Stable Isotope labeled RNA and DNA Building Blocks:
rNTPs and dNTPs
P1: Stable Isotope Labeled rNTPs and dNTPs
NMRによる分子量の大きなDNAとRNA構造解明には、これら分子が安定同位体に標識（ラベル化）される必要があります。
シランテス社（Silantes）は幅広い安定同位体ラベル化したリボ、デオキシリボヌクレオシド三リン酸の様々な核種の組み合わせ2H、13C、15N、19Fと18Oを提供しています。
ラベル化されたrNTPとdNTPの全製品リストは、次の添付した表をご確認ください。

Silantes Technology
シランテス社の安定同位体ラベルのヌクレオチドは、バクテリア由来のDNAとRNAから製造されます。バクテリア株が有機物H2、O2とCO2上で増殖され、化学的に得られます。抽出されたDNAまたはRNAは酵素によって加水分解されます。単離された5’-NMP は酵素でリン酸化された 5’-NDP と5’-NTP となり、IC-およびRP-HPLCによって精製されます。

図1: シランテス社のバイオテクノロジーによるNTP製造プロセス

安定同位体を用いたバイオマスのin vivo 濃縮技術により、以下の結果が得られます。

- ラベル化されたrNTPとdNTPの費用対効果として、安定同位体の効率的な導入- クローズドシステムを使用することによるヌクレオチドの効率的で均一な安定同位体ラベル（>98atom%）High Quality Commitment
シランテス社は、HPLCによって決定された同位体純度>98atom%と化学純度>95%を保証します。図2はrCTPのHPLC溶出データの例を示しています。
さらに、生物学的能力、例えば酵素オリゴ合成のrNTPとdNTPの適合性は、それぞれin vivoでのRNAおよびDNAの合成によって検証されています。

図2: rCTPのRP-HPLC溶出データ

Appendix: Product Lists
rNTPs and dNTPs
Appendix I: Product List of
Stable Isotope-Labeled and fluorinated rNTPs and dNTPs
Article #DescriptionIsotope

Unit

Nucleoside-Triphosphates (NTPs)

120103601
120103602
120103603
120103604
120103801
120103802
120103803
120103804
120106100
120106200

Deoxy-Nucleoside-Triphosphates 2H-labeled
Deoxyadenosine 5'-triphosphate, lithium salt, solution
Deoxycytidine 5'-triphosphate, lithium salt, solution
Deoxyguanosine 5'-triphosphate, lithium salt, solution
Thymidine 5'-triphosphate, lithium salt, solution
Deoxyadenosine 5'-triphosphate, lithium salt, solution
Deoxycytidine 5'-triphosphate, lithium salt, solution
Deoxyguanosine 5'-triphosphate, lithium salt, solution
Thymidine 5'-triphosphate, lithium salt, solution
Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution
Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution

2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)

10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg120203601
120203602
120203603
120203604
120203801
120203802
120203803
120203804
120206100
120206200

Deoxy-Nucleoside-Triphosphates 13C-labeled
Deoxyadenosine 5'-triphosphate, lithium salt, solution
Deoxycytidine 5'-triphosphate, lithium salt, solution
Deoxyguanosine 5'-triphosphate, lithium salt, solution
Thymidine 5'-triphosphate, lithium salt, solution
Deoxyadenosine 5'-triphosphate, lithium salt, solution
Deoxycytidine 5'-triphosphate, lithium salt, solution
Deoxyguanosine 5'-triphosphate, lithium salt, solution
Thymidine 5'-triphosphate, lithium salt, solution
Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution
Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution

13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)

10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg120303601
120303602
120303603
120303604
120303801
120303802
120303803
120303804
120306100
120306200

Deoxy-Nucleoside-Triphosphates 15N-labeled
Deoxyadenosine 5'-triphosphate lithium salt, solution
Deoxycytidine 5'-triphosphate, lithium salt, solution
Deoxyguanosine 5'-triphosphate, lithium salt, solution
Thymidine 5'-triphosphate lithium salt, solution
Deoxyadenosine 5'-triphosphate lithium salt, solutionDeoxycytidine 5'-triphosphate, lithium salt, solution
Deoxyguanosine 5'-triphosphate, lithium salt, solution
Thymidine 5'-triphosphate lithium salt, solution
Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution
Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution

15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)

10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)

