The 13th Conference on

Fungal Genetics
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qx¼¢¯HgG

11 月２０日（月）

９:30 -

受付開始（於：講演会場）

10:30 - 10:40

開会の辞

10:40 - 13:00

口頭発表（O-1〜12）

13:00 - 14:20

休憩

14:20 - 15:20

特別講演

15:20 - 16:40

ポスター発表（奇数番号）

18:00 -

懇親会

11 月 21 日（火）9:00-

受付開始（於：ポスター会場）

9:30 - 10:50

ポスター発表（偶数番号）

11:00 - 12:40

口頭発表（O-13〜20）

12:40 - 14:00

昼休み

14:00 - 16:00

シンポジウム16:10 - 16:40

総会・表彰式

16:40 - 16:50

閉会の辞- 2 —

発表演題および講演時間
特別講演

11 月 20 日（水）14:20 - 15:20

糸状菌における菌体外消化システム：分解酵素の分子機構から見えてくるカビ・キノコのバイオマス資化戦略
東京大学

大学院農学生命科学研究科

五十嵐 圭日子
座長：阿部 敬悦

シンポジウム（東北大学）

11 月 21 日（木） 14:00 - 16:00「糸状菌の多様性〜種、ゲノム、機能」[座長：S-1, 2：田中 千尋（京都大学）；S-3, 4：本山 高幸（理化学研究所）]

14:00-14:30
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14:30-15:00
S-2
Dictyocatenulata alba  ]¦ N     Rhw4#&90?./=1?rz²j                          kM
15:00-15:30
S-3¢·4=(P       lbhh¥w¢ht/=1?
                          es
15:30-16:00
S-4
7.2*38|F¢DF©¬Uv¯Hg
Vi P      b³bh mh[´E/=1?    ~A- 3 —@¨¹O-1O-12º 11 } 20 {¹º 10:40 - 13:00

ɣ֗‫ ׾‬O-1, 2: ͅȱ Ģú‫װ‬тΈǱ‫ ;ױ‬O-3, 4, 5: ʉʹ ĺô‫װ‬ƜǔǱ‫ ;ױ‬O-6, 7, 8: Ԧ ĥ΂‫װ‬Ǳ֞ɡǱ‫ױ‬O-9, 10: οԦ ‫ׅ‬Ò‫װ‬Ǳ֞ɡǱ‫ ;ױ‬O-11, 12: ҉Ϩ ĭ‫װ‬տѼЗ‫ ;ױ‬O-13, 14, 15: Ýȹ γÒ‫̭װ‬õǱ‫ױ‬O-16, 17: ȫоƆ Ρ‫֗װ‬ȻЧʮǱ‫ ;ױ‬O-18, 19, 20: ΅΍ ɬР‫װ‬ίԴЍǱ‫ױ‬
10:40 O-1 јϒӑgѧҥԺ×Ʃƙʏƍʊ̈eFM|µÊÆ¾ѧҥ͓ͦ͜gӽ̰Õ͉ƵǱ 1‫ײ‬ðÕŧǸȆ 1‫̡ײ‬Ϩź̎ 1‫ײ‬Üȵ˰ǐ 1‫ײ‬ň̮ҜȆ 2‫ײ‬ͺϨĭÒ 1‫װ‬1 СʦǱ֤ÏՎȋЗгЧ‫ײ‬2 ̲͚З‫ױ‬

10:52 O-2 ‫ש‬ӑ A. oryzae gӑј˗ĴɼgѧҥĥʀeFM| AoSO g͜Ҧӽ̰Ʌϯѯɗ‫ײ‬ĐĉǍ‫ײ‬ÝȹγÒ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬

11:04 O-3 ˶Ӹí͟ĀԠŘɿƿȆbS`g Aospt3 g͜Ҧӽ̰ɧλְ÷ 1,2‫΀ײ‬ŉԥá 1,2‫ײ‬ȼÖƩӯ 1,2 ‫װ‬1 ɜȿǱ֤ÏļлЗ‫ײ‬2 տѼЗ‫ױ‬

11:16 O-4 Chaetomium globosum eFM|í͟ĀԠb²«¤¢ǫůg֛ ՠʏÜͿȂ÷ 1‫ײ‬ЖŉŰÒչ 2‫ײ‬ïƍϨɦȊ‫ײ‬ֈƒƀƗ‫ײ‬ΥՏԸí‫־װ‬ȻЍǱ֤Ӟ, 1 ϝ õǱ֤Վ, 2 ϝ ˻̤ǱӞ‫ױ‬

11:28 O-5 Ç‫׌‬οϸϺӑeFM|Őӄցͯ RAM «¢¦ËÐ͓ʣƿȆ CoPag1 h͋ϏϐϴЃ¨ÈƐȚúSZþАƺɳʣe֛ØU|ȫϚџĀ‫ײ‬Ǐƒ ͤ‫ײ‬àģɦá ‫װ‬õռɡǱ֤Ïϥϣ‫ױ‬

11:40 O-6 Trichoderma reesei eFM|ÈÆÐϥϦe֛ØU|¦ÆÌ»ÐÐgӽ̰ԦƒǱ͚‫˻ײ‬ÖФӧ‫ײ‬ƓɅ֩њ‫ײ‬ʉϨΌú‫ײ‬ȫоƆΡ ‫֗װ‬ȻʮЧǱÏϥϏ‫ױ‬

11:52 O-7 Trichoderma reesei eȉǌU| 10 Ю‫׌‬gβ-glucosidase isozyme gցѣŴȋЃʏԺջ ƀΌ‫ײ‬ֈ‫׮‬ŦÒ‫ʹײ‬ֈ͢Γ‫ײ‬ǲֈҾɴ‫װ‬ĤɆǱ֤Ïϥƨ͜ҦÏ³

12:04 O-8 ‫ש‬ӑeFM|ҷɌŒ˴ǒ Cle/loxP ձʴ¼ÐÐÇ®ÐɈȋ‫ױ‬ÇÌ¤¾gЛи

ɯ˵̫‫ײ‬Ċ̐ɴ‫ײ‬͹ƆЊ͚‫ʹײ‬Ԧ΂ 1)‫ײ‬ϨÜϡɌ‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå‫̭װ‬ŵǱ֤Վ‫ײ‬տѼЗ 1‫ױ‬

12:16 O-9 јϒӑ Trichoderma reesei eFM|ӑĒǭ҇ѸϒϏԺƙʣցѣgˍѤϨƆċʓ 1‫˶ײ‬ϨҍԲȆ 2‫ײ‬ʉϨΌú 1‫ײ‬ǙɅТϥ 3‫̦ײ‬ȹΜƗ 3‫ײ‬Ǳ֨͢Ȇ 4‫ײ‬ȫоƆΡ 1‫װ‬1 ֗ȻʮЧǱÏϥϏ‫ײ‬2 ЧȋʮӨ˄Һ͓͜‫ײ‬3 õռǱȋÏϥȉǇЗгʩ‫ײ‬4 ѷƙϭķЗгˢ˖‫ױ‬

12:28 O-10 3D À¥ÇÌŗϧSZʲϥϏԺÆÐgǫϴĒԄԀÜȹƩͮ‫ײ‬ȹϨְ÷‫ײ‬ӴϨΌɍ‫ײ‬МԩΊɬ ‫װ‬ȞȹЍǱÏϥɈ‫ױ‬- 4 —

12:40 O-11 Ǯ͔dјϒӑ‫׌‬g SSH Æ´ÆÇÐӽ̰ey|®

¼ѕŴ֛ՠղĈȆʗǝgƎֱȎƴ͒‫ײ‬ȫƒ̉ǵ, ɏɅǬȆ, ֛Ʌ̏Ό, ѡֈǍ‫װ‬NITE‫ױ‬

12:52 O-12 ּҿĽřηØE`јϒӑGz̚ϧϏԺϥϦU|ȫɄ˦Ĥ‫ײ׶‬ÔΒйɗ‫ײ׶‬ÔƆҜ‫׷‬8ɏ̨́њ‫׶װ ׷‬ĤɆǱ֤Վ‫ ׷ײ‬Üֈɏ‫ױ‬@¨¹O-13

O-20º 11 } 21 {¹~º 11:00 – 12:40

11:00 O-13 A. flavus A.oryzae ӑ̼Ҏg¬¾Ë§dљѱӽ̰

ǱϨ Ͳ‫҉ײ‬Ϩ ĭ‫ײ‬ȼÖƩӯ‫ײ‬ɼӟǸҍ‫װ‬ϔиӧ˦·÷ տ‫׌‬ѼƙЗгʩ օ՟ʮӨǗЇЗгպ֘‫ױ‬

11:12 O-14 ©¦Ê¡«ÌgϿӷgXg  ҘʏŴ͓͜gӽ̂ƥŲ˨‫פײ‬СȤ̇, ̵ȱĢú, ‫מ‬ԦЊ͚‫װ‬тΈǱ֤ÏϥƨϣǢ‫ױ‬

11:24 O-15 ¼h‫ײ‬ՔѥǭĽHӷE|Ħ͛ˬ 1‫ײ‬ÖϨ֩ƕ 1‫ײ‬ĐӟЎá 1,2,3‫ײ‬ӟ͉˱Ƴ 2,3‫ײ‬ɗ֚ΜƗ 4‫ײ‬ӴǙҙÔ 1 ‫װ‬1 ַǅrCZM,
2­³«‫ײ‬3 ̭õș˦ǱÏϣǢ˪ҡ‫ײ‬4 ֊ͿɈǱÏֹͳ/Ľ/«ÈÐʊϧ‫ױ‬

11:36 O-16 ձʴЃЁҿҬ̥ӑ Ceriporiopsis subvermispora g®©ÇÌeȣU|ѧҥʊуΥշɀ÷‫͉ײ‬ԸÒչ‫̡ײ‬ʦɵȆ‫֗ײ‬ԦɅ֩Ǳ‫ײ‬Ӵ̨ӯʉ‫̤ײ‬ϨØÒ‫ײ‬ΥՏ֩Ɨ‫װ‬õǱÏϥȉЗ‫ױ‬

11:48 O-17 ‫ש‬ӑÉ¡Ì͔Ì¯Ժ CreD gҪÇÌփŴey|ÈÐʰŘgŘɿϨÜϡɌ‫ײ‬ɗ̤Ƭå‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå‫̭װ‬ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ױ‬

12:00 O-18 Cv\ϺӑeFM|°¦ÌÇÌ¿¡È¦ÆÌ³ÆÐgɷŢ
Kieu Pham Thi Minh, ðÕҏƀ, Ba Van Vu, Quoc Bao Nguyen, ͺϨĭÒ, Üȵ˰ǐ‫װ‬СʦǱÏՎ‫ױ‬12:12 O-19 ϺƆЎӑ Aspergillus fumigatus gÐÈŞҘʏeFM| AtrR gǮ͔d͜ҦӕƆǱР 1‫ײ‬ǱǞͤ 2‫ײ‬ΟʹŃʃ 1‫ײ‬Ʌ̤բ 1‫ײ‬ïƦŲå 2‫װ‬1 źӗǱÏЎӑÌÐ‫ײ‬2 ̭ŵǱ֤ÏՎ‫ױ‬12:24 O-20 Ç‫׌‬οϸϺӑbțÞÂÇ֚eɳʣQ}|ÇÌϒ³ÐՍՙe֛U|ӽ̰ŀ̳Ί͚‫ײ‬ŜϨ В‫מײ‬ֈҏȊ ‫װ‬õǱÏ֤ÏՎ‫ױ‬- 5 —

ÌÐ³

eƝMZ

7.1?§ 11 } 20 {¹ º15:20 – 16:40¹dyTº11 } 21 {¹~º 9:30 – 10:50¹KyTºP-1Őӄցͯ Sln1p ΦɢʜƐʏ̼eFM|͋Ϗ-Ўӑӥƙ°¡Ì¨Ðg͜ҦЋӰb¡ÉÌʊуÜȹЎϩҍ 1,2‫ײ‬ƓɅĭǳչ 3, ƚӷƳ 1, ֡պ˭ʕ 1,2‫װ‬1 ̭ŵǱÏ̢̫З, 2 ̭ŵǱÏ֤ՎÏϥϏϦ͏ţʣ, 3 Univ. of Gothenburg, Dept. of Chemistry and Molecular Biology‫ױ‬

P-2н̧gǛвŏϟHѧӑҎֱ͓՟eØE|ɶ‫׃‬
1P-3

ÜϨӯ΂, 1 ƚϨ Ԕ, 2 ‫מ‬ϱəƗ‫װ‬1 ՎɈǱÏՎ‫ײ‬2 ȞȹЍՎ̱ʹϦѼƙʮӨÌÐ͉̱Згʩ‫ױ‬11 ЮgЁҿFykӳҿ̡̧Ҭ̥ Polyporales g¬¾FykÉ¦Ð¾ӽ̰
Ǚ ź̂‫װ‬ϟЗÏCSRS‫ײױ‬ïŹɃǍ˻Ȇ‫סײ‬ȿ͢Γ‫̭װ‬Ǳ֤ÏՎ‫ײױ‬David Hibett‫װ‬ÆÐǱ‫ײױ‬Bernard
Henrrissat(CNRS)‫ײ‬Jill Gaskel ‫ײ‬Dan Cullen (IMBT, FPL)

P-4

‫ש‬ӑeFM|ҷɌŒ˴ǒ Cle/loxP ձʴ¼ÐÐÇÇÌ¤¾gЛи

1)ɯ˵̫‫ײ‬Ċ̐ɴ‫ײ‬͹ƆЊ͚‫ʹײ‬Ԧ΂ ‫ײ‬ϨÜϡɌ‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå‫̭װ‬ŵǱ֤Վ‫ײ‬տѼЗ 1‫ױ‬
P-5

͐ʂ։Ԋ˳ϧCZ˱ŴԫeβǌU|јϒӑgÀ©ÇÌƩϨ̛Ȇ 1‫ײ‬ïŹɃǍ˻Ȇ 2‫ײ‬ӟƆӯȆ 3‫ײ‬ӟðҏà 3‫ײ‬ȻϨ ĭ 1‫װ‬1 ̭õ˱ŴԫЗгʩÏģĥ‫ײ‬2 ̭Ǳ֤ÏՎϥЧ‫ײ‬3 õǱ֤ÏՎЧ‫ױ‬P-6јϒӑϩ̫gľϷƾմ͜Ҧʏѣ̧ϧCZ˶ӸŸϽϧ¨¬ђȆg֙Ͽ ĐӟǱԲ ̨̮‫ח‬Ӓ ‫͛מ‬ʞǳչ ‫͛מ‬ʅ̨ǓŃӆ ЖðʒȆ ɅÕƩҏ ō͙Բʣ ‫מ‬ӷԔÒ ֡Ȱְ˱ У̤ȋ ֡պ˭ʕ  ‫̭ װ‬ŵǱ֤Վ ̭ŵǱ֤Ÿ ̭ŵǱÏŧ‫׭‬З ȹɳǱϟ ̭ŵǱÏǮĺЗ ̭ŵǱÏ̢̫З‫ױ‬P-7°ÆӑјeּҿĽřηØE`ʲϾΎʏϏԺϥϦU| ÔΒйɗ‫ײ׶‬ʦϨÒɭ ‫ײ‬ӟðƀ ‫ײ‬ȫɄ˦Ĥ‫ײ׶‬ÔƆҜ‫ײ׷‬ɏ̨́њ‫׶װ ׷‬ĤɆǱ֤Վ‫ ׷ײ‬Üֈɏ‫ױ‬P-8Ê®¤Ç¾·ey|ӑ̾ӑ¹Ì¿gղĈȆѪ˔̼gĕŐ ȹ̤Ўɪ 1‫΅ײ‬΍ɬđ 2‫ײ‬ŵŐ֯ϥ 1‫ײ‬ҐϵŧǸȆ 1‫ײ‬ϨÜźȨ 1‫װ‬1 õǱ֤ÏՎ‫ײ‬2 ίԴЍǱÏϣǢЧȋ‫ױ‬P-9‫ש‬ӑ Aspergillus oryzae Ð¦³Ð͞˗̼eFM|ϴЮÌ¯ԺϥϦʏgƝÕȢ̤ Ƞ, Ƙű Οҍ, ȫȿ Ζɗ, ȌϨɅ ӯ̉, ‫ ̡ן‬ʈ, Ýȹ γÒ 1, ŵ̤ ŲjO 1‫¬װ‬º

¾ Á¯Ì‫ױ̼װ‬, 1 ̭Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬P-10 ‫ש‬ӑϧCZƛ̈ѕŴϿցey|¥ÌµÌGzg L-çփϥϦӅð ̐ 1‫ײ‬ƚ̻ĢФ 2‫ײ‬ΐðӒ:Ȕ 1‫ײ‬ȹϨöР 1‫ײ‬ӎֈźЦ 2‫ײ‬ǜ ΓȆ 3‫ײ‬Щ Όí 3‫ײ‬Քӟ̇ɴ 2‫װ‬1 СʦǱÏҷτЧȋ‫ײ‬2 СʦǱÏɈ‫ײ‬3 ̙́ŌÏѼЗ‫ױ‬
P-11 јϒӑ PKC g˶Ӹ֠ȘŞeȣU|ցͯÇÐ©Ìљg͓ь ɝƗոǵ 1‫ײ‬ÜȹЎϩҍ 2‫ײ‬ƚӷƳ 2‫ײ‬ӟȻ̏Ś 3‫΀ײ‬ƙΟ 3‫ײ‬Ǚŉӯá 4, ͆ȹФ̂ 5, ֡պ˭ʕ 1,2‫װ‬1 ̭ŵǱ֤ՎÏϥϏϦ͏ţʣ, 2 ̭ŵǱÏ̢̫З‫ײ‬3 ½- 6 —

ŴȋɈ͏, 4 ̭Ǳ֤Վ, 6 ÜǵǱϟɈ‫ױ‬

P-12 ‫ש‬ӑѼƙ¬¾¥Ð·Ðg֙ϿbŃ֙ ҉Ϩĭ 1‫ײ‬ÕϨΊǵ 3‫ײ‬ȼÖƩӯ 1,2‫װ‬1 տѼЗ‫ײ‬2 ɜȿǱÏļлЗ‫ײ‬3 ̼ɩćП¨Ç‫ױ‬P13

Aspergillus oryzae g͜Ҧʏ¸µ¡§ӥƙ­§Ê³Ð±Ì‫ װ‬HypB‫ױ‬ey|ѧҥӪȸɈȋ¤¾g͓ь
Ǳ͛Ĥǳչ‫ײ‬ǚŜǍđ‫ײ‬Ü֚Қ‫ײ‬ŧλ̂˻‫ײח‬Üȿ̅ѥ‫΂̂װ‬ǱÏՎÏՎŴ‫ױ‬
P14‫ש‬ӑ A. oryzae ϧCZ͋Ϗϩ̫À¡ÌgϥϦFykєӱąӟǱĥ‫ײ‬ÝȹγÒ‫͵ײ‬ϨȐ͟ 1‫ײ‬ϨáĦĸ 1‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ײ‬1 ̭Ǳ֤ÏՎϥЧÏʊϥŴ‫ױ‬

P-15 јϒӑ Aspergillus nidulans α-1,3-ÈÌ͞˗̼gǖ‫ה‬ϐʏbϏԺϥϦʏgԉĝ Ò̦́Ϙ 1‫ײ‬ƚӷƳ 2‫ײ‬Яӗ͇ 1‫֡ײ‬պ˭ʕ 1,2‫װ‬1 ̭ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ײ‬2 ̭ŵǱÏ̢̫З‫ױ‬
P-16 ΁їʂϥϏ Mortierella alpina 1S-4 g lig4 ղĈȆИǦ̼gĕӱӐɅȠƕ 1‫͝ײ‬Ԧӆ΂ 1‫ײ‬Ȏӟ̉Ӹ 1,2‫ײ‬ӖƙҍĐ 3‫ײ‬Οʹ ́ 4‫ײ‬ȫɅ ‫ ׅ‬1 ‫װ‬1 õǱ֤ՎÏʊϧϥƨ‫ײ‬2õǱÏϥϟŴȋÃ©¢¦‫ײ‬3 Ì¦ÇÐÊÐ®ÈǱÏ®

¬·ÐÄÌÌÐ̼ɩćП‫ײ‬4 õռȋǈϣǢ‫ױ‬P-17 Aspergillus ȷӑgѧҥǤ&-1,3-ÈÌgÇѕŴbXg͜Ҧʏԉĝ ƚӷƳ 18Яӗ͇ 28ГֈʣƩ 38Đ:̡Ȑ̂ 48ɼӟ̏ϥ 48ɅÕƩҏ 58֡պ˭ʕ 1, 2
(1 ̭ŵǱ̢̫З, 2 ̭ŵǱ֤Վ, 3 ȹɳǱ֤ϟɈ 4ÑȺ֝Æ£ӱ՟ʩ, 5 ̭ŵǱ֤Ÿ)
P-18 ‫ש‬ӑaϴЮϿϝQWZ³¥ÇÌϥƙʣցѣgѧҥŉȳǌӽ̰ Ċ̓ɴ‫ײ‬ϨÜϡɌ‫ײ‬ӟȴϢɱ#⁾‫ײ‬žэʉ#⁾‫ײ‬ƊɅӆЦ#⁾‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå‫̭װ‬ŵǱ֤ÏՎÏϥϏϦ͏ţʣ‫ײ‬ŵǱ֤Ïϟ‫ױ‬
P-19 Involvement of lectin-type cargo receptors in heterologous protein secretion in Aspergillus oryzaeDung Huy Hoang, Jun-ichi Maruyama, Katsuhiko Kitamoto (Dept. of Biotechnol., The Univ. of Tokyo)
P-20 ‫ש‬ӑaЛԐQ}Z 6 ħgÈ CoA ƙʣցѣ¹ÀÊgϥҡeFM|ɷŢgӽ̰ ϚֈȊÒ 1‫ײ‬Kenneth Bruno2‫ײ‬ȫͺӆ̂ 1‫ײ‬Жð̏Ȇ 1‫ײ‬ÔΒʛ 1‫̨͆ײ‬һȆ 1‫ײ‬Scott Baker2‫ײ‬ϬϨְá
1‫װ‬1 ϦѼЗÏϥϏµÊ‫ײ‬2 яǅ¯³¢¬Ð¦ǅиЗгʩ‫ױ‬

P-21 ‫ש‬ӑ A. oryzae eFM|¦ÉʊуԙъÌ¯Ժ AoRim15 g͜Ҧӽ̰Ų̈ӆΜ‫ײ‬Ӑ֚֩ʉ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬
P-22 јϒӑ Aspergillus nidulans ey|Ǌǧ³½ÌփgĀԠ͓͜ÜεǸҍ‫ײ‬ҕ΃ЗÒ‫מײ‬ԦЊ͚ ‫װ‬тΈǱ֤ÏϥƨϣǢ‫ױ‬
P-23 ‫ש‬ӑgӑјӥƙҦťgӽ̰˷·gЛиbǖǎʣőHØE|ɶ‫׃‬g͌ԁǠɁƩĖȆ‫ײ‬ÝȹγÒ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬
P-24 Aspergillus oryzae eFM| 331-25 sense RNA, 331-25 antisense RNA g͜Ҧӽ̰Րðְ‫͉ײ‬ϨȠ÷‫ײ‬ŜϨΓ‫ײ‬ȹɳΌɗ‫ײ‬нŉժ֯ ‫̭װ‬õՎɈǱÏʊϥЧ‫ױ‬- 7 —

P-25 ‫ש‬ӑ A. oryzae eFM|ӑ̽ɳʣʰŘƿȆ EcdR ͞˗ey|̚ʏϥͪϿӷgԊsϨÜ ŭͳ‫ײ̬֎ ֊ײ‬Ýȹ γÒ‫ײ‬ŵ̤ ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬
P-26 ‫ש‬ӑ A. oryzae eFM|Ð¦³Ð֛ՠҲÌ¯Ժ AoAtg9 gȳǌFyk͜Ҧӽ̰ӟ̡ҙɗ‫ײ‬Ӑ֚֩ʉ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬
P-27    gѧҥǤ¦ÉҘʏe֛|͜Ҧ̢ДղĈȆgӽ̰ ʃ͵Ǹǵ ‫ײ‬ǾȱƕȆ  ‫ײ‬íСΊǗ ‫ײ‬нɅӝ ‫ײ‬ȼÖƩӯ  ‫ײ‬ɼӟ͢ŗ ‫ װ‬äǱ֤ÏϥԵϣ‫ ײ‬äǱ֤ÏՎ‫ ײ‬ɜǱ֤؊ļлЗ‫ ײ‬տѼЗ‫ױ‬


P-28 ‫ש‬ӑ½¬փ¦ÆÌ»ÐÐg͜Ҧӽ̰ ΑÜǱǰ‫֊ײ‬Ȇ̂ӯ‫ײ‬ŵɅ΂ʒ‫ײ‬Đֈĺ̇‫ײ‬ȱ֛ĭí‫ײ‬ǱщĤÒ (֊ͿɈǱÏ¬¾З)
P-29 Aspergillus nidulans eFM|µÊ¤Ì¨Ð C g sterigmatocystin ƙʣŘɿe֛|͜Ҧgӽ̰όȹϠå‫ײ‬Ǚŉӯá‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬
P-30 Aspergillus nidulans eFM|őϥȆϐϴЃĎőȆÌ¯Ժ CON ¹ÀÊgӽ̰֋̡Ҝ a‫ײ‬b‫ ײײ‬Özlem Sarikaya Bayrama‫ ײ‬Özgür Bayrama‫ ײ‬Gerhard H. Brausa‫װ‬a ¢¤ÌÌǱȋ‫ ײ‬b ‫׏‬ѼЗ‫ױ‬
P-31 Aspergillus nidulans gÇÐÌĀԠb¤Ç¼¦¡Ìϥƙʣ ȫ̮Ɂʛֆ‫ײ‬Ǫ̃̔ú, ‫מ‬ԦЊ͚ ‫װ‬тΈǱ֤ÏϥƨϣǢ‫ױ‬P-32 ‫ש‬ӑ¼È¦Ð¯Ð½ÐőӽeFM| HECT Ã±¡ÌÇÐ HulA g֛Ø̮ΒĸĖ‫ײ‬ϨÜϡɌ‫ײ‬ɗ̤Ƭå‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå ‫̭װ‬ŵǱ֤ÏՎÏϥϏϦ͏ţʣ‫ױ‬
P-33 јϒӑgѧҥԺ×Ʃƙʏƍʊ̈eFM|µÊÆ¾ѧҥ͓ͦ͜gӽ̰ 1Õ͉ƵǱ‫ײ‬1 ðÕŧǸȆ‫ײ‬1 ̡Ϩź̎‫ײ‬1 Üȵ˰ǐ‫ײ‬2 ň̮ҜȆ‫ײ‬1 ͺϨĭÒ‫װ‬1 СʦǱ֤ÏՎȋЗгЧ‫ײ‬2 ̲͚З‫ױ‬
P-34 ‫ש‬ӑ A. oryzae eFM| 2 ^g acyl-CoA binding protein (AoAcb1‫ײ‬AoAcb2)gӽ̰ɅƒҼɗ‫ײ‬Ӑ֚֩ʉ‫ײ‬ŵ̤ŲjO ‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬
P-35 ‫ש‬ӑ A. oryzae gӑј˗ĴɼgѧҥĥʀeFM| AoSO g͜Ҧӽ̰Ʌϯѯɗ‫ײ‬ĐĉǍ‫ײ‬ÝȹγÒ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬
P-36 Aspergillus oryzae g­§Ê³Ð±ÌҎg͜ҦFykȳǌʏgӽ̰ ȹɅѬ‫ײ‬ЖϨźѲ‫˽ײ‬ɅӀđӓ‫ʹײ‬ֈđ‫ײח‬Üȿ̅ѥ ‫΂̂װ‬ǱÏՎÏՎŴ‫ױ‬
P-37 ‫ש‬ӑ Aspergillus oryzae g»ÇÇÌփĀԠb¦ÉʊуǮϨ Ŧϥ, Ǳƒ jG|, ͎̤ ʡÒ‫װ‬ՎЗ͓͜ ‫׏‬ѼЗ‫ױ‬
P-38 Roles of AoLAH in regulating Woronin body position and function in Aspergillus oryzae
Pei HAN, Feng Jie JIN, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., The Univ. of Tokyo)- 8 —

P-39 ¯Ì±eFM|ǭ̫ DNA gÆÌ¾Ȫŀ͔ɩgӽ̰֓Ϩйɗ‫ײ‬ϨÜФգ‫ײ‬ϱȹ̊ ‫װ‬ǘϚǱÏϟÏղĈ‫ױ‬P-40 ‫ש‬ӑ A. oryzae eFM|Ð¦³Ð֛ՠ¤ÊÐÈÈÈ¦ÆÌ³ÆÐ
AoAtg26 g͜Ҧӽ̰Ӑ֚֩ʉ‫ײ‬Ϩʩ֩á‫ײ‬ŵ̤ŲjO ‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬P-41 tWÒ  Óu |}u} u}~u}uv   e !H6¦A R ©¦+ o
k!1Yxu < xu i2xu &l ‫ײ‬ǱщĤÒu Òk:' Ð»ÃÊGÓ

P-42 ‫ש‬ӑϩ̫˶Ӹ MOA ÉÐ‫מ‬Ͽϝ̼gĕӱbÊÌփϥϦҦgӽ̰ȹ̤йå‫ײ‬ȫŐʒҍϟ‫͉ײ‬ź̂‫ײ‬нΒԸƢ‫ײ‬Ǳа̢̫‫ײ‬ʉʹĺô‫ײ‬ŧӟְǨ ‫װ‬ƜǔǱÏՎ‫ױ‬P-43 јϒӑ Trichoderma reesei eFM|ӑĒǭ҇ѸϒϏԺƙʣցѣgˍѤϨƆċʓ 1‫˶ײ‬ϨҍԲȆ 2‫ײ‬ʉϨΌú 1‫ײ‬ǙɅТϥ 3‫̦ײ‬ȹΜƗ 3‫ײ‬Ǳ֨͢Ȇ 4‫ײ‬ȫоƆΡ 1
‫װ‬1 ֗ȻʮЧǱÏϥϏ‫ײ‬2 ЧȋʮӨ˄Һ͓͜‫ײ‬3 õռǱȋÏϥȉǇЗгʩ‫ײ‬4 ѷƙϭķЗгˢ˖‫ױ‬P-44 јϒӑg Poly(ADP-ribose) glycohydrolase gˍѤƊkXgĶI
ɗֈζ 1‫׆ײ‬ӟҍб 1‫̵ײ‬ȱĢú 2‫מײ‬ԦЊ͚ 2‫ײ‬ʉʹĺô 1‫ײ‬ŧӟְǨ 1
‫װ‬1 ƜǔǱÏՎ‫ײ‬2 тΈǱ֤ÏϥƨϣǢ‫ױ‬P-45 ‫ש‬ӑ Aspergillus oryzae ӑĒŉ֊ȷµÊ¤Ð ADAM g͜Ҧӽ̰ȫ̱ʻƸ‫ײ‬ŜϨΓ, нŉժ֯, ȹɳΌɗ ‫̭װ‬ՎɈǱ֤ÏʊϥŴ‫ױ‬P-46 ‫ש‬ӑHϦϥU| hydrophobin RolA bǄĒӪ‫֚׀‬gЋîĕϧe֛U|ЗгϨÜʻ̢ 1‫ײ‬Ϩշɬͮ 1‫͛מײ‬ʅ 2‫ײ‬ō͙Բʣ 3‫̚ײ‬ϨЭɴ 4‫֡ײ‬պ˭ʕ 1,2‫װ‬1 ̭ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ײ‬2 ̭ŵǱÏ̢̫З‫ײ‬3 ȹɳǱϟÏϏԺϥƨŴȋ‫ײ‬4 ̭ŵǱÏǮĺЗ‫ױ‬
P-47 ‫ש‬ӑ¸µ¡ÌֿʜƐʏµÊ¤ÐgցѣȋЃʏԺgӽ̰нŉЎϟө8Ȼ̤ѻȆ8֡ģ̅Ӂ8ŜϨΓ8ȹɳΌɗ8нŉժ֯ ‫װ‬ՎɈǱ֤ÏʊϥŴ‫ױ‬
P-48 3D À¥ÇÌŗϧSZʲϥϏԺÆÐgǫϴĒԄԀÜȹƩͮ‫ײ‬ȹϨְ÷‫ײ‬ӴϨΌɍ‫ײ‬МԩΊɬ ‫װ‬ȞȹЍǱÏϥɈ‫ױ‬P-49 ‫ש‬ӑgŎçցѣg͜Ҧӽ̰Ϩà ֧Ȇ‫ײ‬Ȼ̤ ѻȆ‫ײ‬ŜϨ Γ‫ײ‬ȹɳ Όɗ‫ײ‬нŉ ժ֯ ‫װ‬ՎɈǱ֤ÏʊϥŴ‫ױ‬P-50 Aureobasidium pullulans eϩ̫U|Ȏʑ‫ח‬փ-4-ʹփŴցѣղĈȆgӽ̰̭ϨДΌ‫ײ‬ǳϨÒҾ ‫װ‬ȖɁǱՎÏʊϥЧ‫ױ‬P-51 Aspergillus nidulans g GfsA h O-ÇÌg Galƒ ƙʣU|ѕՆЫցѣaB|
ĺ̮ծ 1‫ײ‬íСΊǗ 2‫ײ‬ΕֈǍՊ 1‫ײ‬нɅӝ 2‫ײ‬ɼӟ͢ŗ 2‫ײ‬ֈ̨ƴə 1‫ײ‬Ȼʻí 1
‫װ‬1 ɀǔǱȋÏϥϏϥƨÏʊʂɈ82 äǱÏ֤ÏՎ‫ױ‬P-52 °ÆgÇ»¨ÐղĈȆg͜Ҧӽ̰ȫ̱Ǳԃ‫ײ‬ȫ‫׬‬ĭǳ‫ײ‬Ϩ‫׮‬ӯí ‫֗װ‬Ȼ‫מ‬ȥÏϏԺɈ‫ױ‬- 9 —

P-53 ½¬ÇΎʏƊkǼǡʏОU‫ש‬ӑ Aspergillus oryzae g D-½¬փϐϴЃ½¬¸µ¡
Ð(DamA)gӽ̰̮Ö‫͉װ‬Ϩ‫ױ‬Ўϩҍ 1‫ײ‬ÜɅƀá 1‫ײ‬ǮϨŦϥ 1‫ײ‬ÝðΜÒ̝ 1, 5‫̡֋ײ‬Ҝ 1‫̜ײ‬պ‫ׇ‬ǳ 1‫ײ‬ǲֈø 2‫ײ‬ЖϨƀ͚ 3‫ײ‬ȹɳΌɗ 4‫ײ‬нŉժ֯ 4‫̤͎ײ‬ʡÒ 1‫װ‬1 ՎЗ͓͜Ï‫׏‬ѼЗ‫ײ‬2 ǲֈÌ¾‫ײ‬3 ̙́Ō‫ײ‬4 ̭õՎɈǱÏ֤‫ײ‬5JIRCAS‫ױ‬

P-54 ‫ש‬ӑ Aspergillus oryzae pepO ղĈȆ͞˗̼gӑĒǭÌ§ǒµÊ¤Ðe^C`ÕֈѯȆ‫ײ‬ŜϨΓ‫ײ‬ȹɳΌɗ‫ײ‬нŉժ֯‫װ‬ՎɈǱ֤ÏՎ‫ױ‬
P-55 Aspergillus oryzae gӑĒŉ֊ȷµÊ¤Ð saccharolysin homolog g͜Ҧӽ̰ЖɅ ֯Ò̝‫ײ‬ŜϨ Γ‫ײ‬нŉ ժ֯‫ײ‬ȹɳ Όɗ ( ՎɈǱ֤ÏՎ )P-56 Trichoderma reesei eȉǌU| 10 Ю‫׌‬gβ-glucosidase isozyme gցѣŴȋЃʏԺջ ƀΌ‫ײ‬ֈ‫׮‬ŦÒ‫ʹײ‬ֈ͢Γ‫ײ‬ǲֈҾɴ‫װ‬ĤɆǱ֤Ïϥƨ͜ҦÏ³®ÐɈȋ‫ױ‬P-57 ¼£g³©ÈÆ©ÌÌÀ©ÇÐղĈȆg͓՟b͜Ҧgӽ̰ȖɅ‫ײ÷ך‬ŲϨȭ͚‫ײ‬Ϩ‫׮‬ӯí ‫֗װ‬Ȼ‫מ‬ȥÏϏԺɈ‫ױ‬
P-58 ‫ש‬ӑ hydrophobin RolA b cutinase CutL1 ֚gЋîĕϧe֛ØU| RolA gǡǗʏͩǗȩ‫ ט‬ӯԔ 1, Đӟ ǱԲ 1, ֊ Ĺƅ 1, ̨Ǔ Ńӆ 1, ÕƆ ĭí 1, ‫ ͛מ‬ʅ 2, ȹɳ Όɗ 3, ֡պ ˭ʕ 1
‫װ‬1 ̭ŵǱ֤ÏϥϏϦ͏ţʣ‫ײ‬2 ̭ŵǱÏ̢̫З‫ײ‬3 ̭õՎɈǱÏʊϧϥϏЧȋ‫ױ‬P-59 ‫שק‬gӱ՟թЬeFM|Ё‫ש‬ӑg¦ÆÌÇµ¦Ð¾ӽ̰
íСΊǗ 1‫͉ײ‬Ò͚ 1, ƩϨ͢ǳչ 2, ͈Ɔɦƀ 2, ‫מ‬ÖФ̅ 2, Ǳ͉Ģչ 2, ϨĀɦú, 1 àƆƬ 1, ɼӟ͢ŗ 1‫װ‬1 äǱ֤ÏՎ, 2 ÔƩտ‫ױ׌‬
P-60 ‫־‬ҋǖ‫ה‬љey|ЁҿҬ̥ʹȆӑ Phanerochaete chrysosporium gÆÌʣőʊуe֛U|ЗгŲ̈Ƌњ‫ײ‬Ǚ ź̂‫ײ‬ïŹɃǍ˻Ȇ‫סײ‬ȿ͢Γ‫̭װ‬Ǳ֤ÏՎϥŴ‫ױ‬P-61 ‫ש‬ӑgÐºÌºÆ¦ʰŘ֛ՠƿȆ CreAÏCreB íևИǦey|½ÆÐ‫מ‬ϥϦÒλ̈́Ȇ‫ײ‬ϨÜϡɌ‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå ‫̭װ‬ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ױ‬P-62 ÆÌԒȪǒ¿ÆՆŋƿȆ AmyR::XlnR ey|½ÆÐϥϦ֊Ȇĸɗ‫ ׶‬ϨÜȤǗ‫ ׷‬ÝðΜÒ̝‫ ׷‬ʉʹĺô‫ ׶‬ȫ̱ƬǴ‫ ׷‬ŧӟְǨ‫׶‬‫׶װ‬ƜǔǱ֤ÏՎ‫׷ײ‬ƜǱ֤ϥƨՎ‫ױ‬
P-63 Trichoderma reesei eFM|ÈÆÐϥϦe֛ØU|¦ÆÌ»ÐÐgӽ̰ԦƒǱ͚‫˻ײ‬ÖФӧ‫ײ‬ƓɅ֩њ‫ײ‬ʉϨΌú‫ײ‬ȫоƆΡ ‫֗װ‬ȻʮЧǱÏϥϏ‫ױ‬
P-64 Aspergillus nidulans ՆŋƿȆ ManS g͜ҦΥնñåȆ‫ּײ‬ȹ̢̫‫֊ײ‬ÝõȆ‫ ̨̡ײ‬Ў‫ײ‬ȫ̱ƬǴ ‫װ‬ƜǱ֤ϥƨՎÏϥϏ͓͜‫ױ‬
P-65 °ÆgÇ©ÌőӽցѣϿϝԙъ͓͜eFM|ÈÀ¥ÂÇÌgɷŢ̣ō‫מ‬ʉ 1‫ץ̤֞ײ‬ӧ 1‫ײ‬ʃ͵ТȊ 1‫̤ײ‬ϨØÒ 2‫΅ײ‬΍ɬđ 1‫̡֋ײ‬ÒȔ 1‫ײ‬ŀ͹ĢÒ 1(1 ίԴЍǱÏϣ‫ײ‬2 õǱÏՎ)- 10 —P-66 Aspergillus aculeatus ÈÊÐљ®

