Protein Quantitation

ChemiDoc™ Go Imaging System
Small Footprint, Big Results

Visit bio-rad.com/ChemiDocGo for more information.

ImpressiveImages.
Space-SavingSize.

Advanced chemiluminescence andStarBright™ Blue fluorescence detection
in a compact design. Go above andbeyond — acquire, save, quantitate,report, and publish with ease.
Smart Tray Technology* automatically recognizes your application.

Blue Sample Tray
(Optional)
GelGreen and SYBR®
stains; band excision.

White Sample Tray
(Optional)
Coomassie Blue, silver,and other stains.

UV/Stain-Free Sample
Tray (Included)
Chemiluminescentand fluorescent blots;
Stain-Free gels and blots;
ethidium bromide; SYPRO
Ruby and other stains;
band excision.

Scan the QR code and visualize thecompact ChemiDoc Go Imaging System
in your laboratory with our augmented
reality (AR) benchtop app.
(iPhone — worldwide; Android — U.S. only)

2 | BIO-RAD LABORATORIES, INC.

* U.S. patent 8,913,127.

Precision Chemiluminescenceand Blue-Fluorescence Imaging
ChemiDoc Go System

ChemiDoc MP System

8,388,608
4,194,304

Volume intensity

2,097,152
1,048,576
524,288
262,144
131,072
65,53632,76816,3848,192
1.00000.50000.25000.12500.06250.03130.01560.0078

Dilution factor

Chemiluminescent image analysis of a serial dilution seriesacquired with the ChemiDoc Go (left) and ChemiDoc MP(right) Imaging Systems.

Plot of signal intensity from a single band in a dilution series detected with theChemiDoc Go ( ) or ChemiDoc MP ( ) Imaging System.

Capture gel and western blot images from your benchtop
with the same sensitivity as with larger, shared instruments.
With advanced complementary metal oxide semiconductor (CMOS) digital imaging and high-intensity light sources,
the ChemiDoc Go Imaging System offers no compromises in sensitivity or dynamic range. Choose traditional
chemiluminescence or colorimetric detection or go further using multiplex analysis with cost-effective StarBright Blue
Fluorescent Secondary Antibodies. Normalize to total protein with Stain-Free gels in your western blot workflow andeliminate the need to detect housekeeping proteins.
The ChemiDoc Go Imaging System features high-intensity, all-LED illumination sources.
Transillumination

Epi-Illumination

UV LEDs: higher intensity at precise wavelengths

UV LEDs: specifically tuned for maximum Stain-Freeblot signal

Blue LEDs: higher-intensity, precise-wavelength
illumination for StarBright Blue 520 and 700
Antibodies, safe nucleic acid stains, and nucleic acidband excision

Green LEDs: higher-intensity, precise-wavelength
illumination yields greater signal intensity than“white” LEDs for most stains

Amber LEDs: higher-intensity, precise-wavelength
illumination yields greater signal intensity withCoomassie Blue and other visible stains

Example image of a multiplex
western blot using the Precision
Plus Protein Dual Color
Standards in the colorimetricchannel (blue) with StarBrightBlue 520 (green) and 700(red) Fluorescent Secondary
Antibodies imaged on theChemiDoc Go Imaging System.

BIO-RAD LABORATORIES, INC. | 3

Versatile Platform
Compact size with alarge imaging area.
The ChemiDoc Go Imaging System iscompatible with large-format gels and blots
and up to four mini-gels or blots at once.

Safe gel excision —no matter your stain.
Perform gel excision directly on the instrument.
The optional UV shield protects the user whenworking with traditional DNA stains.
Use the optional Blue Tray for easy direct
excision when working with safe stains.

You are in control — Smart Tray Technology
with light intensity adjustment.
The ChemiDoc Go System features a new toggle to adjust theblue light intensity when using the Blue Tray for band excision.

