Thermal Cycling

Bio-Rad Thermal Cyclers

CGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTACAGATCTTA
1
2
3
4
5
6
7
8
9 10 11 12A
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACCGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTGCTTA
B
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTGCT C
CGCTTAGATCTTACCATTGCTTAGATCTTC
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACATAT D
E
CGCTTAGATCTTACCATTGCTTAGATCTTC
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACGCTTAGATCTTACF
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACATC G
CGCTTAGATCTTACCATCGCTTAGATCTTACCATTGCTTAGATCTTCTACACCGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTGC H
CGCTTAGATCTTACCATTG
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACGCTTA
CCATGTTACGATACTGTATGATGCTTAGATCTTCTACAGATCTTA CCATGTTACGATACTGTATCGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTGCTGC
CGCTTAGATCTTACCACGCTTAGATCTTACCATTGCTTAGATCTTCTACACAGCCCA
CGCTTAGATCTTACCATCGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTGCCAT CGCTTAGATCTTACCATCGCTTACGCTTAGATCTTACCATTGCTTAGATCTTCTCTTCTCGC
CGCTTAGATCTTACCATTGCTTAGATCCTGCCCATG
CGCTTAGCGCTTAGATCTTACCATTGCTTAGATCTTCCTATGACGCTCGCTTAGATCTTACCATTGCTTAGATCTTCTACACGCTTAGACGCTTAGATCTTACCATTGCTTAGATCTTCTACACACGC
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTGCTGC
CGCTT
CGCTTAGATCTTACCATTGCTTAGGATCTTA
CGC
CGCTTAGATCTTACTGTATGATGCTTAGATCTTCTACAGATCTTA
CGCTT
CGCTTAGATCTTACCATTGCTTAGATCTTCTACACATTGCTTGCTTAGATACAGATCTTA
1

A

B
C

D
E
F

G
H

Visit bio-rad.com/CyclersMore for more information.

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BIO-RAD THERMAL CYCLERS

PROVENTHERMAL CYCLERS

For more than 35 years, Bio-Rad Thermal Cyclers have continued to deliver
the same reliability and high-quality performance. With its engineering expertise,
Bio-Rad has developed and manufactured robust and efficient thermal cyclers thatscientists not only want but need. These PCR systems offer enhanced usability
with features designed to support academic, commercial, and biopharmaceuticallaboratories in conducting basic and translational research, process development,
and quality control.
2 | BIO-RAD LABORATORIES, INC.

BIO-RAD THERMAL CYCLERS

TIMELINE
OF
BIO-RAD THERMAL CYCLERS
1988

MiniCycler Personal Thermal Cycler

1991

1994

DNA Engine Tetrad Thermal Cycler

MyCycler Personal Thermal Cycler

2005

2008

T100 and C1000 Touch Thermal Cyclers

iCycler Thermal Cycler

2000

2002

MJ Mini Personal Thermal Cycler

DNA Engine Thermal Cycler

1995

1999

DNA Engine Dyad Thermal Cycler

PTC-100 Thermal Cycler

C1000 and S1000 Thermal Cyclers

2011

2023

PTC Tempo Thermal Cycler

BIO-RAD LABORATORIES, INC. | 3

BIO-RAD THERMAL CYCLERS

FAST ANDUNIFORM

PCR run times can be dramatically reduced without giving up
the flexibility, modularity, and gradient capability of Bio-Rad
Thermal Cyclers. With our thermal cyclers, enzymes, and reaction
vessels, you can:Superior Uniformity and Fast Ramping
It is critical that all wells maintain the proper temperature
throughout each PCR step. The PTC Tempo Thermal Cycler
has six independently controlled thermal electric modules (theheating and cooling elements of the thermal cycler) to maintaintight temperature uniformity at all points during a run — evenduring ramping. The patented reduced-mass sample block* heats
and cools more quickly than standard blocks, improving thermal
uniformity and minimizing edge effects.

RELIABLE PCR■■

THERMAL CYCLING

Shorten PCR runs from 1.5 hr to 35 min eliably amplify long (1 kb), longer (8 kb), and extra long (15 kb)
Rtargets 3–4 times faster than with standard protocols
Fast (two-step)
protocolStandard(three-step)
protocol
0

10

20

30

40

50

60

70

80
30

90

S

F

Actual measured run times, min

F

S

F

S

F

S

F

S

F

[
[
[
[
[
[

S

Tm
bp

63
221

65
225

67
226

63
162

65
178

67
172

		
b-actin			b-globin
Fast two-step protocol results are comparable to those generated using
standard protocols. b-actin and b-globin targets were amplified from humangenomic DNA using iTaq DNA Polymerase. Standard (S) protocol, 1.5 hr; fast (F)protocol, 35 min.

Template length■■

35 min
1 kb

Optimize Reactions with Thermal Gradients■ 
Optimized annealing temperatures to improve speed
and specificity of PCR

iProof High-Fidelity DNA Polymerase

2 hr
2 hr

Common high-fidelity polymerase
Taq polymerase■

1.5 hr8 kb

 ultizone temperature control ensures accuracy andM
reproducibility for dependable results
Dynamic ramping keeps incubation times constant hermal gradient feature is available on all Bio-Rad
T
Thermal Cyclers

9.5 hrAnnealing temperature, ºC

5.5 hrM

2 hr 20 min
15 kb

58 60 62 64 66 68 70 72 M

16.5 hr(Failed amplification)

A(71)

For long (1–15 kb) targets, use of iProof High-Fidelity DNA Polymerase reduces
run times three- to fourfold. Targets of 1, 8, or 15 kb were amplified using threedifferent polymerases. A two-step PCR protocol was used with iProof Polymerase;
three-step protocols using the shortest recommended extension times wereused with other polymerases. Because iProof Polymerase requires an annealing
temperature 5–8°C above typical annealing temperatures, two-step protocols often
can be run without redesigning primers.

Primer pair average Tm , ºC

Protocol length

B
(66)

C
(61)

D
(58)

* U.S. patents 7,955,573, 8,367,014, 8,557,196, and 7,632,464.

4 | BIO-RAD LABORATORIES, INC.

Gradient optimization of different
PCR reactions. All reactions
were evaluated in a single run.
Four different primer sets (A, B, C,
and D) were designed and tested
for amplification. Arrows indicate
the annealing temperature thatprovided the highest specificity while
maintaining good yield. Orange box
indicates optimal temperatures.
M, markers; Tm, melting temperature.

BIO-RAD THERMAL CYCLERS

DEPENDABLESERVICE AND SUPPORT
Bio-Rad provides a comprehensive suite of services to ensure that your instruments are cared for and perform to ourstandard of quality, run after run. Our professional, knowledgeable, and experienced technical support specialists
and service engineers are committed to quality and service. From research to regulated environments, Bio-Rad will
help care for your investment and keep your projects in motion. Confirm that your PTC Tempo Thermal Cycler isperforming at its highest accuracy and uniformity with thermal qualification services. Guarantee with documentation
the proper installation and operation of your instrument by taking advantage of Installation Qualification andOperational Qualification Services.
Thermal Qualification Services
To confirm optimal performance of your PTC
Tempo Thermal Cycler, a trained and certified
Bio-Rad service engineer will:■■■ ssess PCR block accuracy, thermalA
uniformity, and instrument ramp rate

Installation Qualification (IQ) and Operational
Qualification (OQ) Services
A certified service engineer will documentthat instruments are installed and functioning
properly and ensure that instruments will
generate reliable and reproducible data.

 omplete a rapid temperature cycling protocol
Cmimicking an actual experiment to confirm
that thermal block setting times are within
specifications■

 rovide an accurate qualification inP
accordance with National Institute ofStandards and Technology (NIST) standards■

Visit bio-rad.com/ThermalValidation
for more information.

■

 aximize instrument uptime with plannedM
instrument qualification services that ensure
your instruments are performing according
to specifications
Increase your confidence with instrument
validation that helps minimize loss of precious
samples and valuable timeMeet regulatory compliance requirements

Visit bio-rad.com/IQOQ for more information.