10 mg

Deoxy-Nucleoside-Triphosphates 2H15N-labeled120503601 Deoxyadenosine 5'-triphosphate, lithium salt, solution
120503602 Deoxycytidine 5'-triphosphate, lithium salt, solution
120503603 Deoxyguanosine 5'-triphosphate, lithium salt, solution
120503604 Thymidine 5'-triphosphate, lithium salt, solution

10 mg
10 mg
10 mg

Appendix: Product Lists
rNTPs and dNTPs
120503801 Deoxyadenosine 5'-triphosphate, lithium salt, solution
120503802 Deoxycytidine 5'-triphosphate, lithium salt, solution
120503803 Deoxyguanosine 5'-triphosphate, lithium salt, solution
120503804 Thymidine 5'-triphosphate, lithium salt, solution
120506100 Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution
120506200 Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution

Deoxy-Nucleoside-Triphosphates 13C15N-labeled120603601 Deoxyadenosine 5'-triphosphate, lithium salt, solution
120603602 Deoxycytidine 5'-triphosphate, lithium salt, solution
120603603 Deoxyguanosine 5'-triphosphate, lithium salt, solution
120603604 Thymidine 5'-triphosphate lithium salt, solution
120603801 Deoxyadenosine 5'-triphosphate, lithium salt, solution
120603802 Deoxycytidine 5'-triphosphate, lithium salt, solution
120603803 Deoxyguanosine 5'-triphosphate, lithium salt, solution
120603804 Thymidine 5'-triphosphate, lithium salt, solution
120606100 Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution
120606200 Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution

Deoxy-Nucleoside-Triphosphates 2H13C15N-labeled120703601 Deoxyadenosine 5'-triphosphate, lithium salt, solution
120703602 Deoxycytidine 5'-triphosphate, lithium salt, solution
120703603 Deoxyguanosine 5'-triphosphate, lithium salt, solution
120703604 Thymidine 5'-triphosphate, lithium salt, solution
120703801 Deoxyadenosine 5'-triphosphate, lithium salt, solution
120703802 Deoxycytidine 5'-triphosphate, lithium salt, solution
120703803 Deoxyguanosine 5'-triphosphate, lithium salt, solution
120703804 Thymidine 5'-triphosphate, lithium salt, solution
120706100 Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution

2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)

50 mg

13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)

10 mg

2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)

10 mg

50 mg
50 mg
50 mg
4x10mg
4x50mg

10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg

10 mg
10 mg
10 mg
50 mg
50 mg
50 mg50 mg
4x10mgAppendix: Product Lists
rNTPs and dNTPs
120706200 Set of 4 deoxyribonucleoside 5'-triphosphates, lithium salt, solution

121103601
121103602
121103603
121103604
121103801
121103802
121103803
121103804
121106100
121106200

Ribo-Nucleoside-Triphosphates 2H-labeled
Adenosine 5'-triphosphate, lithium salt, solution
Cytidine 5'-triphosphate, lithium salt, solution
Guanosine 5'-triphosphate, lithium salt, solution
Uridine 5'-triphosphate, lithium salt, solution
Adenosine 5'-triphosphate, lithium salt, solution
Cytidine 5'-triphosphate, lithium salt, solution
Guanosine 5'-triphosphate, lithium salt, solution
Uridine 5'-triphosphate, lithium salt, solution
Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution
Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution

121203601
121203602
121203603
121203604
121203801
121203802
121203803
121203804
121206100
121206200
121213601

Ribo-Nucleoside-Triphosphates 13C-labeled
Adenosine 5'-triphosphate, lithium salt, solution
Cytidine 5'-triphosphate, lithium salt, solution
Guanosine 5'-triphosphate, lithium salt, solution
Uridine 5'-triphosphate, lithium salt, solution
Adenosine 5'-triphosphate, lithium salt, solution
Cytidine 5'-triphosphate, lithium salt, solution
Guanosine 5'-triphosphate, lithium salt, solution
Uridine 5'-triphosphate, lithium salt, solution
Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution
Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution
Adenosine 5'-triphosphate (site-specifically 13C8-labeled), lithium salt, solution

121213603

Guanosine 5'-triphosphate (site-specifically 13C8-labeled), lithium salt, solution

121213801

Adenosine 5'-triphosphate (site-specifically 13C8-labeled), lithium salt, solution

121213803

Guanosine 5'-triphosphate (site-specifically 13C8-labeled), lithium salt, solution

121303601
121303602
121303603
121303604
121303605
121303801
121303802
121303803
121303804
121303805
121306100
121306200

