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発表演題および講演時間
特別講演
11 月 16 日（水）16:45 - 17:45「酵母から見えて来たオートファジーの分子機構とその生理機能」
   ­S±y ÙlÏÒø 9GH2ÏÒ³²  yùá_シンポジウム11 月 17 日（木） 9:25 ‒ 12:00「糸状菌・キノコの形態形成の意義とその分子機構」
9:25- 9:30
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9:30-10:00
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10:00-10:30
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10:30-11:00
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11:00-11:30
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11:30-12:00
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12:00

O-1

լѺ A. oryzae ~ MAP ¢¾å± AoFus3 yϓăĥγlˍҡ´âÅ¤Ӕ~̴њҧ̅ϚѽǫӏնõȬΉçնÖzgür Bayram1նOliver Valerius1նGerhard H. Braus1նƃ˷žfմ̂ǫԲäӧαϯäɼαȾն1 §¸ºâ§âǫȀյ

12:12

O-2

Aspergillus nidulans Ł NO ÞwŀĐœŐŢŮşƂź P450 Ł â ĽķŁm .
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ğĜÈağğcğ3­ąğ_ė[ğ¶µUğěõÀƆÐ¥SĒƄ³?²MƇ
12:24

O-3

Aspergillus nidulans |]dեͺ˿Ĕìx~Ëàºâ¢¾å± C |Ñ¼å¬®ҵȞʜū̴̫~ҧ̅ΡȬήýնǙŚҘù, ǭδ˘ɵ մ̂ǫԲäӧαϯäɼαȾյ

12:36

O-4

МΥѺ Aspergillus nidulans |]d<-1,3-¥ÞâƣʒԒУԄĘǼ agsB Ϡǥ̓] agsA, agsB ĂԘϠǥ̓~̴њҧ̅϶ѿ̞ ƤҠƻ ԯԌ˅ʇ  մ ̂ƃǫԲӧäαΣβ̧Ŵʒ ̂ƃǫä˵̀ϟյ
12:48

O-5

Ť͛ǔÜ¸

å±(( !- ʜū̓|]d¬´¦ЦјǤ̫ӵ~ǧƂôţƻǼնǒ˷Ҙç մȯʘαȾϟյ

13:00

O-6

΢ѶŕαМΥѺ Epichloë festucae ~ĞŤǼԚ G ´âÅ¤Ӕ Cdc42 ƕ RacA ~̴њŤƂ|ԫlϟϹ
̩ԙƖ՟նЂ˷ǫƬմƦǫԲäαƱӧȀյ

13:12

O-7

ѺՖ|]dÆ®¼âԄĘǼŚâ¼àâ~ӷƂҝδ͢ɂնȢ ɽԞ մ̂ǫԲäӧȀαƱϯȀϟϹϯյ

13:24

O-8

լѺ A. oryzae ~Ѻ̔ɦʒıӷǆǼ SclR ~ӽŲψΫ̓γZqѺМҌƣњ~ҧ̅Ʋδձǭ, ԛ ԟŀ 1, ȟȬ͠Ă 1, õȬΉç, ƃ˷žf(̂ǫԲäӧαϯäɼαȾ, 1 ԙδβϟ)

13:36

O-9

լѺ|]d HECT ÙÇ¢¶âÝ¡å± (hulA) ˿ĔϋψΫ̓~ĥʒδôίɁնˍӂȡɠնĄƯžý մ̂ƃǫԲӧäαΣβ̧Ŵʒյ

13:48

O-10

լѺӞŞǆǼ XlnR ~ҵȞΣӔĬǾϋÝâԓƂyo~αέȀϋʌъϝȺƮɌ, ԙƜϨơ, ԛõĉǼ, ŷ҇ԿǦպ, ȟ̇ƶǮմƦǫԲäαƱӧ, պƦǕǫäӧյ

14:00

O-11

Trichoderma reesei ~°ÞÜå±αβ|ԫîl¬¥¾ÞĘӿԫӶ´âÅ¤Ӕ~ʯФ˒ìϭҎնƝȺԶО, ͰӂѲƿ, ɻδ͢čնȟЄƐ ͷ մԧȮʚϯǫäαΣյ

14:12

O-12

϶ZsςѺ Magnaporthe grisea |]d̤Σ»Éâ¬â~ĥγÕ¿¯Ôҧ̅ȟկѮƟPȬȶˤéP̟˷̺ùPե˴̺ӾPեø͢˧մˍΈ҆ǫäɼαϯյ
14:24

O-13

ÁZsςѺɢ͇ƂÒ¨Þ® MoCV1 ~ɿΥҧ̅ƕÞ®´âÅ¤Ӕ~̴њҧ̅ǭδ˦ǼնͧȬĲçնϬƐˁҎնˮ͏ŵնȗȮɷն̢ȬҘōմ̂ĉӧȾǫԲäӧյ

14:36

O-14

ÝՖΓρςѺ~ςƐɿ|ɹՐxYŤ͛´âÅ¤ӔԄĘǼ LAC2 ~ƥȉƕ̴њҧ̅̇ Шĕնǳδ̉Ϸնǳԙƶԋնեԙъǿ մĉǫԲäӧյ

14:48

O-15

Cryphonectria parasitica RAS3 ´âÅ¤Ӕ~αƂȀäαέȀϋɿӔȬŚŊӤPե̳ʥýPЄƐЧ մȌǕǫä՘β̧äΰǢյ

4

Kàé¹ăO-16O-26Ą 11 © 17 ¦ăªĄ 14:30 – 16:42
14:30

O-16

A. oryzae ^~íͥɿǔĂ̷đҾ¤Ü®´åͥɿƂyαƣʒβΣ~̫ӵҧ̅ô͕ǹĊնϝŚżçԋն͹ӨӓĂ մՉȮϕǫä҆յ

14:42

O-17

®¤àå® Fusarium asiaticum ~¼Ý¨º°âН͇УβαҵȞlȺë ՕP*ôȴ ԶPɌœԘçù մΦ͜ü͓ӧϟä*՘ƳȄŒǸƵėյ

14:54

O-18

laeA ξϵψΫ| Cordyceps militaris ~Ă̷đҾͥɿƂ˴ì ͨ 1նRina Rachmawati1նąƐƟԾ 2նċɌƉý 1
մ1 ԬǫäαΣȾȀǌԻćͦ°ն2 ӧϟ̴̫ä̲̈ϟյ

15:06

O-19

ԄĘǼǪԘϠǥ| Aspergillus oryzae ~ Hydrophobin щ~̴њҧ̅ȬȺ Ьն͋ԙġ՟նôȴ˛Хմ˘͘ǫäӧäӧƂյ

15:18

O-20

͋˥ʪŻǼÒ¤àÄÜâ® (QCM) |ιՋͥɿҊϊӔ RolA yǋĢғՋԪ~
ϓăĥγҧ





δԈɠͅ նǫՖˣǼ նëƐĽĂ նե̳ɷ նŠ̱ӏʒնˮδϴɧնԯԌ˅ʇ մ ̂ƃǫԲäαΣβ̧Ŵʒն ԐՖϟäǘϏն̂ƃǫä˵̀ϟն̂ƃǫäǪŌϟյ

15:30

O-21

МΥѺ Trichoderma reesei ~°ÞÜå±եαβǧξ̓~ɦʏȀϋҧ̅ɻδ͢čպնˍδшӏǼպPǫԵ̺ǼջPȟЄƐ ͷպմպԧȮʚϯǫäαΣPջеƣθňϟϹʽʶյ

15:42

O-22

Aspergillus nidulans ~ɦӔӞʴ˞|ՊϓƥˌΡʹŷlfy|´å§ºâ¥~ŹΪƂĖ҇ՈǮ 1նԛйǲ 2 մĴȻǫä1 ŒȀ˂і̴̫ն2 έȀԌ Ϋր̂ǫԲäŎЀαƱյ

15:54

O-23

МΥѺ|]dԄĘǼ̴њҧ̅~q~ŤǼαΣȀϋ¹åÞ~ԩψƐȴĲ˘նҝ˼խԗ͏մαΣϟյ

16:06

O-24

ȊγլѺ̓~§ÂÔӷƂyƢԖӵβ̧~ԂɼĖ҇ Ȱ 1,2նǶȣʆɌ 1,2ն΋˼ʁǼ 2նɵȮ˗ˉ 2նϣӂ˄Ǽ 2նȬδ ĵ 2նȯìƲҘ 1, 2մ1 ɑȴǫԲäŎЀϟն2 Ԑкϟյ

16:18

O-25

Ò´¦Ö»Þyjq¢Â¨αі̴̫ҧ˘~§ÂÓ¤®Ëàå¶Ķ̳˄ 1նĠ҇ϖù 1, 2 ,3նҝǙёê 1ն̢҇ˉƻ 2, 3( 1 ՄǌZqdն2 ÃÉ­Á¬®ն3 ̂ĉȎˀǫ )

16:30

O-26

ǑҒՖ~ŕαѺ~§ÂÔҧ̅ƐŏĂԋնĠ҇^նȟȳ̺ƟնȬ˷ǴƲ մϮδϕǫäαΣӒͽյ511 © 16 ¦ă¶Ą15:10 - 16:40ăz¤ÈkĄ
11 © 17 ¦ăªĄ13:00 - 14:30ă\¤ÈkĄ<-0JËã
P-1
Aspergillus awamori ~ ligD ԄĘǼϠǥ|եՒɗϓƥЪʴ\Ȑö~ԩψե̳ɷ 1, ͋ӂ͘ 1, ϊϝ͢Ɍ 2, Ȭδĵ 1մ1 Ԑкϟ, 2 Ç¸¤յP-2Cre-loxP ¬®ºÔγZqլѺ|]dǪԘԄĘǼȞő¬®ºÔ~ԩψ͏Ɛϒ̲ն͋ӂ͘*նˍӂȡɠնĄƯžýմ̂ƃǫԲӧä*ԐкϟյP-3϶~ԒУКƂɿıӷlϊѬў˹ѺÃ´¦¶×µ©¦|]dԄĘǼȞőН̫АȬȱӓ͘ 1ն˴˼Ĳù 1ն͹ӨԶơ 2մ1 ӧϟ̴̫ä̂ƃӧϟP2 ĉǫäαǾϟյP-4МΥѺ Coleophoma empetri F-11899 |]dջϵ~̷ðđ¬å¦âªåγZq§ÂÔ°âÊÞԎŦ~͈ӡôӂƲýնȬδԿĊնфԙԴčնϣӆ͠ɠն̳˷̺͘ մȓȬϕǫäαΣȾյP-5МΥѺ¼Üâ®¤ÝË¼åÔԎŦ»å´γZq§ÂÔÂºå¬Úâմ̂ǫԲäӧαϯյĄƆȸǐ˒ǼնǙƇ˘նէȴ̺ͨնմ­¾Ý®յë˼͠ǯնЂδй

P-6ʞѺͥɿˮlĆΟɇɿɴαΣ~§ÂÔϯȀϋҧ̅ʕδ˦шնȟȬѮŘ,̝˼ѩǼ, ȟ͐Ѳ˘, ηδԿùմβ̧ʚҏкƣϟϹʖäαΣËà°®յ
P-7

ϊլѺ Aspergillus kawachii IFO 4308 ̓~§ÂÔҧ̅ĂϪ͠ǘ 1ն̢ç̲ 1նȬìɨǨ 1նƲδ̺ǭԋ 2ն̠Ɛɚƌ 2նեìϭ˛ 2նǫ̢Ĳԋ 2ն
ЂȺ҅ 1նδđɚč 1նøƐƶ 1նɮ̺҇Ū 1 մ1 üǫԲäӧն2 êƲԐՖյ

P-8

SOLiD3 |լѺ~ RNA-seq yլѺ͈ӡ§ÂÔ»å´Íå®~˪ˍȯìƲҘ 1նȴƐ˘Ǽ 1, ë˼͠ǯ 2, Ȭδ ĵ 1 մ1 Ԑкϟ, 2 ̓ɞėϧ­¾Ý®յP-9Ü¤µὲç˷ԥʞĢƞǧԌĝ VHH ~լѺ Aspergillus oryzae |Ť͛αβՇ˴ͱç 1նδͲѓçԋ 1նȮղˉш 2նѸԙƇϮ 1նδô ź 2նøδƌŌ 3նϰ ͢Ă 3ն
Ӫ҇ ˝ɧ 1
մ1 ϪʕǫԲäȾäɼƂն2 ϪʕǫäŎЀҌƣϟϹΰն3 ˭̘şäкϟյ

P-10

լѺ A. oryzae |]då¼É­åūɱ|ξϵ´âÅ¤Ӕαβɿ~ƧëѹԪԶɻ 1նȢ Ӣȃ 2նõȬΉç 1նƃ˷žf 1 մ1 ̂ǫԲäӧαϯն2 ƻ˘ǫȀ̒ä҆յ

P-11

լѺ A. oryzae ὲ¢¬Ü¾å±~ичϋ¤àå¿â¥ yψΫαβøδƌŌ 1ն͞ԌʇǼ 1նǡδƲŶ 1նǒ̂ɠ̲ 1նǛͨǼ 1նϝδƌ̲ 1նϰ͢Ă 1ն
Ӫ҇˝ɧ 2ն̤δōш 3 մ1 ˭̘şäкϟն2 ϪʕǫԲäȾäɼƂն3 ĉǫԲäӧäɼγαƱյP-12¤âԓαβМΥѺ Aspergillus niger |]d oxaloacetate hydrolase ԄĘǼ(oahA)~ϠǥyեψΫ|¬ØԓαβЫӚ~̥үȟ̇ʐçն˷δҘ̲ն˰ԌӏΊն̚˼ŏǭԋ մ˔ǫäέȾäɼƂյ

P-13»âËâӔԳƒȟժÉ®ÒγZqլѺψԒx~ƞͿƂ|ԫlϟϹԜ˴˟ նƲδϖĊ նô˼ơ նĠԙŌ˝ նȣԫĽĂ նǫЍĴç նԛǼ˘Ҙ   ԛ͕Ⱦǫä§ÂÔϟ˒͵ÉÞÒ

P-14ͭĢǗǑxեαβlլѺ̴њ˵ϛ´âÅ¤Ӕմ .&յԄĘǼ~ҧ̅yŪγϝȶͨϿնИĶ͠ӣնʘƚȨ̙ǼնĠԙŌ˝նȣԫĽĂնǫЍĴç մԛ͕Ⱦǫä§ÂÔϟյ6ȊγլѺ̓~§ÂÔӷƂyƢԖӵβ̧~Ԃɼ

P-15Ė҇ Ȱ 1,2նǶȣʆɌ 1,2ն΋˼ʁǼ 2նɵȮ˗ˉ 2նϣӂ˄Ǽ 2նȬδ ĵ 2նȯìƲҘ 1, 2 մ1 ɑȴǫԲäŎЀϟն2 ԐкϟյМΥѺՖxɑbĳǾhq̴њ˵ϛԄĘǼ~լѺx~ҧ̅P-16 ŠȺƟǼ ąõ ϒ ȗʕɻĳ ͐δŊέǼ ɮ̺҇ŪȯìƲҘ   





P-17

մ ɑȴǫԲäŎЀϟ Ԑкϟ üǫԲӧյДիեαβ´âÅ¤ӔԄĘǼϠǥ̓~Ԗӵë~Τɿ|uZwѭδΛ˘ 1,2նϬƐϖçԋ 1,2նì˷ՏǼ 2ն͖ԙш÷ԗ 2նȯìƲҘ 1,2նȬδĵ 2
(1 ɑȴǫԲäŎЀϟն2 Ԑкϟ)

P-18

ÌÔƣʒԒУ porphobilinogen deaminase  Aspergillus nidulans  nitrosative stress ^ĳӁlƮžˁնթϪș˝, Ҏ˴ɡԜ, ë˼˩č, ˚ԙӏҎ, եӂϒ̲մЈ͞ǫԲäαƱΰǢյP-19̶ǰǧξĢγZqլѺ AoSO ´âÅ¤Ӕ~®¼ß®ɼЉɿšՀ̴̫~ҧ̅Ġę ǐ, Cristopher Sarazar ESCAÑO, õȬΉç, ƃ˷žfմ̂ǫԲäӧαϯäɼαȾյP-20Aspergillus nidulans |]d ypdA ԄĘǼψΫūɱ̓~ҧ̅лȺҘѫ նѽƐǫϨ նƤҠƻ նԯԌ˅ʇ  մ ̂ƃǫԲäαΣβ̧Ŵʒ ̂ƃǫä˵̀ϟΫƇѿǫäϖѺƅյ

P-21МΥѺ Aspergillus nidulans ~Æ®¶­â¢¾å± PhkA,PhkB ~̴њҧ̅ĠƝϛшȬȶ^ԛõĉǼŷ҇ԿǦ ȟ̇ƶǮմƦǫԲäαƱӧƦǕǫäӧ յ

P-22

լѺ A. oryzae ~ MAP ¢¾å± AoFus3 yϓăĥγlˍҡ´âÅ¤Ӕ~̴њҧ̅ϚѽǫӏնõȬΉçնÖzgür Bayram1նOliver Valerius1նGerhard H. Braus1նƃ˷žfմ̂ǫԲäӧαϯäɼαȾն1 §¸ºâ§âǫȀյP-23ςƐϖѺ Aspergillus fumigatus |]dͫӱǎɼЉЫӚ~̴њҧ̅ѽƐǫϨնĄÂąӱնȺ˷ӷմƇѿǫäϖѺ°â´åյP-24

Neurospora crassa ~ MAK-1 ƕ MAK-2 MAP ¢¾å±~Ϡǥ̓~ғΫǔyȥǏҧ̅þąҶù, ե̳̺Ʋ, çϝ˝ɧ, ҇˼ϖմ̂͢ǫԲäαƱϯȀյP-25Aspergillus nidulans |]dեͺ˿Ĕìx~Ëàºâ¢¾å± C |Ñ¼å¬®ҵȞʜū̴̫~ҧ̅ΡȬήýնǙŚҘù, ǭδ˘ɵ մ̂ǫԲäӧαϯäɼαȾյP-26

լѺ Aspergillus oryzae |]dɴȟЌɦʒôɸԫӶ´âÅ¤Ӕ AoApsB ~̴њҧ̅ȺκЯɌPϚѽǫӏPõȬΉçPƃ˷žfմ̂ǫԲäӧαϯäɼαȾյP-27լѺ A. oryzae ~ʧuЦјӔǔÏ®ÏÝÅå± A2 ̬´âÅ¤Ӕ AoPlaA ~̴њҧ̅բąОùնȟӂ˗Ɍնƃ˷žfնˮȮ Ȁ մ̂ǫԲäӧαϯäɼαȾյP-28լѺ A. oryzae |]d Ca2+ՊĬǾɿÏ®ÏÝÅå± A2 ̬´âÅ¤Ӕ iPlaA ~̴њҧ̅ȟ̳Ɯѓնƃ˷žfնˮȮ Ȁ մ̂ǫԲäӧαƂäɼαȾյP-29

լѺ A. oryzae |]dâ½ª¼å¬®ԫӶǆǼ AipCնAipD ~ȥǏ]̴њҧ̅̃ȣӓĊն̭ƜҘ̷ԋնˮȮ Ȁնƃ˷žf մ̂ǫԲäӧαϯäɼαȾյ

7

P-30

լѺÒÞ¼å®ÅåÓå±~ƢϵΓУͽ|ЦјŚȥǏǧƂ
Ɍ˷ƶýնǫӾƜͮǼնδôίɁնˍӂȡɠնĄƯžý(̂ƃǫԲäӧäαΣβ̧Ŵʒ)P-31МΥѺ Aspergillus nidulans |]d<-1,3-¥ÞâƣʒԒУԄĘǼ agsB Ϡǥ̓] agsA, agsB ĂԘϠǥ̓~̴њҧ̅϶ѿ̞ 1, ƤҠƻ 2, ԯԌ˅ʇ 1,2մ1 ̂ƃǫԲӧäαΣβ̧Ŵʒ, 2 ̂ƃǫä˵̀ϟյ
P-32

Aspergillus nidulans ~¢¶âƣʒԒУ CsmA ~ѺМŚȥǏƂ|]d¢Á¬â~ɫųȝȶϖ̦PǙŚҘùPǭδ˘ɵմ̂ǫԲäӧαϯäɼαȾյP-33МΥѺ Aspergillus nidulans |]d Saccharomyces cerevisiae CRH Þ²à¥~̴њҧ̅ϣ˼Ɏ͘նȝȶϖ̦նǙŚҘùնǭδ˘ɵ մ̂ĉǫäӧäαƂȾյP-34МΥѺ Aspergillus nidulans |]dţѰԒ͆ Saccharomyces cerevisiae SKT5
Þ²à¥ AN3445 ~̴њҧ̅˚ ͨѣնȝȶϖ̦նǙŚҘùնǭδ˘ɵմ̂ǫԲäӧαϯäɼαȾյP-35լѺ A. oryzae |]dԃʡϋå¼É­åԫӶԄĘǼ Aoatg11 ~ҧ̅δʖԶùնѹԪԶɻնƃ˷žf մ̂ǫԲäӧαϯäɼαȾյP-36

Magnaporthe oryzae ~ŤƂ̬ɞyå¼É­å̴̫|ΣӔđҾ~ԫӶɿąëŷǲǼ 1) 3)նϑկСǼ 1)նř̃ѓǼ 2) 3)ն˸˽ŋ 1) 3)ն͐δĽç 1) 3)
մϪʕǫԲ 1)ä̲̈ϟ 2յäαϟ°â´å3)յ

P-37ʤǼѺ¬¥²Æ¼Û´¦|]då¼É­åԫӶ´âÅ¤Ӕ CcAtg8 ~ҧ̅͹ԇ ɩնɡαӏҘնƤ˼˗ɵնխδʂɧմ՟Ⱥǫäӧäɼαϯյ

P-38

ʤǼѺ Coprinopsis cinerea ~´âÅ¤ӔŤ͛~Ó¬â~ԫî̳˷ɑϨ 1ն̰δʇԾ 2, ˍŐӤζ 2, ƤδҶ 1 մ1 ̂ĉӧȾǫäӧ, 2 ŖəϕǫäαƱέյP-39ʤǼѺ¬¥²Æ¼Û´¦~Łʒԧ|ԫԄĘǼ cag1 ~ҧ̅˼Ɯ ŌնΚȬƲ̲ մϮδϕϾǫäαΣӒͽյP-40΢ѶŕαМΥѺ Epichloë festucae ~ĞŤǼԚ G ´âÅ¤Ӕ Cdc42 ƕ RacA ~̴њŤƂ|ԫlϟϹ̩ԙƖ՟նЂ˷ǫƬմƦǫԲäαƱӧȀյP-41ƯǁγĞÏ®É´å±լѺ~ДլնӃլ|]dÏ®É´å±ԄĘǼψΫõąͱç˱ 1նǪδŶα 1նϬȮϖԗ 1նԜ˴ ѓ 1ն˰ԌՑǭ 1նƲø ӄ 2նϊϝ͢Ɍ 2նƃ˷ŭҎ 3ն˺˷ӿƴ 4ն̦˷ʑç 1
մ1 ӧϟ̴̫ä՘кϟն2 Ç¸¤ն3 ʍϛβʚϟä՘Ⱦʚ°ն4 ¾

P-42

Öյ

ƯǁγլѺ A. oryzae KBN630 ̓|]dԓɿÏ®É´å±մAphյԄĘǼщ~ϠǥȄδմƤԙյɒǼնԧӂȺʸնȟԙǲͤǼնĖӑĦш 1նϊϝ͢Ɍ 1նƲø ӄ 1ն˺˷ӿƴ 2ն̦˷ʑç 3նƃ˷ŭҎմʍϛβʚϟä՘Ⱦʚ°ն1 æÇ¸¤ն2 ¾

P-43

Öæն3 ՘кϟյԄĘǼǪԘϠǥ| Aspergillus oryzae ~ Hydrophobin щ~̴њҧ̅ȬȺ Ьն͋ԙġ՟նôȴ˛Хմ˘͘ǫäӧäӧƂյP-44͋˥ʪŻǼÒ¤àÄÜâ® (QCM) |ιՋͥɿҊϊӔ RolA yǋĢғՋԪ~ϓăĥγҧδԈɠͅ նǫՖˣǼ նëƐĽĂ նե̳ɷ նŠ̱ӏʒնˮδϴɧնԯԌ˅ʇ մ ̂ƃǫԲäαΣβ̧Ŵʒն ԐՖϟäǘϏն̂ƃǫä˵̀ϟն̂ƃǫäǪŌϟյ

8

P-45

Â¢´¦ὲ~ hydrophobinմFv-hyd3յҌƣ´âÅ¤Ӕ~αβƤδϖΊ 1P҇δțɎ 1PҝȺѫɌ 1P϶Š ѓ 2PδƜʅ˱ 1Pìǒ Ҷ 1
մ1 ĴȻǫäтжäɼαНP2 Ï¤¼`~fкƣϟϹʖյ

P-46

ծլѺ KexA ~̴њҧ̅Šδ͔ђǼն̢δȕĊնŮδͨնЂŚӾԾնȬɦ͢Ɍմ̂ӧȾǫԲäɼαϯյ

P-47

լѺ serine-type carboxypeptidase ~ Aspergillus nidulans |]dեψΫ̓~̥ҫĠԙƲОն̢δȕĊնŮδͨնȬɦ͢ɌնЂŚӾԾ մ̂ӧȾǫäɼαƂյP-48լѺὲˍҡ β?¥Þ¨¬µå±|Τɶϋ{ǘӔΤξɿȾ҇ĦԉǼն͉ȎʒԶն͹Ԍ˝նˍӂȡɠնĄƯžýմ̂ƃǫԲӧäαΣβ̧ŴʒյP-49Ъʴ\լѺαβ°ÞÜå±γZqɑѿ̲¤ÜÉ¼Å ÞË~ԒУКƂƤ̑Ĳϭ 1, Ԑąыơ 1, Ȯղˉш 2, ѸԙƇϮ 1, δô ź 2, øδƌŌ 3, ѽƐěƴ 4,ϰ ͢Ă 3,Ӫ҇˝ɧ 1
մ1 ϪʕǫԲäȾäɼƂն2 ϪʕǫäŎЀҌƣϟϹΰն3 ˭̘şäкϟն4 ˒˷ТÅÞËϟϹʖյ

P-50

ʤǼѺ Phanerochaete chrysosporium _âÖ¿Ţέ˴˻~Ťҧ˞|αβlԒУ~°¤ß¼åÔҧ̵̅˴Ά ǙƇ˘ ̇ϦǼ ê̳ϭç ĄƆȸǐ˒Ǽ էȴ̺ͨ մ ̂ǫԲӧαϯP Ä´ÂåÞՌˍʚҏϟϹЪƣյ

P-51

КӔđҾԫӶԒУԄĘǼŪγjq˴˻ў˹Ѻ~НбŤՖƲδ˯ǼնĄƆȸǐ˒Ǽնէȴ̺ͨ մ̂ǫԲäӧαϯյ

P-52

լѺ A. oryzae ~Ѻ̔ɦʒıӷǆǼ SclR ~ӽŲψΫ̓γZqѺМҌƣњ~ҧ̅Ʋδձǭ, ԛ ԟŀ 1, ȟȬ͠Ă 1, õȬΉç, ƃ˷žf (̂ǫԲäӧαϯäɼαȾ,1 ԙδβϟ)

P-53

Aspergillus nidulans ~ NmrA y AreB |Õ¾­âѐɿƂ̴̫~ҧ˘Ė҇ѲԗǼն̜ȣĲč, ɻ͋ŌĈ, եӂϒ̲ մЈ͞ǫȀäαƱΰǢյP-54Aspergillus oryzae ~®¼ß®˿Ĕìx~αіҺЎ|ԫӞŞǆǼ~ҧ̅կδ˘Řնեӂϒ̲մЈ͞ǫԲäαƱΰǢյ

P-55Îß¿ÞÜ¥Ü®ŕαѺ Epichloë festucae ~ ProA _ЬƣlËàÖå´åԎŦ~ƥȉδôʍǼ 1նSanjay Saikia2նGemma Cartwright2նЂ˷ǫƬ 1նŷ҇ԿǦ 3ն̤̎ȡɻ 1նBarry
Scott2մ1 ƦǫԲäαӧն2Massey Univ.ն3 ƦǕǫäӧյ

P-56

Aspergillus nidulans AmyR ~ҵȞΣӔĬǾϋ{Ťҧ|ԫlҧ̅
ǫȶԴǯ,

P-57

̢˷ыǭ,

ԛõĉǼPŷ҇ԿǦPȟ̇ƶǮ մƦǫԲäαƱӧȀյAspergillus aculeatus ace1 ̶ʷ̓|]dКӔŷ͋ŤҧԒУ~αβyǡ®¼ß®ɼЉ~ҧ̅ȬղʍɡնȟҝȆƲնӂĵ͘նΓӂՏçնȺƜůơ մǫԬɖϾǫäαΰϯյ

P-58

Aspergillus aculeatus Zn(II)2Cys6 ǔӞŞǆǼ CGAF °Þàå®ɿǘӔ|ɼЉjw°ÞÜå±äÌÓ°ÞÜå±ԄĘǼψΫͥɿƂlǍ̻вшնӂĵ͘նΓӂՏçնȺƜůơ մԬɖǫԲäαΰϯյP-59МΥѺ|]d°ÞÜå±αβūɱ̴̫ՇȬ˵̀նԛõĉǼնȟ̇ƶǮ մƦǫԲαƱӧäαΣ̴̫յP-60լѺӞŞǆǼ XlnR ~ҵȞΣӔĬǾϋÝâԓƂyo~αέȀϋʌъϝȺƮɌ, ԙƜϨơ, ԛõĉǼ, ŷ҇ԿǦպ, ȟ̇ƶǮմƦǫԲäαƱӧ, պƦǕǫäӧյ

9

P-61

Trichoderma reesei ~°ÞÜå±αβ|ԫîl¬¥¾ÞĘӿԫӶ´âÅ¤Ӕ~ʯФ˒ìϭҎնƝȺԶО, ͰӂѲƿ, ɻδ͢čնȟЄƐ ͷ մԧȮʚϯǫäαΣյP-62МΥѺ Trichoderma reesei |]dˍҡҵȞψΫ̴̫~ʯФĠ҇˛ѻնՄϖɠնȟЄƐͷ մԧȮʚϯǫäαΣյ

P-63

Trichoderma reesei ~͈ӡ§ÂÔҧ̅|ǘvbˍҡӞŞǆǼ ClbR ~̴њҧ̅ˍδшӏǼ 1,2, ȬƜ՟ф 1, ɻδ͢č 1, ̢ç̲ 3, ɌȺϭ̲ 4, øƐƶ 3, ̢Ⱥɚ 1, ȟЄƐͷ 1
մ1 ԧȮʚϯǫäαΣ, 2 JST, 3 üǫäαΣӒͽ, 4 ^mh DNA ϟϹʖյP-64լѺËàºå± AOEXE103 ~Þ´¾ºÊ®ËÜ¬â¥øĳȴʄ̢δȕĊŮδͨȮ˷йǼȬɦ͢ɌЂŚӾԾմ̂ĉӧȾǫԲäӧäɼαƂյ

P-65

լѺ°Ýâ´ËÞÐ¢¬ÎË¶µå±ԄĘǼщ~ичϋψΫҧ̅ԯĳ˛ѭն̢δȕĊPȮ˷йǼPŮδͨPȬɦ͢ɌPЂŚӾԾմ̂ӧȾǫԲäɼαƂյP-66҆Űʭţ ABC ¼Üâ®Ñå´åūɱlӞŞǆǼ AtrR yϓăĥγlǆǼ~ʯФǫǞ̼նδôίɁնˍӂȡɠնĄƯžýմ̂ƃǫԲӧäαΣβ̧Ŵʒյ

P-67

A. nidulans ~ AmyR ¥Þ¨å®ǾǏì~®ºÝ¥Ò¼¬® ¶â~αƣʒʜūlë˼˩čնթϪș˝նɻ͋ŌĈն̜ȣĲčնեӂϒ̲ մЈ͞ǫԲäαƱΰǢյP-68լѺ|]d¨­ԓαβԄĘǼ~ҧ̅ĠԙŌ˝ǚ˷ŏǼȟ͐Ѳ˘ ǫȴ̑͘ ϾѭǍ͘ ͈ǀѫƽ , ηδԿù , ǫЍĴçմԛ͕Ⱦǫ βкϟ êϔҚ҆յ

P-69

Aspergillus niger NRRL 328 ὲ III ǔÑÝ¦´½ƣʒԒУ|ÈàâƂƣΣ~αʒΏǑӿýնȌąɅȊնȟ̇ʐçն˷δҘ̲ն̚˼ŏǭԋ մ˔ǫäέȾäɼƂյ

P-70

լѺ A. oryzae ὲ´Ë III ÑÝ¦´½ƣʒԒУԄĘǼ csyB ~̴њҧ̅ϝδέш 1ն̳Ō Ҷ 1նſSɚđ 1նƃ˷žf 2ն҇ą ƀ 1
մ1 ȯʘƅϯǫä҆, 2 ̂ǫԲäӧαϯäɼαȾյP-71

Pyripyropene αƣʒ|ԫ Pyr1 ~̫ӵ̴њҧ̅ìȺѫɧն̢δ͢Ҏ, ԯԌԊ˱ մ̂ǫԲ҆յP-72МΥѺὲÕàºÞÎÂ½ terretonin αƣʒԄĘǼ~̴њҧ̅̃δĪǫ 1նĖ҇ȵ˅ նɵ̸͌ý 1ն҇ąƀ 2նøǕƶǮ 1նͪэǠӄ 1նԯԌԊ˱ 1
մ1 ̂ǫԲä҆ն2 ȯʘƅǫä҆յ

P-73

2-«â¼Ü¢ÂâαƣʒԒУ~ƥȉy̴њҧ̅ŷ͘ʥƴնͯȺǿъ, ԯԌԊ˱ մ̂ǫԲä҆յ

P-74

ŤǼŚ peroxide ̳̋ɦʒҨǻl Verruculogen ƣʒԒУ FtmF
ŷ҇ϒ̲ 1նǳ˼ѲǮ 2նե̳ĲĂ 1նԧδҘù 1
մ1 έϟǘɏϟä¦Ó

P-75

ÞÄà­åն2 եӤɗŏϯȀϟϹ°â´åյÞ¬Ü¤´âαƣʒԄĘǼ¤Ü®´å~ҧ̅δôɩ 1, 2ն˷ȬեɎ 1ն̇ˁ˘ 1նɜδ͢ 1նȄ҇ϒǼ 2նԧδҘù 1
մ1 έϟä¦Ó

P-76ÞÄà­åն2 ̂͢ǫäȾյМΥѺ Coleophoma empetri F-11899 ~ FR901379 Պαβǧξ̓~ T-DNA ʫőǧξЋʖ~ҧ̅ǢąƇĦǼնȬδԿĊնфԙԴčնϣӆ͠ɠն̳˷̺͘ մȓȬϕǫäαΣȾյ

10

P-77

ϖѺ|]dà¢¬®àÒ¬â~ˍҡĥγΔ~ʯФϝą ˥նΝǒ Ѵġնȟ͚ ŊɅնƢŽ ġƴնԤĶ եɻ (̂έǫԲέȾäɼαϯ)P-78ÁZsςѺ~¢¶â»°¶Üå±ϓƥ´âÅ¤ԄĘǼ CBP1,CBL1 ~̴њҧ̅ƤδыնǫԙŊǼնôȹġçնԤĶեɻ մ̂έǫäɼαϯյ

P-79ÁZsςѺ~ψѰЌψΫԄĘǼÜÊÜÝå¤àåâ B51 ~ԄĘǼϠǥĢҧ̅ĠR˴Ľʅնȟ͚ŊɅƢŽġƴ͵δơԤĶեɻP-80ÁZsςѺ|]dÁʢʞɿ ҵȞǆǼαβԄĘǼ~ʯФǒƜ̼äƐȴĲ˘äҝ˼խԗ͏ (αΣϟ)

P-81ÁZsςѺ|]dĊΕϋ DNA Ă˷ԥťˌҵȞyϓƥЪʴ\ĵɲѷʹӏùնǫԗĵçն*ˮ͏ŵնДȬžшն̛δѳ մ˘͘ǫӧä*ӧȾǫӧյ

P-82ÁZsςѺ|ʎ̍lÒ¨Þ® MoCV2 y MoCV1 ~αƂȀϋ{͈ӡҧ̅̂ͧ˦ýնͧȬĲçնϬƐˁҎնˮ͏ŵնȗȮɷն̢ȬҘō մӧȾǫԲäӧȀɖյ

P-83ÁZsςѺɢ͇ƂÒ¨Þ® MoCV1 ~ɿΥҧ̅ƕÞ®´âÅ¤Ӕ~̴њҧ̅ǭδ˦ǼնͧȬĲçնϬƐˁҎնˮ͏ŵնȗȮɷն̢ȬҘōմ̂ĉӧȾǫԲäӧյ

P-84ÁZsςѺɢ͇ƂlÒ¨Þ® MoCV3(Magnaporthe oryzae chrysovirus 3) ~ɿΥҧ̅ӬδСƟäͧȬĲçäզąԅǼäϬƐˁҎäˮ͏ ŵäȗȮ ɷä̢ȬҘōմӧȾǫԲäӧȀɖյ

P-85

϶ZsςѺ Magnaporthe grisea |]d̤Σ»Éâ¬â~ĥγÕ¿¯Ôҧ̅ȟկѮƟPȬȶˤéP̟˷̺ùPե˴̺ӾPեø͢˧մˍΈ҆ǫäɼαϯյ
P-86Ágѿ̊ςѺ~ŤαјǼɦʒıӷlМΥѺ Phomopsis sp. (Diaporthe sp.)ǫҝԾčնëԙҶնѷΑ̪ն˴Ɛͱç մȴ̕ǫäαӒյ

P-87¼Öà¨¬gѿ̊ςѺ (Cochliobolus heterostrophus) ~ NADPH Oxidase ԄĘǼщ~̴њҧ͚̅ͤɠġ*նհ҇ϫçնğδƉýն̢δБնδôƇȜմĉǫäԲäӧն*Ϋ ΁ӑϕǫäȀʪΤũϟϹƵյ

P-88¼Öà¨¬gѿ̊ςѺ|]d Cdc42 ԄĘǼ~̴њҧ̅ğδƉýն͚ͤɠġ, ̢δБ, δôƇȜմĉǫäԲäӧյ

P-89ÝՖΓρςѺ_Ť͛lÉ¤´å´âÅ¤Ӕ NIS1 ~̴њҧ̅ő̲̉͠նƤԙ՟вնˡԪ˅նǳԙƶԋնեԙъǿ մĉǫäԲäӧյ

P-90ÝՖΓρςѺ~ςƐɿ|ɹՐxYŤ͛´âÅ¤ӔԄĘǼ LAC2 ~ƥȉƕ̴њҧ̅̇ Шĕնǳδ̉Ϸնǳԙƶԋնեԙъǿ մĉǫԲäӧյP-91

Cryphonectria parasitica RAS3 ´âÅ¤Ӕ~αƂȀäαέȀϋɿӔȬŚŊӤPե̳ʥýPЄƐЧ մȌǕǫä՘β̧äΰǢյ

P-92¾¬կˊςѺ~ʎ̍ǂȇ|]dͥɿԓУϵαʒқƣĢ~̴њҧ̢̅δԾçնΩɚͣ, ̢ȺՍǼ, ͐δĽç, ˸˽ŋ մϪʕǫäӧȀϟϹϯյ

P-93

լѺ A. oryzae |]d AoSO y Stress Granule ~ŕȥǏҧ̅ծ ͼǺնõȬ Ήçնƃ˷ žfմ̂ǫԲäӧαϯäɼαȾյ

11

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13

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Lessons from Yeast –dissection of molecular mechanism of autophagic machinery Yoshinori Ohsumi（Professor, Frontier Research Center, Tokyo Institute of Technology）


14

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Ğ Ğ Ğ Ğ Ğ Ğ Ğ Ğ Ğ Ó°æŀXņRÔá³®Ł³Wzº—ĔOVŔĲĸÔáďĂÚŁ)UŻśűťź—

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bıĿÍŔĲĻÔáďĂÚŔëĹĻĥŐƉįŁÔáďĂÚłƈ1®ŁŞżŬŷØ:(gapjunction)ƈ®Ł5løĂÚ(plasmodesmata)Ľ%āĲĸƈRÔáÂ³®ŀŉŎőŐå=©ĥ¯rļĤŐƉÓ°æŁĔOVŔĲĸÔáďĂÚłƈRÔá³®ĽĲĻŁ³àŀĊîĿm.ŔŋĺĽÜħŎőŐĪƈįőŀĐĴŐÅÎłĤňŏăŕļĥĿĥƉó«ļłƈ«ÝŎŁ A. oryzae Ŕ´ĥĸÅÎļŎĩŀĿĹĸƈĔOVŔĲĸÔáďĂÚŁ)UŻśűťźŀĺĥĻÕĲĸĥƉ+A2
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ŎĩŀĲĸ 3)Ɖ
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łŤŮƁŤŀĿŐĽĔOVŀ'ĖĴŐįĽƈªæŁŔđĮâŀĐĴŐįĽŔŎĩŀĲĸ 4)ƉįŁįĽĩŎƈAoSO ĪŤŮƁŤW¾ŀÔáďĂÚŔ-pĴŐ īŔĴŐ8âwĪÇAıőĸƉAoSO ł 1195 ŗŹųĉĩŎĿŐhSĿũƃŴşøļĤŐĪƈWW ůŻřƃQł ÄŁůŻřƃĪWGĶĵƈ±GƈŤŮƁŤWwŁ'ĖĽĔOVŇŁ¯¼¾Ŀ'ĖŀtîĿĘJŁÙŏýŉŔëĹĻĥŐ 5)Ɖ
ıŎŀ«ÝŎłƈw³ ŀtîĿ MAPK ×úŁŝŰƅŧŀÁ;wŔÇĴũƃŴşø
AoFus3 ĪƈĔOVŁsŔEĦſƃŠ°ŁdGŔÇĴįĽŔï(Ĳĸ 6)ĠňĸğĆUÆPŁ
ì±IñĩŎƈAoFus3 ĪªæŁŔđĮâŔĴŐįĽŔŎĩŀĲĸƉįŁØōŏƈ
15

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VŀĨĥĻ AoFus3 Ľ;ŁdGŔÇĴqbÑlys(microtubule-organizing center)ĐĂũ
ƃŴşø AoApsB ŀĺĥĻŋƈªæŁŔđĮâŔĴŐįĽŔŎĩŀĲĸ 7)Ɖ
«ÝŎłƈAoFus3 Ľ AoSO ĽÁ ´ĴŐũƃŴşøŔŤşſƅűƃŠĲĸØƈķŁĿĩĩŎĲĥĔOVdGũƃŴşøŔ½ïĲĸ 8)ƉŁÄïĩŎƈĔOVłġŁÔá&DUŔĖÌ
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2. J. Maruyama et al. (2005) Biochem. Biophys. Res. Commun. Vol. 331, 1081-1088.
3. Y. Tanabe et al. (2011) J. Biol. Chem. Vol. 286, 30455-30461.
4. C. S. Escaño et al. (2009) Eukaryot. Cell Vol. 8, 296-305.
5. J. Maruyama et al. (2010) Biochem. Biophys. Res. Commun. Vol. 391, 868-873.
6. ŎĞ 2011 jÓ°æ)U³®XšƃŶŖƁƃŤîĖ p.48
7. ġŎĞ 2010 jÓ°æ)U³®XšƃŶŖƁƃŤîĖ p. 54
8. g¹ŎĞ 2011 jÓ°æ)U³®XšƃŶŖƁƃŤîĖĞ p. 51
9. ÃçŎĞ 2011 jÓ°æ)U³®XšƃŶŖƁƃŤîĖ p. 26

Molecular biological lessons about multicellular organization from septal-pore mediatedintercellular communication in filamentous fungi
Jun-ichi MARUYAMAĞ (Dept. of Biotechnol., Univ. of Tokyo)

16

S-2
ԄĘǼ~ичϋϠǥ͜ŪγjqլѺɦʏɦʒūɱН~ҧ̅ȟȺ ϖɠPԛ ԟ́PȟȬ ͠ĂմŔύӈǇ͜Ċԙδβ̧ϯȀϟϹʖյլѺ Aspergillus oryzae P_ǌ~Ęбϋ{՘Ƴβ̧|γZw`qѺxY_P˷
Ѻ~ɦʏɦʒūɱН~ҧ̅PYӷxZ{ZΥ͙xYtqQo~Ɛǆ~çuyjw
P˷Ѻ~ԄĘǼ´å§¸ºâ¥ŹΪ_ĞbPԄĘǼϠǥ|ҧ̅_ҎZ|b^tq
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ku70 ϠǥjPԄĘǼ´å§¸ºâ¥ŹΪ՗ӛϋ|ë˖hnqլѺԩψjqQo
jwʓRPf~լѺ ku70 Ϡǥ̓γZPլѺӞŞūɱǆǼԄĘǼичϋ|ϠǥjqÜÊÜÝå~̫АyլѺ 7 ν̍ѬĢ~ˬȟƂұPo~ӽϳ|]ZwլѺ~ɦʏɦʒ|ԫîlyю\ԄĘǼ~Ϡǥ̓қˆɰfy_x`qQ˷¬âÑ­ÔxPf~̓~ҧ̅^^tw`qլѺ~ɦʏɦʒūɱН|uZwψғlQ
I. լѺŤαǼɦʒ]ѺМěԧūɱН~ҧ̅: լѺӞŞūɱǆǼԄĘǼϠǥ̓ÜÊÜÝå^PӪоϵxY A. nidulans |wˑ|ǝƭhwZŤαǼɦʒūɱНԄĘǼ~PլѺx~å²à¥~Ϡǥ̓ƚɰjqQqPӞŞūɱǆǼx{Z_PG-Proteinčjq¬¥¾ÞĘӿ|ԫîl flbA å²à¥~Ϡǥ̓yPfadA å²à¥~ǧξ̓|uZwĥҚjqQf̓|uZwҧ̅ҎZPլѺŤαǼɦʒūɱН~ǘ˷ЫӚ~ʱȉҎtqyfPլѺ~ŤαǼɦʒūɱНŒĢϋ| A. nidulans yՖĜjwZ_P
G-protein ҧjqѺМěԧ~ūɱН|uZwլѺ A. oryzae y A. nidulans yxξ{twZfy_ϥƷhq 1)Qq˷Ϡǥ̓ÜÊÜÝå^Pˍҡ~ŤαǼɦʒūɱǆ
Ǽ_ҠţhPf~ô|PDNA Ьƣ´âÅ¤Ӕ MED6 Ö¶åÉˮl RNAÑÝÕÜå±~Õ»å´å~ ^P¤àÒ¶âÝÖ»Ýâ¥É¤´å¨å½lԄĘǼ_ƪwZqQ
II. ǫ ҡ ̯ Ց ǖ ̶ ǰ |   Ҡ ţ h  q ˍ ҡ Ӟ Ş ū ɱ ǆ Ǽ ~ ҧ ̅ : fxʓRPլ
Ѻ 7 ν̍ѬĢˬȟƂhnq̓~ĥҚұw`qQo~ĥҚӽϳ|]ZwPhelix-loophelix Ö¶åÉˮlӞŞūɱǆǼxY AO090011000215 ϠǥlyPҤ̓եȒɗxǪˆ~ŤαǼɦʒlfyψҠjqQh|P˷ԄĘǼɤūψΫhnqլѺxPÒÞ¹ȔǬǗǑëxŤαǼɦʒ~ʜūyPѺ̔~ӽŲɦʒ_ҥͻhqQf~Ь̈^P˷ӞŞūɱǆǼլѺ~ŤαǼѺ̔~ɦʒyZtqɦʏɦʒ¬®ºÔ|]
ZwPՊɉ|ԘҞ{Ň`jwZyю\ 2)Q
1) Ogawa M., et al., Fungal Genet. Biol., 47: 10-18 (2010).
2) Jin F.J., et al., Appl. Environ. Microbiol., 75: 5943- 5951 (2009).

