TECHNICALDATASHEETLight Producing Microorganisms

Caution: For Research Use. This product is intended for animal research only and not for use in humans. Not for human or animal therapeutic ordiagnostic use.

Pseudomonas aeruginosa
P. aeruginosa ATCC 19660 (Xen5)
Product No.: 119228
Material Provided: 1 Agar Plate
Storage Conditions: -80°C
In vitro Characteristics
Genetic Characteristics
Pseudomonas aeruginosa Xen 5 was derived fromthe parental strain P. aeruginosa ATCC 19660, amucoid clinical strain isolated from humansepticemia in Lima, Peru. P. aeruginosa Xen 5
was engineered through conjugation andtransposition of plasmid carrying transposon Tn5
luxCDABE. P. aeruginosa Xen5 possesses asingle stable copy of the P. luminescens lux
operon on the bacterial chromosome.
Growth Characteristics
P. aeruginosa Xen 5 grows well in various media
including Luria Bertani (LB), Nutrient Broth (NB)
and Brain Heart Infusion (BHI) without antibiotic
selection at 37ºC under ambient aeration.
Alternatively, P. Aeruginosa Xen 5 may be grown
selectively in medium/agar containing 60 μg/ml
tetracycline to prevent contamination.
Colonial Morphology
On agar plates, P. aeruginosa Xen 5 appears aslarge (3-5mm), yellow-green, irregularly round,mucoid colonies with butyrous centers after 24hours incubation at 37°C.

119228-Xen05-Rev-1

Growth Curve
P. aeruginosa Xen 5 displays peak bioluminescence during log-phase growth, which can beachieved after 1.5 hours of subculture in LB brothat 37ºC with aeration at 200rpm. An absorbancemeasurement at 600 nm (against a LB blank) of
8
1.0 is roughly equivalent to 7.5x10 cfu/ml of P.
aeruginosa Xen 5.

Biochemical Profile
A biochemical profile was obtained for P.
aeruginosa Xen 5 using the api 20NE system
available from bioMérieux.
Assimilation

NCCLS Macrodilution MIC/MBC

Other Tests

GlucoseArabinose
MannoseMannitolN-acetylglucosamine
MaltoseGluconate++Caprate+Adipate+Malate
Citrate++Phenyl-acetate-++Nitrate Reduction
Indole Production
Glucose Ferment
Argenine Dihydrolase
Ureaseβ-glucosidase
Protease (gelatin)
β-galactosidase
OxidaseMIC and MBC Data
MIC and MBC were determined using themacro-dilution methods specified in theNCCLS Approved Standard M7-A5.

NO2+++++

Antibiotic

MIC (µg/mL)

MBC (µg/mL)

Ceftriaxone

31.25

125

Gentamicin

1.0

1.0

Tetracycline>125>125References
1. Infection & Immunity, Feb. 2003, Vol. 71, No. 2, 882-890
2. Infection & Immunity, July 2005, pp. 3878-3887
3. Journal of Immunology, 2004, 172: 1801-1808
4. Lasers in Surgery and Medicine 2007, 39:59–66
5. Blood, 15 December 2002, Vol. 100, No. 13, 4660-4667
6. Biomaterials. 2006 Aug;27 (22):4157-64
7. Methods in Molecular Biology 2008 Vol. 431, 225-239

Product Information
Warranty

Antibiotic Susceptibility
Disk Diffusion Data: Disk diffusion tests wereperformed according to methods outlined in theNCCLS Approved Standard M2-A7.

PerkinElmer warrants that cells will be viable
upon shipment from PerkinElmer for aperiod of thirty days, provided they havebeen properly stored and handled during thisperiod.

Kirby-Bauer Disk Diffusion Test
Sensitive to:Resistant to:Carbenicillin 100
Chloramphenicol 30(intermediate)
Tetracycline 30
Trimethoprim/sulfamethoxazole

Disclaimers
This product is sold for in vivo animal
research use only and is not for use in anydiagnostic procedures. Excluding purchasesby authorized PerkinElmer distributors, thisproduct is sold for use by the original
purchaser and is not for resale.

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119228-Xen05-Rev-1