Instructions For Use

HBK-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 3

Page 1 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Orcein Stain Kit(For Hepatitis B and Elastic Fibers)
Description:

The Orcein Stain may be used in histology procedures for the visualization of Hepatitis B surface
Antigen (HBsAg), elastic fibers, and copper deposits. HBsAg appears as irregular shaped aggregates inthe cytoplasmic region of the cells. This reagent may be used on formalin-fixed, paraffin-embedded
sections.
HBsAg:
Elastic Fibers:
Copper Assoc. Proteins:
Background:

Dark Red/Brown
Dark Red/Brown
Dark Red/Brown
Light Reddish/Purple

Uses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

Known hepatitis positive liver.
Lung for elastic fiber.
Any well fixed tissue cut 3-5 microns.

Ordering information regarding additional recommended
individual components on back page!
Availability/Contents:
Item #
PPG030
SAQ030OQB125OAA125
DSA125

Precautions:

Kit Contents
VolumePotassium Permanganate Sol. (5%) 30 ml
Sulfuric Acid Solution (3%)
30 ml
Oxalic Acid Solution (2%)
125 mlOrcein Solution
125 mlDifferentiating Solution
125 mlAvoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.

25° C
Storage: 18° C

Doc: IFU-Template18-25rev2

Storage18-25°C
18-25°C
18-25°C
18-25°C
18-25°C

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Instructions For Use

HBK-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 3Page 2 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Procedure:Prepare Oxidizing Immediately Prior to Beginning Procedure:
Combine:

50 ml
5 ml
3 ml

Distilled Water
Potassium Permanganate Solution (5%)
Sulfuric Acid Solution (3%)

Mix thoroughly.

1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

Incubate slide in freshly prepared Oxidizing Solution for 10 minutes.

3.

Rinse slide briefly in running tap water followed by 1 dip in distilled water.

4.

Incubate slide in Oxalic Acid Solution (2%) for 10 minutes or until clear.

Note: Section should be colorless following this step.
5.

Rinse slide for 1 minute in running tap water followed by 2 dips in distilled water.

6.

Incubate slide in coplin jar containing Orcein solution for 4-8 hours (2 hours is sufficient for elastin). Note: Ensure tissue isfully immersed in staining jar. Close lid to prevent evaporation.

7.

Rinse slide in Alcohol, Reagent (70%).

8.

Differentiate in Differentiating Solution for 10-60 seconds.

9.

Dip slide in Alcohol, Reagent (70%) and check slide microscopically for proper differentiation.

Note: Repeat step 8 if necessary.
10. Dehydrate quickly in 3 changes of absolute alcohol.
11. Clear, and mount in synthetic resin.
Note: If darker staining is preferred:1) Incubation time in Orcein solution may be increased.
and/or2) Differentiation may be omitted by replacing steps 7-9 with a simple rinse with deionized water.
References:1.
2.

Deodhar K.P., Tapp E., Scheuer P.J. Orcein staining of Hepatitis B Antigen in paraffin sections of Liver Biopsies. Journal of Clinical Pathology;
vol. 28: pages 66-70, 1975.
Salaspuro, M., Sipponen, P. Demonstration of an intracellular copper-binding protein by Orcein staining in long-standing cholestatic liverdiseases. Gut, 1976, volume 17: pages 787-790.

25° C
Storage: 18° C

Doc: IFU-Template18-25rev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Instructions For Use

HBK-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 3

Page 3 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Potassium Permanganate
Solution (5%)

PPG030
PPG12530 ml
125 mlSulfuric Acid Solution (3%)

SAQ125
SAQ500
SAQ999125 ml500 ml1000 ml

Oxalic Acid Solution (2%)

OQB500OQB999500 ml1000 ml

Orcein Solution

OAA125OAA500OAA999125 ml500 ml1000 ml

Differentiating Solution

DSA125
DSA500125 ml500 mlAlcohol, Reagent (70%)

EAS500
EAS999500 ml1000 ml

25° C
Storage: 18° C

Doc: IFU-Template18-25rev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands