LC column catalog

L-column3

L-column3, High performance column for LC, Since 2017.

LC column catalog
Reversed phase column

L-column3

L-column, L-column2▶ Feature▶ Feature▶ UHPLC column▶ Line up▶ Metal-free column▶ Application data

L-column3
Ver. 02TM▶ Technical report▶ Line up

Realizing both excellent chemical stability
and top-level low adsorptivity
Unparalleled high-performance, all-around column

3
Bonded phase
C18, C8

PCS silica(Perfect chemical stable silica)

Perfect stable end-capping■ Improving chemical stability, so that usable within the range of pH 1 to pH 12.
■ Low adsorptivity is at the top level for various analytes.
■ Improvement of separation or peak shape, since the pH of an eluent is flexibly
selectable■ A 100% aqueous eluent can be stably analyzed.

1

Silica-based columns with the many advantages of high separation efficiency and mechanical strength are currently usedmost generally for the columns for reversed phase chromatography. On the other hand, the pH range of usable eluent islimited because exposure to the alkaline solution are easily dissolved silica.
L-column3 is an all-around column where the chemical stability (acid and alkaline resistance) of the packing materials is
dramatically improved by using newly developed and highly alkaline resistant PCS silica (perfect chemical stable silica)
and perfectly stable end-capping with performance much higher than that of L-column2.

L-column3 proposes the possibility of a new analysis.

L-column3 C18
Base materials

PCS silica (Perfect chemical stable silica)

Pore size

120 Å (12 nm)
340 m2/g

Specific surface area
Particle size

2 μm, 3 μm, 5 μm

Bonded phase

Octadecyl silyl (C18, ODS)

End-capping
USP category
Usable pH range

L-column3 C8

3 μm, 5 μm
Octyl silyl (C8, Octyl)

Perfect stable end-capping
L1

L7
pH 1 - pH 122

L-column3

L-column3 is chemically stable, so that usable within the range of pH 1 to pH 12.
L-column3 offers very high resistance to alkaline, and packing materials are designed to be robust. In addition, it performs similar highresistance to neutrality and acidity.
L-column3 can be used as an all-around column capable of method design for a wide range of pH values.
1

2

3

4

5

6

7

8

9

10

11

12

L-column2 pH 2 - pH 9
L-column3 pH 1 - pH 12

140
Stability of Retention data (%)

Stability of Retention data (%)

140k (Amitriptyline) / k (Acenaphthene)

130

120

L-column2

110

L-column3

100
0

100

200
Time (Hour)

300

k (Amitriptyline) / k (Acenaphthene)

L-column2

120

110

L-column3

100

400

0

■ Durability test (pH 1)

50

100
150
Time (Hour)

200

250

- k : Retention factor

Stability of N (Naphthalene) (%)

120[Durability test conditions]
Column: C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: CH3OH/1% TFA in H2O (10/90)
Flow rate: 0.2 mL/min; Temp.: 40°C

Amitriptyline

130

L-column3
100

80Brand A
Brand B
Brand C
Brand E
Brand F

60

40
0Acenaphthene

L-column2

50

100
150
Time (Hour)

200

250

■ Durability test (pH 12, triethylamine solution)
H3C

N
CH3

[Durability test conditions]
Column: C18, 5 μm; Size: 2.0 mm or 2.1 mm I.D., 150 mm L.
Eluent: CH3OH/54 mM TEA in H2O (10/90)
Flow rate: 0.2 mL/min; Temp.: 50°C
- k : Retention factor, N : Number of theoretical platesComparison columns are as follows:- Columns of other company: Usable up to pH 12.
- L-column2: Usable from pH 2 to pH 9.

120
Stability of N (Naphthalene) (%)

An alkaline eluent promotes the degradation of silica-based
columns. The dissolution of silica adds vacant space to thecolumn bed and decreases the number of theoretical plates.
The loss of the bonded phase and exposure of the silanolgroup over the surface of silica cause adsorption of basic
compounds into the silanol group, which results in a delay ofretention (see the technical report on P. 11). A strong acideluent is also subject to similar degradation, although thechange is slow.
L-column3 shows small changes and is stable in durability
tests at pH 1 and pH 12 (triethylamine solution andphosphate buffer solution).

L-column3
100

80

60

L-column2
40
0

50

200

250

■ Durability test (pH 12, phosphate buﬀer solution)[Durability test conditions]
Column: C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: CH3OH/10 mM Phosphate buffer (10/90)
Flow rate: 0.2 mL/min; Temp.: 40°C
- N : Number of theoretical plates

3

100
150
Time (Hour)L-column3

Low adsorptivity is at the top level in various analytes.
L-column3 shows a sharp peak not only for basic compounds but also for acidic compounds and coordination compounds.
L-column3 demonstrates low adsorptivity at the top level for various analytes and provides an ideal peak shape.
■ Basic compounds
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (35/65)
1. Paroxetine

2. Citalopram

F

NH
H
O

O

■ Coordination compound

Eluent: CH3CN/20 mM H3PO4 in H2O (2/98)

Eluent: CH3CN/20 mM H3PO4 in H2O (60/40)

1. Formic acid

3. Fluoxetine
H3C
NH

N
O

H

■ Acidic compounds

O

CF3

H

N CH3
H3C

O

2. Acetic acid

OH

H3C

2. Hinokitiol

1. Uracil
O

O

O

OH

OH

NH
N
H

O
CH3
H3C

F

L-column3

S(3) : 1.30

S(1) : 1.20

1S(2) : 1.08

3

1

L-column2

2

1

2

0

3. Benzene

O

3

2

5

10
Time (min)S(3) : 1.29

2

15

20

0

1

2
Time (min)

3

4

0

S(1) : 1.17

3

4

8Time (min)

16

12

16

12

16

12

16

S(2) : 1.06
1

1

12

2

3

1
2

0

Brand A

5

10
Time (min)

15

20

0

S(3) : 3.76 2

1

2
Time (min)

3

4

0

S(1) : 4.17

48Time (min)S(2) : 1.21
2

2

1

3

3

1
1
0

Brand C

5

10Time (min)

15

20

0

S(3) : 1.69

1

S(1) : 1.24

2Time (min)

