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PepTenChip® : Novel Bio-detection System
PepTenChip® is a Biochip for the next generation
used for characterization of proteins
Significance① Biomolecular recognition is structures to structures② Proteins can be mimicked by peptides.
③ Peptides can be designed and synthesized.
④ Peptides can make 3D-structures.
⑤ The PepTenChip® system does not involve only detection ofspecific molecules (such as markers) in a 1:1 manner, butalso unknown and surrogate markers responsible todiseases can be clarified.
⑥ The detection principle is the differences in fluorescent intensity change of capture peptides caused by analytes.
⑦ The structural change of the analyte-peptide interaction is reflected in the fluorescent-intensity changes of thecapture molecules in a dose dependent manner (PAT: JP-US-EU).
⑧ PepTenChip®, a sensor device, discriminates 3D-structures of proteins⑨ The novel chip material made from amorphous carbon has been developed, which has significant advantages
over conventional glass slides (PAT & PAT.P).
⑩ The PepTenChip® plates are suitable also for array consisting of DNA, sugar, antibody, antigen and low
molecular compounds.
⑪ The basic chemical modification of our surface is through a carboxyl group and further derivatization to aminogroup, bromoacetyl group, succinimide ester, maleimide or biotin-streptavidin is easily achieved.
⑫ The protein can be identified through a data base by the protein fingerprint method (Informatics).

HiPep Laboratories

Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto, 602-8158, Japan
Tel +81-75-813-2101 , E-mail: info@hipep.jp, URL: http://www.hipep.jp/1

CP02E

PepTenChip® : Novel Bio-detection SystemPepTenChip® System• In conventional methods analytes should be labeled, although in the PepTenChip ®
System capture molecules are labeled. After application of samples washing is notnecessary.
• Unlike proteins, peptides can be used after dried. One of advantages in industrial
production and higher throughput, saving costs, easier delivery. Mol. BioSys., 2,113, 2006
• Arrayed capture molecules of PepTenChip® are designed peptides, which had beensynthesized, purified and characterized. Additionally recognition can be structurally
optimized.
• Chip material: The novel material made from amorphous carbon has beendeveloped for biochips, which has significant advantages over conventional glass
slides as summarized in the next table. (PAT & PAT.P)
 No self-fluorescence Chemically inert
 Higher thermal & electric conductivity
 It can be used as a sample tray of MALDI-TOF-MS
 Environmentally friendly (regeneration is easy)
 Grinding Flatness (<10 micron) Extremely low back ground

Chip & MS
Dual Detection

 Lowest non-specific adsorption

Novel chip materials：Amorphous Carbon andsurface technology (PAT.),
Quality control(PAT.) Mechanically stable (easy handling) Higher stability (storage/transport)
 Uniformed distribution of functional groups (higher reproducibility) Higher reproducibility + sensitivity for protein detection
 Many surface chemistry (derivatization with vaiety of functional groups Patented method for Quality control: determination of surface aminogroupsFluorescence Detector•

Portable, Maintenance free, Easy handling, Space saving•

Scientific CMOS camera•

Image capture software•

LED Excitation•

Filters can be selected by Fluorescent dyes•Suitable for Bio-Safety-Labs 3 or 4

Bio-molecular Recognition using Biochip→ Medical examination
Research tools, safety control for food, agricultural chemicals, environmental analyses, diagnosis, early
detection and prevention of disease, home medical care, on-site examination• Glycopeptide array showed a different response to the toxin protein. Depending on the type of sugar is different
reactivity of the protein. This technology may contribute to the safety inspection of food.
• In world-wide no technologies similar to PepTenChip® are envisaged. Material and surface chemistry are unique.
• Products: chips, related equipment, contract based biodetection and arrays• Related articles and technical data can be down loaded from HiPep website

HiPep Laboratories

Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto, 602-8158, Japan
Tel +81-75-813-2101 , E-mail: info@hipep.jp, URL: http://www.hipep.jp/