േ‛ታ㛎ߩ⥄ਥ▤ℂߩផㅴߦะߌߡ
㧙HS ⽷࿅ߩേ‛ታ㛎ᄖㇱ⹏ଔ࡮ᬌ⸽೙ᐲ㧙౏⋉⽷࿅ᴺੱࡅࡘ࡯ࡑࡦࠨࠗࠛࡦࠬᝄ⥝⽷࿅േ‛ታ㛎ታᣉᣉ⸳⹺⸽࠮ࡦ࠲࡯
૒‫ᧁޘ‬ᒎ↢ߪߓ߼ߦ
ᐔᚑ 18 ᐕߩෘഭ⋭ᜰ㊎ߩ╷ቯᓟ‫ޔ‬ᜰ㊎ࠍ⊒಴ߒߚ 3 ⋭ᐡߩਛߢ‫ޔ‬േ‛ታ㛎ታᣉᣉ⸳ߩᄖㇱ⹏ଔ૕೙߇ᢛ஻ߐࠇߡ޿ߥ߆ߞߚෘഭ⋭ᚲ▤ᬺ⒳ߩᄖㇱ⹏ଔᯏ㑐ߩᔅⷐᕈ߇㑐ଥ⠪߆ࠄⷐᦸߐࠇ‫ޔ‬ᑷ⽷࿅ߪ㑐ଥᬺ⇇ߩ⁁ᴫ╬ࠍ〯߹߃⚂ 1 ᐕ㑆ߩḰ஻ᦼ㑆ࠍ⚻ߡ‫ޔ‬ᐔᚑ
20 ᐕߦᄖㇱ⹏ଔ੐ᬺࠍഃ⸳ߒߚ‫ޕ‬
ᐔᚑ 24 ᐕߦേ‛ߩᗲ⼔෸߮▤ℂߦ㑐ߔࠆᴺᓞ㧔એਅ‫ޔ‬㨬േᗲᴺ㨭ߣ޿߁‫ޕ‬㧕ߩ৻ㇱᡷᱜ
߇޽ࠅ‫⥄ޔ‬ਥ▤ℂߩᬌ⸽ߣߒߡ‫ޔ‬ᄖㇱ⹏ଔࠍᵴ↪ߔࠆߎߣߦߟ޿ߡℂ⸃߇ᷓ߹ߞߚߣ⠨߃ࠄࠇࠆ‫ޕ‬േᗲᴺߩᡷᱜߩ⚻✲ߣߣ߽ߦ੐ᬺ㐿ᆎ 5 ᐕ⋡ࠍㄫ߃ߚ੐ᬺߦߟ޿ߡ‫੺⚫ߏޔ‬ߔࠆߎߣߣߒߚ޿‫ޕ‬㧝㧚ᐔᚑ 24 ᐕേ‛ᗲ⼔▤ℂᴺᡷᱜߣᣉⴕౝኈ
㧝㧕ᡷᱜᴺ᩺ߩክ⼏⚻ㆊ
ᐔᚑ 17 ᐕߩേᗲᴺߩ৻ㇱᡷᱜᤨߩⷙቯߦࠃࠅ‫ޔ‬ᣉⴕᓟ 5 ᐕᓟࠍ⋡ㅜߦ⷗⋥ߒࠍⴕ߁ߎߣߣߐࠇߚߎߣߦ઻޿‫ޔ‬ᐔᚑ 22 ᐕ 8 ᦬ਛᄩⅣႺክ⼏ળേ‛ᗲ⼔ㇱળߦ⸳⟎ߐࠇߚዊᆔຬળߢᬌ⸛߇㐿ᆎߐࠇߚ‫ޕ‬หክ⼏ળߢߪ 25 ࿁ߦࠊߚࠆ⼏⺰߇ߥߐࠇ‫ޔ‬ᐔᚑ 23 ᐕ 12᦬ߩࡄࡉ࡝࠶ࠢࠦࡔࡦ࠻ࠍ⚻ߡ‫ޔ‬ታ㛎േ‛ߦ㑐ߔࠆ⼏⺰ߪ‫⊓ޔ‬㍳೙ߩዉ౉ߩนุߪਔ⺰૬⸥ߩᒻߢႎ๔ᦠ߇ߣࠅ߹ߣ߼ࠄࠇߚ‫ޕ‬ᴺᓞᡷᱜߦ߻ߌߡ‫ޔ‬ᐔᚑ 24 ᐕ 8 ᦬ߦⴐ⼏㒮⺞ᩏዪⅣႺ⺞ᩏቶ߇‫ޟ‬േ‛ߩᗲ⼔෸߮▤ℂࠍ߼ߋࠆ⃻⁁ߣ⺖㗴‫ޠ‬1)ߣߒߡ‫ࠍ⚦⹦ߩ╬⁁⃻ޔ‬ขࠅ߹ߣ߼ߡ޿ࠆ‫ޕ‬ߘߩᓟߪ⼏ຬ┙ᴺߣߒߡ৻ㇱᡷᱜ߇ᬌ⸛ߐࠇ‫ޔ‬ᐔᚑ 24 ᐕ 8 ᦬ 28 ᣣߦⴐ⼏㒮ⅣႺᆔຬ
ળߦ߅޿ߡᴺᓞ᩺ߩ⿠⨲࡮ክ⼏ 2㧕‫ޔ‬หᣣߩෳ⼏㒮ߩⅣႺᆔຬળߩክ⼏ࠍ⚻ߡ⠉ 29 ᣣߦෳ⼏㒮ߢน᳿ᚑ┙ߒ‫ޔ‬9 ᦬ 5 ᣣߦ౏Ꮣߐࠇߚ‫੹ޕ‬࿁ߩᡷᱜߢߪ‫ޔ‬ታ㛎േ‛ߦ㑐ߒߡߩᴺᓞߩᡷᱜ੐㗄ߪߥ޿߇‫⼏ⴐޔ‬㒮ⅣႺᆔຬળ᳿⼏෸߮ෳ⼏㒮ⅣႺᆔຬળ㒝Ꮺ᳿⼏ 3㧕ߣߒߡ‫ޟ‬᡽ᐭߪേ‛ߩᗲ⼔෸߮▤ℂߩㅺ⿛ߩផㅴ߇ੱߣേ‛ߩ౒↢ߔࠆ␠ળߩታ⃻ߦਇนᰳߢ
޽ࠆߎߣߦ㐓ߺ‫ᧄޔ‬ᴺࠍᣉⴕߔࠆߦᒰߚߞߡߪ‫ޔ‬ᰴߩ੐㗄ߦ⇐ᗧߒ‫ߩߘޔ‬ㆇ↪ߦߟ޿ߡਁోࠍ߈ߔߴ߈ߢ޽ࠆ‫ޕ‬
㧔৻ㇱ⇛㧕
৾‫ޔ‬