3

4

0

4

S(2) : 3.83

1

2

Brand D

5

3

2
1

3

1

0

8
Time (min)210Time (min)S(3) : 1.21

15

20

0

1

2
Time (min)

3

1

2

4

0

S(1) : 1.13

2
3

1

4

8
Time (min)S(2) : 1.29
1
3

2

0

Brand E

5

10Time (min)S(3) : 1.60

15

20

0

1

2
Time (min)

3

4

0

1

2

3

1

8
Time (min)12

16

S(2) : 1.05

S(1) : 1.15

2

4

1
3
2

0

5

10
Time (min)

15

20

0

1

2
Time (min)

3

4

0

4

8
Time (min)1216[Analytical conditions] Column: C18, 5 μm; Size: 4.6 mm I.D., 150 mm L.; Flow rate: 1 mL/min; Temp.: 40°C; Inj. vol.: 1 μL
- S : Symmetry factor

4L-column3

Improvement of separation and peak shape, since the pH of an eluent is flexibly
selectable.
Widening of the pH range of a usable eluent remarkably enlarges the range of the method of analysis. The use of an alkaline eluent,especially, enables analyses where the dissociation of basic compounds is restricted, which is very advantageous.

16

3

7. Triprolidine
6. Fluvoxamine

2

Retention time (min)

1
5

pH 11

7
4
1
25

6

3

12

(Basiccompounds)

5. Fexofenadine(Amphoteric compounds)
4. Isopropyl 4-hydroxybenzoate(Weak acidity compounds)
3. Indometacin
2. Ibuprofen(Acidic
compounds)
1. ketoprofen

8

4

7

pH 7

6

4
0
2

3
7

5

pH 2

1

6

0

4

4

6
(pH)

8

10

12

2
- The polygonal line graph is showed the retention behavior. Actually, retention timenever linearly changes because of the existence of the buffer capacity or the
influence of the buffer salt.

48Time (min)1216

The change in the pH of an eluent changes the retention
behavior of ionic compounds. Generally, for acidic
compounds, higher pH results in less retention. Conversely,
in basic compounds, higher pH results in greater retention.
There is a strong possibility that the use of an alkaline eluent
provides a separation pattern different from the acidity andneutral ranges, thus an improvement in separation can be
expected.

■ Comparison of retention time (Drugs)[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: 25 mM Phosphate buffer
A/B, 20/80-70/30 (0-20 min)Flow rate: 0.3 mL/min; Temp.: 40°C; Detection: UV 220 nm
Inj. vol.: 1 μL

Amitriptyline

Sufficient retention
Increase of peak intensity

pH

H3C

High

(pKa 9.4)
NCH3

5 mM NH3 in H2O
5 mM HCO3NH4 in H2O
5 mM CH3COONH4 in H2O
5 mM HCOONH4 in H2O

Low
0

0.1% HCOOH in H2O
4

8

12

Time (min)■ Comparison of retention time (Basic drugs)[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: Aqueous solution
A/B, 40/60-90/10-90/10 (0-10-12 min)Flow rate: 0.3 mL/min; Temp.: 40°C; Detection: ESI-MS/MS (+)
Inj. vol.: 1 μL

Peak intensity is important in the LC/MS often used in trace
analysis. The high pH of an eluent in the analysis of basic
compounds provides sufficient retention and peak intensity.
This is the advantage of the use of an alkaline eluent.

5

L-column3

The preparation efficiency of basic compounds is improved by using an alkaline
eluent.
The use of an alkaline eluent remarkably improves the peak shape of basic compounds. Even a large injection volume does not cause
deviations in the retention time but provides a sharp peak. Consequently, the increase in the loads becomes possible, and preparation
efficiency is improved.
Homochlorcyclizine

N

CH3

N

pH 11

Number ofpeaks: 51

pH 7

pH 7

Number ofpeaks: 44

pH 2

pH 2

Number ofpeaks: 45

H

pH 11

0

Cl

5

10

15
20
Time (min)

25

30

35

0

■ Comparison of injection volume (Basic drugs)

5

25

30

35

[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: 25 mM Phosphate buffer
A/B, 5/95-75/25-75/25 (0-30-40 min)Flow rate: 0.2 mL/min; Temp.: 40°C; Detection: UV 220 nm
Inj. vol.: 5 μL; Sample: Homochlorcyclizine- The 10 g/L homochlorcyclizine added the hydrogen peroxide and left for 70 hours is
used.

- The 10 g/L homochlorcyclizine added the hydrogen peroxide and left for 70 hours isused.

Alkaline eluent

Alkaline eluent

10 mM HCOOH+NH3 in H2O (pH 11)
Inj. vol.: 20 μL (20 g/L)
Duloxetine

Scale-up

Load
400 μg5

10 mM HCOOH+NH3 in H2O (pH 11)
Column Size: 10.0 mm I.D., 150 mm L.
Flow rate: 4 mL/min
Inj. vol.: 100 μL (100 g/L)

Inner diameter of column
Flow rate
Injection volume
Sample concentration
10

15

0

5

10

Neutral eluent

Acidic eluent
10 mM HCOOH in H2O (pH 3)
Inj. vol.: 10 μL (1 g/L)

Preparation

10 mM HCOOH+NH3 in H2O (pH 7)
Inj. vol.: 2 μL (20 g/L)

Load
40 μg

Duloxetine

15

Time (min)Time (min)

Load
10 μg

15
20
Time (min)■ Comparison of the number of peaks (Basic drugs)[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: 25 mM Phosphate buffer
A/B, 5/95-75/25-75/25 (0-30-40 min)Flow rate: 0.2 mL/min; Temp.: 40°C; Detection: UV 240 nm
Inj. vol.: upper: 0.5 μL, lower: 5 μL; Sample: Homochlorcyclizine

010

Purity check:
Before purification 98.2%
After purification>99.9%

Duloxetine

Duloxetine
H
N

O
S

5

10
Time (min)

15

5

10
Time (min)■ Preparative purification (Basic drugs)[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 4.6 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: Aqueous solution
A/B, Gradient elution
Flow rate: 1 mL/min; Temp.: 40°C; Detection: UV 230 nm
Sample: Duloxetine

15(pKa 8.1)

The use of an alkaline eluent provides the following
advantages in basic compounds:- Load increases and preparation efficiency is improved- Capable of preparation without counterion- Improvement in the separation of the degradation productor impurities.

