Page 1 of 9Issuing date: January 6, 2015

Material Safety Data Sheet
The content and format of this SDS is accordant with GHS standard.

1. Identification of the substance/preparation and of the company/undertaking
Product name: - Histofine DAB-3S kit
Product Number: 415192F: 500 tests, 415194F: 1500 tests
Relevant identified uses of the substance or mixture and uses advised against: For immunohistochemical use.
Manufacturer/Supplier: NICHIREI BIOSCIENCES INC.
Address: 6-19-20, Tsukiji, Chuo-ku,Tokyo, 104-8402, Japan
Tel.: +81-3-3248-2207
Fax: +81-3-3248-2243
Further information obtainable from: NICHIREI BIOSCIENCES INC.
Information in case of emergency:
Tel.: +81-3-3248-2207

2. Hazards identification
GHS classification:
Physical hazards

Health hazards

Environmental hazards

Explosives-not classified
Flammable gases-not classified
Flammable aerosols-not classified
Gases under pressure- not classified
Flammable liquids- not classified
Flammable solids-not classified
Self-reactive substances and mixtures-notclassified
Pyrophoric liquids-not classified
Pyrophoric solids-not classified
Self-heating substances and mixtures-notclassified
Substances and mixtures, which incontact with water, emit flammablegases-not classified
Oxidizing liquids-not classified
Oxidizing solids-not classified
Organic peroxides-not classified
Corrosive to metals-not classified

Acute toxicity (oral)- not classified
Acute toxicity (dermal)-not classified
Acute toxicity (inhalation)- not classified
Skin corrosion/irritation-3
Serious eye damage/eye irritation-2B
Respiratory sensitizer-not classified
Skin sensitizer-not classified
Germ cell mutagenicity- not classified
Carcinogenicity-not classified
Toxic to reproduction-1B
Effects on or via lactation-not classified
Specific target organs/systemic toxicity
following single exposure -1 (Centralnervous system, kidney, heart andrespiratory organs)
Specific target organs/systemic toxicity
following repeated exposure-1 (Central nervous system, heart andrespiratory organs)-2 (Lung and blood)
Aspiration hazard- not classified

Acute hazards to the aquaticenvironment-3
Chronic hazards to the aquaticenvironment-not classified

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Issuing date: January 6, 2015

Signal word: Danger
Symbol :.Hazard Statement:
H316: Causes mild skin irritation.
H320: Causes eye irritation.
H360: May damage fertility or the unborn child.
H370: Causes damage to organs (Central nervous system, kidney, heart and respiratory organs).
H372: Causes damage to organs (Central nervous system, heart and respiratory organs) through prolonged or repeatedexposure.
H373: May cause damage to organs (Lung and blood) through prolonged or repeated exposure.
H402: Harmful to aquatic life.
Prevention Precautionary Statements:
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P260: Do not breathe dust/fume/gas/mist/vapour/spray.
P264: Wash hands thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P273: Avoid release to the environment.
P281: Use personal protective equipment as required.
Response Precautionary Statements:
P333+P313: If skin irritation or a rash occurs: Get medical advice/attention.
P305+P351+P338: IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy
to do-continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
P308+P313: IF exposed or concerned: Get medical advice/attention.
P321: Specific treatment (see specific information on this label).
P314: Get Medical advice/attention if you feel unwell.
Storage precautionary statements :P405: Store locked up.
Disposal precautionary statements :P501: Dispose of contents/container to relevant local and national regulations.

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Issuing date: January 6, 2015

3. Composition/information on ingredients
Product description: substance (); preparation/mixture (√)(1) DAB Reagent A
Ingredient (s)CAS#EC#Official Notice
Reference
Number (Japan)

3,3′-Diaminobenzidinehydrochloride7411-49-6

231-018-9--＜2.50%

Aqueous salt solution------＞97.50%

CAS#EC#Official Notice
ReferenceNumber (Japan)

7722-84-1

231-765-0--＜0.6%------＞99.40%% by weight(2) DAB Reagent B
Non-hazardous(3) DAB Reagent C
Ingredient (s)Hydrogen peroxide
Water% by weight

4 First aid measures
Persons using this product should consult a physician or other medical professional if an accident
involving this product results in injury. Specific first-aid measures are as follows:Eyes Contact: Remove contact lenses if present and easy to do, continue rinsing. If irritation persists, seek medical care.
Skin Contact: Wash with soap and water. If skin irritation or a rash occurs: Get medical advice/attention. Wash contaminatedclothing before reuse.
Inhalation: Remove victim to fresh air and keep at rest in a position comfortable for breathing. Call a POISON CENTER ordoctor/physician if you feel unwell.
Ingestion: Rinse mouth. Do not induce vomiting without professional instruction. Do not give unconscious persons anythingto eat. Call a POISON CENTER or doctor/physician if you feel unwell.
Acute effect and delayed effect: Acute effect: It causes irritation to eyes and mild irritation to skin. Inhaling or swallowing
this product can cause adverse effects on central nervous system, kidney, heart and respiratory organs.
Delayed effect: It causes damage to central nervous system, heart and respiratory organs through prolonged or repeatedexposure. It may cause damage to lung and blood through prolonged or repeated exposure.
Personal protective equipment : Wear protective gloves (e.g. rubber gloves) /protective clothing (clothing made of antistatic
synthetic fiber ) /eye protection/face protection.Wear respirators and safety goggles when exposure limits are exceeded orliquid may splash.
Indication of any immediate medical attention and special treatment needed: Treat symptoms as they occur.

