シンポジウム

Symposium

1 日目（9 月 28 日（水））／Day 1（Sep. 28 Wed.）
09:00〜11:30 A 会場（函館アリーナ 武道館 A）／Room A（Hakodate Arena Budokan A）
1SAA 動的溶液環境が駆動するタンパク質凝集
Protein aggregation driven by dynamic solution environments
オーガナイザー：吉田 紀生（九州大学），菅瀬 謙治（京都大学）
Organizers: Norio Yoshida (Kyusyu Univ.), Kenji Sugase (Kyoto Univ.)
In cells, the solution environment is constantly changing due to varying concentrations of chemicals, mechanical stimuli,
and electric fields. In recent years, it has become evident that intrinsically disordered proteins, which do not havespecific conformations, undergo liquid-liquid phase separation and amyloid fibrillization in response to the ‘dynamic’solution environment. This series of self-condensation processes is controlled by the protein-protein and protein-solvent
interactions of intrinsically disordered proteins, which have dynamic conformations and solvation states. In thisworkshop, we will discuss the latest research on the self-condensation process of intrinsically disordered proteins andtheir future development.
はじめにOpening Remarks
1SAA-1

TIA-1 プリオン様ドメインの ALS 変異は高度に凝縮した病原性構造体を形成する
ALS mutations in the TIA-1 prion-like domain trigger highly condensed pathogenic structures○関山 直孝 1, 高場 圭章 2, 眞木 さおり 2, 赤木 謙一 3, 大谷 寧子 1, 今村 香代 1, 寺川 剛 1,
山下 恵太郎 4, 米倉 功治 2, 児玉 高志 1, 杤尾 豪人 1（1 京大・理・生物物理, 2 理研・放射光科学研究センター, 3 医薬基盤・健康・栄養研究所, 4MRC 分子生物学研究所）
Naotaka Sekiyama1, Kiyofumi Takaba2, Saori Maki-Yonekura2, Ken-ichi Akagi3, Yasuko Ohtani1,
Kayo Imamura1, Tsuyoshi Terakawa1, Keitaro Yamashita4, Koji Yonekura2, Takashi Kodama1,
Hidehito Tochio1 (1Dept. of Biophysics, Grad Sch. of Science, Kyoto Univ., 2RIKEN SPring-8 Center,
3NIBIO, 4MRC Laboratory of Molecular Biology)

1SAA-2

流れが駆動するタンパク質のアミロイド線維化機構
Mechanism of amyloid fibrillation of a protein driven by a flow
○菅瀬 謙治 1, 森本 大智 2, Erik Walinda3（1 京都大学大学院農学研究科 応用生命科学専攻, 2 京都大学大学院工学研究科分子工学専攻, 3 京都大学大学院医学研究科細胞機能制御学）
Kenji Sugase1, Daichi Morimoto2, Erik Walinda3 (1Division of Applied Life Sciences, Graduate Schoolof Agriculture, Kyoto University, 2Department of Molecular Engineering, Graduate School ofEngineering, Kyoto University, 3Department of Molecular and Cellular Physiology, Graduate School of
Medicine, Kyoto University)

1SAA-3生体分子のための動的溶媒和理論の開発
Development of Dynamic Solvation Theory for Biomolecules○吉田 紀生（名古屋大・院情報）
Norio Yoshida (Grad. Sch. Info., Nagoya Univ.)– S57 –

1SAA-4リン酸化による HP1α の液-液相分離機構の解明
Phase separation mechanism of HP1α by phosphorylation○古川 亜矢子 1, 米澤 健人 2,3, 根上 樹 4, 吉村 ゆり子 5, 林 亜紀 5, 中山 潤一 5,6, 安達 成彦 2,
千田 俊哉 2, 清水 謙多郎 4, 寺田 透 4, 清水 伸隆 2, 西村 善文 1,7（1 横市大・生命医科学, 2 高エネ機
構・物構研, 3 奈良先端大・CDG, 4 東大・院農, 5 基生研, 6 総研大・生命科学研究科, 7 広島大・統合生命）
Ayako Furukawa1, Kento Yonezawa2,3, Tatsuki Negami4, Yuriko Yoshimura5, Aki Hayashi5,
Jun-ichi Nakayama5,6, Naruhiko Adachi2, Toshiya Senda2, Kentaro Shimizu4, Tohru Terada4,
Nobutaka Shimizu2, Yoshifumi Nishimura1,7 (1Grad. Sch. Med. Life Sci., Univ. Yokohama City, 2IMSS,
KEK,, 3NAIST, CDG, 4Grad. Sch. Agr. Life Sci., Univ. Tokyo, 5NIBB, 6SOKENDAI, 7Grad. Sch. Integ. Sci.
Life, Univ. Hiroshima)

1SAA-5タンパク質の変性状態における構造ダイナミクスと溶媒環境依存性の理論的解析
Theoretical study on the conformational dynamics of proteins in disordered states under
different solvent environments○森 俊文（九大・先導研）
Toshifumi Mori (Inst. Mat. Chem. Eng., Kyushu Univ.)

1SAA-6

ポリマーコートされたダイヤモンドナノ粒子によるバイオセンシング
Biosensing Using Diamond Nanoparticles Coated with Polymers○外間 進悟, 原田 慶恵（阪大・蛋白研）
Shingo Sotoma, Yoshie Harada (IPR, Osaka Univ.)

1SAA-7(2Pos051) 蝶々型金ナノデバイスが可能にするタンパク質液液相分離過程の制御(2Pos051) Control of protein condensation by butterfly-shaped gold nanodevices○延山 知弘 1, 高田 耕児 2, 村上 達也 2, 白木 賢太郎 1,2（1 筑波大・応用理工, 2 富山県立大・院工）Tomohiro Nobeyama1, Koji Takata2, Tatsuya Murakami2, Kentaro Shiraki1,2 (1Pure and Appli.Sci.,
Univ.Tsukuba, 2Grad. Sch. Sci. Toyama Pref. Univ)

1SAA-8(1Pos054) GGGGCC-RNA は、TDP43 およびそのカルボキシ断片の凝集を抑制する(1Pos054) GGGGCC-RNA prevents aggregation of TDP43 and its carboxy terminal fragments○藤本 愛 1, 金城 政孝 2, 北村 朗 2（1 北大・院・生命, 2 北大・先端生命）
Ai Fujimoto1, Masataka Kinjo2, Akira Kitamura2 (1Grad. Sch. of Life Sci., Hokkaido. Univ, 2Fac. Adv.
Life Sci., Hokkaido. Univ)
おわりに
Closing Remarks– S58 –

09:00〜11:30 B 会場（函館アリーナ 武道館 B）／Room B（Hakodate Arena Budokan B）
1SBA 量子ビームでひも解くタンパク質の大きな構造変化 –タンパク質ダイナミクス理解の新潮流
Protein large-scale motions revealed by quantum beams -a new era in understanding protein
dynamics
オーガナイザー：山本 直樹（自治医科大学），関口 博史（高輝度光科学研究センター）
Organizers: Naoki Yamamoto (Jichi Med. Univ.), Hiroshi Sekiguchi (JASRI)
Biological systems function by constantly changing their hierarchical and inter-hierarchical interactions amongmolecules, cells, and individuals. In order to visualize these dynamics, it is effective to approach them using penetrating
quantum beams such as X-rays and neutron beams. This symposium will introduce recent advances in quantum beamtechniques for biophysical research, mainly focusing on large structural changes within and between protein molecules.
Furthermore, molecular dynamics simulation studies combined with the experimental researches, which deepen theknowledge on molecular mechanisms of the complexed protein systems, will also be shown.
はじめにOpening Remarks
1SBA-1X 線 1 分子追跡法によるヘモグロビン・アロステリーの再検討
Allosteric Transition Dynamics in Hemoglobin Reconsidered by Diffracted X-ray Tracking○関口 博史 1, 山本 直樹 2, 柴山 修哉 2, 佐々木 裕次 1,3（1 高輝度光科学研究センター, 2 自治医科
大・医, 3 東大・新領域）
Hiroshi Sekiguchi1, Naoki Yamamoto2, Naoya Shibayama2, Yuji Sasaki1,3 (1JASRI/SPring-8, 2Dept.
Physiology, Jichi Med. Univ., 3Grad. Sch. Front. Sci., Univ. Tokyo)

1SBA-2

Internal dynamics of intrinscially disordered protein as studied by neutron scattering
Rintaro Inoue (Institute for Integrated Radiation and Nuclear Science, Kyoto University)

1SBA-3(2Pos050) タンパク質ケージ内における芳香環相互作用ネットワークの熱力学・分子動力学的解析(2Pos050) Thermodynamic and Molecular Dynamic Analysis of Aromatic Interaction Networks
in Protein Cages○菱川 湧輝 1, 野谷 大樹 1, 浅沼 明日香 1, Maity Basudev1, 長門石 曉 2, 津本 浩平 2,3, 安部 聡 1,
上野 隆史 1（1 東工大・生命理工, 2 東大・医科研, 3 東大・院工）Yuki Hishikawa1, Noya Hiroki1, Asuka Asanuma1, Basudev Maity1, Satoru Nagatoishi2,
Kouhei Tsumoto2,3, Satoshi Abe1, Takafumi Ueno1 (1Sch. Life Sci. Technol., Tokyo Inst. Technol., 2Inst.
Med. Sci., Univ. Tokyo, 3Sch. Eng., Univ. Tokyo)

1SBA-4(1Pos076) 3D structural determination of proteins from fluctuation X-ray scattering data
Wenyang Zhao1, Osamu Miyashita1, Florence Tama1,2 (1Center for Computational Science, RIKEN,
2Grad. Sch. Sci., Univ. Nagoya)

1SBA-5Microscopic Mechanisms of Stable Amyloid β (1-42) Oligomer Formation
Ikuo Kurisaki, Shigenori Tanaka (Graduate School of System Informatics, Kobe University)– S59 –

1SBA-6

非干渉性中性子散乱で観測するアミロイド構造多形体及びリン脂質分子のサブナノ秒ダイナミクス
Sub-nanosecond dynamics of amyloid polymorphs and phospholipid molecules observed byincoherent neutron scattering○松尾 龍人 1,2,3, Francesco Alessio De2, Cissé Aline2,3, Bicout Dominique2,3, Peters Judith2,3,4（1 量研・量子生命科学研究所, 2Institut Laue-Langevin, France, 3Université Grenoble Alpes, France, 4Institut
Universitaire de France）
Tatsuhito Matsuo1,2,3, Alessio De Francesco2, Aline Cissé2,3, Dominique Bicout2,3, Judith Peters2,3,4(1iQLS, QST, 2Institut Laue-Langevin, France, 3Université Grenoble Alpes, France, 4Institut
Universitaire de France)

1SBA-7

アミロイド線維前駆中間体の構造発達とその阻害
Structural development of amyloid prefibrillar intermediates and its inhibition○山本 直樹（自治医大・医）Naoki Yamamoto (Sch. Med. Jichi Med. Univ.)
おわりにClosing Remarks

09:00〜11:30 C 会場（函館アリーナ 武道館 C）／Room C（Hakodate Arena Budokan C）
1SCA 【共催：新学術研究領域「生命金属科学」】
生命金属のライブセルイメージング
Live-cell imaging of bio-metal species
オーガナイザー：石森 浩一郎（北海道大学），平山 祐（岐阜薬科大学）
Organizers: Koichiro Ishimori (Hokkaido Univ.), Tasuku Hirayama (Gifu Pharm. Univ.)
The inorganic ions are essential for life despite their small amounts, in addition to organic macromolecules such asproteins, DNA, carbohydrates, and lipids. The dysfunction of the homeostasis of these inorganic ion species is involvedin various pathologies. To understand the dynamics and functions of the inorganic species in living things, theobservation of their existence and fluctuation in living cells is necessary. In this symposium, up-and-coming researchers
will give talks on innovative methods for imaging inorganic species in living cells. This symposium is a collaborative
symposium with Integrated Metal-bioscience.
1SCA-1

遊離鉄およびヘム蛍光プローブのライブセルイメージングへの応用
Fluorescence probes for labile iron and heme and their applications to live-cell imaging○平山 祐（岐阜薬科大学薬化学研究室）
Tasuku Hirayama (Lab. Pharm. Med. Chem., Gifu Pharm. Univ.)

1SCA-2

細胞内遊離亜鉛イオンの定量解析を可能とする小分子蛍光プローブの開発
Development of small-molecule fluorescent probes for the quantitative analysis of labile Zn2+ incells○小和田 俊行（東北大学多元物質科学研究所）
Toshiyuki Kowada (Institute of Multidisciplinary Research for Advanced Materials, Tohoku University)

1SCA-3

コンディショナルプロテオミクスによる金属関連タンパク質のイメージングとプロファイリング
Imaging and profiling of metal-related proteins by conditional proteomics strategies○田村 朋則（京大院工）Tomonori Tamura (Grad. Sch. Eng., Univ. Kyoto)– S60 –

1SCA-4

量子ビームによる生命金属シングルセルイメージング
Single cell imaging by quantum beam elemental analyses for dynamics of cellular distributionof bio-metals○武田 志乃（国立研究開発法人量子科学技術研究開発機構 放射線医学研究所）
Shino Takeda (National institute of Radiological Sciences, National Institutes for Quantum Science andTechnology)

1SCA-5蛍光イメージングで解き明かす細胞内マグネシウムイオンの役割
The roles of intracellular magnesium ion revealed by fluorescence imaging○新藤 豊（慶大・理工・生命情報）
Yutaka Shindo (Dept. Biosci. Info., Keio Univ.)

1SCA-6

H2S 検出蛍光プローブの開発とそれを用いた活性イオウ分子産生酵素の阻害剤スクリーニングへの応用
Development of a fluorescence probe for H2S and its application to the inhibitor screening ofreactive sulfur species-producing enzymes○花岡 健二郎（慶應大・院薬）
Kenjiro Hanaoka (Grad. Sch. Pharm. Sci., Keio Univ.)

09:00〜11:30 D 会場（函館アリーナ 多目的室 A）／Room D（Hakodate Arena Multipurpose Room A）
1SDA 糖鎖の動的構造から機能へ – 実験・理論解析の最先端
Unveil glycansʼ function from their dynamical structures. – Cutting-edge challenges
オーガナイザー：李 秀栄（医薬基盤・健康・栄養研究所），山口 芳樹（東北医科薬科大学）
Organizers: Suyong Re (NIBIOHN), Yoshiki Yamaguchi (Tohoku Med. and Pharm.l Univ.)
Glycosylation of proteins is a ubiquitous biomolecular process. It adds extra functions or modulates existing functions ofproteins, thereby affecting a range of cellular processes and diseases. Despite of the complex and dynamical nature ofglycan structures, the recent advances both in experiment and computation enable us to investigate their functions basedon the dynamical structures at atomic resolution, as exemplified in the “glycan-shield” of SARS-CoV-2 spike protein. In
this symposium, we would like to share the cutting-edge challenges in determining functional structures and dynamics
of glycans and discuss the potential future collaborations.
はじめに
Opening Remarks
1SDA-1

Viral glycosylation: HIV-1 to SARS-CoV-2
Max Crispin (School of Biological Sciences, University of Southampton, UK)

1SDA-2Integrative methods in structural glycobiology
Jon Agirre (University of York)

1SDA-3

GLYCO: a tool to quantify glycan shielding of glycosylated proteins
Myungjin Lee (National Institutes of Health)

1SDA-4

Psme3 の部位特異的な O-GlcNAc 修飾は、P-body の恒常性の阻害を介してマウス ES 細胞の多能性維持に関与する
Site-specific O-GlcNAcylation of Psme3 maintains mouse embryonic stem cell pluripotency by
impairing P-body homeostasis○西原 祥子（創価大学・糖鎖生命システム融合研究所）
Shoko Nishihara (Glycan & Life System Integration Center (GaLSIC), Soka University)– S61 –

1SDA-5

Description of the dynamic conformation of oligosaccharides by combining NMR spectroscopy
and molecular simulation
Takumi Yamaguchi1,2 (1School of Materials Science, Japan Advanced Institute of Science andTechnology, 2Graduate School of Pharmaceutical Sciences, Nagoya City University)

1SDA-6

病原性細菌における付着因子の糖鎖認識機構
Analyses of recognition mechanism and structure of bacteria FimH adhesin○能登 香（北里大学・一般教育）
Kaori Ueno-Noto (Coll. Lib. Arts Sci., Kitasato Univ.)

