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of Cell Growth Media.

Stable Isotope
Cell Growth Media
Structural studies on proteins using nuclear magnetic resonance (NMR) require labelling of these moleculeswith stable isotopes. Organisms such as bacteria and yeast are used to express the proteins. Silantes offers
a wide range of stable isotope-labelled cell growth media to express stable isotope-labelled proteins.

Available in Multiple Variations, Tailored to Your Needs
Depending on your application and purpose, we offer three different media variations for the expressionof stable isotope-labelled proteins: (Please see next page for a more detailed description)-

The High Performance OD2 Rich Growth Media solution,-The SILEX Powder Media and-The ECO Powder Media.

All three variations are available optimized for the growth of E.coli or yeast in standard pack sizes (100 mL,200 mL, 500 mL, 1 L for media solution, 1 g, 10 g, 100 g for powder media) or in bulk sizes.

High Standards in Quality Control
The media are available in all combinations of the isotopic labels 2H, 13C, 15N with an isotopic enrichment of> 98 %. This isotopic enrichment is validated by mass spectrometry. Biological competence is validated ingrowth tests. The results of the quality control tests are included in each delivery.

No Additional 13C-Glucose Needed
All Silantes cell growth media are prepared from uniformly labelled bacterial cell hydrolysate of Ralstonia

eutropha. If the media are used as stand-alone media, no additional 13C-glucose is needed in order toexpress 13C-labelled proteins.

Request for a free Media sample at sales@silantes.com!
Silantes also offers stable isotope labelled reagents for M9 media such as 13C-glucose, D2O and 15NH4Cl.
Have a look at the currently available Silantes products and services at www.silantes-shop.com.

www.silantes.com|

www.silantes-shop.com|0049 (0)89 500 941 0|

info@silantes.com

Scan the QR-Code to see our product catalogue
of Cell Growth Media.

Stable Isotope
Cell Growth MediaHigh Performance OD2 Rich Growth MediaThe Silantes OD2 medium is a ready-to-use rich growth medium solution for highperformance expression of stable isotope labelled proteins. The media are optimized
for cell growth and expression of homologous and heterologous proteins.
Our OD2 media are made from labelled bacterial hydrolysate and contain primarily
amino acids, some low molecular weight oligopeptides and almost no carbohydrates.
The bacterial strain used is a chemolithoautotrophic organism grown on isotopicallylabelled inorganic substrates.

SILEX Powder MediaThe SILEX powder is a lyophilizate of the High Performance OD2 Rich Growth
Media. It can be used as a rich growth stand-alone medium (using 10 g powderper L) or as a supplement for M9 fermentations (using 1 g powder per L). Using
SILEX powder as a supplement for M9 increases both cell growth and protein expression rates. See results in our SILEX powder media information leaflet.

ECO Powder MediaSilantes ECO powder media are a more economical alternative to SILEX powder media, adjusted to ensure the same performance as SILEX powder. It contains the same
ingredients as the SILEX powder while having a slightly higher amount of low molecular weight oligopeptides.

www.silantes.com|

www.silantes-shop.com|

0049 (0)89 500 941 0|

info@silantes.com