3
3
Analysis of regulatory systems for conidial and hyphal developments using comprehensive
gene disruption methods in Aspergillus oryzae
Masahiro Ogawa, Feng Jie Jin, and Yasuji Koyama (Noda Institute for Scientific Research)
Ğ

17

S-3
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˷Ѻ~ςƐɿ̶ʷǧξ̓~ǧξԄĘǼ~ҧ̅^ Kelch Ö¶åÉuԄĘǼմCoKEL1, CoKEL2 յ_ĐϘǂ~̺ɉ{ɦʏŤƂ|ɹՐxYfy_ϥhq(4,5) 
CoKEL1 ЦјƮ˳ĬǾϋ|ɴȟЌ|ȥǏjPԄĘǼ̶ʷ̓ξɉɦʏ~ĐϘǂɦʒjPςƐɿ̶bQCoKEL2 ŤҕԒ͆~ TEA1 ԄĘǼ~ÏÖà¥ԄĘǼxYPŤҕԒ͆yƥ̬|ɴȟЌĬǾϋ|Цј~̨ɿʒԧԌĝ|ȥǏlQf~ǧξ̓®Ü½¡Ü
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ԧ| CoKEL2 _ԫîl~~P̤ΣĢëxf~ЫӚɹՐyj{Z̨ɿ͑ȉ̴̫_Yƞњɿ_ϥƷhqQ
ջյ ÝՖΓρςѺ~ÎÞ¢¬³åÔ̴њyĮőǂȇŤƂ
ţѰԒ͆|]ZwÎÞ¢¬â̴њ~бƣϋ{ҧ̅_ӷwZQ˷Ѻ~ςƐɿ̶ʷǧξ̓~ǧξԄĘǼҧ̅^ÎÞ¢¬³åÔ̴њ_ĐϘǂ~̺ɉ{ŤƂyςƐɿ|ɹՐxYfy_ϥhq(1)QPEX13 ÎÞ¢¬³åÔȥǏ¬¥¾Þˮl´âÅ¤Ӕ~ƛȏĢ´âÅ¤Ӕ PEX5 ~½¸¢â¥´âÅ¤ӔxYPÎÞ¢¬³åÔȥǏ´âÅ¤Ӕ~ƚө|ԫîlQpex13 ǧξ̓ÎÞ¢¬³åÔ̴њ̶ʷ|PћѕԓđҾњ|ξɉαkQqPĐϘǂÕÜ¿âƂ̶`PĮőњǰ[QÕÜ¿âƣʒ~ŧ˳ӽϳ acetyl-CoA ţψΣӔylÑÝ¦¶½ƣʒНxYQacetyl-CoA ĐϘǂɦʒӽϳ|]ZwÎÞ¢¬³åÔ|]dћѕԓ~=?ԓƂ̴њ|īаhyю\PÎÞ¢¬³åÔ̴њ̶ʷ_ÕÜ¿âƣʒ̶ʷqjwZfyϥjqQqPĐϘǂ|]d¥Ý°àåÞ҂ϸ~Ğì^Įő|ɹҞ{Ѣǎɦʒ|ξɉ`qjP
f~fy_PÕÜ¿âƣʒ̶ʷ|ŷ\Įőњ~Ğì~ҞǆxYfy˘^|jqQ
qPћѕԓđҾ̶ʷϥjPςƐɿ̶bǧξ̓~̶ʷԄĘǼ_ PEX5 ƛȏĢ~Ýª¤Ýâ¥|ԫîl PEX22 ~̴њÞ²à¥ԄĘǼxYPÎÞ¢¬³åÔ̴њyĐϘǂŤƂ|ԫîlfy˘^|jqQqPΤІl`fyPf~ PEX22 ´âÅ¤Ӕ_ÎÞ¢¬³åÔ|ȥǏl^x{bPǼǃѺ|Τɶϋ{ЦјȟǂȇxY
 Woronin Body(WB)|ȥǏϥlfy˘^|jqQfxPWB ~ȥǏɿ_ϥhqÎÞ¢¬âǝƭāĩ_{bPÎÞ¢¬³åÔ̴њy WB ~ԫİϥlˍq
18

{ψҠyю\wZQqPΓρςѺ|]d pex22 ̶ʷ̓~ĐϘǂɦʒξɉyςƐɿ̶ʷɦӔ pex13 ǧξ̓yƥЇxYtqQÎÞ¢¬â̴њռյÝՖΓρςѺ~§ÂÔҧ̅ÝՖΓρςѺ 104-T (MAFF240422)Нб~½ÜÉ¼§ÂÔҧ̅ҎtqQ˷Ѻ~§ÂÔª¯П 91Mb ϥjPǼǃѺ~ǝƭāĩyjwÔ£Ֆ[zfςѺ(Blumeria
graminis)|̷cPǫ`{§ÂÔª¯ϥjqQÝՖΓρςѺ~ƥȪ~ C.
graminicola ] C. higginsianum ~§ÂÔª¯opП 57MbP53Mb xYP˷Ѻ~§ÂÔ~Τξɿ_ϥƷhqQqP̍ѬĢ~ЦјȀϋҥȖ^˷Ѻ~̍ѬĢ|]
Zw AT ԎŦ|ȓÌºà¤àÒ¶âՑǖ_Ƣ̍ѬĢ~ϓɥԌŤƍfy_˘^|{tqQçˎPB. graminis |]Zw AT ԎŦ|ȓƘɲԎŦ_§ÂÔ~ 64ճƍPo_§ÂÔª¯~ʦǫ|u{_twZyǝƭhw]Pf[jq§ÂÔâÉßå¬Úâ_ՖĜ~ΫӅxYƞњɿ_ϥƷhqQҾӦ  ÝՖΓρςѺ~§ÂÔҧ̅Ǫӑ̺ЎƌǦմȮȬǫȀյPեԙъǿƌǦմĉԍǫȀյP ϊՐ ӓƌǦմέϟյPթǒъɠƌǦմȮȬαΣϯȀкƣϟϹʖյ|ŕƥϟϹxYQɟγˉΧ1) Fujihara, N., Sakaguchi, A., Tanaka, S., Fujii, S., Tsuji, G., Shiraishi, T., O’Connell, R., andKubo Y. (2010) Peroxisome biogenesis factor PEX13 is required for appressorium-mediatedplant infection by the anthracnose fungus, Colletotrichum orbiculare. Mol. Plant-Microbe
Interact. 23: 436-445.
2) Kubo, Y. (2011) Appressorium function in Colletotrichum orbiculare and prospect for genome
based analysis. in Morphogenesis and Pathogenicity in Fungi Series: Topics in Current
Genetics, vol. 22 Pérez-Martín, José; Di Pietro, Antonio (Eds.), Springer. (in press)
3) Kubo, Y., and Tanaka, S. (2010) Pathogenesis and plant basal resistance in Colletotrichum
orbiculare and Magnaporthe oryzae infection. In Genome-Enabled Integration of Research in

19

4)

5)

6)

7)

8)

Plant Pathogen Systems. (Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J. and Akimitsu, K.
eds), APS Press.
Sakaguchi, A. Miyaji, T. Tsuji G. and Kubo Y. (2008) Kelch-repeat protein Clakel2p andcalcium signaling control appressorium development in Colletotrichum lagenarium. Eukaryot.
Cell 7:102-111.
Sakaguchi, A., Miyaji, T., Tsuji, G., and Kubo, Y. (2010) A Kelch repeat protein Cokel1p
associates with microtubules and is involved in appressorium development in Colletotrichum
orbiculare. Mol. Plant-Microbe Interact. 23: 103-111.
Sakaguchi, A., Tsuji, G., and Kubo, Y. (2010) A yeast STE11 homologue CoMEKK1 is essential
for pathogenesis-related morphogenesis in Colletotrichum orbiculare. Mol. Plant-Microbe
Interacti. 23:1563-1572.
Tanaka, S., Ishihama, N., Yoshioka, H., Huser, A., O'Connell, R., Tsuji, G. ,Tsuge, S., and Kubo
Y. (2009) The Colletotrichum orbiculare ssd1 mutant enhances Nicotiana benthamiana basal
resistance by activating a mitogen-activated protein kinase pathway. Plant Cell 21:2517-2526.
Tanaka, S., Yamada, K., Yabumoto, K., Fujii, S., Huser, A., Tsuji, G., Koga, H., Dohi, K., Mori,
M., Shiraishi, T., O’Connell R., and Kubo, Y. (2007) Saccharomyces cerevisiae SSD1
orthologues are essential for host infection by the ascomycete plant pathogens Colletotrichum
lagenarium and Magnaporthe grisea. Mol.Microbiol. 64: 1332–1349.

Infection structure development and pathogenesis of Colletotrichum orbiculare
Yasuyuki Kubo (Lab. Plant Pathol. Grad. Sch. Life and Environment. Sci., Kyoto Prefectural
University)

20

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Differentially expressed genes under simulated microgravity in fruiting bodies of the fungus
Pleurotus ostreatus. FEMS Microbiol. Lett., 307, 72-79 (2010).

Mushroom formation affected by gravity.
-The gene expression under simulated space environmentYasumasa Miyazaki1, Masahide Sunagawa1, Akira Higashibata2, Noriaki Ishioka2, Katsuhiko
Babasaki1, Takashi Yamazaki2,3(1Dept. Appl. Microbiol., FFPRI; 2Inst. Space Astronaut. Sci., JAXA; 3(Present address) Inst. Med.
Mycol.,Teikyo Uinv.)

23

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Zw¤àÒ¶âäÝÖ»Ýâ¥Ě[ӞŞҺЎ_ͥψ|ҎwZfy_ϥƷhqQofxնՇѬŏ|ǼȊĢɦʒƥҺϋïuϜ˞Ԫ|ԩǷhnfy_x`Ǘ՜˿ĔԩψjնǼȊĢɦʒԩǷ˞|ψΫǧŻlԄĘǼ DNA Ò¤àßȊգxҺ̐j
qQo~Ь̈նǼȊĢɦʒԩǷ˞|ӞŞͥɿ_ë˖lԄĘǼմlog2: >2յ_ 82 ķնqì԰lԄĘǼմlog2: <-2յ_ 34 ķҠţhqQӞŞͥɿ_ë˖lԄĘǼ~ô|ն
fasciclin, galectin, lectin, hyrophobin yZtqЦјǩxŇb´âÅ¤Ӕ¨å½l~_ǪbƪwZqմ10 ķյQf~Ь̈նǼȊĢɦʒԩǷ|]ZwѺМЦјԪϓăĥγ~
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chromatin immunoprecipitation (ChIP)-sequencing |ҧ̅ҪθjնȊգӷwZQ
4'!53[1] U. Kües (2000). Life history and developmental processes in the basidiomycete Coprinus
cinereus. Microbiol. Mol. Biol. Rev. 64, 316–353.
[2] T. Kamada (2002) Molecular genetics of sexual development in the mushroom Coprinus
cinereus. BioEssays 24: 449-459.
[3] J. E. Stajich et al. (2010) Insights into evolution of multicellular fungi from the assembled
chromosomes of the mushroom Coprinopis cinerea (Coprinus cinereus). Proc. Natl. Acad. Sci.
USA 107, 11889-11894.
[4] T. Nakazawa, Y. Ando, K. Kitaaki, K. Nakahori, T. Kamada (2011) Efficient gene targeting in
ΔCc.ku70 or ΔCc.lig4 mutants of the agaricomycete Coprinopsis cinerea. Fungal Genet. Biol.
48, 939-946.
Analysis of molecular mechanisms for multicellular morphogenesis of the homobasidiomycete
Coprinopsis cinerea
Takashi Kamada (Grad. Sch. Natural Science and Technology, Okayama Univ.)25O-1 (P-22)
լѺ A. oryzae ~ MAP ¢¾å± AoFus3 yϓăĥγlˍҡ´âÅ¤Ӕ~̴њҧ̅Ϛѽ ǫӏնõȬ ΉçնÖzgür Bayram1նOliver Valerius1նGerhard H. Braus1նƃ˷ žfմ̂ǫԲäӧαϯäɼαȾն1 §¸ºâ§âǫȀյ
VϑϋWϖ̔αΣ|ɑbĳǾhwZ MAP ¢¾å±նǩԌ^~ʉǝĘӿlfyxЦјͥŻūɱjwZQԒ͆ Saccharomyces cerevisiae |]ZwնMAP ¢¾å±~yu Fus3 ˮɿά|]ZwնʰƣÉàÖâɼЉyЦјҌƣ|ԫîlQМΥѺ~ Fus3p ÏÖà¥ˮɿά|ԫîlfy|ŷ\նĂ̷đҾնςƐɿЇ|]ZwԘҞ{Ň`ˮlQʓRĒŮնA. oryzae ~ Fus3p ÏÖà¥xY AoFus3 _ѺМŎЀyԹǤǽ|ȥǏjնo~ԄĘǼϠǥ̓ѺМʒԧ~ξɉyŤαǼɦʒ~ĞìϥlfyҠţjqQqնAofus3 Ϡǥ̓xԽʰlЦј|ͿѺ_Ęʺlųƣ_ë˖jqfy^նAoFus3 _ԹǤǽčjqЦјԪӶЮ|ԫİlfy_ϥƷhqQf~[{МΥѺ~ Fus3p ÏÖà¥~Ǫ̬{̴њ|]dŤǼ
̴̫|uZwP yz˘^|{twZ{ZQČǅPf~̴̫ҧ˘lqնA. oryzae |]Zw
AoFus3 yϓăĥγl´âÅ¤Ӕ~ƥȉy̴њҧ̅ҎtqQVˎ͜äЬ̈WAoFus3 ~ C ˶Ѐ| TAP(Tandem Affinity Purification)´¥ӶЬjqҌƣ´âÅ¤Ӕն
Aofus3 Ϡǥ̓|ψΫhnqQ´¥γZqЙҚ] LC/MS/MS ҧ̅| AoFus3 y~ϓăĥγϥl´âÅ¤Ӕƥȉjնo~{^x̴њ˵ϛ{ 2 ϵ~´âÅ¤Ӕ|uZwҧ̅ҎtqQRACE ҧ̅|ԄĘ
Ǽ~ĀͻЏǉ_̺jZfyϢҴjq~sϠǥ̓ĥҚjնғΫǔҧ̅ҎtqЬ̈նòяy|ŤαǼɦʒ~Ğìϥjն^uƢR~̓xξ{tqѺМɦʏ~ǧƂϥjqQƥ˞| EGFP Ҍƣ´âÅ¤ӔψΫhnwȥǏҧ̅jqyfնԹǤǽyЦјӔ|½¸¼Υ|ȥǏjwZfy_ҥȖhqQΫǏնëҬ 2 ϵ~´âÅ¤Ӕy AoFus3 y~̴њϋԫİ̥ҫjwZQ
Identification and functional analysis of novel AoFus3(MAP kinase)-interacting proteins in Aspergillus oryzae
Daiki YAHAGI, Jun-ichi MARUYAMA, Özgür BAYRAM1, Oliver VALERIUS1, Gerhard H. BRAUS1, Katsuhiko
KITAMOTO (Dept. of Biotechnol., Univ. of Tokyo,1Georg-August-Universität Göttingen)

O-2
Aspergillus nidulans Ł NO ÞwŀĐœŐŢŮşƂź P450 ŁâĽķŁm.
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VϑϋW ςƐѺ nitric oxide (NO)|ɼЉjwԄĘǼ~ψΫǧƂhnfy|twPNO |Цј͇ɿǅԆjwZQȊԻPςƐɿԒ͆ NO |ɼЉjw 100 Ēë~ԄĘǼ~ψΫǧƂhnfy_ϛţѰԒ͆xPӞŞǆǼ Fzf1p _ NO |ɼЉjwÉÜÐÌÖ¥àÇâ Yhb1p ~ψΫҵȞjPNO ҧ͇lfy_˘^y{twZQçˎPМΥѺ|]ZwPNO |ҵȞhԄĘǼ|uZw yzϛwZ{ZQjq_twPÖ»ÞМΥѺxY A. nidulans ~ NO ҵȞɿԄĘǼ~ƥȉPМΥѺ~ NOѐɿƂ~ŤǼ̴̫έҧlëxԘҞxYQf~q|PʓR DNA Ò¤àßҧ̅ҎZPNO|twψΫԚ_ǣŷl A. nidulans ~ԄĘǼǪˆҠZrjqQqPNO |twɤbψΫ_ҵȞhq¬¼¤àÔ P450մP450յyʱȉhԄĘǼ~ԄĘǼϠǥ̓~αі_ NO ŀ`ĲĻùx7wŔÇĲĸįĽĩŎğ
įŁĆUĪ NO |ȘlѐɿƂ|ԘҞxYfy_ϥƷhqQ˷ϟϹxP˷ P450 ~ NO ѐɿƂ|ԫ̴њyo~ɫų|uZw̥ҫjqQ
Ģ ¤ Ĩ ō ń Ø  ģ æ&Łô®Ŕ GC-MS Ĩōń ESI-MS Ŕ´ĥĻñĲĸĽįŒğšƃŮƂƅƀĽ¢ûĲĻNO ŤŮƁŤŀĨĥĻğãø·)ŀĻ nitrated oleic acid (OA-NO2) Ĩōń nitrated linoleic acid (LA-NO2) Ī(ıőĸĠİĭþğ@ ęŁê¨ŀĨĥĻğNO ŀōĹĻŚƁřƃĉŌſųƅƀĉĪĉ2ıőŐįĽļ³yĲğķőŎĪŦśƃůŻŬŦƃţżƅĽĲĻâĴŐįĽŌÔáŀ¡wŔħŐįĽĪL<ıőĻĥŐĠķįļğÿŁ P450
ŔSäæŀĻ¼Ë½±ıĶğÖ~ħũƃŴşøŔoĸĠįőŔ P450 ƁŪşũƅŧĽĽŋŀ OA-NO2 Ľ6vıĶĸĽįŒğOA-NO2 ĪűŮƂŚƁŶŘƃćļĎíĴŐįĽĪÇıőĸĠįőŎŁįĽĩŎğįŁ P450 ł NO ŀōĹĻ³yĴŐűŮ
ƂãßĉŁ)ñŀĐĲĻĥŐįĽĪÇAıőĸĠ
New fungal cytochrome P450 tolerates nitrite oxide.
Motoyuki Shimizu, Toshihisa Narukami, Yosuke Kamimura, Shunsuke Masuo, Tatsuya Kitazume, Yasunobu
Terabayashi, Momoko Machida, Naoki TakayaĞ (Graduate School of Life and Environmental Sciences, University ofTsukuba)26

O-3 (P-25)
Aspergillus nidulans |]dեͺ˿Ĕìx~Ëàºâ¢¾å± C |Ñ¼å¬®ҵȞʜū̴̫~ҧ̅ΡȬήýնǙŚҘù, ǭδ˘ɵ մ̂ǫԲäӧαϯäɼαȾյ Ëàºâ¢¾å± CմPKCյϖ̔αΣ|եɗ|ĳǾhq°Ýâո®ß¿â¢¾å±xYQ
Aspergillus nidulans ~ PKC ¨å½l pkcA ԄĘǼαі|ɹՐ{ԄĘǼxYfy_ϥhwZ 1)QɥϟϹȋ|]ZwĥҚhq pkcA ͺɗʎƛɿ̓ 30Dxԙαǔ̓yƥ̬~ғΫǔϥl_, 42Dxαіlfy_x`{ZQ42D|]Zw pkcA ͺɗʎƛɿ̓~ŤαǼx, ӳɉ~ŤαǼψѰ˞|ҠΖ
̨ɿαԧ_Ӳôxļ̹j, ψѰЌ~ɦʒ_Ҡ{ZQh|Pf~y` DNA ~қҚӗf_, o~ɮlc DNA _Ťҧhq 2)QpkcA ͺɗʎƛɿ̓~ŤαǼ 42DxǗ՜jqy`, Ñ¼å¬®ҵȞjqЦј|Τξϋ|ҠͥɿԓУմROSյ~҂ϸ, DNA ~ˌΡƂ_Ҡqfy^, եͺ˿Ĕì|]ZwψѰ˞| PkcA _ǰͥlyÑ¼å¬®_ҵȞhfy_ϥƷhqQPkcA |twūɱhfy_ϥƷhwZ MAP ¢¾å± ®¦å½~Ñ¼å¬®ūɱ~ԫî|uZw̥ҫlq, f~ MAP ¢¾
å± ®¦å½~̫ʒǆǼ¨å½l bckA, mpkA op~̶ǰ̓~ҧ̅ҎtqQf~̶ǰ̓ӳɉ~ǗǑ|]Zw 42Dxαіlfy_x`{^tqQh|, f~̓~ŤαǼ 42DxǗ՜jqyf, ROS ~҂ϸ, DNA ~ˌΡƂ_ҠqQf~fy^, եͺ˿Ĕì|]Zw PkcA Ñ¼å¬®~ʜū|ɹҞxY, f~̴њ BckA, MpkA ƪ MAP ¢¾å± ®¦å½|ĬǾlfy_ϥƷhqQ1) Ichinomiya, M., et al., (2007) Biosci. Biotechnol. Biochem., 71, 2787-27992) ΡȬ, ˒˷ӧѯƂȀė 2010 ɍɗǫėҽ΄Ҟ˓Հ, p213

Analysis of PkcA function in the suppression of apoptosis under heat stress condition in Aspergillus nidulans
Takuya Katayama, Hiroyuki Horiuchi and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)

O-4 (P-31)
МΥѺ Aspergillus nidulans |]d<-1,3-¥Þ agsA, agsB ĂԘϠǥ̓~̴њҧ̅âƣʒԒУԄĘǼ agsB Ϡǥ̓]϶ѿ̞ 1, ƤҠƻ 2, ԯԌ˅ʇ 1,2մ1 ̂ƃǫԲӧäαΣβ̧Ŵʒ, 2 ̂ƃǫä˵̀ϟյ
ʓR, Aspergillus nidulans ~ЦјǤ̫А¬¥¾ÞĘӿЫӚ|uZw, MAP ¢¾å± MpkA ЫӚôɸ|ҧ̅ӷw`qQfx|, ˷Ѻ~ MpkA ЫӚ, ţѰԒ͆|ǾǏj{Z<-1,3-¥Þ âƣʒԒУ
մAGSյԄĘǼ agsA, agsB ~ӞŞūɱ|ΤƂjwZfy˘^|jwZQq, ˷Ѻ|]d 2 ϵ~ AGS ԄĘǼ~̴њ˘^|lq, agsA Ϡǥ̓, agsB ψΫʜū̓γZw AGS ԄĘǼ~̴њҧ̅ӷw`qQo~Ь̈, agsA ԄĘǼӳɉαі˿Ĕìx yz̴њjwZ{Zfy, ˷Ѻ|]d<1,3-¥Þ â~ƣʒ|ö| AgsB _ԫîjwZfy_ϥƷhw`qQj^j{_, agsB ԄĘǼ~ѧ̾ɿ] AGS ԄĘǼȅŒ̶ʷ̓ƚɰ~ƞƩ|uZw˘^|hwf{^tqQČǅ, fxƚɰ_x`wZ{^tq agsB Ϡǥ̓~ӵʒ|ʒŶj, agsB Ϡǥ̓~ғΫǔҧ̅Ҏtq~xo~Ь̈|uZw
ǝƭlQ agsB Ϡǥ̓, Congo Red |Șlʎƛɿ~ë˖{z agsB ψΫʜū̓xҴqΤɶϋ{ғΫǔϥjqQq, ЦјǤʒŤʣţj, NMR ҧ̅Ҏtqyf, <-1,3-¥Þ â~¬¥¾Þ̥ţ
h{^tqQf~fy^, agsB ԄĘǼ<-1,3-¥Þ â~ƣʒ|ɹՐxYfy_ɤbϥƷhqQh|, agsA Ϡǥ̓Ҥ̓yjq agsB Ϡǥ̓~ӵʒұ, Aspergillus ȪѺ|]Zwŧw AGS ԄĘǼȅŒ̶ʷ̓ƚɰjq~x, f~̓~ғΫǔҧ̅~Ь̈|uZwюȖjqQմ˷ϟϹαϟ°â´åʽʶƛdqQյ
Analysis of the disruptant of <-1,3-glucan synthase gene agsB and the double disruptant of agsA and agsB in
Aspergillus nidulans
Azusa Inaba 1, Akira Yoshimi 2, Keietsu Abe 1,2( 1 Grad. Sch. Agric. Sci., 2 Tohoku, Univ., NICHe.,Tohoku Univ.)

27

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mǷ|  '3 ~   |ϓƥ{ )5 ԎŦ¬´¦ȞőlfyxP  ~ԄĘǼψΫ ʜūj q   Ԅ Ę Ǽ ψ Ϋ ʜ ū ̓ 3,@ -84   ɰ q Q ̷ | ғ Ϋ ǔ ~ ҥ Ȗ  Ҏ t q y f  P 3,@ -84  PȔǬǗ՜˞|ƏZ͊ôѺМ~ȫɦʒj{Zfy_˘^y{tqQӖ̐ǔՅǼՕɴԦ"γZwҳЦ{ҥȖҎtqyf 3,@-84  Ϝbξɉ|ŤȭjqѺМ_ǪbҥȖhPѺМȒɗԙἀ"! y͈ӡjwĞìjwZqQh|ӱӽǔՅǼՕɴԦ#γZwҥȖҎtqʖP3,@-84  ЦјǤ|ǾǏlǩѡ276*4/&:*4yŚѡ-11*4/&:*4~˘Ϣ{Ƅũ_ţ̀mPԙἀy͈ӡjwЦјǤ_҄b{twZfy_˘^y{tqQ3,@-84  xѺМƥǦχϘjwZÉÊÝÞȫ+-'4275/&:*4_̿zҠ{^tqfy^PÉÊÝÞȫ~͸ȠxѺМƥǦ_χϘţ̀mP
ξɉ{Ťȭ|Ѧtq~x{Z^yʱͻhqQh|ǩԌ
0$ 0/ ~ЙҚ ((  3,@-84 ʹŷjғΫǔ~ҥȖҎtqʖP͊ôѺМ~Ԛ_ǣŷjP" ҥȖxҠqϜZξɉ{ѺМ~Ťȭ_͸ȠjP# ҥȖxÉÊÝÞȫ_ՕҀ|ǣŷjPғΫǔ~ǅɲ_ҠqQ
f~Ь̈^P¬´¦ (( ЦјǤ~̫ʒƕѺМƥǦ~χϘ|ԫƞњɿ_ϥƷhqQA Lentinula edodes laccase (Lcc1) influences their mycelial morphology
Keiko Nakade and Yuichi Sakamoto (IBRC)

O-6 (P-40)΢ѶŕαМΥѺ Epichloë festucae ~ĞŤǼԚ G ´âÅ¤Ӕ Cdc42 ƕ RacA ~̴њŤƂ|ԫlϟϹ̩ԙƖ՟նЂ˷ǫƬմƦǫԲäαƱӧȀյE. festucae Áϯ΢Ѷ~ЦјԪԺxαіjնŕαԫİϢϾlМΥѺâ½É¼xYQfx|նE. festucae ~ͥɿԓУαʒԒУ NoxA _ŕα˞~̤ΣŚѺМ~αіūɱlfyնūɱǆǼ NoxR yĞŤǼԚ G ´âÅ¤Ӕ RacA _նNoxA |ͥɿԓУαʒ̺|ūɱlfy_ҧtwZQqԒ͆xЦј̨ɿ~ϢϾ|ԫîl BemA/Cdc24 _նNoxR yқƣĢɦʒjͥɿԓУαʒūɱlƞњɿ_ϥhwZQČǅնRacA ] RacA yեZϓƥɿϥl Cdc42 ~̴њ͈ӡҧ̅jqQYeast two hybrid ͜γZq Nox ūɱǆǼy~Ьƣҧ̅|նRacA  NoxR y Cdc42  BemA y N ˶Ѐľ~ÓÂԓčjwΤξϋ|Ьƣlfy_ϥhqQracA Ϡǥ̓xնѺМŤȭ_ǣŷl~|Șjնcdc42 Ϡǥ̓xԌŤϋ{ѺМЦј~Ѣɣ_ҴqQq racA Ϡǥ̓նȐö̤ΣŚxξɉ{ѺМαіϥjw̤Σ̊̾hn~|Șjնcdc42 Ϡǥ̓Ȑö̤ΣyŕαԫİϢϾx`qQcdc42 Ϡǥ̓xԙἀy͈ӡjwͥɿԓУαʒԚ_ǣŷjqfy^նCdc42 _ͥɿԓУαʒ~Ӈ~ūɱǆǼxYfy_ϥhqQf~Ь̈նCdc42 y RacA _Ёƣϋ|Ň`նͥɿԓУαʒѺМαіūɱjwZƞњɿ_ϥƷhqQ
Functional analysis of small G proteins Cdc42 and RacA from fungal endophyte Epichloë festucae
Yka Kayano, Daigo Takemoto(Grad. School Bioagr. Sci., Nagoya Univ.)

28

O-7
ѺՖ|]dÆ®¼âԄĘǼŚâ¼àâ~ӷƂҝδ͢ɂնȢ ɽԞ մ̂ǫԲäӧȀαƱϯȀϟϹϯյαͥΰ~ǫƈԒ͆xӽglǼǃѺՖÆ®¼âԄĘǼ|â¼àâʧqmնМΥǼǃѺՖʤǼѺՖâ¼àâʧuQѺՖ~Æ®¼âԄĘǼ|]dâ¼àâ~ŤɄ͈ӡjwѺՖ~ӷƂyÆ®¼âԄĘǼâ¼àâ~ԫİ|uZwюȖjqQ24 ϵ~ѺՖ~Æ®¼â H2A, H2B, H3, H4 ԄĘǼô|ǾǏl
305 ķ~â¼àâ~ʫőĝц 85 Ћʖ͑ȉjնʤǼѺՖ| 7 ЋʖyМΥǼǃѺՖ| 9 Ћʖ~Ï¸¼®Ñ¸¼˘^|jq_նf~Нбщxŕӳ~Ï¸¼®Ñ¸¼ 1 Ћʖj^ǾǏj{^tqQtwնѺ
Ֆ~Æ®¼âԄĘǼŚâ¼àâ~Ǫbξ{ӗͽʧtwZyю\նѺՖ|]dŕӳϩŎ|ǾǏjqâ¼àâ~ˆ̨wȠ{Zyю\մ1յQqնâ¼àâʫőՑǖ~ǡǘԎŦ͈ӡ|ǘv`նξ{ʫőĝцԪ|]dâ¼àâ~͋ɌĘʺӗftw{Zyю\qմ2յQl{sնԒ͆αͥ_ôɸ~ǼǃѺՖӷƂ~ӽϳxÆ®¼âԄĘǼ^â¼àâ{bjնМΥǼǃѺՖyʤǼѺՖΦϾ|â¼àâΨɰjqyю\Q(1) Yun C-S, Nishida H (2011) PLoS ONE 6: e16548. (2) Nishida
H, Yun C-S (2011) Mobile Genetic Elements 1: 78-79.

Evolution of introns in fungal histone genes
Hiromi Nishida, Choong-Soo Yun(Graduate School of Agricultural and Life Sciences, Univ. Tokyo)

O-8 (P-52)
լѺ A. oryzae ~Ѻ̔ɦʒıӷǆǼ SclR ~ӽŲψΫ̓γZqѺМҌƣњ~ҧ̅Ʋδձǭ, ԛ ԟŀ 1, ȟȬ͠Ă 1, õȬΉç, ƃ˷žf (̂ǫԲäӧαϯäɼαȾ,1 ԙδβϟ)լѺ A. oryzae ˮɿðđ_Ҡu^tw]m,қˆ~̓~Ŋѫ{ғΫǔƥ˞|ł\q̓~іϵŹΪϋ|Ҏ[fy_ǈՃxYQj^j,§ÂÔҧ̅|ˮɿάԫӶԄĘǼufy_˘^|{,ˮɿάҎ[ƞњɿ_ϥhwZQçˎx,A. oryzae |]ZwѺ̔(sclerotium)~ɦʒњ,ѺМҌƣњ{z_ĞìjwZfy_ˮɿðđ~ψҠǈՃ|jwZçǆyю\QѺ̔yѺМ_ҌƣхӫjwȒՀjqƏѡ~ȟЖǟxY,o~ǩҠˮɿðđ~̫ӵxYǼǃ̓(perithecium)yĜwZQѺ̔Śxʰƣ
ǔ~ξ{Ѻ̓~ѺМ_Ҍƣj,op~̔_ƥçЦјŚ|ǾǏl,̔Ҍƣä͸ˆŤҕЫwˮɿјǼ_ɦʒh~x{Z^y˳ɭhQj^j,fx A. oryzae xѺ̔ɦʒѺМҌƣ|ԫlϟϹ_yzӷxZ{Zfy^,o~ˮɿάњ|ԫlϛҠՊɉ|újZQ˷ϟϹx,̔opлѬ]ӕѬҋŏxƞҢƂlq~ҌƣԄĘǼ sC ԄĘǼɘ|ʫőjq̓|,Ý­â/Ü¬ÞҞ͍ɿ(ΔpyrG)q»¿âҞ͍ɿ(ΔadeB)Đîjq̓ĥҚjqQojw,f~̓ȔǬǗǑxȘȲǗ՜,jbͭĢǗǑxͳƣǗ՜hnfyx,ѺМҌƣњҺqQo~Ь̈,]ă
Z~̑՜Ҟ͍ɿ_ϓҙhˬȠǗǑxαіƞњ{̓ƚɰj,o~ѺМҋŏՕɴԦxҥȖly̔|лѬäӕѬòˎ~ҋŏ_yqQĒëξ{̓~ғΫǔ_ƥç~̓|Ϋqfy^,A. oryzae |]dѺМҌƣњҧ̅l¬®ºÔϢϾlfy|ʒŶjqQΫǏ,Ѻ̔ɦʒıӷǆǼ SclR ӽŲψΫhnfy|,ѺМҌƣњ~Ź̈̥ҫjwZQ
Analysis of hyphal fusion ability by using the strains overexpressing SclR, a promoting factor for sclerotia
formation in Aspergillus oryzae
Ryuta WADA, 1Feng Jie JIN, 1Yasuji KOYAMA, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo, 1Noda Ins. Sci. Res.)

29

O-9
լѺ|]d HECT ÙÇ¢¶âÝ¡å± (hulA) ˿ĔϋψΫ̓~ĥʒδôίɁնˍӂȡɠնĄƯžý մ̂ƃǫԲӧäαΣβ̧Ŵʒյ լѺǪ̬{ǪКՖŤҧԒУщαβl~~Po~ψΫ¥Þ¨å®|´ÐÜ¼ʜūƛd

Qo~qP¥Þ¨å®ʜū̴̫ҧ˘jP¥Þ¨å®ʜū_ҧԳhqȐö̓іϵlfyxǪКՖŤҧԒУ~եαβ_ƞњy{y˳ɭhQ¥Þ¨å®ʜūɑǖūɱǔӞŞǆǼ CreA |twūɱh
PÖ»ÞМΥѺ Aspergillus nidulans xPCreA ~̴њ_ÙÇ¢¶âäѝÙÇ¢¶âƂ|ūɱh
fy_ϥƷhwZQqPCreA ~ÙÇ¢¶âƂ| HECT ÙÇ¢¶âÝ¡å± HulA _ԫîlyʱȉhwZ~~PҳЦ{ҧ̅ҎwZ{ZQofxP˷ϟϹxլѺ|]d HulA ~̴њҧ̅lfyϑϋyjqQ ţѰԒ͆|]ZwPhulA å²à¥xY RSP5 _αіɹՐԄĘǼxYfy_ϛwZfy^
PhulA ˿ĔϋψΫ̓ĥʒlfyyjqQլѺ~ hulA ëͦՑǖ 5´-ՊьҮՑǖ|ÝÐ®¸¶_ǾǏl nmtA ËàÖå´å|цʴjPÝÐ®¸¶ĬǾϋ| hulA ~ψΫ_ūɱh˿ĔϋψΫ̓ĥʒjqQĥʒjq˿ĔϋψΫ̓~αіҥȖjqЬ̈PψΫʜū˿ĔxY¶Óâʹŷ˿Ĕìxαі_ҀjbʜūhPŤαǼɦʒњ_ȅŒ|ǰqQf~fy^PլѺ|]Zw HulA αіqŤαǼɦʒ|ɹՐ{ǆǼxYfy_ϥƷhqQČɮPĥʒjq˿ĔϋψΫγZw HulA ~¥Þ¨å®ʜū~ԫî|uZwҧ̅Ҏ[ĀȉxYQ
մ˷ϟϹαϟ°â´åTÂÍå¬ÚâŴţǘϤϋϟϹʱӷā̧U~ʽʶƛdwҎqQյ
Construction of the conditional expression strain for HECT ubiquitin ligase (hulA) in Aspergillus oryzae.
Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ., Univ.)

O-10 (P-60)
լѺӞŞǆǼXlnR~ҵȞΣӔĬǾϋÝâԓƂyo~αέȀϋʌъϝȺ ƮɌ, ԙƜ Ϩơ, ԛõ ĉǼ, ŷ҇ ԿǦպ, ȟ̇ ƶǮմƦǫԲäαƱӧ, պƦǕǫäӧյ
VϑϋWլѺ Aspergillus oryzae ӞŞǆǼ XlnR , ҵȞΣӔ¢¬àå®|ɼЉjw¢¬Ü¾å±,°ÞÜå±ԄĘǼщ~ӞŞͥɿƂlQfx|, XlnR _ҵȞΣӔĬǾϋ|ÝâԓƂĵ՛hfy˘^|jqQ˷ϟϹx,XlnR ~ҵȞΣӔĬǾϋÝâԓƂ~ҳЦ phosphate affinity SDS-PAGE γZwҧ̅jնq,ÝâԓƂ~αέȀϋʌъ~ҧ˘ϑʨjwնÝâԓƂ_ XlnR ~Ťҧ DNA Ьƣ|î\ɪՍҧ̅jqQVˎ͜yЬ̈Wc-myc Ҍƣ XlnR ψΫlլѺ~Цјʣţͭ phosphate affinity SDS-PAGE |īj,®
´âÊà¸ºâ¥ҧ̅| c-myc::XlnR ̥ţjqQo~Ь̈,ՊҵȞ˿Ĕì|]Zw XlnR ÝâԓƂßÍÞ~ξ{ 3-4 ϵ~ŤǼϵyjwǾǏjնҵȞ˿Ĕì|]ZwÝâԓƂßÍÞ~ë˖jq 2 ϵ~ŤǼϵ| ƙ˾jqQf~Đŷϋ{ÝâԓƂƞӰϋx,¢¬àå®~Գƒ|ՊҵȞ˞~ÝâԓƂßÍÞxĞìjqQÝâԓƂßÍÞ~ԀZ XlnR ~Ȅȉɿ|ɪՍî\{^tqQChIP Assay ] XynF1 ~ËàÖå´åՑǖËàåÊyjq EMSA |,Цјʣţͭô~ XlnR ¢¬àå®ՊĬǾϋ| DNA Ьƣlfy_ϥhqQĒë^,XlnR ɉ| DNA ë|ǾǏj,¢¬àå®ĬǾϋ{ƞӰϋÝâԓƂĵ՛|ͥɿƂh,ìͦԄĘǼ~ӞŞҵȞlyю\Q˷ϟϹ~çԌPαϟ°â´å~ÂÍå¬ÚâŴţā̧|ʽʶƛdwҎq~xYQ
Inducer-dependent phosphorylation of the transcriptional activator XlnR

and its physiological role in

Aspergillus oryzae
Shuhei Ishikawa, Yuji Noguchi, Kyoko Kanamaru, Masashi Katoպ, Tetsuo Kobayashi (Graduate Sch. of Bioagric. Sci.,
Nagoya Univ., պSch. Agric. Sci., Meijo Univ.)30

O-11 (P-61)
Trichoderma reesei ~°ÞÜå±αβ|ԫîl¬¥¾ÞĘӿԫӶ´âÅ¤Ӕ~ʯФ˒ìϭҎնƝȺԶО, ͰӂѲƿ, ɻδ͢čնȟЄƐ ͷ մԧȮʚϯǫäαΣյ МΥѺ Trichoderma reesei նЦјǩ°Þàå®o~ҵȞĢ|ɼЉjw°ÞÜå±ҵȞϋ|αβlQj^jնҵȞ~ŧ͂Էx°Þàå®íͿɿxϒʰЦјѡӳӽx`{Zfy^նz~[|Цјǩ°Þàå®ҴҿjwZ~^í˘xYQqնfx~ϟϹ^ŧψ~°ÞÜå±ҵȞαβ̫ʒϋ|ψΫjwZ°ÞÜå±|twŤҧhqɴԚ~°Þàå®ŤҧβΣ_ɟ`ԛy{ɟ`ӗfhyZ[Ö»Þ_ʳƹhwZ_նo~ҳЦ˘^|hwZ{ZQofx˷ϟϹxն°ÞÜå±ҵȞΣӔ~ƚөЫӚ]ǘӔҴҿ|ԫlϛҠɰfyϑϋyjwնҵȞψΫ|ԫl¼Üâ®Ñå´å~ƥȉұqQT.reesei §ÂÔ»å´Íå®Ś|ǾǏlŒ 9129 ԄĘǼ^КӔӥӯ|ԫîlyʱȉh¼Üâ®Ñå´åԄĘǼʣţjնÒ¤àßγZwϵR~ΓУͽ|ȘlψΫɼЉҧ̅ҎtqQo~Ь̈ն°ÞÜå±ԄĘǼyƥҺjwψΫjwZտķ~¼Üâ®Ñå´åҠţjqQf~¼Üâ®Ñå´å|uZwն°ÞÜå±ҵȞ~ԫî˘^|lq|ƢԄĘǼ~Ϡǥ̓̫АjնÇ°ÞǾǏì|]d°ÞÜå±αβɿ~ҧ̅ҎtqQo~Ь̈նYԄĘǼ~Ϡǥ|ĚZ°ÞÜå±αβ
њ~ҀjZĞì_ҥȖhqQΫǏնf~Ϡǥ̓γZw°àÝ©К~ƚө]°ÞÜå±ԄĘǼψΫ|uZw~ҧ̅ҎtwZQ
Functional analysis of genes encoding a putative cello-oligosaccharide transporter in the filamentous fungus
Trichoderma reesei.
Hideyuki Kusaka, Takanori Furukawa, Eiji Fukaya, Yousuke Shida, Wataru Ogasawara(Deot. Bioeng., Nagaoka Univ. of Tech)

O-12 (P-85)
϶ZsςѺ Magnaporthe grisea |]d̤Σ»Éâ¬â~ĥγÕ̅¿¯ÔҧȟկѮƟPȬȶˤéP̟˷̺ùPե˴̺ӾPեø͢˧մˍΈ҆ǫäɼαϯյ̤Σ|ʞѺɿҊϊӔ|αĢԭɱ̴̫_ǾǏlQʓR̤ΣʞѺɿҊϊӔ~ 1 uxYÜ¬¾ὲ»Éâ¬â BJ-AFP1 _P϶ZsςѺ Magnaporthe grisea |ȘjեZʞѺͥɿϥjPo~ԻPЦјѡѝŤ̨ƂPͥɿԓУϵ(ROS)~҂ϸƕѺМ_ÄÞåâ̫ӵ~ɦʒ_ӗffy˘^yjwZ˷ǫėxPBJ-AFP1 ʹŷɮŧ˳~ M. grisea ѺМƔјǼ~ɼЉ|͟ϑjPҧ̅jqЬ̈|uZwǝƭl
QЪʴ\Ԓ͆ Pichia pastoris ŪγjPψΫäЙҚjq BJ-AFP1  M. grisea |ʹŷjqyfP15 Ťɮ|ЦјѡѝŤ̨Ƃƕ ROS ~҂ϸ_ӗfP˞ԪЫӽyŕ|ѝŤ̨Ƃ~ųƣƕ ROS ǣŷjqQ
qPјǼ| BJ-AFP1 ĥγhnqy` 15 ŤxЦјѡѝŤ̨Ƃ_ӗfPh|ӽŲԚ~ BJ-AFP1 ʹŷlfyxјǼ~Ϡҕ_ҥȖhqQqPBJ-AFP1 ʹŷ|ѺМ~ÄÞåâ̫ӵɦʒPեΎɗ
BJ-AFP1 |ȘjϜ˞Ԫxɦʒhfy_˘^y{tqQqPËà¼ËÜ®¼ƂjqѺМ|
BJ-AFP1 ĥγhnqy`|PBJ-AFP1 ĞʎƛɿϥjqQf~fy^ЦјǤë| BJ-AFP1 ʎƛɿ͑ȉlǆǼ_ǾǏjwZƞњɿ_ϥƷhqQ

The mechanism of action of the plant defensin BJ-AFP1 in Magnaporthe gisea
Yoshifumi Oguro, Harutake Yamazaki, Masayuki Nashimoto, Masamichi Takagi, Hiroaki Takaku(Applied Life Sci, Niigata Univ. of Pharmacy and Applied Life Science)31

O-13 (P-83)ÁZsςѺɢ͇ƂÒ¨Þ® MoCV1 ~ɿΥҧ̅ƕÞ®´âÅ¤Ӕ~̴њҧ̅ǭδ˦ǼնͧȬĲçնϬƐˁҎնˮ͏ŵնȗȮɷն̢ȬҘōմ̂ĉӧȾǫԲäӧյMagnaporthe oryzae chrysovirus 1(MoCV1)ÁZsςѺ|ʎ̍jնȐöѺ|αіԮȍɢ͇ƂӗflÒ¨Þ®xն4~5 ŤЎ~Ă˷ԥ RNA §ÂÔˮlάɦ~Þ®xYQMoCV1 |ʎ̍jqÁZsςѺЦјŚxͭј~єǫƂՓЖƂyZtqξɉ{ɦʏΫjն MoCV1 Þ®ͿͭÞ®ÉÝå~ÁZsςѺ|ʹŷlyѺМ~Ѽр_ҠQ
MoCV1 ʎ̍̓~ѺĢƙԚƧëϑϋyjwÓ¿­×åxǗ՜jqЬ̈նǗǑԚն̤ѺԚƕǗ՜˞Ԫ_ƥk˿Ĕx¨ÞÍâγZqʪy[Ǘ՜y͈ӡjwնП 20 ĸϳɗ~ѺĢԚ_ɰqQ̷|ѺĢ^
MoCV1 Þ®ЖǼЙҚjw SDS-PAGE ]f{tqЬ̈ն14 ˒ԪǗ՜jqѺĢὲ~Þ®ʒŤն
2 ˒ԪǗ՜jq~y͈ӡjwնÞ®̫ʒ´âÅ¤Ӕ~ª¯_ȟhb{twZqQɯtwնÞ®
ЖǼ̫ʒl´âÅ¤ӔնǗ՜˳Ԫ_ԧ˳Ƃlyçȉª¯|ŤҧմËà°¸¬â¥յhfy_ϥƷhqQqնÁZsςѺ~ɦʏξɉ|ԫîlÞ®ԄĘǼʯФlұyjwնÅâԒ͆Ūγjq MoCV1 Þ®ԄĘǼψΫН~̫Аyҧ̅ҎtqQo~Ь̈նÞ®´âÅ¤Ӕ~çuնԒ͆Цј|αіíѫ{z~ɦʏξɉɟ`ӗflfy_ϥhqQ
Functional analysis of viral proteins of MoCV1 (Magnaporthe oryzae chrisovirus 1) which confers hypovirlencetraits to rice blast fungus.
Tokomo Ohta, Syunichi Urayama, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka, Hiromitsu Moriyama.
(Dept. Agric., Tokyo Univ. of Agric. & Tech.)O-14 (P-90)
ÝՖΓρςѺ~ςƐɿ|ɹՐxYŤ͛´âÅ¤ӔԄĘǼ LAC2 ~ƥȉƕ̴њҧ̅̇ Шĕնǳδ̉Ϸնǳԙƶԋնեԙъǿ մĉǫԲäӧյÝՖΓρςѺմColletotrichum orbiculareյնÝϯ̤Σ|ςȍɟ`ӗfl̤ΣςƐМΥѺxYQ˷ѺնÕÜ¿âƂjqĐϘǂɦʒjwȐö̤ΣĮől_նf~Įőӽϳ|]ZwնĐϘǂ~ÕÜ¿âƂɹՐxYQČǅն˷Ѻ~ςƐɿԫӶԄĘǼyjwնŤ͛ǔÜ¸

å±̬ԄĘǼ LAC2 ƥȉjqQ

LAC2 ~̮ϋϠǥ̓|]ZwնĐϘǂ~ÕÜ¿âƂՕҀ|Ğìjw]P¢ØÝ~ςƐɿǰwZqQGFP γZqßÑå´åҧ̅նLAC2 ԄĘǼĐϘǂɦʒӽϳ|]ZwɤbӞŞҵȞhնo~ьҮβΣնЦјǩŤ͛hfy_ϥƷhqQh|նŤ͛¬¥¾ÞՑǖ̶ǰhnqǧξǔ
LAC2 նlac2 Ϡǥ̓ϓҙx`{^tqQqնlac2 Ϡǥ̓նo~јǼ~Ѭ_ԙἀyξ{twZqQÁZsςѺմMagnaporthe oryzaeյ~ LAC2 ÏÖà¥ÝՖΓρςѺ~ lac2 Ϡǥ̓ȞőjqǞƣնo~ŤαјǼ~Ѭǅɲjq_նςƐɿyĐϘǂ~ÕÜ¿âƂǅɲj{^tqQf~Ь̈նLAC2 |јǼ~ѬУyĐϘǂ~ÕÜ¿âαƣʒնƥçƘɼx{bնLAC2 ~ǘӔƋçx{ZfyϥƷjqQh|f~Ь̈նLAC2 _ԫîlјǼ~ѬУαʒςƐɿ|ɹՐx{ZfyϥjqQ {]ն˷ϟϹαϟ°â´åTÂÍå¬ÚâŴţǘϤϋϟϹʱӷā̧U~ʶŸƛdwҎqQ
Isolation and Functional Analysis of a Secreted Protein Gene LAC2 Required for Fungal Pathogenicity ofColletotrichum orbiculare
ShaoYu Lin, Shiho Okuda, Tetsuro Okuno, Yoshitaka Takano

32(Grad. School of Agriculture, Kyoto Univ.)

