2024 PRODUCT CATALOGNEUROPHYSIOLOGICAL PRODUCTS
FOR PRECLINICAL IN VIVO RESEARCHPINNACLE TECHNOLOGY | WWW.PINNACLET.COM
2721 OREGON STREET | LAWRENCE, KS 66046
PHONE: (785) 832-8866 | FAX: (785) 749-9214
SALES@PINNACLET.COM

Thank you for choosing Pinnacle Technology for your research needs. We offer a
range of turn-key systems for neurophysiological studies using freely moving animals andare committed to developing new tools that simplify measurement, reduce cost and enable
new research. In addition, Pinnacle offers a host of supporting products ranging from cages
to software analysis suites. We pride ourselves in providing exceptional customer service andare available to assist you with every stage of your research process.
By forging collaborative relationships with our clients, we are able to develop cutting-edge
tools that improve and simplify your research. We look forward to working with you.
All the Best,

Donna A. Johnson, Founder

INFORMATION & policies
Website: www.pinnaclet.com
Email:

sales@pinnaclet.com

Phone:
Fax:(785) 832-8866

Shipping Address:

2721 Oregon Street(785) 749-9214

				

Lawrence, KS 66046
USA

Ordering Information:
General: Products may be ordered directly from Pinnacle Technology DE, LLC or from one of our approved distributors (see: www.pinnaclet.
com/distributors.html). Some products may not be available in all countries.
Biosensors and Carbon Fiber Electrodes: Pinnacle Technology DE, LLC, requires seven business days notice prior to requested date ofshipment for biosensor or carbon fiber electrode orders.
Payment Terms: Net 30 days from date of invoice for customers with established credit. Prepayment or COD may be required if credit has not been
established. Major credit cards are accepted. Unpaid balances are subject to a late payment fee of 1.5% per month. Pro-forma invoices are available
for international orders.
Use of Products: All Pinnacle Technology DE, LLC products are sold for laboratory research use only. Pinnacle Technology DE, LLC products havenot been approved by any government agency for use in human subjects or human testing.
Shipping Information:
United States: All orders ship F.O.B. Lawrence, KS. Standard orders are shipped FedEx ® Ground (biosensor orders are shipped FedEx ® 2Day).
Freight charges are added to the final invoice.
International: Purchaser is responsible for payment of all import duties, tariffs, taxes, insurance and other related charges. Pinnacle Technology
DE, LLC ships via the purchaser’s courier of choice (UPS®, FedEx ®, DHL®) using the purchaser’s courier account number. Orders WILL NOT BESHIPPED without this information. Pinnacle Technology DE, LLC accommodates orders shipped through domestic shipping brokers.
Discrepancies and damaged goods: Order discrepancies (quantity, type, or damage) must be reported to our sales and technical support
address at sales@pinnaclet.com within 30 days after delivery.
Product/Price Notices: Prices and specifications are subject to change without notice.
Product Return Policy: All product returns require a Return Merchandise Authorization (RMA) number. Contact a Pinnacle Technology DE, LLC
representative to obtain an RMA number and proper RMA documentation. Returns should be shipped to Pinnacle Technology DE, LLC within 30 daysof RMA number issuance. RMA documentation must be included in the return shipment and the customer is responsible for all shipping and handlingcharges. Standard items that have not been used or damaged may be returned within 10 days of original delivery for a credit or refund. A 25% restocking
charge will be deducted from the refund or credit at Pinnacle Technology DE, LLC’s discretion. Pinnacle Technology DE, LLC does not offer refunds orcredits on special, custom, or made-to-order products with custom modifications. All products returned for repair or replacement must be sanitary,cleaned appropriately and securely packaged.
Warranty Information: In general, products are warranted against defects in material and workmanship. Purchasers must comply with Pinnacle
Technology DE, LLC’s policy regarding returns. Refer to Pinnacle Technology DE, LLC’s website (www.pinnaclet.com/general-product-info.html) for
detailed warranty information.

TABLE OF CONTENTS

1-6 SENSORS

21 SYNCHRONIZED VIDEO

1

CNS Biosensors Overview

21

Synchronized Video Capability

2

Biosensors and Guide Cannulas

21

Camera Options

2

Oxygen Sensors

3

Wireless Systems for Rats

4

Tethered Systems for Mice and Rats

5

Subcutaneous System for Rats

6

Desktop Potentiostat

6

Calibration Kits

22-23 Animal HOUSING
22

Animal Housing

23

Group Housing

23

Mix and Match Panel Options

23

Behavioral and Tracking Components

7-13 EEG/EMG SYSTEMS
7

Wireless Systems for Mice and Rats

8

Tethered EEG/EMG Systems Overview

24-25 ADDITIONAL PRODUCTS

Tethered Systems for Mice and Rats

24

Stimulus Controller Kit

10

How Our Preamplifiers Work

24

Illuminators

11

Tethered Three-Channel Systems

25

Commutators

12

Tethered Four-Channel Systems

25

Analog Adapters

13

Headmounts, Depth Electrodes and Screws

25

Isolated Wireless Potentiostat

25

TTL Plus

25

Master Clock

9-10

14 SLEEP DEPRIVATION
14

Sleep Deprivation System

15-18 OPTOGENETICS
15

Optogenetics Systems Overview

16

Wireless Systems for Rats16

Add Optogenetics to FSCV16

LED Fiber Probes

17-18
18

Tethered Systems for Mice and Rats
Hardware and Accessories

19-20 FAST SCAN CYCLIC VOLTAMMETRY19

FSCV Systems Overview

19

Wireless Systems for Rats

19

FSCV Software

20

Tethered Systems for Mice and Rats

20

Carbon Fiber Electrodes

26-28 SOFTWARE
26

Sirenia® Overview

26

Sirenia® Acquisition

26-27

Premium Modules

27

Sirenia® Sleep Pro

27

Sirenia® Seizure Pro

28

Sirenia® Feedback Pro

28

Sirenia® X-Y Tracking

28

Sirenia® Multiple Animal Tracking

SENSORS

CNS biosensorS Overview
CNS BIOSENSORS monitor real-time changes in neurochemical concentrations. With Pinnacle’s turn-key electronic and softwaresystems, users can record and analyze second-by-second concentration changes of neurochemicals in freely moving animals.
Pinnacle currently offers glutamate, glucose, lactate and ethanol biosensors. Our biosensors function by the enzyme-mediatedprocessing of the analyte ofPlatinum-Iridium Inner-Selective
Surface
Membraneinterest. This results in the
productionofperoxide

that

is

then
Integrated Ag/AgCl
Reference Electrode
350 µmPt-Ir electrode. Electroactive

PTFE Coated Electrode
240 µminterferents present in thebrain are excluded via a

Pt-Ir Sensing Cavity
80/176 µm

and through active removal
necessary.

Our

1 mm

passive selective membranesensors are shipped within
sevenbusinessdaysoforder receipt and include awarranty.

Outer-Diffusion
Limiting Membrane*hydrogendetected by oxidation at awhen

EnzymeLayer

Custom

BSA

e-

H2O2

e-

H2O2

e-

H2O2

e-

H2O2

Analyte ofInterest

Epoxy Tip
~200–225 µm

sensorInterferant

sizes are also available.
*Outer-diffusion limiting membrane may not be present on all Pinnacle biosensors

CNS BIOSENSOR CHARACTERISTICS
In Vivo Lifetime

Limit of Detection
180 µmLimit of Detection
80 µm

COMMON USES◊

In vivo monitoring of brain chemical microenvironments◊

Neurochemical monitoring
physiological activities

behavioral

0.02–0.08 µM

0.06–0.3 µM

Glucose96+ hours

1.3–4 µM

1.9–15.2 µM◊

Drug screening, including neuropharmacological effects

Lactate96+ hours

0.2–0.6 µM

1.4–3.5 µM◊

Identification of biomarkers◊

Investigating cognition, behavior, circadian cycles, stress,
learning, memory, sleep, seizure, vigilance state and new
drug effects

6–8 hours

N/A

0.1–0.5 µM

Glutamate Biosensor Linearity

Glutamate Interference Response

Linear18
R2 = 0.9992
16
14

Selective

12
10
8

Fast

6
4
2
0
0

5

10

15

20
25
30
Glutamate (µM)

35

40

45

50

14

2 µM
2 µM
L-Aspartic
L-Glutathione
Acid

12
Oxidation Current (nA)

20

1 µM
GABA

1 µM
5-HT

2 µM
L-Cysteine

10
8
10 µM
Glutamate

6

1 µM
NE

1 µM
DA

2 µM
Tryptophan

2 µM
L-Tyrosine

2 µM
L-Glutamine

250 µM
Ascorbate

600

700

4
10 µM
Glutamate

2
0
0

100

200

300

400
Time (s)500

Linear Response: Responds over a physiologically relevant concentration range at physiologic oxygen levels
High Specificity: Excludes endogenous electroactive interferents present in the brain1

and36 hours

Ethanol

Current (nA)duringGlutamate

Fast Response: Capable of monitoring rapid physiological events

SENSORS

Biosensors & guide cannulas
Biosensors are purchased by size, cannula type and analyte of

CNS Biosensor types

180 μm 80 μm
7001

7001-80

Mouse Biosensor

7004

7004-80

reference) that are compatible with multiple guide cannula types;

Wireless Rat Locking Biosensor

7002

7002-80

our standard configurations use BASi cannulas. Sensors with no

Wireless Rat Friction Fit Biosensor

7007

7007-80

Tethered Rat Locking Biosensor

7011

7011-80

Tethered Rat Friction Fit Biosensor

7012

7012-80

No Cannula Biosensor

interest. Pinnacle recommends the use of a GUIDE CANNULA
system for optimal results when implanting and recording fromsensors in freely moving animals. We manufacture standard
biosensor electrodes (Pt-Ir wire with an integrated Ag/AgClcannula and custom sensors are also available for purchase. All
sensors sold by Pinnacle are for non-human use only.
Pinnacle offers one cannula option for mouse biosensors andtwo cannula options for rat biosensors. When only one cannula
is being used in an experiment, the rat locking cannula is the7001-Glutamate

7001-Ethanol

7001-Glucose

7001-Lactate

All biosensor types can be ordered in any of the analytes/configurations
listed under 7001. For example, a 180 μm glutamate biosensor with a mouse
cannula headpiece is ordered as 7004-Glutamate.

recommended option. However, when using two or more cannulas

Guide cannula types

simultaneously (e.g. 2 biosensors or 1 biosensor/1 LED probe),
the rat friction fit cannula will allow for greater placement freedom.

Mouse Cannula
2.5 mm
6 mm

End of Guide Cannulawith Inserted Biosensor
Guide Cannula Shaft: 0.65 mm

3.2 mmGuide Cannula for Mice

7032

Locking Guide Cannula for Rats

7030

Friction Fit Guide Cannula for Rats

7034

Oxygen Sensors
Pinnacle’s OXYGEN SENSORS can be used with Pinnacle’s

5 mm~1.3 mm from end of guide shaft
to midpoint of sensing cavity
Sensing Cavity: 1 mm

electronics and software to routinely record and analyze secondby-second concentration changes in the brains of freely moving
animals. Our oxygen sensor is a 180 µm disc electrode with an
integrated reference and operates under a -0.6 V bias.

