Cell size

Concentration

Diffusion and Catalysis Rate

1. Bacteria (E. coli): ≈0.7-1.4 mm diameter,≈2-4 mm length, ≈0.5-5 mm3 in volume;108-109 cell/ml for culture with OD600≈113. Concentration of 1 nM in:E. coli is ≈1 molecule/cell;
Hela ≈1,000 molecules/cell

2. Yeast (S. cerevisiae): ≈3-6 mm diameter,≈20-160 mm3 in volume

14. Characteristic concentration fora signaling protein ≈10 nM-1mM

21. Diffusion coefficient for an “average”
protein: in cytoplasm D≈5-15 mm2/s à≈10 millisec to traverse an E. coli à≈10 s
to traverse a mammalian (Hela) cell; small
metabolite in water D≈500 mm2/s

3. Mammalian cell volume: 100-10000 mm3;
Hela: 500-5000 mm3 (adherent on slide≈15-30 mm diameter)

15. Water content: ≈70% by mass; Generalelemental composition (dry weight) of
E. coli: ≈C4H7O2N1; Yeast ≈C6H10O3N1

Length Scales Inside Cells

16. Composition of E. coli (dry weight):≈55% protein, 20% RNA, 10% lipids,
15% others

4. Nucleus volume ≈10% of cell volume
5. Cell membrane thickness ≈4-10 nm
6. “Average” protein diameter ≈3-6 nm

17. Protein conc. ≈100 mg/ml=3 mM. 106-107
per E. coli (depending on growth rate);
Total metabolites (MW<1kD) ≈300mM

7. Base pair: 2 nm (D) x 0.34 nm (H)Energetics

8. Water molecule diameter ≈0.3 nm

18. Membrane potential ≈70-200 mV à2-6 kBT per electron (kBT≡thermal energy)

Division, Replication, Transcription,Translation & Degradation Rates
at 37°C with a temperature dependence Q10 of ≈2-3

9. Cell cycle time (exponential growth in richmedia): E. coli ≈20-40 min; yeast 70-140min; human cell line (Hela): 15-30 hours
10. Rate of replication by DNA polymerase
E. coli ≈200-1000 bases/s;
human ≈40 bases/s. Transcription byRNA polymerase 10-100 bases/s
11. Translation rate by ribosome 10-20 aa/s
12. Degradation rates (proliferating cells):
mRNA half life < cell cycle time;
protein half life ≳ cell cycle time

19. Free energy (DG) of ATP hydrolysis underphysiological conditions≈40-60 kJ/mole à ≈20kBT/molecule ATP;ATP molecules required to make an
E. coli cell ≈10-50×109
20. DG0 resulting in order of magnitude
ratio between products and reactants
concentrations:≈6 kJ/mol ≈60 meV ≈2 kBT
Useful biological numbers extracted from theliterature. Numbers and ranges should only serve as“rule of thumb” values. References are in the online
annotated version at the BioNumbers website.
Consult website and original references to learn
about the details of the system under study including
growth conditions, method of measurement, etc.

22. Diffusion limited on-rate for characteristic
protein ≈108-109 s-1M-1 à for a protein
substrate of concentration ≈1mM thediffusion limited on-rate is ≈100-1000 s-1
thus limiting the catalytic rate kcat
Genome sizes & Error Rates
23. Genome size: E. coli ≈5 Mbp;
S. cerevisiae (yeast) ≈12 Mbp;
C. elegans (nematode) ≈100 Mbp;
D. melanogaster (fruit fly) ≈120 Mbp;
A. thaliana (arabidopsis) ≈120 Mbp;
M. musculus (mouse) ≈2.5 Gbp;
H. sapiens (human) ≈2.9 Gbp;
T. aestivum (wheat) ≈16 Gbp
24. Number of protein-coding genes:
E. coli ≈4,000;
S. cerevisiae ≈6,000;
C. elegans, A. thaliana, M. musculus,
H. sapiens ≈20,000
25. Mutation rate in DNA replication≈10-8-10-10 per bp
26. Misincorporation rate:transcription ≈10-4 per nucleotide;translation ≈10-3-10-4 per amino-acid

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