29沼津工業高等専門学校研究報告
第 57 号，2023 年 2 月ᛴᛶ⫢⅖ࣔࢹ࣐ࣝ࢘ࢫ࡟࠾ࡅࡿ㤶↦Ⲕࡢᢠ㓟໬స⏝ⰾ㔝ᜤኈ *1࣭┿ቨຬ㑣 *1
Antioxidative Activity of Kosencha Extract on Mouse Model for Acute Hepatitis
YOSHINO Kyoji *1, MAKABE Isana *1
Abstract: Multiple health beneficial effects of tea ( Camellia sinensis) have been interested
worldwide. Kosencha is a secondary processed tea which is produced by treating Yabukita green
tea at high temperature and high pressure. Kosencha is characterized by less astringency andbitterness as compared to the original green tea. In this study, preventive effects of Kosencha
extract on mouse model for acute hepatitis were examined. Mouse hepatitis model was made byoral administration of carbon tetrachloride. Mice were given with 0.1%, 0.25%, and 0.5% (w/v)
aqueous solutions of the extracts of Kosencha and the original Yabukita green tea as a drinking
water 24 hr prior to the administration of carbon tetrachloride. Biochemical tests for mouseplasma, liver, and kidney were performed 24 hours after the administration of carbon
tetrachloride. The contents of liver triglyceride and total cholesterol, the levels of liver lipid
peroxide, and the activities of plasma glutamic oxaloacetic transaminase and glutamic pyruvic
transaminase were significantly increased in mice treated with carbon tetrachloride only ascompared to those in healthy mice, so severe fatty liver and hepatitis would be caused in miceadministered with carbon tetrachloride. Administration of the extracts of Kosencha or green tea
could not prevent these biochemical changes in hepatitis mice except for the case of liver lipid
peroxides. Intake of tea extracts significantly suppressed the elevation of liver lipid peroxidelevels in hepatitis mice in relation to the doses of water intake. No difference was observed
between the effects of the extracts of Kosencha and those of green tea. The antioxidative
components in Kosencha would be catechin monomers and their derivatives.
Key Words:

Kosencha, Tea, Hepatitis, Carbon tetrachloride, Antioxidative activity䠍䠊䛿䛨䜑䛻

ࡍࡿຠᯝࡢ࠶ࡿࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿ[5,6]ࠋ⫢⮚࡟ᑐࡋ࡚ᢠ㓟໬స⏝ࡢ࠶ࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿ⥳Ⲕ㸦Camellia sinensis㏆ᖺࠊ⫧‶ࡸ⏕ά⩦័⑓࡜㛵㐃ࡢ࠶ࡿ㠀࢔ࣝࢥ࣮ࣝᛶ⬡⫫var. sinensis㸧࠶ࡿ࠸ࡣࡑࡢ࢝ࢸ࢟ࣥᡂศ[7,8]ࡶࡲࡓࠊ⹫⾑ᛶ⫢⑌ᝈ㸦NAFLD㸧/㠀࢔ࣝࢥ࣮ࣝᛶ⬡⫫⫢⅖㸦NASH㸧ࡢ෌℺ὶ࡛ㄏᑟࡉࢀࡓ⬡⫫⫢࡜⫢㞀ᐖ[9]ࠊ⫧‶ࣔࢹ࣐ࣝ࢘ࢫ࡟᪥ᮏ࡟࠾ࡅࡿᝈ⪅ᩘࡢቑຍࡀ♫఍ၥ㢟࡜࡞ࡗ࡚࠸ࡿ[1]ࠋࡲࡓࠊ࠾ࡅࡿ⬡⫫⫢࡜⫢㞀ᐖ[10]ࠊ࢞ࣛࢡࢺࢧ࣑ࣥㄏⓎࡢ࢘࢖ࣝࢫ࢘࢖ࣝࢫឤᰁࡀཎᅉ࡜ࡉࢀࡿࡇ࡜ࡀከ࠸ᛴᛶ⫢୙඲ࡶὀどࡉᛶ⫢⅖ࣔࢹࣝࣛࢵࢺ࡟࠾ࡅࡿ⫢㞀ᐖ[11,12]࡞࡝ࠊᵝࠎ࡞⫢㞀ࢀ࡚࠸ࡿ[2]ࠋ⫢⅖࡟ࡣᵝࠎ࡞ື≀ࣔࢹࣝࡀ▱ࡽࢀ࡚࠸ࡿࡀࠊᐖࢆண㜵ࡍࡿຠᯝࡀሗ࿌ࡉࢀ࡚࠸ࡿࠋᅄሷ໬Ⅳ⣲࡟ࡼࡾᐇ㦂ⓗ࡟⫢◳ኚࢆ㉳ࡇࡉࡏࡿᡭἲࡣࡇࢀࡲ㤶↦Ⲕࡣࠊࣖࣈ࢟ࢱ᱂ᇵရ✀ࡢ⥳Ⲕࢆࡉࡽ࡟ຍᅽຍ⇕ฎ⌮࡛࡟ࡼࡃ⏝࠸ࡽࢀ࡚ࡁࡓ[3]ࠋᅄሷ໬Ⅳ⣲ࡣࠊࡑࡢ⫢⮚ቯṚ࡞ࡍࡿࡇ࡜࡛〇㐀ࡉࢀࠊ࢝ࢸ࢟ࣥ㢮ࡢἐ㣗Ꮚ㓟࢚ࢫࢸࣝࢆศゎ࡝ࡢ㔜⠜࡞ᛴᛶ㞀ᐖࡀ⫢ᑠ⬊యࡢࢳࢺࢡ࣮࣒ࣟ P-450 ⣔ࡢࣔࡋ࡚῰ࡳࢆ㍍ῶࡍࡿ࡜࡜ࡶ࡟㤶ࡤࡋࡉࢆ㧗ࡵࠊ㣧ࡳ᫆ࡉࢆྥ

