Anti-APP-C31 (C-terminal fragment of the caspase 3-cleaved APP) antibody, rabbit
serum (ACT1)
Product code74-108Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityRabbit antiserum

ImmunogenSynthetic peptide corresponding to the N-terminus of caspase 3-generated APP
C-terminal 31 amino acids (aa 665-670 of human APP695).

IsotypeRabbit IgG

Reactivity

Reactive to human, mouse and rat. Specific to the N-terminal end of the caspase
3-generated APP-C31.

Special notes

N/A

Application

1. Western blotting (dilution: 1/3,000-1/1,000)
2. Immunocytechemistry (dilution: 1/1,000-1/500)
3. ELISA
These applications were confirmed in the laboratory of Prof. K, Yoshikawa of
Osaka University. (ref.3).

Background

The Alzheimer Amyloid Precursor Protein (APP) is a transmembrane protein
whose abnormal processing is associated with the pathogenesis of Alzheimer’s
disease. APP695 lacking the protease inhibitor domain is the predominant formin neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino(N)-terminal fragment that lacks the carboxyl C-terminal 31-residues(APP C31) and the 31-residues C-terminal fragment (APP-C31). Bothfragments might be potent inducers of neuronal apoptosis. An antibody (namedACT1) against the N-terminus of caspase 3-generated APP C-terminal 31 aa of
human APP695 (APP-C31) was raised in rabbit.

Data Link

UniProtKB P05067

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 74-108 Anti-APP-C31 (C-terminal fragment of the caspase 3-cleaved APP) antibody,rabbit serum (ACT1)

Fig.1 Immunocytochemistry for APP-C31: Specific reactivity of the antibody ACT1 with C-terminal
fragment of the caspase 3-cleaved APP.
NT2 neurons were fixed 72 h after infection with adenovirus vector expressing Amyloid Precursor
Protein.
Left image: stained for the N-terminus of APP with P2-1, mouse monoclonal antibody specific for Nterminus of APP (non BioAcademia).
Center image: stained for chromosomal DNA (Hoechst).
Right image: stained for C-terminus of the caspase 3 cleaved APP with anti-APP-C31 antibody (ACT1).
Most of APP-accumulating neurons with shrunken and fragmented nuclei contain ACT1immunoreactivity (arrows), but non-neuronal cells are hardly labeled with ACT1 (arrowheads).
References: This antibody was used in ref.3.
1. Kang HG et al. (1987) “The precursor of Alzheimer’s disease amyloid A4 protein resembles a cellsurface receptor.” Nature 325: 33-736 PMID: 2881207
2. Selkoe DJ (1994) “Normal and abnormal biology of the beta-amyloid precursor protein.” Annu.

Rev. Neurosci. 17: 489-517 PMID: 8210185
3. Nishimura I et al. (2002) “Cell death induced by a caspase-cleaved transmembrane fragment ofthe Alzheimer amyloid precursor protein.” Cell Death Differ. 9: 199-208 PMID: 11840170
4. Nishimura I et al. (2003) “Upregulation and antiapoptotic role of endogenous Alzheimer amyloidprecursor protein in dorsal root ganglion neurons.” Exp. Cell Res. 286: 241-251 PMID: 12749853
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2 / 2

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/