ChemiDoc™ Go Imaging Systemwith Image Lab Touch Software
Version 4.0
User Guide

ChemiDoc Go Imaging System with
Image Lab Touch Software
User Guide
Version 4.0

Technical Support Department
The Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific time.
Phone: 1-800-424-6723, option 2
Email: Support@bio-rad.com.com (U.S./Canada Only)
For technical assistance outside the U.S. and Canada, contact your local technical support office or click the Contact Us linkat www.bio-rad.com.

Legal Notices
No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical,
including photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad
Laboratories, Inc.
Bio-Rad reserves the right to modify its products and services at any time. This guide is subject to change without notice.
Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors or omissions, or for any damage resulting
from the application or use of this information.
The ChemiDoc Go uses the Linux operating system, which contains software licensed under the following licenses as wellas others:n GNu Public License v 1.0, 2.0, 3.0
n Lesser GNu Public License v 2.0, 2.1, 3.0
n Apache License v 2.0
n BSD License
Image Lab Touch is based in part on the work of the CImg project (http://cimg.eu/). To see the license for details, paste thefollowing link into a web browser:
http://cecill.info/licences/Licence_CeCILL-C_V1-en.txt
The software provided as part of the ChemiDoc Go includes free open source and public-source software. Certain OPEN
LICENSES require that the source materials be made available to recipients or other requestors under the terms of thesame OPEN LICENSE. The corresponding open source software can be downloaded at:
http://bio-rad.com/ChemiDocGo-opensourceTo view the OPEN LICENSES
1. Open the Online Help system on your Imaging System.
2. Tap Help Topics at the bottom of the screen, and then tap Legal Notices.
3. Insert a USB drive into the port on your instrument.
4. Tap the Download Software Licenses button located in the bottom-right corner of the screen. The license information
is downloaded to a Software Licenses folder created on the USB drive.
SYBR is a trademark of Thermo Fisher Scientific Inc.
All trademarks used herein are the property of their respective owner.
Copyright ©2024 by Bio-Rad Laboratories, Inc. All rights reserved.

Revision History
Document
ChemiDoc Go Imaging Systemwith Image Lab Touch Software(Doc ID# 10000167057)

Date

Description of Change

March 2024

First ReleaseTable of Contents
ChemiDocGoUG_TitlePage
Revision History

Chapter 1 Safety and Regulatory Compliance

1
vii

15

Warranty

15

General Precautions

16

Regulatory Notices

17

Safety Alerts

17

Types of Safety Hazards

17

Levels of Potential Risk

18

Instrument Safety Warnings

19

Instrument Safety Cautions

20

Excising Bands from Samples

20

Environmental and Safe Use Requirements

21

Safety and Regulatory Compliance

22

Safety Compliance

22

Electromagnetic Compatibility (EMC)22

EMC Warnings and Notes

23

Notice24

Power Safety

24

Fuses

24

Chapter 2 Introduction

25

Product Features

25

Front and Side Panel Components

27

Rear Panel Components

28

CMOS Camera

29

Image Lab Touch Software

29

Emission Filters

29

Optional Accessories

30

Instrument Guide | ix

Table of Contents

Sample Trays
Technical Specifications

Chapter 3 Starting Image Lab Touch

30
31

33

About the Welcome Screen

33

What's in the Suitcase?

34

Interacting with the Touch Screen

34Entering Text on the Touch Screen

35

Using the On-Screen Keyboard

35

Using the On-Screen Keypad37

Choosing Imaging Settings

38

Locking the System
Unlocking the System
Getting Help

Chapter 4 Logging In to Image Lab Touch
Creating the First User Account

40
41
42

43
43

Creating a Password

44

Changing a Password

45

Removing a Password

47

Linking to Your Bio-Rad BR.io Account48

Creating the First Administrator Account52

Becoming an Administrator after Initial Login53

Setting Administrator Options

54

Enabling the Diagnostic Port on the ChemiDoc Go Imaging System

57

Restricting Access to BR.io

58

Chapter 5 Understanding the Views

61

Main Toolbar Settings

61

Camera View

63

Preview

65

Image View

66

The Image View Toolbar

67

The Gallery

68

Color Cues

70

x | ChemiDoc Go Imaging System with Image Lab Touch Software

Table of Contents

Chapter 6 Choosing a Sample Tray

73

Applications Listed by Tray Type75

UV/Stain-Free Tray

75

Blue Tray

75

White Tray

76

Chapter 7 Acquiring Images

79

Placing the Sample in the Imager

80

Acquiring an Image — General Steps

80

Setting Image Type and Size82

Choosing Image Type82

Choosing Image Size82

Choosing the Application

84

Single or Multichannel Images

86(Multichannel) Selecting a Channel Color

86

Previewing an Image

87

Exposure Settings

89

Setting Exposure for Most Gels and Blots

89

Setting Exposure for Stain-Free Gels

90

Setting Exposure Time for a Chemiluminescent Blot

90

Automatic Exposure Settings

91

Setting the Exposure Automatically for Chemiluminescent Applications

91

Specifying the Region of Interest for Auto-Exposure

92

Setting the Exposure Manually

93(Single Channel) Acquiring Signal Accumulation Mode (SAM) Images
Adjusting SAM Acquisition Settings
Acquiring the Image

95
97
98

(Stain-Free Gel) Skipping Gel Activation

99(Stain-Free Gel) Acquiring an Image after Canceling Acquisition

99(Multichannel) Retaking an Image

99

Chapter 8 Excising Bands from Samples

101

Excising Bands on a UV/Stain-Free Tray

101

Excising Bands on a White Tray

106

Excising Bands on a Blue Tray

107

Instrument Guide | xi

Table of Contents

Chapter 9 Viewing Images

109

Accessing the Gallery

109

Searching for Images in the Gallery

110

Selecting and Opening Images in the Gallery

111

Sorting Images in the Gallery by Date112

Checking Intensity Values

113

Viewing Multichannel Images in Image View

115

Viewing a Composite or Merged Image in Grayscale

117

Comparing Images

119

Copying Image Settings

121

Viewing Image Information

122

Viewing SAM Images

123

Chapter 10 Merging Images

125

Naming Merged Images

127

Chapter 11 Managing Images

129

Adding Notes to an Image File129

Changing the File Naming Format

130

Renaming Images

132

Renaming SAM Images

133

Printing Images

134

Deleting Images

134

Deleting SAM Images

135

Chapter 12 Exporting Images

137

Exporting Images

138

Export Formats

141

Choosing Export Formats

141

Exporting to BR.io

143

Exporting to a USB Drive or External Hard Drive

144

Preparing to Export Images to a Shared Folder

146

Creating a Shared Folder on a Windows Computer

146

Creating a Shared Folder on a Mac Computer

149

Determining the UNC of a Previously Shared Folder

152

Determining the UNC Path to a Network Folder on a Windows Computerxii | ChemiDoc Go Imaging System with Image Lab Touch Software

152

Table of Contents

Determining the UNC Path to a Network Folder on a Mac Computer

153

Exporting to a Shared Folder for the First Time

155

Exporting to a Shared Folder on a Network or Computer

156

Entering Log-in Credentials

156

Exporting to a Recent Location

158

Removing Network Locations

160

Disconnecting from a Shared Folder

Chapter 13 System Settings

160

161

Setting the System Date, Time, and Time Zone

161

Setting the Sound Volume

164

Setting the System Language

164

Configuring the Network Connection

165

Appendix A Maintaining the Imaging System
Updating the Software and Firmware on ChemiDoc Go Imagers

169
169

Verifying the Current Software and Firmware Versions

170

Updating the Software and Firmware via the Internet

170

Updating the Software and Firmware via USB Drive

172

Ensuring the Instrument is Level173

To level the instrument

173

Cleaning the Instrument

174

Maintaining the LED Lamps

175

Replacing the Fuses

175

Appendix B Important Imaging Concepts

179

Exposure Settings for Chemiluminescent Applications

179

Specifying the Region of Interest for Optimal Exposure

179

Adjusting How Images Are Displayed

180

Adjusting Image Brightness and Contrast

180

Restoring Default Settings

182

The Image Resolution/Time Scale183

How Binning Settings Affect Image Quality

184(Single Channel) Signal Accumulation Mode

186

Instrument Guide | xiii

Table of Contents

Appendix C Free and Open-Source Notices for ChemiDoc Go Imaging Systems

187

Appendix D Ordering Information

189

ChemiDocGoUG_SigPage

193

xiv | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 1 Safety and Regulatory Compliance
Note: This instrument has been tested and found to comply with the limits for a Class A digital device
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
instrument generates, uses, and can radiate radiofrequency energy. If not installed and used inaccordance with the instruction manual, it may cause harmful interference to radio communications.
Operation of this instrument is intended for laboratory use only and if used in a residential area, islikely to cause harmful interference in which case users will be required to correct the interference attheir own expense.
WARNING: Because some imaging applications involve UV illumination, the Bio-Rad
imaging systems are labeled as Group 3 (High Risk) by the International ElectrotechnicalCommision standard IEC/EN 62471. The imaging systems should be used only by trained
personnel who know the health risks associated with the UV radiation normally associatedwith these instruments. Users should be trained on the appropriate personal protective
equipment for working with UV light to minimize UV exposure.

Warranty
The ChemiDoc Go is warrantied against defects in materials and workmanship for one year. If any defectoccurs in the instrument during this warranty period, Bio-Rad Laboratories, Inc. will repair or replace thedefective parts at its discretion without charge. The following defects, however, are specifically excluded:nDefects caused by improper operationn

Repair or modification done by anyone other than Bio-Rad Laboratories, Inc. or the company’s
authorized agentn

Use of spare parts supplied by anyone other than Bio-Rad Laboratories, Inc.

n

Damage caused by accident or misusenDamage caused by disasternCorrosion caused by improper solvents or samples

Instrument Guide | 15

Chapter 1 Safety and Regulatory Compliance

General PrecautionsnRead the user guide carefully.

n

Use the instrument only for the intended purpose of Stain-Free, gel, and blot image acquisition.

nConnect the instrument to a grounded power source and to a circuit breaker.

n

Do not pour liquids on or inside the instrument.

nClean the sample tray after use.

16 | ChemiDoc Go Imaging System with Image Lab Touch Software

Regulatory Notices

Regulatory Notices
The ChemiDoc Go is designed and certified to meet EN 61010, the internationally accepted electricalsafety standard, EMC regulations, and TUV requirements. Certified products are safe to use when
operated in accordance with this user guide. Do not modify or alter these instruments in any way.
Modification or alteration of these instruments willn

Void the manufacturer’s warrantynVoid the regulatory certificationsnCreate a potential safety hazard
Bio-Rad Laboratories, Inc. is not responsible for any injury or damage caused by use of
these instruments for purposes other than those for which they are intended, or bymodifications of the instruments not performed by Bio-Rad Laboratories, Inc. or an
authorized agent.

Safety Alerts
Alert icons appear in cautionary and warning paragraphs in this guide to call attention to safety hazards.

Types of Safety Hazards
Most alert icons depict the relevant type of safety hazard.
Table 1. Safety symbols

Alert Icon

Explanation
General — Indicates a potential hazard requiring special attention. This icon
appears when the hazard or condition is of a general nature.

Electrical Hazard — Indicates a potential hazard requiring special attention when
you are working with electricity or electrical equipment.

Extreme heat and flammable materials
Indicates a potential hazard requiring special attention when you are working withextreme heat and flammable materials.

Instrument Guide | 17

Chapter 1 Safety and Regulatory Compliance

Table 1. Safety symbols, continued

Alert Icon

Explanation
Radiation hazard
Indicates a potential hazard requiring special attention when you are working with
UV radiation.

Levels of Potential Risk
Each alert icon appears in a paragraph type in this guide that indicates the level of potential risk posed by
the hazard or action described.

Cautions
A caution (example shown below) alerts you to take or avoid a specific action that could result in loss ofdata or damage to the instrument. A caution can also indicate that minor or moderate injury might occur if
the precaution against a potential hazard is not taken.
Caution: With the exception of cleaning the LED lamps, refer all servicing to qualified
Bio-Rad personnel or their agents.

Warnings
A warning (example shown below) precedes an action that, if not followed correctly, could cause serious
injury or death to the operator, serious or total loss of data, or serious damage to the instrument.
WARNING! Radiation Hazard — Keep the UV shield open for as little time as
possible.

18 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareInstrument Safety Warnings

Instrument Safety Warnings
Before operating the imaging system, carefully read the explanation for each safety icon.

Safety Icon

Explanation
Caution: With the exception of cleaning the LED lamps, refer all servicing
to qualified Bio-Rad personnel or their agents. If you experience technicaldifficulties with the instrument, contact Bio-Rad to schedule service. The
instrument should not be modified or altered in any way. Alteration voids
the manufacturer’s warranty and might create a potential safety hazard forthe user.
WARNING! The UV LEDs in the ChemiDoc Go imager are 279 nm +/5 nm, which falls between UV-B (280–315 nm) and UV-C (100–280 nm)
according to the World Health Organization (WHO).
If any interlock is defeated, there is a possibility of UV-B or UV-C radiation hazard
due to the UV light exposure. Exercise caution when servicing the instrument.

WARNING! This instrument must be connected to an appropriate ACvoltage outlet that is properly grounded.

The following warning label is also affixed to all imaging systems:

In the IEC/EN 62471 standard developed by the International Electrotechnical Commission, Group 3 isclassified as High Risk, where even momentary exposure to ultraviolet light (Actinic UV (200–400 nm)) ishazardous. Specifically, when viewing around the UV shield at a distance of 20 cm, the measured Actinic
UV emission is approximately 2.5 W/m2, with a permissible exposure time of about 12 seconds; where
even momentary exposure to Blue-light (300–700 nm) emission, when viewing without PPE at a distanceof 20 cm, the measured Blue-light (300–700 nm) emission is approximately 1.5 W/m2, with a permissible
exposure time of about 60 seconds.

Instrument Guide | 19

Chapter 1 Safety and Regulatory Compliance

Important: Ensure anyone using the instrument wears approved personal protective equipment(PPE).

Instrument Safety Cautions
Before operating the ChemiDoc Go imaging system, carefully read the following cautions. These cautions
are also included in the appropriate chapter in this guide.

Excising Bands from Samples
Caution: Before you begin excising bands, ensure that you have on the required
protective gear and that the UV shield is installed.

Caution: When excising bands on the UV/Stain-free tray, keep the UV shield open foras little time as possible.

Caution: Sharp cutting tools can easily damage the surface of the tray. To avoid this,place a sheet of clear glass or plastic on the tray before you add the gel sample. Use achopping motion rather than a sawing motion.

20 | ChemiDoc Go Imaging System with Image Lab Touch Software

Environmental and Safe Use Requirements

Environmental and Safe Use Requirements
The imager requires a space 38 x 37 x 48 cm (W x H x D) and a clearance of at least 8 cm from the backfor instrument ventilation and for connecting or disconnecting the AC power cord. Place the imager on a
sturdy and level laboratory bench or table away from excessive heat and moisture.
WARNING! Transilluminators are powerful sources of UV radiation, which can cause
serious damage to unprotected eyes and skin. The accessory UV shield provides some
UV protection; however, this shield does not protect others standing in the area aroundthe imager. Specifically, when viewing around the UV shield at a distance of 20 cm, themeasured Actinic UV emission is approximately 2.5 W/m2, with a permissible exposure
time of about 12 seconds; where even momentary exposure to Blue-light(300–700 nm) emission, when viewing without PPE at a distance of 20 cm, themeasured Blue-light (300–700 nm) emission is approximately 1.5 W/m2, with apermissible exposure time of about 60 seconds. Before performing band excision, theuser and other lab personnel in proximity to the imager must put on protective gear
including UV protective safety glasses, a face shield, lab coat, and gloves to ensurethat no skin is exposed. A typical and reasonable expectation of use is three operations
a day per user for three minutes each. Bystanders without protective gear must stand at
least 1.5 meters (five feet) away from the imager and limit their exposure to no longer
than one hour per day.
Table 2. Environment requirements for the ChemiDoc Go imaging system

Parameter

Specification

Installation site

Indoor use only

Operating altitude

Up to 2,000 m above sea level

Operating voltage

100–240 VAC; 50/60 Hz; 250 W

Operating temperature

10–28°C
50–82.4°F

Operating humidity

20–80% relative humidity (noncondensing)

MAINS supply voltage fluctuations± 10%

Overvoltage category

II

Pollution degree2Instrument Guide | 21

Chapter 1 Safety and Regulatory Compliance

Safety and Regulatory Compliance
Safety Compliance
The ChemiDoc Go has been tested and found to be in compliance with all applicable requirements of thefollowing safety and electromagnetic standards:n

IEC 61010-1:2010 Safety requirements for electrical equipment for measurement, control, andlaboratory use, Part 1: General requirementsn

IEC 62471:2006 Photobiological safety of lamps and lamp systemsn

CAN/CSA-C22.2 NO. 61010-1-12:2018 Safety requirements for electrical equipment formeasurement, control, and laboratory use, Part 1: General Requirementsn

EN 61010-1:2010 Safety requirements for electrical equipment for measurement, control, andlaboratory use, Part 1: General requirementsn

UL 61010-1:2012 Safety requirements for electrical equipment for measurement, control andlaboratory use — Part 1: General Requirements

Electromagnetic Compatibility (EMC)
The ChemiDoc Go has been tested and found to be in compliance with all applicable requirements of thefollowing electromagnetic compatibility standards:n

IEC 61326-1:2020 Electrical equipment for measurement, control and laboratory use — EMC
requirements — Part 1: General requirements. Tested as a Class A devicen

EN 61326-1:2013 Electrical equipment for measurement, control and laboratory use — EMC
requirements — Part 1: General requirements. Tested as a Class A devicen

FCC Part 15, Subpart B, Sections 15.107 and 15.109. Tested as a Class A digital devicen

CAN ICES-003v6:2019 Interference-causing equipment standard, information technology equipment(including digital apparatus) — Limits and methods of measurement. Tested to Class A limits

22 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareSafety and Regulatory Compliance

EMC Warnings and NotesnWarning: Changes or modifications to this unit, not expressly approved by Bio-Rad, could void theuser’s authority to operate the equipment.

nNote: This equipment has been tested and found to comply with the limits for a Class A digital device,pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. Thisequipment generates, uses, and can radiate radio frequency energy and, if not installed and used inaccordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference, in which casethe user will be required to correct the interference at their own expense.

nNote regarding FCC compliance: Although this instrument has been tested and found to comply
with Part 15, Subpart B of the FCC Rules for a Class A digital device, please note that thiscompliance is voluntary, for the instrument qualifies as an “exempted device” under 47 CFR 15.103(c), in regard to the cited FCC regulations in effect at the time of manufacture.

