Genetic Background Monitoring Report

No.： 100205-12

BRC No.: RBRC01361
Strain Name (Official Nomenclature): B6.B6CB-Trp53<tm1Sia>
Strain Type: Mutant Strain
Strain of origin: (C57BL/6 x CBA)F1
Donor strain: TT2 (ES cell)
Background strain: C57BL/6NCrSlc
Date: 2010/2/5
ID of animals surveyed
0000201 (C)1(N20<+N0)(Generation ) / SexLocus
Type Location2
Male
SSLP Chr1:33844238-33844362
B6/B6
1 D1Mit318
SSLP Chr1:70413813-70414004
B6/B6
2 D1Mit251
SSLP Chr1:92584545-92584683
B6/B6
3 D1Mit10
SSLP Chr1:162387226-162387364
B6/B6
4 D1Mit105
SSLP Chr1:187046266-187046383
B6/B6
5 D1Mit221
SSLP Chr2:40654653-40654777
B6/B6
6 D2Mit369
SSLP Chr2:114283330-114283458
B6/B6
7 D2Mit274
SSLP Chr2:153798642-153798751
B6/B6
8 D2Mit493
SSLP Chr2:162180765-162180901
B6/B6
9 D2Mit263
SSLP Chr3:7521114-7521248
B6/B6
10 D3Mit164
SSLP Chr3:44511652-44511774
B6/B6
11 D3Mit333
SSLP Chr3:89036582-89036709
B6/B6
12 D3Mit49
SSLP Chr3:123334565-123334712
B6/B6
13 D3Mit318
SSLP Chr3:147806703-147806850
B6/B6
14 D3Mit45
SSLP Chr4:3584271-3584381
B6/B6
15 D4Mit149
SSLP Chr4:53563711-53563839
B6/B6
16 D4Mit111
SSLP Chr4:93541561-93541759
B6/B6
17 D4Mit166
SSLP Chr4:132983282-132983386
B6/B6
18 D4Mit204
SSLP Chr4:149966385-149966510
B6/B6
19 D4Mit33
SSLP Chr5:32278263-32278411
B6/B6
20 D5Mit148
SSLP Chr5:54734074-54734215
B6/B6
21 D5Mit394
SSLP Chr5:89700042-89700177
B6/B6
22 D5Mit259
SSLP Chr5:125309605-125309718
B6/B6
23 D5Mit95
SSLP Chr5:137534245-137534394
B6/B6
24 D5Mit168
SSLP Chr6:48676555-48676704
B6/B6
25 D6Mit74
SSLP Chr6:87375892-87376028
B6/B6
26 D6Mit9
SSLP Chr6:125306664-125306803
B6/B6
27 D6Mit254
SSLP Chr6:147000978-147001083
B6/B6
28 D6Mit373
SSLP Chr7:47279833-47279979
B6/B629 D7Mit228
SSLP Chr7:86521272-86521399
B6/B6
30 D7Mit250
SSLP Chr7:111543239-111543373
B6/B6
31 D7Mit220
SSLP Chr7:143722527-143722723
B6/B6
32 D7Mit12
SSLP Chr8:37226054-37226173
B6/B6
33 D8Mit293
SSLP Chr8:95209247-95209396
B6/B6
34 D8Mit248
SSLP Chr8:116884022-116884117
B6/B6
35 D8Mit271
SSLP Chr8:131419404-131419542
B6/B6
36 D8Mit156
Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report

