Page 1 of 9
Issuing Date: December 27, 2013

Safety Data Sheet
The content and format of this SDS is accordant with GHS standard.

1. Identification of the substance/preparation and of the company/undertaking
Product name:-Histofine® New Fuchsin substrate kit
Product code: 415161F
Recommended use of the chemical and restrictions on use: Research product. Immunohistochemical staining kit. For
professional use.
Restrictions on use: Do NOT use it in an application which may contaminate food or do harm to human health.
Manufacturer/Supplier: NICHIREI BIOSCIENCES INC.
Address: 6-19-20, Tsukiji, Chuo-ku, Tokyo, 104-8402, Japan
Tel.: +81-3-3248-2207
Fax: +81-3-3248-2243
Further information obtainable from: NICHIREI BIOSCIENCES INC.
Information in case of emergency:
Tel.: +81-3-3248-2207

2. Hazards identification
GHS classification:
Physical hazards

Health hazards

Environmental hazards

Explosives-not classified
Flammable gases-not classified
Flammable aerosols-not classified
Gases under pressure- not classified
Flammable liquids- not classified
Flammable solids-not classified
Self-reactive substances and mixtures-not classified
Pyrophoric liquids-not classified
Pyrophoric solids-not classified
Self-heating substances and mixtures-notclassified
Substances and mixtures, which incontact with water, emit flammablegases-not classified
Oxidizing liquids-not classified
Oxidizing solids-not classified
Organic peroxides-not classified
Corrosive to metals-not classified

Acute toxicity (oral)- 5
Acute toxicity (dermal)-not classified
Acute toxicity (inhalation)-not classified
Skin corrosion/irritation- 2
Serious eye damage/eye irritation-2A
Respiratory sensitizer-1
Skin sensitizer-not classified
Germ cell mutagenicity- not classified
Carcinogenicity-not classified
Toxic to reproduction- 2
Effects on or via lactation-not classified
Specific target organ toxicity following
single exposure-1 (respiratory organs)
Specific target organ toxicity following
repeated exposure-1 (tooth, respiratoryorgans)-2 (blood)
Aspiration hazard- not classified

Acute hazards to the aquatic
environment-2
Chronic hazards to the aquatic
environment-not classified
Hazard to the ozone layer-notclassified

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Issuing Date: December 27, 2013

Signal word: Danger
Symbol:Hazard Statement:
H303: May be harmful if swallowed.
H315: Causes skin irritation.
H319: Causes serious eye irritation.
H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H361: Suspected of damaging fertility or the unborn child.
H370: Causes damage to organs (respiratory organs).
H372: Causes damage to organs (tooth, respiratory organs) through prolonged or repeated exposure.
H373: May cause damage to organs (blood) through prolonged or repeated exposure.
Precautionary Statements:
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash hands thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P273: Avoid release to the environment.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P281: Use personal protective equipment as required.
P285: In case of inadequate ventilation wear respiratory protection.
Response Precautionary Statements:
P302+P352: IF ON SKIN: Wash with soap and water
P321: Specific treatment (see specific information on this label).
P362: Take off contaminated clothing and wash before reuse.
P304+P341: IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable forbreathing.
P342+P311: If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician
P305+P351+P338: IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy
to do-continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
P308+P313: IF exposed or concerned: Get medical advice/attention.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
Storage precautionary statements :P405: Store locked up.
Disposal precautionary statements :P501: Dispose of contents/container to relevant local and national regulations.

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Issuing Date: December 27, 2013

3. Composition/information on ingredients
Product description: substance (); preparation/mixture (√)(1) Reagent A: New Fuchsin solution concentrate
Ingredient (s)

CAS#

EC#

Official Notice
Reference
Number (Japan)

Water7732-18-5

231-791-2

6-204592.503%

Hydrogen chloride

7647-01-0

231-595-7

1-215

7.00%

Fuchsin, new3248-91-7

221-831-7

5-19700.50%% by weight(2) Reagent B: Activating reagent concentrate
EC#

Official Notice
Reference
Number (Japan)% by weight

Ingredient (s)