Rat Locking Cannula

Oxygen Sensors

Recommended for 1 cannula experiments

O Ring
4.6 mm19.5 mm

10 mm

End of Guide Cannulawith Inserted Biosensor

Mouse Sensor with Integrated Reference

7004-O2

Wireless Rat Locking Sensor with Integrated Reference

7002-O2

Guide Cannula Shaft: 0.7 mm

Wireless Rat Friction Fit Sensor with Integrated Reference

7007-O2

Tethered Rat Locking Sensor with Integrated Reference

7011-O2

2.5 mm from end of guide shaft
to midpoint of sensing cavity
Heroin, 100 μg/kg (n=9)

2Sensing Cavity: 1 mm

Heroin, 200 μg/kg (n=8)

Rat Friction Fit Cannula
Recommended for 2+ cannula experiments
2.5 mm
6 mm
3.2 mmEnd of Guide Cannulawith Inserted Biosensor
Guide Cannula Shaft: 0.65 mm

Change in [Oxygen] (μM)

0
-2
-4
-6
-8
-10
-12

10 mm

0
~1.3 mm from end of guide shaft
to midpoint of sensing cavity
Sensing Cavity: 1 mm

60

120

180

240

300

Time (s)Intravenous heroin induces rapid oxygen changes in the rat nucleus accumbens.
Colored symbols (4-s mean, ±SEM) are significantly different from the preinjection
baseline. Solis et al. eNeuro (2017) Jun 7;4(3).

2SENSORS

Wireless systems for rats
TWO-CHANNEL WIRELESS BIOSENSOR SYSTEMS are available for recording neurochemical concentrations in freely moving rats.
Both turn-key systems use Bluetooth® technology to wirelessly transmit data to Pinnacle’s Sirenia® Acquisition software via a USB
dongle. They provide a platform for high-capacity biosensor studies and are well-suited for behavioral experiments.

2-channel

1

1

The Rat Hat top protects the system.

2

A low-powered, wireless, two-channel potentiostat applies a bias and transmits
up to three digitized signals to a paired Bluetooth ® USB dongle that interfaces with
Pinnacle’s Sirenia® Acquisition software for data recording.

3

2

4

3

The Rat Hat bottom is affixed to the skull with bone screws and dental acrylic. It
houses the wireless electronics, battery, guide cannulas and biosensors.
Stereotaxically placed guide cannula(s) allow for the insertion of biosensors postsurgery.

Key features
4◊

Up to 2 channels◊

Untethered freely moving animals◊

Transmission radius: 6 m◊

Supports simultaneous biosensor recordings

Also available as a backpack
Wireless Hardware Kits
2-Channel Wireless Biosensor System®

Wireless Accessory Kits

8100-K5

2-Channel Wireless Biosensor Accessory Kit

8100-K7

Bluetooth wireless potentiostat

8172

Rat locking guide cannula (4)7030Drill bit (2)8112Bluetooth® dongle

9054

BASi rat guide cannula holder

7035-R-BAS

Test load (2)8134-20M

Rat Hat top

8107-BLE

Screwdriver for 1/8” screws

8241-S

Rat Hat bottom (4)8108-BLE

Powered USB hub

9005

8111

Battery (pkg. of 5)9033-CR2032

2-Channel +/- Wireless Sensor System

8100-K13

Bluetooth® +/- wireless potentiostat

8172-O2

Bluetooth® dongle

9054

1/8” Screws (pkg. of 12)

All kits include cables for one animal, software and manuals. Biosensors sold separately.

DISPOSABLE

ITEMS

3

Rats
7030

Rat locking cannula8108-BLE

Rat Hat bottom for 2-channel

7034

Rat friction fit cannula

8111-16

1/8” Screws (pkg. of 16)

7040-S

Single-barrel cannula

8112

Drill bit

7041-S

Tri-barrel cannula9033-CR2032

Battery for 2-channel (pkg. of 5)

SENSORS

Tethered systems for mice & RatsThe TETHERED BIOSENSOR SYSTEM features configurable input channels to record neurochemical concentration changes. Thesesystems employ a head-mounted preamplifier for measuring up to two biosensors simultaneously in one animal, providing a turn-key
solution for biosensor recordings in rodents.
1 The data conditioning and acquisition system performs secondary amplification andfiltering before sending data to Pinnacle’s Sirenia® Acquisition software for collection.
1
2 A low-torque commutator allows for unencumbered freedom of movement.

2

3 Head-mounted preamplifiers house two connectors for biosensors. The rigid

connection ensures high-quality, artifact-free data.
4 Stereotaxically placed guide cannulas allow for the insertion of biosensors post-

surgery. Prefabricated headmounts are affixed to the skull with dental acrylic andact as a connection port for the two-channel biosensor preamplifier.

Key features3◊

Low-torque◊Head-mounted amplification◊

Weight: 1.5 g◊

Supports up to 2 simultaneous biosensor recordings

4

Preamplifier Kits
Mouse Preamplifier Kit for Two Biosensors
Guide cannula for mice (12)Bio-only headmount (6)Hardware Kits
Tethered Mouse Biosensor System

8400-K1

8408

18” mounting plate

8426

7033

Flathead screwdriver

82 8241-M 54

7035-M-BAS

23-Gauge needle (6)8254

Test load (2)8143-10M

Mouse preamplifier

8406-2BIO

8209

Powered USB hub

9005

Rat Preamplifier Kit for Two Biosensors

8400-K2

Components of this kit are the same as above except 8408 is
replaced with 8409.

All kits include cables for one animal, software and manuals.
Biosensors sold separately.

Rat locking guide cannula (4)7030BASi rat guide cannula holder

7035-R-BAS

1/8” Bone screws (2 pkgs. of 12)

8111

Mouse cannula

7033

Bio-only headmount8209

0.10” Screws (pkg. of 8)8254

23-Gauge needle

8400-K4-2BIO
Screwdriver for bone screws

8241-SRat preamplifier

8407-BIO

Cable from 8401-8409

8413-R-BIO

Drill bit for bone screws

8112

Backmount adapter for 8218 (4)363 Pedestal base (4)8218

Test load (2)Flathead screwdriver8241-M

Powered USB hub

Mice
7032

8241-F

8401-HR

Commutator/swivel

Tethered Rat Biosensor System

8400-K3-2BIO
Screwdriver for mouse screws

Probe holder for cannula (2)0.10” Screws (3 pkgs. of 8)
Data conditioning and acquisition system

7032

8423
8427-10M
9005

Add Optogenetics

Add Carbon
Fiber Electrodes

See pages 15–18 for details.

(Includes standard and enzyme-modified)See page 20 for details.

4

SENSORS

Subcutaneous System for Rats
Pinnacle’s SUBCUTANEOUS MONITORING SYSTEM captures real-time interstitial measurements in freely moving rats with one-second
temporal resolution. Our turn-key system is designed for easy implantation, allowing for rapid and simple surgery. The subcutaneous
system provides second-by-second data collection for real-time analysis, making it ideal for diabetes and metabolic studies. The sensor
connects to a backpack-mounted wireless Bluetooth® transmitter.

1

2

3

1

A durable, tear-resistant, water-resistant pouch houses the electronics and battery.

2

A low-powered, wireless potentiostat applies a bias and transmits up to two digitized signals
to a Bluetooth® USB dongle that interfaces with Pinnacle’s Sirenia® Acquisition software fordata recording.

3

The sensor penetrates the animal’s subcutaneous space on the dorsal surface and is heldin place with four surgical sutures.

4

The system uses a jacket to secure the pouch and stabilize the sensor.

4

Hardware Specifications

Sub-Q sensor specifications◊

System Weight: 5.9 g◊

Channels: 2◊◊

Battery Life: 25+ days◊

Transmission Radius: 20 ft

Our subcutaneous glucose sensors do not react
to the following interferents: galactose, creatinine,urate, xylose, warfarin, acetaminophen, naproxen,
maltose, xanthine and aspirin.

Sensor Range: 10–500 mg/dL◊

Sensor Life: 17+ days

Hardware Kit
2-Channel Potentiostat Backpack Kit
2-Channel LE Bluetooth® wireless potentiostat backpack
Zinc air battery (pkg. of 4)

8100-K5-BP

9033-AZ675
9052

Bluetooth® dongle

9054

Accessory Kit
2-Channel Potentiostat Backpack Accessory Kit
Test load
Battery remover (plastic)

57006-Glucose

8164

USB extension cable

External Calibration

Raw One Second Data
8100-K7-BP
8134-10M
8156

Rat jacket (2)8165Electronics pouch

8166

Suture packets: 3-0 silk (16)8167

Tegaderm: 1624W (6 cm x 7 cm) (4)8168

18-Gauge needle (4)8169Powered USB hub

9005

Zinc air battery (pkg. of 4)

Subcutaneous Glucose Biosensor

9033-AZ675

Within the Pinnacle software environment, data are under the control of theresearcher. The graph above represents 48 hours of data (gray bar = lights off).

SENSORS

Desktop potentiostat
Pinnacle’s FOUR-CHANNEL DESKTOP POTENTIOSTAT is a cost-effective, easy-to-use and highly accurate system for thedevelopment and use of high impedance, amperometric biosensors and biosensor arrays. It is well-suited for anesthetized animal
experiments, brain slices and other in vitro studies. Each of the system’s four channels has one TTL input, one TTL output and one
analog output. The potentiostat is compatible with Pinnacle’s biosensors and third-party sensors.

Hardware Kit

Compatible withthird-party sensors

Desktop Potentiostat System

System Specifications

8100-K4

Desktop potentiostat

8102N

Sensor adapter cable (4)8109Power supply

8118

BNC to alligator leads cable (4)8125

BNC test load (4)8155-10M◊

Channels: 4◊

Maximum sampling rate: 4 Hz◊

4 TTL inputs

Shorting cap

8155-75◊

Current range: 0–20 µA◊

Bias range: -2.048–+2.048 V◊

4 TTL outputs

Flathead screwdriver8241-M

Powered USB hub

9005

◊

◊

Resolution: 3 fA

2 terminal, fixed potential◊

4 analog outputs

Cables, software and manuals are also included.

