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11 月 12 日（月）11:00 -

受付開始

12:30 - 12:40

開会の辞

12:40 - 14:40

口頭発表（O-1～10）

14:40 - 15:00

休憩

15:00 - 16:00

特別講演

16:00 - 17:30

ポスター発表（奇数番号）

18:00 -

懇親会

11 月 13 日（火）9:30 - 12:30

シンポジウム12:30 - 13:30

昼休み

13:30 - 15:00

ポスター発表（偶数番号）

15:00 - 15:10

休憩

15:10 - 17:10

口頭発表（O-11～20）

17:10 - 17:25

休憩

17:25 - 18:00

総会、表彰式、閉会の辞 2 —



発表演題および講演時間
特別講演

11 月 12 日（月）15:00 - 16:00

Diversity and evolution of wood decay systems in saprotrophic andmycorrhizal Agaricomycetes (mushroom-forming fungi)
Professor David Hibbett (Clark University)

シンポジウム11 月 13 日（火） 9:30 - 12:30「植物と菌類の相互作用」
9:30-10:15
S-1#2!3<i\dl=Sue{yb:kC_^    f]hd~anrX \d4Qhdm58inr/*&(                          |Z `
10:15-11:00
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11:00-11:45
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11:45-12:30
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12:40 O-1

11  12  12:40 - 14:40

ĠõÑñïčåÈc¾éèchÊÏÐkGÛàÌĎ~F 1ģKenneth Bruno2ģSue Karagiosis2ģDavid Culley2ģShuang Deng2ģJames Collet2ģÚ
P¨} 1ģ³¬ó} 1ģ¿ô¦ 1ģScott Baker2ģÓÒėL 1 ġ1 ÐìÛDÏÊ9B,+2 æ
s6*80&5E+!"+2sáÛàĢ

12:52 O-2
ğõěĈS}þ°9B<E/E*=02#8*+1;đÖV  e¦} ĀÃ _±} H mú ġ {ĖD\âÛ ČìÛĢ

13:04 O-3

Aspergillus nidulans %?&28?4CÏhēIĈS}ºðþ°    [¯ć 1ģÔW|Ċ 1ģÅĎuą 1ģNÜÁx 2ģãö 2ģ÷»_ 2ģĎ¬p 1ģ
N 1 ġ1 w{DÏÊÏlD2 M{DĖDĆĢ

13:16 O-4Aspergillus nidulans septin interactions and post-translational modifications.
Shunsuke Masuo, Yainitza Hernández-Rodríguez and Michelle Momany (Dept. of Plant Biology,
Univ. of Georgia)

13:28 O-5

Characterization of Stress Granules in Aspergillus oryzae
Hsiang-Ting HUANG, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The
Univ. of Tokyo)

13:40 O-6!*'.72>/(ZĐü cag1 ĈS}Tup1 Øi/C6&ă)E3
¬f [ģjĄāÄ, Ém¸ ġÝÒÙá{DÏÊĂÇĢ
13:52 O-7

ĠõÐÏ hydrophobin RolA rTùĘĒØOUÑº¶þ°Òĉ½ 1ģÒJª 1ģ{Ĝ§} 1ģGeYN 1ģĞ¹ 2,3ģ]·ā 4ģ©Òß 4ģĕċ£ 1,3ġ1 ®d{ĖDÏÊÐµbģ2 ČìÛDx×ģ3 ®d{Dª­Ûģ4 ®d{Dz[ÛĢ14:04 O-8

Trichoderma reesei ,A?E-ĞÏÐyÕ²
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1

2

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14:16 O-9*/(çû

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DNA ÛàĢ¤ý GH 8:@E endo--1,3-'A$4E-

v«úFģďĎ¼ ġÏÛĢ

14:28 O-10 ,A?E-ĈS}ÖÍ`q} ClbR ØOUÑq}¢ê
t¼ëî 1,2ģÿXÀ 1ģÈÿĚF 1ģfag 1 ġ1 Ĕ{ĖDÏÎÞģ2 ¥«ø¡òRË^Ûàn PDĢ

 4 —

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O-11O-20

11  13  15:10 – 17:10

15:10 O-11

Co-regulation of A. nidulans cellulase genes by transcription factors McmA and ManR
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo KobayashiĤGraduate Sch.
of Bioagric. Sci., Nagoya Univ.ĥ

15:22 O-12

AtrR  Aspergillus fumigatus #=)-1>A=oéđSÜÔ`

~|¿ 1ĦËÃ[ 2Ħõj~â 2Ħ¤ă 1Ħ¬Č 2ĦLsdH 1Ĥ1 ¯f~ĜĈ@×ÒØ¸bĦ2 gö~@Þô.?/Aĥ

15:34 O-13

Neurospora crassa #=)-1>A=×oěa erg đSĀ`CzĦJMāGĦġ»¾uĦBà§Ħø®ÞĤ¯Ç~Ĝ@×tĥ

15:46 O-14

Chaetomium globosum ÑÒ×ođSáæÓK½R

ĂØÒđSÜÔ`ª×ÀĚDEÅOĦà\cBĒĦ­¬þĦLkÙĦUøĎ~ĦėlinĦÌĉąKĤĞÝ~@÷ĥ

15:58 O-15

!4ÛôK^{Sďé¡I8<'/!3eoÒ×ØĀ¬ġĦ±¢¦ĦÙÇĦĘÙúG ĤÕázá@'9%=6!$>,Aĥ

16:10 O-16

Aspergillus fumigatus ¤ýÛjwë¼ðÿ°ĔMĠÄĦ~óÙêĦġ»µĦL5Mċ Ĥgö~@Þô.?/Aĥ
16:22 O-17

!4Ûô HsbA º/?7&Ć Mohsb1ĦMohsb2 ÛjĚDyl¿@û®ĢĖÄ (×Òá)

16:34 O-18

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ßv^ečåĐ_ìïr«ČùĚDÊÙmî1)Ħyl ¿2)ĦFYG1)ĤNē~Ĝ@×Ö1ĥ×Òá2)ĥ

16:46 O-19!0)ģ£Ûô 1.0 Mb ³ñW(A3 AF Âê×ođS&;-/Apj¿î 1ĦĊø¥XÕ 1Ħ

¬X¦ 1ĦęÏg¨ 1ĦâP 1Ħ¬i 2ĦãZuH 3Ħ´· 1Ĥ1 q~Ĝ@×tĈĦ2 ~@ĈĦ3 Ġ~@Ĉĥ

16:58 O-20

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P-3454

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P-5

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P-6

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P-9

Aspergillus nidulans 6)-6*:Ò`đ@
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Ģ1 nu|;ÒÍÒb;2 Cu;ē;Ăģ

P-10

Aspergillus nidulans  ugeB ĆK{»ðüµÕAğIĥËčhā 1EÝÆo 2ãõ 2ö¼U 2č±e 1ÒÍÒb;2 Cu;ē;Ăģ

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P-11

Aspergillus fumigatus 4*14-:$ðÓù×õJvØÆ}Ptöć·úkñćEÐ´nćÕäćå²Vćú¢ić~EąmsćBsĆ


P-12

Ö¼á Aspergillus nidulans -1,3-68aù×õJvrÇ§æ¾lé¦
bèh 1ćÔã¨ 2ć;¡½ 2ćþ÷ 1,2 ą1 ¤\s9£Îć2 ¤\sÿò9Á»Â«YĆ

P13

Ö¼á Aspergillus nidulans -68aù×õJvÏq§ Congo red _áÖ Congo red cË
12Ôã¨ , bèh , þ÷

P14

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P-15

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P-16Ąá MAP ,:% AoFus3

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1Ìâsìć@}¹;ćÖzgür Bayram ćOliver Valerius1ćGerhard H. Braus1ć\[ą¤sÿ9ò1

ÁÒ9Áć ()88swĆ

P-17

P-18

Ąá A. oryzae ¸áyÙÜQÅÚćLKkć@}¹;ć\[ą¤sÿ9òÁÒ9ÁĆĄá8+!*:"$ýó Aip '8/ï°Ýé¦¥{îIć®`ç±öćàüĀć~ wć\

P-19

Ąá6
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P-20

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P-21

SÁû°¬é¦] 2ćă=Ñ 2ćsªOö 2ćMichelle Momany3ćå²V 1 ą1 Bsÿ9òć<føĂć3 #3:#sĆÖ¼á Aspergillus nidulans Ußù³ Saccharomyces cerevisiae LAS17 6&7
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Ąá A. oryzae ôÈ:*1#:ýóõJv°Ýé¦ÄĀAćàüĀć\[ ą¤sÿ9òÁÒ9ÁĆ 7 —

P-23

Characterization of Stress Granules in Aspergillus oryzae
Hsiang-Ting HUANG, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The Univ. ofTokyo)

P-24

Ģġ÷WÏzÌÔ×ĊIz¾óÿµćDĘĥ·ÒFĥYÒÆĥÄĥæTĈė ģ³ĆvĖ:Ï]Ĥ

P-25

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P-27

Discovering fundamental mushroom developmental genes.
Arend F. van Peer, Yuichi Sakamoto (IBRC)

P-28¡z÷"-(6#

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P-29

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P-30

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P-31

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P-32

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P-34

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P-35

Aspergillus aculeatus Ø´ZÆw -glucosidase fÏ¡èĆQJ, ĆTÅ, ÍĆĞ@, h^l ģėĘ>ÔÓåĤ

P-36

GH3 -$;'(.?,-6:#16:3(.?,»É¡©ÎĈD, ĆTÅ, ÍĆĞ@, h^l

P-37(ėĘ>ÔÓå)Aureobasidium pullulans Ø´- L -:536:3(.?,đÀ Pichia pastoris ġÚÑđíaÛ¡ĉ
µ×àÈĥ×@ù ģċ>

P-38

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P-40

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P-41

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P-42

Ģü Aspergillus oryzae ĕ7</?,ĎOĊYă¶ĐGL 1ĥèXČĜ 1ĥi[ì 2ĥáG@ 2ĥ­Â_ 2ĥÈ 1
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P-43

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P-44

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P-45

ligD ĎOã}ÖĢü hapX ã}¸gă¶{×ā@ď 1ĥ×ģnĔĤ 2ĥb±\ÿ 2ĥÁVH 1ĥ`þĝ~ 1 ģ1 pxĘ>ċ2 Õå¦ñ+>Ġ¦+Ĥ 9 —

P-46

¹ A. oryzae $$9¡­±(°kS¼)

P-47

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P-48

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P-49

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Trichoderma reesei のセルラーゼ生産に関与するシグナル伝達関連タンパク質の解析
日下秀行，古川隆紀, 深谷英嗣, 志田洋介，小笠原 渉

P-51

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（長岡技大・生物）

Trichoderma reesei のセルラーゼ生産に関与する MFS トランスポーターの機能解析
古川隆紀, 志田洋介, 小笠原渉（長岡技大・生物）

P-52

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Aspergillus aculeatus clbR ¸b¶\na+¼My¼¤4|¼¤·'¼\D;E (²^R³%)P-53

P pH "!&:d

Wm5S¬¼µ[lo¼¯*2U¼kn¼XrKT ºGR³I§%wu»

P-54

Co-regulation of A. nidulans cellulase genes by transcription factors McmA and ManR
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo KobayashiºGraduate Sch. of Bioagric.
Sci., Nagoya Univ.»

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Diversity and evolution of wood decay systems in saprotrophic andmycorrhizal Agaricomycetes (mushroom-forming fungi).
David Hibbett, Clark University, Worcester MA 01610 USA.

The Agaricomycetes includes over 20,000 described species of mushrooms, polypores,
puffballs and other macrofungi. This diverse assemblage has both saprotrophic(decayer) and biotrophic (symbiont or pathogen) modes of obtaining carbon nutrition.
Saprotrophic Agaricomycetes include the vast majority of microorganisms that are able
to decay wood (a major pool of organic carbon), as well as decomposers of leaf-litter andother substrates. Among the wood-rotting species, two principal modes of decay occur:
white rot, in which both lignin and cellulose are decomposed, and brown rot, where
cellulose is attacked but lignin remains as a polymeric residue. The most common formof biotrophy is ectomycorrhizal symbiosis (ECM), although the Agaricomycetes also
contain plant pathogens, mycoparasites, and mutualistic partners of insects (such as
attine ants).
In collaboration with the DOE Joint Genome Institute and an international
consortium of partners, we are using phylogenomic approaches to understand thepattern and mechanisms of switches between nutritional modes in Agaricomycetes. To
reconstruct the evolution of wood decay mechanisms, we generated and analyzed
complete genomes of twelve species of Agaricomycotina, including six white rot, fivebrown rot, and one mycoparasitic species, and we combined these with existing genome
sequences of 19 species. We found that all white rot genomes encode multiple copies oflignin-degrading class II fungal peroxidases (POD), which have been repeatedly lost inbrown rot lineages, as have genes encoding many other decay-related oxidoreductases
and enzymes attacking crystalline cellulose. The Agaricomycete Laccaria bicolor and theAscomycete Tuber melanosporum, both ECM species, also lack PODs and they appear
to have lost many enzymes involved in decomposition of cellulose. Nonetheless, eachhas retained pectinases (GH28) and endoglucanases (GH5), which have been shown tobe among the most highly upregulated genes in ectomycorrhizae (presumably aiding in
the penetration of the root cortex), demonstrating an adaptation of “decay” enzymes for a
symbiotic lifestyle. To further characterize the origins of ECM symbioses, we are
currently analyzing ten new whole genome sequences, including members of theAgaricales, Boletales, and Sebacinales. Preliminary analyses suggest that different
lineages have differentially retained or lost enzymes associated with wood decay,
indicating that there is substantial variation in nutritional capabilities among ECM
Agaricomycetes.

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 15 —-, /=!ånº§Á®s+&®, 5@Â,®, »,®Á v+ .¶â5ã5@Â,®ÁâCochlioborus miyabeanusã»,®ÁâRhizoctonia solaniã
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´¸.%+ *ßÙ]lâ[nqã, ÄzÐc]l, ja]l, È¢ o]lâ©¤´ã, ³Úf]lây|nqã."%m!!\W Í$´¸"Ñª²ÑwÛ4>A?C602;(PMI0009), ©´7D9E¬TÚÅdI )!Y.`$_½¨
1)

Shibuya N and Minami E (2001) Oligosaccharide signaling for defense responses in plant. Physiol. Mol.
Plant Pathol. 59: 223-233.

2)

Kaku H, Nishizawa Y, Ishii-Minami N, Akimoto-Tomiyama C, Dohmae N, Takio K, Minami E, andShibuya N (2006) Plant cells recognize chitin fragments for defense signaling through a plasma
membrane receptor. Proc. Natl. Acad. Sci. USA. 103: 11086-11091.

3)

Miya A, Albert P, Shinya T, Desaki Y, Ichimura K, Shirasu K, Narusaka Y, Kawakami N, Kaku H,
Shibuya N (2007) CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis.
Proc Natl Acad Sci USA. 104:19613-19618.

4)

Fujikawa T, Kuga Y, Yano S, Yoshimi A, Tachiki T, Abe K, and Nishimura M (2009) Dynamics of cellwall components of Magnaporthe grisea during infectious structure development. Mol. Microbiol. 73:
553-570.

5)

Fujikawa T, Sakaguchi A, Nishizawa Y, Kouzai Y, Minami E, Yano S, Koga H, Meshi T, and Nishimura
M (2012) Surface -1,3-glucan facilitates fungal stealth infection by preventing innate immunity in plants.
PLoS Pathogens 8: e1002882.

-1,3-glucan functions as a ‘stealth armor’ in fungal plant pathogens during infection

Marie Nishimura (National Institute of Agrobiological Sciences) 16 —S-2
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1) Orbach MJ, Farrall L, Sweigard JA, Chumley FG, Valent B (2000) A telomeric avirulence gene
determines efficacy for the rice blast resistance gene Pi-ta. Plant Cell 12: 2019-2032.
2) Khang CH, Park S-Y, Lee Y-H, Valent B, Kang S (2008) Genome organization and evolution of the
AVR-Pita avirulence gene family in the Magnaporthe grisea species complex. Mol Plant-Microbe Interact
21: 658-670.
3) Chuma I, Isobe C, Hotta Y, Ibaragi K, Futamata N, Kusaba M, Yoshida K, Terauchi R, Fujita Y,
Nakayashiki H, Valent B, Tosa Y (2011) Multiple translocation of the AVR-Pita effector gene among
chromosomes of the rice blast fungus Magnaporthe oryzae and related species. PloS Pathog 7:e1002147.
4) Freitas-Junior LH, Bottius E, Pirrit LA, Deitsch KW, Scheidig C, et al. (2000) Frequent ectopic
recombination of virulence factor genes in telomeric chromosome clusters of P. falciparum. Nature 407:
1018-1022.

Strategy of plant pathogenic fungi for overcoming resistance genes using a population as aunit of adaptation
Yukio Tosa (Laboratory of Plant Pathology, Graduate School of Agricultural Sciences, Kobe
University) 19 —S-3
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:7 A9HLPTURSQNMPO7 771) Akiyama, K., et al., Nature, 435, 824-827 (2005)
2) Umehara, M., et al., Nature, 455, 195-200 (2008).
3) Cook, C.E., et al., Science, 154, 1189-1190 (1966).
4) Delaux P.M., et al., New Phytologist, 195, 857-871 (2012).

Strigolactones: multifunctional terpene lactones acting as a rhizosphere signal toarbuscular mycorrhizal fungi and root parasitic weeds as well as a shootbranching-inhibiting hormone in plants
Kohki AKIYAMA7 (Grad. Sch. of Life and Environ. Sci., Osaka Pref. Univ.) 21 —S-4«Þa²Ë®ß epichloae 0O=B-/<a²ù*(ñ\~Õ¤ÛåÈ}s
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W£¬+çêltmñ\~Õ¹°èÉ+á(Ö&)( 22 —2) epichloae 4H<?13;]ÂÍÒăK,ûR{í¥ epichloae 4H<?13;«¼ăX~N½Å +W E. festucae Pooideae OÏ Poeae ö«¼%¨, `¤,ć~N«¼4H<?13;P Ñ0î%,«¼ĄÔð«¼Øà(Õ¾äØàØà²~N«¼Ëh ó, vn-,Č4č-*ì *epichloae 4H<?13;~N!¨0ÍÒ,&«¼Ô0b,pü
*~N8;G80KÐ«¼^äÕÂß0b, -,
 epichloae 4H<?13;~N«¼]ÂÍÒêûR{0iĆ,&@
E8B<[yÅ·0Ã~N«¼!±¨ yÅ©0éiĆ-*NADPH ÿhþÖûR{ČNoxAč0°yÅ©~N«¼¨,«¼Ëh(awf0 ó~N«¼( §²Č5čnoxA Ìx©~N«¼Âß0ì.ĀÂ©´"äÕā ĉåwf
*NoxA ¸hq{ NoxR (Va{ā G :H>6ò RacA 0iĆČ6, 7čnoxR Ìx©«¼^Âß0ñ".½ãĈaèÙÛäÕawf
ï&*-E. festucae ),¸ÿÖÂ ~N«¼^äÕa0b, Îo-Č6čusJÂß4H<?13;äÕ0¸ÿÖ¬`îæ¨â,äÕSĂZÓ(äÕaó,ýUrÉ¸ÿÖÂ ¬`-I¡NoxR ùdÈÀ©¸ÿÖrÉÂ z/-äÕZÓĂøäÕaÅ ï&*-$ Nox ą}c_Á)'4H<?13;
äÕa ð-Č6č-*Ú¦¸ÿÖrÉÂ äÕZÓÂĂ(a0b,q{¯á,0Î,SØàZÓ¸ÿÖÂ«¼ªµØàZÓ¸ÿÖÂWĊT +¸ÿÖÕ¾ä«¼ØàZÓÂĂ l®e0,0Îo,* Yeast two hybrid ·0Ã NoxR Úk,q{×0ç.þ³Øà­¶|MÉe0-, Bem1 ( Cdc24 ÊlûR{ iĆ-GFP EAF)+ NoxRRacACdc24BemA Øà^r0ñ".
-*q{ \äÕZÓ(aýr, ¢* Č8č-*Ú¦ *äÕ£ÓÉÂ-,¸ÿÖ äÕZÓÂĂ(a0b, Îo-3) epichloae 4H<?13;¨),~N«¼Ç}mJ
 epichloae 4H<?13;¨)~N«¼Ç} mJ,W vn
-, 4H<?13;¨),«¼Ç}¿D5=9C\í4H<?13;¨ ~N«¼ÇjäÞ0QK,vnW ,I¡\ g¦ ,vn'+4H<?13;ä©(ÇjäÑĊ),g¦ú ,Ý*-,LÄ®st *aĆ- E. festucaeä©Ü#G=2FE37E8ÑÇjÕ¾äuċ0çÚ¦ »Çä Drechslera erythrospila (ºÆÇä Colletotrichum graminicola usJÂß0b, E. festucae ©ČEf437 ©č ë`-Ef437 © ¨«¼ »Çä0Ñ.ć¨«¼´õÇ ô¹,Ym ï&*-ÀrEf437©0Ã »Çä!ä0zyÅ©ÜiĆjqûR{í¥0÷&,
 23 —1) Tanaka A., Takemoto D., Chujo T. and Scott B (2012) Fungal endophytes of grasses. Curr. Opin. Plant
Biol. 15: 462-482.
2) Young C.A., Bryant M.K., Christensen M.J., Tapper B.A., Bryan G.T. and Scott B. (2005) Molecularcloning and genetic analysis of a symbiosis-expressed gene cluster for lolitrem biosynthesis from amutualistic endophyte of ryegrass. Mol. Genet. Genomics 274: 13–29.
3) Saikia S, Takemoto D, Tapper B.A., Lane G.A., Fraser K and Scott B. (2012) Functional analysis of an
indole-diterpene gene cluster for lolitrem B biosynthesis in the grass endosymbiont Epichloë festucae.
FEBS Lett. 586: 2563-2569.
4) Koga H., Christensen M.J. and Bennett R.J. (1993). Incompatibility of some grass-acremonium
endophyte associations. Mycol. Res. 97: 1237-1244.
5) Tanaka A., Christensen M., Takemoto D., Park P. and Scott B. (2006) Reactive oxygen species play a role
in regulating a fungus-perennial ryegrass mutualistic interaction. Plant Cell 18: 1052-1066.
6) Takemoto D., Tanaka A., and Scott B. (2006) A p67Phox-like regulator recruited to control hyphalbranching in a fungal-plant mutualistic symbiosis. Plant Cell 18: 2807-2821.
7) Tanaka A., Takemoto D., Hyon G.S., Park P., and Scott B. (2008) NoxA activation by the small GTPase
RacA is required to maintain a mutualistic symbiotic association between Epichloë festucae and perennial
ryegrass. Mol. Microbiol. 68: 1165-1178.
8) Takemoto D., Kamakura S., Saikia S., Becker Y., Wrenn R., Tanaka A., Sumimoto H. and Scott B. (2011)
Polarity proteins Bem1 and Cdc24 are components of the filamentous fungal NADPH oxidase complex.
Proc. Natl. Acad. Sci. USA. 108: 2861-2866.

Functional analysis of genes involved in symbiotic interaction between epichloae endophyte
and host plant.
Daigo Takemoto (Plant Pathol. Lab., Grad. Sch. Bioagricul. Sci., Nagoya Univ.)

