RECYCLING PREPARATIVE HPLC

LC-92XX NEXT SERIESUSER'S MANUAL

Future of Polymer Science is it.

Japan Analytical Industry Co., Ltd.
URL http: //www.jai.co.jp/

E-mail: sales-1@jai.co.jp□M ai n O f f ic e： 〒190-1213

208 Musashi, Mizuho, Nishitama, Tokyo

TEL: 042-557-2331

FAX: 042-557-1892□Osaka Branch： 〒532-0002

5-13-8-303 Higashimikuni, Yodogawaku, Osaka city TEL: 06-6393-8511

FAX: 06-6393-8525□Nagoya Branch： 〒465-0025

3-609-3D Kamiyashiro, Meitoku, Nagoya city

FAX: 052-709-5403

TEL: 052-709-5400

RECYCLING PREPARATIV E HPLC

LC-92XX NEXT SERIESUSER'S MANUALPreface
Thank you for introducing Recycling Liquid Chromatograph model LC-92XX NEXT.
This manual contains instruction of the LC-92XX NEXT's operational manual,technical information and safety information.
Refer the manual thoroughly, for applying LC-92XX NEXT series to your researchand investigation with safety operation.
Keep the manual near by the LC-92XX NEXT Liquid Chromatograph for referring
any time.

Printing information
・No part of this document may be photocopied or reproduced, or translated toanother program language without the prior written consent of Japan AnalyticalIndustry Co., Ltd.
・Please note that the information contain in this document may be revised without
notice.
・Please contact our representative office or our company to make inquiries about
the information contained in this document.
・Manuals with missing pages or incorrect collating will be replaced.

Warranty Statement
JAI warrants the Recycling Preparative HPLC LC-92XX NEXT series as follows,
only when you operate it correctly following the instructions in this manual andwithout remodeling it.

Warranty period
LC-92XX NEXT Products are warranted for a period of one year from thedate of installation.

Warranty detailIf the product is found to be defective due to a manufacturing fault during
the warranty period, JAI will repair or replace the product or defective
parts without charge.

Negative assurance
Even during the warranty period, the defects caused by the following are
not covered by this warranty:
・Improper use.
・Operations under the condition not authorized in this document or ourcompany.
・Abnormal power supply or use under the conditions of irregular voltage
or frequency.
・Defect is coming from other factors, not from the LC-92XX NEXT.
・Use under severe environmental conditions such as corrosive gas, hightemperature and humidity, or continuous vibration.
・Flowing a highly corrosive liquid.
・Force majeure such as earthquake, fire, or lightning.
・Removal or transfer of the product after installation.
・Remodeling or Disassembly of the LC-92XX NEXT with not written way
in this manual.
・Using an instrument that is resold without our approval.
・Defects of the consumables or the parts of which the warranty period islimited are not warranted.
Parts of consumption ：Pump seal, pump plunger, pump plunger guide, pump check valve, line filter, manual
sample injector (rotor seal/stator face), selector valve (solvent selector, recycle flow 1,
recycle flow 2, drain, C/D selector)

The items which are not included in this paragraph are out of thewarranty.
Direct or indirect damages coming from the evident or implied warranties
of this instrument are not compensated.
Direct or indirect damages coming from destroy of the instrument are notcompensated.

How to Use This Document
This document "LC-92XX NEXT SERIES USER'S MANUAL" consists of the following
four volumes.
Choose the volume to use as needed.
Each volume has its own table of contents at the beginning.

Tab• Content

Daily
Operation
ManualGeneralInformation
Manual•Dailyoperations•Information for newusers•Functions
or otherbasic
information

Installation
ManualMaintenance
Manual•Installation•Maintenance•Relocation•Troubleshooting

To Operate in Safety
LC-92XX NEXT series instrument is a recycling preparative liquid chromatographto apply the separation analysis and separation collection of the samples by using
the principle of liquid chromatography.
Please read and protect to precaution for safely operations of the LC-92XX NEXT
series.

Cautions
・Do not use the LC-92XX NEXT series instrument other than a liquid
chromatography.
・Please read and adhere the instruction in this manual.
・Take safety measures to prevent an accident.
・Please note that the LC-92XX NEXT products that are remodeled ordisassembled without our prior notice are not warranted.
・In long term stop of the LC-92XX NEXT, disconnect Power cable. Seal column
ends with correct plugs to prevent column drying. Fill tubing with noncorrosive
solvent to prevent collision of tubing and flow line assemblies, but not flow thesolvent into the column.
・To avoid possible eye injury and skin trouble, wear eye protector and gloves.
・Prevent solvent leakage in the instrument.
・Do adequate ventilation in LC-92XX setting laboratory.

Support
Support on our Web site
Latest applications and soft ware revisions put on the site. News of our exhibition
and Events also put on the site.
We are awaiting your question, conference and request information of our
instrument.
Our Web site
http://www.jai.co.jp/

Emergency Measure
In emergency situation of the instrument or in disaster, do following emergency
measure.
Restart should be carefully to check the instrument performance. If you have any
worry, contact us or our representative.

Emergency stop・Turn POWER SWITCH off to stop the LC-92XX NEXT.
・Turn off every POWER SWITCH of peripheral instruments.
・Avoid solvent spill and leakage.
・Cut off the power supply.

VOLUME

1

Volume 1

Daily Operation Manual

Daily operations and information for new users

Contents
Volume 1 Daily Operation Manual
Chapter 1 Operation Flow ................................................ 1-1
Operation Panel Display ........................................................ 1-2
Operation Flow ....................................................................... 1-3

Chapter 2 General Operation ........................................... 1-4
Prepare the solvent, waste reservoir and sample. ................. 1-5
Turn the power switch on. ...................................................... 1-6
Press the [CLEAN UP] key..................................................... 1-6
Set parameters....................................................................... 1-7
Pump flow rate setting ................................................................. 1-7
Pump pressure limit setting .......................................................... 1-7
Detector setting ........................................................................... 1-8

Turn on the pump. .................................................................. 1-9
Turn on the integrator or recorder. ......................................... 1-9
Check the column stability. .................................................... 1-9
Inject the sample. ................................................................. 1-10
Separate recycles. ............................................................... 1-11
Collect the fractions.............................................................. 1-12
Shut down the LC................................................................. 1-13

Preface
This manual includes basic operation of the liquid chromatograph model LC-92XXNEXT series to control.
For more details of information, installation, and maintenance of the instrument, pleaserefer to other volumes, "General Information Manual", "Installation Manual", and"Maintenance Manual" on the tabs.

Cautions for Operation
・If a failure or wrong operation occurs in the LC-92XX NEXT, an error message
appears on the LCD display. At the beginning of the error message, "ERROR" isdisplayed followed by a number. Based on this number, refer to a section in"Volume 4 Maintenance Manual" to resolve the cause of failure.
・If you press the [Co/Dr] key for collecting fractions from a fraction nozzle during
the [RECYCLE] operation, an error message appears and fractions cannot becollected. Stop the [RECYCLE] operation, and then press the [Co/Dr] key in[FRACTION] to collect fractions.
Similarly, if you want to execute the [RECYCLE] operation during the[FRACTION] operation, stop [FRACTION] before executing [RECYCLE] bypressing the [Co/Dr] key in [FRACTION].
・For the keys located on the sheet switches other than [PURGE] and [AUTO
ZERO], you can press a key to execute its function, and press the key again tocancel the function.

1-1

Chapter 1

Operation Flow

Operation flow of LC-92XX NEXT series

1-1Operation Panel Display
This section describes the overview of the operation panel of the LC-92XX NEXT
series.

LCD Display

Arrow Keys

Displays numerical values such as
the pump flow rate, pressure, and UV or RI
signal.

Keys for changing the setting
values of the unit.

[OK] Key[CLEAN UP] key

Logo and Product Name

1-2

Operation Flow
Actual procedure is as follows.

Prepare the solvent, waste reservoir and sample.
Turn the power switch on.
Press the [CLEAN UP] key.
Set parameters.
Turn on the pump.
Turn on the integrator or recorder.
Check the column stability.
Inject the sample.
Recycle separation.
Collect the fractions.
Shut down the LC.

1-3

1-2

Chapter 2

General Operation

General operation flow of LC-92XX NEXT series

1-4Prepare the solvent, waste reservoir and sample.

Solvent
・Prepare sufficient volume solvent in a container.
・Place the solvent container in the LC-92XX solvent stage.
・Prepare the solvent purer than chemical grade reagent. If Chloroform (CHCL3) for
the solvent is recommended, CHCL3 added ethanol for stabilizer is suitable.(PureCHCL3 or CHCL3 added Amylene stabilizer is not recommended.)・In case of using mix solvent, mix each solvent completely.
・In case of using mix solvent, solvent mixture should be degassed perfectly. For
details of degassing, refer to "Appendix" P.2-48 in "General Information Manual".
・For the properties of typical solvent, refer to Appendix P.2-51 in "General Information
Manual".
・Filter the sample to remove micro particles with a suitable membrane. For details ofsolvent's filtration, refer to Appendix P.2-53 in "General Information Manual".
・Be careful to handle the solvent and waste solvent.
・Prevent solvent leakage in the instrument.
・Do adequate ventilation in LC-92XX setting laboratory.

Waste
・A waste reservoir volume should be more than the solvent container. Reservoir
should be chemical proof against the solvent.
・Do not the reservoir to turn over and spill solvent.

Samples・Sample should be filtered to remove particles before injection. For the recommended
sample filtration, refer to Appendix P.2-54 in "General Information Manual".

1-5Turn the power switch on.

・Make sure that the receptacle is 100 VAC and enough to supply power for the LC92XX. Turn power switch on.

Power Switch

・After 15 seconds from turning the power switch on, the computer in the LC-92XX
boots up to indicate the initial stage.

Press the [CLEAN UP] key.
・Press the [START] key of [CLEAN UP] at the lower right side of the operation panel.

・When the manual operation is required, a message appears on the LCD display withan intermittent beep.
・Follow the instructions displayed on the LCD display.
・After the completion of the [CLEAN UP] operation, turn the Column reject valve backto its original position ([CONNECT]).
*The [CLEAN UP] operation needs 10 to 15 minutes.
*While the pump is in operation, [CLEAN UP] cannot be executed. Execute the operation when the pumpis stopped.

1-6

Set parameters.

Pump flow rate setting
・Press the [FLOW SET] key in [PUMP].
・The parameter to be changed blinks on the LCD display.
・Use the arrow keys to set the flow rate value to use.
・Press the [OK] key.
*While the pump is in operation, the value cannot be changed.
Perform the operation while the pump is stopped.

Arrow Keys

Pump pressure limit setting
・Press the [MAXIMUM SET] key in [PUMP].
・The parameter to be changed blinks on the LCD
display.
・Use the arrow keys to set the maximum pressure(limit).
・Press the [OK] key.
・Similarly, press the [MINIMUM SET] key in the[PUMP] unit tab to enter the minimum pressure.
*The values can be changed while the pump is in operation. Set thevalues based on the Column pressure.
*The value "0" makes off pressure limit.
*Make sure the [MAXIMUM] value is more than the [MINIMUM]
value.

1-7

Detector setting
For UV DETECTOR・When using the recorder, press the [RANGE]
key and adjust the value by using theorarrow key.

For RI DETECTOR

・Normally use the RI detector with the [TEMP.C]
key turned ON (LED is turned on).
・After the [CLEAN UP] operation, the [AUTO
ZERO] operation is not yet executed. After thecolumn equilibrium, press both the [AUTO
ZERO] keys in the [UV DETECTOR] and [RI
DETECTOR] unit tabs.

1-8Turn on the pump.

・Press the [ON/OFF] key in [PUMP].

Turn on the integrator or recorder.
・Turn on the INTEGRATOR or RECORDER and monitor the detector signal. Make
sure to stabilize the base line of the detector signal.
・The INTEGRATOR or RECORDER is an optional product. Refer to each manual forcorrect operation.

Check the column stability.
・In case an RI detector is attached, make sure that the base line of the RI detector
signal is stable.
・JAIGEL-H series (20φ x 600 mm), W series(20φ x 500 mm), GS series (20φ x 500mm) the stability for each column, needs from 30 to 50 minutes. ODS and SIL
column should be waited with monitoring of the detector signal.
*In case of using the W and GS column, change of the solvent composition may need long time.
*Before the sample injection do zero adjustment referring to P.1-8.

1-9

Inject the sample.
・Prepare filtered sample. (For the typical filtering method, refer to "Micro
Particle Elimination in Sample Solution", Appendix P.2-54 in "Volume 2General Information Manual").
・Suck the sample solution in a syringe to pull a plunger.
・Wipe the drop out of the syringe needle with clean paper.
・Hold the syringe upwardly, push the plunger gently to purge air from thesyringe. Push the plunger until the air is purged thoroughly.
・Insert the syringe into the manual injector thoroughly until the syringe needle
touches the bottom.
・Turn the handle of the [MANUAL INJECTOR] to the [LOAD] position.
・Push the plunger thoroughly.
・Turn back the handle to the [INJECT] position and take the syringe.
・Clean the needle port with clean solvent. (For details on how to clean theneedle port, refer to "Needle Port Cleaning", Appendix P.2-54 in "Volume 2General Information Manual").

1-10Separate recycles.
・Monitor the chromatograph to wait a target peak
appearing.

・When the peak is rising, press

in [RECYCLE].

*The LED at the top of the key is turned on.

・At the end of the peak, press.

*The LED at the top of the key is turned off.

・When the peak is rising again, press[RECYCLE].

in・When you get an effective separation, press
stop the recycle operation.

to*Between the recycle operations, the handle of the manual sample injector should be located atthe [LOAD] position.
If the injector located at the [INJECT] position, the manual sample injector makes deadvolume to diffuse the peaks on the separation of the recycling.

1-11

Collect the fractions.
Siphon box (at lower left side front of the
instrument)
Siphon*Lamp turned off = Eluted from the siphon*Lamp turned on = Eluted from the fraction nozzle

Fraction nozzle
・Monitor the chromatograph to wait a target peak
appearing.
*Normally the drain flows from the siphon.

・Pressin [FRACTION].

・The LED at the top of the key blinks.
*The bar shows that a fraction of the sample is running dead volume
from the detector to the fraction nozzle.
*After running through the dead volume, the fraction flows from thefraction nozzle.

・Pressat the end of the peak.

*The fraction flows from the fraction nozzle until there is no fractionthat is running in the dead volume.

・Repeat the same operation for all the target peaks.

1-12

Shut down the LC.

・After collecting fractions, press the [ON/OFF] key in[PUMP] to stop the pump.

・Press the [START] key of [CLEAN UP] at the lower right side of the operation panel.

・When the manual operation is required, a message appears on the LCD display withan intermittent beep.
・Follow the instructions displayed on the LCD display.
・After the [CLEAN UP] operation, move the reject valve back to its original position tocomplete the [CLEAN UP] operation. If the LC-92XX NEXT will not be used for a longtime, stay the valve position at [REJECT].
*If the column stays a long time in the stopped flow line, solvent may be lost by gravity to dry the column
packing and to damage the column.

・Turn off the integrator, recorder or other attachment.
・Turn off the LC-92XX NEXT.

