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Over view of HiPep Products and Services

PepTenChip®, a novel biodetection system

Mimosine (Mim), nonproteinogenic amino acid,and mimosine containing
peptidesVarious peptide-based
libraries for basic researchand drug discovery: acyclotide immobilized on a
gel-type bead/peptidevehicles for DDS

Research tools
Labeled peptides arrayed as capture molecules, amorphous
carbon plates, and detection device (on-site use &maintenance free), in which basic technologies have been
completed.
Mim has several biological functions. Large scale productionof high quality Mim has been performed and antioxidative
peptides have been developed.
Application of mimosine containing peptides as ingredientsfor drugs/functional cosmetics: collaboration and /ortechnology transfer to companies having markets.
Libraries/Bioconjugates are useful tool in R and D, moreover
drug discovery and development such as optimization.
Discovery and development are also carrying out as own
research projects. The technologies with materials can betransferred.

Visualization of human telomere has been successfullyperformed. Telomere should be one of the importantbiomarker in examining the aging process and tumorigenesissince the length of telomere is believed to be associated withaging or cancer diseases.
Early stage of preclinical
Small peptides exhibiting strong angiogenesis has beenfound in animal experiments and SAR has been elucidated.
Angiogenic peptides andtheir conjugates forLicensing and clinical applications by the collaboration ofreconstruction medicine
angiogenic peptides (patented in USA & Japan) arerequested
Synthetic peptide based on design, peptides/polyamidesconsisting of non-proteinogenic amino acids, Nmethylpyrrole and N-methylimidazole as major buildingblocks, designated PIPAs, bind to specific nucleotide
sequences in the minor groove of double helical DNA with
DNA-recognition by PIPAs(Peptide-DNA interactions)high affinity and specificity. PIPA has functions for gene
regulation through DNA binding and applied as novel
chemical probes or vehicle/shuttles for delivery that is theuse for diagnostics and/or therapeutic agents. PIPLS (Large
Scale Synthesis System) has been constructed.
Research Support Business: Contract syntheses & analyses
Devices/consumers & novel
HPLC-columns with highSeveral devices focusing on highly efficient libraryresolution developed for in- construction in addition to technology know-how can beapplied contract based works.
house use and contract
researches
Novel telomere staining
probe (HPTH59, afluorescent dye labeledPIPA)HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto, 602-8158, Japan
Tel +81-75-813-2101 E-mail: PepTenChip@hipep.jp URL: http://www.hipep.jp/

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Contract based Research, Syntheses and Characterization
Carried out by using in house developed materials/devices
Libraries: Design, Synthesis and Construction of Peptide/ Polyamide derivatives, and Bioconjugates: Design,syntheses and characterizations: High quality peptides containing not only natural amino acids but also nonproteinogenic amino acids, Glycopeptides, Lipopeptides, Cyclic peptides, Phosphopeptides, Sulfated peptides,
Glutathione derivatives (metabolites), Pyrrole-imidazole polyamides, Peptide Nucleic Acids, Protein syntheses
by chemical ligation, Cell penetrating peptides, Peptide-Vehicles for DDS
Analyses: Amino acid composition, Determination of residual organic solvents/acids, water contents, Chiralanalyses = GMP-GLP approved
Characterization: LCMS, MS/MS, MALDI-TOF-MS/MS, Protein-sequencing by the Edman degradation
Microarray printing services: Utilizing the most advanced facilities and expertise on patented concepts withnovel substrates made from amorphous carbon and/or high quality glass substrate slides (operated in the class
100 clean room)
Biochip detection service: Fluorescent scanner or a fluorescent microscopy, further MALDI-TOF-MS①Production of starting resin, by the in-house developed
PetiSyzer®

② Automated Synthesizer PSSM-8, by Nokihara in 1991
(operated over night)

Peptide Construction Libraries

Screening + AnalysesSequencing③ Manual syntheses for costly reagents, difficult sequences④ Cleavage by PetiSyzer®

Tailor made Bio-detection, Biochip-fabrication
Analytes⑤ Large scle purification by in-house packed columns

Capture Mol. immobilized
SPPS
by 1～2 LiterReactors⑥ Use of multiple LC-MS & MALDI-TOF/TOF
Clean Room (class 100)

Arraying & detection services
Chip&MS

HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto, 602-8158, Japan
Tel +81-75-813-2101 E-mail: PepTenChip@hipep.jp URL: http://www.hipep.jp/