MCE USA
Tel:609-228-6898

Email: tech@MedChemExpress.com

Compound Handling Instructions

1

What are the shipping conditions of MCE products?

65

How can I prepare the compound stock solution?

A: MCE stable products are shipped at room temperature.

Select the appropriate solvent for the preparation of stock solution based on your

B: Liquid or special products are shipped with blue ice or MCE® Cooling Rack in aexperiment needs.

foam holding box. MCE products are relatively stable at room temperature. TheirSolubility information is available at the product webpage. Currently we only offerquality will not be affected if the blue ice melts upon receiving, and products cansolubility data in DMSO and/or water, for solubility of other solvents, please email

be used with confidence.

to tech@MedChemExpress.com. If you can not find the solubility information

MCE is dedicated to provide high quality products and meet customer

you are looking for, or you are having difficulty preparing a product in solution

satisfaction. If you have any questions or concerns, please contact after-sales

form, you can also get help via the email above. Once prepared, aliquot the stock

staff on phone 609-228-6898 or by email sales@MedChemExpress.com.

solution to routine usage volumes and store at -20°C or -80°C. Avoid repeated

2How can I obtain product Data Sheet, QA ﬁles, etc.?

A: We automatically send product Data Sheet and QA documents to your email
address after shipment.

Samplefreeze and thaw cycles.

B: You can download these files on the product webpage of our website
MedChemExpress.com.
C: You can also request the materials from our customer service.

3

What are the proper storage guidelines for MCE product?

Recommended storage conditions and precautions regarding proper producthandling are contained in the product Certificate of Analysis (COA).
Here are GENERAL storage guidelines for compounds:
Powder:In solvent:DMSO > A mg/mL

DMSO soluble (A mg can be dissolved in 1 mL
DMSO, saturation unknown)

H2O < B mg/mL
DMSO < C mg/mL

H2O insoluble or slightly soluble
DMSO insoluble or slightly soluble (C mg can not
be dissolved in 1 mL DMSO)

Molarity Calculator from MCE official website is recommended for thecalculation: www.MedChemExpress.com/Molarity%20Calculator.htm

6How do I dilute the compound solution for cell assay?

Stock solution using ddH2O as a solvent can be directly diluted with medium toprepare the working solution.
When DMSO is used to prepare the stock solution, the stock solution is diluted in-20°C

3 years

the culture medium to prepare a working solution. Make sure the concentration

4°C

2 yearsof DMSO is <0.5% to avoid poisoning the cells. A negative control in the-80°C

6 months

experiment is usually the culture medium with DMSO at the same concentration.

-20°C

1 monthIt is recommended that the process of dilution is performed in a stepwise

If the solution is stored at -20°C for more than one month, it should be
re-examined to ensure its efficacy. Avoid repeated freeze and thaw cycles.
Storage conditions for some special products should refer to their COAs.

manner to avoid compound precipitating caused by fast change ofconcentration.

7How do I use these compounds during animal experiments? ie:method of administration, dosage, solvent preparation, and4

How should I handle the products before formulation or use?

During transportation, the compounds may adhere to the neck or cap of the vial.
Before opening the vial, please centrifuge to gather the compound at the bottomof the vial.

administrative cycle?
Stock solution using ddH2O as a solvent can be directly diluted with PBS or 0.9%
NaCl to ready the working solution.
Stock solution using DMSO as a solvent can also be diluted with PBS or 0.9%
NaCl to prepare the working solution. In order to reduce its toxicity to animals, thefinal concentration of DMSO in working solution should preferably be 2% or lower.
When precipitates form during the dilution process due to their low watersolubility, you can also use a co-solvent to help dissolve the compounds.
CommonInhibitors, Screening Libraries, Proteinswww.MedChemExpress.com

co-solvents

containglycerol,

Tween

80,

sodiumcarboxymethylcellulose (CMC-Na), cyclodextrin, PEG400, etc. A suspension canalso be used for oral or intraperitoneal injection. Please send an email totech@MedChemExpress.com if you require further assistance.

Methods of administration and solvent preparation used in publications may beAdministration volumes considered good practice (and possible maximal

available at the product webpage. MCE has not independently confirmed thedose volumes):

accuracy of these methods and they are distributed for reference only.
Route and volumes (mL/kg)
Conversion between different animal models based on BSA:
Species

Weight (kg)Body Surface Area (m2)

Km factor

Dog

100.5

20Rabbit

1.8

0.15

12

Guinea pig

0.4

0.05

8Rat

0.15

0.025

6Hamster

0.08

0.02

5

Mouse

0.02

0.007

3Animal A (mg/kg) = Animal B (mg/kg) multiplied bySpecies

Oral

s.c.

i.p.

i.m.

i.v. (bolus)

i.v. (slow inj.)

Mouse

10 (50)10 (40)20 (80)0.05 (0.1)

5(25)

Rat

10 (40)5 (10)

10 (20)0.1 (0.2)

5(20)Rabbit10 (15)1 (2)5 (20)

0.25 (0.5)

2(10)

Dog

5 (15)1 (2)

1 (20)

0.25 (0.5)

2.5(5)

68

Is the compound sterile?

DMSO itself is strongly bactericidal and will not introduce bacteria toAnimal B Km

compounds. It is however important to keep the operating environment and theAnimal A Km

instrument be sterilized before experimental use. Compounds can also besterilized by filtration prior to use depending on specific experimental
requirements. High temperature and high pressure sterilization are NOT
recommended.

Products are for research use only and are not intended for human use. We do not sell to patients.
Our Product Portfolio Includes:
Inhibitors

Screening Libraries

Proteins

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MedChemExpress (MCE) compound libraries consist of over 20,000+ bioactive molecules with validated biological and pharmacological activities.
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