CAS#

Water

7732-18-5

231-791-2

6-204599.00%

Sodium nitrite

7632-00-0

231-555-9

1-483

1.00%

(3) Reagent C: Substrate buffer concentrate
Non-hazardous(4) Reagent D: Substrate solution concentrate
Non-hazardous

4 First aid measures
Persons using this product should consult a physician or other medical professional if an accident
involving this product results in injury. Specific first-aid measures are as follows:
Eyes Contact: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do andcontinue rinsing. If eye irritation persists: Get medical advice/attention.
Skin Contact: Immediately remove contaminated clothing and wash before reuse. Wash with water and soap. If skin irritation
occurs: Get medical advice/attention.
Inhalation: If discomfort occurs after inhaling vapour from processing, remove to a place where air is fresh and keep at rest ina position comfortable for breathing. If discomfort persists, get medical attention.
Ingestion: Rinse mouth. Do not induce vomiting without doctor’s instruction. If discomfort occurs, get medical attention.
Acute effect and delayed effect: Acute effect: It causes serious irritation to eyes and skin irritation. If inhaling concentrated
vapour of this product, symptoms may include and the upper airway edema, respiratory tract inflammations, necrosis, and alung blister. Delayed effect: It causes damage to organs (tooth, respiratory organs) through prolonged or repeated exposure. It
may affect blood.
Personal protective equipment: Wear protective gloves/clothing/safety glasses/respirators when necessary.
Indication of immediate medical attention and treatment needed, if necessary: Treat according to symptoms.

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Issuing Date: December 27, 2013

5. Fire-fighting measures
Extinguishing Media: This product is not combustible, as for fire caused by other ignition sources, use appropriate
extinguishing media according to fire types. Seek advice from local fire-fighting authority.
Specific Hazards Arising from the Chemical: It may release irritating and hazardous fume/gas from thermal decomposition.
Fire Fighting Method:
For initial fire, use dry powder, carbon dioxide, etc.
For large fire, it is effective to use fire foam, etc. to shut off air supply.
Firefighters must wear self-contained breathing apparatus and full protective equipment (e.g. fire-retardant clothing).
Deny unnecessary entry to the place around the fire.
Remove containers from fire area if it can be done without risk.
Extinguish fire from upwind, and the fire extinguishing method should be appropriate to the situation in the surroundings.
Fire Fighting Equipment: Firefighters must wear self-contained breathing apparatus and full protective equipment.

6. Accidental release measures
Personal precautions: Use proper personal protective equipment as indicated in Section 8.
Measures for Cleaning/Collection:
Small Spill:
Mop up or absorb with an inert dry material (e.g. sand, dry lime) and place in an appropriately labeled waste disposal
container.
Large Spill:
Seek professional advice from companies dealing with waste disposal if necessary.
Isolate contaminated area and set up a warning board. The persons handling emergency are recommended wearing protective
clothing. Do NOT touch spilled material. Build up dike or dig pit for collection. Collect the spilled material into a labeledcontainer and keep it tightly sealed.
Environmental Precautions: Keep cleaning run-offs out of municipal sewers and open bodies of water. Comply with local
and national laws and regulations.

7. Handling and storage
Handling:
Workers must wear proper protective equipment and must operate strictly according to relative rules.
Do not breathe vapour or fume that may be evolved during processing.
Information about fire- and explosion protection:
No special requirement for this product.
Storage:
Requirements to be met by storerooms: Store it in a well-ventilated, cool and dry store room. Avoid long-time direct
sunlight. Store locked up.
Additional Information: The storeroom should be equipped with proper facilities for accidental leakage and fire.

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Issuing Date: December 27, 2013

8. Exposure controls/personal protection
Occupational Exposure Limits:
IngredientsOSHA
PEL-TWAACGIH TLV-TWA

Japan Industry & Health
Association-PELs

Hydrogen chloride (CAS : 7647-01-0)

5 ppm

2 ppm

5 ppm

Engineering Controls:
Use process enclosures, local exhaust ventilation, or other engineering controls to keep airborne levels below recommended
exposure limits.
Personal Protective Equipment (For workers) :Protection of Hands:
Wear protective gloves. (e.g. impermeable rubber gloves)Protection of Eyes:
No special requirements under normal conditions. Wear safety glasses when liquid may contact eyes.

