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DNASep™
Column Care Information

Catalog Number: DNA-99-3510

The ADS Biotec DNASep™ Column contains nonporous polystyrene-divinylbenzene (PS-DVB) copolymer beads
that are approximately 2µm in size and are alkylated with C-18 chains. The polymer packing is designed for theseparation of DNA. The column should be installed and used in accordance with the following instructions.
GENERAL
Each column is shipped with an attached label identifying column type, serial number, and flow direction. Besure this tag is kept on the column in case further details are needed about this specific column. Before installingthe column, the entire system should be flushed with the mobile phase to be used. The mobile phase should bepassed through a 0.2m filter and thoroughly degassed. Maximum pressure on this column should not exceed~2500-3000 psi. The typical detection system uses UV absorbance @ 260nm or 254 nm.
PHYSICAL CHARACTERISTICS
Dimensions: 4.6 mm X 50 mm
Packing Material: Alkylated PS-DVB beads, C18Ionic Form: NApH Range: 0-14
INSTALLATION-Before installing the column, thoroughly flush the HPLC system with 100 % Solution D (for 20 minutes @ 1.0mL/min). Best results will be obtained with the flow direction as indicated by the arrow on the column.
-Install the column and turn on the column oven to 45 °C (or other desired method conditions).
-Set Flow Rate to 1.0 mL/min.
-Equilibrate the column with 38% Buffer B for at least 20 min before analysis
COLUMN STORAGE
Be sure to flush DNASep™ column with 100% Solution D @ 1.0 mL/min for at least 30 minutes before removing
column for storage. The column must be stored in Solution D. Prior to storage, seal the column using thecolumn end-plugs before storing at room temperature.

Doc ID: 482681 rev 0
ADS Biotec and WAVE Optimized are registered trademarks. ©2017 ADS Biotec, Inc. All rights reserved.

www.adsbiotec.com
ADS Biotec Inc. | 7409 Irvington Road | Omaha, NE | USA 68122 | tel: 402- 800-3200
ADS Biotec Limited | 40 Watt Road | Hillington Park | Hillington, Glasgow | UK G52 4RY | +44 (0) 141 8928800
Registered in England and Wales. Registered office: Birketts LLP, 22 Station Road, Cambridge, CB1 2JD Company Registration No. 03257373 VAT Registration No. GB682479101

Send Purchase Orders: orders@adsbiotec.com
Technical Support: support@adsbiotec.com

DNASep™
Column Care Information

Catalog Number: DNA-99-3510

MOBILE PHASEADS Biotec columns were designed for optimal separation using ADS Biotec buffers, with more than 20 years’
manufacturing experience producing the highest quality HPLC buffers available, built upon the solid foundationof our flagship WAVE HPLC system.
2 types of ion-pair buffers are available, one using triethylammonium acetate (TEAA) suitable for common nucleic
acid separations and the second made using hexylammonium acetate (HAA) for challenging separations. Bothion-pairing reagents undergo quality testing prior to buffer manufacture to ensure clean baselines, optimalresolution, and separation consistency. Additionally, ADS Biotec offers custom buffer solutions ready to use
small or large volume in several packaging formats: bottles, drums and or other custom containers.
Item
Number553421553422553423553424553425
SP5890SP5892553422-L

Description
Mobile Phase for Nucleic Acid Analysis - Buffer A (0.1 M TEAA in water)
Mobile Phase for Nucleic Acid Analysis- Buffer B (0.1 M TEAA in 25 % Acetonitrile)
Column Wash Solution D (75 % acetonitrile)
Mobile Phase for Nucleic Acid Analysis - Buffer HA A (0.1 M HAA in 10% Acetonitrile)
Mobile Phase for Nucleic Acid Analysis - Buffer HA B (0.1 M HAA in 50% Acetonitrile)
2 M TEAA Solution
2 M HAA Solution
Custom Buffer Products
Custom Volume - Mobile Phase for Nucleic Acid Analysis - Buffer A (0.1 M TEAA inwater)
Custom Volume - Mobile Phase for Nucleic Acid Analysis- Buffer B (0.1 M TEAA in 25 %553423-L
553303-L
552303-LAcetonitrile)
Custom Volume - Column Wash Solution D (75 % Acetonitrile)
Custom Volume - 2 M TEAA Solution
Custom Volume - 2 M HAA Solution

553421-LSize
4 x 2.5 L
4 x 2.5 L
4 x 2.5 L
4 x 2.5 L
4 x 2.5 L
6 x 200 mL
6 x 200 mL
CustomerSpecifiedPackaging
and Volume

Doc ID: 482681 rev 0ADS Biotec and WAVE Optimized are registered trademarks. ©2017 ADS Biotec, Inc. All rights reserved.

www.adsbiotec.com
ADS Biotec Inc. | 7409 Irvington Road | Omaha, NE | USA 68122 | tel: 402- 800-3200
ADS Biotec Limited | 40 Watt Road | Hillington Park | Hillington, Glasgow | UK G52 4RY | +44 (0) 141 8928800
Registered in England and Wales. Registered office: Birketts LLP, 22 Station Road, Cambridge, CB1 2JD Company Registration No. 03257373 VAT Registration No. GB682479101

Send Purchase Orders: orders@adsbiotec.com
Technical Support: support@adsbiotec.com

DNASep™
Column Care Information

Catalog Number: DNA-99-3510

COLUMN REGENERATION PROCEDURE
To prolong the life of the DNASep™ Column it may be necessary to regenerate using Solution D (75%acetonitrile).
1.
2.
3.
4.
5.
6.

Reverse the flow direction of the column.
Set oven temperature to 80 °C.
Flush column with 100% Solution D @ 0.5 mL/min for 30 minutes.
Set oven temperature to 50 °C.
Reverse the flow direction of the column- back to normal direction. NOTE: Column will be HOT.
To prepare column for storage, continue with Solution D until column has cooled to 50 °C (or lower)
before removing.
7. To prepare column for a new sample, typically equilibrate first with 50% Buffer A, 50% Buffer B @0.5 mL/min for at least 30 minutes at the targeted oven temperature.
COLUMN PRECAUTIONS
No warranty exists for the column. Please note the following precautions in using the DNASep™ column:• Use only HPLC grade acetonitrile, <0.005 AU (UV absorbance) at 260 nm. ADS Biotec acetonitrile and/orADS Biotec Buffers are recommended.
• Use only HPLC grade water that has a resistivity of at least 18 MΩ purity with < 15 ppb T.O.C. (totalorganic carbon) and must not be autoclaved.
• Do not inject the following materials:➢ Bovine Serum Albumin➢ Autoclaved Water➢ Mineral Oil➢ Formamide➢ Proteinase K➢ High Molecular weight stabilizers such as polyethylene glycol (1% max)
➢ Detergents such as Triton X100, NP40, Tween 20 and SDS/SLS (1% max)
➢ Glycerol (2% max)
➢ DMSO (10% max)
➢ Betaine (1.25-2.5M max)

Doc ID: 482681 rev 0
ADS Biotec and WAVE Optimized are registered trademarks. ©2017 ADS Biotec, Inc. All rights reserved.

www.adsbiotec.com
ADS Biotec Inc. | 7409 Irvington Road | Omaha, NE | USA 68122 | tel: 402- 800-3200
ADS Biotec Limited | 40 Watt Road | Hillington Park | Hillington, Glasgow | UK G52 4RY | +44 (0) 141 8928800
Registered in England and Wales. Registered office: Birketts LLP, 22 Station Road, Cambridge, CB1 2JD Company Registration No. 03257373 VAT Registration No. GB682479101