13

Packing Materials

Packing materials for preparative separation -------124-125
Packing materials for HPLC and flash/open
chromatography ---------------------------------------- 126
Ordering Information------------------------------------ 127
Scale up to preparative separation ---------------------- 128
YMC-Triart ------------------------------------------129-131
YMC＊GEL HG------------------------------------------- 132
CHIRAL ART--------------------------------------------- 133
BioPro IEX SmartSep Q/S and BioPro IEX Q/S ----------- 134

13

Packing Materials
Packing materials

Packing materials for preparative separation
YMC offers packing materials for various purposes: HPLC, flash/open chromatography, chiral separation, and bioprocess
chromatography.
Our packing materials with high durability and high loading capacity are effective for not only HPLC but supercritical fluid
chromatography (SFC) and simulated moving bed (SMB) chromatography, and enable highly efficient and cost effective in various
fields such as pharmaceuticals and foods.

Abundant product lineup for wide separation range～ Enabling selection of optimum packing materials for the target substances ～YMC offers various products, such as HPLC packing including organic/inorganic hybrid silica based materials, ion exchange media forbioseparation, and chiral separation packing materials. Selecting the suitable separation mode and phase of the material can achieveeffective purification of small molecules, biologics (including oligonucleotides, peptides, and proteins), and chiral compounds.

Synthetic chemicals

NP

RP

SIL, CN
Diol, NH2
etc.

C18

Natural products

C8
C4

PeptidesIEXTMS
Phenyl
CN

Nucleic acids

SEC

CEX

Diol

AEX

etc.

etc.

etc.

Antibodies, ProteinsC H I RA L
Polysaccharide
derivatives

Chiral compounds

etc.

Production capacity/quality control
YMC can offer packing materials on the scale of 100 tons/year. YMC packing materials achieve excellent batch-to-batch reproducibilityby thorough quality control, so those are highly valued in various fields, including pharmaceuticals, foods, and chemicals, all over theworld. DMF (Drug Master File) registration indicates the high level of reliability of YMC products.

Excellent batch-to-batch reproducibility
The reproducibility of retention time among 10 batches of packing materials

Packing Materialst R Butyl benzoate (min)

25
20
15

Column: YMC＊GEL ODS-A-HG (10 µm, 120 Å)
250 X 6.0 mmI.D.
Eluent: acetonitrile/water (60/40)
Flow rate: 1.7 mL/min
Temperature : 40°C
Detection: UV at 270 nm
Sample: n-butyl benzoate

10
5
0
1

2

3

4

5

6

Batch Number

124

7

8

9

10

for HPLC and flash/open chromatography
YMC offers packing materials based on organic/inorganic
hybrid silica (YMC-Triart) and silica gel. YMC packing materials
with various phases and particle sizes meet any purpose andrequirement. It is effective also in semi-preparative separation
as well as industrial separation using axial compression
column.
YMC offers dynamic axial compression columns DAD of
automatic self-packing type and preparative HPLC system
K-Prep adequate for the packing materials.

DAD

K-Prepfor Chiral separation
CHIRAL ART are HPLC packing materials immobilized/coated with polysaccharide derivatives as chiral selectors, and are suitable for the
separation of a wide range of chiral compounds. CHIRAL ART packing materials have excellent mechanical strength and chemical stability
that can provide excellent peak shape without tailing. It enables to smooth and easy scale up from analytical to preparative purification.
CHIRAL ART are effective for supercritical fluid chromatography (SFC) and simulated moving bed (SMB) as well as HPLC, and achieve highefficiency and low-cost purification.

for Bioprocess chromatography＊ See chapter 15 (pp.151-) for details of preparative systems

Packing Materials

BioPro IEX media are adequate for the biopharmaceuticals
and protein purification. BioPro IEX media, which are basedon hydrophilic polymer with low nonspecific adsorption, aredesigned for the capture step to polishing step of proteinsand nucleotides. High dynamic binding capacity (DBC) andhigh recovery allow fast purification process at a large scale.
They offer high productivity on industrial purification of
peptides, proteins, and nucleotides including
biopharmaceuticals such as antibodies.
Various types of screening kits offer significant advantage andefficiency in media screening and purification method
development.
YMC offers biochromatography systems and columns.
YMC Pilot

BioStream

125

13

Packing Materials
Packing materials

Packing materials for HPLC and flash/open
chromatography
Specifications
Characteristics

