Product Data - DIO SERIES DIETSThe “Original” High-Fat Diets forDiet Induced Obesity
Formulated by E. A. Ulman, Ph.D., Research Diets, Inc.,
8/26/98 and 3/11/99.

Research Diets, Inc. formulated the “original” high-fat diet for diet
induced obesity (DIO) studies in 1996. Today, our high-fat diets are
the research standard for DIO mice and rats worldwide.

DIO Low-Fat Control Diets
Matched, Purified Ingredient Diet
We recommend that you use a matched, purified ingredient dietand not a grain-based 'chow' diet. There are many, many differences
between purified diets and chow diets and these variables make it
difficult to interpret your data from a study in which one group
was fed a purified ingredient high-fat and the other a low-fat chow
diet. Differences between your groups could be due to the level offat, but could also be due to differences in fiber type and level,source of carbohydrate, and the presence or absence of plantchemicals (such as phytoestrogens), just to name a few.
See next page for low-fat control formulas.

Where NutriPhenomics Begins

ReportRepeatRevise(DIO) Formulas
Product #Protein
Carbohydrate
Fat
Total
kcal/gm

D12451
gm% kcal%
24
20
41
35
24
45
100
4.73

D12492
gm% kcal%
26
20
26
20
35
60
100
5.24

IngredientCasein, 30 Mesh
L-Cystine

gm
200
3

kcal
800
12

gm
200
3

kcal
800
12

Corn Starch
Maltodextrin 10
Sucrose

72.8
100
172.8

291
400
691

0
125
68.8

0
500
275

Cellulose, BW200

50

0

50

0

Soybean Oil
Lard

25
177.5

225
1598

25
245

225
2205

Mineral Mix S10026
DiCalcium Phosphate
Calcium Carbonate
Potassium Citrate, 1 H2O

10
13
5.5
16.5

0
0
0
0

10
13
5.5
16.5

0
0
0
0

Vitamin Mix V10001
Choline Bitartrate

10
2

40
0

10
2

40
0

FD&C Red Dye #40

0.05

0
0.05

0

773.854057

FD&C Blue Dye #1
Total

858.154057*Typical analysis of cholesterol inlard = 72 mg per 100 gram.
D12451 Cholesterol (mg)/4057 kcal = 167.8
Cholesterol (mg)/kg = 195.5
D12492 Cholesterol (mg)/4057 kcal = 216.4
Cholesterol (mg)/kg = 279.6
Research Diets, Inc.
20 Jules Lane
New Brunswick, NJ 08901 USA
Tel: 732.247.2390
Fax: 732.247.2340
info@researchdiets.com© 2011 Research Diets, Inc. All rights reserved. DIO-3000-2-13

Product Data - DIO SERIES DIETS
Low-Fat Control Formulas
There are many options for low-fat control diets.
Here are a few examples:
D12450B
Contains 35% sucrose by energy, unlike D12451 and D12492.
Sucrose is made up of glucose and fructose and it has been shownthat diets very high in sucrose or fructose (~60-70% by energy) can
induce hypertriglyceridemia, insulin resistance and fatty liver.

Match Sucrose Calories
Keep the amount of sucrose (as a percent of calories) constant across
low and high-fat diets. D12450H matches the sucrose calories inD12451. D12450J matches the sucrose calories in D12492.
These diets are formulated in such a way that when animals in the
low and high-fat groups consume the same number of calories, they
will also consume the same amount of sucrose.

Replace Sucrose with Corn Starch
D12450K contains no sucrose but only corn starch and maltodextrin
as the sources of carbohydrate. Maltodextrin is a partially hydrolyzed
form of corn starch that allows us to produce a quality pellet in a highcorn starch diet.

*Typical analysis of cholesterol inlard = 72 mg per 100 gram.
D12450B Cholesterol (mg)/4057 kcal = 54.4
Cholesterol (mg)/kg = 51.6

There are more possible low-fat diet formulas. Please contact one of
our scientists and we can help you decide if one of the above diets orperhaps a different, custom diet is better for your research.

35% Sucrose
Product #D12451 Match
17% Sucrose

D12492 Match
7% Sucrose

D12450H
kcal%
gm%
20.0
19.2
70.0
67.3
10.0
4.3
100.0
3.85

D12450J
kcal%
gm%
20.0
19.2
70.0
67.3
10.0
4.3
100.0
3.85

No Sucrose

Protein
Carbohydrate
Fat
Total
kcal/gm

D12450B
kcal%
gm%
20.0
19.2
67.3
70.0
10.0
4.3
100.0
3.85

IngredientCasein, 80 Mesh
L-Cystine

gm
200
3

kcal
800
12

gm
200
3

kcal
800
12

gm
200
3

kcal
800
12

gm
200
3

kcal
800
12

Corn Starch
Maltodextrin 10
Sucrose

315
35
350

1260
140
1400

452.2
75
172.8

1808.8
300
691.2

506.2
125
68.8

2024.8
500
275.2

550
150
0

2200
600
0

Cellulose, BW200

50

0

50

0

50

0

50

0

Soybean Oil
Lard

25
20

225
180

25
20

225
180

25
20

225
180

25
20

225
180

10
13
5.5
16.5

0
0
0
0

10
13
5.5
16.5

0
0
0
0

10
13
5.5
16.5

0
0
0
0

10
13
5.5
16.5

0
0
0
0

Vitamin Mix V10001
Choline Bitartrate

10
2

40
0

10
2

40
0

10
2

40
0

10
2

40
0

FD&C Yellow Dye #5
FD&C Red Dye #40
FD&C Blue Dye #1

0.05
0
0

0
0
0

0.04
0.01
0

0
0
0

0.04
0
0.01

0
0
0

0
0.025
0.025

0
0
0

1055.05

4057

1055.05

4057

1055.05

4057

1055.05

4057

Mineral Mix S10026
DiCalcium Phosphate
Calcium Carbonate
Potassium Citrate, 1 H2O

Total

D12450K
kcal%
gm%
20.0
19.2
70.0
67.3
10.0
4.3
100.0
3.85

D12451, D12492 The "Original High-Fat Diets"

D12451, D12492 The "Original High-Fat Diets"

These are just a sample of the hundreds of citations comprising the body of scientific work built
around these OpenSource diet formulas.

These are just a sample of the hundreds of citations comprising the body of scientific work built
around these OpenSource diet formulas.

References

References

DIO Rat Models
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7. Esler WP, Rudolph J, Claus TH, et al. Small-Molecule Ghrelin Receptor Antagonists
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DIO Mouse Models
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Ventricular Dysfunction in Nitric Oxide Synthase 3-Deficient Mice after Chronic
Pressure Overload. Journal of Nutrition. 2010;140(June):1438-1444.
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