1-13

VOLUME

2

Volume 2

General Information Manual

General information of LC-92XX NEXT series (except optional accessories)

Contents
Volume 2 General Information Manual
Chapter 1 Description of LC-92XX NEXT ........................ 2-1
Composition of LC-92XX NEXT Series .................................. 2-2
Name and Function of Each Part ........................................... 2-3
Specification ........................................................................... 2-8
NEXT Series Features ........................................................... 2-9
Recycling preparative HPLC ........................................................2-9
Layout..........................................................................................2-9
Compact sized body.....................................................................2-9
Easy to operate............................................................................2-9
Auto-cleaning ............................................................................. 2-10
Non-diffusion function ................................................................ 2-10

Flow Channel Map ............................................................... 2-11
Operation Panel Display ...................................................... 2-14

Chapter 2 Description of Operation Panel ..................... 2-15
WAKE UP ............................................................................ 2-16
Description of Arrow Keys and [OK] Key.............................. 2-17
Description of Units .............................................................. 2-18
PUMP Unit ................................................................................. 2-18
Detector Unit .............................................................................. 2-21
UV Unit ...................................................................................... 2-21
RI Unit ....................................................................................... 2-24
FRACTION Unit ......................................................................... 2-26
RECYCLE Unit .......................................................................... 2-27

CLEAN UP ........................................................................... 2-28
LCD display during CLEAN UP operation ................................... 2-28

Chapter 3 Recycling Separation .................................... 2-31
Recycling Separation ........................................................... 2-32
Relation between Peak Separation and the number of
Recycling ................................................................................... 2-32
Note of good Recycling Separation ............................................ 2-34
For good Recycling efficiency..................................................... 2-37

Chapter 4 Condition Setting ........................................... 2-39

Setting Operation ................................................................. 2-40
Pump setting.............................................................................. 2-40
Setting UV detector .................................................................... 2-42
Setting RI detector ..................................................................... 2-44
Setting fraction ........................................................................... 2-46

Chapter 5 Appendix........................................................ 2-47
Degassing.................................................................................. 2-48
Property of typical organic solvent .............................................. 2-51
Micro particle elimination ............................................................ 2-53
Needle port cleaning .................................................................. 2-54

2-1

Chapter 1

Description of LC-92XX NEXT

LC-92XX NEXT compositions, operation flow-chart, and operation panel
functions

2-1

Composition of LC-92XX NEXT Series
The LC-92XX NEXT series is a high performance recycling preparative liquid
chromatograph for separation and purification.
The main body, pump, sample injector, column*, detector*, and integrator*
are installed as standard equipment.
High Resolution Hydrophobic SEC Column, High Resolution GFC
Column, High Resolution SEC Column, Optical Isomer separation
Column, Various Polymer Column, Partition and Adsorption Column
NEXT series can accommodate different separation method for bothanalytical and preparative scale.
NEXT series UV detector and RI detector can be fit into the body together.

*Columns, Detectors, integrator, and recorder are optional accessories. Please selectoptional accessories according to your purpose.
For details on using accessories, refer to separate instruction manuals for correct
operation.

2-2

Name and Function of Each Part

＜Front of the LC-92XX NEXT＞
Operation Panel

Manual Injector Valve

Column Reject Valve

Siphon Box

Pump Drain Valve

Name
Operation Panel

Function
To press the buttons to set conditions or execute theoperation.

Manual Injector Valve

To inject the sample manually.

Column Reject Valve

To disconnect the flow line from the column.

Pump Drain ValveTo drain the solvent from the pump outlet.

Siphon Box

To collect fractions from the fraction nozzle or thesiphon, or to collect the waste liquid.

2-3

<Inside of the LC-92XX NEXT Front Door>
Solvent Table
Pump HeadUV Detector
Power Switch
RI Detector
Wave Length
Tuner

Inside of
the Siphon
Box
Front Door

Name

Function

Pump Head
Solvent TableUV Detector*1
Power Switch
Wave Length Tuner*2
RI Detector*1

Siphon Box
Front Door

Device using suction and pressure to force solvent
into the flow line.
A table to place a solvent reservoir.
Detector for absorption of the light after passing
through the UV cell.
To turn on/off the power of the UV detector.
A tuner for wavelength range.
Detector for refractive index.
To collect fractions from the fraction nozzle or thesiphon, or to collect the waste liquid.
To protect inner units from damages and leaking
solvent.

*1 UV detector and RI detector are sold separately.
*2 Wave-length tuner is only available for variable wave length type UV detector.

2-4<Inside of the LC-92XX NEXT Right Side Column Cover>
Column Cover

Pre-Column Holder

Manual Injector Sample Loop

Column Holder

TO COLUMN Connector

FROM COLUMN Connector

Null Glass Adj. Hole

Name
Manual Injector
Sample Loop
TO COLUMN Connector
FROM COLUMN
Connector
Pre-Column Holder
Column Holder
Cut Filter Exchanger
Null Glass Adj. HoleCut Filter Exchanger

Function
A place to store sample temporarily right aftersyringe injection.
Connector of solvent's flow line from the bodyto columns.
Connector of solvent's flow line from thecolumns to the body.
To hold the Pre-Column.
To hold the Column.
A slit for switching UV wave length range, incase of installed UV-600 NEXT*1.
A hole for turning optical null glass to adjust the
optical zero point with screwdriver, in case of
installed RI-700 NEXT*1.

*1 UV detector and RI detector are sold separately.

2-5

<LC-92XX NEXT Left Side>
Output Port

Power Switch
Solvent Out

NamePower Switch
Output Port
Solvent Out*1

Function
To turn on/off the LC-92XX.
To output recognized signals from detectors
and valves.
Solvent out being connected to the fraction
collector. (Option)*1 The fraction collector is an optional product. Normally it is not used.

2-6

*1

<Inside of the LC-92XX NEXT Siphon Box Door>
SiphonPump Drain Valve
Fraction Nozzle

Siphon Box Door

Manual Sample Injector Drain Out

Pump Drain Out

Name

Function

SiphonOutlet of the eluent flowing from the detector.

Fraction Nozzle
Pump Drain Out
Manual Sample Injector
Drain Out

Nozzle for collecting eluent fractions from thedetector.
Drain out pipe for solvent from pump drain
valve.
To drain surplus solvent out when to injectsample into manual injector or to clean needle
port.

2-7

Specification
If the explanation or specification is the same for both LC-9210 NEXT and LC-9225 NEXT, it is written
as 92XX NEXT in this manual.

LC-9210 NEXT
Pump Model
Pump TypeMaximum Discharge
Pressure
Flow Rate
Operation Panel

FX-10

FX-25

Reciprocating double plunger
25 MPa20 MPa*0.1 to 10.0 mL/min

0.1 to 25.0 mL/min

8.4-inch LCD operation panel

Manual Sample Injector
Sample Loop (Standard)

LC-9225 NEXT

Rheodyne 7725i
3 mL

10 mL

Event Marker

Injection, recycle and collect

Recycle System

Built-inNon-diffusion Function

Twin valve method

Auto-Cleaning

Route washing by program

Dimension of Mainframe

464(W) x 492(H) x 504(D)WeightAprox. 38 Kg*In the LC-9225 NEXT, the maximum discharge pressure differs depending on the flow rate to be
used.
Flow Rate (mL/min)Maximum Discharge Pressure (MPa)0.1 to 9.9

2010.0 to 19.9

15

20.0 to 25.0

10

2-8

NEXT Series Features
This section describes the functional characteristics of LC-92XX NEXT
series.

Recycling preparative HPLCThis is a liquid chromatography for separation and purification.
"Recycling function" enables the same solvent and the sample toflow through columns more than one time to complete perfect
separation as if using "simulated" infinite columns.

Layout
The manual sample injector is located outside of the body, which
reduces time for tasks such as seal exchange and improves themaintenance. The pump and detectors can be taken out from thefront of the body more easily.

Compact sized body
Body size made 1/3 smaller compared to our previous models withimproved functions and performance.

Easy to operate
A 8.4-inch LCD operation panel is installed. The keys on theoperation panel are designed for easy intuitive operation.

2-9

Auto-cleaning
Automated flow line cleaning function keeps flow channels cleanand solvent changeover very easy.

Non-diffusion function
During extended recycling period, the recycled sample in the loop
can diffuse back into the solvent line with the danger ofcontaminating the solvent reservoir. The NEXT series incorporates
a patent pending non-diffusion device that prevents the sample
from backing into the solvent line. Thus, this keeps the lines ofNEXT series constantly clean automatically.

2-10

Flow Channel Map
The flow channel map of the LC-92XX NEXT series is as follows.

Valves and flow lines of LC-92XX NEXT

Manual Injector Valve

Solvent

Column Reject Valve
Buffer Loading Valve

Pump

Buffer Volume
Pump Drain Valve
Recycle Valve 2

Recycle Valve 1

Collect Valve
Solvent Filter

Detector

Buffer Solvent Drain
ColumnBuffer Solvent Drain

SiphonFraction Nozzle

2-11

<Normal Flow Channel via Siphon><Normal Flow Channel via Fraction Nozzle>

2-12

<Recycle Flow Channel>

2-13Operation Panel Display
This section provides the overview of the operation panel of the LC-92XXNEXT series.

LCD Display

Arrow Keys

The setting values and monitored values

Keys for changing the setting

are displayed.

values of units.

[OK] Key

Unit Tab
The unit tabs and theiroperation keys

[CLEAN UP] Key

Logo and Product
Name

2-14

2-2

Chapter 2

Description of Operation Panel

Description of the LC-92XX NEXT series operation panel

2-15

WAKE UP
WAKE UP is the state when the power is turned on.

It refers to the initial screen of the LCD display that starts when the powerswitch is turned on.
The initial screen changes to the normal screen after approximately 15
seconds from power on.
<Example: WAKE UP of LC-9210 NEXT>
Power onInitial screen 1

HE L LO !
LC-NEXT SER I ES

H E L L O !
L C - N E X T( C ) JA I

( C )Initial screen 2

HE L LO !
LC- NEXHE L LO !
LC-NEXT SER I ES
LC- 9 2 1 0( C ) JA I(CHE L LO
LC- NE

Normal screen

F L OWPRESS
UV
RI

Flow rate

0 9 . 9 0 m l ／m i n
0 . 0 MP a
0000
0000

(
Monitoring pressure
UV output
RI output*The LC-9210 NEXT model is used as example.
*While the initial screens 1 and 2 are displayed, the system performs the initialization,verification of the previous settings, and preparation.
*Key operations are not available on the initial screen 1 or 2.

2-16

Description of Arrow Keys and [OK] Key<How to Operate Arrow Keys>: Increment a numerical value: Decrement a numerical value: Shift the blinking cursor to the right: Shift the blinking cursor to the left

The arrow keys are mainly used to change various setting values.
The up arrow key increments the numerical value at the blinking
cursor, and the down arrow key decrements it. The right arrow key
shifts the blinking cursor to the right, and the left arrow key shifts itto the left. After setting, press the [OK] key to update the setting
value.

<How to Operate [OK] Key>This key is used to update the setting values. After setting a
numerical value, be sure to press the [OK] key.
*When the manual [CLEAN UP] operation is required or when an error message is displayed,
the LED blinks at the top of the key.

2-17

Description of Units

PUMP Unit
・In the [PUMP] unit, there are thekeys for starting/stopping thepump, and displaying thesetting screens of the flow rate,
the maximum pressure limit,
and the minimum pressure limit.

<Starting/Stopping the Pump>

This is the key for starting/stopping the pump. Press
to start
the pump, and press
again to stop it. At the top of the key,
the LED is turned off when the pump is OFF (stopped) and turnedon when the pump is ON (running).

2-18

<Setting Pump Flow Rate>F L OW R A T E S E T
0 9 . 9 0 m l ／m i n

F L OW R A T E S E T - U P
0 9 . 9 0 m l ／m i n( M A X : 1 0 . 0 0 m l ／m i n )( M A X : 1 0 . 0 0 m l ／m i n )F L OW
PRESS
UV
RI

0 9 . 9 0 m l ／m i n
0 . 0 MP a
0000
0000

F L OW R A T E S E T - U P
0 9 . 9 0 m l ／m i n

F L OW R A T E
09( M A X : 1 0 . 0 0 m l ／m i n )( MA X : 1 0 . 0

Press the [FLOW SET] key to display the setting screen in the LCD
display. Use the arrow keys to set the numerical value at theblinking cursor. After setting, press the [OK] key to update thesetting value.

2-19

<Setting Pump Pressure Limits>

M A X I M UM S E T

M I N I M UM S E T
0 2 . 0 MP a0 0 . 4 MP a

( M I NS E T V A L ： 0 0 . 0 MP a )( MA X S E T V A L ： 0 2 . 0 MP a )The current pressure is displayed in the normal screen of the LCD display.
Press the [MAXIMUM SET] key to set the maximum pressure limit.
Press the [MINIIMUM SET] key to set the minimum pressure limit.
M I M I M A M

S E T<Setting Maximum Pressure Limit>
0 0 . 1M
( M A andX : detectors.
5 0 . 0 0
M P a )
When it's over pressed, this function is to protect columns
Press the [MAXIMUM SET] key to display the setting screen in the LCD
display. Use the arrow keys to set the numerical value at the blinking
cursor. After setting, press the [OK] key to update the setting value. Whenthe maximum pressure limiter is activated, the whole system stops and anerror message appears.
*"(MINSETVAL: 00.0 MPa)" displayed in the setting screen indicates the setting value of theminimum pressure limit<Setting Minimum Pressure Limit>
When it's pressed too low, this is to protect columns and solvent fromsplitting. Press the [MINIMUM SET] key to display the setting screen in
the LCD display. Use the arrow keys to set the numerical value at theblinking cursor. After setting, press the [OK] key to update the setting
value. When the minimum pressure limiter is activated, the whole systemstops and an error message appears.
*"(MAXSETVAL: 00.0 MPa)" displayed in the setting screen indicates the setting value of the
maximum pressure limit.
*For details on how to operate the arrow keys, refer to P.2-17.
*In case that the pressure limiter is activated to stop the system, refer to "Volume 4
Maintenance Manual" for trouble shooting.
*The maximum value cannot be less than the minimum value, and they cannot exceed
50.1 MPa.

2-20

M I M I M

( M

Detector Unit
RI DETECTORUV DETECTOR

・In the [UV DETECTOR] and [RI DETECTOR] units, there are the keys foradjusting the current UV/RI output to O mV and setting the recordersignal range.

<Output Value Display Screen>F L OW
PRESS
UV
RI

0 9 . 9 0 m l ／m i n
1 . 0 MP a
0210
0035

F L OW
PRESS
UV
RI

0 9 . 9 0 m l ／m i n
0 . 0 MP a
UV output
0000
0000
RI output

・In the LCD display, the current UV/RI output is displayed in mV unit.

UV Unit・In the UV unit, there are the [AUTO ZERO] key for adjusting the UV
output to 0 mV, the [RANGE] key for setting the recorder signal range,
and the [SET] key for setting the detector response speed.

2-21<Zero Adjustment Key>・Press the [AUTO ZERO] key to adjust the current UV output to 0mV.
However, when the UV transmittance is too low, the AUTO ZERO
function may not work.

<Recorder Signal Range Adjustment>

UV

RANGE

↑ : RANGE↓ : RANGE

SET
x001
UP
D OW N・This function is used for output signal attenuation of pen recorder. Pressthe [RANGE] key to display the setting screen in the LCD display.
Press thearrows key to increment the signal range (the peak will
be shown smaller), or
to decrement it (the peak will be shownlarger).

