THIS IS WHATDISCOVERYLOOKS LIKE
Developing effective treatments andtherapies requires an in-depth
understanding of underlying diseasemechanisms and biological responses, so
it's critical that you see everything yourtissue sample has to show you. But to get
a complete picture can be challenging
with so many complex biologicalinteractions occurring simultaneously.
That's what makes having a streamlined
workflow a powerful solution. The
PhenopticsTM workflow – multiplex
immunohistochemistry staining solutions,multispectral imaging systems, andadvanced image-analysis software – enables
a more comprehensive and specific view
and analysis of biological interactions acrossa digital slide, from the cellular level to themacroscopic tissue architecture, using a
streamlined workflow.

Phenotyping of immune cells and cancer cells within the context of the tumor enables advanced analytics like distance mapping.

PHENOPTICS RESEARCH SERVICES
We take understanding to the next level
Want to test the Phenoptics workflow before bringing the capability inhouse? Let our expert research team generate the results for you.

Better quantification of cellularinteractions may reveal which diseasemechanisms are in play, and help
researchers discover biomarkers that may
eventually lead to better subpopulation
stratification methodologies.
Simply put, that means a better
understanding of biology that drives
disease. And isn’t that the goal we’re all
striving for?

Phenoptics Research Services Laboratory

We follow a detailed staining protocol when working with your precious
samples: Antibody specificity is first confirmed via monoplex with positivecontrols. Then the multiplex panel is tested with the same positive controls,with study samples that you provide – so you’re confident the protocol works
for you. Confirmation of performance levels, including multiplex staining
independence and noninterference are then agreed upon. Analysis begins
with multispectral imaging, providing quantitative spectral unmixing of eachfluorophore signal and tissue autofluorescence, followed by tissue
segmentation and cell phenotyping. This complete workflow enables new
depths of understanding that cannot be achieved with standard chromogenic
monoplex or duplex IHC methods.

UNDERSTANDING STARTS

WHEN YOU PUTCOMPLEXITY INTO CONTEXT

Akoya Biosciences, Inc.
68 Elm Street
Hopkinton, MA 01748 USA
P: (+1) 855-896-8401
www.akoyabio.com

For a complete listing of our US offices, visit www.akoyabio.com.
Copyright ©2018, Akoya Biosciences, Inc. All rights reserved.

Phenoptics™ Research Solutions

UNDERSTANDING

STARTS RIGHT HERE

Combining a powerful multispectral imaging systemwith multiplex IHC staining, and image analysis withinForm® software, the Phenoptics workflow enables
you to identify cellular phenotypes, assess theirfunctional states, and measure spatial relationships.
Want a workflow that enables better understandingof the complex interplay between cells and the tissue
architecture? Let’s get started. . .

IMAGE
Reveal complex biology in asingle tissue section

IMMUNOSTAIN

For a deeper understanding of diseases, you need faster, better
visualization and identification of disease biomarkers. And accelerating thepace of that understanding is the whole idea behind PhenopticTM imaging
instruments. These systems enable you to visualize, analyze, quantify, andphenotype cells in situ, in FFPE tissue sections, and TMAs. Proprietarymultispectral imaging enables you to capture the multiple interactions
occurring between cells because we've carefully unmixed each color
from one another; while also isolating autofluorescence
into its own color channel so you can easily exclude it from your digitalslide analysis. That means you can have confidence in accurately
quantifying the interactions that are really occurring in the biology.

The nine colors of discovery
Opal Multiplex IHC kits make multiplex results
accessible to anyone who works with standard
immunohistochemistry, permitting enhanced visualization
and understanding of complex cellular interactions. WithOpal, you can select antibodies for simultaneous IHCdetection based on performance rather than species.
Opal kits are optimized for reliable spectral unmixing andsimultaneous measurement of three to eight IHC targets,
plus a nuclear stain.
Opal enables you to:

ANALYZE AND
UNDERSTAND

Mantra Quantitative Pathology Workstation:

Opal IHC works with FFPE tissue and is compatible with standard
IHC workflows.

Mantra Quantitative Pathology Workstation• Compact system ideal for getting started with multispectral imaging
or for assay development to free up a higher throughput system suchas Vectra Polaris

Discovery comes withseeing cell-to-cell interactionsOur patented inForm® image analysis software allows
you to accurately visualize, analyze, and quantify
biomarkers in situ in solid tissue. Its powerful unmixing
engine, enables the separation and quantification of
weak and spectrally overlapping biomarkers within cells
and cellular compartments that cannot be identified by
the naked eye. Automated, trainable algorithms permit
detection and segmentation of specific tissues andphenotyping of immune and other cells. Combined withpowerful spatial analysis algorithms in phenoptr andcomplex phenotypic analysis reporting tools inphenoptrReports, these sensitive approaches give you
the confidence to discover indicators of disease anduncover relationships between specific cell types andacross the entire digital slide. Additional benefits include:• Measure three to eight tissue biomarkers simultaneously• Eyepiece for easy visual checking as you go• Use the best primary antibodies, regardless of species —with no cross reactivity• Automated Brightfield and multichannel fluorescence(up to nine colors) capabilities• Identify multiple cell phenotypes while retaining spatial
and morphological context that is lost with bulk
measurements and flow cytometry• Phenochart™ whole slide viewer for slide navigation provides
context for identification of regions of interest within a digitalpathology workflow• Get more information from precious and scarce samples

Vectra 3 Automated Quantitative Pathology Imaging System:• Powerful unmixing algorithm enables identification andseparation of weakly expressing and overlapping signals
from background autofluorescence• Detect and measure multiple weakly expressed and overlapping
biomarkers within a single H&E, IHC, or IF tissue section and in TMAs

• Enables per-cell analysis of H&E, IHC, IF, and RNA-ISH in
FFPE tissue sections and TMAs

• Automatic identification of specific tissue types using integratedinForm® analysis software• Automatically classifies cell phenotypes using machinelearning algorithms

Vectra Polaris Automated Quantitative Pathology
Imaging System:• Automated detection and segmentation of specifictissue types through patented pattern recognition
algorithms

With our Opal Automation IHC kits you can perform
Opal multiplex staining on one of the leading research
automated staining platforms – the BOND RX™ by Leica
Biosystems. Automation provides you with the flexibility tosupport the dynamic demands of translational research.

You can use the best primary antibodies together in multiplex
panels, with no species-based crosstalk.

Vectra 3 Automated Quantitative Pathology Imaging System• Quality, consistency, and reproducibility withevery sample• Premier high-throughput imaging system for detecting and measuringmultiple biomarkers within a single tissue section (up to nine colors)• High-throughput protocol: perform seven-color
immunofluorescence (IF) staining on 30 slides
in 14 hours• State-of-the-art whole slide multispectral imaging for unmixing of up
to seven colors• Simplified, walkaway protocol versus laborious
manual process• Fully automated digital slide scanning that can batch together truebrightfield with multispectral fluorescence

Because you retain spatial cellular context, you get more
information from your precious samples.
Vectra Polaris Automated Quantitative Pathology Imaging System• Flexible data analysis, compatible with many image analysis
software platforms

AB• Pathology Views™ renders immunofluorescence images
as simulated H&E or DAB and hematoxylin, providing
views more familiar to the pathologist• Adaptive Cell Segmentation reliably identifies individual
cell types in densely packed, complex morphologies
regardless of staining heterogeneity and background levels• Simplified whole-slide multispectral imaging workflow
enables inForm analysis across the whole slide, removing
selection biasCDA) Spectral Unmixing; B) Tissue Segmentation; C) Cell Segmentation;D) Cell Phenotyping