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2ÙĜ` ĭKenneth Bruno ĭSue Karagiosis ĭDavid Culley2ĭShuang Deng2ĭJames Collet2ĭæg¹ 1ĭÂ
»ÿ 1ĭÌĀ· 1ĭScott Baker2ĭàßģd 1 ī1 Ýùç^ÜÖR\DC2 ñOBQ8H>M_C:;CKíçì¯ĬæÔµ#cáã!ĤĈ#"lĭN9<SC! #ÃÖ­{ /Û%#đā#!N9<Ôµ5ÜÝ1°ą Ë+/21¨ÜÖ# ­1ÒÊôó Ö$N9<Ôµ-#µ"Þ/21ý« 1ĪĂ#o1ÒÊôó Ö# ĭþûĚ-þûīKY?YDYLĬ$N9<J
8_EZ#µ}Þ ú /2ĭ2/#ÜÝ«a"çì5ĭėkö*³ °ą
5ÞĪĂĖ+)þûĚ ­"ġ41 4 ëĦ#ęôėk522r|"ĨâÚþûĚ-þû#ÜÝ«5ęôÍ".0Ð÷¿ĭþûĚ ­ęôć n#ĨâÚ" mĂneÕěĝ¤0
þûĚ$ 2.1 sĭþû$ 2.2 s"22ÜÝ« aÈ"Òô´ 18 ia#ğĞþûĚ# ­"ġ41ęôėk5è÷¿ĭþûĚ#ÜÝ«$/"a`¶þû#ÜÝ«$ĜÜÁê¢
þûĚ-þû#ÜÝ«a"£øçì5Ė+1,"ĭ2i#ĪĂ#o1ÒÊôó Ö#ÜÝóÅð,ĊĜ"çì5Ė+1
Research on Increased Production of Hydrocarbons such as Fatty Acids using Aspergillus oryzae.
Koichi Tamano1, Kenneth Bruno2, Sue Karagiosis2ĭDavid Culley2ĭShuang Deng2ĭJames Collet2ĭTomoko Ishii1,
Myco Umemura1, Hideaki Koike1, Scott Baker2, Masayuki Machida1 (1 AIST, 2 PNNL)

O-2 (P-5)
ĩĂ©ĥėk#ċ¾#+#R\V_G_BWHK<QBCIU#Ġâ®ªq  · ĐÏ }À b Ć ī ¡Ģ^tîç ĘùçĬĩĂ#ėkÇý#ċ¾"$ėk#âÚ5 ON/OFF z½1f ©Ĉ2) amyB R\V_
G_ï #ĎR\V_G_#Ġâ ­21 âÚ±~¸",âÚ ĉ/21,#-Ď±~¸"õüxhď# ! ĉ/21ïėkÇýċ¾"pÞ"Ó 1
×"R\V_G_BWHK<Q".1©ĥėk#ċ¾"$âÚ±~¸"Ĕy '(v"± /21©Ĉ 0#. !R\V_G_$Ġâ2!?Z@_CÆ"ċòó".0hď
21ú /21FZPK_Z"åäċ¾5Ą) Aspergillus $FZPK_Z5`# C ÑĒ1 #hďÇÅï"$'6 çì2! FZ
PK_ZĎb#ėkâÚ"S9>\7[9ċ¾5ĄĎ21ėk5²z1,"Çýċ¾5Ą w"#R\V_G_"âÚ~§"pÞýÄČ5Ą
)FZPK_Z)$=X>K_C5ĎÑ?Z@_C5±~¼jS9>\7[9ċ¾=X>K_C"É&FZPK_Z5Þ #¶ ėkvn%#âÚ ! ?Z@_CÛbâÚ Äz2FZPK_ZbâÚ º¬" 10 siaėk 2 ėkĉ R\V_G_#bÎ" EGFP 5ĕ÷RXCTL5Þ¥ēĔ³n5o­#Ø¦¥ēĔ³n5?Z@_C)$FZPK_Zbħău EGFP G]
O>5éč3FZPK_Zb#* EGFPâÚ1 éčÚM_A]ċ¾5Ą w"ć´#Ûb#âÚĎ"ċ¾5Ą1
The development of promoter shutoff system for function analysis of the essential genes in Aspergillus oryzae.
Shiho Terado , Akiko Shimahara2, Rieko Toyoura2, Kazuhiro Iwashita 1,2
ī1 AdSM,Hiroshima Univ 2NRIB Ĭ 25 —O-3 (P-9)
Aspergillus nidulans -D/:>D;H®fÛM'ÒUu¡ÀÈ\Ñ 1â°ZsÓ 1â§ÖmË 1âR¶¥o 2â¸Ã 2âÄ£a 2âÖj 1âR 1 à1 nr
wI®­®hI2 QrIÜIÌá
Aspergillus ~½¿q ¹Øâ-0E,Hâ-0E,Hâ.7Hâ-D/:@H;H(GM) g(' GM â1,2-9:D@H=J5B<8: 1,6 ¾fOØâ1,5--D/:>D=J5[Ø'-D/:>D;H(GF) 1,6 ¾f' Î' GM   GF ®fÛ'³ÆP´·yÍÒUw²¤$&Æ_(gfsA âGF *ÉÊ'W(EB-A2)
$'30;E ¨t &â½¿qN-D/:J5×Ç 60%©| âgfsA
Âº± #`®uÀVL É!%(gfsA ÒUuukÂÙ 7AGH61S
Ù` &âGF Î*½¿qNÂß^ gfsA ÒUu vlGfsA Û'¡ÀÈ*Ï!'!ÁWK gfsA ÒUu 3xFLAG 60*] âin vitro  ' GF f
Õ¼¦«x*Åe»âGfsA-3xFLAGâ¹XMWâgfsA ¯@H=?F9+H*b â
37Yª¢âSDS-PAGE XMâEB-A2 $&È* eÚ"30;
E pbâ¬t¦"#¹XMW*Ýd"30;E É!%(  %â
GfsA-3xFLAG
GF f¦*' %  â2E4W}l' GlfBâ{¿W}l' BipA *@J,J3C¹zcÔÐ`Þ¤$&}lÈ*Å)â
GfsA-3xFLAG 2E4W}l' µ(TK % GfsA
GF fÕ¼&âGF2E4Wf(' µi(Functional analysis of gene involved in galactofuranan biosynthesis in Aspergillus nidulans.
Haruka Motomatsu1, Shintaro Hatakeyama1,Keisuke Ekino1,Taiki Futagami2,Kaoru Takegawa2,Masatoshi
Goto2,Yoshiyuki Nomura1,Takuji Oka1
(1 Univ. of Sojo,2Univ. of Kyusyu)





O-4
Aspergillus nidulans septin interactions and post-translational modifications.
Shunsuke Masuo, Yainitza Hernández-Rodríguez and Michelle Momany (Dept. of Plant Biology, Univ. of Georgia)
Septins, GTPases first observed at the yeast septum between the mother cell and daughter bud, are increasingly
considered to be novel cytoskeletal elements with roles as diverse as those of actin and tubulin. In the filamentous
fungus Aspergillus nidulans there are five septin genes, aspA, aspB, aspC, aspD, and aspE. Here, we investigate theinteractions among the A. nidulans septins using S-tag affinity purification and mass spectrometry. We showed that
AspA, AspB, AspC and AspD strongly interacts each other at isotropic, unicellular and multicellular stages of earlyvegetative growth. AspE appears to have little or no interaction with the other septins in the isotropic and unicellular
stages before septum formation. However after the transition from unicellular to multicellular growth, AspE appears
to interact more with the other septins, especially AspB. LC-MS analysis detected acetylation of lysine residue in
AspA recovered from the unicellular stage and in AspC recovered from the multicellular stage. Western blot analysis
with anti-acetylated lysine antibody showed that AspC is highly acetylated in multicelluar stage. In addition, we
found the phosphorylation of AspD in multicellular stage. These data suggest that the septin interactions andmodifications are altered during their growth stage in A. nidulans.

 26 —O-5 (P-23)
Characterization of Stress Granules in Aspergillus oryzae
Hsiang-Ting HUANG, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The Univ. of Tokyo)

An important part of the cellular responses to stress or environmental stimuli is the modulation of mRNAtranslation and degradation. Recently, evidences from yeasts to mammalian cells have indicated that one aspect of
this process involving the remodeling of translating mRNAs into non-translating mRNPs (mRNA-protein particles)that accumulate in cytoplasmic foci referred to as stress granules. We previously showed that AoSO protein, ahomolog of the Neurospora crassa SO, accumulates at septal pore in response to stresses1). The stress-inducible
behavior makes a possible link between stress granules and AoSO. In the present study, the localization analysis
showed that AoSO-EGFP colocalized with the stress granules visualized by AoPab1-mDsRed in response to heatshock. Deletion of Aoso altered the localization of the stress granules at the hyphal tip. Furthermore, because theability to form stress granules seems to correlate with the survival of cells exposed to stress, growth of the disruptantof Aopub1 gene, encoding one of the major components of stress granules, was being tested under various stressconditions. Finally, the stress granules were often observed in the vicinity of vacuoles at the hyphal tip, and
AoPab1-mDsRed colocalized with EGFP-AoAtg8 in response to heat shock, suggesting that autophagy may
participate in the cellular stress response.
1) Maruyama et al. Biochem Biophys Res Commun 391: 868-873, 2010.

O-6/728>:D93TzÊv· cag1 ÅMlTup1
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ÆTÆX °&"(#Ñlnq XY%w+)ZdÅMl cag1 sÂÉ( RAPD AJ0J,} *Ñ© IX ²OK G13-900B AJ0J ¬~ 15%ÂÉu½À~[o´#58 (cag1-1 trp1-1, 1-6) ,ªÑ  G13-900B AJ0JÁS(BAC DNAÏtrp1+,|Ð$6?1GJH,pVÑ ¶xf cag1 ÅMl,m* *Ñcag1
ÅMlW³Ç Tup1  _9H=1½,5J;(  X §hÆN,m *ÑËk5;H & 234 5;H AAAÏKÐ TAAÏSTOPÐh/728>:
D934<BÑ#Ò Tup1 _ÅMlÏCc.tupA a`Ð ÑmÈE-F9.B
PCR %'ÑÓ Tup1 _ÅMl¹,µÎ´«$lnO ÑCc.tupA  i( ÑT¬® cag1  i W³Ç Tup1p @CÔÈO,uzÑ
Cyc8p # LP(  ¢&)(ÑCag1, Cc.TupA % Cc.Cyc8 Ì LP, Yeast
two-hybird ,Qº Cag1  N Íe)±¿ P(#ÑCc.TupA # LPW³Çg^ÄÑCc.Cyc8  N ÍeÑCag1  N Íet LP,¤ )& Yeast two-hybrid ­ÑCag1
Cc.TupA  LP( TzÊv·ÅMl,R(]¯x,¤c(The cag1 gene required for cap growth of Coprinopsis cinerea encodes a Tup1 homologue.
Hajime Muraguchi, Takahiro Nagoshi, Kazuki Kemuriyama(Dept. of Biotechnology, Akita Prefectural Univ.) 27 —O-7 (P-29)
ņĜíì2 hydrophobin RolA !hğŁĺ$ø_iîØÒĤÌïı¨Ü 1ŉïZºÇ 1ŉŃÄ 1ŉT}m^ 1ŉŅ×° 2,3ŉtÕĩ¶ 4ŉÆïĂª 4ŉļĲ¿µ 1,3Ň1 Ë{ĽQìèíÐw¶ŉ2 ĳđûQöŉ3 Ë{QÇÊûŉ4 Ë{Qoûň
ņĜ$íì2ðŁà´ĝôī hydrophobin RolA %ìuĤ´JM7?EN PBSA #ùŉPBSA uĤĴČ CutL1 #/2 PBSA uĤ5kį2SÁŉÓ"hğŁ! hydrophobin ĺ$ø_iîØÒ.$kinetics %VÃ
23* #ŉ1ŊRolA $ Leu137, Leu142  PBSA '$ď#ĶĠŉ2ŊpH4-10 $PBSA '$ RolA $Ģ ´% pH4 Åŉ3ŊpH4-10 #2 PBSA ¯Ĉ$ARCĿg%Z´ Ĩŉĵ´ 0 #"2ďÍ5­cT$ďÍ!ŉRolA $ pI=5ŋ6 !
ďÍ0ŉRolA $ PBSA '$ù#%ŉYĖ$òÞõø_iîÚ#ŉĨĿě$ŀĿ~óĨ#nĘ´¾3È%ŉÓ"hğŁ'$ RolA $ùÓ§$ĤÌ5÷õ!
ñ"2zõ´ī5aWğŁ'$ RolA $ù5ŉpH7 $Éd QCM #/1ĥjòÞ¦ñ
"2ğŁ#2 RolA $Ģ ´%ŉğŁ$òÞ¦!Ú$øĻ5þÚQĨĿěğŁ#2Ģ ´%40ŉĨĿěğŁ Åďĸ|ã¬ŉhğŁ$ĨĿěÅďĸ#Ĩ#nĘ´þ3RolA %YĢ´
2,ŉhğŁ$Ŀě#/1ùÓ§ñ"2Ę´5¾* RolA % pH4 hğŁ#Ņ Ģ ´5þŉRolA ç¦TÂ#f ³æ"ùĸ$x! rodlet éĶÒĮ©¶ýĦ3ëŉĿěQÑ´5»ğŁ#ŉpH $ñ"2ÉdU $Ģ´ĤÌ5Ğ 2Analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces
Hiroki Tanabe1, Takumi Tanaka1, Keiko Orui1, Kenji Uehara1, Toru Takahashi2,3, Takanari Togashi4, Toshihiko
Arita4, Keietsu Abe1,3 (1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NRIB., 3 NICHe., Tohoku Univ.,

4

IMRAM.,

Tohoku Univ.)




O-8
Trichoderma reesei @NLRAŅìíñÎ#2 bgl2 ñ$«ł±ïß` 1ŉ¡ńĒ 1Àïēĩ 2ÏSÖ 3¥£ĚÖ 4\} 3 ą}â 1Ň1 Ĺ¢¹ÿQìè
2

JST3 ]QìĪä4  DNA ûĄ¸ň

÷õ @NLRAŅìíĊéĜ
2 Trichoderma reesei # @NLRAİeĔ$óë%ħõ
1@NOR?.$ħh-9N<>DRAŇBGLň$Æ2ĉĬĀà´#/@OG8
R?0ì-BHOR?2!#$+ģ32ÈûĄ %ŉXðÔåÎ QM9414 0áì·êę$ T. reesei ñÎċĐÖ5lÆ1ŉÙXb>R:P=R5î ñÎĺ$Ý
ĭ;FKĤÌ5į, 2QM9414 0 6 b$ñ5Ď­03 PC-3-7 Î%@OG8R?/&L-BNIR?#/2@NLRAħìíĘŅ,03ĜÎ2ÈĜ# čėr-9N<>DRA
2 BGLII İeŇbgl2ň# SNP ì 2!Ã0!" ÈûĄ % bgl2
#ì SNP $«ł5ĤÌ2! ŉ@NLRA$ħóë#Ļ2À"úġ5­2!5÷õ!
 2
ďÍ PC-3-7 Î5[!ķì bgl2 pÎŇWTbgl2ňbgl2 üÎŇbgl2ň5Òć@NL
RA$ħìí´#Ļă$ĤÌ5ĞĜhr$ BGL à´% WTbgl2 # ¤"®63PC-3-7 Î/&bgl2 Î$à´()ā¦,SNP #/ BGLII $ØĘ43 2!ĕ03"0@OG8R?#/2@NLRAİe$Ĭs²Ć% PC-3-7 ÎÅ-ŅüÎ!ñ"2¼y5þ!0PC-3-7 #  BGLII %q#ØĘ5%0
@NLRAħìí#Æv"ØĘ5Û 2!þ3ë bgl2 c$ SNP $«ł5½
ľ2,QM9414 Î# Ó$ĤÌ5į, 2
Effect of the SNP in bgl2 in Trichoderma reesei cellulase hyper-producing strain
Yosuke Shida1, Kaori Yamaguchi1, Mikiko Nitta2 Kazuki Mori3, Hideki Hirakawa4, Satoru Kuhara3, Wataru
Ogasawara1 (1Nagaoka Univ. of Tech., 2 JST, 3 Kyushu Univ., 4 Kazusa DNA Inst.)

 28 —O-9]TaZKMþē1O8Ïĕ GH kRptyAº3N endo-"-1,3-YuVfy`§ÕĒzĶĨħ¹Ý Ĵ»Èë½ôĵ
]TaZCûÀÃĠAĉ2Č¾ĖQĝ03%Č¾ĖĢù=C~AĂĊ¬BäĖ,ĝ0:<(N%]TaZBĂĊ¬ÆC~AWbw>" YuVw+L@:<*MûÀAĝNČ¾ĖĢù=ĂĊ¬ĖĥĀAKMĖ1ON%60=]TaZûÀAîé2<(N"YuVfy`BĖÖQď:8%]TaZ²  ÒñB±¶+LC úĲBUwe¨"YuVfy`  D ,þē1O|ĥĀQ\ye3Nģ±QXvygwY28ą×6O7
OÑóBÿĜßĖĥĀ>òÄQÊ8@(0>,ÓL+A@:8%  CÙæBÉĎawiXĜ
='N   >òÄ,ĳ+:8,  CÜċÑóawiXĜ>Cĳ(òÄQ÷1@+:8%zÐ®.BĎĲ@?B[hqĤA²¦3N ! >(@,LJòÄQ÷28%6OLzĈBģ±Ĥ}AC"YuVfy`>Īġ3NrbykCÚ1O@+:8%2+2@,Lþē28   =îé158ăÎ)ĥĀ>JAÓL+A"YuVfy`âÄQ÷28%60=  Q HĥĀĈCÿĜĪġĥĀcyaoy^Ĵ !ĵ
AÏ8@ÿĜßĖĥĀĴ  ĵ>2<ïĩ1O8%|ĥĀCÑóB  kRpty>CòÄ,
.[hqÅ«+LCĥĀÜċCÍµ1O<(@+:80>+LÀJ[hqÅ«9/=@.
ĥĀ³ð@ĖÖ,ĦĔ='N0>,÷£1O8%
Novel endo-"-1,3-glucanase purified from Lentinula edodes
Yuichi Sakamoto Naotake Konno(IBRC)



O-10_usy`ģ±îéÂ¤± ClbR >òì3N¤±BÌā¥ÝĆć 1,2Ķěà 1Ķåěİz 1Ķ¼ 1 Ĵ1 ī¿¯ĬxëêøĶ2 ÒÕ³ĐËčçôü¢ PDĵAspergillus aculeatus =C_usy`xnp_usy`ģ±îéCĶXlnR Q28ÜÛ> XlnR į²
ð@ÜÛB¸@.>JķúĲBĄĞAKMę·1ON%Ç&,µ28 Zn(II)2Cys6 ¨ğ¤± cellobiose
response regulator ClbR CĶ_uvy^ÄªĜQę·æĜ>28ĮB|ģ±ĈBîéQÂ2<(N%0OF=A$clbR õ­ØA*(<JÂ{Bģ±îéC´Aã°2@(0>Ķt\wjfwd ClbR awiXĜ, in vitro =Â{Bģ±mvryaı©AçíðAą2@+:80>+LĶClbR C\SX
boya>2<B¤±>ĚðA_usy`xnp_usy`ģ±îéQĚý2<(N>µ28%
60=Õôü=C ClbR >òì3N¤±BÌāQėG8B=«¡3N%
ÑóBÿĜßĖĥĀģ±Bğ¤±> ClbR isvY+L@N Prey sTlstQē2ĶYeast
Two Hybrid áAKM ClbR >òì3N¤±Q^XtygwY28%6Bą×ĶClbR >òÄ 42%Bi
svY,Á1O8(ClbR2 >3N)%ClbR2 BÜċCÔó='N8IĶF46BÜċQĚEN0>Qñð
>2< clbR2 èõ­ØĶclbR >BĦõ­ØĶD clbR2 ĳîéØQē28%é¦6Bđé¨QĖÖ
2<(NÞĭ='N%F8Ķin vivo A*/N ClbR > ClbR2 BòìA;(<JöĘ2<(N>0P='N%
Screening for interaction factors with transcription factor ClbR involved in cellulase gene expression in
Aspergillus aculeatus
Emi Kunitake1,2, Shuji Tani1, Jun-ichi Sumitani1, Takashi Kawaguchi1(1Grad. Sch. Life & Env. Sci., Osaka Pref. Univ., 2JSPS Research Fellow (PD))

# 29 —O-11 (P-54)
Co-regulation of A. nidulans cellulase genes by transcription factors McmA and ManR
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi¾Graduate Sch. of Bioagric. Sci.,
Nagoya Univ.¿
In A. nidulans, a MADS box protein McmA regulates at least two endoglucanse genes (eglA and eglB) and twocellobiohydrolase genes (cbhA and cbhD). As a MADS box protein generally requires an interacting partner toregulate gene expression, identification of the McmA partner is the key to understanding the regulatory mechanisms
underlying cellulase regulation. One of the candidate cofactors is ManR because it is essential for expression of theabove cellulase genes. This study focuses on the clarification of the cooperative regulatory mechanisms by

McmA and ManR.
RNA sequencing analysis revealed that most cellulase genes were regulated by both ManR and McmA, implyingthat ManR is one of the partners of McmA. Previous studies have proved the existence of two binding sites for
McmA on the 50 bp region of the eglA promoter. To detect the binding of ManR to the region, electrophoretic
mobility shift assay was applied in the presence and absence of McmA. His-tagged McmA and Flag-tagged ManR
were purified and utilized in the experiments. While ManR alone showed very weak binding, McmA alone bound to
the probe with two shift bands corresponding to the single and double occupation of the binding sites. When both
ManR and McmA were applied, the slower migrating DNA-protein complex with enhanced affinity appeared.
Supershift assay using anti-Flag tag and anti-His tag antibodies confirmed that the complex contained both ManR
and McmA. The results illustrated that McmA played a key role in the regulation of cellulase genes by assisting
recruitment of ManR to the promoter.
This work was supported by the Programme for Promotion of Basic and Applied Researches for Innovations in
Bio-oriented Industry.
Co-regulation of A.nidulans cellulase genes by transcription factors McmA and ManR
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ.)

O-12 (P-56)
AtrR  Aspergillus fumigatus +?-/5AD?az$¶Kk&Tv

jg 1ÀOw 2À¨]j 2À}°ns 1Àpµ 2ÀIcYH 1¾1 Zj»´CVzÀ2 [©jC§0B2D¿tmÀ %½§)1D? ªU{RºG$¬| ABC 7=B/:D2D¶Kk&bTv$ Zn2Cys6 f²Pek AtrR &­ $ÁAtrR  Aspergillus oqr Ml %#À]x§ Aspergillus fumigatus !ÀatrR &h$ )1D? ªUº ±y^x& &" $ÁÀA. fumigatus  atrR h $¶Kk9A8(*?&£¤¯$ À
RNA-seq &7=B/,>97D<®&«ÁA. fumigatus ¸h& DMSO, fluconazole,
miconazole `Q .B9?$*B64,/&JW$ ;?39@4,/ RNA-seq&«À`.B9?" 1.91~2.45 M read ·SÀ¡ 10000 ¶Kk9A8(*?&uÁ¹&
³ ¢ ÀhªUW ABC 7=B/:D2D¶Kk  ¬|+?-/5AD?az ¶Kk¹ L ÀªU&W ¼¸%"¹F$E~Àh'iX À$NF   "Á 
" AtrR  ABC 7=B/:D2D¶Kk"À+?-/5AD?az ¶Kk!Tv
$¥"%$ÁA. fumigatus  bHLH f²Pek SrbA +?-/5AD?az ¶Kk&Tv$ "%ÀZn2Cys6 f²Pek AtrR ! %\¯ ¶Kk&Tv $_¦xd %Á
AtrR regulates the expression of ergosterol biosynthesis genes in Aspergillus fumigatus.
Ayumi Ohba1, Kiminori Shimizu2, Daisuke Hagiwara2, Takahiro Shintani1, Susumu Kawamoto2, Katsuya Gomi1(1 Div. Biosci. Biotechnol. Future Bioind., Grad Sch. Agric. Sci., Tohoku Univ., 2 MMRC, Chiba Univ.) 30 —O-13 (P-57)
Neurospora crassa 3I69<KNIÓy¥ĝl*- erg Ė`ąk¡QĦW[ĆVĦģº½ĦOÝ­

Ħú±ÛĤ²ÄĞMÓ~ĥ

Ergosterol èÐöëñó¥jÓy¥ìċď¶Õ¿öl)§Ûöl¹Ù-
Morpholine él Fenpropimorph (FEN)C-14 sterol reductase (ERG-24) C-8 sterol isomerase (ERG-2)YÌĘê/ĝAzole él Fluconazole (FLC) Lanosterol 14-alpha-demethylase (ERG-11)/ĝ¿öÅ£/ØÑ-Ergosterol Óy¥ĝl*, erg Ė` ą.-X Ü+.- ùlĝÌąĖ`ĜeR¬Ì N. crassa  FEN  FLC /iÒF@KLęìċ +Ergosterol ô- 21 ãġ erg Ė`ØÑ¸/ qRT-PCR /ÕÁĎĂ³í´FEN *,ĝÌ erg-24  erg-2 s QÆ erg-25  erg-3 ą.O«FLC ĝÌ erg-11
s QÆ erg-6 ą.ùlĝÌ*,ą.-Ė` ×-X/¬+ õ}ÈTl/ÉyiÒ-FEN ¢æĖ`ą% Ġ÷aQy¥ìċĝøä-×9<KNI*,ą.-Ė`/Â-wò£ ð+.N. crassa Ergosterol Óy¥ìċ (Yãġ×-k¡/u-¨Ëčh/z'čhß µG1BGHN +FEN s FLC ¤u£µ/94HN>L5í´sterol
binding element regulator protein - sah-2 ß µ Ġ÷ FEN ¤u£/áFEN ¢æĖ`ą("#fÇO«sah-2 ß µ FLC ¤u£s FLC *- erg-11 ąěÓµz¸Ą'+._PX +N. crassa čh SAH-2
Morpholine él*,ą.-Ė`/k¡-X/¬+ Regulation of ergosterol biosynthetic genes in response to azole and morpholine fungicides in Neurospora
crassa.
Moto Miyashita, Masayuki Kamei, Masakazu Takahashi, Akihiko Ichiishi and Makoto Fujimura(Fac. of Life Sci., Toyo Univ.)O-14
Chaetomium globosum  -ÍÎÓy¥Ė`Þå +%ÏY¼^ĈÔÎ*-Ė`ØÑk¡ * ®£Ó¾!ĜSUÃ\ĦÝgoOėĦ°¯ĀĦZtÖĦbúĕĦěvqxĦÊĐĊY ĤğÚMùĥ
èÐö Chaetomium globosum ĦmÓÎÅ£/®-¸ÍÎĤY¼^ĈÔÎĥ/Ħõ}ÈÓy¥»·*ÔÓ-¦¯ö -jĖ`Ģ79<E·ç/đĦ*õ}ÈÍÎÓy¥»·Þå/û-¯ØüĦOēÓy¥ÞåĔâ¬+ Ħ¯ö -Y¼^ĈÔÎĖ`ØÑk¡ * ®£Ó¾!ĜS|-Y¼^ĈÔÎ- aureonitol*
chaetoviridin ġÓy¥Ė`ß µ Ħ..×ý©ÍÎĤªÿpyÎ/{&ĥĠ÷ÔÓn ā..+ÍÎĦNMR 9D
4=IĂ³*-·ĒÂ/ûĦîĖ`ß Ģ/đÓy¥Ė`4G9:N/Ï
.+Óy¥Ė`čhØÑĦ..ÍÎÔÓn¢-Ġ÷n_P +Ħaureonitol*
chaetoviridin ġ*Ħ]Y¼^ĈÔÎÓy¥/čhJCIk¡»· ¬+ $Ħchaetoglobosin ġÓy¥Ė`ß µ ĦÀ¥àĄ - Ħ®£ñ ¥.üÑ ā.
.+í´ĦĔrÍÎpÞå +|.-PăÍÎ * .+ġïcÓÎÅ£MdÕ»Ĝ-ÜþĦ * ¯ö -3A82?<0;4k¡ġĖ`ĚßµĂ³í´/Č$ĉć-
Effects of secondary metabolites on transcriptional regulation and sexual development in Chaetomium
globosumTakehito Nakazawa, Kan’ichiro Ishiuchi, Satoru Sugimoto, Yasutaka Gotanda, Michio Sato, Hiroshi Noguchi,
Kenji Watanabe (School of Pharmaceutical Sciences, Univ. of Shizuoka)