Instrument Guide | 23

Chapter 1 Safety and Regulatory Compliance

NoticeBio-Rad’s imagers are intended for laboratory use only. They are intended for use by specialized
personnel who know the health risks associated with reagents used in electrophoresis. The UV lightsource is computer controlled, and proper interlocks are implemented to avoid users’ accidental exposure
to UV radiation. Bio-Rad Laboratories, Inc. is not responsible for any injury or damage caused by use of
these instruments for purposes other than those for which they are intended, or for instrument
modifications not performed by Bio-Rad Laboratories, Inc. or an authorized agent.

Power Safety
The imager uses a universal power supply that automatically selects the correct voltage for your country
or region.
For the operating voltage requirement for ChemiDoc Go, see Environmental and Safe Use Requirements
on page 21.

Fuses
The imager has two user-serviceable fuses, F1 and F2, which are located on the rear panel and are a partof the power entry module. See Replacing the Fuses on page 175 for more information.

24 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 2 Introduction
The ChemiDoc Go Imaging System (known in this guide as the ChemiDoc Go imager) facilitates highsensitivity and high quality image acquisition for selected gel and blot applications. The imager uses
Image Lab Touch to control image capture and optimization. You interact with the imager using an
integrated touch screen and a simple user interface. Using Image Lab Touch, you can acquire and view
images and fine tune their appearance. You can then export images to a computer and analyze them with
Image Lab desktop software, or export them to the Bio-Rad cloud application, BR.io.
Note: Image Lab Touch does not support image analysis. Use the Bio-Rad desktop software ImageLab Software, version 6.1 or greater on a separate computer to analyze images acquired with theimaging systems.
The Bio-Rad imagers are compact gel/blot imaging instruments. These instruments automate theparameter selection for acquisition of high-quality and high-sensitivity gel and multichannel western blot
images with the tap of an on-screen button.
Using the ChemiDoc Go Imaging System, you can acquire data from 1–3 channels in a multichannel
image, including fluorescence, chemiluminescence, and colorimetric applications.

Product Features
The Bio-Rad imagers support the acquisition of images from a wide range of laboratory gels and blots.
The ChemiDoc Go imager features trans-UV, trans-blue, and trans-white as well as epi-UV and epi-white
excitation sources for detection of a large range of fluorophores and dyes.
Additional features includen

Support for colorimetric western blotting applications, chemiluminescent imaging, and DNA/protein
gel visualizationnMultiplex fluorescent western blotting applications (StarBright 520 and StarBright 700 dyes)nSmart, tray-based imaging that identifies the correct applications and presents appropriate filter andillumination sources for eachnDynamic flat fielding specific to each applicationnDynamic range of >4 orders of magnitudenConnectivity to the BR.io cloud application to send images from the ChemiDoc Go imager

Instrument Guide | 25

Chapter 2 Introduction

Using the BR.io app, you can download images onto your computer for use in Image Lab Software.
For more information about BR.io, go to BR.io. From the BR.io Home page, you canoCreate a BR.io accountoExplore the BR.io app via videooAccess helpful BR.io guides

Linking your ChemiDoc Go to your BR.io account requires an Internet connection. See your IT or
ChemiDoc Go administrator to connect your ChemiDoc Go to the Internet.

26 | ChemiDoc Go Imaging System with Image Lab Touch Software

Product Features

Front and Side Panel Components

LEGEND
1

Drip pad2

Touch screen3

USB 3.0 A port

4

System on/off button

5

Transilluminator drawer handle

6

Transilluminator drawer

Instrument Guide | 27

Chapter 2 Introduction

Rear Panel Components

LEGEND
1

Fuses

2

On/off switch

3

AC power receptacle

4

Ethernet port

5

USB 2.0 A ports

28 | ChemiDoc Go Imaging System with Image Lab Touch Software

Product Features

USB Ports
The instrument has three types of USB ports:nn

Use the USB 2.0 A ports on the back of the instrument to connect any of the following accessories tothe imaging system:
o

Mouse

o

Other peripherals

Use the USB 3.0 A port on the side of the instrument to connect a USB drive to quickly copy large
data files

Ethernet PortUse this port to connect the instrument to a network drive.
Note: The ChemiDoc Go imaging system cannot be controlled by an external computer.

CMOS Camera
ChemiDoc Go uses a fixed camera and lens to acquire samples. Samples are acquired at a field of view of
21 cm (long axis). Image Lab Touch crops the full view sample to obtain the smaller size samples.
Image Lab Touch uses a patented algorithm to acquire chemiluminescent samples. See Technical
Specifications on page 31 for more information.

Image Lab Touch Software
The imager ships with Image Lab Touch installed.

Emission Filters
The imager has a motorized emission filter for fluorescent and white light applications, which
automatically moves out of its position in front of the camera lens when not required. The imager uses aninfrared cut removable (IR cut) emission filter to image chemiluminescent samples.

Instrument Guide | 29

Chapter 2 Introduction

Optional Accessories
See Appendix D, Ordering Information, for a list of optional accessories and replacement parts.

Sample Trays
Table 3. Sample trays

Tray

Description

UV/Stain-Free tray

The UV/Stain-Free tray ships with the imaging system.
The UV/Stain-Free tray is used for applications that utilize UV
excitation sources. The tray is also used for chemiluminescentapplications.

White tray

The optional white tray is used for applications that utilize white
excitation sources such as coomassie gels or silver stained
samples.

Blue tray

The optional blue tray is used for applications that utilize blue
excitation sources, such as StarBright 520 and StarBright 700
fluorophores and SYBR® Safe and GelGreen dyes.

See Chapter 6, Choosing a Sample Tray, for more information.

30 | ChemiDoc Go Imaging System with Image Lab Touch Software

Technical Specifications

Technical Specifications
Table 4. Technical specifications
Supported Applications
Fluorescence

Yes

Chemiluminescence

Yes

Colorimetry

Yes

Hardware Specifications
Touch screen functionalityn Multitouch capable
n Display resolution 1024 x 768 pixels
n 9.7" (24.64 cm) display

Onboard computer systemn 4 GB RAM
n 256 GB disk spacen 4 USB ports (1 USB 3.0 port, 3 USB 2.0 ports)

Sample thickness

Maximum supported thickness: 5 mm

Maximum image arean Length: 14 cm
n Width: 21 cm

Excitation sourcen Trans-UVC (standard)n Epi-white (standard)n Epi-UV (standard)n Trans-white (standard, requires an optional white tray)n Trans-blue (standard, requires an optional blue tray

Detector

20.43 IMX183 CMOS

Pixel size

2.4 µm x 2.4 µm

Emission filter

n Clear (Chemi)
n 590/110
n 695LP

Dynamic range>3.7 orders of magnitude

Pixel density (gray levels)

65,535

Instrument Guide | 31

Chapter 2 Introduction

Table 4. Technical specifications, continued
Instrument sizen Depth: 48.0 cm (~19")n Width: 38.0 cm (~15")n Height: 37.0 cm (14.5")

Instrument weight

21.3 kg (~47 lbs)

Automation Capabilities
Workflow automated selection

Application driven, tray-based imaging and autoselection of excitation
sourceImage flat fielding

Dynamic; precalibrated and optimized per application

Autoexposure

2 user-defined modes (rapid or optimal)

32 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 3 Starting Image Lab Touch
Press the On button on the imaging system. The imaging system turns on and Image Lab Touch starts
automatically.
The Welcome screen appears.

About the Welcome Screen
On the Image Lab Touch Software Welcome screen, you can customize the text for your organization. For
example, you can enter the name of your business or laboratory in the upper box and add a warning
message or other helpful information in the second box. You can enter one line of text in the upper box,
and you can fill the lower box with text. The box does not scroll.

To edit Welcome screen text
1.

Press and hold on the text you want to change. The on-screen keyboard appears.

2.

Enter your text.

3.

Tap the keyboard key on the lower right to save your changes and close the keyboard. For
information on the keyboard, see Using the On-Screen Keyboard on page 35.

Instrument Guide | 33

Chapter 3 Starting Image Lab Touch

What's in the Suitcase?
Tapping the Suitcase in the lower right corner of the Welcome screen displays the Service Logout
command, which is for Bio-Rad Service personnel only:

Service Logout — enables service personnel to access the system for maintenance and service
procedures.

Interacting with the Touch Screen
In Image Lab Touch, you access imaging settings by tapping the setting type on the touch screen.
n

Tap an imaging settings tile to open dialogs in which you can specify imaging settings.

n

Tap an icon to execute a command or to open a menu or a view.

n

Tap a box to display an on-screen keyboard or numeric keypad in which you can enter data.

Table 5. Touchscreen actions
ActionDefinition

Tap

Briefly touch the screen surface.

Double-tap

Tap twice quickly.

Pan

Touch and then move your finger left or right.

Swipe

Slide a finger up, down, or across a box or screen to scroll contents.

StretchPlace a thumb and one or two fingers together on the screen, and then move themapart to zoom in.

Pinch

Place a thumb and one or two fingers slightly apart on the screen, and then movethem together to zoom out.

Alternatively, you can use a USB mouse to click tiles and icons or to click in boxes, and you can enter thedata with a USB keyboard.
Important: Plug in the USB mouse before you turn on the system to enable the mouse pointer toappear.

34 | ChemiDoc Go Imaging System with Image Lab Touch Software

Interacting with the Touch Screen

Tip: When you use a USB mouse, read the instruction tap in this guide as click.

Entering Text on the Touch Screen
You can enter or edit text in text boxes using the on-screen keyboard or keypad. You can also enter text
using a USB keyboard.

To access the on-screen keyboard or keypadu

Tap in a text box.
The appropriate on-screen input device appears.

Using the On-Screen Keyboard
Text boxes appear on the screen in which you can enter or edit text.
To display the keyboardu

Tap in a box to display the on-screen keyboard.

Use the on-screen keyboard or attach a USB keyboard to the imager to enter data.
You can switch between an English language and Simplified Chinese keyboard. Tap the Globe key on thebottom left of the keyboard to change keyboards and enter English alphabetic characters, numbers andsymbols, or Simplified Chinese characters.
Tip: You can change the system language to display all on-screen text in either English language orSimplified Chinese language.
To enter datau

Do one of the following:n

Tap the keys to enter data.

Instrument Guide | 35

Chapter 3 Starting Image Lab Touchn

Tap the Freehand key to display a black screen on which you can use your finger to print eachletter.

The letter appears in the text box when you lift your finger.
o

To return to the keyboard, tap the Aa key.

o

To return to the main screen, tap the Hide keyboard key.

36 | ChemiDoc Go Imaging System with Image Lab Touch Software

Interacting with the Touch Screen

Using the On-Screen Keypad
Text boxes appear on the screen in which you can enter or edit numerals or text. You can enter numerals
using the on-screen numeric keypad.

To display the numeric keypadu

Tap in a box.
The keypad appears on the screen.

To use the keypadn

Tap the text box to display the range of valid values below the box.

n

If a number you tap does not appear on the screen, verify that the number is within the range of valid
values. Invalid numbers are not accepted. If you enter an invalid number and tap Done, the box turns
red.

nSome properties are set by entering data in multiple text boxes. After you enter data in a box, tap thenext box. All edits in each box are saved automatically.

n

Tap Done or tap anywhere else on the screen to close the keypad.

Instrument Guide | 37

Chapter 3 Starting Image Lab Touch

Choosing Imaging Settings
Image Lab Touch displays an imaging settings tile for each channel image you want to acquire. For
example, when you tap Single, Camera view displays a single imaging settings tile in the left pane:

To choose your setttings
1.

Tap Single or Multi to acquire a single-channel or multichannel image.

2.

Tap Image Size to display image size settings and then select the image size you want.

38 | ChemiDoc Go Imaging System with Image Lab Touch Software

Interacting with the Touch Screen

The dimensions of the image size you select appear when you tap Image Size. Similarly, theapplication and exposure settings you select appear under Application and Exposure. For example,
when you tap Application in the tile, application categories and application types compatible with theselected application category appear:

Instrument Guide | 39

Chapter 3 Starting Image Lab Touch

Locking the System
If your imaging system has an administrator and if you log in with a password, you can lock the system.
This prevents other users from interrupting an acquisition or changing settings.
While the system is locked, only you (the current user) or an administrator can log you off the locked
system. If an administrator chooses to log you off while an action is in progress, the action and the lock arecanceled.

To lock the system during an acquisition1.

Tap Camera to start an acquisition.

2.

In the progress bar that appears, tap the Lock icon in the lower right corner.
The system locks and the acquisition continues.

To lock the system at any timeu

On the main toolbar, tap User Settings and then tap Lock Display.

The touch screen appears dimmed. The name of the current user and buttons for unlocking or
logging off the system appear in the upper right area.

40 | ChemiDoc Go Imaging System with Image Lab Touch Software

Locking the System

Unlocking the System
The user who locked the system (that is, the current user) can unlock it. An administrator can also unlock
the system by logging off the user who created the lock. When the user is logged off, the lock is canceled,
and the system is again available.

To unlock the system (current user only)1.

On the locked screen, tap Unlock.

2.

Enter your password in the box that appears.
The lock is canceled and you can continue to work with the system.

To log off the current user (administrators only)1.

On the locked screen, tap Admin Logoff.
The system prompts you to identify yourself as an administrator:

2.

Enter your user name and password and tap OK.
If an action is in progress, a message notifies you that data might be lost if you log off the current
user.

3.

Tap Yes to log off the current user or No to cancel.

Instrument Guide | 41

Chapter 3 Starting Image Lab Touch

Getting Help
Online help is available in each main view and in selected dialogs.

To access helpn

Tap the question mark icon in the main toolbar to open the help topic for the current screen.

n

Scroll to display longer topics and to access links to related topics.

n

Tap Help Topics at the bottom of any help topic to view a list of all main help topics.

To close Helpu

Tap the X in the upper right corner of the topic.

42 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 4 Logging In to Image Lab Touch
The first person to start Image Lab Touch on the instrument must create an account. The person cancreate a personal user account or an administrator account. The software prompts you to indicate
whether to be an administrator when you create the first account.
After the first person creates the first account, subsequent users can log into a general user account orcreate a personal user account. After the first Administrator account is created, only the administrator canassign the administrator account to other users.
Important: Until an administrator is designated, all user accounts have administrative privileges.
This chapter explains how to create the first accounts in Image Image Lab Touch.

Creating the First User Account
The first time you run Image Lab Touch, you must enter a user name to log in. The first person to log into
Image Lab Touch is prompted to choose whether the account will be an administrator.
Note: This section explains how to create the first user account the first time you run Image Lab
Touch. For information about creating the first administrator account when you first run Image Lab
Touch, see Creating the First Administrator Account on page 52.

To create the first user account1.

Tap anywhere in the Welcome screen.
The login dialog appears.

2.

Tap inside the text box.

3.

Using the on-screen keyboard that appears, tap in a user name and then tap Create.
The system prompts you to choose whether you want to be an administrator and displays informationthat helps you decide.

4.

Tap No.
Image Lab Touch opens to the default view.

Instrument Guide | 43

Chapter 4 Logging In to Image Lab Touch

Creating a Password
After an administrator has been appointed, the administrator can require users to log in with a password
and set other options to ensure the security of the system. Administrators always log in with a password.
Passwords must be 6–32 characters long and contain at least one of each of the following:nUppercase letternLowercase lettern

NumbernSpecial characterTo create a password
1.

Tap User Settings in the main toolbar.

2.

Tap Create Password. The Create password dialog appears.

44 | ChemiDoc Go Imaging System with Image Lab Touch Software

Creating the First User Account3.

Tap New password. The on-screen keyboard appears.

4.

Type a password.

5.

In the Confirm password box, enter the password again.

6.

Tap OK.

Changing a Password
To change a password
1.

Tap User Settings in the main toolbar.

2.

Tap Change Password.
The Change password dialog appears.

3.

Tap Current password. The on-screen keyboard appears.

4.

Type your current password.

5.

Enter a new password in the New password box.

Instrument Guide | 45

Chapter 4 Logging In to Image Lab Touch

6.

Enter the new password again in the Confirm password box.

7.

Tap OK.

46 | ChemiDoc Go Imaging System with Image Lab Touch Software

Creating the First User AccountRemoving a Password
If no administrator has been assigned, or if the administrator does not require users to log in with apassword, you can remove your password.

To remove a password
1.

Tap User Settings in the main toolbar.

2.

Tap Change Password in the dropdown menu.

3.

In the Change Password dialog, enter your current password.

4.

Leave the New Password and Confirm Password fields blank and tap OK.

Instrument Guide | 47

Chapter 4 Logging In to Image Lab Touch

Linking to Your Bio-Rad BR.io AccountNote: You must have a password on the ChemiDoc Go in order to connect to your BR.io account.
See Creating a Password on page 44 for more information. You must also set up a BR.io account.
For information about registering a BR.io account, see the help information in the BR.io app.
You can connect multiple ChemiDoc Go imagers to your BR.io account. When connected, you can send
images to the BR.io app and then download the images to your connected computer and view them in
Image Lab Touch.
Important: Before you can connect the ChemiDoc Go imagers to your BR.io account, the ChemiDoc
Go Admin must establish a connection to the Internet either through Ethernet or a wireless network
communication. For more information, see Configuring the Network Connection on page 165.
Tip: For more information about Bio-Rad‘s BR.io app, go to BR.io. From the Home page, you cannCreate a BR.io accountnExplore the BR.io app via videon

Access helpful BR.io guides

Connecting the ChemiDoc Go Imager to Your BR.io AccountTo connect the ChemiDoc Go Imager to your BR.io account1.

