43

沼津工業高等専門学校研究報告
第 51 号，2017 年 1 月

೺ᗣⲔࡢᢠ㓟໬స⏝ⰾ㔝ᜤኈ *1࣭ⰾ㔝ᗈ㉳ *1࣭໭ᮧ⣖ே *2࣭ᚋ⸨ ❳ *2࣭▼⏣཭ᾏ *1
Antioxidant Activities of Various Health Beneficial Teas
Kyoji YOSHINO *1, Hiroki YOSHINO *1, Norito KITAMURA *2,Ryu GOTO *2, Yumi ISHIDA *1
Abstract: In this study, we investigated antioxidant activities of nine kinds of health beneficial
teas produced from the leaves of ashitaba, persimmon, kuma bamboo grass, mulberry, loquat,
green tea and rooibos, and seeds of black soybean and Job's tears. The scavenging activities ofashitaba tea, persimmon tea, mulberry tea, loquat tea and green tea on 1,1-diphenyl-2picrylhydrazyl radical were high as compared to those of the other teas. No correlation was
found between the activities and the contents of total polyphenols, then various antioxidant
components would function in these health beneficial teas.
Key Words:

Tea, Antioxidant activity, Polyphenol䠍䠊䛿䛨䜑䛻Ⲕࡣࢶࣂ࢟⛉ࡢᖖ⥳ᶞ࡛࠶ࡿࢳࣕ㸦Camellia sinensis㸧ࡢⴥ࠿ࡽసࡽࢀࠊୡ⏺୰࡛ᦤྲྀࡉࢀ࡚࠸ࡿ㣧ᩱ࡛࠶ࡿࠋⲔ
ࡣ㏻ᖖࠊ〇㐀ࡉࢀࡓⲔⴥ࡟⇕‫ࢆ‮‬ຍ࠼࡚↦ࡌࡓࡶࡢࡀᦤྲྀࡉࢀࠊࡑࡢ᪉ἲࡣ⡆౽࡛࠶ࡿࠋࡑࡢࡓࡵࠊ௚ࡢ᳜≀࡟ࡘ࠸

var. ma-yuen㸧[5,6,12,15,16]ࠊࣅ࣡㸦Eriobotrya japonica㸧[5,17]ࠊࣝ࢖࣎ࢫ㸦Aspalathus linearis㸧[3,5,18-20]ࡢ 9✀ࡢᕷ㈍ࡢ೺ᗣⲔ࡟ࡘ࠸࡚ẚ㍑ࢆ⾜ࡗࡓࠋ䠎䠊ᮦᩱ䛚䜘䜃᪉ἲ࡚ࡶྠᵝࡢ᪉ἲ࡛ᦤྲྀࡍࡿሙྜࠊⲔ࡜࠸࠺ྡ⛠ࢆ⏝࠸ࡿࡇ㸰㸬㸯 ೺ᗣⲔࡢヨᩱ࡜ࡀከ࠸ࠋ୺࡟೺ᗣಖᣢࡢ┠ⓗ࡛౑⏝ࡍࡿࡑࡢࡼ࠺࡞㣧ᩱ ᕷ㈍ࡢ೺ᗣⲔ࡜ࡋ࡚ࠊ࢔ࢩࢱࣂⲔ㸦A ♫, ࢖ࣥࢻࢿࢩ࢔ࢆⲔእⲔ࡜⛠ࡍࡿࡇ࡜ࡶ࠶ࡿࠋ⏘㸧ࠊ࢝࢟Ⲕ
㸦B ♫, ᪥ᮏ⏘; C ♫, ி㒔ᗓ࠾ࡼࡧ㟷᳃┴⏘㸧ࠊάᛶ㓟⣲ࡸ⸆≀࠿ࡽ⏕ᡂࡍࡿࣛࢪ࢝ࣝศᏊ࡞࡝࡟ࡼࡗࢡ࣐ࢨࢧⲔ㸦D ♫, ᪥ᮏ⏘㸧ࠊࢡ࣐࣓ࣟⲔ㸦E ♫, ୰ᅜ⏘㸧ࠊ࡚యෆࡢ㓟໬ࢫࢺࣞࢫࡀ㧗ࡲࡿࡇ࡜ࡣࠊ⣽⬊ࡀᦆയࡋ࡚ࣜ