ታ㛎േ‛ߩขᛒ޿ߦଥࠆᴺ೙ᐲߩᬌ⸛ߦ㑐ߒߡߪ‫ޔ‬㑐ଥ⠪ߦࠃࠆ⥄ਥ▤ℂߩข⚵෸߮㑐ଥᐭ⋭ߦࠃࠆታᘒᛠីߩข⚵ࠍ〯߹߃ߟߟ‫ޔ‬࿖㓙⊛ߥⷙ೙ߩേะ߿⑼ቇ⊛⍮⷗ߦ㑐ߔࠆᖱႎߩ෼㓸ߦദ߼ࠆߎߣ‫ޔߚ߹ޕ‬㑐ଥᐭ⋭ߣߩㅪ៤ࠍߪ߆ࠅߟߟ‫ޔ‬㧟R㧔ઍᦧᴺߩㆬᛯ‫↪૶ޔ‬ᢙߩ೥ᷫ‫ߩ∩⧰ޔ‬シᷫ㧕ߩታലᕈߩᒝൻ╬ߦࠃࠅ‫ޔ‬ታ㛎േ‛ߩ⑔␩ߩታ⃻ߦദ߼ࠆߎߣ‫ޕ‬‫ޔࠇߐߣޠ‬േ‛ታ㛎ߩታᣉߦ޽ߚࠅ㧟R ߦ㈩ᘦߒߚታᣉߩᒝൻ߇᳞߼ࠄࠇࠆߎߣߦߥߞߚ‫ޕ‬࿖ળߢߩክ⼏ߢߪ‫ޔ‬ታ㛎േ‛ߦߟ޿ߡ‫ޔ‬ᐔᚑ 24 ᐕ 8 ᦬ 28 ᣣߦ㐿௅ߐࠇߚⴐ⼏㒮ⅣႺ
ᆔຬળߢឭ᩺⠪ߩ↰ፉᆔຬߪ‫౓ޔ‬ᐶ⋵ߩ᧦଀ਗߦዯߌ಴೙ߩታᣉ‫ޔ‬㧟R ߩ⟵ോൻࠍᬌ⸛
ߒߚ߇‫ޔ‬
ᣉ⸳ᖱႎߩ㐿␜ߦࠃࠆ៊ኂ߿↢๮⑼ቇ⎇ⓥ⊒ዷߩ㓚ኂ߆ࠄ⷗ㅍࠅߣߥߞߚߎߣ‫ޔ‬⥄ਥ⊛ߥദജࠍ࿑ࠆ৻ᣇߢਇᢿߩᬌ⸛ࠍⴕ߁ߴ߈ߣ⊒⸒ߒߡ޿ࠆ‫ޕ‬ෳ⼏㒮ⅣႺᆔຬળߦ
߅޿ߡ߽ห᭽ߩ⺑᣿߇ߥߐࠇߚ‫ⴐޔߚ߹ޕ‬ෳਔ㒮ߣ߽ᡷᱜ᩺ߩน᳿ߣߣ߽ߦ‫ޔ‬หᢥߩᆔຬળ᳿⼏෸߮㒝Ꮺ᳿⼏ߦߟ޿ߡోળ৻⥌ߢ᳿⼏ߐࠇߚ‫ޕ‬
㧞㧕ᡷᱜᴺ᩺ߩᣉⴕ ᡷᱜേᗲᴺߪᐔᚑ 25 ᐕ 9 ᦬ 1 ᣣߦᣉⴕߐࠇ‫ޔ‬േ‛ߩᗲ⼔෸߮▤ℂߦ㑐ߔࠆᣉ╷ࠍ✚ว⊛ߦផㅴߔࠆߚ߼ߩၮᧄ⊛ߥᜰ㊎ߩᡷᱜ‫ޔ‬ታ㛎േ‛㑐ଥߢߪ‫ޔ‬ታ㛎േ‛ߩ㘺㙃෸߮଻▤ਗ߮ߦ⧰∩ߩシᷫߦ㑐ߔࠆၮḰ߇ᡷᱜߐࠇߚ‫ޕ‬േ‛ߩᗲ⼔෸߮▤ℂߦ㑐ߔࠆᣉ╷ࠍ✚ว⊛ߦផㅴߔࠆߚ߼ߩၮᧄ⊛ߥᜰ㊎ 4)ߢߪ‫ޔ‬ታ㛎േ‛ߦߟ޿ߡߪᴺᡷᱜߩⴐෳਔ㒮ߩክ⼏ߩ㓙ߩઃᏪ᳿⼏ߩౝኈ߇⋓ࠅㄟ߹ࠇߡ޿ࠆ‫ޕ‬ ߹ߚ‫ޔ‬ታ㛎േ‛ߩ㘺㙃෸߮଻▤ਗ߮ߦ⧰∩ߩシᷫߦ㑐ߔࠆၮḰߦ߅޿ߡߪ‫⥸৻ޔ‬ේೣߦቯᦼ⊛ߦᒰ⹥ၮḰ╬ߩㆩ቞⁁ᴫߦߟ޿ߡὐᬌࠍⴕ޿‫ޔ‬
ㆡಾߥᣇᴺߦࠃࠅ౏⴫ߔࠆߎߣ‫ޔ‬ᒰ⹥ὐᬌ⚿ᨐߦߟ޿ߡߪน⢻ߥ㒢ࠅ‫ޔ‬ᄖㇱߩᯏ㑐╬ߦࠃࠆᬌ⸽ࠍⴕ߁ࠃ߁ദ߼ࠆߎߣ߇
ⷙቯߐࠇࠆߣߣ߽ߦ‫ޔ‬㘺㙃଻▤ߩᣇᴺߩ㗄ߦᔅⷐߥஜᐽߩ▤ℂਗ߮ߦߘߩേ‛ߩ⒳㘃‫ޔ‬ୃᱜ╬ࠍ⠨ᘦߒߚ㘺㙃଻▤ߩߚ߼ߩⅣႺ⏕଻߇ㅊടߐࠇߡ޿ࠆ‫ޕ‬㧞㧚⹺⸽੐ᬺߩ᭎ᴫ
㧝㧕੐ᬺ㐿ᆎߩ⚻✲ߣ⹺ቯታ❣
 ೨࿁ᐔᚑ 17 ᐕߩേᗲᴺߩᡷᱜߦၮߠ߈‫ޔ‬ෘ↢ഭ௛⋭ߩᚲ▤ߔࠆታᣉᯏ㑐ߦ߅ߌࠆേ
‛ታ㛎╬ߩታᣉߦ㑐ߔࠆၮᧄᜰ㊎㧔એਅ‫ޔ‬㨬ၮᧄᜰ㊎㨭ߣ޿߁‫ޕ‬㧕߇╷ቯߐࠇ‫ޔ‬ᐔᚑ 18 ᐕ

6 ᦬ 1 ᣣߦᣉⴕߐࠇߚ‫ޔߚ߹ޕ‬ᣣᧄቇⴚળ⼏ߩࠟࠗ࠼࡜ࠗࡦ߽␜ߐࠇߚ‫ࠍࠄࠇߎޕ‬ฃߌ‫ޔ‬
⽷࿅ߢߪ‫ޔ‬ᐔᚑ 19 ᐕ 11 ᦬߆ࠄḰ஻ᆔຬળࠍ⊒⿷ߐߖ‫ߩߘޔ‬ᬌ⸛⚿ᨐࠍ߰߹߃‫ޔ‬ᐔᚑ 20ᐕ 7 ᦬ᧃߦ⹺⸽੐ᬺࠍ㐿ᆎߒߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬ቇⴚળ⼏ߩࠟࠗ࠼࡜ࠗࡦߢߪ‫ޟ‬ᒰ⹥ᯏ㑐╬એᄖߩ⠪ߦࠃࠆᬌ⸽ࠍⴕ߁ߎߣࠍ⠨ᘦߔࠆ‫ޔ߇ࠆ޿ߡࠇߐߣޠ‬ၮᧄᜰ㊎ߦ߅޿ߡᄖ
