-Histofine® ALK Detection Kit

Material Safety Data Sheet
1. PRODUCT IDENTIFICATIONCREATION DATE: 8/Dec./2010
Product Name :-Histofine® ALK Detection Kit
Product Number : 417071F(20tests)
REVISION DATE: 10/Feb/2012 *Consistent Name :PRODUCT USE: research
6. Chromogen Substrate
1. Blocking Reagent
7. Substrate Buffer Solution
2. Primary Antibody
8. Chromogen Reagent
3. Negative Control
9. ALK Antigen Retrieval Solution A
4. Bridge Reagent
10.ALK Antigen Retrieval Solution B
5. Peroxidase labeled Empower Reagent
Manufacture's Name:
Emergency Telephone Number
NICHIREI BIOSCIENCES INC.
81-3-3248-2207
Address (Number, Street, City, State, and ZIP Code):
6-19-20, TSUKIJI, CHUO-KU, TOKYO, 104-8402, JAPAN2. COMPOSITION and INFORMATION ON INGREDIENTS
1. Blocking Reagent, 8. Chromogen Reagent
1 COMPONENT: Hydrogen peroxide
CAS NUMBER: 7722-84-1
EC NUMBER (EINECS): 231-765-0
PERCENTAGE: <10EC CLASSIFICATION: No classification assigned.
2 COMPONENT: Non-hazardous
PERCENTAGE: 90-100
2. Primary Antibody, 3. Negative Control, 4. Bridge Reagent
and 9. ALK Antigen Retrieval Solution A
1 COMPONENT: Sodium azideCAS NUMBER:
NUMBER: 26628-22-8
EC NUMBER (EINECS): 247-852-1
PERCENTAGE: <0.1
EC CLASSIFICATION: No classification assigned.
2 COMPONENT: Non-hazardous
PERCENTAGE: 99-100

5. Peroxidase labeled Empower Reagent
and 7. Substrate Buffer Solution
1 COMPONENT: Non-hazardous
PERCENTAGE: 100
6. Chromogen Substrate
1 COMPONENT: 3,3'-Diaminobenzidine tetrahydrochloride
CAS NUMBER: 7411-49-6
EC NUMBER (EINECS): 231-018-9
PERCENTAGE: <1
EC CLASSIFICATION: No classification assigned.
2 COMPONENT: Non-hazardous
PERCENTAGE: 90-100
10. ALK Antigen Retrieval Solution B
1 COMPONENT: Ethylene glycol
CAS NUMBER: 107-21-1
EC NUMBER (EINECS): 203-473-3
PERCENTAGE: <40
EC CLASSIFICATION: No classification assigned.
2 COMPONENT: Non-hazardous
PERCENTAGE: 60-100

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3. HAZARDS IDENTIFICATION
1. Blocking Reagent, 8. Chromogen Reagent
EU LABELING AND CLASSIFICATION: This product meets the definition of the following hazard class as defined by
the European Economic Community Guidelines.
EU CLASSIFICATION: [O] OXIDIZER, [Xn] HARMFUL. [C] CORROSIVEEU RISK PHRASES:
R 8: Contact with combustible material may cause fire; R22: Harmful if swallowed; R34: Causes burns;
R41: Risk of serious damage to eyes.
EU SAFETY PHRASES: S3: Keep in cool place; S28: After contact with skin, wash immediately with plenty of water;
S37: Wear suitable gloves; S39: Wear eye/face protection; S45: In case of accident or if you feel unwell, seek
medical advice immediately.

EMERGENCY OVERVIEW:
COLOUR: Colorless except 2.: green, 4.: reddish violet, 5.: light yellow, 6.: pink-Violet, and 10.:light yellow
PHYSICAL FORM: liquid
ODOUR: odourless eｘcept 1., 8.：Slight acrid odor
MAJOR HEALTH HAZARDS: No significant target effects reported.
POTENTIAL HEALTH EFFECTS:
INHALATION:SHORT TERM EXPOSURE: irritation
LONG TERM EXPOSURE: no information on significant adverse effects
SKIN CONTACT:SHORT TERM EXPOSURE: irritation
LONG TERM EXPOSURE: irritation
EYE CONTACT:SHORT TERM EXPOSURE: irritation
LONG TERM EXPOSURE: no information on significant adverse effects
INGESTION:SHORT TERM EXPOSURE: nausea, vomiting,LONG TERM EXPOSURE: no information on significant adverse effects
diarrhoea
4. FIRST AID MEASURESINHALATION: If adverse effects occur, remove to uncontaminated area. Give artificial respiration if not breathing. Get
immediate medical attention.
SKIN CONTACT: Wash contaminated areas with soap and water. Thoroughly clean and dry contaminated clothing before
reuse. Get medical attention, if needed.
EYE CONTACT: Flush eyes with plenty of water for at least 15 minutes. Then get

INGESTION: If a large amount is swallowed, get medical attention.
5. FIRE FIGHTING MEASURES
FIRE AND EXPLOSION HAZARDS: Negligible fire hazard.
EXTINGUISHING MEDIA: Use extinguishing agents appropriate for surrounding fire.
FIRE FIGHTING: Move container from fire area if it can be done without risk. Avoid inhalation of material or combustion by-products.
SENSITIVITY TO MECHANICAL IMPACT: Not sensitive
SENSITIVITY TO STATIC DISCHARGE: Not sensitive
FLASH POINT: not flammable
HAZARDOUS COMBUSTION PRODUCTS: Thermal decomposition products or combustion: oxides of carbon
6. ACCIDENTAL RELEASE MEASURESOCCUPATIONAL RELEASE:Absorb with sand or other non-combustible material. Collect spilled material in appropriate
container for disposal.
7. HANDLING AND STORAGE
STORAGE: Store and handle in accordance with all current regulations and standards. Store between 2 C and 8 C. See
original container for storage recommendations. Keep separated from incompatible substances.

