Conc.

Sialylated HEG1(SKM9-2) HC

NICHIREI BIOSCIENCES INC.
6-19-20, Tsukiji, Chuo-ku, Tokyo 104-8402, JAPAN
Phone: 81-3-3248-2208 Fax: 81-3-3248-2243

Size: 1 ml

Code No.: 498331F
Anti-Human Sialylated HEG1 Mouse monoclonal antibody

INTENDED USE
For further manufacturing use only.

SPECIFICATIONThis antibody reacts with human sialylated HEG1 protein(1). Sialylated HEG1 is a mucin-like membrane protein with many
sialylated O-linked glycans linked to the serine/threonine rich region in the extracellular domain. The antibody recognizes
the sialylated 893 SKSPSLVSLPT903 sequence in the serine/threonine rich region(2). Although the function of sialylated HEG1
has not yet been investigated, it is expressed especially in the cell membrane and cytoplasm of malignant mesothelioma
cells. In malignant mesothelioma, expression was found with ~92% sensitivity and ~99% specificity, which is better
performance than that provided by other major malignant mesothelioma diagnostic markers (1) (3) (4). In normal tissues, very
low expression of sialylated HEG1 is observed, but a few positive cases may be found in capillary endothelium or reactive
mesothelial cells.

REAGENT PROVIDED
Liquid. Concentrate.
Antibody in PBS containing BSA and 0.1% NaN3
For immunohistochemical staining, use 1:200 -1:400 dilution of this antibody

CLONE AND SOURCE
Clone: SKM9-2
Immunoglobulin Subclass: Mouse IgG1
Immunogen: ACC-MESO4 of Mesothelioma
Source: This antibody is derived from culture supernatant of hybridoma

APPLICATIONThis antibody is useful for immunohistochemical staining of human Sialylated HEG1 protein.

USAGE
1. Specimen Pretreatment
Specimen pretreatment of formalin fixed paraffin embedded tissue sections with heat induced epitope retrieval (HIER) isrequired.
2. Incubation Conditions for the Antibody
For 30 to 60 minutes at room temperature.

STORAGE AND HANDLING
1. Store at 2-8°C.
2. Confirm expiration date indicated on the labels before use.
3. Adjust this product to room temperature before use.
4. Refrigerate this product promptly after use.

WARNING AND PRECAUTIONS
1. Before using this reagent, please read these instructions.
2. Do not use reagents after the expiration date.
3. Specimens, before and after fixation, and all other materials exposed to them, should be handled like biohazardous
materials with proper precautions.
4. Inhalation or ingestion of the highly allergic fixative formaldehyde is harmful. Wear protective mask. If swallowed, induce
vomiting. If skin or eye contact occurs, wash thoroughly with water.
5. Organic reagents are flammable. Do not use near open flame.
6. Avoid inhale this product or contact with eyes, mouth, skin and clothing as well.
7. Avoid microbial contamination of reagents as incorrect result may occur.
8. Avoid splashing of reagents or generation of aerosols.
9. Handle this product with attention due to its animal-derived component contained.

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10. Sodium azide is a toxic chemical. The concentration in this product is not classified as hazardous, however, accumulated
NaN3 may react with lead/copper plumbing to form explosive metal azides. To prevent such risk, flush the disposed
reagent with copious amount of water, or dispose this product properly in accordance with applicable rules and regulationsof local and country.

LIMITATION
Tissue staining is dependent on the handling and processing of the tissue prior to staining. Improper fixation, freezing,thawing, washing, drying, heating, or sectioning may produce artifacts or false-negative results. Results will not be optimal
if old or unbuffered fixatives are used, or excessively heated during embedding or during attachment of sections to slides.
False-positive results may be seen due to nonspecific binding of proteins. The use of blocking reagent separately, in somecases the application of blocking reagent containing an irrelevant protein, prior to incubation with the primary antibody, may
be useful for reducing the background. The optimal concentration and incubation time of primary antibodies should bedetermined by manufacturer. In some cases, further dilution of primary antibodies may be required to prevent over-staining,
especially in frozen tissue sections.

REFERENCES
(1)
(2)
(3)
(4)

Tsuji S, et al. HEG1 is a novel mucin-like membrane protein that serves as a diagnostic and therapeutic target for malignant mesothelioma. Sci Rep.
2017 Mar 31;7:45768.
Matsuura R, et al. Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-likemembrane protein HEG1. Sci Rep. 2018 Sep 24;8(1):14251.
Hiroshima K, et al. HEG1, BAP1, and MTAP are useful in cytologic diagnosis of malignant mesothelioma with effusion. Diagn Cytopathol. 2020May 22.
Naso JR, et al. HEG1 Is a Highly Specific and Sensitive Marker of Epithelioid Malignant Mesothelioma. Am J Surg Pathol. 2020 Aug;44(8):11431148.

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