RESEARCH PRODUCTS

Cambridge Isotope Laboratories, Inc.
isotope.com

SILAC Reagentsand Sets
Stable Isotope Labeling by
Amino Acids in Cell Culture
Stable isotope incorporation into protein has provento be a powerful technology for quantitatively comparing
the proteomes of multiple samples. Although isotopes can beincorporated metabolically or chemically, the SILAC method(utilizes metabolic incorporation) has emerged as one of themost powerful techniques for MS-based quantitativeapplications (see references on reverse).

Applications• Peptide/protein identification• Protein expression profiling (i.e., normal vs. disease cells)• Signaling pathway evaluation• Relative protein quantification

Cambridge Isotope Laboratories, Inc. (CIL) is pleased tooffer the following products for SILAC-based, quantitative
proteomic studies.

Amino Acids
L-Arginine (L-Arg)
Catalog No.

Description

CLM-2265-H*L-Arginine·HCl (13C6, 99%)

CNLM-539-H* L-Arginine·HCl (13C6, 99%; 15N4, 99%)
ULM-8347

L-Valine (L-Val)
Shift from Unlabeled

Catalog No.

Description+6 Da

CLM-2249-H*

L-Valine (13C5, 99%)

+5 Da+10 Da

DLM-488

L-Valine (D8, 98%)

+8 Da

N /A

ULM-8202

L-Valine (unlabeled)

N /A

L-Arginine·HCl (unlabeled)

Shift from Unlabeled*H denotes highly enriched amino acid (i.e., 99%).

L-Lysine (L-Lys)
Catalog No.

Description

CLM-2247-H*

L-Lysine·2HCl (13C6, 99%)

Shift from Unlabeled+6 Da

DLM-2640

L-Lysine·2HCl (4,4,5,5-D4, 96-98%)+4 Da

DLM-2641

L-Lysine·2HCl (3,3,4,4,5,5,6,6-D8, 98%)

+8 Da

CNLM-291-H* L-Lysine·2HCl (13C6, 99%; 15N2, 99%)

+8 Da

ULM-8766

N/A

L-Lysine·2HCl (unlabeled)Amino Acid Bundling Sets New!
SILAC 2-Plex (2-Plex SILAC Amino Acid Standards)†
Type

Abbreviated Compounds (Catalog Numbers)

Light (L)

Unlabeled L-Arg (ULM-8347) and Unlabeled L-Lys (ULM-8766)

Heavy (H)13

C6, 15N4 L-Arg (CNLM-539-H) and 13C6, 15N2 L-Lys (CNLM-291-H)

SILAC 3-Plex (3-Plex SILAC Amino Acid Standards)†

L-Leucine (L-Leu)
Catalog No.

Description

Shift from Unlabeled

CLM-2262-H*

L-Leucine (13C6, 99%)

+6 Da

DLM-1259

L-Leucine (5,5,5-D3, 99%)

+3 Da

CNLM-281-H* L-Leucine (13C6, 99%; 15N, 99%)

+7 Da

ULM-8203

N/A

L-Leucine (unlabeled)Type

Abbreviated Compounds (Catalog Numbers)

Light (L)

Unlabeled L-Arg (ULM-8347) and Unlabeled L-Lys (ULM-8766)Medium (M)

13Heavy (H)13

C6 L-Arg (CLM-2265-H) and D4 L-Lys (DLM-2640)
C6, 15N4 L-Arg (CNLM-539-H) and 13C6, 15N2 L-Lys (CNLM-291-H)

Each amino acid in the 2-plex and 3-plex standard sets is supplied in 0.1 g units.

†*H denotes highly enriched amino acid (i.e., 99%).

Visit isotope.com
for additionalamino acids.
Chemical purity (CP) is 98% or greater, unless otherwise indicated.
For research use only. Not for use in diagnostic procedures.

Please see other side for additional products of interest ➤Cambridge Isotope Laboratories, Inc.
North America: 1.800.322.1174 cilsales@isotope.com | International: +1.978.749.8000 intlsales@isotope.com | fax: 1.978.749.2768 | isotope.com

| SILAC Reagents

Cambridge Isotope Laboratories, Inc.

Other Products of Interest
Methionine (Met) Surrogates
L-Azidohomoalanine·HCl (light, AHA; heavy, hAHA) andL-azidonorleucine·HCl (ANL) can be used to evaluate the synthesis
and turnover of newly synthesized proteins in vivo through targetedor untargeted MS analysis (e.g., Yates JR et al. JPR 2015). CIL is
pleased to offer AHA, hAHA, and ANL for use in SILAC applications.
Please inquire for pricing.
Catalog No.

Description

CNLM-9461

L-Azidohomoalanine·HCl (1,2,3,4-13C4, 99%; 2,4-15N2, 98%)

ULM-9460

L-Azidohomoalanine·HCl (unlabeled)

ULM-10989

L-Azidonorleucine·HCl (unlabeled) CP 97%

Please visit isotope.com/applications ➝Proteomics ➝ Metabolic Labeling ➝ SILAC
for more information.

Chemical purity (CP) is 98% or greater, unless otherwise specified.

