The 30th International Workshop on
Retroviral Pathogenesis

October 8-12, 2018
Organizers:
Hung Fan, Patrick Green, Kim Hasenkrug,Jiří Hejnar, Masaaki Miyazawa, and Susan Ross
Awaji Yumebutai International Conference Center,Hyogo, Japan

Acknowledgements
The 30th International Workshop on Retroviral Pathogenesis is organized by Hung Fan, University ofCalifornia, Irvine; Patrick Green, The Ohio State University; Kim Hasenkrug, Rocky Mountain
Laboratories, NIH, NIAID; Jiří Hejnar, Institute of Molecular Genetics, Academy of Sciences of theCzech Republic; Masaaki Miyazawa, Kindai University Faculty of Medicine, Japan; and Susan Ross,
University of Illinois College of Medicine, Chicago.
This meeting is officially supported by the National Institutes of Health grant R13CA232205 fromthe National Cancer Institute, NIH, USA; Grant-in-Aid for Scientific Research (KAKENHI) (B)
16H05199 from Japan Society for the Promotion of Science; and Kindai University Faculty ofMedicine. Generous financial supports were provided by Hyogo Prefecture, Nakauchi Tsutomu
Convention Promotion Foundation, Kato Memorial Bioscience Foundation, Tokyo Biochemical
Research Foundation, PLOS Pathogens, and Retrovirology. We are also grateful to the supports anddonations from Shin Nippon Biomedical Laboratories, Ltd.; East Sendai Rheumatic Disease Clinic;
Taiyo Clinic Tokyo; and those from alumni of the Department of Immunology, Kindai University
Faculty of Medicine and the Class of 1982, Tohoku University School of Medicine.

Nakauchi Tsutomu Convention
Promotion Foundation

The 30th International Workshop on Retroviral Pathogenesis
Awaji Yumebutai International Conference Center
October 8-12, 2018
Monday, October 8, 2018
14:0018:00-20:00

Registration (Registration Counter)
Welcome Reception (Reception Hall B)

20:15-21:15

Keynote Lecture 1 (Main Hall) (Introduction by Jiří Hejnar)
A history of the international workshops on retroviral pathogenesis
Hung Fan (University of California, Irvine, CA)

Tuesday, October 9, 2018
7:00-8:30
8:00-8:30

Breakfast (Westin Awaji Hotel)
Speaker presentation uploads (Main Hall)

8:30- 9:30

Keynote Lecture 2 (Main Hall) (Introduction by Hung Fan)
An expanded view of retroviral pathogenesis: Past, present and futurePeter K. Vogt (The Scripps Research Institute, La Jolla, CA)

9:30- 9:40

Coffee Break (Main Hall)

9:40-11:20

Session 1: Entry and Integration (Main Hall)
Chairs: Karen L. Beemon and Marc Sitbon
Isolation of an infectious FeLV subgroup E provirus and discovery of
its viral receptor
Ariko Miyake (Joint Faculty of Veterinary Medicine, Yamaguchi
University, Yamaguchi, Japan)
An automated SLC expression library screen allows the identificationof the elusive receptor for the porcine endogenous retrovirus type B(PERV-B)
Marc Sitbon (Institut de Génétique Moléculaire de Montpellier, CNRS,and Université Montpellier, Montpellier, France)
Avian leukosis virus integration and tumorigenesis
Karen L. Beemon(Department of Biology, Johns Hopkins University, Baltimore, MD)
Studies of MLV infection with an altered integrase C-terminal tail
peptide using the Myc/Runx2 mouse model
Monica Roth (Rutgers Robert Wood Johnson Medical School, Piscataway,NJ)

New functions and updates to the Retrovirus Integration Database(RID) and their applications
Wei Shao (Advanced Biomedical Computing Center, Leidos Biomedical
Research, Inc., Frederick National Laboratory for Cancer Research(FNLCR), Frederick, MD)
11:20-11:35

Coffee Break (Main Hall)

11:35-12:15

Session 2: Epidemiology (Main Hall)
Chair: Christine Kozak
Molecular epidemiology of EIAV IN Europe: Where do the viruses
come from?
Caroline Leroux (Departement of Viral Infections and Comparative
Pathology, UMR754 INRA Lyon 1 University, Lyon, France)
Koala retrovirus, Koalas, and mammalian-retroviral co-evolution
Maribeth V. Eiden (National Institutes of Mental Health, Bethesda, MD)

12:15-13:15
12:45-13:15

Lunch (Reception Hall B)
Speaker presentation uploads (Main Hall)