¼őӽցѣղĈȆgϿϝŘɿƿȆgƛȒ

զéҒǳ‫ײ‬ǆͣѲҍ‫ײ‬Ԧĥ΂‫ײ‬οԦ‫ׅ‬Ò‫ײ‬ɅƒŝƗ‫֞װ‬ɡǱ֤ÏϥϣЧ‫ױ‬P-67 Expression cluster analysis of the transcriptomes at sequential growth stages in the white-rot fungus,
Phanerochaete carnosa, grown on aspen and spruce woods.
Hitoshi Suzuki (RIKEN, CSRS), Philip Wong, Chi-Yip Ho, Yunchen Gong, Kin Chan, Elisabeth Tillier andEmma Master (Univ. of Toronto)
P-68 ‫ש‬ӑgՆŋƿȆ AmyR b MalR gғԆɼĥ‫ד‬bȎȒʏ֋̡дǳ‫ײ‬ϨÜϡɌ‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå ‫̭װ‬ŵǱ֤ÏՎÏϥϏϦ͏ţʣ‫ױ‬P-69 pH ʊуՆŋƿȆ PacC ey|јϒӑÈÆÐղĈȆϿϝŘɿǆͣѲҍ 1‫ײ‬ӕƆǱР 2‫ײ‬Ṳ̑ĭǳչ 1‫֊ײ‬ÝõȆ 1‫̨̡ײ‬Ў 1‫ײ‬ȫ̱ƬǴ 1‫װ‬1 ƜǱ֤ϥƨՎÏϥϏ͓͜‫ײ‬2 źӗǱÏЎӑÌÐ‫ױ‬P-70 ‫ר‬ӑ xyrA Ɗk larA ղĈȆg XlnR b AraR ey|ŽԙЃϿϝŘɿЖɅ‫ח‬ž‫ײ‬Ʌλ̏ҍ‫ײ‬ֈƒđƗ‫֊ײ‬ÝõȆ‫̨̡ײ‬Ў‫ײ‬ȫ̱ƬǴ ‫װ‬ƜǱ֤ϥƨՎÏϥϏ͓͜‫ױ‬
P-71 Aspergillus aculeatus Ê±ÐʊуƿȆ ClbR bXg¯ÆÊƿȆ ClbR2 g ĕϧ͜ɟӽ̰Ʌ̨ɵß‫ײ‬ǆͣѲҍ‫ײ‬Ԧĥ΂‫ײ‬οԦ‫ׅ‬Ò‫ײ‬ɅƒŝƗ‫֞װ‬ɡǱ֤ÏϥϣЧ‫ױ‬P-72 Molecular basis that divides ManR-dependent and ManR/McmA-dependent genes in Aspergillus
nidulans
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Grad. Sch. Bioagric. Sci., Nagoya Univ.)
P-73 јϒӑ Aspergillus niger ϩ̫ PKS-NRPS ղĈȆÆÐgɰŘϿϝΚɅȊҏ‫͍ײ‬ȫ8Ҩ̤˨ə8֡պո̝ ‫̭װ‬Ǳ֤Ӟ‫ױ‬
P-74 ¦Ç¤ÌϥƙʣղĈȆgՆŋƿȆÐ§U| Tri6 g͜Ҧӽ̰ÜɄ đÒ‫ ׈נײ‬Ҿə‫ײ‬ŜϨ Òӧ‫ײ‬ɏɅ ִĀ‫ײ‬ȫ̱ ƬǴ‫ ̨̡ײ‬Ў ‫װ‬ƜǱ֤ÏϥƨՎ‫ױ‬P-75 GkͰ¤È¸Ì§ÐÈgϥϦŘɿ¿©¾
̤ȹ‫מ‬ə‫֗ײ‬Ϩӯá‫װ‬ϟЗÏʲϥϏԺ‫ױ‬
P-76«Cv\ϺӑeFM|¤ªÌփgϥϦŘɿ¿©¾ƊkϥϟΎʏȯʋ֍‫̤ײ‬ȹ‫מ‬ə‫ײ‬į́Ƌƕ8֗Ϩӯá ‫װ‬ϟЗÏʲϥϏԺ‫ױ‬P-77 ˶Ӹí͟ĀԠŘɿƿȆbS`g Aospt3 g͜Ҧӽ̰ɧλְ÷ 1,2‫΀ײ‬ŉԥá 1,2‫ײ‬ȼÖƩӯ 1,2 ‫װ‬1 ɜȿǱ֤ÏļлЗ‫ײ‬2 տѼЗ‫ױ‬
P-78 Emericella variecolor eFM|¤È¸Ìƙʣցѣg ˍѤÔ͛֩к‫̮ײ‬ϨĚǱ‫ײ‬ΚɅȊҏ‫֡ײ‬պո̝ ‫̭װ‬Ǳ֤ÏӞ‫ױ‬- 11 —P-79 јϒӑϩ̫¿Ê¤È¸¬§ anditomin gϥƙʣЗг̮ϨĚǱ‫ײ‬ΚɅȊҏ‫֡ײ‬պո̝ ‫̭װ‬Ǳ֤ÏӞ‫ױ‬P-80 Aspergillus fumigatus ϩ̫ pseurotin A ϥƙʣ͓͜gӽ̂Ǫ̈ɦÒչ‫̮ײ‬ϨĚǱ8ΚɅȊҏ8֡պո̝ ‫̭װ‬Ǳ֤Ӟ‫ױ‬P-81 јϒӑ Fusarium sp. FN080326 ̼ϩ̫³Ç¡Ì A ϥƙʣղĈȆÆÐgƛȒŧӟЊ͚ 1‫ײ‬Jun-Pil JANG2‫ײ‬Jae-Hyuk JANG2‫͛מײ‬Ģí 1‫ײ‬Jong Seog AHN2‫֗ײ‬Ϩӯá 1
‫װ‬1 ϟЗÏCSRS‫ײ‬2KRIBB‫ױ‬
P-82 јϒӑ Fusarium sp. RK97-94 gÈÆÌϥƙʣղĈȆÆÐÜg¿¡ÈǗՆЫցѣղĈȆ luc1 b¦ÆÌ»ÐÐղĈȆ luc4 g͜Ҧӽ̰ŧӟҒ 1,2, ̤ȹ‫מ‬ə 1, ɧϨΌ 1, ֓Ħ‫מ‬ʉ 2, ֗Ϩӯá 2 ‫װ‬1 ϟЗÏʲϥϏԺ‫ײ‬2 ̭õϟЧǱ‫ױ‬
P-83 ‫ש‬ӑ hstD ИǦey|í͟ĀԠϏϥϦљgΎʏŴ΀ŉԥá 1‫ ײ‬2‫ײ‬ԧΒŗ̳Ȇ 2‫ײ‬ȼÖƩӯ 1‫ ײ‬2 (1 ɜȿǱ֤ÏļлЗ‫ײ‬2 տѼЗ)
P-84 ‫ש‬ӑ Aspergillus oryzae ϩ̫µ III ǒ»Ç§ƙʣցѣ CsyB ey| 3-acetyl-6-alkyl-

α-pyrone gϥʣ͛ĺ Ԕ 18‫͛מ‬Ȑ̂ 18‫מ‬ǉ ȗ 18‫׆‬ϨźȨ 18ŵ̤ŲjO 28ӟð ų 1‫װ‬ȼʪŸЧǱÏӞ8̭Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬P-85 DNA í̤֔Œ˴ey|
1«Cv\Ϻӑg͖ЃղĈȆˣǫ·1ϧáÝƬå ؉ ӌΞԲá ؉ ǱֆĥÒ 1؉̚͹ť 2؉ ̓ϨӇ 1 (1 ̂΂Ǳ֤ՎÏ2 ՎɈǱ֤Վ)P-86 ЎӑϧCZ Roxithromycin g˶ӸĕϧπgˍѤχǏӈđ‫ײ‬Жð̎‫ײ‬ѡ՛ċ΂‫ײ‬ĦˀəƗ‫ײ‬ӏƆíÔϫ‫ײ‬ǸҾ‫װ‬ʣɅ‫ױ‬ʒ‫֓ײ‬Ħ‫מ‬ʉ‫̭װ‬ϟǱ֤ϟɈÏʊϥЧ‫ױ‬P-87 ¦ÀÊPrӗ̶ϺӑeFM| cAMP ¨ÈĈիѫՂ֛ՠղĈȆg͜Ҧӽ̰ΨԦ̏‫ײ‬ďϨżå, ŵŐ֯ϥ‫΅ײ‬΍ɬđ‫ײ‬ϨÜźȨ‫װ‬õǱÏ֤ÏՎ‫ױ‬
P-88 Aspergillus fumigatus gϺƆʏe֛|ƿȆg͜Ҧӽ̰տð‫ח‬Ǹ͹‫ײ‬ǱӌϨѣȆ, ï¬ð՛ ‫װ‬źӗǱÏЎӑÌÐ‫ױ‬P-89 ϺƆЎӑ A. fumigatus ɮͰŴU|¼
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P-90 Cv\Ϻӑg¶¤Ê 3 ։Ē G Ì¯Ժ(´Ã©¢¦gӽ̰‫־֚׍‬Ӂ 1, 2‫ײ‬ɅɁȤ 1‫ײ‬Ӵ̨‫ת‬ֆ͹ 2‫װ‬1 ֹǱ֤ÏϏԺɈȋ‫ײ‬2 ϥϏЗ‫ױ‬- 12 —P-91 ¦ÀÊPrӗ̶Ϻӑg ChSte20 Fyk ChCla4 ղĈȆg͜Ҧӽ̰ŵŐ֯ϥ 1‫΅ײ‬΍ɬđ 2‫ײ‬ďϨżå 1‫ײ‬ΨԦ̏ 1‫ײ‬ϨÜźȨ 1 ‫װ‬1 õǱÏ֤ÏՎ82 ίԴЍǱÏϣǢЧȋ‫ױ‬
P-92 Ç‫׌‬οϸϺӑeFM| Ras GTPase ΎʏŴÌ¯Ժ CoIra1 h CoRas2 úS` cAMP ¨ÈĈիѫՂb CoMekk1-Cmk1 MAPK ѫՂgÕΏŘɿƿȆbS`֛ØU|ƆϨ Ը‫ײ‬àģɦá ‫װ‬õɡǱ֤Ïϥϣ‫ױ‬
P-93«Cv\Ϻӑ¼

È MoCV1 b MoCV1-B g¬¾͓՟gͱՈ‫ן‬ðճȆ‫ײ‬ΒȹĢÒ‫ײ‬ՖϨѠƕ‫ײ‬ŧӟĸ‫̨̡ײ‬ĸֆ‫ײ‬УƆ˨ӧ‫̚ײ‬͹ť‫ײ‬ȢȻʅ‫͉ײ‬ȹӯĻ(ՎɈǱ֤ÏՎ)
P-94 ͋ϏϺƆʏ Alternaria alternata eFM| global regulator LaeA g͜Ҧӽ̰‫מ‬ȱƩȔ 1‫ײ‬Իֽ̡Ň 1‫̤˞ײ‬Ė̂ 2‫ײ‬ЖƆô 1‫̹͋ײ‬ȭʉ 2‫ֶײ‬Έ̻í 3‫ײ‬ĿϚǗÒ̝ 1‫װ‬1 ‫ף‬ƏǱÏ2 ƜǱ֤ϥՎÏ3 ‫ף‬ǱŸ‫ױ‬
P-95 μҿGkϺӑgÐ¦³Ð֛ՠղĈȆ BcAtg1 g͜Ҧӽ̰ďϨżå 1‫΅ײ‬΍ɬđ 2‫ײ‬ϨÜźȨ 1 ‫װ‬1 õǱÏ֤ÏՎ‫ײ‬2 ίԴЍǱÏϣǢЧȋ‫ױ‬P-96 Ç‫׌‬οϸϺӑeFM|Őӄցͯ RAM «¢¦ËÐ͓ʣƿȆ CoPag1 h͋ϏϐϴЃ¨ÈƐȚúSZþАƺɳʣe֛ØU|ȫϚџĀ‫ײ‬Ǐƒ ͤ‫ײ‬àģɦá ‫װ‬õռɡǱ֤Ïϥϣ‫ױ‬
P-97 ¦¼¦ÈÐ¨ÇӉ̶ϺӑHģ̚U|ϺƆʏ̸ҿĒg͓՟ӽ̰Իֽ̡Ň 1‫ײ‬ǮԴ͢ъ 2‫̹͋ײ‬ȭʉ 3‫ֶײ‬Έ̻í 4‫ײ‬ĿϚǗÒ̝ 1‫װ‬1 ‫ף‬ƏǱՎÏ2 ȻȹǱϟÏ3 ƜǱ֤ϥՎÏ4 ‫ף‬ƏŸ‫ױ‬
P-98 µ¡ÌůʝŘɿU|Őӄցͯg RTS1 g¹ÀÊ CoRTS1 hÇ‫׌‬οϸϺӑeFC`կŒdҥȆɳʣ8þАƺɳʝɳʣeʇӵaB|
Ʌл ˾, ΛϨƕҍ, Ǐƒ ͤ, àģɦá ‫װ‬õɡǱ֤Ïϥϣ‫ױ‬
P-99 ½¦Ì§Çȳǌǒg Enoyl-CoA hydratase hÇ‫׌‬οϸϺӑgϺƆʏϿϝeʇ‫׆‬aB|
΅΍ɬđ‫ײ‬Ǯ:Ҿɦ‫ײח‬ȫ̮‫͘ײ҉ח‬ȹѻ‫ײ‬ԦƒʻГ‫ײ‬Ýȹ‫ת‬ҍ‫ײ‬ŀ͹ĢÒ‫̡֋ײ‬ÒȔ‫װ‬ίԴЍǱÏϣ‫ױ‬P-100 Ç‫׌‬οϸϺӑgˏȒѧҥ͐ʏƿȆ CoTEA4 Fyk CoMOD5 g͜Ҧӽ̰΀Öҍռֆ‫ײ‬Ǐƒ ͤ‫ײ‬àģɦá‫ײ‬Ր ĺ÷ ‫װ‬õɡǱ֤ϥϣ‫ױ‬- 13 —







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糸状菌における菌体外消化システム：分解酵素の分子機構から見えてくる
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Igarashi, K. et al. Fung. Genet. Biol. (1997)
Igarashi, K. et al. Eur. J. Biochem. (1998)
Igarashi, K. et al. J. Biol. Chem. (1999)
Igarashi, K. et al. Biochem. J. (2002)
Igarashi, K. et al. FEBS J. (2005)
Igarashi, K. et al. FEBS J. (2006)
Igarashi, K. et al. FEBS J. (2007)
Igarashi, K. et al. J. Biol. Chem. (2009)
Igarashi, K., Uchihashi, T. et al. Science (2011)

Extracellular digesting-system in filamentous fungi: Fungal strategy for the biomass
utilization “visualized” from molecular mechanisms of degrading enzymes
Kiyohiko Igarashi(Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The
University of Tokyo)- 15 —







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[2] Seifert et al. 2011. The genera of hyphomycetes. CBS biodiversity series, vol. 9 (ISBN978-90-70351-85-4; http://www.generaofhyphomycetes.org/).
[3] Morris and Finley. 1967. Am Midl Nat 77: 200–204.
[4] Diederich et al. 2008. Sauteria 15: 205–214.
[5] An et al. 2012. Fung Biol 116: 1134–1145.

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3,4

References
1: Ishidoh K et al 2013. Curr. Genet in press
2: Shimizu T et al 2005. Appl Environ Microbiol 71:3453-3457.
3: Sakai K et al 2009. Biotechnol Lett. 31:1911-6
4: Sakai K et al 2012 Appl Microbiol Biotechnol. 93:2011-22.

- 28 —







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Masato Hirose , Moriyuki Kawauchi, Kazuhiro Iwashita (Hiroshima Univ, NRIB)

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Alternation of biosynthetic regulation and its effect on epigenetic states in Chaetomium globosum.
Takehito Nakazawa, Kan’ichiro Ishiuchi, Yasutaka Gotanda, Hiroshi Noguchi, Kenji Watanabe.
(School of Pharmaceutical Sciences, University of Shizuoka)- 30 —O-5 (P-96)
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Colletotrichum orbiculare CoPag1, a component of RAM network in Saccharomyces cerevisiae, is involved inappressorium development triggered by plant-derived signals
Sayo Kodama, Ayumu Sakaguchi, Yasuyuki Kubo
(Graduate School of Life and Environmental Sciences, Kyoto Prefectural Univ.)

O-6 (P-63)
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Hiroki Taniguchi, Hideyuki Kusaka, Tkanori Furukawa, Yosuke Shida, Wataru Ogasawara(Nagaoka Univ. of Tech)- 31 —O-7 (P-56)
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Silai Zhang, Akihiko Ban, Naoki Ebara, Osamu Mizutani1), Mizuki Tanaka,TakahiroShintani, KatsuyaGomi(Grad.Sch,Agric.Sci.,Tohoku Univ., NRIB1))- 32 —O-9 (P-43)
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Exploration of protein related to cell surface fibrous material synthesis of Trichoderma reesei
Shingo Tahara1, Mikiko Nitta2, Yosuke Shida1, Yoshiki Horikawa3, Junji Sugiyama3, Masako Osumi4, Wataru
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Investigation of fungal SSH-libraries for biomass decomposition
Sonyon Ann, Akihiro Oguchi, Natsuko Ichikawa, Tomohiro Sekigawa, Kei Kamino(NITE)

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Production of useful materials from fungi with blue light stimulation
Masanobu Kojima1, Ryuhei Miura1, Satoshi Mihara2, Masaki Ichimura2(1Grad. Sch. Agri., Shinshu Univ.; 2JA Nakanoshi)- 34 —O-13
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A. oryzae ¿ ÌbU|љѱ, A. flavus ¿ ÌbU|љѱeő‫׌‬Q}ZHŁ`gљѱeFC`Ú̼HΝ
ǌSZZu, ÚЮȏŁeŷŖU|ehy{Ԏѧdӽ̰ӧDʇӵHB|bҖEz}Z9XOaɜыaī{gdCӽ̰bd|yD ISSR љѱѬ̲eǗ_I A. flavus ӑ̼ձʳS‫ר‬ӑ DNAchip ϧC`¬¾Éӽ̰ӧ]Z9A. oryzae gƘљѱ 13 ̼bùƾɾz}Z A. flavus g¥ÐĘϧS Gene spring e`љѱӽ̰ӧ]ZbO~Úӑ̼ҎĥЛeőG}Z9āÕgìGz A. flavus ӑ̼Ҏb A. oryzae ӑ̼Ҏ̂БeŷŖƔҦbd]Z9
Genome wide phylogenetic analysis of Aspergillus flavus and A. oryzae
Tamy Ohota, Ken Oda, Kazuhiro Iwashita, Nami Goto (NRIB)

O-14©¦Ê¡«ÌgϿӷgXg  ҘʏŴ͓͜gӽ̂ƥŲ˨‫פײ‬СȤ̇, ̵ȱĢú, ‫מ‬ԦЊ͚‫װ‬тΈǱ֤ÏϥƨϣǢ‫ױ‬ʂϥϏgÒփŴзѣ‫װ‬NO‫ױ‬eȣU|ҘʏŴe^C`h8ŸȋÏՎȋőֈa‫מ‬C֛ʆˀZ}`C|v
gg8ѧӑbցͯȣԨbSZЗгHÞaB{8јϒӑgX}e֛U|Згh͐u`ȬdC9̤Згah8јϒӑg˶Ӹd NO ҘʏŴղĈȆbXgĕϧ͓͜ӽ̂SZga8O}ǝƤU|9
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dC9
Discovery of an NO-resistant peptide nitrosothionein from Aspergillus nidulans.
Shengmin Zhou, Toshiaki Narukami, Shunsuke Masuo, Naoki Takaya(Graduate School of Life and Environmental Sciences, Univ. of Tsukuba)- 35 —O-15¼

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ӷŐSZ9OgղĈȆh‫ײ‬Ȇƻӑ Bipolaris oryzae a¿Æ©ÌϥƙʣƗ|ՆŋƿȆ BMR1 g¹ÀÊaB]Z9¼ av Gf.BMR1 H¿Æ©Ìƙʣe֛ØS`C|bҖEZH‫ּײ‬ҿĽÖagϿϝ։hՔѥǭĽÖgX}bǱɋHdG]ZObGz‫ײ‬Gf.BMR1 eŧE`ýgƿȆg֛ØHОƮQ}Z9CV}eWy‫ ¼ײ‬hּҿĽbՔѥǭĽӷőM‫ײ‬XgɋϴŗϧU|ϥϟЃ͜ɟgȉǌHОƮQ}Zga‫ײ‬ùɼh Gf.BMR1 ǗπeXgȉǌ̂zGeS`CIZC9
Grifola frondosa distinguishes between blue light and near-ultraviolet light.
Atsushi Kurahashi1, Shimoda Takafumi1, Masayuki Sato1,2,3, Fujimori Fumihiro2,3, Jyuniji Hirama2, Kozo Nishibori1(1Yukiguni Maitake, 2 Hyphagenesis, 3Tokyo Kasei Univ. 4Kanazawa Inst. Tech. EOE)

O-16
Aspergillus fumigatus eFM|˶ӸϺƆƿȆgˍѤb͜Ҧӽ̰ձʴЃЁҿҬ̥ӑ Ceriporiopsis subvermispora g®©ÇÌeȣU|ѧҥʊуΥշɀ÷‫͉ײ‬ԸÒչ‫̡ײ‬ʦɵȆ‫֗ײ‬ԦɅ֩Ǳ‫ײ‬Ӵ̨ӯʉ‫̤ײ‬ϨØÒ‫ײ‬ΥՏ֩Ɨ ‫װ‬õǱÏϥȉЗ‫ױ‬ձʴЃЁҿҬ̥ӑ Ceriporiopsis subvermispora h‫ײ‬
=3ցѣѧҥǤŉeբŀQWVeÇ©Ì‫מ‬ձʴЃeőӽU|‫׿‬4̡̧Ҭ̥Ŕ̠e֗֔ҧҠփgőΆbթփŴΎϿeԽOU‫׿‬5Ç©Ì¸È ÐΎʏ̚SdC‫׿‬6ÈÊÐőӽgƆƿbd|ʹփŴÆÈʰŘљ̚U|‫׿‬7Ê±°§ÊÆÐΎʏ̚SdC>с‫ײ‬ЁҿҬ̥ӑgÀ¥ÈЮaB| Phanerochaete chrysosporium сbhϴd|ϐʄ
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subvermispora HcgyDdѧҥʊуОUGe^C`ԙoZ9®©ÇÌΞŧƊkֿΞŧgÈÐοѣΫbU|ΙĒƙʣǖǎe C. subvermispora X}Y}ǖ
‫ה‬SZbO~‫ײ‬ýgЁҿҬ̥ӑbhϴd{‫©®ײ‬ÇÌey|ϥҡ֠Șhӷz}dG]Z9rZ‫©®ײ‬ÇÌΞŧey]`ӑgϥҡƊkӑĒǭǮѕgǣŧbbveǖǎÜgÈÐ։HӘSKΤȬSZ9Qze‫ײ‬¯È½¡ÌփwÇ¬ÐÈփсg֗֔‫ג‬ƩÏ×‫ג‬ƩҧҠփw ceriporic acid‫װ‬³Ì¦ÌƍʊeFM|Ôĝg֌gյĺʰŘU|Oba‫ʹײ‬փŴÆÈgϿϥʰŘU|̤ӑϩ̫g˶ӸĀԠϏ‫ױ‬gϦϥ։HǣŧSZ9Ò˷‫ײ‬Suppression Subtractive Hybridization ·ey{®©ÇÌΞŧey]`ՆŋHԒȪQ}|ղĈȆgƏɾԊsZ9XgѬ̲‫©®ײ‬ÇÌсgӂ‫˹ח‬ŴƙϏgőӽwҧҠփgϥƙʣƊk×‫ג‬ƩŴe֛ØU|ցѣղĈȆсHƏɾaIZ9āÕy{‫©®ײ‬ÇÌсgÇ©Ìőӽ³Æ¿Ì¦h‫̧̡ײ‬Ҭ̥͓͜ŘɿU|¨ÈőȆgÒ^aB|ƔҦʏHОƮQ}Z9

Vanillin-induced cellular response in the selective white rot fungus, Ceriporiopsis subvermispora
Takahito Watanabe, Kenichiro Mori, Ayako Kido, Takahiro Hasegawa, Hiroshi Nishimura, Yoichi Honda, Takashi
Watanabe (RISH, Kyoto Univ.)- 36 —O-17‫ש‬ӑÉ¡Ì͔Ì¯Ժ CreD gҪÇÌփŴey|ÈÐʰŘgŘɿϨÜϡɌ‫ײ‬ɗ̤Ƭå‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå‫̭װ‬ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ױ‬
É¡Ì͔Ì¯Ժ CreD h, ÐºÌºÆ ¦ʰŘŘɿƿȆgÒ^aB{, Ã±¡ÌÇÐb͖ЃÌ¯Ժbg µÐbS`͜ҦU|bˏȒQ}`C|9ʤ:hO}rae, ‫ש‬ӑeF
C`ҪÃ±¡ÌŴցѣ CreB gИǦey{ÈÐʰŘHӽ֥Q}|Ob(Òλz, 2012), CreD H¼È¦Ð¯Ð½ÐgÈÐĜȉЃdőӽe֛|Ob(ɗ̤z, 2012)ǝƤS`IZH, CreD ey|ÈÐʰŘgŘɿ͓͜h×̂aB]Z9̤Згah CreD gÇÌփŴպčƛȒS, CreD ҪÇÌփŴgÈÐʰŘŘɿng֛Øӽ̰SZ9
creB b creD gíևИǦey{ creB ИǦ̼HОUÈÐʰŘgӽ֥HʰEz}ZObGz, CreD HÈÐʰŘgŘɿe֛]`C|ObHОƮQ}Z9CreD g¹ÀÊaB|Őӄցͯg Rod1 h, Snf1¨Ðey{ÇÌփŴQ}|ObGz, CreD ŉeȉǌU| 2 хʩg Snf1 ͖Ѓ͔ÇÌÆ©Ìeҋ˔SZ9XgѬ̲, Ìӽ̰a‫מ‬őȆĮg¨ÈHΘǶSZObGz, Og 2 хʩgÇÌHÇÌփŴQ}`C|ObHОQ}Z9creB ИǦ̼eFC` CreD gÇÌփŴպčÈ½Ìփeҋ˔SZ
bO~, ÈÐʰŘgӽ֥HʰEz}ZObGz, CreD gҪÇÌփŴHÈÐҪʰŘe֛ØS`
C|ObĤzGbd]Z9rZ, ŅľϷͼ֢·ey{ CreD b HECT Ã±¡ÌÇÐ HulA bgЋîĕϧԙoZѬ̲, ҪÇÌփŴQ}Z CreD gsH HulA bЋîĕϧU|ObHОƮQ}Z9‫̤װ‬ЗгhϥЗÌÐ= ¬·ÐÄÌţŐǗНЃЗгˏբì͏>gˢ˖ƐM`ӧ}Z9‫ױ‬Regulation of glucose repression through dephosphorylation of arrestin-like protein CreD in Aspergillus
oryzae.
Mizuki Tanaka, Tetsuya Hiramoto, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.)

O-18
Cv\ϺӑeFM|°¦ÌÇÌ¿¡È¦ÆÌ³ÆÐgɷŢ
Kieu Pham Thi Minh, ðÕҏƀ, Ba Van Vu, Quoc Bao Nguyen, ͺϨĭÒ, Üȵ˰ǐ ‫װ‬СʦǱÏՎ‫ױ‬«Ч͋ϏCv\Ϻӑhʜ̸թЬeFC`‫ײ‬Ͽӄч‫ײ‬þАƺ‫ײ‬ʜ̸ӑјbC]Z͔:dʜ̸ƺȑ͟:bɳʣS‫ײ‬ŤЃdɳʝǫŴӧD9OgyDd¨½¢dɳʝɳʣehǱӸ͗dղĈȆϿϝgǫŴHĊ]`C|ObHҖEz}‫ײ‬XgÊ¼¡Ì͓՟ǫŴey|ղĈȆϿϝŘɿ͓͜ehҺƦHˀZ}|9̤Згah‫¾ײ‬Cv\ϺӑeFM|°¦Ì¿¡ÈŴgɷŢeΉЉS‫¾¬ײ‬eȉǌU| 7 ħg°¦ÌÇÌ¿¡È¦ÆÌ³ÆÐ‫װ‬HMT‫ױ‬g͜Ҧӽ̰ԊsZ9O}z HMT ղĈȆgИǦ̼ah‫ײ‬ҥȆϿӄ‫ײ‬þАƺɳʣ‫ײ‬ҥȆɳʣ‫ײ‬ʜ̸ʏdce͔:dÉ·Èg͞˗HԐuz}Z9Üav MoHMT4 bƨƜQ}ZŐӄցͯ SET1 ղĈȆgÐÊgИǦ̼ah‫ײ‬þАƺɳʣϙHŤЃeĎÖS‫ײ‬țÞaB|¾eFM|ʜ̸ʏȏŁeǶ]`CZ9Ì´Ê¢¤Ìӽ̰ey{‫ײ‬MoHMT4 h H3K4 e
FM|¿¡ÈŴʹDObĤzGed]Z9H3K4me2 ʲĒϧCZ ChIP-seq ӽ̰gѬ̲‫ײ‬Cv\Ϻӑ
ah H3K4me2 hÞbS`Ð¥Ì‫ׇ‬ǕeӚвS`C|ObĤzGbd]Z9rZ8RNA-seq ey|ղĈȆϿϝӽ̰gѬ̲‫ײ‬MoHMT4 ИǦ̼ah‫ה̻ײ‬ӑјa՝ɓϿϝHʰŘQ}`C|ĎϿϝղĈȆҎgϿϝHÕ̀S`C|ĵƝHB]Z9XgÒ˷‫ײ‬ֈϥ̼ahþАƺɳʣсeĊ]`ϿϝQ}|ղĈȆҎgϿϝÕ̀H‫ײ‬MoHMT4 ИǦ̼ah‫ײ‬ʰŘQ}`C|ĵƝHB{‫ײ‬MoHMT4 ey|°¦Ìĥ‫ד‬hղĈȆgϿϝԒȪbʰŘgÚ˷e֛ØS`C|ƔҦʏHОQ}Z9

Histone lysine methyltransferases are involved in various aspects of pathogenesis in Magnaporthe oryzae.
Kieu Pham Thi Minh, Yoshihiro Inoue, Ba Van Vu, Quoc Bao Nguyen, Ken-ichi Ikeda, Hitoshi Nakayashiki.
(Fac. of Agri., Kobe Univ.)- 37 —O-19ϺƆЎӑ Aspergillus fumigatus gÐÈŞҘʏeFM| AtrR gǮ͔d͜ҦӕƆǱР 1‫ײ‬ǱǞͤ 2‫ײ‬ΟʹŃʃ 1‫ײ‬Ʌ̤բ 1‫ײ‬ïƦŲå 2‫װ‬1 źӗǱÏЎӑÌÐ‫ײ‬2 ̭ŵǱ֤ÏՎ‫ױ‬ Aspergillus fumigatus h¸ÈÈϻgÞӵdƆƿӑa‫ײ‬ľϷ͜ҦgĎÖSZțÞeʜ̸U|9ӞŞgձʴҟhȬdKÐÈŞhևӵdɷŢ̲ZS`C|H‫̠֗ײ‬eZ|ʱӞhʜ̸ӑgҘʏŴʽI̾΂eֶ΢U|ÐHӷz}|9Qze‫ײ‬Վ͏őֈaĘϧQ}|ÐÈљՎӞey{ϣǢÜaÐÈҘʏϗɾSZ̤ӑey|ʜ̸v͠ɆŔubS`ӲˮgǎǕGzǝƤQ}`F{‫ײ‬ҘʏŴőȆ͓͜gŁȚӽ̂b˽ʎdȣфHͶuz}`C|9 Ŝɘrae‫ײ‬A. fumigatus eFC`ՆŋƿȆ AtrR gղĈȆИǦHƘЮÐÈŞeȣU|ʜƐʏ‫מ‬u‫ײ‬͖ЃѫՂaB|È¤ÊÐÈƙʣѫՂƿȆgϿϝĎÖɫIԽOUObОS`IZ9̤ϿӪah‫ײ‬ɲѫՂŘɿU|ObHДz}`C| bHLH ǒՆŋƿȆ SrbA gղĈȆИǦ̼ĕӱS‫ײ‬ƘЮӪϝǒgͱՈӧ]Z9XgѬ̲‫ײ‬ÐÈŞʜƐʏFyk‫ײ‬È¤ÊÐÈƙʣѫՂgÞӵƿȆ(cyp51A, erg3,
erg24A, erg25A)gϿϝĎÖh‫ײ‬atrR Fyk srbA ИǦ̼aƛЬɢeӻȡQ}Z9rZ‫ײ‬atrR ИǦ̼h srbAИǦ̼bƛ͔eĎփѣϣǢÖaϥҡaIdCObv̂zGeSZ9O}zgѬ̲Gz‫ײ‬ÚƿȆHŽԙЃeÈ¤ÊÐÈѫՂŘɿS`ÐÈҘʏe֛ØS`C|ObHҖEz}|9 Քɘ‫ײ‬ABC ¦ÆÌ»ÐÐAbcC/CdrB gղĈȆϿϝHòբS`C|ÐÈŞҘʏӑHǝƤQ}Z9‫׀‬ЁCObe‫ײ‬ֈϥ̼Fyk srbA ИǦ̼aӷz}|ÐÈŞeȣU| abcC/cdrB gϿϝʊуh atrR ИǦ̼aӷz}dG]Z9Qze abcC/cdrB ղĈȆИǦ̼HÐÈʜƐʏОSZObGz‫ײ‬AtrR hÈ¤ÊÐÈѫՂgŘɿeŧE‫ײ‬ABC ¦ÆÌ»ÐÐgŘɿGzvÐÈҘʏe֛ØS`C|Ob
HОƮQ}Z9
AtrR plays a crucial role in azole-resistance in Aspergillus fumigatus
Daisuke Hagiwara1‫ײ‬Ayumi Ohba2‫ײ‬Kiminori Shimizu1‫ײ‬Susumu Kawamoto1‫ײ‬Katsuya Gomi2‫װ‬1MMRC, Chiba Univ.‫ײ‬2Grad. Sch. of Agric. Sci., Tohoku Univ.‫ױ‬

O-20
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Hiroki Irieda, Hitomi Maeda and Yoshitaka Takano(Grad. School of Agriculture, Kyoto Univ.)- 38 —







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Mayumi Nakayama1,2, Kentaro Furukawa3, Akira Yoshimi1, Keietsu Abe1,2(1NICHe., Tohoku Univ., 2Grad. Sch. Argric. Sci., Tohoku, Univ., 3 Univ. of Gothenburg, Dept. of Chemistry andMolecular Biology)P-2н̧gǛвŏϟHѧӑҎֱ͓՟eØE|ɶ‫׃‬1ÜϨӯ΂, 1 ƚϨ Ԕ, 2 ‫מ‬ϱəƗ ‫װ‬1 ՎɈǱÏՎ‫ײ‬2 ȞȹЍՎ̱ʹϦѼƙʮӨÌÐ͉̱Згʩ‫ױ‬ǛвŏϟSZн̧‫ײ‬°Æӑɞ̀ǖgǖǎǗ̧bS`ϧC|Oba‫ײ‬ȆȔĒgƎ։HǣŧU|ObĤzGed]`C|9SGS‫ײ‬ǛвŏϟHȆȔĒgƎ։ǣŧQW|ƿ̲֛Ġe^C`h×̂dπHǮC9Әҗzh‫ײ‬н̧gǛвŏϟ̠֚Üeѧӑ‫׌‬Hн̧eēzGgԺЃǫŴvZzSZga
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Effects of aging treatment on bacterial community in bamboo sawdust
Yuji Nakada, 1Makoto Yoshida, 2Koji Takabatake