ࢡ࣡Ⲕ㸦F ♫, ୰ᅜ⏘㸧ࠊࣁࢺ࣒ࢠⲔ㸦G ♫, ᪥ᮏ⏘㸧ࠊࣅ࣮࣐ࣗࢳࠊ⫢⅖ࠊࡀࢇࠊື⬦◳໬ࠊ࢔ࣝࢶࣁ࢖࣐࣮ᆺ⑵࿈

࣡Ⲕ㸦H ♫, ୰ᅜ; I ♫, ᪥ᮏ⏘; J ♫, ༡஑ᕞ⏘㸧ࠊ⥳Ⲕ㸦K࡜࠸ࡗࡓ⚄⤒㞀ᐖ➼ࠊᵝࠎ࡞⑌⑓ࡀ㉳ࡇࡿཎᅉ࡜࡞ࡿ[1]ࠋ♫, 㟼ᒸ┴⏘㸧ࠊࣝ࢖࣎ࢫⲔ㸦L ♫, ༡࢔ࣇࣜ࢝⏘㸧ࡢ 9 ✀ᢠ㓟໬స⏝࡜ࡣࠊάᛶ㓟⣲ࡸྛ✀ࣛࢪ࢝ࣝࢆᤕᤊࡍࡿࡇ࡜

12 ♫ࡢ〇ရࢆ⏝࠸ࡓࠋⲔࡢ〇㐀࡟⏝࠸ࡽࢀ࡚࠸ࡿ㒊఩ࡣࠊ࡛㓟໬ࢫࢺࣞࢫࢆ㍍ῶࡉࡏࡿຠᯝࢆᣦࡍࠋࢡ࣐࣓ࣟⲔ࡜ࣁࢺ࣒ࢠⲔ࡛ࡣ✀Ꮚࠊࡑࢀ௨እࡢ೺ᗣⲔ࡛ࡣᮏ◊✲࡛ࡣࠊಖ೺స⏝ࡢᣦᶆ࡜ࡋ࡚ࡇࡢᢠ㓟໬స⏝ࢆ⏝ⴥ࡛࠶ࡿࠋ࠸ࠊࡇࢀࡲ࡛࡟ᢠ㓟໬స⏝ࢆ♧ࡍࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿ᳜
≀࡜ࡋ࡚ࠊࢳࣕ[2-6]࡟ຍ࠼࡚࢔ࢩࢱࣂ㸦Angelica keiskei㸧㸰㸬㸰 ೺ᗣⲔࡢᢳฟᾮ  ࡢㄪ〇[6,7]ࠊ࢝࢟㸦Diospyros kaki㸧[3,4,6,8-10]ࠊࢡ࣐ࢨࢧ㸦Sasa ᕷ㈍ࡢ೺ᗣⲔ࡟ࡘ࠸࡚ࠊࡑࢀࡒࢀࡢ 1 g ࡟⇕Ỉ 100 mLveitchii㸧[4,5,11]ࠊࢡ࣐࣓ࣟ㸦Glycine max㸧[12,13]ࠊࢡ

