,!))+&
 '%
 # *("$6<:»µºrº/]Vºº/½.*£*³I+£*³I(*]VºB=¹º,)¡dÀ,+H6<:[¯fevcRºPXeaX+s6<:[¯qÄ/U"!vcR+O,)/{M+

¼©+#v

cRº/MEM&,+(E/ªM
~vijº/·x+c.)6<:/»µ+>Hb¥

Q+º]VºBHb@¦/ºh_PXe®/t&Lp}¿),+[¯\Æ++Ê014H?´n¥/«J+z794342<:HbU
+

M+

*#,GÈZcR014H?/j+z

6<:»µ¼©+?Ãº6<:º¼©cR'vHb¥º8;54}¿+##I,+vcRºMÅnqÄ/U"

6<:[¯qÄ/U"wuºh_PXvcR+W¥v¶o+wu

+Hbº

ºy'+>kÁ6<:la°+Hb¥+|²´«Jz#,

?Ãº6<:º)HbK¦Hb@¦/SÂv±/§(*Hb@¦Mz/¾+/¬$?Ãº6<:º NBRC 2003 £*³`Ç`Ç)HbK¦Hb@¦/SÂAv/±-É¤vm­%),(1)

HbK¦@¦É¤vmD?Ãº6<:º&Y­%),
É¤v/N+«J+z/zU(*Hb@¦/|MºHb¥/¨?Ãº6<:ºHb@¦v¯MHb@¦T^¸Fg(2)¢C+(1) Yamagishi, H. et al. (2001) Biosci. Biotechnol. Biochem. 65 (10), 2361-2363.
(2) Yamagishi, H. et al. (2010) J. Gen. Appl. Microbiol. 56, 297-312.

!
"f±É®{2 `]ª®eÈ 4  1 `]®{¥*[Å¥ª®e+*ª®gkÅ+*¸ 2 Ê°Ã*h®{°gµ* 4 Ö`]-++j#À#®{hÔ]-* gµp½
(*h®{°ª®e"°Ã^(+*]°L?R­(+*:.JPH8²dÓÍ¹}'*h®{°Å¥¼ªº-´h®
{°YV 4 8><H'Ä´*1 ÐzÙ©Ë]Áa¿Â~®
2 ¨ 1 \ÎÙ\Î-j#À#~-( (r)1)

³o§h®{°fÃ-*

3 ¨ 2 \ÎÙª®¾&/R1DP-m)À#07MB<=\Î*
4 ÏÌwÙ\Îh®{°c¦(Ï¬!w*Å¥ÑX*ÇZ{-(*$ÉÇZ{£v5S37OT(®{¡-w¡
- 300 Æ}*++h®{°-¹')¨ 2 \Î'ÏÌw¡-¤83QUCT4-´h®{°ÏÌw¡*

ama1 £v swm1 £vAma1 NF2;TQ1U9¶o]* Anaphase Promoting Complex (APC) ®{¡¢µbk6GNC<@Swm1  APC 5TIUDT@*(gh®{°ÏÌw%¯*n¯­(+APC*º«h®{°c¦s*QT4Ã* Leading Edge Complex (LEC)y APC*¤·)h®{°ÏÌ APC _|¢ LEC Òl')´,++ÖÕh®{°¨ 2 \Î¡*Yh(º-´ gip1 £v×ÕØÕ

ispo71 £v spo73 £v-Spo71  Pleckstrin Homology AK.T- 2 Spo73  dysferlin AK.T-:TE3¾)+%®{u·*+((°\Î·*+(ÇZ{£v~x+*("ª®esº«+:TE3¾%h®{°s*"W®{°¤+¨ 2 \Îg{s»-´*tq1) Diamond A, Park JS, Inoue I, Tachikawa H, Neiman AM. (2009) Mol. Biol. Cell 20: 134-145
2) Tachikawa H, Bloecher A, Tatchell K, Neiman AM. (2001) J. Cell Biol. 155: 797-808
3) Ishihara M, Suda Y, Inoue I, Tanaka T, Takahashi T, Gao XD, Fukui Y, Ihara S, Neiman AM,Tachikawa H. (2009) Eukaryotic Cell 8: 1027-1037-.)%$*   #"+!',/#'/#"&(ø_¹í½~³Z/,½~âãMtQÄi2H9LHµãâäµV¶Ã°0MàPÐ0l,½~â)ZÈ¶µãp­0n&p­Ñï

mA45C3HFùBiofilmú
mA45C3HF*ò¤ÞÐ,>GDHµã¾-,'.%®ÞÐ,'.%
ã¦(SäÎÒ¿±­Éê-,

ÊµãÂMyg0,½~âåÝ×

v

*,Ò

*-,Òz-,*ÒmsõóÏÒmA45C3HF0Îk!âäÎÆm0Ód¯qÒmA45C3HF

,ã¦Sä¢¡Õf²0·ºÁ0Ð,
½~â'.%:JDHùmÛY¼Xßå)+]Oñú)+ã¦SäÎ0eëÒ
mA45C3HF0,Æm<7GL@J80Ð.SäÎ

ML11-11 (Lacbacillus plantarum) ã¦ Y11-43 (Saccharomyces cerevisiae) ÆmóÏTÎ¿ÒmA45C3HF0,0%ÒmA45C3HFÜrìwóçÔzFISH-`ª©IL;LóçÔzk!bê×öÇ
6Lg0Nã¦,