Expertise35+ years in PCR
Reliability

Thousands of systems installed in 160+ countries
Innovation

Top market leader in PCR

BIO-RAD LABORATORIES, INC. | 5

BIO-RAD THERMAL CYCLERS

COMPATIBLE
PCR PLASTICS
Bio-Rad offers a broad selection of reaction vessels, including 0.5 and 0.2 ml tubes, high-profile and low-profile microplates, and highdensity 384-well plates, allowing you to choose the best vessel for your application. A full selection of reliable sealers is available and canbe selected based on vessel type, cycling application, or storage method. Use the table below to find the best vessels and sealers to use
in your Bio-Rad Thermal Cycler.
Thermal Cycler Compatibility with PCR Plastic Consumables
Tubes

Catalog #384-Well Plate

Individual, High-Profile

Strips, High-Profile

Strips, Low-Profile

Hard-Shell™ Standard

TFI0201, TWI0201

TBSxxxx, TBCxxxx

TLSxxxx

HSP3xxx

PTC Tempo Thermal Cycler

✔

▲

✔

✔

C1000, C1000 Touch, S1000 Thermal
Cyclers

✔

▲

✔

✔

T100, iCycler, MyCycler Thermal Cyclers✔

✔

DNA Engine, DNA Engine Tetrad, DNA
Engine Tetrad 2, DNA Engine Dyad, Dyad
Disciple, PTC-100 Thermal Cyclers

✔

▲

✔

✔

MJ Mini Thermal Cycler✔

▲✔
96- and 48-Well Plates

Catalog #Hard-Shell
Semi-Skirted
High-Profile

Hard-Shell
Skirted
Low-Profile

Hard-Shell
Semi-Skirted
Low-Profile

Multiplate
Unskirted
High-Profile

Multiplate
Unskirted
Low-ProfileiQ
Semi-Skirted
High-Profile

HSS9xxx

HSP9xxx

HSL9xxx

MLPxxxx

MLLxxxx

2239441

PTC Tempo Thermal Cycler

✔

✔

✔

✔

✔

✔

C1000, C1000 Touch,
S1000 Thermal Cyclers

▲

✔

▲

▲

▲

▲

T100 Thermal Cycler✔

DNA Engine, DNA Engine Tetrad,
DNA Engine Tetrad 2, DNA Engine Dyad,
Dyad Disciple, PTC-100 Thermal Cyclers▲

iCycler Thermal Cycler

✔

▲
✔

▲

▲

MyCycler Thermal Cycler
MJ Mini Thermal Cycler

▲

▲

✔

▲

✔

▲

▲

✔

PCR Plate Seals and Cap Strips

Catalog #Microseal™ 'A' PCR
Plate and PCR
Tube Sealing Film

Microseal 'B'
PCR Plate
Sealing Film

Microseal 'C'
PCR Plate
Sealing Film

Microseal 'F'
PCR Plate
Seal

Domed
8-Cap Strips

Optical Flat
8-Cap Strips

MSA5001

MSB1001

MSC1001

MSF1001

TCS0801

TCS0803

PTC Tempo Thermal Cycler

✔

✔

✔

✔

▲

✔

C1000, C1000 Touch, S1000, T100, iCycler,
MyCycler, DNA Engine, DNA Engine Tetrad,
DNA Engine Tetrad 2, DNA Engine Dyad,
Dyad Disciple, PTC-100 Thermal Cyclers

▲

✔

✔

✔

▲

▲

✔

✔

MJ Mini Thermal Cycler

PCR Plate Sealer and Heat Seals
PCR Plate Heat Seals
Catalog #PX1 PCR Plate Sealer✔ Recommended▲ Compatible

For more information, request bulletin 6090.

6 | BIO-RAD LABORATORIES, INC.