Particle size(µm)

Pore size(Å)

UsablepH range

Page

Triart C18

Suitable as a first choice ODS packing with excellent durability

3, 5

120

1-12

49-51, 127

Triart Prep C18-S

Preparative ODS packing allows the effective cleaning of the gelwith alkaline solution

10, 15, 20

120

2-10

127, 129-131

Triart C8

Effective for fast separation of compounds with low polarity or
for separation of isomers

3, 5

120

1-12

53, 127

Triart Prep C8-S

Preparative C8 packing allows the effective cleaning of the gelwith alkaline solution

10, 15, 20

200

2-10

127, 129-131

Triart SIL
Triart Prep SIL

Organic/inorganic hybrid silica based packing material

3, 5, 10, 15, 20

120, 200—

127, 129-131

ODS-A
ODS-A-HG

Currently in use worldwide
ODS with wide pore size available, useful for separation ofproteins and peptides

3, 5, 75, 150
120, 200, 300

2-7.5

ODS-AM

Outstanding batch-to-batch reproducibility

120

2-7.5

120, 200, 300

2-7.5120, 200, 300

2-7.5

Product

Packing Materials
126

ODS-AQODS-AQ-HG

Superior separation of hydrophilic compounds

C8
C8-HG

Useful for separation of relatively highly hydrophobic
compounds, useful for separation of proteins and peptides

C4
C4-HG

C4 with wide pore size available, useful for separation ofproteins and peptides

10, 15, 20, 50
3, 5
3, 5
10, 15, 20, 50
3, 5
10, 15, 20, 50
3, 5
10, 15, 20, 50
3, 5

TMS
TMS-HG

Allowing rapid elution compared to other packing materials forretention based on hydrophobic interaction

10, 15, 20, 50

Ph (Phenyl)
Ph-HG

The π electron interaction gives a separation selectivity different
from ODS

10, 15, 20, 50

CN
CN-HG

The medium polarity of the bonded phase allows selectablenormal-phase and reversed-phase separation modes

YMCbasic

Superior separation of basic compounds and peptides

Omega

Superior separation of omega-fatty acids

SIL
SIL-HG

Fully porous silica gel packing material, popular among normalphase products

Diol
Diol-HG

Useful for gel filtration or normal-phase applications

NH2
NH2-HG

Chemically bonded with aminopropyl groups

CHIRAL ART

Packing material with polysaccharide derivatives chiral selector

3, 5
3, 5

82, 127

120, 200, 300

2-7.5120, 200, 300

2-7.5120, 200, 300

2-7.5

127, 132
82, 12783, 127
127, 132
84, 127132
84, 127132
85, 127132
85, 127132
86, 127

120, 200, 300

2-7.5

3, 5, 10

200

2-7.5

87

10, 20, 50

—

2-7.5

—

60, 120, 200, 300

2-7.5

60, 120, 200, 300

2-7.5

10, 15, 20, 50

3, 5, 75, 150
10, 15, 20, 50
3, 5
10, 15, 20, 50
5
10, 15, 20, 50
3, 5, 10, 20

120, 200, 300

2-7.5

—

2-9

132

96, 127
127, 13234-35
132100, 127132
62-66, 133

Ordering Information –Packing materials–
High resolution packing materials
Packing material

Particle size
(μm)
3
5
3
5
3
5
3
5
5
3
5
3
5
5
5
5
5
5
5
5
5
5
5
5

Triart C18
Triart C8
Triart SIL
ODS-AODS-AM
ODS-AQ
C8
C4
TMS
Ph
CN
SIL
NH2

Pore size
(Å)
120
120
120
120
300
120
120
120
300
120
300
120
120
120
300
60
120
120

Product number
TA12S03
TA12S05
TO12S03
TO12S05
TS12S03
TS12S05
AA12S03
AA12S05
AA30S05
AM12S03
AM12S05
AQ12S03
AQ12S05
OC12S05
OC30S05
BU12S05
BU30S05
TM12S05
PH12S05
CN12S05
CN30S05
SL06S05
SL12S05
NH12S05