Calibration kits
Pinnacle offers a number of IN VITRO CALIBRATION SYSTEMS that allow the simultaneous calibration of up to four biosensors. To
relate the in vivo current changes measured by a CNS biosensor to actual changes in analyte concentration, it is necessary to calibrate
the biosensor at the conclusion of the in vivo experiment.
20

1 g/kg EtOH
Change in [Ethanol] (mM)

15

10

5

0

-5
0

30

60

90

120

150

180

210

240

Time (min)Correlating current to concentration

Calibration Kits
Tethered Mouse In Vitro Calibration Kit

7000-K1-T

Tethered Rat In Vitro Calibration Kit(Use with BAS Cannulas)

7000-K2-T-BAS

Wireless Rat In Vitro Calibration Kit(Use with BAS Cannulas)

7000-K2-W-BAS

Wireless Rat Calibration Kit for Pinnacle Biosensors

7000-K2-W-P

Above: In vivo recordings from two ethanol biosensors implanted
contralaterally in the cortex of a Wistar rat. An ethanol bolus (1 g/kg)
was delivered at the 60-minute mark. Data were transformed to changes
in ethanol concentration based on each sensor’s post-calibration.
Inset: The raw, untransformed current (nA) from the two ethanol
biosensors, as acquired through Pinnacle’s acquisition software.

6

EEG/EMG SYSTEMS

Wireless systems for Mice & Rats
Pinnacle offers THREE-CHANNEL WIRELESS EEG/EMG SYSTEMS for long-term studies in mice and rats. The lightweight, headmounted Bluetooth® wireless amplifier streams data to a computer in real-time using a small USB dongle receiver and the Sirenia®
software suite. When combined with Pinnacle’s prefabricated headmounts, EEG/EMG electrode placement is simple and straightforward
to ensure consistent, reliable results. Our wireless systems use off-the-shelf batteries, making electrophysiology telemetry easy andcost-effective.

Technical Specifications

WIRELESS Hardware Kits
2 EEG/1 EMG Wireless Sleep or Seizure System
Bluetooth® wireless EEG/EMG system
Bluetooth® dongle

8200-K9-SL/SE

Size andWeight8274-SL/SE
8274-D

3 EEG Wireless Seizure System

8200-K9-SE3

3 EEG Wireless Seizure System (Extended Input)

8200-K9-SE3-EI

Components of these kits are the same as above except 8274-SL is replaced with 8274SE3 or 8274-SE3-EI. Kits include software and manuals.

Mice

17.1 x 16.2 x 11.0 mm; 3.8 g

Rats

32.1 x 21.2 x 18.3 mm; 6.8 g

Resolution

12 bits

Input Range+/- 480 mV; EI version +/- 960 mV

Sample Rateup to 1024 samples per second (sps) on each channel

Battery Life*256 - Battery Life 5 + days; 512 - Battery Life 3 + days; 1024 Battery Life 1 + day

Battery life can vary based on bluetooth environment in the recording area.

WIRELESS Accessory Kits For Mice
Wireless Mouse System for Sleep or Seizure
Accessory Kit

WIRELESS Accessory Kits For Rats

8200-K10-SL/
SE

Mouse headmount (4)8201-SS0.10” Screws (pkg. of 8)8209

0.12” Screws (pkg. of 8)

8212

Twin pack of silver epoxy (2)8226Screwdriver for EEG screws
Size 13 Zinc-Air Battery (Box of 60)

8241-F
9033-AZ-PR48

Battery Cap (2)8275

EEG/EMG test source

8249

23-Gauge needle (4)8254Multimeter

8255

Powered USB hub

9005

Supplemental Battery for 8274 Firmware Upgrades

8276

Tablet for 8274 Firmware Upgrades

Wireless Mouse System for Seizure Accessory Kit

8200-K10-SE3/
SE3EI

8200-K11-SL

Rat Hat top for 8274

8107-NRH

Rat Hat bottom for 8274 (4)8108-NRH

Rat Headmount for the 8274 (4)8239-W

1/8” EEG screw with wire leads for rats (16)8247

EEG/EMG test source

8249

Multimeter

8255

Screwdriver for rat bone/EEG screws

8241-S

Drill bit for 1/8” bone/EEG screws

8112

Hex screwdriver for rat hat

8147-A

Powered USB hub
Size 13 Zinc air battery (Box of 60)
Supplemental Battery for 8274 Firmware Upgrades
Tablet for 8274 Firmware Upgrades

8276-Tab

Components of this kit are the same as above except 8201-SS is replaced with 8235-SM.
In addition, it contains 24 0.10” screws with wire leads (8403) instead of products 8209,
8212 and 8226.

7

Wireless Rat System for Sleep Accessory Kit

Wireless Rat System for Seizure Accessory Kit

9005
9033-AZ-PR48
8276
8276-Tab

8200-K11-SE3/
SE3EI

Components of this kit are the same as above except the 8239-W is replaced with the8239-W-SE3

EEG/EMG SYSTEMS

Tethered EEG/EMG SYSTEMS Overview
THREE-CHANNEL and FOUR-CHANNEL BIOPOTENTIAL RECORDING SYSTEMS are available for sleep, seizure and generalbehavioral paradigms in freely moving mice and rats. Both EEG/EMG systems use head-mounted preamplifiers to produce exceptionallyclean waveforms, even during animal movement. The four-channel system provides all the great features of the three-channel systemalong with an extra channel, configuration flexibility and the ability to incorporate simultaneous accelerometer, optogenetics, electricalstimulation, and CNS biosensor measurements.

System features

3-Channel

4-Channel

Available for both mice and rats

P

P

Optimized for sleep and seizure experiments

PP

No cable artifact

P

P

Optional video recording

P

P

Digital input/output controlsPP

Low-pass filters

P

P

Adjustable input range

P

Optogenetics compatible

P

Biosensor compatible

P

Accelerometer compatible

P

Sampling rate up to 20,000 Hz per channelP

TTL as analog inputs

P

Change system configuration via preamplifier

PElectrical stimulation compatible

P

Advantages

COMMONUSES◊

Low noise◊

Synchronized video◊

Advanced analysis tools◊

Turn-key systems◊

Simple surgeries◊

Free acquisition software

zzz

F
S

sleep studies

SEIZURE RESEARCH

DEPTH ELECTRODESCORTICAL RECORDINGSLOCAL FIELD POTENTIAL

Add Biosensors
OR Accelerometer

EEG1
100
0
-100
0.0

cognitive studies

2.0

4.0

6.0

8.0

10.0

See page 12 for details.

2.0

4.0

6.0

8.0

10.0

See pages 15–18 for details.

2.0

4.0

6.0

8.0

10.0

EEG2
100

Add Optogenetics

0
-100
0.0

EMG
100
0
-100
0.0

Two channels of EEG data can be captured alongside EMG data using Pinnacle’s three-channel biopotential
recording system.

Add Electrical Stimulation
See page 24 for details.

8EEG/EMG SYSTEMS

Tethered Systems for Mice & Rats
Our TURN-KEY SYSTEMS are engineered to deliver clean, artifact-free data. EEG and EMG waveforms are amplified and filtered at
the head of the animal by a preamplifier. Signals are then passed through the low-torque swivel to the data conditioning and acquisition
system for final-stage amplification and filtering.
Data are collected using Pinnacle’s free Sirenia® Acquisition software. The software allows users to view EEG/EMG recordings in userdefined time periods, manually score sleep and review seizure events. All data can be configured for export to most spreadsheet anddatabase programs and are compatible with our advanced analysis software packages. See pages 26–28 for additional information on
Pinnacle software.
DATA CONDITIONING AND ACQUISITION SYSTEM
A data conditioning and acquisition
system (DCAS) performs secondary
amplification and filtering before sending
data to Pinnacle’s acquisition software forcollection via a USB connection.

Tethered systems for rats
COMMUTATOR
A P1 Technologies commutator
is mounted above the cage.
The commutator’s two-plug
set-up allows for even rotationof the rotor.

SPECIFICATIONS

3-CHANNEL

4-CHANNEL

Adjustable Sampling Rates

200–2,000 Hz

200–20,000 Hz

Software Configurable
Low-Pass Filters

11 Hz–1 kHz

21 Hz–15 kHz

ADC Resolution

16-bit18-bitTTL Support

4 TTL Input/Outputs

4 TTL Input/Outputs

PREAMPLIFIER
Signals are amplified and filteredat the animal’s head using ourpreamplifiers This ensures thedelivery of clean, artifact-freedata. An 18-inch cable connects
from the preamplifier to thecommutator, and the wires areprotected by a metal springcoil. A P1 Technologies screw
connector is used to secure
the preamplifier to the animal’shead.

HEADMOUNTPrefabricated rat headmounts
use fittings mounted on a
9 X 9 millimeter board with
EEG or EMG electrodewires attached. For 4 EEG
configurations, an additional
two-pin electrode is used.
See page 13 for details.

93-Channel Systems: page 11

4-Channel Systems: page 12

EEG/EMG SYSTEMSHEAD-MOUNTED AMPLIFICATION =CLEANER DATAHow Our preamplifiers Work
Gain and High-Pass filters
Pinnacle’s preamplifiers perform X10 or X100 amplificationof differential measurements between two electrodes.
Each channel also features 0.5, 1.0 or 10 Hz high-passfilters. Use the chart below to identify the exact preamplifier
specifications for each channel type in your selectedconfiguration.
Example: A 2 EEG/1 EMG preamplifier configured for seizure studies inmice has a gain of X100 on all channels, 1.0 Hz high-pass filters on the
EEG channels, and 10 Hz high-pass filters on the EMG channel.

Tethered systems for mice

Gain

High-Pass
Filters

X10 or X100

1.0 Hz

Mouse Configurations
SeizureEEG Channel(s)
Sleep

X100

0.5 HzSeizureX10 or X100

10 Hz

Sleep

X100

10 Hz

Rat Configurations

Gain

High-Pass
Filters

SeizureX10

1.0 Hz

Sleep

X100

0.5 HzSeizure

X10

10 Hz

Sleep

X100

10 Hz

COMMUTATOREMG Channel

A low-torque commutator(< 2 x 10 -4 N-m), mounted
above the cage, allows forunencumbered
freedomofmovement. A seven-inch cable
extends from the commutator.

EEG Channel(s)EMG Channel

PREAMPLIFIER
Signals are amplified and filteredat the animal’s head using ourpreamplifiers. This ensures thedelivery of clean, artifact-free data.
The cable from the commutatorconnects to the seven-inch cableof the preamplifier. Six insulated
wires are banded together tocreate this lightweight cable. The
mouse preamplifier connects to aheadmount via a friction fit.

HEADMOUNTPrefabricated headmounts reduce
surgery time, allow for reproducible
electrode placement and provide
ready-to-insert EMG leads. Sixor eight-pin headmounts support
flexible electrode placement forcustomizable cortical or depth
recordings. See page 13 fordetails.

Shared and fully REFERENTIAL/DIFFERENTIAL
Channels
Our standard three and four-channel preamplifiers have two
referential channels and one or two differential channels. Fully
referential and fully differential versions are also available.
See diagrams below. Perspective: Pins extending frompreamplifier

3-Channel System
Standard Configuration(For Mice)
3-Channel System
Fully Independent Configuration(For Mice)

4-Channel System
Standard Configuration(For Mice)

CHANNEL 1

CHANNEL 3

COMMONCHANNEL 2

CHANNEL 4

GROUNDCUSTOM CONFIGURATIONS AVAILABLE
Contact a Pinnacle representative atsales@pinnaclet.com or (785) 832-8866

10

EEG/EMG SYSTEMSTETHERED THREE-CHANNEL Systems
Pinnacle’s THREE-CHANNEL SYSTEM allows researchers to simultaneously record three channels of EEG and/or EMG data.
The data conditioning and acquisition system (DCAS) and preamplifier are preconfigured and ordered as a matching pair. Standard
configurations include 2 EEG/1 EMG and 3 EEG. Fully independent preamplifiers and 3 EEG kindling systems for rats are alsoavailable. Learn more about preamplifiers and how our turn-key systems work on pages 9–10.