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8. EXPOSURE CONTROLS/PERSONAL PROTECTIONEXPOSURE LIMITS:
VENTILATION: Provide local exhaust ventilation system. Ensure compliance with applicable exposure limits.
EYE PROTECTION: Wear splash resistant safety goggles. Provide an emergency eye
wash fountain and quick drench shower in the immediate work area.
CLOTHING: Lab coat or apron.
GLOVES: Wear appropriate chemical resistant gloves.
PROTECTIVE MATERIAL TYPES: latex, vinyl
RESPIRATOR: No respirator is required under normal conditions of use.
9. PHYSICAL AND CHEMICAL PROPERTIES
1. Blocking Reagent, 8. Chromogen Reagent
VAPOR DENSITY: Not Available Air10
SPECIFIC GRAVITY @ 20C: 1.11 water1
SOLUBILITY IN WATER: 100%
VAPOR PRESSURE, mm Hg @ 20°C 68F: 24
ODOR: Slight acrid odor
APPEARANCE and COLOR: Clear colorless Liquid

EVAPORATION RATE n-BuAc1: No Data
MELTING POINT: -26ºC
BOILING POINT: 107º C
pH: -6.0

2. Primary Antibody, 3. Negative Control, 4. Bridge Reagent and 9. ALK Antigen Retrieval Solution A
PHYSICAL STATE: liquid
SPECIFIC GRAVITY: Not available
ODOUR: odourless
WATER SOLUBILITY: miscible
BOILING POINT: Not available
VOLATILITY: Not available
FREEZING POINT: Not available
ODOUR THRESHOLD: Not available
VAPOUR PRESSURE: Not available
EVAPORATION RATE: Not available
VAPOUR DENSITY: Not available
COEFFICIENT OF WATER/OIL DISTRIBUTION: Not available
10. STABILITY AND REACTIVITY

REACTIVITY: Stable at normal temperatures and pressure.
CONDITIONS TO AVOID: None reported.
INCOMPATIBILITIES: oxidising materials, strong acids
HAZARDOUS DECOMPOSITION:
Thermal decomposition products or combustion: oxides of carbon
POLYMERISATION: Will not polymerise.
11. TOXICOLOGICAL INFORMATION

Sodium azide:
ACUTE TOXICITY LEVEL: Insufficient data.
ADDITIONAL DATA: none
Hydrogen peroxide
ACUTE TOXICITY LEVEL: Insufficient data.
ADDITIONAL DATA: IARC has concluded that no adequate data on the carcinogenicity of hydrogenperoxide
are available to the Working Group.
3,3'-Diaminobenzidine tetrahydrochloride:
ACUTE TOXICITY LEVEL: Insufficient data.
ADDITIONAL DATA: IARC has concluded that Benzidine and its salts are carcinogenic. Although thissubstance is structurally related to Benzidine, there is no current evidence that it is a carcinogen.
Etylene Glycol
ACUTE TOXICITY LEVEL: Insufficient data.
ADDITIONAL DATA: none

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12. ECOLOGICAL INFORMATION

Not available
13. DISPOSAL CONSIDERATIONS

Dispose in accordance with all applicable regulations.
14. TRANSPORT INFORMATION

U.S. DEPARTMENT OF TRANSPORTATION: Not regulated.
CANADIAN TRANSPORTATION OF DANGEROUS GOODS: Not regulated.
LAND TRANSPORT ADR: Not regulated.
AIR TRANSPORT IATA: Not regulated.
LAND TRANSPORT RID: Not regulated.
AIR TRANSPORT ICAO: Not regulated.
MARITIME TRANSPORT IMDG: Not regulated.
15. REGULATORY INFORMATION

U.S. REGULATIONS:CERCLA SECTIONS 102a/103 HAZARDOUS SUBSTANCES (40 CFR 302.4): Not regulated.
SARA TITLE III SECTION 302 EXTREMELY HAZARDOUS SUBSTANCES (40 CFR 355.30): Not regulated.
SARA TITLE III SECTION 304 EXTREMELY HAZARDOUS SUBSTANCES (40 CFR 355.40): Not regulated.
SARA TITLE III SARA SECTIONS 311/312 HAZARDOUS CATEGORIES (40 CFR 370.21):
ACUTE: Yes
FIRE: NoCHRONIC: Yes
REACTIVE: No
SUDDEN RELEASE: No
SARA TITLE III SECTION 313 (40 CFR 372.65): Not regulated.
STATE REGULATIONS:
California Proposition 65: Not regulated.
CANADIAN REGULATIONS:WHMIS CLASSIFICATION: Not a Controlled Product under Canada's Workplace Hazardous Material
Information System.
EUROPEAN REGULATIONS:EC CLASSIFICATION (CALCULATED):No classification assigned.
16. OTHER INFORMATION *Limitations: The information and recommendations set forth in this SDS are believed to be correct as of this date.
Nichirei Biosciences, Inc. makes no warranty with respect to the content of this SDS and disclaims all liability fromreliance thereon.

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