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TDR/STR/SEB/ST/03.1

Ethical, legal and social issuesof genetically modified
disease vectors in public health

Darryl Macer1 Ph.D.

1 Director, Eubios Ethics Institute, Japan and New Zealand
Associate Professor, Institute of Biological Sciences,
University of Tsukuba, Tsukuba Science City,
305-8572, Japan
Email: Macer@sakura.cc.tsukuba.ac.jp

TDR/STR/SEB/ST/03.1
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TABLE OF CONTENTSEXECUTIVE SUMMARY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.

INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.1
1.2
1.3

2.

THE GENETIC ENGINEERING DEBATE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1
2.2
2.3

3.

Methods used for control of insect vectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Past attempts at introduction of modified pathogens . . . . . . . . . . . . . . . . . . . . . 9
Development of genetic modification techniques . . . . . . . . . . . . . . . . . . . . . . . .10
Modification of other organisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Modification of human hosts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

ETHICAL AND SOCIAL ISSUES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
4.9
4.10

5.

Growing use of genetic engineering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
International systems for consideration of genetic engineering . . . . . . . . . . . . . . . 7
Codex Alimentarius Commission and the safety of genetically modified food . . . . . . 8

MODIFICATION OF VECTORS AND PATHOGENSFOR PUBLIC HEALTH PURPOSES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1
3.2
3.3
3.4
3.5

4.

Ethics of disease prevention . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Bioethics and biotechnology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Bioethics and molecular entomology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Reducing the complexity of ethical and social issues . . . . . . . . . . . . . . . . . . . . . 13
Intrinsic ethical issues of genetic engineering. . . . . . . . . . . . . . . . . . . . . . . . . . 15
Animal rights concerns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Consent from trial participants. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Consent from broader society on environmental risks . . . . . . . . . . . . . . . . . . . . . 18
Social consensus building and early cessation of trials . . . . . . . . . . . . . . . . . . . . 18
Equality and inequality in access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Ethics of technology choices and knowledge development . . . . . . . . . . . . . . . . . . 20
Intellectual property rights and technology transfer . . . . . . . . . . . . . . . . . . . . . . 20
Inducement to participants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

PUBLIC ATTITUDES TO GENETICALLY MODIFIED ORGANISMS . . . . . . . . . . . . . . 23
5.1
5.2
5.3

Need for public acceptance prior to interventions . . . . . . . . . . . . . . . . . . . . . . . 23
Results of public opinion surveys on genetically modified organisms . . . . . . . . . . . 24
Wide social and legal discussion of biotechnology . . . . . . . . . . . . . . . . . . . . . . . 25

iii

6.

REGULATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.1
6.2
6.3

7.

General basis for regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Cartagena Protocol on Biosafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
National and regional regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

RECOMMENDATIONS FOR KEY AREAS TO ENSURE ETHICAL INTEGRITY IN
THE USE OF GENETICALLY MODIFIED VECTORS AND PATHOGENS . . . . . . . . . . . 31
7.1
7.2
7.3
7.4
7.5
7.6

Establish safety and international standards before field trials . . . . . . . . . . . . . . .31
Information access and local communities as partners . . . . . . . . . . . . . . . . . . . . 32
Group consent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Environmental assessment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
International cooperation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
Further next steps in consultation on ethical, legal and social issues . . . . . . . . . .34

ACKNOWLEDGEMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
ABBREVIATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

iv

EXECUTIVE SUMMARY

C

onsistent with its implicit ethical mandate to reduce human suffering from disease, TDR has outlined a three-pronged effort to develop genetically modified (GM) mosquitoes for control of tropical diseases such as malaria. The approach to genetically modify vectors or their symbionts
and/or pathogens for disease control raises few intrinsic ethical issues; however, important environmental and human health concerns need to be assessed before release of any genetically modified
organism (GMO). Each country needs to decide its own policy guidance for ethical genetic engineeringof microorganisms, plants, animals and ecosystems, and to negotiate with neighbouring countries; thispolicy advice should be the product of open dialogue involving all sectors of society. However, given
the broad acceptance of use of GMOs for specific purposes, as endorsed by specialized international
agencies including the United Nations Development Programme (UNDP) and United Nations Food andAgriculture Organization (FAO), the question is not so much whether to release GMOs but rather how
to release them and what type are safe and effective enough to enter field trials.
Part of the process is for society to set values for consensus on risk assessment. A universal minimal
standard of risk assessment applicable to disease vectors needs to be defined, as diseases cross national and continental borders. In developing model guidelines, this report recommends examining the following issues:• Before field release of transgenic insects, researchers must assess all the scientific and social issues
associated with GM vectors and develop safety precautions to address potential risks.
• The scientific and social risks should be minimized through careful design of the vector system, relevant laboratory experience, and careful choice of the site including considering appropriate socialand cultural factors.
• Even if there are not perceived to be any realistic risks, a procedure for their evaluation should be
set up so that new information can be gathered and interpreted. This procedure may involve establishing a specialized ethical review committee under TDR auspices to offer advice to researchers on
the ethics of projects.
• There should be prior environmental, medical and social studies for site selection, and the mostappropriate site chosen on the basis of these data.
• Information should be exchanged as broadly as possible with community leaders, members of thelocal community, and the mass media.
• Consent should be obtained from the communities involved. Specific mechanisms to obtain individual and group consent need to be developed for public health interventions.
• A contingency plan for aborting a field trial needs to be developed.
• Commitment to the local communities involved in field trials should be made such that they will be
the first beneficiaries of more permanent use of a GM vector should results indicate that this isappropriate.
• Intellectual property concerns should not be barriers to implementing public health measures using
GM vectors or their symbionts and/or pathogens. Prior negotiation, including possible involvement
to allow access to the latest technology, is preferable to confrontation.
• To avoid any suspicion by the public that could result in public rejection of the approach, TDR andmember governments should not involve partners in the projects from any military research establishments.

Ethical, legal and social issues of genetically modified disease vectors in public health1The data should be made available to all in order to benefit from global expertise and develop international consensus. There is a need for an ongoing and active process of ethical analysis, through avariety of forums, and TDR is called upon to take a lead in elaborating and developing ethical andscientific standards for research in this area.

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Social, Economic and Behavioural Research • Special Topics No. 1

1 INTRODUCTION
1.1 Ethics of disease prevention
The ethical mandate of TDR is to improve existing and develop new approaches for preventing, diagnosing, treating and controlling infectious diseases that cause loss of human life. The ethical principlethat lies behind the idea of preventing, treating and controlling disease is that human life is somethingworth saving. Certain principles basic to resolving ethical dilemmas (Engelhardt, 1986; Gillon, 1986)
need to be considered before proceeding to examine the topic of genetic engineering for public health.
The principle that we should love the life given to us (self-love) implies that each person should begiven autonomy (self-rule) to work out how to balance the ethical dilemmas and choices themselves.
The Universal Declaration of Human Rights of 1948 specifically set as a baseline that all human beings
possess equal rights, and should be given a chance to exercise their autonomy. One of the fundamental human rights is a right to health, and working towards giving every person a chance to grow up
free of disease is the ethical foundation of public health. If a person does not possess some basic levelof health, he/she cannot even face many of the choices commonly accepted as normal. Poverty also
restricts the choices of many people (Azevedo and de Moraes, 2002).
Justice simply means that if we want others to recognize our autonomy, we have to recognize theirs
as well. There are at least three different meanings of the concept of justice: compensatory justice meaning that the individual, group, or community, should receive recompense in return for contribution; procedural justice - meaning that the procedure by which decisions about compensation and distribution are made is impartial and includes the majority of stakeholders; and distributive justice meaning an equitable allocation of, and access to, resources and goods. There are ethical questions
about how a society should represent procedural justice when there are major divisions within the society on particular issues. The process of consensus building and reaching common ground may be preferable for many cultures rather than confrontations based on a direct referendum, as is sometimes usedin Switzerland. These issues are discussed below, given the controversies surrounding GMOs.
At present there is great inequality between rich and poor nations in the direction and priorities ofresearch, and in the distribution of and access to benefits that might come from this research. Under
any ethical theory, the presence of diseases that threaten the lives of not just one but more than a billion people worldwide provides a compelling need for efforts to eradicate the diseases. There is wide
diversity in the risks that members of each community face from infectious diseases due to: individual
genetic variation in resistance to infectious disease agents; a person’s nutritional state and immediate
environment; a family’s economic situation with respect to providing barriers to vectors and disease;access to both preventative and therapeutic medicines. These variations can be regarded as a type oflottery. Working towards better global equity is a goal that attempts to even out the lottery that people are born into. This is ethically mandated by Rawlsian justice (Rawls, 1971), which argues thatefforts should be made to minimize the variation in all social factors because no one knows before they
are born into which situation they will be born, so everyone would wish for equal opportunity and equal
exposure to risk. All should have a chance to be born and grow up in an environment free of infectious
diseases, if that can be achieved.
The ethical principle of beneficence supports the development of science and medicine, and its provision to those who suffer. A universal ideal found throughout human history is that it is better to love
doing good things than bad things, and to love our neighbour as ourselves. Humans have used technology in efforts to make their lives easier and better for thousands of years, and the ethical principleof beneficence argues that we should continue to make life better. This ethical principle is based on
the general motivation inside people to love doing good rather than harm, and may be expressed aslove or compasssion (Boyd et al., 1998). Efforts that work for the betterment of others in society havea universal moral mandate.

Ethical, legal and social issues of genetically modified disease vectors in public health3

The ethical principle of non-maleficence, or do no harm, would make us reasonably cautious about
premature use of a technology when the risks are not understood. Recently some have advocated a
total precautionary principle for genetic engineering, which would mean that no technology with morethan 0% risk should ever be attempted (Ho, 1998). This has also entered the Cartegena Protocol on
Biosafety (CBD, 2000).
Because no human action has 0% risk, the principles of both benefit and risk are used to assess technology and are central to any public health programme. Few papers have considered the ethics of public health (Callahan and Jennings, 2002). The basic ethical principles of autonomy, justice, beneficenceand non-maleficence can be applied to help decision-making in a range of bioethical dilemmas in medical and environmental ethics. There is some debate over whether further principles can always bederived from these (Veatch, 1989), and over the precise terminologies in each field (Weed and
McKeown, 2001), but the general consensus is that these four principles are fundamental in a range ofcultures (Beauchamp and Childress, 1994; Tsai, 1999).
In different societies there are debates over whether principlism is the most suitable form of ethicaltheory for decision-making; however, it is the most widely accepted in modern bioethics. The emphasis on individuals may be questioned more in developing countries. There are also theories of ethics
based on community, which argue that individuality, autonomy or rights of a person are not suited tothe community structure of society. Community advocates argue that societies need a commitment togeneral welfare and common purpose, and that this protects members against the abuses of individualism, which can be equated with selfish pursuit of liberty. The question is what community we talkof, whether the individual family, the village, the state, country, region, or global community.
MacIntyre (1984) argued that Aristotle considered local community practices and their corresponding
virtues should have primacy over ethical theory in normative decision-making. These practices include
parenting, teaching, governing, and healing.
Despite the fact that there are a variety of definitions of health, disease, disability, and meaningful
human life, working to alleviate disease and empower individuals to reach their potential are universal goals for the progress of humankind. This report seeks to examine how these goals may be accomplished considering the use of genetic engineering for public health purposes. Before we do this, wewill consider more the ethical theories that people use and the rise of biotechnology.

1.2 Bioethics and biotechnology
Recent developments in biotechnology have made people re-examine the ethics of life. The term
bioethics has emerged as a term to summarize the ethical issues associated with human attitudes tolife, the environment, use of natural resources and biotechnology. Much recent attention has focusedon medical ethics and human health questions, but the concepts of bioethics have also long included
environmental ethics. Bioethics is a broad concept linking many traditional academic fields(Beauchamp and Childress, 1994; Reich, 1995; Macer, 1998, 2002). Central to the concept is recognition of the autonomy of patients and the subsequent need for informed consent in medicine (Ramsey,1970).
There are at least three ways to view bioethics:• Descriptive bioethics is the way people view life, their moral interactions and responsibilities withliving organisms in their life.
• Prescriptive bioethics is to tell others what is ethically good or bad, or what principles are mostimportant in making such decisions. It may also be to say something or someone has rights, andothers have duties to them.
• Interactive bioethics is discussion and debate between people, groups within society, and communities about descriptive and prescriptive bioethics.

