Instructions For Use

AYH-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 4

Page 1 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

H. Pylori Rapid Stain Kit
Description:

The H. Pylori Rapid Stain Kit is designed for demonstrating Helicobacter Pylori infected tissue. Kit may
be used on formalin fixed, paraffin-embedded tissue.
Helicobactor Pylori:
Mucin:
Background:

BlueYellow
Light Blue

Uses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

Helicobacter Pylori infected stomach.

Ordering information regarding individual components
on back page!
Kit Contents:
Item #
PAQ500SME125
ANY125
TQF006SHC008Precautions:

Kit Contents
Periodic Acid Solution
Sodium Metabisulfite Solution
Alcian Yellow Solution
Toluidine Blue Solution
Sodium Hydroxide Solution (3%)

Volume500 ml
125 ml125 ml6 ml x 28 ml

Storage
2-8°C
18-25°C
18-25°C18-25°C
18-25°C

Avoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.

Procedure:
1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

Incubate slide in Periodic Acid Solution for 10 minutes (Note: Do Not Reuse Periodic Acid Solution).

3.

Rinse thoroughly in distilled water.

4.

Incubate slide in Sodium Metabisulfite Solution for 5 minutes.

5.

Rinse thoroughly in distilled water.

6.

Incubate slide in Alcian Yellow Solution for 5-10 minutes.
25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Instructions For Use

AYH-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 4Page 2 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Prepare Toluidine Blue Working Solution (Immediately prior to use):
Distilled Water
Toluidine Blue Solution
Sodium Hydroxide Solution

50 ml
20 drops
3 drops7.

Rinse slide thoroughly in distilled water.

8.

Incubate slide in freshly prepared Toluidine Blue Working Solution for 3-5 minutes (Note: Do Not Reuse Toluidine Blue
Working Solution).

9.

Rinse thoroughly in distilled water.

10.

Dehydrate quickly in 2 changes of absolute alcohol.

11.

Clear, and mount in synthetic resin.

References:1.
2.

Leung, K., Gibbon, K.J. A Rapid Staining Method for Helicobacter Pylori in Gastric Biopsies, Journal of Histochemistry, Volume 19, Pages
131-132. 1996
Vartanian, R.K., Leung, J.K., Davis, J.E., Kim, Y.B., Owen, D.A. A novel Alcian yellow-toluidine blue (Leung) stain for Helicobacter species:
comparison with standard stains, a cost-effectiveness analysis, and supplemental utilities. Modern Pathology, January 1998, Volume 11(1):pages 72-78.

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Periodic Acid Solution

PAQ250
PAQ500
PAQ999250 ml500 ml1000 ml

Sodium Metabisulfite Solution (5%)

SME125SME500
SME999125 ml500 ml1000 ml

Alcian Yellow Solution

ANY125
ANY500
ANY999125 ml500 ml
1000 ml

Toluidine Blue Solution

TQF125
TQF500
TQF999125 ml500 ml1000 ml

Sodium Hydroxide Solution (3%)

SHC125
SHC500
SHC999125 ml500 ml1000 ml

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CV

P
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands