09

Normal-Phase Columns

YMC-Pack SIL, YMC-Pack SIL-06 ------------------------ 96
YMC-Pack Diol-NP --------------------------------------- 96
YMC-Pack CN -------------------------------------------- 97
YMC-Pack PVA-Sil --------------------------------------- 97
YMC-Pack Polyamine Ⅱ ------------------------------- 98-99
YMC-Pack NH2 ------------------------------------------ 100
YMC-Pack PA-G ----------------------------------------- 100
Ordering Information------------------------------------ 101

09

Normal-Phase Columns
Analytical columns

YMC-Pack SIL, YMC-Pack SIL-06
High quality spherical porous silica gel
Normal-phase separation due to surface silanol groupsTwo different pore sizes are available
Useful for separation of compounds with similar structures

Pore size : 60, 120 ÅUsable pH range : 2-7.5
USP L3

Standard normal-phase column
YMC-Pack SIL is suitable for separation of fat-soluble compounds using non-polar mobile phase and separation of positional isomers
that are difficult to separate in reversed-phase mode. SIL-06 (pore size 60 Å) has greater adsorption properties than SIL (pore size120 Å) due to its larger specific surface area, and is generally useful for separating natural products with a low molecular weight.

Application
1(G910523S)

Tocopherol isomers

3 4

1. α-Tocopherol
2. β-Tocopherol
3. γ-Tocopherol
4. σ-Tocopherol

2Column: YMC-Pack SIL (5 μm, 120 Å)
150 X 4.6 mmI.D.
Eluent: n-hexane/THF/acetic acid (97/3/0.25)
Flow rate: 1.0 mL/min
Temperature : 30°CDetection: UV at 295 nm

010minNormal-Phase Columns

Analytical columns

YMC-Pack Diol-NP
Pore size : 60, 120 ÅUsable pH range : 2-7.5
USP L20

Silica gel bonded with dihydroxypropyl groups
Normal-phase separation using non-polar solvents
Useful for hydrophilic interaction chromatography (HILIC)Different separation characteristics from bare silica gel

Different separation characteristics from bare silica gel
YMC-Pack Diol-NP shows retention behavior of normal-phase chromatography when it is used with low-polarity mobile phases.
Hydroxyl groups on the surface of the stationary phase act as polar groups. YMC-Pack Diol-NP is as widely applicable to normalphase separation as silica gel. It is also useful in cases where separation optimization is difficult to achieve using bare silica gel. Inaddition, it is available for HILIC mode separation by using organic/water mobile phases.

Separation characteristics of Diol-NP and SIL
YMC-Pack Diol-NP

YMC-Pack SIL
61.

64113435566455222.

396

0

55 10（A010526A）（A010526A）

610 15

15 20

331

1min

OH

OH

OH
HO

4.

ColumnOH

5Hydr oquinone

Pyrogallol
Pyrogallol

OH

HOHO

HO OH

OHOH

6.
6.
OH
OH OH
OH

Resorcinol
Resorcinol

05.

HO

5.

HOOH

5 10（A010526C）（A010526C）

10 15 min15 minOHOH

HOPhlor oglucinol
Phlor oglucinol

Catechol

3.

0OHHO

3.
OHHO

220
min

4.
OH

PhenolOHHOOH OH

2.

10

5.

Catechol
Catechol

252.
OH

3.

34

OH

Hydr oquinone
Hydr oquinone

452

4.

1.

OH

PhenolPhenol

42

1.
OH

6

Pyrogallol

6.

OH

HO

HO

Resorcinol

Phlor oglucinolOH: 5 μm, 120 Å
250 X 4.6 mmI.D.
Eluent: n-hexane/ethanol (80/20)
Flow rate: 1.0 mL/min
Temperature : 30°C
Detection: UV at 254 nm
Differences in separation characteristics ofDiol-NP and SIL for separating phenols areshown on the left. Diol-NP shows longer
retention and better separation than SIL in
separating these compounds.

Analytical columns

YMC-Pack CN
Pore size : 120 ÅUsable pH range : 2-7.5
USP L10

Silica gel chemically bonded with cyanopropyl groups
For both normal-phase and reversed-phase modesDifferent separation characteristics from bare silica gel
Faster column equilibration than bare silica gel

Column can be used in both normal-phase and reversed-phase modes
YMC-Pack CN shows retention behavior of normal-phase chromatography when it is used with low-polarity mobile phases such as
hexane. Since YMC-Pack CN stationary phase surface is less polar than bare silica gel, the retention times of analytes are generally
shorter than with bare silica gel. YMC-Pack CN is therefore appropriate for samples having too strong retention when analyzed using
bare silica gel. In contrast, YMC-Pack CN shows retention behavior of reversed-phase chromatography when it is used with highpolarity mobile phase, such as methanol and water. Although separation modes are selectable according to the purpose of separation,
it is preferable to use one column dedicated for one separation mode in consideration of the life of the column.