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Social, Economic and Behavioural Research • Special Topics No. 1

Developing and clarifying descriptive and prescriptive bioethics, usually through a process of social
interaction, allows us to make better choices, and choices that we can live with, improving our lifeand society. The choices that need to be made in the modern biotechnological and genetic age aremany; they extend throughout life, from before conception to after death. The timing of reproduction,
contraception, marriage, are not new choices, but when we consider these issues it is clear that not
all people can exercise choice to the same degree, and that the limits to choice are determined byfamily, culture and laws which change over time. In order to inform prescriptive bioethics, we need todescribe the bioethics that people have followed in the past and the bioethics that they have today,
so that we can have a bioethics that better reflects what people in society actually desire (Macer,1994).

The theories of ethics
There are several basic theories of ethics. The simplest distinction that can be made is whether
they focus on consequences, actions or motives. Consequential arguments are the criteria
applied to assess the ethics of biotechnology applications, i.e. whether they contribute to thegreater good by improving the well-being of all. Consequential arguments state that the outcome can be used to judge whether an action was ethically correct or not. An action-based
argument looks at the morality of the act itself, so that the actual action to cause harm itself
is an unethical action regardless of the consequences or motives. Motive-based theories of
ethics, including virtue-based ethics, judge an action based on the motivation of the action.
For example, if the act was done with good intentions or not. Another separation that is used
is between deontological theories, which examine the concepts of rights and duties, and teleological ones, which are based on effects and consequences. If we use the image of walking
along the path of life, a teleologist tries to look where decisions lead, whereas a deontologist
follows a planned direction.
The objects and subjects of ethics can be viewed in terms of ecocentric, biocentric or anthropocentric concerns. Ecocentric concerns, that value the ecosystem as a whole, are used when
expressing environmental concerns. The reverence for all of life (Schweitzer, 1966) can apply
to the whole ecosystem or to every member of it. Biocentric thinking puts value on the individual organism, for example one tree or one animal. Anthropocentric thinking is focused on
the human individual. There is a trend for more ecocentric views to be included in legislation,with protection of ecosystems for their own value. While it can be useful to isolate distinct
issues, as will be done in this report, it is not realistic to separate human/nature and social
interactions. This is because almost all of human life is a social activity, involving many relationships with people and the ecosystem. Different ethics are implied when human activity, e.g.
agriculture or urbanization, attempts to dominate nature or to be in harmony with the environment. Given the international mandate of the World Health Organization (WHO), there needs
to be more work on world views of ecocentric and biocentric thinking if the WHO and United
Nations Development Programme (UNDP), and the guidelines for use of genetic engineering, areto represent the broad values of humankind.

Bioethics is built upon the long tradition of ethics, and it is actually difficult to draw a sharp distinction between the two, except to say that bioethics deals with the choices associated with the environment, biology and medicine. However, the ethical issues raised by biotechnology are commonly
termed bioethics dilemmas (Macer, 1990), although when we examine the actual moral questions they
may not be so novel and are often related to areas of applied ethics that were debated long before we
had modern biotechnology (Comstock, 2000).

Ethical, legal and social issues of genetically modified disease vectors in public health5People of all cultures have developed biotechnologies as they live together with many species in thewider biological and social community. A simple definition of biotechnology is the use of living organisms (or parts of them) to provide goods or services. Over five millennia of classical plant and animal
breeding have seen the emergence of agricultural societies, and modern biotechnology is built on that.
Efforts to find medicinal compounds in nature reach back even further into human history, while useof medicinal plants is observed in other primate species (Huffman, 2001). New technology has been acatalyst for our thinking about bioethics, and has been a stimulus for research into bioethics in thelast few decades.
Genetic engineering allows genes to be exchanged in a controlled manner between different species.
Since its invention in 1974, it has conjured up images of hope and dread. Public opinion is mixed, andis reviewed below. With the emergence of genomic sequencing, we now have the DNA sequence ofhuman beings, dozens of pathogens, and some disease vectors e.g. Anopheles gambiae (Holt et al.,
2002; Morel et al., 2002). It is therefore not surprising that molecular entomology, the study of DNA
and the proteins it encodes in insects, is emerging as a serious scientific approach for insect control,
as discussed in section 3.3 below.

1.3 Bioethics and molecular entomology
There is a long history of altering the behaviour of disease vectors so that they cannot transmit
pathogens to humans (Spielman and D’Antonio, 2001). Insects have also long been the targets ofattention in agriculture as well as in medicine. While there are few intrinsic ethical concerns aboutkilling insect pests, as discussed below, ecocentric approaches to ethics do raise some objections tomodification of ecosystem components, and these need to be taken more seriously (section 4 below).
TDR’s Steering Committee for Molecular Entomology has outlined a three-pronged effort towards developing genetically modified mosquitoes for malaria control. A similar approach can be envisaged in thenear future for other disease vectors, e.g. those of dengue and Chagas’ disease (TDR, 2002). First in
the process for each disease is to study host-parasite interaction; second is to develop methods totransform the vector; and third is to look at population ecology and genetics and at how to replace a
population of harmful vector insects with a population of non-harmful insects. This work has beendeveloping since a 1991 meeting on use of genetically modified (GM) mosquitoes to replace disease
vectors. Studies have shown that, among the 4000 known species of mosquitoes, only about 50 carry
human Plasmodium and only a quarter of these are good vectors for the parasite. In fact, most species
of mosquito are not anthropophilic (human-liking). It is predicted that, within several years, anAnopheles mosquito resistant to malaria may be made and that, by the end of the decade, the population genetics and ecology of these mosquitoes will be understood enough to use them for publichealth purposes to prevent malaria. The technology has been developing rapidly, and it has also been
predicted that similar approaches will be useful for preventing other diseases of high priority to TDR(TDR, 2002). In all these approaches, social factors need to be carefully considered (TDR, 2000[a]).
For the future, we can also imagine genetic modification of the pathogens themselves, and even of thehuman host, as methods for resistance to disease. The emphasis of this report is on the ethics of introducing GM vectors, especially insects, for disease control. The following section will review the history of modification of insect vectors, and section 4 will examine the ethical issues.

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Social, Economic and Behavioural Research • Special Topics No. 1

2 THE GENETIC ENGINEERING DEBATE
2.1 Growing use of genetic engineering
The licensing of the proteins insulin and human growth hormone made from genetically engineeredbacteria in 1982 signalled the beginning of the practical use of genetic engineering (Macer, 1990).
Several hundred biologicals have now been produced using genetic engineering and are in clinical use
around the world. Since the mid 1990s, foods produced from genetically modified organisms (GMOs)
have been sold in a growing number of countries (James C, 2001[a], 2001[b]).
There has been fierce international debate over the environmental and human health aspects of GM
foods, which has led to threatened trade wars between Europe and the USA. In general, no harmful
effects of GM foods on human health have been shown scientifically (FDA, 2001) and the careful choiceof non-toxic substances and avoidance of allergy-inducing proteins should mean that human health is
not affected. However, since it is always possible that someone will become allergic to GM food,labelling is important. There is greater concern over the environmental impact of gene transfer in theenvironment, which is discussed below. A wide range of information is available from all sides of thespectrum (FAO, 2001; CAC, 2002; USDOE, 2002).
A number of governments have considered the issues and concerns people have raised about genetic
engineering, and there is a wealth of useful material in the reports and submissions made to them(e.g. United Kingdom Royal Commission 1989; Catenhusen and Neumeister, 1990; New Zealand Royal
Commission, 2002). Some of the major issues are discussed in this report. Reports have also been madeby independent organizations on the ethical issues (e.g. Nuffield Council on Bioethics, 1999[a]).
Despite this widespread debate, the future of food production is tied to the use of varieties of plants
and animals made from genetic engineering, especially for developing countries that face food shortages (UNDP, 2001).
Some drought-stricken countries in Southern Africa, e.g. Zambia, have recently taken decisions toreject food aid because it may contain GM food, the same GM food which is common in North America
and some other major exporting countries e.g. Argentina. Some developing countries, however, accept
the use of GM food and crops. China was among the early developers of GM crops, while India accepted the use of GM cotton in 2002, after several years of internal political debate over whether genetic
technology was safe and whether the technology would make farmers dependent upon foreign imported seed and technology.

2.2 International systems for consideration of genetic engineering
A formal emphasis on ethical issues has only recently been considered in the UN system, despite major
work in academia and nongovernmental organizations (NGOs) in the 1980s (Macer, 1990). For example, the 1991 Food and Agricultural Organization (FAO)/World Health Organization (WHO) Conference
on Food Standards, Chemicals in Food and Food Trade recommended that the terms of reference for the
Joint FAO/WHO Expert Committee on Food Additives be expanded to include biotechnology, but that
this body should "only consider scientific issues regarding health, safety and technical concerns andshould not be involved with socioeconomic, ethical or like issues which properly should be addressedin other fora" (Macer, 1999). In the Codex Alimentarius Commission (CAC), the joint FAO-WHO
Intergovernmental Commission to set food standards (discussed in section 2.3), ethical issues areoften shuffled between committees for political purposes as there may not be international consensus. In fact, safety is based within the ethical principle of non-maleficence or "do no harm".
In over 100 interviews conducted by Macer (1999) on the ethical issues of food and agriculture at the
responsible UN agency, the Food and Agricultural Organization (FAO), the issues raised by GMOs andGM food were cited more than any other single ethical issue. Although a wide range of ethical issues

Ethical, legal and social issues of genetically modified disease vectors in public health7

exists in traditional practices and social systems, the new technology of genetic engineering is a catalyst for people to think about ethical issues. Member countries need guidance, including expert technical guidance, not only on the narrow issues and implications of selected genetic technology but also
on the broader issues that many are concerned about e.g. the intrinsic ethical issues of genetic engineering, intellectual property, and economic control of genetic resources and agriculture in general. In
2001, FAO released a brochure on the ethics of GMOs; United Nations agencies are, in general, supportive of the use of genetic engineering to help people in every country (UNDP, 2001).
Despite this largely technical work, there is still a need to look at the underlying ethical issues thatmake genetic modification a concern. The holistic definition of health as represented by the WHO definition of health as a "state of complete physical, mental, and social well-being and not only theabsence of disease and infirmity" is consistent with consideration of the broader social and ethicalissues as part of technology assessment.

2.3 Codex Alimentarius Commission and the safety of genetically modified food
The issue of food safety is not directly relevant to the question of use of GM disease vectors in public health, but because of its central importance in the public attitude to genetic engineering in general, international mechanisms for its regulation are of interest. Also to be considered is that, bygenetically engineering a trait in a crop plant, it might be possible to complement vector control, e.g.
by using plants to release insecticidal toxins that inhibit the breeding of vectors.
In 2000-2001, WHO (WHO, 2001) convened a series of expert consultations to address the safety offoods derived from biotechnology. The consultations addressed overall aspects of the safety assessmentof genetically modified foods of plant origin and of foods derived from genetically modified microorganisms (GMMs), as well as the potential allergenic effect of foods derived from biotechnology. There
were technical papers on the concept of substantial equivalence and GMO products, and other documents produced by the Organization for Economic Cooperation and Development (OECD) for the G8summit meeting in Okinawa in 2000. FAO (1997, 2001) has also released reports on the theme, andpromotes safe use of biotechnology.
Human health aspects of GM food are specifically reviewed by the Codex Alimentarius (CAC) Ad hoc
Intergovernmental Task Force on Foods Derived from Biotechnology (established by the 23rd CAC, June1999). This Task Force has developed model risk assessment guidelines for genetically modified foodsin general, as well as those from GM plants and microorganisms, as strategies for dealing with emerging food quality and safety issues (CAC, 2002), and has produced a number of reports e.g. Draft
Principles for the Risk Analysis of Foods Derived from Modern Biotechnology, Draft Guideline for the
Conduct of Food Safety Assessment of Foods Derived from Recombinant-DNA Plants, Proposed Draft
Guideline for the Conduct of Food Safety Assessment of Recombinant-DNA Microorganisms in Food. The
role of this Task Force in the management of ethical controversies is discussed in section 4 below.