*In case of monitoring the chromatogram with integrator, recorder signal range
buttons are without the need for setup.
*In case a peak-sense (sense threshold and peak height) fraction collector(optional product) is used, the range need to be adjusted.

2-22

<Brightness Check>

UV S E T*ME NUP A R AM E T E R
BACKUV S E T ( M E N U )* S AMP L E
R E F E R E NC E
BACK

1000
1500

・This function is used to perform the brightness check of the lamp
installed on the UV detector. Press the [SET] key and select [MENU] todisplay the output values of [SAMPLE] and [REFERENCE].
*If the [REFERENCE] value is too low, the lamp should be replaced.

<Setting Detector Response Speed>

D O

Y O U

S E T
Y E S

UV S E T
ME NU* P A R AM E T E R
BACKU V S E T ( P A R AM E T E R )* R E S PONS E
MID( S L OW←→M I D←→F A S T )
BACK

U V

S E T

・This function is used to set the detector response speed. Press the* M E N U
[SET] key and select [PARAMETER] to display the response setting
P A R A M E T
screen. You can select the response speed from the three levels,[SLOW], [MEDIUM], and [FAST]. They mean the time constants of 1.5

E R

sec, 0.5 sec, and 0.05 sec respectively. To change the setting, press
theorarrow key.

2-23

U V
S E T
M E N U
* P A R A M E T E R

RI Unit

・In the RI unit, there are the [AUTO ZERO] key for adjusting the RI
output to 0 mV, the [RANGE] key for setting the recorder signal range,
the [PURGE] key for replacing the solvent in the reference cell, and thekeys for selecting the polarity and whether or not to control thetemperature.

<Zero Adjustment Key>・Press the [AUTO ZERO] key to adjust the current RI output to 0mV.
When the optical balance is lost too much, the AUTO ZERO function
may not work.
*If the zero adjustment function does not work, refer to "Volume 4 Maintenance
Manual".

<Recorder Signal Range Adjustment>

・Adjust the recorder signal range in the same way as described in"<Recorder Signal Range Adjustment>" of the UV unit. For details, referto the page 2-22.

2-24

<Starting PURGE>

・This key starts/stops replacing the solvent in the reference cell. Press
to change the flow channel to the reference cell for one minute. At
the top of the key, the LED is turned off when the fraction is flowing
through the sample cell, and turned on when it is flowing through thereference cell.
*During the [CLEAN UP] operation, the solvent in the reference cell is also
replaced automatically. This PURGE function may not be used in general
operation.

<Starting TEMP.C>

・This function is used to select whether or not to use the temperatureadjustment function of the RI detector. When the LED is turned on, thetemperature controlling heater is turned on to maintain the insidetemperature as 35℃ for stable operation. Normally this function should
be turned on. When you use a low boiling solvent, you can press the[TEMP C] key to turn it off.

<Selecting SIGNAL POLARITY>

・There are cases that current RI output is shown in negative, when thesolvent with high index of refraction is used for mobile phase. This key
can be used to reverse the negative signal to positive. When the LED isturned on, the signal is output as it is. Press the [POLARITY] key to turnthe LED off and reverse the polarity from negative to positive.

2-25

FRACTION Unit

・In the [FRACTION] unit, there are
the keys for selecting the methodof collecting fractions (siphon or
fraction nozzle) and setting thedead volume.

<Setting DEAD VOLUME>

D E A D V O L UM E
* UV

SET
0 1 . 9m l

( UV ←→RI ←→ ELSE )・You can use this key to select the detector that detects the peak forthe basis of collecting fractions. Press theorarrow key
to select a detector. The time difference is calculated as deadvolume from the selected detector to a fraction nozzle. If you select[ELSE], you can set an arbitrary value when pipe lines are extended
or other detectors are installed. To set an arbitrary value, press
and select [ELSE] to display the setting screen. Use the arrow keys
to set the numerical value at the blinking cursor.
*For details on how to operate the arrow keys, refer to P.2-17.
*The default value is preset in case our optional products (UV-254 NEXT,
UV-370 NEXT, or UV-600 NEXT for UV detector, and RI-700 NEXT for RI
detector) are installed.
*[ELSE] can be used when other detectors or non-standard-equipped fraction
collectors are installed.

2-26

<Siphon/Fraction Nozzle Selection Key>・You can use this key to collect the fractions of eluent from the detector
by using the siphon or the fraction nozzle. The LED is turned off for theeluent from the siphon, and turned on for the eluent from the fraction
nozzle. To collect fractions with the fraction nozzle, press
when
the LED is off. Then the LED blinks, which indicates that the fraction is
passing through the dead volume specified by [DEAD VOLUME SET].
After passing through the dead volume, the eluent will flow from thefraction nozzle. If you press
again, the LED blinks in the sameway, and the eluent passes through the dead volume and then flows
from the siphon.

RECYCLE Unit
・This is the control unit of therecycle valve. You can use the key
to start/stop the [RECYCLE]
operation.

<Starting RECYCLE>
・Pressto start the [RECYCLE] operation, and pressagain

to stop the operation.
At the top of the key, the LED is turned off
when the [RECYCLE] operation is stopped (normal flow channel), andturned on when the [RECYCLE] operation is running.

2-27

CLEAN UP
Press the [CLEAN UP] key at the lower right of the operation panel toexecute the flow line cleaning automatically.

LCD display during CLEAN UP operation

S AMP L E

L OO P C L E A N I NG

CLEAN UP operation status

50%
E ND

S TART

CLEAN UP progress

In the LCD display, there are the two sections; one indicates the place
where the [CLEAN UP] operation is being executed, and the other theoperation progress.

<STATUS Display>
The upper section of the LCD display shows the place where the[CLEAN UP] operation is being executed. For information on what thedisplay indication means, refer to the following table:
Display indication

Where to clean

1.PUMP PURGE

Solvent filter to Pump head

2. BUFFER CLEANING

Buffer volume

3.SAMPLE LOOP CLEANING

Manual injector sample loop

4.VALVE CLEANING

Recycling valve

5.RI PURGE

RI reference cell

6.FINISH CLEANUP PROGRAM

2-28

<CLEAN UP Progress>
S AMP L E

L OO P C L E A N I NG
50%

S TART

E ND

The lower section of the LCD display shows the progress of the[CLEAN UP] operation. 100% means the completion of the operation.
<Emergency Stop>
If you press the [CLEAN UP] key during the [CLEAN UP] operation,
the LED is turned off, and the operation is stopped forcibly. Pleasepress the key only in emergency situation. After the emergency stop,
the display returns to the normal display.
<How to Operate>
When you press the [CLEAN UP] key, the operation procedures are
displayed in the LCD display. Please follow the instruction. When amanual operation is required or when you need to verify settings, amessage appears in the LCD display with an intermittent beep. The[CLEAN UP] operation ends in 10 to 15 minutes. After the completion,
turn the column selector valve back to its original position([COLUMN]) to use it.

2-29

<How to Use Solvent Suction Attachment>
In the LC-92XX NEXT, you must inhale the solvent by using a solvent
suction attachment (standard accessory) at the preparation for the[CLEAN UP] operation.

Solvent Suction Attachment<How to Use>
CLEAN UP preparation screen

1. When you press the [CLEAN UP]
key, the operation procedures 1 - 5
are displayed in the LCD display.

5 P L E A S E P R I M I NG MUS T
B E DON E .
PUSH

"OK "

KEY ( 5 / 5 )When you check and implement theitems 1 to 4, then the item 5
instructs you to inhale the solvent.

Siphon Box

2. Set the solvent suction attachment
to the pump drain out inside of thesiphon box.
Pump Drain

3. Set the luer-lock syringe to theOutsolvent suction attachment.
4. Pull the syringe to inhale the solvent(at least 10 mL of solvent must be
inhaled).

Metal Part

5. After inhaling the solvent, remove
the solvent suction attachment (pullout it while pushing the metal part

Solvent Suction Attachment＋

located at the top of the pump drainout), and then press the [OK] key tostart the [CLEAN UP] operation.

Luer-lock Syringe

2-30

2-3

Chapter 3

Recycling Separation

Description of recycling separation, setting of recycling condition
parameter

2-31

Recycling Separation
The recycling system is a standard specification of the LC-92XX NEXT
series.
The recycling system is the feature that has the column eluent flow intocolumn inlet again.
If the separation is insufficient in normal HPLC separation, the change isrequired in column (separation mode, length, particle size of packing)and/or solvent condition (solvent polarity, gradient curve, salt
concentration).
Recycling system achieves hi-separation without change of such column
condition or solvent condition as the HPLC case.
Insufficient separation area is introduced into the same column once
more, and sufficient separation achieves.

Relation between Peak Separation and the number of
Recycling
In stead of using multiple columns, equivalent separation executes with
the Recycling separation. As shown below, separation is multiplied
according the numbers of the recycling.

Recycling separation:
COLUMN: JAIGEL-1H -F+JAIGEL-2H-F
FROW RATE: 3.5 ml/min
SAMPLE: Polystyrene oligomer (Mw. 495, Mw/Mn 1.20)

2-32

Separation efficiency with n times Recycling is shown following formula.
2(n+1) (TR2-TR1)
Rn+1={(n+1) W 12+nW E2}1/2+ {(n+1) W 22+nW E2}1/2
Rn+1
W1
WE
W2

: Separation efficiency with n times Recycling: Width of Peak 1: Peak diffusion width of flow line without column *: Width of Peak 2

TR1
TR2

: Retention time of Peak 1: Retention time of Peak 2

T1

T2

Chromatograph

Peak shapes of Peak 1 and Peak 2

W2
W1* When a column more than 20φ i.d. is used, W E does not affect the separation because it is negligible
smaller than the column volume. When Recycling executes many times, turn the MANUAL INJECTOR
knob to [LOAD] position for suppression of peak diffusion.

To take a separation such as the figure, Rn+11.5 is need.
2

2 1/2

*Peak width "W" with n times of recycling spreads to W(n+1)= {(n+1) W +nW E } .

2-33

Note of good Recycling Separation
Recycling separation may have the following troubles. Before the real
Recycling, pretest is recommended for the chromatogram acquisition
without Recycling.

When the Recycling area is too wide or the Recycle times are too much,re-mixture trouble may occur. The top of recycled area will mix with thetail of the recycled area. Recycling area and times should be suitablevalue.

1st Cycle

2nd Cycle

3rd Cycle

5th Cycle
4th Cycle
6th Cycle

Re-mixture

Re-mixture

2-34

<Overlap Trouble>
If the Recycling area is set too fast retention time, 2nd cycle peak get
past the 1st peak (symptom 1) or 2nd cycle peak overlaps with the later
peak (symptom 2). Pretest is recommended for the chromatogram
acquisition without Recycling to avoid overlap troubles.

Symptom 1

Symptom 2

1st Cycle peaks

A

without A

Recyclechromatograph

Only A peak group
Recycle chromatograph

Normal chromatograph2-35

<Contamination Trouble>
The LC-92XX NEXT series standard specification has the backflow
prevention system. The backflow prevention system works to reduce
contamination during the recycle operation for good recycle separation.
The backflow prevention system has a buffer-volume. The buffer-volume
is cleaned during CLEAN UP operation. When the CLEAN UP isneglected or high-viscosity sample/low soluble sample is recycled,
shock peak may appear at change over of the Recycling valve. Whenthe shock peak appears, do CLEAN UP operation again. If the symptom
cannot be remedied, cleaning-maintenance is required. Please refer toVolume 4 "Maintenance Manual".

Recycle Chromatogram with contaminations.

0

20

40

60

Recycle Chromatogram after CLEAN-UP

0

20

40

Rt (min)

2-36

80

100

120 Rt (min)For good Recycling efficiency
During Recycling separation, recycled sample flows unique flow line thatthe normal HPLC is not used, rear flow of the Detector, the Pump, andthe Injector flow line.
Select suitable instrument and turn over the knob of the MANUALINJECTOR to LOAD.
<Select Suitable Instrument>
In the LC-92XX NEXT series, the LC-9210 NEXT and LC-9225 NEXTare selected according to the Column size.
Double plunger reciprocal style pump is applied to the LC-92XX NEXT
series. For the reduction of the diffusion, select suitable LC according
Column size.
Sample diffusion in the plunger room can be ignored when the Column
inside volume is enough larger than the plunger volume.
To Column
Plunger
It moves frontward and backward and solvent ispressurized.
The volume differs depending on the specification.

Plunger Volume
Structure of the Plunger

Table of Suitable Column Size φ for Recycling Separation
LC
Separation mode
SEC (Size exclusion
chromatograph)
Partition/Adsorption/Other mode

LC-9210 NEXT

LC-9225 NEXT

10φ  column id  30φ

10φ column id  50φ

10φ column id  20φ

10φ column id  30φ
LC

Type

LC-9210 NEXT

LC-9225 NEXT

Plunger volume

32 μL x 2

77μL x 2

2-37

<Turn Over the Knob of the MANUAL INJECTOR to LOAD>
The LC-9210 NEXT has a 3 mL-Sample loop, and the LC-9225 NEXT
has a 10 mL-Sample loop as a standard.
When multiple times Recycling is done, turn over the knob to LOAD toeliminate the sample loop from the flow line because the recycled
sample diffuse in the sample loop.
Wait the manual injector knob at the [INJECT] position while injecting thesample, cleaning the needle port, and filling the sample loop with the
new solvent. After that, turn the manual injector knob to the [LOAD]
position.
When the pump flow rate of the LC-9210 NEXT is set at 3.0 mL/min ormore (10 mL/min or more for the pump flow rate of the LC-9130 NEXT),
wait for one minute after the injection, and then turn the manual injector
knob to the [LOAD] position to eliminate the sample loop from the flowline.
Sample Loop

Sample LoopIn the Flow LineOut of the FlowLine

From Pump

From Pump

To Column

To Column
INJECT

LOAD

2-38

2-4

Chapter 4

Condition Setting

How to set conditions of LC-92XX NEXT series

2-39

Setting Operation
Pump setting

Set the pump flow rate and the pump pressure limit in the [PUMP] unit.

<Setting Pump Flow Rate>
・Press the [FLOW SET] key.
・The parameter to be changed blinks on the LCD display.
・Set the flow rate for the column.

<Setting Pump Pressure Limits>
・Press the [MAXIMUM SET] key].
・The parameter to be changed blinks on the LCD display.
・Set the value at which the pressure maximum limiter works.
The pressure maximum limiter stops the pump when the pump pressure rises over thelimit, so as to prevent a column and detector cell damage from the over pressure comingfrom injection of dust and precipitated insoluble in a column. An error message appears
when the limiter works.

・Press the [MINIMUM SET] key.
・The parameter to be changed blinks on the LCD display.
・Set the value at which the pressure minimum limiter works.
The pressure minimum limiter stops the pump when the pump pressure drops below thelimit, so as to prevent a column damage or solvent spill when the solvent loses or leakageof system occurs. An error message appears when the limiter works.

2-40

<Limit of Setting Value>
The setting values of flow rate and pressure limits is limited in accordancewith the LC model and the set flow rate.
Refer to the following table. When the setting value is over the limitation, anerror message appears.

