08/02/16

KIT COMPONENTS
Product code
425312F
425314F

Product name
N-Histofine® DAB-2V

SDS-ID

Component nameF-1000DAB Reagent A

F-1003DAB Reagent B

Safety Data Sheets are provided on the next page.

NICHIREI BIOSCIENCES INC.

Safety Data Sheet
Material Name: DAB Reagent A

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended* * *Section 1 - IDENTIFICATION OF THE SUBSTANCE / MIXTURE AND OF THE COMPANY /UNDERTAKING* * *1.1 Product Identifier:Product Identifier: DAB Reagent A
Substance Registration Number(s)This product and its components are not subject to REACH.
1.2 Relevant Identified Uses of the Substance or Mixture and Uses Advised Against
Identified Uses
immunohistochemical use
Reagent type: Bottle
425312F: -Histofine DAB-2V ; DAB reagent A; 1.2 ml x 2425314F:-Histofine DAB-2V ; DAB reagent A; 1.2 ml x 6

Uses Advised Against
Restricted to professional users. Cannot be distributed to the general public.
1.3 Details of the supplier of the safety data sheet
Nichirei Biosciences Inc.
6-19-20, Tsukiji, Chuo-Ku
Tokyo, Japan

Telephone number: +81-3-3248-2207
Email: n1060x005@nichirei.co.jp

1.4 Emergency Telephone Number+81-3-3248-2207 (9:00-17:00 Japan time)* * *Section 2 - HAZARDS IDENTIFICATION* * *2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No 1272/2008
Flammable liquid, Category 2
Acute Toxicity (Dermal), Category 3 (2.5% unknown)
Acute Toxicity (Oral), Category 4 (2.5% unknown)
Acute Toxicity (Inhalation), Category 4 (2.5% unknown)
Serious Eye Damage/Eye Irritation, Category 2AGerm cell mutagenicity, Category 2
Carcinogenicity, Category 1A
2.2 Label Elements
Labelling according to Regulation (EC) No 1272/2008:
Symbol(s)Signal Word____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedDANGER
Hazard Statement(s)H225 Highly flammable liquid and vapour.
H311 Toxic in contact with skin.
H302 Harmful if swallowed.
H332 Harmful if inhaled.
H319 Causes serious eye irritation.
H341 Suspected of causing genetic defects
H350 May cause cancer.
Precautionary Statement(s)Prevention
P210 Keep away from heat/sparks/open flames/hot surfaces. - No smoking. P201 Obtain special instructions
before use. P280 Wear protective gloves/protective clothing/eye protection/face protection.
Response
P308+P313 IF exposed or concerned: Get medical advice/attention. P303+P361+P353 IF ON SKIN (or hair):Take off immediately all contaminated clothing. Rinse skin with water/shower.
StorageP403+P235 Store in a well-ventilated place. Keep cool.
Disposal
P501 Dispose of contents/container in accordance with local/regional/national/international regulations.
2.3 Other Hazards
None known.

* * *Section 3 - COMPOSITION / INFORMATION ON INGREDIENTS* * *
CAS
EC No
Registration No
75-05-8
200-835-2--

Component
Synonyms

1272/2008(CLP)

Percent

Acetonitrile

50

7732-18-5
231-791-2-7411-49-6
231-018-9
--

Water

Flam. Liq. 2
H225
Acute Tox. 4(Oral) H302
Acute Tox. 4(Dermal) H312
Acute Inh. Tox.
4 H332Eye Irrit. 2
H319--

Muta. 2 H341
Carc. 1A H350

2.5

3,3'-Diaminobenzidine tetrahydrochloride

47.5

CAS 7411-49-6: Self classification required____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended* * *Section 4 - FIRST AID MEASURES* * *4.1 Description of First Aid Measures
Inhalation
Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER or doctor/physician.
Skin
Wash with plenty of soap and water. Take off contaminated clothing. Get medical attention if irritation develops.
Thoroughly clean and dry contaminated clothing and shoes before reuse.
Eyes
Flush eyes with plenty of water for at least 15 minutes. Remove contact lenses, if present and easy to do.
Continue rinsing. Get immediate medical attention.
Ingestion
If swallowed, get medical attention.
4.2 Most Important Symptoms and Effects, both Acute and Delayed
Acute
eye irritation
Delayedcancer, mutagenic effects
4.3 Indication of any Immediate Medical Attention and Special Treatment Needed
Treat symptomatically and supportively. For inhalation, consider oxygen.