8Social, Economic and Behavioural Research • Special Topics No. 1

3 MODIFICATION OF VECTORS AND PATHOGENSFOR PUBLIC HEALTH PURPOSES
3.1 Methods used for control of insect vectors
A biological method that has been shown to be effective in the field for area-wide control of some
insects is the sterile insect technique (SIT), which involves raising large numbers of insects that arethen sterilized by irradiation before being released in the field (Lachance, 1974). If sufficient numbersof competitive male insects are released, most wild female insects mate with these and thus produce
no viable offspring. There have been some successful programmes in different areas since the 1970s,
e.g. for eradication or suppression of Mediterranean fruit fly (Mansour and Franz, 1996), screw-worm
in Central America (Krafsur and Lindquist, 1996), tsetse fly from Zanzibar, melon fly from Okinawa, andmedfly from Mexico (Krafsur, 1998).
The SIT has been used mainly in agriculture. Although at the start of the 1970s entomologists began
to switch from reliance on chemical pesticides because of concerns over pollution and the problem ofresistance to pesticides, trials of SIT against rural mosquito populations in villages in India and Central
America have encountered problems due to immigration of females already inseminated with fertile
sperm. Other insect control measures that are more widely used include biological control, pheromonesand biological pesticides. In the case of human disease, physical barriers such as bednets are very
important (D'Alessandro et al., 1995). However, even in the year 2000, WHO argued that DDT was still
essential as a mosquito control agent, despite its withdrawal from agricultural production systems
because of health concerns. This meant that DDT for vector control was exempted from the Stockholm
Convention on Persistent Organic Pollutants agreed upon in December 2000 (UNEP, 2002).

3.2 Past attempts at introduction of modified pathogens
The drawback of the SIT is that it is not really applicable to mosquitoes over large areas because of
the numbers involved. A method that modifies insects in situ (in both urban and rural settings) isneeded to target a disease vector that is spread over a large area. This is where genetic engineering
may be useful.
Successful agricultural products based on modified pathogens were introduced more than a decade ago.
The World's first commercial pesticide based on a live genetically engineered organism was licensed forsale in Australia in March 1989 (Wright, 1989). It is Agrobacterium radiobacter var K1026 (Nogall), andit protects stone fruits, nuts and roses from crown gall disease. This "pesticide" consists of a harmlessstrain of the disease-causing bacterium that lives on the same leaves as the disease-causing strain andproduces an antibiotic which kills the latter. The gene for the antibiotic is placed on a plasmid which
has been engineered so it will not transfer to disease-causing bacteria and make them resistant to theantibiotic. There was an eighteen-month trial prior to the commercial release of Nogall.
In the year 2001, the first US field test of a genetically modified pink bollworm, a cotton pest, wasconducted. It followed very soon after the development of methods to transform the bollworm(Peloquin, et al. 2000), suggesting that some researchers may go to field trials within one to two yearsof transforming an insect species. About 3600 moths were studied in a field enclosure of more than
one hectare, after being modified with green fluorescent protein (GFP) as a tracing gene. This was
based on the idea that a lethal gene can be introduced to kill the progeny of both engineered moths
and moths which breed with them (Dalton, 2001). In the short term however, the presence of GFPmeans that the sterile insects can be readily distinguished in the field. This itself is a significant
advantage because currently farmers may have to release up to 60 times the number of sterile insects
in the field to control bollworm, but these numbers might be brought down twelve-fold if the sterile
insects can be easily identified in the field.

Ethical, legal and social issues of genetically modified disease vectors in public health9A further area is to attempt biological control of disease vectors by introducing specific pathogens(Lacey and Kaya, 2000). Genetically modified pathogens will be more selective and/or more efficient
at killing their vectors. These concerns have to be considered in view of alternative methods of killinginsect vectors, such as with pesticide impregnated physical structures that fool insects into attacking
them (News report in Science, 2001). The species specificity of such structures is one ecological concern; however, they may be more predictable than purely ecological methods. One strategy would beto genetically modify the vectors so that they are attracted to bait or physical structures as a method
to kill them.

3.3 Development of genetic modification techniques
While there is debate over the use of funds to combat infectious disease using genomics and biotechnology as opposed to implementing practical measures to curb vectors and pathogens in the field (Curtis,
2000), it is widely agreed that the former approach will be a major strategy in the future (Hoffman, 2000;
James et al., 2001). Genetic engineering can be defined as the modification of genetic material by recombinant DNA techniques, including the deletion, modification, or insertion of genetic material in agenome. For example, a new gene may be added to a genome to add a new function or make a new
enzyme. This gene can be from any species, as all organisms use the same DNA coding system.
A common way to insert DNA for genetic transformation of insects is to use transposons or viruses(O'Brochta and Atkinson, 1998; Lewis et al., 1999). Naturally occurring arboviruses (which do notinfect vertebrates) can be modified to express and silence genes in mosquitoes so that determinantsof vector-pathogen interactions and other important vector phenotypes can be characterized rapidly(Olsen et al., 1996; Johnson et al., 1999), while mosquito-specific viruses could also be engineered toincrease or enhance their biopesticide capability (Ward et al., 2002). Other systems of gene modification may also be developed, given the large amount of attention to genetic engineering in medicine
and agriculture. Most attention has been given to efforts to genetically transform insects in the laboratory, and to test their behaviour before releasing them into the environment. A mechanism thatwould safely spread the gene among vectors in the wild is the objective of these studies. There is asignificantly hurdle to engineer genes into mosquitos in the wild even if this can be accomplished in
the laboratory. There is still a major problem about how to effectively drive genes into vast wild populations after they have been engineered into a few laboratory mosquitos.
Transposons, also known transposable elements, facilitate their own excision and re-integration intoanother site in the genome using enzymes called transposases. Transposons can be constructed toinclude any gene combination, and they are microinjected into insect embryos for integration into
DNA. Transposable elements can contribute to genome evolution in nature, but the way they invade
the genome and are regulated remains one of the major questions in population genetics (Ladeveze etal., 2001). Over the past twenty years there has been much research on transposable elements in
Drosophila (the fruit fly), while a dozen other insect species have been genetically modified by usingtransposons (Atkinson et al., 2001).
In order to easily identify and select genetically transformed insects from those not transformed, amarker is used. A universal marker that is used to follow gene transfer in any species is GFP from thejellyfish. So far, GFP has been used in flies, mosquitoes and beetles (Berghammer, 1999). Enhanced
green fluorescent protein (EGFP) has been transferred to Anopheles stephensi mosquitoes (Catteruccia
et al., 2000). Use of luciferase as marker gene has also been reported (Johnson et al., 1999).
To genetically manipulate disease vectors, transgenesis systems must be developed (Alphey et al.,2002). Effector molecules must be identified that will induce the anti-pathogen phenotype in the vector, and mechanisms are needed to drive the effector system into the vector population (Beaty, 2000).
The latter step raises more ethical issues about the safety and desirability of changing the entire vector population, and possibly related species, as will be discussed in the following section.

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Apparently stable, germ-line transformation has been achieved in mosquito species that transmit yellow fever, dengue, LaCrosse encephalitis and malaria, using varying techniques and DNA delivery vectors (McCullough, 2001; Lycett and Kafatos, 2002). However, the efficiency of genetic transformationneeds to be improved from the under 10% at present. Researchers have genetically manipulated a mosquito, using a Sindbis virus expression system, to express antibodies against the malaria parasite, andthis has reduced the number of parasites in the insect’s salivary glands by 99.9% (Capurro et al, 2000).
Ito et al (2002) reported that they had generated a strain of Anopheles stephensi mosquitoes which
carries a piece of DNA that induces the production of a peptide (SM1) after blood feeding which blocks
transmission of the parasite. These are important steps towards the genetic modification of mosquitoesthat transmit malaria to humans (Enserink, 2000).
Some efforts are focused on genes that enhance insect immunity to pathogens. In Aedes mosquitoes,
expression of a gene has been controlled with a regulatory sequence (promoter) that is activated by ablood meal, since disease agents are spread in mosquitoes after the ingestion of infected blood(Moreira et al. 2000). Aedes aegypti is important for transmission of dengue fever in Latin America andelsewhere. Resistance to dengue virus transmission was one of the first reports of genetic modificationof mosquitoes (Olsen et al., 1996).
Genetic transformation in Anopheles has not progressed as far as in Aedes because of the greater difficulty of manipulating the more fragile Anopheles embryos. In 2000, scientists working in the UK,
Germany, Greece and Brazil achieved transformation of Anopheles gambiae cells, the major malaria vector in Africa, and Anopheles stephensi embryos, the major malaria vector on the Indian subcontinent(Catteruccia et al., 2000). Once the hurdle of genetic transformation is overcome, almost any target
gene can be modified and many approaches can be attempted.
Another potentially useful system is a so-called "terminator" gene, constructed in the Drosophila system (Heinrich and Scott, 2000). This gene is, under certain conditions, lethal to transgenic females but
otherwise has no effect on either male or female viability. It is considered especially useful for sterile
insect release programmes, when only males are released because transgenic females would be killed.
An alternative approach to transforming insects for disease control is to transform the bacterial symbionts living within the insect (Beard et al., 1998). This paratransgenesis approach has been utilized
to explore means of preventing transmission of American trypanosomiasis (Chagas disease) from triatomine bugs and of African trypanosomiasis (African sleeping sickness) from the tsetse fly. Bacteriathat populate the guts of these insects, necessary for parasite development, may be altered to prevent
pathogen development. A potential method for field dispersal of GM bacteria to control Chagas diseasetransmission has been developed and is being prepared for field testing.

3.4 Modification of other organisms
One option is that a plant, such as corn, could be genetically modified to express specific insecticidal
toxins from Bacillus thuringiensis (Bt). These toxins would be carried by the wind into mosquito breeding areas as a larvicide (Spielman and D’Antonio, 2001). In the past, large areas were sprayed with Bt
spores, but now Bt is widely used in genetically modified corn and other agricultural crops. Microbial
control agents like Bt have been established as a commercially viable and promising alternative to conventional pesticides. They have high efficacy and good environmental safety (TDR, 2002). However,resistance to Bt has developed in several target species, and given its importance in agriculture, studyof the ecological implications of this type of approach may prove to be equally complex as direct modification of the vectors themselves.
The use of beneficial organisms for the control of mosquitoes was first recognized in the 19th century, when attempts were made to introduce predators such as dragonflies (Lamborn, 1890). However,mass breeding and successful introduction of predators such as hydra, flatworms, predacious insects orcrustaceans, often bring a range of problems. Such problems did not occur, or to only a limited extent,with the use of fish such as the mosquito-eating fish Gambusia affinis, which was successfully intro-

Ethical, legal and social issues of genetically modified disease vectors in public health11duced into many countries to control mosquito larvae in the early 1900s (Legner, 1995). Telapia fish
have been introduced in the Phillipines. In these cases, people eat the fish, so there is potential benefit in introducing them in integrated pest management of agricultural production systems. However,concerns have been expressed because some of the fish may eat other species of animals (Laird, 1997;
Service, 1995), and this may be one reason for the declines seen in amphibian populations.
A further possibility would be to release a transgenic predator of the vector species, e.g. geneticallymodified mosquito-eating fish. Use of modified animal hosts in agriculture will provide some lessons
on the ecological success of such an approach. For example, research has been carried out to makesheep resistant to blowfly strike, which causes the deaths of many domestic animals in New Zealandand Australia (Scott, 2001). Genetically modified blowflies have been made, and an objective of theresearch is to develop a system that is lethal to the flies under certain conditions only. The immediate plan is to develop the project through use of the sterile insect technique, but the future target is
to modify the sheep. In Australia, genetic modification of European carp, an invasive species of fish,
is being tested to eliminate the pest through daughterless gene transformation (Nowak, 2002).

3.5 Modification of human hosts
In vaccination, modified pathogens may be used as immunogens to develop resistance to the pathogen
in the host. We can also consider developing genetic vaccines by modifying the host rather than thepathogen. Immunization has few ethical dilemmas unless there are substantial risks to those being
vaccinated. Human genetic modification has been the subject of extensive ethical reflection (Macer,1990), and the general consensus is that vaccination of individuals is ethically justified when proveneffective and safe. However, because pathogens often mutate rapidly to develop resistance to immune
defences, the prospect of making vaccines for all human diseases cannot be relied on.