 31 —O-15
7L*îđ

l·³tĩĀ¾i,/U[?F7J

àçèğ¤È§ľ¨ŁËÀÆŁªêÛŁĴêėh Ŀå÷©÷`?W;\N7:^Baŀóòÿâđ@MXfj´qcčÁ lÕpġīt / Æ-   Ł%4! Ľ¡Ér!sõâ¶/lÕpġīt!/á æ 'ñä/,£®3/ ´0/0-"Łæ¯ý3¾i/,.ŁsõītĜİ Č!/oŁYH\Ðàîÿâđ/7L*îđMagnaporthe oryzae3éŁæ¯ýĶe/l·³tĩĀ ¾i,.lÕpġèàçèğ¤ Č3ĚŁç·īt3
ÃÚąÌHPtītMoYPD13ø/,.l·³tĩĀ3¾iHPtītø,/ďÖ±3Ĭ/)ŁdÜ{ p38 MAP<KaEOsm1 ītøÎ3xĘ­ŁHPtītø3ĕHPtītøÎ ĻÝfl lÕpġèà çè ğ¤0/3Ěb!Ł7L*îđĥĆ Ðà~}çđĿ9_JQ57Iŀçè 0/U[?F7J ànectriapyrone.Ł*b! ĺĆvÂěàģDNAV7>^6]
7ĝÊ,.Łçèğ¤OFa_bď/ñäOFa_3ùPKSīt3łyĚītø,.Łl pġà çè Ğ)-0  -Ł PKSīt ç·Ķe/Æ- 
È÷ü bĭ!Łç÷D_Fa7MSaAZ_kÑ,/¿¼3
Induced production of polyketide compounds by disturbance of the two-component signal transduction
system in the rice blast fungus
Takayuki Motoyama, Toshiaki Hayashi, Hiroshi Hirota, Hiroyuki Osada(Chem. Biol., RIKEN ASI)




O-16
Aspergillus fumigatus /Âěî

»ĂÔČĝÊ

įnļØŁĐêāŁľÓÏŁmMnĦ ĿĒ`ôđD_Faŀ
6CT\=\Cï!6CT\=\C¦đ,« Ĥ0/ÄĚµÍïŁĎúÐ²ĉ+
AIDS ²ĉ|ìÔČ udPIıíî3« Ĥ gđö-0/A. fumigatus .ŁÙð Ĩ0 ďÖã!ľäŁ ¸çàģ ÙðĔé-0/ ŁĊ±đ ä 0 .ÂěÙðĔ ĵñ ×)-0/0&¢g/PI+àYH\ ŁµÍđ ăċĖĸþ03ĞĢ/]DRFaµÍķ® ıę{ 3/ 0  Ł %4 ¢gz ]DRFa ÷üŁôđz ]DRFa  ,{ 3/Æ- /*!¥È÷ü!Łþĳ3ĞĢ/]DRFaF_O>ģ/]>G_ßÞ3ŁA.
fumigatus ]>G_î± Ķ2.3Ġ$/A. fumigatus @MXĮ,.]>G_°20/īt3Ī¹ŁøÎ3xĘŁV8C# µÍ
Ľ3ĕ1Łî± ĹēÅħÎ Ě  øÎ!î±! îåĄć *ĲçÎë/º3ù .Łøīt î±ñä w{ 3/Ĉ-0/äŁ ītÔČ-ĝÊ3ħ)/1.Łî±Åħ3ö/6CT\=\Cï ñï+½û ÔÒ ĝÆ ħ( Ç¬0/

Screening and functional analysis of the virulence factor in Aspergillus fumigatus.
Kanae Sakai, Motoko Ooarada, Azusa Takahashi, Thoru Gonoi(MMRC, Univ. of Chiba) 32 —O-17.?$Òí(( HsbA º8MB1û Mohsb1ĞMohsb2 ÒrďSs½Nò±ěĉÀ (ÌÈ×).?$Òí ´āÜ ºhÁ8MB1û,ÌÍ ´,¢Þ()!°×ÝĞ·È19Mh÷ÍÈ $Òí-1,3-2K0Mw¢,úĞ°í ´Ē(
,ª&  X~19=O7J1KO;Ã,¤Ěí HsbA 8MB1ûÖÔ$Òí
( HsbA º8MB1ûÒr ďS,¸ø¬kĞ$Òí a¤( 8 b HsbA º8MB1û3O<ă[Ø µ,_¢ę(Òr¸,ïå³Ğ2 Ø µĔîÒr]R ô&)Ğ)&ă[, MoHSB1MoHSB2 {yMoHSB1 !
 MoHSB2 CLHO8ORÄ GFP ,ÿåvÌé6:O5ö,ï*GI>Mq¢ÇYÖ} %`eíà" GFP 42=K ¸g) &MoHSB1MoHSB2 YÖ}hq &·È`e«ÓÊú)( Ù|)mohsb1Ğmohsb2 Ø µõµ¿þYÖ}¢ %`eíà¢ëÐô&)  &MoHSB1ĞMoHSB2  ´}hqĒ è&)hÁ42=K3O<ē,¼ MoHSB1ĞMoHSB2  mohsb1mohsb2
Ø µÒr¼¨,Õñ   &Mohsb1ĞMohsb2 .?$Òí ´āÜ hÁ)ÒrS( Ù|)

HsbA-like proteins Mohsb1 and Mohsb2 are involved in pathogenicity in Magnaporthe oryzae.
Ayumu Sakaguchi, Marie Nishimura.
(NIAS)



O-18 (P-78)
gìć¾ Spindle Position Checkpoint (SPOC)¹¢óâ/JĕÆÑÒíYÖ}hqāÜ(Ăiäêz®ĀïďS(ÅÏuç1)Ğs ½2)ĞUaV1)ĜWąđNÌË1ĝÌÈ×2)ĝ
/JĕÆÑÒí/JÚ·ÈÆÑÒ, ü·ÈÒràÉí(°í ´āÜ hÌêdßĄYÖ}¢Ğ`eíàTf¢ČPÿ¡¢,\Ğ ´,¢Þ(£)!-2LA1:J/Fûý§Â%'Ğ/JĕÆÑÒíYÖ}¢¼¨ÐµcoQ-1,(coQ-1Ø cñă[(¦3O<-E@ĈĆjĞgìć¾BUB2ĘÕx,ÙĞDHL2ă[,CoBUB2{ygìć¾BUB2äêhðlďS(Spindle Position Checkpoint (SPOC)¹¢óâ(CoBUB2ă[Ø ė,ïå³Ğ cobub2Ø
µYÖ}¡ÐĞT·È Òr]R,ÙĞcobub2Ø µ(¶hð¥p,÷²(#ĞYÖ}hqāÜ¶ %ãċ^ö,ïå³ĞĊÌµĖč
4«ĎĞYÖ}¢\ãċ^ gÊ¶hð ï+)(Ğcobub2Ø µĖč2«Ď¯Óìê ¶hð ï+)Ğ2¶(ênw ĔîoĞYÖ}hqāÜ(äêz®,¸ø(#ĞS®Đm %M®Đm,Î¶hð,öå³ĞØ µG1/S®Ûï«® ĞĊÌµ¿þá2«Ď©!( ù#&)ZQ%'Ğ/JĕÆÑÒíYÖ}hqāÜ ĞCoBUB2G1®æ¤Ğ(S®Ăič«®ďS'ĞM®l(gìć¾BUB2Ð(»ë,­(të Ù|)
Spindle Position Checkpoint (SPOC) component in Saccharomyces cerevisiae is involved in proper cell cycle
progression during appressorium development in Colletotrichum orbiculare
Fumi Fukada1), Ayumu Sakaguchi2), Yasuyuki Kubo1)(1)Life and Environmental Sciences, Kyoto Prefectural Univ., 2)National Institute of Agrobiological S 33 —O-19 (P-80)/<5Ē©Ùï 1.0 Mb µíc4P@+* AF ÂåÓy¤Ą_2I8;Pz
v¾è 1ĕāò«dÑ 1ĕ¨±d¬ 1ĕĈÎs® 1ĕß\ 1ĕ±u 2ĕàh}Y 3ĕ¶¹

1

 ē1 {ĊOÓ|Āĕ2 OĀĕ3 ĐOĀĔ/<5Ē©Ùï NAF8 · AF ÂåÓy¤Ą_ēAFTĔ2I8;Pĕ1.0 Mb  conditionally dispensableµíc4P@+*gĕ±µícąn,ÅĕVì¡* 2 4N=.3ēF1
' F2Ĕ (++ 158 eĕ127 eĄ_,zĕAFT Ą_é4N=.3 F2  392kb č2I8;Pĕ č 24 e§ AFT Ą_ 2ė7 4CPl* +( 24 Ą_ 17 eĕ AF ÂåÓÔ *»ë,z[ĕ č4
P@+*ĕAF ÂåÓÔTx¼þjo AftR 'o+*Ą_z,÷$1.0 MbµíciĄ_ĕćÓ· AFTR 6/LN7N3· *þjLDK,J-K;/F RT-PCR
Ç'Ãÿö²ç³ĕAFTR 6/LN7N3·ĕ24 e AFT Ą_"þjLDK Ďñ`Sĕ +(Ą_ AftR '½o+*  ¬( (ĕĄ_Ý'ĕ»ë°ö² 7 Ą_ AF ÂåÓy¤ *»ë,ö²ç³ĕ7
Ą_ 4 e AF ÂåÓÔTx¼)ĕ¿) 3 eÂåÓÔ,¦o*Ą_*  ¬( 
Identification of AF-toxin biosynthetic genes cluster encoded by the 1.0-Mb chromosome in the strawberry
pathotype of Alternaria alternata
Ayumi Hara1, Hikari Kondou1, Yoshiaki Harimoto1, Chiaki Mase1, Yusuke Cho1, Mikihiro Yamamoto2, Kazuya
Akimitsu3, Takashi Tsuge1(1Grad. Sch. Bioagric. Sci., Nagoya Univ., 2Fac. Agr., Okayama Univ., 3Dept. Agr., Kagawa




O-20
â 2 ËĂ9N6Pæý?0HM47#ð´Ùï (Cochliobolus heterostrophus)£µ*]Û¤,o*ÆÈbĕrkċÓ*ĕaÖtY*ĕÌú¯*ĕ¸Öã*ĕÖWs*ēÍûÚOÒáĕ*ZĊĀĔ
H>KÓÏ*kîĆÁ MSB2 & SHO1 (º¤+*â 2 ËĂ9N6Pæý Ü(+
*Ė æýđËĂ8?L8­ MAPKK 1AP: STE11 ,\ MAP 1AP: HOG1 ,É¢q* đËĂ8?L8ă¡,o*ĖQªäÐïďĆÁH>K×)STE11
MAPKK 1AP: HOG1
MAP 1AP:É¢qU  Þ~+*Ė[¥ĕXõ¹ÏÙvï*?0HM47#ð´Ùï (Cochliobolus heterostrophus) ,Õĕâ 2 ËĂ9N6PæýĉU*éĄ_Ý' »ëö²,óĕ¹ÏÙvï*p,ù"ĖSTE11 ' STE50 Ą_Ý·đËĂ8?L8!£w¢Þ ê¤GIBNqfÀ¤' ]Û¤ñTi,ÞĖ üi HOG1
MAP 1AP:Ą_Ý·ø%(+CHK1
MAP 1AP:Ý·ø%(+Ėâ 2 ËĂ9N6PæýXõº¤* MSB2 ' SHO1 Ą_Ý·đËĂ8?L8!£w¢QmÞ  ØÄôČēEJ8<LNĔ *]Û
¤ñTi,ÞĖQªMSB2 ' SHO1 Ý·½GIBNq&ê¤ ø%(+Ė^Rç³ (?0HM47#ð´Ùïâ 2 ËĂ9N6PæýđËĂ8?L8ă¡ £µ*]Û¤,o*RÊ»ë*  Þ~+Ė

The secondary osmotic sensor pathway regulates appressorium formation in Cochliobolus heterostrophus.
*Kosuke Izumitsu, Yuki Kitade, Takuya Sumita, Satoshi Yutani, Atsushi Morita, Chihiro Tanaka(Grad. School of Environmental Science, Shiga Prefecture Univ, * Grad. School of Agriculture, Kyoto Univ.) 34 —P-1
4>9VĦ2òó/ Aspergillus oryzae #tĊā¦õÈ 1ļkĔ 2ļÎĹã 3ļĐ` 2ļĪÁú 2 ĺ1 [ĤįW²òļ2 [ĤWñòĀļ3 ı§WxĀ·įĻùø4>9VĦ$ľi#5SKVĦ2»ËÜĦļr³ķ  -¶Ûč+¾ûw! #|Â¬­uô0 /îļ4>9VĦ$ Aspergillus terreus ",.òó0 /ļÍđ$ Aspergillus ¥ąìđ/ĸđ A. oryzae ! "Ý'òĊğļ«đ$GCÄ Ė´Õđ/ Ĳ/A. oryzae $~Ñ,.ħĞ"uô0 .ļòĊĝ® A. terreus ,.*ĝ !GC&#r³Ù)ķ ü-0 /ÍýĂ$ļ4>9VĦ¶ĥĆ/:;Ľ39EACĦB5SK6:QX=29XD/ CAD1 2 A. oryzae "¢qļ4>9VĦòóČ2Ë
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Molecular breeding of Aspergillus oryzae for itaconic acid production
Satoshi Yamada1, Yuko Kurachi2, Enoch Yongsoo Park3, Motoo Arai2, Shin Kanamasa2(1Grad. Sch. Biosci. Biotech., Univ. of Chubu; 2Dept. Envi. Biol., Univ. of Chubu; 3Grad. Sch. Sci. Technol., Univ.
of Shizuoka)

P-2
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Study on the immobilization of the amidase producing Aspergillus oryzae with cellulosic support.
Akira Suzuki1, Takuma Shitaya1, Takuma Okuda1, Takumi Yanagihara1, Jyun Kimura1, Mtoaki Sano1, Kenji Ozeki1,
Shinichi Ohashi1, Hirokazu Tsuboi2, Takayuki Bogaki2, Kazuya Iwai3, Taiji Fukunaga3 (1KIT, 2Ozeki, 3UCC)

 35 —P-3
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De novo transcriptome assembly of Filamentous Fungi using 454 and Illumina sequencing data
Fumitaka Kawai1, Yasuo Uemura1, Kiyohiko Igarashi2, Chiaki Hori2, Masahiro Samejima2, (1Genaris, Inc., 2Dept. ofBiomaterial and Science, Univ. of Tokyo)

P-4
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Fungal Transcriptome as a Tool to Search for Biomass-Converting Enzymes
Kiyohiko Igarashi1, Chiaki Hori1, Masahiro Samejima1, Yasuo Uemura2, Aya K. Takeda2, Satoshi Kaneko(1Dept. of Biomaterial and Science, Univ. of Tokyo, 2Genaris, Inc., 3National Food Research Institute)) 36 —P-5 (O-2)ĴčµİĦo$ĕÑ$,$S^XaFaBYHK:RBCIW$īó
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Shiho Terado , Akiko Shimahara2, Rieko Toyoura2, Kazuhiro Iwashita 1,2
Ķ1 AdSM,Hiroshima Univ 2NRIB ķ




P-6
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Improvement of the Cre-loxP selection marker recycling system in Aspergillus oryzae.
Silai Zhang, Naoki Ebara, Osamu Mizutani1), Mizuki Tanaka,TakahiroShintani, KatsuyaGomi(Grad.Sch,Agric.Sci.,Tohoku Univ., NRIB1))

 37 —P-7¾đí|¯üãĎ Lecanicillium sp.#ý$Íû

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Construction of transformation system using autonomously replicating vector in Lecanicillium sp.
Kei-ichi Ishido, Hiroshi Kinoshita, Fumio Ihara1, Takuya Nihira(ICBiotech, Osaka Univ., 1Nat. Inst. Fruit Tree Sci.)

P-8
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Analysis of humic acid degradation and reduction by filamentous fungi
Nami Nakazawa, Ken-Ichi Oinuma, Naoki Takaya(Grad. School of Life and Environmental Sciences, Univ. of Tsukuba) 38 —P-9 (O-3)
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Functional analysis of gene involved in galactofuranan biosynthesis in Aspergillus nidulans.
Haruka Motomatsu1, Shintaro Hatakeyama1,Keisuke Ekino1,Taiki Futagami2,Kaoru Takegawa2,Masatoshi
Goto2,Yoshiyuki Nomura1,Takuji Oka1 (1 Univ. of Sojo,2Univ. of Kyusyu)

P-10
Aspergillus nidulans * ugeB ĐnÌõĂÈácģ¨kĨÔĕĉ 1hëÒ 2í¦ú 2°ûÎ} 2ĕÅ¬ 1¤¼h 1ĥ1 ¥^ßÜß^µ²§2 f^ě^ĊĦ
Aspergillus nidulans
9KS`! UDP-7Y:_?(Glc)/UDP-4X6F_?(Gal) 4-1MTX_AĥUGEĦ.:_G*z÷ēÏ Gal10p ğo/RKĔĒ|.Ã*ĐnĥugeA '" ugeBĦ
2 *+( 2  B]L6ć!ħz÷ēÏ Gal10p ++ 58.7Ĥ '" 37.8Ĥ ç¶.ÃugeA ! 1116  371 /RKĔ ()ħugeB ! 1287  428 /RKĔ (*B]
L6ćĥUgeA '" UgeBĦ.:_G )ħi/RKĔZPY 38.8Ĥ ç¶.t½
+(
 UgeA Ě! UGE Ó¶.½ À+*ĥEl-Ganiny AM, 2010Ħ (UgeB ēñÌõ!Á( +UgeB ÌõĂÈĂÈ.
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4X6F<RK4X6B] ¹´ÿ* g·+*'4X6F<RK4X6B]!ħïÝø Î«òô¯¹!´ÿ
ê+
Functional analysis of ugeB gene in Aspergillus nidulans
Masato Tanaka1Ekino Keisuke1Taki Hutagami2Kaoru Takekawa2Masatosi Gotou2Yosiyuki Nomura1   
Oka Takuji1(1Univ of Sojo2Univ of Kyusyu)

 39 —P-11
Aspergillus fumigatus "7S9ELSHY?ĮĀľċĺf"½Čô¢lļŌèŀİŌaÿåŌĆ¥ěŌ¬ĜáuŌŀÐ¨Ō£ºaŊ¤Ō`ŋAspergillus fumigatus #Ã ģ³Ö÷3©Ĭö|Ę0A. fumigatus "BWI9ī!ď0 NĉŁ}$ O-ĉŁ"ŅĻnÍą!#7S9ELSHY?(Galf )ĉŁcy1 01. Galf ĉŁ#ŌčĘŇ,Đĝć!ĩ*.1 0ŉćñë!#ú.1 0ĳ§ A.
fumigatus !
 UDP-Galp . UDP-Galf 3´0 glfA ĺf1ŌglfA Ø!
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ł]0 Galf ĮĀľċĺf! #Æ. !1 .ŌûĄĶ¼"  0Ō
A. fumigatus " Galf ĮĀľċĺf"3Ĵĺfø¶ä!-/Ĩ(t*!Ō;HP²-/ 15
ĂŇ" Galf ĮĀľċmĠĺf3Ĺ·ŌLQY>RW PCR ä3ó mĠĺf[çŌKTC4OW
ē°NY6Y0 ptrA ĺfŌmĠĺf\ç"Âê3»(Ĳ)ªĺfüó DNA Âê
3iġà!Ōiġĺfüó DNA Âê3MVEMS?E-PEG ä3ó ĤØ! o13ĂŇ"ĺfüØ3ÜĈ~­ĺfüØ"<VGYğï3ĥ2ŌņĚ ¦õ#ĩ*.1
.!ŌĺfüØ-/čĕBWI9ī3¸rŌHPLC !-/ N-ĉŁ}$ O-ĉŁ"Üĵ3ĦÔ"ďÕŌ  "ĺfüØĤØ"ĉŁÜĵ"¦õýĩ
1
Exploration of genes that encode a galactofuranosyltransferase in Aspergillus fumigatus
Shintaro Hatakeyama1ŌKeisuke Ekino1ŌTaiki Futagami2ŌKaoru Takekawa2ŌMasatoshi Goto2ŌYoshiyuki Nomura1Ō
Takuji Oka1(1 Univ of SojoŌ2 Univ of Kyushu)




P-12
ĊíĘ Aspergillus nidulans "-1,3-:U6W´ľċĺfõØ"ğïĦÔģ 1ŌăęÙ 2ŌZÏÅî 2ŌŃĽÁ± 1,2 Ŋ1 Ó{XÌÒûŌ2 Ó{ńıXñëòÛx´ŋµ#ŌAspergillus nidulans "čĕÜĈ=:FUfĸĎĭ! ŌMAP 8FYA MpkA Ďĭ3^¯!ĦÔ3Ķ*ÎĘ" MpkA Ďĭ#Ōrėľâ#õ /Ō-1,3-:U6W -$8CW´ľċĺfđ"Įp#v® .Ōrėľâ!#-1,3-:U6W´ľċŊAGSŋĺ
f agsAŌagsB "Įpv®!ìz 03Æ. ! 0ĳ§ŌJEö|°ùĘ,Úëö|Ę!
Ō-1,3-:U6WŊAGŋ³ÖķāĿĢ «w3¹ Æ. ! /ŌĊíĘ! 0
AG "ĿĢ°ĩĪ101."ĔÈ .Ōµ# A. nidulans !
Ă" AGS ĺfõØŊagsA üØŌagsB üØŌagsAagsB aĿüØŌ<WD5=RFU agsB Øŋ3ĵ´ŌAGS ĺf
"ÞĖ3ĦÔ1'!ŌÎĘ! 0 AG "´!#_ agsB ÞĖ 0Ōčĕ^" AG ß¿0 Congo red %"³°[Ä03Æ. ! 0bŌA. nidulans ! 0 AG "«w3-/éðĦ0*ŌAGS ĺfõØ! É 0ğïĦÔ3Ğ"Ō"ďÕ! 0'ŌAGS ĺfõØ! Ō-1,3-:U6FY
A -$8CFYAæ°3Ë0 Lyzing enzymes !0³°3ħk2ŌAG ß¿Ø"čĕ# Lyzing enzymes !-Ç!sĦ10Æ. ! ŀñØ"čĕ-1,3-:U6FY
Aqð!-Ý!sĦ1 .Ōčĕ AG #čĕsĦľċ!0³°!ł]0Ē
.1.!ŌAGS ĺfõØ"Ê¡,×ň Ă"ňÑe! 0ň°í3ã
į"Ō"ďÕ! +ĒŊÎûĄ#ñû@WBY"À¾3ŋThe phenotypic analysis of the-1,3-glucan synthase gene mutants in Aspergillus nidulans
Akira Yoshimi1, Azusa Inaba2, Masahiro Hitosugi2, Keietsu Abe1,2(1NICHe,Tohoku Univ., 2Grad. Sch. Agric. Sci., Tohoku, Univ.) 40 —P-13
ÙÃã Aspergillus nidulans "-8G6InøÚöWÏ±!
red mãÙ%" Congo red pÍ"ý[. Congo