6L-column3

A 100% aqueous eluent is available for stable analysis.
The deterioration of the silica-based column is promoted when used for a 100% aqueous eluent for a long period of time.
Since the packing materials for L-column3 have very high chemical stability, the column is stable for long periods of time evenwhen used for the 100% aqueous eluent.

Stability of N (Pyridoxine) (%)

120

L-column3

L-column3

1

2

100

3

0 Hour80

250 HourL-column2

60

0

2

4
6
Time (min)

40
0

50

100
150
Time (Hour)

200

250

L-column2

8

10

1
2

■ Durability test

3

0 Hour[Durability test conditions]
Column: C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: 25 mM Phosphate buffer pH 7
Flow rate: 0.2 mL/min; Temp.: 50°C

250 Hour- N : Number of theoretical plates

0

2

4
6
Time (min)810■ Vitamin B6[Analytical conditions]
Column: C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: 25 mM Phosphate buffer pH 7
Flow rate: 0.2 mL/min; Temp.: 40°C; Detection: UV 320 nm
Inj. vol.: 1 μL
Sample: 1. Pyridoxamine; 2. Pyridoxal; 3. Pyridoxine

Application data
Gradient elution

L-column3 C8
1

A: CH3CN, B: 25 mM Phosphate buffer pH 7, A/B, 0/100-10/90 (0-15 min)
7
1
2

23 4
5

6

7

4

6

3

5

10
8

L-column3 C18

9Isocratic elution
25 mM Phosphate buffer pH 7
23

1

4

1

5
6

0

5

10
Time (min)415■ Antihistamine[Analytical conditions]
Column: 5 μm; Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (45/55)
Flow rate: 1 mL/min; Temp.: 40°C; Detection: UV 220 nm
Inj. vol.: 1 μL
Sample: 1. Chlorpheniramine; 2. Diphenhydramine; 3. Diphenylpyraline
4. Homochlorcyclizine; 5. Hydroxyzine; 6. Clemastine
7. Promethazine

7

2

7

20

0

5

Time (min)

7
6

10

■ Nucleobase[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 4.6 mm I.D., 150 mm L.
Flow rate: 1 mL/min; Temp.: 40°C; Detection: UV 260 nm
Inj. vol.: upper: 2 μL (50 mg/L), lower: 5 μL (10 mg/L)
Sample: 1. Cytosine; 2. Uracil; 3. Cytidine; 4. Guanine; 5. Uridine
6. Thymine; 7. Adenine; 8. Guanosine; 9. Thymidine
10. Adenosine

15

L-column3

Application data
Alkaline eluent

Alkaline eluent

A: 5 mM NH3 in CH3OH
B: 5 mM NH3 in H2O

A: CH3CN
B: 5 mM NH3 in H2O

Neutral eluent *Acidic eluent

A: 5 mM CH3COONH4 in CH3OH
B: 5 mM CH3COONH4 in H2O

A: CH3CN
B: 5 mM HCOOH in H2O* Simultaneous test method for pesticides by
the LC/MS (agricultural products)

0

5

10

15
Time (min)

20

25

30

0

5

10

15

Time (min)■ Agricultural chemicals■ Quinolones[Analytical conditions]
Column: L-column3 C18, 3 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A/B, 15/85-40/60-40/60-50/50-55/45-95/5-95/5(0-1-3.5-6-8-17.5-30 min)Flow rate: 0.2 mL/min; Temp.: 40°C; Detection: ESI-MS/MS (+)
Inj. vol.: 5 μL (50 μg/L)[Analytical conditions]
Column: L-column3 C18, 3 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A/B, 1/99-60/40(0-15 min)Flow rate: 0.3 mL/min; Temp.: 40°C; Detection: ESI-MS/MS (+)
Inj. vol.: 1 μL (50 μg/L)

2
4

pH 11

Alkaline eluent
1

5

3

3

A: CH3CN/5 mM NH3 (95/5)
B: 5 mM NH3 in H2O
1

6

2
2

pH 7

4

1

5

3
6

pH 2

12

Neutral eluent *6

5* Department of Food Safety, Standards and
Evaluation Division Notification 0306 No. 4 andDepartment of Food Safety, Standards Bureau,
Inspection Division Notification 0306 No. 2

3

0

5

3

A: CH3CN/2 mM HCOONH4+50 mM HCOOH (95/5)
B: 2 mM HCOONH4+50 mM HCOOH in H2O

4

10

15

20

25

0

[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 4.6 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: 25 mM Phosphate buffer
A/B, 5/95-50/50 (0-25 min)Flow rate: 1 mL/min; Temp.: 40°C; Detection: UV 230 nm
Inj. vol.: 1 μL (250 mg/L)
Sample: 1. Famotidine; 2. Cimetidine; 3. Ranitidine; 4. Nizatidine
5. Lafutidine; 6. Roxatidine

2

8

12

Time (min)Time (min)■ H2 blocker

41■ Okadaic acids[Analytical conditions]
Column: L-column3 C18, 3 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A/B, 60/40-60/40-100/0-100/0(0-2.5-7.5-12.5 min)Flow rate: 0.2 mL/min; Temp.: 40°C; Detection: ESI-MS/MS (-)
Inj. vol.: 5 μL
Sample: 1. Okadaic acid; 2. Dinophysistoxin-2; 3. Dinophysistoxin-1

8

L-column3

UHPLC column
UHPLC (ultra-high performance liquid chromatography) is liquid chromatography provided at a higher speed with higher separation byusing a column with fine particle packing materials in a particle size of about 2 μm. L-column3 with a wide selection range of pH values
can select analytical conditions of detection sensitivity, peak shape, and elution order based on the intended purpose.

5 μm

2 μm

20

tR(4) : 2.6
N(4) : 17600

4
1

Analysis time: 1/2

2

Eluent: 1/5

Height equivalent to a theoretical plate(Naphthalene, μm)

Size: 2.1 mm I.D., 100 mm L.
Flow rate: 0.4 mL/min
Inj. vol.: 0.5 μL

Size: 4.6 mm I.D., 150 mm L.
Flow rate: 1 mL/min
Inj. vol.: 1 μL

3

tR(4) : 6.2
N(4) : 17100

1

15

5 μm

10

3 μm

5

2 μm

Optimum linear velocity range

4
0

2

１

4
２
３
Flow velocity (mm/sec)

5

6

3

2

0

0

6

4
Time (min)082Time (min)The decrease in particle size increases the linear velocity ofthe mobile phase (eluent) with height equivalent to atheoretical plate, and the change of that value is small within
the wide range of the linear velocity. Therefore, UHPLC
enables the analyses at a higher speed. In addition, thedecrease of the inner diameter of the column and the length
enables a reduction in the amount of eluent use.

