RESEARCH PRODUCTS

Cambridge Isotope Laboratories, Inc.
isotope.com

Metabolomics
QReSS™ Kits
For Untargeted and Targeted
Mass Spectrometric Analysis
To ensure high-quality metabolomics results, the method and instrument system must be qualified as being fit for purpose. This involves
testing for losses or errors in the analytical workflow. To aid such performance assessments in MS metabolomics and enable
metabolite quantification, Cambridge Isotope Laboratories, Inc. (CIL) is pleased to offer the QReSS™ (Quantification, Retention,and System Suitability) kit. Its potential use could also extend to other applications, such as metabolite retention indexing.
The kit contains the following materials and tools:•	2 Vials of stable isotope-labeled metabolite mixes (lyophilized)•	User manual (with example procedures and LC-MS methods)
Catalog No.
MSK-QReSS-KITMSK-QReSS1-1
MSK-QReSS2-1

Description
Metabolomics QReSS Kit
Metabolomics QReSS Standard Mix 1
Metabolomics QReSS Standard Mix 2

Note: Unlabeled mixes are also available. Please inquire.

This kit, through collaboration with Sciex, was validated in differentmatrices (e.g., plasma, urine, CHO cells) using a single injection,
high-flow UHPLC-MRM/MS method (QTRAP ® 6500+). Notethat the mixes can also be extended to alternate LC-MS platforms.
Procedurally, after reconstituting and mixing the kit vials, a working
aliquot can be applied in various ways for use in metabolomic
LC-MS/MS exercises. This enables the analytical performance
to be evaluated and quantitative determinations of metabolites
to be made (see app note #49 for example overview).

Table. Mix compositions. Reconstituting each vial in 1 mL of solvent (e.g., 50% methanol) will yield the specified concentrations.
Description
L-Alanine (13C3, 99%; 15N, 99%)
1,4-Butanediamine (putrescine)·2HCl (13C4, 99%)
Creatinine (N-methyl-D3, 98%)
Ethanolamine·HCl (1,1,2,2-D4, 98%)
Guanosine·2H2O (15N5, 96-98%)
Hypoxanthine (13C5, 99%)
L-Leucine (13C6, 99%)
L-Phenylalanine (ring-13C6, 99%)
Thymine (1,3-15N2, 98%)
L-Tryptophan (13C11, 99%)
L-Tyrosine (ring-13C6, 99%)
Vitamin B3 (nicotinamide) (13C6, 99%)
Citric acid (1,5,6-carboxyl-13C3, 99%)
Fumaric acid (13C4, 99%)
Indole-3-acetic acid (phenyl-13C6, 99%)
α-Ketoglutaric acid, disodium salt (1,2,3,4-13C4, 99%) CP 97%
Sodium palmitate (U-13C16, 98%)
Sodium pyruvate (13C3, 99%)
Please inquire for pricing and companion unlabeled mixtures.
Chemical purity (CP) is 98% or greater, unless otherwise indicated.
For research use only. Not for use in diagnostic procedures.

Chemical Formula
13
C3H715NO2
13
C4H12N2·2HCl
C4H4D3N3O
C2H3D4NO·HCl
C10H1315N5O513
C5H4N4O
13C6H13NO213C6C3H11NO2
C5H615N2O213C11H12N2O213
C6C3H11NO313C6H6N2O
13C3C3H8O713C4H4O413C6C4H9NO213C4CH4Na2O513C16H31O2Na
13
C3H3O3Na

MW (Da)93.07
92.10
116.1465.11
288.21141.08137.13
171.15128.10215.14187.14128.08195.10120.04181.14194.03294.29113.02

Conc. (µg/mL)
100
10100
102
10
5100
20100
100
510100
5100
10100

Vial
111111111111222222

Application NotePercy, A.J.; Proos, R.; Demianova, Z.; Backiel, K.; Ubhi, B.K. 2021. Standardizing
quantitative metabolomics analyses through the QReSS™ kit. (CIL note #49)

Cambridge Isotope Laboratories, Inc.
North America: 1.800.322.1174 cilsales@isotope.com | International: +1.978.749.8000 intlsales@isotope.com | fax: 1.978.749.2768 | isotope.com
MET_RSCH_QReSS (6/17/21) Supersedes all previously published literature