Optically Clear Heat Seal

Permanent Clear Heat Seal

Pierceable Foil Heat Seal

Peelable Foil Heat Seal

1814030

1814035

1814040

1814045

✔

✔

✔

✔

BIO-RAD THERMAL CYCLERS

SYSTEM
SPECIFICATIONS

Thermal cyclers are essential laboratory equipment for scientists performing sequencing, cloning, genotyping, mutagenesis, and manyother applications. Features common to all Bio-Rad Thermal Cyclers include Peltier effect technology, which allows precise temperature
control, and gradient capability with 8 or 16 temperature points for optimizing PCR assays easily in a single run. Instruments range fromthe economical T100 Thermal Cycler to the various models of PTC Tempo Thermal Cyclers.
Specifications
PTC Tempo Thermal Cycler
Key features lexible performance for demanding applications and multiple users
FSecure user mode for enhanced securityn Automation compatibility (96-well, deepwell, and 384-well)

T100 Thermal Cycler
Robust and economical for routine PCRCompact footprint

n

n

n

n

System tour

bio-rad.com/PTCTempoTourVideo

bio-rad.com/T100TourVideo

Block formatsnIndependent blocks: dual 48/48-well (0.2 ml)Faster ramping: 96-well (0.2 ml)n Higher volume: deepwell (0.2 and 0.5 ml)n High throughput: 384-well

96-well (0.2 ml high-profile) utomated: 96-well, deepwell, 384-well
A
Nonautomated: 48/48-well

Nonautomated: 96-well

User interface

8 in. graphical touch screen

5.7 in. graphical touch screen

PCR consumables

Both high- and low-profile tubes and plates

Only high-profile tubes and plates

Thermal accuracy±0.2°C of programmed target at 90°C

±0.5°C of programmed target

Thermal uniformity±0.4°C well-to-well within 10 sec of arrival at 90°C

±0.5°C well-to-well within 30 sec

Ramp raten 6-well: maximum is 5°C/sec; average is 3.3°C/sec
9
Deepwell: maximum is 2.5°C/sec; average is 2°C/secn 
48/48-well: maximum is 4°C/sec; average is 3°C/secn 
384-well: maximum is 2.5°C/sec; average is 2°C/sec

Maximum is 4°C/sec; average is 2.5°C/sec

Temperature range

4–100°C

4–100°C

Thermal gradientnn

Lid type

n
n

n

n

Dimensions (W x D x H)

 perational range: 30–100°C
O
Programmable span:– 96-well, deepwell, 48/48-well: 8 rows, 1–24°C– 384-well: 16 rows, 1–24°Cnn perational range: 30–100°C
O
Programmable span: 8 rows, 1–25°C

28 x 50 x 26 cm(11 x 20 x 10 in.)

26 x 47 x 23 cm(10 x 18 x 9 in.)

 6-well, deepwell, and 384-well: 15 kg (33 lb)
9
48/48-well: 14 kg (31 lb)

9 kg (20 lb)

Communications

WiFi, BR.io, USB-A 2.0, Ethernet, USB barcode scanner

USB-A 2.0

Estimated memory capacity

100,000 files, which include protocols and run reports

500 protocols

Weightnn* Depending on reaction module. Go to bio-rad.com/CyclersMore for complete specifications.

BIO-RAD LABORATORIES, INC. | 7

Ordering Information
Catalog #Instruments
12015382
12015392
12015309
12015394
1814000
Accessories
12018650
12018509
1849000
1849001
12020274
12014294
12019776
12013205
Reagents
172531017253201708870
1708872
1708874
12012802
1708840
1725034
1725037
17088961708890
Plastics
TLS0801
TFI0201
TWI0201
TBS0201
TBC0802
TCS0803
HSP9601
MLL9601
HSL9601
HSL9605
HSL9905
HSS9601
HSS9901
MLP9601
MLL4801
MLP4801
HSP3801
HSP3901
12001925