ࢆຍ࠼࡚ 5 ศ㛫ᨺ⨨ࡋࡓᚋࢁ㐣ࡋ࡚ࠊࡑࢀࡒࢀࡢᢳฟᾮࢆ

࣡㸦Morus bombycis㸧[6,14]ࠊࣁࢺ࣒ࢠ㸦Coix lacryma-jobi

ㄪ〇ࡋࡓࠋ*1 ≀㉁ᕤᏛ⛉㸰㸬㸱 ೺ᗣⲔࡢᢳฟᾮ  ࡢㄪ〇

Department of Chemistry & Biochemistry ᕷ㈍ࡢ೺ᗣⲔࡢ୍㒊࡟ࡘ࠸࡚ࠊၟရ࡟⾲グࡉࢀ࡚࠸ࡿ᪉

*2 ᶵᲔᕤᏛ⛉ἲ࡛ᢳฟᾮࢆㄪ〇ࡋࡓࠋලయⓗ࡟ࡣࠊA ♫ࡢ࢔ࢩࢱࣂⲔ࡛

 Department of Mechanical Engineering

ࡣࠊ1 g ࡢⲔ࡟⇕Ỉ 500 mL ࢆຍ࠼࡚ᙅⅆ࡛ 2.5 ศ㛫ຍ⇕

44

沼津工業高等専門学校研究報告ࡋࡓᚋࢁ㐣ࡋࡓࠋB ♫ࡢ࢝࢟Ⲕ࡛ࡣࠊ1.5 g ࡢⲔ࡟⇕Ỉ 200AmL ࢆຍ࠼࡚ 4 ศ㛫ᨺ⨨ࡋࡓᚋࢁ㐣ࡋࡓࠋD ♫ࡢࢡ࣐ࢨࢧ

B

Ⲕ࡛ࡣࠊ5 g ࡢⲔ࡟ 300 mL ࡢ⇕Ỉࢆຍ࠼࡚ᙅⅆ࡛ 3 ศ㛫Cຍ⇕ࡋࡓᚋࢁ㐣ࡋࡓࠋE ♫ࡢࢡ࣐࣓ࣟⲔ࡛ࡣࠊ10 g ࡢⲔ࡟Dศ㛫ຍ⇕ࡋࡓᚋࢁ㐣ࡋࡓࠋG ♫ࡢࣁࢺ࣒ࢠⲔ࡛ࡣࠊ7 g ࡢⲔ࡟⇕Ỉ 1 L ࢆຍ࠼࡚ 2.5 ศ㛫ຍ⇕ࡋࡓᚋࢁ㐣ࡋࡓࠋH ♫ࡢࣅ࣡Ⲕ࡛ࡣࠊ5 g ࡢⲔ࡟⇕Ỉ 1 L ࢆຍ࠼࡚ 5 ศ㛫ᨺ⨨ࡋࡓᚋࢁ㐣ࡋࡓࠋK ♫ࡢ⥳Ⲕ࡛ࡣࠊ2.5 g ࡢⲔ࡟ 80Υࡢ Ỉ200 mL ࢆຍ࠼࡚ 1 ศ㛫ᨺ⨨ࡋࡓᚋࢁ㐣ࡋࡓࠋL ♫ࡢࣝ࢖࣎ࢫⲔ࡛ࡣࠊ1.5 g ࡢⲔ࡟⇕Ỉ 1 L ࢆຍ࠼࡚ᙅⅆ࡛ 4 ศ㛫ຍ⇕ࡋࡓᚋࢁ㐣ࡋࡓࠋ೺ᗣⲔ⇕Ỉ 600 mL ࢆຍ࠼࡚ᙅⅆ࡛ 5 ศ㛫ຍ⇕ࡋࡓᚋࢁ㐣ࡋࡓࠋF ♫ࡢࢡ࣡Ⲕ࡛ࡣࠊ2 g ࡢⲔ࡟⇕Ỉ 1 L ࢆຍ࠼࡚ᙅⅆ࡛ 4