Application(A010619A)

Nitroaniline isomers

211.

2.
CH3345

3.
NO2Toluene

Nitr obenzene

4.
NH25.

NH2

NH2

NO2

o-Nitroaniline

NO2
m-Nitroaniline

NO2
p-Nitroaniline

Column1020 minAnalytical columns

YMC-Pack PVA-Sil
Vinyl alcohol polymerised silica
High stability and reproducibility

Pore size : 120 Å
Usable pH range : 2-9.5(Recommended pH range : 2-7.5)
USP L24

Normal-Phase Columns

0: YMC-Pack CN (5 μm, 120 Å)
250 X 4.6 mmI.D.
Eluent: n-hexane/2-propanol (80/20)
Flow rate: 1.0 mL/min
Temperature : 30°CDetection: UV at 254 nm

Polyvinyl alcohol functionalized silica
YMC-Pack PVA-Sil, bonded with a monomolecular polymer coating of vinyl alcohol (PVA), completely covers both external andinternal surfaces of the silica support, protecting it against aggressive, high pH buffers and solvents. The PVA polymer shell on PVASil deactivates the silica support while providing a hydrophilic surface.

Application
Potato LipidsSASG

PC

ColumnEluent

PE

DG

MGDG

DGDG

CER
SG

SE?

08?

16PG

24Time (min)PI3240: YMC-Pack PVA-Sil (5 μm, 120 Å), 250 X 4.6 mmI.D.
: A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCI3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Flow rate: 1 to 2 mL/min
Gradient: Tmin : 0 5 15 40 40.1 45 50
B% : 0 20 52 52 70 0 0
C% : 0 0 4 14 0 0 0
Flow (mL/min) : 1 1 1 1.4 1.4 2 2Nebuliser temperature : 25°C, Evaporation temperature : 35°C
Detector : ELSDSample
S : Sterols		
CER : Cerebrosides
SE : Sterol Esters
SG : Steryl glycosides
MGDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine
PG : Phosphatidyl glycerols
PC : Phosphatidylcholine
ASG : Acylsterylglycosides
PI : Phosphatidylinositol
DG : Diacylglycerol
Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol.18 (1995) 97-1009709

Normal-Phase Columns
Analytical columnsYMC-Pack Polyamine Ⅱ
Pore size : 120 ÅUsable pH range : 2-7.5
USP L111

Silica gel chemically bonded with polyamine
The most suitable column for separation of sugars
Useful for separation of hydrophilic compounds including vitamins
Useful for separation of fat-soluble compounds using nonaqueous mobile phase
Higher durability than conventional silica-based amino columns

Amino column with improved durability
YMC-Pack Polyamine Ⅱ is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column
lifetime of YMC-Pack Polyamine Ⅱ in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable
to separation of oligosaccharides using mobile phase with relatively higher water content. In addition, YMC-Pack Polyamine Ⅱ can be used
to separate ionic compounds with a combination of normal-phase mode and weak anion exchange mode.

Retention
Retention
time (min)time (min)Excellent durability30

Lactose30

LactoseMaltose20

MaltoseSucrose20

Sucrose<Flow conditions>
Column:Y
 MC-Pack Polyamine Ⅱ250 X 4.6 mmI.D.
Eluent:w ater
Flow rate:1
 .0 mL/min
Temperature : ambient
Time:1
 00 hours<Analytical conditions>
Eluent:a cetonitrile/water (75/25)
Flow rate:1
 .0 mL/min
Temperature : 26°CDetection:R I, 32 X 10-6 RIU/FS

GlucoseNormal-Phase Columns

10

Fructose
Glucose10

Fructose

0050

Even in a hard flow durability test using a mobile phase that consists onlyof water, the retention time of sugars changes little.

100

Continuous flow50
of water (time, hr)100
Continuous flow of water (time, hr)

The most suitable columns for separation of sugars, including oligosaccharides
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
5. Lactose

1. Maltoundecaose (G11)
2. Maltododecaose (G12)
3. Maltotridecaose (G13)
4. Maltotetradecaose (G14)
5. Maltopentadecaose (G15)

1 211 2 33145452233454
5040

min

10203040: YMC-Pack Polyamine Ⅱ250 X 4.6 mmI.D.
Eluent: acetonitrile/water (55/45)
Flow rate: 1.0 mL/min
Temperature : 26°C
Detection: RI, 32 X 10-6 RIU/FS

min

0Column98

102030(T910718A)

0102030min

102030 min:Y MC-Pack Polyamine Ⅱ250 X 4.6 mmI.D.
Eluent:a cetonitrile/water (75/25)
Flow rate:1
 .0 mL/min
Temperature : 25°C
Detection:R I, 32 X 10-6 RIU/FS0Column(F030618H)

Elution volume of sugars and sugar alcohols

For normal-phase separation
165

Tocopherol isomers
1.