Tap User Settings in the main toolbar.

2.

Tap BR.io Account.
The system displays a message indicating the account is not linked.

48 | ChemiDoc Go Imaging System with Image Lab Touch Software

Creating the First User Account3.

Tap Link to link the account.
The ChemiDoc Go displays a message while it retrieves an authentication code. When retrieved, theLink to BR.io dialog appears.

Note: The authentication code is valid for one hour after it is retrieved. If you have not linked yourinstrument within that time frame, you will need to start again.
4.

Follow the on-screen procedure:a.

Open a web browser and navigate tobr.io/linkb.

At the prompt, type the code that appears on the Link to BR.io screen and click Submit.

c.

Follow the prompts on the upcoming screens to complete the linking process to BR.io.

Instrument Guide | 49

Chapter 4 Logging In to Image Lab Touch

5.

When the ChemiDoc Go successfully links to the BR.io the BR.io icon appears enabled on the menubar:

In the BR.io app, the Instrument Manager displays the connected instrument.
Note: When you log into the ChemiDoc Go after establishing the BR.io account connection, youare automatically connected to your BR.io account. When you log in to your BR.io account, yourimager appears in the instrument list.

Unlinking the ChemiDoc Go Imager from Your BR.io AccountIf you need to remove a ChemiDoc Go imager from your BR.io account, you must unlink your ChemiDoc
Go’s user account on that instrument from the BR.io app.
Note: You can link multiple ChemiDoc Go imagers to your BR.io account. Unlinking removes only thecurrent imager from your account. All other imagers remain linked to your BR.io account.

To unlink the ChemiDoc Go from your BR.io account1.

Tap User Profile.

2.

Tap BR.io Account.

3.

Tap Unlink.
A status dialog appears to confirm your ChemiDoc Go user account is unlinked from BR.io.

50 | ChemiDoc Go Imaging System with Image Lab Touch Software

Creating the First User AccountTroubleshooting BR.io Connection
If your instrument cannot link to or unlink from BR.io, you’ll see the message that follows:

To troubleshoot connection problems
1.

Confirm that your ChemiDoc Go system has internet connection.

2.

Confirm that you have a BR.io account.

3.

Confirm that you can access your BR.io account by logging in to the BR.io app.

4.

Restart your instrument and try connecting to BR.io again.

If the problem persists, contact Bio-Rad Technical Support.

Instrument Guide | 51

Chapter 4 Logging In to Image Lab Touch

Creating the First Administrator AccountThis section explains how to create the first administrator account during initial login. See Becoming an
Administrator after Initial Login on page 53.
The first user to log into Image Lab Touch can choose whether or not to be an administrator. If not, theuser can continue with a standard account.
Image Lab Touch provides a Make Me Admin in the user dropdown menu and the first user to select that
option becomes the administrator, setting up the administrative password and selecting administrator
options. After an administrator is appointed, all users must create passwords to log in.
Until an administrator is appointedn

Any user can become an administrator at any timen

All users can perform administrative tasksnUsers are not required to have passwords

After an administrator has been appointed, only the administrator can create other administrators, andother users’ privileges revert to those of a standard user.
Tip: Store your administrative password somewhere safe. For security purposes, administrative
password recovery requires the instrument to be serviced.

52 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareBecoming an Administrator after Initial LoginTo create the first administrator account during initial login
1.

Tap anywhere in the Welcome screen.

2.

Using the on-screen keyboard that appears, tap in a user name and then tap Create. The systemprompts you to choose whether you want to be an administrator and displays information that helps
you decide.

3.

To become an administrator, tap Yes and type a password in the text box that appears. The
Administrator Options dialog appears, in which you can choose options for managing Image Lab
Touch user accounts. For information, see Setting Administrator Options on page 54.

4.

Choose the options you want and tap Close. The imager opens to the default view.

Becoming an Administrator after Initial Login
To become an administrator after initial login
Note: This feature is available only if no other user has chosen to be administrator. If an administrator
is already appointed, only that user can give that role to other users.
1.

In any view, tap Make Me Admin in the User menu.

The Administrator Options dialog appears, in which you can choose options for managing Image Lab
Touch user accounts. For information, see Setting Administrator Options on page 54.
2.

Choose the options you want and tap Close.

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Chapter 4 Logging In to Image Lab Touch

Setting Administrator Options
Assigning the Admin role to a user enables the security and file access control features on the imaging
system. Table 6 and Table 7 on page 54 list the options you can set when you log in as an administrator.
Table 6. For All User Accounts
This Option

When Selected

Require Password

Requires all users to log in with a password. If the user has no password,
they must create one.

Require Approval for New

Requires administrator to approve all new user accounts.

Users
Restrict Deletion ofPrevents all users who are not administrators from deleting images.

ImagesRestrict Network Export

Restricts users to a top-level network location to which they might export

Locationfiles.

Require Secure File

When selected, the Require Password, Require Approval for New users, andExportRestrict Deletion of Images check boxes are also automatically selected, toensure secure file exports supporting regulatory standards.

Restrict Export to USB

When selected, only administrators can export files to a USB flash drive.

Enable Diagnostic Port

Enables Bio-Rad Technical Support to connect remotely to the imager for
diagnosing and troubleshooting issues.
For more information, see Enabling the Diagnostic Port on the ChemiDoc Go
Imaging System on page 57

Restrict Link to BR.io

Restrict users from creating a link to the BR.io app.
For more information, see Restricting Access to BR.io on page 58

Table 7. For Individual User Accounts
This Option

When Selected

Admin

Makes this user an administrator.

Disabled

Allows the administrator to disable a userʼs account. Only administrators canaccess disabled accounts.

54 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareBecoming an Administrator after Initial Login

This Option

When Selected
Note: If the user has linked the ChemiDoc Go imager to their BR.io
account, the imager is removed from that account when theadministrator disables the user. However, their account remains active
and any other linked imager remains linked.

Linked to BR.io

Indicates those users linked to BR.io.

Must Change Password

Requires this user to reset password.

Reset Password

Allows the administrator to enter a new password for a user.

Instrument Guide | 55

Chapter 4 Logging In to Image Lab Touch

To set administrator options
1.

Tap User Settings and then tap Admin Options.
The Administrator Options dialog appears.

2.

On the upper portion of the dialog, select the checkbox for each option you want to enforce.
Note the following:nSelecting Require Secure File Export Image Lab Touch automatically selects the Require
Password, Require Approval for New Users, and Restrict Deletion of Images checkboxes.

nSelecting Restrict Network Export Location opens the Select Network Folder dialog in which you:

n

3.

o

Enter the file path and tap Connect.

oEnter your network user name and password.

o

Tap OK.

Selecting Restrict Link to BR.io unlinks the ChemiDoc Go imager from all active BR.io accounts
and removes the imager from BR.io.

Clear the checkbox for each option you want to allow.

56 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareBecoming an Administrator after Initial Login

Enabling the Diagnostic Port on the ChemiDoc Go Imaging System
Important: Cybersecurity is the protection of assets in cyberspace from cyberattacks. Cybersecurity
is Bio-Rad’s ability to secure its people, information, systems, and reputation in cyberspace.
Cyberspace is the always-on, technologically interconnected world; it consists of people,
organizations, information, and technology.
Fast reaction is important with cybersecurity issues! If you suspect that there may be a cybersecurity
issue concerning your instrument or that cybersecurity has been breached at your site, contact your BioRad representative for technical support immediately.
To reduce cybersecurity threats, all TCP/IP ports on the ChemiDoc Go imager are closed by default. To
enable Bio-Rad Technical Support team to connect remotely for diagnosing and troubleshooting issues,
the ChemiDoc Go Administrator can temporarily open a diagnostic port on the instrument.
Note: Rebooting the instrument does not change the state of the port.
Important: Bio-Rad strongly recommends that you close the open diagnostic port after Bio-Rad
Technical Support completes troubleshooting the instrument to reduce any threat of cybersecurity
attacks.

To enable access to the diagnostic port
1.

Log in as an Administrator.

2.

Tap User Settings to display the Administrator Options screen.

3.

Select the Enable Diagnostic Port check box and then tap Close.

To disable the diagnostic port
1.

Log in as an Administrator.

2.

Tap User Settings to display the Administrator Options screen.

3.

Clear the Enable Diagnostic Port check box and then tap Close.

Instrument Guide | 57

Chapter 4 Logging In to Image Lab Touch

Restricting Access to BR.io
Users who have a BR.io account can connect multiple ChemiDoc Go imagers to their BR.io account.
When connected, users can send images to the BR.io app and then download the images to a connectedcomputer and view them in Image Lab Software.
The ability to link ChemiDoc Go imagers to BR.io is enabled by default. Administrators can restrict users
from linking to their BR.io account. When restricted, the BR.io icon no longer appears on the menu bar
and theBR.io Account menu item no longer appears in User Settings.
Important: When the option is selected, the ChemiDoc Go imager will be unlinked from all active
BR.io accounts and removed from BR.io. This cannot be undone. Ensure that all active BR.io users
understand this and are prepared to lose access to BR.io from the imager. Images currently saved on
BR.io will remain and users can access those images through Image Lab Software.

Restrict Linking to BR.io
To restrict linking to BR.io
1.

Log in to the ChemiDoc Go imager as an administrator.

2.

In the main toolbar, tap User Settings and select Admin Options.

3.

Tap Restrict Link to BR.io.

You see a dialog informing you that continuing unlinks the ChemiDoc Go imager from all active BR.io
accounts and removes the imager from BR.io.

58 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareBecoming an Administrator after Initial Login4.

Tap OK.
The BR.io icon no longer appears on the menu toolbar, the BR.io Access menu item no longer
appears on User Settings, and the imager is unlinked from all active BR.io accounts.

Instrument Guide | 59

Chapter 4 Logging In to Image Lab Touch

60 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 5 Understanding the Views
Image Lab Touch guides you through the processes of acquiring and managing images in four views:nCamera ViewnPreviewn

Image ViewnGallery

Color Cues in these views signal different states in the system. For information, see Color Cues on page70

Main Toolbar Settings
The main toolbar appears at the top of every Image Lab Touch view.

Tapping a toolbar object opens a view or a menu.
Table 8. Main toolbar menu
ObjectExplanation
Camera view — displays a view of the sample in the sample tray. Also displays apreview image of the sample.
Gallery — displays thumbnails of all images you acquired. You can view, browse, delete,or export images. Double-tapping a thumbnail displays the image full size in Image View.
Retake — visible when you acquire a multichannel image, enables you to retake one ormore channel images immediately after acquisition.

[Date andDate and Time — displays the current date and time. Your Image Lab Touchtime]administrator can change this setting in the System Settings menu.

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Chapter 5 Understanding the Views

Table 8. Main toolbar menu, continued
ObjectExplanation
BR.io Link Status— displays the status of the ChemiDoc Go connection to Bio-Rad‘s
BR.io app:n Instrument is not linked to BR.io
n Instrument is linked to BR.io
System Settings — menu enabling the user to set the following system options and to
calibrate the white tray:n Set the date and timen Set the time zonen Set the system language (either English, default, or Chinese)n Set sound volume
Tip: When the instrument identifies the white tray for the first time, a Calibration
dialog automatically appears.
Note: If an administrator exists on the ChemiDoc Go instrument, only theadministrator can access the Date and Time, Time Zone, and Language options.
Help — displays procedures and concepts for the view on screen.
User Settings — menu that accesses commands to change your password, set a filenaming format for acquired images, view Admin settings, lock the display, or log out.
Name of current user appears to its right.

62 | ChemiDoc Go Imaging System with Image Lab Touch Software

Camera View

Camera View
When you log in to the imaging system, Camera view displays the imaging stage as the default view. An
orange button with an exclamation point on the bottom left corner indicates that you must take an action
before acquiring an image. Tap the button to find more information.

When you place a sample in the imaging system, Camera view displays the sample as it appears in thecurrent tray. From the left pane, you can choose imaging settings such as image size, application, andexposure, for each channel you want to acquire.
After you choose settings that are compatible with the inserted tray, the orange button changes to a green
button with a camera and you can acquire the image.
The acquired image appears full size in Image View and also as a thumbnail in the Gallery.
nMain toolbar — displays access to Camera view or the Gallery, status of BR.io connectivity, andaccess to system settings, help topics, and user settings. For more information, see Main Toolbar
Settings on page 61.

nImaging area — displays the sample in the imaging system.

Instrument Guide | 63

Chapter 5 Understanding the Views

nImage Settings tiles — tapping Application or Exposure in a tile displaysoSingle/Multi - select either single-channel and multi-channel imaging modes.

oImage Size — select small, medium or large predefined image areas as well as the option to
manually define an image area size.

oApplication/Channel — select an application and channel type for the stain used and tray
inserted in the imager.

noExposure — set image exposure time to be set automatically or manually.

oPreview — quickly displays an image that you can use to optimize acquisition on a specificsample region when using automatic exposure.

Camera button — tapping this button at the bottom left of the screen acquires an image of thesample.

For more information, see Acquiring an Image — General Steps on page 80.

64 | ChemiDoc Go Imaging System with Image Lab Touch SoftwarePreview

Preview
Preview quickly displays image of the sample in the imager so you can determine whether sufficient
sample is visible and identify the region for which you want to set an automatic exposure.

Instrument Guide | 65

Chapter 5 Understanding the Views

Image View
When you acquire an image, it is automatically saved to the Gallery and opened in Image View. The
image automatically opens in full-size view.

66 | ChemiDoc Go Imaging System with Image Lab Touch Software

Image View

The Image View Toolbar
The Image View toolbar appears at the bottom of Image View. Its options enable you to fine tune andmanage images.

Table 9. Image View Icons

Icon

Description
Visible when a single image is displayed. Tap to scroll through Previous and Next images
selected for display in the Gallery.
Copy Zoom — visible when multiple images are displayed. Selecting a zoomed image
and then tapping this icon copies the zoom setting of the selected image to the otherdisplayed images.
Transform — enables you to optimize image appearance by adjusting brightness andcontrast.
Image Info — lists acquisition details for the active image and the name of the user who
acquired it. You can rename an image or add notes about it.
Merge — combines up to three images into a multichannel image.

Delete — deletes the displayed image.

Send/Save — saves the displayed image to a USB drive or network drive.

Instrument Guide | 67

Chapter 5 Understanding the Views

The Gallery
The Gallery displays thumbnail images of all acquired images, ordered and grouped by date acquired.
The most recent acquisition date appears first. The default name is <user name> <date and time ofacquisition>. You can change this name in the Image Info box.

Tap a thumbnail image in the Gallery and then tap the orange View button tonOpen the selected image in full size in Image View

nDelete selected imagesn

Save selected images to an attached USB drive, BR.io, or a network drive

Tap the zoom icons to increase or decrease the size of the thumbnails.

68 | ChemiDoc Go Imaging System with Image Lab Touch Software

The Gallery

To compare images, you can select up to four images in the Gallery to display in Image View.

For more information, see Comparing Images on page 119.

Instrument Guide | 69

Chapter 5 Understanding the Views

Color Cues
Image Lab Touch color cues signal you to the various system states.
Note: In this section, the color cue definition is followed by an example screen, in which a red outline
highlights the use of the color cue.
Blue indicates a selected object. In this image, Camera view and single image are selected.

In the Gallery and in Image View, selected images are outlined in blue.

Green and Orange indicate active objects. In the following graphic, the green color cue indicates that you
can select Gallery. The orange color cue indicates that you can create a multichannel image.

The image capture button on the bottom left corner appears in one of three colors:
n

Green

n

OrangenRed

70 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareColor Cues

Green indicates that the camera is ready; you can capture an image of the sample.

Orange indicates that an action is required before you can continue. In the next image, the orange
exclamation point appears in place of Camera to indicate that an error message requires action.

u

Tap the orange exclamation point to display a message that explains what to do.

Red indicates a critical error.

u

Tap the red exclamation point to display the message. You must log out of Image Lab Touch andrestart the imager to correct the error.

Instrument Guide | 71

Chapter 5 Understanding the Views

72 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 6 Choosing a Sample Tray
Before you acquire an image, you must select a sample tray that is designed for the application.
The imaging system supports the following trays:

Icon

Sample Tray Name
UV/Stain-Free tray

White Tray

Blue Tray

The imaging system automatically detects the type of sample tray inserted into the ChemiDoc Go imager.
If the tray in the transilluminator drawer does not support the application, an error message appears andthe imaging system does not acquire the image.
Note: The UV/Stain-Free tray is a universal tray for both the ChemiDoc and GelDoc Go imaging
systems. However, the blue and white trays are not interchangeable between the imagers.
ChemiDoc Go trays are identified by a blue or white triangle () on the label, depending on the tray

type. Ensure that you insert the blue or white tray that is appropriate for your imager.
Some fluorescent reagents have excitation peaks in both UV and blue wavelengths. In this case, either
excitation source can be used. However, the resulting images are not identical. Using the recommended
tray with these fluorescent reagents provides a better signal-to-noise ratio, which increases sensitivity.
Tip: When two tray types can be used, both are shown. An asterisk identifies the recommended tray.

Instrument Guide | 73

Chapter 6 Choosing a Sample Tray

To position a sample on the sample tray
1.

Place the sample face up and center it on the appropriate sample tray.

2.

Place the sample tray on the imaging stage.
Tip: Center the sample carefully so it fills the image area. If you enlarge the image by zoomingin, check to ensure that the sample is still centered.

3.

Close the transilluminator drawer.