Systems for rats
Hardware Kits

Accessory Kits

2 EEG/1 EMG for Sleep or Seizure

8200-K2-SL/SE

3 EEG for Seizure

8200-K2-SE3

2 EEG/1 EMG for Sleep or Seizure

Simple surgeries
8200-K4-SL/SE

Drill bit

8112

Test source

8249

Rat headmount (4)8239

Multimeter

8255

Screwdriver for 1/8” screws

8241-SPowered USB hub

9005

1/8” Screws with wire leads (16)8247

Anterior

Contents:
8206-HR: Data conditioning and acquisition system
8213: Rat preamplifier
8214: Rat commutator/swivel
8258-723: 14” Mounting plate

3 EEG for Seizure

All kits include cables for one animal, software and manuals.
Note: 8206-HR and 8213 come in three varieties: SL, SE and SE3.

8200-K4-SE3

Components of this kit are the same as above except for the quantity of 8247 (24).
In addition, 8239 is replaced with 8239-SE3.
Posterior

2 EEG/1 EMG for Rats

Systems for mice

Accessory Kits
2 EEG/1 EMG for Sleep or Seizure

Hardware Kits
2 EEG/1 EMG for Sleep or Seizure

8200-K1-SL/SE

3 EEG for Seizure

8200-K1-SE3

2 EEG/1 EMG for Sleep or Seizure*
3 EEG for Seizure*

Mouse headmount (4)8201

Test source

8249

0.10” Screws (pkg. of 8)

8209

23-Gauge needle (4)82540.12” Screws (pkg. of 8)

8212

Multimeter

8255

Silver epoxy

8226

Powered USB hub

9005

Screwdriver for EEG screws

8241-F

3 EEG for Seizure

Contents:
8202: Mouse preamplifier
8204-723: Mouse commutator/swivel
8206-HR: Data conditioning and acquisition system8258-723: 14” Mounting plate

2 EEG/1 EMG for Sleep or Seizure*

2 EEG/1 EMG for Mice

8200-K3-SE3

All accessory kits contain items needed for completion of initial surgeries.
All quantities are one unless otherwise noted after the product description.

Anterior

8200-K3-iSL/iSE

Components of this kit are the same as above except 8201 is replaced with 8431SM. In addition, it contains an 8-pin to 6-pin adapter (8272) and 20 0.10” screws
with wire leads (8403) instead of products 8209, 8212 and 8226.

3 EEG for Seizure** Fully Independent

ITEMS

Posterior

Components of this kit are the same as above except 8201 is replaced with 8235SM. In addition, it contains 24 0.10” screws with wire leads (8403) instead of
products 8209, 8212 and 8226.

All kits include cables for one animal, software and manuals.
Note: 8202 and 8206-HR come in multiple varieties: SL, SE, SE-10,
SE3, SE3-10, DSL*, DSE* and DSE3*.

118200-K3-SL/SE

8200-K1-iSL/iSE
8200-K1-iSE3

DISPOSABLE

Anterior

8200-K3-iSE3

Components of this kit are the same as above except 8201 is replaced with 8415SM. In addition, it contains an 8-pin to 6-pin adapter (8272) and 28 0.10” screws
with wire leads (8403) instead of products 8209, 8212 and 8226.

Rats
8111

Posterior

3 EEG for MiceMice1/8” Bone screws

8209

0.10” Screws (pkg. of 8)

8112

Drill bit

8210

0.08” Screws (pkg. of 8)

8247

1/8” Screws with wire leads

82120.12” Screws (pkg. of 8)8425

2-Pin electrode8226

Silver epoxy

8254

23-Gauge needle

8403

0.10” Screws with wire leads

Add synchronized Video

8403-HP

8403 with headmount pins

See page 21 for details.

8405

0.08” Screws with wire leads

EEG/EMG SYSTEMSTETHERED FOUR-CHANNEL Systems
The FOUR-CHANNEL SYSTEM supports up to four biopotential input channels. This system is among the most flexible of Pinnacle’s
hardware devices because the configuration can be easily modified by changing only the preamplifier. Users can add biosensors,
accelerometers, optogenetics or electrical stimulation.

Systems for rats

Preamplifier Kits
2 EEG/1 EMG/1 Biosensor for Sleep or Seizure

Hardware Kit
Pinnacle’s prefabricated andstandard headmounts provide
fast and easy solutions forconnecting
electrodes
topreamplifiers. Stainless steelscrews affix to the skull, doublingas anchors and electrodes for
EEG data acquisition. Depthelectrodes can be solderedtoheadmounts
for
LFP
recordings. For configurations
supporting muscle movement,
EMG leads easily insert into theback or neck muscles.

Anterior

2 EEG/1 EMG/1 Accelerometer for Sleep or Seizure
Rat EEG/EMG System

8400-K2

Data conditioning and acquisition
system8401-HR

3 EEG/1 EMG for Seizure

8409

18” Mounting plate8426

Kit includes cables for one animal, software andmanuals.

4 EEG for Rats

Anterior

8400-K4-SE3

3 EEG/1 Accelerometer for Seizure

8400-K4-SE3-AXL

4 EEG for Seizure

8400-K4-SE4

4 EEG Fully Referential

8400-K4-SE4-REF

2 Biosensor

8400-K4-2BIO

All preamplifier kits include one preamplifier, one-time purchase items and surgical disposables
to complete four surgeries. Biosensors sold separately.

Systems for micePreamplifier Kits

Hardware Kit

Posterior

Mouse EEG/EMG System

8400-K1

Data conditioning and acquisition
system8401-HR

Mouse commutator/swivel

8408

18” Mounting plate8426

Kit includes cables for one animal, software andmanuals.

2 EEG/1 EMG/1 Biosensor for Sleep or Seizure

8400-K3-SL/SE

2 EEG/1 EMG/2 Biosensor for Sleep or Seizure

8400-K3-5SL/5SE

2 EEG/1 EMG/1 Accelerometer for Sleep or Seizure

8400-K3-SL/SE-AXL

3 EEG/1 EMG for Seizure

8400-K3-SE31M

3 EEG/1 Biosensor for Seizure

8400-K3-SE3

3 EEG/2 Biosensor for Seizure

8400-K3-5SE3

3 EEG/1 Accelerometer for Seizure

8400-K3-SE3-AXL

4 EEG for Seizure

8400-K3-SE4

4 EEG Fully Referential for Seizure

Have your own amplifier?

Posterior

4 EEG for Mice

8400-K4-SL/SE-AXL
8400-K4-SE31M

3 EEG/1 Biosensor for Seizure

Rat commutator/swivel

8400-K4-SL/SE

8400-K3-SE4-REF

All preamplifier kits include one preamplifier, one-time purchase items and surgical disposables
to complete initial surgeries. Biosensors sold separately.

Combined EEG/EMG/Biosensor Systems

Learn more about using
Pinnacle’s preamplifiers withthird-party systems on page 25.

Lactate
100 µM
GlucoseAdd Electrical stimulation
See page 24 for details.

Add Optogenetics
See pages 15–18 for details.

EEG

250 µV
EMG

0

0.5

1.0

Hours

1.5

2.0

2.5

EEG and EMG waveforms are plotted simultaneously with calibrated biosensor
traces for lactate and glucose recorded from a single animal.

12

EEG/EMG SYSTEMS

Headmounts
Pinnacle offers a variety of HEADMOUNT CONFIGURATIONS that simplify surgery and provide reliable, reproducible, low-noise
EEG/EMG connections. Additional items are also available to support the headmount surgery. Conduct surgeries using Pinnacle’s
step-by-step guides or adapt them into your own surgical protocol.

Prefabricated Headmounts for Rats

Prefabricated Headmounts
PREFABRICATED HEADMOUNTS for mice andrats reduce surgery time and allow for reproducible
electrode placement. Mouse headmounts consist of asix- or eight-pin connector soldered to a board. They
contain holes for EEG attachments and are available
withpre-soldered,

ready-to-insert depth electrodes or EMG
leads. Rat headmounts are pre-Tethered

Wireless

Wireless withVia Holes

8239

8239-W8239-W-VH

8239-SE38239-W-SE3

8239-W-SE3-VH

2 EEG/1 EMG
Headmount
3 EEG HeadmountPrefabricated Headmounts for Mice
Bio-Only Headmount7033

2 EEG/1 EMG Headmount with Platinum Iridium Leads

8201

2 EEG/1 EMG Headmount with Stainless Steel Leads

8201-SS

soldered with EMG leads and/or
EEG wires.

Standard Headmounts
STANDARD HEADMOUNTS for mice allow flexible

2 EEG/1 EMG Headmount with Stainless Steel Leads (270°)8201-SS-270

3 EEG Headmount (No EMG Leads)

8201-EEG

3 EEG / 4 EEG REF with Via Holes Headmount8201-6P

3 EEG / 4 EEG REF with Via Holes Plus Layout Headmount8201-X

2 EEG/1 EMG/Biosensor Headmount with Pt-Ir Leads

8402

electrode placement for customizable cortical depth
recordings. Use these with Pinnacle’s screws withwire leads to simplify

2 EEG/1 EMG/Bio Headmount with Stainless Steel Leads

8402-SS

soldering during surgery.

2 EEG/1 EMG/Bio Headmount with Stainless Steel Leads (90°)

8402-SS-90

Pre-soldered

EEG

or

Standard Headmounts for Mice

EMG leads are available.

CUSTOM Headmount AND STERILIZATIONSERVICES AVAILABLE
Contact a Pinnacle representative atsales@pinnaclet.com or (785) 832-8866

6-Pin Surface Mount Headmount with EMG Leads

8231-SM

6-Pin Headmount

8235

6-Pin Surface Mount Headmount8235-SM

6-Pin Headmount (No Pins)

8235-OP

8-Pin HeadmountScrews8-Pin Surface Mount Headmount8415-SMSCREWS help anchor the headmount to the skull and function

8-Pin Headmount with EMG Leads

8431

as recording electrodes. Mouse screws are available in

8-Pin Surface Mount Headmount with EMG Wires

packages of eight and rat screws are available in packages of
12. Mouse and rat screws with wire leads are sold individually.

8431-SM

Depth Electrodes
Pinnacle has several DEPTH ELECTRODE options available.

Screws13

8415

They can be purchased individually, pre-soldered to headmounts,

0.08” Mouse Screws (pkg. of 8)8210

or soldered to cannulas for deep brain recordings during optical

0.10” Mouse Screws (pkg. of 8)

8209

stimulation or biosensor recordings.