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4 ETHICAL AND SOCIAL ISSUES
4.1 Reducing the complexity of ethical and social issues
One of the reasons why there is such confusion over the use of genetic technology is the failure to deal
with ethical and social issues in an organized manner. Perceived complexity has also been a barrier toprogress in regulations covering the environmental release of genetically modified organisms. While
natural science attempts to tackle problems in a systematic manner by controlling variable factors toallow experiments, ethical dilemmas are not normally dealt with in a systematic framework. Often scientists consider that ethical issues are too complex to discuss rationally, and debate on genetic engineering is spoilt by the failure to clearly identify specific moral questions that need to be answered.
After looking at what type of framework might be needed, the section below considers a number ofsocial and ethical issues.
As discussed in the introduction, there are several ways to approach bioethics: descriptive, interactive
and prescriptive. These aspects are included in the TDR mandate. Each aspect is related to the other,
but a collective approach needs to be thorough. The United Nations Development Programme (UNDP)
and WHO have duties to all member countries, which they fulfil by gathering and sharing information,a descriptive function. A further function, that may extend to being prescriptive at times, is as a centre of excellence at international level. WHO and UNDP are intergovernmental forums that have aunique role for interactive ethics. They also host a variety of informal technical meetings, receive
expert consultations, support symposia, and provide expert technical advice on policy, some of which
relates to ethical choice between different technologies based on concerns beyond raw productivity. A
unique role at intergovernmental level for WHO and UNDP is to consider how ethical values can beincorporated into policy beyond the economic efficiency arguments that may be more dominant in theeconomic analyses discussed by national governments, when trade principles have highest priority.
These complex issues need to be dissected so that they can be easily identified and incorporated inpolicy, planning and action. The ethical, legal and social issues identified have been separated into
several categories, as outlined below.
(a) Opportunities and problems that can be addressed on an ongoing basis if appropriate
mechanisms are introduced and maintainedSome issues may be resolved to the extent that they do not need ongoing management or review, andmonitoring mechanisms can minimize any conflicts between parties and safety problems. All partnersof TDR, UNDP, World Bank and WHO may need, however, to develop new infrastructures and training todeal with component ethical issues as they arise in rapidly emerging areas such as biotechnology. This
will help supplement the legal guidelines that have been produced in some member countries to coversome applications of biotechnology, and international undertakings such as the Cartegena Protocol on
Transboundary Movement of Live GMOs (CBD, 2000).
Specific opportunities can be identified and incorporated in policy, planning and action. TDR is called
on to take a stand (for, against, or no comment) on issues that relate to WHO's unique international
mandate to promote health for all, including for the future of humankind, and to be involved in areas
where it can be a productive partner in achieving the general goals for member countries. Taking astand may e.g. be to say that a given technology is good or bad in a given case; it could also mean
taking a decision not to comment.
One of the main concerns of releasing GMOs is environmental risk (FAO, 2001). This risk has been successfully controlled in over 10 000 international field trials of GMOs (USDA, 2002). Whilst the methods
used for monitoring field trials are argued to be inadequate by those campaigning against GMOs (Ho,
1998), to date there has not been a significant adverse event from GMO release for the health of any
non-target organism, including humans, in the ecosystem (Comstock, 2000). There are concerns over

Ethical, legal and social issues of genetically modified disease vectors in public health13

unknown long-term effects, which could call for ongoing monitoring of farming systems. In fact, thelong-term effects of using living organisms in general, not just GMOs, is a useful area of study that
could potentially benefit humankind and ecosystems much more than the use of one particular GMOs.
Although the environmental release of GM insects may require an extra level of containment, the system falls within this first group of ethical issues because any specific issues can be dealt with in a systematic manner by designed experiments.
(b) Controversial issues considered at global level but not resolved
The GM food debate has been an extremely controversial area of science and technology, and is one
that WHO and FAO have constitutional mandates and moral obligations to consider under the themesof food security and food safety of the Codex Alimentarius Commission (CAC, 2002). The Codex
Alimentarius Ad hoc Intergovernmental Task Force on Foods Derived from Biotechnology (establishedby the 23rd CAC, July 1999) is currently developing a strategy for dealing with emerging food quality
and safety issues (CAC, 2002). It is hoped these issues will soon move into group (a), if an international mechanism can be agreed upon. However, other legitimate issues for the GM debate, e.g. ethical and social concerns beyond food safety and traceability, are likely to remain in this group of unresolved issues for some time.
The international movement of live GMOs between member countries is covered by the Cartegena
Protocol on Biosafety under the Convention on Biological Diversity (CBD, 2000). This convention created a set of biosafety clearing houses in member countries that identify responsible persons in eachcountry, as will be discussed below (regulatory section). However, the issue of environmental releaseof GMOs, and trade in food produced from GMOs (not live GMOs), continues to be vigorously discussed
and remains a public controversy.
While the food crisis is well documented, when UNDP (2001), in its Human Development Report, supported the use of GMOs to provide food to developing countries, there were many opposing voices.
Opinion surveys in which people have voiced more fears about insects and animals than plants, allow
us to predict that any release of insects will be controversial. Public concerns have already been
expressed about agricultural trials of GM insects (Union of Concerned Scientists, 2001).
The approach to resolving these issues will be to move components of controversial issues into manageable areas (of type [a]). Specific controversial issues as given above should be defined scientifically, analytically and rationally, and discussed with the intention of shifting them into manageable
areas within the framework rather than being broadly debated, when they often don’t get resolved.
(c) Controversial issues not considered
New ethical issues about GM arthropod vectors and their symbionts and/or pathogens should be subject to extensive open discussions and forums. Some of the new technical possibilities need to beassessed for the ethical and social issues they raise. TDR policy decisions are required as to whether
TDR should play a major role in any of these areas, but especially in the field of opening up new areasfor public discussion. Should the role be to stimulate other organizations to take effective action towork towards resolution of emerging issues? TDR is called to respond to all issues within its mandate
and, even if it is not to reach a stand on a particular issue, TDR does have a role to play as the mostappropriate intergovernmental forum for discussion of these issues.
As discussed below, practical guidance for ethics committees needs to be clarified on public healthinterventions. One key problem is identifying who is specifically at risk, and what the particular risk
is. In vector release studies, everyone in the area may be at risk. These complex questions are mademore manageable through breaking down the concerns people have into manageable areas. Defining aminimum standard of protection for research participants in trial and control populations for GMO interventions is the key point. This issue is not specific to GM vectors and pathogens, but it is crucial toconsider the benefit/risk equation.

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4.2 Intrinsic ethical issues of genetic engineering
The conclusions of studies of ethical issues inherent to the process of genetic engineering compared
to traditional methods of animal and plant breeding, are that the only significant differences in theprocess are the more precise control of genetic engineering and whether the DNA involves cross-species
gene transfer that does not occur in nature (Macer, 1990; Nuffield Council on Bioethics, 1999[a];Comstock, 2000).
One of the key questions is whether there is an intrinsic value of genetic integrity at an organism andecosystem level which humans should not change. One way to consider this question is to note thatcross-species DNA transfer does occur in nature between all species, even of different kingdoms, andthat the genomes of insects are subject to genetic flux in nature (Macer, 1990). In this sense, because
the DNA change can be precisely designed, an actual targeted genetic change through genetic engineering should be safer than a natural change because it is more under control. Given the results ofpublic opinion surveys that find opposition to cross species gene transfer (Macer, 1994; Macer and Ng,2000), if the DNA change is made using DNA within the same species entirely, then this concern can beremoved. Therefore, there is no new intrinsic ethical dilemma from the modification of DNA structure in
genetic engineering as it simply mimics the natural ways organisms use to change genetic structure.
One argument used in these discussions concerns the telos (purpose) of an organism. A teleologicalexplanation describes phenomena by their design, purpose, or final cause. Teleology is the branch ofmoral philosophy dealing with the cause and effect of an action, the belief that there is purpose anddesign in nature, and consequently, with the belief in the existence of a Creator. There are concernsthat the ability to alter the telos of an animal has profound implications (Munro, 2001). If one believes
that every organism has a purpose, then the telos is an intrinsic concern, and genetic engineering
alters the telos or "being-ness" of an organism. However, it is debatable whether changes and control
through genetic engineering are significantly different from changes made by humans to animals andplants in farming and modern life. It is basically an issue of human control of nature, and there isdebate over the extent to which humans should control nature (Macer, 1990; Comstock, 2000). If weconsider this issue in a historical context, we see that humans in many affluent cultures have controlled nature in significant ways, e.g. by concrete river banks, irrigation and sanitation projects.
However, especially in some developing countries, limited resources have meant that control of nature
has been less.

4.3 Animal rights concerns
Another concern in ethics when discussing animals is their capacity to suffer or feel pain. If insects
do not feel pain or sense feelings, then the most prevalent ethical approach for animals would argue
that there is nothing intrinsically wrong in manipulating them (Singer, 1976). However, if we consider the idea of making so-called vegemals, animals that do not feel pain, we are still manipulating life
for human purposes without considering the interests of the animal (Macer, 1989). The concern is thatliving organisms should not merely be treated as a means to the ends desired by humans.
Animal rights concerns about the genetic modification of higher animals, e.g. mammals or birds, mean
there is more ethical concern about modifying sentient animals, and more public concern, than if insect
vectors were engineered. In addition to so-called intrinsic concerns (pain, sentience, consciousness),
there are also extrinsic values placed on some animals by human society. For example, some animals
are national symbols and people have greater concern about harming them. There are also biodiversity concerns about endangered animals, some of which are expressed in the Convention on Biological
Diversity.
While perhaps only followers of the Jain religion in India regularly refrain from killing insects that arehuman pests, there are still some people who may object to killing mosquitoes. It is not known if
manipulating the insects so that they would not be a human pest would be more acceptable to per-Ethical, legal and social issues of genetically modified disease vectors in public health15

sons with these ecocentric world views than traditional methods of insect control that attempt to eradicate a whole insect population.
Those who subscribe to an ecocentric viewpoint might argue that the ecosystem as a whole would benefit from an intervention that left the mosquitoes in the ecological community, with the eliminationof the disease-causing pathogen from the vector, if the alternative was eradication of the vector
species. In this case the total number of species affected by this type of genetic modification of vectors would be less than the species affected by the use of pesticides. However, there are still those
who believe there should be no human modification of the ecosystem. This actually should argue that
there should be no direct or planned modification of an ecosystem by humans, since human activity
modifies almost all ecosystems, including those where humans are not directly a component member.

4.4 Consent from trial participants
Recognition of the ethical principle of autonomy means that all participants need to give informed
consent to an intervention that has a reasonable risk of causing harm (Annas, 1989). There are significant difficulties in obtaining individual informed consent in some developing countries (Ekunwe
and Kessel, 1984; Angell, 2000; Alvarez-Castillo, 2002), but by adequate investment of time and provision of suitable materials, it should be possible to obtain informed consent from individuals at direct
risk, even though the exact cultural interpretation of the informed consent process may vary between
countries (Nuffield Council on Bioethics, 1999b; Alvarez-Castillo, 2001). There are risks of direct orindirect harm to human beings from the original pathogen-transmitting vector, so a trial needs to bedone to show that there is greatly reduced risk of harm from the modified vector. This is the whole
purpose of the project to create modified vectors, to reduce risks. Until a trial is made we cannot be
sure that there will be no risk and that the whole enterprise has been successful.
The risks may not just be those that arise directly from the ability of the vector to carry the target
pathogen. There could be a negative impact on human health by altering the behaviour of blood-feeding insects. In the case of insects that cannot be confined to a particular population, whether theyflyor float to new places, notions of "human subject" and "informed consent" need to be extended.
There are basic ethical issues involved in vector collection and studies in the field. Firstly, many suchstudies have relied on a researcher waiting for the vector to land on a human host, and then capturing it hopefully before the vector has transmitted the pathogen to the "bait". In fact, any field studies in which human beings are exposed to the pathogens raise the question as to why some other intervention is not used in that area.
The approach developed for population genetics studies may be useful where the community and local
authorities are involved in the decision-making process. Informed consent requires information to beprovided, so disseminating information about the plans and progress of the project, and obtaining theconsent of any person potentially affected by the release of transgenic insects, is important for the
ethical conduct of research trials, whether or not national guidelines require this, or even exist. Other
lessons show us that people who lack the means to express their preferences may have been abusedby the lack of individual or community consent for research in anthropology (Fine, 1993; American
Anthropological Association 1998; Kleinman, 1999) and epidemiology (Capron, 1991; Dickens, 1991;
Gostin, 1991; Chee et al., 1996). Recent examples include the collection of genetic samples from persons in China by US researchers without any informed consent, or with forged informed consent documents. This shows us that even institutions that claim to have high ethical standards abuse research
participants (Lawler, 2002).
If a study involves humans, oversight by an ethics committee or institutional review board (IRB) is
necessary. In an increasing number of countries, such committees are established by law and arecharged with certain legal responsibilities, typically about the conduct of research or clinical practiceat local or national level. An IRB is a group of persons from a range of disciplines who meet to discuss the ethical issues of particular submitted procedures and review the benefits, risks and scientific