ࣀ࢜࢟ࢩࢤࢼ࣮ࢮάᛶ࡟౫Ꮡࡋ࡚࠸ࡿࡇ࡜࠿ࡽࠊᑠ⬊య࡟ࡼୖࡉࡏࡓ஧ḟຍᕤⲔ࡛࠶ࡿ[13,14]ࠋᡃࠎࡣࠊ㤶↦Ⲕ࡟⥳Ⲕ࡜ࡗ࡚௦ㅰࡉࢀ࡚ࢺࣜࢡ࣓ࣟࣟࢳࣝࣛࢪ࢝ࣝࡀ⏕ᡂࡋࠊࡇࢀࡀ

ྠᵝ࡟ᢠ㓟໬స⏝ࡀ࠶ࡿࡇ࡜ࢆࠊin vitro ࡢ⣔[15]࠾ࡼࡧ⢾ᒀάᛶ㓟⣲ࡢ⏕ᡂࡸ⬡㉁㐣㓟໬཯ᛂࢆ㉳ࡇࡍࡓࡵ࡟⣽⬊⭷ࡢᦆ⑓ࣔࢹ࣐ࣝ࢘ࢫࡸ㧗⬡㣗ᦤྲྀ࣐࢘ࢫ࡟ࡼࡿ in vivo ࡢ⣔യࡸ⣽⬊Ṛࡀ㉳ࡇࡿࡶࡢ࡜⪃࠼ࡽࢀ࡚࠸ࡿ[4]ࠋᅄሷ໬Ⅳ⣲࡟

[16,17]࡛☜ㄆࡋ࡚࠸ࡿࠋࡲࡓࠊ࣋ࢽࣇ࢘࢟⥳Ⲕࡢ㤶↦Ⲕ࡟࣐ࡼࡿ⫢⅖௨እ࡛ࡶࠊ࠸ࡃࡘ࠿ࡢᢠ㓟໬ᛶ≀㉁࡟ࡘ࠸࡚ࠊ⬡㉁࢘ࢫ⓶⭵⅖࡟ᑐࡍࡿᢠ⅖⑕స⏝ࡀ࠶ࡿࡇ࡜ࡶሗ࿌ࡋ࡚࠸ࡿ㐣㓟໬཯ᛂࡸ⅖⑕࠿ࡽ⫢⣽⬊ࢆಖㆤࡋࠊ⫢⣽⬊ࡢቯṚࢆண㜵[18]ࠋࡑࡇ࡛ᮏ◊✲࡛ࡣࠊᅄሷ໬Ⅳ⣲ㄏⓎࡢᛴᛶ⫢⅖ࣔࢹ࣐ࣝ࢘ࢫ࡟࠾ࡅࡿࠊࣖࣈ࢟ࢱ⥳Ⲕ࠿ࡽ〇㐀ࡋࡓ㤶↦Ⲕࡢᢠ㓟໬*1 ≀㉁ᕤᏛ⛉Department of Chemistry & Biochemistryస⏝࡟ࡘ࠸᳨࡚ウࡋࠊཎᩱⲔࡢࡑࢀ࡜ẚ㍑ࡋࡓࠋ

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沼津工業高等専門学校研究報告䠎䠊ᮦᩱ䛚䜘䜃᪉ἲ

第 57 号ᐃ್㛫ࡢ┦㛵ᛶ࡟ࡘ࠸࡚ࡣࠊF-᳨ᐃࢆ⏝࠸᳨࡚ᐃࡋࡓࠋ
p<0.05 ࢆ⤫ィᏛⓗ࡟᭷ព࡛࠶ࡿ࡜ࡋࡓࠋ

㸰㸬㸯 Ⲕᢳฟ≀ࡢㄪ〇ࢧࣥࢲ࢖ࣖ♫ࡼࡾ౪୚ࡉࢀࡓࣖࣈ࢟ࢱ⥳Ⲕ࡜ࡑࡢ㤶↦Ⲕࡢ
Ⲕⴥ 8 g ࢆࠊ1 L ࡢἛ㦐Ỉ࡟ຍ࠼࡚ 10 ศ㛫ᢳฟࢆ⾜ࡗࡓࠋࡇ䠏䠊⤖ᯝ䛚䜘䜃⪃ᐹࢀࡽࡢᢳฟᾮࢆᐃᛶࢁ⣬ No.2 ࡛ࢁ㐣ࡋࠊ෾⤖஝⇱ࢆ⾜࠺ࡇ೺ᖖ⩌ࠊᅄሷ໬Ⅳ⣲ᢞ୚⩌ࠊ0.1㸪0.25㸪0.5%ࡢ⥳Ⲕᢳฟ≀࡜࡛ྛⲔࡢᢳฟ≀ࢆᚓࡓࠋ⥳Ⲕ࠾ࡼࡧ㤶↦Ⲕࡢᢳฟ≀ࡢⴥ࠿⩌ࡲࡓࡣ㤶↦Ⲕᢳฟ≀⩌ࡢ㣫⫱ࢹ࣮ࢱ࠾ࡼࡧ࣐࢘ࢫ⾑₢ࠊ⫢ࡽࡢ཰㔞ࡣࠊࡑࢀࡒࢀ 29.3%(w/w)࠾ࡼࡧ 30.5%(w/w)࡜࡯ࡰ