Bulk packing materials
Packing material

Triart Prep C18-S

Triart Prep C8-S

Triart Prep SIL

ODS-A-HG

ODS-AQ-HG

10
15
20
10
15
20
10
15
20
10
15
20
50
10
15
20
50
10
15
20
50

Pore size
(Å)
120

200

120

120

120

120

Product number
TAS12S11
TAS12S16
TAS12S21
TOS20S11
TOS20S16
TOS20S21
TSS12S11
TSS12S16
TSS12S21
AAG12S11
AAG12S16
AAG12S21
AAG12S50
AQG12S11
AQG12S16
AQG12S21
AQG12S50
SLG12S11
SLG12S16
SLG12S21
SLG12S50

Packing materials for open column chromatography
Packing material
ODS-A
SIL

Particle size
(μm)
75
150
75
150

Pore size
(Å)
120
120

Product number

Packing Materials

SIL-HG

Particle size(μm)

AA12S75
AA12SA5SL12S75
SL12SA5

127

13

Packing Materials
Packing materials

Scale up to preparative separation
To establish a preparative-scale separation and purification method, separation conditions are first developed in analytical scale, thenshifted to preparative scale. For this scale-up, particle size, column inner diameter and length are selected based on sample load andspecifications of a purification system to be used. Then, further studies are conducted to optimize the separation conditions and load
for the selected particle size. (See pp.108-109 for details of optimization method for isolation/purification)
YMC offers packing materials for a wide range of applications from laboratory scale to plants scale. Furthermore, YMC’s solid
foundation of knowledge and resources help it purpose the most suitable preparative columns such as dynamic axial compression
columns, and preparative systems as well as contract services for method development/optimization and/or execution of preparative
purification.

Purification of corosolic acid from plant extract
Analysis

5 μm, 120 Å, 250 X 4.6 mmI.D., 1.0 mL/min

10 μm, 120 Å, 250 X 4.6 mmI.D., 1.0 mL/min

Select particle size 10 μm,
considered cost and columnpressure

Corosolic Acid

0

5

10

15

20

25

min

Requirement purity ≧ 95%
Deteriorate separation of
impurities
0

5

10

15

20

25

min

Separation improvement tolower flow rate

10 μm, 120 Å, 250 X 4.6 mmI.D., 0.5 mL/min
Optimization sample loading

Perform the preparative
separation in the maximum
load
0

5

10

15

20

25

30

35

min

Purification

Packing Materials

10 μm, 120 Å, 250 X 50 mmI.D.
60 mL/min, 94 mg load
ColumnEluent: ODS-AQ: methanol/water/TFA(85/15/0.05)
Temperature : ambient
Detection: UV at 210 nm

Purity: 98.1%
0

128

20

40

min

Organic/inorganic hybrid silica packing materials

YMC-Triart
Excellent mechanical stability
Excellent chemical durability and compatible with alkaline solution
Outstanding cost-effectivenessParticle size: 3, 5, 10, 15, 20 μm

Applicable from laboratory scale purification to industrial scale
Triart, YMC-Triart and YMC-Triart Prep, are organic/inorganic hybrid silica packing materials for preparative separation.
Triart’s excellent durability allows the effective cleaning of the gel with alkaline solution. It provides excellent mechanical stability, andcan be packed into a column repeatedly. Longer lifetime of Triart greatly contributes to reduction of production cost.

Specifications
Triart SIL

Triart C18

Particle size (μm)

Triart C8

Triart Prep SIL

3, 5

Pore size (Å)—

Usable pH range—

Triart Prep C8-S

10, 15, 20

120

Carbon content (%)

Triart Prep C18-S

20

17
1-12

120, 200

120

200

—

20

13

—

2-10 for regular use (-12 for alkaline CIP)

Versatile hybrid based material
Triart is based on novel organic/inorganic hybrid particles. The particle combines high mechanicalstability and high efficiency derived from silica based packing material, and high chemical stability
derived from polymer based packing material. The granulation process utilizing microreactor
technology enables continuous and highly controlled production of hybrid particles. The particle
has uniform pore size distribution and smooth surface as well as uniform particle size. This feature
greatly contributes to excellent peak shape and separation reproducibility.

Excellent mechanical stability
SEM images

Change in column pressure

15

Triart Prep C8-S
(10 μm, 200 Å)Conventional silica based C8(10 μm, 200 Å)

n=1

n=1

n=10

n=10

10

5

0

1

2

3

4

5
6
7
Packing number

8

Conditions of column packing
Column size : 100 X 50 mmI.D., Packing pressure : 6.5 MPa
Conditions of pressure measurement
Eluent : methanol/water (85/15), Flow rate : 50 mL/min

9

10

11

Packing Materials

Pressure (MPa/m)

Triart Prep C8-S (10 μm, 200 Å)Conventional silica based C8 (10 μm, 200 Å)

Triart Prep material still remained initial state after morethan 10 times of repacking. On the other hand, conventional
silica showed pressure increase or crush of particles.
Triart Prep with its high mechanical stability enables longer
column lifetime, and this feature provides reduction of
purification cost.