* * *Section 5 - FIRE FIGHTING MEASURES* * *5.1 Extinguishing Media
carbon dioxide, regular dry chemical, water spray, alcohol resistant foam
Unsuitable Extinguishing MediaDo not scatter spilled material with high-pressure water streams.
5.2 Special Hazards Arising from the Substance or Mixture
Highly flammable liquid and vapor. Vapors or gases may ignite at distant ignition sources and flash back.
Thermal Decomposition Products
Combustion: oxides of carbon, oxides of nitrogen, cyanides, hydrogen chloride gas
5.3 Advice for Firefighters
Fire Fighting Measures
Move container from fire area if it can be done without risk. Cool containers with water spray until well after thefire is out. Do not scatter spilled material with high-pressure water streams. Stay away from the ends of tanks. For
fires in cargo or storage area: Cool containers with water from unmanned hose holder or monitor nozzles until
well after fire is out. If this is impossible then take the following precautions: Keep unnecessary people away,
isolate hazard area and deny entry. Let the fire burn. Withdraw immediately in case of rising sound from venting
safety device or any discoloration of tanks due to fire. For tank, rail car or tank truck, evacuation radius: 800
meters (1/2 mile). Avoid inhalation of material or combustion by-products.
Protective Equipment and Precautions for Firefighters
Wear full protective fire fighting gear including self contained breathing apparatus (SCBA) for protection against
possible exposure.

* * *Section 6 - ACCIDENTAL RELEASE MEASURES* * *6.1 Personal Precautions, Protective Equipment and Emergency Procedures
Wear personal protective clothing and equipment, see Section 8.

____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended

6.2 Environmental Precautions
Avoid release to the environment. Prevent entry into sewers, drains, ditches, underground or confined spaces andwaterways.
6.3 Methods and Material for Containment and Cleaning up
Do not touch or walk through spilled material. Absorb with sand or other non-combustible material. Collect spilledmaterial in appropriate container for disposal. Use non-sparking tools and equipment. Large spills: Dike for later
disposal. Keep unnecessary people away, isolate hazard area and deny entry. Stay upwind and keep out of low
areas.
6.4 Reference to Other Sections
See Section 7 for Handling Procedures. See Section 8 for Personal Protective Equipment recommendations. See
Section 13 for Disposal Considerations.

* * *Section 7 - HANDLING AND STORAGE* * *7.1 Precautions for Safe Handling
Obtain special instructions before use. Do not handle until all safety precautions have been read and understood.
Keep away from heat, sparks, open flame, and hot surfaces - No smoking. Keep container tightly closed.
Ground/bond container and receiving equipment. Use explosion-proof electrical/ventilating/lighting equipment.
Use non-sparking tools and equipment. Take precautionary measures against static discharge. Avoid breathingvapor or mist. Use only outdoors or in a well-ventilated area. Do not eat, drink, or smoke when using this product.
Avoid contact with eyes, skin and clothing. Wear protective gloves/clothing and eye/face protection. Wash
thoroughly after handling. Avoid release to the environment.
7.2 Conditions for Safe Storage, Including any Incompatibilities
Store and handle in accordance with all current regulations and standards. Store in a well-ventilated place. Keep
container tightly closed. Keep cool. Grounding and bonding required. Store locked up. Keep separated fromincompatible substances.
7.3 Specific End Use(s)immunohistochemical use* * *Section 8 - EXPOSURE CONTROLS / PERSONAL PROTECTION* * *8.1 Control Parameters
Component Exposure Limits
Acetonitrile (75-05-8)
EU (IOELV):
Austria:

Belgium:
Bulgaria:
Croatia:

Denmark:

40 ppm TWA; 70 mg/m3 TWA
Possibility of significant uptake through the skin
40 ppm TWA [TMW]; 70 mg/m3 TWA [TMW]
160 ppm STEL [KZW] (4 X 15 min); 280 mg/m3 STEL [KZW] (4 X 15 min)skin notation
20 ppm TWA; 34 mg/m3 TWA
Skin
40 ppm TWA; 70 mg/m3 TWA
Skin notation
40 ppm TWA [GVI]; 68 mg/m3 TWA [GVI]
60 ppm STEL [KGVI]; 102 mg/m3 STEL [KGVI]Skin Notation
40 ppm TWA; 70 mg/m3 TWA____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

Estonia:

Finland:France:Germany (TRGS):

Gibraltar:
Germany (DFG):

Greece:Hungary:
Ireland:
Italy:

Latvia:Lithuania:
Luxembourg:
Malta:
Netherlands:
Poland:Portugal:

Romania:
Slovak Republic:
Slovenia:
Spain:SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedPotential for cutaneous absorption
40 ppm TWA; 70 mg/m3 TWA
60 ppm STEL; 100 mg/m3 STEL
Skin notation
20 ppm TWA; 34 mg/m3 TWA
40 ppm STEL; 68 mg/m3 STEL
Potential for cutaneous absorption
40 ppm TWA [VME] (restrictive limit); 70 mg/m3 TWA [VME] (restrictive limit)
Risk of cutaneous absorption
20 ppm TWA AGW (The risk of damage to the embryo or fetus can be excluded when
AGW and BGW values are observed, exposure factor 2); 34 mg/m3 TWA AGW (Therisk of damage to the embryo or fetus can be excluded when AGW and BGW values
are observed, exposure factor 2)
skin notation
Skin notation
40 ppm TWA; 70 mg/m3 TWA
20 ppm TWA MAK; 34 mg/m3 TWA MAK
40 ppm Peak; 68 mg/m3 Peakskin notation
40 ppm TWA; 70 mg/m3 TWA
60 ppm STEL; 105 mg/m3 STEL
70 mg/m3 TWA [AK]potential for cutaneous absorption
40 ppm TWA; 70 mg/m3 TWA
Potential for cutaneous absorption
20 ppm TWA Media Ponderata nel Tempo; 35 mg/m3 TWA Media Ponderata nel
Tempo
skin - potential for cutaneous absorption
40 ppm TWA; 70 mg/m3 TWA
skin - potential for cutaneous exposure
40 ppm TWA [IPRD]; 70 mg/m3 TWA [IPRD]
Skin notation
40 ppm TWA; 70 mg/m3 TWA
Possibility of significant uptake through the skin
40 ppm TWA; 70 mg/m3 TWA
possibility of significant uptake through the skin
34 mg/m3 TWA
70 mg/m3 TWA [NDS]
140 mg/m3 STEL [NDSCh]
40 ppm TWA [VLE-MP] (indicative limit value); 70 mg/m3 TWA [VLE-MP] (indicative
limit value)
skin - potential for cutaneous exposure (indicative limit value)
40 ppm TWA; 70 mg/m3 TWA
Skin notation
40 ppm TWA; 70 mg/m3 TWA
Potential for cutaneous absorption
40 ppm TWA; 70 mg/m3 TWA
Potential for cutaneous absorption
40 ppm TWA [VLA-ED] (indicative limit value); 68 mg/m3 TWA [VLA-ED] (indicative____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

Sweden:United Kingdom:SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedlimit value)
skin - potential for cutaneous exposure
30 ppm LLV; 50 mg/m3 LLV
60 ppm STV; 100 mg/m3 STV
40 ppm TWA; 68 mg/m3 TWA
60 ppm STEL; 102 mg/m3 STEL

Component Biological Limit Values
There are no biological limit values for the component(s) of this product.
Derived No Effect Levels (DNELs)
No DNELs available.
Predicted No Effect Concentrations (PNECs)
No PNECs available.

8.2 Exposure Controls
Appropriate Engineering Controls
Ventilation equipment should be explosion-resistant if explosive concentrations of material are present. Provide
local exhaust or process enclosure ventilation system. Ensure compliance with applicable exposure limits.
Personal Protective Equipment
Eye / Face Protection
Wear splash resistant safety goggles with a faceshield. (EN 166) Provide an emergency eye wash fountain andquick drench shower in the immediate work area.
Skin Protection
Wear appropriate chemical resistant clothing. (EN 340)
Glove Recommendations
Wear appropriate chemical resistant gloves. (EN 374)
Respiratory Protection
If airborne contaminant levels exceed recommended exposure limits, use CEN/EN Standard applicable
respiratory protection appropriate for employee exposure levels.
Consult with a health and safety professional for specific respirators appropriate for your use.
Environmental Exposure Controls
Avoid release to the environment.

* * *Section 9 - PHYSICAL AND CHEMICAL PROPERTIES* * *9.1 Information on Basic Physical and Chemical Properties
Physical State:
Color:
Odor:
pH:
Boiling Point:
Decomposition
Temperature:
LEL:
Vapor Pressure:
Density:
Water Solubility:
Coeff. Water/Oil Dist:

Liquidcolorless, transparent
odorless
Not available
82 °C (Acetonitrile)
Not available
Not available
Not available
Not available
Not available
Not available

Appearance:
Physical Form:
Odor Threshold:
Melting Point:
Flash Point:
Evaporation Rate:
UEL:
Vapor Density (air = 1):
Specific Gravity (water = 1):
Log KOW:
Auto Ignition Temperature:colorless, transparent liquid
liquidNot available
Not available
6 °C (COC) (Acetonitrile)
Not available
Not available
Not available
Not available
Not available
Not available____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

Viscosity:
Oxidizing Properties:
Flammability (solid, gas):

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedNot available
Not available
Not applicable

Volatility:
Explosive Properties:

Not available
Not available

9.2 Other Information
No additional information is available.

* * *Section 10 - STABILITY AND REACTIVITY* * *10.1 Reactivity
No reactivity hazard is expected.
10.2 Chemical Stability
Stable under normal conditions of use.
10.3 Possibility of Hazardous Reactions
Will not polymerize.
10.4 Conditions to Avoid
Avoid flames, sparks, and other sources of ignition. Containers may rupture or explode if exposed to heat. Avoid
contact with incompatible materials.
10.5 Incompatible Materials
acids, metals, bases, oxidizing materials, combustible materials, reducing agents
10.6 Hazardous Decomposition Products
Thermal Decomposition Products
Combustion: oxides of carbon, oxides of nitrogen, cyanides, hydrogen chloride gas* * *Section 11 - TOXICOLOGICAL INFORMATION* * *11.1 Information on Toxicological Effects
Acute and Chronic Toxicity
Component Analysis - LD50/LC50
The component(s) of this material have been reviewed in various sources and the following selected endpoints
are published:
Acetonitrile (75-05-8)
Dermal LD50 Rabbit 390 mg/kg; Inhalation LC50 Rat 26.8 mg/L 4 h (mist); Oral LD50 Rat 160 mg/kg
Water (7732-18-5)
Oral LD50 Rat >90 mL/kg
Irritation / Corrosivity
eye irritation
Respiratory Sensitisation
No information available for the product.
Skin Sensitisation
No information available for the product.
Germ Cell Mutagenicity
Available data characterizes component(s) of this product as a germ cell mutagenic hazard.
Carcinogenicity
Component Carcinogenicity
3,3'-Diaminobenzidine tetrahydrochloride (7411-49-6)
DFG:

Category 3B (could be carcinogenic for man)____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedReproductive Toxicity
No information available for the product.
Specific Target Organ Toxicity - Single Exposure
No target organs identified.
Specific Target Organ Toxicity - Repeated Exposure
No target organs identified.
Aspiration Hazard
Not available
Additional Data
No additional information is available.

* * *Section 12 - ECOLOGICAL INFORMATION* * *12.1 Toxicity
Component Analysis - Aquatic Toxicity
Acetonitrile (75-05-8)
Fish:

96 Hr LC50 Pimephales promelas: 1600 - 1690 mg/L [flow-through]; 96 Hr LC50 Pimephales
promelas: 1000 mg/L [static]; 96 Hr LC50 Lepomis macrochirus: 1850 mg/L [static]; 96 Hr LC50
Poecilia reticulata: 1650 mg/L [static]

12.2 Persistence and Degradability
No information available for the product.
12.3 Bioaccumulative Potential
No information available for the product.
12.4 Mobility in Soil
No information available for the product.
12.5 Results of PBT and vPvB Assessment
No information available.
EU - Interim Strategy for Management of PBT and vPvB Substances (PBT Assessments)
No components of this material are listed.
12.6 Other Adverse Effects
No additional information is available.

* * *Section 13 - DISPOSAL CONSIDERATIONS* * *13.1 Waste Treatment Methods
13.1.1 Product/Packaging Disposal
For disposal within the EC, use the appropriate code according to European Waste Catalogue (EWC).
13.1.2 Waste Treatment Relevant Information
Dispose in accordance with all applicable regulations.
13.1.3 Sewage Disposal Relevant Information
Prevent entry into sewers, drains, ditches, underground or confined spaces and waterways.
13.1.4 Other Disposal Recommendations
Dispose in accordance with all applicable regulations.

* * *Section 14 - TRANSPORT INFORMATION* * *14.1

UN Number

ADR
1648

RID
1648

ICAO
1648

ADN
1648

IMDG
1648

____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

14.2
14.3

14.4

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedUN Proper Shipping
Name
Transport Hazard
Class(es)Acetonitrile
MixtureHazardClass: 3

Acetonitrile
MixtureHazardClass: 3

Acetonitrile
MixtureHazardClass: 3

Acetonitrile
MixtureHazardClass: 3

Acetonitrile
MixtureHazardClass: 3

Packing Group

Packing
Group: II--

Packing
Group: II--

Packing
Group: II--

Packing
Group: II--

Packing
Group: II
--

--

--

--

--

--

--

--

--

Environmental
Hazards
Special Precautions-14.6
For User
Transport in Bulk
-14.7
According to Annex II
or MARPOL 73/78 andthe IBC CodeTransport/Additional information:
ADR
Limited quantities (LQ)
Excepted quantities (EQ)
14.5

Transport category
Tunnel restriction code

1L
Code: E2
Maximum net quantity per inner packaging: 30 ml
Maximum net quantity per outer packaging: 500 ml
2
D/E

IMDG
Limited quantities (LQ)
Excepted quantities (EQ)

1L
Code: E2
Maximum net quantity per inner packaging: 30 ml
Maximum net quantity per outer packaging: 500 ml

International Bulk Chemical CodeThis material contains one or more of the following chemicals required by the IBC Code to be identified as
dangerous chemicals in bulk.
Acetonitrile (75-05-8)
IBC Code:

Category Z; Category Y (low purity grade)* * *Section 15 - REGULATORY INFORMATION* * *15.1 Safety, Health and Environmental Regulations / Legislation Specific for the Substance or Mixture
EU - REACH (1907/2006) - Annex XIV List of Substances Subject to Authorisation
No components of this material are listed.
EU - REACH (1907/2006) - Article 59(1) Candidate List of Substances for Eventual Inclusion in Annex XIV
No components of this material are listed.
EU - REACH (1907/2006) - Annex XVII Restrictions of Certain Dangerous Substances, Mixtures and Articles____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedNo components of this material are listed.
Germany Regulations
Germany Water Classification
Acetonitrile (75-05-8)
ID Number 8, hazard class 2 - hazard to waters
Germany - Ordinance on Prohibition of Certain Chemicals
acetonitrile
3,3'-DIAMINOBENZIDINE TETRAHYDROCHLORIDE
Denmark Regulations
Environmental Protection Agency List of Undesirable Substances
No components of this material are listed.
Threshold of Exposure Concentrations of VOCs
No components of this material are listed.
France Water Pollution
No components of this material are listed.
Inventory
Substance Analysis - Inventory
Component
Acetonitrile
Water
3,3'-Diaminobenzidine tetrahydrochlorideCAS
75-05-87732-18-5
7411-49-6