⮚࠾ࡼࡧ⭈⮚ࡢ⏕໬Ꮫⓗࢹ࣮ࢱࢆ⾲ 1 ࡟♧ࡍࠋ࣐࢘ࢫࡢ㣧Ỉᕪࡀぢࡽࢀ࡞࠿ࡗࡓࠋ

㔞ࡣࠊᖹᆒ࡛ 2.6 mL/1 ᪥/1 ༉࡛࠶ࡾࠊྛ⩌㛫࡟᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࡀࠊ೺ᖖ⩌ࡸᅄሷ໬Ⅳ⣲ᢞ୚⩌࡟ẚ㍑ࡋ࡚ࠊ

㸰㸬㸰 ᅄሷ໬Ⅳ⣲࠾ࡼࡧⲔᢳฟ≀ࡢ࣐࢘ࢫ࡬ࡢᢞ୚⥳Ⲕᢳฟ≀࠾ࡼࡧ㤶↦Ⲕᢳฟ≀ࡢ 0.5%⩌࡛ࡣ㣧Ỉ㔞ࡀపୗᖹᆒయ㔜 32.4 g ࡢ 4 㐌㱋 ddY ⣔㞝ᛶ࣐࢘ࢫࢆࠊ1 ⩌ 4 ༉ࡍࡿഴྥࡀ࠶ࡗࡓࠋࡇࡢཎᅉ࡜ࡋ࡚ࠊ࣐࢘ࢫࡀ᭱ࡶῧຍ㔞ࡢ

࡜ࡋ࡚ 8 ⩌࡟ศࡅࡓࠋ
೺ᖖ⩌࡟ࡣ㣧ᩱỈ࡜ࡋ࡚Ỉ㐨Ỉࢆ୚࠼ࠊከ࠿ࡗࡓⲔ㣧ᩱ࡜ᬑẁᦤྲྀࡋ࡚࠸ࡿỈ㐨Ỉ࡜ࡢ࿡ࡢ㐪࠸ࢆឤ

3 ᪥㛫㣫⫱ࡋࡓࠋᅄሷ໬Ⅳ⣲ᢞ୚⩌࡟ࡣ㣧ᩱỈ࡜ࡋ࡚Ỉ㐨Ỉࡌࡓྍ⬟ᛶࡀ⪃࠼ࡽࢀࡿࠋࢆ୚࠼ࠊ㣫⫱ 2 ᪥┠࡟ᅄሷ໬Ⅳ⣲㸸࣮࢜ࣜࣈἜΰᾮ㸦1:1㸧ࢆ ᅄሷ໬Ⅳ⣲ᢞ୚⩌࡛ࡣࠊ⫢⮚ࡢࢺࣜࢢࣜࢭࣜࢻ㔞ࠊ⥲ࢥࣞయ㔜 100 g ࠶ࡓࡾ 0.6 mL ࡜࡞ࡿࡼ࠺࡟⫶ࢰࣥࢹ࡛⤒ཱྀᢞ୚ࢫࢸ࣮ࣟࣝ㔞ࡀ೺ᖖ⩌࡟ẚ㍑ࡋ࡚᭷ព࡟ୖ᪼ࡋࠊ⬡⫫⫢ࡢᙧࡋࠊ3 ᪥┠ࡲ࡛㣫⫱ࡋࡓ[19]ࠋ⥳Ⲕᢳฟ≀ᢞ୚⩌࡜㤶↦Ⲕᢳฟᡂࡀ᥎ ࡉࢀࡓࠋ⫢⮚ࡢ㐣㓟໬⬡㉁ࣞ࣋ࣝࡶᅄሷ໬Ⅳ⣲ࡢᢞ≀ᢞ୚⩌࡛ࡣࠊࡑࢀࡒࢀࡢᢳฟ≀ࡢ 0.1%㸪0.25%㸪0.5% (w/v)୚࡛᭷ព࡟ୖ᪼ࡍࡿ࡜࡜ࡶ࡟ࠊ⫢ᶵ⬟ࡢᣦᶆ࡛࠶ࡿ⾑₢ GOTࡢỈ⁐ᾮࢆ㣧ᩱỈ࡜ࡋ࡚୚࠼ࠊ㣫⫱ 2 ᪥┠࡟⫢⅖⩌࡜ྠᵝ࡟άᛶࠊGPT άᛶࡢ᭷ព࡞ୖ᪼ࡶぢࡽࢀࠊ⫢㞀ᐖࡢⓎ⑕ࡀ☜ㄆᅄሷ໬Ⅳ⣲ࢆᢞ୚ࡋࡓᚋ 3 ᪥┠ࡲ࡛㣫⫱ࡋࡓࠋ㣫⫱ᮇ㛫୰ࠊࡉࢀࡓࠋ୍᪉ࠊ⫢⮚㔜㔞࡜⾑₢⥲ࢥࣞࢫࢸ࣮ࣟࣝ㔞ࡣࠊᅄሷ㣫ᩱࡣࡍ࡭࡚ࡢ⩌࡛࣐࢘ࢫ⏝ᬑ㏻㣗࡛࠶ࡿ PMI ♫〇⢊ᮎ㣫໬Ⅳ⣲ࡢᢞ୚࡟ࡼࡾ᭷ព࡟ῶᑡࡋࡓࠋࡑࢀ௨እࡢ⾑₢ࢺࣜࢢ

ᩱ LabDiet 4001 ࢆ୚࠼ࡓࠋᅄሷ໬Ⅳ⣲ᢞ୚ 24 ᫬㛫ᚋ࡟࣐࢘ࣜࢭࣜࢻ㔞ࠊྠ LDL-ࢥࣞࢫࢸ࣮ࣟࣝ㔞ࠊྠ㐣㓟໬⬡㉁ࣞ࣋

ࢫࢆᏳᴦṚࡉࡏࠊ⫢⮚࡜⭈⮚࠾ࡼࡧ⾑ᾮࢆ᥇ྲྀࡋࡓࠋ⫢⮚࡜ࣝࠊ⭈⮚㔜㔞ࠊྠ㐣㓟໬⬡㉁ࣞ࣋ࣝࡢ ᐃ್࡟ࡘ࠸࡚ࡣࠊᅄ⭈⮚࡟ࡘ࠸࡚ࡣࠊࡑࢀࡒࢀࡢ㔜㔞ࢆ ᐃࡋࡓᚋ࡟ࠊ⫢⮚ࡣ⏕