第 51 号

EFGHIJKL0㸰㸬㸲 ࢔ࢰࣛࢪ࢝ࣝᤕᤊຊࡢ ᐃ[21]0.04 M ࣜࣥ㓟⦆⾪ᾮ㸦pH7.4㸧850 PL ࡟ྛⲔᢳฟᾮ

2040DPPHᤕᤊ⋡(%)ᅗ 1 ྠ୍᮲௳࡛ㄪ〇ࡋࡓྛ✀೺ᗣⲔᢳฟᾮࡢᢠ㓟໬స⏝50 PL ࢆຍ࠼ࠊࡉࡽ࡟ 0.5 mM 1,1-ࢪࣇ࢙ࢽࣝ-2-ࣆࢡࣜࣝᖹᆒ್±ᶆ‽೫ᕪ㸬N=3㸬ࣄࢻࣛࢪࣝ㸦DPPH㸧࢚ࢱࣀ࣮ࣝ⁐ᾮ 100 PL ࢆຍ࠼࡚᧠೺ᗣⲔࡢ✀㢮 A㹼L ࡣ͂ᮦᩱ࠾ࡼࡧ᪉ἲ̓ࢆཧ↷㸬ᢾࡋ┤ࡕ࡟㐽ගࡋࡓࠋ20 ศ㛫ᨺ⨨ࡋࡓᚋࠊ཯ᛂᾮࡢ 525nm ࡟࠾ࡅࡿ྾ගᗘࢆ ᐃࡋࡓࠋᑐ↷࡜ࡋ࡚ࡣࠊᢳฟᾮࡢ

Ⲕ࡟ẚ㍑ࡋ࡚ࠊ࢔ࢩࢱࣂⲔࠊ࢝࢟Ⲕࠊࢡ࣡Ⲕࠊࣅ࣡Ⲕࠊ⥳௦ࢃࡾ࡟Ỉࢆຍ࠼ࡓࡶࡢࢆ⏝࠸ࡓࠋୗᘧࢆ⏝࠸࡚ࠊDPPHⲔ࡛ᙉ࠸ຠᯝࡀぢࡽࢀࡓࠋྠ✀ࡢ᳜≀ࡢ୰࡛ࡣࠊ࢝࢟Ⲕࡣࡢ࢔ࢰࣛࢪ࢝ࣝ࡟ᑐࡍࡿᤕᤊ⋡ࢆ⟬ฟࡋࡓࠋᐇ㦂⤖ᯝࡣ 2B ♫࡜ C ♫࡛ຠᯝ࡟ᕪࡣぢࡽࢀ࡞࠿ࡗࡓࡀࠊࣅ࣡Ⲕࡢሙྜ

ᅇ࠶ࡿ࠸ࡣ 3 ᅇࡢᖹᆒ್sᶆ‽೫ᕪ࡛⾲グࡋࡓࠋ࡟ࡣࠊI ♫࡜ J ♫࡟ẚ㍑ࡋ࡚ H ♫࡛ࡣຠᯝࡀప࠸ഴྥࡀぢ

DPPH ᤕᤊ⋡[%]㸻OD A  ODBu 100
OD Aࡽࢀࡓࠋࡇࢀࡽࡢᢳฟᾮ(1)୰ࡢ⥲࣏ࣜࣇ࢙ࣀ࣮ࣝྵ㔞ࢆᐃࡋࡓ⤖ᯝࢆᅗ 2 ࡟♧ࡍࠋ⥳Ⲕࡢ⥲࣏ࣜࣇ࢙ࣀ࣮ࣝ㔞ࡀከODA : ᑐ↷ࡢᐇ㦂⣔ࡢ྾ගᗘࡃࠊḟ࡟࢔ࢩࢱࣂⲔࠊ࢝࢟Ⲕࠊࣅ࣡Ⲕࠊࣝ࢖࣎ࢫⲔ࡛ከ࠿ODB : Ⲕᢳฟᾮࡢᐇ㦂⣔ࡢ྾ගᗘࡗࡓࠋࡑࢀࡒࢀࡢⲔᢳฟᾮ(1)ࡢᢠ㓟໬ຊ࡜⥲࣏ࣜࣇ࢙ࣀ࣮ࣝ㔞ࡢ┦㛵ಀᩘࡣJ=0.43 ࡛ࠊ᫂☜࡞┦㛵ᛶࡣぢࡽࢀ࡞࠿ࡗ㸰㸬㸳 ࣇ࢛࣮ࣜࣥ㸫ࢳ࢜࢝ࣝࢺ࣮ἲ࡟ࡼࡿ⥲࣏ࣜࣇ࢙ࣀࡓࠋࡇࡢࡇ࡜ࡣࠊ௒ᅇ⏝࠸ࡓ೺ᗣⲔ࡟ࡣ࣏ࣜࣇ࢙ࣀ࣮ࣝ௨࣮ࣝ㔞ࡢ ᐃ[22,23]እࡢᢠ㓟໬ᡂศࡀྵࡲࢀࡿࡇ࡜ࢆ♧၀ࡋ࡚࠸ࡿࠋࡇࢀࡲ࡛ ྛⲔᢳฟᾮࡢ 70 PL ࡟ࠊ࿴ග⣧⸆ᕤᴗ♫〇ࣇ࢛࣮ࣜࣥ㸫ࡢሗ࿌࡛ࠊࡑࢀࡒࢀࡢ᳜≀୰࡟ྵࡲࢀࡿᢠ㓟໬ᡂศ࡜ࡋ࡚ࠊ