Description
PTC Tempo 96 Thermal Cycler
PTC Tempo Deepwell Thermal Cycler
PTC Tempo 48/48 Thermal Cycler
PTC Tempo 384 Thermal Cycler
PX1 PCR Plate Sealer
PTC Tempo Thermal Cycler WiFi Adapter, U.S. only
PTC Tempo Thermal Cycler WiFi Adapter, global
Tube Frame for 0.2 ml Tubes, for use with 48/48- and 96-wellreaction modules
Tube Frame for 0.2 ml Tubes, for use with 96–deep well anddeepwell reaction modules
Tube Frame for 0.5 ml Tubes, for use with 96–deep well anddeepwell reaction modules
Plate Lifter for 384-Well Reaction Module
PTC Tempo Thermal Cycler API, 1 license
Ethernet Cable
iProof HF Master Mix, 100 x 50 µl reactions, 2.5 ml
iProof GC Master Mix, 100 x 50 µl reactions, 2.5 ml
iTaq DNA Polymerase, 250 U (5 U/µl), 50 µl
MgCl2 Solution for PCR, 50 mM, 1.25 ml
dNTP Solution Mix, 10 mM, 200 μl
Reliance Select cDNA Synthesis Kit, 25 x 20 µl reactions
iScript Reverse Transcription Supermix, 25 x 20 µl reactions
iScript gDNA Clear cDNA Synthesis Kit, 25 x 20 µl reactions
iScript Advanced cDNA Synthesis Kit, 25 x 20 µl reactions
iScript Select cDNA Synthesis Kit, 25 x 20 µl reactions
iScript cDNA Synthesis Kit, 25 x 20 µl reactions
0.2 ml 8-Tube PCR Strips without Caps, low profile, clear, 120
0.2 ml PCR Tubes with Flat Caps, high profile, clear, 1,000
0.2 ml PCR Tubes with Domed Caps, high profile, clear, 1,000
0.2 ml 8-Tube PCR Strips without Caps, high profile, clear, 125
0.2 ml 8-Tube PCR Strips and Domed Cap Strips,
high profile, clear
0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear
Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted,
white/clear, 50
Multiplate 96-Well PCR Plates, low profile, unskirted, clear, 25
Hard-Shell 96-Well PCR Plates, low profile, semi-skirted,
clear/clear, 25
Hard-Shell 96-Well PCR Plates, low profile, semi-skirted,
clear/white, 25
Hard-Shell 96-Well PCR Plates, low profile, semi-skirted,
clear/white, barcoded, 25
Hard-Shell 96-Well PCR Plates, high profile, semi-skirted,
clear/clear, 25
Hard-Shell 96-Well PCR Plates, high profile, semi-skirted,
clear/clear, barcoded, 25Multiplate 96-Well PCR Plates, high profile, unskirted, clear, 25
Multiplate 48-Well PCR Plates, low profile, unskirted, clear, 50
Multiplate 48-Well PCR Plates, high profile, unskirted, clear, 50
Hard-Shell 384-Well PCR Plates, thin wall, skirted, clear/clear, 50
Hard-Shell 384-Well PCR Plates, thin wall, skirted, clear/clear,barcoded, 50
ddPCR™ 96-Well Plates, semi-skirted, clear/clear, 25

Catalog #Description

MSA5001

Microseal 'A' PCR Plate and PCR Tube Sealing Film,
nonadhesive sealing film, 50
Microseal 'B' PCR Plate Sealing Film, adhesive, optical, 100
Microseal 'C' PCR Plate Sealing Film, adhesive, optical, 100
Microseal 'F' PCR Plate Seal, foil, pierceable, 100
PCR Plate Heat Seal, clear, optical, 100
PCR Plate Heat Seal, foil, pierceable, 100
PCR Plate Heat Seal, foil, peelable, 100

MSB1001
MSC1001
MSF1001
1814030
1814040
1814045
Agaroses
1613102
161310716131121613115

Certified Molecular Biology Agarose, 500 g
Certified Low Range Ultra Agarose, 125 g
Certified Low Melt Agarose, 125 g, for DNA fragments ≥1,000 bp
Certified PCR Low Melt Agarose, 500 g, for DNA
fragments ≤1,000 bp
Certified PCR Agarose, 500 g, for DNA fragments ≤1,000 bp