ሷ໬Ⅳ⣲ᢞ୚⩌࡜೺ᖖ⩌࡟᭷ព࡞ᕪࡣぢࡽࢀ࡞࠿ࡗࡓࠋ⌮㣗ሷỈ࡛℺ὶࡋࠊ⭈⮚ࡣ࿘ᅖࡢ⬡⫫ࢆྲྀࡾ㝖ࡁ⏕⌮㣗ሷỈ ᅄሷ໬Ⅳ⣲ࡢᢞ୚๓࠿ࡽ⥳Ⲕᢳฟ≀ࡲࡓࡣ㤶↦Ⲕᢳฟ≀ࢆ࡛Ὑίࡋࡓࠋ0.04M ࣜࣥ㓟⦆⾪ᾮ㸦pH 7.4㸧ࢆຍ࠼࡚ࠊࡑࢀ

ᦤྲྀࡋ࡚࠸ࡓ⥳Ⲕᢳฟ≀⩌࠾ࡼࡧ㤶↦Ⲕᢳฟ≀⩌ࡢ࣐࢘ࢫ࡛

ࡒࢀࡢ 20% (w/v)࣍ࣔࢪࢿ࣮ࢺࢆㄪ〇ࡋࠊ⫢⮚࡟ࡘ࠸࡚ࡣࢡ

ࡣࠊᅄሷ໬Ⅳ⣲ᢞ୚࡟ࡼࡿ⫢⮚ࡢࢺࣜࢢࣜࢭࣜࢻ㔞࠾ࡼࡧ⥲࣒ࣟࣟ࣍ࣝ:࣓ࢱࣀ࣮ࣝΰᾮ㸦2:1㸧࡛ᢳฟࡋࡓ⬡㉁୰ࡢࢺࣜࢢ

ࢥࣞࢫࢸ࣮ࣟࣝ㔞ࠊ⾑₢ࡢ GOT άᛶ࠾ࡼࡧ GPT άᛶࡢୖ᪼

ࣜࢭࣜࢻ㔞࡜⥲ࢥࣞࢫࢸ࣮ࣟࣝ㔞ࢆ࿴ග⣧⸆ᕤᴗ♫〇⮫ᗋ᳨ࡣᢚไࡉࢀ࡞࠿ࡗࡓࠋࡋ࠿ࡋࠊᅄሷ໬Ⅳ⣲ᢞ୚࡟ࡼࡿ⫢⮚ࡢᰝ࢟ࢵࢺࡢࢺࣜࢢࣜࢭࣛ࢖ࢻ E-ࢸࢫࢺ࣡ࢥ࣮࡜ࢥࣞࢫࢸࣟ㐣㓟໬⬡㉁ࣞ࣋ࣝࡢୖ᪼࡟ࡘ࠸࡚ࡣࠊ⥳Ⲕᢳฟ≀࠾ࡼࡧ㤶↦࣮ࣝ E-ࢸࢫࢺ࣡ࢥ࣮ࢆ⏝࠸࡚ ᐃࡋࡓࠋ⾑ᾮࡣࠊ᥇ྲྀᚋ㐲ᚰⲔᢳฟ≀ࡢ 0.1%࡜ 0.25%ࡢ⩌࡛ῧຍ㔞౫Ꮡⓗ࡟᭷ព࡟ᢚไ

ศ㞳㸦4Υ㸪 3000 rpm㸪 10 ศ㸧ࡋ࡚⾑₢ࢆᚓࡓࠋ⾑₢୰ࡢࡉࢀࡓࠋ࠸ࡎࢀࡢⲔࡢᢳฟ≀࡛ࡶ 0.5㸣ࡢ⩌࡛ࡣ㐣㓟໬⬡㉁ࢺࣜࢢࣜࢭࣜࢻ㔞࡜⥲ࢥࣞࢫࢸ࣮ࣟࣝࢆྠᵝࡢ⮫ᗋ᳨ᰝ࢟ࢵࣞ࣋ࣝࡢୖ᪼ࡣᢚไࡉࢀ࡚࠾ࡽࡎࠊࡇࢀࡽࡢ⩌࡛ࡣ௚ࡢῧຍࢺࢆ⏝࠸࡚ ᐃࡍࡿ࡜࡜ࡶ࡟ࠊపẚ㔜࣏ࣜࢱࣥࣃࢡ㉁㸦LDL㸧㔞ࡢ⩌࡟ẚ㍑ࡋ࡚࣐࢘ࢫ࡟ࡼࡿᦤྲྀࡀ༑ศ࡛࡞࠿ࡗࡓྍ⬟ᛶ-ࢥࣞࢫࢸ࣮ࣟࣝࡢ㔞ࢆྠ♫〇⮫ᗋ᳨ᰝ࢟ࢵࢺࡢ L ࢱ࢖ࣉ࣡ࡀ࠶ࡿࠋ࡞࠾ࠊ⥳Ⲕᢳฟ≀ࡸ㤶↦Ⲕᢳฟ≀ࡢ 0.5%⩌࡛ࠊ⫢⮚ࢥ࣮ LDL-C࣭M ࡜ࢥࣞࢫࢸ࣮ࣟࣝ E-ࢸࢫࢺ࣡ࢥ࣮ࢆ⏝࠸࡚㔜㔞࡜⭈⮚㔜㔞ࡀప࠿ࡗࡓࡇ࡜࡟ࡘ࠸࡚ࡣࠊࡇࢀࡽࡢ㔜㔞࡜ᐃࡋࡓࠋࡲࡓࠊ⾑₢୰ࡢࢢࣝࢱ࣑ࣥ㓟-࢜࢟ࢨࣟ㓑㓟ࢺࣛࣥᐇ㦂๓ࡢ࣐࢘ࢫࡢయ㔜ࡢ㛫࡟ᙅ࠸┦㛵ࡀࡳࡽࢀࡓࡇ࡜࠿ࡽࠊ

