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Bioware® Brite Cell Line

Research Use Only. Not for use in diagnostic procedures.

Bioware® Brite Cell Line 4T1-Red-FLuc-GFP
Product No.: BW128090
We recommend using this line in Nude and SCID mouse models.
An immune response may occur in Balb/c mice.
Material ProvidedCells:2 x 1 mL frozen aliquots (BW128090V)

Format:1.0 x 10 cells / mL in 95% FBS, 5% DMSO

6DESIGNATION

4T1-Red-FLuc-GFP

TissueSource of Parental Line
Gene Transfer Vehicle
Bioluminescence In Vitro

Mouse: mammary gland; adenocarcinomaATCC (CRL-2539)
Red-FLuc-GFP 3d generation lentivirus
At least 15,000 photons/cell/sec. Exact number will varydepending on imaging and culturing conditions.
RPMI 1640 ATCC Cat. No. 30-2001.
Supplement the above with 10% Hyclone Fetal Bovine Serum(FBS) GE HealthCare Cat. No. SH300071
Remove frozen cells from dry ice packaging and immediately
place cells at a temperature below -130° C, preferably in liquid
nitrogen vapor, until ready to use.
14 hours
When initially thawing, use T25 flask or 10cm plate. Cells
should be ready to expand within 1-4 days. Please note there isNO antibiotic selection for this cell line.

Recommended Media and FBS

Recommended Storage Conditions

Average Doubling Time
Other Recommendations

Refer to Cell Culture Guidelines for more detailed
instructions.

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Copyright ©2015, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All othertrademarks are the property of their respective owners.
BW128090-Rev-5

The Features
Perkin Elmer Bioware® Ultra cell line models offer researchers the ability to:

Monitor early tumor development

Monitor tumor growth and metastases in vivo

Quantify tumor burden in the whole animal

Follow responses to therapeutic treatments non-invasively in longitudinal studies using thesame cohorts of mice
Perform in vitro and ex vivo analysis with GFPMurine Pathogen Free
All Perkin Elmer cell lines are confirmed to be pathogen free by the IMPACT Profile I (PCR) at theUniversity of Missouri Research Animal Diagnostic and Investigative Laboratory.
Cell Line Stability
Cell may undergo genotypic changes resulting in reduced responsiveness over time in normal cell
culture conditions. Genetic instability is a biological phenomenon that occurs in all stablytransfected cells. Therefore, it is recommended to prepare an adequate number of frozen stock atearly passages.
Product Warranty
PerkinElmer warrants that cells will be viable upon shipment from PerkinElmer for a period of thirtydays, provided they have been properly stored and handled during this period.
Mouse Mammary Gland Cancer Cell Line: 4T1-Red-FLuc-GFP
4T1-Red-FLuc-GFP is a luciferase expressing cell line which
was been stably transfected with the firefly luciferase gene fromLuciola Italica (Red-FLuc) fused to GFP protein. The cell line
was established by transducing lentivirus containing Red-FLuc
luciferase-T2A-GFP gene under the control of human ubiquitin Cpromoter. These cells will serve as a new tool to detect drug
efficacy in vitro and in vivo with high sensitivity in Nude andSCID mouse models.

Bioluminescence and GFP images of4T1 –Red-FLuc-GFP subcutaneoustumors in nude mice

BW128090-Rev-5

Growth Curve of 4T1-Red-FLuc-GFP Cells
Figure 1. 5 x104 cells were plated on 6cm plate and the total numbers of cells
were counted every 24 h using a Nexcelom automatic cell counter.

In Vitro BLI Signal Stability

week 1week 2week 3week 4

Figure 2. 5 x104 cells were plated per well in 24-well plates. Cells were incubated at 37 °C for recovery overnight and luciferase assay
was performed using the PerkinElmer IVIS® SpectrumCT. Each experiment was done in quadruplicates. The cells were maintained incontinuous culture over four weeks and weekly luciferase assay was performed. Bioluminescence data was analyzed using the Living
Image 4.0 software.

BW128090-Rev-5

Subcutaneous Tumor Growth in a Nu/nu Mouse

day1

day3

day6

day9

day12

Figure 3. 1 x 106 4T1-Red-FLuc-GFP cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse.
Tumor growth was monitored for luciferase expression using the PerkinElmer IVIS ® Spectrum at various time points. For luciferase
expression, mice were imaged 10 minutes post i.p. injection of luciferin at 150mg/kg at various time points. The image above shows
tumor growth from representative mouse.

For more information on our in vivo imaging agents, please visit our website: www.perkinelmer.com/bioware.
This product is sold for in vivo animal research use only and is not intended for any diagnostic use or procedures.
Excluding purchases by authorized PerkinElmer distributors, this product is sold for use by the original purchaser
and is not for resale. Unless otherwise agreed to in writing by PerkinElmer pursuant to a separate writtenagreement, no commercial use of this product is allowed. “Commercial use” means any and all uses of this productand/or its derivatives by a party for money or other consideration and may include without limitation: (1) productmanufacture; (2) providing services, information or data to another party for remuneration; and/or (3) resale ofthe product or its derivatives, whether or not such product or derivatives are resold for use in research.
Commercial use does not include the original purchaser providing the product to its contractor solely for use on
the original purchaser’s research; provided that all product materials are returned to the original purchaser
and/or destroyed by the contractor upon completion of such project.

BW128090-Rev-5