O-15 (P-91)
Cryphonectria parasitica RAS3 ´âÅ¤Ӕ~αƂȀäαέȀϋɿӔȬŚŊӤPե̳ʥýPЄƐЧ մȌǕǫä՘β̧äΰǢյ¤Ýљ̊ςմchestnut blightյPðιêǫ̤Σς~պuyjwˮƦxPΤ|ДǌxÅÜ¶ȬǑx̨wŊſxYtq¤ÝմAmerican chestnutյƈðО~Ԫ|āȊëŒ΀hn z~ҔȍqjqQo~ƐǆМΥѺ Cryphonectria parasitica P1900 ɍՎ|_ǌ^¿ØåÛå¤|ӋΣ|ĐϘjwϱőhqyZ[QΫǏPC. parasitica ~§ÂÔҧ̅ Щÿjw]Phi{Ҧɗ^̤Σ~ςɶψΫÕ¿¯Ô~ҧ̅]o~ūɱˎ͜_̥ҫhwZQ
C. parasitica ~ RAS |ԫjwPlx| RAS1PRAS2 _ϢҴhwZ_P˷ϟϹxˍq|Ҡţhq RAS3 |uZwPαƂȀäαέȀϋ̴њҧ̅ҎtqQRAS3 ď~ Ras ԫӶ´âÅ¤Ӕ| ŕӳjwҴ C ˶ЀՑǖ~ÉÞÁ¬ÞƂԌĝմCAAX Boxյ̶bfy_ǫ`{Τɶxնo~ΔxŻΣ~Rin ] Rit y~ŕӳɿ_ҴqQRAS3 ¨å½l ras3 ԄĘǼ~Ϡǥ]ӽŲψΫ_ѺМʒԧŤαǼɦʒ|ƕlɪՍ̥ҫjqQGFP Ҍƣ RAS3 ψΫhnwPo~ȥǏ|uZwҥȖjqyfPЦјғȫմЦјѡëյ|ɤZҋŏ_ҴqQC ˶Ѐ 32 ÓÂԓ̶ǰjq RAS3 Pf~ȥǏϥhmPЦјŚԌŒĢ|Ť˃jwψΫjwZqQf~fy^PC ˶Ѐ 32 ÓÂԓ_ÉÞÁ¬Þǘ|˫ѡЬƣ|ȑîjwZƞњɿ_ϥhqQqPRAS3 ~ GTP Ьƣњ GTPase ͥɿЇ~αƂȀϋɿӔҺq|PǫѠѺôx RAS3 ψΫjPЙҚjqQ
Biochemical and physiological functions of Cryphonectria parasitica RAS3
Yuki Yamauchi, Takuya Takahashi and Shin Kasahara(Dept. of Env. Sci., Miyagi Univ.)

O-16
A. oryzae ^~íͥɿǔĂ̷đҾ¤Ü®´åͥɿƂyαƣʒβΣ~̫ӵҧ̅ô͕ ǹĊնϝŚ żçԋն͹Ө ӓĂ մՉȮϕǫä҆յ ΫǏx|ҎqǪˆ~МΥѺ§ÂÔҧҸ~Ь̈նǼ~[ѺՖ~§ÂÔë|Պɉ|Ǫb~Ă̷đҾβΣαƣʒԄĘǼ¤Ü®´åմĒìն¤Ü®´åyҬӭյ_ǾǏjwZfy_Ũ˘jqQh|ն¼Üâ®
¤ÝË¼åÔҧ̅~Ь̈^նǫԌŤ~¤Ü®´å_̨ɗ|íͥɿƂhw]նӳɉ~ϟϹȋ|]dǗ՜˿ĔìxψΫnmAϗtwZBfy_˘^y{tqQψғяն̬R{МΥѺϵγZwնo~[{íͥɿǔ¤Ü®´åĊΕϋ|ͥɿƂhnնˍҡƂƣΣ~ƚɰұwZQ˷ψғxն
Aspergillus oryzae ^ͥɿƂhnq¤Ü®´åyնo^ƣʒhqˍҡƂƣΣ~̫ӵ͑ȉ|uZwǝƭlQ
 A. oryzae |]dӞŞψΫËàÉÝâ¥»å´|ǘv`նíͥɿǔ¤Ü®´åԃũjqQo~ô~պu~¤Ü®´å|ԫjwնÑÝ¦´½ƣʒԒУմPKSյ¨å½lԄĘǼ~ӪĿ| Zn Éâ¡åˮl DNA Ьƣɿ´âÅ¤Ӕ¨å½lԄĘǼ_ǾǏjwZqQo~ӞŞǆǼ̬´âÅ¤Ӕ¨å½lԄĘǼ amyB ËàÖå´åūɱìxҵȞψΫlɦӔӞʴĢĥʒjqЬ̈նPKS ƪɥҲ¤Ü®´åŚ~қˆԄĘǼ~ӞŞψΫ_ͥɿƂjqQh|նf~ɦӔӞʴĢxնջϵՖ~ƂƣΣ_ՕҀ|βαhfyψҠjqQƢϵ NMR ]ЙȒӔԚŤ̅~Ь̈նo~ƂȀ̫ӵ͑ȉjqQoնƥçÑÝ¦´½դ̖^ƣʒhqնopξ{Õ¼¢¬ҵȞĢxYtqQĒë|ŷ\w˷ψғxն¤Ü®´åŚ|ǾǏlĵ՛ԒУγZq in vitro Ƙɼ~Ь̈^նfƂƣΣ~αƣʒЫӚ|ԫjwӀһlQ
Activation of a silent secondary metabolic gene cluster by upregulating a transcriptional regulator in A. oryzae
Takehito Nakazawa, Kan’ichiro Ishiuchi, Kenji Watanabe(School of Pharmaceutical Sciences,Univ. of Shizuoka)

33

O-17
®¤àå® Fusarium asiaticum ~¼Ý¨º°âН͇УβαҵȞlȺë ՕP*ôȴ ԶPɌœԘçù մΦ͜ü͓ӧϟä*՘ƳȄŒǸƵėյ
Fusarium asiaticum Áϯ̤Σ~ԩѭ˳Ē԰~Ϸ|ʎ̍jwӕ^ςɟ`ӗfjPĊλ|ˮȍ{¼Ý¨º°âН͇Уմ¿ÄßÂåÞä»¢¬¿ÄßÂåÞյ҂ϸl_Po~͇УβαҵȞ̴̫˘^x{ZQofx˷ϟϹxP̤Σ|ɑbƪКy͇Уβαy~ԫİ|uZw̥ҫjqQ
ŤՂǑ~ξ{ F. asiaticum10 Ѻ̓īұjP¥Þ¨å®PÉÞ¤¼å®P®¤àå®P1-¦®¼å®P¿®¼å®PÓàÎ¤¶âPÓàå®PÒÞ¼å®P°ÞàÇå®P¢¬àå®opΓУͽyjwʹŷjwͭĢǗ՜jqQo~Ь̈PŒw~Ѻ̓xP®¤àå®P1-¦®¼å®ƕ¿®¼å®|͇Уβα_ɤbҵȞhqQ̷|®¤àå®~̫ӵξɿĢxYÅÜ¶Âå®PÒÞ¹àå®P¹ÜÂå®ΓУͽyjwǗ՜jqyfPѺĢαіԚ®¤àå®γZqǞƣy ƥ̬xYtq_P͇УβαҵȞh{^tqQ1-¦®¼å®ƕ¿®¼å®®¤àå®|ÉÞ¤¼å®_=2,1-ЬƣjqÝ©КxY
fy^PF. asiaticum ~͇УβαҵȞ|P®¤àå®̫ӵ_ԘҞxYfy_ʱȖhqQh|P®¤àå®yÒÞ¼å®opΓУͽyjwǗ՜jP͇УƣʒԒУԄĘǼ~ψΫԚЫ˞ϋ|ҧ̅jqQ
o~Ь̈P®¤àå®ΓУͽyjwǗ՜jqǞƣ|~ƣʒԒУԄĘǼ~ψΫԚ_ǣŷjqQĒë^P
F. asiaticum xPʎ̍jq̤Σ|ƪ®¤àå®_͇УƣʒԄĘǼ~ψΫǣŷhnP͇УβαҵȞlfy_ϥƷhqQ
Effects of carbon sources on trichothecene production and Tri gene expression by Fusarium asiaticum.
Akira Kawakami, *Takashi Nakajima, and Kazuyuki Hirayae (Kyushu Okinawa Agricultural Research Center, *Food
Safety Commission)

O-18
laeA ξϵψΫ| Cordyceps militaris ~Ă̷đҾͥɿƂ˴ì ͨ 1նRina Rachmawati1նąƐƟԾ 2նċɌƉý 1մ1 ԬǫäαΣȾȀǌԻćͦ°ն2 ӧϟ̴̫ä̲̈ϟյ
VϑϋW Cordyceps militaris ˕҉|ʎ̍PȑαlԻ|Ǫ̬{αέͥɿΣӔαβlfy^PƝb^̬R{Źγˮl΅ˎ҆yjwŪγhwZМΥѺxYQӪɍP˷Ѻ^αέͥɿˮlƂƣ
Σ_ƋՂƥȉhǷwZ_Po~ˆӳɉМΥѺ_§ÂÔë|ĳˮlyĀͻhαƣʒԄĘǼˆy͈y˵rҀjbȠ{ZxYQf~ƐǆyjwPçѪϋ|МΥѺ~Ă̷đҾΣӔαβǗ՜˿Ĕ|ɤbĬǾlqPȊգȋx C. militaris _˕҉|ʎ̍lԻ~Υ͙_ŜΫx`wZ{Z^xYyю\QLaeA  Aspergillus Ȫ|]ZwĂ̷đҾ̺|ūɱl¥àåÄÞß£Øßå´åyjw̴њlfy_˘^y{twZQʓR LaeA  C. militaris ŚxξϵψΫhnfy|PfxψΫj
wZ{^tqĂ̷đҾΣӔαƣʒԄĘǼ~ψΫͥɿƂҎZPˍҡƂƣΣ~ψҠäƥȉ|u{efyϑʨ
jqQVˎ͜ƕЬ̈W ˷ϟϹx LaeA yjw~̴њ_˘^y{twZPA. nidulans ὲ~ laeA ԄĘǼ
Beauveria bassiana ὲ~ gpd ËàÖå´å|ЬƣjPËà¼ËÜ®¼ PEG ͜| C. militaris |ȞőjqQ
ɰqɦӔӞʴĢ|uZwƢϵǗ՜˿Ĕ̥ҫjPαβhqĂ̷đҾΣӔ|uZwӰϓ HPLC |Ť
̅jqyfPսu~Ǘ՜˿ĔxŌ~Ѻ̓xҠ{ZÈå¤~ţΫ_ҥͻhqQo~Èå¤~[sջu|uZwЙҚҎZP1H-NMRPEI-MS ҧ̅ҎtqyfPZmÎË¶½ɿ~ʞѺΣӔxY
Beauvericin ՖоĢxYfy_ϥƷhqQΫǏPo~ҳЦ{̫ӵҧ̅ôxYQ
Activation of secondary metabolism of Cordyceps militaris by heterologous expression of global regulator
Hiroshi Kinoshita1, Rina Rachmawati1, Fumio Ihara2, Takuya Nihira1(1ICBiotech, Osaka Univ, 2NIFTS)

34

O-19 (P-43)
ԄĘǼǪԘϠǥ| Aspergillus oryzae ~ Hydrophobin щ~̴њҧ̅ȬȺ Ьն͋ԙġ՟նôȴ˛Хմ˘͘ǫäӧäӧƂյHydrophobinմÃ½àÉåÇâյyϺ͊ô|ѺМěԧhnǼǄѺäʤǼѺՖ|Цјғȫ´âÅ¤ӔxYQĞŤǼԚմ70~150a.a.յPòҤǻɿyZ[ΤɶϥlQÖÂÒåyjwѺĢǩ|Ť͛hqɮPҤ͋-ο͋ιՋ|]ZwѥɁՀƣjPѺМŤαǼ~ʹ͋ɿ~ĐîP̤ΣЦјғՋ{z~ο͋Ջ
~ƫϘ|ԫîlfy_ϛwZQçѪ|Pպu~Ѻ̓ăZ|ϓƥɿ~ĞZ Hydrophobin ԄĘǼқˆʖˮjwZ_Po~ĨZŤd|uZw yzҧ˘hwZ{ZQ˷ϟϹȋxլѺ Aspergillus oryzae |͟ϑjPHydrophobin ¨å½lyʱȉhԄĘǼ 4 uƋՂj
մhypA,B,C,DյPhypA,B,C |uZwψΫϢҴjwZQҋŏҌƣ´âÅ¤ӔγZqȥǏҧ̅|P
ŤαǼ| HypA j^ҥȖhmPçˎѺМx HypA,B,C ~Œw_ȥǏlfy_ϥhqQHypA y
HypB,C ~Ԫ~ȥǏɿ~ɀξ^ƥçѺ̓_ψΫl Hydrophobin ~̴њϋĨZŤd_ϥƷhwZQ˷ϟϹx hypA, B, C |uZwƋΦPĂԘ]êԘϠǥ̓ĥҚjնo~ғΫǔҧ̅^op~̴њҧ˘lfyϑϋyjwZQÝÞ´Ô PCR ҧ̅PɦʏҥȖPψѰΪͻȉPʹ͋ɿұգPSEM |ɴЦ̫ӵҧ̅~Ь̈PA. oryzae ~Ã½àÉåÇâщop~ψΫ˞˳]ȥǏķʖ_ξ{PoΦϾjw̴њjwZfy_ϥhqQqǪԘǧξ̓xPǗ՜˿Ĕ|ʹ͋ɿ_ϥhqfy^PӞŞԚ~ĞZ HypDPYZ Hydrophobin Ēǩ~ΣӔѺĢ~ʹ͋ɿ|ԫîlƞњɿ_ϥƷhqQ
Functional analysis of Hydrophobins by multiple gene disruption in Aspergilllus oryzae
Yui Yamakawa, Yuka mizuno, Harushi Nakajim(Dept. of Agricultural Chemistry, Univ. of Meiji)

O-20 (P-44)
͋˥ʪŻǼÒ¤àÄÜâ® (QCM) |ιՋͥɿҊϊӔ RolA yǋĢғՋԪ~ϓăĥγҧδԈɠͅ նǫՖˣǼ նëƐĽĂ նե̳ɷ նŠ̱ӏʒնˮδϴɧնԯԌ˅ʇ մ ̂ƃǫԲäαΣβ̧Ŵʒն ԐՖϟäǘϏն̂ƃǫä˵̀ϟն̂ƃǫäǪŌϟյVїˣäϑϋWլѺαŤҧɿÑÝ®ºÞxY PBSA մ Polybutylene succinate-co-adipate յƸç~ΓУͽyjwǗ՜lyն PBSA ŤҧԒУ~ cutinase CutL1 yŕ|ն hydrophobin RolA ψΫlQ
RolA ն CutL1  PBSA ғՋ|Ý¤Þå¼lāxն PBSA ŤҧŹΪë˖hnwZQçˎնhydrophobin çѪϋ|ǋĢғՋȅŒ|ҔѡjqΥʏxɤǋ|ЬƣlΤɶʧuQj^jն CutL1 ~PBSA ŤҧԮȍjwZ{Zā^ն RolA ƫϘɮ|͋ɌˎƧϱŻlƞњɿ_ϥƷhqQofxն͋ɌˎƧƞŻÕ ¿¯Ôҧ˘lΕ|ն RolA y PBSA Ԫ~ϓăĥγÕ ¿¯Ôҧ˘lāyjqQ
VЬ̈äюȖWƢ pH |]d PBSA ~ RolA ~ƫϘԚyƞŻɿҧ̅jqЬ̈նòя|ϓԫҠţn{ ^ t q Q h  | ն RolA ~ PBSA  ~ ʱ ȉ Ь ƣ Ց ǖ Ś ~ ǧ ξ Ģ մ L137S, L142S, D134A/D136A,
K130A/D134A/D136A յĥҚjնf|uZwƥ̬~ҧ̅ҎtqQo~Ь̈նԙαǔ RolA y͈ӡjwնǧξĢ PBSA ~ƫϘњ_ǧƂjq_նƞŻɿ|ǧƂ_Ҡ{^tqQf~ā^ն RolA
~ PBSA ~ƫϘyƞŻɿξ{Õ ¿¯Ô|ĬǾlΫӅxYā_ϥƷhqQh|ն RolA ~ƞŻɿ|նT PBSA ~ƂȀ̫ӵU]TΔǧξȞő|ǧƂjqĒë~ՉՅϋäο͋ϋ{ϓăĥ
γU_ǫ`bȑîlā_ʱһhqQofxΫǏնŤǼԪϓăĥγҧ̅Җц QCM ~°âªå¶¸ËғՋ|ο͋ɗǧ\qƂȀĵ՛ˏjwնo~ғՋ|Șl RolA ~ƫϘњҰĭjwZQ
1) Takahashi et al., Mol Microbiol. 57: 1780-1798 (2005)
QCM analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces
Hiroki Tanabe 1, Keiko Orui 1, Kenji Uehara 1, Toru Takahashi 2, 3, Takanari Togashi 4, Toshihiko Arita 4,
Keietsu Abe 1, 2
(1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NICHe., Tohoku Univ., 3 NRIB., 4 IMRAM., Tohoku Univ.)

35

O-21
МΥѺ Trichoderma reesei ~°ÞÜå±եαβǧξ̓~ɦʏȀϋҧ̅ɻδ͢čպնˍδшӏǼպPǫԵ̺ǼջPȟЄƐ ͷպմպԧȮʚϯǫäαΣPջеƣθňϟϹʽʶյVϑϋW°ÞÜå±եαβМΥѺxY Trichoderma reesei P°Þàå®Ƹç~ΓУͽyjwǗ՜jqy`|ǫԚ~°ÞÜå±ҵȞαβlQfx|PT. reesei ~β̧ë~ˮγɿ^PƢϵ°ÞÜå±~ԒУȀϋҼɿӔ]°ÞÜå±ԄĘǼщ~ӞŞҺЎ̴̫ôɸyjq̬R{ҧ̅_Ҏw`qQj^
j{_ T. reesei ~ɦʏȀϋ{ϛҠy°ÞÜå±~αβɿԫӶĐdqǝƭȠ{ZQofx˷ϟϹxP°ÞÜå±αβ˿Ĕì|]Zw T. reesei ~ɦʏȀϋҧ̅]ичϋԄĘǼψΫҧ̅Ҏ[fyxPT.
reesei ~°ÞÜå±αβɿ|ˍq{ϛҠŷ\fyϑϋyjwZQ
Vˎ͜yЬ̈W°ÞÜå±αβ˿Ĕ]Պαβ˿ĔìxǗ՜jqѺМÂâ¨åºâ¥]ȉՅǎ˿ĔìxӖ̐ǔՅǼՕɴԦҥȖҎtqQo~Ь̈P°ÞÜå±αβ˿Ĕìxȥʖϋ|̨ɗ|Ѣë_tqѺМ~ɦʏϥjPЦјǤë|ǫԚ~тжΥɴЦΣӔ_ǾǏlfy_˘^y{tqQf~ΣӔ_ţΫl˿ĔΤȉlq|̬R{Ǘ՜˿Ĕմ°Þàå®Ǘ՜P¥Þ¨å®Ǘ՜PՉцǗ՜PՆy[Ǘ՜յxɰqѺĢ~ՅǼՕɴԦҥȖҎtqyfPтжΥɴЦΣӔ°ÞÜå±αβ˿ĔjǗ՜ô~ѺĢ~Σέϋ{Ŭ΍|twαkfyϥƷlЬ̈_ɰqQΫǏPтжΥɴЦΣӔ~αέȀϋɫų˘^|lq|PëҬ~Ǘ՜˿Ĕìxɰq RNA γZwÒ¤àßҧ̅ӷwZ
Q
Morphological analysis of cellulolytic fungus Trichoderma reesei
Yosuke Shida1, Mikiko Nitta1, Masako Osumi2, Wataru Ogasawara1(1Nagaoka Univ. of Thecnol, 2Integr. Imaging Res Support)

O-22
Aspergillus nidulans ~ɦӔӞʴ˞|ՊϓƥˌΡʹŷlfy|´å§ºâ¥~ŹΪƂĖ҇ՈǮ 1նԛйǲ 2 մĴȻǫä1 ŒȀ˂і̴̫ն2 έȀԌ Ϋր̂ǫԲäŎЀαƱյɦӔӞʴ˞|ʹŷlÍ¤´åŤǼԪ~ϓăĥγ|twնƘɲԎŦ_ɦʒhQf~˞նϖ̔ЦјxçѪϋ|նƥˎƧƘɲԎŦ(DR)_ŊſxYնӰˎƧƘɲԎŦ(IR)_ҥȖhfyϲxYQf~έε˘^|lq|նȐö̍ѬĢyϓƥɿʧq{ZˌΡ IR yjնՊ̺бϋ(illegitimate)Ъө~Ź̈ҺqQo~Ь̈նɦӔӞʴŹΪ 25%|ĞìjqQfՊϓƥ˶ЀЬƣ(NHEJ)Н~Ԯȍ|~yю\q~xնϓƥ(homologous)Ъө|Șl IR ~Ź̈Һqyfն´å§ºâ¥Ւɗ_
5-10 ĸë˖jqQf~[{Ź̈ȘΛyjqƋԚĢŤǼ~ʹŷxҴq_նIR ~ˎ_ë˖Ϊե
^tqQ˷͜~ɼγю\նϓƥ]ξʖϋ(ectopic)Ъө|twɰqɦӔӞʴĢ|]dնʹŷjqՊϓƥˌΡ~̍ѬĢ~ЪөϢҴjqQlyնՊ´å§ºâ¥НбxʹŷjqŤǼ_ 85%x̥ţhq~|Șjն´å§ºâ¥jqНбx 15%xYtqQuնɦӔӞʴ˞|ՊϓƥˌΡʹŷlrdx´å§ºâ¥Ւɗë˖jն̮ϋԌĝĒǩx~̍ѬĢ~ɪՍԱȉϋxYtqQf~Ь̈|uZwնɦӔӞʴ˞|ǫԚ|ƋԚĢ] IR yjq DNA ŤǼʹŷlfy|twնȐöЦјՀǇ~ӽƈ|]Zw NHEJ _çӽϋ|Ԯȍhqyю\qQo~q|ϓƥЪө_ՕǏƂjն´å§ºâ¥Ւɗ_ë˖jqQqPϓƥɿʧq{ZʹŷˌΡ̍ѬĢ|Ъө{^tqQ
Effective gene targeting by addition of excessive non-homologous fragments in Aspergillus nidulans
Yasuo Itoh1, Ayana Kon2 (1Sch. Gen. Ed., 2Fac. Sci. / Shinshu Univ., Present affiliation: Frontier Sci. Univ. of Tokyo)

36

O-23
МΥѺ|]dԄĘǼ̴њҧ̅~q~ŤǼαΣȀϋ¹åÞ~ԩψƐȴĲ˘նҝ˼խԗ͏մαΣϟյ§ÂÔËà­¤¼|Ǫb~МΥѺxo~§ÂÔ~ŒǡǘԎŦ_͑ȉhw], o~̷~®º¸ËyjwԄĘǼ~̴њҧ̅_ɭqwZQʓRÁZsςѺÖ»ÞαΣyjwγZ, ԄĘǼ~Вį{̴њҧ̅¹åÞմԄĘǼÂ¸¤¼γ, ψΫҺЎγ, ԄĘǼ´å§¸ºâ¥γ °¸¼]´âÅ¤
Ӕ~È¼åË´¥γ, ½Õâцʴո̶ǰγ °¸¼յ̫АjqQМΥѺx PEG ͜¥àÄ¤ºÝÔ͜γZwɦӔӞʴĢĥҚlfy^, zs~ˎ͜|Ūγţ̀ɦӔӞʴγÄ¾ÝåÍ¤´å °¸¼~ǘ˷դ̖yj, h|f~Í¤´å|ţѰԒ͆~ԃʡÒå åxY URA3 y 2µ Ori ȞőjwԒ͆¬×¼ÞÍ¤´åyjqQf|Ԓ͆ѺĢŚx~ϓƥϋЪʴ\ŪγjqÍ¤´å~̫Аyʾ
ǧ_ȏ˙|Ҏ\, h|f~ °¸¼|МΥѺ|]dϓƥϋЪʴ\Ģ~®¤Ýå¿â¥ŹΪƧë
hnq~ȾǮˏhwZQÁZsςѺ~ɦӔӞʴĢԃʡÒå å|, ҆ŰѐɿÒå åxYÃ¥àÒ¬âkÇÜÏ®, ÊÜ®¼ª­â, ÊßÒ¬â, Â¬®Ý¬âѐɿԄĘǼ̑՜Ҟ͍ɿÒå åxY PyrG
ԄĘǼγZψΫ| Aspergillus nidulans ~̫ʒϋ{ËàÖå´åxY  ËàÖå´åγZqQ
h|ҵȞНËàÖå´åyjw҆ŰҵȞɿ~~ˍq|ȊγƂjqQf~ҵȞɿËàÖå´å~ °¸¼Ƃ| Cre Ъʴ\ԒУ~ψΫҵȞ_ƞњ|{, Cre-loxP Н_ϢϾhqQ˷ψғx, ̫Аhq°¸¼yy|ȊγĩħnwШčlQ
Comprehensive toolkits for fungal omicses: Versatile modules for gene manipulation regarding replacement,
expression, targeting, and tagging.
Toshiaki Harashima, Marie Nishimura(NIAS)

O-24 (P-15)
ȊγլѺ̓~§ÂÔӷƂyƢԖӵβ̧~ԂɼĖ҇ Ȱ 1,2նǶȣʆɌ 1,2ն΋˼ʁǼ 2նɵȮ˗ˉ 2նϣӂ˄Ǽ 2նȬδ ĵ 2նȯìƲҘ 1, 2 մ1 ɑȴǫԲäŎЀϟն2 Ԑкϟյ
VϑϋW͵Ԑԕ͗yZtqƢԖӵҚƳ~αβΫǞxնƢҚƳ~Қӵ|ԂjqǪ̬{Ѻ̓մȊγլѺ̓յ_Ĩγhw]նf~ԃʠ~̽ƟնլѺľ^yҚƳҚӵ|ԂjqΤɿʧu[§ÂÔ_ӷƂjq~_ԃʠhẁqyю\Qfx|ʓRնȊγլѺ̓ 55 ̓|uZw§ÂÔßʉǝ|ǘvbНбҧ̅ҎZնf~Ѻ̓_ 9 u~ö{Нб|¥ÞåËƂhնƢНбyo~β̧γӲ|ԫӶ_Yfy˘^|jqQofx˷ϟϹxնßҧ̅Ҏtq 55 ̓|uZwҚլƕ͵ԐԖӵҎZնo~ԖӵΤɿ|uZwичϋ|ҧ̅jն§ÂÔßʉǝy~ϓԫ|uZwҧ̅lfyϑϋyjqQVˎ͜äЬ̈WmնƢѺ̓ЙД̼ƣ 40%~Ȭδԡ<ƂД(A40Y)y 60%~ȬδԡҁД(S60Y)γZwնopξ{Ǘ՜˿Ĕ|]ZwαіhnqДլ~ĥҚҎZն͵ԐҚӵ|ԘҞ{ɫų̈qlДլô~ԒУͥɿƕѺĢԚͻȉjqQo~Ь̈ն¥Þ¨ÓÜå±kyjqƢԒУŵĭyƢНбy~Ԫ|ϓԫ_ҠqQqնA40Y ˿Ĕy S60Y ˿Ĕxնͥɿĺo~~|ԀZ_Ҡq_նŒĢϋ{ŅƧ|uZw ƥ̬~͈ΪϥjqQ̷|ƢѺ̓|ĥʒjqДլγZwȟҡ̯~͵ԐҚӵҎZն͵
ԐԖӵ|ԫlΤɿ~ҧ̅ҎtqQo~Ь̈նψԒЫӽαʒԐ~՟͊ʒŤЇxƢНбyo~ĺ|ϓԫ_ҠqQh|նf~»å´ǘ|ǪǧԚҧ̅ҎtqyfնƢѺ̓§ÂÔßʉǝ|ǘvbНбҧ̅y çѧlЬ̈ϥjqQĒë~Ь̈^նլѺ~§ÂÔ̫ӵyԖӵë~Τɿ|ǫ`{ԫӶ_Yfy_˘ϙ|ϥhqQ
Evolution of industrial Aspergillus oryzae genome and its adaptation to the fermentation industries
Gaku Ito1, 2, Yuhei Senoo1, 2, Tuneko Sawamura2, Masahumi Tokuoka2, Atsuko Isogai2, Osamu Yamada2, Kazuhiro
Iwashita1, 2(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB)

37

O-25
Ò´¦Ö»Þyjq¢Â¨αі̴̫ҧ˘~§ÂÓ¤®Ëàå¶Ķ̳˄ 1)նĠ҇ϖù 1)2)3)նҝǙёê 1)ն̢҇ˉƻ 2)3) ( 1)ՄǌZqdն2)ÃÉ­Á¬®ն3)̂ĉȎˀǫ )՘γ¢Â¨~ǼȊĢαі̴̫~ҧ˘ϑϋyjwնfx|Ò´¦̗ǗȾϳŒǖ~ EST »å´ƚɰj»å´Íå®ƂjwZQf~ EST »å´^Ò¤àßĥҚjն̗ǗȾϳ|]dԄĘǼ~ψΫʩŻҧ̅jqЬ̈նՕҀ|ψΫǧŻlԄĘǼƐǘɦʒ˳x 56 ķնǼȊĢαі˳x 187 ķҠţjqQô| Gf.HSP9 ~[|Ǘ՜˳Ԫô~ψΫԚyǼȊĢƙԚ|ϓԫ_ҠնǼȊĢαіy~ԫӶ_ϥƷhԄĘǼ_YtqQqնǼȊĢαіíѫϥlǧξĢγZqÒ¤àßҧ̅~Ь̈նӞŞǆǼyjwϛwZ C2H2 ǔ zinc finger ҊϊӔԄĘǼ_ǧξĢxՕҀ|եψΫxYtqfy^նf~ҊϊӔ_ǼȊĢαі|ɹՐ{ԄĘǼ~ӞŞūɱǆǼxYƞњɿ_ϥƷhqQf~[|ҠţhqԄĘǼ_ȊԻ|z~[{̴њʧu~^ҧ̅lq| EST »å´rdx{bնÂâ¨å»â¥Ցǖ~ʉǝíƞ̶xYQofxնÒ´¦~§ÂÔҧ̅̷ðđ¬å¦âªåxY GS FLX (Roche) ƕ
GAIIx (Illumina) γZwȊˏjqQ2 u~¬å¦âªå^ɰqԎŦ»å´ͳƣ¸°âÊÞlfyxնԧh 33.8 Mbն®¢×ÏåÞ½ˆ_ 280 ~½ÜÉ¼§ÂÔ»å´ƚɰjqQf~§ÂÔ»å´^ԄĘǼĀͻҎ[yĀͻԄĘǼˆ 10,500 ϳɗxYtq_նEST »å´Ò¸Èâ¥lfyxĀͻԄĘǼˆ 16,097 |ѦtqQf~[|Йɗ~եZԄĘǼĀͻЬ̈ɰq|նEST »å´Đŷlfy_ԘҞxYyю\qQČɮ§ÂÔ»å´γZwԄĘǼ´å§ºâ¥Ї|ҳЦ{̴њҧ̅ӷfyxնǼȊĢαі̴̫~ҧ˘|у_twZbyю\wZQ
Genomics approach for exploring mechanisms of fruiting body development using Grifola frondosa as model
mushroom
Atsushi Kurahashi1), Masayuki Sato1)2)3), Kozo Nishibori1), Fumihiro Fujimori2)3)( 1)Yukiguni Maitake Co. Ltd., 2)Hyphagenesis Inc., 3)Tokyo Kasei Univ. )O-26
ǑҒՖ~ŕαѺ~§ÂÔҧ̅ƐŏĂԋնĠ҇^նȟȳ̺ƟնȬ˷ǴƲ մϮδϕǫäαΣӒͽյǑҒՖϖѺՖմö|ǼǃѺՖyçԌʤǼѺՖյy҈Ֆմл҈Ֆy¬ÂÄ¤ºÝյy~ŕααΣxYնǑҒʒŤyưΤˮ~Ă̷đҾβΣƪˮlQo~ɫųyjwնŕαԫİĳuq~ѺՖ~҈Ֆ|ȘlūɱΣӔyjwնYZßàÅ¬åΣӔɺԆΣӔyjw~ɫų_ю\wZQƅ҆¬å¯ԩψ|Ƨdwhi{αΣͥɿ®¤Ýå¿â¥_fx|Ҏw]նʓR Myelochroa
aurulenta մ¨¾¶¢ÕÂ¢©¦յ_βαlÏÅâǔ¼ÝºÞÎâƂƣΣ_ʞџτͥɿˮlfy˘^|jqQӪɍն¬å¦â¬â¥ʚҏ~ψȩ|ĚZնǑҒՖ|]Zw§ÂÔҧ̅_Ҏ[|{նǑҒʒŤ~αƣʒԄĘǼ~ҧ˘_˳ɭhQČǅնM. aurulenta ^ŤՂjqǑҒѺǗ՜̓γZwն§ÂÔŒĢ]¼ÝºÞÎâαƣʒЫӚ~ԫӶԄĘǼҧ̅jqQ
Genome Analyzerմillumina ϧյ|ǡǘԎŦ͑ȉjն2 ǅ~°âÊÞŢέ~Ь̈նкǡǘˆП
37.5 MbpնGC ƪԚ 47.5%xYtqQӪо~ Aspergillus spp.yкǡǘˆ GC ƪԚ|ǫ`{ԀZ{^tqQAugustus ] Blast2GO |ԄĘǼՑǖĀͻyÂºå¬Úâ|նкԄĘǼˆ 10,722ķyĀʊhն¼ÝºÞÎâƂƣΣ~αƣʒ~ŧ˳|ԫyю\ΰƂԒУԄĘǼնȠ{by 3ķǾǏlfy_˘^y{tqQ80 ķĒë~ÑÝ¦´½ƣʒԒУմPKSյԄĘǼ_§ÂÔô|ǾǏjնĂ̷đҾ¤Ü®´åyю\ՑǖҠu^tqQ
Genome sequencing and analysis of lichenized fungus
Kojiro Hara, Hikari Sato, Masashi Komine, Yoshikazu Yamamoto

38(Akita Pref. Univ.)

P-1
Aspergillus awamori ~ ligD ԄĘǼϠǥ|եՒɗϓƥЪʴ\Ȑö~ԩψե̳ɷ 1, ͋ӂ͘ 1, ϊϝ͢Ɍ 2, Ȭδĵ 1մ1 Ԑкϟ, 2 Ç¸¤յկ լ Ѻ Aspergillus awamori  ͝ ώ  Ι ԏ ~ Қ ӵ | Ĩ γ h  w Z  Ԗ ӵ ɴ α Σ x Y  Q 2008 ɍ |A.awamori NBRC4314 ̓~§ÂÔҧ̅_ȅÿjԄĘǼ~¤àå¿â¥_ȏ˙y{tqQj^jPoԄĘǼ~̴њʛʵlq|ȐöäψΫН~ԩψ_ɹՐy{Q˷ϟϹxՊϓƥЪʴ\|ԫîjwZligD ԄĘǼϠǥlfy|PեՒɗϓƥЪʴ\Ȑö~ԩψ|ʒŶjqqPf|uZwǝƭlQ[ˎ͜äЬ̈] A.awamori NBRC4314 ̓~§ÂÔ»å´Íå®^ A.oryzae AoligD ԄĘǼyϓƥɿϥl
AaligD ԄĘǼҠţjPf~ԄĘǼ¥àÄ¤ºÝÔ͜|цʴϠǥjqQ̷|PϓƥЪʴ\ŹΪ_ƧëjwZfyϢҴlq|PŤαǼ~ϘѬ|ԫîjwZyʱͻh pksP ԄĘǼ´å§¸¼yjԄĘǼϠǥҎtqQo~Ь̈PpksP ԄĘǼ~Ϡǥ|Ĩγl 5’ ƕ 3’ Ցǖ~ԧh_ 500bpĒëxY 100% ~ŹΪx pksP ԄĘǼϠǥhfy_˘^y{tqQ
Development of an efficient gene-targeting system in Aspergillus awamori by deletion of the non-homologous end
joining system
Toru Takahashi1, Osamu Mizutani1, Yohei Shiraishi2, Osamu Yamada1(1NRIB, 2Bio'c Co. Ltd)

P-2
Cre-loxP ¬®ºÔγZqլѺ|]dǪԘԄĘǼȞő¬®ºÔ~ԩψ͏Ɛϒ̲ն͋ӂ͘*նˍӂȡɠնĄƯžýմ̂ƃǫԲӧä*ԐкϟյլѺմAspergillus oryzaeյնo~Ŋq´âÅ¤ӔαβњնŤ͛њ{z^նǩ̀´âÅ¤Ӕեαβhnq~ȐöyjwŪγhwZQh|ӪɍxնМΥѺ~Ǫ̬{Ă̷đҾƂƣΣαβњ|Ϙϑjն
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Development of multiple gene expression system using Cre-loxP-mediated marker recycling in Aspergillus oryzae.
Naoki Ebara, Osamu Mizutani*, Takahiro Shintani, Katuya Gomi
(Grad. Sch. Agric. Sci., Tohoku Univ., *NRIB)

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Transformation by complementation of a uracil auxotroph of the rice straw degrading basidiomycete Cyathus
stercoreus (bird's nest fungi)
1

Kenji Yamagishi, 1Toshiyuki Kimura, 2Takashi Watanabe (1National Agricultural Research Center, 2Kyoto Univ.)P-4
МΥѺ Coleophoma empetri F-11899 |]dջϵ~̷ðđ¬å¦âªåγZq§ÂÔ°âÊÞԎŦ~͈ӡôӂƲýնȬδԿĊնфԙԴčնϣӆ͠ɠն̳˷̺͘ մȓȬϕǫäαΣȾյМΥѺ Coleophoma empetri F-11899 նͰǏɿϖѺσ͘υ҆ micafungin ~ƐĢxY FR901379 αβlQ˷ϟϹxնFR901379 αƣʒЫӚ~ҧ˘ϑϋyjwն2 ϵ~̷ðđ¬å¦âªågenome sequencer
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GenomeMacher γZҢңϋ|͈ӡjqQo~Ь̈նGAIIնFLX ~ƋΦ°âÊÞԎŦ çѧjwZqQComparison of genome sequence assembly by two NGSs in fungus Coleophoma empetri F-11899.
Kazuya Nakaya, Masato Yamada, Yohsuke Orino, Yasuhiro Isogai, Seiji Hashimoto(Dept. of Biotechnol., Toyama Pref. Univ.)

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Kiyohiko Igarashi1, Chiaki Hori1, Masahiro Samejima1, Yasuo Uemura2, Aya K. Takeda2(1Dept. of Biomaterial and Science, Univ. of Tokyo, 2Genaris, Inc.)

P-6
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Functional genomic analysis of subtropical fungi harboring antibacterial activity
Tomomi Toda, Yoshinori Koyama, Myco Umemura, Hideaki Koike, Masayuki Machida(Bioproduction RI, AIST)

41

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ϊլѺ Aspergillus kawachii IFO 4308 ̓~§ÂÔҧ̅ĂϪ͠ǘ 1ն̢ç̲ 1նȬìɨǨ 1նƲδ̺ǭԋ 2ն̠Ɛɚƌ 2նեìϭ˛ 2նǫ̢Ĳԋ 2նЂȺ҅ 1նδđɚč 1ն
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Genome analysis of white Koji mold Aspergillus kawachii IFO 4308
Taiki Futagami1, Kazuki Mori1, Ayaka Yamashita1, Shotaro Wada2, Yasuhiro Kajiwara2, Hideharu Takashita2, Toshiro
Omori2, Kaoru Takegawa1, Kosuke Tashiro1, Satoru Kuhara1, and Masatoshi Goto1(1 Faculty of Agriculture, Kyushu Univ., 2 Sanwa Shurui, Co. Ltd.)

P-8
SOLiD3 |լѺ~ RNA-seq yլѺ͈ӡ§ÂÔ»å´Íå®~˪ˍȯìƲҘ 1նȴƐ˘Ǽ 1, ë˼͠ǯ 2, Ȭδ ĵ 1 մ1 Ԑкϟ, 2 ̓ɞėϧ­¾Ý®յ
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RNA-seq analysis of Aspergillus oryzae and update of Comparative Fungal Genome Database
Kazuhiro Iwashita1, Akiko Shimahara1, Yasuo Uemura2, Osamu Yamada1 (պ NRIB, 2 Genaris, Inc.)