ࢫ࢔࣑ࢼ࣮ࢮ㸦GOT㸧࠾ࡼࡧࢢࣝࢱ࣑ࣥ㓟-ࣆࣝࣅࣥ㓟ࢺࣛࣥⲔᢳฟ≀ࡢᦤྲྀࡢᙳ㡪ࡔࡅ࡛࡞ࡃ࣐࢘ࢫࡢಶయᕪࡢ೫ࡾࡀཎ

ࢫ࢔࣑ࢼ࣮ࢮ㸦GPT㸧ࡢάᛶࢆࠊྠ♫〇⮫ᗋ᳨ᰝ࢟ࢵࢺࡢࢺ

ᅉ࡛࠶ࡿࡶࡢ࡜᥎ ࡉࢀࡿࠋࡑࡢ௚ࡢ ᐃ㡯┠࡛ࡣࠊ⥳Ⲕᢳࣛࣥࢫ࢔࣑ࢼ࣮ࢮ Cϩ-ࢸࢫࢺ࣡ࢥ࣮࡛ ᐃࡋࡓࠋ⾑₢࠾ࡼฟ≀⩌࠾ࡼࡧ㤶↦Ⲕᢳฟ≀⩌࡜ᅄሷ໬Ⅳ⣲ᢞ୚⩌ࡢ್࡟᭷ពࡧ⫢⮚࡜⭈⮚ࡢ 20%࣍ࣔࢪࢿ࣮ࢺ࡟ࡘ࠸࡚ࠊࡑࡢ㐣㓟໬⬡㉁࡞ᕪࡣぢࡽࢀ࡞࠿ࡗࡓࡀࠊ⾑₢ LDL-ࢥࣞࢫࢸ࣮ࣟࣝ㔞࡟ࡘࣞ࣋ࣝࢆࢳ࢜ࣂࣝࣅࢶ࣮ࣝ㓟ἲ࡟ࡼࡾ ᐃࡋࡓ[20,21]ࠋ

࠸࡚ࡣࠊⲔࡢᢳฟ≀⩌࡛ᅄሷ໬Ⅳ⣲ᢞ୚⩌࡟ẚ㍑ࡋ್࡚ࡀ㧗ᚓࡽࢀࡓᐇ㦂ࢹ࣮ࢱࡣࠊᖹᆒ್sᶆ‽೫ᕪ࡛⾲ࡋࡓࠋᐇ㦂ࡃ࡞ࡿഴྥࡀぢࡽࢀࡓࠋ⥳Ⲕ࢝ࢸ࢟ࣥࡢᦤྲྀ࡟ࡣࠊ⾑୰ LDL-⩌㛫ࡢᕪ࡟ࡘ࠸࡚ࡣࠊ୍ඖ㓄⨨ศᩓศᯒ㸦one-way ANOVA㸧ࢥࣞࢫࢸ࣮ࣟࣝ㔞ࡸ⫢⮚ࡢࢥࣞࢫࢸ࣮ࣟࣝ㔞ࢆపୗࡉࡏࡿຠ࡛ゎᯒࡋࠊḟ࠸࡛ Tukey ࡢከ㔜ẚ㍑ἲࢆ⏝࠸᳨࡚ᐃࡋࡓࠋྛᯝࡢ࠶ࡿࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿࡀࠊ⭠⟶࡛ࡢࢥࣞࢫࢸ࣮ࣟࣝ31

