Inverted Confocal
Raman Imaging

alpha300 Ri

3D Raman image of banana pulp

https://raman.oxinst.com

alpha300 Ri

Inverted Raman imaging for chemical
analysis of liquid or bulky specimens
The alpha300 Ri turns 3D chemical imaging upside down. Its inverted beam pathpreserves all of the functionality and high performance of WITec’s alpha300 confocal
Raman microscope series while introducing a new angle in access and handling.

Confocal Raman imaging is a powerful andnondestructive method for visualizing a sample’s
molecular composition in 2D and 3D. WITec’salpha300 confocal Raman imaging microscopes
provide high speed, sensitivity and resolution
simultaneously for fast and comprehensive sample
analysis.
The ability to view and investigate samples from below
is a great advantage when working with aqueous
solutions or oversized specimens. Studies in life
sciences, biomedicine, pharmaceutics and geosciencesin particular will benefit from the flexibility andperformance provided by the alpha300 Ri.

Key features•

Nondestructive, label-free chemical characterization•Convenient handling of liquid and bulky samples•Confocal beam path, which enables 3D image generation•Spatial resolution limited only by physical law•

Modular design and extensive upgrade options•Compatibility with other microscopy techniques including:fluorescence, dark field, bright field, differential
interference contrast (DIC) and phase contrast

aB

DAPI

Intensity (a.u)

Many accessories and upgrade possibilities developed
for the WITec alpha300 series are compatible with thealpha300 Ri. Other techniques associated with inverted
microscopes, such as fluorescence microscopy, can beeasily integrated, which enables correlative imaging.

Raman shift (cm-1)(A) Correlative Raman – fluorescence microscopy image of eukaryotic cells. Nuclei were stained with DAPI (blue). Endoplasmic reticulum (red) andnucleoli (green) were identified by their Raman signals. (B) Raman spectra corresponding to nucleoli (green) and endoplasmic reticulum (red).

An inverted beam path allows liquidsamples to be placed on the fixed planeof the stage for quick and repeatablemeasurements.

Bulky samples that would bechallenging to investigate
underneath a conventional
microscope objective turretcan be accommodated on
the stage of the alpha300 Ri.

The motorized sample stage facilitates
the mounting of environmental
enclosures and other accessories.

alpha300 S:
Scanning Near-field
Optical Microscopealpha300 A:
Atomic ForceMicroscopealpha300 R:
Confocal Raman
Microscopealpha300 apyron™: AutomatedConfocal Raman Microscope

We take care – WITec uses environmentally friendly printed materials.
While this policy is only a small contribution to a healthy environment, we at
WITec believe that focusing on details can effect positive change in the world.

WITec Microscopesalpha300 Ri:
Inverted Confocal
Raman Microscope

RISE®: Raman Imagingand Scanning Electron
Microscopealpha300 access:Confocal Micro-Raman System

Find your regional WITec and Oxford Instruments
contact at https://raman.oxinst.com/contact
WITec Headquarters
WITec Wissenschaftliche Instrumente und Technologie GmbH
Lise-Meitner-Str. 6, D-89081 Ulm, Germany
Phone +49 (0) 731 140 700, Fax +49 (0) 731 14070 200
info@witec.de, https://raman.oxinst.com

Explore the alpha300 Ri
product pagehttps://raman.oxinst.com/products/
raman-microscopes/alpha300ri