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EGFR-VHH ¨å½lԄĘǼնլѺὲ´ ÓÜå± A ~¬¥¾ÞÎË¶½(sTAA)ԄĘǼ~ìͦ|ӶЬjqQ̫АjqԄĘǼլѺ~§ÂÔô|ЪөնsodM ËàÖå´ūɱìxψΫhnqQGPY ͭĢǗǑôն30 Dն150 rpm x 10 ˒ԪǗ՜ҎZնʞ EGFR-VHH ~Ť͛αβԚ~Ы˞ǧƂ®´âÊà¸¼
͜|ҧ̅jqQEGFR ЬƣњնELISA ͜]Æ¼ʗɌëόφ A431 Цј~Ьƣɿ|ҰĭjqQVЬ̈WǗǑô~ʞ EGFR-VHH ~Ť͛αβԚП 80 mg/L |ӿjqQǗ՜ë͵ô^ЙҚjqʞ EGFRVHH ն§Þΐӽ¤àÒ¼¥ÜÉåxƋç~Èå¤ϥjնVHH |Τˮ~ԁХǩśĻŏĂѬɿմCDյ®Î
¤¼Þϥjqfy^նėƣĢɦʒjw]mն̺jZ̫ӵˮlā_ʱȖhqQqնКԥĐŷҴmնEGFR Τξϋ{Ьƣњϥjնh| EGFR եψΫjwZ A431 Цј~ЬƣɿˮjqQĒë~Ь̈^նЪʴ\լѺ|̴њˮl VHH _Ť͛αβhqfy_˘^y{tqQ
Production of camel variable heavy chain antibody fragment VHH by Aspergillus oryzae
Jun-ichi AOKI1, Soichiro TABUCHI1, Fumiyoshi OKAZAKI2, Chiaki OGINO1, Tsutomu TANAKA2, Hiromoto
HISADA3, Yoji HATA3, Akihiko KONDO1 (1Dept. Chem. Sci. Eng., Grad. Sch. Eng., Kobe Univ., 2Org. Adv. Sci.
Tech., Kobe Univ., 3Res. Inst., Gekkeikan Sake Co.)

P-10
լѺ A. oryzae |]då¼É­åūɱ|ξϵ´âÅ¤Ӕαβɿ~ƧëѹԪԶɻ 1նȢ Ӣȃ 2նõȬΉç 1նƃ˷žf 1 մ1 ̂ǫԲäӧαϯն2 ƻ˘ǫȀ̒ä҆յ
VϑϋWå¼É­åϖ̔αΣ|ɑbĳǾhqЦјŚŤҧ̴̫xYն̑՜ՙ՝˞~αǾʔμyjw
̴њl ^նψαŤƂնŐπɼЉնЦј̾{z|ԘҞ{ɫų̈qjwZQʓRĒŮնA. oryzae|]Zw S-S Ьƣ̶ʷjqǧξǔ<?ÓÜå±ψΫhny, å¼É­åĬǾϋ|ͭјƚөfy˘^|jq պյQfնå¼É­å_ξϵ´âÅ¤Ӕαβ|]dÐ¼ÞÁ¸¤~yuxYfyϥƷjwZQ˷ϟϹնA. oryzae |ξϵ´âÅ¤Ӕ~ŹΪϋ{αβϑϋyjն
å¼É­å~ūɱ|ξϵ´âÅ¤ӔեŤ͛αβ̓~іϵҎtqQVˎ͜äЬ̈Wå¼É­å~ҵȞ|ԫîl Aoatg1 ] Aoatg13նå¼É©²åÔ~ɦʒ|ɹՐ{ Aoatg4 ] Aoatg8նå¼É­¸¤Ð»ȷǥ|ɹՐ{ Aoatg15նĒë 5 u~å¼É­åԫӶԄĘǼ|uZwϠǥ̓ĥҚjqQop~ԄĘǼϠǥ̓xξϵ´âÅ¤Ӕ~Ö»Þyjw¬ä¢Ö¬âψΫjնǗǑô~αβԚͻȉjqQo~Ь̈նAoatg15 Ēǩ~ 4 u~å¼É­åԫӶԄĘǼ~Ϡǥ̓|]ZwˬեxП 3 ĸ~¢Ö¬âαβԚ~ǣŷ_ҠqQj^jնå¼É­å̶ʷ̓ӳɉ~Ǘ
Ǒx~ǣ́ԙἀyɀ{Z~~նŤαǼɦʒ_ԮȍhΤɶˮjwZQΫǏնå¼É­åԫӶԄĘǼψΫʜūƞњ{ËàÖå´åմthiA ËàÖå´åյìxψΫhnնŤαǼɦʒ˞|ψΫ
â|jն¬ä¢Ö¬âαβ˞É|lfy_ƞњ{̓ĥҚjնǗǑô~αβԚ̥ҫjwZQ
1յKimura et al. (2011)Biochem. Biophys. Res. Commun., 406: 464-470
Highly efficient production of heterologous proteins by control of autophagy in Aspergillus oryzae
Takashi Kikuma1, Jaewoo Yoon2, Jun-ichi Maruyama1, Katsuhiko Kitamoto1(1 Dept. of Biotechnol., Univ. of Tokyo, 2 College of Pharmacy, Keimyung Univ.)

43

P-11
լѺ A. oryzae ὲ¢¬Ü¾å±~ичϋ¤àå¿â¥yψΫαβøδƌŌ 1ն͞ԌʇǼ 1նǡδƲŶ 1նǒ̂ɠ̲ 1նǛͨǼ 1նϝδƌ̲ 1նϰ͢Ă 1նӪ҇˝ɧ 2ն̤δōш 3
մ1 ˭̘şäкϟն2 ϪʕǫԲäȾäɼƂն3 ĉǫԲäӧäɼγαƱյ
VϑϋW՘ˋyЁƣj{ZՊƞ՘ɿÄÒ®^ŮŢέäԒУŢέäɴαΣψԒ|Ä´ÂåÞαβlāʧдɿϧė~ȊΫ|ytwԘҞxYշɃӌ°ÞÜå±Ű~x°Þàå®ŹΪbƞͿɿ°àÝ©К|xŤҧx`{Zā_ǪZշfxʓRӞŞǆǼ AoxlnR ԄĘǼեψΫլѺ~Ǘ՜ͭë͵ɃӌԒУŰ|ʹŷlfy|նŮŢέ϶áÜ~ԒУŤҧ_ıӷhā˘^|jw`qշofx˷Ǘ՜ͭë͵|ƪԒУщ~[sԣy{ԒУƥȉlāϑϋyjqշVˎ͜WAoxlnR ԄĘǼեψΫ̓~Ǘ՜ë͵ 1 ̷ŌՅ͊͡ŻxŤՂɮնöҞ{Äâ½|uZw LC-MS/MS Ť̅]Ò®¨¸¼̥ФҎtqշţΫՒɗ~եZԒУщ|uZwնCAZy database ^ичϋ|²«Ô¨å½lԄĘǼщ~ʣţҎtqշɰqʉǝ^ƢԄĘǼ~ψΫ¨â®¼Ü¤¼ĥҚjնլѺ A. oryzae
OSI1013 ̓|ȞőjqշVЬ̈WLC-MS/MS Ť̅]Ò®¨¸¼̥Ф~Ь̈ն¢¬Ü¾å±щ_ǪbψΫ
αβjwZā_˘^y{tqշ»å´Íå®̥ФjqЬ̈նˑϛ~¢¬Ü¾å±ԄĘǼ_ 4 ϵՖնʱȉԄĘǼ_ 4 ϵՖǾǏlfy_˘^y{tqշf 8 ϵՖ~ԄĘǼ~ψΫН̫АjնopɦӔӞʴĢɰqշƢǗ՜ë͵ SDS-PAGE |ϢҴjqЬ̈նŒw~ϑϋ´âÅ¤Ӕ_αβhwZā_˘^y{tqշɐu^~ʱȉԄĘǼ~´âÅ¤Ӕ|¢¬Ü¾å±ͥɿ_ҠţhqշVҾӦW˷ϟϹ NEDO ÄÒ®ÁÞ£åЇեŹΪӞʴʚҏԩψ~çΰyjwҎq~xYշ
Expression of xylanase genes from Aspergillus oryzae.
Hiromoto Hisada1, Etsuko Habe1, Kazunori Shiota1, Hiroki Bando1, Hiroko Tsutsumi1, Hiroki Ishida1, Yoji Hata1,
Akihiko Kondo2, Mitsuyoshi Ueda3 (1Res. Inst., Gekkeikan Sake Co., 2Dept. Chem. Sci. Eng., Kobe Univ., 3Div. Appl.
Life Sci., Kyoto Univ.)P-12¤âԓαβМΥѺ Aspergillus niger |]d oxaloacetate hydrolase ԄĘǼ(oahA)~ϠǥyեψΫ|¬ØԓαβЫӚ~̥үȟ̇ʐçն˷δҘ̲ն˰ԌӏΊն̚˼ŏǭԋ մ˔ǫäέȾäɼƂյ
VϑϋWМΥѺ Aspergillus niger |¤âԓαβ|]d¬Øԓ~űαն¤âԓαβԚ~ĞìЙҚȾϳ~ΜՁƂ{z~Ɛǆy{Q˷ϟϹxնA. niger |]d¬ØԓαʒЫӚ~̥үϑϋyjwնA. niger WU-2223L ὲ oxaloacetate hydrolase(OAH) ԄĘǼ oahA ¤àå¿â¥jqQh| oahA Ϡǥ̓yեψΫ̓ĥҚjն¤âԓαβԚy¬ØԓαβԚ|uZw̥ҫjqQVˎ͜]Ь̈W¤âԓαβМΥѺ A. niger WU-2223L ὲ oahA  2 u~â¼àâƪŒԧ
1,232bp ~ԄĘǼՑǖ^ʒն341 ķ~ÓÂԓ̀ǘ¨å½jwZqQoahA Ϡǥ̓(DOAH-1) oahA ~çԌ̶ǰhnq oahA ϠǥγËÜ®Ó½γZwն WU-2223L ȐöyjqËà¼ËÜ®¼-PEG ͜|ĥҚjqQDOAH-1 |]Zw oahA ~ӞŞ] OAH ~ͥɿ_ͬǰjն¤âԓαβ˿Ĕ|]Zw¬Øԓαβj{ZfyϢҴjqQoahA եψΫ̓(EOAH-1)МΥѺγեψΫËàÖå´åP-No8142 ìͦ| oahA ӶЬhnq̫ӵ~ËÜ®Ó½γZwն WU-2223L ȐöyjqËà¼ËÜ®¼-PEG ͜|ĥҚjqQEOAH-1 |]ZwնoahA ~ӞŞԚyy| OAH ͈ͥɿ_ˬǫ 35 ĸxǣŷjնΓУͽyj
w 30 g/l ~¥Þ¨å®ƪǗǑx~ 12 ˒ԪǗ՜|w¬ØԓαβԚ_ 28.9 g/l(ƙΪ 96%)|ӿjqQĒë
նA. niger WU-2223L |]d¬Øԓαβ_ OAH |¢ªàԑԓ~ŷ͋Ťҧ|ὲl~xն
oahA եψΫ|¤âԓ^¬Øԓ~ψԒӞʴ_ƞњ{fy˘^|jqQ
Disruption and overexpression of oxaloacetate hydrolase gene (oahA) in citric acid-producing Aspergillus niger
Keiichi Kobayashi, Yuki Honda, Takasumi Hattori, Kohtaro Kirimura(Dept. Appl. Chem., Fac. Sci. Eng., Waseda Univ.)

44

P-13
»âËâӔԳƒȟժÉ®ÒγZqլѺψԒx~ƞͿƂ|ԫlϟϹԜ˴˟ նƲδϖĊ նô˼ơ նĠԙŌ˝ նȣԫĽĂ նǫЍĴç նԛǼ˘Ҙ   ԛ͕Ⱦǫä§ÂÔϟ ˒͵ÉÞÒ[ϑϋ] ȟժ,Ɍʒ 21 ɍx 570 è¼â_՘Лγyjwͦӳjw],ȟժÉ®ÒyjwɍԪ 50 è¼âĒëʭţh, yz_՘ƳÄÒ®~ɛ̡ΣyjwʙwZQÜÇÂå®ä¢¬àå®~ƞͿƂ|,ȟժÉ®ÒǗǑyjqլѺǗ՜x,ȟժÉ®ÒŚ~»âËâ|twÓÜå±НԒУ_ҵȞhq,ÌÓ°Þàå®Ťҧ|ԫӶlԒУ~ψΫ_Լ,ËàºåÔҧ̅_ǈՃxYtqQofx˷ϟϹx,ȟժÉ®Ò|ƪwZ»âËâӔԳƒj,ˬԂ{ԒУŢέ˿Ĕx~̴њɿК~ƞͿƂƧë,]
ƞͿƂ|ԫӶlԒУ~ԄĘǼщ~ƥȉϑϋyjqQ[ˎ͜]Ь̈] ¤âԓyå¼¤ßåÊ|tw»âËâӔԳƒj,̮;~ȟժÉ®ÒǗǑմ̮; յ^ ,»â Ëâ Ӕ Գ ƒȟ ժÉ ® Ò Ǘ Ǒ մAC+Washյ_ҺҚx`fy_Ť^tqQojw ,̮;y
AC+Wash ~ǗǑx~Ò¤àÊ~ŮŢέҎ{[fy|ƞͿƂ~ǧƂҺqQ̮;~ȟժÉ®
Ò|Ò¤àÊŢέҎ{twƞͿƂΪ|ǧƂҠ{^tq_,AC+Wash ~ȟժÉ®Ò|Ò¤àÊŢέ 1 ŤԪҎ{[fyx,ˑϛ¢¬Ü¾å±ԒУ|¢¬àå®ƞͿƂΪ_ƧëjqQq,լѺǗ՜|]Zw~´âÅ¤Ӕ͈ӡjqyf,ËàºåÔҧ̅xˍҡ~ԒУ_ˆϵҠu^,DNA Ò¤àßҧ̅xψΫԚ_ 27 ĸեZˑϛ~¢¬Ü¾å±ԄĘǼ,]ˍҡ~=¥Þ ¾å±_ 3 ϵՖҠu^tqQStudy on solubilization by the Aspergillus oryzae fermentation using the starch removal wheat sliding paper-door
Akira Suzuki1, Masato Wada1, Tsukasa Nakamura1, Motoaki Sano1, Kenji Ozeki1, Siniti ohashi1,akihiro kaneko2(1KIT, 2nissin pharma)

P-14ͭĢǗǑxեαβlլѺ̴њ˵ϛ´âÅ¤Ӕմ .&յԄĘǼ~ҧ̅yŪγϝȶͨϿնИĶ͠ӣնʘƚȨ̙ǼնĠԙŌ˝նȣԫĽĂնǫЍĴç մԛ͕Ⱦǫä§ÂÔϟյ
VϑϋWլѺ! ̓γZq% ͭĢʪϐǗ՜xեαβh´âÅ¤ӔҠţjPǗ՜ŧ˳^ɮ˳xȄȉjwѺĢǩ|Ť͛αβhfyǝƭjqQČǅP˷´âÅ¤ӔԄĘǼ|]ZwÞ Ý˿ĔìxɼЉlԎŦ_Ҡu^tq~xPÞ Ý˿Ĕìx~Ǘ՜|]dԄĘǼ~ψΫԚP]αβɿ~̥ҫҎ
tqQ
Vˎ͜]Ь̈W˷´âÅ¤ӔP% մ%*&56964&(6P&(6 *3621*P/7(25*յͭĢǗǑ¥Þ¨å®?¼ÝË¼âͭĢǗǑեРɗǗǑ~ˎ_Рɗѫbαβhfy_Ť^tqQqP˷´âÅ¤
Ӕ~αβԚ_ǪZǗ՜˿ĔP»¢®¼Ýâ?%*&56*964&(6ͭĢǗǑեαβǗǑ~αβԚ_ǪZfy_Ť^tqQŒw~ǗǑ|]ZwPÞ Ý˿Ĕ|]dǗ՜~Ь̈PÞ Ýľx˷´âÅ¤ӔԄĘǼ~ψΫԚ_ǪbPq˷´âÅ¤Ӕ_եαβhfy_Ť^tqQΫǏP˷´âÅ¤ӔԄĘǼy<?ÓÜå±ԄĘǼ~ψΫԚy´âÅ¤Ӕ~҂ϸԚ͈ӡjwZQqP˷´âÅ¤Ӕ¢×Ýå´âÅ¤Ӕyj
wγZqPξϵ´âÅ¤ӔαβН~̥ҫϑʨjwZQ
Analysis of 25kDa protein gene on Aspergillus oryzae liquid clutureշ
Hiroaki Ishizaki, Taisuke Awakura, Momoko Tedoriya, Motoaki Sano, Kenji Ozeki, Shiniti Ohashi(KIT, Univ. GENOMU Labo.)45

P-15 (O-24)ȊγլѺ̓~§ÂÔӷƂyƢԖӵβ̧~ԂɼĖ҇ Ȱ 1,2նǶȣʆɌ 1,2ն΋˼ʁǼ 2նɵȮ˗ˉ 2նϣӂ˄Ǽ 2նȬδ ĵ 2նȯìƲҘ 1, 2 մ1 ɑȴǫԲäŎЀϟն2 Ԑкϟյ
VϑϋW͵Ԑԕ͗yZtqƢԖӵҚƳ~αβΫǞxնƢҚƳ~Қӵ|ԂjqǪ̬{Ѻ̓մȊγլѺ̓յ_Ĩγhw]նf~ԃʠ~̽ƟնլѺľ^yҚƳҚӵ|ԂjqΤɿʧu[§ÂÔ_ӷƂjq~_ԃʠhẁqyю\Qfx|ʓRնȊγլѺ̓ 55 ̓|uZw§ÂÔßʉǝ|ǘvbНбҧ̅ҎZնf~Ѻ̓_ 9 u~ö{Нб|¥ÞåËƂhնƢНбyo~β̧γӲ|ԫӶ_Yfy˘^|jqQofx˷ϟϹxնßҧ̅Ҏtq 55 ̓|uZwҚլƕ͵ԐԖӵҎZնo~ԖӵΤɿ|uZwичϋ|ҧ̅jն§ÂÔßʉǝy~ϓԫ|uZwҧ̅lfyϑϋyjqQVˎ͜äЬ̈WmնƢѺ̓ЙД̼ƣ 40%~Ȭδԡ<ƂД(A40Y)y 60%~ȬδԡҁД(S60Y)γZwնopξ{Ǘ՜˿Ĕ|]ZwαіhnqДլ~ĥҚҎZն͵ԐҚӵ|ԘҞ{ɫų̈qlДլô~ԒУͥɿƕѺĢԚͻȉjqQo~Ь̈ն¥Þ¨ÓÜå±kyjqƢԒУŵĭyƢНбy~Ԫ|ϓԫ_ҠqQqնA40Y ˿Ĕy S60Y ˿Ĕxնͥɿĺo~~|ԀZ_Ҡq_նŒĢϋ{ŅƧ|uZw ƥ̬~͈ΪϥjqQ̷|ƢѺ̓|ĥʒjqДլγZwȟҡ̯~͵ԐҚӵҎZն͵
ԐԖӵ|ԫlΤɿ~ҧ̅ҎtqQo~Ь̈նψԒЫӽαʒԐ~՟͊ʒŤЇxƢНбyo~ĺ|ϓԫ_ҠqQh|նf~»å´ǘ|ǪǧԚҧ̅ҎtqyfնƢѺ̓§ÂÔßʉǝ|ǘvbНбҧ̅y çѧlЬ̈ϥjqQĒë~Ь̈^նլѺ~§ÂÔ̫ӵyԖӵë~Τɿ|ǫ`{ԫӶ_Yfy_˘ϙ|ϥhqQ
Evolution of industrial Aspergillus oryzae genome and its adaptation to the fermentation industries
Gaku Ito1, 2, Yuhei Senoo1, 2, Tuneko Sawamura2, Masahumi Tokuoka2, Atsuko Isogai2, Osamu Yamada2, Kazuhiro
Iwashita1, 2(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB)

P-16
МΥѺՖxɑbĳǾhq̴њ˵ϛԄĘǼ~լѺx~ҧ̅ŠȺ ƟǼ ąõ ϒ ȗʕɻĳ ͐δ ŊέǼ ɮ̺҇Ūȯì ƲҘ  մ ɑȴǫԲäŎЀϟ Ԑкϟ üǫԲӧյլѺƕď~МΥѺ~Œ§ÂÔ¬å¦â®ҧ̅~Ь̈P50ճĒë~ԄĘǼ_̴њ˵ϛxYā_˘^y{tqQqPfx~͈ӡ§ÂÔҧ̅~Ь̈Pf~̴њ˵ϛԄĘǼ~Ǫb_̬R{МΥѺՖxĳǾhwZfy_˘^y{twZQիѺ~ҧ̅xPf~̴њ˵ϛԄĘǼДլЇxψΫjwZfy_ϢҴhqfy^Pf~ĳǾhq̴њ˵ϛԄĘǼPМΥѺ|ŕӳxǋˮ~αΣŤǼ¬®ºÔ|ԫîlā_ϥƷhqQofxМΥѺՖ|ĳǾhwZwPïuДլxեψΫjwZիѺԄĘǼ|uZ
wP301 ԄĘǼҧ̅ȘӅyjwԃʠjqQh| Uniplot |Șl BLAST ҧ̅xPȊգ|̴њҧ̅hqÏÖà¥_{ZfyϢҴjq 257 ԄĘǼ~[sÜâµÔ| 156 ԄĘǼԃʡjPϠǥ°¸¼ƚɰ

jq 147 ԄĘǼxԄĘǼϠǥұqQɰq̓xԄĘǼϠǥ~ϢҴҎtqЬ̈PÏÖäÌºàƪw 130 ԄĘǼ~ϠǥʒŶ̓ɰwZQf|uZwPɹՐyϥƷhԄĘǼPɦʏҥȖ|Τɶ
ϋ{ғΫǔϥlԄĘǼ_ҠZrhq~xǝƭlQ

Analysis of Aspergillus oryzae genes conserved among filamentous fungi but not characterized the functions.
Humiko Tomikawa 2, Nao Imaru 1. Shiho Terado 1. Yuriko Ikeda 2. Masatoshi Goto3. Kazuhiro Iwashita 1,2
մ1 AdSM,Hiroshima Univ P2NRIB P3 Kyushu Univյ

46

P-17
Дիեαβ´âÅ¤ӔԄĘǼϠǥ̓~Ԗӵë~Τɿ|uZwѭδΛ˘ 1,2նϬƐϖçԋ 1,2նì˷ՏǼ 2ն͖ԙш÷ԗ 2նȯìƲҘ 1,2նȬδĵ 2
(1 ɑȴǫԲäŎЀϟն2 Ԑкϟ)
VϑϋWɥϟϹȋ|]ZwˢӳγДիyǫƨԖγДի~ËàºåÔҧ̅_Ȋˏhն160 ķ~ԄĘǼ(Ś 1 ԄĘǼ_Áὲ)_Дի´âÅ¤ӔyjwƥȉhwZQfx|նʓR~¥ÞåËxf~ԄĘǼ~ô^ն55 ԄĘǼ|uZwϠǥ̓ĥʒjնαіɦʏɦʒ{z|uZwҧ̅Ҏtw`qQ
ojw˷ϟϹxնfx|ɰwZԄĘǼϠǥ̓γZwնϠǥjqԄĘǼ~Қիƕ͵ԐҚӵë~̴њ|uZw˘^|lāϑϋyjqQVˎ͜äЬ̈WƢԄĘǼ|u` 2 ̓Ēë~ΦϾjqԄĘǼϠǥ̓ĨγjնҚիyȟĎөұգҎZҧ
̅ҎtqQmŧ|ҚլҎZնДի|]dԄĘǼϠǥ̓ҰĭlqնѺĢԚնк´âÅ¤ӔԚնƢϵԒУͥɿ~ȉԚҎtqQo~Ь̈ն yzαі_ţ̀{b{Ϡǥ̓_ 3 ԄĘǼնՕҀ|αі_ʜūh~_ 6 ԄĘǼǾǏjqQqն yz~Ѻ̓xѺĢԚyƢϵԒУͥɿĺ|եZϓԫɿ_ҠqQj^jն¨â¼àåÞy͈ӡjնѺĢԚǧmնԒУͥɿ_͸ȠjwZ~_ 1 ԄĘǼǾǏj
qQqն¥Þ¨ÓÜå± B ԄĘǼ(glaB)Ϡǥ̓|]Zw¥Þ¨ÓÜå±ͥɿ_ՕҀ|͸Ƞjն<-ÓÜå±նԓɿËàºå±ͥɿ_ǣǫlfy_˘^y{tqQ
дZw 15Dx 20 ˒Ԫ~ȟĎөұգҎZնô~Γԓ͸Ԛ~ͻȉն]ҚʒԐ~çѪʒŤմ˒˷ԐɗäÓÂԓɗäԓɗä´ÂåÞԚյն՟͊ʒŤնÓÂԓЪʒնˮ̴ԓЪʒ~Ť̅ҎtqQДլx yzαі_Ҡ{^tq 3 ԄĘǼ~[sն2 ԄĘǼ|uZwņ^|ψԒ_ҠqQqնfyũ|ψԒ|ӹ_Ҡq~_ 3 ԄĘǼն´ÂåÞԚ_ 1%Ēëǣŷl~_ 3 ԄĘǼǾǏj
qQfԳbo~ď~ԄĘǼx՟͊ʒŤնԓɗäÓÂԓɗն˒˷Ԑɗ|ԫjwնՕҀ{ԀZҥȖx`{^tqQΫǏնԖӵë~Τɿҧ̅jqЬ̈նѨƯͰZЬ̈_ɰq~|uZwh|ϟϹӷwZQ
Functional characterization of RKP (Rice koji protein) disruptants in sake brewing procedure
Teruaki Hanada1.2, Shinichiro Fukuhara1.2, Junko Shitamoto.2, Minori Kono.2, Kazuhiro Iwashita1.2, Osamu Yamada.2(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB )P-18
ÌÔƣʒԒУ porphobilinogen deaminase  Aspergillus nidulans  nitrosative stress ^ĳӁlƮ žˁնթϪș˝, Ҏ˴ɡԜ, ë˼˩č, ˚ԙӏҎ, եӂϒ̲մЈ͞ǫԲäαƱΰǢյɴαΣ~ͥɿƂϽУΣӔ(RNS)|ȘlѐɿƂyo~̴̫ƅȀäӧȀŤԙxեZԫɸʧqwZQ
ЦѺyԒ͆~ RNS ѐɿƂ̴̫|ԫlϟϹώ|ӷwZ_P Ç~o|ԫlϟϹ̨w
Ƞ{ZQ˷ϟϹ Ç~ˍҡ{ RNS ѐɿƂԄĘǼψҠjPo~ѐɿƂ̴̫ҧ˘lfyϑʨjwZ
QofxPA. nidulans ~ԄĘǼÜÊÜÝå~Ȟő~ʘ͜|twPRNS ѐɿǣɤlԄĘǼ AN0121_ƋՂhqQAN0121 ÌÔαƣʒԒУ porphobilinogen deaminase (PBG-D)¨å½jwZԄĘǼxYQ
ЦјȥǏɿҺqЬ̈PPBG-D ЦјӔ|ǾǏjwZqQPBG-D եψΫ̓մPD1յ~αіԙἀմWTյy͈ӡjwեZ RNS ѐɿϥjPPBG-D ψΫʜū̓մALCյ~αі WT ĞZ RNS ѐɿϥjqQqPAN0121 ~ψΫ RNS |twҵȞhqQѺĢŚ~ÌÔ~ƣʒԚ PBG-D ~ψΫԚ|ĬǾlfy˘^y{tqQ Ç|]ZwPFlavohemoglobinմFHbյy nitrite reductase (Nir) RNS ѐɿƂ|ԫÌÔԒУxYfy_ϛwZQWT, PD1, ALC ̓~ FHb y Nir ~ͥɿ͈ӡjqyfP
FHb y Nir ~ͥɿ~ɤh PD1>WT>ALC xYtqQĒë~fy^PPBG-D  RNS |ɼЉjwÌÔ~αƣʒıӷjPÌÔƪˮ RNS ҧ͇ԒУxY FHb y Nir ͥɿƂlfy|twPαі RNS Ƃhnyю\qQ
Heme-biosynthetic porphobilinogen deaminase protects Aspergillus nidulans from nitrosative stress.
Shengmin Zhou, Toshiaki Narukami, Misuzu Nameki, Yosuke Kamimura, Takayuki Hoshino , Naoki Takaya(Graduate School of Life and Environmental Sciences, Univ. of Tsukuba)

47

P-19
̶ǰǧξĢγZqլѺ AoSO ´âÅ¤Ӕ~®¼ß®ɼЉɿšՀ̴̫~ҧ̅Ġę ǐ, Cristopher Sarazar ESCAÑO, õȬ Ήç, ƃ˷ žfմ̂ǫԲäӧαϯäɼαȾյ
VϑϋWSO/Pro40 ϖ̺ǼǃѺз|Τξϋ|ǾǏl´âÅ¤ӔxYPˮɿάѺМҌƣ|ԫîl
fy_ǝƭhwZQլѺ Aspergillus oryzae fyϓƥɿˮl´âÅ¤Ӕ AoSO sPʓR
AoSO _®¼ß®|ɼЉjwԹǤǽ|šՀlfyŧw˘^|jq 1)Qf~fy^PAoSO _ԹǤǽčjqЦјԪӶЮ®¼ß®ĬǾϋ|ūɱjwZƞњɿ_ϥƷhqQj^jPAoSO _®¼ß®ɼЉɿ~šՀϥl̴̫|uZwí˘xYQofxČǅPAoSO ´âÅ¤Ӕ~ƢՑǖ̶ǰhnqǧξĢĥҚjP®¼ß®ɼЉɿ~šՀ]ԹǤǽ~ȥǏ|ɹҞ{Ցǖ~ƥȉұqQVˎ͜]Ь̈WAoSO | EGFP Ҍƣjq´âÅ¤ӔψΫhnPЦјŚ~ AoSO ~ʩŻҋŏՕɴԦ|twҥȖjqQ1195 ÓÂԓ^ʒȿǫ{ AoSO ´âÅ¤ӔŹΪѫbҧ̅lqPmȊգʻ
ĥ~Вį{ţѰԒ͆ Saccharomyces cerevisiae γZPˆǪb~̶ǰǧξĢψΫä®¤Ýå¿â¥lˎ͜ʮγjqQS. cerevisiae ~ЦјŚ|]Zw AoSO-EGFP _®¼ß®|ɼЉjwΔΥ~šՀ̫ӵɦʒlfyʨ̮|PAoSO ~ 556-1146 ÓÂԓ~Ցǖ_®¼ß®ɼЉɿ~šՀ|ɹҞ^uƆŤxYfy_ϢҴhqQ̷|PA. oryzae |]Zwf~̶ǰǧξĢ AoSO[556-1146] EGFP yҌƣjwψΫhnqЬ̈PЦјӔ|®¼ß®ɼЉɿ~šՀ_ҥȖhq_PԹǤǽ|Ҡ{^tqQΫǏPAoSO _ԹǤǽΤξϋ|šՀlq|ɹҞ{Ցǖ~ƥȉұwZQ
1) J. Maruyama et al. (2010) Biochem. Biophys. Res. Commun. Vol. 391, 868-873
Deletion analysis of A. oryzae AoSO protein for its aggregation mechanism in response to stresses
Kei SAEKI, Cristopher Sarazar ESCAÑO, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo)

P-20
Aspergillus nidulans |]d ypdA ԄĘǼψΫūɱ̓~ҧ̅лȺҘѫ նѽƐǫϨ նƤҠƻ նԯԌ˅ʇ  մ ̂ƃǫԲäαΣβ̧Ŵʒ ̂ƃǫä˵̀ϟΫƇѿǫäϖѺƅյ
His-Asp ÝâԓÝßåʉǝĘӿНÄ¤ºÝ^եЇ̤Σ|xĳǾhqˢӼϋ{ΰǢɼЉ̴̫xY
МΥѺxɑbĳǾhwZQʓRնfx| Aspergillus nidulans ~եͫӱǎɼЉЫӚ|uZwҧ̅jÆ®¶­â¢¾å± NikA ÝâԓӞϱôԪǆǼ YpdA ß®Ñâ®ß£Øßå´åSskA ] SrrA _ͫӱǎɼЉնԓƂ®¼ß®ɼЉ{z|ԫîjwZfy˘^|jw`qQqԄĘȀϋ|̶ʷ_ѧ̾xYfy_ү˘hwZ YpdA |uZw alcA ËàÖå´å| ypdA ԄĘǼ~ψΫūɱƞњ{Conditional-ypdA ̓մCypdA ̓յӵʒjնYpdA ~̴њ|uZwҧ̅ӷw`qQj^j{_նYpdA
̶ʷ_ѧ̾y{Ɛǆ~Τȉ|ѦtwZ{ZQfx~ҧ̅xնFGSC A89 ̓Ҥ̓yjq CypdA ̓Ĩγjw`qQ˷̓Ūγx`̑՜Ҟ͍ɿÒå å_újbնq ypdA ~ψΫūɱ_íƆŤxYfyçǆy{նh{̴њҧ̅_ǈՃ|{uuYtqQofxČǅնYpdA ~̴њ|uZwh|έҧͰqնćԎ|ǪԘǧξ~ĥţ_ƞњxYնқˆ~ԃʡÒå åŪγx` ABPU1 ̓Ҥ̓yjq CypdA ̓~ӵʒұqQqնalcA ËàÖå´å~ӶЬԎŦҠϒlfy|ūɱΥʏ_ѫǴ{ CypdA ̓ƚɰjq~xնo~ғΫǔ~ҧ̅Ь̈|uZwǝƭlQ˷̓ ypdA ψΫʜū˿Ĕ|]
Zw~ՕҀ{αіԮȍϥjնypdA ψΫʜūìx~ HOG ЫӚ~ͥɿƂҴqQΫǏն˷̓Ҥ̓
yjw sskA ] srrA ԄĘǼ~Ϡǥұyy|նͫӱǎ®¼ß®]ԓƂ®¼ß®|ȘlɼЉ|uZwҧ̅jw]նYpdA ̶ʷ_ѧ̾y{Õ ¿¯Ô|uZwюȖjwZQմ˷ϟϹαϟ°â´åʽʶƛdqQյ
Analysis of the conditional-ypdA strain in Aspergillus nidulans
Yura Midorikawa1, Daisuke Hagiwara2,3, Akira Yoshimi 2, Keietsu Abe1,2(1 Grad. Sch Agric. Sci., Tohoku Univ., 2 Tohoku Univ. NICHe , 3 Present address: MMRC, Univ. of Chiba)

48

P-21
МΥѺ Aspergillus nidulans~Æ®¶­â¢¾å±PhkA,PhkB~̴њҧ̅ĠƝϛшȬȶ^ԛõĉǼŷ҇ԿǦ ȟ̇ƶǮմƦǫԲäαƱӧƦǕǫäӧ յ
VϑϋWМΥѺ A. nidulans | 15ϵ~Æ®¶­â¢¾å±մHKյ ϵ~ ÝâԓӞϱôԪǆǼ HPt ,4ϵ~ß®Ñâ®ß£Øßå´åմRRյ_ǾǏlQ15ϵ~HKԄĘǼ~[sphkA , phkBop
Schizosaccharomyces pombe ~ phk1/mak2 , phk2/mak3ƕphk3/mak1 yեZϓƥɿϥlQf~ 3 u~
HK  S. pombe |]ZwˮɿάԓƂ®¼ß®ɼЉˮМŤҕ~ūɱ|ԫtwZfy_lx|ǝƭh
wZպյQ˷ϟϹxA. nidulans |]d PhkA,PhkB ~αέϋɫų˘^|lqphkA ƕ phkB ԄĘǼϠǥ̓yҵȞψΫ̓ĥҚj®¼ß®|ȘlɼЉαі~ǧƂҥȖjqQVˎ͜yЬ̈W2 ˒ԪǗ՜~ŤαǼӽԓƂ͋УƪǗǑ|®Ñ¸¼jαіҥȖjqyfphkA
Ϡǥ̓ӽԓƂ͋У 1.5mM xphkB Ϡǥ̓ 2mM |]Zwԙἀy͈ӡjwԓƂ®¼ß®~ʎƛɿ_եb{tqQqԓУūԱ˿Ĕ|]ZwphkB ҵȞψΫ̓ѺМ~ěԧ|ǧƂ_ҠqQΫǏÝÞ´Ô PCR |1)

´Üå±ԄĘǼ NADPH ¢¬µå±ԄĘǼ~ψΫ|uZwҧ̅ӷwZQNakamichi,N.et al. (2002) Biosci.Biotechnol.Biochem.66(12):pp.2663-2672

Characterization of Histidine kinase PhkA,PhkB in Aspergillus nidulans
Tomomi Sako,Yukari Yamazaki,Kyoko Kanamaru,Masashi Kato1,Tetsuo Kobayashi(Grad. Sch. of Bioagricultual Sciences, Nagoya Univ. 1Sch .of Agriculture , Meijo Univ)

P-22 (O-1)
լѺ A. oryzae ~ MAP ¢¾å± AoFus3 yϓăĥγlˍҡ´âÅ¤Ӕ~̴њҧ̅ϚѽǫӏնõȬΉçնÖzgür Bayram1նOliver Valerius1նGerhard H. Braus1նƃ˷žfմ̂ǫԲäӧαϯäɼαȾն1 §¸ºâ§âǫȀյ
VϑϋWϖ̔αΣ|ɑbĳǾhwZ MAP ¢¾å±նǩԌ^~ʉǝĘӿlfyxЦјͥŻūɱjwZQԒ͆ Saccharomyces cerevisiae |]ZwնMAP ¢¾å±~yu Fus3 ˮɿά|]ZwնʰƣÉàÖâɼЉyЦјҌƣ|ԫîlQМΥѺ~ Fus3p ÏÖà¥ˮɿά|ԫîlfy|ŷ\նĂ̷đҾնςƐɿЇ|]ZwԘҞ{Ň`ˮlQʓRĒŮնA. oryzae ~ Fus3p ÏÖà¥xY AoFus3 _ѺМŎЀyԹǤǽ|ȥǏjնo~ԄĘǼϠǥ̓ѺМʒԧ~ξɉyŤαǼɦʒ~ĞìϥlfyҠţjqQqնAofus3 Ϡǥ̓xԽʰlЦј|ͿѺ_Ęʺlųƣ_ë˖jqfy^նAoFus3 _ԹǤǽčjqЦјԪӶЮ|ԫİlfy_ϥƷhqQf~[{МΥѺ~ Fus3p ÏÖà¥~Ǫ̬{̴њ|]dŤǼ
̴̫|uZwPyz˘^|{twZ{ZQČǅPf~̴̫ҧ˘lqնA. oryzae |]Zw

AoFus3 yϓăĥγl´âÅ¤Ӕ~ƥȉy̴њҧ̅ҎtqQVˎ͜äЬ̈WAoFus3 ~ C ˶Ѐ| TAP(Tandem Affinity Purification)´¥ӶЬjqҌƣ´âÅ¤Ӕն
Aofus3 Ϡǥ̓|ψΫhnqQ´¥γZqЙҚ] LC/MS/MS ҧ̅| AoFus3 y~ϓăĥγϥl´âÅ¤Ӕƥȉjնo~{^x̴њ˵ϛ{ 2 ϵ~´âÅ¤Ӕ|uZwҧ̅ҎtqQRACE ҧ̅|ԄĘ
Ǽ~ĀͻЏǉ_̺jZfyϢҴjq~sϠǥ̓ĥҚjնғΫǔҧ̅ҎtqЬ̈նòяy|ŤαǼɦʒ~Ğìϥjն^uƢR~̓xξ{tqѺМɦʏ~ǧƂϥjqQƥ˞| EGFP Ҍƣ´âÅ¤ӔψΫhnwȥǏҧ̅jqyfնԹǤǽyЦјӔ|½¸¼Υ|ȥǏjwZfy_ҥȖhqQΫǏնëҬ 2 ϵ~´âÅ¤Ӕy AoFus3 y~̴њϋԫİ̥ҫjwZQ
Identification and functional analysis of novel AoFus3(MAP kinase)-interacting proteins in Aspergillus oryzae
Daiki YAHAGI, Jun-ichi MARUYAMA, Özgür BAYRAM1, Oliver VALERIUS1, Gerhard H. BRAUS1, Katsuhiko
KITAMOTO (Dept. of Biotechnol., Univ. of Tokyo,1Georg-August-Universität Göttingen)

49

P-23
ςƐϖѺ Aspergillus fumigatus |]dͫӱǎɼЉЫӚ~̴њҧ̅ѽƐǫϨնĄÂąӱնȺ˷ӷմƇѿǫäϖѺ°â´åյͰǏɿϖѺσPöyjwŐπŵ~ĞìjqȐö|ʎ̍lqѧ̾Ϊ_եZQΫǏPŪγƞњ{ʞϖѺ҆ս¤Ü®|ԱPˍǱ{͘υ҆_ɤb˲wZQ˷ϟϹx®ÎÞ£Þ®σ~ö{ƐǆѺxY Aspergillus fumigatus |]ZwPˍǱʞϖѺŰ~̮ϋy{[ͫӱǎɼЉʉǝĘӿН~ŤǼԄĘȀϋҧ̅ҎZPo~ƞњɿҰĭlQ
Ǫb~Ԓ͆МΥѺ|]ZwPͫӱǎɼЉЫӚĂʒŤūɱНy HogA/SakA MAPK ®¦å½|tw̫ʒhPͫӱǎ®¼ß®Ēǩ|Ǫ̬{ΰǢ®¼ß®|ȘlɼЉơtwZQΤ|PHAMP ½ÕâÝÈå¼ N ˶Ѐľ|ʧuÆ®¶·â¢¾å± NikA PÉÞ­¢²¿Þ{z~ӧ҆ƂƣΣ~αіԮȍŹ̈|ͰbԫîjP҆ŰĥγΔxYyʱͻhwZQqPHogA/SakA MAPK եͫӱǎ®¼ß®]ÉÞ­¢²¿ÞŢέ|twÝâԓƂմͥɿƂյjPo~ìͦǆǼ_ψΫɼЉlQČǅPA. fumigatus |]dͫӱǎɼЉЫӚ~̴њ˘^|lq|PNikA ] SakA MAPK ~ԄĘǼϠǥ̓ĥҚjqQNikA ԄĘǼϠǥ̓ÉÞ­¢²¿Þ|ѐɿϥjPեͫӱǎ®¼ß®|Șjw
ɤZʎƛɿϥjqQçˎ SakA ԄĘǼϠǥ̓PԙἀyƥЇ~ÉÞ­¢²¿ÞʎƛɿϥjPNikA
ԄĘǼϠǥ̓ɢZ_եͫӱǎ®¼ß®ʎƛɿϥjqQqP ´Üå±ԄĘǼ catA ~ψΫ_ SakA ĬǾϋ|եͫӱǎ®¼ß®]ÉÞ­¢²¿Þ|twë˖jqQf~ɿӔ A. nidulans |]dҧ̅Ь̈yϓĜjwZ_PSakA ԄĘǼϠǥ̓~ŤαǼ_®¼ß®ʎƛɿϥh{^tqΔξ{tqQ
Functional analysis of HOG pathway in opportunistic pathogen Aspergillus fumigatus
Daisuke Hagiwara, Tohru Gonoi, Susumu Kawamoto(MMRC, Chiba Univ.)

