Health Care Promotion
by Lifeceramics（English Abbreviation Version）

Nobuo Suzuki、Kazuko Kita, Shigeru Sugaya,
Takeshi Tanaka, Mei Dong , Xiao-Bo Tong

CONTENTS
1. Research Strategy・・・・・・・・・・・・・・・・・・・・・・・N.Suzuki
2. Risk management of health care by SOS response of a humanbody・・・・・・・・・・・・・・・・・・・N.Suzuki
3. Possibility of liver function promotion by lifeceramics ・・・・N.Suzuki
4.Possibility of renal function promotion by lifeceramics ・・・・N.Suzuki
5. Possibility of suppression of fatty-acid metabolism disorder ・・N.Suzuki
6..Possibility of promotion of clcohol metabolism function by
lifeceramics ・・・・・・・・・・・・・・・・・・・・・・・・N.Suzuki
7. Suggestion of risk management for health care under stressful social
conditions(1) Thought-provoking of saliva testing for construction of streesless
society ・・・・・・・・・・・・・・・・・・・・・・・・ S.Sugaya(2)
Possibilityof
miligationofstressconnditons
bylifeceramics ・・・・・・・・・・・・・・・・・・X.Tong &N.Suzuki
8. Summary of life ceramic effectiveness in a human body ・ ・ ・ ・N.Suzuki
9. Effects of lifeceramic-treated water on rexistance to oxidatibe stress inhuman cells ・・・・・・・・・・・・・・・・・・・・・・・・ K.Kita
10. Ability of lifeceramic-treated water to regulate expression of various
genes ・・・・・・・・・・・・・・・・・・・・・・・・・・ T.Tanaka
11. View---Creation of environmental medical care by application of
Lifeceramics ・・・・・・・・・・・・・・・・・・・・・・・ N.Suzuki

1. Research strategy
N. Suzuki
Search for human SOS physiological functions is tried by creating tools for thereseach from about 40 years ago. Suggestions for resolving problems were obtained due
to findings of effects of radiation exposure at low doses on cultured human cells andhuman bodies. And then research products, educational systems and outreachactivities in Environmental Biochemistry, Chiba University during the past 15 years
are introduced. Our efforts have continued to identify factors related with human SOS
responses based on previous findings of the response phenomenon in Escherichia coli.
The response mechanism in a human body have been found to consist of serum factors
and activation of proteases. The serum factors likely include cytokines, chaperones andtheir binding proteins, each of which intra-cellularly and extra-cellularly supervises
cellular DNA repair and apoptotic functions. According to the stress conditions, thesupervisors could regulate both error-free and -prone mechanisms to control geneticstability.
Due to the above results and their usage we could find out novel physiological effectsof Lifeceramics on human cells and bodies. Thus, our trial and its fruits, presented
here, will prove usefulness of Lifeceramics for human health care promotion..

2.Risk management of health care by SOS response of a humanbody ・・・・・・・・・・・・・・・・・・・・・・・・・・・・N.Suzuki
As one of the first steps op cancer development there is a basesubstitution mutation. In the mutation various kinds of molecules areinvolved. And the molecules’ action is regulated by another molecules as asupervisor. Thus, the supervisors, cytokines, proteases and chaperones,could determine the fate of genes.
On the other hand, mutation is the prime mover of biological evolution.
Therefore, the above supervisor could play an important role as the primemover. All of these possibilities could be found by original research strategy,
so-called human SOS biological science.
3.Possibility of liver function promotion by Lifeceramic ・ ・ ・ ・ ・N.Suzuki
Lifeceramics-treated water was examined whether it plays some roles on
curing liver function disorder. Peripheral blood was obtained fromvolunteers who drank the water on the please. Blood was collected from 11and 4 donors 10 and 30 days after drinking, respectively.
Results indicate the possibility that liver function disorders are cured after
drinking the Lifeceramics-treated water.
4.Possibility of renal function promotion by Lifeceramics・・・・・