129

13

Packing Materials
Easy scale up from analytical to preparative
1. Oxytocin(MW 1,007)
2. Met-Enkephalin (MW 574)
3. Leu-Enkephalin (MW 556)
4. Neurotensin(MW 1,673)
5. γ-Endorphin(MW 1,859)
6. β-Endorphin (MW 3,465)

Peptides (MW 500-3,500)

2

3

1

5

4

120 Å

6

ColumnEluent: 150 X 4.6 mml.D.
: A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
20-40%B (0-20 min)Flow rate: 1.0 mL/min
Temperature : 37°C
Detection: UV at 220 nm
Injection: 10 μL (0.167 mg/mL)

TA12S05-01

2

1

3
5

4

120 Å

6
TAS12S11-01

0.0

5.0

2.5

7.5

10.0

12.5

15.0

17.5

min

Triart Prep C18-S has identical selectivity to analytical Triart C18. A method established with analytical Triart C18 can be directly transferred to preparative
scale with Triart Prep C18-S material.

Selection of optimal stationary phase

—
—
—
—
—
—

Insulin and small proteins (MW 4,300-17,000)

1

23 4
5 6

120 Å

S121214A

1

ColumnEluent: 150 X 4.6 mml.D.
: A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
25-60%B (0-20 min)Flow rate: 1.0 mL/min
Temperature : 37°C
Detection: UV at 220 nm
Injection: 10 μL (0.1-0.2 mg/mL)

4
6

2 3

5

200 Å
S121214B

0.0

2.0

4.0

6.0

1. Cytochrome c(MW 12,400)
2. Insulin (bovine) (MW 5,700)
3. Amyloid β-protein (MW 4,300)
4. Lysozyme(MW 14,300)
5. α-Lactalbumin(MW 14,100)
6. Myoglobin(MW 17,000)

8.0 10.0 12.0 14.0 16.0 18.0 min

Proteins with molecular weight (MW) of 10,000 or larger are effectively separated with Triart Prep C8-S while there is little difference in separation of proteins
with MW of less than 10,000 between Triart Prep C18-S and Triart Prep C8-S. It is useful to select optimal phase for establishing effective preparative method.

Excellent chemical durability
Alkaline condition (pH 11.5, 50˚C)% of initial retention time

Packing Materials

100
80
60

Conventional silica based C18

40

Columns are kept in acetonitrile/water/TFA(10/90/1, pH 1) at 70˚C, and tested for
performance every 20 hrs.

20
0
0

20

40

60

80

% of initial theoretical plate number

Acidic condition (pH 1, 70˚C)

120

120
100
80
60

20

100

0
0

Tim e (h r)Column: 10 μm, 120 Å, 250 X 4.6 mmI.D.
Eluent: acetonitrile/water (60/40)
Flow rate: 1.0 mL/min
Temperature : 37°C
Detection: UV at 254 nm
Sample:n-butyl benzoate

Conventional silica based C18

40

100

200

300

Tim e (h r)ColumnEluent: 10 μm, 120 Å, 150 X 4.6 mmI.D.
: 50 mM triethylamine in water/
50 mM triethylamine in methanol (80/20)
Flow rate: 1.0 mL/min
Temperature : 50°C
Detection: UV at 254 nm
Sample: caffeine

Triart Prep materials provide strong acidity-proof in the lower pH condition and alkaline-proof in the higher pH. These features enables purification with a mobile
phase containing TFA and cleaning with alkaline solution, which are often used on peptides and proteins purification.

