FOR RESEARCHUSE ONLY
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INSTRUCTIONS FOR PRODUCTGlySERIAS, 2000 units (A0-GS1-020)
Digestion of up to 2 mg fusion protein

Last revised Jun 2022

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WORKFLOW
Quick Guide

1Prepare GlySERIAS™
• Reconstitute GlySERIAS in 50 µl ddH2O
to a concentration of 40 units/µl.

2

Add GlySERIAS™
• Add 1 unit GlySERIAS /1 µg fusion protein.

3Digestion• Incubate for 1 h at 37°C.

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PRODUCT DESCRIPTIONGlySERIAS is an enzyme that digests flexible
linkers in engineered fusion proteins containing
two or more proteins or peptides. It is active on(Gly4Ser) n, Gly xSer y and polyglycine linkers.
The repetitive design of the linker will lead toseveral simultaneous digestion sites andseparation of the previously linked components.
Optimal activity occurs at 37°C, pH 7.6 undernative conditions.
GlySERIAS is cloned from phage K and is recombinantly expressed in E. coli. The enzyme contains
a His-tag and has a molecular weight of 18 kDa.

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Unit Definition
One unit GlySERIAS digests ≥ 95% of 1 μg
dulaglutide at a minimum of one site, whenincubated in TBS (50 mM Tris-HCl, 150 mMNaCl, pH 7.6) at 37°C for 15 minutes.

Content and Storage
GlySERIAS is supplied lyophilized in 50 mM Tris-HCl,
150 mM NaCl, pH 7.6, with no preservatives added.
Upon arrival, the enzyme should be stored at -20°C.
After reconstitution, the GlySERIAS enzyme isstable for at least 1 month at +4-8°C.
GlySERIAS is for R&D use only.

5DETAILED PROTOCOL
Additional Materials Required• Digestion buffer: TBS, pH 7.6.
Sample Preparation
Prepare the fusion protein in the digestion buffer1.
The final protein concentration in the digestion
reaction should be 1-5 mg/ml.
1 Prepare GlySERIAS™

• Reconstitute GlySERIAS in
50 μl ddH2O to 40 units/μl.
2 Add GlySERIAS™

• Add 1 unit GlySERIAS / 1 µg fusion protein2.
3 Digestion• Incubate for 1 h at 37°C3,4.
Notes
1. Optimal activity is achieved in TBS buffer pH 7.6. The enzyme isactive in pH 6.5-9 but the digestion efficiency may differ between
different GS-linked proteins.
2. A higher enzyme concentration may increase digestion efficiency ofindividual GS-linked proteins. This requires optimization.
3. A shorter incubation time will allow for a more complete coverageof linker sequence whereas a longer incubation time will reducecomplexity and result in more homogeneous subunits.
4. The linker may not be completely removed from the GS-linked proteins.

6Quality Control
GlySERIAS is tested to meet thespecifications and lot-to-lot consistency.
GlySERIAS is tested for absence of
microbial contamination with blood agar
plates, Sabouraud dextrose agar plates andfluid thioglycollate medium.

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patents, trademarks and copyrights owned or controlled by Genovis AB.
For more information about commercial rights, please contact the Genovis
team at licensing@genovis.com.
Genovis products are intended for research use only. They are not intended
to be used for therapeutic or diagnostic purposes in humans or animals.
All goods and services are sold subject to Genovis’ General Terms andConditions of Sale.
© Genovis AB.

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