Toxicity of Lipid Nano Particles (causing liver damage)
By Craig Paardekooper

What are Cationic Lipids ?
Cationic and ionizable cationic lipids are small molecules with a positive charge. They form electrostatic bonds orcomplexes with negatively charged molecules such as nucleic acids and cell membranes. Consequently, they are utilized
to improve cellular membrane permeability – by merging with a cell membrane, they can then release their payload.

The Lipids in the COVID-19 Jab are Ionizable Cationic Lipids“Current prominent examples are the ionizable cationic lipids ALC-0315 [[(4-hydroxybutyl)azanediyl]di(hexane6,1-diyl) bis(2-hexyldecanoate))] and Lipid H (SM-102) (9-heptadecanyl 8-{(2-hydroxyethyl)[6-oxo-6(undecyloxy)hexyl]amino}octanoate) that are used for coronavirus disease 2019 (COVID-19) mRNA vaccines
BNT 162b and mRNA-1273, respectively.[1]”References
https://onlinelibrary.wiley.com/doi/10.1002/smll.202206968
https://pubmed.ncbi.nlm.nih.gov/34811367/:

The Dangers of Cationic Lipids“The use of cationic and ionizable cationic lipids in pharmaceutical products, however, is a double-edged sword,
as these excipients are of considerable safety concerns. Because of their permanent or pH-dependent cationicnature, they perturbate cellular and nuclear membranes, trigger the release of degrading enzymes fromlysosomes, cause mitochondrial permeabilization and dysfunction, generate reactive oxygen species (ROS),
alter cytoplasmatic enzyme functions, and damage DNA.[3]References
https://onlinelibrary.wiley.com/doi/10.1002/smll.202206968:

The above quote says that cationic lipids have considerable safety concerns. This arises from the fact that they haveeither –1. A permanent positive charge or
2. A positive charge that appears under certain pH condition
The effect of this positive charge is that it –
1.
2.
3.
4.
5.

Disturbs cellular and nuclear membranes
Triggers the release of degrading enzymes from lysosomes
Causes dysfunction of mitochondria
Generates reactive oxygen species
Damages DNA

All these are effects of its positive charge.

These cationic lipids have been investigated for over 30 years since cationic lipid-based gene delivery (lipofection) was
first published by Felgner’s group in 1987, but have not found their way into general application because of theirtoxicity.
It is not just cellular biologists with experience of this field who state that cationic lipids are “very, very toxic”. A peerreviewed paper in Toxicology Research from April 2018 states in the opening sentence of its abstract: “cationic lipids
still have the problem of toxicity, which has become one of the main bottlenecks for their applications.”. Thisfinding is reflected throughout the literature on cationic lipids, across the entire three decades. An article as recent asMay 2019, “Lipid Nanoparticles for Delivery of Therapeutic RNA Oligonucleotides” says “A major drawback with theuse of cationic lipids for gene delivery is the high net positive charge associated with the headgroup as well as inductionof immune response ... Furthermore, particles of cationic nature are known to undergo accumulation in the liver, lung,and spleen.”
Reference
603f7c2bc3f872e7e67deb52_Potential_risk_of_cationic_lipid_in_the_Pfizer-BioNTech_vaccine.pdf (webflow.com):

Attempted Solutions?
A. Ionizable
The lipid only becomes positively charged when it has been taken into a cell by the process of endocytosis.
Within the endosome, the lipid is subjected to a low pH environment, and this triggers the lipid to becomecationic. The pH has to be quite low (pH=5) for this to happen. Then the cationic lipid releases its payload.
However, a question arises as to what becomes of the cationic lipid afterwards.
B. Biodegradable alternatives have been introduced that are rapidly degraded in vivo, so that the toxic effects
are “short-lived”. How is this done?
A cationic lipid consists of 3 parts
1. A polar head (the charged part)
2. A fatty hydrophobic tail (the lipid part)
3. A bond linking the above two parts (either an ester or an amide bond)
The bodies enzymes attack the ester or amide bond breaking it – so the cationic lipid breaks up into a charged
head and a lipid tail – it degrades. However, a question arises as to how fast this degradation takes place.