ࢳ࢜࢝ࣝࢺ࣮ヨ⸆ࡢ 50㸣Ỉ⁐ᾮ 70 PL ࢆຍ࠼࡚ΰྜᚋࠊ3࢔ࢩࢱࣂⴥ࡛ࡣDࢺࢥࣇ࢙࣮ࣟࣝ[24]ࠊ࢝ࣟࢸࣀ࢖ࢻ㸦࢝ศ㛫ᐊ ᨺ⨨ࡋࡓࠋࡇࢀ࡟ 10㸣Ⅳ㓟ࢼࢺ࣒ࣜ࢘Ỉ⁐ᾮࡢࣟࢸࣥ[24]㸪ࣝࢸ࢖ࣥ[25]㸧
ࠊ࢝ࣝࢥࣥ㸦4íࣄࢻࣟ࢟ࢩࢹࣜ

70 PL ࢆຍ࠼࡚ΰྜࡋࠊ1 ᫬㛫ᐊ ᨺ⨨ࡋࡓᚋࠊ650 nm ࡟

ࢩࣥ[7]ࠊ࢟ࢧࣥࢺ࢔ࣥࢤ࣮ࣟࣝ[7]㸧ࡀࠊ࢝࢟ⴥ࡛ࡣࣇࣛ࠾ࡅࡿ྾ගᗘࢆ ᐃࡋࡓࠋ࣏ࣜࣇ࢙ࣀ࣮ࣝ㔞ࡣࠊἐ㣗Ꮚ㓟࣎ࣀ࢖ࢻ㸦࢔ࢫࢺࣛ࢞ࣜࣥ[6,8,26]ࠊ࢖ࢯࢡ࢚ࣝࢩࢺࣜࣥ