Ôä² 1, oét 2, þ÷¤ 1,2Ć1 ®iĀóJÆÁÇ´e, 2 ®iJª­Îć
# Aspergillus nidulans "Ûá~µ×:8CGWõÜð!  MAP 7CK=6;9KB MpkA
Üð2O!ë¯2ô(/'!Ĉ«ã" MpkA Üð# -1,3-8G6InøÚĆAGSćö
W agsAĈagsB "ñac!Âh .2§, ! .'Ĉ«ã! . 2 Ó" AGSöW"¶â2ë¯.(Ĉ/, 2 öW"Ï±2È æÄxë¯2ô(¨"«V!
ĈagsB Ï± +$ agsAagsB Ï±ĆSùÏ±ć"l!f2{q .
Ûá~"ÆhËë¯"Ý°Ĉ/," AGS öWÏ±"Ûá~ ,#Ĉ-1,3-8G6IĆAGć» -Ĉ«ã" AG n!#P agsB ¶â .§, !  .,!Ĉ/,Ï±# Congo redĆCRć!ą m2Ð)§, ! TuĈÛá~O" AG ·¢±! . CR mL¦"jv2Õ§.(ĈãX!.
CR "pÍ2íZ'Ĉ¼XyĄãX2 CR ¿¼!]/Ĉ_ Ì.ãX! CR 2pÍ
"ĈãX2ākĈL½" CR ¸û ,ãX%" CR pÍû2Öb"Ý°ĈAG ·¢±#ĈãX%" CR pÍû}g 'ĈÛá~µØ!. CR "pÍ2íZ0ĈAG ¹ò-8G6I*7@I# CR !.pÍą §, ! /," ,ĈÛá~ , AG ». CR !êr"ą -8G6I*7@IĂbĈãX%" CR pÍû}.!+ CR mL¦.Ðs/Ć«ÎÕ#ÆÎ<I?K"£¡2mć
The relationship between the congo red-sensitivity and the adsorption of congo red to the hyphae inAspergillus nidulans alpha-1,3-glucan synthase gene disruptants.
Azusa Inaba 1, Akira Yoshimi 2, Keietsu Abe 1,2( 1 Grad. Sch. Agric. Sci., 2 Tohoku, Univ., NICHe.,Tohoku Univ.)P-14
Aspergillus nidulans ! .EHA4I7CK= C !+.Ûá~nøÚöWß"ñac! "ë¯ÀÅRĈÉ§ 1Ĉz`çQĆ®ĀJóÆÑJÆ, 1 ®óJD45ć
Saccharomyces cerevisiae "EHA4I7CK= CĆPKCć. Pkc1p #Ûá~"^Þ!ý1.
:8CGWõÜðĆcell wall integrityĆCWIćÜðć!
OË d2 Aspergillus nidulans !
 CWI Üð"µv"5G>H8\ / .  ,ĈA. nidulans !) CWI ÜðwĈA. nidulans " PKC . PkcA ) CWI Üð¶â.à ,/ .Ĉ PkcA "Ûá~nøÚöWß"ñac%"ýN! ³ì/'!ÎÕYè/ pkcA ¾m±ĆpkcA-ts ±ć!g Ĉ¥!ºhx PkcAĆPkcAĆR429Aćć2 alcA EHFK?KcMÆÇ.±ĆR429A ±ć2YèĈPkcA º¬UMĈPkcA ºh¬UM! .Ûá~nøÚöWß"ñaë¯2åpkcA-ts ±2È ë¯#Ĉăcÿ¾Ć30ćyĄãX2cÿ¾Ć42ć!Ò,!yĄ|nĈúÆx±#-1,3-8G6InøÚöW agsBĈ7@InøÚöWchsDĈcsmAĈcsmB "ñaî/"!ĈpkcA ¾m±#/,"öW"ñaî&3ï,
'R429A ±2È ë¯#Ĉ37ĈPkcAĆR429AćÊÄî¬U 16 ©
üyĄ|nĈagsBĈ-1,3-8G6InøÚöW fksAĈ7@InøÚöW chsBĈchsCĈchsDĈ
csmAĈcsmB "ñaî/,!Ĉ37ĈPkcAĆR429AćÊÄc¬UyĄ R429A ±2PkcAĆR429AćÊÄî¬U!Òĉ©üyĄ|nĈagsBĈchsBĈchsCĈchsDĈcsmAĈcsmB "ñaî//," ,ĈPkcA 8G6In7@In!ýN.Ûá~nøÚöW"ñac!ýN.Ðs/Transcriptional regulation of cell wall related genes by PkcA in Aspergillus nidulans
Takuya Katayama, Akinori Ohta1, and Hiroyuki Horiuchi (Dept. of Biotechnol., Univ. of Tokyo, 1Dept. of Biosci.,Tokyo Univ. of Agric.)

 41 —P-15
38JU8K OS-2 MAP 9HWA+.üĄ¡CUJ:Ğ!w¬{ĝěŀ¢ZŀļËÎŀ`cė_ŀXë¿§ŀċÃè ľÄÔĴVàïĿ 38JU8K!ÖĤ=;HRiħľOSĿýĠ"ŀÖĤ®óÐĈyLR>79BIR° ¯ ĭĐ©z2Æ./' ²"ŀOS-2 MAP 9HWA äßw¬.ĩiā2Ü -ŀ;Q@TWR±øĩiľgcy-3Ŀ*ö¹àİīúĩiľfbp-1Ŀŀ8CPWAĩi
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ÝĈĲ!rĥ¯ ÈĔ.(ŀğ $ãĈľFusarium graminearumĿ!ÒġēÅ2č.
OS-2 MAP kinase regulates various cell wall proteins in N. crassa
Sou Kagaya, Kazuhiro Yamashita, Masakazu Takahashi, Masayuki Kamei, Akihiko Ichiishi, Makoto Fujimura(Grad.Sch.of Life Sci.,Toyo Univ)

P-16
ĽĈ

. MAP 9HWA AoFus3 !çbláCUJ:Ğ!ÌąēÅéĉ Ĝŀ^¢ ÚXŀÖzgür Bayram1ŀOliver Valerius1ŀGerhard H. Braus1ŀ~Â |#ľÄĴVĢàïV®à£ŀ1 <DE4U<UĿæåīÑ Saccharomyces cerevisiae ! MAP 9HWA!# Fus3p "À¯àÏŀ¶L6T
OU®óüĄČ ĳ[.÷ÝĈ! Fus3p MOT;"À¯àÏ ĳ[. { ŀaÍfĚŀã¯òĭĐp 2À.gxŀ²!ìðĽĈ Aspergillus oryzae ! Fus3p MOT;
. AoFus3 Ĉ÷qñĶ ¡ŀ!ĩiíÇ"ķ¶.üĄ ØĈ i¸.z Y½
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1 'ŀAoFus3 ĹZ!üĄs¡!Ē ,ŀAoFus3 çblá. 2 !CUJ:Ğ!¡ ¨ĺ2[ .ą¯ î/ŀ AoFus3 !¡"\CUJ:Ğ!ÀÛ +.¨ĺ2 gY!þÆ+-ŀAoFus3
!çblá.CUJ:Ğ!YÕpą¯ î/ßŀAoFus3
çblá.CUJ:ĞĆk &QUĬ}!lá2ēÅ.) ŀ/,!CUJ:Ğ ØĈª )Ķ ¡. ,ŀØĈª!tàı(regrowth).©z2ēÅ
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1) éĉ,¤± 24 ¥¦»ÂĢć}h hęÙĐ¼ĸ p. 2292

Functional analysis of AoFus3 MAP kinase-interacting proteins in Aspergillus oryzae
Daiki YAHAGI, Jun-ichi MARUYAMA, Özgür BAYRAM1, Oliver VALERIUS1, Gerhard H. BRAUS1, Katsuhiko
KITAMOTO (Dept. of Biotechnol.,The Univ. of Tokyo,1Georg-August-Universit 42 —P-17
Ńē A. oryzae "1æē"©1Ąčuº°íĈ³ņrnņd®ç`ņÑ&ńÓ{ìĳ#ÜÙ#ěÕĸ^ģìù^¼ì±ŅñðA. oryzae ­1IU\]G?fĴńòÞ¢ēĉŅ#āéē" ņēā$ļ".
#Ąč"/21ľÅ1Ąč$ļ£5iĄčĵĨć5Ė ņ#½ġ$1ĄčæēĽ"ľÅ1Ąč"æē mÆ1Ĺ5oÁ$kņA. oryzae " §¡a#@[M_"â5åæē5ĝª1qäĦCVJ=¦Ł5÷ýņWoronin body %21:Y;
NW æē#mÆ5ķ 5Í/ " 1) ņ#Ëã$Ąč#HS_D
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"æēņ{ìĳ)Ě¨ 1Ê!¦ŁĂ5÷ýņ#~¢ÜÙ#ěÍ5Ĝ*Ëã^ĆÖ)ņ§¡a"ìČēā5OYEZ_B_".0ÉņæēĄč"ľÅ1Ąč5Ě¨#ĆÖņľÅ1Ąč#ăŇ Ê!ēāxþ5¶Àņ{ìĳ1
4 EKZE"¼Āļ£"Ĕû1 AoSO G]O=ġ 2)5@_L1Ĭm¢#ö×$ņ{ìĳ5Ėēā# ıì×"á(qb)ņAoSO #¬5Ğ( 3ņēāÉ· /Èúļ£" AoSO |Ŀ1Ú¢ Ě¨2ka#ĆÖ /ņAoSO $æē·#ÐßĻĄčuº"w {ìĳ"Ķ41 Ď½ ċ /2ëņĄčuº {ìĳ"Ķc1 ċ /21j#¢"/!1ěÕ5ĩ+1
1) Maruyama et al. (2005) Biochem Biophys Res Commun Vol. 331: 1081-8.
2) Maruyama et al. (2010) Biochem Biophys Res Commun Vol. 391: 868-73.
Investigation of molecular mechanisms mediating cell repair and regrowth upon hyphal wounding in A.
oryzaeJunpei KAWABATA, Kei SAEKI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo)

P-18
Ńē"

19]LA8K_CEĶĨ Aip G]O=ġ#ÜĎěÕÔ«ĠgņÛėÝĭņĒĵĺ»ņÏ¯ ¥ņÑ& ńÓĸ^ģìù^¼ì±Ņ

9]LA8K_CEĮp"¬1 AoAbp1 5 bait  yeast two-hybrid E=X_M]>".0ę}
2G]O=ġ AipAňD (AoAbp1 interacting protein) #ņAipC
AipD # EGFP ĕG]O=ġ$ē
āxþ"  AoAbp1 z¬ 0ņ9]LA8K_CE" s/ #ÜĎ5Ï1ø2 ņ2)#ěÕ$ aipCņaipD #ö× ,"ħ²Òlb#ìČ- FM4-64#0Ĥ*"î²$ę/2!  1)ņ2/#G]O=ġ#9]LA8K_CE" 1ÜĎ5ěÍ1 5ñð /!1ěÕ5Ė,"ņAipB ",ÜĎěÕ5ĖhÊ"ņĄčďa# MCCńmembrane compartment of Can1pŅ#ÙÀ¢17Y<M]O_R7_F AoCan1 "óñņ9]LA8K_CE#ěÕ5Ė#ĆÖņaipC ö×ņaipD ö×#2
29]LA8K_CE"Īµ ì1Í/!}đįà $î!0ņAoCan1-EGFP$e"ēāĮ#Ąčďa"v¬
/ņł´"Ø½Ąč" ēā#xþĮî!19]LA8K_CE#ÜÙ ¤1ø2aipB $ class I myosin İĲ5@_
L 0ņj#āéē" ,ł´"t¤21aipB ö×$¸/2! +ņthiA Q[T_G_¹ aipB #ïë5Â 3ņ@[M_ĊĮ#¶¿î² .'~ì¢#ïđê#qbĚ¨2)ņEGFP-AipB $ēāxþ-Ąčďa"  AoAbp1 z¬ 0ņAipB
myosinI 9]LA8K_CE"ÜĎ1  A. nidulans #ôę ÇÄ21) Ô«/ņÿ 11 āéē~¢ìè¥@]P6Z]EĘÌĿ
Functional analysis of Aip proteins related to endocytosis in Aspergillus oryzae
Kento MATSUO, Yujiro HIGUCHI, Takashi KIKUMA, Manabu ARIOKA, Katsuhiko KITAMOTO(Dept. of Biotechnol., Univ. of Tokyo)

 43 —P-19
īā;Z=_Aº|ĤĘĚmcreD  MalP 6]G>5F_?A"Ĥd«Ä hĮãe ß©Į¿Đ §¯Įj h  (ÆĦ^ĕ^àÚáÍ¸)¹ĮīāE]O]zČĞ÷àá ĮĎ¦đ-QZF_AøývĖ&ö ğĉ,ĮĖ&1»QZF_AL_R3_B(MalP)Ěm ;Z=_A*,Ĕw[PZº|1-1Á+ -`ÀĮyĀĞÔ.-*ĮC]L:đ[PZ6]
G>5F_?Ar¡æzČ1 -ÑÏ ¡- 1ď#-'ĮGFP-MalP ąC]L:đ1 MalPO\U_C_|´cåÝ/ĮQZF_AĎ¦Ålcf GFP-MalP øýÿ¨ċ¤..;Z=_A1Ù-ė) øýÿ + GFP Ąu×Øý"ðĆ
č'+.ĮÄêò;Z=_A¡c- MalP 6]G>5F_?Ar¡æØý"ðĆìčĮ;Z=_Aº|Ĥ0-Ěm äÉ
- MalP 6]G>5F_?A"±ħČÇ1Ć6]G>5F_?A3:D]ğĥ£*,º|1 -'Į3:D]ğĥ£}Latrunculin B 1â GFP-MalP Øý"ðĆ¥-±ħ1ČÇúÈĮLatrunculin B xÞ
1Ć(;Z=_AÙ³(øýÿb GFP Ąu × Á+ ,ĮGFP-MalPøýÿb +Øý"ðĆ6]G>5F_?Ar¡æÑÏ*-(- í.Ò;Z=_Aº|Ĕw (CreA)VM8D]Ĥd- CreD Ěm ëÉ1pĈĮGFP-MalP 6]G>5F_?A$±ħ1ČÇcreD ëÉ QZF_AĎ¦³Į;Z=_A1Ù( GFP-MalP Øý"ðĆ č'+.  +ĮCreD  MalP 6]G>5F_?Ar¡æĖ&Ĥd-|´ -þµ í.
Involvement of a carbon catabolite repression-related gene, creD, in endocytosis of MalP in Aspergillus
oryzae.
Tetsuya Hiramoto, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div.Biosci.Biotechnol.Future Bioind., Grad.Sch.Agric.Sci., Tohoku Univ.)

P-20
Aspergillus nidulans iîÖ 1ĮË

-L8?Y]ÐC]L:đ PxlA ÑþČÇ® 2ĮĪcïÂ 2ĮÌsě 2ĮMichelle Momany3Į³ăÓ{ 1 Ĭ1 gĦ^ĕĮ2 aĝĩĮ3 @W_@3ĭ
L8?Y]?;HZmęùēÏ¸ Ē)øýģ½é Ñþ-C]L:đ`ñĮèÊàÚ ¬ t¡.-q ĮyĀĞÔ Saccharomyces cerevisiae  1 L8?
Y]ÐC]L:đĬPxl1ĭ ¡ĮÎµ¸ĢĜnÕ¢û¼Ĥd- Á+ .-`ÀĮõÜā Aspergillus nidulans <JS 2 L8?Y]ÐC]L:đ Ċy. ,Į PxlA ĮyĀĞÔ Pxl1 ä-:[_Gzĩ.-ĮPxlA õÜāÛÃ°·°¸)?;HZmęğĉ²~1(¾¢ĮÑþ1ČÁ-1çæ%ĮpxlA Ěm ëÉĬpxlAĭćÝ1ċ¤YG -pxlA =\I_¸Ģė­ĮĠàÉ 86%oc%ĮTF[ *!N43X5Gäª ċ¤.Įzà °¸þ ĨĂocĬ7%ĭÒĮGFP ąC]L:đåÝ*-¨ČÇúÈĮøýđ¨-í.%ĮpxlA C]L:đ¸ĥ£}-K5;\Q5?] B ¥ĠàÉó¶µ1í Įøýà¸ö1ĥ£- Calcofluor whiteĮCongo redĮ *!R7N2]9]¥Ī¶µ1í+ĮpxlA øýÏ¸ô¸zĮGlcNAc ġ ĠàÉ 60%ockbúÈ*,ĮA. nidulans  ĮPxlA zà °¸ *!øýà¸Ĥd-ü¤Functional analysis of paxillin like protein PxlA in Aspergillus nidulans
Taiki Futagami1, Yasuhiro Kajiwara2, Hideharu Takashita2, Toshiro Omori2, Michelle Momany3, Masatoshi Goto1(1Kyushu Univ., 2Sanwa Shurui Co. Ltd., 3Univ. of Georgia)

 44 —P-21
æÑù Aspergillus nidulans \9 AN11104 Äóă½

,v÷ĖÈ Saccharomyces cerevisiae LAS17 4[@´ÌõĪyÖġāĪÖ³£ 1ĪuýfĨ¼Ğ^čÔâ^§Ô, 1 ¼č^L24ĩ
 Aspergillus nidulans  8Y?7B]¬Ėç CsmA N ¹äqU4=]Û¨/¶
,GW2]Ĩmyosin motor-like domain; MMDĩ/°Ð¤ÙA]M8Ċ+ùæsä~"Ġ¬ĕnùæ«¬ėĀ z/¾`²èòu ,|~"Äóx¢ÄÂ ¸ă³Î  CsmA ÛhoÕÄóĜc,ă½ ·$-,i ĖÈD_K2OZCG?8Z_H]9)+CsmA ÛhoÕ,A]M8Ċpþ
 AN11104 /Äóă½/ûAN11104
Saccharomyces cerevisiae  8Y?Z]j3]G<2F_=?¥ĥÄó/¶,A]M8Ċ/;_G, LAS17 4[@\9,$ RACE ă½)+îĆĚÎ~"éÇÎ2]F\]ní :JVE_AQ_?g©-'`ö,/àĈÆ AN11104 ß¿/oÿ.ĘÔ¿ÉċÔñúĒùæsä«×~"¡²×wå Ă-ÔñúĒ *AN11104 ß¿/Õ AN11104 ćèÄóă½ Ģ,ï *-&B1U]bĔlØÒ®xó thiF P\X_A_)+ AN11104 ØÒx¢ó¿/oÿ.ØÒ®x»kb ß¿ĦmüÒ/á$ AN11104 g©- C ¹ä EGFP /ì AN11104-EGFP
ùæsä((¡²Ċôa Ă-ÒCsmA  AN11104 èòuÉċ~"in vivo  ,dðÏÓÙÛhoÕÁąe,Functional analysis of a Saccharomyces cerevisiae LAS17 ortholog in Aspergillus nidulans
Hiroomi Hoshi, Hayami Maeda, Akinori Ohta1, and Hiroyuki Horiuchi (Dept. of Biotechnol., Univ. of Tokyo, 1Dept.
Biosci., Tokyo Univ. of Agric.)

P-22
ħù A. oryzae ,ē¯Ù4_FN0>_ĜĐĔlÄóă½

Ö­ğfĪøěğ¦Ī}º{! Ĩ¼Ğ^čÔâ^§ÔĩÚÙ4_FN0>_ ÜÀÔÏ ,èòuwăÄÂ+èòĊ¬w(4[6IY/ÍòČďwă,4_FN0>_ ģē¯Ù )"ē¯Ù4_FN0>_wĦ-ē¯Ù4_FN0>_ ÐA]M8Ċ(4[6IY/Ð×ÙĈĉÍò#Čď, ĖÈÝ*-, A. oryzae æÑù àĈ-ĖÈ Saccharomyces cerevisiae  Atg11 ē¯ÙĈĉĊ pre-autophagosomal structure (PAS) #ČďÄó, -,ySX\9, AoAtg11 Ĕlß¿/oÿ.ģē¯Ù4_FN0>_Å±¿Ð¤ÙÊeùæ )"wÔ¬ĝ ā*- $ē¯Ù4_FN0>_ÃÙĊ,ï *-,A]M8Ċ(4[6IY/ÃĉĂ/û.AoAtg11 R[47=@_VÍò#Ď%Ĝc, á-ºÞã ĪA. oryzae  , AoAtg11 ē¯Ù4
_FN0>_#Ĝc/)+³* ,/ÚÙĪAoAtg11 ă½(T_5_A]M8Ċµ,Ă/û²Ë^ê¾AoAtg11-EGFP 4_FN0>_T_5_, mDsRed-AoAtg8 /tØÒĂ
/û.GCFÑt/áAoAtg11-EGFP
PAS , ³* 
$ Aoatg11 ß¿R[47=@_VÍò#Čď/ęÙă½,)+ĘÔ¿ëĄÙ¶ª , àĈ-*S. cerevisiae  4_FN0>_)+rsÙÍ
ò#Čď-, Ý*-, Ald6 SX\9 AoAld6 ă½/û.Aoatg11 ß¿ 'Íò#ČďĤ ā*- ê¾)+AoAtg11
AoAld6 ē¯ÙĈĉĜcï *-,ÒYeast two-hybrid Ë/Õ AoAtg11 êA]M8Ċă½/đ&,
Functional analysis of genes related to selective autophagy in Aspergillus oryzae
Takayuki Tadokoro, Takashi Kikuma, Katsuhiko Kitamoto (Dept. of Biotechnol., Univ. of Tokyo)

 45 —P-23 (O-5)
Characterization of Stress Granules in Aspergillus oryzae
Hsiang-Ting HUANG, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The Univ. of Tokyo)
An important part of the cellular responses to stress or environmental stimuli is the modulation of mRNAtranslation and degradation. Recently, evidences from yeasts to mammalian cells have indicated that one aspect of
this process involving the remodeling of translating mRNAs into non-translating mRNPs (mRNA-protein particles)that accumulate in cytoplasmic foci referred to as stress granules. We previously showed that AoSO protein, ahomolog of the Neurospora crassa SO, accumulates at septal pore in response to stresses1). The stress-inducible
behavior makes a possible link between stress granules and AoSO. In the present study, the localization analysis
showed that AoSO-EGFP colocalized with the stress granules visualized by AoPab1-mDsRed in response to heatshock. Deletion of Aoso altered the localization of the stress granules at the hyphal tip. Furthermore, because theability to form stress granules seems to correlate with the survival of cells exposed to stress, growth of the disruptantof Aopub1 gene, encoding one of the major components of stress granules, was being tested under various stressconditions. Finally, the stress granules were often observed in the vicinity of vacuoles at the hyphal tip, and
AoPab1-mDsRed colocalized with EGFP-AoAtg8 in response to heat shock, suggesting that autophagy may
participate in the cellular stress response.
1) Maruyama et al. Biochem Biophys Res Commun 391: 868-873, 2010.



P-24
­¬EwQtz|=Qjf;§°iyS<°Fyo°V[nY°B¦ ®eWO¥7]wG¯b}|­¬EwQH{EwQ EST fg 331-25 =Q #C&%mRNA EwQD& 528  EST  43 J«¨Z&d 331-25
=Q #C&EwQtz|RL% mRNA j(`# % (}|f(
_mHg 3- RACE m 5- RACE m.8+*6/f"$C£PrCr/25).6,>(`#  RT-PCR "$EwQ{EwQHEwQw~F% 331-25 =Q #C&% RNA RL l '-14f"$9q
& 331-25 =Q # RNA EwQtz|RL $EwQ( 2 a¤p?Mª{EwQA  RL # 6 a¤p?MªRL (`# 
#¢cK?Mª"\EwQcK?Mª"\­EwQ
! 331-25 =Q # RNA RL l ' 331-25 =Q # RNA ^
sEwQ RL (`#  adapter-primer (x RT-PCR "$
331-25 =Q # RNA -14f"$9q&_IUk_I_I #C&$k¡ RNA ¡ RNA :© RNA
EwQRL% (`# #Nh@( 'K?Mªp?Mª%v[T3/0hNh 
{uv[X #& 

Identification of conidia specific mRNA in Adpergillus oryzae
Masaru Tsujii, Hiroto Morita, Hiroshi maeda, Yohei Yamagata, Michio Takeuchi
Dept. of Applied Biological Science, Tokyo Univ. of Agriculture and Technology

 46 —P-25
Čĳ

adenosine deaminase-related growth factor ĳiĥhĔ£ċ¨ 1ĸĎéØĉĘÑª 1ĸéðà 1ĸĭ¡õY 1ĸ÷ Ć 2ĸéºÌ 1ĸ[ Ě 1

Ķ1 o¤WăāW¸æüĸ2 L7@ĂòķÈď -, adenosine deaminase-related growth factor (ADGF) !ĸadenosine deaminaseĶADAķÜ¹_·Ħv0n¾ĸADA Ü¹0cþĈÚ(u{0x¶,Åė Ú,
ADGF ĳiĥh!Čĳ¬ , ĸ Øĉ!\É, -$4D6>9þĈ)+kãëíìå, ADGF ĳiĥhĶFv-adaķ0}įĸPichia ħÛìå ÿÃ>VF7
ĝ ADA Ü¹0Ë, 0ôęÍòø!ĸ4D6>9 Fv-ada ĥh ØĉĘÑ0ï¿'ĸd Čĳ ADGF ĳiĥhěÓ ±òøīì RNA §ÝĶRNAiķ)"ĴìåèE2BRXK7>X0èĸ4D6>9 Fv-ada
ĥh ìå½xÔĴìåÔ0ltìå½xÔ!ĸ½x ö­¸ēÕČûk æćġ­ģ° *ĸÍĥhçâ Čûk æćĭ],  É*Aspergillus nidulans )" Neurospora crassa:DO_!ĸ--Ĺp ADGF Öĥh ĥh cDNA 0 RT-PCR )+äµĦv0ôę .ĸ^Ô' 1 p ĥh% ADA Ü¹_·Ħv0snåĸ -* ĥh ØĉĘÑ_,

Characterization of fungal genes coding for a putative adenosine deaminase-related growth factor
Ryohei Nishikawa1, Masayuki Fujita1, Masumi Yoshida1, Syuichi Sekiya1, Satoshi Inatomi2, Goro Taguchi1, Makoto
Shimosaka1(1Division Appl. Biol., Fac. Tex. Sci. Tech., Shinshu Univ., 2Mushroom Lab. Hokuto Co.)