2 Weeks Later
But, as we shall see, the cationic lipids associated with the COVID-19 mRNA jabs do not degrade fast enough. They
have been found in the circulatory system more than 2 weeks after the shot.
After ester cleavage of ALC-0315, the doubly de-esterified metabolite still exhibits a lipophilic ionizablecationic character that is further metabolized by glucuronidation, followed by urinary excretion.[53]Nonetheless, considerable amounts of ALC-0315 were found in the liver two weeks after administration,
whereas SM-102 and its degradation products were more rapidly eliminated via the renal and biliary route.[53,54]
This might be explained by the linear fatty alcoholic tail of SM-102 that contributes to a higher accessibility
for enzymatic cleavage of the first ester bond and subsequently also the second as a result of a reduced sterichindrance.
References
https://onlinelibrary.wiley.com/doi/10.1002/smll.202206968
https://www.ema.europa.eu/en/documents/assessment-report/comirnaty-epar-public-assessmentreport_en.pdf
2 weeks later high concentrations of the lipid ALC-0315 were found in the liver.

:PK studies with the two novel LNP-excipients ALC-0315 and ALC-0159: Wistar Han rats were IV bolus injectedwith LNP formulated luciferase-encoding RNA at 1 mg/kg and ALC-0315 and ALC-0159 concentrations at 15,3mg/kg and 1,96 mg/kg respectively. ALC-0315 and ALC-0159 levels in plasma, liver, urine and faeces wereanalysed by LC-MS/MS at different time-points up to 2-weeks.
ALC-0315 and ALC-0159 were rapidly cleared from plasma during the first 24 hours with an initial t½
of 1.62 and 1.72 h, respectively. 24 hours post-dosing, less than 1% of the maximum plasma
concentrations remained. A slower clearance rate was observed after 24 hours with ALC-0315 andALC-0159 terminal elimination t½ of 139 and 72.7 h, respectively. Following plasma clearance, the liverappears to be to major organ to which ALC-0315 and ALC-0159 distribute. The applicant has estimated thepercent of dose distributed to the liver to be ~60% for ALC-0315 and ~20% for ALC-0159. The observed liver
distribution is consistent with the observations from the biodistribution study and the repeat-dose toxicology,
both using IM administration. For ALC-0315 (aminolipid), the maximum detected concentration in the liver(294 μg/g liver) was reached 3 hours after IV injection. ALC-0315 was eliminated slowly from the liver andafter 2-weeks the concentration of ALC-0315 was still ~25% of the maximum concentration indicating thatALC-0315 would be eliminated from rat liver in approximately 6-weeks. For ALC-0159 (PEG-lipid), themaximum detected concentration in the liver (15.2 μg/g liver) was reached 30 minutes following IV injection.
ALC-0159, was eliminated from the liver faster than ALC-0315 and after 2-weeks the concentration of ALC0159 was only ~0,04% of the maximum detected concentration. The applicant was asked todiscuss the long half-life of ALC-0315 and its effect, discussion on the comparison with patisiran, as
well as the impact on the boosts and post treatment contraception duration. The applicant considered that
there were no non-clinical safety issues based on the repeat dose toxicity studies at doses (on a mg/kg basis)much greater than administered to humans; this was acceptable to the CHMP.”“While there was no detectable excretion of either lipid in the urine, the percent of dose excreted
unchanged in faeces was ~1% for ALC-0315 and ~50% for ALC-0159”
Reference :https://www.ema.europa.eu/en/documents/assessment-report/comirnaty-epar-public-assessmentreport_en.pdf p 45/6
The takeaway from this is that the cationic lipids remain in your body for up to 6 weeks and will cause damage during
this time. In particular, ALC-0315 does not seem to be excreted.
60% of the initial dose becomes concentrated in the liver. Should we expect liver damage?