Çbê*(1)$bêk#h¬(ãÅc°ð0Ö*ã¦EJ?Jm£Ö0ÐÇEJ?JRæ

ØÙ,éO,ã¦bê§¥SäÎ0êÀsõ,SäÎîbêÌ«u´0j,
72J

SäÎ0Wbê,)+÷¤¡Ý[0ã¦ÑïEJ?JSäÎÑï=JB7è¸U\²»o-(2)h0²*SäÎ*e-^æ¡Ý0SäÎÑ}=JB»o-¤x 7 |K35 ¿K292 SäÎ0{ÚdÍã¦ÒmA45C3HF0

,Î0<7GL@J8. 3 SäÎ(L. plantarum 2  Leuconostocmesenteroides 1 )0%
0

$SäÎ ML11-11 ÒmA45C3HF

,ã¦<7GL@J80Ð.6 |K8 ¿K32 Q 30 ùa¨áã¦

14 úÒmsõA45C3HF'
`bêË0,*ML11-11
*

N

+"1ã¦¿ôã¦ÒmA45C3HF(1) Furukawa, S. et al.: Biosci. Biotechnol. Biochem., 74, 2316 (2010).
(2) Furukawa, S. et al.: Biosci. Biotechnol. Biochem., in submitting.

`bêËk!

-. /(!& 02' 1%3 4)$*"#
-.,+
0L>B-9Lîêéëµ·¿Ò0L>B-6;LFl*)0I2@GÉ*î£¿ðH;;LFÅh\{¥Û±(î­·¿Yx^Ì*)MÙ*8:=F)ï1960 Sd¦ÐÈ]*Táî0L>B-6;LF,ÜVw¼î0L>B-6;LF*)sî0L>B-6;LFÂª0L>B-9L¯²¾Ãk}Ðï

1990 S_dvã'ì¦ví4ILC&(î]ÄÝ¢ Saccharomyces cerevisiae,©M¼0L>B-9LOÀu«We,Ú$)N³°fï"
Atg8 iz*^w·¿¨¥y¬Ì0L>B-6;LFDL1L<JA3Ò*îGFP ,Åh Atg8 ,©¨·¿Ë|&(î0L>B-6;LF

*'£¿\^Ì*)#Û±,gÊc)î0L>B-6;LF£¿Õ[â*æpîgÀï]ÄÝ¢*)½'*)ï0L>B-6;LF 1 7/3I¶ 10 ^(î7/3I»(Ö*)

&(î0L>B-6;LF*£¿!×(Ô#*ï£¿Åh0L>B-6;LFt)# 1 ^"à,Ç)ï
0L>B-6;LFÂª¡*vmç)ï0L>B-6;LFå Atg <JA3ÒîÂÑØo<JA3Ò Atg9 M)î¦n+îAtg9 0L>B-6;LFÁÒ,X¹)Í'*ïÕî0L>B-6;LFÁÒ,X¹)0I2@GZÆî~¿WKE>5J?H.K·¿Âºrj~¿WÂ¥§¬º*ïb¥·¿îP0L>B-6;LFÂ*)wäwèß'*)

%(î¦nÁÒX¹¤a~¿W)ï]ÄÝ¢~¿W'ÂÓ×8:=F
0L>B-6;LFÞÇ`,)u«WÌ&(®'*)ïUÕÌ¸,qÏÎ,Ú$ïî0L>B-6;LFl*)ÁÒQÞÂ')0I2@G¨h^ÌÃk,%¯²,Ú$ï¯²''*®ÈR0I2@G^w,§Ì)N%v´½)ï

!" TOR #TOR 1;G7´¬§}Xx+6CFä5E0<F1;G7)â¥¿Ät®«qsc¿Är-^*b§ TOR H¹à8F=2ÐÀ¯*LÈgT TORC1 '*\ÇØ
Tor1p

TORC2 -]YÅ- 2  TOR 8F=2Ð Tor1p  Tor2p

SJ*â¥xnJ%
 TORC1 b§&[ÆØ¬-Ûv*Mei2 ¡cos¯(\TORC1 lwH* Mip1 -Mei2 ÁgÑ¯±¢\Ç-m*lwhz

"5@/F3DG> Tor2  Mei2+(-e\¬Ï^aTORC1¢\ÇA58G^lw Mei2 -²^*¶º{O_\ÇØ¬$*,\¬Ûv-*ÓIÔ TORC1 '*ÒZE?D§ª±³MU­-¸pk-*Mei2  Tor2 ÜÝV-ÌOJµÊ-

tor2 »¦s¯Ë°© TORC1 hâ¥dâÚ-WÕ*aâ¨'PÇØ¬&5^Þ£~ TORC1 ÖQw-j#¼¾¥áã°©Î|+*ÖQwuÇØ

TORC1 -TORC2 -*TORC1 rß*! TORC2 

:E5PJ¬ÃÉ+*tor2 £~eÅxnTor2p(TORC1 '*CFÙc¤+"in vitro .96/-­Tor2Mei2 8F=2Ð-Kyzc*Mei2 -²CFÙc*-·Í(9 ½hz++(CFÙc×R-.B<FÂs¯oMei2 8F=2Ðyzr"¬-Î|*Å`iIOI(TORC1  Mei2 -²8G49:CFÙc-N*fÅ(¬Ûv-^