Flow Rate

LC-9210 NEXT

Flow Rate

LC-9225 NEXT 10.0 mL/min 25.0 MPa

 9.9 mL/min 20.0 MPa

 19.9 mL/min 15.0 MPa

 25.0 mL/min 10.0 MPa

The flow rate can be set in the ranges of 0.1 to 10.0 mL/min and 0.1 to 30.0
mL/min for LC-9210 NEXT and LC-9225 NEXT, respectively.
To stop a pressure limiter, set [0 MPa].

CautionWhen the Pump is running, the flow rate cannot be changed. Stop the Pump and reset theflow rate. When the pump flow rate is changed, change the [MAXIMUM] pressure limit to"monitoring pressure + 2.0 MPa" or more, and the [MINIMUM] pressure limit to "monitoringpressure - 2.0 MPa" or less.

2-41

Setting UV detector
In the [UV] unit, the UV-370 NEXT, UV-254 NEXT, or UV-600 NEXT
function can be set.

<Recorder Signal Range Adjustment>
・This function is used for output signal attenuation of pen recorder. Pressthe [RANGE] key to display the setting screen in the LCD display. Pressthearrows key to increment the signal range (the peak will be shownsmaller), or
to decrement it (the peak will be shown larger).

*In case of monitoring the chromatogram with integrator, recorder signal range
buttons are without the need for setup.
*In case a peak-sense (sense threshold and peak height) fraction collector(optional product) is used, the range need to be adjusted.

2-42

<Wavelength Selection>
・Pull the front door forward.

UV Detector

Front Door

Pull forward

Wavelength
Selector
Locking Pin

Close
Release・Remove the locking pin of wave-length selector inside the front door.
・Turn the wave-length selector to set the wave-length.
・Tighten the locking pin.
・Close to the front door.
・Press the [AUTO ZERO] key in [UV DETECTOR].
*Setting method of UV-370 NEXT is above
mentioned. Model UV-254 NEXT detector uses only
one wave-length and not selected wave length.
*Select the wave-length at where using solvent has
low wavelength absorption. 0 adjustment may not doat the wave-length where the solvent has highabsorption. Please refer P.2-51, for the typicalsolvent UV cut-off wavelength.
*Setting cut filter (Only UV-600 NEXT)

Wave-length

Cut filter

199 nm ～ 399 nm

Push

400 nm ～ 600 nm

Pull

2-43

Setting RI detector
In the [RI] unit, the RI-700 NEXT function can be set.

<Recorder Signal Range Adjustment>
・This function is used for output signal attenuation of pen recorder. Pressthe [RANGE] key to display the setting screen in the LCD display.
Press thearrows key to increment the signal range (the peak will

be shown smaller), or
larger).

to decrement it (the peak will be shown*In case of monitoring the chromatogram with integrator, recorder signal range
buttons are without the need for setup.
*In case a peak-sense (sense threshold and peak height) fraction collector(optional product) is used, the range need to be adjusted.

<Setting SIGNAL POLARITY>
・When the LED at the top of [POLARITY] is turned on, the output has thesame polarity as the original signal. Press the [POLARITY] key to turnthe LED off and reverse the polarity of the output signal.
*Reverse polarity is profitable when high refractive index solvent like toluene is used foreluent.
*Please refer P.2-51, for the typical solvent refractive index.

2-44

<Setting TEMP C>
・When the LED is turned on, the temperature controlling heater of thedetector is on to maintain the temperature at 35℃. Press the [TEMP C]
key to turn the LED off, which indicates that the temperature is notcontrolled.

*When the LED is turned on, more stable RI signal is obtained. Normally, the LED should
be turned on.
*Using low boiling point solvent, turn the LED off to suppress bubbling of the solvent.
*Please refer P.2-51, for the typical solvent boiling point.

2-45

Setting fraction
In the [FRACTION] unit, the dead volume can be set.

<Setting DEAD VOLUME>
・Press the [DEAD VOL SET] key.
・Selecting [UV], set the preset dead volume between UV-370 NEXT,
UV-254 NEXT, or UV-600 NEXT, RI-700 NEXT, and Nozzle of Fraction
Collector.
・Selecting [RI], set the preset dead volume between RI-700 and Nozzle ofFraction Collector.
・When the external detector is used, or when the fraction collector isremoved, select [ELSE] to set the dead volume.
・The specified dead volume is used to automatically calculate the deadvolume flow time when the valve to be used is switched between thefraction nozzle and the siphon.
*The default value is preset in case our optional products (UV-254 NEXT, UV-370
NEXT, or UV-600 NEXT for UV detector, and RI-700 NEXT for RI detector) areinstalled.
*If [0.0] value is selected, the dead volume (delay time) is neglected. Immediately afterpressing the [Co/Dr] key, the valve to be used is switched between the fraction nozzle
and the siphon.
*When the [RECYCLE] operation is switched to ON/OFF, the dead volume (delay time) isneglected. Immediately after pressing the [RECYCLE] key, the valve is switched.

2-46

2-5

Chapter 5

Appendix

Degassing, property of typical organic solvent, elimination of micro
particles

2-47

Degassing
Solvent to use an eluent in the LC-92XX should be degassed. Pressure
fluctuation of the peak retention time or the detector noise may occur withthe babble in undegassed solvent. To result best chromatograph without
noise and fluctuation, the solvent to use an eluent should be degassed.

<Degassing with ON-LINE DEGASSER>
・The ON-LINE DEGASSER should be connected between the solvent
container and LC-92XX NEXT at the solvent supplying connector.
・Read the ON-LINE DEGASSER instruction and according to the
instruction, connect the ON-LINE DEGASSER to the LC-92XX NEXT.
・According to the instruction, turn the power switch on to make sure that theAC voltage is suited to the DEGASSER.
Be care that the DEGASSER is chemical proof against the solvent
according to the instruction.
Strong organic solvent is sometime highly corrosive to the DEGASSER.
In case the solvent which is corrosive to the DEGASSER, do degassing
with an Ultra sonic cleaner and or vacuum pump described below.
In water solvent operation, the degassing may not be sufficient by the
ON-LINE DEGASSER due to the flow rate and laboratory temperatureenvironment.
Degassing with an Ultra sonic cleaner and or vacuum pump is also
recommended.
The above method is a typical connecting method.
The connecting method may differ depending on the type of degasser orpipe line handling because these factors may cause a different filtering
resistance.
Make sure to apply the suitable connection without much flow resistanceaccording to the instruction.

2-48

<Degassing with Ultra Sonic Cleaner>・Move the solvent into the container. In case of
mixture solvent, stir the solvent thoroughly.
Without stirring the mixture solvent, thereproducibility of the chromatogram may be lost,
because the lost of the component may occurs.
・Solvent such as sealed containers without lids,
and a lid on things such as Kleenex for good
ventilation.
If the seal is inflated when the mixing
temperature ultrasonic, solvent containers may
be destroyed.
・Dip the container in the Ultra sonic cleaner. Ultra sonic cleaner should be
half full with water.
・Operate an ultra sonic cleaner in 10 to 15 min. When there is no bubbling,degassing is complete.
When bubbles stuck on the wall of solvent container, blow N2 or He2 gas
in the solvent easy to remove the bubbles. After blowing, operate an ultra
sonic cleaner in few minutes.
In case of the solvent mixture, keep in short time operation of the ultra
sonic cleaner less than 30 min.
Vaporization of the solvent to change composition of the mixture occurs inlong operation of the ultra sonic cleaner.
・Wipe the drops out of the container and place the container on the solvent
stage in the LC-92XX NEXT.
Dissolvent of the air into the solvent occurs soon after degassing. Do
degassing just before use the solvent.

2-49

<Degassing with Ultra Sonic Cleaner Plus Vacuum>
・Move the solvent into the container. In case of
To aspirator
mixture solvent, stir the solvent thoroughly.
Without stirring the mixture solvent, thereproducibility of the chromatogram may be lost,
because the lost of the component may occurs.
The solvent container must be vacuum tight. A
conical beaker or conical flask may bedamaged at decompression.
・Vapor from the solvent contains organic vapor. To prevent spill of theorganic vapor to swage, use closed system aspirator to make vacuum.
Waste water should be disposed properly according to laws andregulations in the same manner as organic solvents.
・Place the container in the ultra sonic cleaner, and seal it with a rubber
stopper that is fitted into the container mouth. Set a vacuum line to therubber stopper, and connect its one end to the aspirator.
・Perform the ultra sonic cleaning, and operate the aspirator to reduce thepressure of the container. Operate both the ultra sonic cleaner and a water
aspirator in 5 min.
Solvent component will be change while the vacuum-filtration. Keep thecondition of the filtration
time and vacuum condition. Keep the vacuum degree and time inconstant.
・Wipe the drops out of the container and place the container on the solvent
stage in the LC-92XX NEXT.
Dissolvent of the air into the solvent occurs soon after degassing. Do
degassing just before use the solvent.

2-50

Property of typical organic solvent<Solvent Property List>
Solventε(Al2O3)

Viscosity

UV limit

Refractive

Boiling(mPa s;to use

index

point

20℃)

(nm)

(20℃)

(℃)

Solventε(Al2O3)

Viscosity

UV limit

Refractive

Boiling(mPa s;to use

index

point

20℃)

(nm)

(20℃)

(℃)

Pentane

0.00

0.24

200

1.357

36.1

Acetone

0.56

0.30

330

1.359

56.3

Hexane

0.01

0.31

200

1.375

68.7

Methyl ethyl ketone

0.51

0.42

329

1.379

79.6

Heptane

0.01

0.42

200

1.388

98.4

iso-Butyl methyl ketone

-

0.54

334

1.396

116.5

2,2,4-triMethylpentane

0.01

0.50

215

1.391

99.2

Diethyl ether

0.38

0.24

218

1.352

34.6

Nonane

-

0.72

200

1.405

150.8

Diisopropyl ether

0.28

0.37

220

1.369

69.0

decane

0.04

0.92

210

1.412

174.1

Tetrahydrofuran

0.45

0.55

212

1.407

66.0

Cycropentane

0.05

0.47

210

1.409

49.3

1,4-Dioxane

0.56

1.44

215

1.442

101.3

Cycrohexeane

0.04

0.98

200

1.426

80.7

Propylene carbonate

-

-

-

1.419

240.0

Benzene

0.32

0.65

278

1.501

80.1

Methanol

0.95

0.55

205

1.328

64.7

Toluene

0.29

0.59

284

1.497

110.6

Ethanol

0.88

1.20

210

1.361

78.3

o-Xylene

0.26

0.81

288

1.505

144.4

1-Propanol

0.82

2.26

210

1.386

97.2

m- Xylene

0.26

-

290

1.496

138.5

2-Propanol

0.82

2.86

205

1.377

82.3

Dichloromethane

0.42

0.45

233

1.424

39.8

2-Methoxyethanol

-

1.72

210

1.402

124.6

Chloroform

0.40

0.58

245

1.446

61.2

1-Butanol

-

2.95

215

1.399

117.7

Carbon tetrachloride

0.18

0.97

263

1.460

76.8

2-Butanol

-

4.21

260

1.397

99.6

1,2-Dichloroethane

0.49

0.79

230

1.445

83.4

Isobutanol

-

4.70

200

1.396

107.7

Trichloroethylene

-

0.57

273

1.477

87.2

2- Ethoxyethanol

-

2.05

210

1.408

135.6

Tetrachloroethene

-

0.93

295

1.506

121.2

Acetonitrile

0.65

0.34

190

1.344

81.6

n-Butyl chloride

-

0.47

220

1.402

78.4

Diethylamine

0.63

0.38

275

1.387

56.0

Chlorobenzene

0.30

0.80

287

1.525

131.7

N,N-Dimethylformamide

-

0.92

268

1.430

153.0

-

1.32

295

1.551

180.5

N,N-Dimethylacetamide

-

2.14

268

1.438

166.1

0.15

0.37

380

1.626

46.3

Pyridine

0.71

0.95

330

1.510

115.3

-

1.67

285

1.488

202.0

o-Dichlorobenzene

Disulfide

Methyl acetate

0.60

0.37

260

1.362

N- Methyl

56.3-2-Pyrrolidone
Ethyl acetate

0.58

0.46

256

1.372

77.1

Dimethylsulfoxide

0.62

2.20

286

1.478

189.0

Butyl acetate

-

0.73

254

1.394

126.1

acetic acid

大

1.31

-

1.372

117.9

Methoxyethyl acetate

-

-

254

1.402

144.5

Water

大

1.00

-

1.333

100.0

2-51

<Miscibility of Solvent>Acetic acid
AcetoneAcetonitrile

Immiscibility

BenzeneButanol

Miscibility

Carbon tetrachloride
Chloroform
Cycrohexeane
Cycropentane
Dichloroethane
Dichloromethane
Dimethylformamide
Dimethylsulfoxide
Dioxane
Methyl acetate
Ethanol
Diethyl ether
HeptaneHexaneMethanol
Methyl ethyl ketone
OctanePentanePropanolDipropyl ether
Tetrachloroethane
Tetrahydrofuran
TolueneTrichloroethane
Water
XyleneXylene

Water

2-52

Trichloroethane

In case salt contained solvent, the intermediate solvent which has a goodsolubility to salt, then replace the final solvent.

TolueneTetrahydrofuran

Tetrachloroethane

Dipropyl ether

Propanol

PentaneOctaneMethyl ethyl ketone

Methanol

HexaneHeptaneDiethyl ether

Ethanol

Methyl acetate

DioxaneDimethylsulfoxide

Dimethylformamide

Dichloromethane

Dichloroethane

Cycropentane

Cycrohexeane

Chloroform

Carbon tetrachloride

Butanol

BenzeneAcetonitrile

AcetoneAcetic acid

Replace solvent for a column should be easy soluble chemical against thesolvent pre-used. Make sure that a solubility is good each other. Whenchanging solvents are poor solubility each other, select the intermediatesolvent which has good solubility to each other.

Micro particle elimination
To come in Micro particles such as dust and insoluble, the LC-09XX and thecolumn may be damaged. Such micro particles should be eliminated before
the application.

<Elimination of Micro Particles in Solvent>・With Membrane filter of 0.45μm,
filtrate the solvent using aspirator.
Solvent

・Vapor from the filtering flask
contain organic solvent. To
prevent spill of the organic vapor to

FunnelTo aspirator
Membrane filter

Filtering flask
Filtered solvent

swage, use closed system
aspirator to make vacuum.
Waste water should be disposed
properly according to laws andregulations in the same manner asorganic solvents.

In case clear appearance, some time the solvent contain micro particles,
so make sure to eliminate the micro particles.
Solvent component will be change while the vacuum-filtration. Keep thecondition of the filtration time and vacuum condition. Keep the vacuum
degree and time in constant.

2-53

<Micro Particle Elimination in Sample Solution>・With Membrane filter of 0.45μm, filtrate thesample solution using pressure of asyringe.
・If the sample solution is more than 100ml
do the same elimination of the solvent
following the instructions of the eliminationof micro particles in solvent above

Syringementioned.
Disposable Membrane Filter

Filtered Sample

The sample may contain the micro particles. Make sure to eliminate themicro particles.

Needle port cleaning
The overflowed sample solution may contaminate the needle port of theinjector when injecting the sample with the manual injector.
After injecting the sample with the manual injector, be sure to rinse andclean the needle port described as follows.
・Remove the Syringe Needle from the syringe
and attach Needle Port Guide.
・Suck pure solvent in the syringe and clamp theneedle port guide on the needle port of themanual injector.
・Set the manual injector knob at the [INJECT]
position to perform needle port cleaning.
・Repeat 3 times this cleaning.