74 | ChemiDoc Go Imaging System with Image Lab Touch Software

Applications Listed by Tray Type

Applications Listed by Tray TypeThe ChemiDoc Go ships with the UV/Stain-Free tray. Two optional trays are available for purchase.
Applications you can use with each tray are listed in the following tables.
n

UV/Stain-Free TraynBlue TraynWhite Tray

UV/Stain-Free Tray
Table 10 lists the applications for the UV/Stain-Free tray.
Table 10. Applications for UV/Stain-Free Tray

Applicationn Chemiluminescencen GelStar Stainn Colormetric blotsn Oriole Gel Stainn Coomassie Fluor Orange Stainn Ponceau blots

n Ethidium bromide (EtBr)n Stain-Free gels and blotsn Flamingo Stainn SYBR® Stains

n GelGreen Stainn SYPRO Ruby Stainn GelRed Stainn UView Stain

Blue Tray
Table 11. Applications for the Optional Blue Tray

Applicationn Colormetric Blotn StarBright Blue 520 Dye

n Diamond Stainn StarBright Blue 700 Dye

n Flamingo with XcitaBluen SYBR® Gold Stainn GelGreen Stainn SYBR® Green Stain

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Chapter 6 Choosing a Sample Tray

Table 11. Applications for the Optional Blue Tray, continued

Applicationn GelStar Stainn SYBR® Safe Stainn Stain-Free BlotWhite Tray
Note: The white tray must be calibrated before it is used for the first time.
Table 12. Applications for the Optional White Tray

Applicationn Coomassie Brilliant Blue Stainn Fast Blast DNA Stainn Silver Stain

76 | ChemiDoc Go Imaging System with Image Lab Touch Software

Applications Listed by Tray TypeCalibrating the White Tray
The optional white tray requires calibration before it can be used for the first time.
Note: If you subsequently obtain another white tray, Bio-Rad recommends that you calibrate the newtray.
When the imaging system detects that a white tray has been inserted for the first time, a message
indicates that the tray is not calibrated and lists steps to calibrate it.

To calibrate the white tray
1.

Complete the steps listed in the message box that appears.

2.

Tap Calibrate.
The system starts calibrating the white tray. A progress bar monitors the process.

A message notifies you when calibration is complete.
3.

Tap OK to close the message.

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Chapter 6 Choosing a Sample Tray

Calibrating a Replacement White Tray
When you replace the white tray, Bio-Rad recommends that you calibrate the new tray.

To calibrate a replacement white tray
1.

Tap User Settings, and then tap Calibrate White Tray.
A message displays the steps to calibrate the tray.

2.

Complete the steps listed in the message box.

3.

Tap Calibrate.
The system starts calibrating the white tray. A progress bar monitors the process.
A message notifies you when calibration is complete.

4.

Tap OK to close the message.

78 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 7 Acquiring Images
Instructions for acquiring an image apply to both single channel and multichannel images unless they arepreceded by (Single Channel) or (Multichannel).
The steps for acquiring single channel and multichannel images are similar. Acquiring a multichannel
image requires repeating some steps for additional channels, and a few procedures are specific to single
or multichannel images.
Before you can acquire an image, you must place a sample on the sample tray and then place the tray on
the imaging stage. You can specify acquisition settings before you place the sample on the sample tray orafter you place the tray in the transilluminator drawer. The imager retains your settings until you changethem.

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Chapter 7 Acquiring Images

Placing the Sample in the Imager
You must use the tray that supports the application you select. The imaging system detects the type ofsample tray on the imaging stage. If the tray in the transilluminator drawer does not support the selected
application, an error message appears and you will not be able to acquire the image.

To place the sample in the imager
1.

Place the sample face up on the sample tray.
Tip: If you plan to zoom in or out on the image, center the sample on the tray as accurately as
possible.

2.

Pull out the transilluminator drawer.

3.

Place the sample tray on the imaging stage.

4.

Close the transilluminator drawer.

Acquiring an Image — General Steps
These general steps outline the workflow for acquiring an image.
Note: You can configure 1–3 channels on the ChemiDoc Go for multichannel acquisition. The image
size you choose applies to all channels in the acquisition.

To acquire an image
1.

2.

In Camera view, do one of the following:n(Single Channel) Tap Single to configure a single-channel image.

n(Multichannel) Tap Multi to configure a multichannel image.

Specify the image size so that the sample fills as much of the screen as possible.

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Acquiring an Image — General Steps

3.

(Multichannel) Select a channel color or keep the default color. (You can change the channel color at
any time.)

4.

Choose the application.

5.

Preview the image (optional).

6.

Keep the default automatic exposure option or change the exposure options.
Note: When you set an automatic exposure time and specify a region of interest, the optimal
auto-exposure time applies to that region only.

7.

(Multichannel) Repeat steps 3–6 for the remaining channels.

8.

Tap Camera to acquire the image.

During acquisition, a progress bar appears on the screen. When acquisition completes, the image
appears in Image View. In Image View you cannAdjust the appearance of the imagenRename the imagen

Delete the image

For more information, see Chapter 11, Managing Images on page 129.
Tip: (Multichannel) If you cancel the process during multichannel acquisition, Image Lab Touch stops
acquiring images and stores the images that were acquired in the Gallery.

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Chapter 7 Acquiring Images

Setting Image Type and Size
Image type and size settings appear at the top of the left pane in Camera view. In multichannel
acquisitions, the image type and size you choose apply to all channels.

Choosing Image TypeTo choose an image typeu

Tap Single or Multi to acquire a single-channel or multichannel image.

Choosing Image Size
You can choose a preset image size or specify a custom size. The preset sizes correspond to these BioRad gels:nSmall — Mini-PROTEANn

Medium — CriterionnLarge — Pulsed Field Agarose

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Setting Image Type and Size

To choose a preset image sizeu

Tap Image Size to select a size in the dialog that appears.

The dimensions of the selected image size appear under Image Size.

To specify a custom image size for a gel or blot image
1.

In Camera view, tap Image Size.

2.

Tap the W (width) or L (length) box. When you enter one dimension, the software automaticallycalculates the other according to the image system’s 3:2 aspect ratio.

3.

Using the keypad, enter the width or length of the image (in cm).
Valid width values are 9.0–21.0 cm. Valid length values are 6.0–14.0 cm.

To change the image size manuallyuPlace a thumb and one or two fingers slightly apart on the screen and move them together (pinch tozoom) without lifting them from screen until the image is the size you want.

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Chapter 7 Acquiring Images

Choosing the Application
You can choose from a list of predefined applications for common sample types.
Image Lab Touch supports three application categories: nucleic acid gels, protein gels, and blots.
Multichannel images can be acquired only for blots.
Important: You must use the tray that supports the application you select. The imager detects thetype of sample tray on the imaging stage. If the tray in the transilluminator drawer does not support
the selected application, an error message appears when you try to acquire the image.
When you choose an application, the software selects the appropriate filter and illumination source.
Detailed settings appear in the imaging settings tile under the name of the selected application. The
default automatic exposure setting also appears. You can change the default exposure setting.

When you select a chemiluminescent application, the optimal binning level also appears under Exposure.
Note: ChemiDoc Go supports only chemiluminescent applications.

To choose an application
1.

In Camera view, tap Application in an imaging settings tile.
The Application box appears.

84 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChoosing the Application

2.

Under Choose Application Category, tap an application category.
The Choose Application list displays only applications compatible with the category you selected.
For example, when you tap Blots, only applications for blots appear.

3.

Tap an application.
(Multichannel) When you configure the first channel, the system eliminates applications that are
redundant or incompatible with the application you selected, and so on for the second and thirdchannels you configure.

4.

(Multichannel) Repeat steps 1–3 for each channel you want to add.

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Chapter 7 Acquiring Images

Single or Multichannel Images
Note: Only applications for blots are available for multichannel images.
You can configure 1–3 channels on the ChemiDoc Go imaging system for multichannel acquisition. The
image size you choose applies to all channels in the acquisition.
When you choose an application for a channel, a plus sign surrounded by a dotted outline tile appears
below the selected channel. Tapping inside the outline box changes it into an imaging settings tile for the
next channel.

(Multichannel) Selecting a Channel Color
Assigning channels different colors makes it easier to identify the channels in a composite image. You can
use any channel color with any application when you specify channel settings.
Note: There is no relationship between the emission wavelength of the sample and the channel
color.
Acquisition options for a channel display a default channel color. You can keep the default color or change
it. You can change a channel color at any time. If you change the channel color to one already assigned toanother channel, the channels swap colors.

To select a channel color
1.

Tap to view the color list.

86 | ChemiDoc Go Imaging System with Image Lab Touch SoftwarePreviewing an Image

2.

Tap a color to select it.

Previewing an Image
When you put a sample in the imager and close the drawer, Preview appears at the bottom of the imaging
settings tile.

When you tap Preview for an auto exposure, the system displays an image of the sample using theappropriate light source for the application.
View the image to identify a region of interest by resizing or moving the rectangle. If sufficient sample isvisible, you can then select either Auto Optimal or Auto Rapid exposure for the image you want to acquire.
Important: Keep the transilluminator drawer closed while viewing the preview image.

To preview an image
1.

Specify an application.

2.

Tap Preview to view an image of the sample.

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Chapter 7 Acquiring Images

3.

Inspect the preview image to verify that important sample features are visible in the sample.

4.

Use the grip on each corner to adjust the preview window and specify a region of interest (forexample a lane or band).

For information, see Specifying the Region of Interest for Auto-Exposure on page 92.
5.

Tap Camera to start the image acquisition.

To clear the preview image, do any of the following:nOpen the drawer.

n

Select an application that uses a different tray.

n

Switch between single and multichannel modes.

nLog out.

nDelete a channel and then add it again.

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Exposure Settings

Exposure Settings
Steps for specifying exposure settings are specific to the type of application you select, and how you
choose to expose it: automatically (Optimal or Rapid) or manually. Refer to the subsection about the typeof application you want to image.
n

Most gels and blotsnStain-Free gelsn

Chemiluminescent blots

Note: Chemiluminescence is the only chemiluminescent application available for the ChemiDoc Go
Imaging System. The system does not support other low-light applications.

Setting Exposure for Most Gels and Blots
To choose an automatic exposure setting for a gel or blot
1.

In Camera view, tap Exposure.

2.

Tap Auto, and then tap Optimal or Rapid Auto-exposure. For information, see Automatic Exposure
Settings on page 91.

3.

(For Stain-Free Gels Only) Tap Auto, and then tap Blot or Gel.
Note: Choose Blot if your Stain-Free gel will be used in later Western blotting. Choose Gel if
your Stain-Free gel will NOT be used in Western blotting.

4.

(Optional) Tap Preview, and then specify the region of interest in the preview image.

5.

Tap Camera to acquire the image.

To manually set an exposure time for a gel or blot
1.

In Camera view, tap Exposure.

2.

Tap Set Manual Exposure Time.

3.

Enter the time in the text box using the numeric keypad.

4.

Tap Camera to acquire the image.

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Chapter 7 Acquiring Images

Setting Exposure for Stain-Free Gels
Before you set the exposure time for a Stain-Free gel, you can select a gel activation setting.

To select a gel activation setting1.

Tap Exposure.

2.

Under Select Gel Activation, choose one of the following:nNone — to reimage a previously activated Stain-Free gel.

nBlot — to activate your Stain-Free gel for subsequent use in western blotting.

n

Gel — to activate your Stain-Free gel for maximum signal intensity.

To choose automatic exposure settings
1.

Tap Auto.

2.

Choose Optimal Auto-exposure or Rapid Auto-exposure. For information, see Automatic Exposure
Settings on page 91.

3.

Tap Camera to acquire the image.

To set a manual exposure time1.

Tap Manual.

2.

Tap the sec (seconds) box to display the keypad.

3.

Enter the exposure time in seconds.

4.

Tap Done.

5.

Tap Camera to acquire the image.

Setting Exposure Time for a Chemiluminescent Blot
Note: Chemiluminescence is the only chemiluminescent application available for the ChemiDoc Go
Imaging System. The system does not support other low-light applications.
You can choose automatic or manual exposure settings and adjust the binning level for theChemiluminescence application.
Image Lab Touch estimates the best exposure time for obtaining good-quality images with the optimal
dynamic range from the Chemiluminescence application.

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Exposure Settings

Automatic Exposure Settings
You can choose how Image Lab Touch calculates an automatic exposure. Two automatic exposure
settings, Optimal and Rapid, are available:nWhen you choose Optimal, the dynamic range of the image is maximized to show faint bands.
When you specify a region of interest, the dynamic range is optimized for the specified area only.

n

When you choose Rapid, the software calculates a reasonable exposure time more quickly than
Optimal by allowing a wider window of intensity to capture a reasonable image, but with a greater
chance of overexposed or underexposed images.

Tip: Using Rapid Auto-exposure can help you estimate manual exposure time. Choose Rapid
Auto-exposure and acquire the image. When the acquired image appears, tap Image Info in thelower toolbar to view the exposure time. Using this exposure time as a baseline, manually set the
exposure time to obtain the image quality you want.

Setting the Exposure Automatically for Chemiluminescent Applications
Note: Chemiluminescence is the only chemiluminescent application available for the ChemiDoc Go
Imaging System. The system does not support other low-light applications.
You can choose automatic or manual exposure settings and adjust the binning level for achemiluminescent fluorescence application (Far/Infra Red). Image Lab Touch software estimates the best
exposure time for obtaining good-quality images with the optimal dynamic range from chemiluminescentapplications.
By default, the software selects a binning level based on either the selected application or on a
combination of application and image size.

To choose an automatic exposure setting for a chemiluminescent application
1.

In Camera view, tap Exposure.

2.

Review the recommended binning setting on the scale and tap a different binning setting if needed.
Tip: Bio-Rad recommends starting with the optimal binning setting, examining the result, andthen adjusting settings when acquiring subsequent images.

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Chapter 7 Acquiring Images

LEGEND
Binning level
Selected binning setting
Optimal binning setting3.

Tap one of the following:nOptimal Auto-exposuren

Rapid Auto-exposure

4.

(Optional) Tap Preview to obtain a preview image and then specify the region of interest.

5.

Tap Camera to acquire the image.

Specifying the Region of Interest for Auto-Exposure
When you preview an image, you can specify a region of interest such as a band. Image Lab Touch sets
the automatic exposure so that the brightest pixels in the region of interest are exposed to near saturation,and uses that exposure for the entire image.
Note: You cannot specify a region of interest for a Stain-Free gel.
Consider specifying the region of interest when you want to do the following:n

To evaluate relative signal intensities, all features that you want to compare must be unsaturated. To
prevent saturation of features, specify a region that encompasses the features to be compared.

n

To push the limits of sensitivity, specify a region of faint or no apparent signal. The software optimizes
the exposure to reveal features in the specified region, but this might cause saturation of more
intense features on the blot.

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Exposure Settings

To specify the region of interest in a preview image
1.

Tap Preview in the imaging tile and wait for the light source to warm up.

A low-resolution image of the blot appears with a selection rectangle you can adjust to surround anarea of interest.
2.

To resize the rectangle, drag a corner.

3.

To move the rectangle, drag an edge.

4.

Tap Live to return to a view of the sample on the imaging stage.

5.

Tap Camera to acquire the image.

Setting the Exposure Manually
For a manual exposure time, the following options are available:n

Set Manual Exposure Time — to enter time manually in seconds.

nConfigure Signal Accumulation Mode — (chemiluminescent blots and single-channel only) tohave the imager capture a series of images with a range of exposure times.

To choose a manual exposure setting for a chemiluminescent blot
1.

In Camera view, tap Exposure.

2.

Tap Manual.

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Chapter 7 Acquiring Images

3.

Review the recommended binning setting on the Image Resolution/Time scale and tap a different
binning setting if needed.

LEGEND
Binning level
Selected binning setting
Optimal binning setting

4.

Tap Set Manual Exposure Time.

5.

Tap the sec (seconds) box to display the keypad.

6.

Enter the exposure time in seconds.
Tip: You can use the exposure time from a Rapid Auto-exposure image as a baseline for
estimating exposure time.

7.

Tap Camera to acquire the image.

94 | ChemiDoc Go Imaging System with Image Lab Touch Software(Single Channel) Acquiring Signal Accumulation Mode (SAM) Images(Single Channel) Acquiring Signal Accumulation Mode (SAM)
Images
SAM is useful for determining the best exposure time for a chemiluminescent sample.
SAM produces a series of cumulative images acquired using the range of exposure times you set. Afterusing SAM to determine the best exposure time, Bio-Rad recommends that you acquire a single image ofthe sample using the optimal exposure to reduce the amount of noise added by the accumulation process.
During SAM acquisitions, a preview of each image appears so you can monitor its quality.

To calculate the SAM exposure settinguEstimate the shortest and longest exposure times you think will achieve the optimal image.
For example, if you think the minimum time to acquire an image is 60 seconds and the longest time is
180 seconds, set these as the first and last values and then define the number of images to beacquired during the interval.
Tip: If no features appear in the resulting SAM images, acquire a second group of SAM images
using longer exposure times.

To acquire SAM images
1.

In Camera view, tap Exposure.

2.

Tap Manual and then tap Configure Signal Accumulation Mode.

3.

Enter the exposure times, in seconds, in the First Image and Last Image boxes.

LEGEND
Binning level
Selected binning setting
Optimal binning setting

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Chapter 7 Acquiring Images

4.

Enter the total number of images in the Images box. The recommended total is 3–5 images.

5.

Tap Camera to acquire the image.
The last image acquired appears full size in Image View.

To obtain the exposure time for the preferred SAM image
1.

In the Gallery, tap the SAM group icon.

Individual SAM image thumbnails appear.

2.

Tap the image with the exposure you prefer to select it.

3.

In the bottom toolbar, tap View Selected Images to display the selected image in Image View. The
image you selected appears full size in Image View.

4.

In the Image View bottom toolbar, tap Image Info to open the Image Information box.

5.

Under Acquisition Information, obtain the Exposure Time (sec) for the selected image.