P-24
Neurospora crassa ~ MAK-1 ƕ MAK-2 MAP ¢¾å±~Ϡǥ̓~ғΫǔyȥǏҧ̅þąҶù, ե̳̺Ʋ, çϝ˝ɧ, ҇˼ϖմ̂͢ǫԲäαƱϯȀյţѰԒ͆x, ЦјǤ~ʷń|ɼЉjw Slt2p MAP ¢¾å±|twЦјǤ̫А (cell wall integrity:CWI)_ƑȒ|ūɱhwZQ˜ɍɗ, Neurospora crassa ~ MAK-1 (ţѰԒ͆ ЦјǤ̫АЫӚ Slt2p Þ²à¥), ΖɿјǼψѰ^ѺМαіӽϳ|]Zwʁɉϋ|ÝâԓƂjwZfy, MAK-2 (ţѰԒ͆~ PheromoneɼЉЫӚ Fus3p Þ²à¥) _ beta-1,3-glucan ƣʒԒУԮȍŰ micafungin |ЦјǤʷńŢέ|twՕ
Ҁ|ÝâԓƂhfyǝƭjqQN. crassa ~ 2 ϵՖ~ MAP ¢¾å±~ CWI ~ӉΧɗ˘^|lq, Δmak-1 ̓yΔmak-2 ̓~ϵR~ЦјǤʷńŰ|ȘlʎƛɿҺqQòϠǥ̓ Calcofluor White Congo Red |ȘjwԙἀyƥЇ~ʎƛɿxYtq_, micafungin y SDS |Șjwŕ|ՕҀ{ʎƛɿϥjqQ¢¶âαƣʒԮȍŰ polyoxin  nikkomycin |ȘjwΔmak-1 ̓~_ԙἀեZʎƛɿxYtqQ̷|, MAK-1 y MAK-2 ~ȥǏҺq, Ƣ MAP ¢¾å±y GFP y~Ҍƣ´âÅ¤Ӕβαl̓ĥҚj, ҋŏՕɴԦxҥȖjqQmak-1-sgfp ̓~ѺМ|]Zw, MAK-1 ЦјǤʷń®¼ß®
~ˮΖ|ԫİ{b̔|ȥǏjwZqQf, MAK-1 ~ʁɉϋÝâԓƂy~ԫӶ_ϥƷhQq, mak2-sgfp ̓xѺМαіӽϳ|]d MAK-2 ~ȥǏҴ{^tqQΔmak-1 ̓Δmak-2 ̓, ΖɿјǼ~ɦʏξɉψѰ~ӹɝ, ψѰЌԪƥǦ~Ҍƣњ~̶ʷ_Ҵfy^, ΫǏ, ΖɿјǼ|]d
MAK-1 ƕ MAK-2 ~ȥǏɿ|uZwҧ̅ӷwZQ
Localization of two MAP kinases, MAK-1 and MAK-2 in Neurospora crassa.
Masayuki Kamei, Masakazu Takahashi, Akihiko Ichiishi and Makoto Fujimura(Grad. Sch. of Life Sci. , Toyo Univ.)50

P-25 (O-3)
Aspergillus nidulans |]dեͺ˿Ĕìx~Ëàºâ¢¾å± C |Ñ¼å¬®ҵȞʜū̴̫~ҧ̅ΡȬήýնǙŚҘù, ǭδ˘ɵ մ̂ǫԲäӧαϯäɼαȾյ Ëàºâ¢¾å± CմPKCյϖ̔αΣ|եɗ|ĳǾhq°Ýâո®ß¿â¢¾å±xYQ
Aspergillus nidulans ~ PKC ¨å½l pkcA ԄĘǼαі|ɹՐ{ԄĘǼxYfy_ϥhwZ 1)QɥϟϹȋ|]ZwĥҚhq pkcA ͺɗʎƛɿ̓ 30Dxԙαǔ̓yƥ̬~ғΫǔϥl_, 42Dxαіlfy_x`{ZQ42D|]Zw pkcA ͺɗʎƛɿ̓~ŤαǼx, ӳɉ~ŤαǼψѰ˞|ҠΖ
̨ɿαԧ_Ӳôxļ̹j, ψѰЌ~ɦʒ_Ҡ{ZQh|Pf~y` DNA ~қҚӗf_, o~ɮlc DNA _Ťҧhq 2)QpkcA ͺɗʎƛɿ̓~ŤαǼ 42DxǗ՜jqy`, Ñ¼å¬®ҵȞjqЦј|Τξϋ|ҠͥɿԓУմROSյ~҂ϸ, DNA ~ˌΡƂ_Ҡqfy^, եͺ˿Ĕì|]ZwψѰ˞| PkcA _ǰͥlyÑ¼å¬®_ҵȞhfy_ϥƷhqQPkcA |twūɱhfy_ϥƷhwZ MAP ¢¾å± ®¦å½~Ñ¼å¬®ūɱ~ԫî|uZw̥ҫlq, f~ MAP ¢¾
å± ®¦å½~̫ʒǆǼ¨å½l bckA, mpkA op~̶ǰ̓~ҧ̅ҎtqQf~̶ǰ̓ӳɉ~ǗǑ|]Zw 42Dxαіlfy_x`{^tqQh|, f~̓~ŤαǼ 42DxǗ՜jqyf, ROS ~҂ϸ, DNA ~ˌΡƂ_ҠqQf~fy^, եͺ˿Ĕì|]Zw PkcA Ñ¼å¬®~ʜū|ɹҞxY, f~̴њ BckA, MpkA ƪ MAP ¢¾å± ®¦å½|ĬǾlfy_ϥƷhqQ1) Ichinomiya, M., et al., (2007) Biosci. Biotechnol. Biochem., 71, 2787-27992) ΡȬ, ˒˷ӧѯƂȀė 2010 ɍɗǫėҽ΄Ҟ˓Հ, p213
Analysis of PkcA function in the suppression of apoptosis under heat stress condition in Aspergillus nidulans
Takuya Katayama, Hiroyuki Horiuchi and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)

P-26
լѺ Aspergillus oryzae |]dɴȟЌɦʒôɸԫӶ´âÅ¤Ӕ AoApsB ~̴њҧ̅ȺκЯɌPϚѽǫӏPõȬΉçPƃ˷žfմ̂ǫԲäӧαϯäɼαȾյVϑϋWŻΣ~£×¸ËЬƣ̤Σ~ƐɦӔӶЮyƥ̬|նМΥѺԹǤǽčjwЦјԪӶЮҎ[նǪЦјαΣyjwŕӳjq¬®ºÔˮjwZQAspergillus nidulans |]ZwɴȟЌɦʒôɸ(microtubuleorganizing center)ԫӶ´âÅ¤Ӕ ApsB _ԹǤǽ|ȥǏly~ǝƭ_YնЦјԪӶЮ|ԫîjwZƞњɿ_ϥƷhwZQʓRĒŮնA. oryzae |]Zw MAP ¢¾å± AoFus3 _ԹǤǽ|ȥǏlāψҠ
jq_նo~ȥǏÅ´åâ ApsB yƥ̬|ԹǤǽ~ôɸǉ[Ýâ¥ΥxYtqQ˷ϟϹxնMAP ¢¾å±yɴȟЌɦʒôɸԫӶ´âÅ¤Ӕ_ԹǤǽ|]ZwƊƥjwЦјԪӶЮūɱjwZyZ[ēҷ̥үlqնA. oryzae |]dɴȟЌɦʒôɸԫӶ´âÅ¤Ӕ~̴њҧ̅ҎtqQVˎ͜]Ь̈WA. nidulans apsB ԄĘǼ~ԎŦγZw A. oryzae ~§ÂÔ»å´Íå®x BLAST ̥ФjqЬ̈նϓƥɿϥlԄĘǼ AO090020000040 ҠţjնAoapsB yƱƦjqQAoApsB ~̴њҺqն
AoapsB Ϡǥ̓ĥҚjqQȔǬǗǑë~¨à¿å|͋ŷ\wŎЀЦј~ͿѺҵȞlĞͫӱǎ¬Ú¸¤Ȋգ~Ь̈նAoapsB Ϡǥ̓xԽʰlЦј|ͿѺ~Ęʺlųƣ_ǣŷjնAofus3 Ϡǥ̓yƥ̬~Ь̈_ɰqQɴȟЌɦʒôɸԫӶ´âÅ¤Ӕ_ͿѺ~Ęʺԭc̴њufyϥjq~ն˷ϟϹ_ŧwxYQΫǏնAoApsB y AoFus3 op~ԹǤǽȥǏ|ԫl̴њϋ{ϓăĥγ|uZwҧ̅Ҏ
twZQ
Functional characterization of microtubule-organizing center component AoApsB in Aspergillus oryzae
Junpei KAWABATA, Daiki YAHAGI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo)

51

P-27
լѺ A. oryzae ~ʧuЦјӔǔÏ®ÏÝÅå± A2 ̬´âÅ¤Ӕ AoPlaA ~̴њҧ̅բąОùնȟӂ˗Ɍնƃ˷žfնˮȮ Ȁ մ̂ǫԲäӧαϯäɼαȾյVϑϋWÏ®ÏÝÅå± A2 (PLA2) ¥Ý°àÝâћӔŷ͋ŤҧjնћѕԓyÝ³ÝâћӔӺՂlԒ
УxYQf~[sЦјӔǔ PLA2 (cytosolic PLA2; cPLA2) ŻΣ|]ZwƛȏĢŬ΍|ɼkwȟјĢ©Þ­Ģѡ^Ü¢½âԓӺՂjն¨ªÂ½Ֆ{zћӔÕ»å´å~βα|ԫîlfy_ϛwZQçˎնʓRМΥѺ|]Zw cPLA2 ̬~ԎŦ_ǾǏlfyҠţjնլѺ~ʧuƸç~cPLA2 ̬´âÅ¤Ӕ AoPlaA _Ó¼¨â½ÝǩѡyŚѡ~Ԫ~ѡԪ®Îå®|ȥǏlfy{z˘^|jq_նo~αέ̴њí˘xYQ˷ϟϹx AoPlaA ~ҳЦ{̴њҧ̅ϑϋyjqQVˎ͜äЬ̈WAoPlaA o~ȥǏ^Ó¼¨â½Ý~ћӔđҾ|ԫîlƞњɿ_ю\qQofx
AoplaA եψΫ̓äϠǥ̓y¨â¼àåÞ̓|uZwЗÓ¼¨â½ÝθŤҺҚjնo~ÝâћӔЪʒ
MS/MS ҧ̅|͈ӡjqQo~Ь̈նAoplaA եψΫ̓x 36:3  36:4 yZ[ԥԧ~ћѕԓʧuÏ®
É¶­Þ´ÂåÞÓâ (PE) _͸Ƞjw]ն34:1  34:2 ~ћѕԓʧu PE _ǣŷjwZqQçˎն
AoplaA Ϡǥ̓|]Zw Þ­ÝÈâԚ~ǣŷ_ҴqQдZw̬R{Ǘ՜˿Ĕ|]ZwƢ̓~αі͈ӡҎtqyfն¥Þ¨å®ΓУͽylˬȠǗǑ|]Zw AoplaA եψΫ̓~αі_ĞìlfyҠţjqQqնf~αіĞì 15°C xǗ՜jq PD ǗǑ|]ZwҴqQΫǏնĀʊͥɿôɸ̀ǘ|ǧξȞőjq AoPlaA եψΫl̓ĥҚjնեψΫ̓|]dαі~ĞìyÝâћӔЪʒ~
ǧƂ|z~[{ϓԫ_Ҡ^{zնh{ҧ̅ҎtwZQ
Functional analysis of cytosolic phospholipase A2-like protein AoPlaA in A. oryzae
Noriyuki Komai, Shohei Kotani, Katsuhiko Kitamoto, Manabu Arioka(Dept. of Biotechnol., Univ. of Tokyo)

P-28
լѺ A. oryzae |]d Ca2+ՊĬǾɿÏ®ÏÝÅå± A2 ̬´âÅ¤Ӕ iPlaA ~̴њҧ̅ȟ̳Ɯѓնƃ˷žfնˮȮ Ȁ մ̂ǫԲäӧαƂäɼαȾյÏ®ÏÝÅå± A2 (PLA2) ÝâћӔ~ʧu¥Ý°àåÞդ̖~ sn-2 ĝ~®ºÞЬƣŷ͋ŤҧjնћѕԓyÝ³ÝâћӔαʒlԒУxYQPLA2 öyjwŤ͛ǔ~ secretory PLA2 (sPLA2)նЦјӔȥǏǔ~ cytosolic PLA2 (cPLA2)ն] Ca2+ՊĬǾǔ~ Ca2+-independent PLA2 (iPLA2) ~ 3 u~´Ë|Ťd_նf~[s iPLA2 ŻΣ|]ZwΒσƘɼÑ¼å¬®{z̬R{αέ̴њ|ԫîlfy_Ť^twZQʓRfxլѺ A. oryzae ~ʧu 2 u~ sPLA2 (sPlaAնsPlaB)  cPLA2 ̬´âÅ¤Ӕ(AoPlaA) ~ҧ̅Ҏtw`q_նČǅÆ¼ iPLA2 yϓƥɿϥl A. oryzae iPlaA (AO090011000913) Ҡţjնo~ҧ̅ҎtqQ
DPY ͭĢǗǑxαіhnq A. oryzae ~ cDNA y|նDOGAN ë~Āͻ_̺jZ^ RACE ҧ̅]
 PCR ҧ̅|tw̥ҫjqQo~Ь̈նiplaA ԄĘǼo~ӞŞԩǷΔ_Āʊ ORF ~П 200 bp zëͦն
ЩЬΔ_ 300 bp zìͦxYնDOGAN ë~Āͻyçѧjնâ¼àâ 2 uʧuŒԧ 601 ÓÂԓ̀ǘ~´âÅ¤Ӕ¨å½l~yʱȉhqQqնiPlaA-EGFP ψΫl AIE ̓ĥҚjw®´âÊà¸¼ҧ̅ҎtqyfնĀʊª¯xYП 90 kDa ~ĝц| EGFP Ҍƣ´âÅ¤Ӕ~Äâ½_ҴqQ̷|նAIE ̓ҋŏՕɴԦxҥȖj iPlaA ~ȥǏҺqyfնͭјԳbЦјӔŒĢ|ŤɄjwZfy_ϢҴhqQqնԹǤ~ȥǏҥȖhqQΫǏնiPlaA ~̴њ˘^|lqնԄĘǼϠǥ̓~ĥҚҎtwZQ
Identification and characterization of putative Ca2+-independent phospholipase A2-like protein iPlaA in A. oryzae
Sou Kohashiguchi, Katsuhiko Kitamoto, Manabu Arioka(Dept. of Biotechnol., Univ. of Tokyo)

52

P-29
լѺ A. oryzae |]dâ½ª¼å¬®ԫӶԄĘǼ aipC, aipD ~ҧ̅̃ȣӓĊն̭ƜҘ̷ԋնˮȮ Ȁնƃ˷žf մ̂ǫԲäӧαϯäɼαȾյѺМŎЀ|]Zwնâ½ª¼å¬®_ͥψ|ҎwZԌĝ|ȥǏl AoAbp1  bait yjq
yeast two-hybrid ®¤Ýå¿â¥|twն4 u~ԄĘǼ aipAքD (AoAbp1 interacting protein) _Ҡţhq 1)Q
f~[s AipC ţѰԒ͆ S. cerevisiae ~ App1p (actin patch protein) ~ÏÖà¥xYնy|ˑϛ~½Õâʧq{Z_նď~ Aspergillus ȪМΥѺ|եZϓƥɿˮlÏÖà¥_ǾǏjwZqQqնAipD
 AoAbp1 ~ SH3 ½Õâyϓăĥγlyю\ËàÝâÝ¸¶{½Õâˮjw]ն
Aspergillus ȪМΥѺ|]Zw͈ӡϋեZϓƥɿxÏÖà¥_ǾǏlçˎxնS. cerevisiae |ÏÖà¥ǾǏnmնМΥѺ|ǋˮ~´âÅ¤ӔxYyϥƷhqQ˷ϟϹxնf~ 2 ԄĘǼ|uZw̴њҧ̅Ҏ[fy|նA. oryzae |]dâ½ª¼å¬®̴̫~˪{έҧϑʨjqQ
mնaipC ] aipD ~ RACE ҧ̅ҎZնӞŞԩǷΔyЩÿΔնâ¼àâ~ĝц{z͑ȉjqQo~Ь̈նAipC  798 ÓÂԓ̀ǘնAipD  399 ÓÂԓ̀ǘ^op̫ʒhնf~[s AipD â¼àâ 1 ãʖƪfy_˘^|{tqQ̷|նf~ʉǝy|նaipC y aipD ~Œԧ cDNA ~¤àå¿â¥]o~ԄĘǼϠǥ̓ĥҚjqQČɮնòԄĘǼ~Œԧ cDNA ¤àåâγZq
AoAbp1 y~ yeast two-hybrid |ϓăĥγҧ̅]նϠǥ̓γZqғΫǔҧ̅Ҏ[ĀȉxYQ1) ̭ƜնЅ 9 ǅМΥѺŤǼαΣȀ¨âÉßâ®Ҟ˓Հ p. 31
Analysis of aipC and aipD related to endocytosis in Aspergillus oryzae
Kento MATSUO, Yujiro HIGUCHI, Manabu ARIOKA, Katsuhiko KITAMOTO(Dept. of Biotechnol., The Univ. of Tokyo)

P-30
լѺÒÞ¼å®ÅåÓå±~ƢϵΓУͽ|ЦјŚȥǏǧƂɌ˷ ƶýնǫӾƜ ͮǼնδô ίɁնˍӂ ȡɠնĄƯ žý(̂ƃǫԲäӧäαΣβ̧Ŵʒ)ʓRնլѺ~»âËâŤҧԒУ~αβ|]ZwնҵȞǘӔxYÒÞ¼å®~ЦјŚƚөН_ԘҞxYնo~ƚөʤ[ÒÞ¼å®ÅåÓå±(MalP)ԄĘǼ_¥Þ¨å®|ӞŞßÍÞxʜūƛd
wZfylx|˘^|jwZ 1)QçˎնţѰԒ͆xնÒÞ¼å®ÅåÓå±~ψΫ_ӞŞßÍÞxʜūƛdyy|ն´âÅ¤ӔßÍÞxâ½ª¼å¬®ĬǾϋ|ͭјӥӯhŤҧƛd
fy_ǝƭhwZQլѺ|]Zwƥ̬~Ťҧ̴̫~ǾǏ_Āʊh_նo~ˮΖ˵rí˘xYQofxն˷ϟϹxնMalP ~ΰǢ¬¥¾ÞɼЉ|]d´âÅ¤ӔßÍÞx~Ťҧ̴̫ҧ˘lq|ն¥Þ¨å®kylƢϵΓУͽǾǏì|]d MalP ~ЦјŚȥǏ|uZwҧ̅ҎtqQ
GFP-MalP Ҍƣ´âÅ¤ӔլѺxψΫhnնҋŏՕɴԦҥȖҎtqyfնÒÞ¼å®ҵȞ˿ĔìxöyjwЦјѡ| GFP-MalP ~ȥǏ_ҥȖhqQf|¥Þ¨å®ʹŷlyӴ^|Цјѡ^~
GFP ҋŏ~ͬǰyͭј~ϱҎ_ҴqQqնÉÞ¤¼å®ÒâÂå®~ǾǏì|]ZwЦјѡ^~ GFP ҋŏ~ͬǰ_Ҵն¥Þ¨å®¾à¥xY 2-»¢¬¥Þ¨å®ն3-Õ¶Þ-O-¥Þ¨å®ն6-»¢¬¥Þ¨å®~op|Șl MalP ~ЦјŚȥǏɼЉ|ɀ_ҥȖhqQf~fy^ն
MalP Ԓ͆~ÒÞ¼å®ÅåÓå±yƥ̬|â½ª¼å¬®|Ťҧūɱƛd~~նК|ȘlɼЉ|ԀZ_Yƞњɿ_ϥƷhqQ
1) Hasegawa et al. Fungal Genet. Biol. 47, 1-9 (2010).
Intracellular localization of maltose permease (MalP) in response to carbon sources in Aspergillus oryzae
Tetsuya HiramotoնRyoko DaidouguchiնMizuki TanakaնTakahiro ShintaniնKatsuya Gomi
(Div.Biosci.Biotechnol.Future Bioind.,Grad.Sch.Agric.Sci.,Tohoku Univ.)53

P-31 (O-4)МΥѺ Aspergillus nidulans |]d<-1,3-¥Þ agsA, agsB ĂԘϠǥ̓~̴њҧ̅âƣʒԒУԄĘǼ agsB Ϡǥ̓]϶ѿ̞ 1, ƤҠƻ 2, ԯԌ˅ʇ 1,2մ1 ̂ƃǫԲӧäαΣβ̧Ŵʒ, 2 ̂ƃǫä˵̀ϟյ
ʓR, Aspergillus nidulans ~ЦјǤ̫А¬¥¾ÞĘӿЫӚ|uZw, MAP ¢¾å± MpkA ЫӚôɸ|ҧ̅ӷw`qQfx|, ˷Ѻ~ MpkA ЫӚ, ţѰԒ͆|ǾǏj{Z<-1,3-¥Þ âƣʒԒУ
մAGSյԄĘǼ agsA, agsB ~ӞŞūɱ|ΤƂjwZfy˘^|jwZQq, ˷Ѻ|]d 2 ϵ~ AGS ԄĘǼ~̴њ˘^|lq, agsA Ϡǥ̓, agsB ψΫʜū̓γZw AGS ԄĘǼ~̴њҧ̅ӷw`qQo~Ь̈, agsA ԄĘǼӳɉαі˿Ĕìx yz̴њjwZ{Zfy, ˷Ѻ|]d<1,3-¥Þ â~ƣʒ|ö| AgsB _ԫîjwZfy_ϥƷhw`qQj^j{_, agsB ԄĘǼ~ѧ̾ɿ] AGS ԄĘǼȅŒ̶ʷ̓ƚɰ~ƞƩ|uZw˘^|hwf{^tqQČǅ, fxƚɰ_x`wZ{^tq agsB Ϡǥ̓~ӵʒ|ʒŶj, agsB Ϡǥ̓~ғΫǔҧ̅Ҏtq~xo~Ь̈|uZw
ǝƭlQ agsB Ϡǥ̓, Congo Red |Șlʎƛɿ~ë˖{z agsB ψΫʜū̓xҴqΤɶϋ{ғΫǔϥjqQq, ЦјǤʒŤʣţj, NMR ҧ̅Ҏtqyf, <-1,3-¥Þ â~¬¥¾Þ̥ţ
h{^tqQf~fy^, agsB ԄĘǼ<-1,3-¥Þ â~ƣʒ|ɹՐxYfy_ɤbϥƷhqQh|, agsA Ϡǥ̓Ҥ̓yjq agsB Ϡǥ̓~ӵʒұ, Aspergillus ȪѺ|]Zwŧw AGS ԄĘǼȅŒ̶ʷ̓ƚɰjq~x, f~̓~ғΫǔҧ̅~Ь̈|uZwюȖjqQմ˷ϟϹαϟ°â´åʽʶƛdqQյ
Analysis of the disruptant of <-1,3-glucan synthase gene agsB and the double disruptant of agsA and agsB in
Aspergillus nidulans
Azusa Inaba 1, Akira Yoshimi 2, Keietsu Abe 1,2( 1 Grad. Sch. Agric. Sci., 2 Tohoku, Univ., NICHe.,Tohoku Univ.)

P-32
Aspergillus nidulans ~¢¶âƣʒԒУ CsmA ~ѺМŚȥǏƂ|]d¢Á¬â~ɫųȝȶϖ̦PǙŚҘùPǭδ˘ɵմ̂ǫԲäӧαϯäɼαȾյ ¢¶âМΥѺЦјǤ~öҞ̫ʒʒŤ~çuxYPo~αƣʒɦʏɦʒPŤƂ|ԘҞ{ɫųʧuQ
A. nidulans |P¤Ü® V |Ȫl¢¶âƣʒԒУ¨å½lԄĘǼ csmA _ǾǏjPf~¤Ü®~¢¶âƣʒԒУԄĘǼѺМǔ~αіϥlϖѺՖ|~ǾǏlQCsmA ѺМ~αԧ|ɹՐ~̴њʧsP
N ˶Ѐľ|Ó¬âyϓƥɿ~Y½ÕâˮlQfx|ɥϟϹ¥ÞåËxPCsmA _¢Á¬â
KinA y~ϓăĥγ|ѺМŎЀĐӪӥӯhȥǏƂlfy˘^|jq 1)QČǅPCsmA ~ѺМŚȥǏƂ̴̫|uZwPKinA yũ~¤Ü®~¢Á¬âxYPѺМŎЀαԧ|̴њlfy_ϥƷhw
Z KipA ~ɫų̥ҫjqQkipA _ϠǥhqԄĘϋїˣ|]ZwPԙαǔ CsmA ~đ| EGFPCsmA ψΫl̓ĥҚjPEGFP-CsmA ~ȥǏҥȖjqЬ̈PkinA ƋΦϠǥ̓~Ǟƣyƥ̬|ѺМŎ
Ѐ|]d EGFP-CsmA ~ȥǏξɉ_ҥȖhqQqPɴȟЌ|Ьƣjqy{ǧξǔ¢Á¬â
EGFP-KipArigor y mDsRed-CsmA ƥ˞|ψΫl̓ĥҚjPòя~ȥǏԌĝ~͈ӡҎ{tqyfPѺМŎЀԌƕѺМŎЀ^ɮˎ~ѺМŚ|]ZwPEGFP-KipArigor ~ȥǏlɴȟЌë| mDsRed-CsmA~ȥǏ_ҥȖhqQqPCsmA-HA y EGFP-KipArigor ƥ˞|ψΫl̓ĥҚjPʞ HA ʞĢγZq
Őπ͒԰Ȋգ|òя~Σέϋϓăĥγ|uZw̥ҫjqЬ̈Pòя~ϓăĥγ_ϥhqQf~Ь̈^PCsmA ~ѺМŎЀ~ȥǏƂ| KinA rdx{b KipA ԫîlfy_ϥƷhqQ1) ȝȶP˒˷ӧѯƂȀė 2010 ɍɗǫėҽ΄Ҟ˓ՀPp. 198
The roles of kinesins in the localization of chitin synthase CsmA in Aspergillus nidulans
Makusu Tsuizaki, Hiroyuki Horiuchi, and Akinori Ohta. (Dept. of Biotechnol., Univ. of Tokyo)

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МΥѺ Aspergillus nidulans |]d Saccharomyces cerevisiae CRH Þ²à¥~̴њҧ̅ϣ˼Ɏ͘նȝȶϖ̦նǙŚҘùնǭδ˘ɵ մ̂ĉǫäӧäαƂȾյԒ͆նМΥѺЇ~ϖ̔ɴαΣ~ЦјғȫЦјǤ|twҟw]նЦјǤ¥Þ âն¢¶âնÒ
â¾âЇöҞ̫ʒʒŤyjwZQf~ЦјǤ̫ʒʒŤ~[sնԒ͆x¢¶â~ƍųƣ_ 12%ϳɗxY~|ȘjնМΥѺx 10-30%ϳɗxYfy_ϛw]նМΥѺ|]dЦјǤ¢¶â~ƣʒնŤҧմÝÖ»Ýâ¥յԒ͆ƑȒ|ūɱhwZyĀʊhQ
S. cerevisiae |]ZwնЦјǤ¢¶â~ÝÖ»Ýâ¥|̴њlǆǼ|uZwҧ̅hw]նo~[s¢¶ây¥Þ ây~̳̋|̴њlКӞϱԒУ Crh (Crh1pնCrh2p)_Ӫɍ˘^y{tqQçˎնA.
nidulans ~§ÂÔô|]Zw 5 ķ~ CRH Þ²à¥(crhAնcrhBնcrhCնcrhDնcrhE)_ǾǏlfy_˘^y{twZ_նo~̴њ˵ҧ˘~xYQ
ČǅնA. nidulans A1149(ΔnkuA)̓Ҥ̓yjw 5 ķ~ CRH Þ²à¥opƋΦϠǥjq̓ĥҚjնo~ɿӔ~̥ҫҎtqyfնΔcrhAնΔcrhBնΔcrhCնΔcrhE ̓|]ZwՕҀ{αіӹɝɦʏξɉҠ{^tqQçˎնΔcrhD ̓|]ZwՕҀ{ɦʏξɉҠ{^tq~~նαіӹɝ_Ҡն¨à¿åϒɬԙαǔ̓~ 76%ϳɗxYtqQf~fy^ A. nidulans |]ZwfԄĘǼ_Ԙқjq̴њʧufy_ʱȉhqQΫǏնcrh ԄĘǼ~ǪԘϠǥ̓~ĥҚЇӷwZQ
Functional analyses of CRH orthologs of Saccharomyces cerevisiae in Aspergillus nidulans.
Koji Isomura, Makusu Tsuizaki, Hiroyuki Horiuchi, and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)

P-34
МΥѺ Aspergillus nidulans |]dţѰԒ͆ Saccharomyces cerevisiae SKT5 Þ²à¥ AN3445 ~̴њҧ̅˚ ͨѣնȝȶϖ̦նǙŚҘùնǭδ˘ɵմ̂ǫԲäӧαϯäɼαȾյSaccharomyce cerevisiae |]Zwնdivision 2 |Ȫl¤Ü®G¢¶âƣʒԒУ Chs3p նЦјǤ¢¶âαƣʒ~ǫԌŤʤtwZQChs3p ~ūɱªÊÙ¿¸¼xY Skt5p նţѰɦʒ˞| Chs3p  bud neck ~ɦӔѡë|Ý¤Þå¼lfyնChs3p ~ͥɿƂ|ԫîlfy_ϥƷhwZQAspergillus nidulans |]Zw division 2 |Ȫl¤Ü®HƕI¢¶âƣʒԒУ CsmA ƕ CsmB նѺМŎЀƕԹǤɦʒԌĝ|ȥǏjwѺМǔ~ɦʏɦʒ|ԘҞ{ɫų̈qlQA. nidulans | SKT5 ~Þ²à¥yjw 3 u~ԄĘǼ_ǾǏlQɥϟϹȋ|]dfx~ҧ̅~Ь̈նo~ô~ AN3445 ~ԄĘǼϠǥ̓|]Zw~ԙ
αǔ̓y͈ӡjwѺМ~ǪŤȭնЦјǤ̫ӵ~ξɉЇ_ҥȖhqQq EGFP-AN3445  CsmA YZCsmB yƥ̬|ѺМŎЀƕԹǤɦʒԌĝ|ȥǏ_ҥȖhնAN3445 _ division 2 ¢¶âƣʒԒУ~̴њū
ɱ|ԫîlƞњɿ_ю\wZ 1)QČǅնAN3445 ~Ϡǥ̓|]Zw EGFP-CsmA ~ѺМŎЀ|]dȥǏҥȖjqЬ̈նԙαǔ̓y͈ӡjwѺМŎЀĐӪ~ɦӔѡë|]dȥǏ~ųƣ_ĞìjնЦјӔô|]d½¸¼Υ~ȥǏЇ_եՒɗxҥȖhqQçˎնCsmA-FLAG ƕ EGFP-AN3445 ƥ˞|αβl̓~ЦјʣţͭγZwŐπ͒԰͜| CsmA y AN3445 y~ϓăĥγ~ˮΖ̥ҫjqyfնòяԪ|ϓăĥγϢҴh{^tqQf~Ь̈^նAN3445 ~ϠǥѺМŎЀ|]d CsmA ~ȥǏ|çԌɪՍî\~~նo~ɪՍԪʰϋxYfy_ϥƷhqQ1) ҝţն˒˷ӧѯƂȀė 2010 ɍɗǫėҽ΄Ҟ˓Հնp. 212
Functional analysis of a Saccharomyces cerevisiae SKT5 ortholog in Aspergillus nidulans
Hiroomi Hoshi, Makusu Tsuizaki, Hiroyuki Horiuchi, and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)

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լѺ A. oryzae |]dԃʡϋå¼É­åԫӶԄĘǼ Aoatg11 ~ҧ̅δʖԶùնѹԪԶɻնƃ˷žf մ̂ǫԲäӧαϯäɼαȾյ
VϑϋWå¼É­åնЦјӔʒŤԹՂѡxǉZөfy|twå¼É©²åÔɦʒjն
Ý²²åÔ/ͭјxŤҧl̴̫xYնϖ̔αΣ|ɑbĳǾhwZQӪɍնԒ͆|]ZwΤȉ~´âÅ¤ӔÞ¡ÁÜΤξϋ|Ҵҿjնͭјӥӯlԃʡϋå¼É­å~ǾǏ_ϢҴhwZQj^jնA. oryzae {zМΥѺxo~ǾǏϢҴhwZ{ZQå¼É­åԫӶ´âÅ¤Ӕ~ôxԃʡϋå¼É­åΤξϋ|̴њl´âÅ¤Ӕ_Ҡu^tw]նԒ͆ S. cerevisiae ~ Atg11p ԃʡϋ|ҴҿjqǘӔ~å¼É©²åÔɦʒ~Ǟ~ӥӯ|̴њlfy_ǝƭhwZQ˷ϟϹxնA. oryzae|]dԃʡϋå¼É­å~ǾǏ˘^|lfyϑϋyjնo~ÏÖà¥xY AoAtg11 ~̴њҧ̅ҎtqQVˎ͜äЬ̈WAoatg11 ԄĘǼϠǥ̓ĥҚjնˬȠǗǑ|]dαіҥȖjqyfնαі~ɀնfxҧ̅hwZå¼É­å̶ʷ̓|Τɶϋ{͊ôѺМ]ŤαǼ~ɦʒԮȍҠ{^tqQqնA. oryzae |]då¼É­å~Òå å´âÅ¤ӔxY AoAtg8 ~ EGFP Ҍƣ´âÅ¤ӔϠǥ̓|ψΫhnqyfնԙἀyƥ̬նͭј~҂ϸ_ҥȖhնՊԃʡϋå¼É­å̺ɉ|̴њjwZfy_Ť^tqQf~Ь̈ն AoAtg11 Պԃʡϋå¼É­åx{bնԃʡϋå¼É­å|̴њl´âÅ¤ӔxYfy_ϥƷhqQΫǏնAoatg11 Ϡǥ̓|]Zwնԃʡϋ
å¼É­å~ǘӔxYÓÂÎË¶µå±EմAoApe1յÓ¼¨â½ÝնÎÞ¢¬²åÔն̔~ͭј~ƚөҥȖôxYնԃʡϋå¼É­å|]d AoAtg11 ~̴њҧ̅ӷwZQ
Analysis of Aoatg11 related to selective autophagy in Aspergillus oryzae
Takayuki Tadokoro, Takashi Kikuma, Katsuhiko Kitamoto (Dept. of Biotechnol., Univ. of Tokyo)

P-36
Magnaporthe oryzae ~ŤƂ̬ɞyå¼É­å̴̫|ΣӔđҾ~ԫӶɿąëŷǲǼ 1) 3)նϑկСǼ 1)նř̃ѓǼ 2) 3)ն˸˽ŋ 1) 3)ն͐δĽç 1) 3)
մϪʕǫԲ 1)ä̲̈ϟ 2յäαϟ°â´å3)յ
Magnaporthe oryzae նψѰӽϳ~ΰǢ˿Ĕ|twξ{ŤƂ̬ɞyQʰǑՋ_ο͋ɿ~ǞƣնĐϘǂŤƂhn_նҤ͋ɿxψѰЌěԧjдdQfx~ϟϹ^նĐϘǂŤƂhnqјǼxնå¼É­åčjwβαhqÁÞ£åͽŪγjwʒΞjqĐϘǂ_ɦʒhfy_ŤǼαΣȀϋ|ϥhw`qQj^j{_P˵rјǼŚ~å¼É­åȟј~Ȋʏʬ\|ѦtwZ{ZQʓRնɿӔ~ξ{ʰǑՋxψѰhnqјǼ~ӘɴЦ̫ӵyŤƂ|Ě[ӎ҃ΣӔ~Ż`ҧ̅jqQĐϘǂɦʒlο͋Ջëx 12 ˞ԪǗ՜jqјǼxնͭј_ҌƣäѢǫƂjնo~ŚԌ|ћѕ΃Їƪå¼É­å|Τɶϋ{ĂԘѡȟј_ҥȖhqQqP24 ˞ԪǗ՜jqјǼxնyz~Цјȟ

ǂȇ_ͬǰjwZqQçˎնĐϘǂɦʒj{ZҤ͋Ջë~јǼxնͭјŚ|ĂԘѡȟјҥȖhmնЦјȟǂȇĳʧhնˍq|ԹǤ Woronin ȟĢ_ɦʒhwZqQqնјǼ~ӎ҃ÁÞ£åͽ~ӞӯyzҴ{^tqQf~Ь̈նĐϘǂŤƂhnΰǢ˿Ĕì~јǼxնå¼É

­å̴̫|ӎ҃ÁÞ£å~đҾyնo~Ӟӯ_ıhfyɤbʽʧjwZQ
Differentiation in Magnaporthe oryzae regulated metabolism in conidia via autophagy
Kanako Inoue1) 3), Satoko Kanematsu2) 3), Pyoyun Park2) 3), Kenichi Ikeda2) 3)(Kobe Univ.1), Natl. Inst. Fruit Tree Sci.2), Bio-oriented Tech. Res. Adv. Inst.3) )

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ʤǼѺ¬¥²Æ¼Û´¦|]då¼É­åԫӶ´âÅ¤Ӕ CcAtg8 ~ҧ̅͹ԇ ɩնɡαӏҘնƤ˼˗ɵնխδʂɧմ՟Ⱥǫäӧäɼαϯյå¼É­åyնĂԘѡ̫ӵƚå¼É©²åÔčjqǫҡ̯{ЦјŚŤҧ¬®ºÔxYQӪɍնƢϵϖ̔αΣ|]då¼É­å~ҧ̅^նå¼É­å_̑՜ՙ՝|ȘlԂɼrdx{bնЦјŚ´âÅ¤ӔÞ¡ÁÜ~ƳӔЌέնЦјŤƂնʎ̍ЦѺ~Գƒ{zն̬R{αƱΫӅ|ԫîlfy_˘^y{tw`qQ˷ϟϹxնÖ»ÞʤǼѺ¢Â¨xY¬¥²Æ¼Û´¦մCoprinopsis cinereaյ|]då¼É
­å~ɫųuZw˘^ylqնå¼É­å~ӷҎ|y{Zͭј|ƚөå¼É©²åÔЬƣ´âÅ¤Ӕ CcAtg8 |Ϙϑjնҧ̅ҎtqQfx~yfնлѬҋŏ´âÅ¤ӔҌƣhn
q CcAtg8 ~ȥǏҧ̅^ն¬¥²Æ¼Û´¦|]ZwϽУͽՙ՝˿Ĕì]ÜÅÒ¬âǾǏì|]Zwնå¼É­å_ҵȞhfyϥƷjwZQČǅնh|å¼É­å~ҵȞ˿Ĕ|uZwʯФӷqЬ̈նëҬ˿Ĕ~ďնǗǑô~ EDTA ʹŷ˿Ĕì|]Zwնͭјô~лѬҋŏ~҂ϸ_ҥȖhնƥ˿Ĕ|]Zwå¼É­å_ҵȞhfy_ϥƷhqQqն¬¥²Æ¼Û´¦|]d CcAtg8 ~αΣȀϋ̴њ˘^ylqնCcatg8 ~Â¸¤µâҧ̅|uZw̥ҫjqQЦјŚx dsRNA βαl[{ RNAi ҵȞÍ¤´å̫АjնÒååԄĘǼyŕ|ŕɦӔӞʴ͜|ն¬¥²Æ¼Û´¦̍ѬĢô|ȞőjqQo~Ь̈նɰqψΫʜū̓նҤ̓|͈yӊɢ{ѺМαіϥjqQΫǏնɰq̓~ҼɿӔ|uZwh|ҧ̅ӷwZQ
Analysis of CcAtg8 involved in autophagy of Coprinopsis cinerea
Akira Watanabe, Takahiro Yayoi, Masanori Yoshimura, Yasuhiko Asada(Dept. of Appl. Biol. Sci., Fac. of Agr., Univ. of Kagawa)P-38
ʤǼѺ Coprinopsis cinerea ~´âÅ¤ӔŤ͛~Ó¬â~ԫî̳˷ɑϨ 1ն̰δʇԾ 2, ˍŐӤζ 2, ƤδҶ 1 մ1 ̂ĉӧȾǫäӧ, 2 ŖəϕǫäαƱέյfx|΄я~¥ÞåËxʤǼѺ Coprinopsis cinerea _αβl̤ΣЦјǤǪКՖŤҧԒУ~ԒУȀϋ̴њ|uZwϟϹҎtw`qQf~ԒУ~Ť̴̫͛|ԫlϛҠ˷Ѻ~̤ΣЦјǤŤҧ̴̫έҧlëxԘҞxY_o~ҳЦí˘xYQŻΣԒ͆{zxնŤ͛|ԫîlǆǼ~çuyjw¤¶âНÖå´å´âÅ¤ӔxYÓ¬â_ΤȉhwZfy^ն΄я˷Ѻ~´âÅ¤ӔŤ͛|]ZwÓ¬â_ԫîjwZ~x{Z^yю\qQofx˷ϟϹxC. cinerea ϟϹȘӅyjwնÓ¬âͥɿԮȍŰ~ʹŷ_˷Ѻ~´âÅ¤ӔŤ͛|î\ɪՍ|uZwҺ̐jqQq C.
cinerea xψΫjwZÓ¬â~ӻŻͥɿЦјŚȥǏ|uZwҧ̅ҎtqQ

Involvement of myosins in protein secretion by basidiomycete, Coprinopsis cinerea
Kohsuke Hashimoto1, Etsuo Yokota2, Teruo Shimmen2, Makoto Yoshda1(1Fac. of Agri., Tokyo Univ. of Agri. & Tech., 2Grad. Sch., Life Sci., Univ. Hyogo)

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ʤǼѺ¬¥²Æ¼Û´¦~Łʒԧ|ԫԄĘǼ cag1 ~ҧ̅˼Ɯ ŌնΚȬƲ̲ մϮδϕϾǫäαΣӒͽյʤǼѺ¬¥²Æ¼Û´¦~ǼȊĢɦʒӽϳ|]ZwPǼȊĢƐǘ~ŁԌŤ_ʒԧnm|ǼȊĢƐǘ~Υʏx̹twj[ϼΘǧξĢҠţjqQǼȊĢƐǘ~пˌťΡĥtwЪфҥȖjqyfPŁԌŤ_ϝϼëxѢjwZ~~PǼȊȫ_ŤƂjwZ{Z[|ɾqQo~ϼΘǧξĢԙαǔ
̓yćԎjPF1 Ǽȁ|]dғΫǔ~ŤՂ͈ҺqyfPԙαǔրϼΘǧξĢց47ր65մպրպP>ջ
ց2.89, P>0.05յ|ŤՂjq~xPպԄĘǼʽԎ~ɦӔxYfy_ϥƷhqQofxPƐǆԄĘǼ capgrowthless1 (cag1) yƦĐdPcag1 ԄĘǼɘ~Ò¸Èâ¥ҎtqQF1 Ǽȁ^ԙαǔ̓ջչ̓yϼΘǧξ̓ջչ̓ԃP§ÂÔ DNA ʣţjwԠǔyjPRAPD PCR ҎZPғΫǔyӶԥl RAPD Òå åʯjqyfPЅ IX ̍ѬĢë~ G13-900B Òå å_Ъʴ\Ϊ 15%xӶԥjwZfyҠţjqQԙαǔ cag1 ԄĘǼΤȉlq~ɦӔӞʴƛȏѺ̓#58 (cag1-1 trp1-1, 1-6) ĥPG13-900B Òå åӪĿ|ὲl BAC DNAմtrp1+ʧuյȞőjwҎtqyfPBAC ¤àåâ 7H8 ] 1F2 _ϓҙͥɿ(cag1+)
ʧufyϼ`̹qQ7H8 ~ DNA ūԱԒУ HindIII xԌŤŤҧjP°ÞÉÜ§å¼jwªÊ¤àåâĥPϓҙͥɿҺqyfPªÊ¤àåâ B4 _ϓҙͥɿʧtwZqQB4 ~ DNA  HindIII xťˌjw^ɦӔӞʴȊգ|γZwϓҙͥɿ̀twZqQofxPB4 ~ HindIII ˌΡop¤àå¿â¥jwPz~ˌΡ|ԙαǔ cag1 ԄĘǼ_ûtwZ~^ҺwZyfxYQ
Analysis of the cag1 gene, which is involved in cap growth of Coprinopsis cinerea
Hajime Muraguchi, Kazuki Kemuriyama(Dept. of Biotechnology, Akita Prefectural Univ.)

P-40 (O-6)΢ѶŕαМΥѺ Epichloë festucae ~ĞŤǼԚ G ´âÅ¤Ӕ Cdc42 ƕ RacA ~̴њŤƂ|ԫlϟϹ̩ԙƖ՟նЂ˷ǫƬմƦǫԲäαƱӧȀյE. festucae Áϯ΢Ѷ~ЦјԪԺxαіjնŕαԫİϢϾlМΥѺâ½É¼xYQfx|նE. festucae ~ͥɿԓУαʒԒУ NoxA _ŕα˞~̤ΣŚѺМ~αіūɱlfyնūɱǆǼ NoxR yĞŤǼԚ G ´âÅ¤Ӕ RacA _նNoxA |ͥɿԓУαʒ̺|ūɱlfy_ҧtwZQqԒ͆xЦј̨ɿ~ϢϾ|ԫîl BemA/Cdc24 _նNoxR yқƣĢɦʒjͥɿԓУαʒūɱlƞњɿ_ϥhwZQČǅնRacA ] RacA yեZϓƥɿϥl Cdc42 ~̴њ͈ӡҧ̅jqQYeast two hybrid ͜γZq Nox ūɱǆǼy~Ьƣҧ̅|նRacA  NoxR y Cdc42  BemA y N ˶Ѐľ~ÓÂԓčjwΤξϋ|Ьƣlfy_ϥhqQracA Ϡǥ̓xնѺМŤȭ_ǣŷl~|Șjնcdc42 Ϡǥ̓xԌŤϋ{ѺМЦј~Ѣɣ_ҴqQq racA Ϡǥ̓նȐö̤ΣŚxξɉ{ѺМαіϥjw̤Σ̊̾hn~|Șjնcdc42 Ϡǥ̓Ȑö̤ΣyŕαԫİϢϾx`qQcdc42 Ϡǥ̓xԙἀy͈ӡjwͥɿԓУαʒԚ_ǣŷjqfy^նCdc42 _ͥɿԓУαʒ~Ӈ~ūɱǆǼxYfy_ϥhqQf~Ь̈նCdc42 y RacA _Ёƣϋ|Ň`նͥɿԓУαʒѺМαіūɱjwZƞњɿ_ϥƷhqQ
Functional analysis of small G proteins Cdc42 and RacA from fungal endophyte Epichloë festucae
Yuka Kayano, Daigo Takemoto(Grad. School Bioagr. Sci., Nagoya Univ.)