09:00〜11:30 E 会場（函館アリーナ 多目的室 B）／Room E（Hakodate Arena Multipurpose Room B）
1SEA 【共催：CREST 「新たな光機能や光物性の発現・利活用を基軸とする次世代フォトニクスの基盤技術」】
先端赤外光の利用による生物物理学研究
Utilization of Advanced Infrared Sources for Biophysical Studies
オーガナイザー：古谷 祐詞（名古屋工業大学），村越 秀治（生理学研究所）
Organizers: Yuji Furutani (NITech), Hideji Murakoshi (NIPS)
Infrared (IR) light has been widely utilized for analyzing molecular structure and interaction in biological and organic
materials. Nowadays, new infrared light sources have been developed, such as ultrafast pulsed IR lasers, quantum
cascade lasers, and fiber lasers. These lasers are applicable not only to vibrational spectroscopy on biological molecules
but also to microscopic imaging of biological systems such as tissues and cells. Multi-photon microscopy is one of themost important applications, which can shed light on deep inside brains. In this symposium, we would like to discuss thepossibility of new infrared light sources in biophysical studies.
はじめに
Opening Remarks
1SEA-11800 nm フェムト秒ファイバーレーザーを用いた多光子蛍光顕微鏡
Multi-photon fluorescence microscopy using a 1800 nm femtosecond fiber laser system○藤 貴夫 1, 村越 秀治 2,3, 植田 大海 2,3, 濱田 航輔 1, 後藤 龍一郎 4（1 豊田工業大学, 2 生理学研究所, 3 総合研究大学院大学, 4 ファイバーラボ株式会社）
Takao Fuji1, Hideji Murakoshi2,3, Hiromi Ueda2,3, Kosuke Hamada1, Ryuichiro Goto4 (1Toyota
Technological Institute, 2National Institute for Physiological Sciences, 3The Graduate University for
Advanced Studies, 4Fiberlabs Inc.)

1SEA-2

高機能超短パルスファイバレーザーを用いた第 3 の生体の窓における生体深部イメージング
Deep tissue imaging using highly functional ultrashort pulse fiber laser in the third NIR opticaltissue window○西澤 典彦 1, 山中 真仁 2（1 名古屋大学工学研究科電子工学専攻, 2 大阪大学工学研究科応用物理学専攻）
Norihiko Nishizawa1, Masahito Yamanaka2 (1Department of Electronics, Nagoya University,
2Department of Applied Physics, Osaka University)

1SEA-3非線形光学過程を利用した 2 種類の赤外超解像顕微鏡による生体試料の観察
Selective IR super-resolution imaging of biological samples by micro-spectroscopies based on
non-linear optical process○高橋 広奈, 酒井 誠（岡山理大・理）Hirona Takahashi, Makoto Sakai (Faculty of Sci., Okayama Univ. of Sci.)– S62 –

1SEA-4

ロドプシンをモデルとした膜タンパク質の表面増強赤外分光計測による構造変化解析
Structural changes of rhodopsin studied by surface-enhanced infrared spectroscopy as a model
system of membrane proteins○古谷 祐詞 1,2（1 名工大・院工, 2 名工大・オプトバイオ）
Yuji Furutani1,2 (1Grad. Sch. Eng., Nagoya Inst. Tech., 2OptoBio, Nagoya Inst. Tech.)

1SEA-5

高速中赤外分光および顕微鏡
High-speed mid-infrared spectroscopy and microscopy○井手口 拓郎（東京大学・フォトンサイエンス研究機構）
Takuro Ideguchi (IPST, Univ. Tokyo)
おわりにClosing Remarks

09:00〜11:30 G 会場（函館市民会館 3F 小ホール）／Room G（Hakodate Citizen Hall 3F Small Hall）
1SGA 再構築実験によってアプローチするプロトセル研究と生命の起源への探求
Frontiers of Protocell Research: Exploring the Origin of Life through a Constructivist Approach
オーガナイザー：車 兪澈（海洋研究開発機構），Tony Z. Jia（東京工業大学）
Organizers: Yutetsu Kuruma (JAMSTEC), Tony Z. Jia (Tokyo Tech)
The search for the origin of life (OoL) is now entering a new phase, involving researchers from various fields andincorporating new scientific findings. Especially, the study of artificial cells by a constructivist approach and assemblyof protocells by liquid-liquid phase separation or other non-“traditional” physical processes have been advocating a newinterpretation in OoL studies. This symposium will focus on the frontiers of the construction and assembly of protocells
and artificial cells with novel emergent structures and functions relevant to the origins of life.
はじめに
Opening Remarks
1SGA-1

DNA ナノテクノロジーを軸に挑む人工分子システム構築
Toward the construction of cell-like molecular systems based on DNA nanotechnology○佐藤 佑介（九工大・院情報工）
Yusuke Sato (Fac. Comp. Sci. and Sys. Eng., Kyushu Inst. Tech.)

1SGA-2(2Pos120) 人工核酸 PNA を用いた DNA の液－液相分離制御(2Pos120) Regulation of liquid-liquid phase separation of DNA using peptide nucleic acid (PNA)○相馬 陸杜, 愛場 雄一郎, 柴田 将成, 有安 真也, 荘司 長三（名古屋大学大学院理学研究科）
Rikuto Soma, Yuichiro Aiba, Masanari Shibata, Shinya Ariyasu, Osami Shoji (Graduate School of
Science, Nagoya University.)

1SGA-3

Investigating the role of membrane biophysical properties on protein folding and sorting
Neha Kamat1,2 (1Department of Biomedical Engineering, Northwestern University, 2Center of Synthetic
Biology, Northwestern University)

1SGA-4脂質を合成する人工細胞
Lipid synthesis in artificial cell
Yutetsu Kuruma1,2, Yasuhiro Shimane1, Rumie Matsumura1 (1JAMSTEC, 2JST PRESTO)– S63 –

1SGA-5RNA 複製と進化のための区画構造
Compartmentalization for RNA replication and evolution○水内 良 1,2（1 東大・先進科学, 2JST・さきがけ）
Ryo Mizuuchi1,2 (1Komaba Inst. Sci., Univ. Tokyo, 2JST, PRESTO)

1SGA-6

エクソソームが司る、がん転移の新しいストーリー
Exosomes, new players in the field of metastasis○星野 歩子（東京工業大学）
Ayuko Hoshino (Tokyo Institute of Technology)
おわりにClosing Remarks

09:00〜11:30 H 会場（函館市民会館 3F 大会議室）／Room H（Hakodate Citizen Hall 3F Conference
Room）
1SHA 分子集団，細胞集団が織りなす自律特性: 生命機能の理解を目指して
Autonomous Characteristics of Molecular and Cellular Ensembles: Toward an Understanding ofBiological Functions
オーガナイザー：茅 元司（東京大学），島本 勇太（国立遺伝学研究所）
Organizers: Motoshi Kaya (The Univ. of Tokyo), Yuta Shimamoto (NIG)
The autonomous characteristics of molecular and cellular assemblies are of a higher order than can be imagined from thecharacteristic of a single molecule or cell, and are the essence of various biological functions. In this symposium, we
will invite researchers who are working on the mechanisms of cell motility, tissue formation, etc., using a variety ofadvanced approaches. We will discuss how understanding the autonomous characteristics of molecular and cellularensembles can advance our understanding of biological functions.
1SHA-1

骨格筋ミオシン，心筋ミオシン分子集団の自律特性が骨格筋，心臓収縮を作り出す
Autonomous characteristics of skeletal and cardiac myosin ensembles are essential forcontractile functions of skeletal muscle and heart○茅 元司（東大・院理）Motoshi Kaya (Grad. Sch. Sci., Univ. Tokyo)

1SHA-2

人工細胞による細胞動態の再構築：自発運動から波動現象の力学的理解へ向けて
Artificial cells: Reconstruction of cell-like behaviors from spontaneous migration to wave
dynamics toward understanding cell mechanics
Ryota Sakamoto1,2, Ziane Izri3, Yuta Shimamoto4, Makito Miyazaki5,6,7,8, Yusuke Maeda2 (1Dept.
Biomed. Engr., Yale Univ., 2Dept. Phys., Kyushu Univ., 3Dept. Phys., Minessota Univ., 4Natl. Inst.
Genetics, 5Hakubi Ctr., Kyoto Univ., 6Dept. Phys., Kyoto Univ., 7Institut Curie, 8JST PRESTO)

1SHA-3

紡錘体の自己組織化ダイナミクスと微小管の集団運動メカニクス
Morphological growth dynamics and active microtubule mechanics underlying spindle selforganization○島本 勇太（国立遺伝学研究所）
Yuta Shimamoto (National Institute of Genetics)

1SHA-4

時計回りの組織形成を支える集団細胞移動の作動原理
Mechanical perspective of collective cell movement in epithelial morphogenesis○倉永 英里奈（東北大・院生命科学）
Erina Kuranaga (Grad. Sch. Life Sci., Tohoku Univ.)– S64 –

1SHA-5魚類表皮ケラトサイト集団ではリーダー細胞とフォロワー細胞が協調的かつ強制的にフォロワー
をリーダーに昇進させる
Cooperative but forcible promotion of follower cells to leaders in collective migration of fish
keratocytes○岩楯 好昭（山口大・理）Yoshiaki Iwadate (Dept. Biol., Yamaguchi Univ.)

1SHA-6

Interaction rules within multicellular dynamics and biological condensates
Kawaguchi Kyogo (RIKEN CPR/BDR)

13:50〜16:20 A 会場（函館アリーナ 武道館 A）／Room A（Hakodate Arena Budokan A）
1SAP 【共催：文部科学省「富岳」成果創出加速プログラム「プレシジョンメディスンを加速する創薬ビッグデータ統合システムの推進」】
スーパーコンピューター「富岳」による創薬・医療の革新
Innovation of drug discovery and medical treatment using supercomputer Fugaku
オーガナイザー：荒木 望嗣（京都大学），池口 満徳（横浜市立大学）
Organizers: Mitsugu Araki (Kyoto Univ.), Mitsunori Ikeguchi (Yokohama City Univ.)
Drug discovery and medical technologies are being innovated by development of high performance computing (HPC).
Large-scale molecular dynamics simulations performed on supercomputer Fugaku permit atomic-level observation of“slow” biomolecular processes such as protein conformational transition and protein-drug interaction processes,
providing deeper insight into molecular mechanisms of disease and drug design to overcome it. In this symposium, we
will discuss about the forefront of next-generation molecular simulation techniques for drug discovery and medical
treatment.
はじめにOpening Remarks
1SAP-1超高周波超音波照射下での分子動力学シミュレーションによるタンパク質-医薬品結合プロセスの加速
Hypersound-perturbed molecular dynamics to accelerate slow protein-ligand binding processes○荒木 望嗣, 奥野 恭史（京大・院医）
Mitsugu Araki, Yasushi Okuno (Grad.Sch.Med., Kyoto Univ.)

1SAP-2

スーパーコンピューター「富岳」による創薬へ向けた自由エネルギー摂動法の開発
Development of the free-energy perturbation method toward drug discovery on supercomputer
Fugaku○尾嶋 拓 1, 杉田 有治 1,2,3（1 理研・BDR, 2 理研・R-CCS, 3 理研・CPR）
Hiraku Oshima1, Yuji Sugita1,2,3 (1RIKEN BDR, 2RIKEN R-CCS, 3RIKEN CPR)

1SAP-3

Binding Kinetics of Kinase Complexes by PaCS-MD/MSM
Kazuhiro Takemura, Akio Kitao (SLST, TokyoTech)

1SAP-4

MD シミュレーションで考える抗原-抗体界面：合理的な抗体医薬品設計に向けて
A molecular dynamics study on the antigen-antibody interface: Toward rational antibody drug
design○山下 雄史（東京大学）
Takefumi Yamashita (The University of Tokyo)– S65 –

1SAP-5大規模分子動力学シミュレーションを用いた上皮成長因子受容体キナーゼの活性化メカニズムの研究
A study of activation mechanism of epidermal growth factor receptor kinase using large-scale
molecular dynamics simulations○井上 雅郎 1, 浴本 亨 1, 山根 努 2, 池口 満徳 1,2（1 横浜市大・院生命医科学, 2 理研・計算科学研究センター）
Masao Inoue1, Toru Ekimoto1, Tsutomu Yamane2, Mitsunori Ikeguchi1,2 (1Grad. Sch. Med. Life Sci.,
Yokohama City Univ., 2RIKEN R-CCS)

1SAP-6Extracting protein dynamics from experimental cryo-EM maps using a machine learningtechnique combining with MD simulations
Shigeyuki Matsumoto1, Shoichi Ishida2, Kei Terayama2, Yasushi Okuno1,3 (1Grad. Sch. Med., Kyoto
Univ., 2Grad. Sch. Med. Life Sci., Yokohama City Univ., 3RIKEN R-CCS)
おわりに
Closing Remarks

13:50〜16:20 B 会場（函館アリーナ 武道館 B）／Room B（Hakodate Arena Budokan B）
1SBP 【共催：学術変革領域研究（A）「クロススケール新生物学」】
細胞内メゾ複雑体の構造と機能
Structure and function of “meso-entangled bodies” in the cell
オーガナイザー：杉田 有治（理化学研究所），山本 林（東京大学）
Organizers: Yuji Sugita (RIKEN), Hayashi Yamamoto (The Univ. of Tokyo)
Proteins often assemble to form "mesoscopic" complexes – some ordered and some disordered – to exert their functionsin the cell. Therefore, elucidating their architectures and physical properties is necessary to understand the molecularmechanisms underlying life phenomena and diseases. In this research area "Cross-Scale Biology", we particularly focus
on mesoscopic structures in the range of 20–500 nm (including LLPS condensates), which we define as "mesoentangled bodies (MEBs)", because MEBs are thought to be key factors that determine the fate of organisms through thetransition from disordered to ordered states at the mesoscale. In this symposium, researchers working on the MEBs will
gather to discuss the latest technologies and findings, including In-cell AFM, cryo-EM, chemical biology, and LLPS.
1SBP-1ナトリウムポンプのつくりかた
How to make a sodium pump
○阿部 一啓（名古屋大・細胞生理）
Kazuhiro Abe (CeSPI, Nagoya Univ)

1SBP-2光可逆的蛋白質ラベル化システムによる細胞内蛋白質動態と細胞機能の光制御
Optical control of intracellular protein dynamics and cellular functions using a photoreversible
protein labeling system○水上 進（東北大・多元研）
Shin Mizukami (IMRAM, Tohoku Univ.)

1SBP-3分離した RNP ミセルとしてのパラスペックル核内構造体の構築機構
Construction mechanism of nuclear paraspeckle as an isolated RNP micell○廣瀬 哲郎 1,2, 高桑 央 1,3, 山本 哲也 4, 山崎 智弘 1（1 阪大・院生命機能, 2 阪大・先導学際機構,
3 北大・院医, 4 北大・化学反応拠点）
Tetsuro Hirose1,2, Takakuwa Hiro1,3, Yamamoto Tetsuya4, Yamazaki Tomohiro1 (1Grad. Sch. Front.
Biosci., Osaka Univ, 2OTRI, Osaka Univ, 3Grad. Sch. Med., Hokkaido Univ, 4ICReDD, Hokkaido Univ)– S66 –

1SBP-4

細胞内における酵母プリオン伝播のクロススケール解析
Cross-scale analyais of yeast prion propagation in cells
Motomasa Tanaka (RIKEN Center for Brain Science)

1SBP-5

原子間力顕微鏡を用いた生きた細胞のメゾスケール表面構造体及び内部構造体観察方法の開発
Development of the method for observing mesoscale structures outside and inside living cells
using atomic force microscopy○市川 壮彦 1, Penedo Marcos2, 宮澤 佳甫 1,3, 古庄 公寿 1, Alam Mohammad Shahidul1, 宮田 一輝 1,3,
中村 史 4, 福間 剛士 1,3（1 金沢大・ナノ研, 2EPFL・生物工, 3 金沢大・フロンティア工, 4AIST・細胞工）
Takehiko Ichikawa1, Marcos Penedo2, Keisuke Miyazawa1,3, Hirotoshi Furusho1,
Mohammad Shahidul Alam1, Kazuki Miyata1,3, Chikashi Nakamura4, Takeshi Fukuma1,3 (1NanoLSI,
Kanazawa Univ., 2Inst. Bioeng., EPFL, 3Frontier Eng., Kanazawa Univ., 4CMB, AIST)

1SBP-6Structure modeling of protein complex from experimental data using molecular dynamics
simulation
Takaharu Mori (RIKEN CPR)

1SBP-7Ferritin phase separation driven by NCOA4 promotes two types of ferritin autophagy, macroautophagy and endosomal micro-autophagy
Hayashi Yamamoto (Grad. Sch. Med., Univ. Tokyo)

13:50〜16:20 C 会場（函館アリーナ 武道館 C）／Room C（Hakodate Arena Budokan C）
1SCP 複雑システムの振る舞いの解明に向けたトポロジカルアプローチ
Topological approaches to understand behaviours of complex biological systems
オーガナイザー：望月 敦史（京都大学），岡田 崇（理化学研究所）
Organizers: Atsushi Mochizuki (Kyoto Univ.), Takashi Okada (RIKEN)
It is considered that biological functions emerge from dynamics of complex systems consisting from interactions ofmany biomolecules. Obtaining logical understandings for behaviors of network systems is strongly required in life
sciences. To meet it, a series of mathematical methods have been developed, by which important aspects of dynamical
behaviors are determined from the topology of networks alone. They have been applied to real biological systems andhave made unique achievements. Recently, we see a series of technical or theoretical progresses, which broaden thescope of applications of the methods. In this symposium, we will introduce topological approaches to the network
system, and discuss future perspectives of them.
はじめにOpening Remarks
1SCP-1

ネットワーク構造に基づく細胞運命の制御
Controlling cell fate specification system based on network structure○望月 敦史（京都大・医生研）
Atsushi Mochizuki (Inst. Life Med. Sci, Kyoto Univ.)– S67 –

1SCP-2

摂動後の発現時系列データを用いた遺伝子制御ネットワーク推定法
Estimating gene regulatory network using time-series expression data following gene
perturbation○石川 雅人 1, 永樂 元次 2, 遊佐 宏介 2, 山内 悠平 2, 木立 尚孝 1, 望月 敦史 2（1 東京大・院新領域,
2 京都大・医生研）
Masato Ishikawa1, Mototsugu Eiraku2, Kosuke Yusa2, Yuhei Yamauchi2, Hisanori Kiryu1,
Atsushi Mochizuki2 (1Grad. Sch. Front. Sci., UTokyo, 2Inst. Life Med. Sci., Kyoto Univ.)