P-26
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polymorphism) MX5X ĢĪĘÑ)+ ĥh* 0 cM
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Search for gene causes a defect in fruiting body differentiation in Pleurotus pulmonarius.
Yu Shimada, Mikinari Ito, Yasuhito Okuda, Teruyuki Matsumoto (Tottori, Univ. of Agri) 47 —P-27
Discovering fundamental mushroom developmental genes.
Arend F. van Peer (IBRC), Yuichi Sakamoto (IBRC).
Mushrooms are important sources of food, medicinal compounds, for industrial applications, waste recycling andenvironmental health. However, cultivation of most mushrooms is difficult or yet impossible. A major obstacle for
improvement of mushroom cultivation technology is the lack of knowledge on molecular genetic mechanisms that
underlie mushroom development. Available information is highly scattered, describing specific, unrelated studies inmodel mushrooms and more recently including individual studies on major cultivated mushrooms.
Despite the high variations in shape, color, composition, substrate and triggers for fruiting induction,
mushroom life cycles follow a general course. Within the developmental stages of the mushroom, especially theformation of hyphal knots and primordia seem highly similar between species. We decided to ‘access’ the generaldevelopmental pathway through interspecies comparison of primordium specific gene expression patterns, including
model as well as major cultivated species. Promising universal, primordium specific genes will be studied in detailin the model organisms Coprinopsis cinerea and Schizophyllum commune by means of gene deletion, fusion to
fluorescent markers and quantitative PCR. Once confirmed to play a role in mushroom development, these genes
will serve as foundation for compilation of a general mushroom development model in two directions; stages
preceding and stages following primordium formation.
At the moment of writing, superSAGE datasets are being assembled and many mushroom species are still
under cultivation. We just started comparison of our first four datasets (dikaryotic mycelium versus primordia) in C.
cinerea and Polyporus brumalis. Considering the effectiveness of other expression-based studies for identificationof stage specific developmental genes in mushrooms, we expect to reveal several universal genes to start our modelin the near future.
Keywords: superSAGE, transcriptome, mushroom, basidiomycete, bioinformatics, developmental genetics.

P-28
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Cdc3 9H>2Â(Cc.Cdc3).5J=* ]7A:HÇ (?<$Vu+ GTP °e9H>2Â@/BFJ) \Z¸Ç­¶Â[¼z¨oTbgÆQtfw+`­¶¥¯®´&­¶·x¶»eÌÄ £(+* Cc.Cdc3 ­¶RÊ'Ì-* .( *%Cc.Cdc3-EGFP .
1638?<D94,­¶­¶ÊÃ³±uhÏ¹ªY©xuh¹7A:H
5 §Î (Cc.Cdc3, Cc.Cdc10, Cc.Cdc11a, Cc.Cdc11b,
Cc.Cdc12) uhÏ¹ªbjWeln8 *d7A:HÆQt.wÉ PCR ')¾,d7A:H l_ eln8Cc.Cdc10  Èi!º SK* [ $7A:Hqyh{O9H>2
Â 7A:H¡MU^~+*µ(+*%Yeast two hybrid . Cc.Cdc3 ¡
MU*9H>2Â.­¶ cDNA (¬,Cc.Cdc10 ' Cc.Cdc12 "#fZ (7A:HPp9H>2ÂATP-dependent DNA helicaseSUMO a E2 Ç«
* Ubc9­¶m.²%* expansinGMF (Glia maturation factor) .*Ç.++ 1 2GJ
HZ (¹ 5 §Î7A:Hfn¡MU.f'¿!,Cc.Cdc3
 Cc.Cdc3Cc.Cdc3  Cc.Cdc10Cc.Cdc3  Cc.Cdc12Cc.Cdc12  Cc.Cdc12 Ë¡MU ½(+

Septin gene expression and interactome analyses in Coprinopsis cinerea.
Tatsuhiro Shioya, Hiroe Nakamura, Noriyoshi Ishii, Naoki Takahashi, Hajime Muraguchi(Grad. Sch. of Bioresource Sci., Akita Prefectural Univ.)

 48 —P-29 (O-7)ĕåÄÃ& hydrophobin RolA jLéďĈÏCMÅ°¬ï¨ÆĀ³ 1ĘÆA¡ 1ĘtĐv 1Ę<aQB 1ĘĔ¯ 2,3ĘX®ø 4Ę ÆÔ 4ĘĊā 1,3Ė1 §_tċ8Ã¿Äª\Ę2 ĂÝÑ8oÍĘ3 §_t8¡¦ÑĘ4 §_t8sUÑėĕåÄÃ&Çď¸çËù hydrophobin RolA Ã[ï35,.16 PBSA eÐĘPBSA [ïăÛ CutL1 #& PBSA [ï)Oÿ&:Ę­jLéď hydrophobin ĈÏCMÅ°¬"
kinetics >&' Ę1ęRolA  Leu137, Leu142
PBSA ÜdąìĘ2ępH4-10 
PBSA  RolA îg pH4 tĘ3ępH4-10 & PBSA Øv/90čJA÷ĘĄ 0 &Ü©)F<Ü©ĘRolA  pI=5Ě6 Ü© $ĘRolA  PBSA eÐĘ@àÉµÌÏCMÅ ±Ę÷čäĎčbÊ ÷T cá z'£pfĘ­jLéď RolA eÐ­ï¨)ÎÌÈ&^xÌù)E?éď RolA eÐ)ĘpH7 ¥G QCM #%ðNÉµÈ
&éď{& RolA îgĘéďÉµ ±Ïĉ)Ó±8÷čäéď{&îg
r($Ę÷čäéďtÜdć `ºĘjLéď÷čä tÜdć{÷Tcá Óh'RolA @îu&!ĘjLéďčä#%eÐ­ È&cá)z  RolA  pH4 jLéďĔîg)ÓĘRolA ½ <I¼eÐćq] rodlet Àąd¬þ Òò'ÁkĘčä8«)éď{ĘpH È&¥G=îgï¨)è&

Analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces
Hiroki Tanabe1, Takumi Tanaka1, Keiko Orui1, Kenji Uehara1, Toru Takahashi2,3, Takanari Togashi4, Toshihiko
Arita4, Keietsu Abe1,3 (1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NRIB., 3 NICHe., Tohoku Univ.,

4

IMRAM.,

Tohoku Univ.)
P-30
ĕå hydrophobin RolA sąrÈL cutinase CutL1 ĈÏCMÅï¨|ēêóĘ¤m Vã 1Ę<a QB 1ĘĔ¯  2Ę3Ę~ · 2Ę4ĘĊā  1Ę2
Ė1 §_tċû8Ã¿Äª\Ę2 §_t8¡¦ÑĘ3 ĂĐÑ8oÍĘ4 §Dûtċû8Ã^ėhydrophobin ÙÀå [&@îu[¶çËùĘÙÀååÙéď}k&A. oryzae)Ã[ï35,.16& polybutylene succinate-co-adipate(PBSA) )i:¾Û»nĒ&Ęhydrophobin RolA  PBSA [ïăÛ& cutinase CutL1 )WÊÁ&PBSA eÐ RolA  CutL1
ÏCMÅ& PBSA  CutL1 )½ÞĘPBSA [ï)Oÿ&RolA j¹ÇďăÛ½Þ°á hydrophobin ù&[1]
'üÑÕĘRolA  CutL1 ÏCMÅĘRolA R His32, Lys34 ĘCutL1 R Glu31, Asp142,
Asp171 ąì%Ę+-7Üdĉ?)íZ[2] ĘRolA-H32SK34S BąrÈLĆÃl RolA´ú CutL1 {&îg tK= Ęîg²£ÑÕ RolA BąrÈL²w& CutL1 {&ÏCMÅāJï)ÎÌÏCMÅāJy'& RolA-N¢ÖýS±čä*42Ą)$ß RolA ;ąrÈL)Më  RolA BąrÈL)×:Ú*47PđñæYÂĘ±čä*42ĄPđ RolA BąrÈL)ôë£pfĘQCM #&
RolA ;ąrÈL #^xPđ RolA BąrÈL CutL1 [vĈÏCMÅï¨öõ&
[1]Takahashi et al., Mol Microbiol. 57: 1780-1798 (2005)[2]¤m$Ę£ûâ^xH 2011  tHìČ p164
Analysis of the interaction between Aspergillus oryzae hydrophobin RolA and cutinase CutL1
Yusei Tsushima, Kimihide Muragaki, Kenji Uehara, toru Takahashi, Youhei Yamagata, Keietsu Abe(1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NICHe., Tohoku Univ., 3 NRIB., 4Tokyo Univ. of Agric. and Technol.,)

 49 —P-31
Ėë Aspergillus oryzae !µçBA8;îl=2;F@3I?GėHypAĘ!ÊË
oÓ
zfv[ęgÃ¦¤ĕęP§àė¦ºHüHühĘ
Hydrophobinė=2;F@3I?GĘ"ßÇëHë!Ü·P Ďcáæð ©ĀÐu-Oõ!Zdć7G>4ú-jć\áæ d».áæðÎ¸đw/_ nèČltj/- *,·Pëß)æHdÊ¸/N -J£Hydrophobin "kÚ!y®!ðď oÓ-ú/«Hydrophobin !6I91G5 *,y®
!Î¸  ~h- {p.-¿ăHĄ¹Hqs!òþ Ì+.-ĖëėAspergillus oryzae Ę + Hydrophobin /6I;-å+.-āXėhypA ěD Ę/jč-!t\xĔ¨ " hypA āX ª(ÏÉ
6I;.- HypA 7G>4ú"QdÊ u-/ö-HypA 7G>4ú!Åúðď$!oÓ ÷°-ÑÐĖë/Qjć\! HypA*#ąĈoÓBA8;/âlµçBA8;îl HypA /ÊËõ¸ *#Î¸!kÚy®
$!oÓµç!VN ³ø/ïenoA AFDI7IeM¾\xĔ *, HypA*#µç HypA /ÊË¾\4FC:5E@1I *,Þòk!¸Á¾/kÚy®L ÂMRÄ- *,oÓ!â±HypA *#µç HypA "Æ Î¸!y®ðďoÓ- ö.

Expression and adsorption of functional peptide fusion hydrophobinėHypAĘof Aspergillus oryzae
Keisuke Domae, Asuka Kase, Harushi Nakajima
Dept. of Agricultural Chemistry, Univ. of Meiji)

P-32
Aspergillus oryzae !=2;F@3I?Gä!µç*#u!÷°âęÕÍiãę¥ê[ìę¸Ć[ĕęP§à ė¦ºHüHühĘ
=2;F@3I?GėhydrophobinĘ"ęÜ·P ëß/YĊ-ßÇëHëē '+.-áæð
7G>4ú-Zdćė70~150a.a.Ę!Oõ7G>4ú,ęë\ d».ęëß)dÊ!ð èČlj/ę ¸!VN)Î¸y®ðď$!oÓ ċN Ô+.-Jé ęĚ!ë²"T Òm!Z=2;F@3I?GāX/ó¢«- ę!^d "%0÷¦.
­ÖÛ"Ėë Aspergillus oryzae ¼Ñę=2;F@3I?G/6I;-.-āX/
4 jčėhypA,B,C,DĘęhypA,B,C "ÏÉ/Øù-.&ęhypA,B,C jÈęSą *#Ką×}²/]òę HypA "QdÊ ęHypB "ëß uęHypC "ý!¯WM"ÏÉć /ö-+ ęhypA āX"dÊ¬ ûbć |gęhypB āX"ëß!YĊ¬ ûb.ędÊ¬ "ûbć À-/ôc-
hypA āX&" hypB āX!¶ *-6F<I! ¸ *#áæðá´þ$!Đ"ęU£!āX *,Òñ. +(ę..ÈÝµç-å +.-ęHypA
 HypB !uÏÉ!ċÿ/¦+-ÑÐęhypB āX/ hypA AFDI7IęhypA āX/
hypB AFDI7I *,ÏÉę!M½ í`7G>4ú eGFP /îlę.. hypA,hypB Są×}² a²/]òí`Ēĉö *,ę=2;F@3I?G!u"AFDI7I¡Ă-/Ùr-â± +.-Characterization and localization of hydrophobins in Aspergillus oryzae
Yui Yamakawa, Chie Ishida, Fuyuka Hayakawa, Yuka Mizuno, Harushi Nakajima(Dept. of Agricultural Chemistry, Univ. of Meiji)

 50 —P-33ãĨþ A. oryzae KBN630 Ähp

,Ę©N=J0@Y?ĖfęïÄäĩĚĪ¢īĜċ ³īĚÓīeď iû 1īéí Ò¡ 1īa č 1ī¿¾ ĕ[ 3īz¾ tÿĩâð®ú>Xĥ

2

ī Ç¾ «®>ī1 ZI4B8ī2 E5RZī3 ĥúîĪëêÅĘöĉµ/Õxĉ!ī` ĨþN=J0@Y?ĩAphĪ),¹¬q q
ąĠÌ &īħÖ xÚÔoß^~Ê , īħÖxÚ)+ Đg],&ī
Aph gáâĨþ/sã¶ăì3FU7YĂē®Ā ĝèÏ&*- ,­!-$Ĩþ:GQª *± aph Ėflā 13 k ĖfïÄ/hpīČĨ , Aph Ô©/× ùÃīaphC ÄīaphA Ä )"aphE Ä Aph Ô©g]īAph Ô©
)+g]Ä/|§,&īaphCīaphA )" aphE Ėf cęX\ęïÄ hĂ/ć%·ÑXùÃpyrG Ėf/OY5YhĂ aphC ĖfuģãL8@YpDelaphC /ã  A.
oryzae KBN630-17K3 Ä/¥Đđ²ī5-FOAX2T<W¼C0MB8/ã  aphC ĖfuģÄ/|§Ë aphA ĖfuģãL8@YpDelaphA /ã  aphC ĖfuģÄ/¥Đđ²ī
aphC aphA ĖfcęuģÄ/|§* aphE ĖfïãL8@YpDisaphE /ã  aphC
aphA ĖfcęuģÄ/¥Đđ²īaphCaphAaphE Ėf \ęïÄ/|§ïÄ AphÔ©/×ùÃīČĨ!aphC Ä»'g]ĩĄÄ ÷ 4%ĪīóĨ!aphCaphA Ä»'g]ĩĄÄ ÷ 24%Ī¾îñ!¶ĒÂÎâ´ò/±Ĕ,ã®ĀĝèbÈ [à¸Multiple disruption of acid phosphatase (Aph) genes in the miso koji mold, A. oryzae KBN630
Shoko YOSHINO-YASUDA, Osamu HASEGAWA, Natsuko ONO, Yoshimi IGA1, Yohei SHIRAISHI1, Yutaka
WAGU1, Tatsuya SUGIMOTO2, Ken-Ichi KUSUMOTO3, Noriyuki KITAMOTO ( Food Res. Center, ACIST, 1
Bio’c CO.,LTD, 2 NAKAMO CO.,LTD, 3 Nat’l Food Res. Inst.)

P-34
Aspergillus aculeatus åÁôy1PGĘ46;AY?NRV9Ėf|§èÞÍ!,ĦīċjÐīÙċĤ[ī}v ĩğ£ĢXáàðĪ
ôy1PGĘ46;AY?ĩFAODĪ!īôy1PGĘ/ĘyêxÎqąīH2O2 /á¬,ėø+īôç°É,ôy@WH8ě

×sã~ü,¤îñ:GQªº*õÝþ Aspergillus aculeatus ! FAOD NRV9Ėf/ 5 ¯rºīNR
V9Ėf fao1~5 /ýþèÞīèÞâÛ
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FAO5 ! N-fructosyl N-Z-lysineīfructosyl valine {¨,º*
!īĉ#ġ+!

)ÞīFAO3  FAO5 
æ©

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Expression of fructosyl amino acid oxidase homolog genes from Aspergillus aculeatus
Haruka Miyatake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi(Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.) 51 —P-35
Aspergillus aculeatus ½¢m­ -glucosidasexµÌäe^, äf¬, ±äõW, zp{ ùòóUº¹ÅúAspergillus aculeatus !, uÎºrh+=RPV>q.º»*
(BGL1), =S2Q9Î*ª¯

+*

, 

_

k

ø, ÎèÇ¯µ!, ¡Ø

-glucosidase1cç*(

-glucosidase ñ*ÂÛ!(+¡ÃÉ!, A. aculeatus

7HM8VE+* 7 Èm­-glucosidase (BGLA, B, C, D, E, I, J Æ) . A. oryzae .

Z¿¸, ÍÚïÐâæÜ£, ªé.ÙBGL1, BGLB, BGLC !ª¯laminaribiose (-1,3) > gentiobiose (-1,6) > cellobiose (-1,4) *æµ¾.Ä(,

BGLA ! cellobiose !#¯.Ä, gentiobiose ømÜ¯.Ä, GH family 3 *ïÐ!OFV5æ. BGL #, p-nitrophenyl--D-glucopyranoside (pNPG) !
mÜ¯.$, îÎd.')áã* BGL *%Ä~+BGL1
GH family 3 n$Ü+$, BGL1 . BGLA +1 :J:1D'îÎdÖ½Ï¶ØÊd¦ê.NCQT6BGL1:J:1D!×÷/LHð'<?A4T6+*
Hð

Êd¦ê

, BGLA !Xßø¦ê+1 :J:1D

Ö½

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yj+&Y*°+gentiobiose .mÜ, cellobiose .mÜvñ!\°+¦ê.íbµ¾À

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*Ü£.ë$*The hydrolytic property of extracellular -glucosidases from Aspergillus aculeatus
Shunsuke Taketani, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi(Grad. Sch. Life & Env, Sci., Osaka Pref. Univ)

P-36
GH3 -6R8;@V>

-IP5DIPH;@V>§¯.

³å[, äf¬, ±äõW, zp{(òóUº¹Å)ÕUÁÀAspergillus aculeatus ½¢-6R8;@V> 1 (BGL1)!, Glycosyl Hydrolase famiry 3 (GH3) , =S2Q9Î.ôìi Ëg (6R8V<ubs«mÜ*ïÐ*GH3 ! BGL _, 4;PT 1,4--4;S;@V>, 6R3T 1,3- -6R8;@V>  ),  W¨¦ê!ö
a*W, -IP5DIPH;@V> (-FFase) !, GH32, 68, 100 , IR5DV<©.á
ã<5SV< s«mÜ.Ý*ïÐ*GH3  GH32, 68, 100 ïÐ!, +%W¨¦êöa!c, GH3 ïÐ -FFase ¯.}%² , ¡ÃÉ , GH3
BGL1 -FFase §¯. Ûl+ , àÑ¥Þ.s ®UÒ¤BGL1 §]Î.Wx, TLC 'Ü£,, <5SV<.¼
|, èÇx»´w"uÎ-+*bÓ<KAD ¥l+#, HPAEC 'Ü£,, º*uÎ!6R8V<* o$, èÇx»´!<5SV<I
R5DV< `s% Ô (+, <5SV<*31GB05<.Ü£,,Km = 11.36 mM, kcat = 0.05 /s ), èÇxt·!, <5SV< 36.2% (mol/mol) ¸, èÇx»´.ÍÚ, NMR '*¦êÜ£.Ù*-Fructofranosidase activity of the GH3 -glucosidase from Aspergillus aculeatus
Takayuki Katayama, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi(Grad. Sch. Life & Env. Sci., Osaka Pref. Univ) 52 —P-37
Aueobasidium pullulans ïÄ- L -1IBACIA7;O9!ıÎ Pichia pastoris
-ŅñèıĈnò©ĨÅîøÖňîPĔ ņĪN¨ëüŇôòöĖ A. pullulans ATCC 20524 Ç +¸ GH family 51 -- L -1IBACIA7;O9Į
Y abfB 04LO@M5Á"ňA. pullulans ATCC 20524 Ç!ÁıĈ0Ĳëì-' ňH:AOKħnıÎ P. pastoris !ñèfÓ0ÈğňıĈnòĤ©Ĩ0¼+
-0ôò¶ÔNċÆOatspelt ïÄ37IM0ãĈÞł A. pullulans ATCC 20524 Ç +b RNA 0­eňcDNA 0x«! cDNA 0ĳ abfB ŀ 0 PCR ňE4:O ¯a¥ P.
pastoris aabfB ĮY!75?KFD<>0y&b ORF (2,097 bp)0fÓ75?Kİg0y$
pPIC3.5 ¯a]ĝÇ0 pABF115 ïÄÇňabfB ĮY!«äıĈŀ 0ıÎ°x~!
fÓ75?K0y& pPIC9 ¯a]ĝÇ0 pABF116 ïÄÇ30 120 ½ĶňØ\®łċÆňA. pullulans ATCC 20524 ÇĖ\ 0.043 U / ml !ıĈ×©0û ňpABF115 ïÄÇ 8.4 U / mlňpABF116 ïÄÇ 4.7 U / ml Ņ ıĈ×©0ûÌ ň*,ıĈ×©
!Ņ pABF115 ïÄÇ!Ø\łQÚ +ň6KâĬ4LG=5IC2O *,ÁıĈ0ļÏÕmò
pP ĂĝĂĝıĈ"fĲĆ 116 kDa  ,ňN-glycosidase dé N ċxĄĴ0ĸs-fĲĆ 70 kDa Û$ň¾ĭ pH " 4.5ň¾ĭÜ " 75 Áùÿ"´įüõæh³ĐùÿĊĦùÿ±īTÉ!Pêę/.
Characterization of Aureobasidium pullulans -L-arabinofuranosidase expressed in Pichia pastoris.
Chihiro Higashida, Kazuyoshi Ohta
(Dept. Biochem. Appl. Biosci., Univ. of Miyazaki)

P-38
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çĖ!ĸs0ę Ģń0·Áñě"ň´nĥ £ W÷4JO@M5ĻąçĖ!ĸ
s QĠ!ıĈ0ĭí-'ňıĈ!t¨ÃX!Èğ0ę!z-"' öĖĉđßĞıĈ0í ň´nĥĀ!Ð²r~Ė!ĉđ!ßĞ0Ģ%!ċÆňë
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Removal of filamentous fungi from oil painting using fungal cell wall lytic enzymes.
Tomoko Wada1, Eriko Nakau2, Noriko Hayakawa1, Yoshinori Sato1, Noriko Ohkawara1, Kiyohiko Igarashi3,
Takayasu Kijima2, Rika Kigawa1(1Nat. Res. Ins. for Cultural Properties, 2Tokyo, Tokyo Univ. of Arts, 3Univ. of Tokyo)

 53 —P-39³ą3G6?9! acetyl xylan esterase (Fv-axe)Èęg!ËāČÂćã¡ 1īċă

1īãêÖ 1ī~ ª 1īï ÿ 2īã®» 1ī^ Đ 1

ĩ1 o[ýû[¬àôī2 O7C!üëĪ
Acetyl xylan esterase (Axe) "6;UZ_!6;Y\=Ì 3=BWù1>@W/{ÏvČ+ěõ*ī³ą ¼ĔõÀ/vČÄħ/Ý©+Ĥ ")þ ),+,% 3G
6?9÷Ā ) Axe é­/îīħąóiÜåç æÞ+ęgĩFv-axeĪ/}ĥ¿ëð"Fv-axe ęgáÛ!Ëā/đÃ
Fv-axe ORFĩ361 1QGĜÌĪ" N ¾ñp(*;8EWĚwī>WY\=ùDR2Zīfamily 1
carbohydrate esterase Ò­DR2Z )+PW@DR2ZÇĖ/º;8EWĚw/ģ
cDNA ·Ú/æÞâM7?\pPICZA ´sīR?G\W­ē|­ěÎ Pichia pastoris /` âæÞīø¶ ?ZI7Ĕ Fv-Axe ¯Ĕ p-nitrophenyl acetate +vČÒ­/î/íďÞ, Ď÷ěõç­Ĕ/đÃ_+¦ëðĠæ RNA ÔĩRNAiĪ (#ĨæÞâH2EV\M7?\/âī3G6?9 Fv-axeęg!æÞ±yÅĨæÞÅ/juæÞ±yÅ!

