Division of Biofunctional Chemistry
The Chemical Society of Japan
Vol. 22, No.1 (2007. 5. 31)

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l¼ BL276.H'** ±±,*Ps ARS &-GA-7K tRNA «©
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-GA-7Kc_* s ARS ª¬*y-GA°³(+ )r
y-GA°,_*  " p ARS , -GA-7Kc £ gy-GA°x-GA-7Kc,§  * p ARS 93JO?N4 }-4-+|¡µ/E>Ýd´,& Ç}àkh., ±nÆ/E>Ýeá'|N»Å* -*uã.`§ ,`Þ 46 zyS RNA ~,?K365
0F.ß·çw 3è12)?K3650F 3, 5-7=;LAM7Jyªl/E>Ý.ytRNA  3'/E>Ý.Âp,  ,â?K3650F tRNA
 3'x,a×ÜcçACCèOÖ/E>Ýªys $.ÍÏ,&VtRNA Vµ/E>Ý.Âp, nÆ,] !- N-G9J/E>Ý(136ÝçY ¥Ýy/E>ÝäXZèD Z/E>Ý)µ/E>Ý'y d´ ,&l¸/E>/6Jl©q¡d´ ,µ/E>ÝeÀ #
?K3650F68:F 2JC36Jy. 3, 5-7=;LAM7Jyªlµ/E>ÝtRNA?K3650F.N«p,µ/E>/6J tRNA .ÎË,  #¿Ë'¨£[RÒ+,  68:F l¸/E>/6Jl©¤Ó,&¾^l-ÉÌ ,&68:F ÚWoI@L4HEM4Þ,|ºäµ/E>Ý.r%DIB@9<Ãp¦´.P ,hyÉ+,
êé Tß 
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Integrated Discovery system : RaPID 68:Ft"è ½ nÆ,±x ´¼væÈfÁ68:F.å\DIB@9<¼¶.ÐÑB@9<gÈ¹
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mÄ°
1) C. J. Noren, S. J. Anthony-Cahill, M. C. Griffith, P. G. schultz, Science, 244, 182 (1989)
2) J.D. Bain, C.G. Grave, T.A.. Dix, A.R. Chamberlin, E.S. Diala, J. Am. Chem. Soc., 111, 8013
(1989)
3) T. Hohsaka, K. Sato, M. Sisido, K. Takai, S. Yokoyama, FEBS Lett., 344, 171 (1994)
4) T. Hohsaka, Y. Ashizuka, H. Murakami, M. Sisido, J. Am. Chem. Soc., 118, 9778 (1996)
5) I. Hirao, M. Kimoto, T. Mitsui, T. Fujiwara, R. Kawai, A. Sato, Y. Harada, S. Yokoyama, Nat.
Methods, 3, 729 (2006)
6) J. D. Bain, C. Switzer, A.R. Chamberlin, S. A. Benner, Nature, 356, 537 (1992)
7) A. C. Forster, Z. Tan, M. N. L. Nalam, H. Lin, H. Qu, V. W. Cornish, S. C. Blacklow, Proc. Natl.
Acad. Sci. USA , 100, 6353 (2003)
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ERTeBN
1.

Grynkiewicz, G., Poenie, M. & Tsien, R. Y. A new generation of Ca2+ indicators with greatly improved
fluorescence properties. J. Biol. Chem. 260, 3440-3450 (1985).

2.

Tsien, R. Y. Fluorescence imaging creates a window on the cell. Chem. Eng. News 18, 34-44 (1994).

3.

Umezawa, Y. Genetically encoded optical probes for molecular processes in living cells. Trac-Trends
Anal. Chem. 24, 138-146 (2005).

4.

Sato, M. Imaging molecular events in single living cells. Anal. Bioanal. Chem. 386, 435-443 (2006).

5.

Sato, M., Ueda, Y., Takagi, T. & Umezawa, Y. Production of PtdInsP3 at endomembranes is triggeredby receptor endocytosis. Nature Cell Biol. 5, 1016-1022 (2003).

6.

Miyawaki, A. & Tsien, R. Y. Monitoring protein conformations and interactions by fluorescenceresonance energy transfer between mutants of green fluorescent protein. Method. Enzymol. 327,472-500 (2000).

7.

Sato, M., Ueda, Y. & Umezawa, Y. Imaging diacylglycerol dynamics at organelle membranes. Nature
Methods 3, 797-799 (2006).

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1)

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Yagi, S.; Yonekura I.; Awakura, M.; Ezoe, M.; Takagishi, T. Chem. Commun. 2001, 557-558.

3)Ezoe, M.; Minami, T.; Ogawa, Y.; Yagi, S.; Nakazumi, H.; Matsuyama, T.; Wada K.; Horinaka H.
Photochem. Photobiol. Sci., 2005, 4, 641-646.

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Yagi, S.; Ezoe, M.; Yonekura, I.; Takagishi T.; Nakazumi H. J. Am. Chem. Soc. 2003, 125, 4068-4069.

5)Ezoe, M.; Yagi, S.; Nakazumi, H.; Itou, M.; Araki Y.; Ito O. Tetrahedron, 2006, 62, 2501-2510.

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