1(1Tokyo Univ. of Agric. and Technol., 2Toyama Prefectural Agric, Forestry and Fisheries Research Center, Forestry
Research Institute)- 39 —P-3
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Chiaki Hori (CSRS, RIKEN), Kiyohiko Igarashi, Masahiro Samejima (Agri., Univ. of Tokyo), David Hibbett (Clark
Univ), Bernard Henrissat (CNRS), Jill Gaskel and Dan Cullen (USDA-FPL)

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Silai Zhang, Akihiko Ban, Naoki Ebara, Osamu Mizutani1), Mizuki Tanaka,TakahiroShintani, KatsuyaGomi(Grad.Sch,Agric.Sci.,Tohoku Univ., NRIB1))- 40 —P-5͐ʂ։Ԋ˳ϧCZ˱ŴԫeβǌU|јϒӑgÀ©ÇÌƩϨ̛Ȇ 1‫ײ‬ïŹɃǍ˻Ȇ 2‫ײ‬ӟƆӯȆ 3‫ײ‬ӟðҏà 3‫ײ‬ȻϨ ĭ 1 ‫װ‬1 ̭õ˱ŴԫЗгʩÏģĥ‫ײ‬2 ̭Ǳ֤ÏՎϥЧ‫ײ‬3 õǱ֤ÏՎЧ‫ױ‬˱Ŵԫh‫̧ײ‬ԺHς͜Ϗ‫װ‬ΰƶ‫´ײ‬ÊÌdc‫ױ‬w̚͜Ϗ‫װ‬ѡ‫ײ‬Ѵ‫ײ‬Єׁ‫̧̡ײ‬dc‫ױ‬b͔:aB{‫̒ײ‬ֻQ}|ϣǢvΦɢΩɢdcgŘɿ×ҦdȵǭGzʐΦʐΩaB|ƀϏ‫ז‬bֿɓeǮ͔aB|9јϒӑ‫׌‬hO}zg˱ŴԫŧȘU|ƆƿӑgĀӪaB|H‫ײ‬O}zŧȘӑgЮ‫׌‬ḩԺwϣǢey]`Ǯ͔aB{‫ײ‬ſÒg˷·a֥֟U|Obhpq×ƔҦaB|9OgObGz‫ײ‬ħ:g˱ŴԫģԺŧȘU|ӑgŧȘ¿©¾eАЉSZȣŏ·ϧC|Oba‫˱ײ‬ŴԫĴuVeӑ֥ƈS‫ײ‬ŊϿϥʰŘU|ʮӨ
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Tomoko Wada1, Kiyohiko Igarashi2, Yuko Fujiwara3, Yoshihisa Fujii3, Ken Okada1(1Cent. for Conservation and Restoration Techniques, Nat. Res. Ins. for Cultural Properties, Tokyo, 2Grad. School of
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Takanari Togashi4, Seiichi Takami5, Masafumi Ajiri5, Manabu Fukumoto3, Keietsu Abe1, 6(1Grad Sch Agric Sci Tohoku Univ, 2Grad Sch Med Tohoku Univ, 3IDAC Tohoku Univ, 4Sci Yamagata
Univ.5IMRAM Tohoku Univ, 6NICHe Tohoku Univ)- 41 —P-7°ÆӑјeּҿĽřηØE`ʲϾΎʏϏԺϥϦU|ÔΒйɗ‫ײ׶‬ʦϨÒɭ 1‫ײ‬ӟðƀ 1‫ײ‬ȫɄ˦Ĥ‫ײ׶‬ÔƆҜ‫ײ׷‬ɏ̨́њ‫׶װ ׷‬ĤɆǱ֤Վ‫ ׷ײ‬Üֈɏ‫ױ‬ ϿĽÐ§‫ױװ‬Hˀ^ĽΫϐʏŗϧS`8°Æ‫ױ   װ‬ӑјgϥ֗eƊ

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Ryuhei Miura1, Kazuya Toda1, Hiroshi Fujii1, Masanobu Kojima1, Satoshi Mihara2, Masaki Ichimura2(1Grad. Sch. Agri., Shinshu Univ.; 2JA Nakanoshi)

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Mayumi Yamamoto1, Kosuke Izumitsu2, Yuki Kitade1, Kanako Hatoh1, Chihiro Tanaka1(1Grad. Sch. of Agri., Kyoto Univ., 2Sch. of Environmental Science, Univ. of Shiga Pref. )- 42 —P-9‫ש‬ӑ Aspergillus oryzae Ð¦³Ð͞˗̼eFM|ϴЮÌ¯ԺϥϦʏgƝÕȢ̤ Ƞ, Ƙű Οҍ, ȫȿ Ζɗ, ȌϨɅ ӯ̉, ‫ ̡ן‬ʈ, Ýȹ γÒ 1, ŵ̤ ŲjO 1‫¬װ‬º

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Improved production of a heterologous protein in Aspergillus oryzae by autophagy deficiency.
Hiroshi Teramoto, Kiyomi Kagami, Kaihei Kojima, Hiroaki Udagawa, Shinobu Takagi, Jun-ichi Maruyama1,
Katsuhiko Kitamoto 1(Novozymes Japan Ltd, 1 Department of Biotechnology, The University of Tokyo)

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L-lactic acid production from starch by simultaneous saccharification and fermentation in Aspergillus oryzae
Satoshi Wakai1, Toshihide Yoshie2, Nanami Asai1, Ryosuke Yamada1, Chiaki Ogino2, Hiroko Tsutsumi3, Yoji Hata3,
Akihiko Kondo2(1Org. Adv. Sci. Tech., Kobe Univ., 2Dept. Chem. Sci. Eng., Grad. Sch., Kobe Univ., 3Res. Inst., Gekkeikan)- 43 —P-11јϒӑ PKC g˶Ӹ֠ȘŞeȣU|ցͯÇÐ©Ìљg͓ьɝƗոǵ 1‫ײ‬ÜȹЎϩҍ 2‫ײ‬ƚӷƳ 2‫ײ‬ӟȻ̏Ś 3‫΀ײ‬ƙΟ 3‫ײ‬Ǚŉӯá 4, ͆ȹФ̂ 5, ֡պ˭ʕ 1,2 ‫װ‬1 ̭ŵ
Ǳ֤ՎÏϥϏϦ͏ţʣ, 2 ̭ŵǱÏ̢̫З‫ײ‬3 ½ŴȋɈ͏, 4 ̭Ǳ֤Վ, 6 ÜǵǱϟɈ‫ױ‬ѧҥǤ͓ь¨ÈĈի‫װ‬CWIS‫ױ‬ѫՂh‫ײ‬ЎӑgѧҥǤ͓ьeFC`ևӵdɷŢʹ]`C|9̤ѫՂ͓ʣU|Ì¯ԺgD\µÊ¤ Ì¨Ð C‫װ‬PKC‫ױ‬h‫ײ‬ϐeÜʆЃdɷŢʹ]`F{‫ײ‬јϒӑgÀ¥ÈϥϏ Aspergillus nidulans g PKC‫װ‬AnPkcA‫ױ‬w «Cv\Ϻӑg PKC‫װ‬MgPkc1‫ױ‬g͞˗HҹͦaB|ObHДz}`C|9OgObGzЎӑg PKC h˶ZdʲЎӑŞg͖ЃbS`ΉЉQ}`C|9ʤ:h‫ײ‬MgPkc1 g֠ȘŞˍѤЉЃeиĒ͓՟À¥ÇÌey| ÌÇÇÐ©Ìӧ]`
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Construction of yeast screening system for novel chemical agents that inhibit PKC of filamentous fungi.
Fumio Shoji1, Mayumi Nakayama2, Akira Yoshimi2, Tomonori Fujioka3, Kiyoshi Kawai3, Hiroyuki Horiuchi4,
Hideaki Umeyama5, Keietsu Abe1,2 (1Grad. Sch. Argric. Sci. & 2NICHe. Tohoku Univ., 3Kumiai Chemical Industry
Co., Ltd., 4 Grad. Sch. Agric. Sci. Tokyo Univ., 5Facul. Sci. Engin. Chuo Univ.)P-12‫ש‬ӑѼƙ¬¾¥Ð·Ðg֙ϿbŃ֙҉Ϩĭ 1‫ײ‬ÕϨΊǵ 3‫ײ‬ȼÖƩӯ 1,2‫װ‬1 տѼЗ‫ײ‬2 ɜȿǱÏļлЗ‫ײ‬3 ̼ɩćП¨Ç‫ױ‬‫ש‬ӑ‫װ‬Aspergillus oryzae‫ױ‬h8ȎŁʏH‫מ‬K8ΟտhTubU|ĈѱЃϿց‫׏‬ƪw‫׏‬ƪϧ8ŸϽϧgցѣgěѰΫ8Qzeh8ϴЮÌ¯ԺϥϦg¹¦bS`vĘϧQ}8ʤ:gϥΎe͞KObgŐ̫dCʂϥϏaB|9XgևӵʏGz8ɲʩah8‫ש‬ӑղĈȆӽ̰gÒũbU|Zue8‫ש‬ӑͱՈ¬¾¥Ð
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Ken Oda1, Yasuo Uemura3, , Kazuhiro Iwashita1,2 (1NRIB, 2Hiroshima Univ.,  3Genaris Inc.)- 44 —P-13
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Construction of cell surface display systems using hydrophobin (HypB) with functional peptides inAspergillus oryzae
Shintaro Ohashi, Keisuke Domae, Satoshi Nakama, Asuka Kase, Harushi Nakajima(Dept. of Agricultural Chemistry, Univ. of Meiji)P-14‫ש‬ӑ A. oryzae ϧCZ͋Ϗϩ̫À¡ÌgϥϦFykєӱ
ąӟǱĥ‫ײ‬ÝȹγÒ‫͵ײ‬ϨȐ͟ 1‫ײ‬ϨáĦĸ 1‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ײ‬1 ̭Ǳ֤ÏՎϥЧÏʊϥŴ‫ױ‬?ЉЃ@À¡Ìh‫͋ײ‬Ϗg֟ɿ¸µ¡§³½ÇÐeȷU|Ì¯ԺaB{‫ײ‬ցͯeȣS`ʲӑΎʏ̚U|ObHДz}`C|9rZ8ƭç‫׌‬ѧҥeFC`‫ײ‬ѕƏՑswҧҠփĀԠġբU|¥»«¡Ìb‫׌‬Čg͜Ҧv^ObHǝƤQ}`C| 1)9OgObGz‫ײ‬À¡ÌhѕȲϺwůҩЙŴdcgϥΎґʟϺˣƴU|ϏԺbS`̠ɺQ}`C|9‫ש‬ӑ Aspergillus oryzae hϴЮÌ¯ԺϥϦțÞbS`ŗϧQ}`F{‫ײ‬ǖǎÜeÌ¯ԺǱ։eőΆU|Ҧťv^9̤Згah‫׏ײ‬ϧaB|¦¼¦Fyk ¡ϩ̫gÀ¡ÌǱ։eɾ|ObЉЃbS`‫ײ‬A. oryzae ϧCZϥϦbєӱӧ]Z9?˷·ÏѬ̲@ʣψǒÀ¡ÌÐ§U| cDNA e^C`‫ײ‬A. oryzae a‫מ‬ϿϝS`C| tef1 ղĈȆg§ÌƠ̚ϙeƙW`ǡǗսœg̘կŴӧ]Z9Xg cDNA gÕΏe amyB ղĈȆӥƙS‫מײ‬ϿϝµÊÀÐÐP-glaA142 ŘɿÖaϿϝU|µÆ½§ĕӱSZ9À¡Ìeh HA Fyk His6 þŧS‫ײ‬AmyB bg֚e Kex2 ͔µÊ¤ÐŒ˴սœ˅ŀU|GZ\aԄԀSZ9ƘÀ¡ÌϿϝµÆ½§ϧC‫ײ‬A. oryzae µÊ¤Ð 10 ևИǦ̼ NSlD-ΔP10 2) țÞbS`ɳԺՆ˔ӧ]Z9Ə
ɾSZɳԺՆ˔̼ 5$DPY ǖǎa 4 ˻֚˄bDǖ‫ה‬SZbO~‫ײ‬ǖ‫ה‬ΙÜe¦¼¦Fyk ¡ϩ̫X}Y}gÀ¡ÌgϥϦЛԐSZ9Qze‫ײ‬Xgǖ‫ה‬Ι Ni-NTA ³©¤ÐÊ¼¦Æ³Ð
eěU|ObaÀ¡ÌєӱSZ9ϝǌ‫ײ‬ցͯ Saccharomyces cerevisiae aÀ¡ÌngʜƐʏe֛| Pho36p bЋƛʏv^ AO090003000602, AO090026000756 g͞˗̼ĕӱS‫ײ‬A. oryzae gʜƐʏĎÖQW|Obey]`À¡ÌgϥϦ։gǣŧԊs`C|91) Narasimhan et al. (2005) Mol. Cell 17, 171-180. 2) Yoon et al. (2011) Appl. Microbiol. Biotechnol. 89, 747-759.
Production and purification of plant osmotins in Aspergillus oryzae
Hiromasa ITO, Jun-ichi MARUYAMA, Koji NAGATA1, Masaru TANOKURA1, Katsuhiko KITAMOTO(Dept. of Biotechnol., The Univ. of Tokyo; 1Dept. of Appl. Biol. Chem., The Univ. of Tokyo)- 45 —P-15
јϒӑ Aspergillus nidulans α-1,3-ÈÌ͞˗̼gǖ‫ה‬ϐʏbϏԺϥϦʏgԉĝÒ̦́Ϙ1‫ײ‬ƚӷƳ2‫ײ‬Яӗ͇1‫֡ײ‬պ˭ʕ1,2‫װ‬1̭ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ײ‬2̭ŵǱÏ̢̫З‫ױ‬ʤ:h‫ײ‬јϒӑgÀ¥ÈϥϏaB|    eFC`‫ײ‬ѧҥǤ͓ʣǮѕaB|& ÈÌ‫װ‬AG‫ױ‬gƙʣցѣղĈȆg͜Ҧӽ̰բu`C|9O}Ւe‫̤ײ‬ӑeFM|ѧҥǤAGgϥƙʣhÞeղĈȆeĜȉU|Ob̂zGeSZ9rZΙĒ˄Јǖ‫̈ה‬ehֈϥ̼hӑјHŎֱS`ϞϒeϥҡU|geȣS‫̼˗͞ײ‬ИǦ̼ƊkíևИǦ̼hӑјHǖǎÜe
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Morphologic properties and penicillin production of Aspergillus nidulans α-1,3-glucan deficient mutants
under liquid culture conditions.
Masahiro Hitosugi1, Akira Yoshimi2, Azusa Inaba1, Keietsu Abe1,2(1Grad. Sch. Sci.,& Tohoku,Univ.
2NICHe, Tohoku Univ.)P-16΁їʂϥϏ Mortierella alpina 1S-4 g lig4 ղĈȆИǦ̼gĕӱӐɅȠƕ 1‫͝ײ‬Ԧӆ΂ 1‫ײ‬Ȏӟ̉Ӹ 1,2‫ײ‬ӖƙҍĐ 3‫ײ‬Οʹ ́ 4‫ײ‬ȫɅ ‫ ׅ‬1 ‫װ‬1 õǱ֤ՎÏʊϧϥƨ‫ײ‬2 õǱÏϥϟŴȋÃ©¢¦‫ײ‬3 Ì¦ÇÐÊÐ®È¬·ÐÄÌÌÐ̼ɩćП‫ײ‬4 õռȋǈǱÏ®

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ƝÕU|bëʙQ}| lig4 ղĈȆИǦ̼gĕӱԊsZ9?˷·@íƾóɋЋƛѪ˔EúU| lig4 ղĈȆИǦÌ¦Æ¦͓ьS8Ê®¤Ç¾·ey{ɳԺՆ˔ӧ]Z9ɾz}ZɳԺՆ˔̼g¬¾֐ǒbS8PCR ƊkÐÌey{ lig4 ղĈȆHѪ˔Eҋ˔Q}Z̼ձʳSZ9r
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A lig4 gene-disrupted strain from oleaginous fungus Mortierella alpina 1S-4
Hiroshi Kikukawa1, Eiji Sakuradani1, Akinori Ando1,2, Misa Ochiai3, Sakayu Shimizu4, Jun Ogawa1 (1Div. Appl.
Life Sci., Grad. Sch. Agric., Kyoto Univ., 2Res. Uni. Physiol. Chem., Kyoto Univ., 3Suntory Global Innovation
Center Ltd., 4Fac. Bioenviron. Sci., Kyoto Gakuen Univ.)- 46 —P-17
Aspergillus ȷӑgѧҥǤ&-1,3-ÈÌgÇѕŴbXg͜ҦʏԉĝƚӷƳ 18Яӗ͇ 28ГֈʣƩ 38Đ:̡Ȑ̂ 48ɼӟ̏ϥ 48ɅÕƩҏ 58֡պ˭ʕ 1, 2 (1 ̭ŵǱ̢̫З, 2 ̭ŵǱ֤Վ, 3 ȹɳǱ֤ϟɈ 4ÑȺ֝Æ£ӱ՟ʩ, 5 ̭ŵǱ֤Ÿ)
ʤ:h8Aspergillus ȷӑ‫װ‬A. oryzae8A. nidulans ‫ױ‬eFC`8ѧҥǤǮѕgÒ^aB|&-1,3-ÈÌ‫װ‬AG‫ױ‬e^C`Згբu`IZ9A. nidulans g AG ƙʣցѣղĈȆИǦ̼‫װ‬AG ͞˗̼‫ױ‬h8ѧҥǤ̸ҿʏg Congo Red w'-È¨Ðb¡¨ÐΎʏ̚U| Lysing Enzymes eȣS`‫מ‬CʜƐʏО
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U|ObĤzGed{8ЎӑgϥȉʥϲeFCM| AG gևӵʏHԐԢQ}^^B|9
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h8OgľϷѧҥag AG Çѕg͜ҦʏԉĝѬ̲e^C`vԣԜSZC9
Production of alpha-1,3-glucan oligosacchalides from cell wall of Aspergilli and its functional analysis
Akira Yoshimi1, Azusa Inaba2, Shigekazu Yano3, Hiroaki Sasaki4, Tomoo Goto4, Kazuyoshi Kawakami5, Keietsu
Abe1, 2 (1NICHe, 2Grad. Sch. Agric. Sci., 5Sch. of Med. Tohoku Univ., 3Grad. Sch. Sci. & Eng. Yamagata Univ.,
Gifu Shellac MFG)

4

P-18
‫ש‬ӑaϴЮϿϝQWZ³¥ÇÌϥƙʣցѣgѧҥŉȳǌӽ̰Ċ̓ɴ‫ײ‬ϨÜϡɌ‫ײ‬ӟȴϢɱ#⁾‫ײ‬žэʉ#⁾‫ײ‬ƊɅӆЦ#⁾‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå‫̭װ‬ŵǱ֤ÏՎÏϥϏϦ͏ţʣ‫ײ‬ŵǱ֤Ïϟ‫ױ‬Քɘ‫ײ‬јϒӑw˥ѿӑgϥϦU|ǮЮgí͟ĀԠϦϏϥƙʣe֛|ղĈȆÆÐH͟:eƛȒQ}‫ײ‬ϥƙʣљ͓ʣU|ցѣҎϴЮțÞeȪŀS`‫מ‬ϥϦQWyDbCDԊsHЅed]`C|9Ȕ֬e‫͋ײ‬ϏϺƆӑ Phoma betae HϥϦU| DNA polymerase ֠ȘŞaB|³¥ÇÌ‫ש‬ӑAspergillus oryzae ey]`ϴЮϥϦQW|ObeʣŦS`C|#⁾9XOa̤Згah‫ײ‬ϥϦ։gƝÕЉ
ˁS`ϥƙʣƍʊHӧ}|È«ÆwˋŐe֛|¦ÆÌ»ÐÐ‫װ‬TP‫ױ‬eАЉS‫ײ‬X}zgѧҥŉȳǌӽ̰ӧ]Z9$ƘϥƙʣցѣwÈ«Æ¼ÐÐÌ¯Ժ GFP rZh RFP bgӥƙÌ¯ԺbS`ŅϿϝQW‫ײ‬ӤĽ‫׋‬ʂ֕ӻȡӧDOba‫ײ‬ӥƙÌ¯ԺgȳǌպčƛȒSZ9XgѬ̲‫ײ‬³¥ÇÌϥƙʣƍʊg̘ѩ֪ͫe֛| 2 Ю‫׌‬g Cytochrome P450 hȫҥĒngȳǌHԐuz}ZH‫ײ‬ӾȄ§¿ÌHȫҥĒŉҭeB|gGcDGƠu`‫̕ײ‬d|ӽ̰բu`C|9rZ‫̘ײ‬ѩϦϏgˋŐe֛]`C|bˏȒQ}| TP hѧҥŉaҺƦΛC˂ůОS‫ײ‬ŬϙЃdˋŐbCDӻπGzvևӵʏHОƮQ}
ZZu‫ײ‬Ȕ֬e³¥ÇÌϥϦeØE|ɶ‫׃‬e^C`͌ԁvӧ]`C|91) Fujii et al., Biosci. Biotechnol. Biochem., 75, 1813-1817 (2011)
Subcellular localization of aphidicolin biosynthetic enzymes expressed in the heterologous host, Aspergillus
oryzae.
Akihiko Ban, Mizuki Tanaka, Ryuya Fujii¹⁾, Atsushi Minami¹⁾, Hideaki Oikawa¹⁾, Takahiro Shintani, Katsuya Gomi
( Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ., ¹Hokkaido Univ.)- 47 —P-19
Involvement of lectin-type cargo receptors in heterologous protein secretion in Aspergillus
oryzaeDung Huy Hoang, Jun-ichi Maruyama, Katsuhiko Kitamoto (Dept. of Biotechnol., The Univ. of Tokyo)
Aspergillus oryzae has a superior capacity to secrete a large amount of proteins into the medium, and is being
industrially used for the production of commercially important proteins. In order to further improve the strain,detailed knowledge about its secretory pathway is crucial. However, current understanding on how elements of A.
oryzae’s secretory pathway interact with secreted proteins is still very scarce. Cargo receptor is a class of membraneproteins that are responsible for the efficient recruiting and packaging of cargo proteins into anterograde or
retrograde vesicles for trans-organelle transportation. One subclass of cargo receptor, the lectin cargo receptor,
specifically interacts with glycoproteins via their lectin domain. In recent years, lines of evidence are implying that
lectin cargo receptor not only recruits cargo proteins into transport vesicles but also functions in quality control ofsecretory proteins. It is then very interesting to investigate the relationship between lectin cargo receptors andheterologous proteins in A. oryzae. The mammalian Vip36 and yeast Emp47p are well-studied lectin cargo receptors,
with the former being reported to be involved in post-ER quality control of certain secretory proteins. A BLAST
search on the A. oryzae genome database returned two homologs of these two proteins. The two genes ID are
AO090026000428 and AO090102000145, which were named Aovip36 and Aoemp47, respectively. Fluorescencemicroscopy observation revealed that the two lectin cargo receptors mostly colocalized with an early Golgi marker.
Deletion of each cargo receptor gene improved the secretion of heterologously expressed bovine chymosin andhuman lysozyme into the culture supernatant. Moreover, analysis of the ER-enriched fractions revealed that deletionof each gene reduced the degree of heterologous protein accumulation, suggesting that those two cargo receptors
may be involved in the retention of heterologous proteins along the secretory pathway. Further studies to investigate
the interaction mechanism between those two cargo receptors and heterologous proteins are being conducted.

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 rV‫ש‬ӑgɲցѣղĈȆgˍѤӧ]Z9ցͯ S. cerevisiae w A. nidulans сýgЎӑaƛȒQ}`C|ɲցѣeȣS`‫ש‬ӑ¬¾gŁғԆϦϏЋƛʏ͌ѤSZѬ̲‫ײ‬ɲցѣeϐʄЃd§¿ Ìgսœ̂Лev^ղĈȆH 6 ħЛԐQ}Z9͟eO}zgƘИǦ̼ĕӱS‫ײ‬ӪϝǒÏҧҠփԵŴҦÏӑĒŉҧҠփ։сԙoZ9XgѬ̲‫ײ‬ɲ¹ÀÊg 1 ħh͞Ƕey{ӑĒŉҧҠփ։ƝÕQW‫ײ‬Ŗg 1 ħhҧҠփԵŴeÞeĶKObĤzGbd]Z9ýg 4 ħh͞ǶS`vӪϝǒсHǫŴSdG]ZZu‫ײ‬ϥҡeF
M|ɷŢhŕzdG]Z9ùɼ‫ײ‬ӑĒŉҧҠփ։HƝÕSZ̼aýgղĈȆѪ˔E`‫̕ײ‬d|ƝÕЉˁUëȒaB|9
Analysis on the Roles of Six Fatty Acyl-CoA Synthetase Homologs in the Growth of Aspergillus oryzae
Koichi Tamano1, Kenneth Bruno2, Hideaki Koike1, Tomoko Ishii1, Ai Miura1, Myco Umemura1, Scott Baker2,
Masayuki Machida1 (1AIST, 2PNNL)- 48 —P-21‫ש‬ӑ A. oryzae eFM|¦ÉʊуԙъÌ¯Ժ AoRim15 g͜Ҧӽ̰Ų̈ӆΜ‫ײ‬Ӑ֚֩ʉ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬?ЉЃ@ցͯg Rim15 h¦ÉʊуʏՆŋƿȆgÕΏeB{‫ואה̻ײ‬ed|bΎʏŴS‫ײ‬ҥȆɳʣԒȪU|bҖEz}`C|9rZ‫ײ‬Οտցͯh‫ײ‬Ȕ‫כ‬ȕցͯbͱՈS`‫מ‬CÈÐÈϿցť̚S`
F{‫ײ‬XgϟϩbS` Rim15 ղĈȆeFM|͜Ҧ͞ǶǫϴHǝƤQ}`C|9̤Згah‫שײ‬ӑ Aspergillus
oryzae g Aorim15 g͜Ҧӽ̰ЉЃbSZ9
?˷·ÏѬ̲@Rim15 eЋƛdÌ¯ԺÐ§U|‫ש‬ӑ A. oryzae Aorim15 e^C`‫ײ‬ИǦ̼Əɾ
SZ9Ӫϝǒӽ̰ӧ]ZѬ̲‫ײ‬PD ǖǎahֈϥ̼bͱՈS`őϥȆH‫׋‬ӘeΤȬSZ9Ò˷‫ײ‬È±¦ÐÈŀ{‫מ‬Η՛ǋǖǎah‫ײ‬ֈϥ̼bgőϥȆˮgɋHΤȬSZ9rZ‫ײ‬ИǦ̼ah‫ײ‬ֈϥ̼bͱՈS`ωeʜƐʏОUObHӻȡQ}Z9Qze‫ײ‬ֈϥ̼b Aorim15 ИǦ̼e‫ײ‬EGFP,AoRim15 aÚ̼gȳǌԙoZbO~‫ײ‬ѧҥԺeӤĽHӻȡQ}Z9āÕgѬ̲y{‫ײ‬S. cerevisiae bƛ͔e A. oryzae av‫ײ‬
AoRim15 HΗ՛ǋwω¦É‫ײ‬őϥȆɳʣe֛ØS`C|ObHОƮQ}Z9Ò˷a‫ײ‬Οտցͯg Rim15h³ÉÐ¾³¦ǫϴHϥTZObey{‫ײ‬C ̣ĮH͞˗S`C|9XOa‫ײ‬Οտցͯg Rim15 bƛ͔e
AoRim15 gˏȒQ}|¦Éʊуԙъ§¿ Ìg C ̣лĮÒպ͞˗QWZ̼ĕӱSZ9ϝǌ‫ײ‬Og̼ϧC`‫ײ‬ֈϥ̼bИǦ̼gӪϝǒӽ̰ÜaB|9rZ‫ײ‬ИǦ̼bƛ͔eωdcg¦ÉʜƐʏԊ‫כ‬vӧDëȒaB|9Functional analysis of the stress response regulator protein AoRim15 in Aspergillus oryzae
Hidetoshi NAKAMURA, Takashi KIKUMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo)

P-22
јϒӑ Aspergillus nidulans ey|Ǌǧ³½ÌփgĀԠ͓͜ÜεǸҍ‫ײ‬ҕ΃ЗÒ‫מײ‬ԦЊ͚ ‫װ‬тΈǱ֤ÏϥƨϣǢ‫ױ‬ǊǧeԧȞeƠr}|³½Ìփh, ͋ϏÇ©Ìϩ̫gӂ‫ח‬ϣwҧҠ˹֔ǮKƠtֶőӽʏg‫מ‬őȆŴƙϏaB{‫ײ‬ҷτϮahϥϏÏֿϥϏЃeőӽQ}ǎϞÕgοѣʁϣHѸˀQ}|9SGSdHz‫ײ‬ÒպgѧӑwЁҿҬ̥ӑ֥C`‫ײ‬³½ÌփgϥϏőӽe^C`gpbch̢ӽ̂aB|9Ò˷‫̤ײ‬Згȕah‫ײ‬O}rae‫ײ‬188 ЮgјϒӑgD\ 15 ЮHǖǎÜg³½ÌփҪҿU|ΎʏОUObbO}zgǮKHǖ‫ה‬eĊC³½ÌփĎőȆŴU|ObӷŐS`IZ‫̆װ‬ɘɢ8̤Ì³ÉÌϿӪ P-8‫ױ‬9̤Згah‫ײ‬³½ÌփgĎőȆŴҦHɰG]Z Aspergillus nidulans ϧC`‫ײ‬јϒӑey|³½ÌփĀԠgőȆ͓͜ӽ̂U|ObԊsZ9̘ȬΙĒǖǎe A. nidulans gőϥȆΞŧS` 12 ֚̈˄bDǖ‫ה‬SZɼ8O}e 0.1‫ׯ‬³½ÌփΞŧS‫ײ‬Qze 3؈24 ֚̈ǖ‫ה‬SZӑĒGzŁ RNA ʸŐS‫ײ‬DNA¼ ÊÉ ӽ̰ӧ]Z9ղĈȆϿϝ¯ÐÌey|ÆÐӽ̰gѬ̲‫ײ‬³½ÌփgΞŧeĊCϿϝHÕ̀U|ÆÐeh‫ײ‬ÈºÌփ‫̚͜ײ‬Ժ‫ײ‬ҧҠփ‫װ‬GO No. 46395‫ײ‬1901575‫ײ‬6631‫ױ‬gĀԠղĈȆHǮKӷz}Z9rZ8ӂ‫˹ח‬gőӽe֛| Flavin-containing monooxygenase Fyk Cytochrome
P450 ղĈȆҎgD\8X}Y} AN105828AN7522 gϿϝHÕ̀S`F{8ϝǌO}zg͜Ҧe^C`gӽ̰UUu`C|9rZ‫ײ‬³ÐÇǫ˔ǒԻǭőĽӽ̰ey{‫ײ‬ǖ‫ה‬ɼgǖ‫ה‬ΙÜg³½Ìփahӂ‫ח‬ϣwÈºÌփgƠ։HĎÖU|ObHОQ}Z9āÕgѬ̲Gz‫ײ‬ǊǧϣǢÜev͊ʑU| A. nidulans
H³½ÌփőӽU|Ob‫ײ‬јϒӑHǊǧ³½ÌփeȣS`ղĈȆϿϝÉ·ÈaʊуÏĀԠU|ObHŔu`ОQ}Z9

Analysis of soil humic acid degradation by the fungus Aspergillus nidulans
Nami Nakazawa, Ken-Ichi Oinuma, Naoki Takaya(Grad. School of Life and Environmental Sciences, Univ. of Tsukuba)- 49 —P-23‫ש‬ӑgӑјӥƙҦťgӽ̰˷·gЛиbǖǎʣőHØE|ɶ‫׃‬g͌ԁǠɁƩĖȆ‫ײ‬ÝȹγÒ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬?ЉЃ@‫ש‬ӑ Aspergillus oryzae h˻̤gĈѱЃdօ՟Ϧ͏eĘϧQ}`IZpG‫ײ‬ցѣϥϦwϴЮԺϥϦgțÞbS`vŗϧQ}`C|9A. oryzae HӑјӥƙӧDҦťv^Obh‫ײ‬FyX 50 ɘŜeǏƒԡÒչ̭õǱȋƜԏ˪ˊzey{ǝƤQ}`C|(Ishitani et al. 1956)9SGS‫ײ‬A. oryzae gӑјӥƙe֛S`‫ײ‬ЗгH‫ײ‬A. oryzae gҡЮaŗϧQ}|ObhdG]Z9rZ‫ײ‬ӑјӥƙh̚ʏϥͪagևӵd¤¢µgÒ^aB{‫ײ‬O}ӽ̰U|Obey{‫ײ‬r[ӷ^G]`Cd
C A. oryzae g̚ʏϥͪgϿӷe^dH|ƔҦʏHB|9ùƾʤ:h‫ײ‬A. oryzae gӑјӥƙҦťgӽ̰˷·ЛиS‫ײ‬ǖǎʣőey|ӑјӥƙҦťngɶ‫͌׃‬ԁSZ9?˷·bѬ̲@rV‫ײ‬A. oryzae eFC`‫ײ‬ÇÌÏÆÈӵͶʏ(ΔpyrG)b¥©ÌӵͶʏ(ΔadeB)g2 Ю‫׌‬g̼ĕӱS‫ײ‬ÚҗgőϥȆΝƙS`‫ײ‬ÇÌÏÆÈ‫ײ‬¥©ÌƠtȟǲǖǎe͋ӑS
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Investigation of nutrition effects in hyphal fusion efficiency of Aspergillus oryzae
Wakako TSUKASAKI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., The Univ. of Tokyo)

P-24
Aspergillus oryzae eFM| 331-25 sense RNA, 331-25 antisense RNA g͜Ҧӽ̰Րðְ‫͉ײ‬ϨȠ÷‫ײ‬ŜϨΓ‫ײ‬ȹɳΌɗ‫ײ‬нŉժ֯ ‫̭װ‬õՎɈǱÏʊϥЧ‫ױ‬?Ҥ̍bЉЃ@‫ש׫‬ӑgőϥȆƊkϿӄőϥȆg EST ӽ̰gѬ̲, 331-25 ղĈȆ‫ׇ‬ǕGzԗsŐQ}|mRNA hőϥȆegsӷŐQ}, 528 g EST gD\ 43 ƾbCD‫׉מ‬ɢaԗr}Z9rZ,őϥȆeFC` 331-25 sense RNA (āɼ SN RNA) b 331-25 antisense RNA (āɼ AS RNA) gÚ˷HԗsŐQ}`C|ObǝƤSZ9(1) ̤Згh 331-25 ղĈȆ‫ׇ‬ǕGzԗsŐQ},őϥȆϐϴЃeȉǌU|ObHˏΧQ}Z mRNA g͜Ҧ̂zGeU|ObЉЃbSZ9?˷·ƊkѬ̲@‫ש׫‬ӑg %brlA ̼ǖ‫ה‬SZőϥȆАϥЊŜgӑĒeh,ŻȒ։ PCR ey{ӑјehӷŐQ}dG]Z AS RNA HȉǌS`CZ9OgObGz, AS RNA hőϥȆ̷gɳʣbbveՆŋH֙ǿQ}|ObHОƮQ}Z9͟e, 331-25 ղĈȆ‫ׇ‬Ǖ͞˗̼‫װ‬%331-25 ̼‫ױ‬ĕӱS,Ӫϝɳgӻȡӧ]Z9ѫ̈ЃeőϥȆˮ,Ê©ÐЊɹgԀΧӧ]ZbO~, %331-25 ̼hÌ¦ÊÐÈ̼bͱՈS`,őϥȆˮgĎÖ,Ê©ÐɹHȫQKd]`CZ9rZ, 331-25 ղĈȆ‫ׇ‬Ǖg͞˗ey|Ͽӄϙngɶ‫׃‬hӷz}dG]Z9Qze,ĽφȦ̪ă(̂ʩ,ǼͳЃ̪ă)aӪϝɳgӻȡӧ]ZbO~,őϥȆ̷gɳʣeɶ‫׃‬Hӷz},ĽֿφȦ̪ă‫̑װ‬ʩ,ȅͳЃ̪ă‫ױ‬aӪϝɳgӻȡӧ]ZbO~, 331-25 ղĈȆ‫ׇ‬Ǖg͞˗ey]`őϥȆˮHÕ̀S`CZ9āÕgOby{, 331-25 ղĈȆ‫ׇ‬ǕGzԗsŐQ}Z SN RNA,AS RNA hĽφȦ̪ă,ĽֿφȦ̪ăgÚ˷eFC`őϥȆɳʣҦeɶ‫׃‬S`C|ƔҦʏHОƮQ}Z9(1)Րðz, п 12 ƾјϒӑőȆϥϏȋÌ³ÉÌӵ˼ֱ p. 46 (2012)
Analysis of 331-25 sense RNA, 331-25 antisense RNA in Aspergillus oryzae
Masaru Tsujii, Hiroto Morita, Hiroshi maeda, Yohei Yamagata, Michio Takeuchi
Dept. of Applied Biological Science, Tokyo Univ. of Agriculture and Technology- 50 —P-25‫ש‬ӑ A. oryzae eFM|ӑ̽ɳʣʰŘƿȆ EcdR ͞˗ey|̚ʏϥͪϿӷgԊsϨÜ ŭͳ‫ײ̬֎ ֊ײ‬Ýȹ γÒ‫ײ‬ŵ̤ ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬?ЉЃ@‫ש‬ӑ Aspergillus oryzae eFC`̚ʏÙĀhϿӷQ}`CdCH‫̼ײ‬ey]`ϴd|ˎƙǒղĈȆ
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SZ̼ĕӱSZ9ņĒЃeh‫ײ‬A. oryzae gX}Y}gˎƙǒ̼eFC`‫ײ‬ÇÌÏÆÈӵͶʏrZh¥©ÌӵͶʏþŧU|bbve‫ײ‬EGFP vSKh mDsRed gϿϝey|ӤĽa͖ԢSZ9Qze‫ײ‬
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Qze‫ײ‬ӑ̽ʣψQWŉպ͓՟ӻȡSZbO~‫̚ײ‬ʏҥȆb‫׌‬ČSZɳʝЃϐʄv^͓՟HӻȡQ}Z9ϝǌ‫ײ‬ǖ‫̪ה‬ăg͌ԁw̚ʏϥ֛ͪՠղĈȆgթšϿϝey]`‫ײ‬A. oryzae g̚ʏϥͪϿӷU|ObԊs`C|9
Promotion effects of deleting a sclerotial formation repressor EcdR for discovery of sexual reproduction in
Aspergillus oryzae
Yuki TANAKA, Feng Jie JIN, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., The Univ. of Tokyo)

