Measurement of36Cl and 129I in Water$1--
6XPPDU\ RI 0HWKRG Chlorine-36 and Iodine-129 are separated and measured from up to 500mL aliquots of water.
Samples are adjusted to 0.1-1.0M H2SO4 and 0.1M SnSO4 is added to ensure reduction of any oxidized species tochloride (Cl-) and Iodide (I-). The CL resin is prepared by loading with Ag+ cations which will facilitate the retention of Cland I-. The sample is loaded onto the CL Resin, the column is rinsed with deionized water, and Cl- is recovered using
0.1M ammonium thiocyanate (NH4SCN). If iodine determination is also required, the resin is the rinsed with 0.1% NaOH
and then I- is recovered using 0.35M sodium sulfide (Na2S). 36Cl (t1/2 = 3.01E5 years, Emean = 251keV, Emax =709.55keV, abundance = 98.1% ) and 129I (t1/2 = 1.57E7 years, Emean = 40.03keV, Emax = 149.3keV, abundance =100% ) can then be measured using liquid scintillation counting.

5HDJHQWVCl Resin Bulk (CL-B50-A or CL-B50-S)*-orCl Resin Prepacked 2mL columns (CL-C50-A)
Deionized Water
NaOH*If packing own columns for
H2SO4
gravity flow, then use CL-B50-A.
SnSO4
If using vacuum assisted flow,
Na2S
then use CL-B50-S.
NH4SCNAgNO3 (or Solution of 10mg/mL Ag+)
Liquid Scintillation Cocktail(TXLSPHQW
Centrifuge tubes - 50mL
Liquid scintillation vials, 20mL glass
Liquid scintillation counter
Calibrated pipets and disposable tips
Appropriately sized glass beakers and flasks
Analytical balance
Filter funnel and paper
Optional: Empty columns and frits for packing own columns6DPSOH3UHSDUDWLRQ
Up to 500mL of water sample in glass beaker.

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Weigh 10 grams CL Resin into a 250mL flask.
Dissolve 0.65 grams AgNO3 in 100mL 1M H2SO4.
Add Ag/H2SO4 solution to resin and Mix for 2 hrs.
Filter CL Resin and rinse twice with 1M H2SO4.
Slurry resin in 100mL 0.1M H2SO4 and packinto the appropriate sized column.
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Slurry resin in 100mL 0.1M H2SO4 and packinto the appropriate sized column or obtain
prepacked 2mL column of CL Resin.
Rinse 2mL column with 10mL 1M H2SO4.
Dissolve 0.65 grams AgNO3 in 100mL 1M H2SO4.
Load 2 mL of solution onto each 2mL column.
Wait 2hr for Ag to be completely absorbed.

Adjust to 0.1-1.0M H2SO4.
Add 1 mL of 0.1M SnSO4 per 50mL of sample.
Mix sample well.

Rinse 2mL column with 10mL 1M H2SO4.

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1) Load Sample onto 2mL CL Resin column at 2mL/min.
2) Rinse 2mL CL Resin column with 10mL 0.1M H2SO4.
3) Rinse 2mL CL Resin column with 10mL deionized water.
4) Strip 2mL CL Resin into 20mL LSC vial with 5mL
0.1M NH4SCN to recover chloride.
5) Rinse 2mL CL Resin column with 10mL 0.1% NaOH.

6) Strip 2mL CL Resin column into a 2mL LSC vialwith 5mL 0.35M Na2S.
7) Add 15 LSC cocktail to each LSC vial.
8) Count 36Cl and 129I samples by LSC.

Decontamination Factors (DF)for Chloride and Iodide Frations
AnalyteDF Cl- fraction
DF I- fraction
Ba>1000>600
Cd>6900>7700
Cu>210>190
Mn>210>370
Ni>170>320Pb>300>720
U>1900>200
60

CoCs>320>150>320>150

90

>180
N/A
>420>160>160
N/A

137
90

Sr/ Y
36Cl
129
I

Retention of Cl and Ion CL Resin from 1M H2SO4Analyte
Dw36

Cl
1600
I
1980
Chloride capcity: 4 mg / 2mL column
Iodide capcity: 15 mg / 2mL column
129

5HIHUHQFHV1) A. Zulauf, S. Happel, M.B. Mokili, A. Bombard, H. Jungclas, “Characterization of an extraction chromatographic resin
for the separation and determination of 36Cl and 129I.” J. Radioanal. Nucl. Chem. 286(2), 539-546 (2010).
1955 University Lane Lisle, IL 60532, USA+1 (630)963-0320

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