2012
East and Southeast Asia Biodiversity Information Initiative(ESABII)ESABII-ACB-JAIF

Training Course on
Freshwater/Brackish-water Fish Taxonomy
Completion Report

January 2013

East and Southeast Asia Biodiversity Information Initiative(ESABII)

Contents
Introduction------------------------------------------------------------------------------- 1

１． Summary------------------------------------------------------------------------------- 1

２． Course summary------------------------------------------------------------------------------- 6

２－１

Assembling participants------------------------------------------------------- 6

２－２

Opening ceremony------------------------------------------------------------------- 6

２－３

Museum visit------------------------------------------------------------------- 6

２－４

Lectures on freshwater and brackishwater fish taxonomy

２－５

Practical fish identification with fixed specimens--------------------- 8

２－５－１

Laboratory works

２－５－２

Identification practice with prepared specimens

２－６--------- 7--------------------------------------------- 8--------- 8

Sampling fish in the field (main stream) and photographing fish
specimens------------------------------------------------------------------------------- --9

２－６－１ Capturing fish and treatment of fish specimens in the field ----- 9
２－６－２

Sorting specimens and fin setting------------------------------- 10

２－６－３

Photographing fish specimens------------------------------- 11

２－７

Sampling fish in the field (swift water), identification and preparation ofpresentation------------------------------------------------------------------- 12

２－７－１ Capturing fish and treatment of fish specimens in the field --- 12
２－７－２

Species Identification------------------------------------------- 13

２－７－３

Preparation of presentation------------------------------------------- 13

２－８

Presentation of the results

２－９ Awarding certificate
Conclusion------------------------------------------- 14------------------------------------------- 15------------------------------------------------------------------------------- 15

Introduction
This is to report the completion of the training course on “Freshwater and brackish
water fish taxonomy” as a program for 2012 under East and Southeast Asia Biodiversity
Information Initiative (ESABII), organized by ASEAN Centre for Biodiversity (ACB)
and Ministry of the Environment of Japan (MoEJ).
Japan Wildlife Research Center (JWRC) and Nagao Natural Environment
Foundation (NEF) were jointly entrusted with the task of implementing the training
course.
１．Summary

BackgroundAt the meeting of ESABII, “Inception Workshop and 2nd Project Steering
Committee Meeting for the Expanded Taxonomic Capacity Building andGovernance for Conservation and Sustainable Use of Biodiversity” held in Hanoi,
Vietnam from 5-6 March 2012, freshwater and brackish water fishes were adopted
as a target taxon which would be dealt with in a training course of taxonomy in2012. For the venue of the training course, the Mekong River which runs through 4
countries of Indochina was proposed as the region because of its richness in species
diversity of freshwater and brackish water fishes. After that, Thailand was
proposed and selected as the country where the training course would be
implemented because of the high number of qualified resource persons and training
facility there.

Duration
November 5-10, 2012 (6 days)



Venue


Faculty of Science, Srinakharinwirot University, Bangkok, Thailand(5-6 November)Faculty of Agriculture, Ubon Ratchathani University, Ubon Ratchathani,
Thailand (7-10 November)Participants
Seventeen participants from nine ASEAN countries, and seven from East Asia
participated in the training course (Table 1 and 2). In addition, two voluntary
participants joined the course from Japan (Table 3).
1

Mekong River

ThailandLaos
Mun River
Ubon Ratchathani

Bangkok

Mekong River Basin

Cambodia
Vietnam

Venues:Srinakharinwirot University, Bangkok
Ubon Ratchathani University, Ubon RatchathaniField practice sites:
Mun River, Ubon Ratchathani
Sae Huea MaewUbon RatchathaniTrainers
The Mekong River is an international river that runs through six countries
including four in Indochina (Vietnam, Cambodia, Laos and Thailand), as well as
Myanmar and China, and we could assign trainers on Mekong fishes not only fromThailand but also from neighboring countries including Cambodia, Laos andVietnam. In order to manage and lead 26 participants from various countries
properly, a team of 14 skilled trainers composed of ten from Indochina etc. and four
from Japan were closely taking care of the participants throughout the duration.
See Table 3 for details of trainers.



Organizers
Two officials from respective co-organizers of the training course (MoEJ and
ACB) accompanied the participants and trainers throughout the duration of the
training course, and made opening and closing addresses, and presented the award
certificates. See Table 4 for details of organizers.