58

P-41
ƯǁγĞÏ®É´å±լѺ~ДլնӃլ|]dÏ®É´å±ԄĘǼψΫõąͱç˱ 1նǪδŶα 1նϬȮϖԗ 1նԜ˴ ѓ 1ն˰ԌՑǭ 1նƲø ӄ 2նϊϝ͢Ɍ 2նƃ˷ŭҎ 3ն˺˷ӿƴ 4ն̦˷ʑç 1 մ1 ӧϟ̴̫ä՘кϟն2 Ç¸¤ն3 ʍϛβʚϟä՘Ⱦʚ°ն4 ¾ Öյ ҺƯƯǁմrjőƯǁյ~Қӵ|γZÂ¬âԓ{z~̔ԓН˓ƯʒŤնլѺ|ὲlƯǁ
ô~Ï®É´å±|Ťҧhfy_ϛwZQo~Ťҧԭ̹|ŷΟ|ԒУ~ǰͥŢέ_ɹҞyh_նեͺŷΟŢέƯǁ~ƳӔĞì|u{_fy^նѺĢǩÏ®É´å±ͥɿ~ĞZլѺȊγ̓_͍wZQʓRլѺ~Ï®É´å±ψΫūɱ̴̫̺jbέҧjնƯǁγլ~Ï®
É´å±ͥɿĞ͸|ɼγlfyϑʨjwZQ ˷ϟϹxնДլնӃլx~ԓɿÏ®É´å±ͥɿ_ĞZƯǁγլѺyjw Aspergillus oryzae KBN8048
̓ 503 ϵՖ~լѺ̓~ô^ԃʠjqQKBN8048 ̓~ΤɶyjwնԓɿÏ®É´å±ͥɿ_ӃլxΤ|ĞbնÂ¬âԓŤҧͥɿДլĞZfy_ʩeQƥ̓~ДլնpH _ 5.0 ĐӪxնÝâԓͽ_ӃլȠ{Zfy^նԓɿÏ®É´å±_ҵȞhlZyю\qQȉԚ RT-PCR ~Ь̈^նƥ̓~ 13 ϵ~ѺĢǩԓɿÏ®É´å±ԄĘǼӞŞԚ_ДլxǪZ type R yӃլxǪZ type S |ŤՖx`qQtype R ԄĘǼщyo~ūɱ̴̫Τ|Дլ~Ï®É´å±ͥɿĞ͸~q~̮ϋyjwˮ˲
xYQçˎxնtype S ԄĘǼщ~ψΫ|ɪՍl pHնÝâԓĒǩ~ǆǼ~ǾǏϥƷhqQ˷ϟϹTˍq{ӧ̇͋βˀЊʱӷlȊγʚҏԩψā̧U~çΰyjwȊˏjqQTranscriptional profiles of the acid phosphatase genes in A. oryzae low acid phosphatase activity strain in solidstate rice and soybean cultures for miso brewing
Junichiro Marui1, Sawaki Tada1, Mari Fukuoka1, Satoshi Suzuki1, Ryota Hattori1, Yutaka Wagu2, Yohei Shiraishi2,
Noriyuki Kitamoto3, Tatsuya Sugimoto, Ken-Ich Kusumoto1(1NFRI, 2Bio’c, 3Food Res. Center, Aichi Ind. Tech. Inst., 4Nakamo)

P-42ƯǁγլѺ A. oryzae KBN630 ̓|]dԓɿÏ®É´å±(Aph)ԄĘǼщ~ϠǥȄδմƤԙյɒǼնԧӂȺ ʸնȟԙǲͤǼնĖӑ Ħш 1նϊϝ ͢Ɍ 1նƲø ӄ 1ն˺˷ ӿƴ 2ն ̦˷ ʑ
ç 3նƃ˷ ŭҎմʍϛβʚϟä՘Ⱦʚ°ն1 æÇ¸¤ն2 ¾

Öæն3 ՘кϟյ

VϑϋW̔ԓНҺƯˋʹŷjqҺƯƯǁնƯǁô~լѺÏ®É´å±մAphյ|˓ƯʒŤ~Ťҧ
ԭ̹~qնեͺ~ŷΟǰͥŢέ_íƞ̶xYQj^jնեͺŷΟ|Ưǁ~ƳӔ_ĞìlqնAph ĞαβլѺŪγjqˍҡ{ϔÁÞ£åǔƯǁҚӵʚҏ~ԩψ_͍wZQ˷ϟϹxնƯǁγլѺ|]dեՒɗϓƥЪʴ\Н~ԩψy aph ԄĘǼϠǥ̓~ĥţҎZնƯǁҚӵ|]d aph ԄĘǼщ~̴њҧ˘y Aph ĞαβլѺ~іϵϑʨlQVˎ͜äЬ̈Wfx|նƯǁγլѺ A. oryzae KBN630 ̓|]Zwնku70äpyrG ԄĘǼĂԘϠǥ̓ A.
oryzae KBN630-17K3 ̓ƚɰj 90ճĒë~եՒɗϓƥЪʴ\НԩψjqQ̷|լѺ§ÂÔʉǝ^ A.
niger phyA ԄĘǼyϓƥɿ~YԄĘǼ 8 ķմaphA;aphH ԄĘǼյնA. niger phoA ԄĘǼƕ A. niger aphA
ԄĘǼyϓƥɿ_եZʱȉԓɿÏ®É´å±ԄĘǼop 3 ķƕ 2 ķմaphI ԄĘǼքaphM ԄĘǼյҠţjնop~ԄĘǼϠǥҎtqQ13 ϵՖ~ԄĘǼϠǥ̓γZwӃլĥҚjնԓɿÏ®É´å±ͥɿ]ҺƯˋ~Â¬âԓŤҧͥɿͻȉjqЬ̈նaphC ԄĘǼϠǥ̓|]ZwòͥɿyՕҀ|ĞìjwZqQ{]˷ϟϹTˍq{ӧ̇͋βˀЊʱӷlȊγʚҏԩψā̧U~çΰyjwȊˏjqQ
Disruption of the acid phosphatase (Aph) genes in the miso koji mold, A. oryzae KBN630
Shoko YOSHINO-YASUDA, Osamu HASEGAWA, Natsuko ONO, Yoshimi IGA1, Yohei SHIRAISHI1, Yutaka
WAGU1, Tatsuya SUGIMOTO2, Ken-Ichi KUSUMOTO3, Noriyuki KITAMOTO ( Food Res. Center, Aichi Ind. Tech.
Inst., 1 Bio’c CO.,LTD, 2 NAKAMO CO.,LTD, 3 Nat’l Food Res. Inst. )59

P-43 (O-19)
ԄĘǼǪԘϠǥ| Aspergillus oryzae ~ Hydrophobin щ~̴њҧ̅ȬȺ Ьն͋ԙġ՟նôȴ˛Хմ˘͘ǫäӧäӧƂյHydrophobinմÃ½àÉåÇâյyϺ͊ô|ѺМěԧhnǼǄѺäʤǼѺՖ|Цјғȫ´âÅ¤ӔxYQĞŤǼԚմ70~150a.a.յPòҤǻɿyZ[ΤɶϥlQÖÂÒåyjwѺĢǩ|Ť͛hqɮPҤ͋-ο͋ιՋ|]ZwѥɁՀƣjPѺМŤαǼ~ʹ͋ɿ~ĐîP̤ΣЦјғՋ{z~ο͋Ջ
~ƫϘ|ԫîlfy_ϛwZQçѪ|Pպu~Ѻ̓ăZ|ϓƥɿ~ĞZ Hydrophobin ԄĘǼқˆʖˮjwZ_Po~ĨZŤd|uZw yzҧ˘hwZ{ZQ˷ϟϹȋxլѺ Aspergillus oryzae |͟ϑjPHydrophobin ¨å½lyʱȉhԄĘǼ 4 uƋՂj
մhypA,B,C,DյPhypA,B,C |uZwψΫϢҴjwZQҋŏҌƣ´âÅ¤ӔγZqȥǏҧ̅|P
ŤαǼ| HypA j^ҥȖhmPçˎѺМx HypA,B,C ~Œw_ȥǏlfy_ϥhqQHypA y
HypB,C ~Ԫ~ȥǏɿ~ɀξ^ƥçѺ̓_ψΫl Hydrophobin ~̴њϋĨZŤd_ϥƷhwZQ˷ϟϹx hypA, B, C |uZwƋΦPĂԘ]êԘϠǥ̓ĥҚjնo~ғΫǔҧ̅^op~̴њҧ˘lfyϑϋyjwZQÝÞ´Ô PCR ҧ̅PɦʏҥȖPψѰΪͻȉPʹ͋ɿұգPSEM |ɴЦ̫ӵҧ̅~Ь̈PA. oryzae ~Ã½àÉåÇâщop~ψΫ˞˳]ȥǏķʖ_ξ{PoΦϾjw̴њjwZfy_ϥhqQqǪԘǧξ̓xPǗ՜˿Ĕ|ʹ͋ɿ_ϥhqfy^PӞŞԚ~ĞZ HypDPYZ Hydrophobin Ēǩ~ΣӔѺĢ~ʹ͋ɿ|ԫîlƞњɿ_ϥƷhqQ
Functional analysis of Hydrophobins by multiple gene disruption in Aspergilllus oryzae
Yui Yamakawa, Yuka mizuno, Harushi Nakajima(Dept. of Agricultural Chemistry, Univ. of Meiji)

P-44 (O-20)
͋˥ʪŻǼÒ¤àÄÜâ® (QCM) |ιՋͥɿҊϊӔ RolA yǋĢғՋԪ~ϓăĥγҧδԈɠͅ նǫՖˣǼ նëƐĽĂ նե̳ɷ նŠ̱ӏʒնˮδϴɧնԯԌ˅ʇ մ ̂ƃǫԲäαΣβ̧Ŵʒն ԐՖϟäǘϏն̂ƃǫä˵̀ϟն̂ƃǫäǪŌϟյVїˣäϑϋWլѺαŤҧɿÑÝ®ºÞxY PBSA մ Polybutylene succinate-co-adipate յƸç~ΓУͽyjwǗ՜lyն PBSA ŤҧԒУ~ cutinase CutL1 yŕ|ն hydrophobin RolA ψΫlQ
RolA ն CutL1  PBSA ғՋ|Ý¤Þå¼lāxն PBSA ŤҧŹΪë˖hnwZQçˎնhydrophobin çѪϋ|ǋĢғՋȅŒ|ҔѡjqΥʏxɤǋ|ЬƣlΤɶʧuQj^jն CutL1 ~PBSA ŤҧԮȍjwZ{Zā^ն RolA ƫϘɮ|͋ɌˎƧϱŻlƞњɿ_ϥƷhqQofxն͋ɌˎƧƞŻÕ ¿¯Ôҧ˘lΕ|ն RolA y PBSA Ԫ~ϓăĥγÕ ¿¯Ôҧ˘lāyjqQ
VЬ̈äюȖWƢ pH |]d PBSA ~ RolA ~ƫϘԚyƞŻɿҧ̅jqЬ̈նòя|ϓԫҠţn{ ^ t q Q h  | ն RolA ~ PBSA  ~ ʱ ȉ Ь ƣ Ց ǖ Ś ~ ǧ ξ Ģ մ L137S, L142S, D134A/D136A,
K130A/D134A/D136A յĥҚjնf|uZwƥ̬~ҧ̅ҎtqQo~Ь̈նԙαǔ RolA y͈ӡjwնǧξĢ PBSA ~ƫϘњ_ǧƂjq_նƞŻɿ|ǧƂ_Ҡ{^tqQf~ā^ն RolA
~ PBSA ~ƫϘyƞŻɿξ{Õ ¿¯Ô|ĬǾlΫӅxYā_ϥƷhqQh|ն RolA ~ƞŻɿ|նT PBSA ~ƂȀ̫ӵU]TΔǧξȞő|ǧƂjqĒë~ՉՅϋäο͋ϋ{ϓăĥ
γU_ǫ`bȑîlā_ʱһhqQofxΫǏնŤǼԪϓăĥγҧ̅Җц QCM ~°âªå¶¸ËғՋ|ο͋ɗǧ\qƂȀĵ՛ˏjwնo~ғՋ|Șl RolA ~ƫϘњҰĭjwZQ
1) Takahashi et al., Mol Microbiol. 57: 1780-1798 (2005)
QCM analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces
Hiroki Tanabe1, Keiko Orui 1, Kenji Uehara1, Toru Takahashi2, 3, Takanari Togashi4, Toshihiko Arita4, Keietsu Abe1, 2(1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NICHe., Tohoku Univ., 3 NRIB., 4 IMRAM., Tohoku Univ.)

60

P-45
Â¢´¦ὲ~ hydrophobinմFv-hyd3յҌƣ´âÅ¤Ӕ~αβƤδϖΊP҇δțɎ 1PҝȺѫɌ 1P϶Š ѓ 2PδƜʅ˱ 1Pìǒ Ҷ 1
մ1 ĴȻǫäтжäɼαНP2 Ï¤¼`~fкƣϟϹʖյ
Hydrophobin МΥѺ~Цјғȫ|ˢӼϋ|ǾǏlĞŤǼԚ~´âÅ¤ӔxPЦјǩxѥɁՀƣjòҤǻɿ~ѡɦʒlQçѪϋ|МΥѺ|қˆ~ hydrophobin ԄĘǼ_ǾǏjPƢR_ξ{tqɫųʧtwZQHydrophobin ~ö{ɫųyjw͊ôѺМɦʒPο͋ɿғՋ~ѺМ~ƫϘPјǼғՋ|]dο͋
ϋ{ЃΥ̫ӵ~ɦʒ{z_ʩeQfx|Â¢´¦§ÂÔÜÊÜÝå 2 u~ hydrophobin ԄĘǼ Fv-hyd1PFv-hyd3 ~ƋՂ|ʒŶjqQƈȉԚϋ RT-PCR ~Ь̈^PfopǼȊĢPѺМΤξϋ|ψΫjwZfy_˘^y{tqQf 2 u~ hydrophobin ԄĘǼ~ cDNA ŒԧԎŦPop´âÅ¤ӔψΫÍ¤´åpGEX 5X1 |ȞőjPE.coli Rosetta2(DE3)̓|ξϵȐöψΫҎ{ZPSDS-PAGE |ҧ̅jqyfPGSTyҌƣjq Fv-hyd3 ´âÅ¤Ӕմ37k Daյɰfy_x`qQΫǏPɰq Fv-hyd3 Ҍƣ´âÅ¤Ӕ~ǫԚαβұyy|PFv-hyd3 Ҍƣ´âÅ¤Ӕ~òҤǻɿ|uZwҰĭҎ{twZQ
Expression of hydrophobin (Fv-hyd3) fusion protein derived from Flammulina velutipes
Masumi Yoshida1, Masayuki Fujita1, Ryohei Nishikawa1, Satoshi Inatomi2, Goro Taguchi1, Makoto Shimosaka1( 1Division Appl.Bio., Fac.Tex.Sci.Shinshu Univ., 2Mushroom Lab.Hokuto Co. )P-46
ծլѺ KexA ~̴њҧ̅Šδ͔ђǼն̢δȕĊնŮδͨնЂŚӾԾնȬɦ͢Ɍմ̂ӧȾǫԲäɼαϯյ
VϑϋWծլѺ~§ÂÔ| serine-type carboxypeptidase ¨å½lyʱȉhԄĘǼ_ 12 ķǾǏlQf~[s~çu_ Saccharomyces cervisiae ~ KEX1 ԄĘǼ~ÏÖà¥xYfy_ÓÂԓԎŦ~ϓƥɿ^ʱȉhնf KexA yƦĐdqQ S.cerevisiae ~ Kex1p նÎË¶½~ C ˶Ѐ^
Lys,Arg ӺՂl exo ǔËàºå±xYնʰƣÉàÖâ~Ëà°¸¬â¥Цј̾|ԫîjwZ
Q˷ϟϹxծլѺ|]d KexA ~̴њҧ̅lfyϑϋyjqQVˎ͜ƕЬ̈W KexA ~̶ʷ̓նӽŲψΫ̓ĥҚjնo~ғΫǔ¨â¼àåÞ̓y͈ӡjqQo~Ь̈ն̶ʷ̓xŤαǼɦʒњ~Ğì_ҴqQçˎնӽŲψΫ̓xѺМ~ǪŤȭќɢƂ_ҥȖhն Calcofluor White ʹŷjqǗǑ|]ZwҀjZʒіԮȍ_ҴqQĒë~[{ғΫǔЦјǤξɉ_ʋӗhq̓xbҴfy^ն KexA ծլѺ|]ZwЦјǤԫӶ´âÅ¤Ӕ~Ëà°¸¬â¥|ԫîjwZyю\qQ{]˷ϟϹαϟ°â´åǘϏϟϹʱӷā̧~çΰyjwҎq~xYQ

Functional analysis of KexA in Aspergillus oryzae
Sayako Tomita, Hiroto Morita, Hirosi Maeda, Mitio Takeuti, Youhei Yamagata(Dept. of Applied Biological Science, Tokyo univ. of Agriculture and Technology)

61

P-47
լѺ serine-type carboxypeptidase ~ Aspergillus nidulans |]dեψΫ̓~̥ҫĠԙƲОն̢δȕĊնŮδͨնȬɦ͢ɌնЂŚӾԾ մ̂ӧȾǫäɼαƂյլѺAspergillus oryzae ~§ÂÔԎŦҧ̅~Ь̈ն12 ķ~ serine-type carboxypeptidases (CPase) ~ǾǏ_˘^y{tqQf 12 ķ~ CPase ԄĘǼնAspergillus nidulans ȐöyjwξϵψΫұqQo~Ь̈նեŤ͛~~yĞŤ͛~~_ǾǏjqQԎŦҧ̅|նf~ϓԀԩǷÕ¶¿âĒì 5 ÓÂԓ|YyĀͻhqQ˷ϟϹxնĞŤ͛ CPase xY TA13 ~ԩǷÕ¶¿âĒì 5 ÓÂԓեŤ͛ CPase xY TA4 ~~|цʴjq CPase ǧξԄĘǼնÓÜå±ËàÖå´åìͦ|Ьƣj A.
nidulans A89 ̓ɦӔӞʴjqQɦӔӞʴĢª«âÊà¸¼ҧ̅ÝÞ´Ô PCR |ҧ̅jqЬ̈նԩǷÕ¶¿âĒì 5 ÓÂԓцʴɦӔӞʴ̓ն5 ÓÂԓцʴjwZ{Z TA13 ɦӔӞʴ̓y͈w
եZ¨ÈåˆˮlŅƧ_ҴqQե¨Èåˆ~ɦӔӞʴĢΓУͽ®´å¶yjqҵȞ˿Ĕx 5 ˒Ԫ~ͭĢǗ՜]f{ZնǗ՜ë͵~ CPase ͥɿͻȉնSDS-PAGE ]®´âÊà¸ºâ¥ҧ̅
]f{tqyfնǗ՜ë͵~ CPase Ť͛ë˖jwZfy_Ũ˘jqQSerine-type carboxypeptidase from Aspergillus oryzae hyper expression mutants in Aspergillus nidulans
Kazunori Sano, Hiroto Morrita, Hiroshi Maeda, Yohei Yamagata, Michio Takeuchi(Dept. of Applied Biological Science, Tokyo Univ. of Agriculture and Technology)P-48լѺὲˍҡ β?¥Þ¨¬µå±|Τɶϋ{ǘӔΤξɿȾ҇ĦԉǼն͉ȎʒԶն͹Ԍ˝նˍӂȡɠնĄƯžýմ̂ƃǫԲӧäαΣβ̧ŴʒյʓRլѺ§ÂÔ»å´Íå®y|ĥҚjq β¥Þ¨¬µå±ԄĘǼ~ичϋեψΫ̓^նˍҡ{ β¥Þ¨¬µå±ЙҚjwԒУȀϋҼɿӔ~ҧ˘ҎtwZ(1)Qo~[s BglA  GH family 3 |Ȫjw]նƥÉÓÝå|Ȫlˑϛ β¥Þ¨¬µå±yՖĜjqƘɼѦԂ˿ĔЇϥjqQj^jƣʒǘӔ p-nitrophenyl-β-D-glucopyranoside (pNPG)|Șjw̨wեZŤҧͥɿϥl|^^mնçѪϋ{ β¥Þ¨¬µå±~ǘӔxY°àÇå®yzŤҧj{ZyZ[Τɶ_ҠţhqQf~fy^ BglA °àÇå®x{b pNPG y̫ӵϋՖĜɿʧuԎКĢ|ȘjwեZŤҧͥɿˮlƞњ
ɿ_ю\նȊԻ|ǫӃ²ÉÜÐâ|ȘlŤҧͥɿ̥ţlfy_x`qQǫӃ²ÉÜÐâ~Ťҧͥɿˮl β¥Þ¨¬µå±yjwլѺὲ~ BGL1 _ˑ|ǝƭhw](2)նBGL1 |uZwЙҚjҧ̅jqЬ̈ BglA yƥ̬| pNPG |ȘjwեZŤҧͥɿϥjqQqնBglA |uZwԄĘǼ~ψΫËàÉÞҺqyfնѨƯͰZfy|°àÇå®xҵȞ_ӗf{Zçˎx²ÉÜÐâ|twψΫҵȞhfy_ϥƷhqQ(1)Ⱦ҇ĦԉǼ^րЅǅМΥѺŤǼαΣȀ¨âÉßâ®Ҟ˓Հնp. 31

(2)Kaya. et al., Appl Microbiol Biotechnol (2008) 79:51-60
The Unique Substrate Specificity of Novel β-Glucosidases from Aspergillus oryzae
Kanako Kudo, Seiryu Ujiie, Akira Watanabe, Takahiro Sintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.)

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Ъʴ\լѺαβ°ÞÜå±γZqɑѿ̲¤ÜÉ¼ÅÞË~ԒУКƂƤ̑ Ĳϭ 1, Ԑą ыơ 1, Ȯղ ˉш 2, Ѹԙ ƇϮ 1, δô ź 2, øδ ƌŌ 3, ѽƐ ěƴ 4, ϰ ͢Ă 3,
Ӫ҇ ˝ɧ 1 մ1 ϪʕǫԲäȾäɼƂն2 ϪʕǫäŎЀҌƣϟϹΰն3 ˭̘şäкϟն4 ˒˷ТÅÞËϟϹʖյ
VϑϋWӪɍնƂϝΠˋ~̊ͶΰǢƺՔ{z~ҥΔ^նǑάë|ӄȓ|ǾǏjն՘ЛyЁƣj{Z˴ӔНÄÒ®^ΠˋƂȀƳαβlÄÝÉ¾Ýå~˳ɭ_եtwZQʓRնÄÒ®ƐˋyjwնѝÝ¥¿âŢέЇ~ŮŢέ_ˏhw]նˑǾ~ҭłγZwǫԚīаƞњxYÅÞË|ϘϑjwZQ˷ϟϹxնЪʴ\լѺxαβjq°ÞÜå±γZqÅÞË~ԒУКƂ˿Ĕ~̥ҫ
ҎtqQ
Vˎ͜Wâ½¥Þ ¾å±մEGյն°àÇÆ½àÜå± I ] IIմCBH I, CBH IIյն=¥Þ¨¬µå±մBGLյЪʴ\լѺ|Ť͛αβjqQɑѿ̲ˠ¤ÜÉ¼ÅÞËմLBKPյ|նαβjq°ÞÜå±ϵR~ͳƣ͈ΪxĥγhnնӺՂjq¥Þ¨å®Ԛ|КƂŹΪҰĭjqQ
VЬ̈WЪʴ\լѺxαβjq EGնCBH IնBGL Ԃť{͈Ϊxĥγhnfy|նLBKP _ŹΪϋ|ԒУКƂhqQh|նԒ͆γZqƥ˞КƂψԒ|նLBKP ^~´ÂåÞαβ|ʒŶjqQÄÒ®~КƂ]ψԒËà°®ƥ˞|Ҏ[бƣÄËà°®մConsolidated BioProcessingն
CBPյնËà°®ВμƂ|ϔÁÞ£åƂ~q~ԘҞ{ʚҏxYQΫǏնʓRնŊq´âÅ¤ӔäĂ̷đҾβΣαβ]ÄÒ®ӒƂњŵˮlլѺ| CBP ~ԩψҎtwZQ
Enzymatic saccharification of kraft pulp using cellulase mixture produced by recombinant Aspergillus oryzae.
Toshihide YOSHIE1, Shoji SAKAI1, Fumiyoshi OKAZAKI2, Chiaki OGINO1, Tsutomu TANAKA2, Hiromoto
HISADA3, Shin-ya HAGIWARA4, Yoji HATA3, Akihiko KONDO1 (1Dept. Chem. Sci. Eng., Grad. Sch. Eng., Kobe
Univ., 2Org. Adv. Sci. Tech., Kobe Univ., 3Res. Inst., Gekkeikan Sake Co. , 4JPRI)

P-50
ʤǼѺ Phanerochaete chrysosporium _âÖ¿Ţέ˴˻~Ťҧ˞|αβlԒУ~°¤ß¼åÔҧ̅
櫻木 潔 , 堀 千明 , 林 礼子 , 三橋 秀一 , 五十嵐 圭日子 , 鮫島 正浩
1

1

2

2

1

1

（ 東大院農生科、 バイオエタ
1

2

ノール革新技術研究組合）
セルロース系バイオマスの酵素糖化は, アルコールやプラスチックの原料となる単糖を穏和な条件で得るために重要な技術である。酵素糖化を効率的に行うためには前処理が必要であり, その一つにアンモニア処
理がある。これまでの研究によって, アンモニア処理はシラカバやヤナギなどの広葉樹材に対して効果が高いことを見いだした。しかし, バイオマスのどのような変化が酵素糖化効率を上げているかに関しては依然不明な点が多い。そこで本研究では, シラカバの未処理木粉とアンモニア処理木粉を基質として担子菌
Phanerochaete chrysosporiumを培養し, 得られた菌体外液に含まれる主要なタンパク質のセクレトーム解析を行うことで, アンモニア処理が本菌による菌体外酵素の生産に与える影響について調べた。その結果, アン
モニア処理によってエステラーゼやキシラナーゼ, キシログルカナーゼの分泌パターンに変化が起こっていることが明らかになった。このことは, 本菌がアンモニア処理によるヘミセルロース構造の変化に対応して酵素を生産している可能性を示しており, セルロース系バイオマスを糖化する際の酵素選択に重要な情報を与えると考えられた。
Secretome analysis on extracellular proteins of basidiomycete Phanerochaete chrysosporium grown on ammonia
treated wood
Kiyoshi Sakuragi1, Chiaki Hori1, Reiko Hayashi2, Shuichi Mihashi2, Kiyohiko Igarashi1, Masahiro Samejima1.
(1Dept. Biomat. Sci., Univ. of Tokyo., 2RAIB)

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КӔđҾԫӶԒУԄĘǼŪγjq˴˻ў˹Ѻ~НбŤՖƲδ˯ǼնĄƆȸǐ˒Ǽնէȴ̺ͨ մ̂ǫԲäӧαϯյ˴˻ў˹Ѻ~ǪbʤǼѺxYնo~ў˹̬ɞ~ԀZ^ҜѬў˹ѺyϊѬў˹Ѻ|ŤՖhQӪɍնʤǼѺ~ϵԪäȪԪ~ՖоԫİҺ{z~ŤǼНбҧ̅|նÝÐ²åÔ DNA ~ ITS ՑǖԎŦ_Ւс|ŪγhwZQfx΄я Phi29 DNA ÑÝÕÜå±|ՊΤξϋ{ DNA ǣɊնʤǼѺΤξϋËÜÒåγZq ITS Ցǖ~ PCR ǣɊ|Ъƣnն̨ɴԚ~ұˋô~ʤǼѺƥȉlʘ͜ϢϾjqQj^j{_ն˴˻ў˹Ѻ~ ITS ՑǖԎŦ^ɰqŤǼНб̲ϊѬնҜѬў˹Ѻ_ͳǏjwZQòяŕ|˴˻ǪКմ°Þàå®yÌÓ°Þàå®յŤҧlfy_x`ΔxŕӳlQofx
˷ϟϹxնКӔđҾԫӶԒУ|Ϙϑjն˷ԒУԄĘǼ|˴˻ў˹Ѻ~НбŤՖҎtqQ§ÂÔ»å´Íå®|ωԢhwZϵR~˴˻ў˹ʤǼѺ~̬R{ԒУԄĘǼ~ԎŦƚɰjնНб
̲~ĥʒҎtqQo~Ь̈նITS ՑǖԎŦ~Нб̲xϊѬў˹ѺնҜѬў˹Ѻ_ÃÜ´¦зŚ|ͳǏjwZ~|ȘjwնGAPDH ԄĘǼԎŦ~Нб̲xϊѬў˹ѺնҜѬў˹Ѻopũ~¤ßå½ɦʒjնѬɶ|ŤՖyçѧjqQqնҜѬў˹Ѻ 2 u~¤ßå½|Ť^w]նçu˴Қ~̆˴Յ̏{zȨǩ|ն[çˎğȂԌ˻{zȨŚ|ψαl˴˻ў˹ѺxYtqQf~[|նf 3 u~¤ßå½|Ȫlop~˴˻ў˹Ѻ¤ßå½gy|̣ʃΰǢŷȍȘӅ_ξ{twZqQĒë^ն
GAPDH Ї~КӔđҾԫӶԒУԄĘǼ|ǘvbНбŤՖ˴˻ў˹Ѻ~ΤɿѬɶ|ǘvbНбŤՖyçѧlfy_˘^y{tqQ
Phylogenetic classification of wood rotting fungi using enzyme genes related to carbohydrate metabolism
Tomoko Wada, Kiyohiko Igarashi, Masahiro Samejima(Dept. of Biomaterial and Science, Univ. of Tokyo)

P-52 (O-8)լѺ A. oryzae ~Ѻ̔ɦʒıӷǆǼ SclR ~ӽŲψΫ̓γZqѺМҌƣњ~ҧ̅Ʋδձǭ, ԛ ԟŀ 1, ȟȬ͠Ă 1, õȬΉç, ƃ˷žf (̂ǫԲäӧαϯäɼαȾ,1 ԙδβϟ)լѺ A. oryzae ˮɿðđ_Ҡu^tw]m,қˆ~̓~Ŋѫ{ғΫǔƥ˞|ł\q̓~іϵŹΪϋ|Ҏ[fy_ǈՃxYQj^j,§ÂÔҧ̅|ˮɿάԫӶԄĘǼufy_˘^|{,ˮɿάҎ[ƞњɿ_ϥhwZQçˎx,A. oryzae |]ZwѺ̔(sclerotium)~ɦʒњ,ѺМҌƣњ{z_ĞìjwZfy_ˮɿðđ~ψҠǈՃ|jwZçǆyю\QѺ̔yѺМ_ҌƣхӫjwȒՀjqƏѡ~ȟЖǟxY,o~ǩҠˮɿðđ~̫ӵxYǼǃ̓(perithecium)yĜwZQѺ̔Śxʰƣǔ~ξ{Ѻ̓~ѺМ_Ҍƣj,op~̔_ƥçЦјŚ|ǾǏl,̔Ҍƣä͸ˆŤҕЫwˮɿјǼ_ɦʒh~x{Z^y˳ɭhQj^j,fx A. oryzae xѺ̔ɦʒѺМҌƣ|ԫl
ϟϹ_ yzӷxZ{Zfy^,o~ˮɿάњ|ԫlϛҠՊɉ|újZQ˷ϟϹx,̔opлѬ]ӕѬҋŏxƞҢƂlq~ҌƣԄĘǼ sC ԄĘǼɘ|ʫőjq
̓|,Ý­â/Ü¬ÞҞ͍ɿ(ΔpyrG)q»¿âҞ͍ɿ(ΔadeB)Đîjq̓ĥҚjqQojw,f
~̓ȔǬǗǑxȘȲǗ՜,jbͭĢǗǑxͳƣǗ՜hnfyx,ѺМҌƣњҺqQo~Ь̈,]ăZ~̑՜Ҟ͍ɿ_ϓҙhˬȠǗǑxαіƞњ{̓ƚɰj,o~ѺМҋŏՕɴԦxҥȖly̔|лѬäӕѬòˎ~ҋŏ_yqQĒëξ{̓~ғΫǔ_ƥç~̓|Ϋqfy^,A.
oryzae |]dѺМҌƣњҧ̅l¬®ºÔϢϾlfy|ʒŶjqQΫǏ,Ѻ̔ɦʒıӷǆǼ SclR ӽŲψΫhnfy|,ѺМҌƣњ~Ź̈̥ҫjwZQ
Analysis of hyphal fusion ability by using the strains overexpressing SclR, a promoting factor for sclerotia
formation in Aspergillus oryzae
Ryuta WADA, 1Feng Jie JIN, 1Yasuji KOYAMA, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo, 1Noda Ins. Sci. Res.)

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Aspergillus nidulans ~ NmrA y AreB |Õ¾­âѐɿƂ̴̫~ҧ˘Ė҇ѲԗǼն̜ȣĲč, ɻ͋ŌĈ, եӂϒ̲ մЈ͞ǫȀäαƱΰǢյ
A. nidulans ~ϽУđҾНԄĘǼ~ψΫūɱlӞŞǆǼxY NmrA PNAD(P)H |͈ NAD(P)+|եZҤƲɿϥlfy^αĢŚ~ NAD(P)+/NAD(P)H ͈ʎϛl°âªåyjw̴њlfy_ĀʊhQfxʓRPNmrA _ glucose-6-phosphate ѝ͋УԒУP6-phosphogluconate ѝ͋УԒУ]
glycerol ѝ͋УԒУ~ԄĘǼ~ψΫ̺|ūɱlfyƕ A. nidulans ~αі~Õ¾­â(MD)|ȘlѐɿƂ|ȑîjwZfyϥjqQ˷ϟϹxPNmrA Ьƣ´âÅ¤Ӕyjwϛ AreA yƥ̬|
GATA ½Õâʧu AreB _˷Ѻ~ MD ѐɿ|ԫîjwZfyҠţjqQBimolecular Fluorescence
Complementation ͜] NmrA y AreB ~Ъʴ\´âÅ¤ӔγZq GST pull-down ͜|PNmrA y
AreB _ϓă|ЬƣlfyϥjqQqPòя~ in vitro x~Ьƣ NADP+|ıӷhPNADPH |ʜūhqQqP¤àÒ¶âŐπ͒԰͜|PëҬ~ԄĘǼËàÖå´å| AreB _Ьƣlfy
˘^yjqQNmrA  MD |ԓƂϋ|αk NADP+ʎϛjPAreB yϓăĥγlfy|twëҬ~ԄĘǼ~ψΫͥɿƂj NADPH ~αβıӷhn̴њʧuyю\qQ

The Aspergillus nidulans NmrA and AreB are involved in menadione stress tolerance.
Eriko Ito, Shunnsuke Masuo, Motoyuki Shimizu, Naoki Takaya(Graduate Sch. of Life and Environmental Sci., Univ. of Tsukuba)

P-54
Aspergillus oryzae ~®¼ß®˿Ĕìx~αіҺЎ|ԫӞŞǆǼ~ҧ̅կδ˘Řնեӂϒ̲մЈ͞ǫԲäαƱΰǢյDNA Ò¤àßҧ̅yÝÞ´Ô PCR ҧ̅γZwնĞԓУ˿ĔìxΤξϋ|ψΫԚ_ǣŷlӞŞǆǼԄĘǼ AO090005000179 (hypB)ҠţjqQf~ԄĘǼ~ԄĘǼϠǥ̓մΔHYPնԙδβ̧ϯȀϟϹʖյyԙἀնЪʒ̬R|ʾǧjqǋĢǗǑëxǗ՜jq_նӳ͊˿Ĕ|ԫİ{bo~¨à¿å~ɦʏyαіӴɗ^{^tqQ̷|նΔHYP ͭĢǗ՜jqyf ĞΓУ˿Ĕìx~αі_ԙἀѫǴrtqQqնò̓~ŤαǼ~ψѰΪy¥Þ¨å®~ͬӐԚ͈ӡjwyնΓУͽ~Ύɗ|ԫmΔHYP ԙἀɉ|եZψѰΪyͬӐԚϥjqQojwնf~ɀĞΓУͽΎɗìxˬՕҀy{tqQçˎնαі_ԌŤϋ|ʜūhĞ pHնե pH {z~®¼ß®˿ĔìxնΔHYP ԙἀѫǴ|αіjqQqնf~y`նΔHYP ~αіʞϖѺŰ|ȘjwӘʎƛɿxYtqQĒë~Ь̈^նhypB ĞΓУͽնĞ pHնե pH {z~αіūԱl®¼ß®˿ĔìxնA. oryzae ~̑՜ǣ́ʜūhn̴њʧufy_ϥhqQh|նhypB ~եψΫ̓մHYPյĥҚjնϠǥ̓yƥ̬~ҧ̅ҎtqQo~Ь̈նHYP x brlA ~ψΫ~ҵȞն]ŤαǼ~ɦʒ˞˳~˔˳ƂyϘαˆ~ǣŷ_qQqնHYP ~¨à¿å|ˑǾ~ŤαǼ̌~ŎЀ^qѺМ_ěԧjնˍq{ŤαǼ̌ɦʒjwZΤξϋ{̫ӵմĂ̷ŤαǼ̌յ_ϲ|qQĒë~Ь̈^նhypB ŤαǼ~ɦʒͥɿƂhn̴њuƞњɿ_ϥhqQTranscription factor regulating growth and development of Aspergillus oryzae under stress conditions
Akinori KurodaնNaoki Takaya(Graduate School of Life and Environmental SciencesնUniv. of Tsukuba)

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P-55
Îß¿ÞÜ¥Ü®ŕαѺ Epichloë festucae ~ ProA _ЬƣlËàÖå´åԎŦ~ƥȉδôʍǼ 1նSanjay Saikia2նGemma Cartwright2նЂ˷ǫƬ 1նŷ҇ԿǦ 3ն̤̎ȡɻ 1նBarry Scott2մ1 ƦǫԲäαӧն2Massey Univ.ն3 ƦǕǫäӧյEpichloë â½É¼նȐöÎß¿ÞÜ¥Ü®~ЦјԪԺěԧjwŕαlQŎ|նE.
festucae |]ZwնǼ~[Ѻ~ˮɿά|ԫîl Zn(II)2Cys6 ´Ë~ӞŞūɱǆǼ ProA/NosA yϓƥɿϥl EfProA ƥȉjqQEfPROA Ϡǥ̓xնȐöŚx~ѺМαі_ԙἀ|͈Ҁjbıӷhնʰϵ̤Σ|αіԮȍ̊̾ɟ`ӗfjqQh|նEfPROA Ϡǥ̓|]ZwնAspergillus nidulans ~ˮɿά|ɹՐ{ esdC ~ϓƥԄĘǼմEfESDCյ~ӞŞßÍÞ_ՕҀ|ĞìjwZfyҠţjqQofxն§Þ¬É¼¸°͜γZwնProA ~ EfESDC ËàÖå´åԎŦ~Ьƣͥɿ|uZwҺ̐jqyfն
ProA _ EfESDC ËàÖå´å~ GCGCCG ԎŦ|Ьƣlfy_˘^y{tqQEfESDC ӪĿ~§ÂÔԎŦҧ̅jqyfնEfESDC ëͦ~Ӱԥ|¨å½hԄĘǼմEF320յ_ҠţhնEfPROA Ϡǥ̓xն
EfESDC yƥ̬| EF320 ~ӞŞßÍÞ_ՕҀ|ĞìjwZfy_ϢҴhqQEfESDC y EF320 ~ 2.4 kb~ԄĘǼԪՑǖմËàÖå´åՑǖյ|նProA ЬƣԎŦմGCGCCGյ_ 1 ^ʖ~ǾǏlfy^ն
ProA f~ԎŦ|Ьƣlfy|նEfESDC y EF320 ~ψΫƥҺϋ|ūɱlfy_ϥƷhqQ
Identification of the promoter sequence for the ProA binding in Epichloë festucae, a mutualistic symbiont of
perennial ryegrass.
Aiko Tanaka1, Sanjay Saikia2նGemma Cartwright2նDaigo Takemoto1նMasashi Kato3նTakashi Tsuge1նBarry
Scott2 (1Grad. Sch. Bioagric. Sci., Nagoya Univ.ն2IMBS, Massey Univ.ն3Dept. of Agr., Meijo Univ.)

P-56
Aspergillus nidulans AmyR ~ҵȞΣӔĬǾϋ{Ťҧ|ԫlҧ̅
ǫȶԴǯ,

̢˷ыǭ,

ԛõĉǼPŷ҇ԿǦPȟ̇ƶǮ մƦǫԲäαƱӧȀյAspergillus Ȫ|ˢӼϋ|ǾǏlӞŞǆǼ AmyR »âËâŤҧԒУԄĘǼщ~ӞŞ̺|ūɱjwZ
Qfx|, AmyR αέϋҵȞΣӔxY isomaltose (IM)|ɼЉjw̔ϱҎjPӞŞͥɿƂlfy_˘^|hqQq, IM ĬǾϋ|ÝâԓƂƛdfyPo~ɮŤҧhfyϥhwZQ
fҵȞ˿Ĕ^ՊҵȞ˿Ĕ~ϱҎ~ԻPӞŞ~ļ̹_ AmyR ~Ťҧ|ҎfyϥƷjwZQf~ IM ĬǾϋŤҧ~Õ ¿¯Ô˘^|lqP˷ǝƭxƢϵ AmyR ҵȞĢγZwo~Ȅȉɿҧ̅jqQ GFP-AmyR ԙαǔ AmyR yƥЇ~ɿӔʧsPIM ĬǾϋ|̔ϱҎäŤҧhQҵȞ¬¥¾Þƛȏ
½Õâ|Δǧξʧu GFP-AmyRH478LP̔ϱҎ¬¥¾Þ|ǧξʧu GFP-AmyRpmw ̔ϱҎj{ZQf~ҵȞĢ GFP-AmyR ȄȉxYPŮяx IM ĬǾϋ{ŤҧҥȖhmPɮяxŤҧҀjbӠ͸hwZqQ̔ȥǏ_Ťҧɟ`ӗflƞњɿ̥үlqPSV40 ὲ~ NLS Ҍƣjq NLSGFP-AmyR ĥҚjqQ˷ҵȞĢĀʊӳɉ|̔|ȥǏjPIM ՊǾǏìxíȄȉƂjwZqQqP
IM ǾǏìxh|Ťҧ_ıӷh GFP-AmyR yƥϳɗ~ƈ͸˳ϥjqQNLS-GFP-AmyRpmw PΫǏPέεí˘xY_PIM ĬǾϋ|̔ϱҎlQ˷ҵȞĢ GFP-AmyR y ƥ̬| IM ĬǾϋ|ŤҧhqQĒë^PIM ĬǾϋ{ AmyR ~Ťҧ IM ĬǾϋ{̫ӵǧƂy̔ȥǏyZ[Ău~Ҟǆ|ɟ`ӗfhfy_˘^y{tqQ˷ϟϹ~çԌPαϟ°â´å~ÂÍå¬ÚâŴţā̧|ʽʶƛdwҎq~xYQAnalysis on inducer-dependent degradation of AmyR in Aspergillus nidulans
Akio Osaki, Shota Morimoto, Kyoko Kanamaru, Masashi Kato, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ.)

66

P-57
Aspergillus aculeatus ace1 ̶ʷ̓|]dКӔŷ͋ŤҧԒУ~αβyǡ®¼ß®ɼЉ~ҧ̅ȬղʍɡնȟҝȆƲնӂĵ͘նΓӂՏçնȺƜůơ մǫԬɖϾǫäαΰϯյМΥѺ Aspergillus aculeatus КƂŵ|Ŋq°ÞÜå±αβlQofxʓR˷Ѻ~°ÞÜå±ԄĘǼψΫʜū|ԫǆǼϠǥjնfԒУŹΪϋ|αβl̓~іϵϑʨjwZQ
Trichoderma reesei AceI °ÞÜå±¢¬Ü¾å±ԄĘǼ~ψΫʜūlӞŞʜūǆǼxYQçˎնÞ²à¥~ Aspergillus nidulans StzA ǡ®¼ß®ɼЉ|ԫîjw]նAceI/StzA Þ²à¥Ԫx̴њ~ՖĜɿҠmնA. aculeatus Ace1 ~̴њՖʱlfyx`{ZQofxն˷ϟϹx Aspergillus
aculeatus ace1 ̶ʷ̓ĥҚjնA. aculeatus Ace1 ~̴њҧ̅jq~xǝƭlQ
A. aculeatus ace1 ̶ʷ̓|]d endoglucanase, endoxylanase ͥɿն¨â¼àåÞ̓yƥϳɗxYtq_նβ-glucosidase(BGL)ͥɿն¨â¼àåÞ̓~П 2 ĸ|ǣŷjqQ qնace1 ԄĘǼ̶ʷ̓ 100, 300, 500 mM NaCl ƪ YPD ǗǑëxαіhnyն¨â¼àåÞ̓y͈w 300, 500 mM NaCl ʹŷǗǑëx~ѺМěԧyјǼɦʒ_ҀjbĞìjqfy^նA. aculeatus Ace1ǡ®¼ß®ɼЉ|ԫîjwZfy_ϥƷhqQ
 Čɮն A. aculeatus BGL ~öҞ{ԒУxY BGL1 ԄĘǼјǼɦʒ|ԫîlԄĘǼ~ψΫԚȉԚlfyxնAce1 ~̴њҧ̅lҪθxYQ
Production of biomass degrading enzymes and salt stress response in Aspergillus aculeatus ace1 deficient mutant
Manami Yamazaki, Hirokazu Konishi, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi( Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.)

P-58
Aspergillus aculeatus Zn(II)2Cys6 ǔӞŞǆǼ CGAF °Þàå®ɿǘӔ|ɼЉjw°
ÞÜå±äÌÓ°ÞÜå±ԄĘǼψΫͥɿƂlǍ̻вшնӂĵ͘նΓӂՏçնȺƜůơ մԬɖǫԲäαΰϯյ
Aspergillus aculeatus ~ cellobiohydrolase I ԄĘǼ (cbhI) ƕ carboxymethyl cellulase 2 ԄĘǼ (cmc2) ψΫӞŞǆǼ XlnR |ĬǾj{ZЫӚčjw°Þàå®|ҵȞhQf~˵ƥȉ~°Þàå®¬¥¾
ÞĘӿЫӚ|ԫîlǆǼ®¤Ýå¿â¥jն Zn(II)2Cys6 ǔ DNA Ьƣ½ÕâʧuǆǼ cellulase
genes-activting factor (cgaF)ƚɰjqQCGAF ~̴њҧ̅Ҏ[qնm cgaF Ϡǥ̓ĥҚjնȉԚ
PCR |°ÞÜå±ԄĘǼ~ӞŞҧ̅ҎtqQЬ̈նcbhI y cmc2 ~°àÇå®yÇ°ÞҵȞ˿Ĕì|]dψΫ_¨â¼àåÞ̓|͈wĞìjqQXlnR ĬǾϋ|ψΫ_ūɱh cmc1 ƕ FIbxylanase ԄĘǼ (xynIb) ~ψΫÇ°ÞҵȞ˿Ĕìxƥ̬|͸Ƞjq_ն¢¬àå®ҵȞ˿Ĕìxɀ_Ҵ{^tqQqնtranscription elongation factor 1α ԄĘǼ (tef1) ËàÖå´åūɱìx cgaF ̫ʒϋ|եψΫjq̓xն°ÞÜå±ä¢¬Ü¾å±ͥɿ_Ǘ՜ 10 ˒ϑ|¨â¼àåÞ̓~ 2 ĸƕ 5 ĸë˖jqQf~Ь̈^ CGAF °Þàå®¬¥¾Þ|ɼЉjq°ÞÜå±äÌÓ°ÞÜå±ԄĘǼ~ӞŞͥɿƂǆǼyjw̴њlfy_ϥhqQ

Zn(II)2Cys6 type transcription factor CGAF activates expression of the cellulase and hemicellulase genes in
response to cellulosic carbon sources in Aspergillus aculeatus
Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi

67(Grad. Sch. Life Env. Sci., Osaka Pref. Univ.)