Supported Detection Methods
Protein Gels

Nucleic Acid Gels

Protein Blots

Stain-Free gels
Oriole Fluorescent Gel Stain
Silver stains
Coomassie Blue
Coomassie Fluor Orange
SYPRO Ruby
Flamingo Fluorescent Gel Stain

Ethidium bromide
SYBR® GreenSYBR® SafeSYBR® Gold
GelRedGelGreen
Fast Blast DNA Stain
UView Loading Dye and Stain

Stain-Free blots
Chemiluminescent blots
StarBright Blue 520
StarBright Blue 700
Colorimetric blots
SYPRO Ruby
Ponceau S
Negative stains

Visit bio-rad.com/GelExcision for a free Gel Excision Kit.

4 | BIO-RAD LABORATORIES, INC.

Designed to Perform
Three-Step Touch Workflow

Secure Cloud Storage

Using Image Lab Touch Software, choose the optimized
preset for your application, select Acquire, and save theimage in your choice of multiple file formats. Save to yourinstitution’s network, a USB drive, or your BR.io cloudaccount, and even print to a dedicated printer. Analyze
your data anywhere with Image Lab Software for Macor PC. Maintain electronic records for U.S. FDA 21
CFR Part 11 compliance with Image Lab Software,Security Edition for PC.

The first Bio-Rad imager connected to the BR.io
cloud platform, the ChemiDoc Go System simplifies
data storage, sharing, and analysis when images
are uploaded to your secure folders in the cloud.
Visit BR.io for more information on the powerfulcapabilities of the BR.io cloud platform.

StarBright Compatibility
Advanced CMOS Sensor Technology
After years of development, CMOS sensor technology
now meets the demanding requirements of life science
imaging, delivering higher efficiency and greater pixel
density than charge-coupled device (CCD) detection.
The new high-sensitivity CMOS sensors of the ChemiDocGo Imaging System also require less cooling and power,
enhancing reliability.

The ChemiDoc Go System now supports imaging
using StarBright Blue 520 and 700 Fluorescent
Secondary Antibodies.

All-LED Illumination
New in the ChemiDoc Go System are all-LED light sources
for trans- and epi-illumination. Multiple light sources provide
precise illumination for each application, and all-LED lights
eliminate the need for bulb replacements.

BIO-RAD LABORATORIES, INC. | 5

Complete Stain-FreeWestern Blotting Solution
Using Stain-Free technology in your western blot workflow
can help you achieve accurate results in less time.
With Stain-Free imaging, you can assess the quality of sample separation after electrophoresis and the efficiency
of protein transfer and then normalize your data to total protein — all without staining, destaining, or probing forhousekeeping proteins. Streamline your day with a ChemiDoc Go Imaging System on your bench.
Visit bio-rad.com/StainFree to learn more about Bio-Rad’s Stain-Free Western Blotting Workflow.

Sample Prep

Image Analysis

Electrophoresis

Image Acquisition

Transfer

Immunodetection

6 | BIO-RAD LABORATORIES, INC.

Consider FluorescentWestern Blotting
Fluorescently labeled secondary antibodies and advanced digital imaging
are a powerful combination for western blot detection and quantitation.
The ChemiDoc Go Imaging System allows fluorescence detection in two colors using StarBright Blue 520 and 700
Fluorescent Secondary Antibodies. Fluorescence detection with StarBright Blue Dye is compatible with total protein
normalization using Stain-Free technology, eliminating the need for a channel to detect housekeeping proteins.

Why StarBright Fluorescent
Secondary Antibodies
StarBright Blue fluorescent nanoparticles provide a
considerably brighter signal than conventional dyes andmatch the most sensitive chemiluminescent substrates.
These fluorescent nanoparticles are conjugated to highly
cross-absorbed goat anti-mouse (GAM) or goat anti-rabbit(GAR) IgG and are easily adapted to your current protocol.
Eliminate the need for detection substrates and takeadvantage of low background and a photostable signal
when using StarBright Blue Secondary Antibodies.

