S011E

High Quality Peptide Cosmeceutical Ingredients
HiPep Laboratories have been promoting R & D aiming at industrial application of molecular recognition
mechanism in organisms. The main product objective is diagnosis and novel therapeutic drugs using peptides
and proteins as target compounds. Based on the abundant knowledge and experience of Peptide Science, wedeveloped functional peptides as cosmeceutical ingredients [from research to industrial level]. Developed
functional peptides are shown below.

① Antioxidant, Antiaging ② Wrinkle Release ③ Whitening ④ Scar & Keloid Release⑤ Hair growth ⑥ Regeneration of hair root cells
It is also possible to combine these functional peptides as cosmeceutical ingredients (differentiation from otherproducts). Furthermore, bioconjugates of these peptides, it is also possible to additional functions such assolubility, antibacterial activity. No patent has been issued for many products. We will also analyze counter ionsby making full use of up to 100 g; Price depends on amounts and structures; Delivery term 1 – 2 Months.
know-how for pharmaceuticals, especially in certification of purified products. In addition, we offer trifluoroacetic
acid-free purified products, which is often a problem with synthetic peptides. Supply minimum 10 g; One batch
up to 100 g.
Examples of peptides analyzed structure
at HiPep Laboratories and bulk
production: Functional peptides already
added to commodities in Europe and theUnited States, no structural disclosure,usually no patent application.

Wrinkle Release
PeptideWhitening
PeptideExamples of commissioned research
HiPep Laboratories supply functional peptide for cosmeceutical ingredients and activity evaluation on client sidedevelops new cosmeceutical ingredients product such as antioxidant peptides, tyrosinase inhibitors, unnaturalamino acid Mimosine (plant derived), regenerative medicine: angiogenic peptide, regeneration of hair root
cells (distinguished from hair growth). In the case of collaborative research we would like to have a client withan assay system. Concerning the structure, we will sign a Non-disclosure agreement (NDA). Structure
disclosure of peptides already known as functional peptides is performed under separate NDA.

HiPep Laboratories have the high technology of immobilization peptide. We utilize thistechnology to sell micro beads for manufacturing and biochips for peptides.

Peptide for functional
cosmetic validation【Sequence】

Ac-RFAACAA-COOH
Ac-RFAAKAA-COOH

Purity >95％

DPRA: The Direct Peptide Reactivity Assay (DPRA) is an in chemico method used to predict epidermal protein
binding. Binding of epidermal proteins is the molecular initiating event on the Adverse Outcome Pathway. The
DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test
chemicals. We manufacture and sell synthetic Cys and Lys peptides as the test material.
Advantage of DPRA: Compared with local lymph node proliferation test (LLNA) or guinea pig maximization
method (GPMT) using mouse, it can be carried out inexpensively in a short period of time.
Proposal for simplification of assay by immobilization: Although traditional solution method requires
reversed phase HPLC, assay method by immobilization does not require, so it is considered that simple andlow cost assay is possible. In particular, the resin used for synthesis of immobilized peptides is a support
obtained by graft copolymerizing polyethylene glycol (PEG) with polystyrene. This support, named TentaGel®,behaves in the same way as in organic solvents even in aqueous systems because the reaction site iscompletely in the solvent by the PEG spacer, and it is very excellent in swelling characteristics.
The resin is relatively stable biologicallyand chemically. However, ultrasonicwave and mechanical stirring can not
be performed. Peptide stability depends
on the peptide bound. It is usuallyusable from pH 2.2 to near neutral. Useof detergent is not problems.

Immobilized peptide DPRA-TentaGel SNH2HiPep Laboratories Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto, 602-8158, Japan
TEL: 075-813-2101, FAX: 075-801-0280, E-mail: info@hipep.jp, URL: http://www.hipep.jp/