Overall program
The overall program was conducted as seen in Table 5.
2

Table 1. Participants
No.
Country
Title

Name

Job title

1

China

Dr.

Wan-Sheng Jiang

Assistant Professor

2
3
4
5
6
7

China
Japan
Japan
Korea
Korea
Mongolia

Dr.
Dr.
Mr.
Mr.
Mr.
Mr.

Xuzhen Wang
Hiroaki Takeuchi
Seigo Kawase
Hyeong-Su Kim
Hyun-Geun Cho
Purevsuren Myagmarjav

Researcher
Postdoctoral Fellow
JSPS Research Fellow
Doctoral course
Researcher
Master course

8

Cambodia

Mr.

Chak Sokhavicheaboth

Chief

9
10
11
12
13
14
15
16

Cambodia
Indonesia
Indonesia
Lao PDR
Lao PDR
Malaysia
Myanmar
Myanmar

Mr.
Mr.
Mr.
Ms.
Ms.
Mrs.
Ms.
Ms.

Hong Lork
Bernadus Agus Hartanto
Hadi Dahruddin
Tick Nouanthavong
Vanida Boualaphan
Normah Said
Khin Mar Myint
L.K.C. Yun

Chief
Director of Biodiversity Conservation
Director of Biodiversity Conservation
Government Officer
Technical Officer
Fisheries Officer
Research Assistant 2
Ranger17 PhilippinesMr.

Al Orolfo

Regional Technical Director

18 Philippines

Mr.

Demosthenes Togonon

Aquaculturist II

19 Singapore

Mr.

Bi Wei Low

Postgraduate Student

20 Singapore

Mr.

How Choon Beng

Section Head (Sungei Buloh Wetland
Reserve)

21 Thailand

Ms.

Jantharas Posombat

22 Thailand

Mr.

Yongyote Reekanong

Fisheries Biologist

23 Vietnam

Mt

Nguyen Dinh Tao

Researcher

24 Vietnam

Ms.

Yan Hai Nguyen

Researcher

Organization
State Key Laboratory of Genetic Resources and Evolution, Kunming
Institute of Zoology, Chinese Academy of Sciences
Institute of Hydrobiology, Chinese Academy of Sciences
Kinki University
Kinki University
Chonbuk National University
National Institute of Biological Resources
National University of Mongolia
International conservation and Biodiversity Department, Ministry ofEnvironment
Wetland and Coastal Zone Department, Ministry of Environment
Rawa Aopa Watumohai National Park, Ministry of Forestry
Research Center for Biology, Indonesian Institute of Science (LIPI)
Living Aquatic Recourse research Center (LARReC)
Living Aquatic Recourse research Center (LARReC)
Department of Marine Parks Malaysia
Forest Research Institute, Forest Department
Ranger, Nature and Wildlife Conservation Division, Forest Department
Protected Areas, Wildlife and Coastal Zone Management Service,
Department of Environment and Natural Resources Region 7
Bureau of Fisheries and Aquatic Resources
Freshwater and Invasion Biology Laboratory, Department of Biological
Sciences, National University of Singapore
National Parks Board
Thailand Natural History Museum, National Science Museum
Inland Fisheries Research and Development Bureau, Department of
Fisheries
Institute of Ecology and Biological Resources (IEBR), Vietnam Academyof Science and Technology
Institute of Science for Environmental Management (ISEM), Viet Nam
Environment Administration, Ministry of Natural Resources andEnvironment

3

Table 2. Voluntary Participants
No.
Country
Title
Name
1 JapanDr.
Tomomi Yamashita
2 JapanDr.
Yuichi Kano

Job title
Assistant Professor
Assistant Professor

Table 3. Trainers
No.
Country
1 Japan2 Japan
3 Japan
4 Japan
5 Australia

Title
Name
Prof. Dr. Yasuhiko TakiDr.
Kenzo Utsugi
Dr.
Koichi Shibukawa
Ms.
Tomoko Oizumi
Mr.
Stefan Ottomanski

Job title
President
Research Scientist
Research Scientist
Research Scientist
Research Scientist

6

CambodiaMr.

Phanara Thach

Head of Laboratory

7
8

Lao PDR
ThailandMr.
Dr.