1SCP-3

ネットワーク構造から生化学反応の摂動応答を決める
Network architecture determines sensitivity of biochemical reaction systems
Takashi Okada1, Je-Chiang Tsai2, Atsushi Mochizuki1 (1Inst. Life Med. Sci, Kyoto Univ., 2National Tsing
Hua University, Taiwan)

1SCP-4

複雑な化学反応ネットワークを単純化する
Simplifying complex chemical reaction networks
Yuji Hirono (Asia Pacific Center for Theoretical Physics)

1SCP-5

開放系トポロジカル相
Topological phases in open systems○佐藤 昌利（京都大学基礎物理学研究所）
Masatoshi Sato (Yukawa Institute for Theoretical Physics, Kyoto University)

13:50〜16:20 D 会場（函館アリーナ 多目的室 A）／Room D（Hakodate Arena Multipurpose Room A）
1SDP 生体高分子による液液相分離: 基礎と応用
Phase Separation by Biopolymers: Basics and Applications
オーガナイザー：北原 亮（立命館大学），亀田 倫史（産業技術総合研究所）
Organizers: Ryo Kitahara (Ritsumeikan Univ.), Tomoshi Kameda (AIST)
Although cells organize many biochemical processes in membrane-less compartments via liquid-liquid phase separation(LLPS), physicochemical properties and molecular details of LLPS consisting of proteins and nucleic acids are stilllargely unknown. This symposium contains lectures on the physicochemical basis of biomolecular LLPS and somerecent experimental and theoretical developments to elucidate its structure and dynamics. For example, pressure
perturbation spectroscopy, single-molecule fluorescence microscopy, Raman microscopy, and molecular dynamics
simulations for protein LLPS will be introduced.
はじめにOpening Remarks
1SDP-1

圧力ジャンプ法による液液相分離の速度論解析：RNA 結合タンパク質 FUS
Pressure-jump kinetics of liquid-liquid phase separation (LLPS) : The RNA-binding protein fusedin sarcoma (FUS)○北原 亮 1,2, 李 書潔 2, 吉澤 拓也 3（1 立命館大・薬, 2 立命館大院・薬, 3 立命館大・生命）
Ryo Kitahara1,2, Shujie Li2, Takuya Yoshizawa3 (1Coll. Pharm. Sci., Ritsumeikan Univ., 2Grad. Sch.
Pharm. Sci., Ritsumeikan Univ., 3Coll. Life Sci., Ritsumeikan Univ.)

1SDP-2アミノ酸の溶解性に基づくタンパク質の液- 液相分離
Liquid-liquid phase separation of proteins based on the solubility of amino acids○野本 晃, 白木 賢太郎（筑波大院・数理）
Akira Nomoto, Kentaro Shiraki (Pure and Appl. Sci., Univ. Tsukuba)– S68 –

1SDP-3

Raman and Brillouin microscopy as a tool for quantitative study of LLPS
Shinji Kajimoto1,2 (1Grad. Sch. Pharm. Sci., Tohoku University, 2JST PRESTO)

1SDP-4

液–液相分離会合体の分子取り込みと並進拡散運動に関する分子文法解析
Molecular grammar characterization of recruitment and translational dynamics of guest proteinsin liquid droplets○鎌形 清人（東北大多元研）
Kiyoto Kamagata (IMRAM, Tohoku Univ.)

1SDP-5

分子動力学シミュレーションと機械学習を組み合わせたペプチド凝集予測
Prediction of peptide aggregation by combining molecular dynamics simulation and machinelearning○亀田 倫史（産総研・人工知能）
Tomoshi Kameda (AIRC, AIST)
おわりに
Closing Remarks

13:50〜16:20 E 会場（函館アリーナ 多目的室 B）／Room E（Hakodate Arena Multipurpose Room B）
1SEP 微細制御技術を用いたフィジコケミカルバイオロジーへの展開
Physico- and chemical biology using nanomanipulation and micromanipulation technologies
オーガナイザー：北村 朗（北海道大学），飯塚 怜（東京大学）
Organizers: Akira Kitamura (Hokkaido Univ.), Ryo Iizuka (The Univ. of Tokyo)
Various nano- and micromanipulation technologies provide novel strategies to elucidate nature in many scientific fields
such as biophysics, physicobiology, and chemical biology. Here, we introduce the cutting-edge topics using
nanomanipulation and micromanipulation technologies with a single molecule sensitivity, chemical biology,
optogenetics, and mechanistic measurements for understanding and controlling cells and organisms. Furthermore,
research topics in molecular and cellular biology from physicochemical perspectives will be discussed.
はじめにOpening Remarks
1SEP-1

マイクロ・ナノ加工技術を用いた 3D 腫瘍組織構築と新しいがん創薬開発にむけてConstruction of 3D tumor tissue using micro/nano processing technology and toward todevelopment of new drug discovery○繁富（栗林） 香織（北大・高等推進）
Kaori Kuribayashi-Shigetomi (Inst. Adv. High. Edu., Hokkaido Univ.)

1SEP-2

Crosstalk between myosin II and formin in the regulation of force generation and actomyosin
dynamics in stress fibers
Yukako Nishimura1,2, Shidong Shi2, Virgile Viasnoff2,3, Alexander Bershadsky2,4 (1IGM, Hokkaido
Univ., 2MBI, NUS, Singapore, 3Dept.of Biol.Sci., NUS, 4Dept. of Mol. Cell Biol., Weizmann Inst.)

1SEP-3(3Pos312) Triple-color photothermal dye-based nanoheaters to generate multiple heat spots
within a single cell
Md Monir Hossain, Takeru Yamazaki, Kayoko Nomura, Satoshi Arai (Grad. Sch. NanoLS., Kanazawa
Univ.)– S69 –

1SEP-4

Evaluation of the physicochemical properties of biomolecules using microdroplets
Ryo Iizuka (Dept. of Biol. Sci., Grad. Sch. of Sci., The Univ. of Tokyo)

1SEP-5(3Pos093) Control of small G-protein Ras using calmodulin-based ionochromic moleculardevice.
Yassine Sabek, Nobuyuki Nishibe, Kazunori Kondo, Shinsaku Maruta (Graduate school of science andengineering , department of biosciences , soka university, Hachioji TOKYO)

1SEP-6(1Pos283) Centromere-kinetochore structures revealed by 12x modified expansion microscopy
Yasuhiro Hirano1, Aussie Suzuki2, Yasushi Hiraoka1, Tatsuo Fukagawa1 (1Graduate School of Frontier
Biosciences, Osaka University, 2McArdle Laboratory for Cancer Research, University of WisconsinMadison)

1SEP-7

ボトムアップポリマーナノテクノロジーを用いたミクロレベル・マクロレベルの液液相分離制御
Control of the microscopic and macroscopic liquid-liquid phase separation based on bottom-up
polymer nanotechnology○岸村 顕広（九州大学大学院工学研究院応用化学部門）
Akihiro Kishimura (Department of Applied Chemistry, Faculty of Engineering, Kyushu University)

1SEP-8

発色団補助光不活化法 (CALI) の基礎と利用
Basics and applications of chromophore-assisted light inactivation (CALI)○北村 朗（北大・先端生命）
Akira Kitamura (Fac. Adv. Life Sci., Hokkaido Univ.)
おわりにClosing Remarks

13:50〜16:20 F 会場（函館市民会館 1F 大ホール）／Room F（Hakodate Citizen Hall 1F Main Hall）
1SFP 生体膜の生物物理呼応と細胞機能制御への化学的利用
Biophysical responses and biochemical/chemical controlling of membranes for cellular regulationand future therapy
オーガナイザー：中瀬 生彦（大阪公立大学），広瀬 久昭（京都大学）
Organizers: Ikuhiko Nakase (Osaka Metropolitan Univ.), Hisaaki Hirose (Kyoto Univ.)
Biological membranes participate in responses for acceptance/rejection of stimulation and environmental changes fromoutside/inside cells, leading to signal transduction and cellular responses including e.g., cellular uptake, migration,
proliferation, and cell death. The biophysical responses/mechanisms-based membrane controlling systems are highly
anticipated to be next-generation therapeutic methodologies for further achievements of disease regulation such as
cancers. In this proposal symposium, advanced research technologies and achievements of visualizing and controlling
membrane traffic, structures, penetration, and shape-dependent cellular signaling from the fusion viewpoints ofbiophysics, molecular cell biology, chemistry, and chemical biology will be presented, and membrane-based therapeutic
methodology will be discussed.
はじめにOpening Remarks
1SFP-1

Uptake mechanisms of cell-penetrating peptides
Christian Widmann (University of Lausanne, Switzerland)– S70 –

1SFP-2

Roles of membrane lipids in the organization of cell-cell adhestion structure
Junichi Ikenouchi (Grad. Sch. Sci., Kyushu Univ.)

1SFP-3(2Pos198) Mechanism study of antimicrobial peptide synergistic effects at the molecular levelby combining spectroscopy and electrochemical methods
Yuge Hou, Kaori Sugihara (Institute of Industrial Science, The University of Tokyo,)

1SFP-4

Biofunctional peptide-modified exosomes for intracellular delivery
Ikuhiko Nakase (Grad. Sch. Sci., Osaka Metropolitan Univ.)

1SFP-5

Membrane shaping by the BAR domain superfamily proteins and the extracellular vesicles by
the shedding of filopodia
Shiro Suetsugu1,2,3 (1Biological Science, Nara Institue of Science and Technology, 2Data Science Center,
Nara Institue of Science and Technology, 3Digital Green-Innovation, Nara Institue of Science andTechnology)

1SFP-6

Investigating the mechano-osmotic regulation of cell membrane tension using fluorescentmembrane tension probes
Aurelien Roux1,2 (1Department of Biochemistry, CH-1211, University of Geneva, 2NCCR Chemical
Biology, CH-1211, University of Geneva)
おわりに
Closing Remarks

13:50〜16:20 G 会場（函館市民会館 3F 小ホール）／Room G（Hakodate Citizen Hall 3F Small Hall）
1SGP 【共催：JST さきがけ「細胞の動的高次構造体」】
高次構造体を自在に操る
Uncovering the design principles of supramolecular assemblies through manipulation of thestructures, dynamics, and functions
オーガナイザー：宮﨑 牧人（京都大学），小杉 貴洋（分子科学研究所）
Organizers: Makito Miyazaki (Kyoto Univ.), Takahiro Kosugi (IMS)
Cells contains various types of supramolecular assemblies ranging from nanometer-scale structures such as protein
complexes and RNA-protein complexes to micrometer-scale structures such as organelles and liquid droplets. A
growing body of evidence suggests that these ordered and dynamic structures regulate various key functions of the cellwhich were previously unknown or unnoticed. To uncover the design principles of the supramolecular assemblies, notonly identification of the molecular components and observation of the dynamics, but also manipulation of theirstructures, dynamics, and functions will be of crucial importance. In this symposium, we will invite talented early-career
researchers in various research fields who are developing cutting-edge technologies to manipulate the supramolecularassemblies.
はじめに
Opening Remarks
1SGP-1高次構造体の協奏的機能を合理的に制御することを目指して
Toward rational control of concerted functions by supramolecular assemblies○小杉 貴洋 1,2,3,4（1 分子研, 2 総研大, 3 生命創成探究センター, 4JST・さきがけ）
Takahiro Kosugi1,2,3,4 (1IMS, 2SOKENDAI, 3ExCELLS, 4PRESTO, JST)– S71 –

1SGP-2タンパク質合成を司る高次構造体を操る：リボソームの光制御
Controlling a supra-assembly dedicated to protein synthesis: optogenetic control of ribosomesin the cell
○横山 武司 1,2,3（1 東北大・生命科学, 2 東北大・INGEM, 3JST さきがけ）
Takeshi Yokoyama1,2,3 (1Grad. Sch. Lif. Sci., Tohoku Univ., 2INGEM, Tohoku Univ., 3JST PRESTO)

1SGP-3

両親媒性 α ヘリックスが操るオートファジー関連分子 ATG3 の機能
Amphipathic α-helix Manipulates ATG3 Function○西村 多喜 1,2,3, Lazzeri Gianmarco4, 水島 昇 2, Covino Roberto4, Tooze Sharon3（1JST さきがけ専任
研究員, 2 東大・医・分子生物, 3 フランシス・クリック研究所, 4 フランクフルト高等研究所）
Taki Nishimura1,2,3, Gianmarco Lazzeri4, Noboru Mizushima2, Roberto Covino4, Sharon Tooze3 (1JST
PRESTO Researcher, 2Dept. of Biochem & Mol. Biol., Faculty of Med., The Univ. of Tokyo, 3The Francis
Crick Institute, 4Frankfurt Institute for Advanced Studies)

1SGP-4ヘテロクロマチン形成高次構造体の解明と制御
Understanding and reconstructing small RNA mediated heterochromatin formation○岩崎 由香（慶大・医）Yuka Iwasaki (Keio Univ. Sch. Med.)

1SGP-5操ることで見えてきた細胞内相分離現象の時空間デザイン原理
Spatio-temporal design principles of intracellular phase separation○下林 俊典（京都大学 iPS 細胞研究所）
Shunsuke Shimobayashi (CiRA, Kyoto University)

1SGP-6

合成生物学で生きた細胞内の動的構造体を操り、デザインし、理解する
Manipulation, design, and analysis of dynamic intracellular structures with synthetic biology
tools○中村 秀樹 1,2（1 京大・白眉センター, 2 京大・院工学研究科）
Hideki Nakamura1,2 (1Hakubi Center, Kyoto University, 2Grad. Sch. Eng., Kyoto University)
おわりに
Closing Remarks

13:50〜16:20 H 会場（函館市民会館 3F 大会議室）／Room H（Hakodate Citizen Hall 3F Conference
Room）
1SHP 【共催：学術変革領域（B）「筋肉トランススケール熱シグナリング」】“肉”のイマとミライ
The Future of Muscle is Now
オーガナイザー：鈴木 団（大阪大学），大山 廣太郎（量子科学技術研究開発機構）
Organizers: Madoka Suzuki (Osaka Univ.), Kotaro Oyama (QST)
Muscle is one of the main subjects that have been studied extensively in the field of Biophysics. We can now explain
how the force is produced and assembled at all levels of the hierarchy in muscle; single protein molecule, molecularassembly (sarcomere), myofibril, cell (fiber) and tissue. Is the end of muscle study approaching? In this symposium, we
will review the current status with senior researchers, and foresee the future advances with researchers at their early- andmid-carriers who demonstrate originalities and creativities in new methods.
はじめにOpening Remarks– S72 –

1SHP-1

筋収縮・制御機構に関する研究の現在と将来について
About the present and future of research on muscle contraction/regulation mechanism○石渡 信一（早稲田大・理工学術院）
Shin'ichi Ishiwata (Fac. Sci. & Engn., Waseda Univ.)