+Í ĆĘ¤ )īFv-Axe "¼ĔõÀ_!6;UZ!vČxâ ¥ġ.+ ¹) Characterization of acetyl xylan esterase-like gene (Fv-axe) from the basidiomycete Flammulina velutipes
Masayuki Fujita1, Ryohei Nishikawa1, Masumi Yoshida1, Toru Okuhara1, Satoshi Inatomi2, Goro Taguchi1, Makoto
Shimosaka1(1Division Appl. Biol., Fac. Tex. Sci. Tech., Shinshu Univ., 2Mushroom Lab. Hokuto Co.)



P-40
Aspergillus nidulans ! sirtuin È?ZI7Ĕ SirA !ËāČÂfćĄĝ, «Ïra, Æmb, ĨĒèÊ ĩòÑĢ[àßĪ sirtuin "J=CZĂ1>@W|ěõĩHDACĪ7U= vĦ,+ NAD+kç HDAC *àÛä¢n,+sirtuin "J=CZ H4 !Ă1>@W|/lė+ (īLBY7YP@ZÇĖ!§¯!lė¦čęg!<\Z:2XZ;Z8/Ĉ %īsirtuin J=CZd!?ZI
7Ĕ/Ă1>@W|+¨z&&'c!?ZI7ĔĊi/§¯Ëā+& ,+]¸ī5K
+ sirtuin !¨z"$0½Č¹*ī!Éç?ZI7Ĕ'Ċi/§¯
+?ZI7Ĕ"¹) ěÎ! Sir2p 'JC! SIRT 1QGĜĚw Ħh Aspergillus nidulans !?ZI7Ĕ/µövôúÊ/jĉ-īAN10449ĩSirAĪ Sir2p ]!7U=?\/§¯SirA !ø¶ ?Z
I7Ĕ sirtuin Ò­/º (# sirA ęgìÅĩSirAĪ!Ø÷Ā²uÓ!1>@WV<
ZXMW ĞàÅĩWTĪ!,(*&Ĩ  ) SirA
A. nidulans ! sirtuin Ëā+î,%īSirA "BYR1ĕq!à¯ęg7U=?\ (à¯,+=BV8P
C;=@ZNF;VZ G !àáğ {÷Āt NAD+ {Áe^",)!àáğ Õ )īSirA " NAD+×£kç ,)!à¯/±y+þ ),A. nidulans "ī
Sir2p ' SIRT1 Ċi/§¯+?ZI7Ĕ!OSY8 tandem affinity purification 
MALDI-TOF/TOF-MS !°Ð/â SirA ù+?ZI7Ĕ/µö-īElongation factor 2īR
@4FZ¯ěõīÙ;TA7?ZI7ĔĩHsp70, Hsp40Ī ©), )ī,) SirA !Éç?
ZI7Ĕ+ ,%",SirA Ċi/§¯+ā­ þ ),+
Analysis of Aspergillus nidulans sirtuin A
Eriko Ito, Motoyuki Shimizu, Shunnsuke Masuo, Naoki Takaya(Grad. Sch. of Life and Environmental Sci., Univ. of
Tsukuba) 54 —P-41
GO-9BK%'ù)(-1DR1Fąfì~ē¼¾ė\^ú[ėW, Ûº¬ėñ³ÒĕµÃčSÊÜĖ

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%'s¤ )( )&ąf ďGO-9BKù )( + qRT-PCR Â+ÌÙø *,
,ąf 4 kdVÏÈĈw ø"&) &, nap-1 ×¸+Ìö¶ *, GO-9BK%( aod-1 ąfùj© ) , gst $ mig ąf ,ąfù Å~»m, Í(-R1HMT( CCCP #ø"&) &, GFP+Ì NAP-1 ö¶ &, GO-9BKLB90R~», NAP-1 +æïý &¹Ýò (  ®&  )&  &, GO-9BK, J@6RAN-ªl+X, aod-1 +ù(#, Ä§ćå+ÏÊ  NAP-1 +Ä§y, ćy:@P:¦áąf+ù(í&)
Fluazinam induced the alternative oxidase, glutathione S-transferase and oxidoreductase genes in Neurospora
crassaMasakazu Takahashi, Masayuki Kamei, Moto Miyashita, Fumiyasu Fukumori, Makoto Fujimura (Grad.Sch.of Life
Sci.,Toyo Univ)



P-42
Ĕð Aspergillus oryzae ĉHQ?-T;ąfÿpö¶Ć^a 1ėàoĂĐ 1ė|rã 2ėÕ^U 2ė°Áv 2ė¡Ã 1
ĕ1 µĀčS¦Êyė2 µĀčSĀâ5CKÜ_²î¨HQ3MKĖ
Ĕð Aspergillus oryzae 5CKö¶ç·A. fumigatus  A. nidulans b Aspergillus À
HQ?-T;+6TA(ąf ä 4 u A. oryzae 5CKZ!ø"&)(HQ?-T;ą
f (  ®&  &)&HQ?-T;ąfßåÆ%ÿpĈÍ(  Ú ) ±ÖÞĔð A. oryzae ËÊ(ĉHQ?-T;+Z¥ąfÓÑÿpö¶+òA. oryzae ßåcü %s¤ )( û( +ÑÐ(zĈÐ PCR %A. oryzae ĉHQ?-T;ąfÿpĈ ßåÆ$Ē¯ĊĄ%Í(  ®&  %'÷æßåÆĒ¯Ċy%(ĉHQ?-T;ąfÿpĈx+û("½Yc8T4R7T+Ìö¶+òö¶ßåÆ+ØćB@N/K;M=R))Ē¯Ċ+ 48 ¯Ċ 72 ¯Ċ 4 ´e+Ìç·zĈÐ PCR ~»ô«ĉHQ?-T;ąfßåÆĒ¯Ċ%(ÿpĈy õ&)&Ē¯Ċ%(#%'#ßåÆ%(#­ ÿpĈy(ąf   ®&Èö¶+èé'`£ET@I>Hhó$2M:<Tö¶+òA. oryzae ßåcüs¤ā(](Gene transcriptional analysis of metalloproteases in Aspergillus oryzae
Daisuke Sakai1, Michio Takeuchi1, Toshinori Kozaki2, Kazuo Ishii2, Tsutomu Arie2, Youhei Yamagata1(1Dept. of Applied Biol. Chem., Tokyo Univ. of Agri. and Tech.ė2 Tokyo Univ. of Agri. and Tech.) 55 —P-43
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Intron splicing of acid protease genes in Aspergillus oryzae
Ken Ishida, Megumi Kuboshima, Masashi Miyamoto, Hiroto Morita, Hiroshi Maeda, Ayako Okamoto, Youhei
Yamagata, Michio Takeuchi (Dept. of Applied Biological Science, Tokyo Univ. of Agriculture and Technology)

P-44ĻČĝx£ HapX

ĺóí!=GZM62îï/ķč¡-aÏıh , ÓÄ¨ , ¦ñļĬĽ³£ 2, Îď 2, »Ýuf 1, Ďĳ 1ļ1 ¡\ğ, 2 ï
úÁĄ?\ĸ¯Á?, Ľ11õôGM5WM5W:W=[ļDFFĽ! Aspergillus oryzae îï-=GZM62, ĭÛcË DFFîÀþ |óĩÿĥn£ dffA ïì Ę«., îï Į¢.-DFF !ĭā Ċ-+, åĨīĢ ě-^Ç, ĻČ=GZM62!ĭ ĐĂ }ðÍ¸.- 1)HapX ÚĉĔÒ ÷û +, HapX !ĻČ=GZM62îï~¹ĥn£ sreA /Â~- Ê+  ,, SreA /j dffA /įÅô~¹- e¿.- ĠąAspergillus nidulans  -÷û!=GZM62 îï HapX %0İ` .
- 2)Î÷û!, HapX ĺóí DFF # ·Ķ/ę$-, =GZM62îïİ- HapX
Úĉ ĔÊ/õôÇßāÔtaaG2 OZT]A] ~¹_ HapX ĥn£/ģāOV>RI/qđ, Aspergillus
oryzae KBN616 «vĺóíÕĬîÕ *"ĺóíÕ/G[O[/êÿçE1PD:
ID:>ãp×Čĭ/ä\ĵäÐlĹ/ď.. Čp +ľRNA /Ãy, RT-PCR *-æ§, ĺóíÕ!ĬîÕÜ$ dffA ĥn£óíµ´ ĺ - øė.
&a DFF é´/æ§āÔ, ĥn£óíÙĺóíÕ Ç ĺ øė.
.+ āÔ + HapX ! dffA ĥn£ óí~¹İ`HapX ĺóíÕ!=GZM62 îïÌ- ù.
1) vÞ+ÈÎğċ ¥m 2005 ²´¡mĚèēÉĴ p. 286
2) Hortschansky P. et al. EMBO J, 26, 3157 (2007)
Overexpression of the A. oryzae transcription factor HapX significantly increases siderophore production.
Hayato Nakamura1, Takumi Hayashiguchi1, Shouko Yasuda2, Noriyuki Kitamoto2, Motoyuki Shimizu1, Masashi
Kato1 (1Fac. Agric., Meijo Univ., 2Food Res. Center, ACIST) 56 —P-45
ligD Ć\Û*-Ñęñ hapX Û¼x¢úºÒõOć 1ĜÒĚ|ċě 2Ĝu¸oô 2Ĝ¤ÄdY 1Ĝqóđ 1 Ě1 ~ĐMĂ2 ÐÞªë<Mĕª<ě
HapX åÊñ)OĈĊÂĜCKGN=NP CCAAT ĉkê{- Hap ÷{^ØW`ÑĜĀh1n£-Āh -ÎSĎČ* hapX Ć\ÕÌ *! HapX  Hap
÷{^ø¦ ýä.Ĝê»ĜĎ1öĊç&*Ċç)Ďx,ă%æď0-Ć\ÕÌ18L?KNJ-¸ÚáhapX Ć\Û¼µrmÑ(î¨ĜÑñ¼ A.
oryzae KBN616  hapX Ć\Û1ûÓDNA ĒØ}·ãê{w¥ďR- ligD Ć\Û*,ĜØ}é® rË ĔòP²- Ù+.- 1) Z $Ññ¼ KBN616  ligDÛ¼1x¢¢+. ligD Ć\Û¼1ÑĜ ÿĀ®1ôê»Ø}é® rË85%Ęc1ÝhapX Û¼1x¢-  >B5IB5I6I:LÏÐælÕĊç1
8N@-Ć\ dffA ÕÌ Ąì A. nidulans  #2 HapX ¡ē1y - ,2)I3J=4F PCR  hapX Û¼ċÏ¼ÕÌČ1Ãā/Ĝęñ ċÏ¼Ã"dffA Ć\ÕÌČ ]Q- j ÌHapX n£-U§.-[Ć\ÕÌď(úº1ą'-3) Mizutani O. et al. Fungal Genet. Biol., 45, 878 (2008)4) Hortschansky P. et al. EMBO J, 26, 3157 (2007)
Deletion of the hapX gene using a ligD disruptant of an industrial strain of Aspergillus oryzae andanalysis of
the hapX deletion mutant.
Yuichiro Masuda1, Shoko YOSHINO-YASUDA2, Noriyuki Kitamoto2, Motoyuki Shimizu1, Masashi Kato1(1
Faculty of Agr., Meijo Univ., 2Food Res. Center, ACIST.)

P-46
ęñ A. oryzae >LCLjúĊçÏÐďR-Āhèïgúºč¶âĜÒSÍĜ±þĜXsT Ě¹uĐĂMÏÉÐ¾p©ěęñ>LCLjúĊçÏÐ Ĝ>LCLjúĊçĆ\í1×¬n£-Āh  AmyR EJ?N;x,ă%æ1n£- MalR  2 àĀh  ďR-A. nidulans   AmyR Å¦tvýÖ½ßô- .-Ě1ěęñ  MalR
AmyR eôÅ¦t-U§.- ĜÅ¦tÀ¿ ³+ ¸Úá AmyR  MalR Å¦tÀ¿1úº-'Ĝ7J8N;«nn£ ĚCreAěÛ¼ -ÕÌúº *! sGFP-AmyR  sGFP-MalRèïgúº1ô
creA Û¼ -ÕÌ1AN9Lúº*,ý"ê»ĜAmyR n£QĆ\ 7J8N;*(ÕÌ ü.ĜMalR n£QĆ\ ÕÌ ü.  +ĜAmyR
 MalR Ô-Å¦tÀ¿1µ- Ý.ÁĜzàÈçÇ - sGFP-MalR èïgù1ô/ĜðÆ sGFP-MalR ý"fÈçÇė´ ½
 +ĜMalR
üÿaÖ½g_+ bĖ1y-*,Å¦t(­.-ÌĜsGFP-AmyR (zàÈçÇė´ -èïgúº1ą'-¸Úá ÏÚ<L=N4ADN:HLpiÜÖÚá­ąV¾°¯1yô0.
1) Murakoshi et al., Appl Microbiol Biotechnol. 94: 1629-1635 (2012)

Intracellular localization of transcription factors involved in starch degradation in Aspergillus oryzae
Kuta Suzuki, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.)

 57 —

P-47
ñÔ
Úì

( CreA qÓKC3=QoçÌ CreB Â%(5N6S9j

T³ªô¼X¸ôÝô]vnZ ò¦pëáR¹µº«kóñÔ­ÊïiÚçÌÎ+¹º($½·5N6S9%(2<GM.?j+r(Ë¶Ô
(5N6S9j|j{ßgx CreA %j)( À&)
(J>NË¶Ô Aspergillus nidulans CreA KC3=QRÓKC3=Qo%'5N6S9jj)( Äw) '5N6S9y¥aVÓKC3=QoçÌ( CreB %
 CreA ÓKC3=Qo)(Ð&)(ñÔ  creA q creB Â©+d×5N6S9j í+Ú§ñÔ  creA q creB Â©+d×¹Ñ+Y©¯àÍ¨creA Â©¹Ño Ù) creB ¹Ñ íÙ) ®>QEQ5N6S9+°t}z(APSÒ+¯àÍ¨è¹©APS Ø&)creA q creB Â©¡¿APS Ù)#>QEQR5N6S9°t¥a( --IMS;q5N6-IMS;½·+@S7QÚ§%'Ü!Í¨creA q creB Â©
5N6S9y¥aV$)&æb  ½·`UÍ¨ &creA q creB Â©5N6S9j Úì)( Ä)·ycreA q creB Â© --IMS;¹º l¨+¯à(ò¤ÁÈ¹Á:Q<S.@FS8LQkh~Ã¾ÁÈä\«+r Ö*)ó
Glucose derepression by gene disruption for CreA and ubiquitin C-terminal hydrolase CreB in Aspergillus
oryzae Sakurako Ichinose, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind ., Grad. Sch. Agric. Sci., Tohoku Univ.)

P-48
ñÔ

( CreA <QB4ÞéÏÛåÆ¼X¸ôÝô]vnZ ò¦pëáR¹µº«kó(jñÔ­ÊïiÚçÌ+¹º($½·5N6S9%(2<GM.?j+r(Ë¶Ô
(5N6S9j|j{ßgx CreA %j)( À&)
(A. nidulans 5N6S9jê*(_xKC3=QoRÓKC3=QoêW( )(x u )( &5N6S9j CreA KC3=QoRÓKC3=Qo+^j)([)( CreA <QB4ÞOFNm",Ú§ )¤ÁÈ N £É 4xHA <5+Õt CreA +ñÔ½·/09<QÚ§%' CreA <QB4Þ´Ì²(m+Ú§
creA EPJS<Sî| 4xHA-CreA +ãÍ½·6Q9?M4?+ creA Â©fsÇ´Ì²}ð¢( ¶½·é+¯àÍ¨HN?S9+´Ì²t
CreA <QB4Þé ±®DN4?S9+´Ì²}z}ðÔc+5N6S9%HN?S9+´Ì²}zÅ CreA m+Ü!Í¨HN?S9+´Ì²}zÅt CreA <QB4Þâ± Ù)#HN?S9e¾ CreA ±¬¾½·(1@MS;æbEPJS<S+» 4xHA-CreA +½·t$Ù)`UÍ¨ &CreA <QB4Þé ´Ì²e¾ÏÛåÆ j)( Äw)
ò¤ÁÈ¹Á:Q<S.@FS8LQkh~Ã¾ÁÈä\«+r Ö*)ó

The amount of CreA protein is regulated at the post-translational level in Aspergillus oryzae.
Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.) 58 —P-49
Neurospora crassa  beta-1,3-glucan Yt±°Mh fks-1 ®QUr]hu
JK«I, º[, Dzq, ¦{»}e¶BZj¼
S£± beta-1,3-glucan Yt±°Mh FKS1, FKS2 ¢bwO ®Q² Ex ªn Slt2 MAP )6C0XF®Q¬]h Rlm1  Ur $#±¯,
7<¨~ !=4>¤ Neurospora crassa  1  beta-1,3-glucan Yt±°Mh fks-1 i^ y!# fks-1 &Ur#.+6>]hGy# Neurospora crassa  fks-1 &Ur#]h&y!#qRT-PCR  # fks-1 ¨~&§fks-1 a¹¡|LF² spº `¹¤N
Micafungin (beta-1,3-glucan Yt±µmV) #¢bwOR&§²¸¥dW©
!$ !² mak-1 (S£± Slt2 ortholog)c rlm-1 (S£± Rlm1
ortholog)ckPf $! !Neurospora crassa  fks-1 Ur MAK-1 MAP )6C0X®Q¬]h RLM-1 ´H  y!"±
FKS1, FKS2 Ure#  vl $gfp &?;C1C°MhT
fks-1 9@=C1C'3/(&§ %vl9@=C1C·_ 1500bp ³g-5AE
-1500½-300 ·_ w  gfp ² ¸¥o  !fks-1 ³g-5AE-1500½-300 ·_ Y#'*2:C1C1(9®Q¬]hi^ \ $^fks-1 9@=C
1C·_ Y# DNA Y1A8*­¨~&§#

Attempt to identify a transcriptional regulator of fks-1, beta-1,3-glucan synthase gene, in Neurospora crassa.
Masayuki Kamei, Masakazu Takahashi, Akihiko Ichiishi, and Makoto Fujimura(Grad. Sch. of Life Sci. , Toyo Univ.)

P-50
Trichoderma reesei のセルラーゼ生産に関与するシグナル伝達関連タンパク質の解析
日下秀行，古川隆紀, 深谷英嗣, 志田洋介，小笠原 渉（長岡技大・生物）

糸状菌 Trichoderma reesei は，細胞外セルロースやその誘導体に応答してセルラーゼを誘導的に生産する。しかし，誘導の初段階でセルロースは不溶性で直接細胞膜を通過できないことから，どのように細胞外セルロースを認識しているのかは不明である。また，これまでの研究から初発のセルラーゼ誘導生産は構成的に発現しているセルラーゼによって分解された微量のセルロース分解産物が引き金となり引き起
こされるというモデルが提唱されているが，その詳細は明らかにされていない。これまでに当研究室では，セルラーゼ誘導基質の取込みおよび認識に関与するトランスポーターの同定を試みている。網羅的な遺伝
子発現応答解析によりセルラーゼと同調して発現する推定トランスポーター遺伝子を選抜し, それぞれの遺伝子破壊株の表現型を評価した結果，推定トランスポーターMFS031 の破壊に伴い著しいセルラーゼ活性の低下が観察された。本研究では推定トランスポーターMFS031 の生理学的役割の解明を目的として，
この遺伝子の破壊が基質の取込みやセルラーゼ遺伝子発現に与える影響について詳細な解析を行った。その結果, セロオリゴ糖を炭素源として使用した場合，MFS031 破壊株は野生株と同等の基質取り込み能を示したが、セルラーゼ遺伝子の誘導発現能を欠損していることが明らかとなった。この結果より、推定ト
ランスポーターMFS031 はセロオリゴ糖の基質認識に関与している可能性が高いと推定された。現在，酵
母 Saccharomyces cerevisiae を用いて MFS031 の異種宿主発現株の構築を行い，各種炭素源に対する基質取込みについての解析を行っている。
Functional analysis of genes encoding a putative cello-oligosaccharide transporter in the filamentous fungus
Trichoderma reesei.
Hideyuki Kusaka, Takanori Furukawa, Eiji Fukaya, Yousuke Shida, Wataru Ogasawara(Dept. Bioeng., Nagaoka Univ. of Tech)

P-51
 59 —Trichoderma reesei のセルラーゼ生産に関与する MFS トランスポーターの機能解析
古川隆紀, 志田洋介, 小笠原渉（長岡技大・生物）【目的】糸状菌 Trichoderma reesei は, 細胞外のセルロースやその分解産物の存在に応答して誘導的にセルラーゼを生産する。古くから T. reesei はセルラーゼ生産のモデル生物として様々な研究の対象となってきており, そのセルラーゼ生産制御機構に関しても無数の報告がなされている。しかし, 細胞外セルロースの認識機構やセルラーゼ誘導物質の取り込み系に関してはほとんど知見が得られておらず, セルラーゼ生産制御機構の解明における大きなブラックボックスとなっている。本研究では, セルラーゼ生産に関与する糖トランスポーターの同定を目的として, 遺伝子発現プロファイリングに基づく推定トランスポーター
遺伝子の選抜と遺伝子破壊による糖トランスポーターの機能解析を行った。
【方法および結果】セルラーゼ生産および非生産条件下での網羅的な遺伝子発現応答解析を行い, セルラ
ーゼ生産に関与すると推定される推定糖トランスポーター遺伝子の選抜を行った。選抜遺伝子についてその遺伝子破壊株の構築を行い, 結晶性セルロース培養におけるセルラーゼ生産性を指標に表現型の解析を行った。その結果, ある推定トランスポーター遺伝子の破壊に伴い, 大幅なセルラーゼ生産の遅延と菌体生育の遅れが観察された。また、セロビオースおよびラクトース培養においても同様にセルラーゼ生産性と生育能の低下が観察された。現在、遺伝子破壊株を用いて様々な糖類に対する取り込み挙動の解析を行っている。
Identification and functional analysis of a MFS transporter gene involving cellulase production in thefilamentous fungus Trichoderma reesei.
Takanori Furukawa, Yosuke shida, Wataru Ogasawara (Dept. of Bioeng., Nagaoka Univ. of Tech.)P-52
µ^Y°º£' Aspergillus aculeatus clbR ÆuÂqtOÈe¥¨È´VÈ´ÃJÈq`\a (¾sj¿H)« Aspergillus aculeatus  3EFI2$'¹Qk²o*[w'dk cellobiose
response regulator (ClbR) È3EFI2x XlnR ÁUl²o(' cellobiohydrolase I (cbhI),
carboxymethyl cellulase 2 (cmc2), FIa-xylanase (xynIa) ¹Qk_ XlnR $&²o(' carboxymethyl
cellulase 1 (cmc1), FIb-xylanase (xynIb) ¹Qk*P¸' {  ClbR *©±R¼dk¹Qk (tef1) ?FBI5I[wL zÆ*T­È3EFI2¡;,-A2Y°º£M'uÂ*°
ClbR Æ0G8FIE*pÇ *£gÅÀ Xylanase, Endoglucanasey*m )ÈXylanase y  ClbR ÆgÅZº£» ¢ 3 Wi^ &ÈgÅv"Æy*¦|J}ÈEndoglucanase y È0G8F
IEº£»Ä¬r®%("gÅvySL§# 
(%y¶d'bº£zY*³'!gÅ 4 ~ 8 ~gÅKNY5G</µ*@?69A2=+G.I?DG8*cm¤ÈClbR Æ ClbR [wL'º£ XynIa Y» ]i^  %ÈXynIa
Xylanase yKuÂ
'ª%( ÈEndoglucanase yuÂ'ª%('º£Y»Ä¬r¯n(fÈbº£»b·X»½*³'!ÈClbR [wL'¹Qk»*:I1
G>F78*m»'
The effect of Aspergillus aculeatus clbR overexpression on cellulosic biomass-degrading-enzyme-production
Ayano Kawamura, Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi(Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.)