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5. Fire-fighting measures
Extinguishing Media: This product is not combustible. As for fire caused by other ignition sources, choose appropriate
extinguishing media according to fire types. Recommend seeking advice from local brigade.
Unsuitable Extinguishing Media: Discharging cylinder shape water from fire hose may lead to spread fire to thesurroundings.
Special Fire-Fighting Method:
For initial fire, use dry powder, carbon dioxide, etc.
For large fire, it is effective to use fire foam, etc. to shut off air supply.
Firefighters must wear self-contained breathing apparatus and full protective equipment (e.g. fire-retardant clothing).
Deny unnecessary entry to the place around the fire.
Remove containers from fire area if it can be done without risk.
Cool surrounding facilities, etc. with water spray.
Extinguish fire from upwind, and the fire extinguishing method should be appropriate to the situation in the surroundings.
Fire Fighting Equipment: Firefighters must wear self-contained breathing apparatus and full protective equipment.
Unusual Fire and Explosion Hazards: It may present explosive danger if the container is heated.

6. Accidental release measures
Personal precautions: Use proper personal protective equipment as indicated in Section 8.
Measures for Cleaning/Collection:
Do NOT touch spilled material without any hand protection.
Stop leakage if it can be done without risk.
Mop up or absorb with an inert dry material (e.g. sand, dry lime) and place in an appropriately labeled waste disposalcontainer.
Environmental Precautions: Keep cleaning run-offs out of municipal sewers and open bodies of water. Comply with local
and national laws and regulations.

7. Handling and storage
Handling:
Do not handle until all safety precautions have been read and understood.
Workers must wear proper protective equipment and must operate strictly according to relative rules.
Do not breathe vapour or fume from processing.
Avoid damage of packaging or containers during handling.
Information about fire- and explosion protection:
As for this product, no special measures are required for fire and explosion protection. Considering good industrial practice,keep ignition sources away. Do not smoke in workplace.
Storage:
Requirements to be met by storerooms: Store it in a well-ventilated, cool and dry store room. Avoid long-time exposure todirect sunlight. Store locked up.
Additional Information: The storeroom should be equipped with proper facilities for accidental leakage and fire.

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Issuing date: January 6, 2015

8. Exposure controls/personal protection
Control parameters:
Ingredients

OSHA PEL-TWA

ACGIH TLV-TWA

Japan Industry &Health Association-PELs（2012-2013）

Hydrogen peroxide
CAS: 7722-84-1

1.4mg/m3

1.4mg/m3

No data available

Engineering Controls:
If user operations generate vapour or fume, use ventilation to keep exposure to airborne contaminants below the exposure
limit.
Personal Protective Equipment (for workers):
Protection of Hands:
Wear protective gloves. (e.g. impermeable rubber gloves)Protection of Eyes:
No special requirements under normal conditions. Wear safety glasses when liquid may contact eyes.

Respiratory Protection: Use an approved respirator if exposure limits are exceeded or if irritation or other symptoms occur.

Protection of Body:
Recommend wearing impermeable working clothing.

General protective and hygienic measures:
Do not breathe vapour or fume from processing.
Wash hands before breaks and at the end of work.
Avoid contact with eyes and skin.
Wash contaminated clothing before reuse.

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Issuing date: January 6, 2015

9. Physical and chemical properties
General Information
FormLiquidColour

DAB Reagent A: Pink to violet
The other reagents except above: Colorless and transparent

Odour

Odourless

pH Value

No data available

Boiling Range

No data available

Melting point/Melting range

No data available

Flash point

Not applicable

Flammable/Explosive Limits-Lower Vol %Not applicable

Flammable/Explosive L mits-Upper Vol %Not applicable

Density

No data available

Relative Vapour density

No data available

Vapour pressure

No data available

Solubility in/Miscibility

Soluble in water.

n-octanol/Water Partition Coefficient4.62(at 25℃)

Self-igniting

Not applicable

Decomposition Temperature

Not available

Odour Threshold Value

Not available

Evaporation Rate

Not available

Flammability (solid, gas, etc.)

This product is not classified as flammable liquid.

10. Stability and reactivity
Chemical Stability: Stable under normal temperatures and pressures.
Hazardous Reactions: Not found.
Materials to avoid: No special requirements.
Hazardous Decomposition Products: It may generate irritating and hazardous fume or vapour from thermal decomposition.
Conditions to Avoid: Long-time direct sunlight and high temperature.