Lactitol
Palatinit

60

CH 3

HO
H3 CO
CH 3

2Lactose

2.

α-Tocopherol

CH 3HO55

MaltoseOCH 33.

3β-Tocopherol

HO50

H3 CMaltitolRaffinose

Elution volume (mL)HOO

Palatinose

CH 3: YMC-Pack Polyamine Ⅱ
250 X 4.6 mmI.D.
Eluent: n-hexane/ethly acetate(70/30)
Flow rate: 1.0 mL/min
Temperature : 30°C
Detection: UV at 295 nm

GlucoseMannitol

35

Sorbitol30

Galactose*

25

20

Mannose*01030 min （T920728E）

1. Nicotinic acid
2. Erythorbic acid
3. L-Ascorbic acidArabitol
Ribitol
Xylitol
23

XyloseGlycerol: YMC-Pack Polyamine Ⅱ
250 X 4.6 mmI.D.
Eluent: acetonitrile/50 mM NH4H2PO4 (70/30)
Flow rate: 1.0 mL/min
Temperature : 30°CDetection: UV at 250 nm
ColumnSucralose

501020min（A970603A）

075%

80%

85%

Acetonitrile concentration (%)*Not detected with 85% acetonitrile

Normal-Phase Columns

Rhamnose

1020

Water-soluble vitamins

1Frucutose

FucoseErythritol

15

σ-Tocopherol

ColumnSucrose40

γ-Tocopherol

4.

Inositol

45O
CH 34

YMC-Pack PolyamineⅡ is applicable for separation of fat-soluble vitamins
and water-soluble vitamins as a normal-phase column that can be usedwith water or buffer and various organic solvents.

99

09

Normal-Phase Columns
Analytical columns

YMC-Pack NH2
Silica gel chemically bonded with aminopropyl groups
Useful for separation of sugars
Enables normal-phase mode separation using aqueous or nonaqueous mobile phasePore size : 120 ÅUsable pH range : 2-7.5
USP L8

Normal-phase separation column utilizing amino groups
YMC-Pack NH2 is a normal-phase separation column utilizing the polarity of primary amino groups. It is also applicable to separations
utilizing weak anion exchange. YMC-Pack NH2 is often used for separation of sugars.

Application(T920525D)

Nucleotides

2

1. 5’-CMP
2. 5’-AMP
3. 5’-UMP
4. 5’-IMP
5. 5’-GMP

31Column: YMC-Pack NH2 (5 μm, 120 Å)
250 X 4.6 mmI.D.
Eluent: 50 mM KH2PO4-H3PO4 (pH 3.5)
Flow rate: 1.0 mL/min
Temperature : 40°C
Detection: UV at 260 nm

45Normal-Phase Columns

010minAnalytical columns

YMC-Pack PA-G
Silica gel chemically bonded with polyamine
Useful for separation of acidic oligosaccharides

Pore size : 120 ÅUsable pH range : 4-7.5

Normal-phase separation column utilizing amino groups
YMC-Pack PA-G is useful for separation of acidic oligosaccharides. YMC-Pack PA-G is similar selectivity as YMC-Pack PA.

100

Ordering Information –Columns–
YMC-Pack SIL
Column length (mm)Phase Column I.D.
dimension
(mm)
120 Å
3 μm
120 Å
5 μm

4.6
6.0
4.6
6.0
10

50
—
—
—
—
—

75
—
——
—
—

50
—
—
—

75
—
—
—

100
SL12S03-1046WT
SL12S03-1006WT
SL12S05-1046WT
SL12S05-1006WT—Guard cartridges

150250SL12S03-1546WT—SL12S03-1506WT—
SL12S05-1546WT SL12S05-2546WTSL12S05-1506WT SL12S05-2506WTSL12S05-1510WT SL12S05-2510WTI.D. (mm)

10 mm length

4.0

SL12S03-0104GC

4.0

SL12S05-0104GC

10SL12S05-0110CC

YMC-Pack SIL-06
Column length (mm)Phase Column I.D.
dimension
(mm)
60 Å
5 μm

4.6
6.0
10Guard cartridges

100150250
SL06S05-1046WT SL06S05-1546WT SL06S05-2546WT
SL06S05-1006WT SL06S05-1506WT SL06S05-2506WT—
SL06S05-1510WT SL06S05-2510WTI.D. (mm)