ㇱᬌ⸽ߦߟ޿ߡߪ⸅ࠇࠄࠇߡ޿ߥ޿ߎߣ߆ࠄ‫ޔ‬ೋᐕᐲߪ 4 ઙ(4 ᯏ㑐㧕‫ޔ‬2㧝ᐕᐲߦ߅޿ߡ
߽ 5 ઙ㧔4 ᯏ㑐㧕ߦߣߤ߹ߞߚ‫ޕ‬22 ᐕᐲߦ߅޿ߡ‫⽷ޔ‬࿅ߩਥⷐߥ੐ᬺߢ޽ࠆ᡽╷ഃ⮎✚ว⎇ⓥ੐ᬺ╬ߦ㑐ߒߡෘ↢ഭ௛⋭ߩ⋭ౝ઀ಽߌ߇ታᣉߐࠇ‫⽷ޔ‬࿅ߩേะ߇ਇ᣿⏕ߥ⁁ᴫߣߥߞߚߎߣ߽޽ࠅ‫ޔ‬4 ઙ(2 ᯏ㑐㧕ߣ⹺⸽ᢙߪૐㅅߒߚ‫ޕ‬ ᐔᚑ 23 ᐕ 9 ᦬ߩቇⴚળ⼏ಽ⑼ળߦ߅޿ߡ‫ޔ‬ᣣᧄ⵾⮎Ꮏᬺળࠃࠅᄖㇱ⹏ଔߩታᣉߦࠃ
ࠆ⥄ਥ▤ℂߩలታߥߤߩข⚵ߺߩႎ๔߇ߥߐࠇߚߎߣߥߤࠍฃߌ‫ޔ‬23 ᐕᐲ߆ࠄ↳⺧߇Ⴧടߒ‫ޔ‬ᒰ⹥ᐕᐲߩᣂⷙ⹺ቯᯏ㑐ߪ 7 ઙ㧔2 ࿁⋡ߩ⹺ቯᯏ㑐ࠍวࠊߖ 11 ઙ㧕ߣߥߞߚ‫ޕ‬24ᐕᐲߪᣂⷙ⹺ቯᯏ㑐ߪ 38 ઙ㧔2 ࿁⋡ߩ⹺ቯᯏ㑐ࠍวࠊߖ 43 ઙ㧕ߣᄢ᏷ߥჇߣߥߞߡ޿
ࠆ‫ޕ‬25 ᐕᐲߦߟ޿ߡߪ 10 ᦬ᧃ⃻࿷ߢᣂⷙ⹺⸽ᯏ㑐ߪ 17 ઙߢ޽ࠅ‫ޔ‬24 ᐕᐲࠃࠅዋߥ޿
߽ߩߩ‫ޔ‬23 ᐕᐲࠍ⿥߃ࠆᣂⷙ⹺ቯᢙߣߥࠆ‫ޕ‬25 ᐕ 10 ᦬ᧃ⃻࿷ߩ⹺ቯᯏ㑐ᢙߪ 75 ᯏ㑐ߢ޽ࠆ‫ޕ‬
ߥ߅‫ޔ‬22 ᐕᐲߦ߅޿ߡ‫ޔ‬㑐ଥ࿅૕ࠃࠅ⽷࿅ߩᄖㇱ⹏ଔߦ㑐ߒߡ‫⹏ޔ‬ଔߦะߌߡߩḰ஻Ბ㓏ߢ⋧⺣ࠍฃߌߡ߶ߒ޿ߣߩⷐᦸ߇޽ࠅ‫⺣⋧ޔ‬೙ᐲࠍዉ౉ߒߚ‫ౕޕ‬૕⊛ߦߪ‫⹏⸽⹺ޔ‬ଔຬߣߩ㕙⺣‫ޔ‬ᦠ㕙෶ߪታ࿾⺞ᩏߦࠃࠅၮᧄᜰ㊎߳ߩㆡวᕈߦ㑐ߔࠆ⋧⺣߇น⢻ߣߥࠅ‫ޔ‬23 ᐕᐲߦߥࠅ‫ޔ‬1 ઙߩ೑↪߇޽ߞߚ‫ޕ‬㧞㧕⺞ᩏߩᵹࠇߣ⺞ᩏᣇᴺ੐೨ߦឭ಴޿ߚߛ޿ߚᦠ㕙ࠍ߽ߣߦ‫ޔ‬ታ࿾⺞ᩏߢߪ‫ޔ‬ᣉ⸳ߩⷙᮨ㧔㘺㙃଻▤ᣉ⸳෸߮േ‛ታ㛎ቶ╬ߩ㕙Ⓧ㧕ߦวࠊߖߡ‫ޔ‬1 ᣣ㨪2 ᣣ㑆ࠍ߆ߌ‫ޔ‬ᣉ⸳ߩ᭎ⷐ⺑᣿‫ޔ‬ᯏ㑐ౝⷙ⒟࡮
ᚻ㗅ᦠ╬‫ޔ‬േ‛ታ㛎ᆔຬળߩክᩏ╬ߦ㑐ߔࠆ⾗ᢱ‫ޔ‬㘺⢒࡮ᣉ⸳ߩ▤ℂߦ㑐ߔࠆ⾗ᢱ‫ޔ‬ᢎ⢒⸠✵‫⥄ޔ‬Ꮖὐᬌ‫ޔ‬ᖱႎ౏㐿ߦ㑐ߔࠆ⾗ᢱߩ⏕⹺‫ޔ࡯ࠕ࠷ࡏ࡜ޔ‬ᯏ㑐ߩ㐳෸߮േ‛ታ㛎ᆔຬળᆔຬ㐳ߣߩ㕙⺣ࠍⴕߞߡ޿ࠆ‫ࠍ⸽⹺ޔߪߡ޿ߟߦ࡯ࠕ࠷ࡏ࡜ޔߚ߹ޕ‬ฃߌࠆᣉ⸳
ߩฦേ‛⒳ߏߣߦ㘺㙃଻▤⁁ᴫ߇ᛠីߢ߈ࠆߎߣࠍ⋡ᮡߣߒߡታᣉߒߡ߅ࠅ‫ޔ‬น⢻ߥ㒢ࠅታᣉਛߩ⹜㛎࡮ታ㛎ߩ⁁ᴫ‫ޔ‬㘺⢒૞ᬺ߇ᛠីߢ߈ࠆࠃ߁දജࠍ߅㗿޿ߒߡ޿ࠆ‫ޕ‬ ታ࿾⺞ᩏߩᦨᓟߦ‫⹏⸽⹺ޔ‬ଔຬߦࠃࠆᥳቯ⊛ߥ⹏ଔࠍญ㗡ߢવ㆐ߒߡ޿ࠆ߇‫ޔ‬ᱜᑼߥ⹏ଔ⚿ᨐߦߟ޿ߡߪ‫⹏ޔ‬ଔᆔຬળߦ߅ߌࠆ⹏ଔᓟ‫ޔ‬੐ോዪࠃࠅᢥᦠ㧔⹏ଔ⚿ᨐႎ๔ᦠ㧕ߦࠃࠅવ㆐ߒߡ޿ࠆ‫ޕ‬㧟㧕⹏ଔߩⷞὐ