2-54

Index(Each number indicates a page number within "Volume 2 General Information Manual". Please refer tothe actual page number "2-*".)

Micro Particle Elimination in Sample［

Ｅ[MAXIMUM SET] Key · 20, 40

Elimination of Micro Particles in Solvent ·Solution · 54
MINSETVAL · 20
Miscibility of Solvent · 52

[MINIIMUM SET] Key · 20

53, 54[OK] Key · 17, 19, 20, 30

ELSE · 26, 46

Ｎ

Ａ

Ｆ

Needle port cleaning · 54

Arrow Keys · 17, 19, 20, 22, 23, 26, 42, 44

Flow Channel Map · 11

Non-diffusion Function · 8, 10, 36

For good Recycling efficiency · 37

Ｂ

Null Glass Adj. Hole · 5

Fraction Nozzle · 3, 4, 7, 12, 26, 27, 46
FRACTION Unit · 26

Brightness Check · 23

Note of good Recycling Separation · 34Ｏ

FROM COLUMN Connector · 5
Operation Panel · 1, 3, 8, 9, 14, 15, 28

Ｃ

Ｌ

Output Port · 6Overlap Trouble · 35

CLEAN UP · 17, 25, 28, 29, 30
Column Holder · 5
Column Reject Valve · 3
Contamination Trouble · 36
Cut Filter Exchanger · 5

LCD Display · 16, 19, 20, 21, 22, 28, 29, 30,
40, 42, 44

Ｐ

Limit of Setting Value · 41
Locking Pin · 43

Power Switch · 4, 6

Luer-lock Syringe · 30

Pre-Column Holder · 5
Pump Drain Out · 7, 30

Ｄ

Ｍ

Pump Drain Valve · 3, 7
Pump Head · 4, 28

Degassing with ON-LINE DEGASSER ·48
Degassing with Ultra Sonic Cleaner · 49,
50

Manual Injector · 5

PUMP Unit · 18

Manual Injector Valve · 3
Manual Sample Injector · 7, 8, 9, 28, 38, 54

Ｒ

Maximum Discharge Pressure · 8
MAXSETVAL · 20

Recorder Signal Range Adjustment · 22,
24, 42, 44

2-55

RECYCLE Unit · 27

Siphon · 3, 4, 7, 12, 26, 27, 30, 46

Recycling Separation · 32

Siphon Box · 3, 4, 7, 30

Relation between Peak Separation andSolvent Property List · 51

the number of Recycling · 32

Solvent Suction Attachment · 30

Ｕ
UV Detector · 4, 23, 26, 46
UV DETECTOR · 21, 43
UV Unit · 21, 24

RI Detector · 4, 25

Solvent Table · 4

RI DETECTOR · 21

Specification · 8

RI Unit · 24

Starting PURGE · 25

ＷStarting RECYCLE · 27

Ｓ

WAKE UP · 16
Starting TEMP.C · 25
Wave Length Tuner · 4
Starting/Stopping the Pump · 18

Selecting SIGNAL POLARITY · 25

Wavelength Selection · 43
STATUS Display · 28

Setting DEAD VOLUME · 26
Setting Detector Response Speed · 21,

Ｚ

Ｔ

23
Zero Adjustment Key · 22, 24
Setting Pump Flow Rate · 19

TO COLUMN Connector · 5

Setting Pump Pressure Limits · 20, 40, 41

2-56

VOLUME

3

Volume 3

Installation Manual

Check list of accessories, inspection sheet and shipping data

Contents
Volume 3 Installation Manual
Chapter 1 Place to Setup .................................................. 3-1
Installation Location ................................................................ 3-2
Installation Conditions ............................................................. 3-3

Chapter 2 Pipe Lines/Wiring Connections ........................ 3-4
Mounting Knobs/Serial Number Check ................................... 3-5
Pipe Line Connections ............................................................ 3-6
Wiring Connections ............................................................... 3-10

Chapter 3 Validation after Installation ..............................3-13
Preparation for the validation ................................................ 3-14
Sample for Validation ............................................................ 3-15
Pipe Line and Start Cable Test ............................................. 3-16
Wiring Connections and Valve Validation ............................. 3-18

Preface
This manual is for JAI staff to install the LC-92XX NEXT series
properly.
Please be advised that installation and relocation by users are notsuggested.
The installation and relocation by users self are unwarrantable.
For more details of operation, information, and maintenance of the
instrument, please refer to other volumes, "Daily Operation Manual","General Information Manual", and "Maintenance Manual" on thetabs.
There are checklist of accessories, shipping data and inspection
sheet attached at the end of this chapter.

3-1

Chapter 1

Place to Setup

Preparation to set up LC-92XX NEXT series

3-1Installation Location
Place the LC-92XX NEXT series at the location and environment that satisfy thefollowing conditions for correct operation.
Installation Location
The LC-92XX NEXT requires space at least more than 500 mm (W) x 600 mm (H) x
600 mm (D). Depth may need 750mm for an air vent and power supply parts at theback of the body. For left and right of the body, may need extra 100mm each forenough space.
The table to place the body has to be horizontally stable and capable to hold theweight of the body.
Please prepare an enough space for optional accessories and attachments as well.

Environment･Indoor temperature must be between at 4 to 35 degree (c) with no dew condensation.
･Must not have temperature changes during operation.
･Indoor humidity must be within 25 to 85%.
･Must be well ventilated.
･Must not produce corrosive gas nor be filled with it.
･Should not be exposed to direct sunlight and direct wind.
･Should be supplying the same working voltage without sudden change.
･Must not give tremor and or shock.
･Must not have strong magnetic field interference.
･Should avoid dusts around.

3-2

Installation Conditions
Place the LC-92XX NEXT series at the location that satisfies the following installation
conditions for correct operation.
Power condition
Power voltage:

AC 100V to 240V (within 10% variation voltage)

Frequency:

50Hz or 60Hz

Power supply:

Requires 500VA at minimum and 800VA at maximum.
For additional business machines, prepare enough power
supply.

Electrical sockets: It has a 3 pins plug with a ground connection (Type A). In caseof none ground connection socket or different type of plug,
please be advised to use an extension and/or adapter to makeit 2 pins plug and connect ground terminal.

3-3

3-2

Chapter 2

Pipe Lines/Wiring Connections

Pipe lines/wiring connections of the LC-92XX NEXT series

3-4Serial Number Check<Serial Numbers>･Check out the serial numbers of the body and detectors.

3-5

Pipe Line Connections
Follow the instructions to connect the pipes appropriately to use the LC-92XX NEXT
series.

<Inside of the Front Door><Install the detector(s).>Front Door

1. UV Detector Tray

2. UV Screw

3. RI Detector Tray

4. RI Screws

Drain Valve・ Pull the front door to open.
・ Take out the plastic bag from inside of the body and unscrew all the sealplugs.
・ Draw out the UV detector tray.
・ Place an UV detector on the tray and retain the UV detector by the UVscrews.
・ Push back the UV Detector tray into the body thoroughly.
・ Draw out the RI detector tray.
・ Place a RI Detector on the tray. Try to avoid the drain valve.
・ Retain the RI Detector on the tray by the RI screws.
・ Push back the UV Detector tray into the body thoroughly; make sure theother pipe lines.

3-6

<Pipe Connection><A line: Pump out tee joint – Pump drain valve>A・ Connect the A pipe between the tee joint of thepump out and the pump drain valve.
UVD

Pump

Pump Drain Valve<B Line: Pump drain valve – FROM PUMP union>

・ Connect the B pipe between the FROM PUMP
union connector and the right side of the drain
valve.

UVD

B

FROM PUMP union

Pump Drain Valve<UV detector– TO UV union>
・ Connect a pipe between the UV detector cell (theinlet port / lower part) and the TO UV union.
UVD
TO UV union

Pipe lines set up view

3-7

<UV detector cell (up part) – UV-RI union (IN)><C line: UV-RI union – RI detector (IN)>UVD・ Connect a pipe line between the UV detector cell(up part) and the UV-RI union.

C・ Connect the C pipe between the UV-RI union(lower part) and the RI detector (IN).

RID
UV-RI Union<D line: Valve union behind drain valve – RI (out) ><E line: Manual injector drain port – Manual injector><F line: Pump drain valve – Pump drain port>

UVD

・ Connect the D pipe line between the upper part ofthe valve union behind the drain valve and the RI(out).

・ Connect the E pipe line between the manual
injector and the manual injector drain port of thesiphon box.

Manual Injector
Drain Port
F

D
E

Siphon Box
RID

Pump Drain Port・ Connect the F pipe from underneath the pumpdrain valve to the pump drain port of the siphon
box.

Valve Union(Behind the Pump Drain Valve)

Pipe lines set up view

3-8

<Solvent supply port – solvent suction line (with line filter)><Total waste – a waste reservoir>
Solvent
Supply Port

Solvent
Filter・ Connect the solvent suction line (with line filter) to
the Solvent supply port.

・ Connect a tube from underneath of the siphon box
into a waste reservoir.

Total Waste
Into a waste
reservoir

3-9Wiring Connections
Follow the instructions to connect the wiring appropriately to use the LC-92XX NEXT
series.

<Main Body to AC Power>
・Put the power cable into [AC INLET 100 ～240 V] at the back of the body.

Unavailable

To AC
power

AC INLET
100V

3-10

<Main Body to Integrator (or Recorder)>・Connect an integrator (or a recorder) signal
cable to the output port (16 terminals).

Output Port

Left hand of
the body

16 |15|14|13|12|11|10 | 9 | 8 | 7 | 6 | 5 | 4 | 3 | 2 | 1

Port No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16

Description
UV +Output for an integrator
UV -Output for an integrator
RI +Output for an integrator
RI -Output for an integrator
UV +Output for a recorder
UV -Output for a recorder
RI +Output for a recorder
RI -Output for a recorder
START +START Recycle signal +Recycle signal Collect valve +Collect Valve Siphon counter +Siphon counter -Top of Output Terminal Block

Wire Release
Hole

Wire Insert
Hole・Connect each signal cable to thecorresponding output port as shown in theabove table, by referring the description below.
・To connect, use a flat head screwdriver andput it into a wire release hole. Insert a cable
into a wire insert hole.
・Remove the screwdriver from the wire releasehole and make sure that the wire end islocked tight in the wire insert hole.

*Minus signals at recycle signal(12), collect valve(14), and siphon counter signal(16) are the same. In use of anelectronic device such as JDS series to read several signals at once, joint the outputs with a jumper wire andconnect as one output port.

3-11

<Connect to JDS Series (Sold as optional accessory)><Connect to a JDS signal cable>
・Connect the cable between the D subminiature connector and the JDS signal inputs[H] and [L].
･In use of 2 different detectors, connect anothersignal inputs [H] and [L] to output port + (3)and – (4) as well.
(The left image shows only the UV detector.)<Connect to JDS 25P D sub-miniature connector>

Top of Output Terminal Block

16 |15|14|13|12|11|10 | 9 | 8 | 7 | 6 | 5 | 4 | 3 | 2 | 1･Insert JDS 25P D sub-miniature connector to
the JDS.
･Connect the signal cables to the output portsof the LC-92XX NEXT series according to thenumbers printed on the mark tubes.

･Check all cable connections.
TO JDS*Each signal cable is attached with a mark tubeon which the number is printed.

Check Cable Connection□

Cable connection is completed

3-12

3-3

Chapter 3

Validation after Installation

LC-92XX NEXT series validation after the installation

3-13

Preparation for the validation
The items listed below are required to validate that the LC-92XX NEXT series isinstalled correctly. * 1 items are standard equipped. * 2 items are prepared by JAI staff.
Those without *1 or *2 should be prepared by customer side.
Upon customer's request, we can execute validation in a different way, after mutual
agreement instead of the following standard validation.

・ Solvent: Chloroform or Methanol 500ml or more.
・ Waste container or bottle 3L capacity or bigger.
・ A beaker 100 ml sized or bigger.
・ Sample vial 2 pcs.
・ Toluene Small amount・ An integrator or a recorder. (In case of purchasing our system with NEXT
seriese, JAI staff will set it up at once.)
・ *1 Hamilton syringe 1 pc.
・ *1 Luer lock syringe 1 pc.
・ *1 8 mm wrench spanner 1 pc.
・ *2 A dummy pressure sensor 1 pc.

3-14

Sample for Validation
Prepare the validation sample for the LC-92XX NEXT series.

・ Measure 1.3ml toluene with a hamilton syringe.
・ 0.3ml toluene out of 1.3ml to a sample vial with hamilton syringe.
・ Prepare chloroform or methanol in a beaker.
・ Take out chloroform or methanol from the beaker by hamilton syringe. 20ml ofchloroform or methanol to the sample vial and dilute the toluene.

3-15

Pipe Line and Start Cable Test
Check the pipe line connection and the start cable connection of the LC-92XX NEXT
series.

･Remove the column cover from the right side of the instrument, and connect adummy pressure sensor between [TO COLUMN] and [FROM COLUMN] using awrench.
･Dip the solvent filter into methanol or chloroform. Place the solvent container on
Solvent table located upper shelf of LC-92XX NEXT series.
･Refer to "Chapter 2 General Operation" in "Volume 1 Daily Operation Manual" toturn the power on and execute the [CLEAN UP] operation.
･When [PUMP PURGE] is displayed on the screen during the [CLEAN UP]
operation, check the solvent out from the pump drain out in the repeat injector box.
In the LC-92XX NEXT, you must inhale the solvent by using a solvent suction
attachment at the preparation for the [CLEAN UP] operation.
・ Settings
Settings
Flow Rate

9.0 mL/min

Pressure Limiter[MAXIMUM] 0[MINIMUM] 0

Injector
Detector UV*1

Detector RI

3 ml (3 times injection)
254 nmIn case of pen recorder, RECORDER SIGNAL RANGE x 1
TEMP. CONTROL ON (LED is ON)
SIGNAL POLARITY POSITIVE (LED is ON)
In case of pen recorder ,RECORDER SIGNAL RANGE (Methanol
x 1, Chloroform x 4)

*1 This is the case of the optical pass length of 0.5 mm. With other optical pass length, adjust
the concentration of sample accordingly.

3-16

・ Start Pump.
・ Make sure that the pump pressure is stable.
Check Column Pressure Stability
Column pressure is stable.

□

・ Refer to the page 1-8 at Volume 1 to perform the zero adjustment of thedetector.
・Set up the integrator or recorder.
・Set the acquisition time to 1.5 min with the integrator.
・ Refer to the page 1-10 at Volume 1 to inject the sample.
・Check no leakage from flow line inside of the front door, connectors of fraction
collector and connectors of dummy pressure sensor during the chromatogram
acquisition.

Leak Check
Inside of the front door
Connectors of dummy pressure sensor□□･Repeat the operation three times.
･When using the integrator, check whether 3 chromatograms are obtained, andwhether the peaks are similar in visual. When using the recorder, check that threepeaks are obtained and that they are similar in visual.

Check Chromatogram
Three chromatograms or peaks are obtained
Peaks are similar in visual･Press the [ON/OFF] key in [PUMP] to stop the pump.

3-17

□
□

Wiring Connections and Valve Validation
Check the wiring connections and valve operation of the LC-92XX NEXT series.