4

■ Standerd test
[Analytical conditions]
Column: L-column3 C18
Eluent: CH3CN/H2O (60/40); Temp.: 40°C; Detection: UV 254 nm
Sample: 1. Uracil; 2. Benzene; 3. Toluene; 4. Naphthalene

L-column series (2 μm):
- Optimum flow rate range of inner diameter 2.1 mm:
approx. 0.4 mL/min- Optimum flow rate range of inner diameter 3.0 mm:
approx. 0.8 mL/min- tR: Retention time (min), N : Number of theoretical plates

Acidic eluent

Alkaline eluent
5 mM NH3 in H2O

5 mM HCOOH in H2O

Acidic eluent
1

Alkaline eluent

Neutral eluent

5 mM HCOOH in H2O

5 mM CH3COONH4 in H2O

5 mM NH3 in H2O
3

2

3
1
4

4

2
3

0

1

2
3
Time (min)

3

4

5

0

1

2
3
Time (min)■ Antidepressants (SSRI)[Analytical conditions]
Column: L-column3 C18, 2 μm; Size: 2.1 mm I.D., 100 mm L.
Eluent: A: CH3CN, B: Aqueous solution
A/B, 10/90-70/30 (0-5 min)Flow rate: 0.6 mL/min; Temp.: 40°C; Detection: ESI-MS/MS (+)
Inj. vol.: 5 μL (0.1 mg/L)
Sample: 1. Citalopram; 2. Paroxetine; 3. Duloxetine; 4. Fluoxetine

9

2

1
2
3

2

1

1

4

5

0

4
Time (min)804Time (min)80■ H2 blocker[Analytical conditions]
Column: L-column3 C18, 2 μm; Size: 2.1 mm I.D., 100 mm L.
Eluent: A: CH3CN, B: Aqueous solution
A/B, 0/100-50/50 (0-10 min)Flow rate: 0.3 mL/min; Temp.: 40°C; Detection: ESI-MS/MS (+)
Inj. vol.: 0.5 μL (0.1 mg/L)
Sample: 1. Famotidine; 2. Ranitidine; 3. Cimetidine

4Time (min)8L-column3

Metal-free column
The performance of packing materials is most important in the evaluation of metal-free column. Since the packing materials for the
L-column series suppress the influence of metal impurities, the peak of the compounds that are easily subjected to metal coordination
can be sharply detected. Furthermore, since the L-column3 metal-free column can be used in a wide range of pH values, animprovement in sensitivity and separation behavior can be expected.
Acidic eluent

Acidic eluent

5 mM HCOOH in H2O

Metal-free column

SUS column

5 mM HCOOH in H2O

Metal-free column

SUS column

2

S/N : 269
4

2
4

3

4

5

2

3

Time (min)

5

5

1

3

Time (min)

6
3

3

1

2
1

2

S/N : 72

S/N : 460

S/N : 83

6

4

5

2

6
Time (min)4■ Antibiotics (tetracycline)

1

34

8

Alkaline eluent[Analytical conditions]
Column: L-column3 C18, 3 μm; Size: 2.0 mm or 2.1 mm I.D., 50 mm L.
Eluent: A: CH3CN, B: 5 mM HCOOH in H2O
A/B, 1/99-60/40 (0-5 min)Flow rate: 0.2 mL/min; Temp.: 15°C; Detection: ESI-MS/MS (+)
Inj. vol.: 1 μL (50 μg/L)
Sample: 1. Oxytetracycline (OTC); 2. Tetracycline (TC)
3. Chlortetracycline( CTC); 4. Doxycycline (DC)

10

2

4

6
Time (min)

4

8

10

8

10

5 mM NH3 in H2O

Metal-free column

SUS column
S/N : 591

2

3

S/N : 642

3

6

2

6

1

2

1
4

- S/N : Signal to noise ratio
4
OH

OH
H O
O
OH

O

OH

O

O

H

O

O
OH

O

NH2
H
Cl HO CH3OHH
H N CH3
H3C

NH2
HCl HO CH3OHH
H N CH3
H3C

2

4

6
Time (min)

8

10

2

4

6
Time (min)■ Modified peptides

Keto-enol tautomerism of CTC
As the antibiotics of tetracycline base coordinates* with themetal ions at the β-diketone location in the structure, they
are strongly influenced by the metal.
The tetracycline has keto-enol tautomerism, and the peakleading of CTC and DC occurs. This change can besuppressed by setting the column temperature low.
* Quoted from Standard Methods of Analysis in Food Safety Regulation, AnimalDrugs and Feed Additives, Part 2003

[Analytical conditions]
Column: L-column3 C18, 3 μm; Size: 2.0 mm or 2.1 mm I.D., 50 mm L.
Eluent: A: CH3CN, B: Aqueous solution
A/B, 2/98-40/60 (0-10 min)Flow rate: 0.2 mL/min; Temp.: 40°C; Detection: ESI-MS/MS (+)
Inj. vol.: 1 μL (1 pmol/μL 0.1%HCOOH in H2O)
Sample: 1. Oxidized ACTH (1-10)
Oxide peptide
2. Glycosylated EPO (117-131)
Glycosylated peptide
Phosphorylated peptide
3. Phosphorylated AngiotensinⅡ
Nitrated peptide
4. Nitrated AngiotensinⅠ
Methylated peptide
5. Methylated Substance P
6. Acetylated Calcitonin (15-29)
Acetylated peptide- S/N : Signal to noise ratio* * * * * * * * * * * * ** * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *＜Precautions for use＞
-In order to sufficiently achieve the potential performance of a metal-free column, change the materials of tubings and needles tonon-metal materials for use in a metal-free system environment.
- Solvent that degrades PEEK cannot be used.
- The metal-free column has withstand pressure equivalent to that of general-purpose stainless-steel columns. Determine the columnpressure in reference to the following maximum pressure as a guideline.
Column Length Particle size:

5 μm

3 μm

2 μm

50 mm

10 MPa 20 MPa 40 MPa

100 mm15 MPa 25 MPa 60 MPa

150 mm15 MPa 25 MPa 80 MPa

250 mm20 MPa 30 MPa-

The hardware made of a PEEK lined stainless-steel tube
installed with PEEK frits is designed so that it can be used up
to pH 12.