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Localization and functional analysis of the autophagy-related membrane protein AoAtg9 in Aspergillus
oryzaeKohei Fujiki, Takashi Kikuma, Katsuhiko Kitamoto(Dept. of Biotechnol., Univ. of Tokyo)- 51 —P-27   gѧҥǤ¦ÉҘʏe֛|͜Ҧ̢ДղĈȆgӽ̰ʃ͵Ǹǵ ‫ײ‬ǾȱƕȆ  ‫ײ‬íСΊǗ ‫ײ‬нɅӝ ‫ײ‬ȼÖƩӯ  ‫ײ‬ɼӟ͢ŗ  ‫ װ‬äǱ֤ÏϥԵϣ‫ ײ‬äǱ֤ÏՎ‫ ײ‬ɜǱ֤؊ļлЗ‫ ײ‬տѼЗ‫ױ‬?ЉЃ@¬¾ӽ̰gѬ̲‫שײ‬ӑgv^ղĈȆg 50%āÕh͜Ҧ̢ДaB{‫ײ‬XgǮKhјϒӑaģȉQ}`C|ObĤzGed]`C|9O}zgղĈȆgӽ̰h‫ײ‬јϒӑϔҷgϥƨϝԨgӽ̂e^dH|vgbS`̠ɺQ}|9̤Згah‫ש׫ײ‬ӑ Aspergillus oryzae gv^јϒӑeϐϴЃd͜Ҧ̢ДղĈȆgD\‫ײ‬XgղĈȆИǦ̼HѧҥǤƙʣ֠ȘŞeȣS`‫מ‬ʜƐʏОU AO080569000108 g͜Ҧӽ̰ӧ]Z9?˷·bѬ̲@AO080569000108 ИǦ̼h‫ײ‬ֈϥ̼bͱՈS`‫ײ‬ӑјċ֗ҦFykőϥȆɳʣҦHĎÖ
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Functional analysis of functionally-unknown gene related cell wall stress resistance in Aspergillus oryzae.
Nao Tokunaga1, Ayako Senoo3,4, Taiki Futagami2, Kaoru Takegawa2, Kazuhiro Iwashita3,4, Masatoshi Goto2(1Grad. Sch. Biores. Bioenviron., Kyushu Univ., 2Faculty of Agriculture, Kyushu Univ.,
3

Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 4NRIB)

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ցͯg Gap1 a͜ҦgլDπHsz}Z9ϝǌ‫ײ‬ǖ‫̈ה‬g½¬փȉǌÖagϥҡwƏ{Ցse^C`‫ײ‬
Qzd|Ԏѧdӽ̰ӧ]`C|91)Đֈz‫̤˻׾‬ՎӃŴȋć 2012 ɘɢǱć
Functional analysis of the Aspergillus oryzae amino acid transporter
Hiromu Hamanaka, Akihiro Kaneko, Harue Kitagawa, Motoaki Sano, Kenji Ozeki, Shinichi Ohashi (KIT)- 52 —P-29
Aspergillus nidulans eFM|µÊ¤
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Functional analysis of Protein kinase C in sterigmatocystin biosynthesis in Aspergillus nidulans
Takuya Katayama and Hiroyuki Horiuchi (Dept. of Biotechnol., Univ. of Tokyo)


P-30
Aspergillus nidulans eFM|őϥȆϐϴЃĎőȆÌ¯Ժ CON ¹ÀÊgӽ̰֋̡Ҝ a‫ײ‬b‫ ײ‬Özlem Sarikaya Bayrama‫ ײ‬Özgür Bayrama‫ ײ‬Gerhard H. Brausa‫װ‬a ¢¤ÌÌǱȋ‫ ײ‬b ‫׏‬ѼЗ‫ױ‬
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Satoshi Suzukia‫ײ‬b‫ ײײ‬Özlem Sarikaya Bayrama‫ ײ‬Özgür Bayrama‫ ײ‬Gerhard H. Brausa(a Georg-August-Universität Göttingen‫ ײ‬b NFRI)- 53 —P-31
Aspergillus nidulans gÇÐÌĀԠb¤Ç¼¦¡Ìϥƙʣȫ̮Ɂʛֆ‫ײ‬Ǫ̃̔ú, ‫מ‬ԦЊ͚ ‫װ‬тΈǱ֤ÏϥƨϣǢ‫ױ‬Aspergillus nidulans g AmyR h8½ÆÐղĈȆgՆŋΎʏŴQW|ՆŋƿȆbS`Дz}`C|9Ò˷8ʤ:h AmyR H8̤ӑHϥϦU|í͟ĀԠϦϏaB|¤Ç¼¦¡Ì‫װ‬ST‫ױ‬gϥʣʰŘS`C|ObӷŐSZ9rZ8AmyR ղĈȆgղĈȆИǦ̼‫װ‬ΔamyR‫ױ‬hֈϥ̼bͱՈS`Ǯ։gÇÐÌѧҥŉeӚвS`CZ9̤Згah8AmyR ey| A. nidulans gí͟ĀԠgϿϝʰŘ¿©¾gӽ̂ЉˁS8̤ӑgÇÐÌĀԠeАЉSZ͌ԁӧ]Z9ΔamyR bֈϥ̼ÈÐοѣΫbSZ̘ȬȟǲǖǎϧC`ǖ‫ה‬SZ֬gղĈȆϿϝgѫ̈ǫŴ DNA ¼ ÊÉ ӽ̰ϧC`ӽ̰SZ9XgѬ̲8ΔamyR ah8ST [MadKýg PKSÏNRPS Ơtí͟ĀԠљgϥƙʣղĈȆÆÐgղĈȆgϿϝHҪʰŘQ}`C|ObĤzGbd]Z9̤ǖ‫̪ה‬ăÖa AmyR ey]`ϿϝHԒȪQ}|½ÆÐ AmyB gղĈȆИǦ̼‫װ‬ΔamyB‫ױ‬h8
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Analysis for glycogen and sterigamtocystin metabolisms by Aspergillus nidulans
Airi Komatsuzaki, Yosuke Kamimura, Naoki Takaya(Grad Sch Life Environment Sci, Univ. of Tsukuba)

P-32‫ש‬ӑ¼È¦Ð¯Ð½ÐőӽeFM| HECT Ã±¡ÌÇÐ HulA g֛Ø̮ΒĸĖ‫ײ‬ϨÜϡɌ‫ײ‬ɗ̤Ƭå‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå ‫̭װ‬ŵǱ֤ÏՎÏϥϏϦ͏ţʣ‫ױ‬ѧҥhǭպϣǢeʊT`×ӵbd]ZҲÌ¯Ժ՞wGeőӽU|͓͜̚S`C|9ʤ:hO}
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Involvement of HECT ubiquitin ligase, HulA, in degradation of maltose permease in Aspergillus oryzae
Yuka Matsuura, Mizuki Tanaka, Tetsuya Hiramoto, Takahiro Shintani, Katsuya Gomi
(Div.Biosci.Biotechnol.Future Bioind.,Grad.Sch.Agric.Sci.,Tohoku Univ.)- 54 —P-33 (O-1)јϒӑgѧҥԺ×Ʃƙʏƍʊ̈eFM|µÊÆ¾ѧҥ͓ͦ͜gӽ̰1Õ͉ƵǱ‫ײ‬1 ðÕŧǸȆ‫ײ‬1 ̡Ϩź̎‫ײ‬1 Üȵ˰ǐ‫ײ‬2 ň̮ҜȆ‫ײ‬1 ͺϨĭÒ ‫װ‬1 СʦǱ֤ÏՎȋЗгЧ‫ײ‬2̲͚З‫ױ‬
̲͚‫׌‬ЁѝҐϺӑeȣSӑ‫ ׌‬ÈϧCZϥϏ֥֟·aB| Virocontrol ·h‫ײ‬ӑјӥƙúSZ
¼  ÈgĈ˘HևӵaB|9SGS‫ײ‬ղĈȆǒgϴd|ӑ̼֚ah‫ײ‬ѧҥԺ×ƩƙʏƍʊԽOS‫ײ‬ӑјӥƙU|ObdKѧҥͦɫIԽOUOby{‫ײ‬ӑ‫ ׌‬ÈgĈ˘Hǁֶbd|9̤ЗгahѧҥԺ×ƩƙʏƍʊeFM|ѧҥƍʊe^C`‫ײ‬ѧҥȋÏőȆϥϏȋЃӽ̰ӧ]Z9×ƩƙʏƍʊeF
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Cytological and transcriptional analyses of heterogenic incompatibility in Rosellinia nacatrix.
1

Takahiro Uwamori, 1Kanako Ikeda, 1Chiaki Kida, 1Hitoshi Nakayasiki, 2Satoko Kanematsu, 1Kenichi Ikeda(1Grad. Sch. Agric. Sci, Kobe Univ. 2Nat. Inst. Fruit Tree Sci. NARO)

P-34‫ש‬ӑ A. oryzae eFM| 2 ^g acyl-CoA binding protein (AoAcb1‫ײ‬AoAcb2)gӽ̰ɅƒҼɗ‫ײ‬Ӑ֚֩ʉ‫ײ‬ŵ̤ŲjO ‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬ՔɘǮKgЎ̽ϥϏeFC`‫ײ‬ȫҥĒbÈĒúSZ՝ɓgőΆѫՂbhϴd|͔:dѫՂagÌ¯ԺőΆHǝƤQ}`C|9XgÒ^bS`‫ײ‬ցͯeFC`‫ײ‬ϐͨőΆѫՂey| acyl-CoA binding
protein (Acb1)gѧҥǭngőΆHǝƤQ}`C|9Acb1 eh¨ÈսœHȉǌWV‫ײ‬Acb1 gϐͨőΆѫՂehǮKgÐ¦³Ð֛ՠÌ¯ԺHʇӵbQ}|9ցͯah ACBP (Acyl-CoA Binding
Protein)À¡Ð³̚U|Ì¯Ժh Acb1 [MaB|H‫ײ‬јϒӑgǮKah ACBP À¡Ð³ˀ^Ì¯ԺH 2 ^ȉǌU|9̤Згahјϒӑ Aspergillus oryzae eFM| 2 ^g acyl-CoA binding protein(AoAcb1‫ײ‬AoAcb2)gӽ̰ӧC‫ײ‬ϐͨőΆѫՂϿӷgʪHG{ɾ|ObЉЃbSZ9
AoAcb1-EGFP hѧҥŉa§¢¦ϒ͓՟b]`F{‫ײ‬ʂȫчĜȉЃeЫůU|͔ȆHӻȡQ}Z9Ò˷ AoAcb2-EGFP hѧҥԺeɜKȉǌS`CZ9Aoacb2 ИǦ̼ɾ|ObhaIdG]ZZu‫ײ‬thiA µÊÀÐÐa Aoacb2 ϿϝŘɿSZbO~‫ײ‬Ê©ÐŻɹgΤȬwőϥȆˮgĎÖHӻȡQ}Z9Ð¦³ÐԒȪe֛ØU| Aoatg1‫ײ‬Ð¦³Ð¾Ҳɳʣ‫ײ‬ċ֗e֛| Aoatg4 b Aoatg8 gИǦ̼ah AoAcb1 HѧҥԺaŎֱS`ЫůӧdKd]Z9Ò˷‫ײ‬Ð¦³¢º¥Ðőӽe֛ØU|
Aoatg15 gИǦ̼ah AoAcb1 gѧҥԺagŎֱhӷz}dG]ZH‫ײ‬ÒպHΙҥŉaӻȡQ}Z9āÕgѬ̲Gz‫ײ‬AoAcb1 gՍՙw͜ҦehÐ¦³Ð¾ɳʣe֛ՠU|Ì¯ԺH֛ØS`C|O
bHОƮQ}Z9ϝǌ‫ײ‬Aoacb2 Řɿ̼gԎѧdϥҡgͱՈ‫ײ‬AoAcb1 b AoAcb2 gѧҥǭngőΆe^C`ӽ̰բu`C|9
Analysis of two acyl-CoA binding proteins (AoAcb1, AoAcb2) in Aspergillus oryzae
Kouhei KAWAGUCHI, Takashi KIKUMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo)- 55 —P-35 (O-2)‫ש‬ӑ A. oryzae gӑј˗ĴɼgѧҥĥʀeFM| AoSO g͜Ҧӽ̰Ʌϯѯɗ‫ײ‬ĐĉǍ‫ײ‬ÝȹγÒ‫ײ‬ŵ̤ŲjO‫̭װ‬Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬?ЉЃ@‫ש‬ӑ Aspergillus oryzae HȷU|¡ÁËÌñ֘‫װ‬Ў͢Ȇƻӑѹ‫ױ‬gјϒӑeFC`‫ײ‬ӑјh֫Ǥey]`ǮKgѧҥeŷŒz}`C|9֮ˎU|ѧҥh֫ǤȇúS`ѧҥ֚ՠѮӧDH‫ײ‬B|ѧҥH˗ĴSZ֬eh֫ǤȇlQCa‫ˎ֮ײ‬U|ѧҥeέӑHĈ˞U|g֟L͜ҦĲE`C|9
SGSdHz‫ײ‬έӑ̈gѧҥĥʀbŊϥ֗eFM|őȆ͓͜h̢[ӽ̂Q}`CdCպőHǮC9ʤ:hāŜ‫ײ‬A. oryzae eFC`¯ÈÉÐÐϧC`‫ײ‬ѧҥeթǱdԪʹGMVeέӑQW‫ײ‬Ŋϥ֗raӻȡaI|˶ZdȔ‫·כ‬ЛиSZ9XS`‫ײ‬AoSO Ì¯ԺHέӑЊɼe֫ǤȇnŎֱS‫ײ‬Ŋϥ֗e֛ØU|Ob̂zGeSZ9̤Згah‫ײ‬AoSO ƊkO}bЋîĕϧU|bҖEz}|Ì¯Ժgӽ̰՝S`ѧҥĥʀ͓͜gőȆ͓͜gӽ̂ԊsZ9?˷·ÏѬ̲@AoSO ģȉQ}`C|‫ׇ‬ǕPbe 3 ^eŷŒ{‫ײ‬N ̣л‫ׇ‬Ǖ‫װ‬1-555 ϳg½¬փ‫ױ‬b C ̣л‫ׇ‬Ǖ‫װ‬1147-1195 ϳg½¬փ‫ױ‬X}Y}͞˗QWZպő͞˗ĒϿϝU|̼eFC`‫ײ‬έӑŜɼeFM| AoSO gȳǌǫŴӻȡSZ9XgѬ̲‫ײ‬C ̣л‫ׇ‬Ǖg͞˗gǞƙh֫ǤȇeŎֱU|
ӑјgŢƙHΤȬS‫ײ‬Ŋϥ֗͜ҦgĎÖbÒҹU|Ѭ̲Hɾz}Z9Ò˷‫ײ‬N ̣л‫ׇ‬Ǖg͞˗eFC`‫ײ‬֫ǤȇeŎֱU|ӑјgŢƙhǫŴSdG]ZH‫ײ‬ŎֱU|֚̈H AoSO Ł֗eͱo`֗Kd{‫ײ‬թš
eŎֱU|͔ȆHӷƐMz}Z9Qze‫ײ‬AoSO bЋîĕϧU|bҖEz}| 2 ^g˶ӸÌ¯Ժe^C`‫ײ‬X}Y}Ð§U|ղĈȆИǦ̼ƏɾSZ9¯ÈÉÐÐey{έӑQW|Ȕ‫כ‬ӧ]ZѬ̲‫ ײ‬AO090005001564 ղĈȆИǦ̼e֛S`Ŋϥ֗͜ҦgĎÖHӻȡQ}Z9rZ‫ײ‬Aoso Ɗk
AO090005001564 ղĈȆИǦ̼eFC`h‫¡ײ‬ÌևƙġբÌ¯ԺaB| AoBni1 Hέӑɼg֫ǤȇaթšeӚвU|͔ȆHӻȡQ}Z9ϝǌ‫ײ‬AoSO b AO090005001564 g͜ҦЃ֛Ġe^C`ӽ̰բu`C|9Investigation of AoSO function in cell repair after hyphal wounding in Aspergillus oryzae
Junpei KAWABATA, Kei SAEKI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., The Univ. of Tokyo)

P-36
Aspergillus oryzae g­§Ê³Ð±ÌҎg͜ҦFykȳǌʏgӽ̰ȹɅѬ‫ײ‬ЖϨźѲ‫˽ײ‬ɅӀđӓ‫ʹײ‬ֈđ‫ײח‬Üȿ̅ѥ ‫΂̂װ‬ǱÏՎÏՎŴ‫ױ‬­ §Ê³Ð±Ì‫װ‬hydrophobin‫ױ‬h‫ײ‬јϒӑÏʹȆӑ‫׌‬esz}|ѧҥӪȸÌ¯ԺaB|9ĎőȆ։‫װ‬70~150a.a.‫ױ‬gÚӺȄʏÌ¯ԺaB{‫ײ‬ӑĒǭeőΆQ}Zɼ‫ײ‬ӑјwőϥȆgӪȸeҷɌֱƙS`ſȸɳʣS‫ײ‬ӑĒe˝ʹʏþØU|9Òҽe‫^׶ײ‬gӑ̼hîCeЋƛʏgĎC­ §Ê³Ð±ÌղĈȆӲˮ̚S`C|H‫ײ‬XgĘCőMe^C`hpbcӽ̂Q}`CdC9O}ragЗгa8‫ש‬ӑ Aspergillus oryzae g­ §Ê³Ð±ÌղĈȆ hypA,B,C gϿϝЛԐS`C|9hypA,B,C e^C`ИǦ̼ĕӱS, HypA hÞbS`őϥȆe‫ײ‬HypB hӑјeȳǌS‫ײ‬HypC h՝ɓg̪ăÖahϿϝ։HȬdCObӻȡS`C|9Qze‫ײ‬hypA ղĈȆhőϥȆɳʣ̠e‫ײ‬hypB ղĈȆhӑјgċ̠֗eՆŋ։HǣŧU|ObӷŐS`C|9XOa‫ײ‬HypA b HypB gȳǌʏbϿϝg֛ՠ
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Characterization and localization of hydrophobins in Aspergillus oryzae
Yui Yamakawa, Chie Ishida, Fuyuka Hayakawa, Yuka Mizuno, Harushi Nakajima(Dept. of Agricultural Chemistry, Univ. of Meiji)- 56 —P-37‫ש‬ӑ Aspergillus oryzae g»ÇÇÌփĀԠb¦ÉʊуǮϨ Ŧϥ, Ǳƒ jG|, ͎̤ ʡÒ‫װ‬ՎЗ͓͜ ‫׏‬ѼЗ‫ױ‬ »ÇÇÌփh‫ײ‬ˮħGzˮЂħgÇÌփ‫װ‬È¦ÇÌփ‫ױ‬H‫«מ‬ÈÐÇÌփѬƙSZЊ֔ϒg»Ç¼ÐaB{‫®ײ‬¤ÇGzƭç‫׌‬ragϥϏgѧҥeɜKȉǌS`C|9ѧҥŉeFM|ÇÌփb«ÈÐgԱӛbԙъHXgÞӵdɷŢaB|bQ}`C|H‫®ײ‬¤ÇeFC`h̻‫ואה‬wƘЮg
¦Éʊуg֬eXgӚв։HǣǱS‫ײ‬B|ЮgµÊ¤ÐgΎʏԙъw¨ÈĈիe֛ØS`C|
ObĤzGbd]`C|9rZ‫»ײ‬ÇÇÌփhĸ}ZÉÐ¦ĕϧˀ^ObGz‫ײ‬ʂϥϏeFM|և֊ȷĀԠbg֛ØvОƮQ}`C|9
ʤ:h8‫ש‬ӑ Aspergillus oryzae eFM|»ÇÇÌփg͜Ҧ̂zGeU|oK‫ײ‬XgǗНЃdДӷɾ|Zue‫ײ‬՝ɓǖ‫̈ה‬gӑĒŉ»ÇÇÌփ։gѫ̈ǫŴ‫ײ‬rZ֊ȷ¦É‫ײ‬փŴ¦Éey|Ӛв։gǫůe^C`ԙoZ9֊ȷ ÌΞŧɼgӑĒŉ»ÇÇÌփ։gǫŴh֊ȷЮey]`ǱIKϴd{‫ײ‬Ӛв։HǣŧU|vgGz‫ײ‬pq͌ŐQ}dKd|pcΤȬU|vgra͔:aB]Z9rZ‫ײ‬թփŴʹѣʹ‫ײ‬B|Ch¿¨Ìey|փŴ¦ÉȉǌÖah‫ײ‬c\zgǞƙv»ÇÇÌփ։hǣŧU|ObĤzGbd]Z9̤ЗгgÒպh‫̤˻ײ‬ȋӨ˄ҺćЧȋЗгԳũʣì͏gũʣƐM`ӧ}Zvga
B|9Polyphosphate metabolism and stress response in Aspergillus oryzae
Sawaki Tada, Hikaru Ohkuchi, Ken-Ichi Kusumoto (NFRI, NARO)

P-38
Roles of AoLAH in regulating Woronin body position and function in Aspergillus oryzae
Pei HAN, Feng Jie JIN, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The Univ. ofTokyo)
Woronin body is a Pezizomycotina-specific organelle observed in the vicinity of septum, and plugs the septal
pore in response to cellular wounding. Previously, we characterized AoHex1 as the major protein of Woronin bodyin Aspergillus oryzae1), and investigated the Woronin body differentiation from the peroxisome2). Recently, theLeashin (LAH) protein was identified to tether Woronin bodies to the cell cortex in Neurospora crassa3). In thisstudy we attempted to investigate the roles of Leashin protein in regulating Woronin body position and function in A.
oryzae.
We predicted Aolah gene encoding a single polypeptide of 5,727 amino acids, which is homologous to the N.
crassa lah gene expressed into two distinct proteins3). AoLAH-EGFP expressed from the endogenous locus was
detected as Woronin body-like dots along the hyphae and closely associated with the septum. Transmission electron
microscopic analysis demonstrated that the Aolah gene disruption led to no tethering of Woronin bodies in thevicinity of septum. We also observed excessive loss of the cytoplasm upon hyphal injury in Aolah disruptant,suggesting the functional involvement of AoLAH in the septal plugging ability of Woronin body.
N-terminal conserved region of AoLAH (AoLAH[1-2039]) was expressed as EGFP fusion protein, and it was
detected at both sides of the septal pore, which is typical for Woronin body localization, suggesting a role of
AoLAH N-terminal region in association with Woronin body. C-terminal conserved region of AoLAH(AoLAH[4710-5727]) expressed as EGFP fusion protein was detected closely to the septal pore both in thewild-type strain and Aolah disruptant, indicating that AoLAH[4710-5727] itself functions for associating to theseptum. When AoLAH N- and C-terminal fusion fragment lacking the unconserved middle region was expressed in
Aolah disruptant, Woronin bodies were re-discovered in the vicinity of septum, however, the cytoplasm was still
excessively lost upon hyphal injury. This suggests that the unconserved middle region of AoLAH has a role in the
septal plugging ability of Woronin body, which is being further evaluated.
1) Maruyama et al. (2005) Biochem. Biophys. Res. Commun. Vol. 331, 1081-1088. 2) Escaño et al. (2009) Eukaryot.
Cell Vol. 8, 296-305. 3) Ng et al. (2009) PLoS Genet. Vol. 5, e1000521.

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FM|8ɳԺՆ˔‫׉‬ɢFyk DNA gś{Ցs8սœþŧ8ǫϴgȪŀdc8ȪŀgìԨeØE|ɶ‫׃‬e^C`8X}Y}gĥʀљg֛{ҖȡU|9The mode of random integration of exogenous DNA in Neurospora crassa
Ryuhei Kamada, Shuuitsu Tanaka, Shin Hatakeyama(Lab. Genet., Dept. Regulatory Biol., Saitama Univ.)(1Division Appl. Biol., Fac. Tex. Sci. Tech., Shinshu Univ., 2Mushroom Lab. Hokuto Co.)

P-40
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Analysis of the autophagy-related sterol glucosyltransferase AoAtg26 in Aspergillus oryzae
Takashi Kikuma, Takayuki Tadokoro, Katsuhiko Kitamoto(Dept. of Biotechnol., Univ. of Tokyo)- 58 —P-41tWÒ 
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Effects on growth and repression of A.oryzae GapA gene disruption.
Akihiro Kaneko, Hiromu Hamanaka, Motoaki Sano, Kenji Ozeki, Shinichi Ohashi(Kanazawa Institute of Technology)P-42‫ש‬ӑϩ̫˶Ӹ MOA ÉÐ‫מ‬Ͽϝ̼gĕӱbÊÌփϥϦҦgӽ̰ȹ̤йå‫ײ‬ȫŐʒҍϟ‫͉ײ‬ź̂‫ײ‬нΒԸƢ‫ײ‬Ǳа̢̫‫ײ‬ʉʹĺô‫ײ‬ŧӟְǨ ‫װ‬ƜǔǱÏՎ‫ױ‬ΟտgĀӪЃd‫ͳח‬ʣőaB|µÊÌփ¡Èwրփ ½ÈhցͯHſϔaϥƙʣaI|Zu‫ײ‬X}zgϥϦҦH‫מ‬CցͯgҡЮHЅeӧ}`C|9Ò˷‫ͳחײ‬ʣőgÜaΟտg‫ͳח‬ǣɰeևӵdÊ Ìփ¡Ègϥʣ͓͜e^C`h‫שײ‬ӑ Aspergillus oryzae ey{8яeƠr}|Ê ÌHÊÌփnbǫ˔Q}‫ײ‬Qzeցͯgĕϧey{8Ê Ìփ¡ÈHϥʣU|bǝƤQ}`C|H 1)‫֛ײ‬ØU|ցѣe^C`gДӷhdC9XOa̤ЗгahÊ ÌփgŜ‫י‬Ē 4-methyl-2-oxopentanoic acid
(MOA) Ê Ìփeǫ˔U|ցѣgˍѤӧ]Z9ӷŐSZցѣ‫מ‬ϿϝQWZ‫ש‬ӑĕӱU|Oba‫ח‬ƦԧGdƞօտgϥϦngȶ֙ev̠ɺQ}|9‫ש‬ӑg¬¾ʗǝGz MOA Ê Ìփeǫ˔U| 2-¦փ¥°§Ê¨Ð³½ÇÐeȷU| 5 ЮgղĈȆձŖSZ9X}zgղĈȆe^C`ǱҰӑțÞbS`Ѫ˔EցѣϴЮϿϝQW‫ײ‬єӱӧ]Zɼ‫ײ‬MOA ȉǌÖeFM| NAD(P)H gΤȬÀ©ÐU|Oba MOA É ÐΎʏg̚ςԙoZ9XgѬ̲‫ײ‬5 ЮgD\ 1 Юh NAD(P)H ȉǌÖa MOA Ê Ìփeǫ˔SZ9O
gղĈȆ TEF1 µÊÀÐÐÖaʐɓЃeթšϿϝU|yDe͓ьSZµÆ½§a‫שײ‬ӑɳԺՆ
˔SZ9ϝǌ‫ײ‬ĕӱSZ MOA É Ð‫מ‬Ͽϝ̼gÊ ÌփϥϦҦe^C`ӽ̰բu`C|91) Suzuki, M. Yoneyama, H. Koizumi, T. (1982) The mechanism of formation of ethyl leucinate, an importantaroma component in sake. Hakkokogaku, 60, 19-25.
Overexpression and characterization of a novel 4-methyl-2-oxopentanoic acid (MOA) reductase in Aspergillus
oryzae.
Tatsuya Yamamoto, Emiri Koide, Tiaki Mori, Takeura Kengo, Miki Daikoku Motoyuki, Shimizu, Masashi Kato
(Fac. of Agri., Meijo Univ.)- 59 —P-43 (O-9)јϒӑ Trichoderma reesei eFM|ӑĒǭ҇ѸϒϏԺƙʣցѣgˍѤϨƆċʓ 1‫˶ײ‬ϨҍԲȆ 2‫ײ‬ʉϨΌú 1‫ײ‬ǙɅТϥ 3‫̦ײ‬ȹΜƗ 3‫ײ‬Ǳ֨͢Ȇ 4‫ײ‬ȫоƆΡ 1
‫װ‬1 ֗ȻʮЧǱÏϥϏ‫ײ‬2 ЧȋʮӨ˄Һ͓͜‫ײ‬3 õռǱȋÏϥȉǇЗгʩ‫ײ‬4 ѷƙϭķЗгˢ˖‫ױ‬јϒӑ Trichoderma reesei hÈÊÐưÒgοѣΫbS`ǖ‫ה‬SZbIeǱ։gÈÆÐԒȪ
ϥϦU|9ÈÆÐϥϦ̪ăÖeFM|ӑјe^C`Լ̺ǒֹȆ‫׋‬ʂ֕ϧC`ӻȡSZbO~‫ײ‬ѧҥǤÕe҇ѸϒϏԺHϥϦQ}|bCDObĤzGbd]Z9rZ‫ײ‬ÈÆÐֿϥϦ̪ăÖavϏϟЃdřηƐMZbIeϥϦHġբQ}|ObHОƮQ}Z9SGSdHz‫҇ײ‬ѸϒϏԺgѪʣwϥϟȋЃdɷŢhŁKӽ̂Q}`CdC9āŜ‫ײ‬FT-IR ϧC`҇ѸϒϏԺgѪʣő̰ӧ]ZѬ̲‫҇ײ‬ѸϒϏԺ
H¡ÌaB|ƔҦʏHОƮQ}Z9¡ÌhјϒӑgѧҥǤgÞʣőgjb^aB{‫ײ‬јϒӑgɳʝeΛK֛]`C|Zu‫҇ײ‬ѸϒϏԺgϥϦb֛ՠHB|bҖEz}|9ϝǌ‫ײ‬T. reesei g¬¾¥Ð·Ðy{ӷC[SZ 6 Юg¡ÌƙʣցѣղĈȆgИǦӧC‫¡ײ‬Ìƙʣցѣb҇ѸϒϏԺbg֛ՠʏӽ̰S`C|9Ò˷‫ײ‬јϒӑgѧҥǤeȉǌU|Ǯˮg GPI ÌÐǒÌ¯ԺH‫҇ײ‬ѸϒϏԺgϥϦb֛ՠHB|bҖEz}ZZu‫ײ‬T. reesei g¬¾¥Ð·ÐGz GPI ÌÐǒÌ¯ԺղĈȆˍѤSZ9ӷC[Q}ZղĈȆe^C`‫ ¼ײ‬ÊÉ ӽ̰ϧC`‫҇ײ‬ѸϒϏԺgϥϦ̪ăbֿϥϦ̪ăaϿϝ˂ůgͱՈӽ̰ӧ]`C|9

Exploration of protein related to cell surface fibrous material synthesis of Trichoderma reesei
Shingo Tahara1, Mikiko Nitta2, Yosuke Shida1, Yoshiki Horikawa3, Junji Sugiyama3, Masako Osumi4, Wataru
Ogasawara1(1Nagaoka Univ. of Technol., 2JST, 3RISH, Kyoto Univ., 4IIRS)

P-44јϒӑg Poly(ADP-ribose) glycohydrolase gˍѤƊkXgĶI
ɗֈζ 1‫׆ײ‬ӟҍб 1‫̵ײ‬ȱĢú 2‫מײ‬ԦЊ͚ 2‫ײ‬ʉʹĺô 1‫ײ‬ŧӟְǨ 1 ‫װ‬1 ƜǔǱÏՎ‫ױ‬‫װ‬2 тΈǱ֤ÏϥƨϣǢ‫ױ‬»Ç(ADP-ÇºÐ) Ŵh‫ײ‬Ў̽ϥϏeϐϴЃdƔ՚ЃғԆɼĥ‫ד‬aB|9Ogƍʊeh‫»ײ‬Ç(ADP-ÇºÐ) ƙʣU| poly(ADP-ribose) polymerase (PARP) bőӽU| poly(ADP-ribose) glycohydrolase (PARG) g
2 Ю‫׌‬gցѣH֛ØU|ObHДz}`C|9PARP h‫ײ‬ցͯ֥KUo`gЎ̽ϥϏe‫מ‬ɢeģȉQ}‫ײ‬DNA ĥʀ‫ײ‬Նŋԙъ‫ײ‬ѧҥͦ‫ײ‬ÜʆĒgőӬŘɿe֛ØS`C|bҖEz}`C|9Aspergillus nidulans g¬¾Üev parp g ortholog H 1 ^ȉǌU|9Ò˷‫ײ‬ցͯFyk A. nidulans Ơtјϒӑg¬¾Üeh‫מײ‬сЎ̽ϥϏa‫מ‬ɢeģȉQ}`C| parg g ortholog hӷŐQ}`CdC9
XOa‫ײ‬A. nidulans g¬¾ÜGzªÉ¡§gĀԠe֛ØU|bҖEz}| 7 Ю‫׌‬g parg ĩӰղĈȆձʳSZ9ÇÌ±¨Ì¦Ì¯ԺԙӱS‫ײ‬ӽ̰SZѬ̲‫ײ‬1 Ю‫׌‬gÌ¯ԺH Mg2+ĜȉЃe»Ç(ADP-ÇºÐ) ŧʹőӽSZObGz‫ײ‬OgÌ¯Ժ fungal PARG (fPARG) bƜþMZ9Qze‫ײ‬A. nidulans e DNA ÈÈŴŞ(MMS) ΞŧSZbO~‫ײ‬parp bƛ͔e fparg gՆŋHΎʏŴQ}ZObGz‫ײ‬fPARG h DNA ˗Ĵʊуe֛ØU|ObHОƮQ}Z9ϝǌ‫ײ‬fparg ИǦ̼ĕӱS‫ײ‬ӽ̰S`C|9Identification and Characterization of the Novel Poly(ADP-ribose) Glycohydrolase of Filamentous Fungus
Aspergillus nidulans.
Mio Hirano1, Miho Sudo1, Shunsuke Masuo2, Naoki Takaya2, Motoyuki Shimizu1, Masashi Kato1 (1Fac. Of Agri.,Meijo Univ., 2Grad. Sch. of Life & Env. Of Tsukuba)- 60 —P-45‫ש‬ӑ Aspergillus oryzae ӑĒŉ֊ȷµÊ¤Ð ADAM g͜Ҧӽ̰ȫ̱ʻƸ‫ײ‬ŜϨΓ, нŉժ֯, ȹɳΌɗ ‫̭װ‬ՎɈǱ֤ÏʊϥŴ‫ױ‬? Ҥ ̍ F y k Љ Ѓ @ ‫ ש‬ӑ Aspergillus oryzae g µ Ê ¤  Ð  g Ü e h , ADAM ( A Disintegrin And
Metalloproteinase ) bgЋƛʏˀ^vgH 2 Ю‫׌‬ȉǌU|9ʤ:h, O}z 2 ^gµÊ¤ÐÐ§U|bˏȒQ}|ղĈȆ admA, admB bSZ9ADAM h, ֊ȷµÊ¤Ð§¿ Ìb¥ Ì¤ÇÌ§¿ Ìˀ^Ì¯ԺaB{, ADAM ³½ÇÐbS` 30 ԾE|¿Ì®ÐHȉǌU|9
ADAM g͜Ҧh, ϐe°¦w¼aǮKgǝƤHB{, ѧҥˎАwÌ¯ԺµÊ¢Ì, ¨ÈĈիdce֛ØU|ObHДz}`C|9Ўӑg ADAM ah, A. fumigatus gѪ˔Eǒ ADM-B H
֊ȷµÊ¤ÐΎʏ̚S sheddase bS`͜ҦSɾ|ObwőӬցͯ Schizosaccharomyces pombe g
Mde10 HҥȆӪȸ͓՟g͓ьeĶKbˏȒQ}`C|vgg, ЎӑagϥϏȋЃd͜ҦĥzGed]`CdC9XOa̤Згah, ‫ש‬ӑ A. oryzae eFM| ADAM g͜Ҧ̂zGeU|ObЉЃbSZ9?˷·FykѬ̲@ admA, admB ղĈȆИǦ̼ ∆admA ̼b ∆admB ̼, Qze ∆admA∆admB ̼ĕӱ
S8ȟǲɗ̯ǖ‫ה‬agӪϝǒӻȡӧ]Z9Ì¦ÊÐÈ̼bƘИǦ̼ah, ̘ȫǖǎeFM|ϥҡgɋhԐuz}dG]ZH, calcofluor white vSKh congo red ΞŧSZǖǎahӅɖgʜƐʏОS, Q
ze 371 aOgʜƐʏHǣŧSZ9OgObGz, ղĈȆИǦey{ѧҥǤ¦ÉʊуeǫŴHϥT
ZbҖEz}|9rZ, Լ̺ǒֹȆ‫׋‬ʂ֕ey{őϥȆӻȡSZbO~ИǦ̼gőϥȆhőϥȆӪ‫׀‬g
͓՟HÌ¦ÊÐÈ̼bϴd]`CZ9OgѬ̲Gz, ‫ש‬ӑ A. oryzae g ADAM hőϥȆgʣψe֛Ø
U|ObHОƮQ}Z9
Functional analysis of intracellular metalloprotease ADAM in Aspergillus oryzae
Takuji KOBAYASHI, Hiroshi MAEDA, Michio TAKEUCHI, Youhei YAMAGATA(Dept. of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology)