No.： 100205-12

BRC No.: RBRC01361
Strain Name (Official Nomenclature): B6.B6CB-Trp53<tm1Sia>
Strain Type: Mutant Strain
Strain of origin: (C57BL/6 x CBA)F1
Donor strain: TT2 (ES cell)
Background strain: C57BL/6NCrSlc
Date: 2010/2/5
ID of animals surveyed
0000201 (C)1(N20<+N0)(Generation ) / Sex
Locus
Type Location2
Male
SSLP Chr9:41695932-41696113
B6/B6
37 D9Mit328
SSLP Chr9:60421238-60421384
B6/B6
38 D9Mit207
SSLP Chr9:101431148-101431251
B6/B6
39 D9Mit182
SSLP Chr9:120198563-120198742
B6/B6
40 D9Mit18
B6/B6
41 D10Mit123 SSLP Chr10:9952319-9952461
SSLP Chr10:28871992-28872234
B6/B6
42 D10Mit3
SSLP Chr10:82117849-82118032
B6/B6
43 D10Mit42
B6/B6
44 D10Mit180 SSLP Chr10:117587667-117587800
B6/B6
45 D11Mit227 SSLP Chr11:17177055-17177235
B6/B6
46 D11Mit242 SSLP Chr11:63242083-63242198
B6/B6
47 D11Mit212 SSLP Chr11:88670076-88670216
B6/B648 D11Mit333 SSLP Chr11:108575096-108575220
B6/B6
49 D12Mit136 SSLP Chr12:30858603-30858749
SSLP Chr12:70900839-70901009
B6/B6
50 D12Mit34
B6/B6
51 D12Mit158 SSLP Chr12:83720003-83720152
SSLP Chr12:Syntenic
B6/B6
52 D12Mit7
SSLP Chr13:20523211-20523371
B6/B6
53 D13Mit3
B6/B6
54 D13Mit250 SSLP Chr13:56424004-56424131
B6/B6
55 D13Mit213 SSLP Chr13:109037352-109037500
SSLP Chr13:120128451-120128643
B6/B6
56 D13Mit35
B6/B6
57 D14Mit141 SSLP Chr14:47379212-47379351
B6/B6
58 D14Mit203 SSLP Chr14:Syntenic
B6/B6
59 D14Mit193 SSLP Chr14:71921427-71921544
B6/B6
60 D14Mit165 SSLP Chr14:106982674-106982807
B6/B6
61 D15Mit175 SSLP Chr15:9202691-9202864
SSLP Chr15:43279930-43280030
B6/B6
62 D15Mit5
B6/B6
63 D15Mit156 SSLP Chr15:71155976-71156119
B6/B6
64 D15Mit246 SSLP Chr15:101996392-101996510
B6/B6
65 D16Mit131 SSLP Chr16:7319135-7319274
SSLP Chr16:38433970-38434154
B6/B6
66 D16Mit59
SSLP Chr16:78953071-78953200
B6/B667 D16Mit50
B6/B6
68 D16Mit106 SSLP Chr16:98051757-98051902
B6/B6
69 D17Mit164 SSLP Chr17:3924615-3924747
SSLP Chr17:43641789-43641943
B6/B6
70 D17Mit52
SSLP Chr17:74149996-74150148
B6/B6
71 D17Mit93
B6/B6
72 D17Mit123 SSLP Chr17:93598959-93599091
Kazuyuki Mekada, Ph.D. (2011/12/5)

Genetic Background Monitoring Report

No.： 100205-12

BRC No.: RBRC01361
Strain Name (Official Nomenclature): B6.B6CB-Trp53<tm1Sia>
Strain Type: Mutant Strain
Strain of origin: (C57BL/6 x CBA)F1Donor strain: TT2 (ES cell)
Background strain: C57BL/6NCrSlc
Date: 2010/2/5
ID of animals surveyed
0000201 (C)
1(N20<+N0)(Generation ) / SexLocus
Type Location2
Male
SSLP Chr18:32648763-32648967
B6/B6
73 D18Mit60
SSLP Chr18:61299030-61299225
B6/B6
74 D18Mit51
SSLP Chr18:77065228-77065337
B6/B6
75 D18Mit80
SSLP Chr19:20420342-20420474
B6/B676 D19Mit16
SSLP Chr19:32713513-32713760
B6/B677 D19Mit13
SSLP Chr19:54969040-54969161
B6/B678 D19Mit1
B6/B679 DXMit172 SSLP ChrX:119197077-119197224
B6/B680 DXMit130 SSLP ChrX:130298712-130298880
1
Generation definitions: N_Number of backcross generations; F_Filial or inbreeding generations;
M_Cross-intercross breeding generations; ?: Use when the prior breeding history is not known;+_Generation numbers before the "+" took place in the donating investigator, after the "+", at RIKEN
BRC.
2
based on UniSTS annotation of NCBI Build 37
Haplotype combination:
B6/B6_C57BL/6 homozygous allele, CBA/CBA_CBA homozygous allele, B6/CBA_heterozyous allele

Kazuyuki Mekada, Ph.D. (2011/12/5)