The 30th Hot Spring Harbor International Symposium
Chromatin Potential in Development and Differentiation
The 6th Symposium of the Inter-University Research Network for Trans-Omics Medicine

New technologies meet Biology
January 18 - 19, 2022
Online meeting

Tuesday, January 18, 2022
09:25-09:30

Opening Remarks:
Hiroshi Kimura (Tokyo Institute of Technology)

Session 01:

New Technologies

Chair:

Hiroshi Kimura (Tokyo Institute of Technology)

09:30-10:00

S-01 : Wu Ting (C-ting) (Harvard Medical School, USA)
Doubling down on pairing

10:00-10:30

S-02 : Long Cai (California Institute of Technology, USA)
Spatial multi-omics: RNA and DNA seqFISH+10:30-11:00

S-03 : Yasuyuki Ohkawa (Kyushu University, Japan)
Chromatin structure in skeletal muscle regeneration

11:00-11:05

Short break

11:05-11:25

S-04 : Hiroshi Ochiai (Hiroshima University, Japan)
STREAMING-tag system: A novel technology to analyze the spatiotemporal relationship between transcriptional regulators and transcriptional
dynamics at the single gene level

11:25-11:45

S-05 : Takayuki Nojima (Kyushu University, Japan)
POINTing towards transcription termination

11:45-12:05

Selected from poster abstract
P-12 : Yuki Iwashima (Kanazawa University, Japan)
The role of nuclear pore complex in the transcriptional regulation
P-17 : Hiroaki Kato (Shimane University, Japan)
A chimeric chemical model revealed asymmetric DNA pattern in transcribednucleosomes
Meet the speakers （2 Breakout Rooms）
Chair1: Hiroshi Kimura (Tokyo Institute of Technology)
Chair2: Noriko Saito (The Cancer Institute of JFCR)

12:05-12:20

12:20-13:00

Lunch
1

Session 02:
Chair:

Imaging and Modeling

Yasushi Hiraoka (Osaka University)

13:00-13:30

S-06 : Hiroshi Kimura (Tokyo Institute of Technology, Japan)
Dynamics of histone modification and RNA polymerase II in living cells

13:30-13:50

S-07 : Akatsuki Kimura (National Institute of Genetics, Japan)
Quantification and formulation of the effect of nuclear size on the mobilityof chromatin in the C. elegans embryo

13:50-14:20

S-08 : Tomoko Nishiyama (Nagoya University, Japan)
Single-molecule approaches for understanding genome architecture

14:20-14:40

S-09 : Yuma Ito (Tokyo Institute of Technology, Japan)
Live-cell single-molecule imaging of the dynamic interaction between RNA
polymerase II and chromatin nanostructures

14:40-15:00

S-10 : Shin Fujishiro (Nagoya University, Japan)
The role of dynamic cohesin looping in chromatin compaction and phaseseparation

15:00-15:05

Short break

15:05-15:20

Meet the speakers （2 Breakout Rooms）
Chair1: Yasushi Hiraoka (Osaka University, Japan)
Chair2: Tokuko Haraguchi (Osaka University, Japan)

Poster Session 01
15:20-16:40

Poster Session （odd）

Session 03:

Genome and Protein Structure

Chair:

Jun-ichi Nakayama (National Institute for Basic Biology)

16:40-17:00

S-11 : Rawin Poonperm (RIKEN, Japan)
Establishment of the late-replicating inactive X chromosome during
differentiation

17:00-17:30

S-12 : Ana Pombo (Max Delbrück Center for Molecular Medicine,Germany)
3D genome topologies distinguish pluripotent epiblast and primitive
endoderm cells in the blastocyst

17:30-18:00

S-13 : Patrick Cramer (Max Planck Institute, Germany)
Recent insights into chromatin transcription

18:00-18:30

S-14 : Hitoshi Kurumizaka (The University of Tokyo, Japan)
Structural insights into the dynamics of the chromatin architecture2

18:30-19:00

Meet the speakers （2 Breakout Room）
Chair1: Jun-ichi Nakayama(National Institute for Basic Biology)
Chair2: Hidetoshi Kono (National Institutes for Quantum Science andTechnology)3