96 | ChemiDoc Go Imaging System with Image Lab Touch Software(Single Channel) Acquiring Signal Accumulation Mode (SAM) Images

6.

Close the Image Information box.

To acquire an image using the best SAM exposure time1.

Tap Camera view.

2.

Tap Exposure and then tap Manual.

3.

Tap Set Manual Exposure Time.

4.

Tap the sec (seconds) box to display the keypad.

5.

Enter the Exposure Time value you obtained from the Image Information box.

6.

Tap Camera to acquire the image.

Adjusting SAM Acquisition Settings
You can preview SAM images as they are acquired and cancel the acquisition at any time. You can thenadjust the SAM settings and start SAM acquisition with the adjusted settings.
When you cancel a SAM acquisition in progress, the imager stops acquiring images and stores theimages already acquired in the Gallery. For example, if you set the total number of images to 10 andcancel while image 4 is being acquired, acquisition stops immediately, and images 1–3 are stored in theGallery.

To cancel SAM acquisition and adjust acquisition settings
1.

Carefully preview each image as it is acquired.

2.

If an image is not to your liking, tap Cancel.
The software stops the acquisition, stores acquired images in the Gallery, and displays the last image
acquired at full size.

3.

Go to Camera view and tap Exposure.

4.

Change the SAM settings.

5.

Tap Camera to start another SAM acquisition.

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Chapter 7 Acquiring Images

Acquiring the Image
After you specify the image settings, you can acquire the image. Make sure the transilluminator drawer ispushed in all the way and closed.
Note: If an exclamation point appears on the screen in place of the Camera, a problem is preventing
image acquisition. The color of the exclamation point indicates the severity of the problem. An orange
exclamation point indicates that an action must be taken before the image can be acquired. A red
exclamation point indicates that the camera is disconnected. Log out of Image Lab Touch and restart
the imaging system to correct the error.
If the problem persists, contact Bio-Rad Technical Support.

To view the error messageu

Tap the button in the bottom left corner of the screen.

A message appears explaining how to correct the error.

To acquire a gel or blot imageu

Tap Camera.
Acquisition starts. A progress bar monitors the acquisition.
When the image is acquired, it appears in Image View. You can adjust the appearance of the image.
You can also renameor delete the image in this view.
(Multichannel) If you cancel the process during multichannel acquisition, the software stops acquiring
images and stores the images that have been acquired in the Gallery.

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(Multichannel) Retaking an Image(Stain-Free Gel) Skipping Gel Activation
When you select a gel activation setting for a Stain-Free gel and tap Camera, gel activation starts before
acquisition. A preview of the image appears. You can monitor the progress of gel activation and stop it
when the gel is properly activated.

To stop gel activation and skip to acquisition
1.

Monitor gel activation carefully.

2.

When the gel is properly activated, tap Skip in the progress box.
The software skips the remaining time for gel activation and acquires the image.

(Stain-Free Gel) Acquiring an Image after Canceling Acquisition
Important: Do not open the drawer or move the sample.
When you activate a Stain-Free gel and then cancel the image acquisition in progress, you can use thesame gel, keep or change exposure settings, and acquire the image again.

To acquire an image after canceling acquisition1.

Tap Exposure.

2.

Select None.

3.

Set exposure options.

4.

Tap Camera to reacquire the image.

(Multichannel) Retaking an Image
You can retake one or more images in a multichannel image after acquisition. You can also delete one ormore channels to generate a different multichannel image. The new multichannel image results from the
combination of the remaining channel images.
Important: Do not leave the current view, open the drawer, or log out. Any of these actions removes
the Retake option from the main toolbar.
You can change the exposure settings and retake the channel image to achieve different results. You canalso change automatic or manual exposure options.
When you retake an image, Image Lab Touch does the following:n

Replaces the channel image you specify with a new image acquired using modified exposure
settings. The other channel images are retained.

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Chapter 7 Acquiring Images

nGenerates a new composite image by combining the channel image you retake with the channel
images you keep. (A composite image combines the data from the channel images into one image.)nStores the original multichannel image and the new multichannel image in the Gallery. (Amultichannel image consists of a composite image plus the channel images generated during one
acquisition.)

To retake a channel image
1.

Tap Retake in the main toolbar.

2.

Change the exposure settings in one or more channels.
The previous and current settings appear under Exposure in the imaging settings tile.

3.

(Optional) Select an area of interest.

4.

(Optional) To revert to the previous settings, tap Keep in the imaging settings tile.

5.

To retake the image, tap Camera.

100 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 8 Excising Bands from Samples
You can excise bands of interest from agarose or acrylamide gels for applications such as mass
spectrometry or DNA cloning.
The procedure for excising bands varies depending on the sample tray in use.
Caution: Before you begin excising bands, ensure that you have on the required
protective gear and that the UV shield is installed.

Caution: When excising bands on the UV/Stain-free tray, keep the UV shield open foras little time as possible.

Caution: Sharp cutting tools can easily damage the surface of the tray. To avoid this,place a sheet of clear glass or plastic on the tray before you add the gel sample. Use achopping motion rather than a sawing motion.

Excising Bands on a UV/Stain-Free TrayWARNING! Transilluminators are powerful sources of UV radiation, which can cause
serious damage to unprotected eyes and skin. The accessory UV shield provides some
UV protection; however, this shield does not protect others standing in the area aroundthe imager. Specifically, when viewing around the UV shield at a distance of 20 cm, themeasured Actinic UV emission is approximately 2.5 W/m2, with a permissible exposure
time of about 12 seconds; where even momentary exposure to Blue-light(300–700 nm) emission, when viewing without PPE at a distance of 20 cm, themeasured Blue-light (300–700 nm) emission is approximately 1.5 W/m2, with apermissible exposure time of about 60 seconds. Before performing band excision, theuser and other lab personnel in proximity to the imager must put on protective gear
including UV protective safety glasses, a face shield, lab coat, and gloves to ensurethat no skin is exposed. A typical and reasonable expectation of use is three operations
a day per user for three minutes each. Bystanders without protective gear must stand at
least 1.5 meters (five feet) away from the imager and limit their exposure to no longer
than one hour per day.

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Chapter 8 Excising Bands from Samples

Important: Ensure anyone using the instrument wears approved personal protective equipment(PPE).
Important: Before excising bands, you must install the UV shield.

To install the UV shield
1.

Pull out the transilluminator drawer.

2.

Place a UV tray on the transilluminator drawer surface.

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Excising Bands on a UV/Stain-Free Tray

3.

Place the shield over the tray:a.

Ensure the flap at the front of the UVshield is at a 90° angle.

b.

Insert the flap into the slot at the front of the drawer.
If the flap is inserted properly, the shield fits easily over the tray.

Important: Before you begin excising bands,ensure that you have on the required protective gearand that the UV shield is installed.
The UV lights turn off after 15 minutes of continuous use. To turn the UV lights back on, tap Turn
Transilluminator On.

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Chapter 8 Excising Bands from Samples

To excise bands on a UV/Stain-Free tray
Important: Before you begin excising bands, ensure that you have on the required protective gearand that the UV shield is installed..
1.

To avoid damaging the surface of the sample tray, place a sheet of clear glass or plastic on the tray
before you add the gel sample.

2.

Place a gel sample in the center of the tray.

3.

In Camera view, tap Turn Transilluminator On.
The screen dims and the transilluminator lights turn on, illuminating the gel. TheTurn Transilluminator
Off button remains active, all other screen options are not functional while the transilluminator is on.
Note: The UV lights turn on and the screen dims only when both the sample tray and the UVshield are in place. If the lights do not turn on, verify that the UV shield is installed correctly.

4.

Raise the UV shield no more than is necessary to work with the sample.
Caution: Keep the UV shield open for as little time as possible.

5.

Reach around the sides of the shield to excise the bands.

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Excising Bands on a UV/Stain-Free Tray

Caution: Sharp cutting tools can easily damage the surface of the tray. To avoid this,place a sheet of clear glass or plastic on the tray before you add the gel sample. Use achopping motion rather than a sawing motion.
6.

When you finish excising bands, tap Turn Transilluminator Off to turn off the UV lamps.
The screen no longer appears dim and the screen options become functional.

7.

Remove the UV shield, remove the sample tray, and slide in the transilluminator drawer.

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Chapter 8 Excising Bands from Samples

Excising Bands on a White Tray
Tip: Using the UV shield is not required when working with white trays.
Caution: Sharp cutting tools can easily damage the surface of the trays. To avoid
this, place a sheet of clear glass or plastic on the tray before you add the gel
sample. Use a chopping motion rather than a sawing motion.

To excise bands on a white tray
1.

Open the transilluminator drawer and place a tray on the transilluminator.

2.

Place a gel sample in the center of the tray.

3.

In Camera view, tap Turn Transilluminator On.
The screen dims and the transilluminator lights turn on, illuminating the gel. TheTurn Transilluminator
Off button remains active, all other screen options are not functional while the transilluminator is on.
Note: The lights turn on and the screen dims only when the sample tray is in place.

4.

Excise the bands.

5.

When you have finished excising the bands, tap Turn Transilluminator Off.
The screen no longer appears dim and the screen options become functional.

6.

Slide in and close the transilluminator drawer.

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Excising Bands on a Blue Tray

Excising Bands on a Blue Tray
Caution: Sharp cutting tools can easily damage the surface of the trays. To avoid
this, place a sheet of clear glass or plastic on the tray before you add the gel
sample. Use a chopping motion rather than a sawing motion.
Important: You must wear XcitaBlue goggles to see bands on a blue tray.
Note: Using the UV shield is not required when working with blue trays.

To excise bands on a blue tray
1.

Open the transilluminator drawer and place a tray on the transilluminator.

2.

Place a gel sample in the center of the tray.

3.

In Camera view, tap Turn Transilluminator On.
The screen dims and the transilluminator lights turn on to 75% intensity. TheTurn Transilluminator Off
and Intensity level buttons remain active, all other screen options are not functional while thetransilluminator is on.
Note: The lights turn on and the screen dims only when the sample tray is in place.

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Chapter 8 Excising Bands from Samples

4.

Tap the right or left Intensity arrows to change the brightness of the blue light coming from thetransilluminator.
The intensity level increases or decreases by 5%. You can change the intensity from 50 to 100%. The
system saves the selected intensity level for each user. Each time an individual user places a blue
tray on the transilluminator, the system automatically defaults to the intensity level that the user lastset. However, users can change the intensity level any time.

5.

Excise the bands.

6.

When you have finished excising the bands, tap Turn Transilluminator Off.
The screen no longer appears dim and the screen options become functional.

7.

Slide in and close the transilluminator drawer.

108 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 9 Viewing Images
The Gallery displays thumbnails of all images you acquire, in order by the date of acquisition, most recent
acquisition date shown first.
Note: Administrators can view all usersʼ images at once or view an individual userʼs images.

Accessing the Gallery
To access the Galleryu

Tap Gallery in the main toolbar.
A Gallery similar to the following appears.

You can change the size of the thumbnails in the Gallery by tapping the plus and minus zoom icons
on the right side of the Gallery screen.

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Chapter 9 Viewing Images

The image name appears below each thumbnail image. By default, each name consists of the username and the date and time of acquisition, for example: User1 2016-07-16 15hr7min33sec.scn.
You can change the file name in the Image Information box. You can also change the image nameformat.
See Changing the File Naming Format on page 130.

Searching for Images in the Gallery
You can search for images of interest in the Gallery. Search text is not case sensitive. You can enter any
part of the date or name. You can also search by application name or type.

To search for an image
1.

Tap the search box to display the keyboard.

2.

Enter search text. As you enter search text, images that match the search text appear in the Gallery.

3.

Tap Done.

To return to a thumbnail view of all your imagesuClear the search box.

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Selecting and Opening Images in the Gallery

Selecting and Opening Images in the Gallery
To select an imageu

Tap a thumbnail.
The selected image thumbnail appears outlined in blue and a check box appears in the upper rightcorner.

To open the selected imageuDouble-tap the selected image to open it full size in Image View.

To open 1–4 imagesuSelect 1–4 images and tap View x Selected Images in the bottom toolbar, where x is the number ofimages selected.
The selected images appear outlined in blue, and with checkboxes selected.

To select all images you acquired on a single dateu

Tap a date checkbox.
All images you acquired on the selected date appear outlined in blue, and with check boxes selected.

To select all of your imagesu

Tap Select All.
All images are selected and appear outlined in blue.

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Chapter 9 Viewing Images

Sorting Images in the Gallery by Date
By default, the Gallery shows all images you acquired, starting with the last image acquired on the dateyou specify. When you acquire another image, the Gallery displays that image and all other images in theGallery. You can filter the display by date to focus on images of immediate interest.

To filter images by date1.

Tap Show All in the image selection toolbar to open the dropdown list.

2.

In the Date dropdown list that appears, tap one of the following.
nDate Range — displays images acquired within a range of dates you specify. Tap the calendar
boxes to specify dates in any order.

n

Today — displays images you acquired today starting at 12:00:00 AM and ending at 11:59:59
PMn

Yesterday — displays images you acquired yesterday starting at 12:00:00 AM and ending at
11:59:59 PM.

nThis Week — displays images you acquired this week starting at 12:00:00 AM Sunday andending at 11:59:59 PM the following Saturday.

n

Last Week — displays images you acquired starting at 12:00:00 AM Sunday last week andending at 11:59:59 PM the following Saturday.

nThis Month — displays images you acquired this month starting at 12:00:00 AM of the firstcalendar day of the month and ending at 11:59:59 PM of the last calendar day of the month. For
example, the month of January starts at 12:00:00 AM January 1 and ends at 11:59:59 PM
January 31.

nLast Month — displays images you acquired starting at 12:00:00 AM of the first calendar day oflast month and ending at 11:59:59 PM of the last calendar day of last month.

112 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChecking Intensity Values

Checking Intensity Values
In Image View, you can display the average intensity value and the percentage of saturation for any spotin an image. A 3 x 3-pixel region of the image is averaged, except at the edge or corner, where a 3 x 2 or 2
x 2-pixel region is averaged.
When viewing a multichannel image, you can check intensity values in any of the channel views or in thecomposite view.
Note: Hiding a channel in a composite image also hides the intensity values for that channel.
You can use the saturation percentage to estimate how much to increase exposure time to maximize thedynamic range of the image. For example, if the spot is 50% saturated, doubling the exposure time resultsin about 100% saturation. Viewing intensity values is also useful when comparing images.

To view intensity values in an image
1.

In Image View, display an image.

2.

Tap any spot in the image.
Intensity values for the selected spot appear.

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Chapter 9 Viewing Images

Crosshairs mark the pixel area being evaluated. Intensity values fade out after a few moments.

To view intensity values in a composite image
1.

In Image View, display a multichannel image.

2.

Tap any spot in the composite image.
Color-coded intensity values appear for each channel in the composite image.

Crosshairs mark the pixel area being evaluated. Intensity values fade out after a few moments.

114 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareViewing Multichannel Images in Image View

Viewing Multichannel Images in Image View
After a multichannel image is acquired, the composite image and its individual channel images appear in
Image View. A blue border surrounds the selected image.

Images you open in the Gallery also appear in Image View. You can select and open up to four images at
a time in the Gallery, all of which appear together in Image View.

To view more than one multichannel image at a time1.

In the Gallery, select the multichannel images you want to view.

2.

In the lower toolbar, tap View x Selected Images, where x is the number of images you selected.
The multichannel images you selected appear in Image View.

To view an image full sizeu

Tap the expand arrows in the upper right corner of the image.

To view a single image in a multichannel imageu

Tap the Previous and Next arrows at the lower right corner of each image.

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Chapter 9 Viewing Images

116 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareViewing a Composite or Merged Image in Grayscale

Viewing a Composite or Merged Image in Grayscale
In Image View, you can view or export the composite or merged image in grayscale. In the Gallery, thethumbnail of the image also appears in grayscale. Channel colors of individual images are unaffected by
the grayscale setting.
By default, a composite or merged image appears in Image View in RGB, the combined color settings of
its channels.

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Chapter 9 Viewing Images

To view the image in grayscaleu

Tap the RGB toggle below the channel colors.
The toggle name changes to Gray and the image appears in grayscale.

118 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareComparing Images

Comparing Images
In the Gallery you can open and compare up to four images of a gel or blot at a time. The selected images
appear in Image View.

To open multiple images
1.

Select 1–4 images in the Gallery.

2.

In the bottom toolbar, tap View x Selected Images, where x is the number of images selected.
The images you select in the Gallery open in Image View. By default, the top left image is selected.

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Chapter 9 Viewing Images

120 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareCopying Image Settings

Copying Image Settings
It is easier to compare images that have the same transform and zoom settings. You can copy thesesettings from one image to the others you selected.

To copy zoom settings from one image to the others
1.

Select the image with the zoom settings you want to copy.

2.

In the bottom toolbar, tap Copy Zoom.

The pan and zoom settings of the selected image are copied to the other images.

To copy transform settings from one image to the others
1.

Select the image with the transform settings you want to copy.

2.

In the bottom toolbar, tap Transform.

The transform settings for the selected image appear.
3.

Tap Copy Transform in the transform settings box to copy the transform settings to the other images.
Copy Transform only applies to the images that are visible on the screen. Channels that are notvisible are not affected.

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Chapter 9 Viewing Images

Viewing Image Information
The Image Information box provides information such as the image name, the acquisition’s exposure
duration and date, and the application type.

To view information about the imageu

In Image View, tap Image Info in the bottom toolbar.
The Image Information box appears, with the image information for the selected channel displayed.
To see information for another channel in a multichannel image, tap that channel image and then tap
the Image Info button.

122 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareViewing SAM Images

Viewing SAM Images
When SAM images are acquired, the last image acquired appears full size in Image View. You can view
all the images in a SAM acquisition in the Gallery, where you can also rename, delete, or export the SAM
group.

To select a SAM acquisition1.

Tap Gallery.
The Gallery displays the stacked SAM images in a thumbnail. The file name appears below the
thumbnail. A stack icon in the bottom left corner identifies the thumbnail as a SAM acquisition.