P-53
h pH $'

«3ECI4[w 60 —]ÒàÚà×SWàµàs ÞnÙ®rËM®«£¡ß³² 3KF:NED<AL4°~±* Aspergillus nidulans A517 !àCMC .o&zx
®¿'8JHN9®¯ ¢*@KN #/Å++$ ¶»8JH
N9®¯~± gvÑY!à3KF:NEPw* ¹t+* à m Á ¶»!àgvÑY palC *.m +.i pH 64>IL4
8JHN9®¯ ØÎ.Æ |p*¤M½ l() A517  *gvÑYkºDN2NÑY IL5N7.
Ç½àpalC ÑYqÌD<B+palC *! Í^w* xlnR gvÑY*jÀ.Ç ½xlnR _! A517 ~±.´ÃÕ® *
xlnR ·}'TÄ1L=4J2>N9 EglAEglB ®¯`Û.Q   palC _() A517 8JHN9®¯ uPalC ! pH 64>IL4ØQ*vÊav PacC
P¥Z¾*PacC DNA ½lÓc! EglAàEglB .o&ºÝ 8JHN9h" ®¯ØQ*Êav ManR BKFN;NÜywCMC .fOª¼¨*ÔR¦[zx
8JHN9É. Zymography ()Æ,àÔX!Õ® palC ~± * EglAà
EglB É Ð! OÕ®!RXÔX()Ö EglAàEglB .®¯* palC ~±!©§¬wpacC ·}.È% )+'\|p*U *¶»!®¶8L;N 0?CN6GLeb{¸²¶»ÏV
O­Â-+' *Regulation of fungal cellulase synthesis by ambient pH.
Kentaro Miyamoto, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ. )P-54 (O-11)
Co-regulation of A. nidulans cellulase genes by transcription factors McmA and ManR
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo KobayashiÞGraduate Sch. of Bioagric. Sci.,
Nagoya Univ.ß
In A. nidulans, a MADS box protein McmA regulates at least two endoglucanse genes (eglA and eglB) and twocellobiohydrolase genes (cbhA and cbhD). As a MADS box protein generally requires an interacting partner toregulate gene expression, identification of the McmA partner is the key to understanding the regulatory mechanisms
underlying cellulase regulation. One of the candidate cofactors is ManR because it is essential for expression of theabove cellulase genes. This study focuses on the clarification of the cooperative regulatory mechanisms by

McmA and ManR.
RNA sequencing analysis revealed that most cellulase genes were regulated by both ManR and McmA, implyingthat ManR is one of the partners of McmA. Previous studies have proved the existence of two binding sites for
McmA on the 50 bp region of the eglA promoter. To detect the binding of ManR to the region, electrophoretic
mobility shift assay was applied in the presence and absence of McmA. His-tagged McmA and Flag-tagged ManR
were purified and utilized in the experiments. While ManR alone showed very weak binding, McmA alone bound to
the probe with two shift bands corresponding to the single and double occupation of the binding sites. When both
ManR and McmA were applied, the slower migrating DNA-protein complex with enhanced affinity appeared.
Supershift assay using anti-Flag tag and anti-His tag antibodies confirmed that the complex contained both ManR
and McmA. The results illustrated that McmA played a key role in the regulation of cellulase genes by assisting
recruitment of ManR to the promoter.
This work was supported by the Programme for Promotion of Basic and Applied Researches for Innovations in
Bio-oriented Industry.
Co-regulation of A.nidulans cellulase genes by transcription factors McmA and ManR
Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ.)

P-55
Aspergillus nidulans *DL>>N9®¯d£¡ 61 —ÖďgdğĘ£¿Âğēbhğ¾Á çğÅ ĝĖÞČ[ÞÚÌËĞ

æå A. oryzae  .Ċs ManR !ğQZJJ\A[@WU\AÞß¯Ě -]¹ğA. nidulans ! manR Ďk ê QZJJ\AÞß° ªę0aA.
nidulans ! manR OSY9- manS 0½(ğÀéð! A. nidulans  - manR  manSQZJJ\AÞßx­# a0Ć$¹Ð[öÆ manRğmanSğmanR/manS ^ï êÇ -6ZIQZJJ\AÞß°0đÞÇÎċkonjac glucomannan (KG)0Ĉ-MX\H3E@5!ğmanR Ç 6ZIQZ
JJ\AÞß° / l`ğmanS Ç!¥l`manR/manS Ç!Þß° qÒCMCğKGğ" locust bean gum áÂ galactomannan (GM)0]Øõ×Ómě
ě_Õ0ğKG 0Ĉ-=5S9UL4\*,ĄÄđÞÇ! CMC  2 ïğGM  2 ï 6
ZIQZJJ\AÑ° Èu.ğKG !.+0/ 4 ï&ğCMC ą 2 ï!
manR êÒğGM ą 2 ï! manS êÒCMC ą 2 ï! GM 0Ĉğ
=5S9UL4\_ î|§!cĂ6ZI9W7J\A EglAğEglB q]ûj_ + ManRnå  CMC ą 2 ï! EglAğEglB ,ğKG 9W:\??HXED0v·-ì.«ğôñ± 6ZIQZJJ\A! ManS *,x­.-ù+.-
Àéð!ğÞé@ZC\ 5KN\>TZzuëåéðµčeÉ ]ÝĀ/.) -Regulation of mannanase synthesis in Aspergillus nidulans
Ayako Watanabe, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi(Graduate Sch. of Bioagric. Sci., Nagoya Univ. )P-56 (O-12)
AtrR ! Aspergillus fumigatus 6W;?FY\W³òĎkäÛ0x­-Í ğÕÏr® ğýí ğ¸ć¡¨ 1ğ¤Àč 2ğf}d 1
122

ĝ1 Ã~ĖČ[ÞÚßÉz³ğ2 þ[çü@ZC\Ğ©éð!ğ.&Ĝü 3B\Wòÿy´uĔa-ā¶ ABC HUZ?P\C\Ďk äÛ0¼x­- Zn2Cys6  Ċs AtrR 0ă-ĠAtrR ! Aspergillus ¢¥¦ o. ,ğã°çü Aspergillus fumigatus  )ğatrR 0ê-3B\WòÿyĔĉ²°0ì0»+ -ĠÀéð!ğA. fumigatus
atrR êÇ -ĎkäÛMYL25W0÷øåĆ$-(ğRNA-seq 0àHUZ?8VMH\RĄÄ0ĀĠA. fumigatus đÞÇêÇ0 DMSO, fluconazole,
miconazole tÜ<ZMWâ-5ZGE8?0i{-QWDMXE8? RNA-seq
0Āğ<ZMW + 1.91~2.45 M read Đwğó 10000 Ďk äÛMYL25W0¬ĠäÛĒ0ÎċöÆğêÇ!ÿyÙÔ{  ABC HUZ?P\C\Ďk ā¶ 6W;?
FY\W³òĎk äÛĒ Ê(l ğÿy0Ô{ė!đÞÇ.+ äÛĒ _º]¹ğêÇ!%1|ğ-!p _º ì »+ Ġ 
 + AtrR ! ABC HUZ?P\C\Ďk '+ğ6W;?FY\W³òĎk äÛ)x­-ù+.-ĠA. fumigatus ! bHLH Ċs SrbA 6W;?FY\W³òĎk äÛ0x­- è+. ğZn2Cys6 Ċs AtrR ).ĆĎkäÛ0x­ú° ì.Ġ
AtrR regulates the expression of ergosterol biosynthesis genes in Aspergillus fumigatus.
Ayumi Ohba1, Kiminori Shimizu2, Daisuke Hagiwara2, Takahiro Shintani1, Susumu Kawamoto2, Katsuya Gomi1(1 Div. Biosci. Biotechnol. Future Bioind., Grad Sch. Agric. Sci., Tohoku Univ., 2 MMRC, Chiba Univ.)

P-57 (O-13)
Neurospora crassa

6W;?FY\WÞ³ĕy*- erg Ďk 62 —ąx­ZęadĀ`ęĖ´·ęXÙ¥ęöª×ė«¿ĐVÎĘ
Ergosterol ãËòæíïtÎ{çĄć°Ð¸òw( ×òw³Õ,
Morpholine äw Fenpropimorph (FEN)C-14 sterol reductase (ERG-24) C-8 sterol isomerase (ERG-2)cÇċå/ďAzole äw Fluconazole (FLC) Lanosterol 14-alpha-demethylase (ERG-11)/ď¸òÀ/ÔÌ,Ergosterol Î{ďw)+ erg ĉi ÿ-,b Ø*-, õwďÇÿĉiĎl[¤Ç N. crassa  FEN  FLC /rÍLETUČçĄ *Ergosterol ð, 21 ÝĔ erg ĉiÔÌ²/ qRT-PCR /ÐºĆü¬è­FEN )+ďÇ erg-24  erg-2 x ZÁ erg-25  erg-3 ÿ-X£FLC ďÇ erg-11
x ZÁ erg-6 ÿ-õwďÇ)+ÿ-,ĉi Ò,b/¤* ñ~Ã]w/Ä{rÍ,FEN âĉiÿ% ēójZ{çĄďôÞ,Ò;?TWR)+ÿ-,ĉi/»,zî ë *-N. crassa Ergosterol Î{çĄ'cÝĔÒ,v/y,¡Åąq/|,&ąqÛ ®P1HPQW *FEN x FLC y®/;6QWCU7è­sterol
binding element regulator protein , sah-2 Û ® ēó FEN y/ÜFEN âĉiÿ'"#oÂX£sah-2 Û ® FLC yx FLC ), erg-11 ÿčÎ®|²þ&*-hYb *N. crassa ąq SAH-2
Morpholine äw)+ÿ-,ĉi/v,b/¤* Regulation of ergosterol biosynthetic genes in response to azole and morpholine fungicides in Neurospora
crassa.
Moto Miyashita, Masayuki Kamei, Masakazu Takahashi, Akihiko Ichiishi and Makoto Fujimura(Fac. of Life Sci., Toyo Univ.)

P-58
Histone deacetylase HstD  LaeA :3D?0>61U=P69OU¼pĂ` 1, 2, Æđe 1, 2, Zø 1, 2ė1 ĐVnàÚ,2 ĊéÚĘ

ì¦ãËòÝ²c¶gāÊ (SM) /ÎÏ,È, SM ÎÏĉi(vµ± ¾Ö-, SM ÎÏĉi6P;=Wv,F;AUmĕĉi Ď\, ĈÜ-) ì¦ *, Aspergillus oryzae /Ð Histone deacetylase (HDAC)ĉiÛ ®/k, 82:ČVIC9QU/M@R SM ÎÏĉi6P;=WÔÌ^  SM ÎÏ!Ē/ü¬è­ċ¹ Hst4 JMT7, HstD
,SM ÎÏvvĎ\,/¢ûús$, ĈêÑá)+ãËò_ù SM ÎÏv, LaeA , 82:ČÎÏ/·v, }-, 82:ČVIC9QUÎÏv/M@R, c¶gāv , HstD  LaeA
:3D?0>61U=P69OUü¬/÷£½Vè­HstD  LaeA :3D?0>61U=P69OU¨É/¤* ,&HstDLaeA®/kü¬è­HstD E>67PUB,'Ď.*LaeA ®|²82:Čx IC9QUÎÏ/Ü HstD  LaeA v/f SM ÎÏ/v,b Ü-HstD  LaeA 4G;=?0>6Ďl*¯ý,&LaeA ĖÔÌ^  HstD E>67PUB LaeA ĖÔÌ/÷è­HstD E>67P2UB'
OE::LaeA ®|²82:Čx IC9QUÎÏ/Ü HstD  LaeA YÁkÐ, ¤* -$LaeA S5NSW=WĎ,} *©Úß
LaeA vĎ,§u},
Analysis of genetic interaction between HstD and LaeA
Moriyuki Kawauchi1, 2, Masato Hirose1, 2, Kazuhiro Iwashita1, 2(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2. NRIB)

P-59
K5ă

Óò

,AQ8?<UÎ{vĉi Tri6 tÎÊÕü¬
 63 —X `TĨmÑ TùĨ ğ]Ĩ² }Ĩ­¯ Ý (yěÎ{Č)J6Ĉ !Õ÷ Fusarium graminearum 9J6ĖûãĢ³Ĉ !Õ. ĉ  säãç.Z¹]ăÏÉAM9@<Qé½êH29A5:Q¿³+®÷AM
9@<QÎwęW+Ē_î7L;>S.*,)Ē_îØÌ7L;>S
fĒ_ Tri6 (k,+ÌAM9@<QÎwìĊ.¶+Ē_îēh x
,$¸òü±&ď/+&k+ TRI6 Ăćę$Vč©ēh 
F. graminearum Ē_ë%¥¡ÎÉćñĒ_.đ¢HS3SjÐ+ Â TôÙ+ ĖĒ_ë%¥.ùĝđ¢HS3SâĢ(+kĚ.u +Ç'ÚÙ+Ĕi^ đ¢HS3SØÌ3<?A °Ē_Ĕi.ë¥a³õaU»$
~ġ \ Tri6 .ü±+*đ¢HS3SĔi.³õaU»ë¥µt.Ú£F. graminearum  HSV-tk Ē_.ÐD4@1F<O7:KQé.ĐÐ®D4@1F<O7:KQé.Ð+¦ Tri6 % e,Ôµ.bg+n
AM9@<QÎw"Ġ.X TRI6 ØÌÊÁ'ïýĀcģ«Èā´vâü±.ù+
Molecular analysis of Tri6, trichothecene biosynthesis regulatory gene in Fusarium graminearum.
Yuichi Nakajima, Kazuyuki Maeda, Hinayo Ichikawa, Tetsuo Kobayashi, Makoto Kimura.
(Nagoya Univ.)

P-60
èÊ÷ Coleophoma empetri

F-11899 µ"[0:LS=Ē_eXĄ |YĨÑ Ğ[Ĩf qYĨàą ÃĨ·® ºÀ ĦÜRÎÉħÅÝ÷ÖÀ×ø micafunginĦ0;@L;úøħèÊ÷ Coleophoma empetri F-11899 µÎÏ+FR901379 0:NdĘ.§í÷Ò°0:LS=.Ðó0:NpĨªĐp,0:NdĘ.«¸w(*pwÎÏ,+úĎáĨC. empetri F-11899 µ§í÷ØĕĤĨó0:Na.r¨¼Ĝáù-,+¼Ĝá.æÓRoËp+ÍĆö'9;AlÆ¬+Ĩ,$®Þåó0:Na.Û¤ÎÏ

empetri F-11899 µt.ÚÙĨ§í÷

,+ C.

)0:LS=Ē_.7PSCQ8, C. empetri F-11899µe, ó0:NaÛ¤ÎÏµt.þ%)Ĩó0:NaÎÏßÿrĨ§í÷Ò°0:LS=Ē_Ĕi GC zė
9BQđ¢èÊ÷,)¾#ĥ





Ĩ$

§í÷3EÔ+ð),Ĩ§í÷Ò°0:LS=Ē_Ĕi.

C. empetri F-11899 µ9BQªĐp[0:LS=Ē_.b C. empetri F-11899 µe[0:LS=Ē_.ØÌGL;IBe ATMTĦ Agrobacterium tumefaciens medieted
transformation ħÂ(*ćċ¥ÌĨó0:NaÛ¤ÎÏµ;7MSCQ80:LS=
ØÌRÄā´+Gene transfer of artificial acylase gene into fungus Coleophoma empetri F-11899.
Kazuya Nakaya, Masato Yamada, Takuya Ouchi, Yasuhiro Isogai, Seiji Hashimoto(Dept. of Biotechnol., Toyama Pref. Univ.)

P-61
 64 —öÚćdÑhĘàØß´ĥøùāt¢ēÇj¿«ĽâbÝ¤Ľ¹ę¡©Ľe~cĻÆĮ]ĝ]ßØàÌz´ļğ¦ĽöÚć&¸ÿćßà*ïdÑhĘàØß´Ĭ,*ġi8W?B^ Ñ
+Ľb%ÙÁá%-Ĺßà'}Õç*ıĺć Aspergillus
oryzae åïdÑhĘàØġi8W?B^-r*éèÀúàØ-ßà *%
+*_ºĽ|ÛèdÑhĘàØß´°-Ā*ĽöÚć'êÊßØ ß´ ùāĚ  n( 4Y6HWtč,+*Ă±-Ā³*¯ď *g¬ĽĺćåïdÑhĘ÷-rĽĹßà*$ 4Y6HW¢w­ ħď*Ā(+*Äëð ĽVDYËØæć Phoma betae ßà* DNA polymerase ĭy*0M1
D1<X\ß´ġi8W?B^¢-ēÇ*-éèß´ĥø GFP -ČB\J8Ě-ĺćçÜĽċqĵ®ĩĒ-čĽČB\J8Ě¢-Ē"ĽP. betae '*0M1D1<X\ß´üě ®ßØ (}ËØă*ßØä¨ u¥*VI47>;G^A* Cytochrome P450
2 ïĶ* ĽÀúÓİĬ,* P450-1  N Ãòo >9GYPNCFÎĤv * (Ľ+(į '*Č
B\J8Ě¢%Ē-čþĽ4Y6HWÙåè*4Y6HW
R^5^B\J8Ěô RFP -ČB\J8Ě-ĺćtsçÜĽß´ĥø¢ģl
1) Fujii et al. Biosci. Biotechnol. Biochem (2011)

Subcellular localization of biosynthetic enzymes for secondary metabolite in the filamentous fungus.
Akihiko Ban, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
( Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ. )P-62
öÚć Talaromyces stipitatus ãÅ MT FU2\-Á*B2N I  PKS ÐĂēÇÏp ė 1ĽÈí 1ĽĆĈ² 2Ľâ§¨ 2ĽĊf  1

Ļ1 £µî]ĉ, 2 ñĎë]ßĉļ
?CLBC\Ħßàć Talaromyces stipitatus  Ľ?CLBC\Ħxĸm* 3-methylorcinaldehydeĻMOļßàQX:B2F´ĥøĻPKSļ-<^F* Ts0002pks ġi{ĽTs0002PKS ÎUCYEW\?M3W^AĻMTļFU2\-%ĴĢp NR-PKS -<^F* 3 ġi * ;ITēÇ')¾( *ĽTs0021pks
-0@CY MO -´*NR-PKS -<^F*ġi*- 1)gĽÒ+ÐĂÂìĕ 2  MT 
Á NR-PKS ġiĽTs0058pks ĽAspergillus oryzae åïçÜ'*ÐĂēÇ-Ĕ#*Gateway Ô') Ts0058pks ġi- amyB N[V^B^`û#Ğ.çÜNW?SF pTA-Ts0058pks-ÍõĽA. oryzae M-2-3 ªĚĜ·ĽrªĚĜ·m- 3 ¼īĖķ¬ĽķÖ- HPLC uÇýÉĽpTA-Ts0058pks ªĚĜ·m <\E[^Y Đ(+Øßà ìĕ+ĳĽÍĠēÇ-čýÉĽEXKF[7>GMBZ\Þ0@CYUCY-%¹đ
O7=:B2FĦm* ìĕ+Ts0058PKS ĽTs0002PKS & Ts0021PKS Î
FU2\Í´-% NR-PKS ) (Ľ×øĨĪ (kĪß´Ø-a* PKS Ā(
+Ľ+( PKS  *×øĨĪ '!UCYrw­ÐÍĄ ¶+*1) ÏpėĽĊfĽó 10 öÚćußØ<\M/Z\?ď½ĲĽp.34
Functional analysis of type I polyketide synthase genes containing MT domain from Talaromyces stipitatus
Makoto Hashimoto1, Daisuke Kobayashi1, Daigo Wakana2, Yukihiro Goda2, Isao Fujii1(1School of Pharmacy, Iwate Med. Univ., 2Natl. Inst. Health Sci.)

P-63
Penicillium purpurogenum '* Monascus ąø»mßàĂÎ±ēÇ
 65 —ïmÀĝ³¬ìĝ¿ĝĎZĖĝ£ĐĝkñĘĝ¨öĔ¡ ě¦PÊÅþÁPÊslĜĝ¯-8-ÑċÊÅjÌ) ð Monascus Seð PenicilliumÊË(fĄYº\üËÅ÷°(Seð Penicillium ©T\ºß§&)( ĝPenicillium ð Monascus ĒÊ»T\\üËÅÊoĉ^½^ēąS§(ĝ«ØÝ Penicillium purpurogenum ( Monascus íäp¤`ÊËê~µ+÷° ĝPenicillium purpurogenum # Monascus íäp¤`Êoēąĉ^%ĉ^ËÅ´å+ó&ĝ)&ĉ^ PenicilliumMonascus Ē(½^nê+´ø(" Penicillium purpurogenum ([Ċyĉ^´å+ó8 Üę
Penicillium purpurogenum +ÌíäÊËê+Ùùè±ĝíäÊËčć (# 6 ð
²  12-carboxyl-monascorubramineĝ12-carboxyl-monascorubrin ÊË+ÙùíäÊË#$ĝÆÒÞä\üê+ 2 ð²+ÙùØÝ ÷° Penicillium purpurogenum
5BH@KD?7Q3/O9+Ì Aspergillus, Penicillium àáÇð &ög)(¥×
polyketide synthases |zĊi{+zĝ«áÇð polyketide synthases ĉ^´å+ local BLAST
search %'´åè±ĝã 20 b polyketide synthases ĉ^x+Ùù&ĝpµ«áÇð5BH@KD?7Q3/O9+Ì¥×áÇðÎ®[Ċyĉ^|zĊi{+zĝ[Ċyĉ^´å+óè±ĝMAT 1-2 ĉ^ÕpĚĉ^x+Ùù

Analysis of the diversity of Monascus pigment homologues productivity by Penicillium purpurogenum
Jun Ogihara, Sara Umemura, Ryo Kojima, Kasumi Koganei, Teppei Arai, Jun Kato and Takafumi Kasumi (Dept. of
Chem. Life Science, Nihon Univ. )P-64
Aspergillus fumigatus 5BH÷û² Af293 vĉ^p(DG?MJL8OėÊËmkñÔ¶ 1ĝďªt 1ĝ¬î 2ĝĚ·aY 1ĝđÍôW 1 ě1 ÉØzØP4G1LC.0N8Qĝ2ĚăcÚ

ØÝ:O;QĜDG?MJL8OÐmáÇð Aspergillus fumigatus ÊË(F.6?27O(5BH÷û¸'Êoĉ^ěftmĜ3K9;Q p)  &5BH÷û² Af293²  ftm 3K9;QS¾l({r)"ÊoçĀeý÷§ wĕDG?MJL8OÊË²( BM939 ²+Ì(ÊoçĀ÷°+ó 
«ØÝAf293 ²{r)(DG?MJL8OÊË¹ĉ^Òõvp+ÓÒftm 3K9;Q|zĊi¼ĂÑÈ÷° % Af293BM939 U²\üËÅEND,.L¼Ă+óĉ^ÑÈÃ &\üËÅÊËć+ ÿĉ^ÒõvI<LzāÛċä+6Q@( ftmD ĉ^VÂ}ÏÖÓBM939 ²Î® ftmD +q! DNA ¢Ä+ Af293 ²d*DG?MJL8OÊËu ù"&)ëðÑÈæ
FtmD +ÌÊl Ò÷° &Â}Ï% ÿ)(-GBČé%'FtmD ċä¾ÊÉÒ­]R¸ê  _R 'è± Af293 ²DG?MJL8OÊoçĀ FtmD 9>=EĈ¢)(+§& A point mutation of ftmD blocks the fumitremorgin biosynthetic pathway in Aspergillus fumigatus Af293
Naoki Kato1, Hirokazu Suzuki1, Hideo Okumura2, Shunji Takahashi1, Hiroyuki Osada1(1Chemical Biology Dept., RIEKN ASI, 2JASRI)

P-65.A#ÐðYh{^ĆâX%( PK-NRP òoloÅÊËú 66 —ª´ġįÅ­Ĭ®įÈÀÄį°ä×įĢäĈk ĭàí¯íc:[7`P36a>dĮ
éè÷ÞĄ;O\im·rfĂÁ oÓqđĚu
¡+ Ä)  į$/!£ī¤Æs!tëÞ¹*įã+ ¶uęø.¾l+(*įoÓqđĚu .ØµįĂÁ oÓqđĚu .Ø .ÿ+.é¼+poº¶uęø.¾l+(*įxĩÆs!ÚxĩÆs!å+Ý áâď© ÿ+.+ÃÖûÉoº¶uęøgÙ|p38 MAP8KdBOsm1 Ěu îÌ ("įOsm1oº¶uęø HPt oĞĚu îÌ.ãČÇ.ćOsm1Ěu îÌ xĩÆsįY_:E3J-Ĩ_XCd\WUFJĭPK-NRPĮĆÝ+HM1Dbĝ áâ ď©,HM1
Dbĝ!Alternaria¬÷ÞĄö áâ+Z3<I8=b*įEbQ9Ēº.ĥ¥+%»āæØµ áàyã',+Osm1Ěu î(+HM1Dbĝáâď©5S>4NH
2G9 ² ĤĖ.ČÇ+&įğáÌ.R?IbĀ1AF`Ĝùĥ¥ĭSAHAĮDNA]F`ĥ¥ĭ5-azacytidineĮà įáâď©!Ď&),  ûÉ )Osm1Ěu
îÌ!,) Ý(+áâď©!å+]7L@\áâď©,+ ð,Åíò eě!įáíAbEd3OVd=^bnÏ(+¿½.
Induced production of a PK-NRP hybrid compound by disturbance of the two-component signaltransduction system in the rice blast fungus
Choong-Soo Yun, Takayuki Motoyama, Toshiaki Hayashi, Hiroshi Hirota, Hiroyuki Osada(Chem. Biol., RIKEN ASI)

P-66
Üăá÷ÞĄ Epichloë festucae
bQ9Ē Cdc42 ±¦jÍÝ#~ēè¸Ë

+w

Ġ GEÐğĪcõÅĭĦcáĕ¢Į
E. festucae !3NñÜă úþģħºýįáĤz.ïó+÷ÞĄ5bJT03I+ÅĄ!¦jÍÝ įĄ÷}ô!Ą÷ iģ vĢ+į¦jÍÝĐĄ÷ºý.
ÿ+ ,+,%į5bJT03I ØµĝùáºĜù NoxA ¦jáĤz.ïó+&³Ċ+įNoxR w Ġ G EbQ9Ē RacA
NoxA ØµáïóĤh+ Č+noxAįnoxR (" racA îÌ!¦jÍÝĘĄ÷ºý.ðÍÝ.ÊÔ% &į¦jÍÝ ĐĄ÷áý³Ċ+ü ),+,%įRacA Ĭêµ.ð Cdc42 Òÿ. RacA ÕĔČÇįRacA  Cdc42 ,, NoxR  BemA  å+ Nox ĉxÑº û+įracA îÌ ¸Ë¦jÍÝ!ìÊÔ+ ¨įcdc42 îÌ ¸ËÍÝ!{~áý+.pį
cdc42 îÌ GFP .çßį¦jÍÝ Ą÷áý.čúċ§-įğáÌ!¦jÍÝĐĄ÷ ė« ¦jÍÝ ~ēċ§,+ ¨įcdc42 îÌ!ąēě Ą÷ÂÛ *įąēfě Ą÷ Î,  ûÉ(*įcdc42 îÌ!Ą÷úþ
iģvĢ.ć  į¦jÍÝ# ~ēè¸Ëÿ.+ ð,Function of small G protein Cdc42 in intercalary hyphal growth of fungal endophyte Epichloë festucae in hostplant
Yuka Kayano, Daigo Takemoto (Grad. School Bioagr. Sci., Nagoya Univ.)

