14Sample Pretreatment,
Accessories

Sample pretreatment ----------------------------------- 136
Column protection -------------------------------------- 137
Tubing parts ----------------------------------------138-139
Guard cartridge -------------------------------------140-141
Empty columns and other parts ---------------------142-143

14

Sample Pretreatment, Accessories

Sample pretreatment, Accessories
Material properties of products
Stainless (SUS-316)

Polyacetal

A general-purpose material for general HPLC products.
Contains Ni, Cr, Mo, etc, as well as Fe, and is highly resistant
to corrosion.

An engineering plastic. A polymer in which aldehyde
compounds are polymerized, with excellent chemical
resistance and excellent machinability.

PEEK (polyetheretherketone)

Polypropylene

A super engineering plastic developed as an alternative tometals.
A polymer containing ketones and ether bonds, it is highlyresistant to pressure, chemicals, heat, and fatigue. Used as
an alternative to stainless for a metal-free tubing system.

A polymer of propylene. Excellent chemical and heatresistance. Often used for containers since processing,such as injection molding, is easy.

Teflon (polytetrafluoroethylene, PTFE)A polymer of tetrafluoroethylene. Highly resistant tochemicals but not so resistant to pressure as PEEK.
Generally used for low pressure tubing systems.
(Teflon is a trademark of DuPont.)

Sample pretreatment
Sample pretreatment cartridge【YMC Dispo SPE】

Method of use
YMC Dispo SPESyringe

ispoCDYMESPDisposable type cartridge made from polypropylene
Utilizes packing material designed for HPLC columns,
manufactured under strict quality controls
Excellent cartridge-to-cartridge reproducibilityHigh recovery of samples

Adapter
VacuumYMC Dispo SPE

Excellent recoverySample

YMC Dispo Cartridge,
Cartridge,
SPECompany A Company B

Basic sample (Propranolol hydrochloride)

89(99)%

59(73)%

66(89)%

Acidic sample (Thioctic acid)91(100)%92(89)%

87(88)%

Vacuum method

Compression method

Centrifugation

Vacuum method is suitable for large-scale treatment and compression
method is suitable for small-scale treatment*Figures inside parentheses represent recoveries obtained when buffer was used.

YMC Dispo SPE C18Container
Made from medical-grade polypropylene
with minimal elution of additives.

Sample Pretreatment, Accessories
136

Upper and lower frits
Pore size of 20 µm

Solid phase

Product No.

Specification

XQSPEC18101

C18 100 mg/1 mL 100/pack

XQSPEC18203

C18 200 mg/3 mL 50/pack

XQSPEC18503

C18 500 mg/3 mL 50/pack

XQSPEC18A06

C18 1000 mg/6 mL 30/pack

YMC＊GELYMC Dispo SPE SIL
Luer tip
Can be connected to commerciallyavailable vacuum solid phaseextraction systems.

Product No.

Specification

XQSPESIL503

SIL 500 mg/3 mL 50/pack

XQSPESILA06

SIL 1000 mg/6 mL 30/packColumn protection【Stainless steel precolumn filter】
Filter construction with minimal dead volume
Effective diameter of the filter is extremely small about 1.6 mm.
Sample diffusion that occurs in passage is minimized.

Stainless steel precolumn filter

FilterProduct No.

Specification

XRPRCS03

For tubing system with 1/16” outer diameter, 1 filter included

XRPRCS35

Spare filter 0.5 μm, 5/pack

Retainer【PEEK precolumn filter】
Metal free
The material of the wetted part of the holder is PEEK and thepacking is PCTFE. The packaging that of the filter is a mixture of
PEEK and Teflon. PEEK precolumn filters are suitable for use in
HPLC systems requiring a metal-free environment.

High-pressure resistance

Filter

PEEK precolumn filters are resistant to ca. 35 MPa.

PEEK precolumn filter
Product No.

