Anti-Collectin-12 / CL-P1 antibody, mouse monoclonal (53)Product code72-041Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Recombinant human collectin-12 (amino acids 590 to 742) corresponding to thecarbohydrate recognition domain (CRD) expressed in Escherichia coli.

Isotypemouse IgG1κ

Reactivity

Human, mouse, rat and chinese hamster

Special notes

N/AApplication

1. Western blot(1/500~1/1,000 )2. Immunofluorescent staining (1/500)
3.ELISA (assay dependent)

Background

Collectins are characterized by a collagen-like sequence and a carbohydrate
recognition domain (CRD) and are members of the vertebrate C-type lectinsuperfamily. Collectins play an important role in the innate immune system.
The collectin-12 (Collectin Placenta 1;CL-P1) is detected in placenta andumbilical vein, and expressed in vascular endothelial cells in human. CL-P1 hasan open reading frame of 2226 base pairs encoding 742 amino acids. CL-P1 has
an approximate molecular mass of 140 kDa in CL-P1-cDNA-transfected CHO(Chinese hamster ovary) cells and placental membrane extracts.

Data Link

UniProKB Q5KU26 (COL12_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 72-041 Anti-Collectin-12 / CL-P1 antibody, mouse monoclonal (53)Fig.1. Identification of protein CL-P1 by Western blotting
Supernatant of CHO cells expressing human recombinant CL-P1 (1µg/ml) was separated on SDSPAGE. The monoclonal antibody was used at 1/500 dilution.
The CL-P1 protein migrates at 140 kDa position.

Fig.2. Detection of CL-P1 protein transfected in CHO cells by immunofluorescence staining
A: CHO cells expressing human recombinant CL-P1.
B: CHO cells (mock infected cells).
The antibody was used at 1/500 dilution. The FITC-conjugate rabbit anti-mouse IgG (x4000) wasadded. Nucleus (DNA) was stained with DAPI.

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Fig.3. Titration of recombinant CL-P1 protein transfected in CHO cells by indirect ELISA using
monoclonal antibody (53).
The indicated amounts of recombinant CL-P1 protein expressed in CHO cells was coated onto thewells of the ELISA plate. After blocking with 5% skim milk, monoclonal antibody at the 1/1000
dilution was added to the each well. HRP-conjugate goat anti-mouse IgG (100μl, x4000 dilution) wasadded. As substrate, orthophenylenediamine was used. Optical density (OD) measured at 490nm.
Related product72-040 Anti-Collectin 11 / CL-K1 antibody , mouse monoclonal (821)
References: This antibody was used and cited in the following publications.
1.Ohtani K. et al. The membrane-type collectin CL-P1 is a scavenger receptor on vascular endothelialcells. J Biol Chem. 2001 Nov 23;276(47):44222-8. PMID：11564734 WB, IF, ELISA: human
2. Koide T et al. Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47binding structural motif in collagens and related proteins. J Biol Chem. 2006 Apr 21;281(16):1117785. PMID: 16484215. WB: human
3.Jang S. et al. Scavenger receptor collectin placenta 1 (CL-P1) predominantly mediates zymosan
phagocytosis by human vascular endothelial cells. J Biol Chem. 2009 Feb 6;284(6):3956-65PMID:19073604 WB, IF: human
4. Koyama S et al. The induction of human CL-P1 expression in hypoxia/reoxygenation culture
condition and rat CL-P1 after ischemic/reperfusion treatment. Biochim Biophys Acta. 2011
Sep;1810(9):836-42. PMID: 21723916. WB:human
5.Jang S et al. Scavenger receptor CL-P1 mediates endocytosis by associating with AP-2μ2. Biochim
Biophys Acta. 2014 Nov;1840(11):3226-37. PMID: 25109811. WB, IF: human

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