MP Biomedicals, LLC

29525 Fountain Parkway
Solon, Ohio 44139

Telephone: 440/337-1200
Toll Free: 800/854-0530
Fax: 440/337-1180
mailto: biotech@mpbio.com
web: http://www.mpbio.com

TECHNICAL INFORMATION
Catalog Number: 1020120, 1020122
Medium 199 with Earle's Salt (E199), powder, with L-glutamine, without sodium bicarbonate
Description: Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source
for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad speciesapplicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro
cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.
References:– Morgan, J.F., et. al., Proc. Soc. Exptl. Biol. Med., v. 51, 1 (1950).
– Morgan, J.F., et. al., J. Natl. Cancer Inst., v. 16, 557 (1955).
– Parker, R.C., In: Methods of Tissue Culture, 3rd Ed., Harper & Row, New York (1961).
Note: The DL-amino acids of the original formulation have been replaced by L-amino acids at half the concentration.
Formulation:
Components
Amino Acids
L-Alanine
L-Arginine HCl
L-Aspartic Acid
L-Cysteine HCl H2O
L-Cystine 2HCl
L-Glutamic Acid
L-Glutamine
Glycine
L-Histidine HCl H2O
L-Hydroxyproline
L-Isoleucine
L-Leucine
L-Lysine HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine

Mol. Wt.

Mol. (mM)

89.1

0.28

174.2

0.40

133.1

0.23

175.6

0.0006

313.2

0.08

147.1

0.51

146.1

0.68

75.07

0.67

209.6

0.10

131.1

0.08

131.2

0.15

131.2

0.46

182.6

0.38

149.2

0.10

165.2

0.15

115.1

0.35

mg/L

25.00070.00030.0000.110
26.00075.000100.000
50.00021.88010.00020.00060.00070.00015.00025.00040.00025.000

105.1

0.24

L-Threonine
L-Tryptophan
L-Tyrosine 2Na 2H2O
L-Valine
Vitamins

Choline Chloride
D-Calcium Pantothenate
DL-a-Tocopherol PO4 Na2
Folic Acid
L-Ascorbic Acid
Menadione Sodium Bisulfite 3H2O
myo-Inositol
NiacinNicotinamide
para-Aminobenzoic Acid
Pyridoxine HCl
Riboflavin
Thiamine HCl
Vitamin A Acetate

0.25

204.2

0.05

261.2

0.22

117.1

0.21

244.3

0.00004396.7

0.00025139.6

0.00358238.3

0.00004554.7

0.00002441.4

0.00002176.1

0.00028330.2

0.00006180.2

0.00028123.1

0.00020122.130.00020137.1

0.00036205.6

0.00024376.4

0.00003337.3

0.00003328.5

0.00043

111

1.80

404

0.0018

120.4

0.81

74.55

5.37

58.44

116.36159.96

0.88

134.1

0.0037347.2

0.0006386.4

0.0259605.2

0.001710.00057.87025.000

BiotinCalciferol

119.1
30.000

0.010
0.100
0.500
0.010
0.010
0.010
0.050
0.019
0.050
0.025
0.025
0.050
0.050
0.010
0.010
0.140

Inorganic Salts
Calcium Chloride [CaCl2]
Ferric Nitrate [Fe(NO3)3 9H2O] Nonahydrate
Magnesium Sulfate [MgSO4]
Potassium Chloride [KCl]Sodium Chloride [NaCl]
Sodium Phosphate Monobasic [NaH2PO4 H2O] Monohydrate
Other

200.000
0.720
97.700400.000
6800.000
140.000

2-Deoxy-D-Ribose
5-Adenylic Acid H2O
Adenine Sulfate
ATP 2Na 3H2O

0.500
0.200
10.0001.000

Cholesterol
Dextrose
D-Ribose
Glutathione (reduced)
Guanine HCl
Hypoxanthine Na
Phenol Red Sodium Salt
Sodium Acetate
ThymineTween 80
UracilXanthine Na

386.7

0.0005180.2

5.5494150.1

0.0033307.330.0002187.550.0016159.080.0022376.4

0.026682.03

0.6095126.1

0.0024n/an/a112.1

0.0027174.1

0.00200.200
1000.000
0.500
0.050
0.300
0.354
10.00050.000
0.300
5.000
0.300
0.340

Add
NaHCO3 Powder (g/L)
NaHCO3 7.5% Solution (mL/L)

2.200
29.400Availability:
1X Liquid:
Medium 199 with Earle's Salts:
Catalog Number
Description
1220354
1X E199 with L-glutamine
1220249
1X E199 without L-glutamine
12202541220449
1X E199 with 20 mM HEPES, without sodium bicarbonate, L-glutamine

Size
500 ml100 ml500 ml100 mlMedium 199 with Hanks' Salts:
Catalog Number
Description
1223354
1X H199 with L-glutamine
1223249
1X H199 without L-glutamine
1223254Size
500 ml100 ml500 ml10X Liquid:
Medium 199 with Earle's Salts:
Catalog Number
Description
1420049
10X E199 without sodium bicarbonate, L-glutamine
1420054Size
100 ml500 mlMedium 199 with Hanks' Salts:
Catalog Number
Description
1423054
10X H199 without sodium bicarbonate, L-glutamine

Size
500 mlPowders:Medium 199 with Earle's Salts:
Catalog Number
Description
1020120
E199 with L-glutamine, without sodium bicarbonate
1020122Size
10 x 1 liter1 x 10 literMedium 199 with Hanks' Salts:
Catalog Number
Description
1023120
H199 with L-glutamine, without sodium bicarbonate
10231221023124Size
10 x 1 liter1 x 10 liter
1 x 50 liter