2.

Tap the SAM stack thumbnail.

The bottom toolbar displays the number of SAM images in the acquisition:

To view all images in a SAM acquisition
1.

In the Gallery, tap the stack icon.

A thumbnail of each Image in the SAM acquisition appears.

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Chapter 9 Viewing Images

To view a SAM image in Image Viewu

Double-tap a SAM image thumbnail.
The image you selected appears full size in Image View.

To view up to four SAM images in Image Viewu

Tap up to four SAM image thumbnails and then tap View x Selected Images in the bottom toolbar,
where x equals the number of images you selected.
The selected images appear in Image View.

To restack SAM imagesu

Tap the SAM icon on any thumbnail in the SAM acquisition.

To clear a SAM image selectionu

Tap inside a SAM image thumbnail.

To clear all image selections and restack the SAM imagesuClear the checkbox in any thumbnail and tap its stack icon.

124 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 10 Merging Images
You can merge images to create multichannel images from up to three single images already acquired.
For example, you can merge a colorimetric image of prestained standards and a chemiluminescent imageof the same blot into a multichannel image. Original image data are not affected and can be used forquantitation as well as for determining the molecular weight or size of proteins. You can view merged
images in grayscale. File names for merged images use the format specified in User > File Naming
Preferences on the main toolbar.
Note: You cannot merge multichannel images. You cannot merge two or more SAM groups together,or merge a SAM group with a single image.

To merge images
1.

In the Gallery, select up to three images.

2.

On the lower toolbar, tap Merge.

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Chapter 10 Merging Images

The merged image appears in Image View.

To view merged image informationu

On the Gallery lower toolbar, tap Image Info. In the Image Information box, detailed information about
each image merged appears in Notes.

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Naming Merged Images
The system names the merged image by combining the application and exposure time for each image,and then appending the date the merged image was created.
If the name is already in use in the Gallery, the system generates another name by adding a hyphen andan incremental number (starting at 1) to the end of the combined name. The system continues to increase
the added number incrementally (-2, -3, -4 and so on) until the name is unique.

To view the names of the source imagesuWith the merged image open in Image View, tap Image Info in the bottom toolbar.
In the Image Info box, the Notes section displays the names of the images that were merged.

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Chapter 10 Merging Images

128 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 11 Managing Images
Adding Notes to an Image FileTo add information to an image file1.

In Image View, tap Image Info in the bottom toolbar.
The Image Information box appears.

2.

Tap in the Notes box.

3.

Enter your notes using the on-screen keyboard.

4.

Tap the keyboard key on the lower row of the keyboard to close the keyboard.
The notes you type are automatically saved.

For more information, see Viewing Image Information on page 122.

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Chapter 11 Managing Images

Changing the File Naming Format
By default, the file name consists of the elements user name, date, and time. In the File Naming
Preference dialog, you can change the file naming format at any time.
n

You can remove an element and replace it with another element.

n

You can include the Text element, which holds up to 32 alphanumeric characters.

n

You can reorder the elements.

To change the file naming format, you can select up to four of the seven elements in the File Name
Elements List and arrange them in the order you prefer.
Tip: The File Name Elements list includes a combination element, App/Exposure, which is usefulwhen you want to include more than four kinds of information in the format.

To change the file name format
1.

Tap User Settings, and then tap File Naming.

130 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChanging the File Naming Format

The File Naming Preference dialog appears. Default elements are User Name, Date, and Time.

2.

Do one or both of the following:n

To remove an element from the file name pattern, drag an element from the format onto the File
Name Elements list.

n

To add an element to the file name pattern, drag an element from the File Name Elements list
onto an empty position.

An example of the file name in progress appears as you select each element.
3.

Repeat Step 2 as applicable.

4.

Tap OK to save the format.
The software names all images you acquire using the new file naming pattern.

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Chapter 11 Managing Images

Renaming Images
When you acquire an image, the system assigns it a default name. You can change this name in Image
View or in the Gallery. In the Gallery, you can also save a series of images with the same name plus anincremental number. Doing so makes it easy to find related images.
An image name can consist of up to 190 alphanumeric characters. Spaces are permitted except at the
beginning and end.
Note: The following characters cannot be used: \ / : * ? “ < > |To rename an image in the Gallery
1.

Select an image.

2.

Tap Rename in the bottom toolbar.

3.

In the Rename dialog, enter a name for the image.

4.

Tap OK.

To rename multiple images in the Gallery
1.

Select more than one image.

2.

Tap Rename.

3.

In the Rename dialog, enter a name for the first image.

4.

Tap OK.
The system labels the remaining images with the same name as the first image plus a consecutive
number. For example: ChemiBlot_1, ChemiBlot_2, and so on.

To rename an image in Image View
1.

In Image View, tap Image Info.

2.

In the Image Information box, tap in the Name box to select the image name.

3.

Type a new name.

4.

Tap OK to save the image name.

132 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareRenaming Images

Renaming SAM Images
You can rename a SAM image or a SAM group. You can rename a combination of single SAM images,
SAM groups, and other images. Image and group names must be unique.
When you rename a SAM group each image in the group is also renamed. An underscore and asequential number are added to each image name to render it unique. The oldest SAM group or image isassigned the first sequential number. The remaining images are assigned sequential numbers, oldest
image first.

ExampleYou select a SAM group, SAM 8497, and another image in the Gallery.
You rename SAM 8497 to SAM 9001.
The software adds the suffix _1 to the renamed SAM group. The thumbnail for the SAM group now reads
SAM 9001_1.
Each image in group SAM 9001_1 is also renamed. The first image acquired is named SAM 9001_2. The
remaining images (including the image that is not in the SAM group) are renamed SAM 9001_3, SAM
9001_4, SAM 9001_5, and so on, in the order in which they were acquired. If the image outside the SAM
group was acquired before the acquisition of the SAM group, that image is renamed SAM 9001_1. In thiscase, the SAM group is renamed SAM 9001_2. The remaining images are renamed SAM 9001_3, and soon, in the order in which they were acquired.

To rename a SAM image
1.

Tap the SAM stack thumbnail.

2.

Select a SAM image.

3.

Tap Rename in the bottom toolbar.

4.

Enter a name in the Rename Image dialog and then tap OK.

To rename all images in a SAM group
1.

Restack the SAM images.

2.

Tap the SAM stack icon.

3.

Tap Rename.

4.

Enter a name in the Rename Image dialog and tap OK.

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Chapter 11 Managing Images

Printing Images
To print images acquired on the ChemiDoc Go imager, use Image Lab Software version 6.1 or greater.

To print an image
1.

Export the image to any of the following locations:n

The Bio-Rad cloud application BR.ion

An attached USB driven

A shared network drive

Tip: For information about exporting images, see Chapter 12, Exporting Images on page 137.
2.

In Image Lab Software, navigate to and open the target image and select Print on the main toolbar.

Deleting Images
In Image View, you can delete any open image. In the Gallery, you can delete multiple images at the sametime.

To delete images in the Gallery
1.

2.

Do one of the following to select images to delete:n

Tap one or more thumbnails.

n

Tap the checkbox for one or more days to select all thumbnails for those days.

n

Tap Select All to select all thumbnails in the Gallery.

Tap Delete and then tap OK.

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Deleting SAM Images

To delete an image in Image View
1.

2.

Do one of the following:n

If one image is open in Image View, tap Delete.

n

If more than one image appears in Image View, select the image you want to delete.

Tap Delete and then tap OK.

Deleting SAM Images
Tip: You can delete SAM images in the Gallery or in Image View.
In the Gallery, you can deleten

A SAM image or a SAM acquisitionn

A combination of single SAM images, SAM acquisitions, and other images

In Image View, you can deletenA full size imagen

An image you select from a view of up to four images

To delete images from a SAM acquisition in the Gallery
1.

Tap the stack icon next to a SAM stack thumbnail.

2.

Tap the thumbnail of one or more SAM images you want to delete.

3.

Tap Delete in the bottom toolbar.

4.

To confirm deleting the images permanently, tap OK.

To delete a SAM acquisition in the Gallery
1.

Restack the SAM images.

2.

Tap inside the SAM stack thumbnail.

3.

Tap Delete in the bottom toolbar.

4.

To confirm deleting the images permanently, tap OK.

To delete a combination of SAM images, other images, and SAM acquisitions in the Gallery
1.

Tap the stack icon next to each SAM acquisition that contains images you want to delete.

2.

Tap the SAM image thumbnails, non-SAM images, and SAM acquisitions you want to delete.

3.

Tap Delete.

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Chapter 11 Managing Images

4.

To confirm deleting the images permanently, tap OK.

To delete a SAM image in Image View
1.

2.

Do one of the following:n

If one SAM image appears in Image View, tap Delete.

n

If more than one image appears in Image View, select the image you want to delete and tap
Delete.

To confirm deleting the image permanently, tap OK.

136 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 12 Exporting Images
After acquiring images using Image Lab Touch, you can view and analyze your images in the Bio-Rad
desktop software Image Lab Software. To do so, first export the acquired images to a USB flash drive or ashared folder. Then copy the exported images to a computer running Image Lab software.
You can export a SAM group or individual SAM images. You can also export a combination of single
SAM images, SAM groups, and other images. SAM images and groups are stored in a folder inside the
top-level time-stamped folder:
You can export images from the imaging system to one of the following:n

Attached USB driven

Bio-Rad‘s cloud application BR.ion

Shared folder on a network drive or stand-alone computer

Image Lab Touch exports the images to a folder at the top level of the destination location. The folder isnamed ChemiDoc Images plus a time stamp, for example, ChemiDoc Images 2023-08-03_12.08.42. A
folder with a time stamp is created each time you export images.

For More Information
For more information about image analysis, see the Image Lab Software User Guide.

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Chapter 12 Exporting Images

Exporting Images
You can export images to a shared network drive, an attached USB drive, or to Bio-Rad‘s cloud
application BR.io.
Before you export to BR.io, you must set up a BR.io account and then link your ChemiDoc Go imager toyour BR.io account. For more information, see Linking to Your Bio-Rad BR.io Account on page 48
Before you can export images to a network, a shared folder must be present on the destination network,and you must know the UNC path to the shared folder. For more information, see Preparing to Export
Images to a Shared Folder on page 146.
You can export images from the Gallery or from Image View.

To export images
1.

Do one of the following:nSelect one or more images in the Gallery to export.

138 | ChemiDoc Go Imaging System with Image Lab Touch Software

Exporting Imagesn2.

Display up to four images in Image View and select an image to export.

Tap Send/Save on the bottom toolbar.
The available export locations appear on the top half of the screen.

3.

Select a target location.

4.

Under Export Options, choose the file formats in which you want to export the images.
Note: If you select Send to BR.io, you can only export to Image Lab. If you select any otheroption, Send to BR.io is disabled.

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Chapter 12 Exporting Images

A progress box monitors the export.
5.

When the export finishes, tap OK.

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Export Formats

Export Formats
You can export images to display them in a presentation, submit them for publication, analyze them inmore detail, or to a computer running Image Lab Software. Using Image Lab Software you can analyze
the images or set additional publication options. For more information, see the Image Lab Software User
Guide.
In Image View, you can export one image at a time. The software exports the current view of the image
when a publication format is selected. In this view, zooming and panning are considered.
In Gallery View, you can export multiple images at the same time. The software exports the full size of theimages in all selected formats.

Choosing Export Formats
To help you identify exported images the software appends application name in parentheses to the end of
the file name. This is especially helpful for multichannel images.

ExampleTable 13 on page 142 lists the file formats into which you can export images.

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Chapter 12 Exporting Images

Export File Formats
Table 13. Export file formats

Export Option

File Extension

Description

Image Lab

.scn or .mscn

Important: Only images in Image Lab format aresent to BR.io.
By default, Image Lab is selected. Image Lab Touch
exports a single-channel image in .scn format or amultichannel image in .mscn format.
To work with images in Image Lab software, use theseformats.

Publication.tif or .jpg

Important: Images in Publication format are notsent to BR.io. To send images to BR.io, selectImage Lab format.
Image Lab Touch software exports the current view ofan image in .tif or .jpg format at 300 DPI, which issuitable for publication. You can also insert an image in.tif or .jpg format into presentation software such as
Keynote or PowerPoint.
Tip: Additional publication options are available in
Image Lab software. For example, in Image Lab
you can export an image at up to 1200 DPI. To set
these publication options for the image, export theimage in the .scn or .mscn format, open the file in
Image Lab, set the options, and export the image
in .tif or .jpg.
Note: In a multichannel acquisition, the composite
image is exported as shown in the Image View
and/or Gallery View using the composite view
options (channel selection and gray/RGB color
selection).

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Exporting to BR.io

Export Option

File Extension

Description

Analysis.raw.tif

Important: Images in Analysis format are not sent
to BR.io. To send images to BR.io, select Image
Lab format.
Image Lab Touch software exports 16-bit .tif format(raw16.tif), which retains original pixel intensity datafrom the image acquisition. This raw image dataenhances quantitative analysis. You can also use thisformat to import the image into image editing softwarethat supports the 16-bit .tif format.
Note: You cannot export a composite image froma multichannel acquisition in .raw16.tif format.

Exporting to BR.io
Note: Only images in Image Lab format are sent to BR.io. If you select another format type, ImageLab Touch displays a message with this information and prompts you to send to BR.io or cancel. If
you select Send, your image is exported to BR.io in Image Lab format.

To export images in the Gallery to BR.io1.

Confirm that you have a BR.io account and that your ChemiDoc Go imager is linked to your account.

2.

From the Gallery, select the image or images you want to export.

3.

Tap Send/Save on the bottom toolbar.

4.

Tap Send to BR.io.

5.

Ensure that Image Lab is selected.

A progress box monitors the image export.
6.

When the export finishes, tap OK.

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Chapter 12 Exporting Images

To export an image in Image View to BR.io
Note: You can export only one image at a time in Image View.
1.

Double-tap an image to display it in Image View.

2.

Tap Send/Save on the bottom toolbar.

3.

Tap Send to BR.io.

4.

Ensure that Image Lab is selected.
A progress box monitors the image export.

5.

When the export finishes, tap OK.

Exporting to a USB Drive or External Hard Drive
The USB drive or external hard drive must meet the following requirements:n

Supports USB 2.0 or aboven

Is formatted with exFAT, FAT32, or NTFS formatn

Has no encryption software or other software add-ons on the drive

Note: USB drives formatted as exFAT or FAT32 export files much more quickly than USB drives
formatted as NTFS. If your USB export takes too long, reformat the USB drive as exFAT or FAT32.

To export images in the Gallery to a USB drive or external hard drive1.

Connect a USB drive or an external hard drive to the imaging system’s USB port.

2.

From the Gallery, select the images you want to export.

3.

Tap Send/Save on the bottom toolbar.

4.

Select export options.

5.

Tap Save to USB Drive.
A progress box monitors the image export.

6.

When the export finishes, tap OK.

To export an image in Image View to a USB drive or external hard drive
Note: You can export only one image at a time in Image View.
1.

Double-tap an image to display it in Image View.

2.

Connect a USB drive or an external hard drive to the imaging system USB port.

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Exporting to a USB Drive or External Hard Drive

3.

Tap Send/Save on the bottom toolbar.

4.

Select export options.

5.

Tap Save to USB Drive.
A progress box monitors the image export.

6.

When the export finishes, tap OK.

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Chapter 12 Exporting Images

Preparing to Export Images to a Shared Folder
Before you can export images to a stand-alone computer, a shared folder must be present on thecomputer, and you must provide the UNC path to that folder.
The UNC (universal naming convention) is a standard for identifying servers and computers on a shared
network. It is the full network path name of a folder, including the network server name. This path is thesame as a Windows folder path except that it uses double backslashes (\\) to precede the name of thecomputer.
Note: The procedures for determining the UNC path and creating a shared folder on it are based onrecent versions of Windows and Mac operating systems. If your computer is running on a different
operating system version, you might notice differences in the user interface.

Creating a Shared Folder on a Windows ComputerThis section explains how to create a shared folder on a Windows computer and how to determine its
UNC.
Note: In order to export images to a Windows computer, ensure that the imaging system isconnected to the stand-alone computer or to the network port by Ethernet cable.

To create a shared folder on a Windows computer1.

Log in as the Windows Administrator for the computer.

2.

Create a destination folder.

3.

Right-click the folder and select Properties in the menu that appears.

4.

Click the Sharing tab in the Properties dialog.

146 | ChemiDoc Go Imaging System with Image Lab Touch SoftwarePreparing to Export Images to a Shared Folder

5.

Under Network File and Folder Sharing, click Share.

6.

Choose users with whom you want to share the destination folder.

7.

Click Share and then click Done.

8.

(Optional) Set Read/Write permissions for each user.

9.

Click Close.

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Chapter 12 Exporting Images

To determine the UNC path to the shared folder
1.

On the Sharing tab, in the Network Files and Folder Sharing section the UNC path appears under
Network Path.

2.

Write down this path. You will enter this address when you export an image.

148 | ChemiDoc Go Imaging System with Image Lab Touch SoftwarePreparing to Export Images to a Shared Folder

Creating a Shared Folder on a Mac ComputerThis section explains how to create a shared folder on a Mac computer and how to determine its UNC.
Tip: The UNC for a Mac computer includes its Apple Filing Protocol (AFP) or Server Message Block(SMB).
Note: In order to export images to a Mac computer, ensure that the imaging system is connected tothe stand-alone computer or to the network port by Ethernet cable.

To create a shared folder on a Mac computer1.

Log in as the Administrator.

2.

Create a destination folder.

3.

Click the folder and choose Get Info in the menu that appears.
The <folder name> Info dialog appears.

4.

Select Shared Folder.

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Chapter 12 Exporting Images

5.

On the Apple menu, open System Preferences and select File Sharing in the Sharing dialog.

6.

In the Sharing & Permissions box, verify that the users with whom you want to share the folder havepermission to read and write to this folder.

7.

In the Service pane, select File Sharing.

8.

