International Collaboration in
Networking of Culture Collections
and Microbial Research
Katsuhiko ANDO
Biological Resource Center,
National Institute of Technology and Evaluation (NITE), JAPANSide Event of ICNP 3Feb. 2014 in Pyeongchang, Republic of Korea

1.Activity of ACM
2.Activity of NITE

1.Activity of ACM
2.Activity of NITEACM 1: Oct. 2004, Tsukuba, Japan

ACM 4: Nov. 2007, Chibinon, Indonesia

ACM 7: Oct. 2010, Kazusa, Japan

ACM 2: Nov. 2005, Bangkok, Thailand

ACM 5: Oct. 2008, Daejeon, Korea

ACM 8: Oct. 2011, KL, Malaysia

ACM 3: Nov. 2006, Beijing, China

ACM 6: Nov. 2009, Hanoi, Vietnam

ACM 9: Oct. 2012, Chang Mai, Thailand

ACM
ACM 10: Sep. 2013, Beijing, China

What is ACM?
• Asian Consortium for the Conservation andSustainable Use of Microbial Resources• ACM was established in 2004 byrepresentatives of 12 Asian countries on theoccasion of ICCC10 in Tsukuba, Japanto strengthen the relationships among Asiancountries, as well as encourage andfacilitate international cooperation forbiotechnology using microbiologicalresources in Asia.

ACM Members are 17 Institutions from 13 Countries- 11 Culture Collections Included -Institute of Biology
Mongolian Academy of Sciences-Institute of Microbiology
Chinese Academy of Sciences-Research Institute of Science-KRIBB-BRC-NAAS-KNRRC-IMTECH.
-Vietnam NationalUniv.
-Pathein Univ.

-MARDI

BIOTEC-Ministry ofEnvironment-LIPI-Univ. Philippines-Univ. St.Thomas-NITE-RIKEN-BRC

Aim of ACM• Framework for international cooperation to encourage
microbiological researches• Development of microbial resources characteristic foreach country• Construction of the mechanism for academia andindustry to utilize microbial resources• Establishment of BRC network• Establishment of international standards for biologicalmaterial transfer and benefit-sharing• Improvement and share of standardized techniquesTask Forces in ACM
1. Asian BRC Network (ABRCN)
Chair: Ken-ichiro Suzuki (Japan)

2. Human Resource Development (HRD)
Chair: Rosario G. Monsalud (Philippines)

3. Management of Material Transfer (MMT)
Co-chairs:

Katsuhiko Ando (Japan) andBubpha Techapattaraporn (Thailand)

The ACM members expect the expansion of the activities to reach theinternational standardized scheme to encourage microbiologists to study
biological diversity to solve the global problems on environments and humanwelfare.1. Task Force: Asian BRC Network(ABRCN)
Establishment of an On-Line Catalogue ofthe Member Collection•For the users to find the most convenient collection to obtainthe materials•To assist the establishment of strain database / on-line
catalogue to show the resources for small collections•To develop new functions for the traceability of the materials
in the network1. Task Force: Asian BRC Network (ABRCN)ACM Member Collections

TFBRC
ACM

IMCAS

International
BRC Community

Secretariat
BIOTEC

Maintenance
Combine

KRIBB

ACM
ABRCN
User Community

NBRC

5CC・・・

Assist development

Future Planz Data updating rulez Inviting other culture collectionsz Connection to the local networksz Link to the other databases

ABRCN: Integrated Microbial Strain Database

ABRCN website <http://www.abrcn.net>

2. Task Force: Human Resource
Development (HRD)The TF-HRD covers three objectives,1)Training,2)Exchange Scientists,3)Research collaboration addressing specific issues
to attract funding source.

“Training workshop on Long-Term
Preservation and Management of Microbial
Cultures with Agricultural Importance”
May 23-27, 2011
Hosted by BIOTEC, Thailand

Training Course Subjects
z Taxonomy
z Preservationz Informatics
z ManagementCooperation with:
ACM members, KRIBB, BIOTECH, NBRC,WDCM
Southeast Asian Regional Center for
Graduate Study and Research inAgriculture (SEARCA)
Participants: 30 of which 16 fromThailand and 14 from other ACM
member countries

2nd ACM Workshop (2013)“International Training Course on
Application of Biotechnology Tools
for Rapid Identification of Food-borne
Pathogens“
hosted by NBRC, Japan, supported by Southeast Asian Regional Center
for Graduate Study and Research in Agriculture (SEARCA)

Training Course Subjects
zRapid IdentificationzGenome informatics
zPCR
zMALDI/TOF MS identification
zFactory tourDomestic Workshops in Korea
ACM Microbial Taxonomy Training Course
The 3rd course will start on Sept. 30 2013 for 6 weeks
Human Resource Development Program for ACM members“Chemotaxonomy and phylogenetic analysis of novel
microorganisms”
Participants: 6 people from 6 countries
Period: 6 weeks

1st Week: Introduction, phylogenetic tree, cultivation
2nd Week: Fatty acids, polar lipid
3rd Week: Quinone, growth characteristics
4rd Week: Cell wall sugar
5th Week: DNA G+C mol%, DNA-DNA hybridization
6th Week: Presentation of results, discussion

Domestic Workshops in Indonesia• “Workshop on Molecular Identification of Lactic Acid Bacteria” Æ LIPI-Gadjah Mada Univ-NBRC Æ 23-24 January 2013•••••