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merit of the application. The IRB usually requires that each human subject in a medical trial gives
informed consent to be involved in the project. Model ethical guidelines on the establishment and procedures for an IRB have been produced by an international consultative committee for TDR (WHO,
2000). These guidelines however are not sufficient for the broad question of how to obtain informed
consent for a public health intervention involving thousands of persons where the benefits are notdemonstrated.
Ethics or bioethics committees include groups of people set up to adjudicate about bioethical matters.
An IRB is in a sense an institutional ethics committee, but a typical IRB works through a large number of applications and often excludes the broader social discussion and representation that is seen ina regional or national bioethics committee. There are also national variations in the laws to define
membership and scope of work, and terms used. The project to introduce transgenic insects will needan ethics committee with a broad overview, and specific regional ethics committees to consider the
local issues.
To consider the issue at a local level, as required for obtaining appropriate informed consent, it isessential that a local ethics committee (and/or IRB if associated with an institution) open to the communities involved is established. There are cultural differences in the way informed consent should betaken (Levine, 2001; Alvarez-Castillo, 2002). The accepted norm in international ethical guidelines isseen for example in the modified Helsinki Declaration and the draft Council for International
Organizations of Medical Sciences (CIOMS, 2001) guidelines. In cases involving bilateral research collaboration, the most stringent ethical standards of the two countries should be applied. This createsproblems for non-literate populations, and for populations whose common sense social assumptions aredifferent. It is desirable that internationally agreed standards are applied, and that there are few points
of difference between these standards even for simple clinical trials of drugs. The ultimate decision
procedure should be decided by the local ethics committee, but international consistency and guidance
will be essential.
Although the control population for the study may continue to face the same high risk of contracting
the disease, recent trends in research ethics debate whether we can leave control groups without any
treatment. Therefore, ethically there may need to be some other vector reduction measures given if
making any interventional study in an area. While those designing ethical guidelines on placebo-controlled trials (e.g. Helsinki Declaration) were thinking of placebo controls on clinical trials of potential
medical drugs, we can ask the ethical question whether researchers have an obligation to the local population to use the best available means of disease control whenever they enter an area for a study. This
practically means that, as well as studying the new method, a researcher may ethically be compelled
to also provide the best available proven alternative to the study population. There may be times when
the provision of the proven alternative to the area of study alters the dynamics of the disease so thatthe results of the vector field trial differ from what the results would have been had no establishedalternative been provided.
Before and during the intervention, there may be privacy concerns when questionnaires are administered and personal data are stored. For public health purposes, it is essential that all information about
individuals involved is linked to other data, but to ensure privacy, the data should only be identifiable
to a specific person by a coding frame that is not in a computer linked to a network.
One of the ethical traditions in TDR is the effort to free children from the burden of often forgotten
tropical infectious diseases. Children are therefore one of the targets of public health interventions,with presumed consent from the therapeutic imperative that they want to be involved in programmesthat will avoid disease. Some compulsory vaccination programmes have faced criticism that consent is
not obtained even from the surrogate decision-maker, the child's parents. In each family there may several adults, and more children, which raises questions of whether consent is required from every individual. The local cultural norms need also to be considered. However, an appropriate mechanism may
be one in which the views of everyone of reproductive age (let us call this the level of adult maturity)
are gathered, and consent sought from these persons both as individuals and as a family.

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The agreement and understanding of children in the community should be sought through suitablematerials. However, children should not be exposed to direct risk from therapeutic trials unless there
is no alternative. In the case of a child living in a community that was involved in a GM vector trial,
no direct risks to the human population would be expected so the consent issue is not a major hurdle.
On a more positive note, children in fact could be a very powerful means to involve the community ina process of community engagement through schools. Since children are at higher risk from many ofthe diseases in question, they stand to benefit more, and most parents may want to be involved in thetrial because of the potential benefit to their children rather than themselves.

4.5 Consent from broader society on environmental risks
The human community also needs to consent to the environmental risks of a trial as these represent
potential harm to other members of the biological community as well as other members of the humancommunity. Globally people vary in the importance they ascribe to the environment, or parts of it.
Especially in areas where more traditional world views are found, we may see greater value given toparts of the environment that are forgotten in the modern industrial mindset. We also see variations
between persons in all cultures as to their images of nature and what is life (Macer, 1994).
Some people are willing to sacrifice themselves for the environment. Examples such as the preservation of sacred groves in India for thousands of years, even during times of severe crisis and humandeath (Gupta and Guha, 2002), show that in some cultures almost all people are willing to die rather
than damage that part of the environment they cherish. This behaviour is often linked to religious
beliefs in the afterlife.
A variety of potential broader ecological, environmental and health risks are associated with therelease of GM organisms. Environmental risks can be considered from both anthropocentric and ecocentric-based approaches. The risks identified include the possibility of horizontal transfer of the transgene to non-target organisms, and possible disturbance of insect ecology (Tiedje et al. 1989; Hoy,
1995; Nuffield Council of Bioethics, 1999[a]). There have also been concerns expressed in some cultures, e.g. New Zealand, over the need to value the native fauna and flora, which is considered by manyin the Maori community to be something not to modify (New Zealand Royal Commission, 2002). While
human beings cannot consent for other organisms to be modified, very few persons suggest that any
consent is required except for possibly sentient animals.
Any risks to the agricultural systems of rural communities also require assessment, as animal diseases
transmitted by vectors are important to farming families. In addition, there may also be risks to wild
animals in surrounding areas, which in some ecocentric environmental views have more intrinsic rights
to be left undisturbed than farm animals (Rolston 1994). This calls for broad ecological understandingof the impact, beyond public health.

4.6 Social consensus building and early cessation of trials
If the trial covers an area with a local population of 100 000 persons or more, it is unrealistic andunlikely that informed consent can be given by all people in the area. There will always be some people who are against any proposition, no matter how much others value it, but the opponents cannot
be moved from their houses for the period of the trial. So a procedure that is neither paternalistic nor
paralytic needs to be developed. How can we resolve the conflict between not being paternalistic(which means asking all citizens for their consent) and the impracticality of waiting for every single
person in a community to agree?
After the process of consultation and dialogue to seek informed consent, there still needs to be a procedure to supply relevant information to all persons in the area so that the minority who disagree withthe trial have the option to leave. In developing countries, many may not realistically be in a position
to achieve social consensus. The mechanisms for social consensus in biotechnology are not well under-

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stood in the affluent countries who have been debating GMOs, and even less is known in developing
countries. Public opinion studies suggest that people may respond differently to theoretical and realsituations. There is therefore a need for further research in parallel to the trials, so that realistic cases
can be faced by a community and the process followed.
It is possible to imagine the rumours that would arise mid-trial should an uncontrollable event likeflooding ever result in an increase of vector population. The increase in vector population might befalsely attributed to the trial of the modified vector and result in increased public opposition to thetrial. Perhaps, in such an event, community opposition to the trial grows from 5% to 30%. Can consent be withdrawn at this stage, as it can by an individuals who are participating in a clinical drug
trial? Release of a modified vector is not the same as a clinical trial of a new drug. Once started, allpersons in the area have to continue to be subjects, until the point when the whole community decides
to stop the trial and the trial is terminated.
On the other hand, a contingency plan for an unexpected adverse event must be ready in case the modified vectors need to be killed. This could either involve a pesticide or a specific chemical designed to
selectively kill the modified vectors. It might be possible to insert specific chemical sensitivity in thevector by genetic modification. In this case, the added expense in terms of finance and risks to the environment and health for control of genetically modified vector trials having bad effects would be justified, whereas in the existing situation such control measures would neither be feasible nor appropriate.
After completing the field trial, if the modified vector is recommended for larger scale or general use,
it may not be practical to obtain the consent of everyone in the community. A referendum might be
the most appropriate method of providing information to the community, but there may be no way toaccommodate the wishes of a significant minority if the substantial majority agrees and the scientificevidence supports the intervention. In many endemic areas there are no appropriate political structures
to consider a referendum.

4.7 Equality and inequality in access
The rejection of interventions to reduce infectious disease by some members of a society, whether they
are national regulatory authorities or isolated local community leaders, will create inequality of accessto prevention, therapy and information. Although information should be accessible on the Internet,
and in many communities there will be someone who is able to access that information, there will be
a number of persons who do not have access or who are not sufficiently literate.
Regarding the actual intervention, a modified vector would likely be introduced in a whole community based on geographical or geo-biological features, regardless of wealth. In this way, the equity concerns may be less than with procedures such as localized pesticide dispersal or insecticide-treated bednets, which may be more common in richer areas than poorer ones. However, in case of any geographical clustering, this should not be preferential to the interests of the rich.
Any initial trial may be subject to the philosophy "not in my backyard". Socially powerful persons are
generally more effective at preventing trials they perceive to be risky in their area, or, conversely, atattracting social resources towards themselves and away from weaker persons in the community. It is
important that risks and benefits are shared equally, and one way to ensure this would be a commitment to the local community that, if the trial is successful, the full-scale intervention would include
them from the beginning. In this way, any risks borne by a local population would subsequently be
rewarded by that population being the first group to benefit from the knowledge gained when the fullscale safe and effective control programme is implemented. The field trial must therefore come with acommitment to the local community that financial resources will be available and that sustainable useof the control tool will be affordable.
Although there is no guarantee that a trial will succeed, the participants should still receive benefits
from being involved. These benefits include increased education about the disease and about the vec-

Ethical, legal and social issues of genetically modified disease vectors in public health19

tor’s role in transmission, which is essential for the process of informed consent. There should also be
immediate benefits from any disease prevention methods offered, e.g. access to pesticide treated bednets. The concept of benefit sharing is important and related to compensatory justice, as well as torecognition of the persons themselves.

4.8 Ethics of technology choices and knowledge development
This issue is linked to modernization. Issues include the ethics behind research into, and later financing of, technological products that attempt to "fix" a problem rather than invest in increasing the ecological knowledge base to "prevent" the problem. There is considerable preference for deterministic science over "softer" educational systems like flexible learning. Who’s science is "good" science?
Commonly there is a concept that broader impact issues should be dealt with after a technology has
been developed, sometimes called "externalities", rather than concentrating on precautionary and preventive actions. This issue needs long-term vision, which may relate to the short time frame for mostpolitical decisions as opposed to the long time frame for social and environmental improvement. The
ethics of calculating market costs versus ethical concerns about different options need to be considered part of the choice of technology.
It is clear that not all local communities will share the modern scientific world view that technicalhealing is better for them, so there needs to be flexibility in the approaches available to eradicate disease. In the past, paternalistic interventions were taken on the behalf of citizens; however, civil rights
movements have empowered people to take these decisions themselves. This general social background
could be considered the underlying basis for establishing the Steering Committee, in TDR, on Strategic
Social, Economic and Behavioural (SEB) Research, which states "a better understanding of how social,
behavioural, political, economic and health system factors operate to affect disease patterns and disease control methods will be important for identifying future needs, opportunities and innovations for
improved control of TDR diseases" (TDR 2000[b]). There is a clear scientific rationale for developing
these studies, consistent with the ethical principle of beneficence.
Any professional global organization in the 21st century is expected to give independent, balanced andprofessional technical advice that is suitable for local conditions. There are still questions to beresolved, such as "When should a professional body or expert offer alternative options beyond a list of
two initial choices that the country requested help to choose between, when the options are equally
viable and may reflect more the overall ethical mandate of TDR and/or the ethical culture of the member country?" When considering the ethical issues in preparing investment projects that TDR is called
upon for advice, lessons can be learnt from the environmental and social impact guidelines of bodies
like the Cartegena Protocol on Biosafety (CBD), FAO, and the World Bank. From these, more formal
guidelines for assessment of TDR projects can be developed.