0.12” Mouse Screws (pkg. of 8)

8212

Depth Electrodes

Mice0.125” Rat Screws (pkg. of 12)

8111

Depth Electrode + Prefabricated Headmount8201-DEP

0.10” Mouse Screw with Wire Lead

8403

Depth Elec. + Prefab. Headmount Stainless

8201-DEP-SS

0.08” Mouse Screw with Wire Lead

8405

Depth Electrode8425-M

8425

0.125” Rat Screw with Wire Lead

8247

Depth Electrode + 8235-SM Headmount8443-M

8443-R

Rats

SLEEP DEPRIVATIONSLEEP DEPRIVATION SYSTEM
Pinnacle’s AUTOMATED SLEEP DEPRIVATION SYSTEM is a unique solution for sleep deprivation and fragmentation studies. It
provides user controls for sleep-depriving mice and rats without direct human intervention. Sleep deprivation simulates gentle handlingby a rotating bar placed a short distance above the cage floor, lightly nudging the animal from sleep and encouraging the animal tomaintain wakefulness without excessive exercise. The system is sold either as a core system or with Pinnacle’s Sirenia® Feedback Pro
software and EEG hardware. Adding Feedback Pro allows real-time EEG/EMG signals to be used to determine sleep/wake state andinitiate deprivation as required.

System features

Core System + Feedback

Available for both mice and rats

P

P

Adjustable speed and motor control

P

P

Calendar-based schedulingP

P

Suitable for short-term, long-term and chronic studies

P

P

Optional video recordingP

P

Compatible with third-party systems

P

Real-time biopotential analysis and feedbackP

Rule-based programmingP

Yoked control functionality

P

Requires Pinnacle’s EEG/EMG systemP

ADVANTAGES
Simulates gentle handling
Prevents sleep acclimation and habituation
Minimizes resources compared to manual deprivation
Reduces unnecessary exercise

Sleep deprivation system with EEG, video and standSleep Deprivation Systems

Zzz

COMMONUSESSIMULATEDSHIFT WORKAUTOMATEDDEPRIVATIONSLEEP FRAGMENTATION

Sleep Deprivation for Mice (10” Cage)

9000-K5-S

Sleep Deprivation for Rats (12” Cage)

9000-K6-S

Sleep Deprivation for Rats (14” Cage)

9000-K6-S-14

Core system

Feedback Pro

The core system provides calendar-based functionality for

Feedback Pro software provides calendar-scheduling functionality

programming the bar to rotate at discrete intervals. Programming

plus the capability of adding real-time EEG/EMG feedback tooptions range from a second-by-second basis to hourly, daily,

ensure the bar rotates only when the animal enters a sleep-

weekly or monthly intervals. Use the device’s touchscreen to

like state. Bar rotation starts and stops automatically based on

operate the system without a computer connection. The systemuser-established rule sets for the animal’s sleep state and users

is compatible with most EEG/EMG hardware and physiologicalcan easily incorporate delays, shifts in bar rotation and timemeasurement systems.

restrictions into the experimental set-up.

Add SYNCHRONIZED VIDEO TO YOUR SYSTEM
See pages 21 for details.

14

OPTOGENETICS

Optogenetics Systems Overview

Key features

Pinnacle’s user-friendly, turn-key OPTOGENETICS systems provide
simple solutions for a wide range of experimental designs. Our
hardware and software platforms seamlessly integrate optogenetic
controlwithsimultaneous

biopotential,

neurotransmitterandbehavioral recordings. The system uses LED fiber probes that arecompatible with standard cannula placement techniques. Tethered◊

Precision timing◊

TTL functionality◊

Optogenetic stimulation with EEG/biosensor recordings◊

Power > 100 mW/mm2 with most LED probes (depending
on wavelength)

solutions are available for both mice and rats, and wireless options
are available for rats.

Glutamate (μM)

Kindling Seizures Using Optogenetics
+1.0

-1.0

EEG 1 (μV)

+500

Depth
Electrode (μV)

EEG 2 (μV)

-500
+500

-500
+300

Stim ON

-300

1:00

2:00

3:00

Minutes

Cortical EEG and hippocampal depth electrode activity with prefrontal cortex glutamate changes during kindlingstimulation with blue (445 nm) light in a transgenic mouse from Jackson Laboratory expressing channelrhodopsin.
Light pulses (20 Hz, 10% duty cycle, 600 mA) were delivered for 20 seconds. The blue LED power measured prior
to implant was 130 mW/mm2.

Altering Sleep Using Optogenetics
EEG 1
5000
Stim ON

Stim ON

Stim ON

Stim ON

Stim ON

Gamma (25-50 Hz) Power (μV2/Hz)

4000
3000
2000
1000
EEG 2
5000
4000
3000
2000
1000
0800

1600

2000

Time

Application of 40 Hz stim frequency (10% duty cycle, 600 mA) 10 seconds ON, 10 seconds OFF periods
for one hour intervals (blue bars) results in increased power in the Gamma frequency (25-50 Hz) range anddecreased NREM sleep by an average of 42 percent during time when stim was applied.

15

OPTOGENETICS

Wireless Optogenetics Systems for Rats
Pinnacle’s WIRELESS BLUETOOTH® OPTOGENETICS SYSTEMS for rats are capable ofdriving our LED fiber probes at the rated 600 mA pulse current. The device is built in a flexboard format for easy use with our standard rat headmount enclosures. In conjunction with theBluetooth® dongle, the device can be configured and all data recorded via the Sirenia® software
suite.

Wireless Hardware Kits
1 or 2 Opto

8400-K19-Opto

2 Opto rat potentiostat
Bluetooth dongle

Wireless ACCESSORY Kits®

1 or 2 Opto Accessory Kit

8489-2opt

8400-K21-Opto

Rat Hat top

8507

8489-D

Rat Hat bottom

USB extension cable

9052

Zinc air battery (pkg. of 4)9033-AZ675

2 Opto/1 Biosensor

8400-K19-O2/BIO

Guide cannula

7032 or 7034

Components of this kit are the same as above except 8489-2opt is replaced with
8489-2opt1Bio.

2 Opto/1 Accelerometer

8400-K19-O2-AXL

8508

1/8” Screws (pkg. of 12)

8111

Screwdriver for 1/8” screws

8241-S

2 Opto/1 Biosensor Accessory Kit

Components of this kit are the same as above except 8489-2opt is replaced with
8489-2opt1bio1axl.

8400-K21-O2/BIO

Components of this kit are the same as above.

2 Opto/1 Accelerometer Accessory Kit
2 Opto/1 Bio/1 Accelerometer

8400-K21-O2-AXL

8400-K19-O2-BIO-AXL
Components of this kit are the same as above.

Components of this kit are the same as above except 8489-2opt is replaced with
8489-2opt1bio1axl.

2 Opto/1 Bio/1 Accelerometer Accessory Kit

1 Opto/2 EEG/1 EMG for Sleep

Components of this kit are the same as above.

8400-K19-O1/2EEG/1EMG

Components of this kit are the same as above except 8489-2opt is replaced with
8489-1optSL.

1 Opto/2 EEG/1 EMG for Sleep Accessory Kit

8400-K21-O2-BIO-AXL

8400-K21-O1/2EEG/1EMG

Components of this kit are the same as above. In addition, it contains a test source (8242), screws withwire leads (8247) and a multimeter (8255).

All kits include cables for one animal, software and manuals. LED fiber probes
and biosensors sold separately.

Add Optogenetics to FSCV
Pinnacle’s turn-key optogenetics and fast scan cyclic voltammetry (FSCV) system offers a simple, straightforward solution for a wide
range of optogenetics experiments in conjunction with FSCV in both rats and mice. Researchers can seamlessly integrate optogenetics
with simultaneous voltammetric recordings enabling precise control of complex neuronal circuitry activation and monitoring. The
stimulus output on any of Pinnacle’s optogenetics FSCV systems can be used for either optogenetics or conventional electricalstimulus, providing optimal flexibility. See pages 19-20 for information about FSCV.

LED Fiber Probes
LED FIBER PROBES

Pinnacle’s optogenetic stimulation uses LEDs
coupled to 200/250 µm fiber optic stub. Fiber
length is 0.5 millimeters beyond the cannula.

Mouse

Rat Locking

Rat Friction

POWER (mW/mm2)
Peak Wavelength (nm)@ 300 mA

@ 600 mA

7080-445-A

7081-445-A

7082-445-A

445 Blue

68

110

The LED FIBER PROBES are implanted using

7080-465-A

7081-465-A

7082-465-A

465 Blue62

101

515 Green

34

60

a guide cannula and standard stereotaxic

7080-515-A

7081-515-A

7082-515-A

techniques. The assembly plugs directly into an

7080-590-A

7081-590-A

7082-590-A

590 Yellow

78

108

electrical header on the headstage, eliminating

7080-620-A

7081-620-A

7082-620-A

620 Amber

77

145

the need for an optical commutator. Custom

7080-640-A

7081-640-A

7082-640-A

640 Red82

104

lengths are also available. See page 2 for

7080-660-A

7081-660-A

7082-660-A

660 Deep Red

97

163

cannula descriptions.

*Not recommended.

16

OPTOGENETICS

Tethered Systems for mice & Rats
Our TETHERED OPTOGENETICS SYSTEMS use a stimulus controller module that controls the frequency, duration and intensity ofillumination events. As the system’s key component, it drives the headstage-mounted LED probes through an electrical commutator(no optical commutator required). Pinnacle’s LED fiber probes are compatible with standard cannulas and surgical techniques.

DATA CONDITIONING AND ACQUISITION SYSTEMSTIMULUS CONTROLLER MODULE
The stimulus controller module
offers precise control of opticalstimulation frequency, duration andintensity for both continuous anddiscrete events.
•••

Highly accurate clock ensures < 1 ppm precision timing
Capable of optical or electrical stimulusStore and run two independent stimulus paradigms

Tethered systems for rats
COMMUTATOR
A P1 Technologies commutator’s
two-plug set-up allows for evenrotation of the rotor. Since Pinnacle
systems place the light sourceat the headmount, no opticalcommutator is needed.

HEADSTAGE
Signals are amplified and filteredat the animal’s head using ourpreamplifiers,which

ensurethedelivery of clean, artifact-free data.
An 18-inch cable connects fromthe preamplifier to the commutator.
The cable’s wires are protected by ametal spring coil. A P1 Technologies
screw

connector

securesthepreamplifier to the animal’s head.

HEADMOUNTPrefabricated rat headmounts use
fittings mounted on a 9 X 9 millimeterboard with EEG or EMG electrodewires attached. An additional twopin electrode is used for 4 EEG
configurations.

17

A data conditioning and acquisition
system (DCAS) performs secondary
amplification and filtering before sending
data to Pinnacle’s acquisition software
for collection via a USB connection.