1SHP-2

高輝度シンクロトロン放射光に照らされる筋肉研究の明るい未来
Rosy future of muscle research illuminated by bright synchrotron radiation X-rays○岩本 裕之（高輝度光科学研究センター）
Hiroyuki Iwamoto (SPring-8, JASRI)

1SHP-3

局所熱パルスによる横紋筋の細いフィラメントの活性化
Microscopic heat pulses induce activation of striated muscle thin filaments○石井 秀弥 1, 福田 紀男 2（1 量研, 2 慈恵医科大・細胞生理）
Shuya Ishii1, Norio Fukuda2 (1QST, 2Dept Cell Physiol, Sch Med, Jikei Univ.)

1SHP-43 次元バイオプリントで作られた和牛ステーキ：未来の肉？
3D-Bioprinted Wagyu Steak: Meat of the future?
○松崎 典弥（阪大院工）Michiya Matsusaki (Grad. Sch. Eng., Osaka Univ.)

1SHP-5

光熱変換を利用した局所熱パルス法による筋肉の熱暴走メカニズムの解明
Thermal runaway in muscles studied using a local heat pulse method○鈴木 団（阪大・蛋白研）
Madoka Suzuki (Inst. Protein Res., Osaka Univ.)
おわりにClosing Remarks
2 日目（9 月 29 日（木））／Day 2（Sep. 29 Thu.）

08:45〜11:15 A 会場（函館アリーナ 武道館 A）／Room A（Hakodate Arena Budokan A）
2SAA NMR で迫る膜とペプチドの生物物理
NMR Studies in Membrane and Peptide Biophysics
オーガナイザー：川村 出（横浜国立大学），相沢 智康（北海道大学）
Organizers: Izuru Kawamura (Yokohama National Univ.), Tomoyasu Aizawa (Hokkaido Univ.)
Biomembranes and peptides have always been important research targets in the field of biophysics. In particular, theirinteractions and dynamic properties have not yet been fully elucidated, and many cutting-edge studies have been
conducted by applying NMR techniques, which provide information at atomic resolution that is difficult to obtain byother spectroscopic methods. In this symposium, researchers in these fields are invited as speakers to discuss the resultsof their research using NMR methods.
はじめにOpening Remarks
2SAA-1

Solid-State NMR spectroscopic approaches to investigate membrane-bound peptide structure
Izuru Kawamura (Grad. Sch. Eng. Sci., Yokohama Natl. Univ.)– S73 –

2SAA-2

Mobility, location, and kinetics of membrane binding and cell entry of peptides by solutionstate 19F and 1H NMR
Emiko Okamura (Faculty Pharm. Sci., Himeji Dokkyo Univ.)

2SAA-3

Sec 非依存性膜蛋白質膜挿入における大腸菌由来糖脂質 MPIase の役割解明
Role of a bacterial glycolipid MPIase in Sec-independent membrane protein integration○野村 薫（公益財団法人サントリー生命科学財団）
Kaoru Nomura (Suntory Foundation for Life Sciences)

2SAA-4

Solid-state NMR measurements of amphotericin B, a natural product that interacts with lipid
bilayers
Yuichi Umegawa (Grad. Sch. Sci., Osaka Univ.)

2SAA-5

Revealing Novel Polymorphs and Cross Propagation for 42-residue Amyloid beta by Solid-state
NMR
Yoshitaka Ishii1,2 (1Tokyo Institute of Technology, School of Life Science and Technology, 2RIKEN, BDR)

08:45〜11:15 B 会場（函館アリーナ 武道館 B）／Room B（Hakodate Arena Budokan B）
2SBA 【共催：新学術領域研究「遺伝子制御の基盤となるクロマチンポテンシャル」】
先端技術と理論で迫るクロマチン機能の理解
Chromatin function as revealed by cutting-edge technique and theory
オーガナイザー：伊藤 由馬（東京工業大学），木村 宏（東京工業大学）
Organizers: Yuma Ito (Tokyo Tech), Hiroshi Kimura (Tokyo Tech)
Recent advance in genomics and imaging technologies have contributed to understanding the function of chromatin forgene regulation beyond the canonical role in genomic DNA packaging. To understand the principles of highly organizedand dynamic chromatin architecture, the integrated approaches using various experimental techniques and theoreticalmodeling are essential. In this symposium, we aim to promote discussion by sharing the latest research on measuringand modeling the biophysical properties of chromatin and the relevance to gene regulation using state-of-the-art
techniques.
2SBA-1Chromatin mobility of X-linked loci and its epigenetic regulation
Yuko Sato1,2, Yuma Ito2, Satoshi Uchino2, Makio Tokunaga2, Hiroshi Kimura1,2 (1IIR, Tokyo Tech, 2Sch.
Life Sci. Tech., Tokyo Tech)

2SBA-2(2Pos303) ヒト生細胞の局所クロマチン動態は細胞周期を通して一定である(2Pos303) Single-nucleosome imaging reveals steady-state motion of interphase chromatin in
living human cells○飯田 史織 1,2, 新海 創也 3, 伊藤 優志 1, 田村 佐知子 1, 鐘巻 将人 2,4, 大浪 修一 3, 前島 一博 1,2（1 遺伝研・ゲノムダイナミクス研究室, 2 総研大・生命・遺伝, 3 理研 BDR, 4 遺伝研・分子細胞工学研究室）
Shiori Iida1,2, Soya Shinkai3, Yuji Itoh1, Sachiko Tamura1, Masato Kanemaki2,4, Shuichi Onami3,
Kazuhiro Maeshima1,2 (1Genome Dynamics Lab., Natl. Inst. of Genet., 2Dept. of Genet., Sch. of Life Sci.,
SOKENDAI, 3RIKEN BDR, 4Mol. Cell Eng. Lab., Natl. Inst. of Genet.)

2SBA-3

DNA の量とクロマチン構造による核のサイズ制御機構
DNA quantity and chromatin structure contribute to nuclear size control in Xenopus laevis○原 裕貴（山口大・理）Yuki Hara (Fac. Sci., Yamaguchi Univ.)– S74 –

2SBA-4(2Pos116) 細胞核内における underwound DNA の蛍光イメージング(2Pos116) Fluorescence imaging of underwound DNA in the cell nucleus○福手 淳平 1,2, 牧 功一郎 1,3, 安達 泰治 1,2,3（1 京大・医生研, 2 京大・院生命科学, 3 京大・院工学）
Jumpei Fukute1,2, Koichiro Maki1,3, Taiji Adachi1,2,3 (1Inst. Life & Med. Sci., Kyoto Univ., 2Grad. Sch.
Biostudies, Kyoto Univ., 3Grad. Sch. Eng., Kyoto Univ.)

2SBA-5(3Pos305) High-resolution mapping of chromatin compaction and dynamics in live cells bylabel-free interference microscopy
Yi-Teng Hsiao, Chia-Ni Tsai, Fasih Bintang Ilhami, Chia-Lung Hsieh (Institute of Atomic and Molecular
Sciences (IAMS), Academia Sinica / Taiwan)

2SBA-6(3Pos301) 細胞内の一分子を三次元でナノレベルの分解能で観察できる「クライオ三次元ナノスコピー」の開発(3Pos301) Cryo-3D Nanoscopy to localize three-dimensional position of individual fluorophorewith nanometer precision in the cell
○成瀬 寛太 1, 松田 剛 1, 溝内 雄太 1, 志見 剛 2, 木村 宏 2, 中田 栄司 3, 森井 孝 3, 松下 道雄 1,
藤芳 暁 1（1 東京工業大学理学院物理学系物理学コース, 2 東京工業大学科学技術創成研究院細胞
制御工学研究センター, 3 京都大学エネルギー理工学研究所）
Kanta Naruse1, Tsuyoshi Matsuda1, Yuta Mizouchi1, Takeshi Shimi2, Hiroshi Kimura2, Eiji Nakata3,
Takashi Morii3, Michio Matsushita1, Satoru Fujiyoshi1 (1Department of physics, Tokyo institute oftechnology, 2Cell Biology Center, Institute of Innovative Research, Tokyo institute of technology,
3Institute of Advanced Energy, Kyoto University)

2SBA-7

新しいクロマチン基盤ユニットである H3-H4 オクタソームのクライオ電子顕微鏡解析
Cryo-electron microscopic analysis reveal a novel structural unit of chromatin○野澤 佳世 1, 滝沢 由政 2, 七種 和美 3, 明石 知子 4, 胡桃坂 仁志 2（1 東京工業大学・生命理工学院, 2 東京大学・定量生命科学研究所, 3 産業技術総合研究所, 4 横浜市立大学・生命医科学研究科）
Kayo Nozawa1, Yoshimasa Takizawa2, Kazumi Saikusa3, Satoko Akashi4, Hitoshi Kurumizaka2 (1Tokyo
Institute of Technology, School of Life Science and Technology, 2The University of Tokyo, Institute forQuantitative Biosciences, 3National Institute of Advanced Industrial Science and Technology, 4Yokohama
City University, Graduate School of Medical Life Science)

2SBA-8

斥力相互作用する溶質混合系における相分離：クロマチン高次構造の視点から
Phase separation in soft-repulsive mixtures: implication for chromatin organization
Takahiro Sakaue, Naoki Iso, Yuki Norizoe (Dep. Phys. Aoyama Gakuin Univ.)

2SBA-91 細胞全ゲノム DNA 複製解析からゲノム三次元構造動態を探る
Unraveling the dynamic 3D genome architecture through single-cell DNA replication profiling○平谷 伊智朗（理化学研究所 生命機能科学研究センター 発生エピジェネティクス研究チー
ム）
Ichiro Hiratani (Laboratory for Developmental Epigenetics, RIKEN Center for Biosystems Dynamics
Research (RIKEN BDR))– S75 –

08:45〜11:15 C 会場（函館アリーナ 武道館 C）／Room C（Hakodate Arena Budokan C）
2SCA 【共催：新学術領域研究 「発動分子科学」】
発動分子科学への若手研究者による挑戦
Tackle “Molecular Engine” by Early-career Researchers
オーガナイザー：小杉 貴洋（分子科学研究所），大友 章裕（分子科学研究所）
Organizers: Takahiro Kosugi (IMS), Akihiro Otomo (IMS)“Molecular Engine”, design of autonomous functions through energy conversion, has bud by the orchestration ofchemists, biologists, and physicists in the last five years. This scientific concept should be passed down to the next
generations for further development. To this end, early-career researchers in various research fields are trying toelucidate the energy conversion mechanism of molecular machines and to design novel ones. In this symposium,
budding researchers who will lead this field related to biophysics in the future will give a talk about their latest exciting
research results by developing cutting-edge technologies and future prospects.
はじめにOpening Remarks
2SCA-1

アクティブマターが示す秩序形成の幾何的設計原理
Geometric design principle for active ordering
Kazusa Beppu1, Yusuke T. Maeda2 (1Appl. Phys., Aalto Univ. Sch. of Sci., 2Phys., Kyushu Univ.)

2SCA-2(1Pos217) Conversion of light-driven outward proton pump rhodopsin into inward proton pump
Maria Del Carmen Marin Perez1, Masae Konno1,2, Himoru Yawo1, Keiichi Inoue1 (1ISSP, Univ. Tokyo,
2PRESTO, Japan Science and Technology Agency)

2SCA-3

フッ素化人工チャネルによる膜間物質輸送
Transmembrane material transport by fluorinated channels○佐藤 浩平（東工大・生命理工）
Kohei Sato (Sch. Life Sci. Tech., Tokyo Tech.)

2SCA-4(3Pos128) 1 分子回転操作実験によって解明されたミトコンドリア由来 ATP 合成酵素における阻害因子 IF1 の一方向制御機構(3Pos128) Unidirectional regulation of ATPase factor 1 in mitochondrial ATP synthase studied
by single-molecule manipulation experiments○小林 稜平 1,2, 上野 博史 1, 岡崎 圭一 2, 野地 博行 1（1 東大・院工・応化, 2 分子研）
Ryohei Kobayashi1,2, Hiroshi Ueno1, Kei-ichi Okazaki2, Hiroyuki Noji1 (1Appl. Chem., Grad. Sch. Eng.,
Univ. Tokyo, 2Inst. for Mol. Sci.)

2SCA-5

1 分子計測・活性測定・タンパク質工学による回転型 V-ATPase の統合的研究
Integrated research on rotary V-ATPase approached by single-molecule observation,
biochemical assay, and protein engineering○大友 章裕 1,2, 飯野 亮太 1,2（1 分子科学研究所, 2 総合研究大学院大学）
Akihiro Otomo1,2, Ryota Iino1,2 (1Institute for Molecular Science, 2The Graduate University for
Advanced Studies)– S76 –

2SCA-6

ミトコンドリア呼吸鎖における熱産生の物理化学的メカニズム
Physicochemical mechanism of heat generation in mitochondrial respiratory chain○武安 光太郎 1,2,3, Namari Nuning4, 中村 潤児 2,5（1 筑波大・数理物質系, 2 筑波大・TREMS, 3 筑
波大・ゼロ CO2, 4 筑波大・院理工情報生命, 5 九州大・I2CNER）
Kotaro Takeyasu1,2,3, Nuning Namari4, Junji Nakamura2,5 (1Fac. Pure and Appl. Sci., Unive. Tsukuba,
2TREMS, Univ. Tsukuba, 3Zero-CO2, Univ. Tsukuba, 4Grad. Sch. Sci. Technol., Univ. Tsukuba, 5I2CNER,
Kyushu Univ.)

2SCA-7キラル液晶の自己組織化ナノ構造を利用した力学センシングと応答速度設計
Mechanical sensor using chiral liquid crystals with self-organized nanostructures and tuning ofmolecular recovery response○久野 恭平 1,2, 宍戸 厚 2, 堤 治 1（1 立命館大・生命科学, 2 東工大・化生研）
Kyohei Hisano1,2, Atsushi Shishido2, Osamu Tsutsumi1 (1Col. of Life Sci., Ritsumeikan Univ., 2Lab. for
Chem. & Life Sci., Tokyo Tech)

2SCA-8Structural stability and dynamics of de novo designed transmembrane peptide barrels
Ai Niitsu1, Jaewoon Jung2, Yuji Sugita1,2 (1Wako Inst., Riken, 2Kobe Inst., Riken)
おわりにClosing Remarks

08:45〜11:15 D 会場（函館アリーナ 多目的室 A）／Room D（Hakodate Arena Multipurpose Room A）
2SDA 先端的ラベルフリーナノポア計測による生物物理学への展開と応用
Innovative label-free nanopore sensing toward biophysical studies and applications
オーガナイザー：山崎 洋人（東京大学），庄司 観（長岡技術科学大学）
Organizers: Hirohito Yamazaki (The Univ. of Tokyo), Kan Shoji (Nagaoka Univ. of Tech.)
The understanding of the biomolecule structural and dynamic properties has provided a plethora of information about
the roles of various molecules, and leads to the development of innovative industrial enzymes and pharmaceuticals.
Among technologies uncovering biological molecules, nanopore sensing has become attractive since it can study single
molecule properties, such as surface charge, molecular size, shape, chain length, chemical structures and so on. In this
symposium, we will organize the session to present the latest nanopore research for biophysics studies and applications.
2SDA-1

Toward broadly accessible, highly scalable solid-state nanopore research
Kyle Briggs (University of Ottawa, Department of Physics)

2SDA-2プローブ型人工細胞システムの応用展開
Application of Probe-Type Artificial Cell Membrane Systems○庄司 観（長岡技大）Kan Shoji (Nagaoka Univ. Tech.)

2SDA-3ナノポアシーケンサと nanoDoc を用いた DNA/RNA 修飾解析
Detection of DNA/RNA modification using nanopore sequencer and nanoDoc○上田 宏生（東京大・先端研・生命データサイエンス）
Ueda Hiroki (Biological Data Science, RCAST, Univ. of Tokyo)

2SDA-4(2Pos290) Nanopore direct determination of DNA methylation and demethylation intermediates
Ping Liu1, Masayuki Honda1, Ryuji Kawano2 (1Department of Food and Energy Systems Science, Tokyo
University of Agriculture and Technology, 2Institute of Engineering, Tokyo University of Agriculture andTechnology)– S77 –

2SDA-5(2Pos315) ATP を検出可能な DNA ナノポアセンサの開発(2Pos315) ATP-detectable DNA nanopore sensor○赤井 大夢, 庄司 観（長岡技術科学大学）
Hiromu Akai, Kan Shoji (Nagaoka University of Technology)

2SDA-6Integrating nanopore sensing and artificial intelligence for multiplex single-virus identification
Akihide Arima (IIFS, Nagoya Univ.)