4.9 Intellectual property rights and technology transfer
A number of ethical issues have been raised in international debates over the morality of patents(Macer, 2001), and there have been strong calls against the patenting of medical innovations (Nelkin
and Andrews, 1998). Laws on intellectual property vary between countries, despite attempts to harmonize these laws among industrialized countries and members of the World Trade Organization (WTO).
A number of developing countries are not members of the WTO, and often the major controversies overwhether a country will join WTO is related to intellectual property rights (IPR). In light of intellectual property considerations, some might ask whether it is ethical for TDR to provide a pathogen or vector to a local community for reproduction, knowing that the community will reproduce the agent without paying any royalties. The answer appears to be an unequivocal "yes", with the recent international consensus over interpretation of article 31b of the Trade-Related Intellectual Property Rights (TRIPS,
1994) agreement to give priority to medical emergencies. Article 31(b) says that something underintellectual property protection can be used without authorization of the IPR holder where the law of20

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a member state allows for other use of the subject matter of a patent without the authorization of the
IPR holder "in the case of a national emergency or other circumstances of extreme urgency or in casesof public non-commercial use". Thus, even the WTO has accepted that the global economic system issecondary to the ethical mandate to save human lives.
Utilization of genetic diversity as a tool of genetic engineering, and market commodification of genetic
resources, are concerns that have been widely debated by FAO and the Intergovernmental Commission
on Genetic Resources in Food and Agriculture (CGRFA) (IPGRI, 1997; Macer, 1999). The principle ofcompensatory justice would say that, since rich countries have benefited so much in past centuries
from the use of genetic diversity in poorer countries, the whole concept of recognizing only new genetic
variation in intellectual property is ethically questionable.
For a fruitful partnership in developing a technology, there is a need to seek the understanding andsupport of the holder of the IPR. The original maker may have the best expertise to continue improving the technology based on field experience, so should ideally be a partner in the planning of any
field introductions. Therefore, advance negotiation should be held with all patent holders, as in negotiations for the use of vitamin A-enhanced genetically engineered rice in 2000-2001, so that they support use of the technology to save lives in developing countries. With sufficient public pressure, which
can be ethically pushed by WHO for the purpose of saving life, and an ethically conscious industrial
community, the IPR problem should not be a barrier to any GM medical project in the developing world.

4.10 Inducement to participantsWith regard to human genetics sampling, the Human Genome Organization (HUGO) Ethics Committee(1996, 2000) has recommended that the actual or future benefits discussed should not serve as an
inducement to participation. Nor should there be any financial gain from participation in genetics
research. This does not exclude, however, the possibility of reimbursement for an individual's time,
inconvenience and expenses (if any), even if there is a general distribution of benefits to the community. This is an issue that needs to be worked out before the trials begin.
In the case of trials that might be linked to profit-making endeavours, a percentage of the net profits(after taxes) should be donated to all members of the community in the geographical region because
they all participated in the research. Limiting the returns to only those leaders who gave consent for
their community to participate could create divisions within a group, is inconsistent with solidarity,and could be considered a bribe. The benefits of such trials could include health care infrastructure,
vaccines, tests, drugs, treatments, or other humanitarian efforts. While projects on vector control for
public health might not initially be seen as profit-making, the results and techniques developed could
be of potential profit to agriculture and public health in communities with the ability to pay for related technology. This issue has been discussed widely for human population genetics research (Chee etal., 1996).

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5 PUBLIC ATTITUDES TO GENETICALLYMODIFIED ORGANISMS
5.1 Need for public acceptance prior to interventions
One of the major driving forces of TDR is said to be the ability to bring together a large number ofpartners, donors, researchers and developers, from the North and South, and to catalyse processes tosolve public health problems and build research capacity. The goal of the project on social, economic
and behavioural factors is to bring in the public at all levels, including the local community, as fullpartners.
The nature of the work on public health interventions for major diseases necessitates involving several partners, and it is necessary to ensure that all partners maintain core ethical standards. If there isa breach of ethical practice, all partners will be criticized. WHO has not always been applauded for its
efforts to reduce tropical diseases since the 1950s. However it is widely recognized that WHO is technically the most competent organization with the highest moral authority for effecting interventions
to control diseases (Balter, 2000). Opinion surveys show that when it comes to biotechnology, theUnited Nations organizations, such as WHO, are the most trusted organizations and regulatory authorities in the world, while only a fraction of people trust their own government or industry (Macer et al.,1997).
In India in the 1970s a WHO/Indian Council for Medical Research project on various forms of SIT formosquito control was stopped after six years work because a journalist and the Parliamentary Public
Accounts Committee claimed that the intention was to research not new methods of vector control,
but biological warfare. These claims were echoed by Hanlon (1975), but his article was criticised by
Curtis & Curtis (1976). A point-by-point rebuttal of the claims was published by WHO (1976). A 3 page
editorial in Nature, in 1975, stated that the claims about biological warfare were “tenuous in theextreme” but made the fair criticism of WHO that it should have been more open with the media andtaken more time and trouble to explain the work of the unit and answer the journalist’s concerns. This
advice should be taken very seriously by future researchers with genetically manipulated mosquitos.
The fears of biological warfare have continued. For example, in India there were concerns that strainsof cholera found in an outbreak in the late 1990s were more consistent with introduced strains thanlocal strains, and biological warfare concerns were also expressed in regard to the proposed Human
Genome Diversity Project in the 1990s (Chee et al., 1996). The 2001 bioterrorism scare involving
anthrax in the United States has increased global fears of biological warfare, and because some books
on biological warfare have noted that mosquitoes would be good as vectors of biological warfare (BW)agents, it is likely that these concerns will continue. Any project that involves GM mosquitoes will haveto be clearly separate from BW research. However, the data gathered in field trials of GM insects will
be useful for any purpose, to some degree, and this calls for careful choice of research partner so as
not to be associated with researchers linked to BW research projects. Potential ethical issues may arise
if results from field trials provide a particular advantage for offensive use of BW as opposed to defensive use, but that is not currently foreseeable and all information should be published openly. There
have been times when some countries, e.g. USA, have restricted export of biotechnology knowledge forfears of misuse of that knowledge. However, in opinion surveys that ask open questions over people's
concerns about applications of genetic engineering, biowarfare is cited by only 1-2% (Macer, 1992;
1994). We could expect that, subsequent to the terrorist attacks in the USA on 11 September 2001,
the use of anthrax-containing letters has increased this concern.
In the past in Africa, some trials to introduce competitor insects resulted in the fear that larger-sizedmosquitoes were worse for people than small ones, even though the bigger mosquitoes were not vectors of human disease but were intended to be ecological competitors. Thus, education is needed

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before introducing an intervention. The local beliefs of communities need to be studied so that they
are documented and known, so that programmes can utilize local knowledge to help educate people
and design interventions that will be socially accepted. An intervention that is not socially accepted
is not likely to be successful.
Strategies for social behaviour change to reduce vector populations and transmission of disease have
had mixed results. Improper waste management and disposal systems, unplanned building activities,
stagnant drains, waste management problems, have all been cited as reasons for continued high vector populations. While public education needs to continue in attempts to find low-technology solutions
to these problems, the mixed experience suggests that social interventions alone will not be sufficient
to reduce vector numbers.

5.2 Results of public opinion surveys on genetically modified organisms
Although there have been numerous public opinion surveys on the release of different GMOs, there havebeen few surveys asking people their views on introducing GM vectors or pathogens for disease control. One general feature of the surveys is that GM plants are considered less threatening than GM
microbes, animals and humans (Macer, 1992). In the Asian region however, there is significantly lessconcern in India, China and Thailand than in Australasia, Japan, the Philippines and Russia (Macer,
1994; Ng et al., 2000). It is a question for further research whether there are differences between countries in the way they balance the benefits and risks of biotechnology.
In a survey conducted at the end of 2002 in Japan I asked two questions relating to GM insects to arandomly selected national sample. One third thought it would be acceptable to use genetic engineering to make mosquitoes unable to be a vector for human diseases like malaria or Japanese encephalophy, and only 16% said it would not, while half said they did not know. There was 54% approval forenvironmental release of mosquitoes that do not transmit human disease, which is the same as the support for release of GM disease resistant crops, with 19% disagreeing.
Among a series of specific examples of GMOs to be released into the environment (Macer, 1994), more
public in most countries supports release of bacteria to clean oil spills than to modify plants and animals, suggesting that, despite general concerns over microorganism release, if the purpose is considered worthy, people will be supportive. The majority of concerns people have are of health impact on
their family and themselves, fears that the technique is inherently unnatural, lack of trust in the system of safety assessment, and general fear of the unknown. Fewer people mention environmental concerns.
Although knowledge is important for acceptance of biotechnology, it is not a predictor of acceptance.
In surveys of scientists and the public in Japan in 1991-2000, for example, well-educated scientists
were often just as sceptical of biotechnology as the general public, and shared the same types of concerns (Macer and Ng, 2000). Opinions towards applications of biotechnology are formed by a variety ofbodies in society, and have been surveyed in many countries (European Federation on Biotechnology,1999). Episodes such as the human transmission of bovine spongiform encephalopathy (BSE), whichcauses human deaths from Creutzfeldt-Jakob disease in Europe, have shown that government scientists
are not always to be trusted to discuss risks (Dealler, 1995). The failure of the government authorities
led to higher public trust in NGOs, including environmental groups such as Greenpeace. The media has
also disproportionately reported negative aspects of genetic engineering because these appeal to people (Durant, 1995). Thus the late 1990s saw a dramatic drop in public support for biotechnology inevery country surveyed. It is therefore important that scientific knowledge be accurately shared with
all, that this process be open, and that all opponents are involved in discussion.
All these concerns can be the subject of better information and education. Gathering satisfactory scientific data by conducting field trials, and understanding ecological issues (Scott et al. 200), are themain criteria for most people. The remaining concern, and one which is also found in scientists as wellas the public, is that genetic engineering is somehow unnatural. This is an issue that needs greater

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social discussion. However, if presented with the threat of contracting disease, most people have few
concerns about using other "unnatural" remedies such as pesticides and medical drugs. Given that mostmosquitoes do not transmit disease to humans, it is, arguably, not unnatural to change a mosquitothat does transmit diseases into one that does not. There is a need for public opinion studies in thecommunities before the release, during the process of community engagement, and after the study, if
we wish to really understand the opinions and concerns that people have.

5.3 Wide social and legal discussion of biotechnology
Animal diseases are also transmitted by insect vectors. Biological control programmes have long beenused in agriculture, and there has been some discussion of introducing novel insect species into theenvironment in biological control. Although use of genetic engineering is more recent, already significant work has been conducted by many organizations on risk communication and public acceptanceof GMOs, especially in the food and agriculture sectors. Among the UN bodies, FAO has committees on
biotechnology, bioethics and biosafety, which may be potential partners for future collaboration about
introducing GM vectors.
The positive and negative aspects of genetic engineering are discussed in a wide variety of publications. Many popular books are negative in their portrayal (Ho, 1998; Wheale et al. 1997; Rifkin, 1998),
while a few are positive or neutral (Macer, 1990; Reiss and Straughan, 1996; Bruce and Bruce, 1998;
Comstock, 2000). The ethics of technology that controls reproduction has mainly been considered forplants, but a 1998 FAO conference dealt with animals and the use of terminator technology. The ethics
of reproductive and transgenic technology in land and marine animals has also been discussed,
although less so for insects.
Particular ethical concerns have been raised over animal cloning. International instruments have dealt
with human cloning, such as the ban imposed by Article 11 of the United Nations Educational,
Scientific and Cultural Organization (UNESCO) Universal Declaration on the Human Genome and Human
Rights (1997), but this Declaration did not mention animals. The HUGO Ethics Committee Statement
on Cloning (1999) recommended that "animal cloning be subject to the same principles concerning
animal welfare as other experimentation on animals". It also says "Regard should be had to possible
consequences for biodiversity". Cloning technology is now being applied to animals for food production and use in research (Nilson, 1997).