1613105
Buffers1610770
10x TBE (Tris/Boric Acid/EDTA) Buffer, 5 L
1610733
10x TBE (Tris/Boric Acid/EDTA) Buffer, 1 L
1610773
50x TAE (Tris/Acetic Acid/EDTA) Buffer, 5 L
1610743
50x TAE (Tris/Acetic Acid/EDTA) Buffer, 1 L
1610433
Ethidium Bromide Solution, 10 mg/ml
1610729
EDTA, 500 g, ≥99% pure1610716
Tris, 500 g, ≥99.8% pure
Horizontal Electrophoresis
1704486
Mini-Sub™ Cell GT Horizontal Electrophoresis System
1640300
Mini-Sub Cell GT Horizontal Electrophoresis System andPowerPac Basic Power Supply
1704485
Wide Mini-Sub Cell GT Horizontal Electrophoresis System
1640301
Wide Mini-Sub Cell GT Horizontal Electrophoresis System andPowerPac Basic Power Supply
1704482
Sub-Cell™ GT Horizontal Electrophoresis System
1640302
Sub-Cell GT Horizontal Electrophoresis System and PowerPac
Basic Power Supply
Rulers1708351
EZ Load 20 bp Molecular Ruler, 20–1,000 bp, 50 bands
1708352
EZ Load 100 bp Molecular Ruler, 100–1,000 bp, 10 bands
1708353
EZ Load 100 bp PCR Molecular Ruler, 100–3,000 bp, 30 bands
1708354
EZ Load 500 bp Molecular Ruler, 500–8,000 bp, 16 bands
1708355
EZ Load 1 kb Molecular Ruler, 1–15 kb, 15 bands
1708200
AmpliSize™ Molecular Ruler, 50–2,000 bp, 10 bands
Imaging
12009077
GelDoc Go Gel Imaging System with Image Lab Touch Software,
includes UV/Stain-Free Imaging Tray
1660531
UView Mini Transilluminator

Visit bio-rad.com/CyclersMore for more information.
Bio-Rad PCR reagents and analytical instruments are manufactured
under an ISO 13485:2016 certified Quality Management System andare quality control tested to ensure consistent product performance
you can trust.
BIO-RAD, AMPLISIZE, DDPCR, HARD-SHELL, MICROSEAL, MINI-SUB, and SUB-CELL
are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks usedherein are the property of their respective owner. © 2023 Bio-Rad Laboratories, Inc.
The Mini-Sub Cell GT, Wide Mini-Sub Cell GT, and Sub-Cell GT Cell Systems and/or theiruse are covered by the following U.S. patent and/or pending U.S. patent application or theirforeign counterparts owned by or under license to Bio-Rad Laboratories, Inc., including,but not limited to, U.S. Patent No.8,262,889.

Bio-RadLaboratories, Inc.
Website bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 00 800 00 24 67 23 Belgium 00 800 00 24 67 23 Brazil 4003 0399
Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 00 800 00 24 67 23 Denmark 00 800 00 24 67 23 Finland 00 800 00 24 67 23
France 00 800 00 24 67 23 Germany 00 800 00 24 67 23 Hong Kong 852 2789 3300 Hungary 00 800 00 24 67 23 India 91 124 4029300 Israel 0 3 9636050
Italy 00 800 00 24 67 23 Japan 81 3 6361 7000 Korea 82 080 007 7373 Luxembourg 00 800 00 24 67 23 Mexico 52 555 488 7670
The Netherlands 00 800 00 24 67 23 New Zealand 64 9 415 2280 Norway 00 800 00 24 67 23 Poland 00 800 00 24 67 23 Portugal 00 800 00 24 67 23
Russian Federation 00 800 00 24 67 23 Singapore 65 6415 3188 South Africa 00 800 00 24 67 23 Spain 00 800 00 24 67 23 Sweden 00 800 00 24 67 23
Switzerland 00 800 00 24 67 23 Taiwan 886 2 2578 7189 Thailand 66 2 651 8311 United Arab Emirates 36 1 459 6150 United Kingdom 00 800 00 24 67 23

Life ScienceGroup

Bulletin 5277 Ver J

US/EG

23-04790823

Sig 0123