2SDA-7

Probing the Effect of Ubiquitinated Histone on Mononucleosomes through solid-state nanopores
Hu Rui, Wei Guanghao, Wang Zhan, Qing Zhao (Peking University, School of Physics)

2SDA-8Light Enhanced Solid-state Nanopore for Single Molecule Sensing
Yamazaki Hirohito (The University of Tokyo, Department of Biological Science)

08:45〜11:15 E 会場（函館アリーナ 多目的室 B）／Room E（Hakodate Arena Multipurpose Room B）
2SEA 100nm サイズの分子集団で顕在化する非凡な時空アロステリー
Unique Spatiotemporal Allostery Emerges in 100nm-Sized Molecular Systems
オーガナイザー：成田 哲博（名古屋大学），秋山 修志（分子科学研究所）
Organizers: Akihiro Narita (Nagoya Univ.), Shuji Akiyama (IMS)
Is it possible to explain biological phenomena occurring at the cellular level on the basis of the physicochemical
properties of molecules? Observations focused on the cellular scale provide little information about molecules, while
investigations of molecular structure and dynamics with high spatiotemporal resolution require handling isolated andpurified samples in vitro. However, how and what kind of connections do we need in order to understand biologicalphenomena? In modern life science research, the initial selection of the most suitable model organism has a great impact
on the success or failure of later research. In the same way, the selection of an appropriate spatiotemporal scale isimportant for cutting into the logic of "cross-scale causality". From this perspective, we realize that the smallest unit of
the molecular system that shows some correlation with physiological properties at the tissue or cellular level is
exclusively concentrated in the 100 nm scale (or several hundred molecules). In this symposium, we will examine thespatiotemporal hierarchy of the 100 nm scale from multiple perspectives of biophysics, structural biology, andcomputational science, and discuss strategies for the evolution of correlation into causation.
はじめにOpening Remarks
2SEA-1アクチン線維において顕在化する時空アロステリー
Spaciotemporal allostery in the actin filament○成田 哲博（名古屋大、理学）Akihiro Narita (Grad. Sci, Nagoya Univ.)

2SEA-2

細菌べん毛モーター回転制御機構の理解の進展
Recent understanding of the control mechanism of the bacterial flagellar motor rotation○今田 勝巳（阪大・院理）Katsumi Imada (Grad. Sch. Sci., Osaka Univ.)– S78 –

2SEA-3膜と細胞骨格の動態制御におけるダイナミンのヘクトスケール分子集団のアロステリー変化
Allosteric changes of hecto-scale population of dynamin GTPases provide in dynamic regulationof membranes and cytoskeletons○竹居 孝二, 阿部 匡史, 竹田 哲也, 山田 浩司（岡山大・院医歯薬）
Kohji Takei, Tadashi Abe, Tetsuya Takeda, Hiroshi Yamada (Fac. Med. Dent. Pharma. Sci., Okayama
Univ.)

2SEA-4

夜明けに自律離散する概日時計システム
Autonomous Disassembly of Circadian Clock System at Dawn
○秋山 修志 1,2（1 協奏分子システム研究センター・分子研, 2 総研大）Shuji Akiyama1,2 (1CIMoS, IMS, NINS, 2SOKENDAI)

2SEA-5高速 AFM による 100-nm サイズの分子集団の直接観察
Direct observation of 100 nm-sized molecular systems by high-speed AFM○古寺 哲幸（金沢大・WPI-NanoLSI）
Noriyuki Kodera (WPI-NanoLSI, Kanazawa Univ.)

2SEA-6

長距離アロステリーの物理基盤としてのクーロン結合ネットワーク
Coulomb bond network as a physical basis for long-range allostery○高野 光則（早大・先進理工）
Mitsunori Takano (Grad. Sch. Sci. Eng., Waseda Univ)

2SEA-7(3Pos013) Optineurin の E50K 緑内障変異はオリゴマー粒径を増大させる(3Pos013) The E50K mutation of optineurin increases the oligomer size
○河村 綸太郎 1, 植月 聡也 1, 丹澤 豪人 2, 加藤 貴之 2, 金城 政孝 3, 北村 朗 3（1 北大・院生命科学,
2 阪大・蛋白研, 3 北大・院先端生命）
Rintaro Kawamura1, Soya Uetsuki1, Takehito Tanzawa2, Takayuki Kato2, Masataka Kinjo3,
Akira Kitamura3 (1Grad. Sci. Life Sci., Hokkaido Univ., 2Inst., for Proteins Res., Osaka Univ., 3Fac. Adv.
Life sci., Hokkaido Univ.)
おわりにClosing Remarks

08:45〜11:15 Room F（Hakodate Citizen Hall 1F Main Hall）
2SFA
Japan-US symposium on motor proteins and associated single-molecule biophysics
Organizers: Kumiko Hayashi (Tohoku Univ.), Jakia Jannat Keya (NINS)This is the second symposium between Japan and USA on motor proteins as a continuation of the first one held in 2021
BSJ meeting. Speakers in this symposium are internationally recognized as experts in the field of motor proteins, andtheir associated proteins. We are planning to prepare enough time to have a deep and detailed discussion among the
speakers and audience on these subjects. The symposium topics cover multidisciplinary applications of genetics, bioengineering, bio-chemistry, medical science, and physics, which will give us new insights into motor proteins, as well asinteresting applications of existing single-molecule techniques.
Opening Remarks
2SFA-1Torque Generation Mechanism of F1-ATPase
Hiroyuki Noji, Hiroshi Ueno (Grad. Sch. Eng., Univ. Tokyo)– S79 –

2SFA-2

Regulation of Motors by Microtubule-Associated Proteins
Ahmet Yildiz (University of California Berkeley)

2SFA-3Cholesterol in the cargo membrane amplifies the inhibitory effects of tau on kinesin-1-basedtransport
Qiaochu Li1, James Ferrare2, Jonathan Silver2, John Wilson1, Luis Arteaga-Castaneda1, Weihong Qiu3,
Michael Vershinin4, Stephen King5, Keir Neuman2, Jing Xu1 (1Physics, University of California, Merced,
CA, USA, 2Laboratory of Single Molecule Biophysics, National Heart, Lung and Blood Institute,
National Institutes of Health, Bethesda, MD, USA, 3Physics, Oregon State University, Corvallis, OR,
USA, 4Physics and Astronomy, University of Utah, Salt Lake City, UT, USA, 5Burnett School of
Biomedical Sciences, University of Central Florida, Orlando, FL, USA)

2SFA-4Ultralong-Term, Real-Time Tracking of Single Cargoes in Living Neurons
Sam Peng (Stanford University)

2SFA-5(1Pos137) Plus and minus ends of microtubules respond asymmetrically to kinesin binding by
a long-range directionally driven allosteric mechanism
Huong T Vu1, Zhechun Zhang2, Riina Tehver3, Dave Thirumalai4 (1University of Warwick, 2Harvard
University, 3Denison University, 4University of Texas)

2SFA-6(3Pos143) SLC26 ion transporters act as electricity-driven motor proteins
Tomohiro Shima (Grad. Sch. Sci., Univ. Tokyo)
Closing Remarks

08:45〜11:15 G 会場（函館市民会館 3F 小ホール）／Room G（Hakodate Citizen Hall 3F Small Hall）
2SGA 【共催：新学術領域研究「シンギュラリティ生物学」】
シンギュラリティ生物学を導くイメージング技術
Advanced Imaging Technologies Leading the Way to “Singularity Biology”
オーガナイザー：蛭田 勇樹（慶應義塾大学），渡邉 朋信（理化学研究所）
Organizers: Yuki Hiruta (Keio Unv.), Tomonobu Watanabe (RIKEN)
In order to study the processes that “singularity cells”, considered as minority entities, causing criticality to a multicellular system, comprehensive development of imaging technologies is essential because of its necessary for multilayer and multi-modal observation of the dynamics and functions of the cells and the multi-cellular system. To do theend, the research project “Singularity Biology” have developed a trans-scale microscopy system, AMATERAS, as abasis for imaging. This symposium introduces the microscopy technologies, including AMATERAS, and advanced
probe technologies to visualize singularity phenomena. We hope that this symposium will lead to the creation ofeffective collaboration projects on Singularity Biology.
はじめにOpening Remarks
2SGA-1

シンギュラリティ現象を直接観るトランススケールスコープ AMATERASTrans-scale scope AMATERAS for direct observation of singularity phenomena○市村 垂生 1, 垣塚 太志 2, 橋本 均 1,3, 永井 健治 1,2（1 大阪大学・先導, 2 大阪大学・産研, 3 大阪大学・薬学）
Taro Ichimura1, Taishi Kakizuka2, Hitoshi Hashimoto1,3, Takeharu Nagai1,2 (1OTRI, Osaka Univ.,
2SANKEN, Osaka Univ., 3Grad. Sch. Pharm., Osaka Univ.)– S80 –

2SGA-2

Chemical probes for detecting enzyme activities in living cells with single cell resolution
Mako Kamiya (Dep. Life Sci. Tech., Tokyo Tech.)

2SGA-3(3Pos277) Decoding single-cell transcriptomic phenotypes from cell images enabled by robotic
data acquisition and deep learning
Jianshi Jin1, Taisaku Ogawa1, Nozomi Hojo1, Kirill Kryukov2, Kenji Shimizu3, Tomokatsu Ikawa4,
Tadashi Imanishi2, Taku Okazaki3, Shiroguchi Katsuyuki1 (1BDR, RIKEN, 2Dept. of Mol. Life Sci., Tokai
Univ. Sch. of Med., 3Inst. for Quant. Biosci., Univ. of Tokyo, 4Res. Inst. for Biomed. Sci., Tokyo Univ. of
Sci.)

2SGA-4

局所かつ任意のタイミングで摂動を与える光操作技術 CALI 法とその応用
A light manipulation technology by CALI that provides localized and arbitrarily timed
perturbations○竹本 研（三重大学大学院医学系研究科生化学分野）
Kiwamu Takemoto (Mie University, Graduate School of Medicine)

2SGA-5最小の発光酵素「picALuc」の開発とその応用
Development of the smallest luciferase “picALuc” and its applications○大室 有紀 1, 金 誠培 2, 松井 勇人 1, 叶井 正樹 1, 古田 忠臣 3（1 島津製作所, 2 産総研, 3 東工大・
生命理工）
Yuki Ohmuro1, Sung Bae Kim2, Hayato Matsui1, Masaki Kanai1, Tadaomi Furuta3 (1Shimadzu
Corporation, 2AIST, 3Sch. Life Sci. Tech., Tokyo Tech)

2SGA-6時空間トランススケールイメージングを可能にするケージドルシフェリンの開発
Development of caged luciferin enabling spatiotemporal trans-scale imaging○蛭田 勇樹（慶應大・理工）
Yuki Hiruta (Fac. Sci. Tech., Keio Univ.)

08:45〜11:15 H 会場（函館市民会館 3F 大会議室）／Room H（Hakodate Citizen Hall 3F Conference
Room）
2SHA 【共催：学術変革領域研究（B）「生体分子工学と低物理エネルギーロジスティクスの融合による次世代非侵襲深部生体操作」】
生体分子工学と低物理エネルギーロジスティックスで切り拓く新たな生体操作学
Next-generation biological manipulation pioneered by biomolecular engineering and low-physicalenergy logistics
オーガナイザー：井上 圭一（東京大学），今村 博臣（京都大学）
Organizers: Keiichi Inoue (The Univ. of Tokyo), Hiromi Imamura (Kyoto Univ.)
Optogenetics enabled us precisely and noninvasively manipulate a variety of biological events in vivo such as neuralfiring, gene expression, cellular morphological change and so on. To expand the concept of optogenetics toward
biological events in deep tissue which are difficult by using visible light, further paradigm shift of manipulation
technology is required. To achieve this goal, we are focusing on using photothermal effect, ultrasound and magnetic
field as novel external-field technologies to manipulate biological responses even in deep tissues by combining
biomolecular engineering of new molecular systems and low-physical energy logistics. In this symposium, we will
introduce cutting-edge researches for next generation biological manipulation.
はじめにOpening Remarks– S81 –

2SHA-1

光熱変換を利用した細胞操作に向けた試み
Toward cell manipulation through photothermal conversion○今村 博臣（京都大学生命科学研究科）
Hiromi Imamura (Graduate School of Biostudies, Kyoto University)

2SHA-2高効率光熱変換タンパク質ヒーター創出に向けた分子内熱伝導機構の解明
Elucidation of intramolecular heat transfer mechanism for construction of highly effective
photothermal protein heaters○水野 操（阪大・院理）Misao Mizuno (Grad. Sch. Sci., Osaka Univ.)

2SHA-3

BMI のための高密度皮質脳波電極の開発
Development of high-density ECoG array for BMI○鈴木 隆文 1, 海住 太郎 1, 平田 雅之 1,2（1 脳情報通信融合研究センター（情報通信研究機構、大阪大学）, 2 大阪大学大学院医学系研究科）
Takafumi Suzuki1, Taro Kaiju1, Masayuki Hirata1,2 (1Center for Information and Neural Networks(CiNet), NICT & Osaka Univ., 2Osaka Univ. graduate school of medicine)

2SHA-4

深部神経活動磁場操作に向けた新規分子ツール開発
Development of molecular tools for magnetic manipulation of neural activity in the deep tissue○井上 圭一（東大・物性研）
Keiichi Inoue (Inst. Solid State Phys., Univ. Tokyo)

2SHA-5

生体内磁性粒子を操るための磁気力場の設計と最適化
Design and optimization of magnetic force field for manipulating magnetic particles in living
bodies○関野 正樹 1, 吉岡 輝 1, 中川 桂一 1, 井上 圭一 2（1 東大・工, 2 東大・物性研）
Masaki Sekino1, Hikaru Yoshioka1, Keiichi Nakagawa1, Keiichi Inoue2 (1Grad. Sch. Eng., Univ. Tokyo,
2ISSP, Univ. Tokyo)
おわりにClosing Remarks

13:50〜16:20 A 会場（函館アリーナ 武道館 A）／Room A（Hakodate Arena Budokan A）
2SAP 【共催：新学術研究領域「情報物理学でひもとく生命の秩序と設計原理」】
多細胞系の情報物理学
Information Physics of multi-cellular systems
オーガナイザー：小林 徹也（東京大学），川口 喬吾（理化学研究所），石島 秋彦（大阪大学）
Organizers: Tetsuya J. Kobayashi (The Univ. of Tokyo), Kyogo Kawaguchi (RIKEN), Akihiko Ishijima (Osaka
Univ.)
Physical understanding of multi-cellular systems is the unexplored frontier in biophysics. Sparked by the rapid
advancements in bioimaging, bioinformatics, symthetic biology and so on, multi-cellular systems are becoming apromising target of biophysics. In this symposium, we showcase the attempts to investigate the design principles ofmulti-cellular systems by using or integrating the methods of physics, informatics, and other disciplines.

– S82 –

2SAP-1

多細胞系の情報物理学
Information Physics of multi-cellular systems○小林 徹也（生産研・東大）
Tetsuya J. Kobayashi (IIS, UTokyo)

2SAP-2内皮細胞集団動態と血管新生
Collective endothelial cell migration and angiogenesis○田久保 直子（東京大学アイソトープ総合センター）
Naoko Takubo (Isotope Science Center, The University of Tokyo)

2SAP-3

細胞間コミュニケーションの操作による多細胞パターンのデザイン
Programming multicellular pattern formation with synthetic cell-cell signaling○戸田 聡（金沢大学・ナノ生命）
Satoshi Toda (NanoLSI, Kanazawa Univ.)