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6 REGULATIONS
6.1 General basis for regulation
In the TDR strategy for 2000 to 2005, it is stated that research and development of means to combat
disease and improve health must adhere to internationally accepted legal and ethical principles (TDR,
2000b). The internationally accepted principles of risk assessment for GMOs take into account: relevant technical and scientific details of the recipient or parental organism, the donor organism(s), thevector, the insert(s) and/or characteristics of modification, the GMO, and the methods for detection
and identification of the GMO including specificity, sensitivity and reliability; as well as information
relating to intended use, information on location and geographical, climatic and ecological characteristics, and the foreseen health impact of the intervention. The ethical principle of non-maleficence isthe underlying basis for attempting to avoid harm and the regulation of human activity.
What is a particularly relevant point in the development of GM vectors and pathogens for disease control, that distinguishes them from GMOs for agricultural use and food production, is that in order fora vector programme to be successful, the modification must spread throughout the wild population ofa vector. This means that deliberate infection with the transgene may be the target of introducing the
GMO. In order to define the parameters associated with the speed and extent of spread of the genetic
modification under real conditions, extensive trials are necessary. Some vectors may transmit more than
one pathogen, so any intervention programme may have complicated effects on the distribution of disease. More detailed examination may be necessary before introducing GM pathogens themselves. The general principles of biosafety have always attempted to contain pathogens, with the exception of vaccines.
Decision-making can be contrasted between legalists, who strictly follow guidelines or laws, and situationalists, who use principles to guide case-by-case decision-making (Fletcher, 1966). Often a caseby-case approach is necessary, so committees are needed to interpret the rules. Thus ethics committees are a useful way to implement guidelines, and transparent ethics committees should be established locally to review research trials.
The term international implies that a cross-cultural approach should be used for setting standards. The
term cross-cultural includes both a broad representation within one society and a transnational
approach. Even in the case of a trial on an isolated island of a single sovereign state, if it involves
TDR, it should utilize international expertise. If a trial fails in one site, the failure could hold up
progress internationally for years due to negative public images. Thus a trial of a GM vector is not justa national issue.
Once we accept that trials will necessitate international approaches and collaboration, we can ask whatis the appropriate international forum to examine the safety of GM pathogens and vectors. Concern
over possible safety and environmental risks raised by biotechnology prompted the WHO, United
Nations Environment Programme (UNEP) and United Nations Industrial Development Organization(UNIDO) to identify and study the various safety issues involved. As a result, a UNIDO/UNEP/WHO/FAO
Ad Hoc Working Group was formed in 1990 to work out practical guidelines through a series of consultations with international experts and scientists from developing countries. In 1991, the
UNIDO/UNEP/WHO/FAO Working Group on Biosafety brought out a Voluntary Code of Conduct for theRelease of Organisms into the Environment. The code sets out general principles and a framework andguidelines to be adopted at national, regional and international levels to facilitate the safe application of biotechnology. The scope of this document covers "GMOs at all stages of research, development,
use and disposal, while focusing on release to the environment. It covers, but is not limited to, genetically modified plants, animals (including for example, insects, molluscs and fish), and microorganisms
and their products and by-products".
The International Centre for Genetic Engineering and Biotechnology (ICGEB) provides assistance inbiosafety training for the development of genetic engineering in many countries (ICGEB, 2002). Some

Ethical, legal and social issues of genetically modified disease vectors in public health27issues also relate to the proposed Code of Conduct in Biotechnology being developed under theCommission on Genetic Resources for Food and Agriculture (CGRFA). UNDP and FAO generally support
the development of genetic technology while considering the benefits and risks of the organisms. The
capacity of countries to establish committees to adequately address ethical, social and scientific concerns needs to be strengthened.
The Scientists' Working Group on Biosafety of the Edmonds Institute (1998) in Washington D.C., USA,recommended that field trials of vectors genetically engineered to reduce disease should be small scalein terms of the area of dispersal of the vector. "In the case of an anti-malaria or anti-dengue intervention, such a field trial could involve a single village or an isolated cluster of adjacent villages. No
large-scale release should be attempted until the effectiveness is shown in the first trial". Thus, while
there is general international consensus in the UN system that selected use of GMOs should proceed,
there are groups within society that continue to be cautious. There are also countries whose politicalregimes do not accept GMOs, and these attitudes depend on political elections, including the principleof democracy. National sovereignty should of course be respected, but GM vectors may spread beyond
a national border

6.2 Cartagena Protocol on Biosafety
The Cartagena Protocol on Biosafety to the Convention on Biological Diversity is an advance informed
agreement procedure on the safe transport, handling and use of living modified organisms resulting
from modern biotechnology that specifically focuses on transboundary movements of living modified
organisms. The parties to this protocol agreed to ensure that "the development, handling, transport,
use, transfer and release of any living modified organisms are undertaken in a manner that prevents or
reduces the risks to biological diversity, taking also into account risks to human health". It was also
noted that "the parties are encouraged to take into account, as appropriate, available expertise, instruments and work undertaken in international forums with competence in the area of the risks to humanhealth" (CBD, 2000).
In the Cartegena Protocol, "a living modified organism means any living organism that possesses anovel combination of genetic material obtained through the use of modern biotechnology. Modern
biotechnology means the application of either in vitro nucleic acid techniques, including the recombinant DNA and direct injection of the nucleic acid into cells or organelles, or the fusion of cells beyond
the taxonomic family, that overcome natural physiological reproductive or recombination barriers andthat are not techniques used in traditional breeding and selection". This definition of a living modified organism (LMO) may be relevant to future projects of TDR as it becomes accepted in international law in general because of the Protocol. It is being adopted in documents from other UN bodies, suchas those of the Codex Alimentarius Commission. The actual term "living modified organism" is still not
as widely used as "genetically modified organism", the term that has been used for two decades inacademic and media debates.
If the vector, symbiont or pathogen is a LMO, it would be covered under the Protocol. Because of additional regulatory considerations, some developers may wish to develop pathogens or vectors carefully
so they would be deliberately excluded from the provisions of this Protocol, which could be done bymaking sure the products and processes used do not come under the category of modern biotechnology for the purposes of the Protocol. For example, sterile insects and vectors with chromosomal or genedeletions produced in traditional ways or by traditional ways of selection, may not be considered as
LMOs for the purposes of regulation. However, a responsible programme would want to cover any
process used to modify the vector so that the vector (product) itself is carefully examined, not just theprocess of production. One important exclusion of the Cartegena Protocol is in article 5, which states,"the Protocol shall not apply to the transboundary movement of living modified organisms which arepharmaceuticals for humans that are addressed by other relevant international agreements or organizations".

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One useful development of the Cartegena Protocol umbrella is the establishment of biosafety clearing
houses, which are contact points in each member country. The Protocol also includes risk assessment
and risk management once agreement is reached, as well as development of capacity building inbiotechnology research. Many developing countries do not have the economic or scientific capacity
needed to examine the products of modern biotechnology (Chinsembu and Kambikambi, 2001).
Information related to GM vectors should be linked to the same biosafety clearing houses.

6.3 National and regional regulations
General guidelines for working with recombinant organisms exist in many countries, and international
assistance through the Organization for Economic Cooperation & Development (OECD) and UNIDO isavailable for countries that do not have regulations. Some countries have already established and published guidelines for the safety assessment of genetically modified insects. The international regulations are important as models for countries that lack the means for considering the details on theirown.
The United States Department of Agriculture (USDA) has reported the results and approval proceduresfor six trials of genetically modified organisms. These include field trials of transgenic mites, nematodes, flies, spruce budworm, and pink bollworm, as discussed above (USDA, 2002). The field trials oftransgenic nematodes and predatory mites were intended to study the risk to the environment of thesetransgenic organisms and the stability of the transgenes under controlled conditions. The genetic modifications did not affect infectivity of the nematodes, however the field performance of the transgenic
mites (in Florida) was dramatically different to in the laboratory due to differences in relative humidity, and the field experiment was terminated after three weeks because populations of both predatory
mites and prey spider mites declined rapidly. Few individuals in the population contained the transgene. At the end of these field trials, all the transgenic organisms and hosts were destroyed.
Like most medical associations, some professional scientific organizations have ethics codes (e.g. theAmerican Anthropological Association, 1998). More specific to transgenic insects in the USA, Arthropod
Containment Guidelines were developed by the American Committee of Medical Entomology (ACME,
2000), part of the American Society of Tropical Medicine and Hygiene. If the transgenic arthropod is
assigned to a risk level in the US public health services’ Biosafety in Microbiological and Biomedical
Laboratories, then institutional biosafety committee approval is required. If the field study involves
humans, IRB oversight is necessary. IRBs in the USA require each human subject in a field trial to giveinformed consent to be involved in the project, as discussed in section 4. This example may be usefulas a model for developing international guidelines, and for countries developing their own guidelines.
A few local communities have established GM free areas, but this may require specific national legislation. Australia allows local communities to declare GM-free areas, and in a sense this is consistent
with the ethical underpinning that leads to calls for community consent. It is an issue that needs tobe considered in the field in particular cultural and political frameworks. If a specific sector of a community has a medical need, their claim to be free of risk of disease does need to be taken into account
if other members of the community have blocked efforts towards new strategies that might reduce thisdisease burden.

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7 RECOMMENDATIONS FOR KEY AREAS TO
ENSURE ETHICAL INTEGRITY IN THE USE OF
GENETICALLY MODIFIED VECTORS AND PATHOGENS
7.1 Establish safety and international standards before field trials
There may be a need to establish an international safety code under TDR. Because of limited resourcesand experience, there is a need for policy guidance for ethical genetic engineering of microorganisms,
plants, animals and ecosystems in each country before release of GMOs. Policy advice in each country
should be the product of open social dialogue including all sectors of society. That the process of social
debate needs to be held before releasing GMOs is a lesson learned from countries that have attempted to use GMOs before wide social discussion took place. In addition, an international approach is
required since vectors do not honour national borders, nor is their behaviour always predictable.
Although some international NGOs take a very negative view towards genetic engineering, they do provide a useful sounding board for governments so that a thorough examination of the issues is madebefore GMO release. However, given the international consensus that some GMOs are safe to release asargued by UNDP and FAO, and the fact that there were already 50 million hectares of land under genetically modified plants in 2001 (James C, 2001 [a], 2001[b]), the question is not whether to release
GMOs but how to release them, and what type of GMOs are safest and most effective.
Part of the process is for a society to set values for consensus on risk assessment. There is a need tofind a universal minimal standard of risk assessment as diseases and vectors cross national and continental borders. The following areas need to be considered in developing model guidelines:• Before field release of transgenic organisms, researchers must assess all the scientific and social
issues associated with GM vectors and develop safety precautions to address potential risks.
• The scientific and social risks should be minimized through careful design of the vector system, relevant laboratory experience, and careful choice of site including consideration of appropriate socialand cultural factors.
• Even if there are no perceived realistic risks, a procedure for their evaluation should be set up sothat new information can be gathered and interpreted. This procedure may involve establishing a
specialized ethical review committee under TDR auspices to offer advice to researchers seeking guidance on the ethics of projects.
• There should be prior environmental, medical and social studies for site selection, and the mostappropriate site should be chosen based on the data obtained (see section 7.4).
• Information should be openly exchanged as broadly as possible to relevant community leaders,members of the community, and mass media. This needs to be done with international collaboration(see section 7.2).
• Consent should be obtained from the communities involved (see sections 4.2, 7.3). Specific mechanisms for this need to be developed and will be useful for other areas of public health interventions.
• A contingency plan for aborting field trials needs to be developed. One approach is to engineer alethal gene for the vectors that can be induced by a non-toxic chemical to ensure total eliminationof those that have acquired the genetic construct.
• Commitment to the local communities involved in field trials should be made that they will be thefirst beneficiaries of more permanent use of a GM vector should the results indicate that its use isappropriate.

Ethical, legal and social issues of genetically modified disease vectors in public health31• Intellectual property concerns should not be barriers to implementation of public health measuresusing GM vectors or their symbionts and/or pathogens. Prior negotiation, including possible involvement to allow access to the latest technology, is preferable to confrontation.
• To avoid any suspicion by the public that could result in their rejection of this approach, TDR andmember governments should not involve partners from military research establishments in the projects.
• The data should be open to all in order to benefit from global expertise and develop international
consensus.
• Whatever guidelines are developed, they should be revised as experience with genetic engineering
technology grows, as knowledge of ecology and communities grows, and with societal trends.