Under File Sharing: On, locate the SMB address and write it down.
You will enter the SMB address when you export an image.

9.

Click Options.

10. In the dialog that appears, select Share files and folders using SMB.
You can select both SMB and AFP. By default, OS X Mavericks and later systems automaticallyenable SMB and AFP for compatibility with Windows computers, Macs using Mavericks andYosemite, and Macs using older versions of OS X.

150 | ChemiDoc Go Imaging System with Image Lab Touch SoftwarePreparing to Export Images to a Shared Folder

11. Click Done.

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Chapter 12 Exporting Images

Determining the UNC of a Previously Shared Folder
In order to export images to a computer, you must provide the UNC path to the folder. This section
explains how to determine the UNC of a folder that has already been shared on Windows or Mac
computer.

Determining the UNC Path to a Network Folder on a Windows
ComputerIf the folder is located on a mapped network drive, you will need to determine the path to that drive as well.
Mapped network drives appear in Windows Explorer as simulated drives. This is indicated by a drive
letter, for example (H:). This section also explains how to determine the path to a mapped network drive
on a Windows computer.

To determine the UNC path to network folder on a Windows computer1.

Log in as the Windows Administrator for the computer.

2.

In the Navigation pane, click the Network icon and navigate to and select the destination folder.
The address bar displays the network path to the destination folder, beginning with the word Network.

3.

Click the address bar. Then UNC path appears in the address bar, for example:\\ImagesForEval

4.

Write down this path. You will enter this address when you export an image.

To determine the path to a mapped drive1.

Log in as the Windows Administrator for the computer.

2.

In the Navigation pane, click the network folder that contains the destination folder.
The address bar displays the mapped network path to the destination folder, beginning with the word
Computer, for example:
Computer > ILTuser (\\usherfs\users) (H:) > ImagesForEval

3.

To construct the UNC path, follow this rule:\\<shared_folder_name\mapped_foldername\destination folder
In this examplen\\usherfs\users — is the shared folder locationn

ILTuser — is the mapped folder name152 | ChemiDoc Go Imaging System with Image Lab Touch Software

Determining the UNC of a Previously Shared FoldernImagesForEval — is the destination folder

For example:\\usherfs\users\ILTuser\ImagesForEval

Determining the UNC Path to a Network Folder on a Mac Computer
Tip: The UNC for a Mac computer includes its Apple Filing Protocol (AFP) or Server Message Block(SMB).
Note: In order to export images to a Mac computer, ensure that the imaging system is connected tothe computer or to the network port by Ethernet cable.

To determine the UNC path to a network folder on a Mac computer1.

Log in as the Administrator.

2.

On the Apple menu, open System Preferences and select File Sharing in the Sharing dialog.

3.

Note the AFP or SMB path that appears below File Sharing: On. In this image, the SMB is 10.2.53.37.
This is the computer’s IP address, which is used to determine the computer name in the UNC path.

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Chapter 12 Exporting Images

4.

To construct the UNC path, follow this rule:\\SMB #\Users\<user_name\<folder_name\In this example
n10.2.53.57 — is the SMBnTester — might be the user namen

CDT Export — is the folder nameFor example:\\10.2.53.57\Users\Tester\CDT Export154 | ChemiDoc Go Imaging System with Image Lab Touch Software

Exporting to a Shared Folder for the First Time

Exporting to a Shared Folder for the First Time
Note: The following characters are not supported in paths to shared folders: * ? " < > | ;When you enter a path that includes an unsupported character, an error message appears. Folder namesthat include unsupported characters cannot be selected in browse results. You can rename the sharedfolder to exclude the unsupported character or choose another folder that does not include one of thesecharacters in its name.
Before exporting images to a shared folder on a network drive or a stand-alone computer for the first time,
obtain the following information:n

The UNC path to the shared folder on the network drive or stand-alone computernLog-in credentials to the shared folder

To export images to a shared folder
1.

Verify that the imager is connected via an Ethernet cable to a network outlet or to a stand-alone
computer.

2.

Do one of the following:nSelect the images you want to export in the Gallery.

n

Go to Image View by acquiring an image or by opening an image in the Gallery.

3.

In the bottom toolbar, tap Send/Save.

4.

Select Export Options.

5.

Tap Save to Network.
The Save to Network dialog appears.

6.

Type the pathname to the shared folder on the network drive or stand-alone computer.
Enter the network drive server name or IP address.
Server name pathname format: \\ServerName\Share1\Path\To\Folder

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Chapter 12 Exporting Images

Example: \\BioServer1\Share1\Images\Experiment1
IP address pathname format: \\IPAddress\Share1\Path\To\Folder
Example: \\10.1.52.162\Share1\Images\Experiment1
7.

Tap Save.

8.

If a log-in dialog appears, enter the log-in credentials to the shared folder. For a network drive, enter
the network domain name, your user name on the domain, and your password in this form:
domain_name\username\password
Example: Global.xyz.com\jdoe

9.

Tap OK.
A progress bar monitors the image export. When the export finishes, tap OK.

Exporting to a Shared Folder on a Network or Computer
Note: The following characters are not supported in paths to shared folders: * ? " < > | ;When you enter a path that includes an unsupported character, an error message appears. Folder namesthat include unsupported characters cannot be selected in browse results. You can rename the sharedfolder to exclude the unsupported character or choose another folder that does not include one of thesecharacters in its name.

Entering Log-in Credentials
When you export an image to a shared folder on a network drive or a stand-alone computer, you mustenter log-in credentials. After you connect to a shared folder, the connection remains active until you log
out.
Before you export images to a shared folder, obtain the following information:n

The UNC path to the shared folder on the network drive or stand-alone computer.

nLog-in credentials to the shared folder

If you do not have write permission to the shared folder, see your system administrator for write
permission.

156 | ChemiDoc Go Imaging System with Image Lab Touch Software

Exporting to a Shared Folder on a Network or ComputerTo export images to a shared folder
1.

Verify that the imager is connected via an Ethernet cable to a network outlet or to a stand-alone
computer.

2.

Do one of the following:n

In the Gallery, select one or more images to export.

n

In Image View, display an image to export.

3.

Tap Send/Save in the bottom toolbar.

4.

Select export options.

5.

Tap Save to Network and then tap Browse.

Do one of the following:nEnter the UNC path name to the shared folder on the network drive or stand-alone computer.
Tip: This is the path name you noted when you set up the shared folder. See either
Determining the UNC Path to a Network Folder on a Windows Computer on page 152 or
Determining the UNC Path to a Network Folder on a Mac Computer on page 153.

n
6.

Tap the path name you want under Save in the Save to Network dialog.

Tap Connect.
If you are not connected to the network drive or computer, the system prompts you for log-in
credentials.

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Chapter 12 Exporting Images

7.

Enter the log-in credentials to the shared folder and then tap OK.
For a network drive, type the network domain name, your user name on the domain, and your domain
password in this form:
domain_name\username
user password

8.

Tap Save to Folder. A progress box monitors the image export.

9.

When the export finishes, tap OK.

Exporting to a Recent Location
When you export images to a shared folder on a network drive or stand-alone computer, the software
saves the location you enter. The next time you export an image, the Save to Network dialog displays theten most recent locations you entered, most recent location first.

To export images to a recent location
1.

Verify that the imager is connected via an Ethernet cable to a network outlet or to a stand-alone
computer.

2.

In the Gallery or in Image View, select the images you want to export.

3.

Tap Send/Save in the bottom toolbar.

4.

Select export options.

5.

Tap Save to Network.
The Save to Network dialog appears.

158 | ChemiDoc Go Imaging System with Image Lab Touch Software

Exporting to a Recent Location

6.

Tap a shared folder in the recent locations list and then tap Save to Folder.
A log-in dialog appears if the connection to the network drive or stand-alone computer is not active.

7.

Enter the log-in credentials to the shared folder.
To enter the log-in credentials for a network drive, type the network domain name, your user name on
the domain, and your domain password. Type the domain and user name in this form:
domain_name\username
for example; Global.xyz.com\jsmith

8.

Tap OK.
A progress box monitors the image export.

9.

When the export finishes, tap OK.

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Chapter 12 Exporting Images

Removing Network Locations
To remove network locations from the listu

Tap a location and then tap Remove.

Disconnecting from a Shared Folder
The imaging system remains connected to a shared network folder until you log out or turn off the imaging
system or until the network disconnects you.

160 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareChapter 13 System Settings
The System Settings menu displays options that can be changed for your site. Users can view information
about the imager and change the system sound level when they log in.
Administrators can also set the following options for your site:nDate and timenTime zonenLanguage from English (the default) to Simplified ChinesenNetwork connection

This chapter explains how to set these options.

Setting the System Date, Time, and Time Zone
Before you begin to work with the imaging system, verify that the date and time settings are correct foryour locale.
Note: The date and time appear in the default names assigned to acquired images.
If your instrument is connected to a network you can enable the automatic date and time setting, which
synchronizes your system with the date and time information from an NTP server for your geographic
location.
You can also set a particular date and time by selecting a specific time zone.
If your instrument is not connected to a network, you can manually set the date and time.

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Chapter 13 System Settings

To automatically set the date and time
1.

Tap Settings in the main toolbar, and then tap Set Date and Time.

2.

Select the Set date and time automatically check box, and then tap OK.
Image Lab Touch sets the date and time based on NTP.

Important: When the checkbox is selected, you cannot manually change the date and time.

162 | ChemiDoc Go Imaging System with Image Lab Touch Software

Setting the System Date, Time, and Time Zone

To set the system time zone
1.

Tap Settings in the main toolbar, and then tap Set Time Zone.

2.

From the Select New Time Zone list, scroll to find the time zone for your locale.

3.

Tap the time zone to select it and then tap OK.

To manually set the current date and time
1.

Tap Settings in the main toolbar, tap Set Date and Time in the list that appears.

2.

Ensure the Set date and time automatically check box is cleared.

3.

In the Calendar box, tap the angle brackets to display the current month.

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Chapter 13 System Settings

4.

Tap the day of the month.

5.

Scroll through the Hour and the Minutes lists until the current time appears in the blue band.

6.

Scroll to position AM or PM in the blue band under AM/PM.

7.

Tap OK.
The selected date and time appear at the top of the all Image Lab Touch screens.

Setting the Sound Volume
You can change the system sound volume or turn off the sound.

To set the sound volume
1.

Tap Settings in the main toolbar.

2.

Tap Set Sound Volume in the list that appears.

3.

Tap a volume level.

4.

Tap X to close the list.

Setting the System Language
Note: Only those with the administrator role can change the system language.
You can change the system language from English language to Simplified Chinese. Image Lab Touch will
display all on-screen text in the selected language. It will also display the on-screen keyboard in theselected language.
Tip: You can choose to display the on-screen keyboard in either English language or Simplified
Chinese without changing the system language. See Using the On-Screen Keyboard on page 35 formore information.

To set the system language
1.

Tap Settings in the main toolbar.

2.

Tap Set Language in the list that appears.

3.

Tap the appropriate language.

4.

Tap X to close the list.

164 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareConfiguring the Network Connection

Configuring the Network Connection
Note: Only those with the administrator role can configure the network connection.
The ChemiDoc Go can connect to your internal network using either a static or dynamic IPv4 connection.
By default, the ChemiDoc Go’s IP connection is dynamic. That is, the IP address can change each timethe imager restarts. In these cases, the imager automatically establishes a new connection to the network
each time it starts. A static IP address never changes, thus the imager always reconnects to the same
network address each time it restarts.
This section explains how to view the dynamic or static IP networks settings, and how to set the network
connection.
Tip: If you choose to set a static connection, you might need the assistance of your systemadministrator for specific network settings.
Important: You must restart the imager to apply the changes.

To set the network connection
1.

Log into the ChemiDoc Go imager as an administrator.

2.

Tap Settings in the main toolbar.

3.

Tap About This Instrument in the list that appears. The imager’s information screen appears, forexample:Instrument Guide | 165

Chapter 13 System Settings

4.

Write down the IPv4 address located in the Network Connections section. You will need this number
to set a static connection.

5.

Tap X to close the system information screen.

6.

Tap Settings in the main toolbar.

7.

From the menu that appears, tap Network Configuration. The IPv4 Properties dialog appears.

8.

If your network supports a dynamic connection, tap the following options:nObtain an IP address automaticallynObtain DNS server address automaticallyNote: Automatically assigning an IP address will use a Link-Local address if a DHCP server is
not on the network.
9.

To set a static connection, tap Use the following IP address and Use the following DNS server
addresses options and then provide the IP address, subnet mask, default gateway, and DNS server
details for your site.

166 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareConfiguring the Network Connection

Tip: See your system administrator for the appropriate settings.
n

IP address — the IPv4 number that you noted in Step 4 on page 166.

n

Subnet mask — the numeric filter used to define the subnet to which the IP address belongsnDefault gateway — (required if you plan to access computers that are on a subnet other than theone on which the Imaging System belongs) this is the IP address of the node that allows
communication between the subnetsn

DNS server — the IP address of the node that translates a server name to its IP address

10. Tap OK to save the changes.
11. Tap X to close the dialog.

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Chapter 13 System Settings

168 | ChemiDoc Go Imaging System with Image Lab Touch Software

Appendix A Maintaining the Imaging System
Updating the Software and Firmware on ChemiDoc Go
Imagers
Note: If an Admin user exists on the ChemiDoc Go imager, only that user can perform the update. If
no Admin user exists, any user can update the system.
The update installer for ChemiDoc Go imagers can include software, firmware, and operating system
updates. Firmware updates for ChemiDoc Go imagers are not distributed in a separate package.
Bio-Rad offers two methods to access and install updates for ChemiDoc Go imagers:n

When connected to the Internet, the imager can detect and download updates from the ChemiDoc
Go product page on bio-rad.comn

You can manually locate and download the updates from the ChemiDoc Go product page on
bio-rad.com to a USB driveThis section explains how to perform both methods. Though optional, this section also explains how toverify the currently installed software and firmware versions.
Important: Depending on the type of update, the update process can take several minutes.

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Appendix A Maintaining the Imaging System

Verifying the Current Software and Firmware Versions
Tip: This section is optional. The update installer checks the current software version and displays
the information before continuing the process.

To verify the currently installed version
1.

Log on to the ChemiDoc Go imager and then tap System Settings on the main toolbar.

2.

Tap About This Imager to display the instrument’s information screen, for example:

3.

Note the currently installed software version.

4.

Tap X to close the system information screen.

Updating the Software and Firmware via the Internet
Note: Before starting the update process, ensure that no image acquisitions or exports are in
progress.

To update the software and firmware via the Internet
1.

(Optional) Confirm that the ChemiDoc Go imager has a network connection:a.

If you have not yet done so, log on to the ChemiDoc Go imager and then tap System Settings on
the main toolbar.

b.

Tap About this Imager.

c.

On the About screen, verify that the Network Connections field lists at least one network
connection.

170 | ChemiDoc Go Imaging System with Image Lab Touch Software

Updating the Software and Firmware on ChemiDoc Go Imagersd.
2.

Close the About screen.

On the main toolbar, open System Settings and then tap Update Installation.
The system displays a dialog prompting you to check for an update.

Tip: If you have never checked for updates, Last checked displays Never.
3.

Tap Check.
The system checks online for an update.

4.

If no update is detected, the system displays a dialog indicating the system is up to date. Tap OK toclear the dialog.

5.

If an update is detected, the system displays a dialog prompting you to download the update
package.

6.

Tap Download.
The system displays a progress bar while the download is in progress.

7.

(Optional) Tap Cancel to cancel the process.

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Appendix A Maintaining the Imaging System

Tip: You can restart the download process at any time by performing these steps again.
After the download process completes, the system displays a dialog indicating the current version of
the software, the update version, and prompts you to proceed with the update.
8.

Tap OK to proceed with the update.

9.

To complete the installation, follow the instructions that appear on the touch screen.
Important: Do not interrupt the installation process or turn off the imager while the update is inprogress.

Updating the Software and Firmware via USB Drive
To update the software and firmware via USB Drive
Important: Before the installation begins, the system prepares for the update. Do not remove theUSB drive until the update process completes.
1.

Obtain a USB drive and insert it into a USB port on a computer that is connected to the Internet.
Tip: Bio-Rad recommends you use an exFAT or FAT32-formatted drive.

2.

Log in to bio-rad.com and navigate to the ChemiDoc Go web page.

3.

Scroll down to Download and download the ImageLabTouchSoftware.swu file to the root directory of
the USB drive.

4.

Safely remove the USB drive from the computer.

5.

If you have not done so yet, log on to the ChemiDoc Go imager.

6.

Ensure that no image acquisitions or exports are in progress.

7.

Insert the USB drive into the USB port on the imager’s side panel.
Note: The system detects the ImageLabTouchSoftware.swu file on the USB drive and displays
a message indicating that an update is in preparation. Do not remove the USB drive until theprocedure completes.

8.

If the software version on the USB drive is higher than the software on the instrument, a dialog
appears prompting you to update the software.
Tap OK to proceed with the update.

9.

To complete the installation, follow the instructions that appear on the touch screen.
Important: Do not interrupt the installation process or turn off the imager while the update is inprogress.

10. When the installation completes, you can safely remove the USB drive.

172 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareEnsuring the Instrument is Level

Ensuring the Instrument is Level
The ChemiDoc Go instrument should be level to prevent samples from sliding on the sample tray andensure the best imaging. Place the instrument on the surface where you will be using it, and complete thesteps below.

To level the instrument1.

Open the drawer and place a sample tray on the surface.

2.

Retrieve the bubble level included with your instrument accessories.

3.

Place the bubble level on the tray, in the middle, as shown in the following graphic.

4.

Close the drawer.

5.

If necessary, power on the instrument.

6.

Tap the camera icon.
The image appears on the screen.
If the bubble is not in the middle of the inner circle seen on the level, you must raise or lower the
instrument’s feet to level it.

7.