EEC
EIN
EIN
EIN

15.2 Chemical Safety Assessment
No chemical safety assessment has been carried out for the substance/mixture.

* * *Section 16 - OTHER INFORMATION* * *
16.1
16.2

16.3
16.4
16.5

Indication of changes
New SDS: 18 April 2016
Key / Legend to abbreviations and acronyms used in the safety data sheet
ACGIH - American Conference of Governmental Industrial Hygienists; ADR - European Road Transport; CAS Chemical Abstracts Service; CLP - Classification, Labelling and Packaging; EEC - European Economic
Community; EIN (EINECS) - European Inventory of Existing Commercial Chemical Substances; ELN (ELINCS) European List of Notified Chemical Substances; IARC - International Agency for Research on Cancer; IATA International Air Transport Association; ICAO - International Civil Aviation Organization; IMDG - International
Maritime Dangerous Goods; IBC Code - International Bulk Chemical Code; Kow - Octanol/water partition
coefficient; LC50 - Lethal Concentration, 50%; LD50 - Lethal Dose, 50%; LEL - Lower Explosive Limit; LOLI - List
Of LIsts™ - ChemADVISOR's Regulatory Database; MAK - Maximum Concentration Value in the Workplace;
MEL - Maximum Exposure Limits; NTP = National Toxicology Program; REACH - Registration, Evaluation,
Authorisation and Restriction of Chemicals; RID - European Rail Transport; STEL - Short-term Exposure Limit;
TWA - Time Weighted Average; UEL - Upper Explosive Limit
Key literature references and sources for data
Available upon request
Methods used for classification of mixture according to Regulation (EC) No 1272/2008
Available upon request
Full text of H- phrases in Section 3____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent A

SDS ID: F-1000
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended

H225 Highly flammable liquid and vapour.
H302 Harmful if swallowed.
H312 Harmful in contact with skin.
H319 Causes serious eye irritation.
H332 Harmful if inhaled.
H341 Suspected of causing genetic defects.
H350 May cause cancer.
16.6 Training Advice
Read the Safety Data Sheet before handling product.
16.7

Other Information
The information set forth in this Safety Data Sheet does not purport to be all-inclusive and should be used only as
a guide. While the information and recommendations set forth herein are believed to be accurate, the company
makes no warranty regarding such information and recommendations and disclaims all liability from reliance
thereon.

End of Sheet F-1000____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent B

SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended* * *Section 1 - IDENTIFICATION OF THE SUBSTANCE / MIXTURE AND OF THE COMPANY /UNDERTAKING* * *1.1 Product Identifier:Product Identifier: DAB Reagent B
Substance Registration Number(s)This product and its components are not subject to REACH.
1.2 Relevant Identified Uses of the Substance or Mixture and Uses Advised Against
Identified Uses
immunohistochemical use
Reagent type: Bottle
425312F: -Histofine DAB-2V; DAB reagent B; 30.0 ml x 2425314F: -Histofine DAB-2V; DAB reagent B; 60.0 ml x 6
Uses Advised Against
Use in accordance with supplier's recommendations.
1.3 Details of the supplier of the safety data sheet
Nichirei Biosciences Inc.
6-19-20, Tsukiji, Chuo-Ku
Tokyo, Japan

Telephone number: +81-3-3248-2207
Email: n1060x005@nichirei.co.jp

1.4 Emergency Telephone Number+81-3-3248-2207 (9:00-17:00 Japan time)* * *Section 2 - HAZARDS IDENTIFICATION* * *2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No 1272/2008
No classification is assigned, based on classification criteria. Review the entire data sheet for any additional
information which did not result in a GHS classification.
2.2 Label Elements
Labelling according to Regulation (EC) No 1272/2008:
Symbol(s)None needed according to classification criteria.
Signal Word
None needed according to classification criteria.
Hazard Statement(s)None needed according to classification criteria.
Precautionary Statement(s)Prevention
None needed according to classification criteria.
Response
None needed according to classification criteria.
Storage
None needed according to classification criteria.
Disposal
Dispose in accordance with all applicable regulations.

____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent B

SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended

2.3 Other Hazards
No additional information is available.

* * *Section 3 - COMPOSITION / INFORMATION ON INGREDIENTS* * *
CAS
EC No
Registration No
Not Available--7722-84-1
231-765-0--

Component
Synonyms

1272/2008(CLP)

Percent

Aqueous salt solution

--

99.4

Hydrogen peroxide

Ox. Liq. 1
H271
Acute Tox. 4(Oral) H302
Acute Inh. Tox.
4 H332Skin Corr. 1A
H314
Note(s): B

0.6* * *Section 4 - FIRST AID MEASURES* * *4.1 Description of First Aid Measures
Inhalation
If adverse effects occur, remove to uncontaminated area. If breathing is difficult, oxygen should be administeredby qualified personnel. Get medical attention.
Skin
Wash with plenty of soap and water. If skin irritation occurs: Get medical advice/attention.
Eyes
Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue
rinsing. Get medical attention if irritation develops or persists.
Ingestion
If swallowed, get medical attention.
4.2 Most Important Symptoms and Effects, both Acute and Delayed
Acute
No information on significant adverse effects.
DelayedNo information on significant adverse effects.
4.3 Indication of any Immediate Medical Attention and Special Treatment Needed
Treat symptomatically and supportively.