・ Refer to "Volume 1 Daily Operation Manual" to enter the following values:
Flow Rate
Pressure Limiter
Injector
Detector UV*1

Detector RI

Settings
9.0 mL/min[MAXIMUM] 0[MINIMUM] 0
SAMPLE 1 ml
254 nmIn case of pen recorder, RECORDER SIGNAL RANGE x 4
TEMP. CONTROL ON
SIGNAL POLARITY POSITIVE
In case of pen recorder, RECORDER SIGNAL RANGE (Methanol
x 4, Chloroform x 8)

・ Turn on the pump.
・ Refer to the page 1-8 to perform the zero adjustment of the detector.
・ Set the acquisition time to 30 min with the integrator.
・ Refer to the page 1-10 at Volume 1 to inject the sample.
・ 10 seconds after the start, move the manual sample injector to the [LOAD]
position, and press
in [RECYCLE] to monitor the chromatogram.
・ Make sure that peak is in recycling.
・

Check of Recycling Chromatogram
Peak is recycling[R] mark appears on the Chromatogram (JDS acquisition)

3-18

□
□

･After checking the recycling peak, press[RECYCLE] operation. Then press

in [RECYCLE] to stop thein [FRACTION] and check that the eluent

is flowing from the fraction nozzle.

Check Collect Chromatogram
Check the collect valve[C1] mark appears on the Chromatogram (JDS acquisition)

・ After checking the collect valve, press□□in [FRACTION] and check that theeluent stops running from the fraction nozzle.
Check Collect Chromatogram
Check the collect valve[D1] mark appears on the Chromatogram (JDS acquisition)･Press the [ON/OFF] key in [PUMP] to stop the pump.

3-19

□
□

VOLUME

4

Volume 4

Maintenance Manual

Maintenance and trouble shooting of LC-92XX NEXT series(except for optional parts)

Contents
Volume 4 Maintenance Manual
Chapter 1 Maintenance of LC-92XX NEXT ........................... 4-1
Replacement of Column ............................................................... 4-2<Installing Column> ............................................................................ 4-2<Removing Column> .......................................................................... 4-5

Cleaning of Solvent Filter ............................................................. 4-6<Cleaning Solvent filter> ..................................................................... 4-6

Maintenance of Manual Injector ................................................... 4-7<Removing Manual Sample Injector> .................................................. 4-7<Replacing Rotor Seal and Stator Face> ............................................ 4-8<Installing Manual Sample Injector> .................................................... 4-9

Maintenance of Pump ................................................................ 4-10
<Replacing Pump Seal>.................................................................... 4-10
<Cleaning Check Valve, Replacing Check Valve Seal> ..................... 4-14

Pipe Connection ......................................................................... 4-18
<Pipe and Connector, Name and Shape> ......................................... 4-18
<Pipe Line Connections> .................................................................. 4-18

Adjustment of RI Detector .......................................................... 4-20
<Optical Alignment of RI Detector> ................................................... 4-20

Manual Cleaning Instruction of Buffer Volume ........................... 4-21
<Manual Cleaning of Buffer Volume> ................................................ 4-21

Chapter 2 Trouble Shooting ................................................ 4-22
Chromatogram Acquisition Error ................................................ 4-23
<Base Line Wander 1> ..................................................................... 4-23
<Base Line Wander 2> ..................................................................... 4-24
<Base Line Drift> .............................................................................. 4-26
<Base Line Drift during Recycling Operation> ................................... 4-28
<Poor Recycling Efficiency> .............................................................. 4-29

Abnormal Pressure .................................................................... 4-30
<Abnormal High Pressure> ............................................................... 4-30
<Abnormal Pressure Fluctuation> ..................................................... 4-31
<Abnormal Low Pressure> ................................................................ 4-33

Abnormal Signal ......................................................................... 4-35
<Fail Power>..................................................................................... 4-35

<No Signal and No Event Mark> ....................................................... 4-35
<UV Detector Abnormal Signal>........................................................ 4-36
<RI Detector Abnormal Signal> ......................................................... 4-36

Error Message ........................................................................... 4-37
<ERROR 01>.................................................................................... 4-37
<ERROR 02>.................................................................................... 4-37
<ERROR 03>.................................................................................... 4-38
<ERROR 04>.................................................................................... 4-38
<ERROR 05>.................................................................................... 4-39
<ERROR 06>.................................................................................... 4-39
<ERROR 07>.................................................................................... 4-39
<ERROR 08>.................................................................................... 4-40
<ERROR 09>.................................................................................... 4-40
<ERROR 10>.................................................................................... 4-40
<ERROR 11>.................................................................................... 4-41
<ERROR 12>.................................................................................... 4-41
<ERROR 13>.................................................................................... 4-41
<ERROR 14>.................................................................................... 4-42
<ERROR 15>.................................................................................... 4-42
<ERROR 16>.................................................................................... 4-42
<ERROR 17>.................................................................................... 4-43
<ERROR 18>.................................................................................... 4-43
<ERROR 19>.................................................................................... 4-44
<ERROR 20>.................................................................................... 4-44
<ERROR 21>.................................................................................... 4-45

Preface
Fundamental maintenance and trouble shooting of LC-92XX NEXT
series are described in this manual.
Refer to the paragraph for your successfully operation. Prevent solvent
leak and electrical shock during the maintenance.
Normally cut off the AC power. Be careful of electrical shock during themaintenance when power switch turn on.

4-1

Chapter 1

Maintenance of LC-92XX NEXT

Description of LC-92XX NEXT series maintenance

4-1

Replacement of Column
If the separation is required with the different type column, replacement of column isneeded.
Surely stop the pump during the replacement of column to prevent solvent spill.
<Installing Column>
Install the column as following.
･Turn the column reject valve to the [REJECT] position.
･Remove the column cover of the LC-92XX NEXT from the chassis.
･Hold the column(s) on the column holder inside of the column cover.
ColumnTO COLUMN Connector

Column Reject Valve

Column CoverColumn Holder
FROM COLUMN Connector

・Replace the solvent which is able to use with the replaced column(s), with
before used solvent, thoroughly in the flow line of LC-92XX.
Before the solvent replacement, test the solubility between replaced solvent
and before used solvent.
Use Auto-cleaning function for the solvent replacement in the LC.
・Connect the pipe on the [TO COLUMN connector].
Do not connect the pipe on the replaced column(s) yet.
・Set the pump flow rate to use with the replaced column(s).

4-2

・Cover the outlet of the pipe with a paper towel or thick paper for absorption of
the over flowed solvent.
And start the pump for purging air in the pipe. Please make sure to protect
the replaced column(s).
If the flow rate is large, solvent may flush and spray. Be careful.
Thick Paper for Absorption

Be careful of thespray.
TO COLUMN Connector

・Once stop pump and remove the seals of the replaced column(s). Connect
the pipe on inlet of the replaced column(s).
・The pipe is connected on [TO COLUMN connector].
Keep the flow of the replaced column(s) according arrow indicator [Flowseal] on the column. The flow of the replaced column(s) is important for thecolumn performance. Do not connect the reverse direction.

FLOW

ColumnInlet

ColumnOutlet↑
FLOW Seal・When the column is jointed series two or more, the air in the jumper pipe
jointed columns also should be purged.
・Joint the jumper pipe on the first column outlet. Do not joint the pipe on thesecond column inlet on this point.
・Cover the outlet of the jumper pipe with a paper towel or thick paper for
absorption of the over flowed solvent. And start the pump for purging air in
the pipe. If the flow rate is large, solvent may flush and spray. Be careful.

Column Outlet
Thick Paper for Absorption

Be careful of thespray.

・Stop the pump.

4-3

・Connect the jumper pipe on the second column inlet. Connect the pipe fromthe column outlet on the [FROM COLUMN connector].
From Column Outlet

FROM COLUMN
Connector

・Make sure that no leakage is found when the pump is started, then close thefront door.

Parts No.
911-001
911-002
911-003911-004
Parts No.
913-001
913-002
913-003
913-004

Pipes for LC-9210 NEXT
Name
Inlet pipe for Preparative Column
Outlet pipe for Preparative Column(twin column)
Outlet pipe for Preparative Column(single column)
Jumper pipe for Preparative Columns
Pipes for LC-9225 NEXT
Name
Inlet pipe for Preparative Column
Outlet pipe for Preparative Column(twin column)
Outlet pipe for Preparative Column(single column)
Jumper pipe for Preparative Columns

4-4<Removing Column>
To remove columns, refer to the instructions below.
・Turn the column reject valve to the [REJECT] position.
・Remove the LC-92XX NEXT column cover (see page 4-2).
・Remove the outlet pipe column. When the Column pressure is high, you
may have dispersed solvent. Be careful.

Remove the column outlet pipe.
Caution the dispersed solvent
after removing.
To FROM COLUMN Connector

・Put the seal plug into the column outlet. Tighten the seal plug 1/4 turns byhand, and then tighten by a wrench. Use a hand for a PEEK-type seal
plug. Too strong tightening may damage the screws or column. Be
careful.
The tip is a pin
type
Seal Plug・Disconnect the column inlet pipe.
・Put the seal plug into the column inlet.
Make sure that the column removed the pipe are tightly sealed without
solvent leakage, keep in cool dark place.
・Connect with the U shape pipe between the [TO COLUMN connector]and [FROM COLUMN connector] of the LC that are removed the pipe
from the Column.
・Close the column cover.
LC-92XX NEXT common part
Parts No.
Name
901-024
Seal plug : for 1.6φ pipe (column seal)

4-5

Cleaning of Solvent Filter
When the pump flow rate is not stable and, when no solvent is supplied at the autocleaning, the Solvent filter may cause clogging of dust or particles.
The Solvent filter is clogged with dirt and dust if the solvent without dust removal is fed
to the solvent reservoir.
<Cleaning Solvent filter>
・Open the front door. Remove the solvent filter connected on the solvent
supply port.
Front Door

Solvent Supply Port

Solvent Filter

・Remove the Solvent filter and pipe from the solvent reservoir, put theSolvent filter and pipe into a beaker filling the solvent.
・Do clean for 10 minutes with the beaker dipped into an ultrasonic cleaner.

・Replace the filter pipe on the solvent supply port.

Parts No.
995-017995-018

LC-92XX NEXT common part
Name
Solvent supply connector (for NEXT)
Gallons bottle pipe (for NEXT)

4-6

Maintenance of Manual Injector
After high concentration or low solubility sample injection, the Stator face, and the
Rotor seal of the Injector may dirt or precipitate with the remained sample.
If you do not wash with the needle port cleaner after the injection the symptom mayoccur. If solvent start leaking from Manual Injector, or if the ghost peaks appear,
Manual Injector may be improved by maintenance.
Rotor seal and Stator face should be replaced every year.
<Removing Manual Sample Injector>
・Turn the column reject valve to the [REJECT] position.
・Remove the Solvent filter and pipe from the solvent reservoir.
・Remove the column cover of the LC-92XX NEXT from the chassis.
・Remove pipes marked 2, 3, 5, 6 (marked by RHEODYNE Co.) from theback of a Manual Injector.
・Disconnect the connector of start signal cable.
1, 4 are connected a Sample loop, and no need of removal during themaintenance.
1

2

3

6: RHEODYNE Mark

4

5

Start Signal Cable

・Loosen two socket set screws side of the manual injector knob at thefront of the LC-92XX NEXT with a hexagonal wrench.
Injector Knob (front)
Socket Set Screws

・Remove the manual injector knob.

4-7

・Remove retaining screws of the Manual Injector.

Retaining Screws of the Manual Injector

・Move apart the Manual Injector from the back of the LC panel.

<Replacing Rotor Seal and Stator Face>
・Remove Stator retaining screws. Remove Rotor seal, Stator ring, Statorface, and Stator face from the Manual Injector body.
Stator Ring

StatorStator Retaining
Screws

Rotor Seal
Stator Face・Install New Rotor seal. Place the Rotor seal to fit the tip of the needle port
tube into the liquid passing hole, and insert pins into the pin holes. Insert
as the mark of RHEODYNE co. on the Rotor seal faced toward Stator
face.
・Install Stator face as 3 pins fit on each hales.
・Insert Stator ring into the Manual Injector body.
・Insert pins of Stator ring fit on the grooves.
・Tighten retaining screws of the Manual Injector tightly.

4-8

<Installing Manual Sample Injector>
・Place the Manual Injector on the original position at the back of the LC
panel, and tighten the Manual Injector by retaining screws.
・Replace and tighten the Manual Injector knob. Check the smooth
movement of Sample injection.
・If the movement of the Manual Injector knob is too hard or too loose,readjust the Manual Injector knob. Loosen two socket set screws side of
the knob again. Readjust and insert the knob as fit perfectly the key into
the groove of the knob.

Key of the Knob
Groove of the Knob

・Readjust the knob to turn. Appropriately check the movement of the knob
and readjust.
・Tighten the retaining socket set screws to install the Manual Injector knob.
・Connect each pipes restored on the back connection port of the Manual
Injector.
・Connect the connector of start signal cable.
・Start Pump and flow solvent using Auto-cleaning function, and check thatno leakage is appeared at any connector. If the leakage is found, tighten
the connector additionally to stop.
・After the Auto-cleaning, Start Pump and flow solvent into the Column topressurize the system and check that no leakage is appeared at any
connector.
・Close the column cover.

Parts No.
996-012996-014996-015996-016

LC-92XX NEXT common part
Name
Variable Injector(7725i)
Gauge needle for Variable Injector
Rotor seal for Variable Injector
Stator face Assembly for Injector

4-9Maintenance of Pump
The pump has the necessary cleaning supplies and replacement parts, after which
period of time.
The pump seal replaced every four months, do cleaning the pump head in same time.
The check valve packing to exchange every six months, please replace with new ones
every two years.
Around the time of consumption may be due to the property and frequency of thesample.
<Replacing Pump Seal>・Turn the column reject valve to the [REJECT] position.
・Remove the Solvent filter and pipe from the solvent reservoir.
・Open the LC-92XX NEXT front door.
Pump Unit

Front Door・Pump unit is installed in the upper left in the body.
・Disconnect the pipes A, B, C, D connected to the pump unit. For the pipes B
and C, remove the pipes while fixing the check valve cases with a wrench.
While fixing the check valve case with a
10 mm wrench, remove the upper
screw with a 8 mm wrench.

B

C

Pump Head

Turn by hand.
If the screw is hard,cover the screw withpaper and turn by anipper.

A

4-10

D

・Loosen the screw of the pump head with a 2.5 mm hexagonal wrench.
Loosen the screws alternately.
・Pull the pump head. Pull horizontally on the front. The tilted pull may damage
the plunger. Be careful.
Loosen the screws
alternately.

・Remove the pump seal G.
Remove the pump seal G by holding the flange part by hand.
Remove the backup ring left in the main body of the pump unit by holding itwith tweezers (you should also replace the backup ring).
Pump Seal G

Backup Ring(remove with tweezers.)

・Loosen the screw of the pump head guide with a 2.5 mm hexagonal wrench.
Loosen the screws alternately.
・Pull the pump head guide.
*Internal parts may fall out. Be careful not to lose them, especially thestainless ball.
Pump Head Guide
Stainless Ball(keeps on the far
side with grease.)

Loosen the screws
alternately.

4-11

・ Remove the pump seal F attached to the pump head guide by using the seal
removal jig.
Seal Removal
Jig
Use the side withscrew.