⹺ቯߦ߅ߌࠆ⹏ଔၮḰߪၮᧄᜰ㊎߳ߩㆡวᕈߣߒߡታᣉߒߡ߅ࠅ‫ޔ‬ၮᧄᜰ㊎ߦ߅޿ߡᒁ↪ߐࠇߡ޿ࠆേᗲᴺ‫ޔ‬㘺㙃଻▤ၮḰ‫࠽ࡋ࠲࡞ࠞޔ‬ᴺ╬േ‛ታ㛎ߦ㑐ㅪߔࠆ⻉ᴺ઎߽⠨ᘦߒߡታᣉߒߡ޿ࠆ‫ޕ‬⹏ଔߩࡐࠗࡦ࠻ߣߒߡᰴߩ 4 ὐࠍ޽ߍߡ߅߈ߚ޿‫ޕ‬Ԙታᣉᯏ㑐ߦ߅޿ߡ⥄ਥ▤ℂ૕೙߇ᢛ஻ߐࠇߡ޿ࠆߎߣ
ԙേ‛ታ㛎ᆔຬળ߇ߘߩᯏ⢻ࠍ⊒ើߒߡ޿ࠆߎߣ
Ԛ⥄Ꮖὐᬌ߇ታᣉߐࠇ‫ޔ‬ታᣉ⚿ᨐߩࡈࠜࡠ࡯ࠕ࠶ࡊ߇ߥߐࠇߡ޿ࠆߎߣ
ԛᢎ⢒⸠✵߇⛮⛯⊛ߦታᣉߐࠇߡ޿ࠆߎߣ
․ߦേ‛ታ㛎ᆔຬળ߇ᯏ⢻ࠍ⊒ើߔࠆߚ߼ߦߪ‫ޔ‬ክᩏ⾗ᢱߦ߅޿ߡઍᦧᴺߩᬌ⸛‫ޔ‬േ‛ᢙߩᩮ᜚‫ߩ∩⧰ޔ‬シᷫߦ㑐ߒߡ⹏ଔน⢻ߥౝኈ߇✂⟜ߐࠇߡ޿ࠆߎߣ‫ߩߘޔߟ߆ޔ‬ౝኈߦኻߒߡ‫⑼ޔ‬ቇ⊛ⷰὐ‫ޔ‬േ‛⑔␩ߦ㑐ߔࠆ࿖㓙⊛ߥ⁁ᴫ߽〯߹߃ߚⷰὐ߆ࠄክᩏ߇ታ
ᣉߐࠇߡ޿ࠆߎߣࠍ‫ޔ‬ㅘ᣿ᕈ࡮⺑᣿⽿છߦߟ޿ߡ߽㈩ᘦߒߡឭ␜ߢ߈ࠆߎߣ߇᳞߼ࠄࠇ
ࠆ‫⺞ޕ‬ᩏᤨߦߪ‫ޔ‬น⢻ߥ㒢ࠅേ‛ታ㛎ᆔຬળߦ߅ߌࠆክᩏ⁁ᴫ෸߮ታ㛎ߩታᣉ⁁ᴫ╬ࠍឭ␜ߐࠇߚ⾗ᢱߦᓥߞߡᛠីߒ‫ޔ‬㧟R ߩේೣߦᓥߞߚേ‛ታ㛎ታᣉߩขࠅ⚵ߺߩะ਄ߦ
⾗ߔࠆࠃ߁േ‛⑔␩ߩⷰὐ߆ࠄࠦࡔࡦ࠻ߒߡ޿ࠆ‫ޕ‬ ߹ߚ‫⥄ޔ‬Ꮖὐᬌߦߟ޿ߡ߽‫ޔ‬ၮᧄᜰ㊎ߦቯ߼ࠄࠇߚ▸࿐ߦߟ޿ߡታᣉߒ‫ޔ‬ᔅⷐߥᡷༀ
߇ߥߐࠇߡ޿ࠆߎߣ‫ޔ‬ᢎ⢒⸠✵ߦߟ޿ߡߪേ‛୶ℂ㕙ߩᢎ⢒ߦวࠊߖߡ㤗㈮ᴺ‫ޔ‬቟ᭉᱫ
ᴺ╬ߩታ㛎ᚻᛛ߽฽߼ߡⴕࠊࠇࠆߎߣ߇ᦸ߹ߒ޿ߣ⠨߃ߡ޿ࠆ‫ޕ‬↳⺧ᯏ㑐߆ࠄߩታ࿾⺞ᩏᤨߩ෻ᔕߣߒߡߪ‫⥄ޔ‬ਥ▤ℂߦ㑐ߔࠆታ࿾⺞ᩏߪೋ߼ߡߢ޽
ࠅኻᔕᣇᴺ߇ࠊ߆ࠄߥ޿ߣߩࠦࡔࡦ࠻߇ᄙߊߺࠄࠇߚ߽ߩߩ‫⹏⸽⹺ޔ‬ଔຬ߆ࠄߩࠦࡔࡦ
࠻ߦߟ޿ߡ‫ޔ‬ᄖㇱ߆ࠄߩⷞὐߦߟ޿ߡߩ᳇ߠ߈ߣߒߡߣࠄ߃ߡ߽ࠄ߃ߚߣ⠨߃ߡ޿ࠆ‫ޕ‬߹ߚ‫ޔ‬ฦ↳⺧⠪ߣ߽ၮᧄᜰ㊎ߦᓥߞߡ‫⥄ޔ‬ਥ▤ℂ૕೙ߪㆇ↪ߐࠇߡ޿ࠆ߽ߩߩ‫ޔ‬ᜰ㊎೙ቯ೨ߩⷙቯ߿ㆇ↪ࠍၮ⋚ߣߒߡ޿ࠆߣᕁࠊࠇ‫ޔ‬ᯏ㑐ౝⷙ⒟ߩ↪⺆߿⸥タ߱ࠅߦߟ޿ߡ‫ޔ‬ℂ⸃ߒ߿ߔߐߩὐ߆ࠄࠃࠅలታࠍ᳞߼ࠆ႐ว߽޽ࠆ‫ޕ‬
㧠㧕2 ࿁⋡ߩ⹺ቯߦ߅ߌࠆ⺞ᩏߦߟ޿ߡ ᐔᚑ 23 ᐕᐲਅඨᦼߦ߅޿ߡ‫ޔ‬೙ᐲ㐿ᆎ 1 ᐕ⋡㧔ᐔᚑ 20 ᐕᐲ㧕ߦ⹺⸽ߒߚᣉ⸳ߦߟ޿
ߡ‫╙ߩ߼ߚߩ⸽⹺⛯⛮ޔ‬ੑ࿁ߩ⺞ᩏ߇ታᣉߐࠇࠆߎߣߣߥࠅ‫ޔ‬2 ࿁⋡ߩታ࿾⺞ᩏߩᣇᴺ‫ޔ‬⹏ଔߩⷞὐߦߟ޿ߡ㨮⹏ଔᆔຬળ෸߮ㆇ༡ᆔຬળߦ߅޿ߡᬌ⸛ࠍታᣉߒߚ‫ޕ‬ ଀߃߫‫ޔ‬ೋ࿁ߩ⹏ଔᤨߦ⹏ଔ⚿ᨐႎ๔ᦠߣߒߡવ߃ߡ޿ࠆ⥄ਥ▤ℂߩะ਄ߦ߻ߌߡߩ

ኻᔕ⁁ᴫߩ⏕⹺ߪᔅ㗇ߢ޽ࠆ߇‫ޔ‬೨࿁⹏ଔኻ⽎ߣߒߚ੐ታ㑐ଥࠍౣᐲ⏕⹺ߔߴ߈߆ุ߆