11. Toxicological information
Product Toxicity Data: The toxicity data of this product has not been determined by testing, the toxicity data shown below isfor reference only.
Ingredients

CASLD 50 /LC 50 (Median lethal dose)

Hydrogen peroxide7722-84-1

Acute toxicity (Oral) LD50: 311 mg/kg (rat)Acute toxicity (Dermal) LD50: 4,060mg/kg (rat)Acute toxicity (Inhalation)LC50: 1438ppm (rat)Serious eye damage/eye irritation: This product causes eye irritation and is classified into Category 2B per GHS criteria.
Skin corrosion/irritation: This product causes mild irritation to skin and is classified into Category 3 per GHS criteria.

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Issuing date: January 6, 2015

Respiratory /Skin sensitizer: This product may cause an allergic skin reaction.
Germ cell mutagenicity: No classification for this product.
Carcinogenicity: No classification for this product.
Reproductive Toxicity: This product may damage fertility or the unborn child and classified into Category 1B per GHS
criteria.
STOT SE (Single exposure):
Category 1 (Central nervous system, kidney, heart and respiratory organs)
STOT RE (Repeated exposure):
Category 1 (Central nervous system, heart and respiratory organs);
Category 2 (Lung and blood)
Aspiration hazard: No classification for this product.

12. Ecological information
Ecotoxicity: No data available.
Persistence and Degradability: No data available.
Bioaccumulative Potential: No data available
Mobility in Soil: Due to its liquid status, it can move in soil.

13. Disposal considerations
Do not allow product to reach sewage system and any open bodies of water.
Any disposal practice must be in compliance with country, local, state, and federal laws and regulations.
Choose a professional and legal company for waste disposal.

14. Transport Information
DOT/ Air-Transportation- IATA/ICAO/Sea-Transportation-IMO/IMDG:
Proper Shipping Name: Not regulated.
Hazard Class: Not classified.
UN Code: Not regulated.
Packing Group: Not classified
Packing Group Symbol: Not classified.
Marine Pollutant (Yes/No): No
EMS NO.: Not regulated.
.Note:
Check whether the package is completed or sealed before transporting; make sure there are no damage and no falling down ofpackages during transporting; the transport vehicle should be equipped with fire-fighting equipment and emergency facilities
for handling with release; do NOT transport this product together with incompatible substances.
.
.

Page 8 of 9Issuing date: January 6, 201515. Regulatory information
Japanese Laws and Regulations:
Industry Safety and Health Law:
It is classified as a hazardous chemical. As for prevention for hazard, please see Article 20 to Article 36 of this law. As for theclassification criteria of hazardous and dangerous chemicals, please see Article 55 to Article 58 of this law.
Poisonous and Deleterious Substances Control Law: As for the whole product, no relevant classification according to thisLaw.
Fire-Fighting Law: There are no special requirements under this law as this product is not combustible.
The Law of Ocean Pollution Prevention: This product is classified as hazardous waste. Dispose it according to relevant local
or national laws and regulations.
PRTR: NoneInternational Laws and Regulations:
TSCA (Toxic Substance Control Act): 3,3′-Diaminobenzidine hydrochloride (CAS: 7411-49-6) is listed in the U.S. Toxic
Substances Control Act Chemical Substance Inventory List.
Clean Water Act:
None of the chemicals in this product are listed as Hazardous Substances under the CWA.
None of the chemicals in this product are listed as Priority Pollutants under the CWA.
None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
67/548/EEC AnnexⅠ:
Ingredient(s)Risk-phrase

Safety-phrase

Hydrogen peroxide（CAS: 7722-84-1）

R5-8-20/22-35

S(1/2-)17-26-28-36/37/39-45

Carcinogenicity categories: Not listed by EPA, IARC and NTP.

16. Other information
DISCLAIMER: Employers should use this information only as a supplement to other information gathered by them,and should make independent judgement of suitability of this information to ensure proper use and protect the healthand safety of employees. This information is furnished without warranty, and any use of the product not inconformance with this Safety Data Sheet, or in combination with any other product or process, is the responsibility ofthe user.

References:
GHS Technical Documents (4th and 5th Versions)
JIS Z 7253-2012
JIS Z 7250-2010
Industry Safety and Health Law
Poisonous and Deleterious Substances Control Law
Fire-Fighting Law
PRTRPage 9 of 9
Issuing date: January 6, 2015

Full description of some acronyms:
GHS-Globally Harmonized System of Classification and Labelling of Chemicals
CAS-Chemical Abstracts Service
EINECS-European Inventory of Existing Commercial Chemical Substances
ACGIH- American Conference of Governmental Industrial HygienistsOSHA-Occupational Safety and Health Administration
IARC- International Agency for Research on Cancer
NTP- The National Toxicology Program
IMO-International Maritime Organization
IMDG-International Maritime Dangerous Goods
IATA-International Air Transport Association
ICAO-International Civil Aviation Organization
TSCA-Toxic Substance Control Act

The latest version issuing date: January 6, 2015
SDS Version: 2*********************************The End **************************************