PEEK
Stainlesssteel tube

PEEK lined
stainless-steel
tube

Packing materials (L-column3)
PEEK frit10L-column3

Technical report■ Degradation mechanism of silica-based columns by alkaline
solution
Packing materials using silica as the base material have many
types of bonded phases and particle sizes. The columns
packed with them are general purpose. However, in an alkaline
solution, hydroxide ion dissolves silica (Step 1). Furthermore,
the vacant space produced by the dissolution of silica at the topof the column significantly deteriorates the performance of thecolumn (Step 2). Therefore, the pH range of an eluent is limited.
Bonded phase(C18, etc...)Hydroxide ion
originated fromalkaline solution

Step 1End-capping group■ Advantage of using alkaline eluent“Capable of analysis when dissociaton is suppressed”
An ionic compound has the same concentration under
dissociation and non-dissociation conditions when pH is thesame as pKa. In propranolol as a basic drug, many propranolol
ions exist at a pH lower than 9.5, and many propranolol
molecules exist at a pH higher than 9.5. The retention by
propranolol ions is weak, and the retention by propranolol
molecules is strong.

Si

CH3
O

Si

Si

O

O
O

Si

Step 2

Si
O

O

O

Si

Low

OHO
O
Si

O
Si

Liquid pumping direction

Column inlet
side
Void occur
at inlet

Silicadissolve
gradually

N
OH H

CH3

Propranolol

pH 9.5Retention time (short)

O

O
Si

Si

O

Propranolol ions

O

OH-

CH3

N CH3
2+
OH H

High

Retention time (long)Since general C18 silics-based columns can only analyze by
propranolol ions, retention should be enhanced by adding an
ion-pair reagent (Fig. 3 (1)).
Because L-column3 with a wide range of pH values use cananalyze basic compounds under the condition of suppresseddissociation, sufficient retention can be obtained in a simple
eluent composition (Fig. 3 (2)).
Eluent: CH3CN/20 mM H3PO4 pH 2

(1)

CH3CN/20 mM H3PO4+
5 mM Sodium Hexanesulfonate

Packing
materials
05Time (min)Fig. 1 Image of silica dissolved by alkaline solution

Usable pH range of general columns

Stability of N (Naphthalene) (%)

5

(2)
pH 11

4
3

1
0

2

pH 9.5

(2)
pH 7

(2)
pH 2

2

50

Propranolol
4

6

8

10

12

0
(pH)

Usable pH range of L-column3

L-column3

100

(2)

General columns

80

pH 11

60

pH 7

L-column2

pH 2
40

0
0

50

100
150
Time (Hour)

200

250

Fig. 2 Durability test of alkaline eluent (pH 12)5Time (min)10[Analytical conditions]
Column: L-column3 C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: 25 mM Phosphate buffer
A/B, 50/50-70/30 (0-15 min)Flow rate: 0.2 mL/min; Temp.: 40°C; Detection: UV 255 nm
Inj. vol.: 1 μL

[Durability test conditions]
Column: C18, 5 μm; Size: 2.1 mm I.D., 150 mm L.
Eluent: CH3OH/54 mM TEA in H2O (10/90)
Flow rate: 0.2 mL/min; Temp.: 50°C

11

100

Propranolol ions

Abundance ratio (%)

6
Retention time (min)■ Alkaline resistance of L-column3
Recently, many manufacturers sell columns usable for an
alkaline eluent. While L-column2 is usable for pH 2 to pH 9,
L-column3 became usable for pH 1 to pH 12 (Fig. 2). The
columns with durability in a wide range of pH values are very
advantageous in the development of methods of analysis andfrom the economic viewpoint. The high durability is an importantfactor in the selection of columns.

120

10

Fig. 3 Abundance ratio and retention time due to pH change(propranolol)

http://www.cerij.or.jp

L-column3

Technical report“Changing separation behavior of ionic compounds bysimultaneous multicomponent analysis”
Changing the pH of an eluent changes the retention behavior of
the ionic compounds.
Because L-column3 is usable within the range of pH 1 to pH 12,
the pH of the eluent can be changed significantly.
Changing the pH enables an improvement in separation.

■ Precautions when using an alkaline eluent
Even when using some chemically stable columns, using theeluent with high pH is very severe conditions for the column.
The selection of an eluent with the pH matching the purpose of
the analysis is recommended. Strictly follow the operation
manual for columns when using an eluent with allowable pH.
Even allowable pH promotes deterioration of the performanceof the column in the following cases:“Improvement of peak intensity”
In the LC/MS often used for trace analysis, peak intensity isimportant. In the analysis of a basic compounds, higher pH
values of an eluent enable the acquisition of sufficient retention
and peak intensity. This is an advantage in the use of analkaline eluent.
Fig. 4 shows the peak area ratio assuming the peak area to be“1” when formic acid is used as an eluent. The peak area ofibuprofen, an acidic drug, changes little even if the pH of theeluent changes. On the other hand, an increase in the pH of theeluent increases the peak area of amitriptyline.

- Ratio of organic solvent is low.
- High column temperature.
- Eluent is added in a high concentration.

The selection of the pH value of the eluent matching the
purpose of the analysis is recommended.

5 mM HCO3NH45 mM CH3COONH4
5 mM HCOONH4■ Amitriptyline (pKa 9.4)
■ Ibuprofen (pKa 4.5)

0.1% HCOOH
1

2

3
4
Area ratio

5[Analytical conditions]
Column: L-column2 ODS, 3 μm; Size: 2.1 mm I.D., 50 mm L.
Eluent: A: CH3CN, B: Aqueous solution, A/B, Gradient elution
Temp.: 40°C; Inj. vol.: 5 μL

Fig. 4 Comparison of peak area ratio

6

Parts of the LC or LC/MS to be used may be replaced withthose resistant to alkaline. Ask the manufacturer for further
information. After use, wash the column and the flow channel
with an eluent not containing additives.