2SAP-4(3Pos118) グラフニューラルネットワークによる細胞間の時空間相互作用の推定(3Pos118) Graph-based machine learning reveals rules of spatiotemporal cell interactions intissues
Takaki Yamamoto1, Katie Cockburn2, Valentina Greco2,3, Kyogo Kawaguchi1,4,5 (1Nonequilibrium
Physics of Living Matter RIKEN Hakubi Research Team, RIKEN BDR, 2Department of Genetics, Yale
School of Medicine, 3Departments of Cell Biology and Dermatology, Yale Stem Cell Center, Yale Cancer
Center, Yale School of Medicine, 4RIKEN CPR, 5Universal Biology Institute, The University of Tokyo)

2SAP-5線虫の神経回路における多重情報コードの情報物理学的解析
Analyisis of multiplexed information coding in the nervous system of C.elegans○豊島 有, 松本 朱加, 飯野 雄一（東大・院理・生科）
Yu Toyoshima, Ayaka Matsumoto, Yuichi Iino (Grad. Sch. Sci., Univ. of Tokyo)

2SAP-6器官形態形成プロセスの種間スケーリング
Scaling of organ morphogenetic process between species○森下 喜弘（理化学研究所 生命機能科学研究センター）
Yoshihiro Morishita (RIKEN Center for Biosystems Dynamics Research)
おわりに
Closing Remarks– S83 –

13:50〜16:20 B 会場（函館アリーナ 武道館 B）／Room B（Hakodate Arena Budokan B）
2SBP 【共催：「富岳」成果創出加速プログラム「全原子・粗視化分子動力学による細胞内分子動態の解明」】
富岳を用いた高性能計算による生物物理
High-performance computational biophysics with supercomputer Fugaku
オーガナイザー：松永 康佑（埼玉大学），信夫 愛（理化学研究所）
Organizers: Yasuhiro Matsunaga (Saitama Univ), Ai Shinobu (RIKEN)
Computational approaches are becoming increasingly important in biophysics, not only for simulations but also for thedetailed interpretation of various measurement data. In particular, with the recent launch of modern supercomputers suchas Fugaku, enormous computational resources have become available, and new computational methods and applications
that were not computationally feasible in the past are becoming possible. In this symposium, we invite researchers who
conduct cutting-edge high-performance computations. We discuss current computational research using supercomputers
as well as future directions of computational biophysics.
はじめに
Opening Remarks
2SBP-1

富岳と超並列分子動力学を用いたタンパク質の構造変化、会合と解離
Protein conformational change, association and dissociation observed using Fugaku andmassively parallel molecular dynamics simulations○北尾 彰朗（東工大・生命理工）
Akio Kitao (Scl. Life Sci. Tech., Tokyo Tech)

2SBP-2

Molecular dynamics study of multidrug efflux transporter complex embedded in lipid bilayer:
Role of membrane lipids in the transporter
Keiko Shinoda, Hisashi Kawasaki (AgTECH, GSALS, UTokyo)

2SBP-3(3Pos186) エンベロープ型ウイルス粒子の粗視化シミュレーション：B 型肝炎ウイルス(3Pos186) Coarse-grained Molecular Dynamics Study of Enveloped Virus Particle: Hepatitis B
Virus○浦野 諒, 篠田 渉（岡山大学・異分野基礎研）
Ryo Urano, Wataru Shinoda (Res. Inst. Interdiscip. Sci., Okayama Univ.)

2SBP-4

Binding free energy landscapes of Src Kinase to its inhibitors sampled by two-dimensional
replica exchange molecular dynamics simulations
Ai Shinobu1, Suyong Re1,2, Yuji Sugita1 (1RIKEN, 2National Institutes of Biomedical Innovation, Health,and Nutrition)

2SBP-5REST シミュレーションによるタンパク質やペプチドリガンドの活性制御機構の解析
Applications of REST simulation to understanding regulation mechanism of protein activationand peptide ligands○浴本 亨 1, 山根 努 2, 池口 満徳 1,2（1 横浜市大・生命医, 2 理研・R-CCS）
Toru Ekimoto1, Tsutomu Yamane2, Mitsunori Ikeguchi1,2 (1Grad. Med. Life Sci., Yokohama City Univ.,
2R-CCS, Riken)– S84 –

2SBP-6(1Pos027) Automated Density Extraction of Isomorphous Difference map and Occupancyestimation for Conformer Fitting
Sriram Srinivasa Raghavan1, Florence Tama1,2,3, Osamu Miyashita1 (1RIKEN Center for
Computational Science, Kobe, Japan., 2Institute of Transformative Biomolecules (WPI-ITbM), Nagoya
University, Aichi, Japan., 3Department of Physics, Graduate School of Science, Nagoya University, Aichi,
Japan.)

2SBP-7

スーパーコンピュータ「富岳」を用いたテンプレートマッチング法による生体分子のマルチコ
ンフォメーション解析
Multi-conformational analysis of biomolecule by the template-matching method using thesupercomputer Fugaku○德久 淳師（理研・R-CCS）
Atsushi Tokuhisa (R-CCS, Riken)
おわりにClosing Remarks

13:50〜16:20 C 会場（函館アリーナ 武道館 C）／Room C（Hakodate Arena Budokan C）
2SCP 生体分子の人工設計：タンパク質、RNA、DNA
Design of biomolecules, protein, RNA, and DNA
オーガナイザー：古賀 信康（分子科学研究所），神谷 由紀子（名古屋大学）
Organizers: Nobuyasu Koga (IMS), Yukiko Kamiya (Nagoya Univ.)
The biomolecules, protein, RNA, and DNA, control cell functions. The design technologies for the biomolecules andtheir interactions have been greatly advanced, which made it possible to create a wide range of biomolecules notexisting in nature. In this symposium, each of the biomolecular design geeks will present the basics of the design
technology and latest results. We then discuss about future perspectives to create novel biomolecules.
2SCP-1タンパク質構造の人工設計
De novo design of novel protein structures○古賀 信康（自然・ 生命創成）
Nobuyasu Koga (NINS, ExCELLS)

2SCP-2

Towards the de novo design of binding proteins through beta-sheet folds
Enrique Marcos (Molecular Biology Institute of Barcelona (IBMB-CSIC), Protein Design and ModelingLab)

2SCP-3

Protein engineering for biogeeks; practical examples of structural redesigns of a model protein
and therapeutic antibody designs
Koki Makabe (Grad. Sch. Sci. and Eng., Yamagata univ.)

2SCP-4非環状型人工核酸による天然核酸認識の設計
Understanding the design of acyclic artificial nucleic acids that recognize natural nucleic acids○神谷 由紀子（名大・院工）Yukiko Kamiya (Grad. Sch. Eng., Nagoya Univ.)

2SCP-5Engineering RNA-protein interactions by directed evolution
Keisuke Fukunaga, Yohei Yokobayashi (Nucleic Acid Chemistry and Engineering Unit, OIST)– S85 –

2SCP-6

DNA ナノ構造によるデザインされた人工細胞と人工オルガネラの実現へ向けて
Toward DNA nanostructure-based designed artificial cells and artificial organelles○瀧ノ上 正浩（東工大・情報理工）
Masahiro Takinoue (Sch. Computing, Tokyo Tech)

13:50〜16:20 D 会場（函館アリーナ 多目的室 A）／Room D（Hakodate Arena Multipurpose Room A）
2SDP 金属イオン制御による酵素の動態
Metal-ion regulation of enzyme dynamics
オーガナイザー：織田 昌幸（京都府立大学），森川 耿右（京都大学）
Organizers: Masayuki Oda (Kyoto Prefectural Univ.), Kosuke Morikawa (Kyoto Univ.)
Enzyme function closely correlates with its structural dynamics, and is often regulated by metal-ion binding. In manycases, metal-ions bind to enzymes rather weakly, and induce protein conformations or conformational ensembles. Thisessential structure-function relationship is an attractive but difficult target to be analyzed. The invited speakers present
challenging efforts on enzymes, such as cutinase, RNaseH, and DNAzyme, which are regulated by Ca2+ or Mg2+. We
hope that discussions based on presented biophysical data could facilitate us to understand real dynamic views of metalenzyme interactions.
はじめに
Opening Remarks
2SDP-1

酵素反応における弱い金属イオン結合の意義
Significance of weak metal-ion binding in enzymatic reactions○織田 昌幸（京府大・院生環科）
Oda Masayuki (Grad. Sch. Life Environ. Sci., Kyoto Pref. Univ.)

2SDP-2

PET 分解酵素 Cut190 における弱く結合した Ca2+イオンを介したアロステリック制御
Allosteric regulation of PET-degrading enzyme Cut190 through the weakly bound Ca2+ ion○沼本 修孝（医科歯科大・難研）
Nobutaka Numoto (Med. Res. Inst., Tokyo Med. Dent. Univ.)

2SDP-3

RNaseHI の触媒反応機構：必須金属は 1 個か 2 個か？
Catalytic mechanism of RNaseHI: one metal or two metals?
○森川 耿右（京大・生命科学）
Kosuke Morikawa (Kyoto Univ.)

2SDP-4

エレクトロスプレーイオン化質量分析法による活性型リボヌクレアーゼ HI：RNA/DNA:金属イオン複合体の検出
Active ternary complex of ribonuclease HI: RNA/DNA hybrid: metal ions probed by ESI mass
spectrometry
2 1 大阪○高尾 敏文 1, 安東 友繁 1, 林 潤美 1, Jongruja Nujarin2, 奥村 宜明 1, 森川 耿右 3, 金谷 茂則 （大・蛋白研, 2 大阪大・院工学, 3 京都大・院生命科学）
Toshifumi Takao1, Tomoshige Ando1, Hiromi Hayashi1, Nujarin Jongruja2, Nobuaki Okumura1,
Kosuke Morikawa3, Shigenori Kanaya2 (1Inst. Protein Res., Osaka Univ., 2Grad. Sch. Eng., Osaka Univ.,
3Grad. Sch. Biostudies, Kyoto Univ.)– S86 –

2SDP-5(2Pos026) Structural basis of the significant metal-histidine coordination in E. coli RNase HI
Zengwei Liao1, Takuji Oyama2, Yumi Kitagawa3, Katsuo Katayanagi4, Kosuke Morikawa5,
Masayuki Oda3 (1Grad. Sch. Agri. and Life Sci., the Univ. of Tokyo, 2Faculty of Life and Environ. Sci.,
Univ. of Yamanashi, 3Grad. Sch. Life Environ. Sci., Kyoto Pref. Univ., 4Grad. Sch. Integrated Sci. for Life,
Hiroshima Univ., 5Grad. Sch. Biostudies, Kyoto Univ.)

2SDP-6

Metal interaction and Conformational Changes in HIV-1 Reverse Transcriptase
Rieko Ishima (University of Pittsburgh School of Medicine)

2SDP-7High-resolution and time-resolved insights into an RNA-cleaving DNA catalyst
Manuel Etzkorn1,2 (1Heinrich Heine University Düsseldorf, 2Research Center Jülich)
おわりにClosing Remarks

13:50〜16:20 E 会場（函館アリーナ 多目的室 B）／Room E（Hakodate Arena Multipurpose Room B）
2SEP ダイナミックな翻訳 その開始から終わりまで
Dynamic translation: from initiation to the end
オーガナイザー：丹澤 豪人（大阪大学），楊 倬皓（東京大学）
Organizers: Takehito Tanzawa (Osaka Unv.), Zhuohao Yang (The Univ. of Tokyo)
Translation on ribosomes is a fundamental biological phenomenon that requires strict spatiotemporal regulation andquality control. Since translation is a multi-step reaction, it is necessary to clarify the details of each step in order tounderstand its whole glance. Recently, with advancing and developing analytical methods such as structural analysis,
single molecular imaging, and NGS, it has been uncovered that the translation cycle on ribosomes is regulated in various
ways. In this symposium, we would like to have young researchers from different fields shed light on and discuss the
dynamics of cis/trans-acting translational control on ribosomes from theoretical and experimental perspectives.
はじめに
Opening Remarks
2SEP-1Novel repressive role of eIF4A1 during mTORC1 inhibition
Yuichi Shichino (RIKEN CPR)

2SEP-2(1Pos121) 自由エネルギー地形から探る開始コドン認識機構(1Pos121) Computational Analysis of the Start Codon Recognition Mechanism Based on Free
Energy Landscape○亀田 健 1, 浅野 桂 2,3,4, 冨樫 祐一 1,5（1 立命大 生命, 2 カンザス州立大 生物, 3 広島大 HiHA, 4 広
島大 統合生命, 5 理研 BDR）
Takeru Kameda1, Katsura Asano2,3,4, Yuichi Togashi1,5 (1Coll. Life Sci., Ritsumeikan Univ., 2Div. Biol.,Kansas State Univ., 3HiHA, Hiroshima Univ., 4Grad. Sch. Integ. Sci. Life, Hiroshima Univ., 5RIKEN
BDR)– S87 –

2SEP-3(2Pos109) RNase T2 のリボソームへの結合を介した翻訳阻害機構(2Pos109) Regulation mechanism of translation through the interaction of RNase T2 withribosome○南 篤 1, 丹澤 豪人 2, 楊 倬皓 3, 船津 高志 3, 加藤 貴之 2, 葛山 智久 1,4, 吉田 秀司 5, 小川 哲弘 1,4（1 東大・院農生科, 2 阪大・蛋白研, 3 東大・院薬, 4 東大・CRIIM, 5 大阪医薬大・医）Atsushi Minami1, Takehito Tanzawa2, Zhuohao Yang3, Takashi Funatsu3, Takayuki Kato2,
Tomohisa Kuzuyama1,4, Hideji Yoshida5, Tetsuhiro Ogawa1,4 (1Grad. Sch. Agri. and Life Sci., Univ.
Tokyo, 2IPR, Osaka Univ., 3Grad. Sch. Pharm. Sci., Univ. Tokyo, 4CRIIM, Univ. Tokyo, 5Fac. Med., OsakaMed. Pharm. Univ.)

2SEP-4High-speed AFM visualizes translational GTPase factor pool formed around the ribosomal Pstalk
Hirotatsu Imai1,2, Toshio Uchiumi3, Noriyuki Kodera2 (1Fac. Med., Univ. Ryukyus, 2Nano-LSI,
Kanazawa Univ., 3Fac. Sci., Niigata Univ.)

2SEP-5The mechanical stability of SecM translation arrest
Zhuohao Yang1, Ryo Iizuka2, Takashi Funatsu1 (1Grad. Sch. Pharm. Sci., The Univ. Tokyo, 2Dept. Biol.
Sci., Grad. Sch. Sci., The Univ. Tokyo)

2SEP-6

Attempt to visualize the synthetic polypeptide during translational arrest
Takehito Tanzawa (IPR., Osaka Univ.)

2SEP-7

The final step of protein synthesis; the capture of an unfolded polypeptide by chaperonin GroEL
Kevin Mac Alister Stapleton (Grad. Sch. Frontier BioSci., Osaka Univ.)

2SEP-8

ER Redox shift through the ribosome translation
Ryo Ushioda1,2 (1Fac. of Life Sci., Kyoto Sangyo Univ., 2Inst. for Protein Dynamics, Kyoto Sangyo Univ.)
おわりにClosing Remarks

13:50〜16:20 F 会場（函館市民会館 1F 大ホール）／Room F（Hakodate Citizen Hall 1F Main Hall）
2SFP 【共催：学術変革領域研究（B）「遅延制御超分子化学」】
生物物理学による脳の理解と化学的再生
Biophysical elucidation of neural network and chemical regeneration of neural tissue
オーガナイザー：村岡 貴博（東京農工大学），齋尾 智英（徳島大学）
Organizers: Takahiro Muraoka (Tokyo Univ. of Agriculture and Tech.), Tomohide Saio (Tokushima Univ.)
In recent years, brain science has made remarkable progress. Understanding neural circuits and elucidation of signaltransduction processes at the molecular level are being carried out. Not only neuroscience but also mechanistic
biochemical studies on neural diseases are progressing. Neurodegenerative diseases are one representative example, andthe structure and dynamics of the causative protein are being elucidated at the single-molecule level. Integrating
discussions between biophysical neuroscience and chemical research of the brain should address important unexploredissues such as the precise elucidation of brain function and the development of neuronal tissue regeneration technology.
はじめにOpening Remarks– S88 –

2SFP-1

Phase separation provides a reaction chamber for autophagy progression
Yuko Fujioka (Institute for Genetic Medicine, Hokkaido Univ.)

2SFP-2

蛋白質ミスフォールディング病における蛋白質凝集の分子機構
The molecular mechanism of protein aggregation in protein misfolding disease
Young-Ho Lee1,2,3 (1Research Center for Bioconvergence Analysis, Korea Basic Sci. Inst., Korea, 2BioAnalytical Sci., Uni. of Sci. and Tech., Korea, 3Grad. Sch. of Analytical Sci. and Tech., Chungnam
National Uni., Korea)

2SFP-3

脳神経疾患研究における 1 分子イメージング研究の現状・課題・可能性
Current status, problems, and potential of single molecule imaging studies in neurologicaldisease research○坂内 博子（早大・理工学術院）
Hiroko Bannai (Fac. Sci. Eng., Waseda Univ.)