P-46‫ש‬ӑHϦϥU| hydrophobin RolA bǄĒӪ‫֚׀‬gЋîĕϧe֛U|ЗгϨÜʻ̢ 1‫ײ‬Ϩշɬͮ 1‫͛מײ‬ʅ 2‫ײ‬ō͙Բʣ 3‫̚ײ‬ϨЭɴ 4‫֡ײ‬պ˭ʕ 1,2‫װ‬1 ̭ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ײ‬2̭ŵǱÏ̢̫З‫ײ‬3 ȹɳǱϟÏϏԺϥƨŴȋ‫ײ‬4 ̭ŵǱÏǮĺЗ‫ױ‬ ‫ש‬ӑgϦϥU|ÚӺȄʏӣЁԺ hydrophobin RolA hϥőӽʏ»Ç¤È PBSA eơАS‫ײ‬PBSA őӽցѣ CutL1 ey| PBSA őӽġբU|9Ò˷‫ײ‬ǄĒӪ‫׀‬b hydrophobin ֚gЋîĕϧ͓͜w‫ײ‬Xg kinetics
h×̂aB|9O}rae‫ײ‬3RolA g Leu137, Leu142 H PBSA ngѬƙeևӵ‫ײ‬4pH4-10 ag PBSA n
g RolA gӺƩʏh pH4 a̘Ǳ‫ײ‬5PBSA ʂђȆg pH4-10 agÐֹčhÜʏaԪ‫ײ‬փʏa 0 bd|Ѭ̲ɾZ9āÕgѬ̲Ɗk‫ײ‬RolA g pI=4.6-4.8 bCDѬ̲Gz‫ײ‬PBSA ng RolA ơАeFC`/϶ʹЃЋîĕϧ0H͢e‫ײ‬/ԪֹӍg‫ֹ־‬ƍϿ0HԪeĶKƔҦʏHˏȡQ}Z9̤ǝƤh‫͔ײ‬:dǄĒӪ‫׀‬ng RolA ơА͔ɩgӽ̰ЉЃbS‫ײ‬ϴd|ŴȋЃʏԺþØSZӪ‫׀‬ng RolA ơА‫ײ‬QCM ϧC`
pH4, 7, 10 aԉĝSZ9 ϶ʹɢHϴd|Ӫ‫׀‬eȣU| RolA gӺƩʏh‫ײ‬Ӫ‫׀‬g϶ʹɢbЋ֛ʏОSZ9ֹ͢ӍӪ‫׀‬eȣU|ӺƩʏh pH4, 7 aςӍֹʏӪ‫׀‬y{Õ̀S‫ײ‬pH10 aĎÖSZ9Ò˷‫ײ‬ԪֹӍӪ‫׀‬eȣS`h RolA HÒȒιɢāÖgǞƙeơАHpbcӷz}dG]Z9āÕGz‫ײ‬ǄĒӪ‫׀‬ng RolA gӺƩʏeFC`‫ײ‬ǄĒӪ‫׀‬g϶ʹʏƊkֹ͢ӍHơАe‫ײ‬ԪֹӍHƍϿeĶKƔҦʏHОƮQ}Z9rZ RolA h pH4 aǄĒӪ‫׀‬e‫מ‬CӺƩʏОS‫ײ‬RolA ιɢÕ̀eĊDʎηdơА։ǣŧHЛԐQ}Z9RolA hÚӺȄʏaB{‫ײ‬Ӎֹʏ½¬փeȞt‫ׇ‬Ǖb϶ʹʏ½¬փeȞt‫ׇ‬Ǖˀ^ObGz‫ײ‬ǄĒӪ‫׀‬gʏԺeĜȉS`RolA gˎՔսƝʏwˎǎսƝʏHϴd{‫ײ‬kinetics Hɶ‫׃‬ƐM`C|ƔҦʏHˏȡQ}|9Analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces
Takumi Tanaka1, Hiroki Tanabe1, Toru Takahashi2, Takanari Togashi3, Toshihiko Arita4, Keietsu Abe1,2 (1 Grad. Sch.
Agric. Sci., Tohoku Univ., 2 NICHe., Tohoku Univ., 3 Dept. Sci., Yamagata Univ., 4 IMRAM., Tohoku Univ.)- 61 —P-47‫ש‬ӑ¸µ¡ÌֿʜƐʏµÊ¤ÐgցѣȋЃʏԺgӽ̰нŉЎϟө8Ȼ̤ѻȆ8֡ģ̅Ӂ8ŜϨΓ8ȹɳΌɗ8нŉժ֯ ‫װ‬ՎɈǱ֤ÏʊϥŴ‫ױ‬ ‫ש‬ӑ¬¾ӽ̰gѬ̲Gz8Aspergillus oryzae h 3 ^g pepstatin insensitive protease (PIP) ղĈȆ‫װ‬317-0628
315-0058306-018‫ױ‬ˀ^ObĤzGed]Z9PIP bhփʏ‫ׇ‬ǕaĕϧU|Ì§ǒµÊ¤Ða
B{8DGDT W(F/Y)EW(F/Y/L)P ED(F/Y) gΎʏÜʆÀ¡Ð³HӷŐQ}`C|9PIP h8aspartic
protease bhլC8¸µ¡Ìey]`Ύʏ֠ȘƐMV8È½ÌփͩǗHӾȄͩǗaB|ObG
z8glutamic protease bCD˶SC¤ÇÐeő‫׌‬Q}`C|9̤Згah A. oryzae g PIP gցѣȋЃʏԺgӽ̂ЉЃbS8ӽ̰ӧ]Z9
 PIP ղĈȆgϿϝϒ΄e^C`ЛԐSZʩ8ǖ‫̪ה‬ăey]` 3 ^Ł`H mRNA bS`ϿϝS`C|ObHŕ̂SZ9rZ8ցѣȋЃʏԺgӽ̰ӧDZue8317-0628315-005 h Pichia pastoris8306-018 h
Aspergillus nidulans țÞbSZϿϝљϧC`8ϿϝSZ9317-062 b 306-018 hƔέʏϭőeϿϝSZ9
Ò˷8315-005 h×έʏϭőeϿϝSZZu88 M urea ey|ƔέŴb8֪ͫ՛̰·ey|Ç³ÐÈ¥Ìӧ]Z9єӱSZ 3 ^g PIP h8փʏ pH ‫ׇ‬ǕeFC`8¸µ¡Ìe֠ȘQ}dCÌ¯ԺǗԺeȣU|ΎʏОSZ9317-0628306-018 e^C`h8Ҹկ pH8pH ȎȒʏdcgӽ̰ӧ]Z9
ϝǌ8315-005 e^C`8̘կdÇ³ÐÈ¥Ì̪ăb8ԎѧdցѣȋЃʏԺ͌ԁS`C|9
Enzymatic Properties of Pepstatin Insensitive Protease in Aspergillus oryzae
Marii Takeuchi, Ayako Okamoto, Haruka Abo, Hiroshi Maeda, Youhei Yamagata, Michio Takeuchi(Division of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology)

P-48 (O-10)
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ОUH‫ײ‬XgиĒ͓՟wǗԺԐԢ͓͜ĥzGeQ}`CdC9 ̤Згah‫ײ‬¹ÀÊÐÀ¥ÇÌgʪ·ϧC` AAC gиĒ͓՟À¥ÈĕӱSZ9OgÀ¥Èb'Æ¾ÆÐgѬ͓̎՟ϧC`‫ײ‬ÉÌ A ƠtƘЮǗԺbg§¢ÌԀцӧ]Z9XgѬ̲‫ײ‬AAC gΎʏպčeh‫'ײ‬Æ¾ÆÐehςC‫ײ‬ÉÌ A g֗CÈĮ֔ԐԢU|ѧ֗CѬƙ»¢¦HȉǌU|ObHОƮQ}Z9OgÀ¥È͌ԈS‫ײ‬ÁÌ¥ÌљʲϥϏԺgϥϦeŗϧŐ̫|˶ӸցѣgƏɾЉЃbS`‫'ײ‬Æ¾ÆÐgÒ^aB|³Ê»ÇÌÆÐ‫װ‬CA‫ױ‬gΎʏպčeӲˮg½¬փҋ˔ȪŀS‫ײ‬ΛCÈǗѬƙ»¢¦v^ǫϴĒցѣԄԀSZ9ǫϴ PCR gʪ·ϧC`‫ײ‬Pseudomonas sp. SY-77 ϩ̫ CA gǫϴĒղĈȆĕ
ӱS‫ײ‬ǱҰӑgϿϝљŗϧS`ǫϴĒցѣƙʣSZ9єӱSZǫϴĒցѣh‫ײ‬ֈϥǒցѣbՈo`‫ײ‬³Ê»ÇÌeȣU|ΎʏHĎÖSZÒ˷‫ײ‬ÉÌ A eȣU|ΎʏH‫׋‬ӘeǣǱSZ9O}zgѬ̲h‫ײ‬AAC b'Æ¾ÆÐgǗԺԐԢ͓͜gլCӽ̂S‫̤ײ‬ЗгaϧCZÀ¥ÇÌb§¢Ìgʪ·H8ցѣgǫϴĒԄԀe̚ŬaB|ObОU9
Substrate switching of antibiotics acylase by 3D modeling
Kazuki Nakayama, Masato Yamada, Hiromi Nishida, Yasuhiro Isogai(Toyama Pref. Univ., Dept. Biotech.)- 62 —P-49‫ש‬ӑgŎçցѣg͜Ҧӽ̰Ϩà ֧Ȇ‫ײ‬Ȼ̤ ѻȆ‫ײ‬ŜϨ Γ‫ײ‬ȹɳ Όɗ‫ײ‬нŉ ժ֯ ‫װ‬ՎɈǱ֤ÏʊϥŴ‫ױ‬?ЉЃ@Ŏçցѣbh¡Ðgӱ՟թЬaŎǄŞbS`ĘϧQ}|ցѣa‫ײ‬ƓKGzûώgп 4 ңGzʸŐSZ chymosin HĘϧQ}`IZ9chymosin hçÜeƠr}|*- casein g Phe105 – Met106 ϐϴЃeőӽU|Oba‫ ײ‬Ì½ÈHɂǦS‫ײ‬Ð§ϥʣU|9Aspergillus oryzae ahŎçցѣgȉǌHǝƤQ}`C|9SGS‫ײ‬XgցѣÐ§U|ղĈȆbցѣÌ¯g֛Ġe^C`ĥzGed]`
CdC9¬¾ӽ̰gѬ̲‫ײ‬A. oryzae RIB40 h 11 Ю‫׌‬g¯È¤¢µÊ¤ÐղĈȆģ̚S`C|ObĤzGbd]`C|9̤Згah‫ײ‬O}zgµÊ¤ÐղĈȆϦϏe^C`ŎçΎʏgÇÐ©ÌӧC‫ײ‬ցѣŴȋЃʏԺƛȒU|ObЉЃbSZ9?˷·FykѬ̲@Ƙ¯È¤¢µÊ¤ÐղĈȆϿϝSZÇÐ©ÌgѬ̲‫ײ‬1 ^gղĈȆϦϏa‫מ‬CŎçΎʏHЛԐQ}‫ײ‬X} AOchymosin 1 bƨƜSZ9A. nidulans A89 ¹¦e
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Characterization of milk clotting enzymes in Aspergilli
Yoko Takyu, Ayako Okamoto, Hiroshi Maeda, Yohei Yamadata, Michio Takeuchi( Division of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology )P-50
Aureobasidium pullulans eϩ̫U|Ȏʑ‫ח‬փ-4-ʹփŴցѣղĈȆgӽ̰̭ϨДΌ‫ײ‬ǳϨÒҾ ‫װ‬ȖɁǱՎÏʊϥЧ‫ױ‬?ЉЃ@íɳʏЎӑ Aureobasidium pullulans ATCC 20524 ̼Gz‫˺ײ‬eÆ±¬ÆÌőӽe֛ØU|ցѣ&-L-Æ±¬³Æ¬ ÐÐ§U|ղĈȆ‫װ‬abfB‫ױ‬Ơt DNA ˴ό‫װ‬4,996 bp‫ױ‬ÊÐ©Ì
SZ9Og DNA ˴όg¹ÀÊÐ͌ѤgѬ̲‫ײ‬abfB ղĈȆgÖΏeȎʑ‫ח‬փ-4-ʹփŴցѣÐ§U|
ղĈȆ(bphA)bˏȒQ}|սœӷŐSZ9̤ǝah‫ײ‬A. pullulans ATCC 20524 ̼Gz cDNA ԙӱS‫ײ‬RACE (Rapid Amplification of cDNA Ends) ·ey{ bphA ղĈȆg‫ׇ‬ǕϐȒS‫ײ‬ӽ̰U|ObЉЃbS
Z9?˷·ÏѬ̲@A. pullulans ATCC 20524 ̼®̧ϩ̫ÆÌοѣΫbS` 301a 3 ˻֚ǖ‫ה‬SZ9
XgӑĒGz mRNA ʸŐS‫ײ‬cDNA Æ ´ÆÇÐĕʣSZ9Xg cDNA ֐ǒbS` RACE ·ey{ 5 RACE µÆ ¼ÐrZh 3 RACE µÆ ¼Ðb‫ײ‬bphA ղĈȆeϐϴЃdµÆ ¼ÐϧC` PCR
aǣɔSZ9˺Дg abfB ղĈȆgѩ͡§ÌGz 555 bp ÖΏe bphA ղĈȆg ORF‫װ‬1,512 bp‫ױ‬HȉǌS‫ײ‬
54 bp g Ì¦ÊÌH 1 ÎʩúǌSZ9rZ‫ײ‬bphA ղĈȆg֙ǿ§ÌGz 96 bp ÕΏe TATA º¢‫ײ‬
63 bp ÕΏeՆŋ֙ǿπ‫ײ‬ѩ͡§ÌGz 114 bp ÖΏe»Ç A þŧպčHȉǌSZ9bphA ղĈȆehőΆ¨È¸µ¡§HȉǌSdCObGzѧҥŉeȉǌS‫ײ‬ÆÌőӽeĊCϦϥQ}|³ÈÆփсgӂ‫ח‬ϣ̚U|ŴƙϏςͰŴU|͜ҦHˏΧQ}Z9͟e bphA cDNA gŁ ORF  EcoRI b NotI պčþŧSZµÆ ¼ÐaǣɔS‫ײ‬Ř֣ցѣΘŴɼ pET-26b ·Ðe˅ŀSZ9͓ьSZµÆ½§
pBPH103 h E. coli BL21 (DE3) ̼eȪŀSZ9ùɼ‫ײ‬bphA cDNA ϿϝQW‫ײ‬XgѪ˔Eցѣ BphA gցѣŴȋЃԝʏԺ͌ԁU|9̤Згh˱պЧȋЌϐŖ˪ҡЗгѫԳЗгˏբì͏gÒϣbS`ӧ}Z9Characterization of benzoate-4-hydroxylase gene from Aureobasidium pullulans.
Chihiro Higashida, Kazuyoshi Ohta
(Dept. Biochem. Appl. Biosci., Univ. of Miyazaki)- 63 —P-51
Aspergillus nidulans g GfsA h O-ÇÌg Galƒ ƙʣU|ѕՆЫցѣaB|
ĺ̮ծ 1‫ײ‬íСΊǗ 2‫ײ‬ΕֈǍՊ 1‫ײ‬нɅӝ 2‫ײ‬ɼӟ͢ŗ 2‫ײ‬ֈ̨ƴə 1‫ײ‬Ȼʻí 1 ‫װ‬1 ɀǔǱȋÏϥϏϥƨÏ
ʊʂɈ82 äǱÏ֤ÏՎ‫ױ‬ʤ:h, O}rae Aspergillus nidulans g GfsA HÆ¦³Æ¬Ð(Galƒ)ՆЫցѣaB|ObǝƤS`IZ9ùƾ, GfsA H O-ÇÌgֿյḷ́лeȉǌU| Galƒ ƙʣU|ցѣaB|Ob̂zGeSZgaǝƤU|9∆gfsA y{ʸŐSZÆ¦¼Ì¬µÊ¤ Ì(GMP)ƐȚǗԺbS`, єӱSZ
GfsA-3xFLAG Fyk UDP-Galƒ bŅeƍʊQWZɼe, EB-A2 ey]`Ì´Ê¢¦ӧDb
40-200 kDa gыǂa¨ÈH͌ŐQ}|9Og¨Ègϩ̫ԙo|Zue GMP  PNGase F ŏϟv
SKh'-elimination ŏϟSZ9XgѬ̲, PNGase F ŏϟah¨Èɰɢeɶ‫׃‬ƐMdG]ZH'-elimination ŏϟah¨ÈɰɢHΤȬSZ9rZ, O-ÇÌgƙʣ֙ǿʹD pmtA Fyk pmtC ղĈȆgИǦ̼GzʸŐSZ GMP ah wt eͱo`¨ÈHΤȬS`CZ9͟e, O-ÇÈŴÌ¯
ԺaB| WscA-HA ϿϝU| wt, ∆gfsA X}Y}͓ьS, Ƙ̼y{ WscA-HA єӱSZ9X}Y}
HA ʲĒb EB-A2 ϧC`ӽ̰SZ9XgѬ̲, ∆gfsA ϩ̫ WscA-HA g EB-A2 ey|¨ÈɰɢHΤ
ȬU|ObĤzGed]Z9Qze, ∆gfsA y{єӱSZ WscA-HA ƐȚǗԺbS`єӱ GfsA-3xFLAGϧC` in vitro Galƒ ƙʣցѣΎʏ͌ŐSZbO~, WscA-HA Õg Galƒ ͩǗH͌ŐQ}Z9āÕgOb
Gz, GfsA H O-ÇÌgֿյḷ́лeȉǌU| Galƒ ƙʣU|ցѣaB|ObĤzGed]Z9
GfsA is a novel glycosyltransferase involved in biosynyhesis of galactofuranose antigen of O-glycan inAspergillus nidulans.
Haruka Motomatsu1, Taiki Futagami2,Keisuke Ekino1, Kaoru Takegawa2,Masatoshi Goto2,Yoshiyuki
Nomura1,Takuji Oka1(1 Univ. of Sojo,2Univ. of Kyusyu)

P-52°ÆgÇ»¨ÐղĈȆg͜Ҧӽ̰ȫ̱Ǳԃ‫ײ‬ȫ‫׬‬ĭǳ‫ײ‬Ϩ‫׮‬ӯí ‫֗װ‬Ȼ‫מ‬ȥÏϏԺɈ‫ױ‬؆ЉЃ؇¬gĀӪЃd‫ͳח‬ʣő 1-¤Ì-3-ÐÈh‫ײ‬Ç¬ÐÈփGz°§Ê¸ÈÇ¬ÐÈփѫ`ƙʣQ}|bˏȒQ}`C|9Ç»¨Ð‫װ‬LOX‫ױ‬hÇ¬ÐÈփgփŴe֛ØS`C|bҖ
Ez}`C|9°Æ N001 ̼g¬¾¥Ð·ÐϧCZÀ¡Ð³͌ѤgѬ̲‫ ײ‬2 ^gˏȒ LOX
ղĈȆ‫װ‬PoLOX1‫ײ‬PoLOX2‫ױ‬HЛԐQ}Z9̤Згah‫ײ‬°Æg LOX g͜Ҧ̂zGeU|Zu‫ײ‬PoLOX1 b PoLOX2 g cDNA gǡǗսœͻȒSZɼ‫ײ‬ǱҰӑaϿϝQWZ PoLOX1 b PoLOX2 gϥŴȋЃʏԺԙoZ9؆˷·bѬ̲؇ƘЮ PCR ·ey{‫ײ‬PoLOX1 b PoLOX2 g cDNA ǣɔSZɼ‫ײ‬ǡǗսœͻȒSZ9
PoLOX1 b PoLOX2 h 640‫װ‬őȆ։ 72 kDa‫ױ‬b 661‫װ‬74 kDa‫ױ‬g½¬փGz͓ʣQ}|ˏȒÌ¯ԺÐ§S`F{‫ ײ‬Ì¦ÊÌ 1 ^ƠaCZ9PoLOX1 b PoLOX2 gÐ§‫ׇ‬Ǖg cDNA  pET16b·ÐeՠѬɼ‫ײ‬ǱҰӑ BL21(DE3)̼eɳԺՆ˔SZ9ɳԺՆ˔ĒʢθΙg SDS-PAGE gѬ̲‫ײ‬ëΧQ}ZőȆ։g 72 kDa b 74 kDa e¿ÁÐd®Ì§HЛԐQ}Z9Ҹկ pH ԙoZbO~‫ײ‬PoLOX1h pH 11.5‫ײ‬PoLOX2 h pH 5.5‫ײ‬7.0 a‫מ‬CΎʏОSZ9rZ‫ײ‬PoLOX1 b PoLOX2 g Km‫ײ‬Vmax h‫ײ‬X}Y} 0.05 mM‫ײ‬0.22 µmol/min Fyk 0.18 mM‫ײ‬0.90 µmol/min aB]Z9Functional analysis of two lipoxygenase genes of Pleurotus ostreatus strain N001
Daiki Kobayashi, Kenta Oguro, Yuji Tasaki(Dept. of Mater. Eng., Nagaoka Col. of Technol.)- 64 —P-53½¬ÇΎʏƊkǼǡʏОU‫ש‬ӑ Aspergillus oryzae g D-½¬փϐϴЃ½¬¸µ¡ Ð(DamA)gӽ̰̮Ö‫͉װ‬Ϩ‫ױ‬Ўϩҍ 1‫ײ‬ÜɅƀá 1‫ײ‬ǮϨŦϥ 1‫ײ‬ÝðΜÒ̝ 1, 5‫̡֋ײ‬Ҝ 1‫̜ײ‬պ‫ׇ‬ǳ 1‫ײ‬ǲֈø 2‫ײ‬ЖϨƀ͚ 3‫ײ‬ȹɳΌɗ 4‫ײ‬нŉժ֯ 4‫̤͎ײ‬ʡÒ 1‫װ‬1 ՎЗ͓͜Ï‫׏‬ѼЗ‫ײ‬2 ǲֈÌ¾‫ײ‬3 ̙́Ō‫ײ‬4 ̭õՎɈǱÏ֤‫ײ‬5JIRCAS‫ױ‬Ҥ̍@®¤Çgβ-½¬¸µ¡ Ð(BapA)h‫¸ײ‬µ¡§֔g N ̣лgβ-½¬փ(β-Ala с)ϐϴЃeԐԢU|ցѣaB{‫¬½ײ‬ÇΎʏ̚S`C|(Heck, T et, al. 2007, 2012)9ʤ:h BapA eЋƛʏОUղĈȆ (AO090138000075) Aspergillus oryzae ¬¾¥Ð·Ðy{ӷŐS‫ײ‬Xg͜Ҧe^C`ӽ̰ӧ]Z9˷·bѬ̲@A. oryzae țÞbS`‫ײ‬ÕԃղĈȆgթšϿϝ̼ N ̣лe His-Tag 
þŧU|ԄԀaĕʣɼ‫ײ‬ςѧҥʸŐΙy{єӱSZ9єӱցѣhɏԮg 17 Ю(L Ē 13 Ю, D Ē 3 Ю, βĒ 1Ю)g aminoacyl-p-nitroanilide (pNA)ǗԺeȣS‫ ײ‬β-Ala-pNA Ǽt BapA bhϴd{‫ ײ‬D-Leu-pNA b
D-Phe-pNA eȣS`‫מ‬CǗԺϐϴʏОSZZu‫ײ‬D-stereoselective aminopeptidase (DamA)bƜþMZ9͟
e D-Leu-NH2  Ǘ Ժ e S ‫ ײ‬ƍ ʊ Ϧ Ϗ  LC/MS/MS  ϧ C ` ӽ ̰ U | b ‫ ײ‬D-Leu-OH [ M a d K
D-Leu-D-Leu-NH2 H͌ŐQ}‫ײ‬DamA v BapA ƛ͔½¬ÇΎʏ̚U|ìĤzGbd]Z9rZ
D-Leu-pNA ǗԺeS`‫ײ‬ƍʊΙe 3.0 M NaCl ŧE|b‫ײ‬ȣφbͱՈS` DamA g pNA զֵΎʏh 3.1
ĨeÕ̀SZ9Qze‫ײ‬DamA g½¬ÇΎʏϧC`‫ײ‬CK^GgϥϟΎʏ¸µ¡§gƙʣHЛԐŐ̫Z9 ̤ЗгgÒպhϥЗÌÐǗНЗгˏբì͏ey{Ȕ˸Q}Z9M. Matsushita-Morita et al.
Appl. Biochem. Biotechnol. (in press)
Characterization of a D-stereoselective aminopeptidase (DamA) from Aspergillus oryzae
Mayumi Matsushita-Morita1, Hiroyuki Nakagawa1, Sawaki Tada1, Junichiro Marui1,5, Satoshi Suzuki1, Ryota
Hattori1, Hitoshi Amano 2, Hiroki Ishida3, Youhei Yamagata4, Michio Takeuchi4, Ken-Ichi Kusumoto1(1 Natl. Food Res. Inst., NARO, 2Amano Enzyme, 3Gekkeikan, 4Tokyo Univ. of Agric. Tech., 5 JIRCAS.)

P-54‫ש‬ӑ Aspergillus oryzae pepO ղĈȆ͞˗̼gӑĒǭÌ§ǒµÊ¤Ðe^C`ÕֈѯȆ‫ײ‬ŜϨΓ‫ײ‬ȹɳΌɗ‫ײ‬нŉժ֯‫װ‬ՎɈǱ֤ÏՎ‫ױ‬; ЉЃ <¬¾ӽ̰gѬ̲8‫ש׫‬ӑ Aspergillus oryzae RIB 40 ehµÊ¤ÐÐ§U|bˏȒQ}|ղĈȆH 126 ħȉǌU|ObĤzGed]Z9āŜgǝƤa8RIB 40 gӑĒǭeFC`փʏ̪ăÖaĕϧS¸µ¡Ìa֠ȘQ}|¯È¤¢µÊ¤Ð‫ װ‬APase ‫ױ‬h PepO gsaB|ObH
ОQ}Z9XOa8̤Згah8pepO  pyrG aҋ˔SZ pepO ղĈȆ͞˗̼ĕӱS8Xg͞˗HýgӑĒǭőΆgփʏÌ§ǒµÊ¤ÐeƊqUɶ‫׃‬e^C`ԙo|ObЉЃbSZ9; ˷·ÏѬ̲ <pepO ղĈȆ͞˗̼‫װ‬%pepO ‫ױ‬ĕӱSZ9%pepO gǖ‫ה‬ÕΟe^C`8փʏ̪ăÖaĕϧU|Ì§ǒµÊ¤ÐgΎʏΧȒӧ]ZbO~8ֈϥǒg RIB 40 eͱoћ 5 ŢЬɢgΎʏHԐuz}Z9OgΎʏh¸µ¡Ìey|֠ȘƐMV8APase ahdCObĤzGed]Z9X
Oa8%pepO gǖ‫ה‬ÕΟeɛЮGgµÊ¤Ð֠ȘŞΞŧS`ΎʏΧȒSZѬ̲8antipain ey{ɰ K ֠ Ș Q } Z 9 antipain e ֠ Ș Q } | փ ʏ  Ì § ǒ µ Ê ¤  Ð  a B | AorsinA g Ǘ Ժ a B |Z-Arg-Arg-MCA ϧC`ΎʏΧȒӧDb8ΎʏHԐuz}Z9ֈϥ̼ RIB 40 b%pepO eFM| aorsinAղĈȆgՆŋ։eǫŴhԐuz}dG]Z9āÕy{8RIB 40 gӑĒǭeFC`փʏ̪ăÖaĕϧU|ÞdÌ§ǒµÊ¤Ðh PepO b AorsinA aB|ObHОƮQ}Z9
Extracellular endoproteases of Aspergillus oryzae pepO gene deletion strain
Ayako UENO , Hiroshi MAEDA , Youhei YAMAGATA , Michio TAKEUCHI(Division of Applied Biological Chemistry,Tokyo Univ. of Agriculture and Technology)- 65 —P-55
Aspergillus oryzae gӑĒŉ֊ȷµÊ¤Ð saccharolysin homolog g͜Ҧӽ̰ЖɅ ֯Ò̝‫ײ‬ŜϨ Γ‫ײ‬нŉ ժ֯‫ײ‬ȹɳ Όɗ ( ՎɈǱ֤ÏՎ )[ЉЃ] A. oryzae g¬¾ehǮKgµÊ¤ÐղĈȆHȉǌS`C|9½¬փսœgЋƛʏGz‫ײ‬S.
cerevisiae e h 1 ^ S G ȉ ǌ S d C saccharolysin g homolog   Ð § U | ղ Ĉ Ȇ H ӷ Ő Q } ‫ײ‬oryzalysin ( Ols ) A , B , C bSZ9Saccharolysin h‫¦½ײ‬Ì§ÇҲ֚ҭeȳǌU|֊ȷ¸µ¡ Ða‫¦½ײ‬Ì§ÇҲ֚ҭw¼¦Ç¢֚aփŴ ¿ÐƐMZÌ¯ԺHőӽQ}`ϦϥQ}|¸µ¡§w‫¸ײ‬µ¡ Ðey]`Œ˴Q}Z¨È¸µ¡§őӽU|͜ҦHցͯeFC`Дz}`
C|9ցͯeͱS`‫ ײ‬A. oryzae ¬¾Üeh‫ ײ‬ols A , ols B , ols C g 3 ^gղĈȆHȉǌS`C|9O
gObGz A. oryzae ah Ols g͜ҦőʹgƔҦʏHОƮQ}Z9̤Згah‫ ײ‬A. oryzae eFM|
oryzalysin g͜Ҧӽ̰ЉЃbSZ9[˷·ÏѬ̲] ols A , ols B , ols C gƘղĈȆgſϔИǦ̼‫ײ‬թšϿϝ̼ĕӱS‫ײ‬Ì¦ÊÐÈ̼bͱՈS
Z9ols A , ols B , ols C gƘſϔИǦ̼h‫ײ‬ҪŅɷŞ 2 , 4 – dinitrophenol ey]`½¦Ì§ÇҲֹčΘǶQWZ̈‫ײ‬Ì¦ÊÐÈ̼y{Ê©ÐÜʆպeFM|ͳÜӑјgċ֗ġբHӷz}Z9olsA թšϿϝ̼h‫ײ‬olsA HթšeϿϝU|̈‫ײ‬Ì¦ÊÐÈ̼y{Ê©ÐЊɹ‫ײ‬őϥȆАϥgΤȬHӷz}
Z9ols A , ols B , ols C gƘղĈȆgſϔИǦ̼‫ײ‬թšϿϝ̼gՆŋӽ̰ӧ]Z9olsC ИǦ̼eFC`
olsA gՆŋ։HΤȬS‫ײ‬olsC թšϿϝ̼eFC` olsA gՆŋ։HǣŧS`CZ9OgObGz olsA b
olsC gՆŋÉ·ÈagŽԙʏHОƮQ}Z9ϝǌ‫ײ‬Ì¯ԺÉ·ÈeFM|ӽ̰ӧ]`C|9
Functional analysis of intracellular metalloproteases saccharolysin homolog in Aspergillus oryzae
Yuichiro ISHIKAWA,Hiroshi MAEDA,Michio TAKEUCHI,Youhei YAMAGATA(Division of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology)

