PROCESSSkillPak™ 1 mL Instruction Manual for TOYOPEARL®,
TSKgel® and Ca++Pure-HA™ Process MediaIntroduction
SkillPak 1 mL columns (7 mm ID × 2.5 cm) are designed for resin screening,
method development and sample concentration for biomolecules. Thesecolumns are pre-packed with TOYOPEARL, TSKgel or Ca++Pure-HA process
chromatography media and are designed to be operated with commonlyused low or medium pressure liquid chromatography systems.
Product Description

Stop plug

Hardware Materials

The hardware of SkillPak 1 mL columns iscomposed of bio-compatible polymers,mainly polypropylene. Table 1 lists
properties and operation specificationsof the SkillPak 1 mL columns.

Column tube

Instructions for Use
Installation

Snap tab

SkillPak 1 mL columns are ready to use upon receipt. Snap off end at outletof column and screw directly into chromatography system. Connect inlet ofcolumn to instrument using 10-32 fitting. Finger-tight is recommended.
Equilibration

Before the first sample injection, the column should be equilibrated with at least
10 CV of the respective loading buffer. See Table 1 for flow rate and operatingpressure. Slowly increase flow until it reaches the desired running flow rate.
Do not exceed the recommended maximum flow rate.

Table 1. Specifications of SkillPak 1 mL columns
Column dimension

7 mm ID × 2.5 cm bed height

Volume1 mL

Maximum flow rate

4 mL/min (600 cm/hr)

Maximum operating pressure

0.3 MPa

Connections

Standard fittings (10-32 for 1/16 inch capillary)

Shipping buffer

20% ethanol for TOYOPEARL and TSKgel [with theexception of 0.5 mol/L sodium citrate with 20%ethanol for TSKgel SP-5PW (20) and SuperQ-5PW (20)],20 mmol/L phosphate with 20% ethanol for Ca++Pure-HA

Buffer composition and gradient design is dependent on the resin type and resin
chemistry. Please refer to the TOYOPEARL, TSKgel or Ca++Pure-HA productdocumentation for more information about recommended mobile phases.
Usage

SkillPak 1 mL columns are intended for one time use/one series of injections whileremaining connected to the system. The columns are not designed to be repacked.
Tosoh Corporation
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Tel: +81-3-5427-5180
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email: info.tbg@tosoh.com
Tosoh Asia Pte. Ltd.
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Tel: +65-6226-5106
email: info.tsas@tosoh.com

Tosoh Bioscience LLC
3604 Horizon Drive, Suite 100
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Tel: +1-800-366-4875
email: info.tbl@tosoh.com
Tosoh Bioscience Shanghai Co., Ltd.
Room 1001, Innov Tower, Block A
1801 Hong Mei Road,Xu Hui District, Shanghai 200233, China
Tel: +86-21-3461-0856email: info.tbs@tosoh.com.cn
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Mumbai - 400 063, India
Tel: +91-22-614-85200
email: contact@tosohindia.com

www.tosohbioscience.com
Tosoh Bioscience, TSKgel and TOYOPEARL are registered trademarks of Tosoh Corporation.
Ca++Pure-HA is a registered trademark of Tosoh Bioscience LLC in the USA, EU, India, and Japan.
SkillPak is a registered trademark of Tosoh Bioscience LLC in the USA.

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