Wednesday, January 19, 2022
Session 04:
Chair:Development and Differentiation

Kazuo Yamagata (kindai University)

09:30-10:00S-15 : Hiroyuki Sasaki (Kyushu University, Japan)
Establishment of the proper DNA methylation landscape requires theDNMT3A domains recognizing histone modifications in mouse oocytes

10:00-10:30

S-16 : Wei Xie (Tsinghua University, China)
Epigenetic inheritance and reprogramming during early animal development

10:30-11:00

S-17 : Atsushi Suzuki (Kyushu University, Japan)
Direct reprogramming technology for basic research and clinicalapplications

11:00-11:30

S-18 : Noriko Saitoh (The Cancer Institute of JFCR, Japan)
ELEANOR non-coding RNAs associate with chromatin and regulate the 3D
genome structure in breast cancer

11:30-11:50

Selected from poster abstract
P-06 : Tomoko Sunami (National Institutes for Quantum Science andTechnology, Japan)
FRET analysis of sequence dependence of nucleosomal DNA unwrapping
P-23 : Yuichi Saito (Kyushu University, Japan)
Variant histone H3.3 expression controls the plasma cell differentiation

11:50-12:05

Meet the speakers （2 Breakout Rooms）
Chair1: Kazuo Yamagata (Kindai University)
Chair2: Hiroshi Ochiai (Hiroshima University)

12:05-12:50

Lunch

Poster Session 02
12:50-14:10

Poster Session （even）

Session 05:

Transcription and Mathematical science

Chair:Yoichi Shinkai (RIKEN )14:10-14:40

S-19 : Lacra Bintu (Stanford University, USA)The KRAB domains: high-throughput characterization, single-cell dynamics, and tooldevelopment

14:40-15:10

S-20 : Keiichi Nakayama (Kyushu University, Japan)
Life sciences in the near future: Towards an era of data-driven sciences withartificial intelligence4

15:10-15:30

S-21 : Tetsuya Yamamoto (Hokkaido University, Japan)
Regulation of nuclear bodies by transcription

15:30-15:50

S-22 : Chie Kikutake (Kyushu University, Japan)
Pan-cancer analysis of mutations in open chromatin regions and their
possible association with cancer pathogenesis

15:50-15:55

Short break

15:55-16:10

Meet the speakers（2Breakout Rooms）
Chair1: Yoichi Shinkai (RIKEN)
Chair2: Akatsuki Kimura (National Institute of Genetics)

Session 06:

Chromatin and Epigenetics

Chair:

Ichiro Hiratani (RIKEN)

16:10-16:30

S-23 : Yukako Hattori (Kyoto University, Japan)
Investigating the adaptive role of two histone methyltransferases tocarbohydrate-rich diets in animal growth

16:30-17:00

S-24 : Yoichi Shinkai (RIKEN, Japan)
H3K9 and H3K27 methylation pathways exclusively and coordinately
regulate heterochromatin organization

17:00-17:30

S-25 : Jop Kind (Hubrecht Institute, The Netherlands)
Single-cell multiomic epigenetic profiling in early embryogenesis

17:30-17:45

Meet the speakers （Breakout Room）
Chair: Ichiro Hiratani (RIKEN)

17:45-17:50

Closing Remarks:
Yoshinori Fukui (Director, MIB, Kyushu University)5

Poster Presentation※odd number : Poster Session 01 January 18※even number : Poster Session 02 January 19