* * *Section 5 - FIRE FIGHTING MEASURES* * *5.1 Extinguishing MediaUse extinguishing agents appropriate for surrounding fire.
Unsuitable Extinguishing MediaDo not use high-pressure water streams.

____________________________________________________________
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Revision 2.0000

Print Date: 18/04/2016

Safety Data Sheet
Material Name: DAB Reagent B

SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended5.2 Special Hazards Arising from the Substance or Mixture
Negligible fire hazard.
Thermal Decomposition Products
Combustion: miscellaneous decomposition products
5.3 Advice for Firefighters
Fire Fighting Measures
Move container from fire area if it can be done without risk. Cool containers with water spray until well after thefire is out.
Protective Equipment and Precautions for Firefighters
Wear personal protective clothing and equipment such as self-contained breathing apparatus (SCBA) for
protection against possible exposure.

* * *Section 6 - ACCIDENTAL RELEASE MEASURES* * *6.1 Personal Precautions, Protective Equipment and Emergency Procedures
Wear personal protective clothing and equipment, see Section 8.
6.2 Environmental Precautions
Avoid release to the environment.
6.3 Methods and Material for Containment and Cleaning up
Absorb with sand or other non-combustible material. Collect spilled material in appropriate container for disposal.
Keep unnecessary people away, isolate hazard area and deny entry.
6.4 Reference to Other Sections
See Section 7 for Handling Procedures. See Section 8 for Personal Protective Equipment recommendations. See
Section 13 for Disposal Considerations.

* * *Section 7 - HANDLING AND STORAGE* * *7.1 Precautions for Safe Handling
Avoid breathing vapor or mist. Avoid contact with skin and eyes. Wash thoroughly after handling.
7.2 Conditions for Safe Storage, Including any Incompatibilities
Store and handle in accordance with all current regulations and standards.
7.3 Specific End Use(s)immunohistochemical use* * *Section 8 - EXPOSURE CONTROLS / PERSONAL PROTECTION* * *8.1 Control Parameters
Component Exposure Limits
Hydrogen peroxide (7722-84-1)
Austria:
Belgium:
Bulgaria:
Croatia:
Denmark:
Estonia:
Finland:

1 ppm TWA [TMW]; 1.4 mg/m3 TWA [TMW]
2 ppm STEL [KZW]; 2.8 mg/m3 STEL [KZW]
1 ppm TWA; 1.4 mg/m3 TWA
1.5 mg/m3 TWA
1 ppm TWA [GVI]; 1.4 mg/m3 TWA [GVI]
2 ppm STEL [KGVI]; 2.8 mg/m3 STEL [KGVI]
1 ppm TWA; 1.4 mg/m3 TWA
1 ppm TWA; 1.4 mg/m3 TWA
2 ppm Ceiling; 3 mg/m3 Ceiling
1 ppm TWA (also solutions); 1.4 mg/m3 TWA (also solutions)3 ppm STEL; 4.2 mg/m3 STEL____________________________________________________________
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Print Date: 18/04/2016

Safety Data Sheet
Material Name: DAB Reagent B

France:Germany (DFG):
Greece:Ireland:Lithuania:
Poland:Portugal:
Slovak Republic:
Slovenia:
Spain:
Sweden:United Kingdom:SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended

1 ppm TWA [VME]; 1.5 mg/m3 TWA [VME]
0.5 ppm TWA MAK; 0.71 mg/m3 TWA MAK
0.5 ppm Peak; 0.71 mg/m3 Peak
1 ppm TWA; 1.4 mg/m3 TWA
3 mg/m3 STEL
1 ppm TWA; 1.5 mg/m3 TWA
2 ppm STEL; 3 mg/m3 STEL
1 ppm TWA [IPRD]; 1.4 mg/m3 TWA [IPRD]
2 ppm Ceiling [NRD]; 3 mg/m3 Ceiling [NRD]
0.4 mg/m3 TWA [NDS]
0.8 mg/m3 STEL [NDSCh]
1 ppm TWA [VLE-MP]
1 ppm TWA; 1.4 mg/m3 TWA
2.8 mg/m3 Ceiling
1 ppm TWA; 1.4 mg/m3 TWA
1 ppm STEL; 1.4 mg/m3 STEL
1 ppm TWA [VLA-ED]; 1.4 mg/m3 TWA [VLA-ED]
1 ppm LLV; 1.4 mg/m3 LLV
2 ppm CLV; 3 mg/m3 CLV
1 ppm TWA; 1.4 mg/m3 TWA
2 ppm STEL; 2.8 mg/m3 STEL

Component Biological Limit Values
There are no biological limit values for the component(s) of this product.
Derived No Effect Levels (DNELs)
No DNELs available.
Predicted No Effect Concentrations (PNECs)
No PNECs available.