Pump Seal F・Clean the pump head. Put Pump head into the beaker. The most frequently
used solvent fills the beaker. Make ultrasonic cleaning for 5 minutes. Empty
the solvent in the beaker. Fill a beaker of methanol. Make ultrasonic cleaningfor 5 minutes. When methanol is non-miscible with used solvent, use ethanol
instead of methanol. Wash the Pump head attached the check valve without
any problems.
・Also wipe around the plunger with BEMCOT or soft gauze. In severe casesof contaminated, moisten the gauze with a strong solvent like as HFIP andwipe the Plunger with the moistened gauze.
・Install the pump seal F on the pump head guide by using the seal removal jig.
Seal Removal Jig
Use the side without
screw.
Keep the pump headstraight to Pump Seal holes
for mounting.

・Install the pump head guide on the pump unit. Insert the pump head guidehorizontally, being careful not to damage the pump seal F with the plunger.
・Tighten the screws of the pump head guide lightly. Tighten two screws on thepump head alternately by 1/4 turns with a 2.5 mm hexagonal wrench totightly fix it.
Install the pump head guide with its holes facing outward.
Hole

Hole

4-12

・Insert the backup ring into the plunger.
・Install the pump seal G on the pump head.
・Tighten the screws of the pump head lightly. Tighten two screws on the pumphead alternately by 1/4 turns with a 2.5 mm hexagonal wrench to tightly fix it.
Be careful not to turn upside down.
・ Connect the pipes A, B, C, D on the pump head. For the pipes B and C,
connect them while fixing the check valve cases with a wrench.
While fixing the check valve case with a
10 mm wrench, tighten the upper screw
with a 8 mm wrench.

B

C

A

D

・Put the Solvent filter into the solvent reservoir. Fill the Pump head withthe solvent to use the [AUTO CLEAN] operation.
・Make sure no leakage. Close the front door.

4-13

<Cleaning Check Valve, Replacing Check Valve Seal>・Turn the column reject valve to the [REJECT] position.
・Remove the Solvent filter and pipe from the solvent reservoir.
・Open the LC-92XX NEXT front door.
Pump Unit

Front Door・Pump unit is installed in the upper left in the body.
・Disconnect the pipes A, B connected to the pump unit. For the pipe B,
remove the pipe while fixing the check valve case with a wrench.
While fixing the check valve case with a
10 mm wrench, remove the upper screw
with a 8 mm wrench.

B

Turn by hand.
If the screw is hard, cover
the screw with paper andturn by a nipper.

A・Remove the check valve inlet case and the check valve outlet case from thepump head. Loosen the check valve to turn anti-clockwise. Be careful not tolose inside parts. Remove the check valve outlet to turn anti-clockwise.
Check Valve Outlet Case

Check Valve Inlet Case

4-14

・The inside parts of the check valve is shown as following figure. Handle as aunit each as "check valve case", "seat", and "check ball", do not confuse theparts each other. Place each unit separately in the beakers.
Check Valve Seat B

Check Valve Seat A
Check Valve Seat A
Check Valve Seat B
Check Valve Inlet Case

Check Valve Outlet Case・Fill each beakers of methanol. Make ultrasonic cleaning for 5 minutes.
・If dirt remains in the visual, remove the dirt with chloroform, toluene, or HFIP
as strong solvent.
・Assemble the check valve. Assemble the check valve as shown below.
Check Ball

Check Valve Case

Flow direction
Seat (Glossy surface)

Assemble glossy surface to acheck ball seat side. Assemble
frosted surface to outside.

Seat (Frosted surface)・Assemble the check valve inlet. Assemble I part on the on the paper. Covergently Assemble part I with the check valve inlet case. Turn over gently bypinching the corners of the paper.
Cover gently Assemble
part 1 with the checkvalve inlet case.
Ⅰ

Turn over gently bypinching the cornersof the paper.

Check Valve Seat A
Seat
Check Ball
Check Valve Case
Check Valve Seat B
Paper

4-15

Low hexagon
head type (4 mm)

・Assemble the check valve outlet. Assemble the check valve Ⅱon the paper,
holding your finger on the top of the parts, and turn over assembled checkvalve gently by pinching the corners of the paper. Put the assembled parts
gently in the check valve case. Turn over the Cover with the assembled parts.

Ⅱ
Check Valve Seat B
Seat
Check Ball
Check Valve Case
Check Valve Seat A
Paper

Turn over gently
by pinching thecorners of thepaper.

Turn over gently bypinching the cornersof the paper.

Turn over gently bypinching the cornersof the paper.
High hexagon
head type (7mm)

・Connect the check valve outlet case into the pump head inlet case, andtighten the case with a 10 mm wrench. The gasket B is fixed tightly into thecase. Remove the case, and make sure that the check valve gasket B
insertion is tight. Then connect the case to the pump head outlet side andtighten it with a 10 mm wrench.
Connect the check valve outlet
case into the pump head inlet
side, tighten the case with a
10 mm wrench, and thenremove the case.

Connect the caseinto the outlet side,and fix it with a 10mm wrench.

Remove the case, and makesure that check valve seat B
insertion is tight and any parts
not fall.

・ Connect the check valve inlet case into the pump head inlet and tighten by awrench anchor.
Connect the case into
the inlet side, and fix itwith a 10 mm wrench.

4-16

・The pipe B is connected to the pump unit as before. Tighten the screw ofpipe B. The Check valve outlet case is fixed by a wrench while the screw istightening.

Fix the check valve case with a 10 mm
wrench, and tighten the screw.

B

Tighten the screw
firmly by hand.

A・Use Auto-cleaning function for the solvent replacement in the Pump head.
・Make sure that no leakage is found. Close the front door.

Parts No.
918-312918-013918-014918-217918-218918-219918-220918-011
Parts No.
928-312918-013918-014918-217918-218918-219918-220928-011

Parts of LC-9210 NEXT (FX-10)
Name
Pump seal G
Check valve inlet (Set)Check valve outlet (Set)Check valve
Valve seat case
Check valve seal A
Check valve seal B
Plunger
Parts of LC-9225 NEXT (FX-25)
Name
Pump seal G
Check valve inlet (Set)Check valve outlet (Set)Check valve
Valve seat case
Check valve seal A
Check valve seal B
Plunger

4-17

Pipe Connection
Attached standard pipe PEEK and Stainless steel pipe is a consumable.
Repeated connection and release of Ferrule will be occurred collapse or bite of thescrews. Increased dead volume and leakage or damage of the screws may cause.
Replace with new ones when the connector repeats three times of lock and release.
PEEK pipe has life. If only organic solvent is used, PEEK pipe early decays. Solvent
leakage or splash may occur from the decayed PEEK pipe.
Please replace a new one a month in continuous operation.
<Pipe and Connector, Name and Shape>A) Male screw

C) Front ferruleB) Back ferruleD) Fitting for PEEK pipe

<Pipe Line Connections>・Insert a Male Screw, a Back ferrule, and a Front ferrule into the pipe.

Front Ferrule
Back FerruleMale Screw

In case of PEEK pipe, insert only D) Fitting for PEEK pipe into the pipe.

4-18

・Insert top of the pipe into a union connector. Pressing the pipe into thebottom of the connector, turn clockwise by hand.
・ Pressing the pipe into the bottom of the connector, turn 3/4 clockwise by awrench.
Union
Turn 3/4 clockwise by awrenchPressing the pipe into thebottom of the connector

In case of PEEK pipe, turn clockwise Fitting for PEEK pipe only by hand, anddo not use a wrench or other tool.

901-010901-011901-023901-030
901-075
901-076901-077

Nut for 1.6φ pipe
Ferrule for 1.6φ pipe (pair)
Male Screw for 1.6φ pipe
SUS 1.6φ pipe
PEEK pipe (o.d.1.5mm, i.d. 0.5mm)
PEEK pipe (o.d.1.5mm, i.d. 0.8mm)
Fitting for PEEK pipe

4-19Adjustment of RI Detector
Optical zero of RI Detector may shift according solvent to use. After turning the powerswitch on, the output value that is displayed on the LCD display immediately after the
auto-cleaning operation indicates the optical position. If the monitoring output is over
±200, the optical alignment is recommended with the re-alignment of the NUL glass.
<Optical Alignment of RI Detector>
・Turning power switch on.
・Press the [CLEAN UP] key to execute the auto-cleaning.
・After the [CLEAN UP] operation, check the RI output value on the LCD display.
・ Remove the Column cover of LC-92XX NEXT from the chassis.

Null Glass Adj. Hole
Column Cover・Insert a blade of a screw driver and turn with slot of the alignment screw.
・While checking the RI output value on the LCD display of the main body, turnthe driver to perform zero adjustment.
・Remove the driver from the hole. Close the Column cover.

4-20

Manual Cleaning Instruction of Buffer Volume
The LC-92XX series has the Reverse flow protection during Recycling Separation as astandard. The Reverse flow protection system has a buffer-volume.
The buffer-volume is automatically cleaned in the CLEAN UP operation. When the LC92XX has stopped in long term, or when the high-viscosity or low solubility sample isinjected, the buffer-volume is contaminated and cannot be cleaned with the CLEAN
UP operation.
If the noise from the buffer volume contamination is remarkable, following Manual
Cleaning of the buffer volume is recommended.
<Manual Cleaning of Buffer Volume>
・Clean the buffer volume with the CLEAN UP operation.
・After the CLEAN UP operation, turn the Pump Drain Valve to the [DRAIN].
・Turn the Column reject valve in the middle between [REJECT] position and[CONNECT] position.
(Refer to below figure.)

REJECT
Turn the valve in the middle between[REJECT] and [CONNECT].

CONNECT・Set the flow rate to maximum. Set 10 mL/min for LC-9210 NEXT and 25
mL/min for LC-9225 NEXT.
・Start the pump, and check that the solvent flows steadily from the pump drain
out in the siphon box. If the flow from the Pump Drain is not appeared, stop
the pump as soon as possible. If setting is abnormal in the Pump Drain Valveor the Column reject valve, the maximum flow may damage the column.
Check the position of two valves, and re-start the pump.
・Press the

key in [RECYCLE].

・One minute later, press
maintenance.

, and then stop the pump to finish the cleaning

4-21

4-2

Chapter 2

Trouble Shooting

LC-92XX NEXT series mechanical failure, error message and remedy

4-22

Chromatogram Acquisition Error
If chromatogram is abnormal, refer to following paragraph and to remedy.
If abnormal chromatograph cannot be remedy, please contact us or our representativeat the end of the book.
<Base Line Wander 1>！Babble in the Reference cell of RI Detector!
・Solvent degassing is recommended.
For details on degassing, refer to the pages describing degassing in"General Information Manual".
Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium.
Check the base line again.
If the wander cannot be remedy, go to the next step <Base Line Wander 2>.

4-23

<Base Line Wander 2>！Babble in the Sample cell of RI Detector, babble in the eluent!
・Check the solvent filter line.
If the babble is found in the solvent filter line, use degassed solvent for theeluent.
For details on degassing, refer to the page 2-48 describing degassing in"General Information Manual".
Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium. Check the base line again.

！Leakage of the UV Detector cell!
・Please contact us or our representative at the end of the book.

！Decay of UV Lamp!
・Please contact us or our representative at the end of the book.

！Leakage of the flow line!
・Check the connectors of pump-injector-column-detector.
If the leakage at the connection of flow is found, tighten the screw further,or replace the new flow line.

4-24

！Loose connection of the Signal Cable!
・Check the connection of the Signal Cable.
If the Loosen connection is found, re-tighten the connection in proper.

！Decay of Pump seal!
・Check the pump head.
If solvent leakage is found under the pump head, refer to Maintenance of
Pump the page 4-10 of "Maintenance Manual" (this manual) for replacementof the pump seal to replace the pump seal if need.

4-25

<Base Line Drift>！Environmental influence of the Laboratory!
・Check that wind hit the LC chassis directly, or that there is a temperaturechange in the laboratory.
RI Detector is sensitive to fluctuation of temperature.
Fluctuation of temperature may cause the base line drift of the RI Detector.

！RI Detector temperature controller works not enough!
・Make sure that the time is over 30 to 100 min after the CLEAN UP operation.
Wait that the temperature controller become stable.

！Solvent replacement is not enough!
・Wait that the Column become stable.

！Contamination of the column is continued!
・Column cleaning is required.
Refer to the column instruction and clean the column if need. In case of
JAIGEL-H series, the solvent replacement is impossible. For the JAIGEL-H
series, the addition of 0.1 - 0.5% tri-ethylamine in the solvent isrecommended. The addition removes and purges the contamination on thepacking material.
In case of using Chloroform, the addition of tri-ethylamine hydrochloride isrecommended in concentration of 50 mM/L.
Before cleaning, test that the sample does not re-precipitate in the added
solvent, and then do the column cleaning with the solvent.

4-26

！Leakage of the flow line!
・Check the connectors of pump-injector-column-detector.
If the leakage at the connection of flow is found, tighten the screw further,or replace the new flow line.
！Decay of Pump seal!
・Check the pump head.
If solvent leakage is found under the pump head, refer to Maintenance of
PumpMaintenance of Pump the page 4-10 of "Maintenance Manual" (thismanual) for replacement of the pump seal to replace the pump seal.

！Leakage of the UV Detector cell!
・Please contact us or our representative at the end of this manual.

！Decay of UV Lamp!
・Please contact us or our representative at the end of the book.

！Contamination of the RI cell!
・Make sure that the UV Detector signal has no noise.
If base line drift cannot be remedied yet, RI detector cell might be dirty.
The RI detector cell is built-in. Customers cannot disassemble and wash the
RI detector cell. Please contact us or our representative at the end of the
book.

4-27

<Base Line Drift during Recycling Operation>

050

Rt (min)

050

100 Rt (min)！Contamination of the buffer volume!
・Clean the buffer volume with the CLEAN UP operation.
When the LC-92XX is started and finished, clean the buffer volume with the
CLEAN UP operation.
When the CLEAN UP is neglected, the base line drift may occur from thesecond-cycle of the recycle operation.

！Not suitable selection of the column!
・When the selection of the column against the sample is not suitable, the baseline is drifting upward in the Recycling operation. Select suitable column(s)for the sample.

4-28

<Poor Recycling Efficiency>

020(Normal)

40 Rt (min) 0

20

40

60 Rt (min)(Poor)！Diffusion of the Recycled Sample in the Sample loop!
・When multiple times Recycling is done, turn over the knob to LOAD toeliminate the sample loop from the flow line because the recycled sample
diffuse in the sample loop.
When you want to use the manual sample injector to execute the recycling
separation, flow the solvent of 3 mL or more for LC-9210 NEXT or 10 mL or
more for LC-9225 NEXT after the sample injection, and then turn the manual
injector knob to the [LOAD] position.

！Not suitable separation conditions!
・When the selection of the column and solvent against the sample is notsuitable, Poor Recycling efficiency appears in the Recycling operation.
Select suitable column(s) and solvent for the sample.

4-29

Abnormal Pressure
Abnormal pressure may occur from decayed flow parts, sample property, or handlingerror. Refer to following paragraph and to remedy the abnormal pressure.
When the abnormal pressure cannot be remedy, please contact us or our
representative at the end of the book.
<Abnormal High Pressure>・If the maximum pressure limit is set, an error message screen appears to
inform the emergency stop of the pump.
Check that the [MAXMAM] pressure limit is set to monitoring pressure +2.0 MPa.