<Supplement>
The method for preparing an eluent in this catalog is asfollows:
Select the grade of reagent (for HPLC) based on the
purpose of the analysis. Chemical change, pH change, androtting by the microorganism may occur after the
preparation. Completely use the eluent within one or twodays.
■ 0.1% formic acid solution
Formic acid 1 mL
Add water to prepare a total volume of 1000 mL■ 5 mM ammonium formate solution
1 mol/L ammonium formate solution 5 mL
Add water to prepare a total volume of 1000 mL■ 5 mM ammonium acetate solution
1 mol/L ammonium acetate solution 5 mL
Add water to prepare a total volume of 1000 mL■ 5 mM ammonium hydrogen carbonate ( ammonium
bicarbonate) solution
Ammonium hydrogen carbonate (M.W = 79.06) 0.40 g
Add water to prepare a total volume of 1000 mL■ 5 mM ammonium (ammonium hydroxide) solution
28% ammonia water 334 μL
Add water to prepare a total volume of 1000 mL■ 25 mM Phosphate buffer pH 2
Phosphoric acid (85%) 1020 μL (equivalent to 15 mM)
Sodium dihydrogen phosphate dihydrate 1.56 g(equivalent to 10 mM)
Add water to prepare a total volume of 1000 mL■ 25 mM Phosphate buffer pH 7
Potassium dihydrogen phosphate 1.36 g (equivalent to 10
mM)
Disodium hydrogen phosphate nonahydrate 2.13 g(equivalent to 15 mM)
Add water to prepare a total volume of 1000 mL■ 25 mM Phosphate buffer pH 11
Dipotassium hydrogen phosphate 4.17 g (equivalent to 24
mM)
Tripotassium phosphate 0.23 g (equivalent to 1 mM)
Add water to prepare a total volume of 1000 mL

12
http://www.cerij.or.jp

L-column3

Line upPacking materials: L-column3 C18 (USP category: L 1) (Octadecylsilanized silica gel for liquid chromatography)
LengthParticle size

2 μm

2.1 mm

3.0 mm
3 μm

10 mm

20 mm

30 mm

50 mm

75 mm

100 mm

150 mmCat.No.

813780813770813630813140813640813170813020Price (JPY)58,00063,00070,00072,00074,00076,00086,000Cat.No.

823650823490823600823330Price (JPY)75,00077,00079,00081,000Inner diameter

1.0 mmCat.No.

811740811610Price (JPY)

62,00067,0001.5 mmCat.No.

2.1 mmCat.No.

3.0 mm81116081101081163081114081164081117081102081122082165082149082160082133082126082132057,00057,00062,00062,00067,00084,000Cat.No.

821060821150821460821180821070821080Price (JPY)54,00054,00060,00060,00070,00087,0005 μm

81178081177039,00044,000Cat.No.
Price (JPY)

4.6 mm811130250 mmCat.No.

812740812610Price (JPY)59,00065,0001.5 mmCat.No.

8121308121608120102.1 mmCat.No.

8121408121708120208122203.0 mmCat.No.

8223308222608223201.0 mm

812630Price (JPY)
4.0 mm
4.6 mm

10.0 mm54,00054,00060,000Cat.No.

64,00077,000822040822310Cat.No.

822060822150822180822070822080Price (JPY)50,00050,00055,00065,00082,000Cat.No.

842510842100Price (JPY)

220,000

280,000

100 mm

150 mm

250 mm811161811011- Connection type: 1/16” Waters.

Packing materials: L-column3 C8 (USP category: L 7) (Octylsilanized silica gel for liquid chromatography)
LengthParticle size

3 μm

Inner diameter

10 mm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

5 μm

50 mm

75 mm

81113181178181177181163181114181164181117181102181122182165182149182160182133182126182132157,00057,00062,00062,00067,00084,000821061821151821461

821181821071821081Price (JPY)54,00054,00060,00087,0001.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.
Price (JPY)39,00044,00081263160,00070,00081213181216181201181214181217181202181222182233182226182232164,00077,00054,00054,00060,0004.0 mmCat.No.

822041822311

4.6 mmCat.No.

822061822151822181822071822081Price (JPY)50,00050,00055,00065,00082,000- Connection type: 1/16” Waters.

13

30 mm

Cat.No.

Price (JPY)
4.6 mm20 mm

L-column3

Line upGuard column (cartridge type)
Packing materials

Particle size

L-column3 C18

5 μm
5 μm

L-column3 C8

HolderDirect typeNon-direct type

Inner diameter

LengthSpecification

Cat.No.

Price (JPY)

2.0 mm

5 mm

Cartridge (3 pcs)

85233032,0004.6 mm

10 mm

Cartridge (3 pcs)

85205031,0002.0 mm

5 mm

Cartridge (3 pcs)

852331

32,0004.6 mm

10 mm

Cartridge (3 pcs)

852051

31,000For 2.0 mm I.D.

(1 pc)

65133222,000For 4.6 mm I.D.

(1 pc)

65105222,000For 2.0 mm I.D.

(1 pc)

65233222,000For 4.6 mm I.D.

(1 pc)

65205222,000- Connection type: 1/16” Waters.
- Select a guard column with the same inner diameter as that of the analytical column or a smaller inner diameter than that of the analytical column.
- The direct connection type of dedicated holder can be directly connected to the analytical column. The indirect connection type requires tubing and connectors to connect the holder
and the analytical column.

Holder

Cartridge

Direct typeNon-direct type

Metal-free column
LengthPacking materials

Particle size

Inner diameter

L-column3 C18

2 μm

2.0 mm

3 μm
5 μm

L-column3 C8

2.0 mm

2.0 mm3 μm

2.0 mm5 μm

2.0 mm

50 mm

100 mm

150 mmCat.No.

863140863170863020Price (JPY)

102,000

106,000

116,000

250 mmCat.No.

861140861170861020861220Price (JPY)87,00092,00097,000114,000

Cat.No.

862140862170862020862220Price (JPY)84,00090,00094,000107,000

Cat.No.

861141861171861021861221Price (JPY)87,00092,00097,000114,000

Cat.No.

862141862171862021862221Price (JPY)84,00090,00094,000107,000- Connection type: 1/16” Waters.