130Regeneration with alkaline solution

Result

Test procedure
Sample injection(insulin in human serum)
Repeated 20 times

AU
2.0

Triart Prep C8-S
(10 μm, 200 Å)

AU
2.0

Initial

1.0

1.0

0.0

0.0
0.0

0.0

Cleaning with alkaline
solution
0.1 M NaOH/acetonitrile (50/50)
10 column volumes

1st injection

1.0

2.0

3.0

AU
2.0

AU
2.0

1.0

2.0

3.0

4.0 min

3.0

4.0 min

3.0

4.0 min

4.0 min

1.0

20th injection

Change inperformance

After 10cleaning cycles

0.0
0.0

1.0

2.0

1.0
AU
2.0
0.0
0.0

Neutralization and cleaning
with organic solvent
1. acetonitrile/water (20/80)
2. acetonitrile/water (90/10)

1.0

2.0

3.0

4.0 min

After 35
cleaning cycles

0.0
0.0
AU

AU
2.0

ColumnEluent

1.0

1.0

0.0
0.0

1.0

2.0

After regeneration

2.0: 50 X 4.6 mmI.D.
: A) water/TFA (100/0.1)
B) acetonitrile
29-36%B (0-2 min), 36%B (2-3 min),
29%B (3-6 min)Flow rate: 1.0 mL/min
Temperature : 25˚C
Detection: UV at 220 nm
Sample: 10 mg/mL bovine insulin/human serum (2/1)
Injection: 6 μL

1.0

1.0

2.0

3.0

4.0 min

Good peak shape
After 70
cleaning cycles

0.0
0.0

1.0

2.0

3.0

4.0 min

After repeated injection of crude serum sample, absorption of protein and/or other impurities on the surface of the packing material sometimes results in poor
peak shape or degradation of retention capacity. In such case, alkaline washing procedure is generally adopted for regeneration and removing impurities on thepacking materials. Hybrid silica based Triart Prep which shows strong resistance at high pH allows the effective cleaning of the gel with alkaline solution. Thisfeature provides highly cost-effective purification of target compounds.

Packing Materials
131

13

Packing Materials
Packing materials

YMC＊GEL HG
High density and high strength silica gel
Excellent mechanical suitability
Narrow distribution of particle size and pore size

Particle size: 10, 15, 20, 50 μm

High strength packing material
YMC＊GEL HG is packing material based on high density and high strength silica gel. Excellent mechanical stability allows them tobe used for a long term repacking into the dynamic axial compression column. YMC＊GEL HG packing materials have same chemical
modifications as YMC-Pack columns. This feature offers smooth and easy scale up from analytical to preparative conditions with highsample loading.

Excellent mechanical stability
SEM images

Pressure (MPa/m)

25
ODS-A-HG (15 μm, 120 Å)Competitor D (15 μm, 120 Å)

20

ODS-A-HG

Competitor D

n=1

n=1

n=13

n=13

15
10
5
0

5

1

10
Packing number

Conditions of column packing
Column size : 100 X 50 mmI.D., Packing pressure : 6.5 MPa
Conditions of pressure measurement
Eluent : methanol/water (85/15), Flow rate : 50 mL/min
High packing mechanical stability of YMC＊GEL HG is demonstrated by means of repeated of a dynamic axial compression column (DAC). Even after morethan 10 repacking cycles for the same material the pressure does not increase. The absence of fines is proven by a constant backpressure.

Easy scale up from analytical to preparative
Analysis
Particle size : 5 μm
Column: YMC-Pack ODS-AQ (120 Å)
250 X 4.6 mmI.D.
Flow rate: 1.0 mL/min
1

3

Packing Materials

2

Identical selectivity

1
3

2

P140210B

P140210A

0

5

10Eluent: acetonitrile/water (60/40)
Temperature : ambient
Detection: UV at 270 nm
Sample: 1. Uracil
2. Methyl benzoate
3. Naphthalene

132

Purification
Particle size : 50 μm
Column: ODS-AQ-HG (120 Å)
250 X 10 mmI.D.
Flow rate: 4.7 mL/min

min

0

5

10

min

YMC＊GEL HG packing materials have same chemical modifications as YMC-Pack columns. This feature offerssmooth and easy scale up from analytical to preparative conditions with high sample loading.

Packing materials

CHIRAL ART
Particl size: 3, 5, 10, 20 µm

Applicable to various chiral compounds
Excellent peak shape under high loadingExtremely low initial cost on analysis and purification

Packing materials with polysaccharide derivatives as chiral selector
CHIRAL ART are packing materials immobilized/coated with polysaccharide derivatives as chiral selector. CHIRAL ART immobilized
type can be used either in normal- or reversed-phase modes. CHRAL ART are suitable for separations of wide range of chiralcompounds, cis-trans isomers and geometric isomers. Packing materials are available in large quantities (multi kg).

