+_Generation numbers before the "+" took place in the donating investigator, after the "+", at RIKEN
BRC.
2
based on UniSTS annotation of NCBI Build 37
Haplotype combination:
B6/B6_C57BL/6 homozygous allele, 129/129_129 homozygous allele, B6/129_heterozyous allele

Kazuyuki Mekada, Ph.D. (2012/12/20)