࢚ࢳࣝ┦ᙜ㔞࡛⟬ฟࡋࡓࠋᐇ㦂⤖ᯝࡣ 3 ᅇࡢᖹᆒ್sᶆ‽[8,27,28]ࠊ(+)-࢝ࢸ࢟ࣥ[8]㸧
ࠊ࢔ࢫࢥࣝࣅࣥ㓟[6,26]ࡀࠊࢡ

೫ᕪ࡛⾲グࡋࡓࠋ

࣐ࢨࢧⴥ࡛ࡣࣇࣛ࣎ࣥ㸦ࢺࣜࢩࣥ[11]ࠊࣝࢸ࢜ࣜࣥ[11]㸧
ࠊࣇࣛ࣎ࣀ࣮ࣝ[5]ࠊCoQ9[11]ࠊCoQ10[11] ࡀࠊࢡ࣐࣓ࣟ✀䠏䠊⤖ᯝ䛚䜘䜃⪃ᐹᏊ࡛ࡣ࢔ࣥࢺࢩ࢔ࢽࣥ[29,30]㸦ࢩ࢔ࢽࢪࣥ 3- ࢢࣝࢥࢩࢻ[13]㸧
ࠊ࢖ࢯࣇࣛ࣎ࣥ[13]ࡀࠊࢡ࣡ⴥ࡛ࡣࣇࣛ࣎ࣀ࢖ࢻ㸦ࢡ࢔ࢩࢱࣂⲔࠊ࢝࢟Ⲕࠊࢡ࣐ࢨࢧⲔࠊࢡ࣐࣓ࣟⲔࠊࢡ࣡Ⲕࠊ

࢚ࣝࢭࢳࣥ[31,32]ࠊࢣࣥࣇ࢙࣮ࣟࣝ[32]ࠊࣝࢳࣥ[31]ࠊ࢔

ࣁࢺ࣒ࢠⲔࠊࣅ࣡Ⲕࠊ⥳Ⲕࠊࣝ࢖࣎ࢫⲔࡢ 9 ✀ 12 ♫ࡢ೺ࢫࢺࣛ࢞ࣜࣥ[31]ࠊࢡ࢚ࣝࢩࢺࣜࣥ[31]ࠊ࢖ࢯࢡ࢚ࣝࢩࢺ

ᗣⲔࡢᢳฟᾮ(1)࡟ࡘ࠸࡚ࠊDPPH ᤕᤊస⏝ࢆ ᐃࡋࡓ⤖ࣜࣥ[31]㸧
ࠊࢡࣟࣟࢤࣥ㓟[32]ࠊ࢝ࣇ࢙࣮㓟[31] ࡀࠊࣁࢺ࣒ᯝࢆᅗ 1 ࡟♧ࡍࠋྠ୍ࡢ᮲௳࡛ㄪ〇ࡋࡓᢳฟᾮࡢᢠ㓟໬సࢠ✀Ꮚ࡛ࡣࣇࣛ࣎ࣀ࣮ࣝ[5]ࠊࢡࣟࣟࢤࣥ㓟[33]ࠊp-ࢡ࣐ࣝ⏝࡛ࡣࠊࢡ࣐ࢨࢧⲔࠊࢡ࣐࣓ࣟⲔࠊࣁࢺ࣒ࢠⲔࠊࣝ࢖࣎ࢫ

㓟[33]ࠊࢥࢽࣇ࢙ࣜࣝ࢔ࣝࢥ࣮ࣝ[34]ࠊࢩࣜࣥ࢞㓟[34]ࠊࣇ

45

健康茶の抗酸化作用

AAB

BCDE

೺ᗣⲔ

೺ᗣⲔ

DF
GHEFGH

IKJ

LK
L

0020

4060

DPPHᤕᤊ⋡(%)

20⥲䝫䝸䝣䜵䝜䞊䝹㔞(%)ᅗ 2 ྠ୍᮲௳࡛ㄪ〇ࡋࡓྛ✀೺ᗣⲔᢳฟᾮࡢ⥲࣏ࣜࣇ࢙ᅗ 3 ␗࡞ࡿ᮲௳࡛ㄪ〇ࡋࡓྛ✀೺ᗣⲔᢳฟᾮࡢᢠ㓟໬సࣀ࣮ࣝ㔞⏝

ᖹᆒ್±ᶆ‽೫ᕪ㸬N=3㸬

ᖹᆒ್±ᶆ‽೫ᕪ㸬N=2㸬

೺ᗣⲔࡢ✀㢮 A㹼L ࡣ͂ᮦᩱ࠾ࡼࡧ᪉ἲ̓ࢆཧ↷㸬೺ᗣⲔࡢ✀㢮 A㹼L ࡣ͂ᮦᩱ࠾ࡼࡧ᪉ἲ̓ࢆཧ↷㸬࢙ࣝࣛ㓟[33,34]ࠊࢩࣜࣥ࢞ࣞࢩࣀ࣮ࣝ[34]ࠊ4-ࢣࢺࣆࣀࣞ