！Clogging of the Column or Dirt of the Column Packing!
・Press the [OK] key, and turn the column reject valve to [REJECT] to start thepump.
・If the pressure is significant smaller than before, column clogging may occur.
・Stop the pump.
・Remove the column from the flow line. Turn the Column reject valve to[CONNECT] position. Re-start the Pump and check the pressure.
・If the pressure is significant large without the Column, the clogging may be
between the LC chassis and the Column inlet. Replace flow line part
between the LC chassis and the Column inlet.
・If the pressure is abnormally high with connection of the Column,
replacement of the Column is need.

！High viscosity of the solvent!
・In case using reverse phase column or using high polarity column, thesolvent replacement of the column may occur high pressure. While thesolvent replacement is finished, set the maximum limiter at the suitable value.

4-30

<Abnormal Pressure Fluctuation>
・If the monitoring pressure abnormally fluctuates, the pressure fluctuation may
occur the abnormal chromatogram or decay of the column. Stop the Pump,
check the following items.

！Clogging of the check valve!
・Turn the pump drain valve to [DRAIN].
・Set the flow rate to 5 mL/min for LC-9210 NEXT and 15 mL/min for LC-9225
NEXT, and start the pump.
・Check that the solvent flows steadily from the pump drain out in the siphon
box. If the flow from the Pump Drain is not constant or repeat stop and flow,
the check valve cleaning is required. Refer to the check valve Cleaning at 414page in this manual.

！Leakage of the flow line!
・Check the connectors of pump-injector-column-detector.
If the leakage at the connection of flow is found, tighten the screw further,or replace the new flow line.

！Decay of Pump seal!
・Check the pump head.
If solvent leakage is found under the pump head, refer to Maintenance of
Pump the page 4-10 of "Maintenance Manual" (this manual) for replacementof the pump seal to replace the pump seal.

4-31

！Babble in the Sample cell of RI Detector, babble in the eluent!
・Check the solvent filter.
If the babble is found in the solvent filter line, use degassed solvent for theeluent.
For details on degassing, refer to the page 2-48 describing degassing in"General Information Manual".
Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium. Check the base line again.

4-32

<Abnormal Low Pressure>・If the minimum pressure limit is set, an error message screen appears to
inform the emergency stop of the pump.
Check that the [MINIMAM] pressure limit is set to monitoring pressure - 2
MPa or less.

！Babble in the Sample cell of RI Detector, babble in the eluent!
・Check the solvent filter.
If the babble is found in the solvent filter line, use degassed solvent for theeluent.
For details on degassing, refer to the page 2-48 describing degassing in"General Information Manual".
Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium. Check the base line again.

！Clogging of the check valve!
・Turn the pump drain valve to [DRAIN].
・Set the flow rate to 5 mL/min for LC-9210 NEXT and 15 mL/min for LC-9225
NEXT, and start the pump.
・Check that the solvent flows steadily from the pump drain out in the siphon
box. If the flow from the Pump Drain is not constant or repeat stop and flow,
the check valve cleaning is required. Refer to the check valve Cleaning at 414page in this manual.

！Solvent loss in the Solvent container!
・Make sure that Solvent fills enough in the Solvent container.
・If the Solvent is lost in the container, the Pump occurs emergency stop to
prevent air introduction into the column.

4-33

！Leakage of the flow line!
・Check the connectors of pump-injector-column-detector.
If the leakage at the connection of flow is found, tighten the screw further,or replace the new flow line.

4-34

Abnormal Signal
The decay of the consumable parts or the property and miss-operation, may occur theabnormal signal of the Detector.
When the abnormal signal cannot be remedy, please contact us or our representativeat the end of the book.
<Fail Power>！Disconnection of POWER Cable!
・Make sure the insertion of the POWER Cable at the rear of LC chassis.

<No Signal and No Event Mark>！Disconnection of SIGNAL Cable!
・Make sure that the connection of signal cable on the Output terminal block at
the left hand of LC.
Check the input cable connection as same.

！Failure of the Recorder or Integrator!
・Please carefully read the instruction manual for your attachment.

4-35

<UV Detector Abnormal Signal>！Dirt in the UV Detector cell and/or Leakage of the cell!
・If the solvent leaks from the UV detector cell, the chromatogram may cause
fluctuation of base line and the sensitivity loss may cause. In the Recycling
separation, extreme low peaks may appear with the leakage.
If above chromatogram is obtained, please contact us or our representativeat the end of the book.
！Decayed UV Lamp!
・If the UV Lamp decays, the chromatogram may cause fluctuation of base line.
The zero balance of the baseline may not achieve. or the sensitivity loss may
cause. If above symptoms appear, please contact us or our representative at
the end of the book.
！Selected UV wave length is not suitable for the sample!
・Each sample has suitable UV wave length for detection.
Select suitable UV wave length for the sample.

<RI Detector Abnormal Signal>！Babble in the Reference cell of RI Detector!
・Solvent degassing is recommended.
For details on degassing, refer to the page 2-110 describing degassing in"General Information Manual". Do the CLEAN UP operation with degassed
solvent and wait that base line becomes stable after the column equilibrium.
Check the base line again.
！RI Detector is out of optical alignment!
・If RI Detector is extremely out of optical alignment, electrical zero may notachieve or the RI signal causes full- scale in tight range.
Refer to p.4-20 described RI Detector Adjustment, and do re-alignment of RI
Detector.
4-36

Error Message
If a failure or wrong operation occurs in the LC-92XX NEXT, an error message
appears on the LCD display. At the beginning of the error message, "ERROR" isdisplayed followed by a number. Based on this number, perform the following
operations to resolve the cause of failure. If the error message appears again, pleasecontact us or our representative at the end of the book.
<ERROR 01>< E R ROR 0 1 >PRE SS VA L UE
M A X I M UM < M I N I M UM
PUSH "OK " KE Y

！This error occurs if the minimum pressure limit is set to a value larger thanthe maximum pressure limit when setting them.
・Set them so that the maximum pressure limit is larger than the minimumpressure limit.
<ERROR 02>< E R ROR 0 2 >PRE SS VA L UE
M A X I M UM = M I N I M UM
PUSH "OK " KE Y

！This error occurs if the maximum pressure limit is set to a value equal to theminimum pressure limit when setting them.
・Set them again so that the maximum pressure limit is not equal to theminimum pressure limit.

4-37

<ERROR 03>< E R ROR 0 3 >P U R G E MO D E( WA I T 6 0 S E COND )PUSH "OK " KE Y

！This error occurs if you press one of the [Co/Dr] key in [FRACTION], the[START] key in [CLEAN UP], the [ON/OFF] key in [RECYCLE], and the[FLOW SET] key in [PUMP], during the [PURGE] operation of [RI
DETECTOR].
・These keys are unavailable during the [PURGE] operation. Use them after
the [PURGE] operation.
<ERROR 04>< E R ROR 0 4 >R E C Y C L E O N MO D E( CHANGE R E CYC L E O F F )PUSH "OK " KE Y

！This error occurs if you press one of the [START] key in [CLEAN UP], the[PURGE] key in [RI DETECTOR], and the [FLOW SET] key in [PUMP],
during the [RECYCLE] operation.
・These keys are unavailable during the [RECYCLE] operation. Use them after
the [RECYCLE] operation.

4-38

<ERROR 05>< E R ROR 0 5 >P UM P F L OW L I M I T
PUSH

"OK "

KEY

！This error occurs if you set a value greater than the maximum flow rate of thepump when changing the flow rate.
・Set a value less than the maximum flow rate of the installed pump.
LC-9210 NEXT(FX-10) : 10.0 mL/min
LC-9225 NEXT(FX-25) : 25.0 mL/min<ERROR 06>< E R ROR 0 6 >C L E A N U P I S NO T MAD E
PUSH

"OK "

KEY

！This error occurs when normal cleaning is not performed in three minutes
during the buffer tube cleaning of the [CLEAN UP] operation.
・If the error is not solved, please contact us. Followings are possible causes ofthe error:
1. The sensitivity of the sensor of the buffer tube is too low.
2. The buffer tube is very dirty.
The error will be solved by replacing these parts.
<ERROR 07>< E R ROR 0 7 >B E YOND T H E L I M I T
PUSH

"OK "

KEY

！This error occurs if you set a value greater than or equal to 50.1 MPa in the
maximum or minimum pressure limit setting screen.
・Set each pressure limit to a value less than 50.0 MPa.

4-39

<ERROR 08>< E R ROR 0 8 >T H E P UM P I S
NO T OP E R A T I NG
PUSH "OK " KE Y

！This error occurs if you press the [Co/Dr] key in [FRACTION], the [ON/OFF]
key in [RECYCLE], or the [PURGE] key in [RI DETECTOR] when the pumpis stopped.
・Press the [ON/OFF] key in [PUMP] to start the pump, and then press thesekeys.
<ERROR 09>< E R ROR 0 9 >T U R N O N T H E P UM P
WH E N U S I NG R E C Y C L E
PUSH "OK " KE Y

！This error occurs if you press the [ON/OFF] key in [PUMP] during the[RECYCLE] operation.
・Stop the [RECYCLE] operation, and then stop the pump.
*The pump is stopped when ERROR 09 is displayed.

<ERROR 10>< E R ROR 1 0 >P R E S S OV E R L I M I T( CL E AN UP )PUSH "OK " KE Y

！This error occurs when the pressure exceeds 1.5 MPa during the [CLEAN
UP] operation.
・Check that the column reject valve is located at the [REJECT] position.

4-40

<ERROR 11>< E R ROR 1 1 >T U R N O N T H E P UM P
WH E N US I NG I N J E C T I ON
PUSH "OK " KE Y

！This error occurs if you perform the injection operation using the manual
sample injector (INJECT->LOAD->INJECT) when the pump is stopped.
・Start the pump before performing the injection.
<ERROR 12>< E R ROR 1 2 >I NVA L I D
OP E RA T I ON
PUSH "OK " KE Y

！This error occurs if you press an irrelevant key when setting a function, or if
you press a key for setting a function when the function is running.
・This error occurs in the following situations:
1. When you press a setting key ([MAXIMUM], [MINIMUM], or [RANGE]),
while fraction collection is being executed by the [Co/Dr] key in[FRACTION], or while the solvent is running through the dead volume.
2. When you press the [Co/Dr] key in [FRACTION], while you are setting thefunction by using the setting keys ([MAXIMUM], [MINIMUM], [RANGE], or[DEAD VOL SET]).
After the operation finishes or the setting is completed, press the key.
<ERROR 13>< E R ROR 1 3 >T H E P UM P I S
OP E RA T I NG
PUSH "OK " KE Y

！This error occurs if you press the [START] key in [CLEAN UP] when thepump is running.
・Stop the pump, and then press the [START] key in [CLEAN UP].

4-41

<ERROR 14>< E R ROR 1 4 >P R E S S OV E R L I M I T( P UM P )PUSH "OK " KE Y
< E RROR

17>

BURN
O U Tto the pressure rise of the pump！This error occurs when an abnormality
due( S T O P T H E P UMP )PUSH "OK " KE Y
is detected.

・As the failure may occur on the parts in the pump, please contact us or our
representative.
<ERROR 15>< E R ROR 1 5 >< E RROR 1 8 >P UMP D O E SOV E R 5 0 . 0 MP a
T ROT A T E( M A X I M UM S E T V A L U EP)U NS O
H "OK " KE Y
PUSH "OK " KE Y

！This error occurs when the monitoring pressure exceeds the specified
maximum pressure limit.
・Refer to the page 4-30 of this manual to solve the problem according< E R ROR 1 9 ><Abnormal High Pressure>.
SOL VE NTE SCAP ED

C HisE displayed.
C K T H E P UM P )*The pump is stopped when ERROR(15
PUSH

"OK "

KEY

< E R ROR

20>

<ERROR 16>< E R ROR 1 6 >F A L L B E L OW 0 1 . 0 M P a( M I N I M UM S E T V A L U E )PUSH "OK " KE Y

！This error occurs when the monitoringF R A C Tpressure
I O N MO falls
D E below the specified
CO L L E C T → D R A I N
minimum pressure limit.
PUSH "OK " KE Y
・Refer to the page 4-33 of this manual to solve the problem according<Abnormal Low Pressure>.
*The pump is stopped when ERROR 16 is displayed.
< E RROR 2 1 >B E YOND T H E L I M I T( M A X M E MO R Y : 3 3 )PUSH "OK " KE Y
4-42

<ERROR 17>< E R ROR 1 7 >B U R NOU T( S T O P T H E P UM P )PUSH "OK " KE Y

！This error occurs when the temperature around the pump control board rises
very high (over 60℃).
・Stop the pump for 30 minutes, and then start the pump again. If the error
occurs again, please contact us or our representative.
*The pump is stopped when ERROR 17 is displayed.

<ERROR 18>< E R ROR 1 8 >P UM P D O E SNO T RO T A T E
PUSH "OK " KE Y

！This error occurs if abnormal rotation is detected at the pump driving partwhen the pump is running.
・As the failure may occur on the parts in the pump such as board or sensor,
please contact us or our representative.

4-43

<ERROR 19>< E R ROR 1 9 >SOL VE NT E SCAP ED( C H E C K T H E P UM P )PUSH "OK " KE Y

！This error occurs if leakage is found around the suction pipe of the pump.
・Followings are possible causes of the error:
1. Check that the connectors such as check valve and PEEK screw aretightened additionally.
2. The leakage is caused by secular change of the pump seal. Refer to thepage 4-10 in this manual to replace the pump seal.
3. Remove the leakage sensor, wipe the liquid out of the sensor, and checkthe LED changes from red to green.
If the error is not solved, please contact us or our representative at the end of
the book.
<ERROR 20>< E R ROR 2 0 >F R A C T I O N MO D E
CO L L E C T → D R A I N
PUSH "OK " KE Y

！This error occurs, if you press the [PURGE] key in [RI DETECTOR], the[FLOW SET] key in [PUMP], or the [START] key in [CLEAN UP], whilefraction collection is being executed by the [Co/Dr] key in [FRACTION].
・Press the [Co/Dr] key in [FRACTION] so that the eluent flows from the siphon,and then press the desired key.

4-44

<ERROR 21>< E R ROR 2 1 >B E YOND T H E L I M I T( M A X M E MO R Y : 3 3 )PUSH "OK " KE Y

！This error occurs if you press the [Co/Dr] key in [FRACTION] 33 times
continuously.
・If you press the key continuously while the solvent is running through thedead volume, the press timings are stored, and accordingly the valve works.
33 times is the limit that can be saved while running through the deadvolume. So do not press the [Co/Dr] key continuously over 33 times.

4-45

CUSTOMER SERVICE
If you have any questions, or something is wrong with Recycling Preparative HPLC, pleasecontact to the following.

208 Musashi, Mizuho, Nishitama, Tokyo 190-1213 Japan
Japan Analytical Industry Co., Ltd
Engineering Department
TEL:+81 42-557-2332
FAX:+81 42-557-0894

Order for Accessories / Supplies
Order for accessories / expendable supplies, please contact to the following.

208 Musashi, Mizuho, Nishitama, Tokyo 190-1213 Japan
Japan Analytical Industry Co., Ltd
Operation Department
TEL: +81 42-557-2331
FAX: +81 42-557-1892

Japan Analytical Industry Co.,

LC-92XX NEXT Series User's Manual (1.3 Edition)
January 31, 2012

Ltd.