Lift the instrument and rotate the feet as applicable to move the bubble to the center.
n

To raise the instrument, rotate the feet outward.

n

To lower the instrument, rotate the feet inward.

n

To move the bubble, rotate each foot inward or outward until the bubble appears in the middle ofthe inner circle on the screen.

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Appendix A Maintaining the Imaging System

Each time you adjust the feet you need to acquire a new image to view the new position of the bubble.
8.

Open the drawer slightly and then close it again to acquire a new image.
You do not need to tap the camera icon again.

9.

When the bubble is centered in the middle of the level, you can remove the level from the tray andclose the drawer.

Important: If you move the instrument to a new location, you must repeat this process to level itagain.

Cleaning the Instrument
During normal operation, residue can build up on internal and external areas of your ChemiDoc Go
instrument. On sample trays, residual contamination can affect image data and dust particles can glow
under UV illumination.
Use the instructions below to clean the following areas:n

Sample traysnTransilluminator glassn

Touch screennBlack rubber top matn

Plastic exterior

To clean the instrument surfaces and accessoriesu

Wipe down each surface using a standard laboratory detergent or mild solvent (such as 70% EtOH,
MeOH, or IPA).

Important: Note the following:n

For glass areas, use a nonabrasive lint-free towelette.

n

Do not clean sample trays in a dishwasher.

174 | ChemiDoc Go Imaging System with Image Lab Touch Software

Maintaining the LED Lampsn

For Coomassie stain, clean until no remaining stain is visible on the towelette.

n

You can safely submerge the rubber mat in water

Maintaining the LED Lamps
Note: The ChemiDoc Go imager uses custom LED lamps as the illumination source, which aredesigned to last the life of the instrument. The LED lamps are not serviceable or replaceable by thecustomer.

Replacing the Fuses
Replace the fuse with the following acceptable replacement fuse:

Name

NumberVoltage

Description

Schurter

0034.3123

250VAC

250 % 4A 5 x 20 mm

To replace the ChemiDoc Go fuses
1.

Unplug the power cord from the back of the instrument.

2.

Using a small flathead screwdriver, insert the tip to loosen the black fuse box cover.

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Appendix A Maintaining the Imaging System

3.

Pull out the red fuse box.

176 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareReplacing the Fuses

4.

Pull out the fuse box and gently pop out the fuse.

5.

Press the fuse box into place.

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Appendix A Maintaining the Imaging System

178 | ChemiDoc Go Imaging System with Image Lab Touch Software

Appendix B Important Imaging Concepts
This appendix provides additional information on important concepts for working with the ChemiDoc Go
imager.

Exposure Settings for Chemiluminescent Applications
You can use the Image Resolution/Time scale with adjustable binning settings to optimize the image youwant to acquire. Choose a lower binning setting (higher resolution) for higher-quality images. If bands are
faint, choose a higher binning setting for a shorter exposure time and the degree of sensitivity you require.
Image Lab Touch automatically determines the optimal setting required to achieve images suitable forquantitation or publication, as indicated by the Image Resolution/Time Scale. Images acquired at the
optimal setting have a resolution of 175 microns or more per pixel. Selecting lower bin settings increases
the resolution but might also increase the imaging time. Selecting higher bin settings reduces theresolution and exposure time but might result in a lower resolution than required.
Bio-Rad recommends that you start with the optimal binning setting shown in the Image Resolution/Time
Scale, examine the result, and adjust the setting for subsequent images.

Specifying the Region of Interest for Optimal Exposure
Preview displays a low-resolution image of the sample. When you select an automatic exposure setting,you can use the preview image to specify the region of interest by surrounding bands or other featureswith a selection rectangle.
Image Lab Touch acquires the entire image and sets the automatic exposure so that the brightest pixels in
the region of interest are exposed to near saturation and the rest of the image is exposed to show as muchdynamic range as possible. Specifying a region of interest is especially valuable when you are setting
automatic exposure for chemiluminescent or fluorescent samples.
Try specifying the region of interest for the following purposes:n

To evaluate relative signal intensities, all features that you want to compare must be unsaturated. To
prevent saturation of features, specify a region of the blot encompassing the area of interest.

n

To push the limits of sensitivity, specify a region of faint or no apparent signal. The software optimizes
the exposure to reveal features in the specified region, but might cause saturation of more intense

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Appendix B Important Imaging Concepts

features on the blot.

Adjusting How Images Are Displayed
Image Lab Touch optimizes images based on the range of intensity levels in the image and the known
behavior of the applications. Use this optimized image as a starting point. Use the histogram scale and thegrayscale curve settings in the Transform dialog to adjust the image brightness and contrast as
necessary.
Important: The transform settings change only the appearance of the image, not the underlying
data.
Note: Chemiluminescent images taken in SAM mode are displayed with a fixed transform so you cancompare them. The intensity is set to the bottom third of the full range, and the gamma is set to .75.
The fixed transform enables you to distinguish accurately the differences between the images in one
SAM acquisition so you can select the best one.

Adjusting Image Brightness and Contrast
Transform adjusts image brightness and contrast, optimizing the image display to highlight features ofinterest. The minimum to maximum range varies depending on the light and dark values present in theimage. Adjustments to brightness and contrast do not change the data. They change only the way thedata are displayed.
The frequency distribution histogram shows the total data range in the image and the amount of data at
each point in the range. The lightest part of the image is set to the minimum intensity, and the darkest is
set to the maximum.
Use the Low and High sliders to narrow the displayed grayscale range.
n

The High indicator determines the intensity value shown at the maximum value of the grayscale in thegel image.

n

The Low indicator determines the intensity value shown at the minimum value of the grayscale in thegel image.

n

The Gamma slider changes the grayscale curve. A value of 1 is linear. A value of <1 redistributes
more of the grayscale to the first half of the intensity values. A value of >1 redistributes more of thegrayscale to the second half of the intensity values.

A logarithmic histogram can reveal the presence of intensity values that are otherwise obscured. In
images with a large background area, the intensity value of most pixels is that of the background. Often,
there are too few pixels at the intensity values of the data peaks of interest to make these peaks readily
visible in a linear histogram.

180 | ChemiDoc Go Imaging System with Image Lab Touch SoftwareAdjusting How Images Are Displayed

A linear histogram can be useful when intensity values are more evenly distributed. Used in combination
with the logarithmic histogram, the linear histogram shows more clearly the relative quantity of pixels at
each intensity value.

To change the histogram scale
1.

Open an image in Image View and tap Transform.

2.

Tap the Log Histogram box to choose the logarithmic scale.

To change the light intensity range displayed in the imageu

Tap and drag the Low or High slider.
nDrag the Low slider to the right to obscure the background and low-intensity bands and to makehigh-intensity bands more visible.

nDrag the High slider to the left to make the background, nonspecific binding, and low-intensity
bands more visible. Intense bands might look overexposed.

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Appendix B Important Imaging Concepts

To adjust the grayscale curveu

Tap and drag the Gamma slider or touch anywhere in the slider bar.

Other Display OptionsnZoom in and out — displays greater detail about the intensity range.

nHighlight saturated pixels — pixels with saturated signal (higher than a measurable range) aredisplayed as red areas.

n

Invert image display — inverts dark and light areas. Clear the box to return to original display.

Restoring Default Settings
After changing the brightness and contrast settings for an image, you can restore the default settings.

To restore default settingsu

Tap Auto.

182 | ChemiDoc Go Imaging System with Image Lab Touch Software

The Image Resolution/Time Scale

The Image Resolution/Time Scale
The Image Resolution/Time scale includes six pixel resolution settings: 2x2, 3x3, 4x4, 6x6, 8x8, and16x16. The optimal binning setting required to achieve high-quality images is indicated by a check markon the Image Resolution/Time scale.

LEGEND
Binning level
Selected binningsetting
Optimal binning setting

Tap a different binning setting on the Image Resolution/Time scale to change the binning level.
When selecting acquisition options, you can view the selected binning setting under Exposure in theimaging settings tile, for example:

Instrument Guide | 183

Appendix B Important Imaging Concepts

How Binning Settings Affect Image Quality
Pixel binning refers to the process of combining data from adjacent CCD pixels to form a single larger
pixel. Doing so results in faster acquisition speeds and, in most images, improved light sensitivity.
However, pixel binning is achieved at the cost of reduced resolution. A binning of 2x2 means that 4
adjacent pixels are combined into one larger pixel. A binning of 3x3 means that 9 pixels are combined into
one, 4x4 means that 16 are combined into one, and so on.
Pixel binning combines adjacent pixels into a single larger pixel:

Using pixel binning, you can balance resolution and exposure time to acquire an image with the sensitivity
best suited for your purposes:nSensitivity — as binning increases, sensitivity to light increases in most images, which improves theability of the camera to detect faint bands. A binning of 4x4 uses 16 pixels compared to a binning of

184 | ChemiDoc Go Imaging System with Image Lab Touch Software

The Image Resolution/Time Scale

2x2, which uses four pixels. As a result, a binning of 4x4 is four times more sensitive than a binning of
2x2.
Note: At higher binning settings, sensitivity might depend on the signal intensity and size of thefeature being imaged relative to the background. If the bin contains more pixels than the feature(for example, a band), the intensity is averaged over the binned area. In some images, a higher
binning setting might render the feature not visible above the background.
nResolution — resolution decreases at all binning settings greater than 2x2. As pixel binning
increases, fewer pixels appear in the resulting image. As a result, resolution decreases more
noticeably at higher binning settings.

nExposure time — as pixel binning increases, it takes less time to acquire the image because larger
pixels collect more light. In addition, faster acquisition times accumulate less instrument backgroundwhich might improve visualization of faint bands.

Tip: Zooming in on the area to be imaged increases the resolution and might increase the optimalbinning. The loss in resolution with a higher binning setting can be offset by the smaller imaging area
and result in a shorter exposure time.
In general, a 3x3 binning setting produces an image that is relatively higher in resolution and requires
longer exposure times to achieve equivalent sensitivity to a higher binning setting.An 8x8 binning setting
usually produces an image that is relatively lower in resolution, higher in sensitivity (faint bands are
optimized), and takes less exposure time to produce.
Tip: For a first acquisition of a sample, you might use a higher binning setting (for example, 8x8) witha shorter exposure time. Evaluate the results and then use a 3x3 binning setting with a longer
exposure time on the next acquisition. With some acquisitions the 3x3 binning setting might produce
an image with optimal resolution and acceptable sensitivity.
The two gel lanes to the right of the image resolution/time scale show how an image looks given theselected resolution setting. As you tap on the different binning settings, the image displayed changes toshow the relationship between the resolution and sensitivity you select.
More intense bands with higher resolution appear at lower binning settings, for example:

Instrument Guide | 185

Appendix B Important Imaging Concepts

At higher binning settings, faint bands appear more prominent but resolution is reduced, for example:(Single Channel) Signal Accumulation Mode
Signal Accumulation Mode (SAM) is a semi-automated alternative for acquiring chemiluminescentimages. Rather than taking a series of single images with different exposure settings, SAM produces aseries of cumulative images. Each successive image includes all of the accumulated signal of theprevious images, plus additional exposure time.
To calculate the SAM exposure setting, estimate the shortest and the longest exposure times you think
will achieve the optimal image. Decide on the total number of images to be taken within this time range.
For example, assume you want to take four images. You specify a minimum exposure time of 60 seconds
and a maximum of 240 seconds. The first image is taken at 60 seconds, the last image is taken at 240
seconds, and the remaining two images are taken at even intervals in between, at 120 and 180 seconds.
SAM is useful for determining the optimal imaging time for a chemiluminescent sample. However, signals
near the intensity of background noise become less visible with each successive image. After you haveused SAM to determine the optimal exposure time, acquire a single image of the sample using the optimal
exposure to identify very faint signals in the image.

186 | ChemiDoc Go Imaging System with Image Lab Touch Software

Appendix C Free and Open-Source Notices for
ChemiDoc Go Imaging Systems
Bio-Rad’s ChemiDoc Go Imaging System products are developed using and incorporate or are bundledwith third-party materials (together, the “MATERIALS”) subject to free, open-source, or public-source
licenses (each an “OPEN LICENSE”). The terms of applicable OPEN LICENSES govern Bio-Rad’s
distribution and your use of the MATERIALS. Bio-Rad and the third-party authors, licensors, anddistributors of the MATERIALS disclaim all warranties and liability arising from all use and distribution of
the MATERIALS. To the extent the MATERIALS are provided under an agreement with Bio-Rad thatdiffers from the applicable OPEN LICENSES, those terms are offered by Bio-Rad alone.
The source code for MATERIALS included with ChemiDoc Go products may be downloaded frombio-rad.com/chemidocgo-opensource. The downloadable source code retains all copyright and otherlicensing notices originally included within the MATERIALS. Additionally, the reported license terms for
the MATERIALS are available via the Legal Notices link provided on the System tile on the ChemiDoc
Go’s About screen.
While Bio-Rad seeks to provide complete and accurate copyright and licensing information for all
MATERIALS, Bio-Rad does not represent or warrant that the copyright or licensing information iscomplete, correct, or error-free. MATERIALS recipients are encouraged to (a) investigate the identified
MATERIALS to confirm the accuracy of the licensing information provided and (b) notify Bio-Rad of any
inaccuracies or errors found in this document so that Bio-Rad may update this notice and the
downloadable source code accordingly.

Viewing the OSS License Text
To view the OPEN LICENSE textuOn the ChemiDoc Go Imaging System, navigate to Tools > About and tap Legal Notices on the
System tile.

Downloading the Open Sources Software
To download the open source softwareu

In a web browser, navigate tobio-rad.com/chemidocgo-opensource

Instrument Guide | 187

Appendix C Free and Open-Source Notices for ChemiDoc Go Imaging Systems

188 | ChemiDoc Go Imaging System with Image Lab Touch Software

Appendix D Ordering Information
Catalog #Description

Imaging Systems
12018023

ChemiDoc Go Imaging System with
Image Lab Touch

Analysis Software
12012931

Image Lab Software on a USB drive

17006130

Image Lab Software, Security
Edition, 1 license on a USB drive

17006171

Image Lab Software, Security
Edition, 5 licenses on a USB drive

17006172

Image Lab Software, Security
Edition, 10 licenses on a USB drive

ChemiDoc Go Imaging System Trays

12019594White Tray

12019595

Blue Tray

12012189

UV/Stain-Free Tray

Optional Accessories
12012164

UV Safety Shield for ChemiDoc Go
Imaging System

1708377

Holder for Sample Trays and UVShield1708185

XcitaBlue Viewing Goggles

12012190

ChemiDoc Go Gel Alignment Kit

Instrument Guide | 189

Appendix D Ordering Information

Catalog #Description

1703759

Bio-Rad Fluorescent Ruler

12012147

ChemiDoc Go Imaging System
IQ/OQ Kit

Replacement Parts
20002706

Fuse, 4A, 250 VAC

Electrophoresis Cells
1656001

Criterion Cell, includes verticalmidi-format electrophoresis cell,
buffer tank, lid with power cables,
3 sample loading guides

1658004

Mini-PROTEAN Tetra Cell for Mini
Precast Gels, 4-gel verticalelectrophoresis system, includes
electrode assembly, companion
running module, tank, lid withpower cables, mini cell buffer dam

Nucleic Acid Standards
1708351

EZ Load 20 Base Pair MolecularRuler

1708352

EZ Load 100 Base Pair Molecular
Ruler, 500 µl, 100 applications

1708353

EZ Load 100 Base Pair
PCR Molecular Ruler, 500 µl, 100
applications

Protein Standards
1610373

Precision Plus Protein All Blue
Standards, 500 µl, 50 applications

1610363

Precision Plus Protein UnstainedStandards, 1 ml, 100 applications

190 | ChemiDoc Go Imaging System with Image Lab Touch Software

Catalog #Description

1610374

Precision Plus Dual Color
Standards, 500 µl, 50 applications

1610376

Precision Plus Protein WesternC
Blotting Standards, 250 µl, 50
applications

Buffers1610732

10x Tris/Glycine/SDS, 1L

1610743

50x, TAE (Tris Acetic Acid/EDTA)
Buffer, 1L1610747

4x Laemmli Sample Buffer, 10 ml

Blotting Systems
1704150

Trans-Blot Turbo Starter System,
blotting instrument, includes base,
2 cassettes to hold 102 midi or up to
4 mini blotting sandwiches, blotroller1703940

Trans-Blot SD Semi-Dry
Electrophoretic Transfer Cell,semi-dry electrophoretic transfer
cell, includes agarose gel support
frame, extra thick blot paper in 4
sheet sizes

1703930

Mini Trans-Blot Electrophoretic
Transfer Cell, gel transfer cell, for
two 10 x 7.5 cm gels, includes 2
gel holder cassettes, foam pads,
electrodes, tank, blue cooling
unit, lid with cables

1704156

Trans-Blot Turbo Transfer Pack,mini, PVDF, pkg of 10

Instrument Guide | 191

Appendix D Ordering Information

Catalog #Description

1704157

Trans-Blot Turbo Transfer Pack,midi, PVDF, pkg of 10

1704158

Trans-Blot Turbo Transfer Pack,mini, nitrocellulose, pkg of 10

1704159

Trans-Blot Turbo, Transfer Pack,midi, nitrocellulose, pkg of 10

1704270

Trans-Blot Turbo, RTA Transfer Kit,
mini, nitrocellulose

1704271

Trans-Blot Turbo, RTA Transfer Kit,
midi, nitrocellulose

1704272

Trans-Blot Turbo, RTA Transfer Kit,
mini, PVDF

1704273

Trans-Blot Turbo, RTA Transfer Kit,
midi, PVDF

1704274

Trans-Blot Turbo, RTA Transfer Kit,
mini, LF PVDF

1704275

Trans-Blot Turbo, RTA Transfer Kit,
midi, LF PVDF

Detection Reagents
1705060

Clarity ECL Substrate, 200 ml, 25
blots

1705062

Clarity Max ECL Substrate, 100 ml,
15 blots

192 | ChemiDoc Go Imaging System with Image Lab Touch Software

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