OPTOGENETICS

Hardware & accessories
The optogenetics system is sold in three kits. HARDWARE KITS for mice and rats include all system components except theinterchangeable headstages. HEADSTAGE KITS include a headstage and EEG/biosensor-related surgical and testing supplies.
Lastly, an OPTOGENETICS ACCESSORY KIT includes optogenetic-specific surgical and testing equipment.

SYNCHRONIZED VIDEO (OPTIONAL)
Our optional synchronized video
system adds video of an animal’s
behavior to the biopotential andbiosensor data. The system includes
everything you need to add video
capture to your research. See page
21 for details.

Hardware kits

Mice

Rats

Optogenetics Biopotential System

8400-K11

8400-K12

Data conditioning and acquisition system

8401-HR

8401-HR

Stimulus Controller module

8480-SC

8480-SC

Commutator

8481-M

8214-KIN

8400-K15

8400-K16

Optogenetics Only System

The components of this kit are the same as above, except the 8401-HR is not included.
All kits include cables for one animal, software and manuals.

Tethered systems for MiceCOMMUTATOR
A low-torque commutator (< 0.002
Nm) allows for unencumbered
movement for mice. Since Pinnacle
systems place the light source at theheadmount, no optical commutator isneeded. A seven-inch cable extends
from the commutator.

HEADSTAGE
Our headstages amplify and filter thesignal at the headmount to deliverclean, artifact-free data. A secure
friction fit connects the mouseheadstage to the headmount. A
seven-inch cable extends from theheadmount, connecting to the seveninch cable of the commutator. Six
insulated wires are banded together
to create this lightweight cable.

Headstage Kits

Mice

Rats

1 Stim/1 Biosensor

8400-K13-O1/1BIO

8400-K14-O1/1BIO

1 Stim/2 EEG/1 EMG for
Sleep

8400-K13-O1/SL

8400-K14-O1/SL

2 Stim/2 EEG/1 EMG for
Sleep

8400-K13-O2/SL

8400-K14-O2/SL

1 Stim/2 EEG/1 EMG/1
Bio for Sleep

8400-K13-O1/SL/BIO

8400-K14-O1/SL/BIO

1 Stim/3 EEG for Seizure

8400-K13-O1/SE3

8400-K14-O1/SE3

2 Stim/3 EEG for Seizure

8400-K13-O2/SE3

8400-K14-O2/SE3

1 Stim/3 EEG/1 Bio for
Seizure8400-K13-O1/SE3/BIO 8400-K14-O1/SE3/BIO

1 Stim/4 EEG for Seizure

8400-K13-O1/SE4

8400-K14-O1/SE4

1 or 2 Stim Only

8400-K17-Opto

8400-K18-Opto

All headstage kits include one headstage, one-time purchase items and surgical disposables
to complete initial surgeries. LED fiber probes and biosensors sold separately.

Accessory Kits
Optogenetics

Mice

Rats

8400-K28

8400-K29

Optogenetics Friction Fit

8400-K30

Includes optogenetics-specific surgical and testing equipment.

HEADMOUNTPrefabricated headmounts reduce
surgery time, allow for reproducible
electrode placement and provide
ready-to-insert EMG leads. Multiple
configurations are available, with
EMG placement at 90° or 270°
rotation. Custom configurations areavailable, as well.

already have pinnacle’s
8401 data acquisition system?
Add Optogenetic and electricalstimulation to Your existing setup with
our expansion kits on page 24
18

FSCV

FSCV Systems Overview
Pinnacle’s robust, turn-key FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEMS are designed to simplify the detection andquantification of catecholamines and other electroactive analytes. It functions by rapidly cycling a voltage across an implanted carbon
fiber electrode and measuring the resultant current. All of Pinnacle’s FSCV systems (tethered and wireless) are compatible with
Pinnacle’s stimulus controller. The system comes with Pinnacle’s Sirenia® FSCV software.

Key featuresOXIDATION
REDUCTION

Wireless Systems FOR rats

System Specifications◊

Background subtraction◊

3D visualization◊

User-selectable filters◊

Data export◊

Video recording

Max points/sweep: 1000◊

Custom waveforms◊

Real-time color plots

Standard sweeps: Dopamine,
Serotonin, Norepinephrine, Adenosine

Voltage span: -0.6 – +1.5 V
Max sweeps/second: 10
Max scan rate: 1000 V/s

WIRELESS RAT SYSTEMS transmit data from multiple animals to a single computer using Bluetooth®. A head-mounted enclosure
makes the battery easily accessible and exchangeable to support extended recordings. The system is ideal for mazes, metabolic andbehavioral chambers, as well as enclosed environments.

Wireless Hardware Kits
34 μm Wireless Rat System

Wireless Accessory Kits
8500-K2

Components of this kit are the same as above except 8501-7 is replaced with 8501.

Wireless Rat FSCV + Opto System

8500-K12

Components of this kit are the same as above except 8501-7 is replaced with 8501-Opto.
All kits include cables for one animal, software and manuals. Carbon fiber electrodes and
Ag/AgCl reference electrodes are sold separately.

FSCV Software
The

free

FSCV

34 μm Wireless Rat Accessory Kit

8500-K4

Components of this kit are the same as above except 8134-1G and 8134-1M are replacedwith 8134-100K and 8134-100M.

Wireless Rat FSCV + Optogenetics Accessory Kit

8500-K13

Components of this kit are the same as above except 8134-1G and 8134-1M are replacedwith 8134-100K and 8134-100M. In addition, it contains a rat opto cannula holder(7035-R-LED) and a powered USB hub (9005).

Dopamine Response in a flow cell

SOFTWARE

offerspreprogrammed and custom waveforms
associated with the commonly studied
analytes:

dopamine,

serotoninandnorepinephrine,

adenosine.

Customwaveforms can be easily uploaded andstored for quick selection.
The FSCV software supports traditional,
short recording paradigms (recordings of
two minutes or less), as well as longerterm

recordings

using

an

extended,Representative data from a flow cell. Top left: Real-time color plot. Bottom from left to right: Color plot, current response, voltammogram.

continuous mode.

DISPOSABLE

ITEMS

19

Rats
8111

Mice
1/8” Screws (pkg. of 12)7033

Bio-only headmount

8112

Drill bit8212

0.12” Screws (pkg. of 8)8508

Rat Hat bottom

8254

23-Gauge needle

FSCV

Tethered Systems for Mice & RatsTETHERED FSCV SYSTEMS allow researchers to detect and quantify neurotransmitter release and uptake in real-time. A headmounted FSCV board sends signals through a low-torque commutator to an interface box that streams data to the host computer.

Tethered Hardware kits

Mice

Rats

7 μm Tethered System

8500-K1-7

8500-K6-7

34 μm Tethered System

8500-K1

Tethered FSCV + Opto System

8500-K11

Mice

Rats

7 μm Tethered Accessory Kit

8500-K3-7

8500-K7-7

8500-K6

34 μm Tethered Accessory Kit

8500-K3

8500-K7

8500-K10

Tethered FSCV + Opto Accessory Kit

8500-K15

8500-K14

All kits include cables for one animal, software and manuals. Carbon fiber electrodes, Ag/
AgCl reference electrodes and LED fiber probes are sold separately.

Calibration Kit for Rats and Mice
FSCV Calibration Kit

Tethered Accessory kits

Kits include one-time purchase items and surgical disposables to complete initial surgeries.

Stimulator Kits

8500-K5FSCV Stimulator Kit

Tethered Wireless
8500-K8

8500-K9

Carbon Fiber Electrodes
CARBON FIBER ELECTRODES (CFEs) are used with all FSCV systems and come in two diameters: 7 μm and 34 μm. The 7 μm areused only in tethered systems and the 34 μm is compatible with both potentiostats to measure the presence of biogenic amines in thebrain using fixed potential amperometry (FPA). All Pinnacle CFEs require an Ag/AgCl reference electrode.

7 μm Carbon Fiber Electrodes

34 μm Carbon Fiber Electrodes

7 μm Carbon Fiber Electrode for In Vitro, Brain Slices

7014-L

34 μm Carbon Fiber Electrode for Rats (Wireless)7002-CFE

7 μm Carbon Fiber Electrode for Freely Moving In Vivo

7014-S

34 μm Carbon Fiber Electrode for Mice

7004-CFE

7 μm Carbon Fiber Electrode – No Connector

7015

All 34 μm CFEs are bought by cannula type. See page 2 for details.

Silver Wire + Pin for 7015

7016-SWP

Silver Wire + Pin + Connector for 7015

7016-SWPC

Reference Electrode
Ag/AgCl Reference ElectrodeAll 7 μm CFEs are bought by cannula type. See page 2 for details.

7065

7 μm Enzyme-Modified Carbon Fiber Electrodes For Detection of Glucose and Dopamine
GLUCOSE ENZYME-MODIFIED CARBON FIBER ELECTRODES(EM-CFEs) are used to monitor real-time, sub-second, changes inconcentration of multiple analytes using a single electrode. Glucose
oxidase is entrapped in a hydrogel matrix allowing for the simultaneous detection of non-electroactive species (glucose and DA) via
Fast Scan Cyclic Voltammetry (FSCV).

7 μm ENZYME-MODIFIED CFE
7 μm EM-CFE for In Vitro, Brain Slices

7017-L-Glucose

7 μm EM-CFE (Wireless) for Freely Moving In Vivo

7017-S-Glucose

7 μm EM-CFE – No Connector

7018-Glucose

Glucose Peak

GLUCOSE EM-CFE specifications
Electrode Diameter

7 µm

Active Area Length~ 100 µm

Glucose Linear Range

0.05 to 2 mM

Pulled Capillary Electrode (includes connector wire)

Dopamine PeakFor Use in Brain Slices or In Vivo

20

SYNCHRONIZED VIDEO

Synchronized video capability
Pinnacle’s VIDEO SYSTEM can record up to four simultaneous in vivo experiments on one computer. Our Sirenia® video software
licenses unlock the ability to synchronize video recordings ± one video frame up to 30 frames per second to simultaneous EEG, EMG
or biosensor data. This provides an accurate representation of an animal’s overt behavior in conjunction with its physiological response.
The video system consists of a base computer package and a camera package, including a synchronized video license which provides
everything you need to incorporate video capture into your research. Order it as an accessory to a new system or easily integrate it
into your current Pinnacle setup.

Dome Camera

Camera Options

Pinnacle’s dome camera mounts above the cage. Its built-in infrared
illumination adjusts to lighting conditions automatically, allowing video
recording in reduced lighting and complete darkness.

Synchronized Video System
Base Video Computer Package for Box/Dome
CameraBox Camera
9000-K1

Base Video Computer Package for USB
Camera9000-K1-USB

Includes a preconfigured computer, a high-definition monitor, a docking station foreasy data transfer, a keyboard, mouse and cables. Up to four cameras can be added
to a single video system. Cameras sold separately.

Camera Packages
Dome Camera with Integrated IR Source

9000-K9

Box Camera with Independent IR Source

9000-K10

USB Camera with Integrated IR Source

9000-K30

The box camera has improved optics and low-light performance comparedto the dome camera. It can be mounted above the cage, on the cage standor to a tripod for recording at lower angles. A separate automatic infrared
illumination source allows video capture in conditions of low light andcomplete darkness. An external IR source is included.