Respiratory Protection: Use an approved respirator if exposure limits are exceeded or if irritation or other symptoms occur.

Protection of Body:
Recommend wearing uniforms made of antistatic synthetic fiber

General protective and hygienic measures:
Keep away from foodstuffs, beverages and feed.
Wash hands before breaks and at the end of work.
Avoid contact with eyes and skin.
Wash contaminated clothing before reuse.

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Issuing Date: December 27, 2013

9. Physical and chemical properties
General Information
Form

LiquidColour

New Fuchsin solution concentrate (Reagent A): Ocher
The other reagents except above: Colorless and transparent

Odour

Odourless

pH Value

No data available

Boiling Range

No data available

Melting point/Melting range

No data available

Flash point

No data available

Flammable/Explosive Limits-Lower Vol %Not applicable

Flammable/Explosive Limits-Upper Vol %Not applicable

Density

No data available

Relative Vapor density

No data available

Vapor pressure

No data available

Solubility in/Miscibility with Water

Soluble in water.

n-octanol/Water Partition Coefficient

No data available

Self-igniting

Not applicable

Decomposition Temperature

Not data available

Odor Threshold ValueNo data available

Evaporation Rate

No data available

Flammability (solid, gas, etc.)

This product is not classified as flammable liquid.

10. Stability and reactivity
Chemical Stability: Stable under normal temperatures and pressures.
Hazardous Reactions: Not found.
Materials to avoid: Cyanide, metal powder, alkalis, etc.
Hazardous Decomposition Products: It may release irritating and hazardous fume/gas from thermal decomposition.
Conditions to Avoid: Long-time exposure to sunlight.

11. Toxicological information
Product Toxicity Data: The toxicity data of this product has not been determined by testing or research, but to our best
knowledge and reference, the toxicity of this product is extremely low. The toxicity data shown below is for reference only.
IngredientsCAS

LD 50 /LC 50 (Median lethal dose)

Hydrogen chloride

7647-01-0

Acute toxicity (Oral) LD50: 238-277mg/kg，700 mg/kg(rat)
Acute toxicity (Dermal) LD50＞5010mg/kg (rabbit)
Acute toxicity (Inhalation: vapour) LC50: 4.2, 4.7, 283

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mg/L/60min
Acute toxicity (Inhalation: aerosol) LC50: 1.68 mg/L/1h
Sodium nitrite

7632-00-0

Acute toxicity (Oral)LD50:85 mg/kg,180mg/kg (rat)Acutetoxicity(Inhalation:
dust,
mist)
LC50:
3
0.0055mg/L/4H (5.5mg/m /4H)

Serious eye damage/eye irritation: This product is classified into Category 2- skin irritation.
Skin corrosion/irritation: This product is classified into Category 2A-seriouseye irritation.
Respiratory /Skin sensitizer: This product is classified as respiratory sensitizer.
Germ cell mutagenicity: No classification for this product.
Carcinogenicity: No classification for this product.
Reproductive Toxicity: This product is classified into Category 2- suspected of damaging fertility or the unborn child.
STOT SE/RE:
single exposure-1 (respiratory organs)
repeated exposure-1 (tooth, respiratory organs); -2 (blood)
Aspiration hazard: No classification for this product.

12. Ecological information
Ecotoxicity: No relevant data for the whole product, the data shown below is of the ingredient.
Hydrogen chloride (CAS : 7647-01-0):
48-hour EC50=0.492mg/L of Crustacea (Daphnia magna) (SIDS, 2005).
Sodium nitrite (CAS: 7632-00-0):
96 hours LC50: 0.54mg/L of the fish (Rainbow trout) (SIDS, 2006）
Persistence and Degradability: No data available.
Bioaccumulative Potential: No data available
Mobility in Soil: Due to its liquid status, it can move in soil.