Æ 30 participants
Workshop on “The Role of National Culture Collection in Facilitating Sustainable Development of Tropical Bioresources Under
Green Economy Agenda “Æ 1 March 2013 Æ 50 audiences
Preparing Documents for ISO 9001-2008 Management Systems in InaCC Æ April, September 2013 Æ 20 participants
How to operate MALDI-TOF Æ April 2013
How to operate GC-MS and HPLC Æ May 2013
How to operate L-drying machine Æ July 2013

Domestic Workshops in Mongolia
Training workshop on conventional methods of identification“Identification of Mitosporic Fungi”– August 10-11, 2013- 25 participants

Domestic Workshops in Vietnam• VTCC and NITE conducted 1 workshop (3 days) on“DNA-DNA hybridization”• Diversity of yeast (2 months)• Diversity of filamentous fungi (2 months)• VTCC and Thai Biotech conducted 1 workshop (3days) on Culture Collection Data management.

Domestic Workshops in Philippine• “Molecular Techniques for Microbial Identification”- November 22-23, 2012
- Trainers: Dr. Koji Mori & Dr. Takahide Ishida (NBRC)- 34 participants

3. Task Force: Management ofMaterial Transfer (MMT) The access and benefit-sharing (ABS) of the CBD (Article 15) and theNagoya Protocol are of special importance for Microbial Resource
Centers (MRCs) .
 In this TF, much work was done by MRCs towards the development of amodel for voluntary implementation of the CBD’s ABS and NP
provisions.

MMT Taskforce aims to develop a practicalguide for the management of material transfer.

MMT Taskforce Members
Co-Chair System

Chairpersons:
Ms. Bubpha Techapattaraporn(Thailand)
Dr. Katsuhiko Ando (Japan)
New members of MMT Taskforce

Ms. Rie Funabiki (Japan)
NITE

Dr. Tae-Eun Jin (Korea)
KRIBB

Dr. Linhuan Wu (China)
EDCM/IMCASConclusion
After the Nagoya Protocol enters into force, it
could be a hard work for MRCs and USERs touse overseas microbes preserved in MRCs.

We should develop streamlined way using
overseas microbes preserved in the MRCs for
MRCs and USERs under the Nagoya Protocol.

Network of International Exchange ofMicrobes in Asia under ACM(NIEMA)

 Initiated by pilot projects at the MMT-TF in ACM, a “Network of International
Exchange of Microbes in Asia (NIEMA)” has been discussed.

1.Activity of ACM
2.Activity of NITE

Joint Research Project with Asian countries

NITE
MOU

China
2005～Thailand
2012～

MOU

Countries (BRCs) conducting
cooperative research under MOU or PA

MOU between National Center for Genetic
Engineering and Biotechnology (BIOTEC),
National Science and Technology Development
Agency of Thailand and National Institute ofTechnology and Evaluation (NITE) of Japanon Conservation and Sustainable
Use of Microbial Resource

MOU between Institute of Microbiology of the Chinese Academy of Science (IM-CAS)
and National Institute of Technology and Evaluation (NITE) of Japan on Joint Program onthe Conservation and Sustainable Use of Biological Resources

Joint Research Project with Asian countries
MOU

Mongolia

Mongolia

P A

2006～

NITE
MyanmarVietnam
MOU

P A

Myanmar2004～2006,
2013～

Vietnam

MOU
P A

2004～
Countries continuing the Joint
Research Program for theConservation and Sustainable Use ofMicrobial Resources under MOU andPA

Indonesia
Indonesia

MOU
P A

2003～2009

NITE Scheme of Access and Benefit-sharing
Japan

ProviderCountryＮＩＴＥ

MOU as PIC

Government
Body

BiologicalResourceCenter

ＰＡ as MATResearchInstituteThe conservation of biological diversity

Microbes
The sustainable use of its components

Benefit-sharing(Technology transfer, capacity building)）

Users

The fair and equitable sharing of the benefits
arising out of the utilization of genetic resources

Users

With Indonesia

With Mongolia

With Vietnam

With Myanmar

with Indonesian Researcherswith Vietnam Researchers

August 2003

with Mongol Researchers

July 2007

March 2004

with Myanmar Researchers

May 2004

Workflow of the Project
Sampling

Primary Identification

Work together !!

Sample Treatment

Isolation

Identification
andPreservation
at NITE

Benefit Sharing
1. Monetary Benefit
2. Non-monetary Benefit

Workshop in Indonesia

Workshop in Vietnam

Using Molecular Information for the identification of microorganisms
by Dr. Hajime Sato (13-15 November 2007)

Workshop in Mongolia

Workshop in Myanmar

2005

Exchange of Researchers

NITE’s Non-Monetary Benefit
a. Sharing of research results (sharing ofinformation, co-author of Paper, etc.);
b. Delivery of equipments and suppliesc. Collaboration in sampling, isolation andcharacterization (human resourcedevelopment);d. Transfer of knowledge and technique byholding of on-site workshops;
e. Transfer of knowledge and technique toresearchers invited to NITE, Japan; etc.

Joint Research Project with Asian countries
MOU

Mongolia

Mongolia

P A2006～NITE
MOU

Myanmar

China
2005～

Vietnam
MOU

P A

MyanmarVietnam

2004～2006,
2013～

MOU
P A

2004～

Thailand2012～

Countries continuing the Joint
Research Program for theConservation and Sustainable Use of
Microbial Resources under MOU andPA

MOU

Indonesia
Indonesia
2003～

MOU
P A

Countries (BRCs) conducting
cooperative research under MOU orPA

１６

Thank you