Bounthob Praxaysombath
Achariya Rangsiruji

Head
Deputy Dean

9

ThailandDr.

Apinun Suvarnaraksha

Assistant Professor

10 Thailand

Mr.

Chaiwut Grudpan

Lecturer

11 Thailand

Dr.

Chavalit Vidthayanon

Senior Aquatic Ecology Specialist

12 Thailand

Ms.

Jarungjit Grudpan

Lecturer

13 Thailand

Dr.

Wichian Magtoon

Dean

14 Vietnam

Dr.

Dinh Dac Tran

Head of Department

Table 4. Organizers
No.
Country

Title

Name

1

Japan

Dr.

Masaya Tatara

2

Japan

Ms.

Mari Takehara

34Philippines
Philippines

Dr.
Ms.

Filiberto Pollisco
Rhia Galsim

Organization
Kyushu University
Kyushu University

Job title
Deputy Director

Program Management Specialist
Capacity Development Officer

4

Organization
Nagao Natural Environment Foundation
Nagao Natural Environment Foundation
Nagao Natural Environment Foundation
Nagao Natural Environment Foundation
Nagao Natural Environment Foundation
Inland Fisheries Research and Development Institute (IFReDI), Fisheries
Administration
Department of Biology, Faculty of Science, National University of Laos
Research and International Affairs, Srinakharinwirot University
Faculty of Fisheries Technology and Aquatic Resources, Maejo
university
Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani
University
Environment Programme, Mekong River Commission
Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani
University
Faculty of Science, Srinakharinwirot University
Fisheries Resources Management, College of Aquaculture and Fisheries,
Can Tho University

Organization
Biodiversity Center of Japan, Nature Conservation Bureau, Ministry of
the Environment
Biodiversity Center of Japan, Nature Conservation Bureau, Ministry of
the Environment
ASEAN Centre for Biodiversity
ASEAN Centre for Biodiversity