2SFP-4超分子ペプチドゲルを用いた損傷脳再生
Injured brain regeneration using supramolecular peptide hydrogels○味岡 逸樹 1,2（1 東京医科歯科大学・脳統合機能研究センター, 2 神奈川県立産業技術総合研究所）
Itsuki Ajioka1,2 (1Center for Brain Integration Research (CBIR), Tokyo Medical Dental Univ (TMDU),
2KISTEC)

2SFP-5

相反する匂い価値の脳内表現と神経回路基盤
Representations and circuits for opposing odor values in the brain○風間 北斗（理化学研究所脳神経科学研究センター）
Hokto Kazama (RIKEN Center for Brain Science)
おわりに
Closing Remarks

13:50〜16:20 G 会場（函館市民会館 3F 小ホール）／Room G（Hakodate Citizen Hall 3F Small Hall）
2SGP 物理化学的解析から探るアミロイド・ゲルの構造ダイナミクス
Physicochemical analyses of structural dynamics for amyloid and gel
オーガナイザー：田中 元雅（理化学研究所），真板 宣夫（量子科学技術研究開発機構）
Organizers: Motomasa Tanaka (RIKEN), Nobuo Maita (QST)
Disease-associated proteins often form apparently rigid aggregates such as amyloids and gels. Interestingly, however,recent studies have found that amyloids and gels are not the final dead-end products of proteins, but rather undergo
dynamic structural changes by cellular proteins and environmental factors, which potentially provide great impacts on
cellular phenotypes. However, compared to static structures of amyloids and gels, the details of their dynamic structuralchanges remain poorly understood. In this symposium, we would like to share and discuss the latest findings that clarify
the structural dynamics of amyloids and gels by physicochemical analyses through the development of newtechnologies, and contribute to further advances of the research field.
はじめにOpening Remarks
2SGP-1

Putting prions in context: towards in vivo structural biology using DNP NMR
Kendra King Frederick (UT Southwestern)– S89 –

2SGP-2

The single-particle cryo-electron microscopic analysis of amyloid disaggregation reaction
Takashi Nomura1, Yoshiko Nakagawa1, Yusuke Komi1, Shingo Tamai1,2, Masako Yamazaki1,
Motomasa Tanaka1 (1CBS, RIKEN, 2Biomed. Sci. & Eng., Grad. Sch. of Med. &Dent. Sci., TMDU)

2SGP-3

Critical Jamming and gel rheology of droplet suspensions in living cells
Daisuke Mizuno (Kyushu University)

2SGP-4

レオロジー NMR 法による SOD1 アミロイド形成の多状態その場観察
Multiple-state in situ observation of SOD1 amyloid formation by Rheo-NMR spectroscopy○森本 大智 1, ヴァリンダ エリック 2, 白川 昌宏 1, シェラー ウルリッヒ 3, 菅瀬 謙治 4（1 京大・院
工学, 2 京大・院医学, 3IPF, 4 京大・院農学）
Daichi Morimoto1, Erik Walinda2, Masahiro Shirakawa1, Ulrich Scheler3, Kenji Sugase4 (1Grad. Sch.
Eng., Kyoto Univ., 2Grad. Sch. Med., Kyoto Univ., 3IPF, 4Grad. Sch. Agr., Kyoto Univ.)

2SGP-5

TDP43-LC ドメインの病原性変異と線維形成能の網羅的解析
Comprehensive studies of disease-related mutations on cross-β polymerization of TDP43-LC
domainNobuo Maita1, Yuko Kajino1, Masato Kato1,2 (1National Institutes for Quantum Science and Technology,
2UT Southwestern Medical Center)

13:50〜16:20 H 会場（函館市民会館 3F 大会議室）／Room H（Hakodate Citizen Hall 3F Conference
Room）
2SHP 【共催：学術変革領域研究（A）「マルチファセットプロテインズ」】
マルチファセット・プロテインズへの生物物理アプローチ
Biophysical approach for multifaced protein world
オーガナイザー：渡邉 力也（理化学研究所），太田 元規（名古屋大学）
Organizers: Rikiya Watanabe (RIKEN), Motonori Ota (Nagoya Univ.)
In recent years, our perception of the "protein world" has been expanding and transforming with the discovery of many
aspects that were previously unseen. In this symposium, we would like to discuss the biophysical approaches to clarify
the molecular mechanism and physiological significance of the expanding and changing protein world from a"multifaceted" perspective.
2SHP-1mRNA の翻訳制御を 1 分子解像度で in situ イメージングする
Translational regulation visualized at single-molecule resolution in cells○小林 穂高 1,2（1JST さきがけ, 2 東京大学 定量生命科学研究所）
Hotaka Kobayashi1,2 (1JST PRESTO, 2IQB, The University of Tokyo)

2SHP-2

SARS-CoV-2 nsp1 はホスト翻訳系をどう乗っ取るのか？
SARS-CoV-2 nsp1: how do they hijack the host translation?
○桜庭 俊 1, 謝 祺琳 2, 笠原 浩太 3, 岩切 淳一 4, 河野 秀俊 1（1 量研機構, 2 立命館大・院生命科学,
3 立命館大・生命, 4 東京大・院新領域）
Shun Sakuraba1, Qilin Xie2, Kota Kasahara3, Junichi Iwakiri4, Hidetoshi Kono1 (1Natl. Inst. Quantum
Sci. & Tech., 2Grad. Sch. Life Sci., Ritsumeikan Univ., 3Col. Life Sci., Ritsumeikan Univ., 4Grad. Sch.
Frontier Sci., Univ. Tokyo)– S90 –

2SHP-3神経変性疾患を引き起こすアミロイド線維のクライオ電顕解析
Cryo-EM analyses of the amyloid fibrils causing neurodegenerative diseases○山形 敦史（理化学研究所・生命機能科学研究センター）
Atsushi Yamagata (RIKEN Center for Biosystems Dynamics Research)

2SHP-4(2Pos260) 新規遺伝子の誕生と機能獲得の進化メカニズムに迫るゲノム計算科学：バイオイン
フォマティクスのその先に遺伝子の本質を探求する(2Pos260) How do de novo genes evolve and acquire function?: Computational genomics torevisit the nature of genes beyond bioinformatics○山内 駿 1, 岩崎 渉 1,2（1 東大・院理学系, 2 東大・院新領域）
Shun Yamanouchi1, Wataru Iwasaki1,2 (1Grad. Sch. Sci., Univ. Tokyo, 2Grad. Sch. Front. Sci., Univ.
Tokyo)

2SHP-5Multifaceted view of protein diffusion
Eiji Yamamoto (Dept. Syst. Des. Eng., Keio Univ.)

2SHP-6

生体分子の 1 分子解析とその応用
Single-molecule analysis of bio-molecules and its applications○渡邉 力也（理研・CPR）
Rikiya Watanabe (CPR, RIKEN)
3 日目（9 月 30 日（金））／Day 3（Sep. 30 Fri.）

09:00〜11:30 A 会場（函館アリーナ 武道館 A）／Room A（Hakodate Arena Budokan A）
3SAA 発光・蛍光計測と光学顕微鏡の標準化を目指して
Toward a standardization of luminescence, fluorescence measurements and light microscopy
オーガナイザー：佐々木 章（産業技術総合研究所），近江谷 克裕（産業技術総合研究所）
Organizers: Akira Sasaki (AIST), Yoshihiro Ohmiya (AIST)The quantitative aspect of luminescence, fluorescence measurement and light microscopy is becoming significant. The
challenge now lies in improving the accuracy and precision of the data obtained from such measurements.
Standardization is the way to achieve precise, reproducible and inter-comparable measurement. Improving these will
facilitate the comparison of results between different instruments/institutions and therefore ensure the reproducibility of
results. In this symposium, recent standardization effort in the world (e.g. ISO) will be introduced in addition of leadingedge researches of the related field.
はじめにOpening Remarks
3SAA-1

定量的な蛍光顕微鏡計測に向けて - FCS を用いた顕微鏡ベンチマーク Toward traceable quantitative fluorescence microscopy - Benchmarking microscope using FCS
technique ○佐々木 章（産総研・バイオメディカル）
Akira Sasaki (BMRI, AIST)

3SAA-2

Supporting cellular analysis by quantitative imaging with standards and reference materials
Michael Halter, Ed Kwee, Alexander Peterson, John T Elliott (National Institute of Standards andTechnology (NIST), USA)

– S91 –

3SAA-3細胞を用いた分析・製造分野における細胞形態計測の信頼性向上を目指して〜ISO 標準化活動の紹介
Towards improvement of the reliability of cell morphometry for analysis and manufacturing ofcells - Role of ISO standardization○能見 淑子（千代田化工建設株式会社）
Yoshiko Nomi (Chiyoda Corporation)

3SAA-4(1Pos289) Morphological Analysis of Hydrogel Induced Cancer Stem Cells in Synovial Sarcoma
Model Cells
Zannatul Ferdous1, Masumi Tsuda1,3,4, Jean-Emmanuel Clément3, Jian Ping Gong1,3,6,
Shinya Tanaka3,4,6, Tamiki Komatsuzaki2,3,5, Koji Tabata2 (1Graduate School of Life Science, Hokkaido
University, 2Research Center of Mathematics for Social Creativity, Research Institute for Electronic
Science, Hokkaido University, Sapporo, Japan, 3Institute for Chemical Reaction Design and Discovery(WPI-ICReDD), Hokkaido University, Sapporo, Japan, 4Department of Cancer Pathology, Hokkaido
University Faculty of Medicine, Sapporo, 5Graduate School of Chemical Sciences and Engineering,
Hokkaido University, Sapporo, Japan, 6Global Station for Soft Matter, Global Institution for
Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan)

3SAA-5

Quantification of receptor clustering and activation at the cell surface using correlation andlifetime-based methods
Andrew Harry Albert Clayton (Cell Biophysics, Optical Sciences Centre, Swinburne University ofTechnology, Hawthorn, Australia)

3SAA-6(1Pos288) Size determination of cytoplasmic condensates of optineurin using spatial image
correlation spectroscopy (SICS)
Yuta Hamada1, Masataka Kinjo2, Akira Kitamura2 (1Grad. Sch. Sci. of Life Sci., Hokkaido Univ., 2Fac.
of Adv. Life Sci., Hokkaido Univ)

3SAA-7

絶対発光量計測技術に基づく生物発光反応の量子収率解析とバイオ分析機器標準化
Absolute light measurement for the investigation of bioluminescence quantum yield andstandardization of bioanalysis instruments○丹羽 一樹（産業技術総合研究所物理計測標準研究部門）
Kazuki Niwa (National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial
Science and technology (AIST))

3SAA-8

定量的 in vivo, ex vivo 生物発光イメージング
Quantitative bioluminescence imaging in vitro and ex vivo
○近江谷 克裕 1,2（1 産業技術総合研究所, 2 大阪工業大学）
Yoshihiro Ohmiya1,2 (1AIST, 2Osaka Institute of technology)
おわりにClosing Remarks– S92 –

09:00〜11:30 B 会場（函館アリーナ 武道館 B）／Room B（Hakodate Arena Budokan B）
3SBA 【共催：新学術研究領域「高速分子動画」】
様々な先端的手法で挑む生体分子の構造ダイナミクスの可視化
Visualization of structural dynamics of biomolecules using a variety of advanced techniques
オーガナイザー：梅名 泰史（名古屋大学），清水 伸隆（高エネルギー加速器研究機構）
Organizers: Yasufumi Umena (Nagoya Univ.), Nobutaka Shimizu (KEK)Time-resolved serial femtosecond crystallography (TR-SFX) using X-ray free-electron laser (XFEL) has recently been
established as one of the approaches to obtain structural dynamics of biological molecules. Furthermore, various
biophysical analyses are necessary to understand complicated biological dynamic events properly, and novel methods
have been proposed to obtain more detailed interpretations. The integrated collaborations between these methods and
TR-SFX will take us to visualize biological processes as "molecular movies" in the future. In this session, we will
introduce not only the latest SFX studies but also the various novel approaches to capture dynamic biological events andadvance to understand the biomolecular functions through integrative research.
はじめにOpening Remarks
3SBA-1

ポールシェーラー研究所でのいろいろな実験手法を組み合わせた時分割シリアル結晶学への取り組みTime resolved serial crystallography with various methods at the Paul Scherrer Institut
Takashi Tomizaki, Tsujino Soichiro (Paul Scherrer Institut)

3SBA-2

分子動力学シミュレーションによるタンパク質の構造ダイナミクス研究
Structural dynamics of proteins studied using molecular dynamics simulations○池口 満徳 1,2（1 横浜市大・生命医, 2 理研・計算科学研究セ）
Mitsunori Ikeguchi1,2 (1Grad. Sch. Med Life Sci., Yokohama City Univ., 2R-CCS, RIKEN)

3SBA-3生体分子の動的構造解析のためのマイクロ流体デバイスの開発
Development of microfluidic devices for structural dynamics measurement of biomolecules○真栄城 正寿 1,2,3（1 北海道大学大学院工学研究院, 2JST・さきがけ, 3 高エネルギー加速器研究機構 物質構造科学研究所）
Masatoshi Maeki1,2,3 (1Faculty of Engineering, Hokkaido University, 2JST PRESTO, 3Institute ofMaterials Structure Science, High Energy Accelerator Research Organization (KEK))

3SBA-4

Serial Femtosecond Crystallography reveals structural intermediates during CO-dissociation
process in ba3-type Cytochrome c Oxidase
Swagatha Ghosh1,2, Cecilia Safari1, Rebecka Andersson1, Jonatan Johannesson1, Peter Dahl1,
Eriko Nango3, Rie Tanaka3, So Iwata3, Richard Neutze1, Gisela Brändén1 (1Dept. Chem. and Mol. Bio,
Gothenburg University, Sweden, 2Dept. Appl.Physics, Nagoya University, Japan, 3RIKEN Spring-8
Center, Hyogo, Japan)

3SBA-5光化学系 I I 酸素発生中心における V185 の役割についての QM/MM-MD 解析
QM/MM-MD study of the role of valine 185 in the oxygen-evolving center of photosystem II○庄司 光男 1,2, 宮川 晃一 1, 三嶋 謙二 1, 山口 兆 3, 重田 育照 1（1 筑波大 CCS, 2 さきがけ, 3 大阪大学）
Mitsuo Shoji1,2, Koichi Miyagawa1, Kenji Mishima1, Kizashi Yamaguchi3, Yasuteru Shigeta1 (1CCS,
U.Tsukuba, 2JST-PRESTO, 3Osaka Univ.)– S93 –

3SBA-6

光活性化アデニル酸シクラーゼ OaPAC の動的構造解析による反応機構の解明
Reaction mechanisms of photoactivated adenylate cyclase OaPAC using dynamic structural
analysis○石本 直偉士 1, 梅名 泰史 3, Trampari Sofia2, 辻野 壮一郎 2, 富崎 孝司 2, 朴 三用 1（1 横浜市大・院
生命医科学, 2 ポールシェラー研究所, 3 名大・シンクロトロン光研究センター）
Naito Ishimoto1, Yasufumi Umena3, Sofia Trampari2, Soichiro Tsujino2, Takashi Tomizaki2,
Sam-Yong Park1 (1Grad. Sch. MLS, Yokohama City Univ. / Japanese, 2Paul Scherrer Institute /Switzerland, 3Synchrotron Radiation Research Center, Nagoya University / Japanese)
おわりに
Closing Remarks

09:00〜11:30 C 会場（函館アリーナ 武道館 C）／Room C（Hakodate Arena Budokan C）
3SCA 自主・自発の階層と適応：冗長性を行動力(健康)につなげる分子-細胞-筋-身体-脳連携
Hierarchies of autonomy and spontaneity and adaptation: Molecular-cell-muscle-bodybrain
linkage of redundancy to action (health)
オーガナイザー：跡見 順子（東京農工大学），岩城 光宏（理化学研究所）
Organizers: Yoriko Atomi (Tokyo Univ. of Agriculture & Technology), Mitsuhiro Iwaki (RIKEN)
Fumio Osawa, the founder of the Biophysical Society of Japan, saw the essence of life as "independence andspontaneity. Humans, who are at the mercy of fragmented science, technology, and concepts, have lost sight of the larger
framework for creating independence and spontaneity. This symposium will explore the path to extend the hierarchy oflife's autonomy and spontaneity, in which protein interactions lead to emergence and molecular chaperones lead to
adaptation, from the cell to the human body and mind. This will provide the basis for the creation of new health,industry, and medical science and education.
はじめにOpening Remarks
3SCA-1

Myosin molecular motors convert information into motion
Toshio Yanagida1,2,3,4 (1NICT, 2Grad. Sch. Info. Sci. Tech., Osaka Univ., 3Grad. Sch. Front. Biosci.,
Osaka Univ., 4iFReC)

3SCA-2The network of microtubule integrates the spatial information provided by the actin network
along the cell periphery
Manuel Thery (CEA (French Atomic Energy Reserch Center))

3SCA-3

Molecular mechanisms of the chaperones that assist in the folding of actin and tubulin
Masafumi Yohda (Grad. Sch. Eng., Tokyo Univ. Agr. Tech.)

