Anti-Laminin gannma-2 antibody, mouse monoclonal (YN557)
Product code

70-352Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Basement membrane isolated from bovine cornea

Isotype

Mouse IgG1κ

Reactivity

Human, Bovine

Special notes

N/A

Application

1. Western blotting: x1/1,000-2,500 (Fig.1)
2. Immunofluorescence microscopy x1/250-500 (Fig.2,3)

Background

Laminins, a family of extracellular matrix glycoproteins, are the major
noncollagenous constituent of basement membranes. Laminins have been
implicated in a wide variety of biological processes including cell adhesion,differentiation, migration, signaling, neurite outgrowth and metastasis, byinteracting with other extracellular matrix components. Laminins areheterotrimeric proteins with a high molecular mass (~400 to ~900 kDa). They
contain three different chains (α, β and γ) encoded by distinct genes, respectively.
Laminin subunit γ-2 in human is encoded by the LAMC2 gene, and known ascell-scattering factor 140kDa subunit (aa 1,193). The epithelium-specificexpression of the γ-2 implied its role as an epithelium attachment molecule, andmutations in this gene have been associated with junctional epidermolysis
bullosa and an important role in cancer invasion.

Data Link

UniProtKB: Q13753 (LAMA3_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.

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Data Images: 70-352 Anti-Laminin gannma-2 antibody, mouse monoclonal (YN557)

CBB YN557(kDa)
200116
9666
42
DJM-1 cell
Conditioned mediumFig. 1 Western blot analysis of YN557 antibody
Conditioned medium prepared from DJM-1 cells was concentrated by ammonia sulfate precipitation,
and was stained with CBB and immunoblotted with YN557 antibody (1:2,500 dilution). The HRPconjugated goat anti-mouse IgG was used as the second antibody. YN557 antibody detected 155 kDa
and 105 kDa bands for non-processed and processed form of laminin γ-2 chains, respectively. Reacted
protein bands were visualized using a chemiluminescent detection with EzWestLumi plus kit (ATTO,Tokyo, Japan).

Fig.2 Immunofluorescence microscopy of human skin
A frozen acetone-fixed human skin section was stained with YN557 antibody (1:500 dilution). The
FITC-conjugated goat anti-mouse IgG was used as the second antibody.The antibody revealed thelocation of laminin γ-2 chain at the dermal-epidermal junction (arrows). E: epidermis, D: dermis. Bar= 50 m.

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Fig. 3 Immunofluorescence microscopy of cultured DJM-1 cells
Methanol-fixed human carcinoma derived DJM-1 cells were stained with YN557 antibody (1:500
dilution, green) and DAPI (cyan). The FITC-conjugated goat anti-mouse IgG was used as the secondantibody. The antibody detected arc- or spotted staining patterns (arrows), which are typical fordeposited laminin-332 in DJM-1 cells. Bar = 20 m.

Reference
1. Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K.

Isolation of ahemidesmosome-rich fraction from a human squamous cell carcinoma cell line. Exp Cell Res., 324:
172-182, 2014.
2. Hirako Y, Usukura J, Uematsu J, Hashimoto T, Kitajima Y, Owaribe K. Cleavage of BP180, a 180kDa bullous pemphigoid antigen, yields a 120-kDa collagenous extracellular polypeptide. J Biol
Chem. 273:9711-9717, 1998
Related product70-350 Anti-Laminin arpha-3 antibody, mouse monoclonal

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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/