Table 5. Overall Program
ESABII - JAIF Training Course on Freshwater / Brackish Water Fish Taxonomy
Da y 1: 5 November (Monda y) - Arri va l of Pa rti ci pa nts a nd ACB Sta ff a t Sri na kha ri nwi rot Uni vers i ty, Ba ngkok
17:30 - 18:00
Regi s tra ti on
18:00 - 19:00
Openi ng Ceremony
Rema rks by As s i s ta nt Prof Dr Cha l ermcha i Boonya l eepun (Pres i dent, SWU)
Rema rks by Dr Ma s a ya Ta ta ra (Deputy Di rector, BIODIC, MoEJ)
Rema rks by Dr Fi l i berto a . Pol l i s co, Jr. (Pol i cy a nd Progra m Devel opment Speci a l i s t, ACB)
Introducti on of ESABII by Ms Ma ri Ta keha ra (BIODIC, MoEJ)
Keynote Speech"Ta xonomy of fres hwa ter fi s hes i n Southea s t As i a : i ts pa s t, pres ent a nd future"
by Prof Dr Ya s uhi ko Ta ki (Pres i dent, NEF)
19:00 - 20:30
Wel come Pa rty
Da y 2: 6 November (Tues da y)
8:00
Depa rture from the Hotel
10:00 - 12:00
Vi s i t the Na tura l Hi s tory Mus eum, Pa thum Tha ni
12:00 - 13:00
Lunch brea k
13:00
Depa rture to the Ba ngkok Ai rport
16:00 - 17:05
Fl i ght from Ba ngkok to Ubon Ra tcha tha ni
Da y 3: 7 November (Wednes da y) - Venue: Ubon Ra tcha tha ni Uni vers i ty, Ubon Ra tcha tha ni
9:00 - 12:00
Openi ng ceremony
Rema rks by Dr Si ri nti p Boonmee (As s i s ta nt to the Pres i dent, Interna ti ona l Rel a ti on, UBU)
Lectures on fi s h ta xonomy (genera l )"Current s ta tus on fres hwa ter/es tua ri ne fi s h bi odi vers i ty i n Southea s t As i a "by Dr Cha va l i t Vi dtha ya non (Seni or Aqua ti c Ecol ogy Speci a l i s t, Envi ronment Progra mme, Mekong
Ri ver Commi s s i on)"Sys tem of na mi ng fi s hes - how to propos e a new s peci es ?"by Dr Koi chi Shi buka wa (Res ea rch Sci ents t, NEF)
"Lerni ng how to prepa re a nd ma na ge fi s h s peci mens " a nd "Pra cti ca l tra i ni ng on fi s h s peci es
i denti fi ca ti on (wi th prepa red s a mpl es )"by Mr Cha i wut Grudpa n (Lecturer, Depa rtment of Fi s heri es , Fa cul ty of Agri cul ture, UBU)
Ms Ja rungji t Grudpa n (Lecturer, Depa rtment of Fi s heri es , Fa cul ty of Agri cul ture, UBU)
12:00 - 13:30
Lunch brea k
14:00 - 17:30
Pra cti ca l tra i ni ng on fi s h s peci es i denti fi ca ti on (wi th prepa red s a mpl es )Lerni ng how to us e keys to s peci es
Lerni ng how to obs erve s a mpl es
Da y 4: 8 November (Thurs da y) - Venue: Ubon Ra tcha tha ni Uni vers i ty, Ubon Ra tcha tha ni
9:00 - 12:00
Fi el dwork on col l ecti ng fi s hes (1)12:00 - 14:00
Lunch brea k
14:00 - 17:30
Speci men prepa ra ti on (fi xa ti on) a nd photogra phi ng
Speci es i denti fi ca ti on of col l ected s peci mens
Da y 5: 9 November (Fri da y) - Venue: Ubon Ra tcha tha ni Uni vers i ty, Ubon Ra tcha tha ni
5:30 - 7:30
Vi s i t morni ng ma rket
7:30 - 12:00
Fi el dwork on col l ecti ng fi s hes (2)Vi s i t the Pha Ta em Na ti ona l Pa rk
12:00 - 14:00
Lunch brea k
14:00 - 17:30
Speci men prepa ra ti on (fi xa ti on) a nd photogra phi ng
Speci es i denti fi ca ti on of col l ected s peci mens
Report wri ti ng
Da y 6: 10 November (Sa turda y) - Venue: Ubon Ra tcha tha ni Uni vers i ty, Ubon Ra tcha tha ni
9:00 - 12:00
Pres enta ti on of reports
Cl os i ng progra m
Concl us i ng rema rks by Dr Ma s a ya Ta ta ra
Concl us i ng rema rks by Dr Fi l i berto a . Pol l i s co, Jr.
Awa rdi ng of works hop certi fi ca tes
12:00 - 13:30
Lunch brea k
14:00 - 17:00
Ci ty tour
19:00 - 22:00
Fa rewel l pa rty
Da y 7: 11 November (Sunda y) - Homebound: pa rti ci pa nts go ba ck to home countri es /ACB s ta ff go ba ck to HQ

5

２．Course summary
２－１ Assembling participants
The actual training practice was conducted in Ubon Ratchathani because of its suitability offield sites, however the reception of participants, opening ceremony and keynote speech were
held in Bangkok.
２－２ Opening ceremony
After the registration, Dr. Chalermchai Boonyaleepun, president of Srinakharinwirot
University, Dr. Masaya Tatara (BIODIC, MoEJ), Dr. Filiberto Pollisco (ASEAN Centre for
Biodiversity) made opening remarks. Ms. Mari Takehara gave a presentation introducing
ESABII. A keynote speech “Taxonomy of freshwater fishes in Southeast Asia, its past, current
and future” was given by Professor Dr. Yasuhiko Taki, a special trainer of this course.

図２．シーナカリンウィロット大学長

Figure 1. President of Srinakharinwirot Univ.

Figure 2. Dr. Tatara makes opening remarks
図３．鑪氏（環境省）

図４．多紀特別講師基調講演

図５．歓迎パーティー

Figure 3. Keynote speech by Prof. Dr. Taki
２－３ Museum Visit
In the morning following the opening ceremony a study tour was conducted at the Natural
History Museum in National Science Museum in Phatum Thani. Participants visited thespecimen management unit of the museum, where there could be shown the importance ofand difficulties in keeping historical and/or type specimens. After having lunch in themuseum, we flew to the second and main venue of the training course: Ubon Ratchathani
University in the east of Thailand.

6

Figure 4. Specimen management unit of Natural History Museum
２－４ Lectures on freshwater and brackish water fish taxonomy
In the morning of the first day in Ubon Ratchathani, the following lectures were given.


