北海道大学

大学院農学院

修士論文発表会，2017 年 2 月 1 日〜13 日

Effects of chemical fertilizer and manure application on dissolved
carbon leaching and carbon budget in a managed grassland insouthern Hokkaido, Japan

Division of Environmental Resources, Chair of Regional Environment, Soil Science, Fu Yang
1. Introduction
Grassland ecosystem comprises approximately 40.5% of the earth’s terrestrial land area,
which plays an important role in carbon (C) balance at the global scale. Export of dissolved
organic carbon (DOC) and dissolved inorganic carbon (DIC) from grassland ecosystems canbe an important C flux which directly affects ecosystem C balance after leaching from the soil
to the groundwater and causes contamination. However, few studies have documented Cleaching and the C balance including C leaching loss. Fertilizer and manure, as soil
amendments, are widely used nowadays to increase productivity and enhance soil fertility inagriculture. This study aims to investigate the effects of chemical fertilizer and manure
application on dissolved C leaching and estimate C budget considering C leaching losses.
2. Materials and Methods
A field experiment was conducted in a managed grassland with Timothy (Phleum pretense L.)of Shizunai livestock farm (42°26'N, 142°29'E) in Southern Hokkaido, Japan, from June 2014
to May 2016. Three treatments, a control (CT), chemical fertilizer (F) and manure (4192.9 kg
C ha-1yr-1) and chemical fertilizer (MF) were established at the begining of June 2014 withfour replicates. Tension free lysimeters (0.05 m2 area, 1 cm height of collars on 3 sides) wereinstalled at 30cm depth below ground to monitor DOC and DIC leaching. Amounts of DOC
and DIC leaching were estimated by the DOC and DIC concentrations of the percolated waterand the water fluxes calculated by water balance method. Net primary production (NPP) wasestimated using harvest method. Heterotrophic respiration (RH) was measured by closed
chamber method. Water and gas samples were taken every one or two weeks in growing
season and three or four weeks in non-growing season. The net biome production (NBP) wasestimated as NPP – RH – Harvest + Manure +Lime – DOC – DIC.
3. Results and discussion
1) Both dissolved C concentration in percolate water and dissolved C leaching loss weresignificantly higher in MF plot than in CT and F plots (p<0.01), indicating that manure
application could increase C leaching loss through increasing organic C source in soil.
2) The amount of SOC in 0-30cm soil depth was 103.2 and 129.8 Mg C ha-1yr-1in F and MF
plots, respectively. And C from manure was 4.2 Mg C ha-1yr-1. Results showed that 98.9 kg C

ha-1yr-1 C of manure and 69.2 kg C ha-1yr-1 C of SOC were leached out from top soil. 1051kg C
ha-1yr-1 C of manure and 4996.7 kg C ha-1yr-1 C of SOC were emitted from the surface soil. The
sum of leached and emitted C accounted for 27.4% and 4.9% of manure C and SOC, respectively.

3) The NBP was negative in all treatments. However, MF showed significantly highest(p<0.01) among the treatments (-1.3 ± 1.0 Mg C ha-1yr-1), followed by CT (-3.6 ± 1.1 Mg C
ha-1yr-1) and significantly lowest (p<0.01) in F (-4.2 ± 1.5 Mg C ha-1yr-1), indicating thatmanure application could mitigate C loss in grassland ecosystem. Although chemical fertilizer
can increase productivity, it could increase the net C loss.
4) Total dissolved C leaching contributed to 2.2%, 1.6% and 12.9% of NBP in CT, F and MF
plots, respectively. This reveals that it is necessary to take C leaching loss into consideration
when estimating C budget because of its non-negligible fractions to NBP.
4. Conclusions
1) Manure application significantly increased C leaching losses by increasing organic C
source in soil (p<0.01). While chemical fertilizer had no significant effects on C leaching loss.
2) Both chemical fertilizer and manure application led to net C loss in this grassland
ecosystem, however, manure addition could significantly mitigate C loss (p<0.01).
3) Due to the non-negligible contribution of C leaching loss to NBP, it is important to take C
leaching into consideration to develop reliable estimates of net ecosystem C balances.