Why Multiplex Blotting
Multiplex blotting allows for the simultaneous detection
and quantitation of multiple proteins on a single blot. In
addition to saving time, the many advantages of multiplex
detection include:■

No stripping, reprobing, or cutting the membrane■

Conserving precious sample■

Generating more information from each blot■■

StarBright Blue 700
Blue-excited fluorescence
20 sec isualizing modified and unmodified targets withV
posttranslational modifications at one time uantitating proteins close in molecular weightQwith accuracy

StarBright Blue 520
Blue-excited fluorescence
20 secVisit bio-rad.com/FluorescentWestern for more
information on how to begin western blotting withfluorescent secondary antibodies.

BIO-RAD LABORATORIES, INC. | 7

Specifications

Ordering Information

Automation Capabilities

Catalog #Smart Tray Technology	The ChemiDoc Go Imaging System automaticallyrecognizes your application-specific tray and adjusts
imaging parameters and software options accordingly

Description

Maximum image area
(W x H)21 x 14 cm

Imaging System
12018025
ChemiDoc Go Imaging System, a blot and gel imaging systemcapable of imaging chemiluminescent, StarBright Blue 520 and700 fluorescent and colorimetric western blots, all common nucleic
acid and protein gel stains, and Stain-Free gels and blots. Includes
internal computer, 9.7" touch-screen display, camera, Image Lab
Touch Software, and UV/Stain-Free sample tray. White Tray,
Blue Tray, and additional accessories are sold separately
ChemiDoc Go Imaging Trays
12019873
White Tray for ChemiDoc Go Imaging System
12019748
Blue Tray for ChemiDoc Go Imaging System
12012189
UV/Stain-Free Tray for ChemiDoc Go andGelDoc™ Go Imaging Systems

Detector

20.48 MP back-side illuminated (BSI) CMOS

12012931

Image Lab Software on USB drive

Quantum efficiency

75% at 425 nm

17006130

Image Lab Software, Security Edition, 1 license

Bit depth

65,53517006171

Image Lab Software, Security Edition, 5 licenses

Dynamic range>4 orders of magnitude

17006172

Image Lab Software, Security Edition, 10 licenses

Auto-focus

Precalibrated focus for any zoom setting

Auto-exposure

Two auto-exposure algorithms (rapid or optimal)

Image flat fielding	Dynamic; precalibrated and optimized forevery application
Hardware Specifications
Touch-screen functionality	Multitouch capable
Display resolution 1,024 x 768 pixels
9.7" (24.64 cm) display

LED excitation sources	Trans-UVTrans-blue
Trans-amber
Epi-UV
Epi-green

Analysis Software

Optional Accessories
12012164

UV Shield for ChemiDoc Go and GelDoc Go Imaging Systems

1708377

Holder for Sample Trays and UV Shield

1708185

XcitaBlue Viewing Goggles

12012190

ChemiDoc Go and GelDoc Go Alignment Template Kit

Data output	Image Lab SCN, MSCN, SSCN, TIFF (16-bit or 8-bit),and JPEG

12019757

ChemiDoc Go Imaging System IQ/OQ Kit

1703760

Gel Cutter

Instrument weight12018650

ChemiDoc Go Imaging System WiFi Adapter, US Only12018509

ChemiDoc Go Imaging System WiFi Adapter, GlobalEmission filters

520/260 BP, 590/110 BP, 695 LP

21.3 kg (46.9 lbs)

Instrument size
(W x D x H)

36 x 48 x 36 cm

Operating voltage

100–240 VAC, 50–60 Hz

Operating temperature

10–28ºC

Operating humidity

20–80% relative humidity (noncondensing)

Visit bio-rad.com/ChemiDocGo for more information.
BIO-RAD, CHEMIDOC, GELDOC, and STARBRIGHT are trademarks of
Bio-Rad Laboratories, Inc., in certain jurisdictions. SYBR is a trademark of Thermo Fisher
Scientific Inc. All trademarks used herein are the property of their respective owner.
© 2024 Bio-Rad Laboratories, Inc.
Image Lab Touch software is based in part on the work of the CImg project (http://cimg.eu/).
See license for details at www.cecill.info/licences/Licence_CeCILL-C_V1-en.html.
Image Lab Touch is based in part on libraries from GCC runtime and the Gnu C library.

Bio-RadLaboratories, Inc.
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