MicroTime 200
A versatile platform for time-resolved confocalmicroscopy•••••••••

Complete confocal system with laser combining unit, inverted microscope bodyTurn-key diode lasers for multicolor excitation from 375 to 900 nm
Up to 6 truly parallel detection channels using application-optimized detection with SPADs, PMTs or Hybrid-PMTs
Time-Correlated-Single Photon Counting (TCSPC) and TTTR modes
for investigatig fast dynamics with FCS and FLIM
Two optional exit ports for additional hardware like spectrographs
SymPhoTime 64: advanced easy-to-use software for data acquisition, analysis, and visualization
Unique upgrades: 2focus FCS, simultaneous AFM/FLIM, deep UV excitation
STED add-on for super-resolution imaging
Scanning options:
FLIMbee galvo scanner add-on for outstanding flexibility in scanning speed with excellent spatial accuracy
or piezo scanning for ultra precise point positioning and optical distortion free imaging
NEW FLIMbee linescan modus in x, allowing scanning FCSApplications•••••••••••••

Single Molecule Spectroscopy/Detection
Fluorescence Lifetime Imaging (FLIM)
Phosphorescence Lifetime Imaging (PLIM)
Fluorescence Correlation Spectroscopy (FCS)
Fluorescence Lifetime Correlation Spectroscopy (FLCS)
Foerster Resonance Energy Transfer (FRET)
Dual-focus Fluorescence Correlation Spectroscopy (2fFCS)
NEW scanning FCS (sFCS)
Pulsed Interleaved Excitation (PIE)
Fluorescence Anisotropy (Polarization)
Pattern Matching Analysis
Time-Resolved Photoluminescence (TRPL)
Antibunching

Scientific advances at the cutting edge of many fields hinges on single molecule studies. This includes, for example, thequantification of molecular dynamics or molecular properties as well as interaction studies in material and life sciences.
Such a wide field of research requires a flexible instrument, which can be adapted to the individual needs. This versatility isgiven in the MicroTime 200, a time-resolved confocal fluorescence microscope system. This powerful instrument is readyto analyze a multitude of parameters down to the single molecule level using methods such as Fluorescence Lifetime Imaging (FLIM), FLIM/FRET, deep tissue FLIM, PIE, FCS/FCCS, FLCS/FLCCS, dual-focus FCS, anisotropy, burst analysis,
simultaneous AFM/FLIM or deep UV detection, to name only the most common. Even high resolution imaging with spatial
resolutions below 50 nm is possible with the new MicroTime 200 STED add-on.

Specifications
Objectives*Standard

PL 20x PlanAchromat, NA 0.4, air spaced, 400-750 nm
PL 40x PlanAchromat, NA 0.65, air spaced, 400-750 nm

Optional

UPLSAPO 60x PlanApochromat, NA 1.2, water immersion, 360-900 nm
UPLSAPO 100x PlanApochromat, NA 1.4, oil immersion, 370-850 nm
UAPON 100x Apochromat, NA 1.49, oil immersion, 370-850 nm
other oil immersion, apochromatic correction, air spaced, IR/UV-enhanced orlong working distance, TIRF objectives

Detectors
Type*SPAD(PDM Series)

PMA Hybrid- 40

PMA 175

SPAD(SPCM-AQRH)

Spectral range

400 - 1 000 nm

300 - 720 nm

230 - 700 nm

400 - 1 000 nm

Dark counts (at 20 °C, typ. value)< 250 cps

< 700 cps

< 50 cps< 100 cps

Photon detection efficiency

50 % at 550 nm

45 % at 500 nm> 40% @ 400 nm> 70 % at 700 nm

Excitation system
Picosecond diode lasers (375 - 900 nm) with repetition rates up to 80 MHz inside
a compact Laser Combining Unit
Single or multichannel laser driver
Optional: excitation down to 266 nm
Optional: integration of external laser (e.g., Titanium:Sapphire laser)
For STED

640 nm (excitation)
765 nm (STED laser)
triple species STED with 595, 640 and 660 nm excitation
Easy-STED phase plate for alignment-free STED imaging

Scanning
Comprehensive software control via SymPhoTime 64
Fast galvo scanner (FLIMbee) with highly flexible scanning speeds (mandatoryfor rapidFLIM, highly recommended for STED, not suitable for UV), see ourFLIMbee brochure for details
Optical distortion free 3-dimensional piezo objective scanning with 80 x 80 x 80
μm scan range at nominal 1 nm positioning accuracy, mandatory for deep UV
imaging
Available in combination with z stage for 3D imaging
Data acquisition
TypeHydraHarp 400

TimeHarp 260
PICO

MultiHarp150
4PTime resolution (bin width)

1 ps

25 ps

10 ps

Dead time< 80 ns< 25 ns0.65 ns

Operating environment
Power requirements

110 to 230 V, 50/60 Hz
Power consumption of a standard system: 6 A @ 230V

Dimensions (w × d × h)
Laser combining unit

600 × 400 × 200 mm (without laser driver)

Microscope and main optical unit

1150 × 600 mm (2 detection channels)

19’’ electronic rack (typ.)600 × 550 × 900 mm

Table

Optical table with imperial or metric breadboard required.
We highly recommend active vibration isolation.
The table size depends on system options, please ask us for our detailed pre-installation requirements.

* Other types are available upon request.

PicoQuant GmbH
Rudower Chaussee 29 (IGZ)
12489 Berlin
Germany

Phone
Telefax
EmailWeb+49-(0)30-1208820-0+49-(0)30-1208820-90
info@picoquant.com
www.picoquant.com

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are
subject to change without notice. Trademarks or corporate names are used for explanation and identification, to the owner’s benefit and without intent to infringe.
© PicoQuant GmbH, August 2020