Current status on freshwater and estuarine fishes in Southeast Asia
(by Dr. Chavalit Vidthayanon)
System of naming fishes—how to propose a new species(by Dr. Koichi Shibukawa)
Learning how to prepare and manage fish specimens
Guidance about practicals in the course(by Mr. Chaiwut Grudpan)

Dr. Vidthayanon began with an inspiring talk about both the known and unknown issuesof freshwater and estuarine fishes in Southeast Asia to date. He also pointed some problems
that arose in the region such as habitat degradation caused by dam construction,
deforestation, etc.
Dr. Shibukawa illustrated his presentation with a first hand account about a new species
that was described from the Mekong Delta through a project of Nagao Foundation. He
emphasized that before a new species can be confidently described, a thorough check of allavailable literature and specimens needs to be done before a species can be proven to be new
to science.
Mr. Grudpan spoke about basic principle on biodiversity study such as: how to preservefish as permanent specimens, what kind of data should be accompanied with them, etc.
Participants were also informed that the training course would be implemented inaccordance with that principle.

Figure 5. Lecture room

Figure 6. Dr. Vidthayanon giving a lecture7

２－５ Fish identification practice with prepared specimens
２－５－１ Laboratory works
After the lunch break, laboratory work began in a room of the Fisheries Department at theFaculty of Agriculture. The room had enough space to accommodate all the participants, andalready furnished with the necessary equipment such as desk lamps, specimen trays, forceps,
etc.

Figure 7. Laboratory work
２－５－２ Identification practice with prepared specimens
Typically, a novice looking at a fish tends to pay attention to more obvious features such ascolor, size and overall shape, however freshwater fish species are very similar in overall
appearance. Furthermore, color is usually faded or altered in preserved specimens preserve
the only way to distinguish the species is to examine less obvious details, such as the numberof fin rays, barbels and the arrangement of scales are often the preserved specimens.
The participants were also shown a staining method using cyanine blue, a dye fortemporarily staining fish specimens to make their less obvious features more visible. In thisperiod, participants successfully mastered terminologies on fish body parts, and methods ofcounting and measurements.

Figure 8. Participants observing prepared specimens8

２－６ Sampling fish in the field (large river) and photographing fish specimens
２－６－１ Capturing fish and treatment of fish specimens in the fieldOn the first day of field practice, participants helped some local fishermen harvest their fishby means of large seine net in a large, slow-flowing river, and selected a proportion of the fish
landed for later identification. Some captured fish were kept alive in aerated containers.
Those fish that died during the sampling operation were immediately put in ice-cold waterand sent to the laboratory.

Figure 9. Seining for fish specimens in the main stream of the Mun River

Figure 10. Fish landing

Figure 11. Ice-cold fresh specimens

9２－６－２ Sorting and fin-setting of specimens
Fresh specimens brought back from the field were numbered and sorted by species (Figure12). As is often the case with other animal specimens, fish specimens are fixed by dipping in
10% formalin, but if a fish specimen is fixed in a state with its fins retracted, it is difficult tocount the fin rays therefore its fins need to be manually extended and fixed prior to the full
fixation of body (Figure 13). In order to fix the extended fin tips and barbels as they would bein the water, whole body of each specimen is dipped in formalin solution, and their fins andbarbells are expanded with insect pins (Figure 14). During this process, participants wererequired to wear a mask and to keep the laboratory ventilated well (Figure 15). Covering
exposed parts of specimens with a piece of tissue paper soaked in formalin helps avoid any
color gap between the two parts. Duration of fixation for fins and barbels should be less thanabout 15 minutes because keeping in formalin longer time fades natural color of the fish.

Figure 12. Sorting by species

Figure 13. Fin-setting in the air

Figure 14. Fin-setting in fixative liquid

Figure 15. Fin-setting work10

２－６－３ Photographing specimens
Fin-set specimens are then transferred into clean fresh water and put in a case forphotographing. Photographing a specimen submerged in water is preferable as the fish not
only looks more lifelike, but also to reduce reflections from the wet and shiny parts of the fish.
Some fish tend to float, and need to have the air extracted from the body cavity with a syringein order for them to submerge properly (Figure 16).
For photographing scientific specimens, traditionally a digital single lens reflection camera(DSLR camera) is used (Figure 17) but these days most modern digital cameras can be used.
Many participants took pictures of their specimens with their own cameras (Figure 18).
After photographing, specimens are well-fixed in 10% formalin for about 3 weeks, andtransferred into 70% ethyl alcohol for long term preservation.