13:15-14:15

Session 3: Evolution
Chair: Maribeth Eiden
The evolutionary journey of Fv1
Jonathan Stoye (The Francis Crick Institute, London, UK)
Ancient origin and adaptive evolution of the Fv1 retroviral restriction
gene in inbred laboratory mice, the genus Mus and muroid rodents
Christine A. Kozak (Laboratory of Molecular Microbiology, National
Institute of Allergy and Infectious Diseases, Bethesda, MD)
Endogenous feline leukemia virus (FeLV) may provide protection
against exogenous FeLV infection
Elliott S Chiu (Department of Microbiology, Colorado State University,Fort Collins, CO)

14:15-14:30

Coffee Break (Main Hall)

14:30-15:50

Session 4: APOBEC3
Chair: Masamichi Muramatsu
APOBEC-mediated mutagenesis of MMTV in different mouse strains
Jaquelin P. Dudley (The University of Texas at Austin, Austin, TX)

Effect of APOBEC3C on retrovirusesCarsten Münk (Clinic for Gastroenterology, Hepatology, and Infectiology,
Medical Faculty, Heinrich-Heine-University Düsseldorf, Germany)
Mouse APOBEC3 interferes with proteolytic autoprocessing ofGag-Pol polyprotein and inhibits Gag processing
Yoshiyuki Hakata (Kindai University Faculty of Medicine)
Role of APOBEC3 enzymes in the pathology of human papillomavirus
Kousho Wakae (National Institute of Infectious Diseases, Tokyo, Japan)
15:50-18:00

Poster Session (Main Hall)

18:00-19:30
19:00-19:30

Dinner (Reception Hall B)
Speaker presentation uploads (Main Hall)

19:30-21:30

Special Session on Humanized Mice (Main Hall)
Chair and moderator: Yoshio Koyanagi
HIV-1 pathogenesis study using humanized mice
Yoshio Koyanagi (Institute for Frontier Life and Medical Sciences, Kyoto
University, Kyoto, Japan)
Development of novel version of humanized mouse modelTakeshi Takahashi (Central Institute for Experimental Animals, Kawasaki,Japan)
HIV-1 specific immunity in BLT humanized mice
Todd Allen (Ragon Institute of MGH, MIT and Harvard, Boston, MA)
Development of retrovirus-targeting VSV vectors for HTLV-1
treatment in humanized mouse model
Kazu Okuma (National Institute of Infectious Diseases, Tokyo, Japan)

Wednesday, October 10, 2018
7:00-8:30
8:00-8:30

Breakfast (Westin Awaji Hotel)
Speaker presentation uploads (Main Hall)

8:30- 9:30

Keynote Lecture 3 (Main Hall) (Introduction by Masaaki Miyazawa)
Antiretroviral molecular mechanisms of APOBEC3 cytidine
deaminases
Yasumasa Iwatani (National Hospital Organization Nagoya Hospital,Nagoya, Japan)

9:30-10:30

Session 5: Host innate factors (main Hall)
Chair: Masaaki Miyazawa
Using DDX41 knockout mice to study its anti-retroviral and tumor
suppressor activities
Susan R. Ross (University of Illinois at Chicago, College of Medicine,
Chicago, IL)
Dissecting the mucosal HIV-1 interferome
Mario L. Santiago (University of Colorado School of Medicine, Aurora,CO)Transgenic expression of a picornaviral RNA-dependent RNA
polymerase (RdRP) in mice is strongly protective against Friend
retrovirus
Eric M Poeschla (University of Colorado School of Medicine, Aurora, CO)

10:30-11:00

Free discussion (Main Hall)

11:00-18:00

Free discussion and excursions

18:00-20:00

Dinner (Reception Hall B)

20:00-21:00

Keynote Lecture 4 (Main Hall) (Introduction by Susan Ross)
Neutralizing antibody responses to viral infections are linked to thenon-classical MHC class II gene H2-Ob
Tatyana V. Golovkina (University of Chicago, Chicago, IL)Thursday, October 11, 2018
7:00-8:30
8:00-8:30

Breakfast (Westin Awaji Hotel)
Speaker presentation uploads (Main Hall)

8:30- 9:30

Keynote Lecture 5 (Main Hall) (Introduction by Patrick Green)
Host innate and acquired immune responses are the key factors forpathogenesis and therapy in HTLV-1 infection
Mari Kannagi (Graduate School of Medical and Dental Sciences, Tokyo
Medical and Dental University, Tokyo, Japan)