8.2 Exposure Controls
Appropriate Engineering Controls
Provide local exhaust ventilation system. Ensure compliance with applicable exposure limits.
Personal Protective Equipment
Eye / Face Protection
Wear splash resistant safety glasses. (EN 166) Provide an emergency eye wash fountain and quick drench
shower in the immediate work area.
Skin Protection
Wear appropriate chemical resistant clothing. (EN 340)
Glove Recommendations
Wear appropriate chemical resistant gloves. (EN 374)
Respiratory Protection
If airborne contaminant levels exceed recommended exposure limits, use CEN/EN Standard applicable
respiratory protection appropriate for employee exposure levels.
Consult with a health and safety professional for specific respirators appropriate for your use.
Environmental Exposure Controls
Avoid release to the environment.

____________________________________________________________
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Print Date: 18/04/2016

Safety Data Sheet
Material Name: DAB Reagent B

SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended* * *Section 9 - PHYSICAL AND CHEMICAL PROPERTIES* * *9.1 Information on Basic Physical and Chemical Properties
Physical State:
Color:
Odor:
pH:
Boiling Point:
Decomposition
Temperature:
LEL:
Vapor Pressure:
Density:
Water Solubility:
Coeff. Water/Oil Dist:
Viscosity:
Explosive Properties:

Liquidcolorlessodorless
Not available
Not available
Not available

Appearance:
Physical Form:
Odor Threshold:
Melting Point:
Flash Point:
Evaporation Rate:colorless liquid
liquidNot available
Not available
Not available
Not available

Not available
Not available
Not available
Not available
Not available
Not available
Not available

UEL:
Vapor Density (air = 1):
Specific Gravity (water = 1):
Log KOW:
Auto Ignition Temperature:
Oxidizing Properties:
Flammability (solid, gas):

Not available
Not available
Not available
Not available
Not available
Not available
Not applicable

9.2 Other Information
No additional information is available.

* * *Section 10 - STABILITY AND REACTIVITY* * *10.1 Reactivity
No reactivity hazard is expected.
10.2 Chemical Stability
Stable at standard temperatures and pressure.
10.3 Possibility of Hazardous Reactions
Will not polymerize.
10.4 Conditions to Avoid
None known.
10.5 Incompatible Materials
None known.
10.6 Hazardous Decomposition Products
Thermal Decomposition Products
Combustion: miscellaneous decomposition products* * *Section 11 - TOXICOLOGICAL INFORMATION* * *11.1 Information on Toxicological Effects
Acute and Chronic Toxicity
Component Analysis - LD50/LC50
The component(s) of this material have been reviewed in various sources and the following selected endpoints
are published:
Hydrogen peroxide (7722-84-1)
Oral LD50 Rat 1518 mg/kg; Dermal LD50 Rabbit 2000 mg/kg; Dermal LD50 Rat 4060 mg/kg; Inhalation LC50 Rat
2 g/m3 4 h
Irritation / Corrosivity
May cause irritation.

____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent B

SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedRespiratory Sensitisation
No information available for the product.
Skin Sensitisation
No information available for the product.
Germ Cell Mutagenicity
No information available for the product.
Carcinogenicity
Component Carcinogenicity
Hydrogen peroxide (7722-84-1)
IARC:
DFG:

Monograph 71 [1999]; Supplement 7 [1987]; Monograph 36 [1985] (Group 3 (not classifiable))
Category 4 (no significant contribution to human cancer)

Reproductive Toxicity
No information available for the product.
Specific Target Organ Toxicity - Single Exposure
No target organs identified.
Specific Target Organ Toxicity - Repeated Exposure
No target organs identified.
Aspiration Hazard
No information available for the product.

* * *Section 12 - ECOLOGICAL INFORMATION* * *12.1 Toxicity
Component Analysis - Aquatic Toxicity
Hydrogen peroxide (7722-84-1)
Fish:Invertebrate:

96 Hr LC50 Pimephales promelas: 16.4 mg/L; 96 Hr LC50 Lepomis macrochirus: 18 56 mg/L [static]; 96 Hr LC50 Oncorhynchus mykiss: 10.0 - 32.0 mg/L [static]
48 Hr EC50 Daphnia magna: 18 - 32 mg/L [Static]

12.2 Persistence and Degradability
No information available for the product.
12.3 Bioaccumulative Potential
No information available for the product.
12.4 Mobility in Soil
No information available for the product.
12.5 Results of PBT and vPvB Assessment
No information available.
EU - Interim Strategy for Management of PBT and vPvB Substances (PBT Assessments)
No components of this material are listed.
12.6 Other Adverse Effects
No additional information is available.

