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Converting Methods fromGravity Columns to Cartridges

6XPPDU\ Performing separations using Eichrom 2 mL
pre-packed cartridges on a vacuum box system offers
many advantages over gravity flow columns, including
faster flow rates, improved chromatographic resolution,and the ability to stack multiple cartridges and measure
multiple analytes from one sample aliquot. Converting
separations methods from gravity flow columns to Eichrom
cartridges is normally simple:

2 mL Cartridge
50-100 mm

2 mL Column 100-150 mm

RackLiner1) Obtain a 12-Hole or 24-Hole vacuum box, vacuum
pump and tubing.

5) Sample aliquots may be collected in individual 50 mL
centrifuge tubes or collectively in the vacuum box liner.
Reservoir

Outer Tip

InnerTube

2) Add cartridges appropriate for the separation.
3) Run separation procedure using the same solutionsand volumes as the column method, adjusting thevacuum to achieve the optimal flow rate:- 1 to 2 mL/min for sample load and rinses.
- 1 mL/min for stripping steps.
4) Drawing air through the cartridges for a short time(<5-10 minutes) between elution steps will notadversely impact the separation. Some samples will
run faster than others.

6) Change cartridge reservoirs and inner and outer tips
prior to elution of each analyte to improve purity.

7) The Eichrom
vacuum box can also
be used for CeF3
microprecipitation
source preparation
using resolve filters.

Table 1. TEVA Resin Performance (2006-2017)*
2 mL Cartridge 2 mL Column
Parameter
50-100 mm
100-150 mm
230
99.3+0.5
99.1 + 0.7
Th % yield239
98.3 + 1.6
98.1 + 1.5
Pu % yield239
0.2 + 0.1
0.2 + 0.1
Pu % impurity in Th
230
0.3 + 0.2
0.3 + 0.2
Th % impurity in Pu*Eichrom Quality Method QA-0213

Table 2. TRU Resin Performance (2006-2017)*
2 mL Cartridge 2 mL Column
Parameter
50-100 mm
100-150 mm
241
99.4+0.4
99.4 + 0.6
Am % yield239
97.2 + 0.6
97.7 + 0.7
Pu % yield239
0.3 + 0.2
0.3 + 0.1
Pu % impurity in Am
241
0.3 + 0.2
0.4 + 0.2
Am % impurity in Pu*Eichrom Quality Method QA-0212

Vacuum Box -12 Hole (AR-12-BOX)
Includes:- Rack for 50mL c-tubes (AR-12-RACK)- Vacuum Gauge (AR-01-GAUGE-PVC)- Vacuum Box Lid (AR-12-LID)- White Inner Support Tubes (25)
- Yellow Outer Tips (25)
- Vacuum Box Manifold Plugs (50)
- Cartridge Reservoir, 10 mL (25)
Optional:- Inner Liner (AR-12-LINER)- Top Support (AR-12-TS)

Table 3. UTEVA Resin Performance (2006-2017)*
2 mL Cartridge 2 mL Column
Parameter
50-100 mm
100-150 mm
230
99 + 399 + 3
Th % yield
233
99 + 499 + 4
U % yield
233
0.2 + 0.1
0.1 + 0.1
U % impurity in Th
230
0.1 + 0.1
0.1 + 0.1
Th % impurity in U
*Eichrom Quality Method QA-0214

Vacuum Box –24 Hole (AR-24-BOX)
Includes:- Rack for 50mL c-tubes (AR-24-RACK)- Vacuum Gauge (AR-01-GAUGE-PVC)- Vacuum Box Lid (AR-24-LID)- White Inner Support Tubes (50)
- Yellow Outer Tips (50)
- Vacuum Box Manifold Plugs (50)
- Cartridge Reservoir, 10 mL (25)
Optional:- Inner Liner (AR-24-LINER)- Top Support (AR-24-TS)

Table 4. Sr Resin Performance (2006-2017)*
2 mL Cartridge 2 mL Column
Parameter
50-100 mm
100-150 mm
Sr % yield
93 + 395 + 3
Ba % impurity in Sr
0.2 + 0.1
0.2 + 0.1
Ca % impurity in Sr< 0.02< 0.02
Y % impurity in Sr< 0.01< 0.01*Eichrom Quality Method QA-0215

Column100-150 mm$GGLWLRQDO(TXLSPHQW- Vacuum Pump (Fisher no. 01-092-25 or equivalent)- Tubing Tygon 1/4 in. I.D., 7/16 in. O.D.
(Fisher no. 14-169-1K, or equivalent)- White Inner Support Tubes (AR-1000-TUBE-PE)- Yellow Outer Tips (AR-1000-OT)- Stopcock, Polycarbonate (12) (AR-12-PC)- 10 mL Cartridge Reservoir (200) (AR-200-RV10)- 20 mL Cartridge Reservoir (200) (AR-200-RV20)
1955 University Lane Lisle, IL 60532, USA

Cartridge
50-100 mm+1 (630)963-0320

www.eichrom.com