RESEARCH PRODUCTS

Cambridge Isotope Laboratories, Inc.
isotope.com

Metabolite and Lipid
Yeast Extracts
For MS Metabolomics and Lipidomics

Cambridge Isotope Laboratories, Inc. (CIL) is pleased to offer 13C-labeled and unlabeled yeast extracts for use in MS-based
quantitative or profiling studies of various sample types. These extracts have been rigorously characterized by a number ofmethodologies and are amenable to a variety of research uses after simple reconstitution. The components in the extracts span broad
metabolic classes (e.g., amino and organic acids, sugar phosphates, coenzymes, fatty acids and lipids), biochemical pathways (e.g., citrate
and glyoxylate cycle, nucleotide and lipid metabolism), and cellular/molecular processes (e.g., intracellular signaling, immune system, blood
coagulation, lipolysis).

Overview
Catalog No.
ISO1
ISO1-UNL
NEW! ISO1-KIT
NEW! L-ISO1
NEW! L-ISO1-UNL

Uses

Description
Metabolite Yeast Extract (U-13C, 98%)
Metabolite Yeast Extract (unlabeled)
Metabolite Yeast Extract Kit
Crude Lipid Yeast Extract (U-13C, 99%)
Crude Lipid Yeast Extract (unlabeled)•	Targeted or untargeted, MS-based analysis• Method and instrument QC• Quantitation• Biomarker discovery and verification

Benefits

Dry extract of Pichia pastoris cells (strain CBS 7435).
Produced by ISOtopic Solutions (isotopic-solutions.com).
Please inquirefor pricing.

•	Reduces measurement uncertainty• Improves precision and accuracy• Enhances identification confidence• Decreases development time and cost

Example References
Reiter, A.; Asgari, J.; Wiechert, W.; et al. 2022. Metabolic footprinting of microbial systems
based on comprehensive in silico predictions of MS/MS relevant data. Metabolites, 12(3),257-280.
Zhao, X.; Golic, F.T.; Harrison, B.R,; et al. 2022. The metabolome as a biomarker of aging
in Drosophila melanogaster. Aging Cell, 21(2), e13548.

Guijas, C.; Montenegro-Burke, J.R.; Domingo-Almenara, X.; et al. 2018. METLIN: A technology
platform for identifying knowns and unknowns. Anal Chem, 90(5), 3156-3164.
Si-Hung, L.; Causon, T.J.; Hann, S. 2017. Comparison of fully wettable RPLC stationary phases
for LC-MS-based cellular metabolomics. Electrophoresis, 38(18), 2287-2295.

Rampler, E.; Hermann, G.; Grabmann, G.; et al. 2021. Benchmarking non-targetedmetabolomics using yeast-derived libraries. Metabolites, 11(3), 160-179.

Schwaiger, M.; Rampler, E.; Hermann, G.; et al. 2017. Anion-exchange chromatography
coupled to high-resolution mass spectrometry: A powerful tool for merging targeted andnon-targeted metabolomics. Anal Chem, 89(14), 7667-7674.

Mairinger, T.; Weiner, T.; Hann, S.; et al. 2020. Selective and accurate quantification ofN-acetylglucosamine in biotechnological cell samples via GC-MS/MS and GC-TOFMS.
Anal Chem, 92(7), 4875-4883.

Ortmayr, K.; Hann, S.; Koellensperger, G. 2015. Complementing reversed-phase selectivity
with porous graphitized carbon to increase the metabolome coverage in an on-line
two-dimensional LC-MS setup for metabolomics. Analyst, 140(10), 3465-3473.

Galvez, L.; Rusz, M.; Schwaiger-Haber, M.; et al. 2019. Preclinical studies on metal based
anticancer drugs as enabled by integrated metallomics and metabolomics. Metallomics,11(10), 1716-1728.

Neubauer, S.; Chu, D.B.; Marx, H.; et al. 2015. LC-MS/MS-based analysis of coenzyme A
and short-chain acyl-coenzyme A thioesters. Anal Bioanal Chem, 407(22), 6681-6688.

Demarest, T.G.; Truong, G.T.D.; Lovett, J.; et al. 2019. Assessment of NAD+ metabolism
in human cell cultures, erythrocytes, cerebrospinal fluid and primate skeletal muscle.
Anal Biochem, 572, 1-8.

Application NotePercy, A.J.; Munjoma, N.; Heywood, D.; et al. 2022. Targeted MRM screening of U-13C lipidyeast extracts for robust lipidomics applications (CIL application note #54).

 ermann, G.; Schwaiger, M.; Volejnik, P.; et al. 2018. 13C-labelled yeast as internal standard
Hfor LC-MS/MS and LC high resolution MS-based amino acid quantification in human plasma.
J Pharm Biomed Anal, 155, 329-334.

Cambridge Isotope Laboratories, Inc.
North America: 1.800.322.1174 cilsales@isotope.com | International: +1.978.749.8000 intlsales@isotope.com | fax: 1.978.749.2768 | isotope.com
MET_RSCH_YEAST (10/13/22)
Supersedes all previously published literature