Specification

XRPRCP02

For tubing systems with 1/16” outer diameter, 1 filter included

XRPRCP25

Spare filter 2 μm, 5/pack【Handy connector 1】
A ferrule integrated into a connector
 andy connector 1 resists pressure to ca. 21 MPa. Handy connector
H
1 is suitable for tubing systems of relatively low pressure. Connection
to a tubing system is easy because a ferrule is integrated into theconnector.

Metal freeThewhole connector is made from PEEK. Handy connector 1 issuitable for metal-free tubing systems using PEEK tubes.

PEEKHandy connector 1Product No.

Specification

XRP0203

For tubing system of 1/16” outer diameter, 10/packsy andon.
ae,kc
Qui connectiesecur ls required.
No too(1) Extend the top of PEEK tubebeyond the ferrule.

【Handy connector 2】
High pressure resistance and made from stainless steel(2) Force the tube to make tight contact
with the bottom of the screw hole.

Metal free
Theferrule at the wetted part of the connector is made from PEEK.
Handy connector 2 is suitable for metal-free tubing systems using
PEEK tubes.

Stainless steel

Ferrule for replacement

Ferrule is replaceableOnly
the ferrule needs to be replaced when it deteriorates, asopposed to the entire connector.

Handy connector 2

Stainless steel

Product No.

Specification

XRP0204

For tubing system of 1/16” outer diameter, 2/pack

XRP0205

Spare PEEK ferrule, 10/packFerrule for replacement(3) Turn the connector while pushing
the tube to connect the tube andconnector.
(4) Be sure that there is no leak fromthe screw hole.

Sample Pretreatment, Accessories

 andy connector 2 resists pressure to ca. 42 MPa. Handy connectorH
2 is suitable for connection to the injection side of a column, towhich high pressure is applied.
It is also useful for connection of a guard column or a precolumnfilter.

Be aware that Handy connector 2 cannot be connected to “Old Shimadzutype”, “Old JASCO type”, or “Old Hitachi type” tubes.
137

14Sample Pretreatment, Accessories
Tubing parts【PEEK tubing】

Outer diameter 1/16”

Easy to use
 EEK tubing is made from a material that is flexible and easy
Pto use. It is easily cut using a commercially available cutting
knife. A PEEK tube with an inner diameter of 0.127 mm isuseable at up to 40 MPa. PEEK tubes are practical generaltubing as alternatives to stainless steel tubing. They aresuitable for metal-free tubing systems. A Handy connector issuitable for connection of PEEK tubing to a column.

Inner diameter
0.127 mm

【Stainless steel tubing】

0.25 mm

0.5 mmOuter diameter 1/16”

0.8 mmOuter diameter 1/8”

Stainless steel male nut and ferrule are used for connection【Tube cutter】
For cutting 1/16” stainless tubing (simple type)
Inner diameter
0.25 mm

0.5 mm

0.8 mm2.18 mm【Male nut for 1/16” tubing system】(stainless steel)

W type, P type

Old Shimadzu type

Old JASCO type

Old Hitachi typeFemale nut for 1/8”
tubing systemFerrule for 1/8” tubing system【Ferrule for 1/16” tubing system】
(stainless steel)
Male nut for 1/16”
tubing system
Ferrule for 1/16” tubing system【Female nut for 1/8” tubing system】
Sample Pretreatment, Accessories
138(cap nut) (stainless steel)【Union for 1/16” tubing system】【Column coupler】

Unions for 1/16” tubing system come in twotypes, made from either stainless steel or PEEK.
They are convenient to use it with a Handy
connector. Connector type is “W type”.

Column couplers are used for columnto-column connections. Various tube
inner diameters and column connection
types are available.