Liver Damage
If 60% of the LNPs end up in the liver, and if these LNPs readily merge with the liver cells and become cationic, thenwe might expect liver damage.
The primary function of the liver is to filter and purify the blood, so liver damage may result in blood poisoning,
septicaemia, liver disease or liver failure.
Effect on Liver Cells
A detailed analysis of the effect of cationic lipids on liver cells from December 2017 explains the mechanism
as follows - cells were disturbed in amino acid metabolism, energy and lipid supply when cationic lipid
exposure-induced injury occurred.
It is concluded that cationic lipids may induce cytotoxicity by enhancing reactive oxygen species in vitro,
affect the normal process of energy metabolism, disturb several vital signalling pathways and finally induce
cell death.

Reference :Metabolomics revealed the toxicity of cationic liposomes in HepG2 cells using UHPLC-Q-TOF/MS andmultivariate data analysis - PubMed (nih.gov)
Effect on Animals
In an animal trial, submitted as evidence for authorisation of the vaccine by BioNTech to the EMA, thisresulted in high elevations of liver enzymes GGT – “exposure generated increased levels of GGT (>200%) –indicative of liver damage, and AST, which rises in liver and cardiac inflammation.
Given that 60% of the Lipid Nano Particles end up in the liver, merge with liver cells, and become positively
charged, we would expect liver damage to occur, which is what the animal study showed.
Reference :http://radio.rumormillnews.com/pdfs/20201130-BioNTech-Vaccine-Document.pdf

Speeding up Degradation
With ALC-0315, the cationic lipid found in the Pfizer jab, even after it has been doubly de-esterified, it still exhibits apositive charge. A second stage of glucuronidation is required so it is metabolized and excreted in the urine.
SM-102, the cationic lipid found in Moderna jabs, degrades more readily.
It follows that there are 2 ways of speeding up the degradation of the cationic lipids found in the Pfizer jab –1. Increase the rate of breaking of the ester bonds
2. Increase glucuronidation

Here is a link to how to boost glucuronidation - Glucuronidation: Detox, Balance Hormones, & Genes - SelfHackedA Simple Conclusion
Premise 1 : Cationic (positively charged) lipids are highly toxic
Premise 2 : The COVID-19 jab is made of lipids that become cationic when in contact with human cells
Deduction : Therefore, the COVID-19 jab has a degree of toxicity / it cannot be completely safe.

The question then becomes, how dangerous is it ?

CONFIRMED: COVID-19 mRNA Vaccines Can Cause Severe Liver Damage - Dr. Rath Health Foundation (drrath-foundation.org)We might expect that –1. If ionizable cationic lipids are toxic owing to their charge
2. If these lipids induce human cells to produce a foreign protein that causes auto-immune attack
3. If the foreign protein being produced (the spike protein) is intrinsically toxic, and known to cause harmAnd
4. If 60% of these lipids concentrate in the liverthen, liver damage will occur.

A Clear and Present Danger
Given that the World Health Organisation pushed so hard for these shots and promoted them as safe andeffective, is it not dangerous that the same World Health Organisation seeks to amend International HealthRegulations to allow it to mandate medical treatments for all future pandemics upon all 190 member
countries – a medical tyranny?
It seeks not to advise, but to command medical policies for all 190 member states – come the next
“pandemic” – and make these policies binding and mandatory.
It seeks not to allow open discussion, but to suppress any alternative scientific view – labelling such as “misinformation”, and “info-terrorism” – and use constant monitoring and surveillance of subject populations toenforce this.
It should be noted that such regulations would violate all constitutional rights in all countries by imposing
medical treatment without informed consent. Such regulations would also violate the sovereignty of all
member countries by allowing a foreign, unelected body to decide who can travel, who can work, whoshould be put into isolation, and who can have access to resources.
These international Health Regulations will automatically come into effect by default, unless member
governments explicitly opt out within a limited time frame. The default is this tyranny will happen.

What Can You Do?
Should this tyranny become reality –
1.
2.
3.
4.
5.

Don’t comply
Prepare now by developing greater independence of means
Share info and tell many
Get active with local groups
Support MPs who have been outspoken against the lies and have laid their careers on the line for thetruth
6. Support doctors who have been outspoken against the lies and have laid their careers on the line for thetruth
7. Support MPs who can push for direct democracy – and a restitution of your constitutional rights.