USB Camera
The low-latency, USB camera use a single cable for power and data transfer.
Similar to our dome camera, the USB camera has a built-in IR source that
automatically adapts to lighting conditions, ensuring high-quality video
recording even in low light and complete darkness. The camera can beconnected by plugging in via USB port or hub and does not require a video
capture card.

Synchronized video capability

All camera packages include a camera, IR source, mounting accessories, extension
cable and one Sirenia® license key.

ACQUIRE

Additional Products
Variable Focus Lens for Box Camera

9056-VF

Tripod for Box and USB Cameras

Pinnacle’s Sirenia® software uses frame-byframe timestamping to synchronize video
within +/- 1 video frame to simultaneous
EEG, EMG or biosensor data.

9059

Key features
Record in color or grayscale◊

Record in low light or complete darkness◊

Flexible file size management◊

Synchronize video with data recordings◊

Unrestricted video playback◊

Record from any angle (Box/USB)

Features

Dome

Box

USB

Lens

3.6 mm (internal)

4 mm (external)*

2.8–12 mm variable (external)

Max Resolution

704 x 480 pixels**

704 x 480 pixels**

640 x 480 pixels

Max Frame Rate

30 fps

30 fps

30 fps

Mount

Above

Above, Side, Tripod

Above, Side, Tripod

IR Source

Integrated

Independent

Integrated

Color/Grayscale

Both

Both

Both

Interface

AnalogAnalogUSB 2.1*Accepts standard C-mount lens **Capture Card Specific21

Analyze◊

A visual sync bar allows users to easily
match the displayed video frame withcorresponding data for an accurate visual
representation of animal activity.

ManageExtract segments from long recordings
for simplified data transfer or archival.
Plus, export video with associated data for
playback during presentations.

upgrade toenhanced illuminatorSee page 24 for details.

ANIMAL HOUSING

Animal housing
CIRCULAR CAGES and CAGE ACCESSORIES allow tethered rodents to have complete freedom of movement. All cages are madeof one-fourth inch clear acrylic and are suitable for use with most commercial cage washing equipment. Multiple sizes are available.
Circular cages are ideal for both wireless and tethered experiments. Cage accessories include items such as cage lids, stands andilluminators. Housing items can be purchased individually or in kits that include necessary components for an experimental set-up.
1 Cage lids allow for additional containment of active animals, canbe easily removed for cleaning and feeding. Standard cage lids aredesigned for tethered experiments. FILTER CAGE LIDS can beused for wireless experiments or when using the cage for long-term
housing.
2 Increase the height of our standard cages using cage extenders.

The extenders fit snugly on top of Pinnacle’s cages and add 4” (perextension) to cage height. Extenders can be stacked for additionalheight. Not included with the purchase of cage kit.

1

3 Pinnacle’s cages are ideal for tethered rodent research. The circular

design allows the animal complete freedom of movement aroundthe circumference of the cage. The open top makes cleaning andfeeding easy and accessible. For tethered set-ups, our circular cage
design prevents excessive slack and tension in the cable.

2

4 Pinnacle’s stands accommodate a single animal cage and mounting

3
4
5

plate. The split design is compatible with both mouse and rat cages
and all of Pinnacle’s tethered recording systems. A new option, aMONOPOD STAND, provides a simplified design with increased
access to the animal. Mounting plate sold separately.
5 A detachable food hopper can be added to any Pinnacle cage.

The stainless steel food hopper attaches to the inside of the cage,holds approximately 50 grams of food (~12 standard pellets) anddispenses one pellet at a time. It reduces disturbance to the animal
and requires little maintenance during long-term experiments. Add asecond food hopper to increase the amount of food available.

Additional Products

Cage Kits

14” Mounting Plate (35.5 cm)

8258-723

18” Mounting Plate (45.7 cm)

8426

10” Mouse Cage Set-up

9000-K20

Cage for mice (10” diameter, 8” tall [25.4 x 20.3 cm])

8228

Cage lid for mice

8265-M

10” Mouse Cage Extender8228-4ex
Monopod metal stand (adjustable to 20” tall [61 cm])

9009-MOM

12” Rat Cage Extender8238-4ex

Rubber Bumpers for Monopod Stand

9009-RB

8273-4ex

T-Handle hex key

8241-KY

14” Rat Cage ExtenderShaft collar

9009-SC

Water bottle for mice

8251-M

Mouse food hopper

9066-M

Cage Stands
Cage Stand (Adjustable to 24” tall [61 cm])

9009

Cage Stand (Adjustable to 36” tall [91 cm])

9009-RSD

Monopod Cage Stand (Adjustable to 20” tall [51 cm])

9009-MOM

Mounting Plate for Monopod Cage Stand

9009-PL

Filter Lids

10” Mouse Cage Set-up (Wireless)*

9000-K26

12” Rat Cage Set-up**

9000-K21

12” Rat Cage Set-up (Wireless)***

9000-K27
9000-K22

10” filter lid for mice

8265-M-F

14” Rat Cage Set-up****12” filter lid for rats

8265-R-F

14” filter lid for rats

8265-R14-F

Grid Filter Paper

8265-FP* Components of this kit are similar to above but includes filter lid accessories.
** Components of this kit are similar to above but sized for larger cage dimensions.
*** Components of kit are similar to above but includes filter lid accessories and is sized forlarger cage dimensions.
**** Components of this kit are similar to above but include 9009 stand and is sized for larger
cage dimensions.

22

ANIMAL HOUSING

Group housing
Pinnacle’s GROUP HOUSING SYSTEM is a modular, customizable social housing chamber for studies using up to four rats. The systemallows for a variety of experimental set-ups, including behavioral and physiological studies. It can also be used as a home cage for longterm experiments. The cage has easily interchangeable panels and an option to add video and RFID tracking.

MIX & MATCH
Choose any four walls to be added to your core cage set-up. Order additional

14panels to swap out for different experiments. Select behavioral and tracking
components to enhance your group housing system.

2
3

Panel Options
1

Blank panel with no additional features.

2

Operant panels include mountings for up to three fixtures compatible withthird -party products.

3

Door panel for easy access to the animals without removing the lid.

4

Food hopper and water bottle panel.

Behavioral & Tracking Components
TRACKING
Our MULTIPLE ANIMAL TRACKING SOFTWARE is used to track animal movement and analyze behavioral patterns based on speed, distance,
trajectory and animal position. The software can be combined with RFID to automatically resolve collision events and ensure positive identification ofanimals. Alternatively, RFID can be used aloe for zone-based animal identification.

BEHAVIOR AND OPERANT CONTROLS
Third-party operant devices can be integrated into the group housing system. Hardware can be connected through Pinnacle’s DIGITALINSTRUMENTATION INTERFACE and controlled through within the Sirenia® software platform. Sirenia FEEDBACK PRO software gives users
control over input rules and output device function.

LIGHTING
Use Pinnacle’s illuminators to calibrate and control cage lighting. Basic and enhanced illuminators provide optimal lighting conditions for video
recordings in a day or night setting and are ideal for studies that require strict lighting conditions.

Behavioral and Tracking Components
RFID System*
RFID module

9000-K25
9087

Video and Lighting Systems
Box camera with independent IR source

9000-K10-GH

IP camera with independent IR source

9000-K11-GH

Enhanced Illuminator

9000-K15

IR Source/Illuminator

9057

Core Cage Kit
Group Housing Core Cage
Cage strut (2)Sirenia® Feedback Pro software

9030

Sirenia® Multiple Animal Tracking software

9040

22” Cage

9000-K23

9000-K24

9088-169088-22

Angle bracket sides (2)9089-169089-22Top brace bar (2)9088-T16

9088-T22

Floor with sides

9092-169092-22

Lid

9091-169091-22

16” Cage22” Cage

Blank Panel

9083-169083-22

Door Panel

9083-D16

9083-D22

9000-K23-OP

9000-K24-OP

9083-O16

9083-O22

9084

9084

9000-K23-FW

9000-K24-FW

Wall Panel Kits

Premium Software Modules

16” Cage* All necessary cables are also included.

Operant Panel
Operant panel
Digital instrumentation interface

Food and Water Panel

23

ADDITIONAL PRODUCTS

supporting Products
Pinnacle’s neurophysiological systems implement a modular design strategy, allowing the easy addition of new measurement tools to
expand the range of experiments possible within a single recording setup. Our devices can also be integrated with third-party hardwarein order to add new functionality to existing systems. Several key products include electrical or optical stimulation, illuminators, lowtorque commutators, isolated potentiostats and devices to integrate third-party hardware.

stimulus controller kit
Add optical or electrical stimulation to Pinnacle recording systems
The addition of a Stimulus Controller module for either electrical or optical stimulation unlocks the ability to perform neuronal control
with simultaneous biopotential, neurotransmitter and behavioral recordings. Pinnacle’s Stimulus Controller module offers precise controlof electrical or optical stimulation frequency, duration and intensity for both continuous and discrete events. The stimulus controller
integrates directly with our 8400 4-channel systems and preamplifiers. It can also be used as a stand-alone device. The optogenetic
expansion kit is also sold with an accessory kit that includes surgical and testing equipment.

STIMULUS CONTROLLER
Hardware kits

Mice

Rats

Electrical Stimulator & Biopotential Kit

8400-K22

8400-K23

Optogenetics Expansion Kit

8400-K24

8400-K25

Electrical Stimulation Expansion Kit

8400-K26

8400-K27

Mice

Rats

8400-K28

8400-K29

All kits include cables and manuals.

OPTOGENETICS
Accessory Kit
Optogenetics
Optogenetics Friction Fit

8400-K30

Includes optogenetics-specific surgical and testing equipment.

Key features◊

Highly accurate clock ensures precision timing◊

Two TTL inputs configurable as hardware stimulus triggers◊

Gate and stimulus control simplify connection to stimulus isolators◊

Store and run two independent stimulus paradigms◊

Capable of controlling either electrical or optical stimulus◊

Hardware signaling to the data acquisition system ensures persample accuracy of stimulus events

Ir Source

Enhanced illuminator

Record nighttime video in animal enclosures

IR and visible light source with dawn/dusk transitions

Our

This

IR

sourcehasabuilt-inphotoresistor to automatically turn on

programmable

illuminator

controls

lighting and monitors temperature, humidity

IR lighting in darkness makes it ideal

and light intensity in or near the enclosure.

for long-term video recordings.

The device provides both IR and visible lightand can also simulate dawn/dusk transitions.