N.Suzuki
Blood samples, reported at chapter 3, were used to examine whether
Lifeceramics-treated water is useful for curing disorder of renal function.
Increased levels of urea N and creatinine were descend in 4 volunteers.
5.Possibility of suppression of fatty-acid metabolism disorder ・ ・ ・N.Suzuki
Blood samples, reported at chapter 3, were used to examine whether
Lifeceramics-treated water is useful for curing disorder of fattey-acidmetabolism.
Increased levels of triglyceride was evidently decreased.
6.Possibility of promotion of clcohol metabolism function by
Lifeceramic ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・N.Suzuki
Lifeceramics goods, available at shops, are made of ceramics Powder itself
was examined for its effectiveness on alcohol metabolism. Dr. Dong Mei
tried to estimate alcohol (EtOH) concentration in venous blood from rats
after administration of ceramics powder and alcohol Sake. The
concentration was increased after the administration and showed thehighest from 1 h up to 2 h after that. However, the increased levels werelower at rats with ceramics powder than these at rats without the powder.
In human drinking Lifrceramics-treated water alcohol concentration inrespiration air was increased depending on volume of the water drunk.
7.Suggestion of risk management for health care under stressful socialconditions(1)Thought-provoking of saliva testing for construction of stressless
society ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・S.Sugaya
Test of saliva analyses activity is thought to indicate conditions of theparasympathetic nervous system which responses to various stressors.
The present stressful society needs the test in order to build up thesociety where people can get rid of stress.
(2)Possibilityofmiligationofstressconnditons
byLifeceramics ・・・・・・・・・・・・・・・・・・・X.Tong&N.Suzuki
It is an interesting problem whether we can get rid of stress by drinking
Lifeceramics-treated water. Dr. X. Tong evaluated stressful or nonstressful conditions of volunteers by estimation of amylyses acticity
levels in saliva. The levels were decreased in 11 volunteers among 13
those tested. Therefore, it seems likely that Lifeceramics for drinking isuseful for suppression of stress-building up.
8. Summary of Lifeceramic effectiveness in a human body ・ ・ ・N.Suzuki
Based on the results obtained in the present study the following checkpoints could clearly indicate the possibility of effectiveness of Lifeceramicstreated water on our healthy conditions: TTT for examination of liverfunction, urea N, creatinine, and CPK for examination of renal function, andtriglyceride, total cholesterol and LDL cholesterol for examination of fattyacid metabolism.

9.

Effects of Lifeceramics-treated water on resistance to oxidative stress inhuman cells ...........................................K. Kita
The cellular effects of lifeceramics-treated water on resistance to oxidative stresswere examined using cultured human cells. The cells,cultured in medium preparedwith lifeceramics-treated water, showed increased resistance to hydrogen peroxideinduced cell death. Resistance to X-ray-induced cell death was also slightlyenhanced by the culture with the medium. In contrast, neither capacity to repair
ultraviolet light C (UVC)-induced DNA damage nor resistance to UVC-induced
cell death was enhanced by the culture with the medium. Hydrogen peroxide causes
oxidative stress, and X ray also causes oxidative stress and the direct formation ofDNA damage in human cells, whereas UVC causes direct DNA damage. Therefore,
the present results suggest protective effects of lifeceramics-treated water against

oxidative stress in human cells.
10. Ability of lifeceramic-treated water to regulate expression of various
genes ・・・・・・・・・・・・・・・・・・・・・・・・・・ T.Tanaka
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression bybase pairing specifically with nucleotides in the 3ʼ untranslated regions of target
mRNAs or by directing mRNA degradation . MiRNAs play important roles in cellproliferation, apoptosis, and differentiation . In particular, miRNAs that regulate cellproliferation have been implicated in various cancers. We focused on a few miRNAs
whose expression levels were altered by the addition of Lifeceramics-treated water incell
culture medium. Based on microarray analysis, we focused on two miRNAs (miR-381
and miR-431) whose expression levels were modulated in the cultured humancells.Furthermore, expression levels of GAS1 gene, one of cancer suppressor genes,
were upregelated in the cultured cells. Thus, Lifeceramics-treated water may be usefulfor the suppression of tumor cell growth.

11. View---Creation of environmental medical care by application of
Lifeceramics ・・・・・・・・・・・・・・・・・・・・・・・ N.Suzuki
Based on human SOS biological science, methods to establish
healthy conditions were found, such as Japanese Miso intake.
Furthermore, forest-walking together with drinking Lifeceramicstreated water would be another valuable method. On the other hand,radio-active materials could be eliminated by Lifeceramics
themselves. Thus, Lifeceramics will be applicable for protecting
our health against undesirable environmental conditions.

Authors
Nobuo Suzuki*,***
Kazuko Kita*,
Shigeru Sugaya*,Takeshi Tanaka*,
Masataka Yosida*,***,Mei Dong **,Xiao-Bo Tong*****Department of Environmental Biochemistry, Graduate School of Medicine,
Chiba University, Inohana, Chuo-ku, Chiba 260-8670, Japan**
Department of Forensic Medicine, Hebei Medical University, 050071
Hebei, P.R. China***
Non-profit Organization, Chiba Researchers Network for Health Care
Promotion, Chuo-ku, Chiba 260-8670, Japan****Department of Physiology, Chengde Medical University, Chengde 067000,

P.R. China

Published byNon-profit Organization, Chiba Researchers Network for Health CarePromotion, Chuo-ku, Chiba 260-8670, Japan
2013. 5. 31