TECHNICALDATASHEETLight Producing Microorganisms

Caution: For Research Use. This product is intended for animal research only and not for use in humans. Not for human or animal therapeutic ordiagnostic use.

Staphylococcus aureus
S. aureus ATCC 12600 (Xen29)
Product No.: 119240
Material Provided: 1 Agar Plate
Storage Conditions: -80°C
In vitro Characteristics
Genetic Characteristics
Staphylococcus aureus Xen29 was derived fromthe parental strain S. aureus 12600, a pleural fluid
isolate, which is also designated as NCTC8532. S.
aureus Xen29 possesses a stable copy of themodified Photorhabdus luminescens luxABCDE
operon at a single integration site on the bacterialchromosome.
Growth Characteristics
S. aureus Xen29 grows well in various media
including Luria Bertani (LB), Brain Heart
Infusion (BHI), and Nutrient Broth (NB) at 37ºC
under ambient aeration. S. aureus Xen 29 may
also be grown selectively on medium containing
200 μg/ml kanamycin.
Colonial Morphology
On LB agar, S. aureus Xen29 appears as small(~1.5mm), cream-colored, opaque, smooth,circular colonies.
Virulence Factor
Hemolysis: β-hemolysis on TSA + 5% sheep
blood
Capsule: Serotype 3 (NCTC type strain).
DNAse: Positive.

119240-Xen29-Rev-2

NaCl: Tolerant via growth on Mannitol SaltsAgar.
Coagulase: Positive in 24hrs.
mecA: negative
Growth Curve
Log-phase growth can be achieved after 2 to 3
hours of subculture in LB broth at 37ºC, shaking
at 200rpm. An absorbance measurement at 600nm(against a LB blank) of 0.5 is roughly equivalent
8
to 1.44x10 cfu/ml of S. aureus Xen 29.

Biochemical Profile
A biochemical profile was obtained for S. aureus
Xen 29 using the api 20 STAPH system available
from bioMérieux.
Sugar Utilization
D-Glucose
D-Fructose
D-Mannose
MaltoseLactoseTrehalose
D-Mannitol+++++++Xylitol-Raffinose-Xylose-D-Melibiose
Sucrose++Other Tests
Nitrate Reduction
Alkaline Phosphatase
Voges Proskauer
α- methyl-D-glucosideN-acetyl-glucosamine
Arginine dihydrolase
UreaseNCCLS Macrodilution MIC/MBC+++++Resistant to:
Kanamycin

References
J Biomed Mater Res B Appl Biomater. July 2008
Microbes and Infection, Vol. 10, Issue 3, March 2008, 217-223
J Orthop Res. 2008 Jan; 26(1):96-105
J Biol Phys 2008 Vol. 33:61–66
Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794
Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794
Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com

MIC (μg/mL)
8.0
4.0
2.0
R up to 16
0.125
0.5

MBC (μg/mL)
32
8.0>16*n/a0.25>16Product Information

Kirby-Bauer Disk Diffusion Test

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AntibioticCeftriaxone
Ciprofloxacin
Erythromycin
Gentamicin
Penicillin G
Tetracycline* trailing endpoint.

Antibiotic Susceptibility
Disk Diffusion Data: Disk diffusion tests wereperformed according to methods outlined in theNCCLS Approved Standard M2-A7.

Sensitive to:Carbenicillin 100
Gentamicin 20
Penicillin G 10U
Vancomycin 30

MIC and MBC Data
MIC and MBC were determined using the macrodilution methods specified in the NCCLS
Approved Standard M7-A5.

Warranty
PerkinElmer warrants that cells will be viable
upon shipment from PerkinElmer for a period ofthirty days, provided they have been properly
stored and handled during this period.
Disclaimers
This product is sold for in vivo animal researchuse only and is not for use in any diagnostic
procedures. Excluding purchases by authorized
PerkinElmer distributors, this product is sold foruse by the original purchaser and is not for resale.
8. Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794
9. Photochem. Photobiol. Sci., 2006, 5, 31 – 38
10. Journal of Antimicrobial Chemotherapy, Apr. 2005, Vol. 55, No. 4,528-534
11. Antimicrobial Agents & Chemotherapy, June 2004 2283-2287
12. Antimicrobial Agents & Chemotherapy, June 2004, Vol. 48, No. 6,2283-2287
13. Infection & Immunity, Feb. 2003, Vol. 71, No. 2, 882-890
14. Antimicrobial Agents & Chemotherapy, Oct. 2003, Vol. 47, No. 10,3130-313

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119240-Xen29-Rev-2