急性肝炎モデルマウスにおける香煎茶の抗酸化作用

⾲ 1 ᅄሷ໬Ⅳ⣲ㄏⓎ⫢⅖࣐࢘ࢫ࡟ᑐࡍࡿ㤶↦Ⲕྵ᭷㣧ᩱỈࡢᦤྲྀࡢᙳ㡪
೺ᖖ⩌

ᅄሷ໬Ⅳ⣲
ᢞ୚⩌
2.4 ± 1.4

0.1%
3.3 ± 0.1

ᅄሷ໬Ⅳ⣲ + ⥳Ⲕᢳฟ≀⩌
0.25%
0.5%
3.0 ± 0.3
1.9 ± 1.4

0.1%
2.7 ± 0.7

ᅄሷ໬Ⅳ⣲ + 㤶↦Ⲕᢳฟ≀⩌
0.25%
0.5%
2.8 ± 0.5
1.7 ± 0.9

2.6 ± 1.3
㣧Ỉ㔞㸦mL/᪥/࣐࢘ࢫ㸧⾑₢TG 㸦mg/dL㸧
92.0 ± 8.6
109.5 ± 23.4
108.7 ± 39.5
89.3 ± 13.3
100.0 ± 24.3
130.0 ± 8.2
108.5 ± 16.5
133.0 ± 27.8
ࠉT-Cho 㸦mg/dL㸧
75.6 ± 17.4*
54.3 ± 8.957.5 ± 15.7
56.6 ± 11.0
51.2 ± 8.2
51.1 ± 1.5
58.5 ± 5.1
61.5 ± 5.0
ࠉLDL-Cho 㸦mg/dL㸧
13.5 ± 7.3
14.6 ± 2.9
26.8 ± 2.7
27.0 ± 3.6
25.1 ± 1.7
23.9 ± 7.3
22.7 ± 4.1
22.7 ± 9.3
ࠉLPOx 㸦nmol/mL㸧
6.0 ± 0.2
5.5 ± 1.5
5.4 ± 0.2
5.4 ± 1.2
5.0 ± 1.0
4.4 ± 1.0
4.4 ± 1.1
4.8 ± 0.8
ࠉGOT 㸦IU/L㸧
20.4 ± 7.7**625.0 ± 16.9
644.9 ± 15.2
652.9 ± 16.6
646.9 ± 10.9
640.9 ± 8.7
629.4 ± 18.9
601.9 ± 5.1
ࠉGPT 㸦IU/L㸧
11.2 ± 3.7
209.8 ± 109.5
165.4 ± 35.8
197.0 ± 123.6 213.7 ± 114.0
254.9 ± 185.4
177.7 ± 87.0
234.8 ± 55.4⫢⮚
ࠉWT 㸦g㸧2.19 ± 0.18*
1.95 ± 0.09
1.85 ± 0.15
1.90 ± 0.15
1.50 ± 0.06**
1.95 ± 0.11
1.78 ± 0.15
1.58 ± 0.12**
ࠉTG 㸦mg/g㸧
37.0 ± 13.9*
74.6 ± 17.3
73.6 ± 25.4
76.1 ± 10.2
74.8 ± 15.0
83.9 ± 3.5
71.6 ± 18.5
84.7 ± 0.8
ࠉT-Cho 㸦mg/g㸧
4.3 ± 1.6**
10.2 ± 2.7
11.1 ± 2.1
9.3 ± 2.6
12.1 ± 1.8
11.9 ± 1.8
10.5 ± 1.4
11.4 ± 0.7
ࠉLPOx 㸦nmol/g㸧
61.8 ± 4.2**
249.7 ± 74.4
81.5 ± 8.1*
80.5 ± 26.7*
214.3 ± 96.5
90.3 ± 14.7*
70.7 ± 28.6**
245.4 ± 126.7⭈⮚
0.54 ± 0.05
0.57 ± 0.05
0.56 ± 0.09
0.49 ± 0.03
0.49 ± 0.01
0.59 ± 0.06
0.47 ± 0.05*
0.42 ± 0.04**
ࠉWT 㸦g㸧ࠉLPOx 㸦nmol/g㸧
102.3 ± 8.6
94.5 ± 21.3
86.2 ± 20.7
111.8 ± 11.7
107.9 ± 21.3
104.1 ± 16.1
114.8 ± 5.4
111.4 ± 20.6
ࠉ TG, ࢺࣜࢢࣜࢭࣜࢻ㔞; T-Cho, ⥲ࢥࣞࢫࢸ࣮ࣟࣝ㔞; LDL-Cho, పẚ㔜࣏ࣜࢱࣥࣃࢡ㉁-ࢥࣞࢫࢸ࣮ࣟࣝ㔞; LPOx, 㐣㓟໬⬡㉁ࣞ࣋ࣝ; GOT, ࢢࣝࢱ࣑ࣥ㓟-࢜࢟ࢨࣟ㓑㓟ࠉ ࢺࣛࣥࢫ࢔࣑ࢼ࣮ࢮάᛶ; GPT, ࢢࣝࢱ࣑ࣥ㓟-ࣆࣝࣅࣥ㓟ࢺࣛࣥࢫ࢔࣑ࢼ࣮ࢮάᛶ; WT, 㔜㔞㸬ࠉ࣐࢘ࢫࡣྛ⩌4༉㸬࣐࢘ࢫ࡟Ⲕᢳฟ≀ྵ᭷㣧ᩱỈࢆ⮬⏤ᦤྲྀࡉࡏ࡚24᫬㛫ᚋ࡟ᅄሷ໬Ⅳ⣲ࢆᢞ୚ࡋ㸪ࡉࡽ࡟24᫬㛫ᚋ࡟⏕໬Ꮫⓗ᳨ᰝࢆ⾜ࡗࡓ㸬ࠉᅄሷ໬Ⅳ⣲ᢞ୚⩌࡜ࡢ⤫ィᏛⓗ᭷ពᕪ㸹 * p<0.05, ** p<0.01㸬ᐃ್㛫䛾᭷ព䛺ṇ䛾┦㛵䠖 p<0.01; ⾑₢GOT䠉⾑₢GPT, ⾑₢GOT䠉⫢⮚TG, ⾑₢GOT䠉⫢⮚T-Cho, ⾑₢GPT䠉⫢⮚TG, ⾑₢GPT䠉⫢⮚T-Cho, ⫢⮚TG䠉⫢⮚T-Cho䠊ᐃ್㛫䛾᭷ព䛺㈇䛾┦㛵䠖 p<0.05; 㣧Ỉ㔞䠉⫢⮚LPOx, ⾑₢LPOx䠉⫢⮚T-Cho, ⫢⮚WT䠉⫢⮚T-Cho, ⭈⮚WT䠉⭈⮚LPO䠗 p<0.01; ⾑₢T-Cho䠉⾑₢GOT,⾑₢T-Cho䠉⾑₢GPT, ⾑₢T-Cho䠉⫢⮚TG, ⾑₢T-Cho䠉⫢⮚T-Cho䠊ࡢ྾཰ࡢᢚไ[22]ࠊ⫹Ồࢥࣞࢫࢸ࣮ࣟࣝࡢศἪࡢಁ㐍[23]ࠊ⫢࡜⥳Ⲕ࡟ẚ㍑ࡋ࡚༙ῶࡋ࡚࠸ࡿࠋ㤶↦Ⲕࡢຍᕤ࡟ࡼࡾἐ㣗Ꮚ⣽⬊࠿ࡽࡢ࢔࣏࣏ࣜࢱࣥࣃࢡ㉁ B ࡢศἪࡢᢚไ[24]࠶ࡿ࠸ࡣ