P-56 (O-7)
Trichoderma reesei eȉǌU| 10 Ю‫׌‬gβ-glucosidase isozyme gցѣŴȋЃʏԺջ ƀΌ‫ײ‬ֈ‫׮‬ŦÒ‫ʹײ‬ֈ͢Γ‫ײ‬ǲֈҾɴ‫װ‬ĤɆǱ֤Ïϥƨ͜ҦÏ³®ÐɈȋ‫ױ‬јϒӑ Trichoderma reesei gÈÆÐԒȪϏԺaB|¹ÊÐh‫ײ‬β-glucosidase (BGL) ey|ѕՆЫƍʊey]`ƙʣQ}|ObHˏȒQ}`C|9Xgƙʣe֛ØU| BGL ϐȒU|Zue‫̤ײ‬ӑeȉǌU| 10 Ю‫׌‬g BGL (Cel1A‫ײ‬Cel1B‫ײ‬Cel3A‫ײ‬Cel3B‫ײ‬Cel3C‫ײ‬Cel3D‫ײ‬Cel3E‫ײ‬Cel3F‫ײ‬Cel3G Fyk Cel3H)
e^C`Ͽϝљ͓ьSZ9O}zѪ˔EցѣgєӱӧC‫ײ‬ѕՆЫΎʏƠuZցѣŴȋЃʏԺԙ̺
SZ9
GH1 eȷU Cel1A Fyk Cel1B hǱҰӑeFC`Ͽϝљ͓ьS‫ײ‬Xgý GH3 g BGL h‫שײ‬ӑeF
C`Ͽϝљ͓ьSZ9O}zgD\‫׽ײ‬Ю‫׌‬g BGL hCV}v p-Nitrophenyl-β-D-glucoside (pNPG1) bÊ±Ð (G2) eȣS`őӽΎʏОSZH‫ײ‬Cel3H eh BGL ΎʏHԐuz}dG]Z9BGL ΎʏОSZ‫׽‬Ю‫׌‬gցѣe^C`‫ײ‬pNPG1 b G2 eȣU|őӽΎʏͱՈSZbO~‫ײ‬Cel3A‫ײ‬Cel3B Fy
k Cel3E hÚ˷gǗԺeҾKĕϧS‫ͱײ‬ΎʏgɋhȬdG]Z9rZ‫ײ‬Cel3C‫ײ‬Cel3D‫ײ‬Cel3F‫ײ‬Cel3G F
yk Cel1B h pNPG1 eȣU|ͱΎʏH G2 eͱՈS`‫מ‬K‫ײ‬Cel1A h G2 eȣU|ͱΎʏH‫מ‬G]Z9Ò˷‫ײ‬Ƙ BGL gÌ¯Ժ։ѱÒS‫ײ‬10%g G2 ǗԺbS`ѕՆЫΎʏԙ̺SZ9pNPG1 eҾ
KĕϧU| BGL ehՆЫϥʣϏHpbcЛԐQ}dG]ZH‫ײ‬G2 eҾKĕϧU| BGL (Cel1‫ײ‬Cel3A‫ײ‬
Cel3B Fyk Cel3E) eĥzGdѕՆЫϥʣϏHЛԐQ}Z9ՆЫϥʣϏeh‫ײ‬Ѭƙ͔ɩHϴd|͔:díѕwÊÇѕHƠr}‫ײ‬XgÜav¹ÊÐgƙʣ։h Cel1A b Cel3A HӘSK‫מ‬K‫̘ײ‬Ǳa
5.4 mg/ml b 2.8 mg/ml aB]Z9rZ‫ײ‬Cel3A ey|ՆЫϥʣϏḧ֚gѫթeĊ]`ÈÐeőӽ
Q}ΘǶSZH‫ײ‬Cel1A ahΤȬU|ObdKӚвQ}|ObĤzGbd]Z9OgObGz‫ײ‬Cel1A
HÈÆÐԒȪϏԺaB|¹ÊÐgƙʣe֛ØS`C|ƔҦʏHОƮQ}Z9
Enzymatic properties of β-glucosidase isozymes from Trichoderma reesei
Boyang Guo, Kouichi Nozaki, Masahiro Mizuno, Yoshihiko Amano(Dept. of Biosci. & Textile Technol., Interdisciplinary Graduate School of Sci. and Technol., Shinshu Univ.)- 66 —P-57¼£g³©ÈÆ©ÌÌÀ©ÇÐղĈȆg͓՟b͜Ҧgӽ̰ȖɅ‫ײ÷ך‬ŲϨȭ͚‫ײ‬Ϩ‫׮‬ӯí ‫֗װ‬Ȼ‫מ‬ȥÏϏԺɈ‫ױ‬؆ЉЃ؇¼£‫װ‬Tricholoma matsutake‫ױ‬eFC`‫ײ‬Xg‫{ח‬ϐʄ_M|‫ͳח‬ʣőaB|́Єփ¿¡Ègϥƙʣe֛U|ДӷhpbcdC9SGS‫®ײ‬Èah‫́ײ‬Єփ¿¡ÈH L-³©ÈÆ©Ì‫װ‬Phe‫ױ‬GźЄփѫ`ϥʣU|ObĤzGeQ}`C|9XgŔϿցѣbS` Phe y{́ЄփbÌÀ©
ϥʣU|ցѣaB| Phe ÌÀ©ÇÐ‫װ‬PAL‫ױ‬H͜ҦU|9XOa‫̤ײ‬Згah‫¼ײ‬£g PALղĈȆ‫װ‬TmPAL1‫ױ‬g cDNA Fyk¬¾ DNA ÊÐ©ÌS‫ײ‬ǱҰӑϿϝљey{¼£ PAL‫װ‬TmPAL1‫ױ‬ϦŐS‫ײ‬XgցѣŴȋЃʏԺgӽ̂ԊsZ9
؆˷·bѬ̲؇͋Ϗbցͯg PAL gģȉսœǗeԄԀSZ¥«ÉÐ¦µÆ ¼Ðb¼£‫װ‬NBRC30605 ̼‫ױ‬ӑјĒg¦ÐÈ RNA ϧCZ RT-PCR‫ײ‬3.Ɗk 5.RACE ·a TmPAL1 cDNA ǣɔS‫ײ‬XgŁ֗gǡǗսœͻȒSZ9TmPAL1 gˏȒ½¬փսœh‫ʹײ‬Ȇӑ¬ϩ̫g PAL b 60%
āÕgЋƛʏОSZ9TmPAL1 gÐ§‫ׇ‬Ǖh PCR‫ײ‬3.Ɗk 5.̣лgֿÐ§‫ׇ‬Ǖh Ì®Ð PCR
aǣɔSZɼ‫¾¬ײ‬gǡǗսœͻȒSZ‫׳‬cDNA b¬¾սœbgͱՈey{‫ײ‬7 ^g Ì¦ÊÌgȉǌЛԐSZ9rZ‫ײ‬4 ЮgŘ֣ցѣaŏϟSZ¬¾ DNA ϧCZÌӽ̰ey{‫ײ‬TmPAL1 hÌÈ²ÐaB|ObHОƮQ}Z9Qze‫ײ‬pET-28 ·ÐbǱҰӑ BL21(DE3)̼ϧC`‫ײ‬Ύʏ
̚U|Ѫ˔E TmPAL1 ϦŐSZ9ϝǌ‫ײ‬XgցѣŴȋЃʏԺԙo`C|9Structure and functional characterization of a phenylalanine ammonia-lyase gene, TmPAL1, in Tricholoma
matsutake.
Hayato Miyakawa, Naoki Katsuda, Yuji Tasaki(Dept. of Mater. Eng., Nagaoka Col. of Technol.)P-58‫ש‬ӑ hydrophobin RolA b cutinase CutL1 ֚gЋîĕϧe֛ØU| RolA gǡǗʏͩǗȩ‫ ט‬ӯԔ 1, Đӟ ǱԲ 1, ֊ Ĺƅ 1, ̨Ǔ Ńӆ 1, ÕƆ ĭí 1, ‫ ͛מ‬ʅ 2, ȹɳ Όɗ 3, ֡պ ˭ʕ 1
‫װ‬1 ̭ŵǱ֤ÏϥϏϦ͏ţʣ‫ײ‬2 ̭ŵǱÏ̢̫З‫ײ‬3 ̭õՎɈǱÏʊϧϥϏЧȋ‫ױ‬ hydrophobin hјϒӑFykʹȆӑgϐ̚dőΆÌ¯Ժa, ӑјӪ‫׀‬eȳǌU|9Aspergillus oryzae
gǞƙ, ϥőӽʏ»Ç¤ÈaB| polybutylene succinate-co-adipate(PBSA)ưÒgοѣΫbS`ǖ‫ה‬U|b hydrophobin RolA b PBSA őӽցѣaB| cutinase CutL1 ŅϿϝU|9PBSA eơАSZ RolA h
CutL1 bЋîĕϧS, PBSA Ӫ‫׀‬e CutL1 ι҅U|Oba PBSA őӽġբU|9O}ragЗгey]`, RolA b CutL1 gЋîĕϧeh ÌЃЋîĕϧH֛ØS, RolA Įg His32, Lys34 H CutL1 Įg Glu31,
Asp142, Asp171 HѬƙeևӵaB|ObHОQ}Z9SGS, RolA gֈϥǒ, H32S/K34S íևǫϴĒb
CutL1 gֈϥǒ, E31S/D142S/D171S ÔևǫϴĒϧC`ЋîĕϧgůťȋЃӽ̰ӧ]ZbO~, NaCl ȉǌ̈, RolA íևǫϴĒeȣU| CutL1 ÔևǫϴĒgѬƙ։H NaCl ֿȉǌ̈eͱՈS`‫׋‬ӘeΤȬSZ9OgѬ̲GzϐȒQ}ZͩǗāǭev ÌЃЋîĕϧպčHͩȉU|ƔҦʏHОƮQ}Z9XOa̤Згh, RolA íևǫϴĒeͩǌU| CutL1 bgЋîĕϧպčgӽ̂ƊkXgЋîĕϧ͓͜gӽ̰ЉЃbS
Z9ֹ͢Ӎ½¬փͩǗŴȋĥ‫ד‬SZ RolA íևǫϴĒbЋîĕϧe֛ØU|bˏΧQ}|ֹ͢Ӎ½¬փͩǗҋ˔SZ RolA ÔևǫϴĒĕӱS, QCM ey{ RolA-CutL1 ֚ЋîĕϧԉĝSZ9XgѬ̲,RolA Įg His32, Lys34 gýe Lys41, Lys46, Lys51 HЋîĕϧe֛ØS, His32, Lys34 bŅeǮĝŬ̲ey{ CutL1 bgЋîĕϧeȜØU|ObHОQ}Z9rZ, Kd ey{ "! "! 2"! g‫ׅ‬aЋîĕϧeȜØU|bˏΧQ}Z9
Aspergillus oryzae hydrophobin RolA possesses positive residues involving in RolA-cutinase Cutl1 interaction
Yusei Tsushima1, Daiki Sato1, Yoonkyung Kim1, Kimihide Muragaki1, Kenji Uehara1, Toru Takahashi2, Youhei
Yamagata3, Keietsu Abe1 (1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NRIB., 3 Tokyo univ. of Agric. And Technol)- 67 —P-59‫שק‬gӱ՟թЬeFM|Ё‫ש‬ӑg¦ÆÌÇµ¦Ð¾ӽ̰
íСΊǗ 1‫͉ײ‬Ò͚ 1, ƩϨ͢ǳչ 2, ͈Ɔɦƀ 2, ‫מ‬ÖФ̅ 2, Ǳ͉Ģչ 2, ϨĀɦú, 1 àƆƬ 1, ɼӟ͢ŗ 1
‫װ‬1 äǱ֤ÏՎ, 2 ÔƩտ‫ױ׌‬?ЉЃ@Ё‫ש‬ӑ‫װ‬Aspergillus kawachii‫ױ‬h‫ײ‬υվgօ՟eĘϧQ}|‫ש‬ӑaB|9̤Згah‫ײ‬Ё‫ש‬ӑgϐʄЃdʏԺgjb^aB|Ìփ‫מ‬ϥϦg¿©¾gŁĒķ̂zGeU|ObЉЃbS`‫קײ‬‫ש‬gӱ՟թЬeFM|Ё‫ש‬ӑg¦ÆÌÇµ¦Ð¾ӽ̰ӧ]Z9?˷·ÏҖȡ@rV‫קײ‬υվgƆ˳eĘϧQ}|Ǳ‫ײק‬Fykυվօ՟ϧgЁ‫ש‬ӑ‫͕װ‬ƒ̮áũƱɠ‫ױ‬
ϧC`ӱ‫ש‬SZ9ӱ‫ ש‬25 ֚̈Љe 401Gz 301nΦɢĎÖQWZ՝ɓ̪ă‫װ‬ÌփϥϦԒȪ̪ă‫ױ‬b‫ײ‬401ѸˀSZ̪ă‫װ‬ÌփϥϦֿԒȪ̪ă‫ױ‬Gz RNA ʸŐS‫ײ‬DNA ¼ ÊÉ ‫װ‬Agilent
Technologies‫ױ‬ey{ղĈȆϿϝ։ͱՈSZ9XgѬ̲‫ײ‬ÌփϥϦԒȪ̪ăeFC` 2 ĨāÕϿϝ։HǣŧSZ 296 ղĈȆ‫ײ‬FykΤȬSZ 250 ղĈȆƛȒSZ9O}zgղĈȆg Gene Ontology ӽ̰gѬ̲‫¬½ײ‬փ¦ÆÌ»ÐÐgϿϝǣŧbőȆÁ¸ÊÌgϿϝΤȬH‫ײ‬ÌփϥϦԒȪ̈gղĈȆgϿϝǫůgϐʄaB|ObHОƮQ}Z9rZ‫ײ‬401Gz 301eΦɢĎÖSZObey{‫מײ‬Φ¦ÉGzӽ֥Q}ZObey|bҖȡSZ9ϝǌ‫ײ‬ƛȒQ}ZϿϝǫůղĈȆgÜGzÌփg‫מ‬ϥϦe֛|bëΧQ}ZղĈȆg͜Ҧӽ̰բu`C|9Transcriptome analysis of white koji mold in a barley koji-making process
Taiki Futagami1, Kazuki Mori1, Shotaro Wada2, Yasuhiro Kajiwara 2, Hideharu Takashita2, Toshiro Omori2,
Kosuke Tashiro1, Satoru Kuhara1, Masatoshi Goto1(1Kyushu Univ., 2Sanwa Shurui Co., ltd.)P-60‫־‬ҋǖ‫ה‬љey|ЁҿҬ̥ʹȆӑ Phanerochaete chrysosporium gÆÌʣőʊу
e֛U|Зг
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gvgοѣΫbSZǖ‫ה‬љahƘʣőeȣU|ӑgʊуԙo|ObhֿɓeǁֶaB|9XgZu‫ײ‬O}rahѐ̣ÈÊÐǗԺbSZ˄bDǖ‫ה‬љHɜKϧCz}`IZH‫˄ײ‬bDǖ‫ה‬љhҷτϮeFM|̡̧gőӽϣǢGzGMֵ}`C|Zu‫ײ‬y{ҷτeՔC‫־‬ҋǖ‫ה‬љg͓ьHͶuz}`C|9̤Згah‫ײ‬ơʹʏÏģʹʏeĸ}ZÈÊÐ»ÌοѣΫbS`ϧC|Oba‫ʹײ‬Ȇӑ‫־‬ҋǖ‫ה‬љg͓ьԊsZ9rV‫ײ‬ÈÊÐ»Ìιɢ 2% w/v B|Ch 4% w/v a˄bDǖ‫ה‬b‫־‬ҋǖ‫ה‬ͱՈSZ9ÈÊÐ»Ìιɢ 2% w/v ahӑĒǭΙgÌ¯ԺιɢÏCel7 ΎʏŅe˄bDǖ‫ה‬g˷H‫מ‬CĪОSZH‫ײ‬ÈÊÐ»Ìιɢ 4% w/v ah՚ՆS‫־ײ‬ҋǖ‫ה‬gӑĒǭΙaCV}v‫מ‬CĪОSZ9͟e¼ ÊµÉÐ¦ϧC`ÈÊÐ»ÌbÆÌΞŧÈÊÐ»Ìǖ‫ה‬SZbO~‫ײ‬ÆÌgΞŧey]`ӑĒǭΙgÌ¯Ժιɢhћ 9 Ĩ‫ײ‬Cel7 Ύʏhћ 14 Ĩ‫מ‬Kd{‫ײ‬ÈÊÐőӽ֛ՠցѣgőΆHОƮQ}Z9ùɼhɲЗгȕaģ̚U|À¬Ì»Ð«Ì¦ցѣŏϟSZÆÌÈÊÐ»ÌeΞŧS`ǖ‫ה‬S‫ײ‬ƘʣőeȣU|ʹȆӑgʊу̂zGeS`CKëȒaB|9

Static cultivation of Phanerochaete chrysosporium to unveil responses to xylan components
Yuki Nakamura, Chiaki Hori, Kiyohiko Igarashi, Masahiro Samejima(Dept. Biomat. Sci., Univ. of Tokyo)- 68 —P-61‫ש‬ӑgÐºÌºÆ‫מ‬ϥϦ

¦ʰŘ֛ՠƿȆ CreAÏCreB íևИǦey|½ÆÐÒλ̈́Ȇ‫ײ‬ϨÜϡɌ‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå ‫̭װ‬ŵǱ֤ՎÏϥϏϦ͏ţʣ‫ױ‬‫ש‬ӑhǮ͔dǮѕ‫׌‬őӽցѣҎϥϦU|vgg8XgϿϝhÈÐey|ºÆ ¦ʰŘƐ
M|9јϒӑeFM|ÈÐʰŘhɜǕŘɿǒՆŋƿȆ CreA ey]`ŘɿQ}|ObHДz}`C|9À¥Èјϒӑ Aspergillus nidulans ah8CreA gÃ±¡ÌŴÏҪÃ±¡ÌŴey{ÈÐʰŘHŘɿQ}|ObHОƮQ}`F{8ÈÐȉǌÖah8ҪÃ±¡ÌŴցѣaB| CreB ey]` CreA HҪÃ±¡ÌŴQ}|bҖEz}`C|9XOa8̤Згah‫ש‬ӑeFC` creA, creB ſϔƊk creA/creB íևИǦ̼ĕӱS8O}zgղĈȆИǦgÈÐʰŘngɶ‫׃‬ӽ̰SZ9
creA Ɗk creA/creB íևИǦ̼ah̘ȫǖǎeFM|ϥҡgʖŴHӻȡQ}ZH8creB ИǦ̼ahϥ
ҡngɶ‫׃‬hӻȡQ}dG]Z9͟e8¥ÌµÌbÈÐΝƙSZȟǲǖǎa&-½ÆÐϥϦӻȡSZbO~8ֈϥ̼ah­ÊÐɳʣHӷz}dCgeȣS`8ƘИǦ̼aĥБd­ÊÐHӻȡQ
}Z9āÕgѬ̲Gz8ƘИǦ̼ahÈÐʰŘHӽ֥Q}`C|ObHОQ}Z9rZ8ÈÐΙĒǖǎeFC` creA Ɗk creA/creB íևИǦ̼ahֈϥ̼bͱՈS`&-½ÆÐΎʏgÕ̀H
Ԑuz}Z9QzeҺƦΛCObe8¥ÌµÌrZh¼È¦ÐοѣΫbU|ΙĒǖǎah creA/creBíևИǦ̼h creA ИǦ̼y{v‫׋‬Әe‫מ‬C&-½ÆÐΎʏОSZ9‫̤װ‬ЗгhϥЗÌÐ= ¬·ÐÄÌţŐǗНЃЗгˏբì͏>gˢ˖ƐM`ӧ}Z9‫ױ‬Hyperproduction of amylases by double deletion of CreA and CreB involved in carbon catabolite repressionin Aspergullus oryzae
Sakurako Ichinose, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.)P-62ÆÌԒȪǒ¿ÆՆŋƿȆ AmyR::XlnR ey|½ÆÐϥϦ֊Ȇĸɗ‫ ׶‬ϨÜȤǗ‫ ׷‬ÝðΜÒ̝‫ ׷‬ʉʹĺô‫ ׶‬ȫ̱ƬǴ‫ ׷‬ŧӟְǨ‫׶‬ ‫׶װ‬ƜǔǱ֤ÏՎ‫׷װױ‬ƜǱ֤ϥƨՎ‫ױ‬?ЉЃ@‫ש‬ӑah˺eɰťdÌ¯ԺϥϦљHЛиQ}`C|H8QzeIuѧGdԙъƔҦeU|
Zu8ՆŋƿȆѧőŴSŊ͓ьU|Oba=ՆŋƿȆɈȋ>bvCDoI8˶ZdՆŋŘɿʮӨg֙ϿӧDObЉЃbSZ9ùƾhXgпÒͤbS`8ÆÌőӽցѣgՆŋƿȆ XlnR b½ÆÐղĈȆҎgՆŋƿȆ AmyR bg¿ÆՆŋƿȆĕӱS8ӽ̰ӧ]Z9?˷·bѬ̲@N ̣лeh
FLAG-His-tag þŧS8AmyR g DNA Ѭƙ§¿ÌƠt N ̣лĮ 68aa b XlnR g DNA Ѭƙ§¿

Ì

ЊɼGz C ̣лrag 812aa ՠѬS8AmyR::XlnR ¿ÆՆŋƿȆ͓ьSZ9O} amyR ͞Ƕ̼eFC`ϿϝQW8ʲ FLAG ʲĒϧCZÌ´Ê¢¤Ìey{8AmyR::XlnR ¿ÆՆŋƿȆgϿϝЛԐSZ9rZ8AmyR::XlnR ¿ÆՆŋƿȆϿϝ̼h CMC w¥ÌµÌgΞŧah½ÆÐΎʏhpbc͌ŐQ}dCgeȣS8ÆÌƊkÊÐΞŧSZǞƙh8‫׋‬Әd½ÆÐΎ
ʏH͌ŐQ}Z9
A model chimeric transcription factor: construction and characterization of an AmyR::XlnR hybridtranscription factor.
Yuhei KANEKO1, Hisaki TANAKA2, Jun-ichiro MARUI‫׷‬, Motoyuki SHIMIZU1, Tetsuo KOBAYASHI2, Masashi
KATO1.

1

Meijo Univ., 2 Nagoya Univ.

- 69 —P-63 (O-6)
Trichoderma reesei eFM|ÈÆÐϥϦe֛ØU|¦ÆÌ»ÐÐgӽ̰ԦƒǱ͚‫˻ײ‬ÖФӧ‫ײ‬ƓɅ֩њ‫ײ‬ʉϨΌú‫ײ‬ȫоƆΡ ‫֗װ‬ȻʮЧǱÏϥϏ‫ױ‬јϒӑ Trichoderma reesei h8ѧҥǭÈÊÐсgԒȪϏԺgȉǌÖaÈÆÐԒȪЃeϥϦU|9SGS8×έʏgÈÊÐhѧҥҲ՛թU|ObHŐ̫dCZu8cgyDeS`O}ԐԢS8
Xg¨ÈӑĒŉeĈիS`C|gGh×̂aB|9O}ragЗгGz͓ʣЃeϿϝS`C|ÈÆÐH×έʏgÈÊÐőӽS8OgőӽϦϏHƏ{Ցr}|Obey]`ÈÆÐHԒȪϥϦQ}|bҖEz}`C|H8XgԎѧe֛S`v×̂aB|9O}raɲЗгȕahÈÆÐbƛԙЃeϿϝS8G^ŔϿgÈÆÐԒȪϏԺgƏ{Ցse֛|bˏȒQ}|¦ÆÌ»ÐÐe֛S`ÈÆÐ‫מ‬ϥϦӑ̼aB| PC-3-7 aӽ̰S`IZ9Xgӽ̰gթЬaϿӷQ}Z MFS ¦ÆÌ»ÐÐ
TRP3405 h8PC-3-7 ̼eFC`ղĈȆИǦӧ]ZbO~8ИǦ̼eFC`±È8ÈÊÐ8Æ¦ÐeFM|ÈÆÐϥϦHǶ}`C|ObĤzGbd]Z9XOa8TRP3405 ղĈȆgИǦH8ÈÆÐϥϦe֛|ēzGgևӵdղĈȆgϿϝeɶ‫׃‬ØE|bҖE8PC%3405 ̼b PC-3-7 ̼eFM|Ê±ÐԒȪԊ‫כ‬g¼ ÊÉ ӽ̰ӧ]`C|9XgѬ̲Gz PC%3405 ̼eFC`ÞӵdÈÆÐÏ¶½ÈÆÐgϿϝHǶ}`C|ObHŕ̂SZ9ϝǌh8PC%3405 ̼b PC-3-7
̼֚eFM|ՆŋԙъղĈȆҎw¦ÆÌ»ÐÐղĈȆҎсe֛U|Ԏѧdӽ̰ӧ]`C|
Influence of gene destruction of cellulose specific transporter in Trichoderma reesei
Hiroki Taniguchi, Hideyuki Kusaka, Tkanori Furukawa, Yosuke Shida, Wataru Ogasawara(Nagaoka Univ. of Tech)

P-64
Aspergillus nidulans ՆŋƿȆ ManS g͜ҦΥնñåȆ‫ּײ‬ȹ̢̫‫֊ײ‬ÝõȆ‫ ̨̡ײ‬Ў‫ײ‬ȫ̱ƬǴ ‫װ‬ƜǱ֤ϥƨՎÏϥϏ͓͜‫ױ‬?ЉЃ@ A. oryzae eFC`ƛȒQ}ZՆŋƿȆ ManR h‫¼ײ‬Ì¨¨ÐÏÈÆÐϥϦeʇ‫׆‬gƿ
ȆaB|9Ò˷‫ײ‬A. nidulans ah manR eŧE`Xg¹ÀÊaB| manS ̚S`F{‫ ײ‬manR b manS
gſϔИǦ̼ah¼Ì¨¨ÐϥϦҦgȏŁdΘǶehҸzdCH‫ײ‬íևИǦ̼ahȏŁe¼Ì¨¨ÐϥϦʏHǶ}|9̤Згah manS ŘɿÖg® ¼őӽցѣղĈȆgѺҌЃƛȒӧ]Z9?˷·ÏѬ̲@ֈϥ̼, %manS ̼ konjac glucomannan (KG)ſÒοѣΫbS` 24 ֚̈ԒȪS, ʸŐSZ RNA ϧC` RNA ÐÌÌӧ]ZѬ̲‫ײ‬Ì§¼Ì¨¨ÐղĈȆaB| manC, manE,
manF ղĈȆH ManS ey|ŘɿƐM`C|ObHОQ}Z9ManR ŘɿgղĈȆh manB b manC aB{‫ײ‬manC ֥Kb ManR b ManS hϴd|¼Ì¨¨ÐղĈȆŘɿS`C|ObHОQ}Z9ManR
b ManS ey{Ņ՝S`ŘɿQ}|ǮѕőӽցѣղĈȆh manC [MadK‫ײ‬eglA‫ײ‬eglB‫ײ‬cbhA‫ײ‬cbhD‫ײ‬
faeC H˂Nz}Z9Ud\‫ײ‬ManR ˢսgÒպgÞӵÈÆÐղĈȆh ManS ey]`vŘɿQ}`
CZ9CMC ey|ԒȪah manS ИǦH EglA‫ײ‬EglB ϥϦeɶ‫׃‬ØEdCObҖʠU|b‫ײ‬ManS hϴd|ԒȪϏԺeʊуS` ManR bÒպևӲU|ղĈȆŘɿU|bҖEz}|9ϝǌ‫ײ‬ÇÌփҳγÈÊÐ‫ײ‬KG ԒȪϏԺbS` manS ИǦHˏȒ¼Ì¨¨Ð‫ײ‬ÈÆÐղĈȆҎgϿϝeØE|ɶ‫׃‬e^C` Northern ´Ê¢¦ӽ̰ey{ЛԐӧ]`C|9̤Згh‫ײ‬ϥЗÌÐg ¬·ÐÄÌţŐǗНЃЗгˏբì͏gÒϣbS`ӧ}ZvgaB|9
Physiological function of ManS, a fungal-specific transcription factor in Aspergillus nidulans
Ayako Watanabe, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ. )- 70 —P-65°ÆgÇ©ÌőӽցѣϿϝԙъ͓͜eFM|ÈÀ¥ÂÇÌgɷŢ̣ō‫מ‬ʉ 1‫ץ̤֞ײ‬ӧ 1‫ײ‬ʃ͵ТȊ 1‫̤ײ‬ϨØÒ 2‫΅ײ‬΍ɬđ 1‫̡֋ײ‬ÒȔ 1‫ײ‬ŀ͹ĢÒ 1(1 ίԴЍǱÏϣ‫ײ‬2 õǱÏՎ) āŜgӽ̰y{‫ײ‬ĀӪЃd Ca2+ʗǝĈիÌ¯ԺgÒ^aB|ÈÀ¥ÂÇÌ‫װ‬CaM‫ױ‬HЁҿҬ̥ӑ Phanerochaete chrysosporium eFM|ÞӵÇ©ÌőӽցѣϿϝ͢eԙъU|ObHОƮQ}`
C|9Pleurotus ostreatus‫װ‬°Æ‫ױ‬hÞӵÇ©ÌőӽցѣbS`Æ¢Ð‫װ‬Lac‫ױ‬Fyk¼ÌÌ¸È

Ð‫װ‬MnP‫ױ‬ϥϦU|ǅŉҷϥgЁҿҬ̥ӑaB|9°Æ PC9 ̼ϧC`‫ײ‬CaMթšϿϝ̼b shRNA Ͽϝey|Ͽϝʰ̼ĕʣSZbO~‫ײ‬Ŝҗh Lac Fyk MnP ΎʏHΤȬS‫ײ‬ɼҗhǣŧSZ9rZ‫ײ‬ֈϥǒ̼e CaM ֠ȘŞ W-7 ΞŧSZǞƙv Lac Fyk MnP ΎʏHǣŧU|ObHӻȡQ}Z9āÕgObGz‫ײ‬°ÆeFC` CaM hÇ©ÌőӽցѣϿϝԪeԙъU|Ob
HОƮQ}Z9ϝǌ‫ײ‬ԎѧdŘɿ͓͜ӽ̰U|Zu‫ײ‬Lac Fyk MnP Þӵ¾ղĈȆgՆŋϏ։ӽ̰ӧ]`C|9

Role of calmodulin in regulation of ligninolytic enzymes expression in Pleurotus ostreatus.
Takashi Suetomi1, Takaiku Sakamoto1, Yoshitaka Tokunaga1, Yoichi Honda2, Kousuke Izumitsu1, Kazumi Suzuki1
and Toshikazu Irie1(1Unvi. of Shiga Pref., 2Kyoto Univ.)

P-66
Aspergillus aculeatus ÈÊÐљ®
Ȓ

¼őӽցѣղĈȆgϿϝŘɿƿȆgƛզéҒǳ‫ײ‬ǆͣѲҍ‫ײ‬Ԧĥ΂‫ײ‬οԦ‫ׅ‬Ò‫ײ‬ɅƒŝƗ‫֞װ‬ɡǱ֤ÏϥϣЧ‫ױ‬јϒӑ Aspergillus aculeatus eFM|ÈÆÐϥϦԙъ͓͜őȆÉ·Èaӽ̂U|Zu‫ײ‬ʤ:h˶ZdÈÆÐϥϦԙъƿȆƛȒS‫ײ‬Xgĕϧ͜ɟӽ̂U|ObЉˁS`C|9cellobiohydrolase I ղ Ĉ Ȇ (cbhI) µ Ê À Ð  ˢ ս Ö a É » Ð  b S ` orotidine 5’-phosphate
decarboxylase ղĈȆϿϝU|yDeĕӱSZ̼țÞeϧC` T-DNA ˅ŀǫϴ̼Æ´ÆÇ͓ьS‫ײ‬XgÜGz 5-fluoroorotic acid (5-FOA) ƠtÈÊÐǖǎÕaϥҡSZ̼ձʴSZ9ɾz}Z 5-FOA
Ҙʏ̼gÜGz‫ײ‬ÈÊÐԵŴҦHĎÖSZ̼ŘɿƿȆ͞˗ĩӰ̼bS`ſֵSZ9ƘĩӰ̼eFM| cbhI Ͽϝ։¬ÐÌ´Ê¢¦·ey{Ȓ։SZѬ̲‫ײ‬ԒȪ̪ăÖeFC`v cbhI Ͽϝ։H͌Ő֣ϮāÖraΤȬS`CZĩӰ̼ (B7 ̼) ƏɾSZ9inverse PCR ·ϧC` B7 ̼eFM| T-DNA ˅ŀɣč֣ȒS‫ײ‬XgƥՏgղĈȆ pPTRII µÆ½§ϧC` B7 ̼eȪŀSZbO~‫ײ‬B|ղĈȆ(gene1) gȪŀey{ cbhI Ͽϝ։‫ײ‬ÈÊÐԵŴҦHŅeÌ¦ÊÐÈ̼É·ÈraƾʀSZ9ϝǌh
gene1 gſϔ͞˗̼FykXgЋӰ̼ĕŐS‫ײ‬ÈÊÐљ®

¼őӽցѣϥϦ։FykƘղĈȆϿϝ։ӽ̰S`C|9
Identification of regulator(s) controlling the expression of cellulase genes in Aspergillus aculeatus
Syota Yuki, Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi(Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.)- 71 —P-67
Expression cluster analysis of the transcriptomes at sequential growth stages in thewhite-rot fungus, Phanerochaete carnosa, grown on aspen and spruce woods.
Hitoshi Suzuki (RIKEN, CSRS), Philip Wong, Chi-Yip Ho, Yunchen Gong, Kin Chan, Elisabeth Tillier andEmma Master (Univ. of Toronto)
A white-rot fungus, Phanerochaete carnosa, is a natural decomposer of coniferous wood and has an ability todegrade various types of softwood and hardwood tissues. While proteins produced by P. carnosa are expected tocomprise promising enzymes for bioprocessing lignocellulose including fibre from coniferous wood, genome
sequencing revealed that a large contingent of likely relevant proteins remain uncharacterized.
Here, we performed gene expression cluster analysis using multiple P. carnosa transcriptomes obtained at
different stages of fungal growth on trembling aspen and white spruce. The results revealed co-expression pairs
and clusters of known lignocellulolytic genes associated with genes of unknown functions (UNKs), suggesting
participation of those unknowns in the lignocellulolytic system of this fungus.

P-68‫ש‬ӑgՆŋƿȆ AmyR b MalR gғԆɼĥ‫ד‬bȎȒʏ֋̡дǳ‫ײ‬ϨÜϡɌ‫˶ײ‬Ԧȭɬ‫ײ‬ïƦŲå ‫̭װ‬ŵǱ֤ÏՎÏϥϏϦ͏ţʣ‫ױ‬‫ש‬ӑg½ÆÐϥϦeh‫½ײ‬ÆÐղĈȆҎЊˎŘɿU| AmyR b¼È¦ÐƏ{ՑsљŘɿU| MalR g 2 ЮgՆŋƿȆH֛ØS`C|9O}zgՆŋƿȆhԒȪǗԺg̚ςe֛zV͓ʣЃeϿϝS`C|Zu‫ײ‬ԒȪǗԺĜȉЃeғԆɼĥ‫ד‬ƐM‫ײ‬Ì¯ԺÉ·ÈaΎʏŴU|bëʙQ}`C|9̆ɘɢg̤ćeFC`‫ײ‬ʤ:h AmyR b MalR Hϴd|ѧҥŉȳǌОUObǝƤSZ‫װ‬1‫ױ‬9̤Згah‫ײ‬Ì¯ԺӥƙQWZ AmyR b MalR ‫ש‬ӑaϿϝQW‫ײ‬Ì´Ê¢¦ӽ̰ey{
AmyR b MalR gƘЮοѣΫeFM|Ì¯Ժĥ‫ד‬wȎȒʏe^C`ӽ̰SZ9
 ¡½ÌĜȉЃeՆŋHʰŘQ}| thiA µÊÀÐÐŘɿÖa8Ì¯ԺþŧSZ AmyR b
MalR gϿϝ̼ĕӱSZ9O}zϿϝ̼½¬փοѣΫbSZǖǎaŜǖ‫ה‬SZɼ‫ײ‬ԒȪǗԺb¡½ÌΞŧS‫ײ‬ѫ̈ЃeӑĒƾƎSZ9ӑĒGzÌ¯ԺʸŐS‫ײ‬Ì´Ê¢¦ӽ̰eěSZbO~‫ײ‬AmyR hԒȪǗԺĜȉЃe՞wGe®Ì§HΘǶSZgeȣS8MalR hȎȒaB]Z9
OgObGz‫ײ‬MalR bϴd{‫ײ‬AmyR hԒȪǗԺĜȉЃe՞wGdőӽƐM|ƔҦʏHОƮQ}Z9rZ‫ײ‬AmyR h phosphatase ŏϟey{¨ÈHĎőȆ։Įe³¦SZObGz‫ײ‬ÇÌփŴĥ‫ד‬ƐM`C|ObHОQ}Z9ùɼh Phos-tag SDS-PAGE ϧC`‫ײ‬O}zՆŋƿȆgÇÌփŴÉ·Èӽ̰
U|ëȒaB|9‫̤װ‬ЗгhϥЗÌÐ= ¬·ÐÄÌţŐǗНЃЗгˏբì͏>gˢ˖ƐM`ӧ}Z9‫ױ‬‫װ‬1‫̡֋ױ‬дǳpG‫׿‬п 12 ƾјϒӑőȆϥϏȋÌ³ÉÌӵ˼ֱ‫ײ‬p.57
Post-translational modification and stability of transcription factors, AmyR and MalR, in Aspergillus oryzae.
Kuta Suzuki, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ)- 72 —P-69
pH ʊуՆŋƿȆ PacC ey|јϒӑÈÆÐղĈȆϿϝŘɿǆͣѲҍ 1‫ײ‬ӕƆǱР 2‫ײ‬Ṳ̑ĭǳչ 1‫֊ײ‬ÝõȆ 1‫̨̡ײ‬Ў 1‫ײ‬ȫ̱ƬǴ 1‫װ‬1 ƜǱ֤ϥƨՎÏϥϏ͓͜‫ײ‬2 źӗǱÏЎӑÌÐ‫ױ‬?ЉЃ@Aspergillus nidulans eFC`‫ײ‬pH ¨ÈĈիƿȆ PalC ղĈȆgǫϴey]`ÈÆÐϥϦ
ʏHӘSKĎÖU|ObӷŐS`C|9XOa‫ײ‬PalC gÖΏeȉǌU| C2H2 ǒՆŋƿȆ PacC gղĈȆИǦ̼ĕӱSZbO~‫ײ‬PalC ǫϴ̼bƛ͔eÈÆÐϥϦʏgĎÖHӻȡQ}Z9̤Згah PacCg֛Øy{Ԏѧeԙo|Zu‫ײ‬pacC ИǦ̼eFM|ϣǢ pH eʊуSZÈÆÐղĈȆҎgϿϝӽ̰ӧ]Z9?Ѭ̲@փʏ (pH 4.2) ƊkÈÇ (pH 8.2) Ú̪ăÖeFM|Ê±ÐԒȪ̈gƘÈÆÐղĈȆgՆŋӽ̰ qRT-PCR ·ϧC`ӧ]Z9ֈϥ̼eFC`‫ײ‬փʏ̪ăÖahԒȪɼ‫֚̈׸‬aՆŋg²Ð
ՓE|Ò˷a‫ײ‬ÈÇ̪ăÖah‫֚̈׶‬ɼb՞wGdՆŋHӻȡQ}Z9ΔpacC ̼ahCV}g̪ăÖavֈϥ̼pcgՆŋ։gÕ̀hӷz}dG]Z9É»ÐÐ¢ ey{ eglA µÊÀÐgǫϴӽ̰ӧ]ZѬ̲‫ײ‬PacC Ѭƙսœ͞ǶƉhǫϴQW`v pH ʊуʏHӷz}ZZu‫ײ‬PacC ey| eglAgŘɿh֚ˎЃaB|ObĤzGed]Z9ÈÆÐղĈȆgϿϝŘɿƿȆղĈȆg qRT-PCR ӽ̰Ѭ̲Gz‫ײ‬O}zgԙъúSZ֚ˎŘɿgƔҦʏhƟȒQ}Z9XOa‫ײ‬PacC ŘɿÖeB|ղĈȆe^C`ѺҌЃeԙo|Zu‫ײ‬ÈÇ̪ăgÌµÈ RNA-seq eěSZbO~‫ײ‬ManR ĜȉЃeԙъQ}|ղĈȆgՆŋHΔpacC ̼aΤȬS‫ײ‬rZӲˮgˏȒѕՍՙĒղĈȆH PacC ŘɿÖeB|ObHŕ̂SZ9TLC ӽ̰ey{ΔpacC ̼ahֈϥ̼bͱՈS`ǖ‫ה‬ÕΟÜeÊ±ÐHǮKͩȉS`CZOb
Gz‫ײ‬PacC hÊ±ÐgƏ{ՑsúS`‫ײ‬ManR gΎʏŴŘɿS`C|bҖEz}|9O}zgѕՍՙĒղĈȆИǦ̼ĕӱS‫ײ‬pH eʊуSZÊ±ÐgƏ{ՑsƊkÈÆÐϥϦʏe^C`
ӽ̰U|ëȒaB|9̤Згh‫ײ‬ϥЗÌÐg ¬·ÐÄÌţŐǗНЃЗгˏբì͏gÒϣbS`ӧ}ZvgaB|9
The pH-signaling transcription factor PacC regulates expression of cellulase genes in Aspergillus nidulans.
Emi Kunitake1, Daisuke Hagiwara2, Kentaro Miyamoto1, Kyoko Kanamaru1, Makoto Kimura1, Tetsuo Kobayashi1(1Graduate Sch. of Bioagric. Sci., Nagoya Univ., 2MMRC, Chiba Univ.)P-70‫ר‬ӑ xyrA Ɗk larA ղĈȆg XlnR b AraR ey|ŽԙЃϿϝŘɿЖɅ‫ח‬ž‫ײ‬Ʌλ̏ҍ‫ײ‬ֈƒđƗ‫֊ײ‬ÝõȆ‫̨̡ײ‬Ў‫ײ‬ȫ̱ƬǴ ‫װ‬ƜǱ֤ϥƨՎÏϥϏ͓͜‫ױ‬?ЉЃ@ XlnR b AraR h Aspergillus ȷјϒӑgv^ Zn(II)2Cys6 ǒgՆŋƿȆaB|9O}zƿȆhCV}v¸Ì¦ÐĀԠљղĈȆҎ͢eŘɿU|H‫ײ‬ղĈȆey]`ĥzGdÚҗgмƙHӷz}|9OgŽԙЃG^ÒպмƙЃdŘɿ͓͜gԎѧ̂zGeU|Zu‫̤ײ‬Згah‫¸ײ‬Ì¦ÐĀԠљgпÒ֪ͫgցѣÐ§U| xyrA Ɗk larA gµÊÀÐÐ‫ׇ‬ǕeȉǌU| XlnR‫ײ‬AraR ѬƙպčƛȒSZ9XgѬ̲vbe‫ײ‬ŽԙЃG^мƙЃdŘɿ͓͜e^C`ҖȡU|9?˷·ÏѬ̲@ǱҰӑțÞbS`ϥϦSZ XlnR‫ײ‬AraR g DNA Ѭƙ§¿ ÌϧC‫ײ‬xyrA‫ײ‬larA µÊÀÐÐeȉǌU| DNA Ѭƙպč EMSA ey{ƛȒSZ9xyrA µÊÀÐÐah‫ײ‬XlnR h 2 ʩg
5’-GGCTAA-3’‫ײ‬AraR h 5’-CGGGTAAA-3’eѬƙSZ9O}zh˺ДgѬƙÌÌսœaB|9Ѭƙսœh 60 bp g‫ׇ‬ǕŉeȉǌS8̘Еg XlnR‫ײ‬AraR Ѭƙսœ֚gՁֵh 11 bp aB]Z9larA µÊÀÐ
Ðah8AraR h 3 ʩgÌÌսœ‫ײ‬XlnR h 5’-GGATAA-3’bCD˶ӸսœeѬƙU|ObH
ОQ}81 ʩg AraR Ѭƙսœ֥KbO}zѬƙսœh 40bp ŉeֱвS`F{8ŉ‫׶‬ʩah XlnRb AraR ѬƙսœhևӲS`C|ObHОƮQ}|9āÕGz‫ײ‬XlnR8AraR hÒպgѬƙսœeFC`мƙU|bҖEz}‫ײ‬XgԈʾvɾz}^^B|9ɽ]`‫ײ‬XlnR‫ײ‬AraR g̽ŉgÀÈͱ‫ײ‬X}Y}gΎʏŴϒʝ‫ײ‬мƙѬƙ‫ׇ‬ǕgϿϝÉ·ÈngԬϖɢH xyrA‫ײ‬larA g̘ѩЃϿϝÉ·ÈͻȒU|bҖEz}|9 ̤Згh8ϥЗÌÐg ¬·ÐÄÌţŐǗНЃЗгˏբì͏gÒϣbS`ӧ}ZvgaB|9Co-regulation of A. oryzae xyrA and larA expression by XlnR and AraR
Kana Ishikawa, Tomomi Kawase, Yuji Noguchi, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci, Nagoya Univ)- 73 —P-71
Aspergillus aculeatus Ê±ÐʊуƿȆ ClbR bXg¯ÆÊƿȆ ClbR2 gĕϧ͜ɟӽ̰
Ʌ̨ɵß‫ײ‬ǆͣѲҍ‫ײ‬Ԧĥ΂‫ײ‬οԦ‫ׅ‬Ò‫ײ‬ɅƒŝƗ‫֞װ‬ɡǱ֤ÏϥϣЧ‫ױ‬јϒӑ Aspergillus aculeatus eFC`ÈÊÐey|ղĈȆϿϝԒȪ͢eŘɿU|ƿȆ cellobiose
response regulator (ClbR) h‫ײ‬ÈÊÐeʊуS` XlnR ĜȉЃÏֿĜȉЃeԒȪQ}|ղĈȆgϿϝòբU|9ClbR ͓ʣЃe‫מ‬ϿϝU|̼ĕӱS‫ײ‬ȫ‫ק‬lUrοѣΫbS`ǖ‫ה‬SZbO~‫ײ‬Xgǖ‫ה‬ÕΟÜeh ClbR ŘɿÖgղĈȆϦϏgD\ XynIa gőΆ։HŤЃeǣŧS`F{‫ײ‬rZXgՆŋ։vƛ͔eǣŧS`CZH‫ײ‬XgýgղĈȆgϿϝhòբQ}dG]Z9rZ Yeast Two Hybrid (Y2H) ·ey{ ClbR gЋîĕϧƿȆˍѤSZbO~‫ײ‬ClbR bЋƛʏ 42%g¯ÆÊƿȆ ClbR2 g C ̣л͞˗Ì¯Ժ(ClbR21-295) HƛȒQ}Z9clbR2 ſϔИǦ̼b clbR bgíևИǦ̼ĕӱS‫ײ‬Ƙ̼eFM|ղĈȆϿϝ։Ȓ։SZѬ̲‫ײ‬ClbR2 h ClbR bŽԙЃeՆŋƿȆ ManR gϿϝŘɿU|Ò˷a‫ײ‬
ClbR ŘɿÖeB| FIa-xylanase (xynIa), FIb-xylanase (xynIb) ղĈȆgϿϝeh֛ØS`CdCObHОƮQ}Z9
XOa ClbR g in vitro eFM| DNA ngѬƙϐʏӽ̰U|Zue‫ײ‬ClbR Ł֗ (MalE-ClbRwhole)b xynIa µÊÀÐÐ‫ׇ‬ǕϧC` Electrophoresis mobility shift assay ӧ]ZѬ̲‫ײ‬³¦®Ì§H͌ŐQ } Z 9 ϝ ǌ ‫ ײ‬Y2H e y { ClbR b g Ћ î ĕ ϧ H О Q } Z ClbR2 g C ̣ л ͞ ˗  Ì ¯  Ժ(MalE-ClbR21-295) ϧC`‫ײ‬ClbR Fyk ClbR2 g xynIa Fyk manR µÊÀÐÐ‫ׇ‬ǕeȣU|Ѭƙϐʏӽ̰S`C|9Functional analysis of cellobiose response regulator (ClbR) and its paralog (ClbR2) in Aspergillus aculeatus.
Ayano Kawamura, Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi(Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.)