ߦߟ޿ߡᬌ⸛ࠍⴕߞߚ‫ޕ‬ᣉ⸳ߩㆇ↪ߩᄌᦝ߿␠ౝ૕೙ߩᄌᦝ╬ߩน⢻ᕈ߇޽ࠆߎߣ߆ࠄ‫ޔ‬ೋ࿁ߩ⺞ᩏߣหߓࠃ߁ߦᯏ㑐ౝⷙ⒟ߩ⏕⹺‫ޔ‬േ‛ታ㛎ᆔຬળߩᵴേ⁁ᴫ‫⥄ޔ‬Ꮖὐᬌ‫ޔ‬ᢎ⢒⸠✵‫ޔ‬ᖱႎ౏㐿‫ࠆࠃߦ࡯ࠕ࠷ࡏ࡜ޔ‬㘺㙃଻▤⁁ᴫߩ⏕⹺╬ࠍⴕ߁ߎߣߣߒߚ‫ޕ‬ ታ㓙ߦ 2 ࿁⋡ߩታ࿾⺞ᩏߢߪ‫ޔ‬೨࿁⺞ᩏᤨߩ⥄ਥ▤ℂߩะ਄ߦะߌߡߩࠦࡔࡦ࠻ߦኻ
ߒߡ‫ޔ‬ฦᯏ㑐ߢߪ⌀៼ߦኻᔕ޿ߚߛ߈‫⥄ޔ‬ਥ▤ℂߩะ਄ߦߟߥ߇ࠆ⚿ᨐߣߥߞߡ޿ߚ‫ޕ‬߹ߚ‫ޔ‬ᯏ㑐ౝⷙ⒟ߩᄌᦝ‫ޔ‬ᆔຬળߩክᩏㆇ↪ߩᄌᦝ‫▸ߩ⸽⹺ޔ‬࿐ߩᄌᦝ㧔േ‛㘺⢒ᣉ⸳ߩ㐽㎮‫ޔ‬ᣉ⸳ߩㆇ↪ߩᄌᦝ㧔ࡃ࡝ࠕ߆ࠄࠦࡦࡌࡦ࡚ࠪ࠽࡞߳㧕ߦ઻߁߽ߩ╬㧕ߥߤ߇޽ࠅ‫ޔ‬2 ࿁⋡ߩታ࿾⺞ᩏߦ߅޿ߡ߽ೋ࿁ߣห᭽ߦ⺞ᩏࠍⴕ߁ߎߣߪᗧ⟵޽ࠆ⁁ᴫߣ⹺⼂ߐࠇߚ‫ޕ‬ ․ߦ‫ޔ‬቟ᭉᱫᴺ‫ޔ‬㤗㈮ᴺߩᄌᦝ‫ޔ‬㘺⢒ⅣႺߩᡷༀߥߤേ‛⑔␩ߦ㑐ߔࠆ࿖㓙േะ߳ߩኻᔕߪฦᣉ⸳ߦ߅޿ߡචಽ⹺⼂ߐࠇ‫ޔ‬ኻᔕ߇ㅴࠎߢ޿ߚߎߣࠍᒝ⺞ߒߚ޿‫ޕ‬߅ࠊࠅߦᶏᄖߩⷙ೙ࠍߺߡ߽☨࿖‫ޔ‬᰷Ꮊߢ⇣ߥࠅ‫ޔ‬࿾ၞߦහߒߚ▤ℂᣇᴺ߇ㆬᛯߐࠇ‫ޔ‬ታᣉߐࠇߡ޿ࠆ߇‫ޔ‬േ‛ታ㛎ࠍᎼࠆⅣႺߩᄌൻߪᐕࠍ߅ߞߡᄢ߈ߊߥߞߡ޿ࠆࠃ߁ߦᗵߓࠄࠇ
ࠆ‫ޕ‬ᚒ߇࿖ߩേ‛ታ㛎ߦ㑐ߔࠆⷙ೙ߩᨒ⚵ߺߩ⼏⺰ߪ‫ޔ‬ᰴ࿁ߩേ‛ᗲ⼔ᴺߩᡷᱜᤨᦼߦ
ะߌߡᣢߦᆎ߹ߞߡ޿ࠆߣ޿߃ࠃ߁‫ޕ‬ᰴ࿁ᡷᱜߩ 4 ᐕᓟߦ߻ߌߡ‫ޔ‬േ‛ታ㛎ߩ⥄ਥ▤ℂ⁁ᴫߦߟ޿ߡ㑐ଥ⠪㑆ߢߩචಽߥᖱႎ౒᦭ߣ⼏⺰ߩᷓ߹ࠅ߇ᔅⷐߣ⠨߃ࠆ‫ޕ‬
⽷࿅ߣߒߡ߽‫ޔ‬േ‛ታ㛎ታᣉᣉ⸳ᄖㇱ⹏ଔ੐ᬺࠍㅢߓߡ‫ޔ‬ㆡᱜߥേ‛ታ㛎ߩታᣉߦ㑐ߔࠆ㑐ଥ⠪ߩℂ⸃߇ᷓ߹ࠆࠃ߁ദജߒߡ޿ߊᚲሽߢ޽ࠆ‫ޕ‬ෳ⠨⾗ᢱ㧝㧚േ‛ߩᗲ⼔෸߮▤ℂࠍ߼ߋࠆ⃻⁁ߣ⺖㗴㧔ᐔᚑ 24 ᐕ 8 ᦬ ⴐ⼏㒮⺞ᩏዪⅣႺ⺞ᩏቶ㧕
http://www.shugiin.go.jp/itdb_rchome.nsf/html/rchome/Shiryo/kankyou_201208_dob
utsuaigo.pdf/$File/kankyou_201208_dobutsuaigo.pdf
㧞㧚ⴐ⼏㒮ⅣႺᆔຬળ᳿⼏㧔⼏੐㍳㧕
http://www.shugiin.go.jp/itdb_kaigiroku.nsf/html/kaigiroku/001718020120828012.htmhttp://www.shugiin.go.jp/index.nsf/html/index_rchome.htm
㧟㧚ෳ⼏㒮㒝Ꮺ᳿⼏
