ACCESS™ WORKSTATION CONFIGURATION

Access Laboratory Workstation forGenomics

ACCESS WORKSTATION CONFIGURATION | Genomics

A100

ACCESS™ WORKSTATION CONFIGURATIONGenomics

Example of an Access Workstation Configuration

for Genomics Workflows
The Echo Acoustic Liquid Handling technology revolutionizes life sciences by using sound energy to provide highly accurate, fully automated,
non-contact dispensing of fluids. By overcoming traditional liquid handling barriers it lowers the cost of genomics applications in many areas,
including synthetic biology/DNA assembly, gene expression, next-generation sequencing, and single-cell genomics.
Combine the revolutionary performance of the Echo® Liquid Handler with automated plate handling and integrated devices to create walkaway systems tailored for a range of genomics applications. Access systems are modular, flexible, solutions that can scale when needed. Withuse of the Access workstation, Echo Liquid Handlers can be easily incorporated into an integrated system to automate genomic workflows
including, but not limited to, qPCR, PCR, enzyme reactions, DNA/RNA quantification, sample pooling, and normalization.

Inheco ODTC Thermal Cycler

BMG Omega FLUOstar

Roche LightCycler

Thermo Multidrop Combi
Mecour Thermal Tower

System Devices

System Components

 Labcyte Echo 525 or 55X Liquid Handler

 ACC-700 Access Workstation Base System

 BMG FLUOstar multimode reader
 Agilent Technologies PlateLoc Sealer

 ACC-706 2D/1D Barcode Reader

Cytomat C24 Robotic
Incubator
 ACC-708 25-Plate HD Random Access Rack

 Agilent Technologies Microplate Centrifuge • 672, 1536-well plates ACC-710 20-Plate Random Access Racks
 Brooks Automation XPeel Peeler Roche LightCycler 480 or 1536
 Inheco ODTC 384 or 96 Thermal Cycler
 Mecour Thermal Tower- 12mm max height ACC-711 50-Plate Sequential Stacker
 plates
ACC-712 4-Plate Delidder• 504, 96 or 384-well- 18mm max height ACC-714 Base Extension with shelf
 ACC-715
96” Workstation• 310, standard plateswith MicroClime™
LidsTable
 ACC-724 Small Rear Table- 28mm max height ACC-739 Shelf for Small Rear Table

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LABCYTE INC.

Workflows

PCR / qPCR

QC and Normalization• Keep primers and master mix cool using the integrated Mecour
thermal tower• Add PicoGreen dye to DNA samples and use the integrated BMG
FLUOstar to quantify concentration• Combine samples, primers, probes and master mix intominiaturized PCR reactions using the Echo

• Alternatively use Roche LightCycler to perform a qPCR-based
quantitation of DNA samples• Use integrated Inheco On-Deck Thermal Cycler or Roche
LightCycler to perform thermal cycle reactions• Use the Echo Liquid Handler to normalize and pool samples
without creating intermediate dilutions

DNA Assembly

NGS Library Prep

• Rapidly pool DNA fragments and reagents for miniaturized
Gibson or Golden Gate assembly reactions• Transfer DNA samples and library preparation reagents (Nextera,Kapa, NEB, others) into miniaturized reactions• Pool barcodes at a higher density to multiplex more sequencing
libraries

Labcyte Microplates

Sample Storage

Reagent Source• Labcyte Echo qualified microplates are designed for long term
sample storage with minimal dead volume• Labcyte Echo 384 PP microplates and Echo Qualified Reservoirs
are commonly used to contain assay reagents during transfer• Labcyte has partnered with several DNA providers (IDT, Twist,etc.) to provide DNA oligos in Labcyte LDV microplates

Protection• Labcyte MicroClime™ lids protect samples and reagents fromevaporation and contamination• Bio-Rad Auto-Sealing PCR Lid compatible on Access Systemwith Inheco ODTC

CFG-A100-1.0

3ACCESS™ WORKSTATION CONFIGURATIONGenomics

Software Applications
The Access Workstation includes Tempo™ Automation Control Software to adapt Echo software application protocols into fully automated routines.
The Tempo software readily integrates with LIMS systems, analysis software and other tools (DNA design software, for example) to completelyautomate critical genomics workflows.
Many Access Workstation users find the following software packages essential for genomics applications:• Echo® Plate Reformat• Echo® Cherry Pick

Additionally some find that the ability to pre-qualify sample plates prior to transfer on Echo systems greatly improves efficiency. Such users also addEcho® Plate Audit software to their Access Workstations.

Information
For more information, please visit www.labcyte.com/access.

LABCYTE INC.

SALES

1190 Borregas Avenue
Sunnyvale, CA 94089, USA

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OtherToll-free: +1 877 742-6548
Fax: +1 408 747-2010| +1 408 747-2000| +353 1 6791464| +61 39018 5780
| +61 39018 5780
| +1 408 747-2000| info-us@labcyte.com| info-europe@labcyte.com| info-japan@labcyte.com| info-asiapaciﬁc@labcyte.com| info-us@labcyte.com

All product names and brands are properties of their respective owners.
© 2016 Labcyte Inc. All rights reserved. Labcyte®, Echo®, MicroClime®, the Labcyte logo, and Access™
are registered trademarks or trademarks of Labcyte Inc., in the U.S. and/or other countries.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

CAT-A100-1.0
OCT 2016