Figure 16. Positioning a sample in a photographing case

Figure 17. SLR camera

Figure 18. Compact camera
11２－７ Sampling fish in the field (fast-flowing river), species identification and preparationof presentation
２－７－１ Capturing fish and treatment of specimens in the fieldOn the second day of field practice, field sampling was conducted in the Upper Mun River,and a rocky area with rapids and fast flowing water (Figure 19). A lot of unique species thatare well-adapted to this habitat were collected (Figure 20). Many of rapid water fishes arequickly moving fishes thus it is difficult to capture them with only scoop nets. Others
adhere to and/or hide under rocks in rapid water are also not easy species to capture withconventional methods, therefore we used an electric shocker after obtaining permission
from local authorities. Use of electric shocker is usually forbidden but is recommendable forresearch purpose because it is selective and only targets fish between the negative andpositive electrodes. Fish stunned by electric shock are easily scooped by hand nets (Figure21). In many cases, the stunned fish recovers and starts swimming again within a few
minutes. Care must be taken not to electrocute any people watching. Treatment of collectedfish samples is the same as mentioned in the previous section.

Figure 19. Habitat of rocky rapid

Figure 20. Fishes of rocky rapid

Figure 21. Fish collection with an electric shocker

12２－７－２ Species identification
Fish collected during the two days of field collection were identified to species. The
reference book “Fishes of Cambodian Mekong” written by Rainboth (1996) was used for
identification. Although the book was published about 15 years ago and information isalready out of date, the identification key was still useful for this training.

Figure 22. Species identification
２－７－３ Preparation of presentation
Species identification is not just a quiz for fish name, but a process of checking charactersthat leads to species. Participants were divided into six groups and representative of eachgroup would give a presentation in the next day. Each group busily prepared the slides
together with trainers in charge of each group till late evening.

Figure 23. Preparation of presentation13

２－８ Presentation of the results
Presentation of results was given by each of the six groups (Figure 24). Each group correctly
determined the diagnostic characters of freshwater and brackish water fishes that they
identified. Their slides contained a lot of pictures that they took by themselves (Figure 25)
and all groups had successfully grasped the methodology of species identification andpresentation.

Figure 24. Presentation of results

Figure 25. Examples of slides from the presentations14

２－９ Awarding certificate
Just after the presentation, certificates were awarded to each participant from organizers.
Dr. Pollisco of ACB and Dr. Tatara of MoEJ gave concluding speeches.

Figure 26. Awarding certificate
Conclusion
This training course was conducted as part of an international endeavor aiming to conserve
all the species existing on the earth, and was designed to develop parataxonomists who areable to identify species precisely to a certain extent. We believe that the only way to achieve
the aim is to build capacity of people who:
 have established roots in their regions are willing and able to enter the field, in search of wildlife can identify species accurately can record the status of biodiversity correctly can conduct monitoring surveys of that biodiversity can provide advance warning of threats to that of biodiversity in the rapidly
changing global environment.
We do not want participants to be satisfied only with knowledge of taxonomy. As the course
planner, we honestly wish them to be the persons who: will collect primary data on biodiversity in their region
 who provide reliable information from their region
no matter what occupation they have.

Figure 27. People associated with the training course
15

Training Course on Freshwater/Brackish-water Fish Taxonomy
January 2013
ESABII Secretariat
Biodiversity Center of Japan, Nature Conservation Bureau,
Ministry of the Environment
5597-1, kenmarubi, kamiyoshida, Fujiyoshida, Yamanashi, 403-0005 JAPANTEL: 0555-72-6033

FAX: 0555-72-6035

Title: Taxonomic Capacity Building for Global Biodiversity Monitoring in thefiscal year of 2012
Contractor: Japan Wildlife Research Center
3-10-10 Shitaya, Taitou-ku, Tokyo 110-8676 JAPANTEL: 03-5824-0960
FAX: 03-5824-0961

Nagao Natural Environment Foundation
3-10-10 Shitaya, Taitou-ku, Tokyo 110-0004 JAPANTEL: 03-5824-0771
FAX: 03-5824-0772