Example References
Mao, L.; Chen, J.; Lu, X.; et al. 2021. Proteomic analysis of lung cancer cells reveals
a critical role of BCAT1 in cancer cell metastasis. Theranostics, 11(19), 9705-9720.
Pino, L.K.; Baeza, J.; Lauman, R.; et al. 2021. Improved SILAC quantification with
data-independent acquisition to investigate bortezomib-induced protein degradation.
J Proteome Res, 20(4), 1918-1927.
Rathore, D.; Nita-Lazar, A. 2020. Phosphoproteome analysis in immune cell signaling.
Curr Protoc Immunol, 130(1), e105.
Bojkova, D.; Klann, K.; Koch, B.; et al. 2020. Proteomics of SARS-CoV-2-infected hostcells reveals therapy targets. Nature, 583(7816), 469-472.
Itzhak, D.N.; Sacco, F.; Nagaraj, N.; et al. 2019. SILAC-based quantitative proteomics
using mass spectrometry quantifies endoplasmic reticulum stress in whole HeLa cells.
Dis Model Mech, 12(11), dmm040741.
Shin, J.; Rhim, J.; Kown, Y.; et al. 2019. Comparative analysis of differentially secreted
proteins in serum-free and serum-containing media by using BONCAT and pulsed
SILAC. Sci Rep, 9(1), 3096.
Han, J.; Yi, S.; Zhao, X.; et al. 2019. Improved SILAC method for double labeling ofbacterial proteome. J Proteomics, 1(194), 89-98.
Duan, Q.; Li, D.; Xiong, L.; et al. 2019. SILAC quantitative proteomics and biochemical
analyses reveal a novel molecular mechanism by which ADAM12S promotes theproliferation, migration, and invasion of small cell lung cancer cells throughupregulating hexokinase 1. J Proteome Res, 18(7), 2903-2914.
McMillan, L.J.; Hwang, S.; Farah, R.E.; et al. 2018. Multiplex quantitative SILAC foranalysis of archaeal proteomes: a case study of oxidative stress responses. Environ
Microbiol, 20(1), 385-401.
Heo, S.; Diering, G.H.; Na, C.H.; et al. 2018. Identification of long-lived synaptic
proteins by proteomic analysis of synaptosome protein turnover. Proc Natl Acad Sci
U S A, 115(16), E3827-E3836.
Ma, Y.; McClatchy, D.B.; Barkallah, S.; et al. 2018. Quantitative analysis of newly
synthesized proteins. Nat Protoc, 13(8), 1744-1762.
Ma, Y.; McClatchy, D.B.; Barkallah, S.; et al. 2017. HILAQ: A novel strategy for newly
synthesized protein quantification. J Proteome Res, 16(6), 2213-2220.
Moody, L.R.; Barrett-Wilt, G.A.; Sussman, M.R.; et al. 2017. Glial fibrillary acidic protein
exhibits altered turnover kinetics in a mouse model of Alexander disease. J Biol Chem,292(14), 5814-5824.

Mondello, P.; Derenzini, E.; Asgari, Z.; et al. 2017. Dual inhibition of histone
deacetylases and phosphoinositide 3-kinase enhances therapeutic activity against
B cell lymphoma. Oncotarget, 8(8), 14017-14028.
Ji, Y.; Wei, S.; Hou, J.; et al. 2017. Integrated proteomic and N-glycoproteomic analysesof doxorubicin sensitive and resistant ovarian cancer cells reveal glycoprotein alterationin protein abundance and glycosylation. Oncotarget, 8(8), 13413-13427.
Diaz-Vera, J.; Palmer, S.; Hernandez-Fernaud, J.R.; et al. 2017. A proteomic approach
to identify endosomal cargoes controlling cancer invasiveness. J Cell Sci, 130(4),697-711.
Gonneaud, A.; Jones, C.; Turgeon, N.; et al. 2016. A SILAC-based method forquantitative proteomic analysis of intestinal organoids. Sci Rep, 6, 38195.
Gong, J.; Körner, R.; Gaitanos, L.; et al. 2016. Exosomes mediate cell contactindependent ephrin-Eph signaling during axon guidance. J Cell Biol, 214(1), 35-44.
McClatchy, D.B.; Ma, Y.; Liu, C.; et al. 2015. Pulsed azidohomoalanine labeling inmammals (PALM) detects changes in liver-specific LKB1 knockout mice. J Proteome Res,14(11), 4815-4822.
Bell-Temin, H.; Culver-Cochran, A.E.; Chaput, D.; et al. 2015. Novel molecular insights
into classical and alternative activation states of microglia as revealed by stable isotope
labeling by amino acids in cell culture (SILAC)-based proteomics. Mol Cell Proteomics,
14(12), 3173-3184.
Harel, M.; Oren-Giladi, P.; Kaidar-Person, O.; et al. 2015. Proteomics of microparticles
with SILAC quantification (PROMIS-Quan): a novel proteomic method for plasma
biomarker quantification. Mol Cell Proteomics, 14(4), 1127-1136.
Edfors, F.; Boström, T.; Forsström, B.; et al. 2014. Immunoproteomics using polyclonal
antibodies and stable isotope-labeled affinity-purified recombinant proteins. Mol CellProteomics, 13(6), 1611-1624.
Bagert, J.D.; Xie, Y.J.; Sweredoski, M.J.; et al. 2014. Quantitative, time-resolved
proteomic analysis by combining bioorthogonal noncanonical amino acid tagging andpulsed stable isotope labeling by amino acids in cell culture. Mol Cell Proteomics,
13(5), 1352-1358.
Ong, S.E.; Mann, M. 2006. A practical recipe for stable isotope labeling by amino acidsin cell culture (SILAC). Nat Protoc, 1(6), 2650-2660.

Luo, Y.; Mok, T.S.; Lin, X.; et al. 2017. SWATH-based proteomics identified carbonic
anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma.
Sci Rep, 7, 41191.

Cambridge Isotope Laboratories, Inc. 3 Highwood Drive, Tewksbury, MA 01876 USA
North America: 1.800.322.1174 | International: +1.978.749.8000 | fax: +1.978.749.2768 | isotope.com

PRT_SILAC (8/5/22)
Supersedes all previously published literature