【Ferrule for 1/8” tubing system】
(stainless steel, double ring)

Stainless steel

PEEK

Cross sectionPEEK tubingnnioforSpecification

XRPT01

1/16” outer diameter, 0.127 mm inner diameter, 3 m length

XRPT02

1/16” outer diameter, 0.25 mm inner diameter, 3 m length

XRPT05

1/16” outer diameter, 0.5 mm   inner diameter, 3 m length

XRPT08

1/16” outer diameter, 0.8 mm   inner diameter, 3 m length

msteygsbintu
6”

Product No.

Stainless steel tubing

1/1UProduct No.

Specification

XRST02

1/16” outer diameter, 0.25 mm inner diameter, 2 m length

XRST05

1/16” outer diameter, 0.5 mm inner diameter, 2 m length

XRST08

1/16” outer diameter, 0.8 mm inner diameter, 2 m length

XRST201/8” outer diameter, 2.18 mm inner diameter, 5 m length

Tube cutter

Notes for successful tubing
The shapes of separation peaks are affected not only by column
performance, but by the tubing system as well.
Some effort is needed to minimize the dead volume in a tubing
system among the injector column and detector, in order to prevent
poor peak shape. Using a tube with a small inner diameter (0.127mm) is one solution. When a tube is connected to a union or acolumn, it is important to give due consideration to make the top of
the tube contact flush with the bottom of the screw hole and to avoid
leakage or creation of a cavity.

Product No.

Specification

XRP1909

For cutting 1/16” stainless tubing

Male nut for 1/16” tubing system (stainless steel)
Product No.

Specification

XRONW

1/16” W type, P type, 5/pack

XRONS

1/16” Old Shimadzu type, 5/pack

XRONJ

1/16” Old JASCO type, 5/pack

XRONH

1/16” Old Hitachi type, 5/packFerrule for 1/16” tubing system (stainless steel)esdgrtri1)
ar14
Gu age(padcrpleoucmn

Product No.

Specification

XRONFA1/16” one ring ferrule, 5/packFemale nut for 1/8” tubing system (cap nut)(stainless steel)
Product No.

Specification

XRON8

1/8” female nut, 5/packlu

ComninMalu
coFerrule for 1/8” tubing system (stainless steel)
Product No.

Specification

XRTWFA

Front ferrule, back ferrule, 5/pack each

Product No.

Specification

XRUNW

1/16” tubing system (stainless steel)XRUN16P

1/16” tubing system (PEEK)

Column coupler
Product No.

Specification

XRCP1602

1/16” outer diameter, 0.25 mm inner diameter

XRCP1605

1/16” outer diameter, 0.5 mm inner diameter

XRCP1620

1/16” outer diameter, 0.8 mm inner diameter

Sample Pretreatment, Accessories

Union for 1/16” tubing system(Stainless tube 50 mm×1, male nut×2, ferrule×2)

139

14

Sample Pretreatment, Accessories
Guard cartridge columns for UHPLCEXP®Guard cartridge holder

High pressure resistance up to 100 MPa (15000 psi)
Low-volume, low-dispersion cartridges minimize the impact on separation
Zero-dead-volume direct connection to column
Hand-tight guard replacement (No tools required)Low-volume, low-dispersion cartridges minimize the impact on separation

EXP®Cartridge column

1mV
150

2Column: YMC-Triart C18 1.9 μm
100 X 2.0 mmI.D.
Eluent: acetonitrile/water (60/40)
Flow rate: 0.4 mL/min
Temperature : 25°C
Detection: UV at 270 nm
Injection: 1 μLSample: 1. Uracil
2. Methyl benzoate
3. Naphthalene
4. Butyl benzoate

34100With guard cartridge
N4 = 19,20050

Main column only
N4 = 19,70000

1.0

2.0

3.0

4.0

5.0 min

EXP® guard cartridge column with low-volume and low-dispersion column minimize the impact on separation.
EXP® guard cartridge column provides less than 3% decrease in theoretical plate count.

