MSDS-CFE140417E

Japan Institute for the Control of Aging (JaICA)

MATERIAL SAFETY DATA SHEET
Date printed:

April 17, 2014

Date updated:
SECTION 1: PRODUCT AND DATA SHEET
Product Name:MC reagent Iron assay kit ( Ferrozine method )Product code:

CFE-005

Manufacture:

Japan Institute for the Control of Aging (JaICA),
Nikken SEIL Co., Ltd.

Address:

710-1 Haruoka, Fukuroi, Shizuoka 437-0125, Japan

Emergency Phone:+81-538-49-0125

Phone:+81-538-49-0125

FAX:+81-538-49-1267

SECTION 2: COMPOSITION/INFORMATION ON INGREDIENTS
CAS
NumberConcentr
ation

MWFormulaComponent
R-A:
Sodium Acetate

127-09-3< 1 wt%82,03

C2H3NaO2

Guanidine Hydrochloride

50-01-1< 30 wt%

95.53

HN=C(NH2)2HCl

Octylphenoxypolyethoxyetha
nol Nonionic Surfactant

9002-93-1< 1 wt%-(C2H4O)nC14H22O

L-Ascorbic Acid

50-81-7< 0.5 wt%

198.11C6H7NaO6

28048-33-1< 0.5wt%

514.4

C20H12N4Na2O6S2
xH2O

151-21-3< 0.5wt%

208.3

C12H25NaO4S

MES monohydrate

145224-94-8< 0.5wt%

213,25

C6H13NO4S · H2O

STD:
Nitric acid7697-37-2< 0.1 wt%

63.01

HNO3

R-R:
3-(2-Pyridyl)-5,6-bis(4-sulfop
henyl)-1,2,4-triazine
Disodium Salt Hydrate
Sodium Dodecyl Sulfate

1 / 10SECTION 3: HAZARDS IDENTIFICATION
Component
Sodium Acetate

Guanidine
Hydrochloride

Identification and Emergency Overview
Classification of the substanceNot a hazardous substance or mixture according to Regulation (EC) No. 1272/2008.
Label elements
The product does not need to be labelled in accordance with EC directives orrespective national laws.
Other hazards: none
Classification of the substanceAcute toxicity, Oral (Category 4)
Skin irritation (Category 2)
Eye irritation (Category 2)
Classification according to EU Directives 67/548/EEC oR-A999/45/EC
Harmful if swallowed. Irritating to eyes and skin.
WARNING

Octylphenoxypolyethoxyethanol NonionicSurfactant

Hazard statement(s):H302 Harmful if swallowed / H315 Causes skin irritation.
H319 Causes serious eye irritation.
Precautionary statement(s)P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental Hazard
Statements: none
R-phrase(s): R22 Harmful if swallowed. / R36/38 Irritating to eyes and skin.
S-phrase(s): S22 Do not breathe dust.
Other hazards: None
EMERGENCY OVERVIEW: Harmful by inhalation, in contact with skin and if
swallowed.
Category of Danger:
Harmful , Lachrymator
Principle routes of exposure: Skin
Inhalation: Harmful by inhalation.
Ingestion: Harmful if swallowed.
Skin contact: Harmful in contact with skin.
Eye contact: Risk of serious damage to eyes
Vapors extremely irritating to eyes an respiratory tract
ANSI Classification Irritant - eye, skin, respiratory, Irritant - eye, severe
Statements of hazard HARMFUL IF SWALLOWED. MAY BE HARMFUL IF
ABSORBED THROUGH SKIN OR INHALED.
CAUSES EYE IRRITATION.
Statement of Spill or Leak - ANSI Label Contain and/or absorb spill with inert
material (e.g. sand, vermiculite), then place in suitable container. Do not flush to
sewer or allow to enter waterways. Use appropriate Personal Protective
Equipment.
Statement of First Aid If swallowed, do NOT induce vomiting unless directed to do soby medical personnel. Never give anything by mouth to an unconscious person. Call aphysician. In case of contact, immediately flush eyes with plenty of water for at least
15 minutes. Call a physician. In case of contact, flush eyes with running water for atleast 15 minutes.
Consult a physician for irritation or any other symptom.
2 / 10L-Ascorbic Acid3-(2-Pyridyl)-5,6-bis(
4-sulfophenyl)-1,2,4-t
riazine Disodium Salt
HydratePrecautions - ANSI Label Do not taste or swallow. Wash thoroughly after handling.
Do not get in eyes, on skin, or on clothing. Wash thoroughly after handling. Avoid
breathing vapors. Avoid prolonged or repeated contact with the skin, eyes or
respiratory tract. Wash thoroughly after exposure.
Classification of the substanceNot a hazardous substance or mixture according to Regulation (EC) No. 1272/2008.
Label elements
The product does not need to be labelled in accordance with EC directives orrespective national laws.
Other hazards - none
HAZARDS IDENTIFICATIONPHYSICAL HAZARDS Not classified
HEALTH HAZARDSSkin corrosion/irritation Category 2
Serious eye damage/eye irritation Category 2A
ENVIRONMENTAL HAZARDS Not classified
WARNING