3SCA-4多細胞動物ヒトの筋適応の素過程：ストレス因子カルシウム、微小管及び分子シャペロン αBクリスタリン
Elementary processes of slow muscle adaptation in multicellular human: calcium, microtubules
and the molecular chaperone αB-crystallin○跡見 順子（東京農工大）Yoriko Atomi (Tokyo University of Agriculture and Technology)– S94 –

3SCA-5

骨格筋幹細胞の活性化・分化と筋再生のサーカディアン制御
Circadian regulation of skeletal muscle stem cell activation differentiation, and muscle
regeneration○朝倉 淳（ミネソタ大学医学部・幹細胞研究所）
Atsushi Asakura (Stem Cell Institute, University of Minnesota Medical School)

3SCA-6

ヒトの不安定な立位姿勢における頭部-体幹部の構造的冗長性の制御
Control of structural redundancy from head to trunk during unstable upright standing in humans○跡見 友章（杏林大学保健学部）
Tomoaki Atomi (Health Sci., Univ. Kyorin)
おわりにClosing Remarks

09:00〜11:30 D 会場（函館アリーナ 多目的室 A）／Room D（Hakodate Arena Multipurpose Room A）
3SDA 【共催：学術変革領域研究（A）「新興硫黄生物学が拓く生命原理変革」】
硫黄のタンパク質科学の最前線
New implications of sulfur in protein science
オーガナイザー：増田 真二（東京工業大学），中林 孝和（東北大学）
Organizers: Shinji Masuda (Tokyo Tech), Takakazu Nakabayashi (Tohoku Univ.)
In recent years, attention has been focused on physiological phenomena involving sulfur, such as finding supersulfide
molecules consisting of multiple sulfur atoms in mammals, including humans. In this symposium, six presentersintroduce their recent results of structure-function relationships of sulfur-related proteins. We would like to discuss how
biophysics can be applied to physiological phenomena involving sulfur.
はじめにOpening Remarks
3SDA-1

Mechanism of sulfide/supersulfide sensing in bacteria
Shinji Masuda (Grad. Sch. Life Sci. & Technol., Tokyo Inst. Tech.)

3SDA-2Intramolecular disulfide bond switches enzymatic activity of SOD1
Shinya Tahara1, Kousuke Yamazaki1, Takumi Ohyama1, Kunisato Kuroi2, Takakazu Nakabayashi1(1Grad. Sch. Pharm. Sci., Tohoku Univ., 2Dept. Pharm. Sci., Kobe Gakuin Univ.)

3SDA-3

Reaction mechanism of tRNA sulfur modifying enzyme using a cofactor iron-sulfur cluster
Min Yao (Fac. Adv. Life Sci., Hokkaido Univ.)

3SDA-42 つの異なるタイプの PLP 依存型システイン脱硫酵素と基質 L-システインおよび阻害剤との反応
Actions of two distinct types of PLP-dependent cysteine desulfurase enzymes with substrate
L-cysteine and inhibitors○藤城 貴史（埼玉大学大学院理工学研究科）
Takashi Fujishiro (Grad. Sch. Sci. Engeneer., Saitama Univ.)– S95 –

3SDA-5Structural and functional analyses of E. coli SufBCD complex involved in iron-sulfur clusters
biogenesis
Kei Wada1,2, Yoshikazu Tanaka3, Yasuhiro Takahashi4 (1Department of Medical Sciences, University ofMiyazaki, 2Frontier Science Research Center, University of Miyazaki., 3Graduate School of Life Sciences,
Tohoku University, 4Graduate School of Science and Engineering, Saitama University)

3SDA-6アミノ酸とナノカーボンの相互作用：物理吸着およびシステインの化学反応
Interactions of carbon nanomaterials with amino acids: physical adsorption and chemical
reaction with cysteine○平野 篤（産総研・ナノ材料）
Atsushi Hirano (NMRI, AIST)
おわりに
Closing Remarks

09:00〜11:30 E 会場（函館アリーナ 多目的室 B）／Room E（Hakodate Arena Multipurpose Room B）
3SEA 【共催：NEDO ムーンショット型研究開発事業】
生物を利用したゼロエミッション・CO2 資源化技術の可能性
Potential of zero-emission and CO2-utilizing biotechnologies
オーガナイザー：加藤 創一郎（産業技術総合研究所），近藤 英昌（産業技術総合研究所）
Organizers: Souichiro Kato (AIST), Hidemasa Kondo (AIST)“Zero-emission”, which will reduce the emission of greenhouse-gases such as CO2, CH4, and N2O to mitigate climatechanges, are being tackled internationally. The technologies attracting attention in recent years are physicochemical
methods such as Direct Air Capture (DAC) and CO2 Capture and Storage (CCS). Considering the mitigation ofgreenhouse gases generated from agriculture and the utilization of CO2, it is necessary to develop new technologies thatutilize specific abilities of living organisms. In this symposium, research projects for innovative zero-emission and CO2utilizing biotechnologies conducted by Moonshot Research & Development Program are introduced.
はじめにOpening Remarks
3SEA-1

微生物電気化学を活用した二酸化炭素資源化技術
CO2 utilization technologies based on microbial electrochemistry○加藤 創一郎（産総研・生物プロセス）
Souichiro Kato (BPRI, AIST)

3SEA-2

気相微生物反応Microbial gas-phase reaction○堀 克敏（名古屋大学大学院工学研究科）
Katsutoshi Hori (Grad. Sch. Eng., Nagoya Univ.)

3SEA-3

資源循環の最適化による農地由来の温室効果ガスの排出削減
Mitigation of greenhouse gas emissions from agricultural lands by optimizing nitrogen andcarbon cycles○南澤 究（東北大・院生命）
Kiwamu Minamisawa (Graduate School of Life Sciences, Tohoku University)– S96 –

3SEA-4

土壌団粒構造と微生物
Soil aggregate structure and microorganisms○和穎 朗太（農研機構・農業環境部門）
Rota Wagai (NARO/NIAES)

3SEA-5

ウシルーメンマイクロバイオーム制御による消化管メタンの削減をはかる新しい家畜生産システム開発に向けて
Toward a new livestock production system to reduce enteric methane through controlling
bovine rumen microbiome○小林 泰男（北海道大学大学院農学研究院）
Yasuo Kobayashi (Research Faculty of Agriculture, Hokkaido University)

3SEA-6

牛ルーメンからのメタン低減に向けた微生物利用の可能性
Potential microbial target for mitigating enteric methane production in the rumen of cows
○真貝 拓三（国立研究開発法人 農業・食品産業技術総合研究機構 畜産研究部門）
Takumi Shinkai (Institute of Livestock and Grassland Science, National Agricultural and Food
Research Organization)
おわりにClosing Remarks

09:00〜11:30 F 会場（函館市民会館 1F 大ホール）／Room F（Hakodate Citizen Hall 1F Main Hall）
3SFA
クライオ電子顕微鏡が魅せる生命の未知なる動的なメカニズム
Unexpected dynamic mechanisms of life uncovered by Cryo-EM
オーガナイザー：濡木 理（東京大学），西増 弘志（東京大学）
Organizers: Osamu Nureki (The Univ. of Tokyo), Hiroshi Nishimasu (The Univ. of Tokyo)
Recent outstanding development of single particle analysis of cryo-EM allows high-resolution structure determinationsof huge and flexible supramolecular complexes, which have been never available. In this symposium, we will present
and discuss on current topics of unexpected dynamic molecular and cellular mechanisms of protein and nucleic acidsupramolecular complexes involved in various life phenomena.
3SFA-1

Structure-function relationship of pump-like cation channelrhodopsins
Koichiro Kishi1, Yoon Seok Kim2, Masahiro Fukuda1, Masatoshi Inoue2, Tsukasa Kusakizako3,
Peter Wang2, Toshiki Matsui1, Keitaro Yamashita4, Takashi Nagata5, Masae Konno5, Tomoko Uemura6,
Kehong Liu6, Mikihiko Shibata7, Norimichi Nomura6, So Iwata6, Osamu Nureki3, Keiichi Inoue4,
Karl Deisseroth2, Hideaki Kato1 (1Komaba Inst. Sci., Grad. Sch. Arts. Sci., Univ. Tokyo, 2Stanford Univ.,
3Grad. Sch. Sci., Univ. Tokyo, 4MRC, 5ISSP, Univ. Tokyo, 6Grad. Sch. Med., Kyoto Univ., 7Kanazawa
Univ.)

3SFA-2

III-E 型 CRISPR-Cas7-11 エフェクター複合体の立体構造と分子改変
Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex○西増 弘志（東京大学）
Hiroshi Nishimasu (The University of Tokyo)– S97 –

3SFA-3(2Pos003) クライオ電子顕微鏡による高分解能解析によって明らかになってきた二成分毒素の膜透過機構(2Pos003) High-resolution Cryo-EM analysis reveals the mechanism of binary toxintranslocation○山田 等仁 1, 杉田 征彦 2,3, 野田 岳志 2, 津下 英明 1（1 京都産業大学 大学院生命科学研究科, 2 京
都大学 微細構造ウイルス学分野, 3 京都大学 白眉センター）
Tomohito Yamada1, Yukihiko Sugita2,3, Takeshi Noda2, Hideaki Tsuge1 (1Graduate School of Life
Science, Kyoto Sangyo University, 2Laboratory of Ultrastructural Virology, Institute for Life and Medical
Sciences, Kyoto University, 3Hakubi Center for Advanced Research, Kyoto University)

3SFA-4

IscB–ωRNA 複合体による RNA 依存性 DNA 切断の構造基盤と Cas9 への進化的洞察
Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9○加藤 一希 1, 岡崎 早恵 1, Kannan Soumya2, Zhang Feng2, 西増 弘志 1（1 東大・先端研, 2MIBR,MIT）Kazuki Kato1, Sae Okazaki1, Soumya Kannan2, Feng Zhang2, Hiroshi Nishimasu1 (1RCAST, Univ.
Tokyo, 2MIBR, MIT)

3SFA-5

ミトコンドリアのリボソームの成熟過程から翻訳開始過程に至る構造解析
Structural analysis of the late assembly states of mitochondorial ribosome to the translation
initiation
Yuzuru Itoh1,2, Anas Khawaja3, Joanna Rorbach3, Alexey Amunts2 (1Dept. BioSci., Grad. Sch. Sci.,
Univ. Tokyo, 2SciLifeLab, DBB, Stockholm University, 3Karolinska Institutet)

3SFA-6膜タンパク質と非翻訳 RNA の分子機構の構造基盤
Structural basis for molecular mechanisms of membrane proteins and non-coding RNA○濡木 理（東京大学・院理）Osamu Nureki (Grad. Sch. Sci., Univ. Tokyo)

09:00〜11:30 G 会場（函館市民会館 3F 小ホール）／Room G（Hakodate Citizen Hall 3F Small Hall）
3SGA 自己組織化で超分子生体膜を創る: 材料科学と生物物理学の接点
Creation of supramolecular biomembrane by the bottom-up self-assembly:Where material science
meets biophysics
オーガナイザー：安原 主馬（奈良先端科学技術大学院大学），森垣 憲一（神戸大学）
Organizers: Kazuma Yasuhara (NAIST), Kenichi Morigaki (Kobe Univ.)
In biological systems, unique material properties of the membrane play central roles. The two-dimensional fluid andcompartmentalization are essentially important in a variety of biological functions such as signal transduction andenergy conversion. Bottom-up approaches based on the self-assembly of materials are promising to reproduce theunique membrane structures and functions, providing insights into the machinery of the biological membrane andenabling to exploit applications in real-life. This symposium will introduce unique studies to create novel artificial
biomembranes using not only conventional phospholipids but also synthetic polymers, nanoparticles, and their hybrids
to explore the interface between biophysics and material science.
はじめに
Opening Remarks
3SGA-1

ポリマー化脂質膜と天然脂質膜からなるパターン化人工膜
Micropatterned model membrane composed of polymerized and natural lipid bilayers○森垣 憲一 1,2（1 神戸大・バイオシグナル, 2 神戸大・院農学）
Kenichi Morigaki1,2 (1Biosignal Res. Cen., Kobe Univ., 2Grad. Sch. Agrobio., Kobe Univ.)– S98 –

3SGA-2

メカノクロミック生体膜を用いたペプチドー脂質相互作用の検出
Mechanochromic biomembranes for studying peptide-lipid interactions○杉原 加織（東大・生研）
Kaori Sugihara (IIS, Univ. Tokyo)

3SGA-3

多価不飽和脂質によって形成される脂質ドメイン
Lipid domains generated by polyunsaturated lipids
ゴー メルヴィン ウェイ シェン, ○手老 龍吾（応化生命系・豊橋技科大）Melvin Wei Shern Goh, Ryugo Tero (Dept. Appl. Chem. Life Sci., Toyohashi Univ. Tech.)

3SGA-4

リキッドマーブル：粒子膜で安定化された液滴
Liquid marble: Droplet covered by particulate membrane○藤井 秀司（大阪工業大学）
Syuji Fujii (Osaka Institute of Technology)

3SGA-5(2Pos188) DNA ゲル骨格が決定する人工細胞の力学特性(2Pos188) Cytoskeletons of self-assembled DNA regulate the mechanical properties of artificialcells○増田 和俊 1, 大野 風優 2, 柳澤 実穂 1,2（1 東京大学教養学部, 2 東京大学大学院総合文化研究科）
Kazutoshi Masuda1, Fuyu Ohno2, Miho Yanagisawa1,2 (1College of Arts and Sciences, The University ofTokyo, 2Graduate school of Arts and Sciences, The University of Tokyo)

3SGA-6

生体膜表面を模倣した高分子自己集合体
Self-assembled polymer aggregates with mimetic cell membrane surface○遊佐 真一（兵庫県立大学大学院工学研究科応用化学専攻）
Shin-ichi Yusa (Department of Applied Chemistry, Graduate School of Engineering, University of
Hyogo)

3SGA-7

合成高分子によって形成される最小モデル膜としての脂質ナノディスク
Lipid nanodisc as a minimal model membrane formed with synthetic polymers○安原 主馬 1,2（1 奈良先端大院・物質, 2 奈良先端大・デジタルグリーンイノベーションセン
ター）
Kazuma Yasuhara1,2 (1Div. Mat. Sci, Nara Inst. Sci. Tech., 2Ctr. for Digital Green-innovation, Nara Inst.
Sci. Tech.)
おわりに
Closing Remarks– S99 –

09:00〜11:30 H 会場（函館市民会館 3F 大会議室）／Room H（Hakodate Citizen Hall 3F Conference
Room）
3SHA 【共催：学術変革領域研究（A）「超越分子システム」 / 学術変革領域研究（B）「SPEED」】
高次機能性分子システム〜創る方法の解明に向けて〜
Construction of Higher-ordered Molecular Systems - How to Create Them?
オーガナイザー：松浦 友亮（東京工業大学），川野 竜司（東京農工大学），鈴木 雄太（京都大学）
Organizers: Tomoaki Matsuura (Tokyo Tech), Ryuji Kawano (Tokyo Univ. of Agriculture and Tech.), Yuta
Suzuki (Kyoto Univ.)
We would like to take place a joint symposium collaborated with the “Cell-free molecular system” (Grant-in-Aid forTransformative Research Areas (A)) and the “SPEED” (Grant-in-Aid for Transformative Research Areas (B)). Thissymposium aims to shed light on the bottom-up construction of the cell-free system and the superior protein engineering
by evolution and design.
はじめに
Opening Remarks
3SHA-1

Constructing an in vitro gene screening system for membrane proteins and its application
Tomoaki Matsuura (ELSI, Tokyo Tech)

3SHA-2

人工金属酵素を用いた触媒システムの構築
Artificial enzymes towards systems catalysis○岡本 泰典（東北大・学際研）
Yasunori Okamoto (FRIS, Tohoku Univ.)

3SHA-32 次元ナノ材料界面を利用した高感度バイオセンサの開発
Development of Highly Sensitive Biosensor Using Two-Dimensional Nanomaterial Interface○早水 裕平（東工大・物質理工）
Yuhei Hayamizu (Sch.Mater, Tokyo Tech)

3SHA-4分子進化によるタンパク質集合体の構築
Directed evolution of protein assembly○寺坂 尚紘 1, 菅 裕明 1, Hilvert Donald2（1 東京大・院理学, 2Laboratory of Organic Chemistry, ETH
Zurich）
Naohiro Terasaka1, Hiroaki Suga1, Donald Hilvert2 (1Grad. Sch. Sci., The Univ. of Tokyo, 2Laboratoryof Organic Chemistry, ETH Zurich)

3SHA-5人工細胞膜システム：デノボ設計ナノポアの構築
Artificial Cell-membrane system: the construction of de novo nanopores○川野 竜司（東京農工大学 工学研究院 生命工学専攻）
Ryuji Kawano (Dept. Biotech&Life Sci., Tokyo University of Agriculture and Technology)

3SHA-6

合理設計による機能性タンパク質集合体の構築
Rational design of protein assembly○鈴木 雄太（京大・白眉）
Yuta Suzuki (Hakubi Center, Kyoto University)– S100 –