Pre-column filter
Specification

Cat.No.

Price (JPY)

Filter (1 pc), holder W+W (1 pc)

Connection type: Column side, Waters type; LC tubing side, Waters type65300218,000Filter (1 pc), holder W+U (1 pc)

Connection type: Column side, Waters type; LC tubing side, UPLC® type65300418,000Filter (5 pcs)

For replacement

653003

9,800- The precolumn filter prevents the entry of insoluble compounds and protects the analytical column..

■ About connection type (joint)
The connection type (joint and thread type) of column is divided
into several types. The connection type is distinguished bydifferent tubing lengths at the end of the ferule (below diagram“a”). Always connect by using the same connection type for the
LC tubing and the column or by using tough connectors to avoid
the generation of dead volume.

Column end-fitting

1/16”O.D.

a
The connection of L-column3 is the Waters type (unified finethread No.10-32UNF, outer diameter of tubing 1/16”, and thelength of tubing “a” at the end of ferule approx. 3.5 mm).

Tube fittingaNo.10-32UNF

14

L-column Series

Reference: About L-column series
The L-column series appeared in 1990 and evolved corresponding to user needs with the concept of low adsorption, high durability,
high resolution, and high reproducibility. We earned a very favorable reputation because of our untiring efforts for performance andquality and a consistent stable supply for many years.
Packing materials

USP
category

L-column3 C18

L1

L-column3 C8
L-column2 ODS

Particle Size(μm)

Pore Size(Å)

Micro column

Semi-micro andgeneral-purpose
columnSemi
preparation
columnInner diameter
less than 1 mm

Inner diameter 1 mm ormore and less than 10
mm

Inner diameter
10 mm or more

-

○

○

○

Metal-freecolumn2, 3, 5

120

L7

3, 5

120

-

○

-

○

L1

2, 3, 5

120

○

○

○

○

L-column2 C8

L7

3, 5

120

○

○

-

○

L-column2 C6-Phenyl

L 11

3, 5

120

○

○

-

○

L-column ODS

L1

3, 5

120

○

○

○

-

L-column ODS-P

L1

5

300

○

○

-

-

L-column C8

L7

5

120

○

○

-

-

Classification by inner diameter cited from JIS K 0124:2011

General commercial column

L-column series
L-column:
High temperature gas phase end capping
L-column2: Advanced end-capping

L-column3: Perfect stable end-capping

Interaction withbonded phase

Interaction withbonded phaseanalyteanalyteFurther
evolvedEnd-capping
layer
Dissolutionof silica

Interaction with Interaction withsilanol group metal impurities

End-capping layer

End-capping layer

Fully porous high purity silica

PCS silica (fully porous high purity silica)

Interaction with silanol group
Interaction with metal impurities

Bonding density: Low
Adsorptivity: High (tailing)
Durability: Low
Difference between lots: Large
Inert level of packing
materials

Eliminated to the utmost limit

Bonding density: High
Adsorptivity: Low (right and left symmetrical peak)
Durability: High
Difference between lots: Small

Low

General commercial column

High

<

L-column<

L-column2≤

L-column3

The silica-based reversed phase columns are created by modifying the bonded phases on the surface of the silica and end-capping
silanol groups. Although end-capping is performed using different methods depending on manufacturers, the complete elimination of
the silanol groups is difficult. Therefore, the peak of the analyte is subjected to tailing because of the interaction with the silanolgroups or metal impurities.
The L-column series eliminated the secondary interaction (interaction with silanol groups and metal impurities) of the silica basereversed phase column to the utmost limit by applying unique end-capping technology and pursued the separation mechanism of an
original column. Since the L-column ODS entered the stage in 1990, the columns have continually evolved.

15

L-column Series

Reference: About L-column series
The L-column series may present a different separation pattern in a simultaneous multicomponent analysis even when using the same
C18 (ODS) column because the manufacturing method for the packing materials is different.

L-column

L-column2

L-column3

Base material

Fully porous high purity silica

Fully porous high purity silica

PCS silica (Fully porous high purity silica)

End-cappingHigh temperature gas phase end-capping

Advanced end-capping

Perfect stable end-capping

pH 2 - pH 9

pH 1 - pH 12

Usable pH range * pH 2 - pH 9

Features

You can use without worry because thishas been stably supplied since 1990.

The influence of the silanol group was
eliminated to the utmost limit.
It offers an abundant lineup, such as thephenyl column, micro column, and nanocolumn with inner diameters less than 1
mm.
Metal free columns (glass lining) are alsoavailable.

When comparing the L-column and the
L-column2, retention is weaker, andstereoselectivity is a little lower.
The inert level of packing material is high,and it has low adsorptivity equivalent to thatof the L-column2.
Not only the alkaline resistance but also the
durability in an eluent with high water ratio
were dramatically improved.Metal freecolumns (PEEK lining) are also available.

Characteristic
test

Cat.No.622070
Column: C18, 5 μm
Size: 4.6 mm I.D., 150 mm L.

Cat.No.722070

Cat.No.822070

323

1. Uracil
2. Caffeine
3. Phenol
4. Butylbenzene
5. o-Terphenyl
6. Amylbenzene
7. Triphenylene

321

Hydrogen bond property : 0.52
Hydrophobicity: 1.47
Stereoselectivity: 1.552
1

Hydrogen bond property : 0.46
Hydrophobicity: 1.46
Stereoselectivity: 1.58756

Adsorptivity(SSRI)

5

5 6

15

20

Cat.No.622070
Column: C18, 5 μm
Size: 4.6 mm I.D., 150 mm L.