http://www.sangiin.go.jp/japanese/gianjoho/ketsugi/180/f073_082801.pdf
㧠㧚േ‛ߩᗲ⼔෸߮▤ℂߦ㑐ߔࠆᣉ╷ࠍ✚ว⊛ߦផㅴߔࠆߚ߼ߩၮᧄ⊛ߥᜰ㊎㧔ᛞ㧕ᐔᚑ 18 ᐕⅣႺ⋭๔␜╙ 140 ภ㧔ᦨ⚳ᡷᱜ㧦ᐔᚑ 25 ᐕⅣႺ⋭๔␜╙ 80 ภ㧕
╙ 2 ੹ᓟߩᣉ╷ዷ㐿ߩᣇะ㧞ᣉ╷೎ߩข⚵ߺ㧔㧢㧕ታ㛎േ‛ߩㆡᱜߥขᛒ޿ߩផㅴ
Ԙ⃻⁁ߣ⺖㗴
ታ㛎േ‛ߩ㘺㙃╬ߦߟ޿ߡߪ‫ޔ‬ታ㛎േ‛ߩ㘺㙃෸߮଻▤ਗ߮ߦ⧰∩ߩシᷫߦ㑐ߔࠆၮḰ㧔ᐔᚑ 18 ᐕ 4 ᦬ⅣႺ⋭๔␜╙ 88 ภ‫ޔ‬એਅ㨬ታ㛎േ‛ߩ㘺㙃଻▤╬ၮḰ㨭ߣ޿߁‫ޕ‬㧕ߦၮߠ߈‫⥄ޔ‬ਥ▤ℂࠍၮᧄߣߒߡߘߩㆡᱜൻࠍ࿑ࠆ઀⚵ߺߣߥߞߡ޿ࠆ߇‫ᧄޔ‬ၮḰߩㆩ቞ߒห╬ࠍ౞Ṗߦⴕ߁ߚ߼ߩ૕೙ᢛ஻߇චಽߦߐࠇߡ޿ߥ޿ᣉ⸳߇৻ㇱߦ޽ࠆ‫ޕ‬േ‛ࠍ⑼ቇ
਄ߩ೑↪ߦଏߔࠆߎߣߪ‫↢ޔ‬๮⑼ቇߩㅴዷ‫ޔ‬ක≮ᛛⴚ╬ߩ㐿⊒╬ߩߚ߼ߦᔅⷐਇนᰳߥ
߽ߩߢ޽ࠆ߇‫ߩߘޔ‬㘺㙃෸߮⑼ቇ਄ߩ೑↪ߦᒰߚߞߡߪ‫ޔ‬േ‛߇๮޽ࠆ߽ߩߢ޽ࠆߎߣߦ㐓ߺ‫⑼ޔ‬ቇ਄ߩ೑↪ߩ⋡⊛ࠍ㆐ߔࠆߎߣ߇ߢ߈ࠆ▸࿐ߦ߅޿ߡ‫ޔ‬࿖వ⊛ߦ߽᥉෸ߒ‫ޔ‬ቯ⌕ߒߡ޿ࠆታ㛎േ‛ߩขᛒ޿ߩၮᧄ⊛⠨߃ᣇߢ޽ࠆ‫ޟ‬㧟R ߩේೣ‫ޠ‬
㧔ઍᦧᴺߩᵴ↪㧦
Replacement‫↪૶ޔ‬ᢙߩ೥ᷫ㧦Reduction‫ߩ∩⧰ޔ‬シᷫ㧦Refinement㧕ࠍ〯߹߃ߚㆡಾ
ߥភ⟎ࠍ⻠ߓࠆߎߣ╬߇ᔅⷐߣߐࠇߡ޿ࠆ‫ޕ‬ԙ⻠ߕߴ߈ᣉ╷ࠕ㑐ଥ⋭ᐡ‫ޔ‬࿅૕╬ߣㅪ៤ߒߟߟ‫ޔ‬‫ޟ‬㧟R ߩේೣ‫ޠ‬߿ታ㛎േ‛ߩ㘺㙃଻▤╬ၮḰߩ๟⍮߇‫ޔ‬ᒰ⹥ၮḰߩ⸃⺑ᦠߩ૞ᚑ╬ࠍㅢߒߡലᨐ⊛߆ߟല₸⊛ߦⴕࠊࠇࠆࠃ߁ߦߔࠆߣߣ߽ߦ‫ޔ‬ታ㛎േ‛ߦ㑐ߔࠆ࿖㓙⊛ߥⷙ೙ߩേะ߿⑼ቇ⊛⍮⷗ߦ㑐ߔࠆᖱႎࠍ෼㓸ߔࠆߎߣ‫ޕ‬ࠗ࿖ߪ‫ޔ‬ታ㛎േ‛ߩ㘺㙃଻▤╬ၮḰߩㆩ቞⁁ᴫߦߟ޿ߡ‫✕ޔ‬ᕆᤨߦኻᔕߔࠆߚ߼ߩ⸘↹૞ᚑ⁁ᴫ߽฽߼‫ޔ‬ቯᦼ⊛ߥታᘒᛠីࠍⴕ߁ߎߣ‫ޕ‬⤖፧ࡋᏊ࡝ࡶࡶ࡛ࡁ࡚ࡸࡀ࡚⮫ᗋ⋇་እ⛉་࡟ࡶᡠࢁ࠺࠿࡜ᛮࡗ࡚࠸
ࡓ▮ඛࠊ኱㜰኱Ꮫ་Ꮫ㒊࡬⾜ࡃヰࡀฟࡓࠋ᪂ࡋࡃື≀ᐇ㦂᪋タࢆసࡿࡢ࡛
ࡑࡢ㈐௵⪅࡜ࡋ࡚㌿௵ࡍࡿࡇ࡜࡜࡞ࡗࡓࠋ኱㜰኱Ꮫ࡟⾜ࡃࡲ࡛ࡢ㛫ࠊᐇ୰◊ࡢ㔝ᮧ㐩἞ඛ⏕ࠊ㘽ᒣ┤Ꮚඛ⏕ࡢࡈᣦᑟࢆ࠺ࡅࡿࡇ࡜࡜࡞ࡾࠊ⮬ศࡀ඲ࡃ⤒㦂ࡋࡓࡇ࡜ࡢ࡞࠸ᚤ⏕≀ࣔࢽࢱࣜࣥࢢࡸ⏕ṪᕤᏛ࡞࡝ࢆ࡟ࢃ࠿࡟ຮ