P-59
МΥѺ|]d°ÞÜå±αβūɱ̴̫ՇȬ˵̀նԛõĉǼնȟ̇ƶǮ մƦǫԲαƱӧäαΣ̴̫յ
VϑϋW Aspergillus oryzae |]ZwƥȉhqӞŞǆǼ ManR PÒâÂÇå®|ɼЉjwÒâ¾¾
å±ä°ÞÜå±~ψΫҵȞlQA. oryzae °ÞÜå±αβɿ_ĞbϟϹ_ǈՃxYqP˷ϟϹ
x A. nidulans |]d ManR ϓƥǆǼ~ҧ̅ҎtqQVˎ͜äЬ̈W ManR ϓƥǆǼ~Ϡǥ̓ĥʒjP°ÞÜå±]Òâ¾¾å±~Ëßå¼¸°|ījqyfP°ÞÜå±αβɿǰq_PÒâ¾¾å±αβɿ|ɪՍҠ{^tqQqP

CMC ] konjac glucomannan ƋçΓУͽyjwͭĢǗ՜jPo~Ǘ՜ë͵«Ö¥ÜÉå|ҧ̅jqyfPöҞâ½¥Þ¾å±xY EglAPEglB ~αβ_yzҠmPçˎP4-5 ϵҠÒâ¾¾å±~[s 2 ϵ~αβ_Ҁjb͸ȠjqQf~ 2 ϵ~Òâ¾¾å± EglAPEglB yƥ̬|ÒâÂÇå®x{bP°àÇå®|ҵȞhqQɯtwPManR ϓƥǆǼ°àÇå®|ɼЉ
jw°ÞÜå±yçԌ~Òâ¾¾å±~αβūɱlǆǼxYP̴њϋ| ManR yҩ[j
CelR xYyю\QqPÒâÂÇå®|ɼЉjwÒâ¾¾å±ψΫūɱlӞŞǆǼũ|ǾǏlfyϥƷhqQ
˷ϟϹ~çԌPαϟ°â´å~ÂÍå¬ÚâŴţā̧|ʽʶƛdwҎq~xYQ
Regulation of cellulase synthesis in filamentous fungi
Miki Aoyama, Kyoko Kanamaru, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ. )P-60 (O-10)
լѺӞŞǆǼXlnR~ҵȞΣӔĬǾϋÝâԓƂyo~αέȀϋʌъϝȺƮɌ, ԙƜϨơ, ԛõĉǼ, ŷ҇ԿǦպ, ȟ̇ƶǮմƦǫԲäαƱӧ, պƦǕǫäӧյ
VϑϋWլѺ Aspergillus oryzae ӞŞǆǼ XlnR , ҵȞΣӔ¢¬àå®|ɼЉjw¢¬Ü¾å±,°ÞÜå±ԄĘǼщ~ӞŞͥɿƂlQfx|, XlnR _ҵȞΣӔĬǾϋ|ÝâԓƂĵ՛hfy˘^|jqQ˷ϟϹx,XlnR ~ҵȞΣӔĬǾϋÝâԓƂ~ҳЦ phosphate affinity SDS-PAGE γZwҧ̅jնq,ÝâԓƂ~αέȀϋʌъ~ҧ˘ϑʨjwնÝâԓƂ_ XlnR ~Ťҧ DNA Ьƣ|î\ɪՍҧ̅jqQVˎ͜yЬ̈Wc-myc Ҍƣ XlnR ψΫlլѺ~Цјʣţͭ phosphate affinity SDS-PAGE |īj,®
´âÊà¸ºâ¥ҧ̅| c-myc::XlnR ̥ţjqQo~Ь̈,ՊҵȞ˿Ĕì|]Zw XlnR ÝâԓƂßÍÞ~ξ{ 3-4 ϵ~ŤǼϵyjwǾǏjնҵȞ˿Ĕì|]ZwÝâԓƂßÍÞ~ë˖jq 2 ϵ~ŤǼϵ|ƙ˾jqQf~Đŷϋ{ÝâԓƂƞӰϋx,¢¬àå®~Գƒ|ՊҵȞ˞~ÝâԓƂßÍÞxĞìjqQÝâԓƂßÍÞ~ԀZ XlnR ~Ȅȉɿ|ɪՍî\{^tqQChIP Assay ] XynF1 ~ËàÖå´åՑǖËàåÊyjq EMSA |,Цјʣţͭô~ XlnR ¢¬àå®ՊĬǾϋ| DNA Ьƣlfy_ϥhqQĒë^,XlnR ɉ| DNA ë|ǾǏj,¢¬àå®ĬǾϋ{ƞӰϋÝâԓƂĵ՛|ͥɿƂh,ìͦԄĘǼ~ӞŞҵȞlyю\Q˷ϟϹ~çԌPαϟ°â´å~ÂÍå¬ÚâŴţā̧|ʽʶƛdwҎq~xYQ
Inducer-dependent phosphorylation of the transcriptional activator XlnR and its physiological role in Aspergillus
oryzaeShuhei Ishikawa, Yuji Noguchi, Kyoko Kanamaru, Masashi Katoպ, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ., պSch. Agric. Sci., Meijo Univ.)68

P-61 (O-11)
Trichoderma reesei ~°ÞÜå±αβ|ԫîl¬¥¾ÞĘӿԫӶ´âÅ¤Ӕ~ʯФ˒ìϭҎնƝȺԶО, ͰӂѲƿ, ɻδ͢čնȟЄƐ ͷ մԧȮʚϯǫäαΣյ МΥѺ Trichoderma reesei նЦјǩ°Þàå®o~ҵȞĢ|ɼЉjw°ÞÜå±ҵȞϋ|αβlQj^jնҵȞ~ŧ͂Էx°Þàå®íͿɿxϒʰЦјѡӳӽx`{Zfy^նz~[|Цјǩ°Þàå®ҴҿjwZ~^í˘xYQqնfx~ϟϹ^ŧψ~°ÞÜå±ҵȞαβ̫ʒϋ|ψΫjwZ°ÞÜå±|twŤҧhqɴԚ~°Þàå®ŤҧβΣ_ɟ`ԛy{ɟ`ӗfhyZ[Ö»Þ_ʳƹhwZ_նo~ҳЦ˘^|hwZ{ZQofx˷ϟϹxն°ÞÜå±ҵȞΣӔ~ƚөЫӚ]ǘӔҴҿ|ԫlϛҠɰfyϑϋyjwնҵȞψΫ|ԫl¼Üâ®Ñå´å~ƥȉұqQT.reesei §ÂÔ»å´Íå®Ś|ǾǏlŒ 9129 ԄĘǼ^КӔӥӯ|ԫîlyʱȉh¼Üâ®Ñå´åԄĘǼʣţjնÒ¤àßγZwϵR~ΓУͽ|ȘlψΫɼЉҧ̅ҎtqQo~Ь̈ն°ÞÜå±ԄĘǼyƥҺjwψΫjwZտķ~¼Üâ®Ñå´åҠţjqQf~¼Üâ®Ñå´å|uZwն°ÞÜå±ҵȞ~ԫî˘^|lq|ƢԄĘǼ~Ϡǥ̓̫АjնÇ°ÞǾǏì|]d°ÞÜå±αβɿ~ҧ̅ҎtqQo~Ь̈նYԄĘǼ~Ϡǥ|ĚZ°ÞÜå±αβ
њ~ҀjZĞì_ҥȖhqQΫǏնf~Ϡǥ̓γZw°àÝ©К~ƚө]°ÞÜå±ԄĘǼψΫ|uZw~ҧ̅ҎtwZQ
Functional analysis of genes encoding a putative cello-oligosaccharide transporter in the filamentous fungus
Trichoderma reesei.
Hideyuki Kusaka, Takanori Furukawa, Eiji Fukaya, Yousuke Shida, Wataru Ogasawara(Deot. Bioeng., Nagaoka Univ. of Tech)

P-62
МΥѺ Trichoderma reesei |]dˍҡҵȞψΫ̴̫~ʯФĠ҇˛ѻնՄϖɠնȟЄƐͷ մԧȮʚϯǫäαΣյ
Trichoderma reesei |]Zwնfx|°Þàå®C¢¬ÜâНҵȞΣӔ|°ÞÜå±CÌÓ°ÞÜ
å±ҵȞψΫ̴̫_ώ|ϟϹhw]նf~ԄĘǼ~ӞŞūɱ|]Zwôɸϋ{ɫųʤtwZӞŞǆǼ_ XYRI xYfy_lx|ǝƭhwZQçˎxնT. reesei _ѥΘιxαіlΰǢô|̬R{̑՜ͽ_ǾǏjPo|ɼЉjwҵȞh˵ϛ~´âÅ¤ӔψΫ̴̫_ǾǏlfy_ю\Q˷ϟϹxն̬R{̤ΣЦјǤ̫ʒʒŤ~ψΫɼЉ˘^|lfy|նfxçԌ~ҵȞΣӔ|ԱwZqψΫ̴̫|ˍq{ϛҠŷ\fy|PѥΘι|]d T. reesei ~ҵȞψΫ̴̫ҧ˘lfyϑϋyjqQ
̬R{̤ΣЦјǤ̫ʒʒŤΓУͽ|Ǘ՜ҎZնo~Ǘ՜ͭ SDS-PAGE jqЬ̈, ΓУͽΤξϋ{ψΫʩŻϥlɐu^~´âÅ¤Ӕ˘^|jqQo~[sն¡Ü¤¼å®Ї|ɤZɼЉɿϥjqÄâ½ӔԚŤ̅xҧ̅jqЬ̈ն®ÅÜ£âԓËàºå±_ƥȉhqQf~ԄĘǼ~ψΫ|ˑϛ~°Þ
Üå±ԄĘǼҺЎǆǼ_ԫîjwZ~^˘^|lq|նXYR1, ACEI, ACEII, CreI Ϡǥ̓γZwҧ̅ҎtqQo~Ь̈P®ÅÜ£âԓËàºå±_fˑϛ~ӞŞūɱǆǼ~ūɱì|{Zfy_ϥƷh, ˵ϛ~ҵȞψΫ̴̫~ʽԎì|Yfy_ю\qQΫǏ, ˍҡӞŞҺЎǆǼ~Τȉ|ƧdwԄĘǼëͦՑǖ~ҧ̅Ҏ[yy|PÒ¤àßγZqψΫԄĘǼ~ичϋҧ̅ҎtwZQq, ˵ƥȉ´âÅ¤Ӕ|ȘjwӔԚŤ̅ҎZ, ˍҡҵȞψΫ̴̫~ψҠ|ƧdwǪˎՋ^ϟϹӷw
ZQ
Search for novel transcriptional mechanism in Trichoderma reesei
Haruna Sato, Masahiro Yuki, Wataru Ogasawara(Dept. of Bioeng., Nagaoka Univ. of Tech.)

69

P-63
Trichoderma reesei ~͈ӡ§ÂÔҧ̅|ǘvbˍҡӞŞǆǼ ClbR ~̴њҧ̅ˍδшӏǼ 1,2, ȬƜ՟ф 1, ɻδ͢č 1, ̢ç̲ 3, ɌȺϭ̲ 4, øƐƶ 3, ̢Ⱥɚ 1, ȟЄƐͷ 1մ1 ԧȮʚϯǫäαΣ, 2 JST, 3 üǫäαΣӒͽ, 4 ^mh DNA ϟϹʖյVϑϋWTrichoderma reesei ~°ÞÜå±եαβǧξ̓ PC-3-7 yo~Ҥ̓xY KDG-12 y~͈ӡ§ÂÔҧ̅նORF Ś|çǡǘǪǔմSNPsյ_αkqԄĘǼ_ 9 uϢҴhwZQAvicel Ƹç~ΓУͽyjwòǧξ̓Ǘ՜jqyfն°ÞÜå±αβɿ|ǫ`{ɀξ_Ҵ{^tq~~նΓУͽ 2 К
xY°àÇå®yjqy`|նPC-3-7 ̓x°ÞÜå±αβњ_՗ӛϋ|Ƨëlfy_˘^y{tqQʓRf~[{ǧξ̓Ԫ|]ZwƥȉhwZ 9 u~ SNPs ~Zm^|ӗǆl~yʊȉjնŗR~ԄĘǼϠǥ̓~ҧ̅ӷwZQ
SNP _αkwZ 9 ϵ~ԄĘǼ~ 1 u|ʱȉ~ Zn(II)2Cys6 ǔӞŞǆǼ_ƪw]նʱȉ DNA ЬƣԌĝӪĿ~ÓÂԓ_цʴhwZfy_˘^y{tqQɥҲӞŞǆǼմClbR:Cellobiose responsing
Regulatorյ~ÏÖà¥ Blast x̥ФjqyfնϵR~ϖѺΤξϋӞŞҺЎǆǼ~{^xնAoAmyR |ˬեZϓƥɿϥjqQAmyR նAspergillus Ȫ|]d 2 КxY²ÒÞ¼å®|ÓÜå±ԄĘǼщ~ӞŞҺЎǆǼxYQo~qնPC-3-7 ̓|]ZwɤƂhq°àÇå®|°ÞÜå±~αβɿ| ClbR _ԫîjwZƞњɿ_ю\qQ˷ϟϹx ClbR ҧ̅~´å§¸¼yjն°ÞÜå±~ҵȞψΫ̴̫|ԫlˍq{ϛҠɰfyϑϋyjwZQ
VЬ̈WPC-3-7 ̓|uZwնclbR ~ SNP ԙἀմQM6aյ~ԎŦ|ɲɈjqǧξ̓մɲɈ̓յն]
 ORF ԌĝϠǥjqϠǥ̓ĥҚjqQKDG-12նPC-3-7նɲɈ̓]Ϡǥ̓|uZw°àÇå®ҵȞǗ՜˿Ĕ|]d°ÞÜå±ͥɿնΓУͽ~ŤҧäƚөʩŻն]°ÞÜå±ԄĘǼ~ψΫʩŻҧ̅jqyfնɲɈ̓նҤ̓~ KDG-12 yƥЇ~ĺçӶ~ҧ̅xϥjqQ˪|նclbR ~ SNPմPC-37յ̓]Ϡǥ̓xնɲɈ̓y͈ӡjw°ÞÜå±ͥɿ~ǫɊ{ƧëնΓУͽ~ŤҧäƚөӹɝնΤȉ~=-¥Þ¨¬µå±ԄĘǼ]ӞŞǆǼ~ҺЎմψΫʜūäӹɝյ_ϥƷhqQ
Identification of new Zn(II)2Cys6 type transcription factor involved in cellobiose hydrolysis in Trichoderma
reesei
Mikiko Nitta1,2, Kaori Yamaguchi1, Yosuke Shida1, Kazuki Mori3, Hideki Hirakawa4, Satoru Kuhara3, Yashushi
Morikawa1, Wataru Ogasawara1 (1 Nagaoka Univ. of Tech., 2 JST, 3 Kyushu Univ., 4 Kazusa DNA Inst.)

P-64լѺËàºå± AOEXE103 ~Þ´¾ºÊ®ËÜ¬â¥øĳȴʄ̢δȕĊŮδͨȮ˷йǼȬɦ͢ɌЂŚӾԾմ̂ĉӧȾǫԲäӧäɼαƂյ
VϑϋWլѺ Aspergillus oryzae ~§ÂÔҧ̅|նA.oryzae ~ 12 ϵ~°Ýâ´Ë ÞÐ¢¬ÎË¶µå±(CPase)ԄĘǼ~ǾǏ_˘^y{tqQAOEXE103 o~ 12 ϵ~ CPase ~Ś~ 1 uxYնf~ԒУ¨å½lԄĘǼնŒԧ 1775 bp x 3 u~â¼àâƪyʱȉhwZQ˷ϟϹxնo~mRNA ~ԎŦҺqyfնçԌ~â¼àâ_Գƒh{ZÞ´¾ºÊ®ËÜ¬â¥~ƞњɿ_ϥƷhq~xo~ҳЦ|uZw̥ҫjqQVˎ͜äЬ̈WA.oryzae RIB40 ̓ N ͽ~ξ{ Czapeck-Dox ͭĢǗǑxǗ՜jնof^Ы˞ϋ| gDNA Œbƪ{Z mRNA ЙҚjwӰӞŞƘɼҎ[fyx̬R{Ǘ՜˿Ĕ~ cDNA ɰqQo
~ cDNA Ԡǔyjw AOEXE103 ԄĘǼΤξϋËÜÒåγZq PCR ҎZնǫѠѺȐöyjwAOEXE103 ~ cDNA ÜÊÜÝĥҚjqQɰqÜÊÜÝ^ËÜ®Ó½ʣţնâ¼àâô|
Τξϋ|ǾǏlūԱԒУª¼γZqūԱԒУŢέy PCR | AOEXE103 cDNA ~ 3 u~â¼
àâ~ˮΖҧ̅jqQo~Ь̈նmRNA ~ô|ն3 u~â¼àâ_Œw®ËÜ®hq~ǾǏ
jwZq_նçԌ~â¼àâ_Գƒh{ZÞ´¾ºÊ®ËÜ¬â¥|βΣǾǏlfy_
˘^|{tqQh|նAOEXE103 ~Þ´¾ºÊ®ËÜ¬â¥նǗ՜˞ԪĬǾϋնǗǑĬǾϋ|o~Å´åâ_ǧƂlfy_˘^y{tqQ{]ն˷ϟϹαϟ°â´åǘϤʱӷā̧~çΰyjwҎq~xYQ
Alternative splicing of protease gene AOEXE103 in Aspergillus oryzae.
Megumi Kuboshima, Hiroto Morita, Hiroshi Maeda, Ayako Okamoto, Youhei Yamagata, Michio Takeuchi(Tokyo Univ. of Agriculture and Technology)

70

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լѺ°Ýâ´ËÞÐ¢¬ÎË¶µå±ԄĘǼщ~ичϋψΫҧ̅ԯĳ˛ѭն̢δȕĊPȮ˷йǼPŮδͨPȬɦ͢ɌPЂŚӾԾմ̂ӧȾǫԲäɼαƂյ
VϑϋWլѺ A.oryzae ~§ÂÔҧ̅~Ь̈նǡǘԎŦ^ʱȉhÓÂԓԎŦ^նլѺ~§ÂÔ|°Ýâ´ËÞÐ¢¬ÎË¶µå±~ͥɿôɸÖ¶åÉˮlyʱȉhԄĘǼ_ 12 ϵՖǾǏlfy_˘^y{tqQfx|f~ԄĘǼ|¨å½h´âÅ¤Ӕ~ÓÂԓԎŦ|ǘvbНб̲_ĥʒhնͭјȥǏǔն©Þ­ĢȥǏǔնѺĢǩŤ͛ǔ 2 u~Ҫ 4 u~¤Ü®´å|ŤՖhfy_ϥƷhqQj^jնf 12 ϵՖ~ԄĘǼ~ψΫ˞˳նψΫ˿Ĕ{zfx|ҧ˘hwZ{
ZQofxն˷ϟϹx 12 ϵՖ~ԄĘǼ~ψΫ˞˳նψΫ˿Ĕ{z~ҧ˘ϑϋyjqQVˎ͜äЬ̈WA.oryzae RIB40 ̓ͭĢqɌ̄~ Czapeck-Dox ǗǑxǗ՜jնЫ˞ϋ|ѺĢǅƙ
jw mRNA ЙҚɮնcDNA ƣʒjqQɰq cDNA ºâËßå¼|նƢԒУ|Τξϋ{ËÜÒåγZwƈȉԚϋ PCR ҎZնf 12 ϵՖ~ԄĘǼ~ӞŞÅ´åâҧ̅jqQo~Ь̈ն11 ϵՖ~ԄĘǼ mRNA yjwψΫjwZfy_ϢҴhqQj^jնo~ψΫÅ´åâǗ՜˿Ĕ|ξ{twZqQլѺ|Τˮ~ԄĘǼ´âÅ¤Ӕ N ͽyjqǗǑxψΫԚ_ǣǫlŅƧ_ҴqQ
qնČǅ~˿ĔxψΫ_Ҡ{ZԄĘǼǾǏjwZqQ ˷ϟϹαϟ°â´åǘϏϟϹʱӷā̧~çΰyjwҎq~xYQ
Semiquantitative transcriptional analysis of serine-type carboxypeptidase encoding genes in Aspergillus oryzae
Haruka Abo, Hiroto Morita, Ayako Okamoto, Hiroshi Maeda, Yohei Yamagata, Michio Takeuchi(Tokyo Univ. of Agriculture and Technology)P-66҆ Ű ʭ ţ ABC ¼ Ü â ® Ñ å ´ å  ū ɱ l  Ӟ Ş ǆ Ǽ AtrR y ϓ ă ĥ γ l  ǆ Ǽ ~ ʯ ФǫǞ̼նδôίɁնˍӂȡɠնĄƯžýմ̂ƃǫԲӧäαΣβ̧Ŵʒյ լѺ~ӞŞǆǼ AtrR ն³åÞН҆Űʭţlқˆ~ ABC ¼Üâ®Ñå´åԄĘǼ~ψΫūɱjwZշţѰԒ͆xնƥ̬~҆Űʭţ ABC ¼Üâ®Ñå´åԄĘǼ~ψΫūɱ|ԫӞŞǆǼ
PDR1/PDR3 ~̴њψΫ|]ZwնÕ»å´åͥɿƂҙŸǆǼ GAL11/MED15 ªÊÙ¿¸¼_ն̨wԘҞxΤξϋ{ɫų̈qjwZfy_˘^|hwZշj^jնլѺ§ÂÔë| GAL11 yϓƥɿ~եZ´âÅ¤Ӕ_Ҡţhw]mնլѺxţѰԒ͆yƥk[{ӞŞūɱ̴̫_ǾǏj{Zƞњɿ_Yշqնfx|МΥѺ~ӞŞÕ»å´å|ԫӶjqϛҠyz{Zշofxն˷ϟϹxնլѺ|]Zw ABC ¼Üâ®Ñå´åԄĘǼ~ψΫ|ԫîlӞŞÕ»å´å~ɫųʤ[´âÅ¤ Ӕ  ˘  ^ | l  q  | ն  ® Î Þ £ Þ ® Ȫ γ | ¨ ½ â _ ˬ Ԃ Ƃ h  q TAP մ Tandem Affinity
Purificationյ´¥Ҍƣjq AtrR ψΫhnն҆Űŷ\wͥɿƂhnq AtrR yϓăĥγl´âÅ¤ӔËÞµâ͜|twƋՂұqշƋՂjq´âÅ¤ӔÒ®®Î¤¼àÕ¼Ýåҧ̅jqyfն
HSP70 |ŤՖh´âÅ¤Ӕ_ AtrR yЬƣlƞњɿ_ϥƷhqշţѰԒ͆|]ZwնHSP70 xY
SSZ1 _ PDR1 yЬƣjw҆ŰѐɿĐîjwZfyնq PDR3 _ HSP70 ~ SSA1/SSA2 |Ӈ|ūɱ
hwZfy_˘^|{twZշČǅƥȉhq HSP70  SSA1 ~å²à¥xYնAtrR yf~
SSA1 å²à¥_ϓăĥγjwZfy_Āʊhqնo~ƞњɿ|uZwҳЦ|ҧ̅ҎtwZշ
Screening for the factor(s) that interacts with the transcription activator AtrR involved in ABC transporter gene
expression
Ayumi Ohba, Mizuki Tanaka, Shintani Takahiro, Katsuya Gomi
(Div.Biosci.Biotechnol.Future Bioind., Grad.Sch.Agric.Sci., Tohoku Univ.)

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A. nidulans ~ AmyR  ¥ Þ ¨ å ® Ǿ Ǐ ì ~ ® º Ý ¥ Ò ¼ ¬ ® ¶ â ~ α ƣ ʒ  ʜ ū l ë˼˩čնթϪș˝նɻ͋ŌĈն̜ȣĲčնեӂϒ̲ մЈ͞ǫԲäαƱΰǢյAmyR նΌЕÒÞ¼å®|ɼЉjw<-ÓÜå±Ї~Óàå®ŤҧНԒУԄĘǼщ~ӞŞͥɿƂlӞŞǆǼyjwϛQʓRն¥Þ¨å®ˬȠǗǑմGMMյ|]ZwնA. nidulans ~ AmyR ԄĘǼ~ԄĘǼϠǥ̓մΔamyRյ_ԙἀ|͈ն Ç͇®ºÝ¥Ò¼¬®¶âմSTյǫԚ|αβlfyҠţjqQST αƣʒԄĘǼщ~ψΫӞŞǆǼ AflR |twҵȞhQDamyR x AflR ] STαƣʒԒУ~ԄĘǼψΫßÍÞ_ë˖jqQçˎն¥Þ¨å®Ēǩ~ΓУͽγZwǗ՜jqǞƣնԙἀy DamyR ~ ST αβԚնAflR ] ST αƣʒԒУ~ԄĘǼψΫßÍÞƥЇxYtqQ
GMM |]Zwʹŷl¥Þ¨å®Ύɗǣŷhny ST ~αʒԚ_ĞìjqQA. nidulans ~ ´ÐÜ¼ʜūӞŞǆǼ CreA _ҵȞlQCreA ~ԄĘǼϠǥ̓ ST ǫԚ|αβjqQqնAflR ԄĘǼ~ËàÖå´åՑǖ| CreA ~ЬƣԎŦ_ǾǏjqQf~fy^PCreA _ AflR ~ԄĘǼψΫʜūlfy|tw ST αƣʒ_ʜūhfy_ю\qQĒë~Ь̈^նDamyR x AmyR ~̶ʷ|twģ^~Õ ¿¯Ô|¥Þ¨å®đҾ_̺ɉ|ҎmնЦјŚ~¥Þ¨å®_̊ͶjqΥʏy{նCreA | ´ÐÜ¼ʜū_ҧԳhնAflR ~ψΫ_ͥɿƂj ST _ǫԚ|αʒhqƞњɿ_ю\Q
DamyR ԙἀy͈ GMM ëxǪb~ ST αβlyy|նo~αі_ԌŤϋ|ʜūhQqնÓàå®ŤҧНԒУԄĘǼщ~çԌ GMM γZqǗ՜xψΫjնo~ԄĘǼ~ψΫ_
AmyR |twͥɿƂhfy DNA Ò¤àßҧ̅|ϥjwZQf~Óàå®ŤҧНԒУԄĘǼщ_ AmyR |˵ϛ~¥Þ¨å®đҾūɱ|ԫƞњɿю\wZQ
Transcription factor AmyR of A.nidulans represses sterigmatocystin biosynthesis on glucose minimal medium.
Yosuke Kamimura, Toshiaki Narukami, Motoyuki Shimizu, Shunsuke Masuo, Naoki Takaya(Graduate School of Life and Environmental Sciences, Univ. of Tsukuba)

P-68լѺ|]d¨­ԓαβԄĘǼ~ҧ̅ĠԙŌ˝ǚ˷ŏǼȟ͐Ѳ˘ ǫȴ̑͘ ϾѭǍ͘ ͈ǀѫƽ ,ηδԿù ,ǫЍĴçմԛ͕Ⱦǫ βкϟ êϔҚ҆յ
VϑϋW ¨­ԓլѺ(Aspergillus oryzae)xαβhĂ̷đҾβΣx,¶à¬¾å±ͥɿԮȍlfy|ÕÜ¿â~αʒʜūlQ¨­ԓ¥Þ¨å®^ǧʴhyю\w],o~çԌ~Ԅ
ĘǼ|uZwˬӪ~ϟϹx˘^y{tq1)QofxČǅʓR,¨­ԓαβЫӚ~ŒĢň˘^ylq,DNAÒ¤àßҧ̅|ҧ̅llqQVˎ͜yЬ̈W ĒŮ~ϟϹx˘^y{tq¨­ԓαβūɱlԄĘǼ~եψΫ̓yϠǥ̓γZw
DNAÒ¤àßҧ̅ҎZ,¨­ԓαβ|ԫԄĘǼ~ʣţҎtqQĹҙ|Y_tqԄĘǼA.
oryzae;ligD;pyrG̓Ȑö|jwԄĘǼϠǥ̓ĥҚjqQo~Ь̈,ջu~ԄĘǼx¨­ԓαβԚ~͸Ƞ_ϢҴh,¨­ԓαβ|ԫîlԄĘǼxYfy_ϢҴhqQf~ԄĘǼ|uZwҳЦ{ҧ̅ΫǏҎtwZQ

 Terabayashi et al.: Fungal. Genet. Biol. (2010) 47, p953-61.
Analysis of kojic acid synthesis genes from Aspergillus oryzae
Motoaki Sano, Mistuko Dohmoto, Hideaki Koike1, Eiji Ohsima2, Kuniharu Tachibana2, Yoshitaka Higa2,
Masayuki Machida1, Shinichi Ohashi(KIT, 1AIST, 2Sansho Seiyaku Co., Ltd)

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Aspergillus niger NRRL 328 ὲ III ǔÑÝ¦´½ƣʒԒУ|ÈàâƂƣΣ~αʒΏǑӿýնȌąɅȊնȟ̇ʐçն˷δҘ̲ն̚˼ŏǭԋ մ˔ǫäέȾäɼƂյ
VϑϋW̤Σ|]Zwնˮγ{Ă̷đҾβΣxYÉÜÐÂ½~դ̖ III ǔÑÝ¦´½ƣʒԒУ(PKS)|ɦʒhQIII ǔ PKS ̤Σ|Τξϋ|ǾǏlyю\wZq_նМΥѺ Aspergillus oryzae |]ZwÞ°ÝâԓƣʒԒУ CsyA ¨å½l csyA ~ǾǏ_ϢҴhն§ÂÔҧ̅| A. niger NRRL
328 |]Zw CsyA yÓÂԓԎŦx 36ճ~ϓƥɿϥl̴њ˵ϛ~ III ǔ PKS ÏÖà¥¨å½lԄĘǼ An-csyA ~ǾǏ_ҠţhqQofxնAn-cysA ~¤àå¿â¥]o~ԄĘǼβΣ An-CsyA ~̴њҧ̅jqQ
Vˎ͜]Ь̈WA. niger NRRL 328 ʣţjq mRNA Ԡǔyjq RT-PCR | An-CsyA ¨å½l cDNA ¤àå¿â¥jնǫѠѺȐöyjw His-tag ӶЬjqЪʴ\ An-CsyA αβhnqQЙҚ
jqЪʴ\ An-CsyAնěԧǘӔÒà¿Þ CoAնԩǷǘӔyjwΓУˆ 2ք20 ~ћѕԓ¬Þ CoA γZq
ƘɼҎZնƘɼͭ~ԑԓ¶ÞʣţΣ LC-MS/MS |Ť̅jqQAn-CsyA նΓУˆ 2ք12 ~ћѕԓ¬Þ CoA ԩǷǘӔyjw 2ք5 ǅ~рƣƘɼ|ÈàâƂƣΣαʒƞњxYfy˘^|jqQ͈ӡȘΛyjwնA. oryzae ὲ~ CsyA ~̴њҧ̅ҎZնCsyA _ΓУˆ 2ք14 ~ћѕԓ¬Þ CoAԩǷǘӔyjw 2ք3 ǅ~рƣƘɼ|ÈàâƂƣΣαʒƞњxYfyϢҴjqQĒëնAnCsyA  CsyA yǘӔΤξɿ~ξ{ˍҡ{ III ǔ PKS xYfy˘^|jqQ
Production of pyrone compounds by a type III polyketide synthase from Aspergillus niger NRRL 328
Tatsuya Hamachi, Nozomi Miyai, Keiichi Kobayashi, Yuki Honda, Kohtaro Kirimura(Dept. Appl. Chem., Fac. Sci. Eng., Waseda Univ.)P-70
լѺ A. oryzae ὲ´Ë III ÑÝ¦´½ƣʒԒУԄĘǼ csyB ~̴њҧ̅ϝδέш 1ն̳Ō Ҷ 1նſSɚđ 1նƃ˷žf 2ն҇ą ƀ 1
մ1 ȯʘƅϯǫä҆, 2 ̂ǫԲäӧαϯäɼαȾյ´Ë III ǔÑÝ¦´½ƣʒԒУմPKSյԄĘǼ̤ΣxǪbҠţhwZ_նˬӪ~§ÂÔҧ̅|МΥѺ|ǾǏlfy_˘^|{twZQʓRլѺ A. oryzae ὲ~ 4 ϵՖ~´Ë III ǔ PKS
ԄĘǼ~[sնcsyB ԄĘǼ amyB ËàÖå´åì|ЪөrψΫÍ¤´åĥҚjնA. oryzae M-2-3 ̓ɦӔӞʴjqyfնo~ɦӔӞʴĢ~Ǘ՜ͭô|α-pyrone դ̖uˍҡƂƣΣ csypyrone B1 ~αβϢҴjqQf~Ǘ՜ͭô|h| 2 ϵՖ~Ò¾åʒŤմcsypyrone B2, B3յ_ϢҴhwZfy^նČǅo~̫ӵҧ̅ҎtqQ
ɦӔӞʴĢҵȞǗ՜ͭԑԓ¶ÞxʣţɮնODS ¬Ý§ÞÜÔ¤àÒ¼¥ÜÉå] ODS-

HPLC ЙҚхӫlfy|Ǘ՜ͭ 1.5 L ^ csypyrone B2 մ4.3 mgյ] B3մ2.8 mgյɰqQƢϵ̴ǂŤ̅| B2  csypyrone B1 ľԥ_ 1 Õ¶ßâŤԧZՖоĢնB3  2 Õ¶ßâŤԧZՖоĢxYfy˘^|jqQf~ƂƣΣƥk´Ë III ǔ PKS xĥ germicidin y̫ӵՖоĢxYնαƣʒϋ|ѨƯͰZfy^նΫǏն13C ̮ҿƂƣΣ~ʝîȊգ| csypyrone B Ֆ~ƚөԌĝ˘^|lyy|PαƣʒЫӚ~ҧ̅ӷwZQ
Functional analysis of type III polyketide synthase gene csyB from A. oryzae
Satomi Ishida, Makoto Hashimoto, Yasuyo Seshime, Katsuhiko Kitamoto, Isao Fujii(1School of Pharmacy, Iwate Medical Univ., 2Dept. of Biotechnol., Univ. of Tokyo)

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Pyripyropene αƣʒ|ԫ Pyr1 ~̫ӵ̴њҧ̅ìȺѫɧն̢δ͢Ҏ, ԯԌԊ˱ մ̂ǫԲ҆յ
Pyripyropene A եћҍσ͘υ҆yjwѤɓɼγ_˳ɭhÕàºÞÎÂ½ƂƣΣxYQɥϟϹȋ
xP§ÂÔ»å´Íå®^ Pyripyropene αƣʒԄĘǼ¤Ü®´å_ҠţhPAspergillus oryzae Ȑöyjw¤Ü®´å̫ʒԄĘǼ~ξϵψΫ_ҎqQPyripyropene A αƣʒ|ԫ CoA ligase ÏÖà¥(Pyr1)P]ÑÝ¦´½ƣʒԒУ(Pyr2)~ŕψΫ~Ь̈P¿¨¶âԓԩǷǘӔyjq Pyripyropene A ŮաĢ~αʒ_ϢҴhqQfx¿¨¶âԓ˷̀~ǘӔyl CoA Ý¡å±~ǝƭĩΖbPPyr1 ~ǘӔΤξɿЇ~ԒУȀϋΤɿ|ѨƯ_ʧqqQofxPǫѠѺ|]ZwξϵψΫjqЪʴ\ԒУγZw
in vitro x~ Pyr1 ~̴њҧ̅ҎtqQ
Aspergillus fumigatus F37 ̓^ Pyr1 ~ cDNA ƚɰɮPGST Ҍƣ´âÅ¤yjwǫѠѺ|ξϵψΫhn
qQCoA Ý¡å±PЅçǘӔxY ÞÐâԓ ATP |ͥɿƂjqɮPЅĂǘӔxY CoA y
AMP ôԪĢy~®ºÞćʴƘɼҎ[QofxԒУͥɿ~̥ҫPƘɼűαʒΣxY AMP αʒԚʨ̮yjqQЅçǘӔxY ÞÐâԓ~Τξɿ̥ҫjqЬ̈PPyr1 ¿¨¶âԓrdx{bPÈ¨ÝâԓȄʃ՟ԓǘӔyjwҴҿjqQçˎxPЅĂǘӔ~̥ҫx˷̀~ǘӔxY CoA ƛdőmPacyl
career protein (ACP)~Ï®ÏÅâººâåÔŎЀ̫ӵxY N-acetylcysteamine (NAC)ƛdőfy_˘^|{tqQΫǏPÏÖà­åÖ»Þ|ǘvZqԌĝΤξϋǧξȞő|PǘӔҴҿ̴̫~˪{ҧ˘ϑʨjwZQ
Structure functional analysis of Pyr1 involved in the biosynthesis of Pyripyropene
Yoshihiko Shimokawa, Hiroyuki Morita, Ikuro Abe(Graduate School of Pharmaceutical Science, The University of Tokyo)

P-72МΥѺὲÕàºÞÎÂ½ terretonin αƣʒԄĘǼ~̴њҧ̅̃δĪǫ 1նĖ҇ȵ˅ նɵ̸͌ý 1ն҇ąƀ 2նøǕƶǮ 1նͪэǠӄ 1նԯԌԊ˱ 1 մ1 ̂ǫԲä҆ն2 ȯʘƅǫä҆յAspergillus terreus ~αβl terretonin ÑÝ¦´½y farnesyl ǘὲ~ºÞÎÂ½̫ӵħnʧuΤξ{ÃÊÝ¸½ǔƂƣΣxYQМΥѺὲÕàºÞÎÂ½ƂƣΣ~ô|ՕҀ{αΣͥɿˮl
~_Yնo~αƣʒԄĘǼ~ҧ˘նČɮ~Ŵ҆ϑʨjqΣӔαβ|]ZwԘҞxYQˬӪն˷ƂƣΣ~ՖоĢxY pyripyropene ~ǘ˷դ̖̫АlԄĘǼщ_ƥȉhq_նterretonin ~αƣʒ|]Zw pyripyropene yƥ̬|նÑÝ¦´½ƣʒԒУմPKSյնËß¿ÞǘӞϱԒУմPTյնÉÜÇâƪˮÖÂ¢¬§¾å±մFMOյնºÞÎâΰƂԒУմCYCյ|twǘ˷դ̖_̫Аhyю\QA.
terreus ~§ÂÔë|Ҡţhq terretonin ~ʱȉαƣʒԄĘǼ¤Ü®´åô|նPKSնPTնFMOնCYC ԄĘǼ_ƪնlx|˷¤Ü®´åô~ PKSնPTնFMO ԄĘǼ|ĀʊαƣʒôԪĢ~αʒϢҴjw
ZQČǅնh| CYC ԄĘǼ~̴њҧ̅~qնPTնFMOնCYC ~ 3 ԄĘǼŕψΫНξϵМΥѺ A.
oryzae |w̫АjնPKS βΣʹŷjqǗǑ|wҵȞǗ՜lfyxΰƂβΣ~αʒұq_նCYC Τξϋ{ƂƣΣ~αβϢҴlfyx`{^tqQofxնΰƂƘɼ_ӷҎlĒŮ|նď~ĵ՛ԒУ_Ňbƞњɿю\ն¤Ü®´åô|ǾǏjwZÕ¶ÞǘӞϱԒУմMTյ|ϘϑjqQA. oryzae |w PKSնPTնMT ŕψΫН̫Аjնo~đҾΣŤ̅jqyf PKS ] PT βΣ~Õ¶Þ®ºÞĢ~αʒ_ϢҴhqQΫǏնf~Õ¶Þ®ºÞ_ FMO y CYC |twΰƂĢyǧʴh^Ʃ^̥ҫjw
ZQ
Functional analysis of the biosynthetic genes involved in the biosynthesis of a fungal meroterpenoid terretonin
Yudai Matsuda1, Takayuki Itoh1, Kinya Tokunaga1, Isao Fujii2, Tetsuo Kushiro1, Yutaka Ebizuka1, Ikuro Abe1
(1Graduate School of Pharmaceutical Sciences, Univ. of Tokyo, 2School of Pharmacy, Iwate Medical Univ.)

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2-«â¼Ü¢ÂâαƣʒԒУ~ƥȉy̴њҧ̅ŷ͘ʥƴնͯȺǿъ, ԯԌԊ˱ մ̂ǫԲä҆յ
2-«â¼Ü¢ÂâÑÝ¦´½|ὲlÌºàѮ՟ːƂƣΣxYМΥѺǑҒՖ|βαh
Qf~[{ƂƣΣʞ_ͥɿʞЬ̔ĥγ{z~Ǫ̬{αέͥɿϥlā_ϛwZQ2-«â¼Ü¢Ââդ̖ Wagoner | Scorpinone ~αƣʒϟϹ|]Zw, ƥĝĢʝîȊգ|ÌË´¦´½ôԪĢ^αƣʒhfy_˘^|hwZ 1QαƣʒôԪĢyαʒΣ~̫ӵ^Ũˌlyf~ƂƣΣ ACP ^Þ»Æ½yjwҧՂlfy_ĀʊhwZqQofx,¶ßµ¤´å±(R)½Õâˮl type I PKS αƣʒԒУyjw͟ϑjqQ
fx| Fusarium solani |]Zw 2-«â¼Ü¢ÂâxY bostrycoidin _βαhā_ǝƭhwZQofx F. solani ~§ÂÔ|Șjw 3-methylorcinaldehyde ƣʒԒУ~ R ½Õâ~ÓÂԓԎŦγZqϓƥɿ̥ФҎtqyf, R ½Õâʧu type I PKS (bos1)y Adenosine deaminase like protein (bos2)ƪαƣʒԄĘǼ¤Ü®´å_ҠZrhqQBos1 _Þ»Æ½ʧuÌË´¦´½ôԪĢƣʒj,
Bos2 _ÓÂǘӞϱԒУyjwŇZw«â¼Ü¢Ââդ̖ƣʒlyĀʊjqQ
ջu~ԄĘǼ bos1 y bos2  Aspergius oryzae M-2-3 ̓xξϵψΫhnāx, ˷ԄĘǼ¤Ü®´å_ 2-«â¼Ü¢Ââդ̖~αƣʒ|ԫ^Ʃ^˘^|j, q in vitro xԒУƘɼҎZ, op~ԒУ̴њ˘^|lĀȉxYQ
1: Van Wagoner, R.M. et al. J. Nat. Prod. (2008) 71, 426-430
Biosynthesis of 2-Azaanthraquinone
Takuya Kaji, Takayoshi Awakawa, Ikuro Abe(Dept. of Pharmaceutical Sciences, Univ. of Tokyo)

P-74
ŤǼŚ peroxide ̳̋ɦʒҨǻl Verruculogen ƣʒԒУ FtmF
ŷ҇ϒ̲ 1նǳ˼ѲǮ 2նե̳ĲĂ 1նԧδҘù 1 մ1 έϟǘɏϟä¦ÓÞÄà­åն2 եӤɗŏϯȀϟϹ°â´åյÒ¨¼¢¬â verruculogen  Aspergillus fumigatus |]d fumitremorgin αƣʒЫӚ~ˬЩβΣ~çu
xYնŤǼŚ| peroxide ̳̋ˮlΤɶϋ{̫ӵˮjwZQʓR~fx~ fumitremorgin αƣʒԄĘǼ¤Ü®´å~ҧ̅^նα−¦¼¥Þ´ÞԓĬǾǔ­¢¬§¾å±yϓƥɿϥlԒУԄĘǼ
ftmF _նo~αʒ|ԫîjwZfy˘^|jwZQFtmF ~ҨǻlŤǼŚ peroxide ̳̋ɦʒƘɼ~Õ¿¯Ô~έҧϑϋ|ն˷ϟϹx FtmF ~̴њҧ̅{|Ь˥̫ӵҧ̅ҎtqQ

ǫѠѺ|]Zw His ´¥´âÅ¤ӔyjwψΫhnqЪʴ\ FtmF նα-¦¼¥Þ´ÞԓնFe2+âն®¨ÞÇâԓǾǏìxնfumitremorgin B yƘɼhnqyfնverruculogen ~αʒ_ϢҴx`qQˬԂƂjqƘɼ˿Ĕ|]Zwնfumitremorgin B ]α−¦¼¥Þ´Þԓ|ȘlƘɼŻŵȀȉˆ~ͻȉҎtqQ
qնҁ͊ʦ˃͜|wЙҚ´âÅ¤Ӕ~Ь˥Ƃ˿ĔʯtqyfնPEG4000 ͒̈́Űyjq˿ĔxЬ˥_ɰqQf~Ь˥γZw X мǅʟȊգҎtqyfն1.82Å Ťҧњ|w FtmF ~Ь˥̫ӵ˘^|lfy_ţ̀qQFtmF Ь˥ô|]ZwµÒå̫ӵytw]PFtmF _Ȫl Clavaminate
synthase-like ®åÅåÉÓÝå|]ZwbĳǾhwZ double-stranded β-helix ̫ӵˮjwZqQ
Functional characterization of verruculogen synthase FtmF of Aspergillus fumigatus
Naoki Kato1, Hideo Okumura2, Shunji Takahashi1, Hiroyuki Osada1(1Chemical Biology Dept., RIKEN ASI, 2JASRI)

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Þ¬Ü¤´âαƣʒԄĘǼ¤Ü®´å~ҧ̅δôɩ 1, 2ն˷ȬեɎ 1ն̇ˁ˘ 1նɜδ͢ 1նȄ҇ϒǼ 2նԧδҘù 1մ1 έϟä¦ÓÞÄà­åն2 ̂͢ǫäȾյVϑϋWÞ¬Ü¤´âМΥѺ Fusarium sp. RK97-94 ̓|twαβhĞŤǼƂƣΣxYն_ʜūԄĘǼ p53 íͥƂЦјxЦјƮ˳ԮȍͥɿϥlQp53 ԄĘǼЦјƮ˳~¨â¼àåÞ{z|ԫîjw]նǪϵ~џτ|]Zw̶ʷնYZǧξӗfjwZQtwնp53 ԄĘǼ_ԫîj{ZЦјƮ˳~ļ̹նƔ p53 ǧξĢ~ϾĢ̫ӵǧƂ|ͥɿƂҎ[ƂƣΣʞ_Űyjw˳ɭx`Qo~qնÞ¬Ü¤´âʞ_Ű~Ýå½ƂƣΣyjw˳ɭhQofxնαƣʒЫӚ~ҧ˘yնαƣʒԄĘǼʾǧ|ˍҡեͥɿՖоĢƚɰϑϋ|նÞ¬Ü¤´âαƣʒԄĘǼ¤Ü®´å~ƋՂҎtqQ
Vˎ͜yЬ̈WÞ¬Ü¤´âնo~̫ӵ_ Fusarium sp.|twαβh^͇xYÉ«Ýâ C yՖĜjwZQÉ«Ýâ C  PKS-NRPS hybrid ԒУxY FUSS |twαƣʒhQo~qնÞ¬Ü¤´âƥ̬~ԒУ|twαƣʒhyю\qQofxնPKS |ɹՐxY AT ½Õâ~ÓÂԓԎŦʉǝγZwËÜÒåĥҚjPԄĘǼ¤àå¿â¥ҎtqQo~Ь̈PFUSS yեZϓƥɿϥlԎŦɰqQ̷|նAT ½Õâ̶ǰhnqԄĘǼϠǥ̓ĥҚjqyfնÞ¬Ü¤´â~αβњȅŒ|ǰtqQ˪|նGenome Walking ͜|twf~ԄĘǼˌΡ~ƮӨՑǖ¤àå¿â¥jնԎŦ͑ȉҎtqQo~Ь̈նαƣʒԄĘǼ¤Ü®´åyʱȉhԄĘǼԎŦ~ƚɰ|ʒŶjqQqնʱȉhαƣʒԄĘǼ¤Ü®´åŚ~ methyltransferase ÏÖà¥~ԄĘǼϠǥ̓ĥʒjqyfնÞ¬Ü¤´â~ѝÕ¶ÞĢαβl[|{նαƣʒ~ԫî_˘^|{tqQ
Analysis of the lucilactaene biosynthetic gene cluster
Akira Tanaka1, 2, Takayuki Motoyama1, Toshiaki Hayashi1, Hiroshi Hirota1, Naoko Takahashi-Ando2, Hiroyuki Osada1(1Chemical Biology Dept., RIKENն2Graduate School of Engineering, Univ. of Toyo)

P-76
МΥѺ Coleophoma empetri F-11899 ~ FR901379 Պαβǧξ̓~ T-DNA ʫőǧξЋʖ~ҧ̅ǢąƇĦǼնȬδԿĊնфԙԴčնϣӆ͠ɠն̳˷̺͘ մȓȬϕǫäαΣȾյМΥѺ Coleophoma empetri F-11899 ʞϖѺͥɿΣӔ FR901379 αβlQf~ FR901379 αƣʒԄĘǼ~ƋՂϑϋyjw ATMT ͜|ʫőǧξҎZնFR901379 Պαβ̓ƋՂjqQo~[s~ 1 ̓x
Y 77-6D ǧξ̓ª«âҧ̅~Ь̈ն§ÂÔ DNA |ʫőhq T-DNA  2 ķyʱȉhqQj^jն
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qQf~fy^ 77-6D ǧξ̓ T-DNA ~ʫő~Ի|§ÂÔ DNA ~ǫ`{̶ǰնqԄĘǼɘ~Ӟɘ_ӗ`qyю\qQofxն77-6D ǧξ̓~§ÂÔ DNA |z~[{ǧξ_ӗ`q~^ PCR yª«âҧ̅|ҺqQ4 Ћʖ~ʫőǧξЋʖxopËÜÒåĥʒjնЪƣnǧ\w PCR ǣɊҎtqQqնԙἀy 77-6D ǧξ̓~§ÂÔ DNA  Cla I y Eco RV xͬƂjն4 Ћʖ~ʫőǧξЋʖxopĥʒjqËàåÊγZwª«âҧ̅j͈ӡҎtqQ
Investigation of inserted T-DNA regions in FR901379 non-producing mutant of fungus Coleophoma empetri F11899
Chikako Sakai, Masato Yamada, Yhosuke Orino, Yasuhiro Isogai, Seiji Hashimoto(Dept. of Biotechnol., Toyama Pref. Univ.)