24

ADDITIONAL PRODUCTSCOMMUTATORSTTL PLUS

Low-torque electrical swivels

Synchronize external signals or third-party devices inSirenia

Pinnacle’s

low-torquemousecommutators

provideexcellent

freedomofmaintaining

while

connections, enabling the integration

constantof TTL signals to a wireless system or

movement
clean

and

The TTL+ adds four multipurpose I/O

electrical signals. Custom modifications are available for use withexpansion of TTL recording capabilities

third-party cabling and connection schemes. Rat commutators

in tethered systems. Each I/O connection can be configured as

are also available.

a 5V digital output, 5V digital input, or 10-bit analog input, withsampling rates of up to 2000Hz. Digital inputs can be configured

ANALOG ADAPTERS

to create annotations in electrophysiology recordings acquired byother Pinnacle devices, and digital outputs can be used to trigger

Use your own acquisition system with Pinnacle
hardwarePinnacle’s three- and four-channel
analog adapters allow researcherswith third-party data collection systems

external events based on real-time analysis of electrophysiology
events.

MASTER CLOCK
Synchronize timing between independent USB devices

to take advantage of the excellent
noise

reduction

providedby

The master clock provides centralized

ourultra-precise timing for the synchronization

commutators and head-mounted preamplifiers. These adaptersof multiple devices such as video andare compatible with both Pinnacle’s mouse or rat preamplifiers.

data interfaces, making it perfect for
optogenetics,

Analog Adapters can be powered by batteries or a power supply.
DX models are built with both power and battery supply options
integrated into a single device, and have factory-settable gain
options available for researchers that need additional signal
amplification.

ISOLATED WIRELESS POTENTIOSTAT
Hand-held isolated electrochemical measurements
The

wireless

two-channel

potentiostat

comes in standard or extended range
models and is suitable for a wide range ofamperometric systems, including lab-onchip and biosensors. Its isolated design
makes it uniquely suited for in-channel

behavioral

studiesandother protocols. The Master Clock works
seamlessly with our Sirenia® software offering < 1 ppm accuracy
from 0 to 70°C and three TTL I/O ports. It connects to the systemusing a standard USB cable.

ADDITIONAL Products
IR Source

9057

Enhanced Illuminator Kit*

9000-K15

Enhanced Illuminator

9057-EN

Lux, temperature and humidity cable

9057-LUX

Photoresistor cable

9057-PHO

Commutators
6-Pin mouse commutator

8204-723

9-Pin mouse commutator

8408

10-Pin mouse commutator

8481-Mand end-channel detection in capillaryelectrophoresis systems. Powered by astandard 9 V battery, the device uses Smart
Bluetooth® telemetry for reliable data transfer via a USB dongle
and is fully supported by Pinnacle’s Sirenia® software.

Analog Adapters
3-Channel analog adapter (Dual-powered option)*

8242-DX-K

4-Channel analog adapter (Dual-powered option)*

8442-DX-K

Isolated Wireless Potentiostats
Isolated wireless potentiostat*

Visit our website for additional products
to support your researchwww.pinnaclet.com

25

9000-K7

Extended range wireless potentiostat*

9000-K7-ER

TTL Plus

9085-N-K

Master Clock*

9000-K8*Cables, software and manuals are included in kit.

SOFTWARE

Sirenia® Suite Overview
Pinnacle’s SIRENIA® SOFTWARE provides powerful tools for preclinical research. This modular platform consists of a free acquisition
package, which is included with our hardware, and premium modules that can be added at any time.

FR

E

E

Sirenia® ACQUISITION

Key features

SIRENIA® ACQUISITION provides a single platform forrecording data from any Pinnacle hardware system, except◊

Record from Pinnacle devices
on a single platform◊

Save and load device
configurations

data consolidation, user-configurable settings and multiple export◊

Annotate in real-time or review◊

Schedule recording

options. In addition, the download includes basic analysis modules◊

Export to multiple formats◊

TTL functionality

FSCV. The software features data stream synchronization,

for biosensors, sleep and seizure recordings. Sirenia® delivers allin-one software for data acquisition and review.

Premium Modules
PREMIUM MODULES can be plugged seamlessly into the free core acquisition package. Learn more about these time-saving modules
on pages 27-28.

Sleep pro

Seizure pro

Score mouse and rat data in 75 percent
less time using automated tools, suchas cluster and threshold scoring.

X-y tracking

SLEEP

X-Y
TRACKING

Precisely track animal movement
and quickly analyze movement
and behavioral patterns.

Multiple Animal tracking

Feedback Pro
FEEDBACKSYNCHRONIZEDVIDEO

Use biopotential inputs andother rules to automate events,
such as sleep deprivationand electrical/optogenetic
stimulation.

Synchronized Video

Simultaneously record and track multiple
animals within a single enclosure andanalyze behavioral patterns.

Synchronize recorded video automaticallywith EEG/EMG waveforms and biosensor
activity. See page 21 for details.

Premium Software Modules
Sirenia Sleep Pro

9035-K-E

Sirenia® Seizure Pro

9037-K-E

Sirenia® Synchronized VideoSEIZURE

Sirenia®
Acquisition

MULTIPLE ANIMALTRACKING®

Quickly identify and analyze events inyour data using line length and power
characteristics of user-defined seizures.

zzz

9021

Sirenia® Feedback Pro

9030-K-E

Sirenia® X-Y Tracking

9039-K-E

Sirenia® Multiple Animal Tracking

9040-K-E

Contact a Pinnacle representative
to request a free trial:
sales@pinnaclet.com

30-DAYTRIAL®

All electronic software packages include a Sirenia software manual and license
key.

26

SOFTWARE

Premium Sirenia® modules
Pinnacle’s premium modules offer enhanced features for data collection and analysis. Data recorded with Pinnacle software, as wellas third-party EDF files, can be imported into all analysis software platforms. Premium software packages can be installed on multiple
computers but are limited to one computer running the program at a time. Purchase includes one year of free upgrades.

Sirenia® Sleep Pro

Sirenia® Seizure Pro

SLEEP PRO provides two automated methods for scoring sleep

SEIZURE PRO is designed for efficient seizure event analysis,

data—cluster and threshold scoring—as well as a manual scoring

allowing users to quickly identify and examine specific seizure

option. Custom scoring and analysis are also available. Multiple

events. Users can construct a seizure database by manually

methods can be combined to quickly and accurately score both

selecting events or establishing chosen parameters to findmouse and rat files. Epoch lengths are user-configurable andseizures. Once added, statistics such as amplitude, average

numerous scoring sessions can be created for the same file.

duration, temporal intervals between seizures, frequency power,

Powerful analysis tools such as sleep stage/sleep bout analysis

line length, and seizure type can be generated. Additional tools,

and user score comparison make reviewing and exporting datalike coherence and spike counter, are available with simple

easy.

parameter-setting options.

Cluster Scoring

Threshold Scoring

Key features◊

Multiple scoring tools◊

Peak frequency analysis◊

Automated seizure identification◊

Seizure statistics◊

Hypnograms◊

Coherence plots◊

Spectral plot/Heat maps◊

Coherence plots◊

Spectral plots/Heat maps◊

Power analysis◊

Power analysis◊

Spike Counter

Accuracy of seizure detection

A combination of cluster, threshold and manual scoring tools were usedby four experienced and novice scorers to separately score three different
mouse data files. All files were compared to expert hand-scored data
files. The overall average agreement of the four scorers for all the files ascompared to the expert is shown below.

Seizure detection was performed using RMS power and line length
separately on five individual mouse data files. All files were compared toan expert hand-scorer’s files. Agreement of the two detection methods
as compared to the expert is shown below.

Sleep State

Accuracy of sleep detection

Wake

89.7%

NREM

94.7%

REM

80.2%

0

27

Key features

20

40

60

80

# of Seizure
Events Marked

Accuracy vs.
Expert Scorer# of False
Positives

Expert Scorer

21

N/A

N/A

RMS Power

23

100%

2

Line Length

21

100%

0

100

Percentage Correct Compared to Expert

Data courtesy of Drs. Philip Haydon and Jerome Clasadonte (Expert Scorer)
Tufts University School of Medicine, Department of NeuroscienceSOFTWARE

Sirenia® Feedback Pro
SIRENIA® FEEDBACK PRO software enables users to create rule sets based on baseline data, thresholds and power analysis toinitiate stimuli in a variety of sleep, seizure, optogenetics, and behavioral studies. When used in conjunction with Pinnacle EEG/EMG
recording devices, real-time signals can be analyzed by the software based on user-programmed rules. Additionally, our software
allows researchers to connect and synchronize the settings of multiple units. Other features include TTL controls for third-party systems,
interface for third-party modular behavioral components, such as levers, lights, dispensers, single and parallel state machines, flexible
inputs including entry into zones defined by videoor RFID and support for group-housed animals.

Key features◊

Compatible with Pinnacle’s optogenetics andelectrical stimulation system◊

Coordinate and schedule TTL signals◊

Control stimuli based on physiologicalresponses

Sirenia® X-Y TRACKING
SIRENIA® X-Y TRACKING enables users to accurately detectand analyze animal movement within a cage. Users can track
locomotor behavior in real-time or in previously recorded video
data. In addition, the software can be calibrated to different cage
types and is compatible with tethered and wireless animals,
making X-Y Tracking applicable to a variety of experimental
paradigms including sleep/wake activity.

Key features◊

Real-time tracking◊

Distance and velocity traveled◊

Heat maps◊

User-defined regions◊

Motion trajectory analysis◊

Quadrant analysis

Above: A mouse’s motion trajectory, an X-Yheat map, and distance and velocity data aredisplayed on the right side of the screen. Left:
An X-Y heat map is displayed over the video
image.

Sirenia® Multiple Animal TRACKING
Simultaneously record and track multiple animals within a single
cage using SIRENIA® MULTIPLE ANIMAL TRACKING. Users
have the ability to monitor and analyze animal movement basedon speed, distance, trajectory, and animal position. RFID modules
can be added to automatically resolve collision events.

Key features◊

Heat maps◊

Motion trajectory plots◊

Integrated RFID tracking◊

Automatically resolve collision events28

Learn more
Pinnacle’s products are used daily to advance research at academic institutions, research hospitals, government laboratories, contract researchorganizations and pharmaceutical companies across the world. Learn more about how current customers are using our products by visiting the Info
Center on our website at www.pinnaclet.com/info-center.html.

Publications

Posters

Many Pinnacle customers regularly publish their

Missed a conference? Visit us online toscientific work in peer-reviewed journals. This

review posters presented by Pinnacle andspace is dedicated to sharing our customers’

our customers at SfN Neuroscience, AES,

research with other users of Pinnacle products

FENS and other major conferences. If you’ve

and systems. Visit our website at www.pinnaclet.

presented a poster recently using Pinnacle

com/publications.html for a complete list of

products, let us know! We may be able to post it

publications.

online to share with other researchers.

Conferences

Distributors

Pinnacle Technology regularly attends scientific

Pinnacle

conferences and meetings hosted both in theour products available to an international

United States and internationally. Please stop bycustomer base by teaming with a growing list

our booth at upcoming conferences to chat withof distributors from across the globe. Visit oura representative about how our cutting-edge tools

website at www.pinnaclet.com/distributors.

can improve and simplify your research. Visit ourhtml for an updated list.

websiteatwww.pinnaclet.com/conferences.

html for a complete list of events.

Technology

is

proud

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make