Sodium Dodecyl
SulfateHazard statement: Causes skin irritation. Causes serious eye irritation
Precautionary statements[Prevention] Wash hands thoroughly after handling.
Wear protective gloves/eye protection/face protection.
[Response] IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses,if present and easy to do. Continue rinsing.If eye irritation persists:
Get medical advice/attention.
IF ON SKIN: Gently wash with plenty of soap and water.If skin irritation occurs: Get
medical advice/attention. Take off contaminated clothing and wash before reuse.
Classification of the substance
Flammable solids (Category 1)
Acute toxicity, Oral (Category 4)
Acute toxicity, Dermal (Category 3)
Skin irritation (Category 2)
Serious eye damage (Category 1)
Specific target organ toxicity - single exposure (Category 3)
Classification according to EU Directives 67/548/EEC or 1999/45/EC
Highly flammable. Harmful in contact with skin and if swallowed. Irritating toeyes, respiratory system and skin.
Danger

MES monohydrate

Hazard statement(s): H228 Flammable solid. / H302 Harmful if swallowed.
H311 Toxic in contact with skin. / H315 Causes skin irritation.
H318 Causes serious eye damage. / H335 May cause respiratory irritation.
P210 Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P261 Avoid breathing dust.
P280 Wear protective gloves/ eye protection/ face protection.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue rinsing.
P312 Call a POISON CENTER or doctor/ physician if you feel unwell.
Supplemental Hazard Statements: none
Other hazards - none
Classification of the substanceSkin irritation (Category 2)
Eye irritation (Category 2)
Specific target organ toxicity - single exposure (Category 3)
Classification according to EU Directives 67/548/EEC oR-A999/45/EC
Irritating to eyes, respiratory system and skin.
3 / 10Warning

Nitric acidHazard statement(s):H315 Causes skin irritation/H319 Causes serious eye irritation.
H335 May cause respiratory irritation.
Precautionary statement(s)P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental Hazard Statements none
R-phrase(s): R36/37/38 Irritating to eyes, respiratory system and skin.
S-phrase(s): S26 In case of contact with eyes, rinse immediately with plenty of waterand seek medical advice. / S36 Wear suitable protective clothing.
Other hazards - none
GHS CLASSIFICATION :Skin corrosion/irritation: Category 2
Eye damage/Eye irritation: Category 2
WARNINGHAZARD STATEMENTS :H315 Causes skin irritation
H319 Causes serious eye irritation
PRECAUTIONARY STATEMENTS :P280 Wear protective gloves/protective
clothing/eye protection/face protection.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue rinsing.
EU CLASSIFICATION: according to Directive 67/548/EEC
SYMBOL : Xi
R-phrase : R36/38 Irritating to eyes and skin.
S-phrase : S26 In case of contact with eyes, rinse immediately with plenty of waterand seek medical advice.
S36/37/39 Wear suitable protective clothing, gloves and eye/face protection.

SECTION 4: FIRST AND MEASURE
Description of first aid measures· General information
No special measures required.
· After inhalation Supply fresh air;Consult doctor in case of complaints.
· After skin contact
Generally the product does not irritate the skin.
· After eye contact
Rinse opened eye for several minutes under running water.
· After swallowing
If symptoms persist consult doctor.
· Information for doctor· Most important symptoms and effects, both acute and delayed No further relevant information available.
· Indication of any immediate
Medical attention and special treatment needed
No further relevant information available.

SECTION 5: FIRE-FIGHTING MEASURES· Extinguishing media· Suitable extinguishing agents: CO2, powder or water spray. Fight larger fires with water spray or alcohol
4 / 10resistant foam.
· Special hazards arising from the substance or mixture· Advice for firefighters· Protective equipment:

No further relevant information available.
No special measures required.

SECTION 6: ACCIDENTIAL RELEASE MEASURES· Personal precautions, protective equipment and emergency procedures Not required.
· Environmental precautions:
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
· Methods and material for containment and cleaning up: Absorb with liquid-binding material.
· Reference to other sections
See Section 7 for information on safe handling
See Section 8 for information on personal protection equipment.
See Section 13 for disposal information.

SECTION 7: HANDLING AND STORAGE
Handling· Precautions for safe handling: Keep container tightly sealed.
· Information about fire - and explosion protection: No special measures required.
Conditions for safe storage, including any incompatibilities· Storage: Store in at 4 °C.
· Requirements to be met by storerooms and receptacles: No special requirements.
· Information about storage in one common storage facility: Not required.
· Further information about storage conditions: KOH is air sensitive, strongly hygroscopic.
· Specific end use(s) No further relevant information available.

SECTION 8: EXPOSURE CONTROL/PERSONAL PROTECTION
Hygienic measures
Follow the usual precautionary measures for handling chemicals. Wear suitable protective equipment andclothing.
Keep away from food and beverages.
Remove all soiled and contaminated clothing immediately.
Avoid contact with skin and eyes.
Wash hands thoroughly after handling.
Personal protective equipment
Use suitable respirator when high concentration are present. Wear lab coat, gloves and splash goggles.

SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES· Information on basic physical and chemical properties· General Information· Appearance:Form:liquidColour:Different according to colouring· Odour:Odourless· Odour threshold:Not determined.
· pH-value: Not determined.
· Change in condition Melting point/Melting range: undetermined
Boiling point/Boiling range:
undetermined· Flash point:
Not applicable· Flammability (solid, gaseous)
Not applicable.
· Ignition temperature:Decomposition temperature: Not determined.
· Self-igniting:
Product is not selfigniting.
5 / 10· Danger of explosion:· Explosion limits: Lower:
Upper:· Vapour pressure:· Density:· Relative density· Vapour density· Evaporation rate
· Solubility in / Miscibility with Water:· Partition coefficient (n-octanol/water):· Viscosity:
dynamic:
kinematic:· Solvent content: Organic solvents:
Solids content:· Other information

Product does not present an explosion hazard.
Not determined.
Not determined.
Not determined.
Not determined
Not determined.
Not determined.
Not determined.
Fully miscible
Not determined.
Not determined.
Not determined.
0,0 %
1,0 %
No further relevant information available.

SECTION 10: STABILITY AND REACTIVITY· Reactivity· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
· Possibility of hazardous reactions
No dangerous reactions known· Conditions to avoid
No further relevant information available.
· Incompatible materials:
No further relevant information available.
· Hazardous decomposition products:
No dangerous decomposition products known

SECTION 11: TOXICOLOGICAL INFORMATION
Sodium Acetate
Information on toxicological effects
Acute toxicity
LD50 Oral - rat 3.530 mg/kg
LC50 Inhalation - rat - 1 h > 30.000 mg/m3
LD50 Dermal - rabbit > 10.000 mg/kg
Skin corrosion/irritation
Skin - rabbit Mild skin irritation - 24 h
Serious eye damage/eye irritation
Eyes - rabbit Mild eye irritation
Respiratory or skin sensitization
No data available
Germ cell mutagenicity
No data available
Carcinogenicity
IARC: No component of this product present at levels greater than or equal to
0.1% is identified asprobable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
No data available
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
No data available
Aspiration hazard
No data available
Potential health effects
Inhalation: May be harmful if inhaled. May cause respiratory tract irritation.
Ingestion: May be harmful if swallowed.
Skin: May be harmful if absorbed through skin. May cause skin irritation.
Eyes: Causes eye irritation.
Signs and Symptoms of Exposure
Abdominal pain, Nausea, Vomiting
Additional Information
RTECS: AJ4300010
Guanidine Hydrochloride
6 / 10Information on toxicological effects
Acute toxicity
No data available
Skin corrosion/irritation
No data available
Serious eye damage/eye irritation
No data available
Respiratory or skin sensitization
No data available
Germ cell mutagenicity
No data available
Carcinogenicity
IARC:
3 - Group 3: Not classifiable as to its carcinogenicity to humans(1-Ethenyl-2-pyrrolidinone homopolymer)
IARC:
3 - Group 3: Not classifiable as to its carcinogenicity to humans(Kieselguhr)
Reproductive toxicity
No data available
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
No data available
Aspiration hazard
No data available
Potential health effects
Inhalation: May be harmful if inhaled. Causes respiratory tract irritation.
Ingestion: Harmful if swallowed.
Skin: May be harmful if absorbed through skin. Causes skin irritation.
Eyes: Causes serious eye irritation.
Signs and Symptoms of Exposure
To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly
investigated.
Additional Information
RTECS: Not available
Octylphenoxypolyethoxyethanol Nonionic Surfactant
Acute toxicity
Oral LD50 Rat : 1800 mg/kg
Chronic toxicity: Chronic exposure may cause nausea and vomiting, higher exposure causes unconsciousness.
Local effects: Symptoms of overexposure may be headache, dizziness, tiredness, nausea and vomiting.
Specific effects: May include moderate to severe erythema (redness) and moderate edema (raised skin), nausea,
vomiting, headache.
Primary irritation:
No data is available
Carcinogenic effects:
No data is available
Mutagenic effects:
No data is available
Reproductive toxicity:
No data is available
L-Ascorbic Acid
Information on toxicological effects
Acute toxicity
LD50 Oral - rat 11.900 mg/kg
Remarks: Sense Organs and Special Senses (Nose, Eye, Ear, and Taste):Eye:Lacrimation.
Behavioral:Somnolence (general depressed activity). Diarrhoea
Skin corrosion/irritation
No data available
Serious eye damage/eye irritation
No data available
Respiratory or skin sensitization
No data available
Germ cell mutagenicity
Genotoxicity in vitro - mouse - Liver
Genotoxicity in vivo - mouse - Intraperitoneal
Micronucleus test IARC:
No component of this product present at levels greater than or equal to
0.1% is identified as probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
No data available
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure No data available
Aspiration hazard
No data available
Potential health effects
Inhalation
May be harmful if inhaled. May cause respiratory tract irritation.
Ingestion
May be harmful if swallowed.
Skin
May be harmful if absorbed through skin. May cause skin irritation.
Eyes
May cause eye irritation.
Signs and Symptoms of Exposure
Chronic ingestion of large doses may cause gastrointestinal disturbances including nausea and diarrhea,
7 / 10urinary effects involving urine acidification, oxalate and uric crystallizaton in the bladder and kidney, anddecreased reaction times and psychomotor coordination.
Additional Information
RTECS: CI7650000
3-(2-Pyridyl)-5,6-bis(4-sulfophenyl)-1,2,4-triazine Disodium Salt Hydrate
Acute Toxicity:
No data available
Skin corrosion/irritation:
No data available
Serious eye
damage/irritation:
No data available
Germ cell mutagenicity: No data available
Carcinogenicity:
IARC
No data available
NTP
No data available
Reproductive toxicity:
No data available
RTECS Number: DB7345000
Sodium Dodecyl Sulfate
Acute Toxicity:

ihl-ratLC50:
ipr-ratLD50:
orl-ratLD50:
skn-rbt
LDLo:
Skin corrosion/irritation:
skn-rbt
skn-hmn
Serious eyedamage/irritation: eye-rbt
Germ cell mutagenicity:
dni-hmn-lym
mmo-omimmo-smc
Carcinogenicity:
IARC
NTP
Reproductive toxicity:
RTECS Number: WT1050000>3900 mg/m3/1H
210 mg/kg
1288 mg/kg
10 g/kg
250 mg/24H MOD
0.1 %/24H MOD
100 mg/24H MOD
100 mg/L
200 mg/L (-S9)
3500 umol/L (-S9)
No data available
No data available
No data available

MES monohydrate
Information on toxicological effects
Acute toxicity
Skin corrosion/irritation
Serious eye damage/eye irritation
Respiratory or skin sensitization
Germ cell mutagenicity
Carcinogenicity

No data available
No data available
No data available
No data available
No data available
IARC:
No component of this product present at levels
greater than or equal to 0.1% is identified asprobable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
No data available
Specific target organ toxicity - single exposure
Inhalation - May cause respiratory irritation.
Specific target organ toxicity - repeated exposure No data available
Aspiration hazard
No data available
Potential health effects
Inhalation May be harmful if inhaled. Causes respiratory tract irritation.
Ingestion May be harmful if swallowed.
Skin
May be harmful if absorbed through skin. Causes skin irritation.
Eyes
Causes serious eye irritation.
Signs and Symptoms of Exposure
To the best of our knowledge, the chemical, physical, and toxicological properties have not beenthoroughly investigated.
Additional Information
RTECS:
Not available
Nitric acid
Acute toxicity (oral/dermal/inhalation) :LDLo(orl,human):
LC50(ihl,rat):
8 / 10430 mg/kg (YAKUD5 22,651,1980)
130mg/m3/4H(VCVN5* -,45,1993)

TDLo(skin,rat):
150mL/kg(VCVN5* -,45,1993)
Harmful if inhaled and ingested.
Causes skin irritation.
Causes eye irritation.
No data available
No data available
TDLo(orl,rat): 21150mg/kg(1-21 D preg)(ZHYGAM 29,667,1983)
TDLo(orl,rat): 2345mg/kg(18 D preg)(ZHYGAM 29,667,1983)
Specific target organ toxicity - single exposure:
No data available
Specific target organ toxicity - repeated exposure: No data available
Aspiration hazard:
No data available
Carcinogenicity:
No data available
Additional Information:
NTP :Not listed
IARC :
Not listed
OSHA : Not listed
ACGIH : Not listed
Toxicity data :Skin Corrosion/ irritation:
Eye Damage/Eye irritation:
Respiratory Or Skin sensitization:
Germ cell mutagenicity:
Reproductive toxicity:

SECTION 12: ECOLOGICAL INFORMATION
Sodium Acetate
Toxicity Toxicity to fish
Toxicity to daphnia andother aquatic invertebrates
Persistence and degradability

LC50 - Pimephales promelas (fathead minnow) - 13.330 mg/l - 120 h
LC50 - Lepomis macrochirus (Bluegill) - 5.000 mg/l - 24 h
EC50 - Daphnia magna (Water flea) - > 1.000 mg/l - 48 h
Biodegradability Result

99 % - Readily biodegradable.

Octylphenoxypolyethoxyethanol Nonionic Surfactant
Aquatic toxicity: May cause long-term adverse effects in the aquatic environment.
Sodium Dodecyl Sulfate
Bioaccumulative potential(BCF):
Mobillity in soil
log Pow:
Soil adsorption (Koc):
Henry's Law constant(PaM3/mol):
Ecotoxicity effects: No data available

71
1.60
1.0 x 104
1.9 x 10-2

SECTION 13: DISPOSAL CONSIDERATIONS
Waste treatment methods· Recommendation Dispose of waste according to applicable local, state, and federal regulations.
Uncleaned packaging:· Recommendation: Dispose of packaging according to applicable local, state, and federal regulations.
· Recommended cleansing agents: Water, if necessary together with cleansing agents.

SECTION 14: TRANSPORT INFORMATION· UN-Number
ADR, IMDG, IATA
· UN proper shipping name ADR, IMDG, IATA
· Transport hazard class(es) ADR, IMDG, IATA
· Packing group
ADR, IMDG, IATA
· Environmental hazards:
Marine pollutant:· Special precautions for user
· Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code9 / 10Void
Void
Class Void
Void
No
Not applicable.
Not applicable

SECTION 15: REGULATORY INFORMATION· Safety, health and environmental regulations/legislation specific for the substance or mixture· GHS label elements
Void· Hazard pictograms
Void· Signal word
Void
· Hazard statements
Void· National regulations· Waterhazard class:
Water hazard class 2 (Self-assessment): hazardous for water.
· Chemical safety assessment:
A Chemical Safety Assessment has not been carried out.

SECTION 16: OTHER INFORMATION INCLUDING INFORMATION ON PREPARATION
AND REVISION OF THE MSDS
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
· Relevant phrases
H271
May cause fire or explosion; strong oxidiser.
H300
Fatal if swallowed.
H302
Harmful if swallowed.
H310
Fatal in contact with skin.
H314
Causes severe skin burns and eye damage.
H330
Fatal if inhaled.
H332
Harmful if inhaled.
R19
May form explosive peroxides.
R20/22Harmful by inhalation and if swallowed.
R23/24/25 Toxic by inhalation, in contact with skin and if swallowed.
R34
Causes burns.
R8
Contact with combustible material may cause fire.
The above information is believed to be correct to be the best of our knowledge and information but does not
purport to be all inclusive and shall be used only as a guide. This product is intended to be used by expert
persons having chemical knowledge and skill, at their own discretion and risk and the manufacturer shall not
be held liable for any damage resulting from handling or from contact with the above material.

10 / 10