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ϖѺ|]dà¢¬®àÒ¬â~ˍҡĥγΔ~ʯФϝą ˥նΝǒ Ѵġնȟ͚ ŊɅնƢŽ ġƴնԤĶ եɻ (̂έǫԲέȾäɼαϯ)ϖѺxYÁZsςѺ(Magnaporthe oryzae)Á|ʎ̍l̤ΣςƐМΥѺxYնΫǏxŒðιxǪb~ҔȍţjwZyy|̤ΣςƐМΥѺ~Ö»ÞαΣyjwԘҞҢhwZQ˷Ѻʎ̍˞|јǼ^ψѰЌějնo~Ŏ|ĐϘǂyư½åÔΥ~ǂȇɦʒjն̤ΣĢŚ|ĮőlQf~ZsςѺ|Șjw̬R{ʞαΣӔĥγhnqyf 14 ƵΰÒ¤àÜ½НʞαΣӔxYà¢¬®
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A Possible alternative Target of Roxithromycin in Fungi
Akira IshiiնMayu KumasakaնYuuki KoizumiնYuuya KakumuնTakashi Kamakura(Tokyo Univ. of science)P-78ÁZsςѺ~¢¶â»°¶Üå±ϓƥ´âÅ¤ԄĘǼ CBP1,CBL1 ~̴њҧ̅ƤδыնǫԙŊǼնôȹġçնԤĶեɻ մ̂έǫäɼαϯյÁZsςѺ Magnaporthe oryzae Ȑö̤ΣŚ~Įő|]ZwĐϘǂyưʎ̍ΤξϋǂȇɦʒlQf~ĐϘǂɦʒնʰϘғՋ~ϡɗնο͋ɿɗ{z~Σέϋ¬¥¾ÞնqƂȀҵȞǆǼ|twҵȞhfy_^twZ_Po~ҳЦ{ŤǼ̴̫˵ϛ{ԌŤ_ǪZQo~ŤǼ̴̫ҧ˘lfyϑϋ|նĐϘǂɦʒҵȞ˞|]ZwեZψΫΤξɿϥlԄĘǼyjw¢¶â»°¶Üå±ϓƥ´âÅ¤ԄĘǼ CBP1(chitin binding protein 1)ƋՂjqQΣέϋ¬¥¾Þ~_ǾǏlο͋ɿĊȾǋĢǘӔë|]ZwնCBP1 ԄĘǼϠǥ̓~ĐϘǂɦʒΪՕҀ|Ğìjq_նƂȀҵȞǆǼ~ʹŷ|twԙἀyƥЇxĐϘǂɦʒΪ_ǅɲjqfy^նCBP1 Σέϋ¬¥¾Þ~Ҵҿ|ԫîlyю\qQh
|նCBP1 y½ÕâßÍÞxϓƥɿ~եZԄĘǼ CBL1 (CBP1 like gene 1)ψҠնÂ¸¤¼Ҏt
qQCBL1 ԄĘǼϠǥ̓ԙἀyǧ}ɦӔxYtq_նCBP1CBL1 ԄĘǼĂԘϠǥ̓ CBP1 ԄĘǼϠǥ̓h|ĞZĐϘǂɦʒΪϥjնƂȀҵȞǆǼʹŷjwĐϘǂɦʒΪȅŒ|ǅɲj{^tqqնCBL1 _ƂȀ¬¥¾Þ~Ҵҿ|ԫîjwZƞњɿ_ϥƷhqQj^jն̨Ҥ͋ɿĊȾǋĢғՋëxƂȀҵȞǆǼǾǏìxԙɿ̓y CBL1 ԄĘǼϠǥ̓_ĐϘǂɦʒl~|ȘjնCBP1 ԄĘǼϠǥ̓ CBP1CBL1 ԄĘǼĂԘϠǥ̓xĐϘǂ yzɦʒj{^tqQf~Ь̈^նĐϘǂɦʒ|]d CBP1նCBL1 ~ĥγ̴ĥĀʊқՁxYyю\նo~ҧ˘|ƧdwΫǏҧ̅ҎtwZQ
The role of Magnaporthe oryzae Chitin-Binding Protein Genes, CBP1 and CBL1, in Appressorium Differentiation
Sho Yoshida, Yuko Ohno, Yuichi Nakajima, Takashi Kamakura(Dept. of Applied Biological Sci., Tokyo Univ. of Science)

77

P-79
ÁZsςѺ~ψѰЌψΫԄĘǼÜÊÜÝå¤àåâ B51 ~ԄĘǼϠǥĢҧ̅ĠR˴Ľʅնȟ͚ŊɅƢŽġƴ͵δơԤĶեɻ ÁZsςѺ Magnaporthe oryzae ÁZsςɟ`ӗfl̤ΣςƐМΥѺxYQ˷Ѻ~ŤαǼ̤ΣғՋë|ĐϘjqɮψѰЌěԧjo~ŎЀ|ɦʒhʎ̍ΤξϋǂȇxYĐϘǂ^ĮőМělfyx̤ΣĢŚ|ĮőlQÁZsςѺ~ʎ̍ӽϳ|]dĐϘǂŤƂϖ̔αΣ~ɦʏŤƂςȍԭԳyZtqҥΔ|]ZwՊɉ|ѨƯͰZΫӅxYfy^˷ϟϹȋxψѰЌěԧ˞|ψΫlԄĘǼ~ҧ̅ҎtwZQ
ʓR~¥ÞåËψΫԄĘǼÜÊÜÝå~ôx, Τ|¤àåâ B51 |ϘϑjwϟϹӷwZQ
B51  DNA ĵɲЦјƮ˳ūɱӞŞūɱ{z|ԫîl FHA ½Õâʧuyю\wZQɥϟϹȋxĥҚjq B51 Ϡǥ̓|]Zw˘^{ѺМʒԧӴɗĞì_ϢҴhyy|, ψѰΪ]ĐϘǂɦʒΪ~Ğì_qQŷ\wŤαǼ~ɦʏ̔ˆψѰɦʏ|ԫlξɉ_ŤαǼ_ǪˆҥȖhqQĒëB51 _ÁZsςѺ|]dŤαǼɦʒ, ̔Ťҕūɱ, ψѰūɱ, ĐϘǂɦʒ|]ZwԘҞ{Ň`ʧufy_ϥƷhPΫǏPh{ҧ̅ӷwZQ
Functional analysis of germ tube expressing cDNA library of Magnaporthe oryze
Kengo Sasaki, Yuki Koizumi, Yuya Kakumu, Tsukasa Seida, Takashi Kamakura(Dept. of Applied Biological Sci., Tokyo univ. of Sci.)

P-80
ÁZsςѺ|]dÁʢʞɿҵȞǆǼαβԄĘǼ~ʯФǒƜ̼äƐȴĲ˘äҝ˼խԗ͏ (αΣϟ)
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Screening of genes related to production of an elicitor-like agent in Magnaporthe oryzae.
Ayumu Sakaguchi, Toshiaki Harashima, Marie Nishimura.
(NIAS)78

P-81ÁZsςѺ|]dĊΕϋ DNA Ă˷ԥťˌҵȞyϓƥЪʴ\ĵɲѷʹӏùնǫԗĵçն*ˮ͏ŵնДȬžшն̛δѳ մ˘͘ǫӧä*ӧȾǫӧյÁ|ԘҞςȍɟ`ӗflÁZsςѺ (Magnaprothe oryzae) |ÁƳϵ|ςƐɿ_ξ{ßå®_ǪˆǾǏlQfĥΣyjwіϵhw`qեɗʢʞɿÁƳϵ|Șjwն˷Ѻ_ˍq{ςƐɿΨɰjw`qfyնuˍҡßå®_ţΫjw`qfyʌƯjwZQˍҡßå®~ţΫ˷Ѻ_ĳˮlՊςƐɿԄĘǼ|ǧξ_αkfyxɟ`ӗfhqնʓRo~ǧξ̴̫~çuyjwĢЦјϓƥЪʴ\ (HR) |Ϙϑjնҧ̅ӷw`qQΫǏx|նHR ̥ţγÒå åԄĘǼ˷Ѻ§ÂÔ|Ȟőlfyxն̬R{®¼ß®˿Ĕìʎ̍ӽϳ|]Zwαk HR  YFP ҋŏyjw̥ţlfy|ʒŶjնHR |ՊςƐɿԄĘǼ_ǧξlfyȊүjq (Ɍʒ 22 ɍɗ ˷¨âÉßâ®)Qofxն
HR _Ǫb~αΣϵ|]Zw DNA Ă˷ԥťˌ (DSBs) ~ĵɲ̴̫yjwŇbfy|͟ϑjնǘϤϋ̴̫~ҧ̅Ңԙ|ZqĊΕϋ DSBs ~ҵȞy HR ĵɲ|uZwҰĭ]f{tqQDSBs ~ҵȞ|ն18 ǡǘ
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DSBs _αkqƛȏĢԎŦ_īîĢԎŦԠǔyjq HR |ĵɲhqfyϥl YFP ҋŏҥȖx`qfy^ն˷Ѻ|]Zw I-Sce I ԄĘǼγZqĊΕϋ DSBs ҵȞ_ƞњxYfyնDSBs ĵɲ̴̫yj
w HR _ĥγlfy˘^yjqQInduction of artificial DNA double strand breaks and homologous recombination repair in Rice blast fungus
Takayuki Arazoe, Shuichi Ohsato, * Tsutomu Arie, Katsuyoshi Yoneyama, Shigeru Kuwata(Sch. Agric., Meiji Univ.; *Grad. Sch. Agric. Sci., Tokyo Univ. of Agric. & Tech.)P-82ÁZsςѺ|ʎ̍lÒ¨Þ® MoCV2 y MoCV1 ~αƂȀϋ{͈ӡҧ̅̂ͧ˦ýնͧȬĲçնϬƐˁҎնˮ͏ŵնȗȮɷն̢ȬҘō մӧȾǫԲäӧȀɖյÁZsςѺ Magnaporthe oryzae ~ɢ͇Ƃ̓ S-0412-FEc ̓| 2 ϵՖ~Ò¨Þ®_ͳƣʎ̍jw]PopPMagnaporthe oryzae chrysovirus 2(MoCV2)PMagnaporthe oryzae endogenousvirus
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S-0412-FEa ̓|ʎ̍jwZ Magnaporthe oryzae chrysovirus 1(MoCV1)yՊɉ|եZϓƥɿմ̔ԓ:99%Ē
ëPÓÂԓ:99%Ēëյϥlfy_Ũ˘jqQj^jPMoCV2 _ʎ̍l S-0412-FEc ̓ MoCV1 _ʎ̍l S-0412-FEa ̓y͈ӡjwՕҀ{αіԮȍĥγƛdw]PMoCV2  MoCV1 ɤZɢ͇
ƂĥγˮlPƔPƢȐöѺ~Þ®|Șlʎƛɿ_ξ{fy_ʊȉhqQ
MoCV2 y MoCV1 ~͈ӡҧ̅Ҏ[q|PMoCV2 ~Þ®̬ЖǼ~РƂPÞ®̫ʒ´âÅ¤Ӕ~ҧ̅PŒǡǘԎŦ͑ȉұqQРƂjq MoCV2 Þ®̬ЖǼՅǼՕɴԦxҥȖlyPϒɬП
35nm ~άɦ~ЖǼ_ϢҴhqQqPSDS-PAGE γZwÞ®ὲ´âÅ¤ӔȩԩlyP
130kDaP70kDaP65kDaP58kDa ~ŤǼԚʧu 4 ϵՖ~´âÅ¤Ӕ_̥ţhqQf~ɿӔ|]ZwP
MoCV2 y MoCV1 ~ԪxՕҀ{ϓԀ_Ҵ{^tqQçˎPMoCV2  MoCV1 y͈wȐöѺ|ȘjwɤZαіԮȍϥl_PЦјŚx~Þ®ƪԚП 1/10 ĒìyĞ^tqQČɮP2 ϵ~Þ®Ԫ|]dPαіԮȍÞ®ƪԚ~ϓԀ|uZwҺ̐lQ
Comparison between two related mycoviruses, MoCV2 and MoCV1, infecting Magnaporthe oryzae
Tomoya Higashiura, Syunichi Urayama, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka,
Hiromitsu Moriyama (Faculty of Agriculture, Tokyo Univ. of Agri.  Tech.)79

P-83 (O-13)ÁZsςѺɢ͇ƂÒ¨Þ® MoCV1 ~ɿΥҧ̅ƕÞ®´âÅ¤Ӕ~̴њҧ̅ǭδ˦ǼնͧȬĲçնϬƐˁҎնˮ͏ŵնȗȮɷն̢ȬҘōմ̂ĉӧȾǫԲäӧյMagnaporthe oryzae chrysovirus 1(MoCV1)ÁZsςѺ|ʎ̍jնȐöѺ|αіԮȍɢ͇ƂӗflÒ¨Þ®xն4~5 ŤЎ~Ă˷ԥ RNA §ÂÔˮlάɦ~Þ®xYQMoCV1 |ʎ̍jqÁZsςѺЦјŚxͭј~єǫƂՓЖƂyZtqξɉ{ɦʏΫjն MoCV1 Þ®ͿͭÞ®ÉÝå~ÁZsςѺ|ʹŷlyѺМ~Ѽр_ҠQ
MoCV1 ʎ̍̓~ѺĢƙԚƧëϑϋyjwÓ¿­×åxǗ՜jqЬ̈նǗǑԚն̤ѺԚƕǗ՜˞Ԫ_ƥk˿Ĕx¨ÞÍâγZqʪy[Ǘ՜y͈ӡjwնП 20 ĸϳɗ~ѺĢԚ_ɰqQ̷|ѺĢ^
MoCV1 Þ®ЖǼЙҚjw SDS-PAGE ]f{tqЬ̈ն14 ˒ԪǗ՜jqѺĢὲ~Þ®ʒŤն
2 ˒ԪǗ՜jq~y͈ӡjwնÞ®̫ʒ´âÅ¤Ӕ~ª¯_ȟhb{twZqQɯtwնÞ®
ЖǼ̫ʒl´âÅ¤ӔնǗ՜˳Ԫ_ԧ˳Ƃlyçȉª¯|ŤҧմËà°¸¬â¥յhfy_ϥƷhqQqնÁZsςѺ~ɦʏξɉ|ԫîlÞ®ԄĘǼʯФlұyjwնÅâԒ͆Ūγjq MoCV1 Þ®ԄĘǼψΫН~̫Аyҧ̅ҎtqQo~Ь̈նÞ®´âÅ¤Ӕ~çuնԒ͆Цј|αіíѫ{z~ɦʏξɉɟ`ӗflfy_ϥhqQ
Functional analysis of viral proteins of MoCV1 (Magnaporthe oryzae chrisovirus 1) which confers hypovirlencetraits to rice blast fungus.
Tokomo Ohta, Syunichi Urayama, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka, Hiromitsu Moriyama.
(Dept. Agric., Tokyo Univ. of Agric. & Tech.)P-84ÁZsςѺɢ͇ƂlÒ¨Þ® MoCV3 (Magnaporthe oryzae chrysovirus
3) ~ɿΥҧ̅ӬδСƟäͧȬĲçäզąԅǼäϬƐˁҎäˮ͏ ŵäȗȮ ɷä̢ȬҘōմӧȾǫԲäӧȀɖյÁZsςѺ S-0412-F 2a ̓  ʒŤ~˷ԥ RNAմdsRNAյˮlÒ¨Þ®|ʎ̍jw]ŎҎϟϹ~ӷxZ MoCV1մMagnaporthe oryzae chrysovirus 1յʎ̍̓y͈ӡlyҀjZÞÇÂƂŤαǼɦʒԚ~Ğì{z~αіԮȍϥlQɥҲÞ®MoCV1 yǡǘԎŦ~ϓƥɿϥlfy^
MoCV3մMagnaporthe oryzae chrysovirus 3յyƱƦjqօMoCV3  MoCV1 yƥ̬|ѺՖÞ®yjw
{ɿӔxYЦјǩÉå¯ˮjqQǡƂ°¬ÔȒɗƁԎԁɸ͜|ͩӺȒɗ 1.36-1.39g/ml~θŤ|ǅƙjqРƂÞ®ͿͭϒɬП 35nm ~άΥϥjqօSDS-PAGE xȠ{by 4 ʒŤմP130P70P65P58յ~´âÅ¤Ӕx̫ʒhfy_ϥhqօΫǏѺĢ~ƙԚ¸Ë~q|Ó¿­×åx~ S-0412-F 2a ̓~Ǘ՜˿Ĕ̥ҫjw]Þ®ЖǼ~ƙԚƧëÞ®̫ʒ´âÅ¤Ӕ~ʒŤǧƂ|uZw~ҧ̅|ӒlĀȉxYօCharacterization of MoCV3 (Magnaporthe oryzae chrysovirus 3) that confers hypovirulence to rice blast fungus.
Hirofumi Sakoda, Syunichi Urayama, Ryoko Takai, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka, Hiromitsu
Moriyama(Faculty of Agriculture, Tokyo Univ. of Agri. & Tech)80

P-85 (O-12)
϶ZsςѺ Magnaporthe grisea |]d̤Σ»Éâ¬â~ĥγÕҧ̅¿¯ÔȟկѮƟPȬȶˤéP̟˷̺ùPե˴̺ӾPեø͢˧մˍΈ҆ǫäɼαϯյ̤Σ|ʞѺɿҊϊӔ|αĢԭɱ̴̫_ǾǏlQʓR̤ΣʞѺɿҊϊӔ~ 1 uxY Ü¬¾ὲ»Éâ¬â BJ-AFP1 _P϶ZsςѺ Magnaporthe grisea |ȘjեZʞѺͥɿϥjPo~ԻPЦјѡѝŤ̨ƂPͥɿԓУϵ(ROS)~҂ϸƕѺМ_ÄÞåâ̫ӵ~ɦʒ_ӗffy˘^yjwZ˷ǫėxPBJ-AFP1 ʹŷɮŧ˳~ M. grisea ѺМƔјǼ~ɼЉ|͟ϑjPҧ̅jqЬ̈|uZwǝƭlQ
Ъʴ\Ԓ͆ Pichia pastoris ŪγjPψΫäЙҚjq BJ-AFP1  M. grisea |ʹŷjqyfP15 Ťɮ|ЦјѡѝŤ̨Ƃƕ ROS ~҂ϸ_ӗfP˞ԪЫӽyŕ|ѝŤ̨Ƃ~ųƣƕ ROS ǣŷjqQqPјǼ| BJ-AFP1 ĥγhnqy` 15 ŤxЦјѡѝŤ̨Ƃ_ӗfPh|ӽŲԚ~ BJ-AFP1 ʹŷlfyxјǼ~Ϡҕ_ҥȖhqQqPBJ-AFP1 ʹŷ|ѺМ~ÄÞåâ̫ӵɦʒPեΎɗ BJ-AFP1 |ȘjϜ˞Ԫxɦʒhfy_˘^y{tqQqPËà¼ËÜ®¼ƂjqѺМ| BJ-AFP1 ĥγhnqy`|PBJ-AFP1 ĞʎƛɿϥjqQf~fy^ЦјǤë| BJ-AFP1 ʎƛɿ͑ȉlǆǼ_ǾǏjwZƞњɿ_ϥƷhqQ
The mechanism of action of the plant defensin BJ-AFP1 in Magnaporthe gisea
Yoshifumi Oguro, Harutake Yamazaki, Masayuki Nashimoto, Masamichi Takagi, Hiroaki Takaku(Applied Life Sci, Niigata Univ. of Pharmacy and Applied Life Science)P-86Ágѿ̊ςѺ~ŤαјǼɦʒıӷlМΥѺ Phomopsis sp. (Diaporthe sp.)ǫҝԾčնëԙҶնѷΑ̪ն˴Ɛͱç մȴ̕ǫäαӒյÁgѿ̊ςѺմBipolaris oryzaeյ~ŤαјǼɦʒնӪХǩмմ͞ԧ 300-400 nmյΛȚɮ~˨կì|]ZwҴQČǅնÁgѿ̊ςѺy~ȘȲǗ՜|twնÁgѿ̊ςѺ~ŤαјǼɦʒıӷlМΥѺɰqQ¨à¿å~ɿΥն]նrDNA ~ ITS Ցǖ~ǡǘԎŦ^ն˷МΥѺնPhomopsis
sp. (Diaporthe sp.)xYyʱȖhնD1 Ѻ̓yƦĐdqQȘȲǗ՜ҎtqǞƣն D1 Ѻ̓¨à¿åyʰҨlƮӨxնÁgѿ̊ςѺ~ŤαјǼɦʒ_ˮʌ|ǣŷjqQf~ΫӅնÁgѿ̊ςѺy D1 Ѻ̓~op~ѺМ_ϒʰʰҨjwZ{bwҴqQ̷|նD1 Ѻ̓~Ǘ՜jq PDA ǗǑնѺĢƪrå¼¤ßåÊ΀ѺjնŜɗն¬×åßŚxǋƂhnqǗǑxÁgѿ̊ςѺǗ՜jqЬ̈ն¨â¼àåÞy͈ӡjwնÁgѿ̊ςѺ~ŤαјǼɦʒ_ˮʌ|ǣŷjqQh|նD1 Ѻ̓ƢϵͭĢǗǑxǗ՜ɮնӽ΀ѺjqǗ՜ͭÎåÅå»®¤|΃ìjնf~ÎåÅå»®¤yÁgѿ̊ςѺȘȲǗ՜jqЬ̈ն¨â¼àåÞy͈ӡjwնÁgѿ̊ςѺ~ŤαјǼɦʒ_ˮʌ|ǣŷjqQĒë~Ь̈^նD1 Ѻ̓|twŤ͛hΟȄȉɿ~ģ^~ΣӔ_նÁgѿ̊ςѺ~ŤαјǼɦʒıӷlfy_ю\qQČɮնD1 Ѻ̓γZwնÁgѿ̊ςѺĒǩ~МΥѺ~ŤαјǼɦʒ_ıӷl^Ʃ^|uZwҺ̐lyy|նŤαјǼɦʒıӷlΣӔ~ƋՂäƥȉҎ[ĀȉxYQ
Conidiation-promoting fungus Phomopsis sp. (Diaporthe sp.) against rice brown spot fungus Bipolaris oryzae
Yusuke Ohnishi, Makoto Ueno, Sakae Arase, Junichi Kihara(Fac. Life Env. Sci, Shimane Univ.)

81

P-87
¼Öà¨¬gѿ̊ςѺ (Cochliobolus heterostrophus) ~ NADPH Oxidase ԄĘǼщ~̴њҧ͚̅ͤɠġ*նհ҇ϫçնğδƉýն̢δБնδôƇȜ մĉǫäԲäӧն*Ϋ ΁ӑϕǫäȀʪΤũϟϹƵյ
NADPH Oxidase նαĢŚ|]döҞ{ͥɿԓУ (O2-) βαԒУyjwϛwZշŻΣ̤ΣxնςƐѺ~Įő|Șlԭɱʘ͂yjw NADPH Oxidase ŪγjwZfy_ϛwZշo~çˎxն̤ΣςƐѺՖ NADPH Oxidase ԄĘǼщĳˮjwZշČǅʓRնöҞ̤ΣςƐѺxY¼Öà¨¬gѿ̊ςѺ (Cochliobolus heterostrophus) γZwնNADPH Oxidase ԄĘǼщ~ԄĘǼϠǥ]
̴њҧ̅ҎZն̤ΣςƐѺ|]dɫųҺqշ§ÂÔҧ̅~Ь̈ն¼Öà¨¬gѿ̊ςѺ 3 u~ NADPH Oxidase ԄĘǼ (Nox1, Nox2, Nox3) ĳˮjwZqշNox1 ԄĘǼϠǥ̓xնÕÜ¿âƂ~íŒնјǼɦʒњ~Ğìն]Ǽ~[̓ɦʒ~íŒ{zɦʏɦʒ|]ZwҀjZɪՍ_ҴqշçˎնNox2] Nox3 Ϡǥ̓xf~[{ɦʏɦʒ~ξɉy{^tqշ¼Öà¨¬|ȘlςƐɿұգxնNox2 Ȑö~Įőӽϳ|]ZwҀjZíŒϥjq~|ȘjնNox1 ] Nox3 ~Ϡǥ̓xնςƐɿ~ɤZɪՍy{^tqշNox2 Ϡǥ̓նĮőǂȇxYĐϘǂɦʒlfy_ţ̀~~o~ɮ~Įőӽϳ|ξɉ_ҴqշĒë~Ь̈^ն¼Öà¨¬gѿ̊ςѺ|]Zwնɦʏɦʒö|ūɱl Nox1 ԄĘǼyȐö~Įőӽϳö|ūɱl Nox2 ԄĘǼZ[ĨZŤd_ǾǏlfy_ɤbϥƷhqշ
Characterization of NADPH oxidase genes in Cochliobolus heterostrophus.
*Kosuke Izumitsu, Yoshimoto Saitoh, Takuya Sumita , Atsushi Morita, Chihiro Tanaka(Grad. School of Agriculture, Kyoto Univ., * Research Fellow of the Japan Society for the Promotion of Science)

P-88¼Öà¨¬gѿ̊ςѺ|]d Cdc42 ԄĘǼ~̴њҧ̅ğδƉýն͚ͤɠġ, ̢δБ, δôƇȜմĉǫäԲäӧյ
¼Öà¨¬gѿ̊ςѺ Cochliobolus heterostrophus ¼Öà¨¬~öҞςƐѺ~çuxYP̤ΣςƐɿМΥѺ~Ö»ÞαΣyjwԄĘǼϟϹ_ӷwZQʓRP˷Ѻ~ЦјŚ¬¥¾ÞĘӿЫӚ|uZw~Һ̐Ҏtw`qQfx~Һ̐|PCHK1-MAPK ЫӚy Nox2 ЫӚ|ď~Ǫb~ЫӚyξ{PςƐɿy~͟ϑl`ԫӶɿ_ǾǏlfy_ϥƷhwZQôx CHK1-MAPK ЫӚĐϘǂɦʒkylʎ̍ŧ˳ӽϳūɱjwZyю\Q˷ϟϹxP¼Öà¨¬gѿ̊ςѺ~ʎ̍ŧ˳ӽϳūɱlÒ®´åß£Øßå´˘^|lqPCHK1-MAPK ЫӚ~ëͦǆǼ~ʯФҎtqQţѰԒ͆ Saccharomyces cerevisiae |]ZwPMAPKK ¢¾å± Ste11 ~ÝâԓƂ MAPKKK¢¾å± Ste20 _ūɱlfyPojw Ste20 ~ëͦǆǼyjw Rho ǔ small GTPase Cdc42 _ǾǏlfy_˘^|{twZQ¼Öà¨¬gѿ̊ςѺ~ CHK1-MAPK ЫӚ̫ʒl MAPKK ¢¾å±
ChSte11  Ste11 ~å²à¥xYfy^PʓR Cdc42 ԄĘǼ|ϘϑjP¼Öà¨¬gѿ̊ςѺ^o~å²à¥yʱȉhԄĘǼ ChCdc42 ¤àå¿â¥jPϓƥЪʴ\Ūγjqԙαǔ̓~ԄĘǼϠǥҎtqQo~Ь̈ɰqԄĘǼϠǥ̓ԙαǔ̓y͈ӡjwʒԧ_Ҁjbӹ^tqQqՕɴԦì|]ZwPŤ̉ɮ~ѺМ_ѢΉl{z~ɦʏξɉ_ҥȖhqQh|Pԙαǔ̓yćԎjqyfǼǃ̔ɦʒњ_{^tqQΫǏ̤Σ~Įő|ԘҞ{ĐϘǂɦʒњPȐö|]dςƐɿұգҎtwZQ˷ψғxPf~Ь̈|uZwǝƭjqZQ
Characterization of Cdc42 gene of Cochliobolus heterostrophus
Takuya Sumita, Kosuke Izumitsu, Atsushi Morita, Chihiro Tanaka

82(Grad. School of Agriculture, Kyoto Univ.)P-89ÝՖΓρςѺ_Ť͛lÉ¤´å´âÅ¤Ӕ NIS1 ~̴њҧ̅ő̲̉͠նƤԙ՟вնˡԪ˅նǳԙƶԋնեԙъǿ մĉǫäԲäӧյ ÝՖΓρςѺմ       յÝϯ̤Σ|ςȍɟ`ӗfl̤ΣςƐМΥѺxY
_նÝϯ̤Σ|ŷ\wն       |ʎ̍lfy_ϛwZQʓRնÝՖΓρς
Ѻ_նf~     |ȘlЦј̾ҵȞͥɿˮlÉ¤´å´âÅ¤Ӕ " Ť͛lfy˘^|jwZQČǅն" _̤ΣŐπƘɼʜūx`^̥ҫlq|նÖ»Þ̤ΣxY¬àÀ¾¯¾|]Zwն" ʁɉϋ|ψΫlɦӔӞʴ̤ΣĥţjqQĥţjq " ψΫ¬àÀ¾¯¾նԙαǔ¬àÀ¾¯¾y͈ӡjwնαі~Ğìϥjն" ~ψΫ_¬àÀ¾¯¾~αі|ȘjwԮȍϋŹ̈ƕlfy_˘^y{tqQçˎxնŤ͛¬¥¾Þ̶ǰjq " ψΫhnqǞƣնf~[{ɪՍҥȖhmն" _ÑËÜ®¼yŤ͛hfy|αіԮȍ_ɟ`ӗfhyʱȉhqQf~ " ψΫ¬àÀ¾¯¾|íԂɼǔ~ΓρςѺմ    յʰϵjqЬ̈նʢʞɿ~ҀjZĞì_ҥȖhqQf~Ь̈ն" ~ψΫ|twն¬àÀ¾¯¾~ςƐМΥѺ~ʢʞɿ_Ğìlfy_˘^y{tqQh|նςƐЦѺ    
|Șjwն" ψΫ¬àÀ¾¯¾o~ʢʞɿ~ĞìϥjqQf~Ь̈նÉ¤´å"ն¬àÀ¾¯¾~̤Σʢʞɿʜūl̴њˮlfy_ɤbϥƷhqQΫǏնÝՖΓρςѺ~Ȑö̤Σʎ̍Ëà°®|]d " ´âÅ¤Ӕ~Żʏ˘^|lq|նҋŏ´âÅ¤ӔҌƣ|նŤ͛Ů~ςƐМΥѺЦјŚ]Ť͛ɮ~̤ΣЪф|]dȥǏҧ̅]f{twZQo~Ь̈|uZwħnwǝƭlQ
 {]P˷ϟϹαϟ°â´åTÂÍå¬ÚâŴţǘϤϋϟϹʱӷā̧U~ʶŸƛdwҎqQ
Studies on an effector protein NIS1 secreted by Colletotrichum orbiculare.
Hiroki Irieda, Kae Yoshino, Kei Hiruma, Tetsuro Okuno, and Yoshitaka Takano(Grad. School of Agriculture, Kyoto Univ.)

P-90 (O-14)
ÝՖΓρςѺ~ςƐɿ|ɹՐxYŤ͛´âÅ¤ӔԄĘǼ LAC2 ~ƥȉƕ̴њҧ̅̇ Шĕնǳδ̉Ϸնǳԙƶԋնեԙъǿ մĉǫԲäӧյÝՖΓρςѺմColletotrichum orbiculareյնÝϯ̤Σ|ςȍɟ`ӗfl̤ΣςƐМΥѺxYQ˷ѺնÕÜ¿âƂjqĐϘǂɦʒjwȐö̤ΣĮől_նf~Įőӽϳ|]ZwնĐϘǂ~ÕÜ¿âƂɹՐxYQČǅն˷Ѻ~ςƐɿԫӶԄĘǼyjwնŤ͛ǔÜ¸ å±̬ԄĘǼ LAC2 ƥȉjqQ
LAC2 ~̮ϋϠǥ̓|]ZwնĐϘǂ~ÕÜ¿âƂՕҀ|Ğìjw]P¢ØÝ~ςƐɿǰwZqQGFP γZqßÑå´åҧ̅նLAC2 ԄĘǼĐϘǂɦʒӽϳ|]ZwɤbӞŞҵȞhնo~ьҮβΣնЦјǩŤ͛hfy_ϥƷhqQh|նŤ͛¬¥¾ÞՑǖ̶ǰhnqǧξǔ
LAC2 նlac2 Ϡǥ̓ϓҙx`{^tqQqնlac2 Ϡǥ̓նo~јǼ~Ѭ_ԙἀyξ{twZqQÁZsςѺմMagnaporthe oryzaeյ~ LAC2 ÏÖà¥ÝՖΓρςѺ~ lac2 Ϡǥ̓ȞőjqǞƣնo~ŤαјǼ~Ѭǅɲjq_նςƐɿyĐϘǂ~ÕÜ¿âƂǅɲj{^tqQf~Ь̈նLAC2 |јǼ~ѬУyĐϘǂ~ÕÜ¿âαƣʒնƥçƘɼx{bնLAC2 ~ǘӔƋçx{ZfyϥƷjqQh|f~Ь̈նLAC2 _ԫîlјǼ~ѬУαʒςƐɿ|ɹՐx{ZfyϥjqQ {]ն˷ϟϹαϟ°â´åTÂÍå¬ÚâŴţǘϤϋϟϹʱӷā̧U~ʶŸƛdwҎqQ
Isolation and Functional Analysis of a Secreted Protein Gene LAC2 Required for Fungal Pathogenicity ofColletotrichum orbiculare
ShaoYu Lin, Shiho Okuda, Tetsuro Okuno, Yoshitaka Takano

83(Grad. School of Agriculture, Kyoto Univ.)

P-91 (O-15)
Cryphonectria parasitica RAS3 ´âÅ¤Ӕ~αƂȀäαέȀϋɿӔȬŚŊӤPե̳ʥýPЄƐЧ մȌǕǫä՘β̧äΰǢյ¤Ýљ̊ςմchestnut blightյPðιêǫ̤Σς~պuyjwˮƦxPΤ|ДǌxÅÜ¶ȬǑx̨wŊſxYtq¤ÝմAmerican chestnutյƈðО~Ԫ|āȊëŒ΀hn z~ҔȍqjqQo~ƐǆМΥѺ Cryphonectria parasitica P1900 ɍՎ|_ǌ^¿ØåÛå¤|ӋΣ|ĐϘjwϱőhqyZ[QΫǏPC. parasitica ~§ÂÔҧ̅ Щÿjw]Phi{Ҧɗ^̤Σ~ςɶψΫÕ¿¯Ô~ҧ̅]o~ūɱˎ͜_̥ҫhwZQ
C. parasitica ~ RAS |ԫjwPlx| RAS1PRAS2 _ϢҴhwZ_P˷ϟϹxˍq|Ҡţhq RAS3 |uZwPαƂȀäαέȀϋ̴њҧ̅ҎtqQRAS3 ď~ Ras ԫӶ´âÅ¤Ӕ| ŕӳjwҴ C ˶ЀՑǖ~ÉÞÁ¬ÞƂԌĝմCAAX Boxյ̶bfy_ǫ`{Τɶxնo~ΔxŻΣ~Rin ] Rit y~ŕӳɿ_ҴqQRAS3 ¨å½l ras3 ԄĘǼ~Ϡǥ]ӽŲψΫ_ѺМʒԧŤαǼɦʒ|ƕlɪՍ̥ҫjqQGFP Ҍƣ RAS3 ψΫhnwPo~ȥǏ|uZwҥȖjqyfPЦјғȫմЦјѡëյ|ɤZҋŏ_ҴqQC ˶Ѐ 32 ÓÂԓ̶ǰjq RAS3 Pf~ȥǏϥhmPЦјŚԌŒĢ|Ť˃jwψΫjwZqQf~fy^PC ˶Ѐ 32 ÓÂԓ_ÉÞÁ¬Þǘ|˫ѡЬƣ|ȑîjwZƞњɿ_ϥhqQqPRAS3 ~ GTP Ьƣњ GTPase ͥɿЇ~αƂȀϋɿӔҺq|PǫѠѺôx RAS3 ψΫjPЙҚjqQ
Biochemical and physiological functions of Cryphonectria parasitica RAS3
Yuki Yamauchi, Takuya Takahashi and Shin Kasahara(Dept. of Env. Sci., Miyagi Univ.)

P-92¾¬կˊςѺ~ʎ̍ǂȇ|]dͥɿԓУϵαʒқƣĢ~̴њҧ̢̅δԾçնΩɚͣ, ̢ȺՍǼ, ͐δĽç, ˸˽ŋ մϪʕǫäӧȀϟϹϯյfx|նʎƛɿ¾¬ѿ|ʰϵjq¾¬կˊςѺ~ӍϻѺМƕĐϘǂɕԌ|]ZwͥɿԓУϵ(Reactive Oxygen Species: ROS)_αʒjնROS αʒ|ԫ 2 ¨Èå~ NADPH oxidase (Nox)ԄĘǼ noxAն
noxB ~[sնɮя~Ϡǥǧξ̓|]ZwĮőњŵ]ςˊɦʒњŵ_Ƽǰlfy_˘^y{twZQh|նnox ǧξ̓xѺМŤȭՒɗ~ǣŷ_ҴwZQČǅն˷Ѻ~§ÂÔÜÊÜÝåªÊ¤àå¿â¥jq noxB | gfp ҌƣhnqßÑå´åԄĘǼ noxB Ϡǥǧξ̓|ȞőjնҋŏȥǏɿҺ̐jqQo~Ь̈նßÑå´åԄĘǼȞő̓xĮőњŵ]ςˊɦʒњŵǅɲjqQ°Þàå®
ѡë~јǼψѰĢҋŏՕɴԦҥȖjqyfնGFP ҋŏ_ĐϘǂ|ȥǏjwZqշçˎն̑՜αԧhnq
ѺМxնo~ŒĢ| GFP ҋŏ_ҴqQĒëնʎ̍˞y̑՜αԧ˞xѺМ|]d NoxB ~ψΫäȥǏ̬ɞ_ξ{fy_˘^y{նNoxB _ѺМ~̨ɿαԧ|]ZwԘҞ{ɫų̈qjwZƞњɿ_ϥƷhqQ
The functional analysis of Nox complex in infection structures of Alternaria alternata Japanese pear pathotype
Yuichi Morita, Gang-Su Hyon, Kyoko Morikawa, Ken-ichi Ikeda, and Pyoyun Park
(Dept. of Agriculture, Univ. of Kobe)

84

P-93
լѺ A. oryzae |]d AoSO y Stress Granule ~ŕȥǏҧ̅ծ ͼǺնõȬ Ήçնƃ˷ žfմ̂ǫԲäӧαϯäɼαȾյ
VϑϋWϖ̔αΣP®¼ß®ƛdy mRNA  stress granule {z~̫ӵĢ|ԹՂjՊьҮΥʏ|l
fy_ϛwZQj^jPf~ӞŞɮψΫҺЎ~ĎЪ|uZwМΥѺx yzϟϹhwZ{ZQçˎPA. oryzae AoSO ´âÅ¤Ӕ®¼ß®ĬǾϋ|ԹǤǽ|šՀlfy^ 1)PAoSO ®¼ß®˿Ĕìx~Цј~ʁɉɿжʧ|ԫtwZƞњɿ_ю\QofxPòя~ԫӶ˘^|lfyϑϋyjw˷ϟϹҎ{tqQVˎ͜äЬ̈WA. oryzae |]d Saccharomyces cerevisiae Pab1p y Pub1p ~ÏÖà¥ AoPab1, AoPub1 stress granule ~Òå åyjwγZqQop EGFP yҌƣjw A. oryzae xψΫhnP®¼ß®˿Ĕìx~ҥȖҎ{tqQo~Ь̈PAoPab1-EGFP y AoPub1-EGFP ӳɉΥʏxЦјӔŒĢ|ǾǏjP®¼ß®˿ĔìxšՀjwՓЖΥ~̫ӵyfy_ҥȖhqQh|PAoPab1-mDsRed y AoSO-EGFP ~ŕψΫ̓ĥҚjPȥǏҧ̅Ҏ{tqyfPçԌxŕȥǏ_ҥȖhqQf~fy^PAoSO y stressgranule _ϓăĥγjwZfy_ϥƷhqQΫǏPAoSO _ stress granule ~ɦʒ|ԫîl^|uZwҺ
wZQ1) J. Maruyama et al. (2010) Biochem. Biophys. Res. Commun. Vol. 391, 868-873.
Localization Analysis of AoSO and Stress Granule in Aspergillus oryzae
Hsiang Ting HUANG, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO

85(Dept. of Biotechnol., Univ. of Tokyo)3,#,(%˴Ɛͱç·························································· 81"Շ˴ͱç ·························································· 43ՇȬ˵̀ ·························································· 68
ԯĳ˛ѭ ·························································· 71ѷʹӏù ·························································· 79˒ìϭҎ····················································· 31, 69Ⱦ҇ĦԉǼ······················································· 62Ǎ̻вш·························································· 67
øĳȴʄ·························································· 70
øĳɚù·························································· 18կδ˘Ř·························································· 65ĄƆȸǐ˒Ǽ ···················································· 41ϝą ˥ ·························································· 77ϝȺƮɌ ····················································· 30, 68ϝȶͨϿ ·························································· 45ϝδέш ·························································· 73͚ͤɠġ ·························································· 82
ϣ˼Ɏ͘ ·························································· 55Ė҇ѲԗǼ ······················································· 65Ė҇ Ȱ ····················································· 37, 46
Ė҇ՈǮ ·························································· 36϶ѿ ̞ ····················································· 27, 54
ąëŷǲǼ ······················································· 56
ő̲̉͠ ·························································· 83ȯìƲҘ ·························································· 42#ȟ͐Ѳ˘·························································· 41ȟ̳Ɯѓ·························································· 52ȟ̇ʐç·························································· 44բąОù·························································· 52$Ġę ǐ·························································· 48
ǢąƇĦǼ······················································· 76ǒƜ ̼·························································· 78ǒ˷Ҙç·························································· 28
̵˴ Ά·························································· 63ӬδСƟ·························································· 80
ĠƝϛш·························································· 49ĠR˴Ľʅ······················································· 78Ġ҇ϖù·························································· 38ĠԙƲО·························································· 62ĠԙŌ˝·························································· 72͏Ɛϒ̲ ·························································· 39ǫԵѫŘ ·························································· 12ǭδ˦Ǽ ····················································· 32, 80
ǫǞ ̼ ·························································· 71ȟȺϖɠ ·························································· 17ȟկѮƟ ····················································· 31, 81%ɻδ͢č·························································· 36u£! ·························································· 26ìȺѫɧ·························································· 74&ŷ͘ʥƴ ·························································· 75ΡȬήý ····················································· 27, 51
ŷ҇ϒ̲ ·························································· 75Ԥδ ǜ ·························································· 24ë˼˩č ·························································· 72þąҶù ·························································· 50̩ԙƖ՟ ····················································· 28, 58
Ⱥë Օ ·························································· 34ȺκЯɌ ·························································· 51

Ԝ˴ ˟·························································· 45
ğδƉý·························································· 82'Ʈ žˁ·························································· 47(!

ե̳ ɷ·························································· 39
δʖԶù·························································· 56
δôʍǼ·························································· 66

ѹԪԶɻ ·························································· 43˴ì ͨ ·························································· 3486δô ɩ ·························································· 76δôίɁ ·························································· 30δԈɠͅ ····················································· 35, 60

2̃ȣӓĊ·························································· 53̃δĪǫ·························································· 74õąͱç˱······················································· 59
f¬·························································· 15)ȝȶϖ̦ ·························································· 54

3*лȺҘѫ·························································· 48
ȌȶȄț·························································· 21ȗʕɻĳ ·························································· 464+˼Ɯ Ō·························································· 58Šδ͔ђǼ ······················································· 615,̢δԾç·························································· 84
̢˷ыǭ·························································· 66ô͕ǹĊ ·························································· 33ôӂƲý ·························································· 406-ȄδմƤԙյɒǼ ············································· 59Ϛѽǫӏ····················································· 26, 49
ȬŚŊӤ····················································· 33, 84
ȬȺ Ь····················································· 35, 60
Ȭȱӓ͘·························································· 40
Ȭղʍɡ·························································· 67ҝδ͢ɂ ·························································· 29ˍδшӏǼ ······················································· 70.
ѽƐǫϨ ·························································· 50̳˷ɑϨ ·························································· 57ѭδΛ˘ ·························································· 47ΏǑӿý ·························································· 73ƐŏĂԋ ·························································· 38ƐȴĲ˘ ·························································· 37ծ ͼǺ
857
Մ ϖɠ·························································· 69

8Ƥ̑Ĳϭ·························································· 63Ƥδ ы·························································· 77
ƤδϖΊ·························································· 61/̂ͧ˦ý ·························································· 79øδƌŌ ·························································· 44Ɍ˷ƶý ·························································· 53

90̇ Шĕ····················································· 32, 83ծ ͼǺ ...................................................................... 85ĂϪ͠ǘ ·························································· 42:1Ʋδ˯Ǽ·························································· 64͹ԇ ɩ·························································· 57
Ʋδձǭ····················································· 29, 64

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