Ribo-Nucleoside-Triphosphates 15N-labeled
Adenosine 5'-triphosphate lithium salt, solution
Cytidine 5'-triphosphate, lithium salt, solution
Guanosine 5'-triphosphate, lithium salt, solution
Uridine 5'-triphosphate, lithium salt, solution
Inosine 5'-triphosphate, lithium salt, solution
Adenosine 5'-triphosphate lithium salt, solution
Cytidine 5'-triphosphate, lithium salt, solution
Guanosine 5'-triphosphate, lithium salt, solution
Uridine 5'-triphosphate, lithium salt, solution
Inosine 5'-triphosphate, lithium salt, solution
Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution
Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution

121413602
121413604

2H,13C,15N(Uniform)

4x50mg2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)
2H (Uniform)

10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg

13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Uniform)
13C (Sitespecific)
13C (Sitespecific)
13C (Sitespecific)
13C (Sitespecific)

10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg
10 mg

15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)
15N (Uniform)

10 mg
10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
50 mg4x10mg
4x50mgRibo-Nucleoside-Triphosphates 2H13C-labeled
Cytidine 5'-triphosphate (site-specifically 2H5 13C6-labeled), lithium salt, solution 2H,13C (Sitespecific)
Uridine 5'-triphosphate (site-specifically2H5,13C6-labeled), lithium salt, solution
2H,13C (Sitespecific)

10 mg
50 mg
50 mg

10 mg
10 mg

Appendix: Product Lists
rNTPs and dNTPs
121413802
121413804

121503601

Cytidine 5'-triphosphate (site-specifically 2H5 13C6-labeled), lithium salt, solution 2H,13C (Sitespecific)
Uridine 5'-triphosphate (site-specifically 2H5,13C6-labeled), lithium salt, solution 2H,13C (Sitespecific)
Ribo-Nucleoside-Triphosphates 2H15N-labeled
Adenosine 5'-triphosphate, lithium salt, solution

121503602 Cytidine 5'-triphosphate, lithium salt, solution
121503603 Guanosine 5'-triphosphate, lithium salt, solution
121503604 Uridine 5'-triphosphate, lithium salt, solution
121503801

Adenosine 5'-triphosphate, lithium salt, solution

121503802 Cytidine 5'-triphosphate, lithium salt, solution
121503803 Guanosine 5'-triphosphate, lithium salt, solution
121503804 Uridine 5'-triphosphate, lithium salt, solution
121506100

Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution

121506200 Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution

121603601

Ribo-Nucleoside-Triphosphates 13C15N-labeled
Adenosine 5'-triphosphate, lithium salt, solution

121603602 Cytidine 5'-triphosphate, lithium salt, solution
121603603 Guanosine 5'-triphosphate, lithium salt, solution
121603604 Uridine 5'-triphosphate, lithium salt, solution
121603605 Inosine 5'-triphosphate, lithium salt, solution
121603801

Adenosine 5'-triphosphate, lithium salt, solution

121603802 Cytidine 5'-triphosphate, lithium salt, solution
121603803 Guanosine 5'-triphosphate, lithium salt, solution
121603804 Uridine 5'-triphosphate, lithium salt, solution
121603805 Inosine 5'-triphosphate, lithium salt, solution
121606100

Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution

121606200 Set of 4 ribonucleoside 5'-triphosphates, lithium salt, solution

Ribo-Nucleoside-Triphosphates 2H13C15N-labeled

50 mg
50 mg

2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)
2H,15N(Uniform)10 mg

13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)
13C,15N(Uniform)

10 mg

10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg

10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mgAppendix: Product Lists
rNTPs and dNTPs
121703601

Adenosine 5'-triphosphate, lithium salt, solution

121703602 Cytidine 5'-triphosphate, lithium salt, solution
121703603 Guanosine 5'-triphosphate, lithium salt, solution
121703604 Uridine 5'-triphosphate, lithium salt, solution
121703801

Adenosine 5'-triphosphate, lithium salt, solution

121703802 Cytidine 5'-triphosphate, lithium salt, solution
121703803 Guanosine 5'-triphosphate, lithium salt, solution
121703804 Uridine 5'-triphosphate, lithium salt, solution
121706100

Set of 4 ribonucleside 5'-triphosphates, solution

121706200 Set of 4 ribonucleoside 5'-triphosphates, solution

2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)
2H,13C,15N(Uniform)

10 mg
10 mg
10 mg
10 mg
50 mg
50 mg
50 mg
50 mg
4x10mg
4x50mg