60

ࢩࣀ࣮ࣝ[34]ࠊࣜࢢࢼࣥ㸦࣐࢚ࣘࣀࣜࢻ㸧[34]ࠊ࣏ࣜࣇ࢙

㓟[35]ࠊࢡ࣐ࣟ࢖ࣝ࢟ࢼ㓟[35]ࠊࣇ࢙ࣝࣟ࢖ࣝ࢟ࢼ㓟[35]㸧
ࠊࣇࣛ࣎ࣀ࢖ࢻ㸦ࢼࣜࣥࢤࢽࣥ[35]ࠊࢡ࢚ࣝࢭࢳࣥ[35]ࠊࢣ
ࣥࣇ࢙࣮ࣟࣝ[35]㸧ࡀࠊࢳࣕⴥ࡛ࡣ࢝ࢸ࢟ࣥ[2]ࠊࣇࣛ࣎ࣀ
࣮ࣝ[5]ࠊ࢔ࢫࢥࣝࣅࣥ㓟[36] ࡀࠊࣝ࢖࣎ࢫⴥ࡛ࡣࣇࣛ࣎ࣀ࢖ࢻ㸦࢚࢜ࣜࣥࢳࣥ[20,37]ࠊ࢖ࢯ࢚࢜ࣜࣥࢳࣥ[37]ࠊ࣍
࢚ࣔ࢜ࣜࣥࢳࣥ[20]ࠊࣝࢸ࢜ࣜࣥ[20,37,38]ࠊࣝࢳࣥ[20,37]ࠊ
࢖ࢯࢡ࢚ࣝࢩࢺࣜࣥ[37,38]ࠊࢡ࢚ࣝࢭࢳࣥ[20,37,38]ࠊ࢔
ࢫࣃࣛࢳࣥ[20,37]ࠊࢡࣜࢯ࢚࣮ࣜ࢜ࣝ[37,38]ࠊࣅࢸ࢟ࢩࣥ

䠠䠬䠬䠤ᤕᤊ⋡(%)࣮ࣝ[5]ࠊࣄࢻࣟ࢟ࢩࢩࣥࢼ࣒㓟ㄏᑟయ㸦࢝ࣇ࢙࢜࢖ࣝ࢟ࢼ

B50

ࣀ࣮ࣝ[15]ࡀࠊࣅ࣡ⴥ࡛ࡣࣉࣟࢩ࢔ࢽࢪࣥ[17]ࠊࣇࣛ࣎ࣀ

DH40A
L
F30E

K20G

1000123ⴥ㔜㔞(g/50 mL)[20,37]ࠊ࢖ࢯࣅࢸ࢟ࢩࣥ[20,37]ࠊࢼࣜࣥࢤࢽࣥ[20]ࠊ࢔ࣆ
ࢤࢽࣥ[20]㸧
ࠊࣉࣟࢺ࢝ࢸ࢟ࣗ㓟[38]ࠊࣇࣛ࣎ࣀ࣮ࣝ[5]ࠊࢱ