P-67
Isolation of a gene involved in the growth inhibition of grass pathogens by fungal grass 67 —endophyte Epichloë festucae
Jennifer Niones, Takushi Hashikawa and Daigo Takemoto (Graduate School of Bioagricultural Sciences, Nagoya
University) While much has been reported on the ability of endophyte to protect its host from pathogens, the mechanisms offungal endophyte in plant protection against pathogens are least understood. In this study, we screened for Epichloë
festucae isolates, which could inhibit the mycelial growth of grass pathogens in axenic culture. One of the isolates,
Ef437, significantly inhibits the mycelial growth of several grass pathogens. Through restriction enzyme-mediatedintegration (REMI) mutagenesis of this isolate, we identify several mutants that have lost its ability to inhibit thepathogen. One of the mutants, 830, contains plasmid insertion in the promoter region of a transcription factor Vib-1.
In Neurospora crassa, Vib-1 is known as a transcriptional regulator that is essential for the expression of the genes
involved in non-self recognition and promotion of cell death. Confocal microscopy of GFP under the control ofvib-1 promoter introduced in E. festucae revealed that expression of vib-1 is induced only in endophyte mycelia,
which has an encounter with the pathogens.

P-68
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Cryptococcus neoformans PMT2 gene is required for DBB staining
Kiminori Shimizu, Yumi Imanishi, Susumu Kawamoto(Medical Mycology Res. Cent., Chiba Univ.)

P-69
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- ŢĈ¿Åx~ß!-32R=á"ķ 
Ä"ŢsuŚ. A. fumigatus 44 á(Ĭ°uŚá *#ĂuŚá) +ŢdsRNA /ěĵ- *,Ţ4 á!M2:32R=kØá/åt¼+.32R="!GUCH?WU +Ţ1.
partitivirusŢ2. ŏwÛò£ĕŢ3. chrysovirus ŏwÛò£ĕ! 2 ŒÁàáŢ4. chrysovirus ŇÑĕŢ- Íù.í\e<W75U;W(Roche ē GS junior)/ĄŢ32R=!9FOŏw!ò£/Ļ'í ŢĪ uŚõ *,32R=JQWá!iĵ/Ĳ¼+.32R=JQWá"32R=kØáŢ¸Âţăĩŉ²ţöňĨ¿ţıyĨ¿!ĳā/ïŅ×» ŢM3= ..!á
/ġðŌÁàŢ32R=JQWá32R=kØá!Ĉ¿/ïŅ·Ó".+!Ģß
-

Isolation and characterization of 4 Mycoviruses in opportunistic pathogen Aspergillus fumigatus
Misa Yahara1, Azusa Takahashi-Nakaguchi2, Hiromitu Moriyama3, Tohru Gonoi2(1Grad. School of Medical and Pharm. Sci., Chiba Univ., 2MMRC, Chiba Univ., 3Tokyo Univ. of Agric. & Tech.)

P-70
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ōg ?W9@A1U8õ$!¾Ą
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2E(Ĉį (Magnaporthe oryzae) "2E!ŒĶĈį,ŢÝį!ŋëç!ĹÕ)Ĉ¿ŗŊ
ōg !Ìğ"ŒĶľŝ-DNA aÝŔvÐ (DSBs) l½ġŃ!X-śčÜėĢ
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Ğōg I-Sce I /Ądûċ DSBs §pĜ!đĖ Ã}- (­Ã 23, 24 ®²)čŏw!
Øü *- DSBs l½è³!ĹÞ/ Ţ!ďķ/ōg ?W9@A1U8õ$¾Ąčŏ
w/ÉĜĤ DSBs !§p *,¯ę (ĝ 4000 bp ~) !ì şză-! ¦Ţčŏw/Ø-ĜĤ"ì!z ø¨Ţ¬ HR l½!z YÔ-/ķt&Ţĝ 15 bp Ŗř silent mutation /ËpŢ/čŏw/Ą-ŢÝį! HR "vÐŎhŇn (ĝ 100 bp)ăÖ/Õ+fY!Ģß +ŢÝį! HR " DSBs *,Ľ§.Ţ9FOs Ï-57BI@7čŏw/œ-Ţ9FO!¢£è¿!
{t! Ò ŀþ- ħ+.ōg ?W9@A1U8õ$!¾Ą"Ţéċōg s
DSBs /§p-!~Ā ĝ 100 mĔ²&YŢjģ-čŏw"ĂýKP=NC/Ą(!~Ā/ĥÉ -/đļ
Artificial DSBs induce various genetic variations and their application in gene targeting in Rice blast fungus
Takayuki Arazoe, Tetsuya Younomaru, Shuichi Ohsato, * Tsutomu Arie, Shigeru Kuwata(Sch. Agric., Meiji Univ.; *Grad. Sch. Agric. Sci., Tokyo Univ. of Agric. & Tech.)P-71
2E(Ĉį/Ą7TPOJ4D:WR!ÑĸiĄú!Ìğ
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Search for the novel molecule target(s) of Chloramphenicol in Magnaporthe oryzae.
Ayaka Nishiwaki, Masataka Inoue, Makiko Goto, Takashi kamakura (Tokyo Univ. of Sci.)
P-72
7L)åą

:E_G5BEYaCéD_N<ğl CBP1ÏāđÄáþķĴ¿ýÒ, Ģvķf¢nbķĤsĲ® ĵÃÞħÞ£`¯ßñĶ7L)åą Magnaporthe oryzae !gÉÚ# rw kë"/.²ÆÛãç2¨³. kë¨³!ķ·ëĊĬ ï¥ķäÑ±¥ ÚÞç?=J[*ķ<E<Y^F<A
 ÉÚâÂ³z+Ė¡/. 1 .  ĔùzÏÌ!ÀìĠz  zÏÌ2đ¼.2èçķkë¨³Ė¡½ ĲæÝÛã±2ðğl:E_G5BEYaCéD_N<ğl CBP1(chitin binding protein 1)2ĪCBP1 Ïā2¶.
(ğlîÇ2pČ ¨Ě2ė$0ÚÞç?=J[ ' .äÑ±i£oĚc ķCBP1 ğlîÇ kë¨³Ü!ĢßÇÐ$įĆmd ÉÚâÂ³z Ö+ĢßÇô&kë¨³Ü ¬(ķCBP1 !ÚÞç?=J[ ĕĘĥe.ÿ,/&ķËďÑ±i£oĚc Ģ±Ç!ÉÚâÂ³zdkë2¨³.
 ķCBP1 ğlîÇ!kë2%3¨³   ,ķCBP1 !ÚÞç?=J[ äÑ±! ï¥+Ė¡/.kë¨³?=J[ĥe.¸×/,PKC Õ±.@5?[=ZB]a[+.kë¨³# ©ĭ Đ j:E_G5
BEYaC Ïā ÊĒ2ĉûÅCBP1 !ùĀ³z.:E_2ă5BE[.u2µï¥ ĕĘ+ PKC úě2Õ±.+kë¨³2Ė¡.ā±ð/
The role of Magnaporthe oryzae Chitin-Binding Protein Genes, CBP1 in Appressorium Differentiation
Sho Yoshida, Misa Kuroki, Yuko Ohno, Yuichi Nakajima, Takashi Kamakura(Dept. of Applied Biological Sci., Tokyo Univ. of Science)

P-73>W;)åą

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þ¾4÷8MC;ó$=I:#0ĜÚunĎ] PWT3, PWT4 $WĞ44æ 30kb \S$Ğ
#ê/ć) #©o0P·Ĉ·z$ĜÚu¦"3=I:ó$8MC;#0ĜÚu¦%sPĜÚunĎ] PAT1 #-´ď10¸.
"0 (Oh et al. 2002) [%PAT1 $sĚ#zģ$H@EM<4Ā(${0'ģPAT1 4+=I:ó Br48
PAT1 4+"8MC;ó Br58 4Yď¢ F1 Ę . PAT1 b»óåġóå 76Q1Ģ4č¯ģBr58 ¬Yę0 #-/ PAT1 jĚ BC1F1 Ęġ80 óåĢ4ai1.48MC;}â (cvĤShokan, Pc-38)#²â 2ģĜÚu¦óå Úu¦óå$jĚË 1:1 #Čy$é¿4+#ģBC1F1 80 óå# 0 Pat1 $Ď]4ÌëģSSR HO9O4Ö bulked segregant
analysis 4÷ģ$ PAT1 JM;HO9O(MGM82, MGM83, MGM224ģMGM433) 4úi80 ó
å ."0 PAT1 jĚĘ4ÖjĚj¾4÷é¿ģ1. PAT1 ĊĒ0Þā1
SSR HO9OMGM82, MGM83, MGM224ģMGM433 %ģZheng et al. (2008) # äQÀò`#H@E
M<10.ģPAT1 %äQÀò`#W0
ß~1
Molecular mapping of PAT1, an avirulence gene of Triticum isolates of Magnaporthe oryzae on oat.
Ryota Mori, Yoshihiro Inoue, Izumi Chuma, Yukio Tosa (Graduate School of Agricultural Science, Kobe Univ.)

P-74§ÀT$+Úó1

jÍ0 AVR-Pia ?MD;Ą%£đ02^ö_È ģÏĆV ģgñ 3ģºÁąė 1 ġ1 rĆĖN¥Õáģ2 rõĖNmõģ3 ­ÕÝĢ
5B+Úó%§À¹#8F7;?O?MD;Ą,ĜÚu¦?MD;Ą4jÍ0 ģ$jÍđ,
5Bc$āă#¤ù"đ"!#${%"1"ģĜÚu¦?MD;Ą AVR-Pia$jÍđ4đ0*ģè³ ?MD; ÒØÜ"®`$a©4Ā(ðó4ÖÛÓè
³ AVR-Pia (rAVR-Pia)4ģ°®¦Ď] Pia 4±5B$ô#»d²â 2ģċµ§w¥Ç$ø ý1'ģrAVR-Pia 4²â Pia 5BôhÔ¡ 24 ¹ē'#°®¦ĔĊĎ] PR12
(Mitsuhashi I. et al., 2008) $ÛÓĂ ā*.1
rAVR-Pia 4lÖa©® AVR-Pia ®`$ÒØ¦4Åÿ$é¿ģ® AVR-Pia ®`%+Úó×½$Ð AVR-Pia 4ÒØÜ#āă'ģECL Î#-Åi1CMA$Ûeđ .
+Úó §À¹#jÍ0 AVR-Pia 4đ 2ģôĝ¶ĠĐ 1g /æ 0.47ng (6.4510-2pmol)
1.$é¿ . rAVR-Pia % Pia 5B#āă10ÉÆĉ4±ģ°®w¥4Ă0
ß~1ü|¦5B§À¹$ AVR-Pia jÍđ đ   Pia 5B §Àk¼$£đ$
AVR-Pia 4āăģ°®w¥4Ă0¸.
"'ģ® AVR-Pia ®` Ð AVR-Pia
4ÒØÜ#āă0.ģ1'ý ěģĜü|¦5B# 0°®w¥Ă¹$
AVR-Pia $p¨, 4ģfÙÀò#-xûq 0xï¦ ß1
A trace amount of AVR-Pia protein is secreted from Magnaporthe oryzae during infection
Yuki Sato1, Toyoyuki Ose2, Ryouhei Terauchi3 and Teruo Sone1(1Graduate school of Agriculture, and 2Research faculty of Pharmacology, Hokkaido Univ., 3 Iwate Biotechnology
Research Center)

P-75
5B+ÚóH5=65K>×½

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Ġ1 ³^ăĖQÌÇqÜĢ2 vóQØñ:P;Rġ
Magnaporthe oryzae chrysovirus1(MoCV1).BĎùÒñ*.B%Òñ£¶ ĢÌëĔ&Àu- ā I.5/.O8*MoCV1  5 ;PD3Ā-++DPČ¿ÔË
*Ģ dsRNA4 ORF -ĈrÔË Č¿ãìĢÅìêu&ãìĀęàu¤Ñ&ĢÌëVîú + ( ÌÇě÷ç-ÎĢORF4 wÉ5P8?M3?ĢdsRNA4
iđ5P8?M3?-++h Č¿-ě Ģãì¨-ö~ftĆ&Ìñãì¨¦ÝĢãì¤Ñ-++ú  ,ĢORF wÉh¸')% dsRNA iđh¸ª')ÌëĔ-Ù  ÌÇě÷ç 18 ®ĒĢ30 ®Ēě Č¿6PFO(++ RNA ¥l Ģ¾X`7R30P6R(Illumina Ú MiSeq)-Î ORF4 h'*Č¿ãìĉaÔËu-ÿ äµĢ{ě®Ē ORF4ĢdsRNA4 iđhČ¿++ÔËďT«QUĕ *ĉa¨þ$(+  dsRNA4 ċn}"+* 5’y 3’UTR ċnYĢĂj&éücćĘ
Ģ" mRNA uĘ*%û´-ć$*
Analysis of growth inhibition mechanism caused by the ORF4 protein of Magnaporthe oryzae chrysovirus1.
Tomoko Ohta1, Syunichi Urayama1, Toshiyuki Fukuhara1, Tsutomu Arie1, Tohru Teraoka1, Azusa
Takahashi-Nakaguchi2, Tohru Gonoi2, Akio Tohe2, Hiromitsu Moriyama1.
(1Dept. Appl. Biol. Sci., Tokyo Univ. of Agric. and Tech., 2MMRC, Chiba Univ.)

P-76
Alternaria alternata N18 ¸ÌëĔ-AK ÀâÍÌy!ėÿ·ā

I.5/.O84CJû´ÞUbĊ 1ĢÏ_ 1ĢÛx§õ 1Ģ°Âs 1Ģ¡ 1ĢÂðØÐè 2ĢgÊS± 2Ģºøe 1
Ġ1 ăĖQă, 2 ĝzĖQăġ
Alternaria alternata N18 ¸AHP@7Òx¢, ìÔï® AK Àâ(>1?N0Pč08=O)mÃ £¶óğ©ÓÈ- ā N18 ¸[²Đ RNA -4CJ*I.5/.O8£¶),/.O8£¶¸/.O8ENRu¸Á ĚôÌëĔ-Ù/.O8£¶¸ENRu¸Òx¢-»  ,,£¶¸ENRu¸')%Æ Ò©-@7ó ā Z
ñÌëĔ- ā |-ÖÕ, N18 ¸I.5/.O8 ċn-û´ ²/.O
8 5 mß4CJ-° , RdRp Ę *máåû´äµ,.B%ÒñÀuI.5/.O
8 MoCV1(Magnaporthe oryzae chrysovirus 1)Ąæ* o¬ ", RNA 2  ORF ;PD3Ā
, MoCV1 ZÌëĔ- ā  ORF4 ;PD3ĀĜ×|¢-Ù  (, ;PD3Ā N18 ¸ÌëĔēW * Ù + (/.O8£¶ AK ÀâÍÌ
ďy!ė-ÿ *$ĢHPLC -Î/.O8£¶¸ENRu¸mÌÔïÅY AK Àâď-ý#*

Sequence analysis of mycovirus attenuating A.alternata strain N 18 and analysis of AK toxin production
Kana Takeshita1, Ryo Okada1, Toshiyuki Fukuhara1, Tsutomu Arie1, Tohru Teraoka1, Mayumi Egusa2, Motoichiro
Kodama2, Hiromitsu Moriyama1(1Faculty of Agric., Tokyo Univ. of Agric.& Tech. 2 Faculty of Agric., Tottori Univ.)

P-77
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SIX4 in the cabbage yellows fungus plays different role than in the tomato wilt fungus
Takeshi Kashiwa, Keigo Inami1, Masashi Fujinaga2, Hideki Ogiso2, Tohru Teraoka, Tsutomu Arie
(Grad. Sch. Agric. Sci., Tokyo Univ. of Agric. & Tech., 1Present address: Central Res. Bridgestone Corp., 2Nagano
Veg. and Ornam. Crop. Exp. Stn. )
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Spindle Position Checkpoint (SPOC) component in Saccharomyces cerevisiae is involved in proper cell cycle
progression during appressorium development in Colletotrichum orbiculare
Fumi Fukada1), Ayumu Sakaguchi2), Yasuyuki Kubo1)(1)Life and Environmental Sciences, Kyoto Prefectural Univ., 2)National Institute of Agrobiological S

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Functional analysis of small GTPases, CoCdc42 and CoRac1, from Colletotrichum orbiculare.
Kawashimo Midori, Kozen Yuka, Nomura Takumasa, Kubo Yasuyuki, Tsuji Gento (Grad.sch.of Life and Environ.
Sci.of Kyoto Prefectural Univ.)

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Identification of AF-toxin biosynthetic genes cluster encoded by the 1.0-Mb chromosome in the strawberry
pathotype of Alternaria alternata
Ayumi Hara1, Hikari Kondou1, Yoshiaki Harimoto1, Chiaki Mase1, Yusuke Cho1, Mikihiro Yamamoto2, Kazuya
Akimitsu3, Takashi Tsuge1(1Grad. Sch. Bioagric. Sci., Nagoya Univ., 2Fac. Agr., Okayama Univ., 3Dept. Agr., Kagawa

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Identification of AM-toxin biosynthetic gene cluster encoded by the 1.3-Mb chromosome in the apple
pathotype of Alternaria alternata
Megumi Kawase, Chiho Goto, Yoshiaki Harimoto, Motoichiro Kodama, Mikihiro Yamamoto, Hiroshi Otani,
TakashiTsuge(Univ. of Nagoya, Univ. of Tottori, Univ. of Okayama)

P-82
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9W4YÒÂĝĊ³Đv§/ĚÍd,Fungicide resistance monitoring using pyrosequencing in cucumber corynespora leaf spot fungus
Corynespora cassiicola
Tsuyoshi Shimpuku, Shinpei Banno, Yohei Ishigami, Akihiko Ichiishi, Makoto Fujimura(Fac. of Life Sci., Toyo Univ.)

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The abilities of effectors, secreted by Colletotrichum orbiculare, to induce or suppress plant cell death andtheir subcellular localization during infection
Hiroki Irieda, and Yoshitaka Takano (Grad. School of Agriculture, Kyoto Univ.)

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Colletotrichum orbiculare selects appropriate entry modes via PacC-dependent environmental recognition onplant surface
Kae Yoshino, Yoshitaka Takano (Grad. School of Agriculture, Kyoto Univ.)

P-85
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C5KO:<&ĆÄæą!6RCH4=R ¾®áÑ­ĭ. º, Autophagy is involved in pathogenicity and sexual reproduction in Cochliobolus heterostrophus
Takuya Sumita, Kosuke Izumitsu, Atsushi Morita, Chihiro Tanaka(Grad. School of Agriculture, Kyoto Univ.)

P-86
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Characterizations of PKA genes and PKR gene of Cochliobolus heterostrophus
Satoshi Yutani, Kousuke Izumitsu, Takuya Sumita, Yuuki Kitade, Chihiro Tanaka(Grad. School of Agriculture, Kyoto Univ. )P-87
Cryphonectria parasitica

C ¿ôĀĔb~Ğf2Í
 77 —gyĤ GTP ûAPF8ĔRAS3 Ãı´{uĳŐŊáÉgŐćĐ Ŏ©¦]ňõÝ]óŏ7XĘÙúŎchestnut blightŏŐdø` Ûîú_ÒŐïĊ3KWA3ÍÞ'u7XŎAmerican chestnutŏ1 dĎŀj¨ayë-#ġª1(+čðĜ Cryphonectria parasitica Ő1900 ·Ņ0  +GU^V^7!İînþĄx.0.-ñŐC. parasitica :IRĨ×#$đh,Ő%%ħ¹ +Ûî!
úÀûñS6G>R Ĩ×.Ő%~¿Íæ Üĩ.-C. parasitica r}¤ľ GTP Ē@\K7ı RAS ŁŐ .% RAS1ŐRAS2 ĂĬ.
- ŐÕĀąÌĤ|. RAS3 ßĶ]âęĨ×1ğRAS3 m Ras Łķ@\K7ı#$zĵĬ'+.- C ÔĆņL2YH<YĻqŎCAAX boxŏ1ãïÀŐìî Rin * Rit zĵÃ Ĭ'+.RAS3 1;^F- ras3 ĺp¤āĹûñ1ğŐï ras3 ãÊù ĜčÆĿ)}ô¤»Æ$¼ń1ÜĩGFP Ğ RAS3
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Properties of Cryphonectria parasitica small GTP-binding protein RAS3 which lacks C-terminal lipidation
site.
Yuki Yamauchi, Takuya Takahashi and Shin Kasahara (Dept. of Env. Sci., Miyagi Univ.)P-88

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Aspergillus oryzae.
Nao Imaru1, 2, Fumiko Senoo1Ő2, Shiho Terado1, 2, Yuriko Ikeda2, Kazuhiro Iwashita1, 2(1 Hiroshima Univ., 2 NRIB) 78 —

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 81 —糸状菌分子生物学研究会
1.

会則

本会を糸状菌分子生物学研究会（Fungal Molecular Biology Society of Japan）と呼ぶ。また本会が開く研究会を糸状菌分子生物学コンファレンス（Conference on Fungal Genetics and Molecular
Biology）と呼ぶ。

2.

本会は糸状菌の分子生物学、細胞生物学、生化学、生理学、遺伝学などの普及発展を目的とする。

3.

本会はその目的を達成するために次の事業を行う。
2.

研究会及び総会の開催。

3.

会報の発行。

4.

関連研究団体との協力事業。

5.

その他、必要と思われる事業。

4.

本会はその目的に賛同して入会した個人会員及び総会において承認された名誉会員を持って構成する。

5.

本会入会希望者は所定の入会申込書を提出し、別に定める入会金を納入するものとする。

6.

本会はその運営のため、会長、運営委員若干名および会計監査 1～2 名をおく。任期は 2 年とし、改選は運営委員の推薦と総会の承認による。
(1)

会長は本会を代表し、会務を統括する。

(2)

運営委員は運営委員会を構成し会務を審議する。運営委員には庶務、会計、編集担当、広報担当
をおく。

(3)

本会の会計事務局は会長が指名する会計担当の運営委員の所属する施設に置く。

(4)

会計監査は本会の会計を監査する。

7.

本会は事業運営に必要な実費を年会費として個人会員から徴収する。

8.

本会の事務年度は 7 月 1 日から 6 月 30 日までとする。

9.

前事務年度の庶務、会計については、これを総会において報告し、承認を得るものとする。

10. 本会則の改定には総会出席者過半数の賛成を必要とする。
以上
補則
(1)

本会則は 2001 年 7 月 1 日より発効する。

(2)

本会入会金は 1,000 円とする。

(3)

年会費は一般会員 2,000 円、学生会員 1,000 円とする。

(4)

研究会の通知及び会報は、当該年度までの会費を納入した会員に送付する。

(5)

2 年度にわたって会費納入のない会員は、その資格を失うものとする。

(6)

研究会の発表者は、会員に限るものとする。新入会員の演題申し込みは会費納入の確認を持って受理する。

（平成 23 年 11 月 16 日改正） 82 —2012  v
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