㓟ࡣ 0.02%(w/w)࠿ࡽ 0.11%(w/w)࡟ቑຍࡋ࡚࠾ࡾࠊ࢝ࢸ࢟ࣥ

⫢⣽⬊ࡢ LDL ཷᐜయࡢⓎ⌧ୖ᪼[25]࡞࡝ࠊࡑࡢస⏝ᶵᗎࡣ」ࡢἐ㣗Ꮚ㓟࢚ࢫࢸࣝࡢศゎ࡟ࡼࡾ῰ࡳࡀ㍍ῶࡋ࡚࠸ࡿ࡜⪃࠼ᩘ࠶ࡿࡇ࡜ࡀ⪃࠼ࡽࢀ」㞧࡛࠶ࡿࠋᮏᐇ㦂࡛ࡣࠊⲔࡢᢳฟ≀ࡽࢀࡿࡀࠊቑຍࡋࡓἐ㣗Ꮚ㓟ࡢ㔞ࡣ㤶↦Ⲕ୰ࡢ࢝ࢸ࢟ࣥ༢㔞ࡢᙳ㡪ࡔࡅ࡛࡞ࡃࠊᅄሷ໬Ⅳ⣲ࡢ⫢㞀ᐖ࡟ࡼࡿ⾑୰⬡㉁࡜⫢య௨እࡢ࣏ࣜࣇ࢙ࣀ࣮ࣝࡢ㔞࡟ᙜࡓࡿ3.89%(w/w)ࡢ 3%⛬ᗘ⮚⬡㉁ࡢኚືࡀ㛵ࢃࡗ࡚࠸ࡿࡓࡵࠊ⾑୰ LDL-ࢥࣞࢫࢸ࣮࡛ࣟࡋ࠿࡞࠸ࠋᚑࡗ࡚ࠊ㤶↦Ⲕ࡟ࡣ࢝ࢸ࢟ࣥ༢㔞య࠾ࡼࡧἐ㣗್ࣝࡀⲔᢳฟ≀⩌࡛㧗࠸ഴྥࡀぢࡽࢀࡓᶵᵓࢆ⌮ゎࡍࡿࡇ࡜Ꮚ㓟௨እࡢ࣏ࣜࣇ࢙ࣀ࣮ࣝࡀ⣙ 46%ྵࡲࢀ࡚࠸ࡿࡇ࡜࡟࡞ࡣ㞴ࡋ࠸ࡶࡢ࡜⪃࠼ࡽࢀࡿࠋࡿࠋ㤶↦Ⲕ࡟⣙༙㔞ࡀṧᏑࡍࡿ࢝ࢸ࢟ࣥ༢㔞యࡣࠊ㤶↦Ⲕࡢ ྛ ᐃ್ࡢ㛫ࡢ┦㛵㛵ಀ࠿ࡽࡣࠊᅄሷ໬Ⅳ⣲ࡢᢞ୚࡟ࡼࡾᢠ㓟໬స⏝࡟ᐤ୚ࡋ࡚࠸ࡿࡶࡢ࡜⪃࠼ࡽࢀࡿࡀࠊ⥳Ⲕ࡜㤶↦࣐࢘ࢫࡢ⫢⮚࡛㓟໬ࢫࢺࣞࢫࡀ㧗ࡲࡾ⬡㉁ࡀ㓟໬ࡉࢀࡓࡇ࡜Ⲕࡢᢠ㓟໬స⏝ࡀྠ⛬ᗘ࡛࠶ࡿࡇ࡜࠿ࡽࠊ࢝ࢸ࢟ࣥ༢㔞య௨࡛ࠊ⾑୰ࡢࢺࣜࢢࣜࢭࣜࢻࡸࢥࣞࢫࢸ࣮ࣟࣝ࡜࠸ࡗࡓ⬡㉁ࡀ