10 mm length4.0

SL06S05-0104GC

10SL06S05-0110CC

YMC-Pack Diol-NP *Shipping solvent for Diol-NP is n-hexane/2-propanol (99.5/0.5). In case of using eluent including water, take care of miscibility.
Column length (mm)Phase Column I.D.
dimension(mm)

Guard cartridges

5075

100

150250I.D. (mm)

10 mm length

Diol-6060 Å
5 μm

3.0—

——

DN06S05-1503WT—

3.0

DN06S05-0103GC

4.6

—

—

DN06S05-1046WT DN06S05-1546WT DN06S05-2546WT4.0

DN06S05-0104GC

Diol-120120 Å
5 μm

2.03.04.6——
DN12S05-0546WT————
DN12S05-1502WT——DN12S05-1503WT—
DN12S05-1046WT DN12S05-1546WT DN12S05-2546WT

2.1
3.0
4.0

DN12S05-01Q1GC
DN12S05-0103GC
DN12S05-0104GC

YMC-Pack CN *Shipping solvent for CN is acetonitrile/water (50/50). Take care of miscibility in normal-phase separation.
Phase Column I.D.
dimension
(mm)

120 Å
5 μm

300 Å
5 μm

Guard cartridges
I.D. (mm)
2.1
3.0
4.0
2.1

10 mm length
CN12S03-01Q1GC
CN12S03-0103GC
CN12S03-0104GC
CN12S05-01Q1GC

4.0

CN12S05-0104GC

102.1

CN12S05-0110CC
CN30S05-01Q1GC

4.0

CN30S05-0104GC

YMC-Pack PVA-Sil
Column length (mm)Phase Column I.D.
dimension
(mm)
120 Å
5 μm

4.6

5075

PV12S05-0546WT—

100

Normal-Phase Columns

120 Å
3 μm

2.0
3.0
4.6
2.0
4.6
6.0
102.0
4.6
6.0

Column length (mm)5075
100150250
CN12S03-0502WT CN12S03-L502WT CN12S03-1002WT CN12S03-1502WT—
CN12S03-0503WT—
CN12S03-1003WT CN12S03-1503WT———
CN12S03-1046WT CN12S03-1546WT————
CN12S05-1502WT CN12S05-2502WT—
CN12S05-L546WT CN12S05-1046WT CN12S05-1546WT CN12S05-2546WT——
CN12S05-1006WT CN12S05-1506WT CN12S05-2506WT———
CN12S05-1510WT CN12S05-2510WT———
CN30S05-1502WT CN30S05-2502WT—
CN30S05-L546WT CN30S05-1046WT CN30S05-1546WT CN30S05-2546WT——
CN30S05-1006WT CN30S05-1506WT CN30S05-2506WT

Guard cartridges
150250PV12S05-1046WT PV12S05-1546WT PV12S05-2546WT

I.D. (mm)

10 mm length

4.0

PV12S05-0104GC

YMC-Pack Polyamine Ⅱ
Phase Column I.D.
dimension
(mm)
120 Å
5 μm

4.66.010Column length (mm)
50—
——

75—
——

100
—
—
—

Guard cartridges

150
250
PB12S05-1546WT PB12S05-2546WTPB12S05-1506WT PB12S05-2506WT—PB12S05-2510WT

I.D. (mm)

10 mm length

4.0

PB12S05-0104GC

10PB12S05-0110CC

YMC-Pack NH2
Phase Column I.D.
dimension
(mm)
120 Å
5 μm

4.66.010Column length (mm)
50
—
——75
—
——

50

75

100

—

—

—

Guard cartridges

100
150
250
NH12S05-1046WT NH12S05-1546WT NH12S05-2546WT
NH12S05-1006WT NH12S05-1506WT NH12S05-2506WT—NH12S05-1510WT NH12S05-2510WT

I.D. (mm)

10 mm length

4.0

NH12S05-0104GC

10

NH12S05-0110CC

YMC-Pack PA-G
Phase Column I.D.
dimension
(mm)
120 Å
5 μm

4.6

Column length (mm)Guard cartridges
150250PG12S05-1546WT PG12S05-2546WT

I.D. (mm)

10 mm length

4.0

PG12S05-0104GC* Guard cartridge holder required, part no. XPGCH-Q1 for 2.1 - 4.0 mmI.D. and XPCHSPW1 for 10 mmI.D.
＊ See pp.115-116 for preparative columns other than those listed above.
101