EXP®Cartridge Column (Pressure limit: 100 MPa, 3/pack)
Column size
Particle size
inner diameter X length
(μm)
(mm)
1.9

Product number
Triart C18

Triart C18 ExRS

Triart C8

Triart Phenyl

Triart PFP

Triart Bio C4

2.1 X 5TA12SP9-E5Q1CC TAR08SP9-E5Q1CC TO12SP9-E5Q1CC TPH12SP9-E5Q1CC TPF12SP9-E5Q1CC TB30SP9-E5Q1CC

3.0 X 5TA12SP9-E503CC TAR08SP9-E503CC TO12SP9-E503CC TPH12SP9-E503CC TPF12SP9-E503CC TB30SP9-E503CC

A cartridge holder will need to be purchased separately before using this product for the first time.

EXP®Guard Cartridge Holder (Adjustable ferrule, Pressure limit: 137 MPa)
EXP®direct connect holder(for both 2.1 mm and 3.0 mm inner diameter, 2 titanium hybrid ferrule and 1 nut included)

XPCHUHPFitting for Connecting System and Main Column/Guard Cartridge(Adjustable ferrule, Pressure limit: 137 MPa)
Hand-tight EXP® fitting (Hand-tight nut and titanium hybrid ferrule included)

XRHTF-01
Hand-tight EXP® fitting

Replacement Ferrule
Titanium hybrid ferrule (5/pack)Sample Pretreatment, Accessories140

XRHTF-05*EXP® is a registered trademark of Optimize Technologies, Inc.

YMC guard cartridges
Wide variety of packing materials
The packing materials with various bonded phases identicalto those available for analytical columns is packed under highpressure.

Cartridge holder

Disposable

Cartridge column

The cartridge holder can be used repeatedly. Only the cartridge
column needs replacement when worn out.

Column coupler

Easy cartridge replacement
The main body of the YMC guard cartridge is composed of acartridge holder and a cartridge column. The cartridge column
is threaded for easy replacement.

Cartridge Column
Column size
inner diameter X length (mm)2.1 X 10
3.0 X 10
4.0 X 10
2.1 X 20
3.0 X 20
4.0 X 20

Product number*
1/pack-01Q1GC-1-0103GC-1-0104GC-1-02Q1GC-1-0203GC-1-0204GC-1

5/pack-01Q1GC-0103GC-0104GC-02Q1GC-0203GC-0204GC

A cartridge holder will need to be purchased separately before using this product for the first time.

Cartridge Holder Set
cartridge holder set (10 mm length)
cartridge holder set (20 mm length)

XPGCH-Q1
XPGCH-Q2

Cartridge holder set includes a cartridge holder (1 set), and a column coupler.

Guard cartridge columns for semi-preparativePressure resistance up to 30 MPa (4350 psi)
Ideal for protecting semi-preparative column

Cartridge holder
Inner diameter : 10 mm

Cartridge Column (2/pack)
Column size
inner diameter X length (mm)10 X 1020 X 1030 X 10

Cartridge column

Product number*-0110CC-0120CCN-0130CCN

A cartridge holder will need to be purchased separately before using thisproduct for the first time.

Inner diameter : 20, 30 mm

Cartridge holder

Cartridge Holder
XPCHSPW1
XPGHFSP20ID
XPGHFSP30ID* Refer to the page of each product for product number.
Example : Triart C18, 120 Å, 5 μm ⇒ TA12S05

Cartridge columnSample Pretreatment, Accessories

cartridge holder (inner diameter : 10 mm)
cartridge holder (inner diameter : 20 mm)
cartridge holder (inner diameter : 30 mm)

141

14

Sample Pretreatment, Accessories
Empty columns and other parts【YMC original empty columns (for analysis or semi-preparative isolation) 】
A stainless steel tube with a screw and an endfitting are directly connected (screw type). Since YMC original empty columns use no ferrule,
there is no danger of endfitting dislocation when the column is packed and used under high pressure.

YMC original empty columns

Sample Pretreatment, Accessories
142

Product No.