እࡢ࣏ࣜࣇ࢙ࣀ࣮ࣝࡶࡑࡢస⏝࡟㛵୚ࡋ࡚࠸ࡿࡶࡢ࡜ண᝿ࡉ⫢⮚࡟⿵ሸࡉࢀ࡚⬡⫫⫢ࡀᙧᡂࡉࢀࠊ⵳✚ࡉࢀࡓ⬡㉁ࡀࡉࡽ

ࢀࡿࠋ஧ḟຍᕤⲔࡢ୍ࡘ࡛ࠊ⥳Ⲕࢆຍ⇕ฎ⌮ࡍࡿࡇ࡜࡛〇㐀࡟㓟໬ࡉࢀ࡚⣽⬊㞀ᐖࡀ㉳ࡁࡓࡇ࡜࡛⾑୰ࡢ⫢ᶵ⬟࣐࣮࣮࢝ࡉࢀࡿ࡯࠺ࡌⲔ࡟࢝ࢸ࢟ࣥࡢ㔜ྜ≀ࡸศゎ≀ࡀྵࡲࢀࡿ࡜⪃࡛࠶ࡿ GOT άᛶࡸ GPT άᛶࡀୖ᪼ࡋࡓࡇ࡜ࡀ᥎ ࡉࢀࡿࠋ࠼ࡽࢀࡿࡇ࡜࠿ࡽ[26,27]ࠊ㤶↦Ⲕ࡟ࡶྠᵝࡢᡂศࡀྵࡲࢀࡿ⥳Ⲕᢳฟ≀࡜㤶↦Ⲕᢳฟ≀ࡢᦤྲྀࡣࠊᅄሷ໬Ⅳ⣲࡟ࡼࡿ࣐࢘ࡶࡢ࡜ண᝿ࡋ࡚࠸ࡿࠋ௒ᚋࠊࡑࡢ᭷ຠᡂศ࡟ࡘ࠸᳨࡚ウࡍࡿࢫ⫢⮚୰ࡢ㓟໬ࢫࢺࣞࢫࢆ࠶ࡿ⛬ᗘᢚไࡍࡿᶵ⬟ࡣⓎ᥹ࡋࡓᚲせࡀ࠶ࡿࠋࡶࡢࡢࠊ௒ᅇࡢᐇ㦂࡛ࡣᅄሷ໬Ⅳ⣲࡟ࡼࡿ⬡⫫⫢࡜⫢㞀ᐖࡢ⛬ᗘࡀᙉ࠿ࡗࡓࡓࡵࠊ⫢⮚ࡢ⅖⑕ࢆ༑ศ࡟ᢚไࡍࡿࡇ࡜ࡲ࡛ࡣ࡛ࡁ࡞࠿ࡗࡓࡶࡢ࡜⪃࠼ࡽࢀࡿࠋ࡞࠾ࠊ࣐࢘ࢫࡢ㣧Ỉ㔞࡜䠐䠊䜎䛸䜑⫢⮚ࡢ㐣㓟໬⬡㉁ࣞ࣋ࣝ࡜ࡢ㛫࡟ࡣ㈇ࡢ᭷ព࡞┦㛵ᛶࡀぢࡽᮏᐇ㦂࡛ࡣࠊ࣐࢘ࢫ࡟ᅄሷ໬Ⅳ⣲ࢆᢞ୚ࡋ࡚⫢㞀ᐖࢆ㉳ࡇࢀࠊⲔࡢᢳฟ≀ࡢᦤྲྀ㔞࡜⫢⮚࡛ࡢ㓟໬ࢫࢺࣞࢫᢚไస⏝࡟ࡉࡏࡓ㝿ࡢࠊࣖࣈ࢟ࢱ⥳Ⲕ࡜ࡑࡢ㤶↦Ⲕࡢᢳฟ≀ࢆῧຍࡋࡓࡣ୍ᐃࡢ㛵ಀࡀ࠶ࡗࡓࡶࡢ࡜⪃࠼ࡽࢀࡿࠋ㣧ᩱỈࡢ⮬⏤ᦤྲྀࡢᙳ㡪ࢆ᳨ウࡋࡓࠋᅄሷ໬Ⅳ⣲ࡢᢞ୚࡛Ⓨ⥳Ⲕࡢᢳฟ≀࡟ྵࡲࢀࡿᢠ㓟໬ᡂศ࡜ࡋ࡚ࡣࠊከ㔞࡟ྵࡲ⑕ࡋࡓ⬡⫫⫢࡜⫢㞀ᐖࡣࠊ࠸ࡎࢀࡢⲔࡢᢳฟ≀ࡢᦤྲྀ࡟ࡼࡗࢀࡿ࢝ࢸ࢟ࣥ༢㔞యࡀ⪃࠼ࡽࢀࡿࠋ㤶↦Ⲕࡢཎᩱ⥳Ⲕⴥ୰ࡢ࡚ࡶᨵၿࡉࢀ࡞࠿ࡗࡓࠋࡋ࠿ࡋࠊ0.1%࠶ࡿ࠸ࡣ 0.25%ࡢྛⲔ

࢝ࢸ࢟ࣥ༢㔞యࡢ⥲㔞ࡣ 7.99% (w/w)࡜ࠊࡑࡢ⥲࣏ࣜࣇ࢙ࣀ

ᢳฟ≀ࢆྵࡴ㣧ᩱỈࢆᦤྲྀࡋࡓ࣐࢘ࢫ࡛ࡣࠊ⫢⮚ࡢ㐣㓟໬⬡࣮ࣝ㔞 9.28%(w/w)ࡢ⣙ 86%ࢆ༨ࡵࡿ[14]ࠋ୍᪉ࠊ㤶↦Ⲕⴥ୰㉁ࣞ࣋ࣝࡢୖ᪼ᢚไຠᯝࡀぢࡽࢀࡓࠋ⥳Ⲕ࡜㤶↦Ⲕ࡛ᮏຠᯝ

ࡢ⥲࣏ࣜࣇ࢙ࣀ࣮ࣝ㔞ࡣ 8.29%(w/w)࡜ཎᩱ⥳Ⲕ୰ࡢ㔞ࡢ⣙࡟ᕪࡣぢࡽࢀࡎࠊ῰ࡳࢆᢚ࠼ࡓ㤶↦Ⲕࡣࡼࡾᦤྲྀࡋࡸࡍ࠸㣧89%ࢆಖᣢࡋ࡚࠸ࡿࡀࠊ
࢝ࢸ࢟ࣥ༢㔞యࡢ⥲㔞ࡣ 4.40%(w/w)ᩱ࡜⪃࠼ࡽࢀࡿࠋ௒ᅇࡢᐇ㦂࡛ࡣⲔࡢᢳฟ≀ࡢᦤྲྀ࡟ࡼࡿ⫢㞀ᐖࡢᝏ໬ࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࡀࠊ⥳Ⲕࢧࣉ࣓ࣜࣥࢺࡢᦤྲྀ

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沼津工業高等専門学校研究報告

第 57 号ࡀ⫢㞀ᐖ࡜㛵ಀࡍࡿ࡜⪃࠼ࡽࢀࡿ⑕౛ࡢሗ࿌ࡶ࠶ࡿࡇ࡜࠿ࡽ

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