ᙉࡋࡓࡇ࡜ࢆ࡞ࡘ࠿ࡋࡃᛮ࠸ฟࡍࠋఀ⸨Ⓩᚿ㞝ඛ⏕࡟ࡣ࠾㓇ࡢ㣧ࡳ᪉ࢆࡓࡗ࡫ࡾఏᤵࡋ࡚ࡶࡽࡗࡓࠋࡲࡓᐇ୰◊ࡢ௰㛫࡜㯞㞛ࠊࢸࢽࢫ࡟⯆ࡌࡓࠋ◊✲⏕άࡢ᮰ࡢ㛫ࡢࡃࡘࢁࡂ࡛࠶ࡗࡓࠋ኱㜰኱Ꮫ㉱௵ᚋࡣື≀ᐇ㦂᪋タᘓタࡢࡓࡵ࡯ࡰᅵ᪉ࡢぶ᪉࡜ྠࡌࡼ࠺࡞⏕ά࡜࡞ࡾࠊᘓ⠏タィࠊタഛタィ࡞࡝ࢆ୍࠿ࡽぬ࠼࡞ࡀࡽࠊᴫᛕタィࢆ⾜ࡗࡓࠋࡇࡢ࡜ࡁ኱㜰኱Ꮫࡣ⡿ᅜᆺࡢᐇ㦂ື≀⟶⌮ࢆ⾜࠺ࡇ࡜࡜ࡋࠊ⚾ࡣ⡿ᅜ࣑ࢩ࢞ࣥ኱Ꮫࡢ Ben Cohen ඛ⏕࡟ᖌ஦ࡍࡿࡇ࡜࡜࡞ࡗࡓࠋࡑࡇ࡛ᐇ㦂ື≀་ᏛࡢᏑᅾࢆ▱ࡾࠊⱝᡭࡢᐇ㦂ື≀་Ꮫᑓ㛛་ከᩘࡢ▱ᕫࢆᚓࡿࡇ࡜࡜࡞ࡗࡓࠋᡃࡀᅜ࡟ࡶ㐺ษ࡞ᐇ㦂ື≀⚟♴ࡢᯟ⤌ࡳࡀᚲせ࡛࠶ࡿ࡜⪃࠼⡿ᅜࡢ௙⤌ࡳ࡟⩦࠾࠺࡜ࡋࡓࡀᡃࡀᅜ
࡟ࡣࡇࡢᑓ㛛་ไᗘࡀ࡞࠸ࡇ࡜ࡀࢿࢵࢡࡢࡼ࠺࡟ᛮࢃࢀࠊ᪥ᮏᐇ㦂ື≀་Ꮫ఍ࡢタ❧࡟➟஭᠇㞷ඛ⏕࡜ዑ㉮ࡋࡓࠋ⌧ᅾࡇࡢᑓ㛛་ไᗘࡣ㡰ㄪ࡟Ⓨᒎࡋࠊ96 ྡࡢᑓ㛛་ࢆㄆᐃࡋࡓࡔࡅ࡛࡞ࡃᅜ㝿⤌⧊࡛࠶ࡿ IACLAM ࡢ࣓ࣥࣂ࣮࡜࡞ࡿࡇ࡜ࡀ࡛ࡁࡓࠋ
᪂㗦ࡢ᪋タࡶฟ᮶ୖࡀࡾࠊ㐠Ⴀࡢࢯࣇࢺࡶᅛࡲࡗࡓࡇࢁᐤ⏕⹸Ꮫ⪅࡛࠶ࡿᒸᮏ᐀⿱ຓᡭࡀ㉱௵ࡋࡓࠋື≀ᐇ㦂᪋タࡢ㐠Ⴀࡶࡉࡿࡇ࡜࡞ࡀࡽࠊඹ㏻
ࡢ◊✲ࢸ࣮࣐ࢆ᥈ࡍࡇ࡜࡜࡞ࡾࠊ⭈⮚⑓ࢆࢸ࣮࣐࡜ࡍࡿࡇ࡜࡜ࡋࡓࠋண◊࠿ࡽ୰ᕝ㞞㑻ඛ⏕ࡽࡀ㛤Ⓨࡋࡓ ICGN ࣐࢘ࢫࢆ࠸ࡓࡔ࠸࡚ࠊ◊✲ࢆ㛤ጞࡋࡓࠋ⌧ᅾࡇࡢ៏ᛶ⭈୙඲Ⓨ⑕ࣔࢹ࣐ࣝ࢘ࢫࡣ F63 ࡟࡞ࡗ࡚࠸ࡿࠋ㏵୰Ỉ㔝ಙဢྩ࠾ࡼࡧࡸࡀ࡚ᙼ࡜⤖፧ࡍࡿᇼᕝὒᏊྩࠊ୰ᅜ࠿ࡽࡁࡓᓅ⛃梆ྩࡶ◊✲ᐊ࡟ຍࢃࡾࠊ୍᫬ᮇࡣࡑࡢ◊✲࡟ἐ㢌ࡋࡓࠋ◊✲ᐊဨࡢດຊ࡛ࡑࢀ࡞ࡾ
ࡢ◊✲ᡂᯝࡶ࠶ࡆࡿࡇ࡜ࡀ࡛ࡁࡓࠋ
ࡋ࠿ࡋࠊࡑࡢ㛫࡟ࡶື≀⚟♴ࡢ◊✲ࡣ㐍ࡵ࡚࠸࡚ࠊ๓ᓥඛ⏕ࡢඛᑟ࡛
Koken Rat ࡢ㛤Ⓨ࡟ᦠࢃࡾࠊࡑࡢᬑཬ࡟ດຊࡋࡓࠋࡇࢀࢆࡁࡗ࠿ࡅ࡟᪥ᮏ
ື≀ᐇ㦂௦᭰ἲᏛ఍࡟ධ఍ࡋࠊࡸࡀ࡚✀ࠎࡢᙺဨࢆ௮ࡏࡘ࠿ࡿࡇ࡜࡜࡞ࡗ
ࡓࠋࡇࡢື≀ᐇ㦂௦᭰ἲᏛ࡟ᦠࢃࡿ๓ာᡓ࡜ࡋ࡚ ISO/TC194 WG3

Animal Protection Aspect ࡢ᪥ᮏ௦⾲࡜࡞ࡾ ISO ᩥ᭩ Animal Welfare
Requirement ࢆసࡗࡓࡇ࡜ࡀ่⃭࡜࡞ࡗ࡚࠸ࡓࠋື≀ᐇ㦂௦᭰ἲᏛࡣ㸱Rs ࡟࠿࠿ࢃࡿ◊✲࡜ᐇ㊶࡛࠶ࡿࡀࠊᏛ఍ࡣࡶࡗࡥࡽ Replacement ࡢ◊✲ࢆ࠾ࡇ࡞ࡗ࡚࠸࡚ࠊRefinement ࡢ◊✲ࡣ୺ὶ࡛ࡣ࡞࠿ࡗࡓࠋ2007 ᖺ࡟
WC6 ୡ⏺ື≀ᐇ㦂௦᭰ἲ఍㆟ࢆ኱㔝Ὀ㞝఍㛗ࡢࡶ࡜ᮾி࡛㛤ദࡍࡿࡇ࡜
࡜࡞ࡾࠊ⛉Ꮫጤဨ㛗ࢆᣏ࿨ࡋࠊୡ⏺ࡢື≀ᐇ㦂௦᭰ἲࢆ┠ࡢᙜࡓࡾ࡟ࡳࡿ
ࡇ࡜࡜࡞ࡾࠊRefinement ࡀᡃࡀᅜࡢ࢘࢖࣮ࢡ࣏࢖ࣥࢺ࡛࠶ࡿࡇ࡜ࢆᛮ࠸
▱ࡽࡉࢀࡓࠋRefinement ࡢ඘ᐇࡣ⋇་Ꮫⓗࢣ࢔ࡀ୰ᚰ࡜࡞ࡗ࡚࠸ࡿࡇ࡜
࠿ࡽࠊື≀ᐇ㦂௦᭰ἲᏛ࡜ᐇ㦂ື≀་Ꮫࡀᐦ᥋࡟⤖ࡧࡘ࠸࡚࠸ࡿࡇ࡜ࡀ⌮ゎ࡛ࡁࡓࠋࡋࡓࡀࡗ࡚ᙜึ࠿ࡽ⾜ࡗ࡚࠸ࡓᐇ㦂ື≀㯞㓉Ꮫࡢ◊✲ࡶࡑࡢ୧⪅࡟ࡲࡓࡀࡗ࡚࠸ࡿࡇ࡜࡟Ẽ௜࠸ࡓࠋࡲࡓ Refinement ࡢᐇ㊶࡜ࡋ࡚ࡣ