Specifications
Immobilized type
Packing material

Particle size
(μm)

USP
Classification

Chiral selector

CHIRAL ART Amylose-SA

L99
Amylose tris(3,5-dimethylphenylcarbamate)
3,5-Dimethylphenylcarbamate–

CHIRAL ART Cellulose-SB
3
5
10
20

Cellulose tris(3,5-dimethylphenylcarbamate)

CHIRAL ART Cellulose-SC

L119
Cellulose tris(3,5-dichlorophenylcarbamate)

3,5-Dichlorophenylcarbamate–

CHIRAL ART Cellulose-SJ
Cellulose tris(4-methylbenzoate)
Usable mobile
phase

4-Methylbenzoate

Normalphasen-hexane, n-heptane, methanol, ethanol, 2-propanol, acetonitrile, ethyl acetate,tetrahydrofuran, chloroform, t-butyl methyl ether, etc.

Reversedphaseacetonitrile, methanol, ethanol, 2-propanol, tetrahydrofuran, water, aqueous buffer, etc.

Packing material

Particle size
(μm)

USP
Classification

Chiral selector

CHIRAL ART Amylose-C Neo

L51
3
5
10
20

Packing Materials

Coated type

Amylose tris(3,5-dimethylphenylcarbamate)
3,5-Dimethylphenylcarbamate

L40

CHIRAL ART Cellulose-C
Cellulose tris(3,5-dimethylphenylcarbamate)
Usable mobile phasen-hexane, n-heptane, ethanol, 2-propanol, acetonitrile, etc.

* Inquire us for the Amylose-C＊ See pp.62-66 for details of CHIRAL ART.

133

13

Packing Materials
Packing materials

BioPro IEX SmartSep Q/S
BioPro IEX Q/SUsable pH range : 2-12

 ‌High productivity on purification ‌Hydrophilic polymer beads with low nonspecific adsorption
High binding capacity and high resolution over a wide range of flow rate
Suitable for purification of antibodies, proteins and nucleic acids

Ion exchange media for purification of biopharmaceuticals
BioPro IEX media are available in strong ion exchangers of hydrophilic porous polymer beads with low nonspecific adsorption andhigh binding capacity.
BioPro IEX SmartSep Q/S are suitable for intermediate purification step and polishing step of biopharmaceuticals. BioPro IEXSmartSep Q/S show high resolution and recovery even at a high flow rate and high loading condition.
BioPro IEX Q/S are designed for capture and intermediate purification of proteins and nucleotides. High dynamic binding capacity
and high recovery allow fast purification process at large scale. It offers high productivity on industrial purification.

Specifications
BioPro IEX SmartSep Q

BioPro IEX SmartSep S

MatrixBioPro IEX Q

BioPro IEX S

Hydrophilic porous polymer

Particle size (μm)
Charged group10, 20, 30−R-N+(CH3)3

Ion exchange capacity(meq/mL-resin)
Dynamic binding capacity(mg/mL-resin)

75-R-N+(CH3)3-R-SO3–-R-SO3–> 0.08> 100 (BSA)> 0.10> 100 (lysozyme)

Usable pH range> 160 (BSA)> 160 (lysozyme)

2-12High dynamic binding capacity (DBC) for various samples
120

BioPro IEX SmartSep S30

Conditions of DBC measurement*
Column: 50 X 5.0 mmI.D.
Flow rate: 400 cm/hr (1.32 mL/min)
Temperature : 25°C

Brand T（porous S type 30 μm）

Brand G（porous S type 30 μm）

DBC (mg/mL-resin)

10080*Inquire us for details.

60

Particle
size
(μm)

40

Packing Materials

20
0Insulin(MW 5,800)

Lysozyme(MW 14,300)

InsulinLysozyme

Human
Polyclonal IgG

BioPro IEX SmartSep S30

30

73

111

93

Brand T (porous S type)

30

67

72

42

Brand G (porous S type)

30

64

85

41

BioPro IEX media have higher DBC compared to conventional ion exchange media. Especially for IgG, BioPro IEX media have more than twice as high DBC ascompetitors' media. This feature of BioPro IEX media make purification productivity of IgG per unit time double or more.

＊ See pp.27-29 for details of BioPro IEX media.

134

Human Polyclonal IgG(MW 150,000)

DBC (mg/mL-resin, 10% breakthrough)