P-01 Histone H3K36me2 and H3K36me3 form a chromatin platform essential for DNMT3Adependent DNA methylation in mouse oocytes
Seiichi Yano (Kyushu University)
P-02 Discovery of a novel aminocyclopropenone compound that inhibits BRD4-driven
nucleoporin NUP210 expression and attenuates colorectal cancer growth
Hiroya Kondo (Kanazawa university)
P-03 High Precise Solution Scattering
Masaaki Sugiyama (Kyoto University)
P-04 Unravelling the dynamics and conformational states of multi-domain chromatin binding
protein HP1
Amarjeet Kumar (National Institutes for Quantum and Radiological Science andTechnology (QST))
P-05 Identification of novel regulators of primitive endoderm differentiation through genomescale CRISPR library screeningHitoshi Owada (Hiroshima University)
P-06 FRET analysis of sequence dependence of nucleosomal DNA unwrapping
Tomoko Sunami (National Institutes for Quantum Science and Technology)
P-07 Heterochromatin eraser promotes heterochromatin formation at target gene cluster
Takahiro Asanuma (Hokkaido university)
P-08 Time-lapse tracking of single-cell transcriptomes reveals a role for histone H3 upondormancy breaking in fission yeast
Yuki Shogaki (Waseda University)
P-09 A genome-wide survey of regulatory variants associated with immune system diseases
Naoto Kubota (Kyushu University)
P-10 MyoD Binding in Proliferative Myoblasts during the Cell Cycle
QIANMEI WU (Kyushu University)
P-11 MLL3/MLL4 Histone Methyltranferase Activity Dependent Chromatin Organization atEnhancers during Embryonic Stem Cell Differentiation
Naoki Kubo (Kyushu University)
P-12 The role of nuclear pore complex in the transcriptional regulation
Yuki Iwashima (Kanazawa University)
P-13 Development of CRISPR-Based Rainbow/Barcode Dual Labeling System
Masaki Kawamata (Kyushu University)
P-14 Mechanisms regulating Clr4 histone methyltransferase activity
6

Rinko Nakamura (National Institute for Basic Biology)
P-15 Spatiotemporal Imaging of Histone H2A-DNA Dynamics using HS-AFM
Goro Nishide (Kanazawa University)
P-16 Targeted inhibition of EPAS1-driven IL-31 production by a small-molecule compound
Kazufumi Kunimura (Kyushu University)
P-17 A chimeric chemical model revealed asymmetric DNA pattern in transcribednucleosomes
Hiroaki Kato (Shimane University)
P-18 HDAC inhibitors induce H2A.Z accumulations to gene bodies in specific genes and theirtranscription suppression
Hiroaki Tachiwana (The Cancer Instiute of JFCR)
P-19 Building a Coarse-grained Model of Chromatin for Disentangling the Effects of Histone
Post-Translational Modifications on Nucleosome Packing and Chromatin Structure.
Justin Chan (Institute for Quantum and Life Science (iQLS), QST)
P-20 Discriminative feature of cells characterizes cell populations of interest by a small subsetof genes
Takeru Fujii (Kyushu University)
P-21 Pervasive occurrence of splice-site-creating mutations in genetic disorders
Narumi Sakaguchi (Kyushu University)
P-22 Live imaging of the initiation and elongation forms of RNA polymerase II using
phosphorylation-specific probes
Satoshi Uchino (Tokyo Institute of Technology)
P-23 Variant histone H3.3 expression controls the plasma cell differentiation
Yuichi Saito (Kyushu University)
P-24 Chromatin mobility of X-linked loci and its transcription regulation
Yuko Sato (Tokyo Institute of Technology)
P-25 Epigenomic and genetic approaches to identify Rorc enhancers indispensable for LTi cell
development
Satoshi Kojo (Kyushu University)
P-26 Fission yeast nuclear membrane protein Bqt4 is important for the regulation of nucleolus
movement in the nucleus
Masaru Ueno (Hiroshima University)
P-27 Transcriptomic landscape of whole lung cells after influenza virus infection in mouse
Shin-ichi Koizumi (Kyushu University)
P-28 An analytical system for comprehensive quantitative profiling of bioactive lipids by solid
phase extraction combined with LC/MS/MS
Kohta Nakatani (Kyushu University)
P-29 Cryo-EM structure of the nucleosome containing human parasitic Giardia lamblia
7histones
Shoko Sato (The University of Tokyo)
P-30 Cryo-EM structures of chromatin units from nucleus
Yoshimasa Takizawa (The University of Tokyo)
P-31 Establishment of CTCF looping during medaka embryogenesis
Ryohei Nakamura (The University of Tokyo)
P-32 SmcHD1 underlies the formation of H3K9me3 blocks on the inactive X chromosome inmice
Saya Ichihara (Kindai University)
P-33 Mechanisms of mismatch-induced nucleosome remodeling during eukaryotic DNA
mismatch repair
Eiichiro Kanatsu (Kyushu University)8