9:30-10:10

Session 6: Adaptive immunity and vaccination (1) (Main Hall)
Chair: Mario Santiago
The requirement of B cell-intrinsic TLR7 for the production ofretrovirus-neutralizing antibodies is overcome by T helper cells in an
epitope-specific manner
Masaaki Miyazawa (Kindai University Faculty of Medicine,Osaka-Sayama, Japan)
Comparison of vector-based vaccines and attenuated retrovirus for
immunization against Friend virus demonstrates induction of strong
protection by distinct mechanisms
Wibke Bayer (Institute for Virology, University Hospital Essen, University
Duisburg-Essen, Essen, Germany)

10:10-10:20

Coffee Break (Main Hall)

10:20-11:15

Session 7: Adaptive immunity and vaccination (2) (Main Hall)
Chair: Judd Hultquist
Protective immune responses elicited by a live attenuated SIV vaccine
required for containment of heterologous SIV infection
Kazuyasu Mori (AIDS Research Center, National Institute of Infectious
Diseases, Tokyo, Japan)
Novel virus-like particle vaccine of bovine leukemia virus efficiently
suppresses disease progression
Yoko Aida (Riken Cluster for Pioneering Research, Wako, Japan)
Intravital imaging of regulatory and effector T cells during an acute
retroviral infection
Lucas Otto (Institute for Virology, University Hospital Essen, Universityof Duisburg-Essen, Essen, Germany)

11:15-11:30

Coffee Break (Main Hall)

11:30-13:25

Session 8: HIV-1-host interactions (Main Hall)
Chairs: Patrick Green and Kei SatoHIV-1-infected T-cell profiling reveals dysregulation of a putative NK
receptor
Michael Schindler (Institute of Medical Virology, University Hospital
Tubingen, Tubingen, Germany)
A functional map of HIV-host interactions in primary human CD4+ Tcells
Judd F. Hultquist (J. David Gladstone Institutes, San Francisco, CA)
Functional (or dysfunctional) conflict of Vif and APOBEC3 inhumanized mice infected with HIV-1
Kei Sato (Institute of Medical Science, The University of Tokyo)
CypA controls the timing of CPSF6 interaction with HIV-1
Vineet N. KewalRamani (National Cancer Institute, Frederick, MD)
Evolution of the HIV-1 RRE during natural infection reveals key
nucleotide changes correlated with altered structure and increased
activity over time.
David Rekosh (University of Virginia, Charlottesville, VA)
Mannose receptor 1 inhibits virus release from HIV-1-infected humanmacrophages
Sayaka Sukegawa (National Hospital Organization Nagoya MedicalCenter, Nagoya, Japan)

13:25-14:25
13:55-14:25

Lunch (Reception Hall B)
Speaker presentation uploads (Main Hall)

14:25-15:25

PLOS Pathogens Writing Workshop
Susan Ross

15:25-15:45

Session 9: HTLV-1 pathogenesis (Main Hall)
Chair: Marie-Louise Hammarskjold
HTLV-1 Tax-1 interacts with SNX27 to regulate cellular localization of
GLUT1
Patrick L. Green (Center for Retrovirus Research, The Ohio State
University, Columbus, OH)

15:45-16:00

Coffee Break (Main Hall)

16:00-17:20

Session 10: Endogenous retroviruses (Main Hall)
Chairs: Jonathan Stoye and Jiří Hejnar

Presence of a shared 5'-leader sequence in ancestral human andmammalian retroviruses and its transduction into feline leukemia
virus
Junna Kawasaki (Joint Faculty of Veterinary Medicine, Yamaguchi
University, Yamaguchi, Japan)
Transcriptional profile of human endogenous retrovirus-K (HML02)
in hepatoblastoma
David F. Grabski (University of Virginia, Charlottesville, VA)
Detection of a functional REC protein in the SK-MEL 28 humanmelanoma cell line
Sara K. Rasmussen (University of Virginia, Charlottesville, VA)
A deletion in the CTE of the mouse NXF1 gene causes behavioral
changes and downregulation of ARC, FOSB, FOS and other synaptic
plasticity genes.
Marie-Louise Hammarskjold (University of Virginia, Charlottesville, VA)
17:20-18:00

Session 11: Pathogenesis and autoimmunity (Main Hall)
Chair: Jaquelin P. Dudley
Mouse mammary tumor virus is necessary to trigger autoimmune
biliary disease in the NOD.C3C4 mouse model of primary biliary
cholangitis
Andrew Mason (University of Alberta, AB, Canada)
Non signal peptide functions of viral signal peptides: MMTV-P14 asparadigm
Jacob Hochman (Alexander Silberman Institute of Life Sciences, The
Hebrew University of Jerusalem, Jerusalem, Israel)