ᅗ 4 ␗࡞ࡿ᮲௳࡛ㄪ〇ࡋࡓྛ✀೺ᗣⲔᢳฟᾮࡢᢠ㓟໬సࣥࢽࣥ[5]ࡀࡑࢀࡒࢀ▱ࡽࢀ࡚࠸ࡿࠋ

⏝࡜౑⏝ࡋࡓⲔⴥ㔜㔞࡜ࡢ㛵ಀ ྛ✀೺ᗣⲔࡢᢳฟᾮ(2)࡟ࡘ࠸࡚ࠊDPPH ᤕᤊస⏝ࢆ೺ᗣⲔࡢ✀㢮 A㹼L ࡣ͂ᮦᩱ࠾ࡼࡧ᪉ἲ̓ࢆཧ↷㸬ᐃࡋࡓ⤖ᯝࢆᅗ 3 ࡟♧ࡍࠋ␗࡞ࡿ᮲௳࡛ㄪ〇ࡋࡓᢳฟᾮࡢᢠ㓟໬స⏝࡛ࡣࠊࣁࢺ࣒ࢠⲔ௨እࡢ೺ᗣⲔࡢຠᯝࡢᕪࡀᑠࢀࡽࡢⲔ࡛ࡣ⏝࠸ࡿⲔࡢ㔞ࢆㄪᩚࡍࡿࡇ࡜࡛ࠊᢠ㓟໬ຊࢆࡉࡃ࡞ࡗࡓࠋᚑࡗ࡚ࠊྛ೺ᗣⲔ࡟᥎ዡࡉࢀࡓῥࢀ᪉ࢆࡍࡿྠ⛬ᗘ࡟ࡍࡿࡇ࡜ࡀ࡛ࡁࡿࡶࡢ࡜⪃࠼ࡽࢀࡿࠋࡑࢀ࡟ᑐࡋࠊࡇ࡜࡛ࠊࣁࢺ࣒ࢠⲔࢆ㝖ࡁ࡯ࡰྠ⛬ᗘࡢᢠ㓟໬స⏝ࡀᮇᚅ࢝࢟Ⲕ࡜ࣅ࣡Ⲕࡣᑡ㔞ࡢⲔ࡛ᙉ࠸ᢠ㓟໬స⏝ࢆ♧ࡋࠊࣁࢺ࡛ࡁࡿࡶࡢ࡜⪃࠼ࡽࢀࡿࠋࣁࢺ࣒ࢠⲔࡢᢠ㓟໬స⏝ࡣప࠿࣒ࢠⲔࡣⲔࡢ㔜㔞࡟ᑐࡋ࡚≉࡟ᙅ࠸ᢠ㓟໬స⏝ࢆ♧ࡍࡇࡗࡓࠋ୰࡛ࡶᢠ㓟໬స⏝ࡀᙉ࠿ࡗࡓࡢࡣࠊ࢝࢟Ⲕࠊࢡ࣐ࢨ࡜ࡀࢃ࠿ࡗࡓࠋࢧⲔࠊࣅ࣡Ⲕ࡛࠶ࡗࡓࠋྛ೺ᗣⲔࡢ౑⏝㔜㔞࡜ᢠ㓟໬స⏝ ௨ୖࡢ⤖ᯝ࠿ࡽࠊ௒ᅇ᳨ウࡋࡓ 9 ✀ࡢ೺ᗣⲔࡢ୰࡛ࡣࠊ࡜ࡢ㛵ಀࢆᅗ 4 ࡟♧ࡍࠋ࢔ࢩࢱࣂⲔࠊࢡ࣐ࢨࢧⲔࠊࢡ࣐ࣟ≉࡟࢝࢟Ⲕ࡜ࣅ࣡Ⲕ࡛ᙉ࠸ᢠ㓟໬స⏝ࡀㄆࡵࡽࢀࡓࡀࠊࣁ࣓Ⲕࠊࢡ࣡Ⲕࠊ⥳Ⲕࠊࣝ࢖࣎ࢫⲔ࡟ࡘ࠸࡚ࡣࠊⲔࡢ㔜㔞࡜ࢺ࣒ࢠⲔࢆ㝖ࡃ࢔ࢩࢱࣂⲔࠊࢡ࣐ࢨࢧⲔࠊࢡ࣐࣓ࣟⲔࠊࢡᢠ㓟໬ຊࡢ㛫࡟࡯ࡰ୍ᐃࡢ㛵ಀࡀぢࡽࢀࡓࠋࡑࡢࡓࡵࠊࡇ࣡Ⲕࠊ⥳Ⲕࠊࣝ࢖࣎ࢫⲔ࡟ࡘ࠸࡚ࡣࠊ౑⏝ࡍࡿⲔࡢ㔞ࢆቑ

46

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