P-72
Molecular basis that divides ManR-dependent and ManR/McmA-dependent genes inAspergillus nidulans
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Grad. Sch. Bioagric. Sci., Nagoya Univ.)
Fungal hydrolytic gene expression is tightly controlled at the transcription level. Besides the traditional
XlnR-dependent mechanism, a novel McmA-ManR mechanism involved in regulation of cellulolytic andhemicellulolytic genes has been identified recently. Both transcription factors are essential to inductive expressionof eglA, encoding endoglucanase A, because ManR requires McmA for binding to CeRE (Cellulose Responsive
Element) on the promoter. Based on RNA sequencing analysis, highly expressed cellulolytic genes are under control
by both McmA and ManR (ManR/McmA dependent). But at the same time a number of genes are ManR-dependent
but not McmA-dependent (ManR-dependent). The ManR-dependent genes include some mannanolytic andxylanolytic genes. In this report, we confirmed the requirement of McmA for ManR-binding using the promoter of
eglB, another endoglucanse gene, and also examined whether ManR without McmA could bind the promoter of the
ManR-dependent gene mndB encoding β-mannosidase.
Binding of His-tagged McmA and Flag-tagged ManR DNA binding domain to various probes were examined by
EMSA. When binding to the eglB was examined results similar to eglA were obtained. ManR did not bind to thepromoter in the absence of McmA, while it bound to CeRE containing DNA fragments in its presence. McmA alone
could bind to various locations on the promoter, but CeRE was required for recruitment of ManR. This implies
ManR not only binds to McmA but also to DNA. In contrast, ManR could bind to the mndB promoter in the absenceof McmA. Since no CeRE-like sequence exists on the promoter, it appeared that ManR recognized a sequence
different from CeRE. Further study will focus on the identification of the binding site for ManR on the mndB
promoter.
This work was supported by the Programme for Promotion of Basic and Applied Research for Innovation in
Bio-oriented Industry.

- 74 —P-73јϒӑ Aspergillus niger ϩ̫ PKS-NRPS ղĈȆÆÐgɰŘϿϝΚɅȊҏ‫͍ײ‬ȫ8Ҩ̤˨ə8֡պո̝ ‫̭װ‬Ǳ֤Ӟ‫ױ‬јϒӑ Aspergillus niger h‫^׽‬g polyketide synthase – non-ribosomal peptide synthase (PKS-NRPS)ղĈȆ
̚U|H‫ײ‬XgŁ`gϥʣϏh̢ƛȒaB|9y]`‫ײ‬O}z̢ДgϥʣϏƛȒU|Oba‫˶ײ‬ӸdϥƙʣgДӷHɾz}|ObH̠ɺQ}|9̤Згah‫ײ‬XgD\gÒ^g PKS-NRPS ղĈȆ An11g00250(pynA)ƠtղĈȆÆÐ(pyn ÆÐbƨƜ)eΉЉSZ9Pyn ÆÐh͢gՆŋƿȆaB|pynR ղĈȆƠt9PynR h GAL4 ‫׌‬ČgՆŋƿȆaB{‫ײ‬ղĈȆÆÐeƠr}|ղĈȆŁ`gՆŋԒȪU|bҖEz}Z9XgZu‫ײ‬A. niger ATCC 1015 ̼e PEG-µÊ¦µÆ¦·e` pynR ÕΏe
gpdA promoter þŧSZvgȪŀS‫ײ‬ÈÐƠtǖǎaɳԺՆ˔Ēǖ‫ה‬S` pynR ɰŘϿϝ
U|Oba‫ײ‬pyn ÆÐgϥʣϏg͌ŐԊsZ9SGS‫ײ‬Ѭ̲bS`ϥʣϏhɾz}V‫ײ‬RT-PCRgѬ̲‫ײ‬ÈÐȉǌÖah‫ײ‬pynR hՆŋQ}|vgg‫ײ‬pynA hՆŋQ}dCObHŕ̂SZ9XO
a8pyn ÆÐhÈÐȉǌÖ‫ײ‬ՆŋHʰŘQ}`C|gahdCGbëʙS‫ײ‬ÈÐֿĜȉǒg aga µÊÀÐÐ pynR ÕΏe˅ŀSZɳԺՆ˔ĒĕӱSZ9XgѬ̲‫ײ‬ÈÐƠrdCԒȪǖǎa8ɳԺՆ˔Ēǖ‫ה‬U|Oba‫ײ‬Ì¦ÊÐÈehdC‫˶ײ‬ZdŴƙϏHϥϦQ}|ObH
ЛԐQ}Z9OgŴƙϏǱ։ԙӱS‫ײ‬NMR‫ײ‬MS ő̰eěU|Oba‫˶ײ‬ӸŴƙϏaB| pyranonigrin E
aB|bƛȒSZ9XgϥƙʣѫՂe^C`vϿӪe`ҖȡU|9Expression of the dormant PKS-NRPS gene cluster in Aspergillus niger
Takayoshi Awakawa, Xiao-Long Yang, Toshiyuki Wakimoto, Ikuro Abe(Grad. School of Pharm. Sci., Univ. of Tokyo)

P-74¦Ç¤ÌϥƙʣղĈȆgՆŋƿȆÐ§U| Tri6 g͜Ҧӽ̰ÜɄ đÒ‫ ׈נײ‬Ҿə‫ײ‬ŜϨ Òӧ‫ײ‬ɏɅ ִĀ‫ײ‬ȫ̱ ƬǴ‫ ̨̡ײ‬Ў ‫װ‬ƜǱ֤ÏϥƨՎ‫ױ‬Fusarium graminearum h¦Ç¤ÌљͰѣϦϥS‫ײ‬țÞ͋Ϗnʜ̸U|֬gϺƆƿȆbS`ϧC|9ͰѣϦϥ̪ăÖah‫¦ײ‬Ç¤ÌϥƙʣղĈȆ‫װ‬Tri ղĈȆ‫ױ‬ÆÐŉeȉǌU| Tri6 ղĈȆHÐ§U|ՆŋƿȆb Tri10 ղĈȆHÐ§U|͜Ҧ̢ДgŘɿÌ¯ԺgĶIey{ѫՂցѣղĈȆ Tri5
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ղĈȆbýgѫՂ Tri ղĈȆgՆŋΎʏŴhϴd| TRI6 ӲƙĒgɳʣúS`C|ObHОƮQ}Z9 ̤ЗгhϥЗÌÐ ¬·ÐÄÌţŐì͏gˢ˖ƐM`ӧ]Z9Functional analysis of Tri6 that encodes a transcriptional factor for trichothecene biosynthesis.
Yuichi Nakajima, Yoshiyuki Kito, Kazuyuki Maeda, Hinayo Ichikawa, Tetsuo Kobayashi, Makoto Kimura(Graduate Sch. of Bioagric. Sci., Nagoya Univ.)- 75 —P-75
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Production control mechanism of terpendole mycotoxins
Takayuki Motoyama, Hiroyuki Osada(Antibiotics Lab., RIKEN)

P-76«Cv\ϺӑeFM|¤ªÌփgϥϦŘɿ¿©¾ƊkϥϟΎʏȯʋ֍‫̤ײ‬ȹ‫מ‬ə‫ײ‬į́Ƌƕ8֗Ϩӯá ‫װ‬ϟЗÏʲϥϏԺ‫ױ‬?ЉЃ@ʤ:hјϒӑgʗǝĈիљˡæU|Obey{‫ײ‬í͟ĀԠղĈȆΎʏŴS`‫ײ‬ҳǱdˮgĆЏí͟ĀԠղĈȆΎGUObƔҦeU|ObЉˁS`C|9Ŝƾ8 «Cv\ϺӑgíʣőʗǝĈիљˡæU|Obey{‫ײ‬ǄĒǖ‫̪ה‬ăaֈϥǒ̼ahϥϦSdC¤ªÌփgϥϦԒȪHƔҦaB|ObǝƤSZ9ùƾh «Cv\ϺӑeFC`¤ªÌփgϥϦԒȪŘɿ¿©¾Ɗk¤ªÌփgϥϟΎʏe֛S`ǝƤU|9?˷·bѬ̲@ «Cv\Ϻӑŵ1̼h¤ªÌփϥϦSdCHDMSOΞŧǖ‫̈ה‬bíʣőʗǝĈիљÖΏaĶKp38 MAP¨ÐOSM1gղĈȆИǦ̼ah¤ªÌփHϥϦԒȪQ}|9OgϥϦԒȪ¿©¾̂zGeU|ZuјϒӑeFC`í͟ĀԠϥϦŘɿƿȆbS`Дz}|LaeAbg֛Ġԙo|ObeSZ9LaeAgÈÊaB|LAE1gղĈȆИǦ̼ahDMSOΞŧey|¤ªÌփgϥϦԒȪHԽOzdKd{8թšϿϝ̼ahDMSOdSav¤ªÌփgϥϦHԒȪQ}Z9p38 MAP¨ÐOSM1gղĈȆИǦ̼eFC`vLAE1ИǦ̼ah¤ªÌփgϥϦԒȪHԽOzdKd]Z9āÕgObGzDMSOΞŧbp38 MAP¨ÐИǦey|¤ªÌփgϥϦԒȪhLAE1úS`ԽI`
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Bioactivity of tenuazonic acid and its production control mechanism in Magnaporthe oryzae.
Choong-Soo Yun, Takayuki Motoyama, Yusi Hutamura, Hiroyuki Osada(Antibiotics Lab., RIKEN)- 76 —P-77 (O-3)˶Ӹí͟ĀԠŘɿƿȆbS`g Aospt3 g͜Ҧӽ̰ɧλְ÷ 1,2‫΀ײ‬ŉԥá 1,2‫ײ‬ȼÖƩӯ 1,2 ‫װ‬1 ɜȿǱ֤ÏļлЗ‫ײ‬2 տѼЗ‫ױ‬Քɘ‫ײ‬°¦Ìĥ‫ד‬с²«¤¢dŘɿgևӵʏHΉЉQ}`F{‫ײ‬ɲЗгȕahO}rae°¦Ì¡ÈŴĥ‫ד‬e֛ՠU|ղĈȆgӽ̰ӧ]`IZ9X}zղĈȆgИǦ̼ĕӱS‫ײ‬Ӫϝǒӽ̰ӧ]ZѬ̲8í͟ĀԠϥϦeɶ‫׃‬ØE|CK^GgƿȆHӷ^G{‫ײ‬XgÒ^e Aospt3 HȉǌS`CZ9јϒӑgí͟ĀԠϦϏ‫װ‬secondary metabolites‫׾‬āÖ SMs‫ױ‬h8̚ϧdϏԺGz̚ȘdϏԺraǮȺeZ{‫ײ‬XgŘɿ͓͜ӽ̂U|Obh‫ײ‬ϏԺϥϦwȎŁʏgӻπGzևӵaB|9XOa̤Згah‫שײ‬ӑeFM|˶Ӹd SMs ŘɿƿȆbS` Aospt3 eАЉS‫ײ‬SMs ϥϦŘɿ͓͜gӽ̂ЉЃbS`Згӧ]Z9̤Згah‫ײ‬SMs gÀ¥ÈϏԺbS`փϧC`C|9rV8ΔAospt3 eFM|փϥϦʏgӽ̰bփղĈȆÆÐ(kojAkojRkojT)gϿϝӽ̰ӧ]Z9XgѬ̲8ϥϦ։Hћ 100 ĨeǣŧS‫ײ‬rZղĈȆgϿϝÕ̀Hӷz}ZObGz‫ײ‬Aospt3 hփϥϦԪeŘɿS`C|ObH
̂zGbd]Z9јϒӑeFC`‫͔ײ‬:d SMs ϥϦŘɿe֛|ĀӪЃdƿȆbS` laeA HyKДz}`C|ObGz‫ײ‬Aospt3 b laeA g֛ĠeАЉSӽ̰ӧ]Z9ΔAospt3 eFM| laeA gղĈȆϿϝe^C`¬ÐÌӽ̰ӧ]ZѬ̲‫ײ‬ϿϝgÕ̀HЛԐQ}Z9Qze ΔAospt3ΔlaeA íևИǦ̼eFM|փϥϦʏӽ̰SZѬ̲‫ײ‬ΔlaeA bƛ͔e‫ײ‬փHϥϦQ}dCӪϝǒHɾz}Z9OgѬ̲Gz‫ײ‬Aospt3 b laeA hŅeփϥϦŘɿe֛ØS`C|ObĤzGbd]Z9ϝǌ ΔlaeA ®¢ÆÌ§ag spt3 ‫מ‬Ͽϝ‫ײ‬ΔAospt3 ®¢ÆÌ§ag laeA ‫מ‬ϿϝӧC‫ײ‬փϥϦŘɿeFM|
Aospt3 b laeA g²¤¢d֛Ġe^C`ӽ̰ӧ]`C|9
Functional analysis of Aospt3 secondary metabolic regulator
Masato Hirose , Moriyuki Kawauchi, Kazuhiro Iwashita(Hiroshima Univ, NRIB)


P-78
Emericella variecolor eFM|¤È¸ÌƙʣցѣgˍѤÔ͛֩к‫̮ײ‬ϨĚǱ‫ײ‬ΚɅȊҏ‫֡ײ‬պո̝ ‫̭װ‬Ǳ֤ÏӞ‫ױ‬јϒӑ Emericella variecolor Gzh‫ײ‬Ì¤ÌÌ II ƐȚĒeȣU|֠ȘΎʏwľϷʰŘΎʏ̚
U| variecolin‫ײ‬ѧҥͰʏΎʏϏԺ ophiobolin ‫׌‬gpG‫ײ‬astellatol‫ײ‬variculanol dcЮ:g¤È¸Ì
gſֵǝƤHdQ}`C|H‫ײ‬Xgϥƙʣe֛|ցѣhДz}`CdC9O}z E. variecolor ϩ̫¤È¸ÌϥƙʣղĈȆgӽ̂h‫׋ײ‬ӘdϥϏΎʏ̚U|ǲτ̚͜ŴƙϏgǱ։ϥϦw‫ײ‬ϥƙʣցѣ͜ҦwϥƙʣѫՂgˣǫey|˶Ӹ̚ϧŴƙϏgţŐevԵU|bҖEz}|9XOaʤ:h‫ײ‬O}z¤È¸ÌϥƙʣղĈȆgˍѤdzkeXg͜Ҧӽ̰eАʪSZ9
ɲЗгȕa§Æ³¦¬¾ÐÌӽ̰ӧ]Z E. variecolor g¬¾ÜeFC`‫ײ‬Aspergillus
clavatus ϩ̫g˺Д¤È¸Ìƙʣցѣ ophiobolin F synthase (AcOS) b‫מ‬CЋƛʏОUÌ¯ԺÐ§U|ղĈȆ͌ѤSZbO~‫ײ‬3 ^gĩӰղĈȆHӷŐQ}Z9O}zhCV}v‫ײ‬AcOS w
Phomopsis amygdali ϩ̫g¤È¸Ìƙʣցѣ fusicoccadiene synthase dcbƛ͔e‫ײ‬Њ֔ϒ»ÇµÉ©ÈíÇÌփƙʣպčb¤È¸ÌϣŴպčėWˀ^¿ÆցѣÐ§U|bëʙQ}|9ϝǌ‫ײ‬ƘղĈȆg͜Ҧӽ̰ӧDoK‫ײ‬ϴЮјϒӑ Aspergillus oryzae e`Ͽϝљ͓ьS‫ײ‬XgϥʣϏgő̰բu`C|9ŧE`‫ײ‬Ƙ¤È¸ÌƙʣցѣղĈȆgՔİeȉǌU|ˏȒĥ‫ד‬ցѣղĈȆҎe^C`v‫ײ‬ϴЮϿϝљ͓ьey|͜Ҧӽ̰ӧDëȒaB|9The search for sesterterpene synthases from Emericella variecolor
Takaaki Mitsuhashi, Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe(Graduate School of Pharmaceutical Sciences, Univ. of Tokyo)- 77 —P-79јϒӑϩ̫¿Ê¤È¸¬§ anditomin gϥƙʣЗг̮ϨĚǱ‫ײ‬ΚɅȊҏ‫֡ײ‬պո̝ ‫̭װ‬Ǳ֤ÏӞ‫ױ‬¿Ê¤È¸¬ §bh‫ײ‬¤È¸¬ §gպő͓՟̚U|ŴƙϏgѼЪaB{‫ײ‬јϒӑGzh͓՟Ǯ͔ʏdzkeϥϏΎʏeȞtǮˮg¿Ê¤È¸¬ §HǝƤQ}`C|9Aspergillus variecolor gϦϥU|
anditomin h‫ײ‬±Ê[2,2,2]Ì‫̚̿ם‬U|¿Ê¤È¸¬ §aB{‫ײ‬Ogϐϴd͓՟h‫ײ‬őȆŉDiels-Alder ƍʊѫ`ϥƙʣQ}|bëʙQ}`C|9O}rae‫ײ‬Ю:g͖ԢŴƙϏϧCZϥƙʣЗгHӧ}`IZÒ˷a‫ײ‬anditomin gϥƙʣղĈȆhŁK̂zGed]`CdC9ùƾʤ:h‫ײ‬anditominϥƙʣղĈȆgˍѤdzkeXg͜Ҧӽ̰ey|‫ײ‬Diels-Alder ƍʊʹDցѣ‫װ‬Diels-Alderase‫ױ‬gƛȒ‫ײ‬jC`hϥƙʣgŁȚӽ̂ЉˁSZ9rV‫ײ‬anditomin ϥϦӑg§Æ³¦¬¾ÐÌӽ̰ey{‫ײ‬13 ղĈȆGzd|ˏȒϥƙʣղĈȆÆÐӷŐSZ9¿Ê¤È¸¬ §gϥƙʣhÒҽe‫»ײ‬Ç §ƙʣցѣw¤È¸ÌϣŴցѣ
dcey|/Ṳ̈́‫͓̿ם‬ьƍʊ0bЮ:gփŴցѣdcey|/ĥ‫ד‬ƍʊ0g 2 ^eǱŖQ}|9ˏȒϥƙʣղĈȆҎє̺SZbO~‫ײ‬5 ^gղĈȆHṲ̈́‫͓̿ם‬ьeȜØU|vgbëʙQ}Z9XOa‫ײ‬O}z 5 ^gղĈȆϴЮјϒӑ Aspergillus oryzae e`ϿϝQWZbO~‫ײ‬ȪŀղĈȆϐϴЃe‫˶ׅ͟ײ‬Z
dŴƙϏH͌ŐQ}Z9ϝǌ‫ײ‬O}z˶ӸŴƙϏg͓՟ӽ̰բu|bbve8ĥ‫ד‬ƍʊʹDbҖEz}| 7 ^gղĈȆ‫ײ‬5 ^gṲ̈́‫͓̿ם‬ьղĈȆbŅϿϝQW|Oba‫ײ‬ĥ‫ד‬ցѣҎg͜Ҧӽ̰dzke
Diels-Alderase gˍѤӧ]`C|9
Biosynthetic studies on a fungal meroterpenoid anditomin
Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe(Graduate School of Pharmaceutical Sciences, Univ. of Tokyo)

P-80
Aspergillus fumigatus ϩ̫ pseurotin A ϥƙʣ͓͜gӽ̂Ǫ̈ɦÒչ‫̮ײ‬ϨĚǱ8ΚɅȊҏ8֡պո̝ ‫̭װ‬Ǳ֤Ӟ‫ױ‬Pseurotin A hјϒӑ Aspergillus fumigatus HĎփѣ̪ăÖaϦϥU|ŴƙϏaB{8Xg immunoglobulin
E ʰŘĕϧy{÷ĒgҢŉպgĎփѣ̪ăÖagʜ̸ʏeȜØS`C|bҖEz}`C|9XgýevСѫжԽgϥʣġբĕϧw¡Ìƙʣցѣg֠Șey|ʲЎӑΎʏс8Ǯ͔dϥϟΎʏˀ^9rZ8ϐϴd¶¤Ê²Êϣ͓՟‫̿ם‬eƠtZu8XgϥƙʣѫՂeҺƦHˀZ}|9ļӧЗгbS` 2007 ɘe
Turner zey{ A. fumigatus g¬¾ŉeưÒ^ȉǌU| polyketide synthase - non-ribosomal peptide
synthetase ӲƙǒցѣÐ§U|ղĈȆ(Afu8g00540)H pseurotin A gϥƙʣe֛ØU|ObHղĈȆИǦȔ‫כ‬ey]`ОQ}ZH8X}ā֢gԎѧdϥƙʣѫՂĥzGeQ}`CdC9̤Згah8јϒӑAspergillus oryzae e`ˏȒϥƙʣղĈȆÆÐgϴЮϿϝљ͓ьS8¶¤Ê²Êϣ‫̿ם‬ϥʣU|ցѣgƛȒbXgƍʊ¿©¾gӽ̂ЉЃbSZ9·ÐpTAex38pUSA8pAdeA8pPTRI Õg
AmyB µÊÀÐÐgÖΏe Afu8g005408hydrolase ղĈȆ(Afu8g00530  Afu8g00570)8P450 ղĈȆ
(Afu8g00560)8methyl transferase ղĈȆ(Afu8g00550)ÊÐ©ÌS`͓ьSZϿϝ·Ð A. oryzae
NSAR1 ̼eȪŀS8ɳԺՆ˔̼ƏɾSZ9ϝǌ8X}zԒȪǖ‫ה‬S8X}Y}gϥƙʣg¤¢µaӚвU|ĀԠϏgӽ̰ӧ]`C|9

Functional analysis of pseurotin A biosynthetic gene cluster in Aspergillus fumigatus
Koichiro Shiomura, Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe(Grad. School of Pharm. Sci., Univ. of Tokyo)- 78 —P-81јϒӑ Fusarium sp. FN080326 ̼ϩ̫³Ç¡Ì A ϥƙʣղĈȆÆÐgƛȒŧӟЊ͚ 1‫ײ‬Jun-Pil JANG2‫ײ‬Jae-Hyuk JANG2‫͛מײ‬Ģí 1‫ײ‬Jong Seog AHN2‫֗ײ‬Ϩӯá 1‫װ‬1 ϟЗÏCSRS‫ײ‬2KRIBB‫ױ‬³Ç¡Ì A hHѧҥgɳʝɳʣ֠ȘU|ϏԺbS` Fusarium sp. FN080326 ̼y{ſֵQ}ZĀԠϏaB{‫ײ‬Ã©Ðdïϣʏg˶Ӹοѣ‫̚̿ם‬U|9Fusarium ȷјϒӑgĀԠϏbS`Дz}`C|¡ÌϥƙʣÜ֚Ēe‫ײ‬XOGzϐʄЃdïϣʏ͓՟̚U|̤ŴƙϏeǫ˔Q}|bëʙQ}|9¡ÌvrZ‫͔ײ‬:dϥϏΎʏHǝƤQ}`C|ŴƙϏaB{‫ײ‬PKS-NRPS ­ ´Ç¢§ցѣeϩ̫U|¤¦Æ½ÌփŴƙϏaB|9̤Згh‫҃׌‬ĒţӱbΎʏŴƙϏg‫מ‬ϥϦЉЃe‫ײ‬³Ç¡ÌϥƙʣղĈȆÆÐgƛȒb8¤¦Æ½ÌփŴƙϏGzy{Ӳֳdϣ͓՟̚U|ŴƙϏngǫ˔ƍ
ʊe֛|ցѣղĈȆg͜Ҧӽ̰ӧ]Z9rVh‫ײ‬³Ç¡ÌϥϦӑ FN080326 ̼g§Æ³¦¬¾ӽ̰ӧC‫ײ‬í͟ĀԠղĈȆÆÐˍѤSZ9XgѬ̲‫ײ‬PKS-NRPS ­ ´Ç¢§ցѣղĈȆh¬¾Üe 1 ղĈȆgsaB{‫̘ײ‬ՔǝƤQ
}Z¡ÌϥƙʣʹD­ ´Ç¢§ցѣղĈȆ eqxS‫װ‬Kakule TB, et al. 2013 ACS Chem Biol
8:1549-1557‫ױ‬bֿɓe‫מ‬CЋƛʏОSZ9ƥՏgղĈȆ͓ʣv eqx ÆÐgX}bւČS`F{‫ײ‬
XOa‫̤ײ‬ղĈȆÆÐ³Ç¡ÌϥƙʣղĈȆ‫װ‬fsa‫ױ‬ÆÐgĩӰbS`ӽ̰բu|O
bbSZ9fsa ÆÐeưÒƠr}|¦Ê¾ P450 ղĈȆ fsa9 H¡ÌGz³Ç¡Ìngǫ˔ʹDցѣղĈȆg̚ťĩӰaB{‫ײ‬ϝǌ‫ײ‬ղĈȋЃµÊÐ¡ey{̤ղĈȆgϥƙʣng֛Ø͌ԈU|bbve‫ײ‬ցͯϿϝљϧC`͜Ҧӽ̰ӧ]`C|9
Identification of fusarisetin A biosynthetic gene cluster of Fusarium sp. FN080326
Naoki Kato1, Jun-Pil Jang2, Jae-Hyuk Jang2, Shunji Takahashi1, Jong Seog Ahn2, Hiroyuki Osada 1(1RIKEN CSRS, 2KRIBB)

P-82
јϒӑ Fusarium sp. RK97-94 gÈÆÌϥƙʣղĈȆÆÐÜg¿¡ÈǗՆЫցѣղĈȆ luc1 b¦ÆÌ»ÐÐղĈȆ luc4 g͜Ҧӽ̰ŧӟҒ 1,2, ̤ȹ‫מ‬ə 1, ɧϨΌ 1, ֓Ħ‫מ‬ʉ 2, ֗Ϩӯá 2 ‫װ‬1 ϟЗÏʲϥϏԺ‫ײ‬2 ̭õϟЧǱ‫ױ‬?ЉЃ@ÈÆÌ(1)hјϒӑ Fusarium sp. RK97-94 HϥϦU|ĎőȆŴƙϏaB{‫ײ‬HʰŘղĈȆ p53 g×ΎʏŴѧҥaѧҥƥ̠֠ȘΎʏОU91 ϥϦӑhƛ̈eϣϒÐ¤ÈH֙ϣS`C|‫҃׌‬ĒNG-391(2)ÞӵĀԠϏbS`ϥϦU|9ʤ:h 1 b 2 gϥƙʣeʇ‫׆‬d PKS-NRPS ӥƙցѣղĈȆ luc5ÊÐ©ÌS‫ײ‬XgՔİe 8 ղĈȆGzd| 1 gˏȒϥƙʣղĈȆÆÐHȉǌS`C|Ob̂zGeS`C|9ùƾ‫¡¿ײ‬ÈǗՆЫցѣղĈȆ luc1 b¦ÆÌ»ÐÐղĈȆ luc4 g͜Ҧӽ̰ӧ]Z9?˷·bѬ̲@rV luc4 ղĈȆИǦ̼ĕӱS‫ײ‬ĀԠϏӽ̰SZ9ИǦ̼ah 2 gϥϦʏhֈϥ̼bͱoǫŴHӷz}dG]ZH‫ײ‬1 gϥϦʏhǱɔeĎÖSZZu‫ײ‬Luc4 h 1 gϥƙʣeϐϴЃe֛ØS`C|ObHОQ}Z9͟e‫ײ‬ѧҥǭngˋŐe֛ØU|¦ÆÌ»ÐÐbS`g͜Ҧˀ^GcDGe^
C`ӽ̰SZH‫ײ‬ղĈȆИǦ̼bֈϥǒ̼a 1 b 2 gѧҥǭˋŐҦeǫŴhdK‫ײ‬Luc4 hѧҥǭngˋŐeh֛ØSdCObHОƮQ}Z9͟e luc1 ղĈȆИǦ̼ĕӱS‫ײ‬ĀԠϏő̰SZ9ИǦ̼h‫ײ‬1b 2 gϥϦҦǶC‫ײ‬1 gҪ¿¡ÈŴĒ(3)ÞӵĀԠϏbS`ϥϦU|yDed]ZObGz‫ײ‬Luc1 HÈºÈǗg¿¡ÈŴe֛ØS`C|ObĤzGed]Z92 gҪ¿¡ÈŴĒHÞӵ²ÐbS`ӻȡQ}dG]ZObGz‫ײ‬ÈºÈǗg¿¡ÈŴHϣϒÐ¤Èɳʣeɶ‫׃‬U|ObHОƮQ}Z9ùƾgѬ̲vbe‫ײ‬1 gϥƙʣѫՂˏȒSZ9

Functional analysis of the methyltransferase gene luc1 and the transporter gene luc4 in the lucilactaene
biosynthesis gene cluster of the producing fungus Fusarium sp. RK97-94
Sho Kato1,2, Takayuki Motoyama1, Hiroshi Hirota1, Takashi Kamakura2, Hiroyuki Osada1(1Antibiotics Lab., RIKEN, 2Tokyo Univ. of Sci.)- 79 —P-83‫ש‬ӑ hstD ИǦey|í͟ĀԠϏϥϦљgΎʏŴ΀ŉԥá 1‫ ײ‬2‫ײ‬ԧΒŗ̳Ȇ 2‫ײ‬ȼÖƩӯ 1‫ ײ‬2 (1 ɜȿǱ֤ÏļлЗ‫ײ‬2 տѼЗ)?Ҥ̍@јϒӑh‫ײ‬ǮЮǮ͔dí͟ĀԠϏϥϦU|ρ‫ײ‬XgŘɿ͓͜HΉЉQ}`C|9Ogí͟ĀԠϏϥϦŘɿe‫ײ‬°¦Ìĥ‫ד‬ղĈȆH֛ØU|ObHëʙQ}ZObGz‫ײ‬ʤ:hO}rae‫ש‬ӑgŁ
Histone deacetylase e^C`͜Ҧӽ̰ӧ]Z9XgѬ̲8ӑ‫׌‬ϐϴЃ sirtuin aB| hstD H‫ײ‬laeA gŘɿúSí͟ĀԠϏϥϦbőŴŘɿU|ì˶ӸeӷŐS`C|9̤Згah‫ײ‬hstD ey|í͟ĀԠϏϥϦŘɿe^C`̕d|ϟӽU|ObЉЃe‫ ¼ײ‬ÊÉ ϧCZղĈȆϿϝӽ̰Ûke
LC-Q/TOF-MS ĘϧSZĀԠϏӽ̰ey{ɜыeí͟ĀԠϏϥϦљe^C`ӽ̰ӧ]Z9?˷·ÏѬ̲@‫ש‬ӑ hstD ИǦ̼փϥϦǖǎa 4 ˻֚ΙĒǖ‫ה‬ɼ‫ײ‬ӑĒGz RNA ʸŐS¼ 
ÊÉ ӽ̰Ȕ˸SZ9Control ̼bͱՈS ΔhstD ̼a‫ײ‬ϿϝHƃ֦ϙ 5%a 2 ĨāÕǫŴSZղĈȆʸŐSZѬ̲‫ײ‬ϿϝÕ̀B|ChĎÖSZղĈȆH‫ײ‬X}Y} 228 ħb 160 ħʸŐQ}Z9ϿϝHÕ̀S
ZղĈȆeh‫֛ײ‬ՠU|ϥϦϏԺH×̂g PKS w NRPS Ð§U|ղĈȆHƠr}`CZ9Ͽϝǫů
H̚]ZղĈȆҎe^C`‫ײ‬FungiFun ϧC` FunCat ey|͜Ҧő‫׌‬ӧ]Z9XgѬ̲‫ײ‬ϿϝÕ̀S
Zvgeh‫[ײ‬secondary metabolism]w[detoxification]eő‫׌‬Q}|ղĈȆHǮKƠr}`C|ìĤzGbd]Z9
ѶC`‫שײ‬ӑ hstD ИǦ̼փϥϦǖǎa 4 ˻֚ΙĒǖ‫ה‬ӧC‫ײ‬ǖǎс։g SM ʸŐΙ(methanol : dichloromethane : ethyl acetate=1:2:3‫ײ‬1% formic acid)aőսS‫ײ‬ɾz}Z̚͜ȸgʣőe^C`‫ײ‬
LC-Q/TOF-MS ϧC`ӽ̰ӧ]Z9XgѬ̲‫ײ‬Ì¦ÊÐÈ̼ah͌ŐQ}dCvgƠu‫ײ‬ǮKgĀԠϏԺgϥϦHÕ̀S`C|ObĤzGbd]Z9āÕgѬ̲ey{‫שײ‬ӑ hstD gИǦey{í͟ĀԠϏϥϦљHΎʏŴQ}|ìHОƮQ}Z9
Activation of secondary metabolism in the hstD disruptant of Aspergillus oryzae.
Moriyuki Kawauchi1‫ײ‬2‫ ײ‬Rieko Toyoura 2‫ ײ‬Kazuhiro Iwashita1‫ ײ‬2(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB)