18:00-18:20

Closing remarks and announcements
The Organizing Committee

18:20-19:00

Free discussion

19:00-

Gala dinner (The Banquet Hall, Westin Awaji Hotel)

Friday, October 12, 2018
7:00-8:30

Breakfast (Westin Awaji Hotel)

8:30-Free discussion and departureList of Posters
P1: The molecular epidemiology study of bovine leukemia virus infection in Myanmar
cattleKyaw Kyaw Moe (Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering
Research, Wako, Saitama, Japan)
P2: Endogenous feline leukemia virus (FeLV) may provide protection against exogenous
FeLV infection
Elliott S. Chiu (Department of Microbiology, Colorado State University, Fort Collins, CO)
P3: Stopping an endogenizing retrovirus in its track: Induction of neutralizing antibody
and subsequent reduction in viral load in koalas following vaccination against koala
retrovirus
Olusola Olagoke (Genecology Research Center, Faculty of Science, Health, Education andEngineering, University of the Sunshine Coast, Australia)
P4: Verification of CAT1/SLC7A1 as a receptor candidate of bovine leukemia virus
Lanlan Bai (Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research,Wako, Saitama, Japan)
P5: Fast rate of DNA polymerization during reverse transcription alleviates
APOBEC3-mediated mutagenesis of the prototypic betaretrovirus
Stanislav Indik (Institute of Virology, University of Veterinary Medicine Vienna, Austria)
P6: Bovine leukemia virus (BLV) Gag mutants enhance viral-like particle (VLP) release
Lowela Siarot (Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research,Wako, Saitama, Japan)
P7: Syncytium-forming ability and incorporation of BLV envelope glycoprotein into virion
are regulated by YXXL sequence of transmembrane subunit
Ryosuke Matsuura (Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering
Research, Wako, Saitama, Japan)
P8: Maedi-Visna virus as a model for lentiviral integration and latency
Valgerdur Andrésdóttir (Institute for Experimental Pathology, Keldur, University of Iceland)
P9: PRMT5 is upregulated after BLV infection and selective inhibition enhances viral gene
expression
Wlaa Assi (Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research,Wako, Saitama, Japan)
P10: Mannose receptor 1 inhibits virus release from HIV-1-infected human macrophages
Sayaka Sukegawa (Clinical Research Center, National Hospital Organization Nagoya MedicalCenter, Nagoya,Japan)

P11: Expression of Galectin-9 in normal bone marrow and Friend virus-induced leukemiacells
Tetsuo Tsukamoto (Department of Immunology, Kindai University Faculty of Medicine,Osaka-Sayama, Osaka, Japan)
P12: Intravital imaging of regulatory and effector T cells during an acute retroviral
infection
Lucas Otto (Institute for Virology, University Hospital Essen, University of Duisburg-Essen,Essen,
Germany)
P13: Fate determination with a selected epitope facilitates follicular helper T celldifferentiation and protection against acute retroviral infection
Shigeki Kato (Department of Immunology, Kindai University Faculty of Medicine,Osaka-Sayama, Osaka, Japan)
P14: Control of bovine leukemia virus (BLV) infection using resistant and susceptible
cattleLiushiqi Borjigin (Nano Medical Engineering Laboratory, Riken Cluster for Pioneering
Research, Wako, Saitama, Japan)
P15: Maedi-Visna virus Vif protein modulates autophagy in macrophages
Stefan R. Jónsson (University of Iceland, Institute for Experimental Pathology, Reykjavik,Iceland)
P16: Genetic interplay between ERV-DC14 and Refrex-1
Minh Ha Ngo (Laboratory of Molecular Immunology and Infectious Diseases, The United
Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi, Japan)
P17: Neurodevelopmental tumorigenesis is driven by an endogenous retrovirus
Tara T. Doucet-O'Hare (Section of Infection of the Nervous System, National Institutes of
Neurological
Disorders and Stroke, National Institutes of Health Bethesda, MD)
P18: GFP tagging enables expression of Human T-cell lymphotropic virus-1 p30 protein
but not enough to solubilize it in bacterial expression system
Priyanka Namdev (Department of Biotechnology, Dr. Harisingh Gour University, Sagar, India)
P19: The luminescence syncytium induction assay (LuSIA) is new method for analysing
bovine leukemia virus infectivity
Hirotaka Sato (Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Researchand Virus Infectious Diseases Unit, RIKEN, Saitama, Japan)
P20: Cross-linking mass spectrometry and the characterization of virus-hostprotein-protein interfaces
Robyn M. Kaake (The J David Gladstone Institutes, , San Francisco, CA; San Francisco, CA)