Column size
length X inner diameter (mm)

XC0502WT50

XC1502WT150

XC2502WT250

XC0346WT30

XC0546WT50

XC1046WT100

XC1546WT150

XC2546WT250

XC3046WT300

XC0506WT50

XC1006WT100

XC1506WT150

XC2506WT250

XC3006WT300

XC5006WT

500

XC0308WT30

XC3008WT300

XC5008WT

500

XC0310WT

30

XC0510WT

50

XC1010WT

100

XC1510WT

150

XC2510WT

250

XC3010WT

300

XC5010WT

500

XC0520WT

50

XC1520WT

150

XC2520WT

250

XC3020WT

300

XC5020WT

500

XCA020WT

1000

XCL530WT

75

XC2530WT

250

XC3030WT

300

XC5030WT

500

XCA030WT

1000

Semi-preparative cAnalytical column

2.0

4.6

6.0
Inner diameter2.0-10 mm

8.0
Semi-preparative column

Analytical column

10.0

20.0

Inner diameter2.0-10 mm
30.0

Inner diameter
20-30 mm【Flange type empty column (for preparative isolation)】
A stainless steel tube with a flange and a cap are tightly connected using a bolt. The inner diameter is 50 mm or more.

Flange type empty column

Semi-preparative column

Analytical column

50 mm inner diameter

Product No.

70 mm inner diameter

Column size
Pressure limit
length X inner diameter (mm)(MPa)

Product No.

Column size
Pressure limit
length X inner diameter (mm)(MPa)

XC0551■R

50

XC1071■R100

XC1051■R100

XC2571■R250

XC2551■R250

XC3071■R300

XC3051■R300

XC5071■R500

XC5051■R500

XCA071■R

1000

XCA051■R

1000

XC1072■R100

XC0552■R50

XC2572■R250

XC1052■R100

XC3072■R300

XC2552■R250

XC5072■R500

XCA072■R

1000

50.0

XC5052■R50.0300
Inner diameter
500 mm2.0-10

XCA052■R

1000

XC3052■R

714

Designate a frit pore size depending on particle size.
We recommend 2 μm for particle size below 10 μm,
5 μm for particle size above 15 μm.

100 mm inner diameter
Product No.

70.0

770.0

14

Inner diameter
Designate a frit pore size depending on particle size.
20-30 mm
We recommend 2 μm for particle size below 10 μm,
5 μm for particle size above 15 μm.

150 mm inner diameter

Column size
Pressure limit
length X inner diameter (mm)(MPa)

Product No.

Inner diameter
50-200 mm

200 mm inner diameter

Column size
Pressure limit
length X inner diameter (mm)(MPa)

Product No.

Column size
Pressure limit
length X inner diameter (mm)(MPa)

XC10A1■R100

XC20B1■R200

XC20C1■R200

XC30A1■R300

XC30B1■R300

XC50C1■R

500

XC50A1■R

500

XC50B1■R

500

XCA0C1■R

1000

XCA0A1■R

1000

XCA0B1■R1000

XC20C2■R200

XC10A2■R100

XC20B2■R200

XC50C2■R

500

XC30A2■R300

XC30B2■R300

XCA0C2■R

1000

XC50A2■R

500

XC50B2■R

500

XCA0A2■R1000XCA0B2■R

1000

100.0

100.0

714150.0

150.0714200.0

7200.014*■Connection type: Select your desired connection type.
A : 1/16” male nut
D : 1/8” female nut
E : 1/4” female nut【Airtight stopper】
1/16” airtight stopper
Product No.

Specification

XPMSY

YMC original type (PEEK), 5/pack

XPMSW

Waters type (stainless), 5/pack

1/8” airtight stopper

YMC original type

Waters type1/8” airtight stopper

Product No.

Specification

XPMS8Female nut, 2/packSample Pretreatment, Accessories

Airtight stoppers are used to prevent column contents from drying.
Please use a stopper appropriate for each column connection type.

143