0

5

2

10
Time (min)15

20

Cat.No.722070

0

5

56

2

0

5

20

S(3) : 1.24

3

3

1
2

1

20

15

Cat.No.822070

3

15

10
Time (min)S(3) : 1.38

1

5

4

10
Time (min)S(3) : 1.58

0

7

4

10
Time (min)1. Paroxetine
2. Citalopram
3. Fluoxetine

Hydrogen bond property : 0.46
Hydrophobicity: 1.45
Stereoselectivity: 1.35

7

4

0

1

10
Time (min)

15

20

0

5

10
Time (min)15

20* In case the bonded phase is C18 (ODS)

The characteristic test evaluates the following values:<Hydrogen bond property> k (Caffeine) / k (Phenol)
This is the value representing the influence of the hydrogen bond of the silanol group of packing materials and the samples asrepresented by the ratio of the retention factors of caffeine with high hydrogen bond property and phenol with low hydrogen bondproperty. The larger the value of "hydrogen bond property", the hydrogen bonds are easy to occur between analyte and packing
materials.
<Hydrophobicity> k (Amylbenzene) / k (Butylbenzene)
This is the value representing the magnitude of the retention force of packing materials as represented by the ratio of the retention
factors of amyl benzene and butyl benzene. The larger the value of hydrophobicity, the higher the hydrophobicity of the packing
materials and the longer the retention time.
<Stereoselectivity> k (Triphenylene) / k (o-Terphenyl)
This is the value representing the plane recognition ability as represented by the ratio of the retention factors of triphenylene with aplane structure and o-terphenyl with a three-dimensional structure. The larger the value of stereoselectivity, the stronger the retentionof a compound with a plane structure.
- k: Retention factor, S : Symmetry factor

16L-column Series

Reference: Line up L-column, L-column2
L-column2
LengthPacking materials

Particle size

L-column2 ODS(USP category: L 1)

2 μm

Inner diameter
2.1 mmCat.No.

30 mm

50 mm

75 mm

100 mm

150 mm

713630713140713640713170713020723650723490723600723330250 mmPrice (JPY)
3.0 mmCat.No.
Price (JPY)

3 μm

2.1 mmCat.No.

7116307111407116407111707110207112203.0 mmCat.No.

721650721490721600721330721260721320721150721460721180721070721080Price (JPY)
4.6 mmCat.No.
Price (JPY)5 μm

1.5 mm
2.1 mmCat.No.

712130712160712010Cat.No.

712140712170712020712220722150722180722070722080Price (JPY)
4.6 mmCat.No.
Price (JPY)

L-column2 C8(USP category: L 7)

3 μm

2.1 mmCat.No.

711631711141711641711171711021711221

3.0 mmCat.No.

721651721491721601721331721261721321721151721461

721181721071721081712141712171712021712221722151722181722071722081Price (JPY)
4.6 mmCat.No.
Price (JPY)

5 μm

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)

L-column2 C6-Phenyl(USP category: L 11)

3 μm

2.1 mmCat.No.

711636711146711646711176711026711226

3.0 mmCat.No.

721656721496721606721336721266721326721156721466721186721076721086712146712176712026712226722156722186722076722086

Price (JPY)
4.6 mmCat.No.
Price (JPY)

5 μm

2.1 mmCat.No.
Price(JPY)

4.6 mmCat.No.
Price (JPY)- Connection type: 1/16” Waters.

L-column2 Metal-free column
LengthPacking materials
L-column2 ODS(USP category: L 1)

Particle size

Inner diameter3 μm

2.0 mm5 μm

2.0 mmCat.No.

50 mm

100 mm

150 mm

250 mm731140731170731020731220732140732170732020732220Price (JPY)Cat.No.
Price (JPY)- L-column2 Metal-free column is made from the combination of a glass lined stainless steel tube and non-metallic frits.
- Connection type: 1/16” Waters.

17

L-column Series

Reference: Line up L-column, L-column2
L-column
LengthPacking materials

Particle size

L-column ODS(USP category: L 1)

3 μm

Inner diameter

30 mm

50 mm

75 mm

100 mm

150 mm

250 mm

2.1 mmCat.No.

6116306111406116406111706110206112203.0 mmCat.No.

621650621490621600621330621260621320621150621460621180621070621080612140612170612020612220622150622180622070622080612147612177612027612227622157622187622077622087612141612171612021612221622151622181622071622081Price (JPY)
4.6 mmCat.No.
Price (JPY)

5 μm

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)

L-column ODS-P(USP category: L 1)

5 μm

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)

L-column C8(USP category: L 7)

5 μm

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)- Connection type: 1/16” Waters.

Nano column / micro column with 0.075 mm I.D. - 0.3 mm I.D.
LengthParticle size: 2 μm
50 mm

Particle size: 3 μm
50 mm

150 mm

500 mm

50 mm

150 mm

711410711420711800712410712420711390711400711810712390712400711290711300712290712300711270711280712270712280150 mm

Packing materials

Inner diameter

L-column2 ODS(USP category: L 1)

0.075 mm

Cat.No.

0.1 mmCat.No.

0.2 mmCat.No.

7132907133000.3 mmCat.No.

713270713280Particle size: 5 μm

Price (JPY)
L-column ODS(USP category: L 1)

0.075 mm

Cat.No.

6114106114206124106124200.1 mmCat.No.

6113906114006123906124000.2 mmCat.No.

611290611300612290612300Cat.No.

6112706112806122706122800.3 mmPrice (JPY)- Connection type: Valco

Trap column (cartridge type)
Packing materials

Particle size

Inner diameter

LengthL-column2 ODS
L-column ODS

5 μm

0.3 mm5 μm

0.3 mmHolderSpecification

Cat.No.

5 mm

Cartridge (3 pcs)

7524505 mm

Cartridge (3 pcs)

652450(1 pc)

652452

For 0.3 mm I.D.

Price (JPY)- Connection type: 1/16” Waters.

Holder

Cartridge18

LC column catalog
L-column3
Ver. 02

Chemicals Evaluation andResearch Institute, Japan■ The product specifications are as of April 1, 2019. Please note that they may be changed without prior notice.
■ For questions about part prices or ordering, please contact the Chromatography Department of CERI or a nearby agency. Details areprovided on the page for the Chromatography Department of our website. Please use them as a reference.

http://www.cerij.or.jp
CERI Tokyo
Chromatography department

ISO
9001
Design,Development,Production
and supply of Column
for GC and HPLCe-mail chromato@ceri.jp

FAX +81-480-37-2521
1600 Shimotakano, Sugito-machi, Kitakatsushika-gun,
Saitama 345-0043, Japan

KANTO CHEMICAL CO., INC.
REAGENT DIVISION
2-1, Nihonbashi Muromachi 2-chome, Chuo-ku, Tokyo,
103-0022, Japan
TEL: +81-3-6214-1092
E-mail : kanto-61@kanto.co.jp
Website: https://www.kanto.co.jp©2019 CERI, Japan

2019/04