13. Disposal considerations
Minimize the hazard of waste by the methods of neutralization and stabilization.
Any disposal practice must be in compliance with country, local, state, and federal laws and regulations.
After contents are completely removed, dispose of its container at hazardous or special waste collection point.
Paste a label on the container indicating the possible hazards of the waste.

14. Transport Information
DOT/Air-Transportation- IATA/ICAO/Sea-Transportation-IMO/IMDG:
Proper Shipping Name: Not regulated.
Hazard Class: Not classified.

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UN Code: Not regulated.
Packing Group: Not classified
Packing Group Symbol: Not classified.
Marine Pollutant (Yes/No): No
EMS NO.: Not regulated.
Note:
Check whether the package is completed or sealed before transporting; make sure there are no damage and no falling down of
packages during transporting; the transport vehicle should be equipped with fire-fighting equipment and emergency facilities
for handling with release; do NOT transport this product together with incompatible substances.

15. Regulatory information
Japanese Laws and Regulations:
Industry Safety and Health Law:
It is classified as a hazardous chemical but it may have potential hazard to health. As for prevention for hazard, please see
Article 20 to Article 36 of this law. As for the classification criteria of hazardous and dangerous chemicals, please see Article
55 to Article 58 of this law.
Notified information of hazardous and dangerous substances under this law:
Ingredients

Range (%by weight)

Law or government ordinanceHydrogen chloride (CAS : 7647-01-0)≥0.1

Government ordinance No.18.2-No.98 in table 9

Poisonous and Deleterious Substances Control Law: As for the whole product, no relevant classification according to thisLaw.
PRTR: None of the ingredients of this product are listed as specified chemicals under this law.
Fire-Fighting Law: It is not combustible. No relevant classification.
Safety of Ships Law: It is classified into class 9 (Environmentally hazardous substances, liquid (containing hydrogenchloride). Comply with relevant provisions of this law.
Aviation Law: It is classified into class 9 (Environmentally hazardous substances, liquid (containing hydrogenchloride). Comply with relevant provisions of this law.
The Law of Ocean Pollution Prevention: Hazardous waste. Avoid release to environment.
International Laws and Regulations:
TSCA (Toxic Substance Control Act): All ingredients of this products are listed in the U.S. Toxic Substances Control Act
Chemical Substance Inventory List.
67/548/EECAnnexⅠ:
Ingredients

Risk phrases

Safety phrases

Hydrogen chloride (CAS : 7647-01-0)

R: 23-35

S: (1/2-)9-26-36/37/39-45

Sodium nitrite (CAS: 7632-00-0)

R: 8-25-50

S: (1/2-)45-611

Clean Water Act:
Hydrogen chloride (CAS : 7647-01-0) and Sodium nitrite (CAS: 7632-00-0) are listed as Hazardous Substances under the
CWA.
None of the chemicals in this product are listed as Priority Pollutants under the CWA.

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Issuing Date: December 27, 2013

Carcinogenicity categories: Not listed by EPA, IARC and NTP.

16. Other information
DISCLAIMER: Employers should use this information only as a supplement to other information gathered by them,and should make independent judgement of suitability of this information to ensure proper use and protect the healthand safety of employees. This information is furnished without warranty, and any use of the product not in
conformance with this Safety Data Sheet, or in combination with any other product or process, is the responsibility of
the user.
References:
GHS Technical Documents (Version 4 and Version 5)
JIS Z 7253: 2012
JIS Z 7250-2010
Industry Safety and Health Law
PRTR
Poisonous and Deleterious Substances Control Law

Full description of some acronyms:
GHS-Globally Harmonized System of Classification and Labelling of Chemicals
CAS-Chemical Abstracts Service
EINECS-European Inventory of Existing Commercial Chemical Substances
ACGIH- American Conference of Governmental Industrial Hygienists
OSHA-Occupational Safety and Health Administration
IARC- International Agency for Research on Cancer
NTP- The National Toxicology Program
IMO-International Maritime Organization
IMDG-International Maritime Dangerous Goods
IATA-International Air Transport Association
ICAO-International Civil Aviation Organization
TSCA-Toxic Substance Control Act
The latest version issuing date: December 27, 2013
SDS Version: 1*********************************The End **************************************