7.2 Information access and local communities as partners
Numerous cases, from the termination in the 1970s of a WHO-sponsored release programme of chemically sterilized mosquitoes in India, to the international GM debates in the late 1990s, show the needfor adequate information to be provided well before implementing genetic technology. In the interestsof justice, the last decade has witnessed an emerging international consensus in many fields of researchthat communities participating in research should, at a minimum, receive some benefit. A benefit is agood that contributes to the well-being of an individual and/or given community (e.g. region, tribe,
disease group) (Knoppers et al., 2000); it transcends avoidance of harm (non-maleficence) by promoting the individual’s and/or community’s welfare. Thus, a benefit is not identical with profit in the monetary or economic sense - some benefits are monetary, others are not. Determining a benefit depends
on needs, values, priorities and cultural expectations.
Benefit is seen as the eventual prevention or treatment of a disease. Prior consultation with individuals and communities, and their involvement and participation in the research design, is a preliminary
basis for future distribution of the benefit, and may be considered a benefit in itself. Better information is clearly a benefit and may be linked to better compliance and/or use of other methods of disease prevention.
Provision of information through the Internet allows many people who are not policy-makers to haveaccess to basic information about projects involving GM vectors. Disseminating information in this way
can be effective in resource-poor communities, but is not a substitute for finding good local communicators to spread the information at public meetings and provide written and pictorial descriptions of
the reasons for the project. Not only is there the problem of Internet access but also the difficulty ofunderstanding. Grass-roots initiatives can lead to innovations and pressure upon regulators to change
policy, as seen in the history of agricultural interventions in developing countries.
We can envisage several types of scenario. One is technology transfer where a local community decides
to embark on its own modification of a vector or pathogen. A more advisable policy, because of thedangers of lack of quality control in the modification event, is local production of GMOs that havealready been well characterized in scientifically conducted field trials.
Even in communities without established political structures for public participation, local consumers,
women’s and mother's groups, etc., may be able to exert political pressure on policy-makers to introduce GMOs into their communities if they are made aware of the potential benefits to save their children's lives. This principle of empowerment is ethically consistent with WHO policy, but could at times
lead to conflict if a particular country has already rejected such an intervention at political level.
However, women have been distanced from decision-making in most societies (Sherwin, 1992).
Rather than conduct a public opinion survey on the acceptance of GM vectors before it is clear what
the specific proposal is, interviews and surveys may be included in educational efforts and priorinformed dialogue with local people about the proposal. This should be a two-way educational process

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at person-person level in the field, starting with professionals (nurses, doctors, teachers), who theninform their local networks.
People who want extra information can access it through the TDR website and publications. Exercises
should be developed for school students, to help them consider ethical and scientific issues, and develop maturity for making decisions they face in the technical age. International partners might include
UNESCO, the International Union of Biological Sciences (IUBS), and other bodies attempting to develop bioethics education. The relevance of projects to eradicating disease in a community may act as a
trigger to motivate people to become involved in the community engagement process, even if thecommunity has not had the motivation in the past.

7.3 Group consent
Recognizing the autonomy of people as a group demands that we apply the consent model to morethan isolated individuals. The introduction of GM vectors and pathogens requires community consent,
so a process for seeking group consent needs to be developed for each community (Kleinman, 1999).
There are some parallels with seeking group consent from population groups that may be asked to giveDNA samples for population genetics research (Greely, 1996; Macer, 1997). The problem is, who represents a community? Is it the political or religious leaders? Another issue that needs to be investigated is the appropriate age of consent in different societies. The more inclusive the process the better.
There is inadequate discussion of this procedure to date, and it is a key area for further discussion inorder to decide the ethics of such interventions.
The changing economic, social, political and civil structures have implications for the concept of groupor community consent to release of modified vectors or pathogens. There are also legal implications in
those countries with laws such as patient's bill of rights (Annas, 1989). The question of whether every
citizen has to consent to public health interventions is not a new one (Kass, 2001), but with the current social transition from a paternalistic society to informed consent and informed choice, this key
concern is appearing in all societies, although at different speeds.

7.4 Environmental assessment
This area would contribute to more detailed environmental and social impact guidelines for TDR projects. Environmental impact data need to be collected from long-term studies so they are not subject
to climatic variation. It may be useful to start environmental monitoring several years before the introduction of a GMO in target areas so there are local background data to compare future interventions
with and to provide scientific accuracy for people to make informed choices.
The ecological and genetic lessons learnt from agricultural studies will be important for future publichealth applications of GM vectors. Concerns about the environmental safety of GM trials have been
expressed by numerous writers (Ho, 1998; Rifkin, 1998) and various groups, e.g. the Union ofConcerned Scientists (2001).
Regarding the environmental behaviour of modified vectors, many generations of vectors need to bestudied in the laboratory to test gene stability before they are introduced in small field trials (Scott etal. 2002). If possible, there should be an initial trial on a sparsely inhabited island. To adequately test
the vector however, there need to be controlled trials in disease endemic areas.
Internationally, a large amount of biosafety research on GMOs has been conducted in connection withhuman health and the environment, although there is still a need for international cooperation in
research on environmental aspects. There needs to be consensus on the time needed for long-term
studies, and for coordination and discussion of what amounts to genetic "pollution". Thresholds for
acceptable levels of gene flow or ecological pertubation need to be established, and data and experience gathered from studies in countries (with UNEP, UNIDO) under a wide range of environmental con-

Ethical, legal and social issues of genetically modified disease vectors in public health33

ditions. International guidelines and assistance in monitoring field trials of GMOs, including after widespread release has been approved, are urgently needed. Decisions as to what outcome measures aresocially and scientifically appropriate, and what methodologies are appropriate to enable this in a way
consistent with ethical principles such as beneficence and respect for persons should involve TDR.
Because of the inherent uncertainties of ecology and societal stability, it is imperative that each intervention is tested under a range of ecological and social conditions, and that data are stored and sharedin a database.
The range of ethical issues and public concerns will not be significantly different whether we are considering GM vectors or pathogens. The approach with the lower overall environmental risk is to be preferred so that research into different mechanisms is simultaneously stimulated. In both cases there aresome species-specific concerns.

7.5 International cooperation
Knowledge about ethical issues in connection with GMOs should be expanded by asking member countries to contribute information and enter into dialogue on the issues. The network of contacts, including bioethics institutes, civil society organizations (CSOs)/NGOs, and consultants, can compile relevantfacts and values. Molecular entomology has not been discussed amongst the international bioethics
community, and there is a need for discussion of the issues at appropriate forums and conferences.
WHO is the joint partner with FAO in international work on safety, e.g. food safety in CAC, animal diseases (there is joint FAO/Office international des epizooties (OIE)/WHO reporting of notifiable animal
diseases), and other public and occupational health initiatives. UNEP works on a number of environmental ethics issues indirectly, and the Cartegena Protocol will be applicable to many TDR projects.
UNESCO has the COMSTECH committee and the International Bioethics Committee, which also look at
the ethical issues raised by genetic engineering. Some of the broader ethical issues are being discussed
in these forums, and collaboration should help bring in a wider range of viewpoints to take a more
holistic approach than merely the human health aspects of interventions for which WHO has primary
responsibility. The other international bodies working on bioethics include the Council for International
Organizations of Medical Sciences (CIOMS), the World Medical Association (WMA), the HUGO Ethics
Committee, ethics committees of professional associations, the International Association of Bioethics,
to mention just a few. There are numerous regional and national associations of bioethics.

7.6 Further next steps in consultation on ethical, legal and social issues
This report is only one step in considering ethical issues. Already there have been social studies of theimplementation of TDR projects for disease control, which show that there is variation in the uptakeof interventions depending on the community and disease involved (TDR 2000[a]; 2002). In addition
to the recommendations above, the following steps need to be effected concurrently and urgently.
(a) Gathering of descriptive data on member countries approaches and values
Data should be gathered on a continuous basis. There are still unknown factors in the way societies
apply various types of ethical theories and principles to different aspects of life, so general descriptive
ethics studies in the countries that may be likely targets would be useful for developing general social
science approaches. More detailed surveys on focused issues may be useful when a project is getting
close to the field study stage. Member countries of WHO/UNDP/World Bank could be asked directly toprovide information and comments, which would then need to be analysed. The gathering and reporting of data can make countries more conscious of the issue, and may encourage local researchers to
explore community attitudes to GM vectors. Some of these concerns may be useful for the design stageof vector programmes, so a general call for comments should be made allowing enough time for focused
attention on new areas that arise. Help is needed in gathering data by sound social science methodologies in many countries.

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(b) Interactive academic and public forums for debate
Different forums have distinct purposes. Intergovernmental forums aim at consensus, but this may not
be necessary as already governments have agreed that combating tropical diseases is a major priority,that biotechnology is one means to do this, and that this needs to be well-regulated. However, for specific target areas when close to field implementation, multilateral consultations should be held withneighbouring jurisdictions to prepare for contingencies in case of a vector migrating across nationalboundaries.
Another type of forum should specifically aim at identifying the differences and diversity in world views
that may affect the way scientific facts are interpreted, by inviting different communities that may beaffected, interested civil society organizations (CSOs) (both not-for-profit and for-profit), and experts
of diverse opinion. This identification of diversity of thought is also important as part of a systematic approach to ethical analysis. This would allow preparation of adequate responses to all points ofview, and allow a chance for gathering descriptive information through forums where persons argue fordifferent approaches.
These issues should be discussed in the bioethics community, in regional and global meetings, so thatthe full range of the global bioethics community is given a chance to participate. This will also aid
information dissemination as case studies will be carried back to different universities and schools formore people to reflect on. Experience of the way science and technology ethical and social issues werediscussed by teachers in many countries before introducing curricular changes (Macer et al., 1996) suggests that, if members of a community perceive a need as individuals, they will become involved in
the process. TDR could utilize the individual initiatives of interested persons by providing information
kits and access to information, and developing networks to encourage them.
The TDR partners have a duty to individual people, individual countries, member countries as a whole,and humankind as a whole. The process of dialogue and negotiation can be a more ethical option thana prescriptive stand if we consider the autonomy of those involved and the consequences. The process
of forum is essential for developing methods to obtain group and community consent for public healthinterventions in general, and in particular for this project. The implications of informed consent for
public health projects have not been adequately developed.
(c) Development of model guidelines
Many have called for an international guideline. Whether or not the guideline is legally binding, likethe Cartegena Protocol, a consensus text is useful as countries attempt to face these issues with a lackof previous experience. The text should be accompanied by detailed explanation of the process leading up to the text so that each country can explore the arguments behind the guideline and see what
is most appropriate for their society. There will still be a fear that companies or governments may go
ahead with projects whether or not the guideline is followed, and whether or not they seek advice from
TDR. An international secretariat, located in TDR, could be established to coordinate information fromfield trials. This secretariat could give advice on all aspects outlined in section 7.1, and this could consider ethical and social factors as an integral part.
(d) Education on the guidelines, and feedback
Once model guidelines are developed, it will be important to circulate them for comments. This may
be the best stage for an active education process, as it will educate scientists about the guidelines andeducate the general community about the mechanism established for field trials. Broad education to
open the minds of people, at all levels of society, to the options available for disease control and how
to make better decisions for themselves, is considered part of the empowerment of citizens. The process
should not just be one way, but interactive and combined with the recommendation to gather descriptive data.

Ethical, legal and social issues of genetically modified disease vectors in public health

35

TDR is called upon to take the initiative in coordinating these programmes because release of GM vectors is a question for the global community, and the consequences will potentially impact on many generations to come. There is a moral mandate for these studies to progress rapidly, but carefully.

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ACKNOWLEDGEMENTS
The useful and critical advice of WHO staff is gratefully acknowledged, in particular that of Dr Boris
Dobrokhotov, Dr Nina Mattock, Ms Kalyani McCullough, Dr Carlos Morel, Dr Ayoade Oduola, Dr Johannes
Sommerfeld, Dr Yeya Touré and Dr Daniel Wikler. The useful suggestions of reviewers and conference participants in several countries who gave feedback on the ideas are also warmly acknowledged.

Ethical, legal and social issues of genetically modified disease vectors in public health

37

ABBREVIATIONS

38

ACME

American Committee of Medical Entomology

BW

Biological warfare

CAC

Codex Alimentarius Commission

CBD

Convention on Biological Diversity

CGRFA

Commission on Genetic Resources in Food and Agriculture

CIOMS

Council for International Organizations of Medical Sciences

CSO

Civil Society Organization

FAO

Food and Agriculture Organization

FDA

United States Food and Drug Administration

GFP

Green fluorescent protein

GM

Genetically modified

GMO

genetically modified organism

HUGO

Human Genome Organization

ICGEB

International Centre for Genetic Engineering and Biotechnology

IPGRI

International Plant Genetic Resources Institute

IPRIntellectual property rights

IRB

Institutional review board

ISAAA

International Service for the Acquisition of Agri-biotech Applications

IUBS

International Union of Biological Sciences

LMO

Living modified organism

NGO

Non-governmental organization

OECD

Organisation for Economic Co-operation and Development

SIT

Sterile insect technique

TDR

UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases

TRIPS

Trade-Related Intellectual Property Rights

UN

United Nations

UNDP

United Nations Development Programme

UNEP

United Nations Environmental Programme

UNESCO

United Nations Education, Scientific and Cultural Organization

UNIDO

United Nations Industrial Development Organization

USDA

United States Department of Agriculture

USDOE

United States Department of Energy

WHOWorld Health Organization

WTO

World Trade Organization

Social, Economic and Behavioural Research • Special Topics No. 1

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