P-84‫ש‬ӑ Aspergillus oryzae ϩ̫ µ III ǒ»Ç
3-acetyl-6-alkyl- α-pyrone gϥʣ§ƙʣցѣ CsyB ey|͛ĺ Ԕ 18‫͛מ‬Ȑ̂ 18‫מ‬ǉ ȗ 18‫׆‬ϨźȨ 18ŵ̤ŲjO 28ӟð ų 1‫װ‬ȼʪŸЧǱÏӞ8̭Ǳ֤ÏՎϥЧÏʊϥɈ‫ױ‬ µ III ǒ»Ç §ƙʣցѣaB| CsyB  A. oryzae ÜaϿϝQW|bɳԺՆ˔Ēgǖ‫ה‬ΙÜe
3-acetyl-α-pyrone ‫̿ם‬g csypyrone B (CPB) ‫׌‬HϥϦQ}|9CPB ‫׌‬h fatty acyl-CoA HÐÐbd{ÈÈĮ֔v^ pre CPB gɳʣɼ8țÞŉaփŴQ}|ObaϥʣU|ObHëʙQ}Z9ùƾ8Ǳ
ҰӑaϿϝQWZbIeɾz}ZϥʣϏg͓՟ӽ̰8Fyk in vitro ey|ƍʊӽ̰ӧ]Zga8ǝƤ
U|9csyB ղĈȆ pET22b eÊÐ©Ìɼ8BL21(DE3) ̼e`Ò̋8ĎΦÖaԒȪǖ‫ה‬SZ9ԒȪǖ‫ה‬Ι HPLC ő̰SZbO~8Ì¦ÊÐÈehdC²ÐӲˮ͌ŐSZ9͓՟ӽ̰ӧ]ZѬ̲8C15 gÈÈFykÈ©ÈĮ֔ 6 čev^ pre CPB ‫҃׌‬ĒHÞϥʣϏaB|Ob̂zGeSZ9in
vitro ey|ӽ̰ah8pColdTF eÊÐ©ÌS8ƛ͔eԒȪǖ‫ה‬ɼ8Ni-NTA єӱSZӥƙÌ¯ϧCZ9XgѬ̲8butyryl-CoA Fyk malonyl-CoA8acetoacetyl-CoA ǗԺbS`ϧCZ̈e C3 µÊ²ÈĮ֔g pre CPB (pre CPB-C3)gϥʣԺ։ő̰ey{ЛԐSZ9rZ8ÐÐ hexanoyl-CoA8
octanoyl-CoA eǫ̕SZbIe pre CPB-C5 Fyk-C7 eȣʊU|²ÐX}Y}ЛԐSZ9āÕgOb
Gz8CsyB h fatty acyl-CoA8malonyl-CoA Fyk acetoacetyl-CoA ǗԺbS` 3-acetyl-6-alkyl-α-pyrone ϥʣU|vgbѬԜSZ9
Production of 3-acetyl-6-alkyl-α-pyrones by a type III polyketide synthase CsyB from Aspergillus oryzae
Makoto Hashimoto1, Hiroaki Takahashi1, Tsukasa Koen1, Chihiro Suda1, Katsuhiko Kitamoto2, Isao Fujii1(1School of Pharmacy, Iwate Medical Univ., 2Dept. of Biotechnol., The Univ. of Tokyo)- 80 —P-85
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DNA double-strand breaks mediated targeted gene replacement in the rice blast fungus
Tetsuya Younomaru1, Takayuki Arazoe1, Shuichi Ohsato1, Tsutomu Arie2, Shigeru Kuwata1(1Sch. Agric., Meiji Univ., 2Grad. Sch. Agric. Sci., Tokyo Univ. of Agric. & Tech.)P-86ЎӑϧCZ Roxithromycin g˶ӸĕϧπgˍѤχǏӈđ‫ײ‬Жð̎‫ײ‬ѡ՛ċ΂‫ײ‬ĦˀəƗ‫ײ‬ӏƆíÔϫ‫ײ‬ǸҾ‫װ‬ʣɅ‫ױ‬ʒ‫֓ײ‬Ħ‫מ‬ʉ‫̭װ‬ϟǱ֤ϟɈÏʊϥЧ‫ױ‬«Cv\Ϻӑ(Magnaporthe oryzae)h «eʜ̸S`ϺȘɫIԽOU͋ϏϺƆјϒӑaB|9̤ӑhțÞ͋ϏŉngĞŀeFC`ʜ̸ϐϴЃdƺȑaB|þАƺɳʣU|9̤ӑgþАƺɳʣh,Ў̽ϥϏgѧҥőŴg̘vſўd͓͜gÒ^aB{,ѧҥőŴehǮKgղĈȆH֛ØU|ZuƘЮgѧҥŉƿȆ֠ȘU|ŴƙϏeȣS`֏˨eƍʊU|9̤ЗгhOgþАƺϧC`ƘЮŴƙϏg˶ӸĕϧˍѤS,Xgĕϧ͜ĕӽ̂U|ObЉЃbS`C|9̤ӑe͔:dŴƙϏĕϧQWZbO~,14 ƫϣ¼ÊÆ §љʲϥϏԺaB| Roxithromycin(RXM)
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Mayu Kumasaka, Akira Ishii, Shinji Kamisuki, Kouji Kuramochi, Fumio Sugawara, Megumi Narukawa-Nara,
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C`8ChSte20 ͞˗̼ahֈϥ̼bƛ͔aB]ZH‫ײ‬ChCla4 ͞˗̼ahɳʝϴɓgőϥȆHɳʣQ}Z9
ChSte20 ͞˗̼hʜ̸թЬeFC`ֈϥ̼b‫׋‬Әdɋhӷz}dG]ZÒ˷‫ײ‬ChCla4 ͞˗̼ahþАƺgɳʣeFC`ӘSCĎÖHԐuz}‫ײ‬ʜ̸ҦťǶ]`CZ9̚ʏϥͪe^C`h‫ײ‬ÚղĈȆ͞˗̼aįȆƻͭgϿիgեɨHԐuz}‫ײ‬ChSte20 ͞˗̼ahȆƻFykȆƻҥȆHɳʣQ}ZÒ˷‫ײ‬ChCla4 ͞˗̼ahpbcɳʣQ}Vֲʏ×ЭaB]Z9O}zgѬ̲Gz‫^׷ײ‬g PAK-like ¨ÐeFC`͜ҦőŴHԐuz}Z9Ud\‫ײ‬Þe ChCla4 HςʏÏ̚ʏÙĀg͔:dɳʝɳʣ֪ͫeFC`ևӵdɷŢˀ^Ò˷‫ײ‬ChSte20 e^C`h̚ʏϥͪgŘɿe֛ØU|H‫ײ‬ʇVSvʇ‫׆‬ahdCObĤzGbd]Z9Characterization of ChSte20 and ChCla4 genes in Cochliobolus heterostrophus
Yuki Kitade1, Kosuke Izumitsu2, Takuya Sumita1, Satoshi Yutani1, Chihiro Tanaka1(1Grad. Sch. of Agriculture, Kyoto Univ., 2Sch.of Environmental Science,Univ. of Shiga Pref.)P-92Ç‫׌‬οϸϺӑeFM| Ras GTPase ΎʏŴÌ¯Ժ CoIra1 h CoRas2 úS`cAMP ¨ÈĈիѫՂb CoMekk1-Cmk1 MAPK ѫՂgÕΏŘɿƿȆbS`֛ØU|ƆϨ Ը‫ײ‬àģɦá ‫װ‬õɡǱ֤Ïϥϣ‫ױ‬ʤ:hÇ‫׌‬οϸϺӑeFM|ʜ̸ƺȑgɳʝɳʣwϺƆʏe֛ØU| Ras GTPase ΎʏŴÌ¯ԺÐ§U| CoIRA1 ƛȒS`C|9̤Згah CoIra1 ey{ŘɿQ}|¨ÈĈիѫՂӽ̰SZ
9rV,Őӄցͯg Ira1/2 h Ras1/2 úS`ѧҥŉ cAMP ։ŘɿU|ObGz,coira1 ̼gѧҥŉ cAMP։ΧȒSZ9XgѬ̲,coira1 ̼g cAMP ։hֈϥ̼y{ǣŧS,CoIra1 hѧҥŉ cAMP ։ŘɿU|
ObHОƮQ}Z9͟e CoIRA1 b CoRAS2 g֛Ġ̂zGeU|ρecoras2 ̼ĕŐS,Ӫϝǒ͌ԁ
SZ9XgѬ̲, coras2 ̼hϿӄ͞˗ОS,CoRAS2 hҥȆϿӄe֛ØU|ObHОƮQ}Z9͟ e,CoRas2 bʐɓΎʏǒ CoRAS2 e RFP SZӑ̼ĕŐS,CoRas2 gȳǌͱՈSZ9XgѬ̲,ֈϥ̼ӑјeFC` CoRas2 hѧҥҲwѧҥԺeȳǌSZH,ʐɓΎʏǒ CoRAS2 hѧҥҲe‫׋‬Әd ȳ ǌ  О S Z 9 r Z ,  coira1 ̼ a h CoRas2 h ѧ ҥ Ҳ e ‫ ׋‬Ә d ȳ ǌ  О S Z 9 O g O b G z
CoIra1 h CoRas2 e Ԫ g Ř ɿ  U | Ɣ Ҧ ʏ H О Ʈ Q } Z 9 Q z e , CoRas2 H CoMekk1-Cmk1
MAPK ѫՂe֛ØU|GʐɓΎʏǒg CoRAS2 b CoMEKK1 Ȫŀ̼gӪϝǒey{͌ԁSZ9Xg
Ѭ ̲ Δcoras2 ̼ngʐɓΎʏǒ CoMEKK1 Ȫŀӑ̼hҥȆϿӄS,Ò˷, Δcomekk1 ̼ n g ʐ ɓ Ύ ʏ ǒ
CoRAS2 Ȫŀӑ̼hҥȆϿӄg͞˗ОUObGz,CoRas2 h CoMekk1-Cmk1 ѫՂgÕΏƿȆaB|bОƮQ}Z9āÕGzCoIra1 h CoRas2 úS` cAMP ¨ÈĈիѫՂb CoMekk1-Cmk1
MAPK ѫՂe֛ØU|ObHОƮQ}Z9
Ras GTPase activating protein CoIRA1 is involved in cAMP signaling transduction pathway and
CoMekk1-Cmk1 MAPK pathway as an upstream regulatory factor in Colletotrichum orbiculare.
Ken Harata Yasuyuki Kubo
(Life and Environmental Science, Univ. of Kyoto Prefectual)- 84 —P-93«Cv\Ϻӑ¼

È MoCV1 b MoCV1-B g¬¾͓՟gͱՈ‫ן‬ðճȆ‫ײ‬ΒȹĢÒ‫ײ‬ՖϨѠƕ‫ײ‬ŧӟĸ‫̨̡ײ‬ĸֆ‫ײ‬УƆ˨ӧ‫̚ײ‬͹ť‫ײ‬ȢȻʅ‫͉ײ‬ȹӯĻ(ՎɈǱ֤ÏՎ)
Magnaporthe oryzae chrysovirus 1 (MoCV1) h 5 ̤g dsRNA ¬¾̚U|¼  Èa‫ײ‬țÞg« C v \ Ϻ ӑ e ϥ ҡ ֠ Ș F y k Ϻ Ɔ ť g Ď Ö  ɫ I Խ O U 9 O g MoCV1 b Ћ ƛ ʏ  ˀ ^Magnaporthe oryzae chrysovirus 1-B (MoCV1-B)Hʜ̸SZ «Cv\Ϻӑ̼h‫ײ‬MoCV1 ʜ̸̼eͱo`QzeϥҡHŨ|9O}zg Èh̠֗ǖ‫ה‬ey]`ѧҥǭeȉǌSɾ|ObϐʄbS‫ײ‬Xg֬ È͓՟Ì¯Ժg ORF3(dsRNA3 HÐ§)b ORF4(dsRNA4 HÐ§)HX}Y} 83kDa,
85kDa Gz 58kDa, 70kDa dceϐϴЃeպőőӽQ}|9Ogőӽ͓͜gӽ̂ЉЃbS`‫ײ‬MoCV1
b MoCV1-B g¬¾͓՟ƊkÐ§Q}|Ì¯Ժe^C`ͱՈ͌ԁӧ]Z9MoCV1 b
MoCV1-B g Ƙ  ¬ ¾ ͓ ՟ g ̽ փ Ћ ƛ ʏ h ‫ ײ‬dsRNA1(79.6%), dsRNA2(76.3%), dsRNA3(71.3%),
dsRNA4(72.4%), dsRNA5(96.2%) ‫ ײ‬Ł Ē g  Ì ¯  Ժ g ƛ Ò ʏ h ‫ ײ‬ORF1(92.5%), ORF2(80.1%),
ORF3(87.8%), ORF4(89.4%), ORF5(97.1%)aB]Z9Ogŉ ORF2 g N ̣л(ћ 200aa)h 51.1%‫ײ‬ORF3
g C ̣л(ћ 200aa)h 34.1‫ׯ‬bĎCƛÒʏОS‫ײ‬Og C ̣лhպőőӽey]`֥ƈQ}|‫ׇ‬ǕbÒҹSZ9ORF1, ORF4 eFC`hƛÒʏgī{hӷz}dG]Z9ǱҰӑwցͯϧCZ OFR3, ORF4gϴЮÌ¯ԺϿϝљahőӽQ}ZÌ¯Ժh͌ŐQ}V‫ײ‬Ł֗gÌ¯ԺgsH͌ŐQ}|
ObGz‫ײ‬ӻȡQ}ZµÊ¢ÌhCv\ϺӑgѧҥŉaϐϴЃeϥT`C|ƔҦʏHОƮQ}Z9

Comparison of genome structures between Magnaporthe oryzae chrysovirus 1 and Magnaporthe oryzae
chrysovirus 1-B
Ryoko Takai, Syun-ichi Urayama, Hirofumi Sakoda,Yu Katoh, Yuri Kimura, Toshiyuki Fukuhara, Tsutomu Arie,
Tohru Teraoka, Hiromitsu Moriyama (Faculty of Agriculture, Tokyo Univ. of Agri. & Tech)

P-94͋ϏϺƆʏ Alternaria alternata eFM| global regulator LaeA g͜Ҧӽ̰‫מ‬ȱƩȔ 1‫ײ‬Իֽ̡Ň 1‫̤˞ײ‬Ė̂ 2‫ײ‬ЖƆô 1‫̹͋ײ‬ȭʉ 2‫ֶײ‬Έ̻í 3‫ײ‬ĿϚǗÒ̝ 1‫װ‬1 ‫ף‬ƏǱÏ2 ƜǱ֤ϥՎÏ3 ‫ף‬ǱŸ‫ױ‬
methyltransferase Ð§U| LaeA ղĈȆh Aspergillus nidulans eFC`ӷŐQ}8global regulator bS`Ӳˮ
gí͟ĀԠϦϏ²«¤¢eŘɿS`C|bҖEz}`C|‫¦¼¦׳‬ÈÐ¨ÇӉ̶Ϻӑ(Alternaria alternata tomato pathotype, Ӊ̶Ϻӑ) gŁ¬¾§Æ³¦ÐÌʗǝy{‫ײ‬LaeA ¹ÀÊaB| AaLAEA ƛȒSZ. rZ‫׬¡ ײ‬ϜϺӑ (A. alternata strawberry pathotype) FykÇÌ˲πӖӗϺӑ (A.
alternata apple pathotype) eFC`v‫ײ‬ƛ͔e LaeA ¹ÀÊƛȒSZ‫̤׳‬ղĈȆ¬¢¦ (KO) ̼‫ײ‬X}Y}g A. alternata ֈϥ̼bͱՈSZѬ̲‫ײ‬3 ϺƆǒŁ`eFC`ӑјʣ֗FykÊ©ÐɳʝeɋϴHԐ
uz}Z. Qze‫ײ‬O}z KO ̼gͰѣϥϦҦ FykϺƆʏh‫ײ‬ֈϥ̼bͱՈS`ӘSKĎÖS`CZ‫׳‬āÕgѬ̲y{‫ײ‬LaeA hțÞϐϴЃͰѣĜȉϺƆӑeFC`‫ײ‬ɳʝɳʣ‫Ͱײ‬ѣϥϦFykϺƆʏɜыeŘɿS`C|ObHОƮQ}Z‫׳‬Functional analysis of the global regulator LaeA homologues in the plant pathogenic Alternaria alternata
Kazumi Takao1, Yasunori Akagi1, Yoshiaki Harimoto2, Toru Ishihara1, Takashi Tsuge2, Eiji Nanba3, Motoichiro
Kodama1(1Fac. Agric., 3Fac. Medicine, Tottori Univ., 2Grad. Sch. Bioagric. Sci., Nagoya Univ.)- 85 —P-95
μҿGkϺӑgÐ¦³Ð֛ՠղĈȆ BcAtg1 g͜Ҧӽ̰ďϨżå 1‫΅ײ‬΍ɬđ 2‫ײ‬ϨÜźȨ 1 ‫װ‬1 õǱÏ֤ÏՎ‫ײ‬2 ίԴЍǱÏϣǢЧȋ‫ױ‬Ð¦³ÐhЎ̽ϥϏeģȉQ}ZѧҥŉagҷɌ͓ʣʣőgÞӵdőӽљgÒ^aB{‫ײ‬јϒӑ‫׌‬eFC`hҥȆɳʣw‫ͳײ‬Üӑјɳʣe֛ØU|ObHǝƤQ}`C|9rZՔɘ‫͋ײ‬ϏϺƆʏјϒӑeFC`hțÞngϺƆʏbg֛ՠʏeFC`vΉЉֱu`C|9μҿGkϺӑ Botrytis cinerea hǮϑʏgևӵ͋ϏϺƆʏјϒӑaB{‫ײ‬ƛӑgÞӵdʜ̸Ϋbd|ǱǒőϥȆb‫̚ײ‬ʏϥͪeFM|֯ʏƺ ȑ b S ` g ͜ Ҧ  v ^ ȫ ǒ ő ϥ Ȇ g í Ю ‫ ׌‬g ő ϥ ҥ Ȇ  ɳ ʣ U | 9 ù ƾ ʤ : h ‫ ײ‬Ő ӄ ց ͯ
Saccharomyces  cerevisiae eFC`Ð¦³Ðg֙ǿeʇӵaB|bQ}|ÇÌ‫״‬É©Ì¨ÐÐ§U|ղĈȆ Atg1 gμҿGkϺӑeFM|ÈÊղĈȆ BcAtg1 ƛȒS‫ײ‬XgղĈȆИǦ̼ĕŐSZ9BcAtg1 ИǦ̼hֈϥǒ̼bͱoɗ̯ǖǎÕagͳÜӑјɳʣHĎÖS‫ײ‬ӑƑÕeɳʣQ}ZǱǒőϥȆ̷hӘSKԭɮd͔ЋƣSZ9őϥҥȆgɳʣˮԙ̺SZbO~‫ײ‬BcAtg1 ИǦ̼e
FC`hǱǒőϥȆgɳʣˮHŤЃeĎÖS`C|Ob‫ײ‬QzeȫǒőϥȆgɳʣˮv̚ʚeĎÖS`C|ObĤzGbd]Z9ǱǒőϥȆ̷gɳʣ‫׉‬ɢeFC` BcAtg1 ИǦ̼bֈϥǒ̼g֚e‫׋‬ӘdɋϴhԐuz}dG]ZZu‫ײ‬BcAtg1 ИǦ̼eFM|ǱǒőϥȆɳʣˮgŤЃdĎÖgÞdӵƿh‫ײ‬ǱǒőϥȆ̷gϿիHӘSK˗d}|Obey|bҖEz}|9ϝǌ‫ײ‬BcAtg1 ИǦ̼gțÞngϺƆʏe֛U|ƘЮԊ‫כ‬ӧ]`F{‫̤ײ‬ϿӪeFC`hO}zgѬ̲e^C`vǝƤU|9

Characterization of the autophagy-related gene BcAtg1 in Botrytis cinerea
Takuya Sumita1, Kosuke Izumitsu2, Chihiro Tanaka1(1Grad. Sch. of Agriculture, Kyoto Univ., 2Sch. of Environmental Science, Univ. of Shiga Pref.)P-96 (O-5)
Ç‫׌‬οϸϺӑeFM|Őӄցͯ RAM «¢¦ËÐ͓ʣƿȆ CoPag1 h͋ϏϐϴЃ¨ÈƐȚúSZþАƺɳʣe֛ØU|ȫϚџĀ‫ײ‬Ǐƒ ͤ‫ײ‬àģɦá ‫װ‬õռɡǱ֤Ïϥϣ‫ױ‬ǮKg͋ϏϺƆјϒӑeFC`þАƺɳʣhϺƆʏϿϝeʇ‫׆‬aB{‫͋ײ‬ϏӪ‫׀‬gϏԺwϏϟЃɳϒg¨ÈʜДS`ӧ}|9ʤ:hO}rae͋ϏϺƆјϒӑÇ‫׌‬οϸϺӑeFC` CoKEL2 HþАƺgɳʝɳʣFykѧҥ͐ʏŘɿe֛ØU|Ob̂zGeS`C|9rZ‫ײ‬cokel2Δ h÷ɈǗԺÕaϴɓɳʝþАƺɳʣU|HțÞӗÕah͢ɓþАƺɳʣU|ObGz‫ײ‬þАƺɳʣe֛ØU|͋ϏϐϴЃ¨ÈƐȚѫՂgȉǌОƮS`IZ9̤Згah͋ϏϐϴЃ¨ÈƐȚѫՂe֛ØU|ղĈȆƛ
ȒU|Zue‫ײ‬ÆÌ ¾ղĈȆ˅ŀey{ƏɾSZɳʝɳʣÏϺƆʏ͞˗ CoKEL2 íևǫϴ̼ 6 ӑ̼e^C`¬¾ÐÌӽ̰ӧCǫϴղĈȆgƛȒԊsZ9XgѬ̲‫ײ‬ǫϴղĈȆbS`Őӄցͯgɳʝɳʣe֛ØU| RAM (regulation of Ace2p activity and cellular morphogenesis)«¢¦ËÐg͓ʣƿȆ
Pag1 (Tao3)bЋƛʏv^ CoPAG1 ƛȒSZ9copag1Δcokel2Δ ĕŐSZbO~‫÷ײ‬ɈǗԺÕ[MadKțÞӗÕeFC`vϴɓɳʝþАƺɳʣS‫ײ‬ϺƆʏ͞˗Hӷz}Z9OgѬ̲Gz CoPAG1 HþАƺɳʣe֛ØU|͋ϏϐϴЃ¨ÈƐȚѫՂg͓ʣƿȆaB|ƔҦʏHОƮQ}Z9͟e CoPag1::RFP gѧҥŉȳǌӻȡSZѬ̲‫ײ‬Æ §ÆÕahҥȆFykþАƺgѧҥԺeӤĽHӷz}‫ײ‬ϿӄчehӻȡQ}dG]Z9Ò˷‫ײ‬țÞӗÕahҥȆ‫ײ‬Ͽӄч‫̢ײ‬ʣψþАƺgՌջОUyDeӤĽHӻȡQ}‫ײ‬CoPag1::RFP hțÞӗÕeFM|þАƺgɳʝőŴ̈egsѧҥҲƥՏeȳǌU|ƔҦʏHОƮQ}
Z9
Colletotrichum orbiculare CoPag1, a component of RAM network in Saccharomyces cerevisiae, is involved inappressorium development triggered by plant-derived signals
Sayo Kodama, Ayumu Sakaguchi, Yasuyuki Kubo
(Graduate School of Life and Environmental Sciences, Kyoto Prefectural Univ.)- 86 —P-97¦¼¦ÈÐ¨ÇӉ̶ϺӑHģ̚U|ϺƆʏ̸ҿĒg͓՟ӽ̰Իֽ̡Ň 1‫ײ‬ǮԴ͢ъ 2‫̹͋ײ‬ȭʉ 3‫ֶײ‬Έ̻í 4‫ײ‬ĿϚǗÒ̝ 1‫װ‬1 ‫ף‬ƏǱՎÏ2 ȻȹǱϟÏ3 ƜǱ֤ϥՎÏ4 ‫ף‬ƏŸ‫ױ‬¦¼¦ÈÐ¨ÇӉ̶Ϻӑ (Alternaria alternata tomato pathotype) h‫ײ‬țÞϐϴЃ AAL ͰѣϥϦU|Obey{‫ײ‬ϐȒg¦¼¦ƪЮegsӘSCǦͦϺ˲ɫIԽOU9̤Ͱѣh‫͓ײ‬՟Ѓe Giberella ЮgϥϦU|¼ ¦Ì ³À©Ìb‫׌‬ČS`F{‫ײ‬X}Y}gϥƙʣe֛ØU|ղĈȆҎ‫װ‬AAL Ͱѣ‫׾‬ALT ÆÐ‫ײ‬³À©Ì‫׾‬FUM ÆÐ‫ױ‬v‫׌‬ČS`C|9̤ӑeFM|¬¾ÐÌӽ̰FykղĈȆ͜Ҧӽ̰ey{‫ײ‬ALT ÆÐղĈȆgpbcH AAL Ͱѣϥƙʣe֛ØS`C|
ObĤzGbd]`C|9ALT ÆÐh‫̤ײ‬ӑHϐϴЃeģ̚U| 1 Mb conditionally dispensable
chromosome (CDC) eɣãS`F{‫ײ‬CDC g̚ςey{̤ӑgϺƆʏHͻȒQ}|Zu‫ ̤ײ‬CDC h/ϺƆʏ̸ҿĒ0aB|bsdQ}|9CDC gÐÌӽ̰‫ײ‬optical mapping Fyk rare cutting enzyme (NotI)
ey| RFLP ӽ̰ey{‫̸̤ײ‬ҿĒHƛҮ̸ҿĒ (isochromosome) ͓՟̚U|ƔҦʏHОƮQ}Z9Q
ze‫ײ‬CDC ÕgÌ¦Ê¿Fyk¤Ê¿Քİ‫ׇ‬ǕµÊÐ´bS`ӧ]Z̠֚̽eFM| FISH ӽ̰
v8̤ CDC HɉƖȣЪ͓՟̚U|ƔҦʏˢˀSZ9āÕgѬ̲Gz‫̤ײ‬ӑHģ̚U|ϺƆʏ̸ҿĒhƛҮ̸ҿĒaB|ObĤЛbd]Z9
Structural analysis of a pathogenicity chromosome in Alternaria alternata tomato pathotype.
Yasunori Akagi1, Masatoki Taga2, Takashi Tsuge3, Eiji Nanba4, Motoichiro Kodama1(1Fac. Agric., 4Fac. Medicine, Tottori Univ., 2Fac. Sci., Okayama Univ., 3Grad. Sch. Bioagric. Sci., Nagoya Univ.)P-98µ¡ÌůʝŘɿU|Őӄցͯg RTS1 g¹ÀÊ CoRTS1 hÇ‫׌‬οϸϺӑeFC`կŒdҥȆɳʣ8þАƺɳʝɳʣeʇӵaB|
Ʌл ˾, ΛϨƕҍ, Ǐƒ ͤ, àģɦá ‫װ‬õɡǱ֤Ïϥϣ‫ױ‬O}raÇ‫׌‬οϸϺӑeFC`, Őӄցͯg spindle position checkpoint (SPOC) ͓ʣӵѣg 1 ^aB|
BUB2 g¹ÀÊ CoBUB2 H, Őӄցͯbhϴd{ѧҥƥ̠g G1 / S ̠բӧe֛ØU|ObǝƤS`C|9ùƾ, Bub2 gΎʏŴe֛|ÕΏƿȆ RTS1 g¹ÀÊ CoRTS1 eАЉSZ9 ŐӄցͯeFC` Rts1
h SPOC ͜Ҧgý, ѧҥԺőӬ̈gµ¡ÌӽֵġբU|͜Ҧˀ\, rts1 ǫϴ̼hµ¡ÌHկŒeӽֵWVͯѧҥbȃѧҥH҈H]Zϒʝģ^9͋ϏϺƆјϒӑeFC` RTS1 gԎѧd͜Ҧӽ̰hӧ}`CdC9Ç‫׌‬οϸϺӑg corts1 ИǦ̼hǖǎÕeFM|ϥҡ՞ɢ, ҥȆɳʣ։H‫׋‬ӘeΤȬS, țÞŉċȶҦgĎÖОSZ9rZ, ӑјg֫Ǥ֚֫hϕKd{, ӑјļлaֵҪWV҈H]ZҥȆH‫׉מ‬ɢesz}Z9Ò˷, ֵҪSZҥȆgǱպőhֈϥ̼bƛ͔gɳϒОSZH, ÒպeѧҥԺőӬH̢ȏêg҈H]ZҥȆHȉǌSZ9Qze corts1 ИǦ̼e CoRTS1::GFP ӥƙղĈȆȪŀSZbO~, ǫϴɳԺgЋӰHЛԐQ}, ӑј֫ǤeFC` CoRts1 gȳǌHsz}Z9CoRTS1 gˏȒȳǌ͜ҦպčИǦS
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CoRTS1, the homolog of Saccharomyces cerevisiae RTS1 regulating septin dynamics, is required for proper
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Kou Kawabata, Fumi Fukada, Ayumu Sakaguchi, Yasuyuki Kubo
(Graduate School of Life and Environmental Sciences, Kyoto Prefectural Univ.)- 87 —P-99½¦Ì§Çȳǌǒg Enoyl-CoA hydratase hÇ‫׌‬οϸϺӑgϺƆʏϿϝeʇ‫׆‬aB|΅΍ɬđ‫ײ‬Ǯ:Ҿɦ‫ײח‬ȫ̮‫͘ײ҉ח‬ȹѻ‫ײ‬ԦƒʻГ‫ײ‬Ýȹ‫ת‬ҍ‫ײ‬ŀ͹ĢÒ‫̡֋ײ‬ÒȔ‫װ‬ίԴЍǱÏϣ‫ױ‬ҧҠփg'փŴƍʊh‫ײ‬ÒҽЃe½¦Ì§ÇFyk¸ÈÐ¾bCDϴd|ѧҥŉȫƺȑa
ӧ}|ObHДz}`C|9ʤ:h‫ײ‬ATMT ·ϧCZÆÌ ¾жτǫϴ̼gӽ̰Gz‫ײ‬Ç‫׌‬οϸϺӑ‫װ‬Colletotrichum orbiculare‫ױ‬gϺƆʏ͞˗ǫϴ̼ Path-1 ̼ſֵSZ9TAIL PCR ·ey{‫ײ‬Path-1 ̼eFC`hҧҠփ'փŴƍʊgÜʆЃdɷŢʹD enoyl-CoA hydratase Ð§U|ղĈȆHИǦQ}`C|ObӷŐS‫ײ‬OgղĈȆ CoEch1 bƜþMZ9CoEch1 gսœGzh½¦Ì§Çȳǌ¨ÈHӷŐQ}Z9ʤ:hЋƛʏѪ̗Eey{‫ײ‬ˣu` CoEch1 gȏŁdИǦ̼ĕŐS‫ײ‬Xg͜Ҧӽ̰S
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M|'փŴƍʊH͋ϏngϺƆʏϿϝeևӵaB|ObHɰKОƮQ}|9Enoyl-CoA hydratase CoEch1 is essential for pathogenicity of Colletotrichum orbiculare
Kosuke Izumitsu, Yasuka Tatara, Kaori Komatsu, Aya Yokoyama, Takuya Taniguchi, Mami Maruyama, Toshikazu
Irie, Kazumi Suzuki(Sch. of Environmental Science, Univ. of Shiga Pref.)P-100Ç‫׌‬οϸϺӑgˏȒѧҥ͐ʏƿȆ CoTEA4 Fyk CoMOD5 g͜Ҧӽ̰΀Öҍռֆ‫ײ‬Ǐƒ ͤ‫ײ‬àģɦá‫ײ‬Ր ĺ÷ ‫װ‬õɡǱ֤ϥϣ‫ױ‬Ç‫׌‬οϸϺӑeFM|þАƺɳʣgŘɿeh‫÷ײ‬ɈǗԺÕeFM|ֿϥϏЃƿȆúSZѫՂFykțÞ͋Ϗeϩ̫U|ϥϏЃƿȆgƐȚúSZѫՂg 2 ^gϔиSZѫՂg֛ØHОƮQ}`C|9őӬցͯ TEA1 ¹ÀÊ CoKEL2 hŜҗeϐϴЃe֛ØS`C|9őӬցͯeFC` Tea1 hՀǞÌ¯Ժ
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ÊղĈȆ CoTEA4 Fyk CoMOD5 e^C`͜Ҧӽ̰ӧ]Z9cotea4 ИǦ̼h÷ɈǗԺÕaĮպϿӄU|ϴɓþАƺɳʣSZH‫ײ‬țÞ͋ϏÕah͢ɓþАƺɳʣSZ9Ò˷‫ײ‬comod5 ИǦ̼h cotea4 ИǦ̼w cokel2 ИǦ̼bƛ͔eÊ©ÐϥҡewwեɨHԐuz}Zvgg‫ײ‬þАƺɳʣeϴɓhԐuz}dG]Z9āÕy{‫ײ‬CoTEA4 hֿϥϏЃƿȆgƐȚúSZþАƺɳʣgŘɿe֛ØS`C|ObHО
ƮQ}Z9Functional analysis of putative cell polarity factors, CoTEA4 and CoMOD5, from Colletotrichum orbiculare
Midori Kawashimo, Ayumu Sakaguchi, Yasuyuki Kubo, Gento Tsuji(Grad.sch.of Life and Environ. Sci.of Kyoto Prefectural Univ.)- 88 —P-101јϒӑ¿Ê¤È¸¬§ terretonin gϥƙʣe֛|ĥ‫ד‬ցѣҎg͜Ҧӽ̰ȼΣǱՋ‫̮ײ‬ϨĚǱ‫ײ‬ΚɅȊҏ‫֡ײ‬պո̝ ‫̭װ‬Ǳ֤ÏӞ‫ױ‬Aspergillus terreus gϥϦU| terretonin h»Ç §b farnesyl Ǘϩ̫g¤È¸¬ §Gzd|ϐϴd­ ´Ç¢§ǒŴƙϏ‫¿װ‬Ê¤È¸¬ §‫ױ‬aB|9јϒӑϩ̫¿Ê¤È¸¬ §eh͔:dϥϏΎʏ
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Fyk terrenoid bˏȒQ}|ŴƙϏ͌ŐSZ9ϝǌ‫ײ‬O}zŴƙϏg͓՟ͻȒӧDbbve‫ײ‬ýgϥƙʣ֛ՠցѣ Trt6‫װ‬P450‫ײױ‬Trt7‫װ‬non-heme iron-dependent dioxygenase‫ײױ‬Trt14‫װ‬hypothetical protein‫ױ‬ƠtϿϝљ͓ьS‫ײ‬Ƙցѣg͜Ҧӽ̰բu`C|9Functional analysis of the tailoring enzymes involved in the biosynthesis of a fungal meroterpenoid terretonin
Taiki Iwabuchi, Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe(Graduate School of Pharmaceutical Sciences, Univ. of Tokyo)- 89 —§p----------- B -------------Ba Van Vu
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48
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54
̮Ö‫͉װ‬Ϩ‫ױ‬Ўϩҍ 65̮ϨĚǱ 77, 78a, 78b, 89̨̮‫ח‬Ӓ
41ÝȹγÒ 29, 43, 45, 48,
50, 51, 55, 57
Ýȹ‫ת‬ҍ
88----------- j
̭ϨДΌ
Ò̦́Ϙ
ɗֈζ
ɗ֚ΜƗ
ɗ̤Ƭå
ɧλְ÷
ɧϨΌ

-----------63
45
60
36
37, 5430, 7779----------- l
ΛϨƕҍ
УƆ˨ӧ
У̤ȋ
ӟðų
ӟðƀ
ӟðҏà
ӟȴϢɱ 47
ӟȻ̏Ś
ӟ̡ҙɗ
ӟ͉˱Ƴ
ӟƆӯȆ
íСΊǗ
į́Ƌƕ

----------87
85
41
80
42
41

- 91 —

44
51
36
41
52, 64, 6876----------- s
ÔΒʛ
ÔΒйɗ
ʹֈ͢Γ
ʹֈđ‫ח‬
ʹԦ΂
Ô͛֩к
žэʉ
ÔƆҜ
ȖɅ‫÷ך‬
Ṳ̑ĭǳչ----------48
34, 4232, 6656
32, 407747
34, 426773----------- t̨ǓŃӆ----------41, 67----------- v ----------ĺ̮ծ64̤ȹ‫מ‬ə
76a, 76b, 79
͉Ò͚
68
͉ź̂
59
͉ԸÒչ
36
͉ϨȠ÷
50
͉ȹӯĻ
83, 85----------- w ----------ГֈʣƩ
47
łƆҍ;
83
ȹɳΌɗ 50, 61, 62, 63,
65a, 65b, 66, 67
ȹɅѬ
56
ȹϨְ÷
33, 62
ȹϨöР
43
ȹ̤йå
59
ȹ̤Ўɪ
42

͍ȫ
----------- x
զéҒǳ
ΨԦ̏
ȯʋ֍

74
----------71
82, 8476----------- y ----------ϧáÝƬå
81
͘ȹѻ
88
ƚ̻ĢФ
43
ƚϨԔ
39
ƚӷƳ
39, 44, 46, 47----------- 
Ӆð̐
Ҩ̤˨ə
ƩϨ͢ǳչ
ƩϨ̛Ȇ
ΥնñåȆ
ΥՏԸí
Υշɀ÷
ΥՏ֩Ɨ

----------43
74
68
41
70
30
36
36

- 92 —јϒӑőȆϥϏȋЗгć ćŚ
1.

本会を糸状菌分子生物学研究会（Fungal Molecular Biology Society of Japan）と呼ぶ。また本会が開く研究会を糸状菌分子生物学コンファレンス（Conference on Fungal Genetics and Molecular
Biology）と呼ぶ。

2.

本会は糸状菌の分子生物学、細胞生物学、生化学、生理学、遺伝学などの普及発展を目的とする。

3.

本会はその目的を達成するために次の事業を行う。
2.

研究会及び総会の開催。

3.

会報の発行。

4.

関連研究団体との協力事業。

5.

その他、必要と思われる事業。

4.

本会はその目的に賛同して入会した個人会員及び総会において承認された名誉会員を持って構成する。

5.

本会入会希望者は所定の入会申込書を提出し、別に定める入会金を納入するものとする。

6.

本会はその運営のため、会長、運営委員若干名および会計監査 1〜2 名をおく。任期は 2 年とし、改選は運営委員の推薦と総会の承認による。
(1)

会長は本会を代表し、会務を統括する。

(2)

運営委員は運営委員会を構成し会務を審議する。運営委員には庶務、会計、編集担当、広報担当
をおく。

(3)

本会の会計事務局は会長が指名する会計担当の運営委員の所属する施設に置く。

(4)

会計監査は本会の会計を監査する。

7.

本会は事業運営に必要な実費を年会費として個人会員から徴収する。

8.

本会の事務年度は 7 月 1 日から 6 月 30 日までとする。

9.

前事務年度の庶務、会計については、これを総会において報告し、承認を得るものとする。

10. 本会則の改定には総会出席者過半数の賛成を必要とする。
以上
補則
(1)

本会則は 2001 年 7 月 1 日より発効する。

(2)

本会入会金は 1,000 円とする。

(3)

年会費は一般会員 2,000 円、学生会員 1,000 円とする。

(4)

研究会の通知及び会報は、当該年度までの会費を納入した会員に送付する。

(5)

2 年度にわたって会費納入のない会員は、その資格を失うものとする。

(6)

研究会の発表者は、会員に限るものとする。新入会員の演題申し込みは会費納入の確認を持って受理する。

（平成 23 年 11 月 16 日改正）

- 93 —¢NghG®YfXGW¹2013 o 6 } 30 {ºć  ֗ïƦ Ųå

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̭õǱȋǱȋ֤ՎȋϥƨЧȋЗгЧïŹɃǍ˻Ḙ̑õǱȋǱȋ֤ՎȋϥƨЧȋЗгЧȱ֛ ĭí ֊ͿɈ͏Ǳȋ®

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̭õՎɈǱȋǱȋ֤ՎȋЗг֤ȹϨ ĥ 


ćԀІ̺

нŉ ժ֯

ϔиӧ˦·÷տ‫׌‬ѼƙЗгʩÏŴȋպ

¾Á¯Ì̼ɩćПЗг֙Ͽպ̭õՎɈǱȋǱȋ֤ՎȋЗг֤- 94 —¢NghG®YfXGW¹º¹2013 o 7 } 1 {! » ºć  ֗
小林 哲夫

名古屋大学大学院 生命農学研究科

էƷȀƫ֡պ ˭ʕ‫װ‬ćԀʹɲ‫ױ‬̭ŵǱȋǱȋ֤ՎȋЗгЧ̚Ȼ ȋ‫װ‬ɥűʹɲ‫ױ‬̭õǱȋǱȋ֤ՎȋϥƨЧȋЗгЧïŹɃ Ǎ˻Ḙ̑õǱȋǱȋ֤ՎȋϥƨЧȋЗгЧȫоƆ Ρ֗ȻʮӨЧȋǱȋϥϏљŧӟ ְǨ‫װ‬ćԀÏҁֱʹɲ‫ ױ‬ƜǔǱȋՎȋպɅƒ ŝƗ‫װ‬ɜǝʹɲ‫ױ‬Ǳ֞ɡиǱȋǱȋ֤ϥƨϣǢЧȋЗгЧĐֈ ĺ̇

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