YMC USER'S MANUAL

Column Care and Use Instructions

YMC-Pack Polyamine Ⅱ
YMC-Pack NH 2
YMC-Pack PA-G

1. Introduction
Thank you for purchasing a YMC high-performance liquid chromatography (HPLC) column. YMC-Pack Polyamine Ⅱ/ PA-G is asilica based polyamine column and YMC-Pack NH2 is a silica based aminopropyl column. Each product is suitable for separation ofmono- and oligosaccharides.
YMC HPLC columns, which are manufactured under highly controlled conditions, must pass a series of stringent tests before
being accepted for shipment. (Please refer to the column inspection report). To ensure optimal performance and durability of thecolumn, please read these instructions carefully before using this column.

2. Specifications
Usable temperature range
Packing material

Functional group

Usable pH range

Regular use(recommended)

Polyamine Ⅱpolyamine

NH2

aminopropylPA-G

polyamine

2 – 7.5

Upper limit
50 °C

25 – 35 °C

4 – 7.540 °C

3. Column connections
The “WT” or “PTH” at the end of the product code indicates the style of column endfittings.
WT = Waters style / PTH= Parker style

4. Shipping solventIndicated in the COLUMN INSPECTION REPORT. Replace with this solvent for storage. When replacing a mobile phase
containing buffer salts/additives, extra care must be taken to prevent salt precipitation.

5. Mobile phase
・ The correct direction of the solvent flow is indicated by an arrow on the column identification label.
・ Aqueous or non-aqueous solvent can be used as a mobile phase. Repetitive replacement among solvents with large differencein polarities might degrade the column performance. In general, acetonitrile, methanol, tetrahydrofuran (THF), and n-hexane arerecommended for regular use. When using THF or non-aqueous solvent as a mobile phase, be mindful of the solvent
resistance of your system or tubing (PEEK parts are especially unsuitable for use with THF).
・ Acetonitrile aqueous solution is a typical mobile phase for the analysis of saccharides.
・ The mobile phase containing buffer salts/additives can be used for separation of ionic compounds. After using these mobilephase, the same column is not recommended for saccharide analysis using acetonitrile/water, because it may change theretention characteristics and peak shapes.
・ Recommendations of pH and temperature for column use are shown in the specifications table in section 2. Column lifetime
varies depending on conditions of use such as pH, temperature, and mobile phase composition. In general, high temperatureand concentration of buffer or additive, low organic solvent concentration will shorten column lifetime.
・ When using the column at pH near the upper or lower limit, the column lifetime will shorten under certain conditions bytemperature and mobile phase composition.
・ When replacing the mobile phase between aqueous and non-aqueous solvent, flush the column with more than 10 column
volumes of 2-propanol etc. For example, acetonitrile/water (75/25) → 2-propanol → n-hexane/2-propanol (90/10).

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YMC USER'S MANUAL

6. Column cleaning (general method)・ When mixture of acetonitrile/water is used as a mobile phase for saccharides analysis, wash the column with a solvent
containing a higher ratio of water than the mobile phase and replace with the shipping solvent for storage.
・ In the case that the adsorption of polar substances is estimated after the normal phase separation with a non-aqueous mobile
phase, flush the column with ethanol or 2-propanol. After cleaning, replace with n-hexane/2-propanol (90/10) for storage.
・ For YMC-Pack Polyamine Ⅱ, when change in peak shape or retention time of acidic analyses or reducing sugars is observed,
wash the column with acetonitrile/water/28 % ammonia solution (70/30/0.1).

7. Other environments
・ The operating pressure should be kept under 15 MPa (2175 psi) for 150 mm length column or less, under 20 MPa (2900 psi)for 250 mm length column, under 10 MPa (1450 psi) for 10 mm I.D. column or more.
・ To prevent exposure of the column to excessive pressure, the sample solution should be filtered through a 0.2 μm membraneor smaller to remove particulates. We recommend using a pre-column filter to prevent the column frit from being clogged withsamples.
・ Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life.
・ Adjust the flow rate appropriately because the pressure changes depending on the column length, temperature, types oforganic solvent etc.

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM230123HE) 2/2