TECHNICAL
DATA
SHEET

Bioware® Brite Cell Line

Research Use Only. Not for use in diagnostic procedures.

Bioware® Brite Cell Line BxPC3-Red-FLuc
Product No.: BW125058

Material ProvidedCells:2 x 1 mL frozen aliquots (BW125058V)

Format:1.0 x 106 cells / mL in 95% FBS, 5% DMSODESIGNATION

BxPC3-Red-FLuc

TissueParental Cell Line
Gene Transfer Vehicle
Bioluminescence In Vitro

Human: pancreatic adenocarcinoma
ATCC (CRL-1687)
Red-FLuc-Puro 3d generation lentivirus
At least 15,000 photons/cell/sec. Exact number will vary dependingon imaging and culturing conditions.

Recommended Media and FBS

RPMI 1640 ATCC Cat. No. 30-2001.
Supplement the above with 10% Hyclone Fetal Bovine Serum(FBS) GE HealthCare Cat. No. SH300071
Remove frozen cells from dry ice packaging and immediately place
cells at a temperature below -130° C, preferably in liquid nitrogen
vapor, until ready to use.
36 hours
When initially thawing, use T25 flask or 10cm plate. Cells should
be ready to expand within 3-7 days. Antibiotics can be used in themedia if desired after the initial thaw. (puromycin at 2ug/mL).

Recommended Storage Conditions

Average Doubling Time
Other Recommendations

Refer to Cell Culture Guidelines for more detailed instructions.

PerkinElmer, Inc.
940 Winter StreetWaltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our products, visit www.perkinelmer.com.
Copyright ©2015, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All othertrademarks are the property of their respective owners.
BW125058-Rev-5

The Features
Perkin Elmer Bioware® Brite cell line models offer researchers the ability to:Monitor early tumor development

Monitor tumor growth and metastases in vivo

Quantify tumor burden in the whole animal

Follow responses to therapeutic treatments non-invasively in longitudinal studies using thesame cohorts of mice

Murine Pathogen FreeAll Perkin Elmer cell lines are confirmed to be pathogen free by the IMPACT Profile I (PCR) at the
University of Missouri Research Animal Diagnostic and Investigative Laboratory.
Cell Line Stability
Cell may undergo genotypic changes resulting in reduced responsiveness over time in normal cellculture conditions. Genetic instability is a biological phenomenon that occurs in all stablytransfected cells. Therefore, it is recommended to prepare an adequate number of frozen stock atearly passages.
Product Warranty
PerkinElmer warrants that cells will be viable upon shipment from PerkinElmer for a period of thirtydays, provided they have been properly stored and handled during this period.
Human Pancreatic Cancer Cell Line: BxPC3-Red-FLuc
BxPC3-Red-FLuc is a luciferase expressing cell line which was stablytransfected with firefly luciferase gene from Luciola Italica (Red-FLuc). The cellline was established by transducing lentivirus containing Red-FLuc luciferase
under the control of human ubiquitin C promoter. These cells will serve as a newtool to detect drug efficacy in vitro and in vivo with high sensitivity.

Bioluminescence imageof BxPC3 Red-FLuc
subcutaneous tumor

BW125058-Rev-5

Growth Curve of BxPC3-Red-FLuc Cells
Figure 1. 5 x104 cells were plated on a 6cm plate. The total numbers of cells werecounted every 24 h using a Nexcelom automatic cell counter.

In Vitro BLI Signal Stability

week 1week 2week 3week 4
Figure 2. 5 x104 cells were plated per well in 24-well plates. Cells were incubated at 37 °C for recovery overnight and luciferase assay
was performed using the PerkinElmer IVIS® SpectrumCT. Each experiment was done in quadruplicates. The cells were maintained incontinuous culture over four weeks and weekly luciferase assay was performed. Bioluminescence data was analyzed using the Living
Image 4.0 software.

BW125058-Rev-5

Subcutaneous Tumor Growth in a Nu/nu Mouse

day1day9day18

day24

day40

Figure 3. 3 x 106 BxPC3-RedLuc cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse.
Tumor growth was monitored for luciferase expression using the PerkinElmer IVIS ® Spectrum at various time points. Mice wereimaged 10 minutes post i.p. injection of luciferin at 150mg/kg at various time points. The image above shows tumor growth from a
representative mouse.

For more information on our in vivo imaging agents, please visit our website: www.perkinelmer.com/bioware.
This product is sold for in vivo animal research use only and is not intended for any diagnostic use or procedures.
Excluding purchases by authorized PerkinElmer distributors, this product is sold for use by the original purchaser
and is not for resale. Unless otherwise agreed to in writing by PerkinElmer pursuant to a separate writtenagreement, no commercial use of this product is allowed. “Commercial use” means any and all uses of this productand/or its derivatives by a party for money or other consideration and may include without limitation: (1) productmanufacture; (2) providing services, information or data to another party for remuneration; and/or (3) resale ofthe product or its derivatives, whether or not such product or derivatives are resold for use in research.
Commercial use does not include the original purchaser providing the product to its contractor solely for use on
the original purchaser’s research; provided that all product materials are returned to the original purchaser
and/or destroyed by the contractor upon completion of such project.

BW125058-Rev-5