User Guide
Quick-Muscle™ Skeletal - Maintenance Medium
Catalog Number: SM-MMIntroduction
Quick-Muscle™ Skeletal - Maintenance Medium may be used for the long-term maintenance of human pluripotent stem
cell-derived skeletal muscle cells following differentiation as outlined in the Quick-Muscle™ Skeletal - SeV Kit user guides.
Quick-Muscle™ Skeletal differentiated cell cultures display typical skeletal muscle morphology and markers, such as
myosin heavy chain (MHC). When handled and maintained according to the instructions in this user guide, skeletal musclecells are viable long-term.
Scale:The Quick-Muscle™ Skeletal - Maintenance Medium provides sufficient medium for 4 wells of a24-well plate, 1 well of a 6-well plate, or 16 wells of a 96-well plate for up to 2 weeks.

Related Products:

Quick-Muscle™ Skeletal - SeV Kit, Catalog Number: SM-SeVContents
Upon receipt, store the reagents at the temperatures indicated in the table below. All reagents are shipped on dry ice.
Contents

VolumeStorage

Thaw

Component M1

750 μl-20°C or -80°COn ice or 4°C

Component P

50 μl-20°C or -80°C

Room temperature

Condition of Use
This product is for research use only. It is not approved for use in humans or for therapeutic or diagnostic use.

Technical Support
For technical support please refer to the FAQ on our website.
You may also contact us at cs@elixirgensci.com or call +1 (443) 869-5420 (M-F 9am-5pm EST).

Last revised: July 14, 2023

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1Required Consumables
ItemVendor

Catalog Number

DMEM/F12

ThermoFisher

21331020

GlutaMAX

ThermoFisher

35050061

Penicillin-Streptomycin

ThermoFisher

15140122

Preparation
Medium M11.

Prepare Medium M1 using the reagents listed in the table below.
○ Thaw Component M1 for 20-30 minutes at the temperature indicated in the “Contents” table on page 1.
○ Warm all other reagents at room temperature for 20-30 minutes.
○ Tap the Component M1 tube 3 times and then briefly spin it down before use.
○ Keep Medium M1, and any subsequent media made with it, protected from light.
○ Store Medium M1 for up to 2 weeks at 4°C.
○ Leftover Component M1 can be discarded or saved at 4°C for up to two weeks.
Reagents

VolumeDMEM/F1214 ml

GlutaMAX

147.6 μl

Penicillin-Streptomycin (10000 units/ml; 100x)147.6 μl

Component M1

443 μlFirst Week
1.

Prepare Medium M1(P) using the reagents listed in the table below.
○ Thaw Component P for 20-30 minutes at the temperature indicated in the “Contents” table on page 1.
○ Warm all other reagents at room temperature for 20-30 minutes.
○ Tap the Component P tube 3 times and then briefly spin it down before use.
○ Store Medium M1(P) for up to 2 weeks at 4°C.
○ Leftover Component P can be saved at 4°C.
Reagents

VolumeMedium M1

7 ml

Component P

3.5 μl

2. Pipet out most of the old medium, but not completely (i.e., just enough to cover the surface of the well), from eachwell and very slowly along the wall of the well, add Medium M1(P) according to the following table.

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2Required volume per well
Reagents
Medium M1(P)6-well plate24-well plate

96-well plate

2 ml

800 µl150 µl3. Incubate the cultures at 37°C, 5% CO2 for 2 days.
4. For subsequent medium changes, pipet out half (see volumes in the table above) of the old medium from each welland replace with an equal volume of room temperature Medium M1(P).
5. Repeat Step 4 every 2-3 days such as on Monday, Wednesday, and Friday for 1 week.

Second Week
1. Warm Medium M1 at room temperature for 20-30 minutes until it no longer feels cold.
2. Pipet out most of the old medium, but not completely (i.e., just enough to cover the surface of the well), from eachwell and very slowly along the wall of the well, add Medium M1 according to the following table.
Required volume per wellReagents

6-well plate24-well plate

96-well plate

Medium M12 ml

800 µl150 µl3. Incubate the cultures at 37°C, 5% CO2 for 2 days.
4. For subsequent medium changes, pipet out half (see volumes in the table above) of the old medium from each welland replace with an equal volume of room temperature Medium M1.
5. Repeat Step 4 every 2-3 days such as on Monday, Wednesday, and Friday for 1 week.

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