* * *Section 13 - DISPOSAL CONSIDERATIONS* * *13.1 Waste Treatment Methods
13.1.1 Product/Packaging Disposal
For disposal within the EC, use the appropriate code according to European Waste Catalogue (EWC).
13.1.2 Waste Treatment Relevant Information
Dispose in accordance with all applicable regulations.

____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent B

SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amended13.1.3 Sewage Disposal Relevant Information
Prevent entry into sewers, drains, ditches, underground or confined spaces and waterways.
13.1.4 Other Disposal Recommendations
Dispose in accordance with all applicable regulations.

* * *Section 14 - TRANSPORT INFORMATION* * *Transportation
Not regulated as dangerous goods
International Bulk Chemical CodeThis material contains one or more of the following chemicals required by the IBC Code to be identified as
dangerous chemicals in bulk.
Hydrogen peroxide (7722-84-1)
IBC Code:

Category Y (solutions, >60% but not >70% by mass); Category Y (solutions, >8% but not >60%
by mass)* * *Section 15 - REGULATORY INFORMATION* * *15.1 Safety, Health and Environmental Regulations / Legislation Specific for the Substance or Mixture
EU - REACH (1907/2006) - Annex XIV List of Substances Subject to Authorisation
No components of this material are listed.
EU - REACH (1907/2006) - Article 59(1) Candidate List of Substances for Eventual Inclusion in Annex XIV
No components of this material are listed.
EU - REACH (1907/2006) - Annex XVII Restrictions of Certain Dangerous Substances, Mixtures and Articles
No components of this material are listed.
Germany Regulations
Germany Water Classification
Hydrogen peroxide (7722-84-1)
ID Number 288, hazard class 1 - low hazard to waters (footnote 8)
Denmark Regulations
Environmental Protection Agency List of Undesirable Substances
No components of this material are listed.
Threshold of Exposure Concentrations of VOCs
No components of this material are listed.
France Water Pollution
No components of this material are listed.
Inventory
Substance Analysis - Inventory
Component
Hydrogen peroxide

CAS7722-84-1

EEC
EIN

15.2 Chemical Safety Assessment
No chemical safety assessment has been carried out for the substance/mixture.

* * *Section 16 - OTHER INFORMATION* * *
16.1
16.2

Indication of changes
New SDS: 18 April 2016
Key / Legend to abbreviations and acronyms used in the safety data sheet____________________________________________________________
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Safety Data Sheet
Material Name: DAB Reagent B

16.3
16.4
16.5

16.6
16.7

SDS ID: F-1003
In compliance with Regulation(EC) 1907/2006 (REACH) as
amendedACGIH - American Conference of Governmental Industrial Hygienists; ADR - European Road Transport; AIHA American Industrial Hygiene Association; CAS - Chemical Abstracts Service; CERCLA - Comprehensive
Environmental Response, Compensation, and Liability Act; CLP - Classification, Labelling and Packaging; DNEL Derived No Effect Level; DOT - Department of Transportation; DSD - Dangerous Substances Directive(67/548/EEC); DSL - Domestic Substances List; EC50 - Effective Concentration, 50%; EEC - European Economic
Community; EIN (EINECS) - European Inventory of Existing Commercial Chemical Substances; ELN (ELINCS) European List of Notified Chemical Substances; EPA - Environmental Protection Agency; IARC - International
Agency for Research on Cancer; IATA - International Air Transport Association; IBC Code - International Bulk
Chemical Code; IMDG - International Maritime Dangerous Goods; Kow - Octanol/water partition coefficient; LC50
- Lethal Concentration, 50%; LD50 - Lethal Dose, 50%; LEL - Lower Explosive Limit; NFPA - National Fire
Protection Agency; NIOSH - National Institute for Occupational Safety and Health; NTP - National Toxicology
Program; OSHA - Occupational Safety and Health Administration; PNEC - Predicted No Effect Concentration;
REACH - Registration, Evaluation, Authorisation and Restriction of Chemicals; RID - European Rail Transport;
SARA - Superfund Amendments and Reauthorization Act; STEL - Short-term Exposure Limit; TDG Transportation of Dangerous Goods; TSCA - Toxic Substances Control Act; TWA - Time Weighted Average; UEL- Upper Explosive Limit; WHMIS - Workplace Hazardous Materials Information System
Key literature references and sources for data
Available upon request
Methods used for classification of mixture according to Regulation (EC) No 1272/2008
Available upon request
Full text of H- phrases in Section 3
H271 May cause fire or explosion; strong oxidizer.
H302 Harmful if swallowed.
H314 Causes severe skin burns and eye damage.
H332 Harmful if inhaled.
Training Advice
Read the Safety Data Sheet before handling product.
Other Information
Disclaimer: Supplier gives no warranty whatsoever, including the warranties of merchantability or of fitness for aparticular purpose. Any product purchased is sold on the assumption the purchaser shall determine the quality
and suitability of the product. Supplier expressly disclaims any and all liability for incidental, consequential or any
other damages arising out of the use or misuse of this product. No information provided shall be deemed to be arecommendation to use any product in conflict with any existing patent rights.

End of Sheet F-1003____________________________________________________________
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