AAALAC International ࡢᏑᅾࡀ࠶ࡾࠊᐇ㦂ື≀ࡢ฼⏝࡜⚟♴ࡢホ౯ㄆᐃࢆ⾜࠺ࡇ࡜ࢆ㏻ࡌ࡚ᐇ㦂ື≀⚟♴ࡢ඘ᐇ࡜◊✲ࡢ᥎㐍ࡀᅜ㝿ⓗ࡟⾜ࢃࢀ࡚࠸ࡿࡇ࡜ࡶ⌮ゎࡍࡿࡼ࠺࡟࡞ࡗࡓࠋ
ࡇ࠺ࡋ࡚ࠊ⋇་እ⛉་ࡀᐇ㦂ື≀Ꮫ࡟ධࡾ㎸ࡳᐇ㦂ື≀་Ꮫᑓ㛛་఍ࡢタ❧࡟ዑ㉮ࡋࠊࡉࡽ࡟ࡣື≀ᐇ㦂௦᭰ἲᏛ࡟ࡲ࡛ࡑࡢ◊✲㡿ᇦࢆᗈࡵ࡚࠸
ࡗࡓࡢ࡛࠶ࡿࠋࡲࡓࡇࢀࡽࡢάືࡣᡃࡀᅜࡔࡅ࡟࡜࡝ࡲࡽࡎࠊࡸࡀ࡚
AFLAS ࠾ࡼࡧ IACLAM ࡢタ❧࡬࡜ࡘ࡞ࡀࡗ࡚࠸ࡗࡓࡢ࡛࠶ࡿࠋ
ᐃᖺࢆ㏄࠼࡚ࡍ࡭࡚ࡢ Duties ࠿ࡽ㏨ࢀࡽࢀࡿࡇ࡜ࢆክぢ࡚࠸ࡓࡀࠊࡇࢀ
ࡽࡢάືࡢ୰࡟ࡣ࠸ࡲࡔᮍ᏶ᡂࡢࡶࡢࡀ࠶ࡿࡼ࠺࡟ឤࡐࡽࢀࠊࡶ࠺ࡕࡻࡗ
࡜ࡔࡅࡣ࠾௜ࡁྜ࠸ࡋࡼ࠺࡜࠸࠺Ẽᣢࡕ࡟࡞ࡗ࡚࠸ࡿࠋ

ታ㛎േ‛ߩ㗔ၞߦẜࠅㄟࠎߢᓧߚ߽ߩ
⧔Ꮉᔘᄦ㧔੩ㇺᄢቇකቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳㧕ߪߓ߼ߦᄢ㒋ᐭ┙ᄢቇㄘቇㇱ₞කቇ⑼ߦ౉ቇߒߡએ᧪‫ޔ‬₞කᏧߣߒߡߩㅴ〝ࠍ޽ࠇߎࠇ⠨߃ߚ‫ޕ‬ᒰᤨ‫ޔ‬₞කቇ⑼ߦߪታ㛎േ‛ቇᢎቶߪߥߊ‫ޔ‬ታ㛎േ‛ቇߣ޿߁᝼ᬺ߽ߥ߆ߞߚ‫ޔߒ߆ߒޕ‬තᓟߩㆬᛯ⢇ࠍ޿ࠈ޿ࠈᬌ⸛ߒߡ޿ࠆ߁ߜߦ‫ޔ‬ⁿ ⾆⟵వ↢߿ጊౝᔘᐔవ↢ࠄ߇‫⎇ߩ↥⇓ޟ‬ⓥ‫ޠ‬
㧔26 Ꮞ╙ 1 ภ‫ޔ‬ᤘ๺ 47 ᐕ 1 ᦬⊒ⴕ‫ޔ‬㙃⾫ၴ㧕ߦၫ╩ߐࠇߡ޿ߚታ㛎േ‛ቇߦ㑐ߔࠆ✚⺑ߦ಴ળߞߚ‫⊒ޕ‬ዷㅜ਄ߩಽ㊁ߢ޽ࠆߎߣࠍ⍮ࠅ‫߽ߦ⑳ޔ‬૗߆ߢ߈ߘ߁ߥ㝯ജࠍᛴ߆ߐࠇ
ߚ‫ޔߡߒߘޕ‬ታ㛎േ‛ߩ㗔ၞߦりࠍ⟎ߊߎߣࠍ᳿߼ߡ‫ޔ‬࿾రߩ੩ㇺߦᧄ␠ߩ޽ࠆർጊ࡜ࡌ
ࠬᩣᑼળ␠ߦዞ⡯ߒߚ‫⿞ޕ‬છవߪጘ㒂⋵ߦ޽ߞߚ⠀ፉ⎇ⓥᚲߢ SPF ࡜࠶࠻ߩ↢↥ߣ࡜࠶࠻ࠍ↪޿ߚ቟ోᕈ⹜㛎ߩ⷗⠌޿߆ࠄࠬ࠲࡯࠻ߒߚ‫ޕ‬ඨᐕᓟ‫ޔ‬࿖┙੍㒐ⴡ↢⎇ⓥᚲ㧔੍⎇㧕ߩ₞∉ㇱታ㛎േ‛ቶߦ߅޿ߡታ㛎േ‛ߩᗵᨴ∝ߦߟ޿ߡߩ⎇ୃࠍߘߩᐕᐲᧃ߹ߢߐߖߡ㗂޿ߚ‫ޕ‬ਛ㊁ஜมవ↢‫ޔ‬ᱞ⮮ ஜవ↢‫ޔ‬ਛᎹ㓷ᦶవ↢ࠄߩᜰዉࠍฃߌߚ‫ޕ‬ᒰᤨ‫⎇੍ޔ‬₞∉ㇱߩታ㛎േ‛ቶߦߪᄙߊߩታ㛎േ‛㑐ㅪᬺ⠪ߩ․ߦ⧯ᚻ߇⎇ୃߦ಴౉ࠅߒߡ߅ࠅ‫߁ࠃߩࠞ࠶ࡔޔ‬ߥሽ࿷ߢ޽ߞߚ‫ޕ‬㑐⷏ታ㛎േ‛⎇ⓥળߩ⃻ળຬߢ޽ࠆᤊ㊁㓷ⴕߐࠎ߿ᧁਅ㇌᣿ߐࠎ߽‫ߘޔ‬ߩਛߦ߅ࠄࠇߚ‫∛ߩࠦࡀޔࠡࠨ࠙ޔ࠻࠶ࡕ࡞ࡕޔ࠻࠶࡜ޔࠬ࠙ࡑߪ⑳ޕ‬ℂ⸃೬ᬌᩏ߿ነ↢⯻ᬌᩏߦട߃ߡ‫ߩࠡࠨ࠙ޔ‬⢄⤳ဳࠦࠢࠪࠫ࠙ࡓߩᗵᨴታ㛎ࠍ⚻㛎ߐߖߡ㗂޿ߚ‫ޕ‬⠀ፉ⎇ⓥᚲߦᚯࠅᬺോߦ៤ࠊߞߡ޿ߊਛߢ‫ߩ࠻࠶࡜ޔ‬⣺࠻࡝ࠦࡕ࠽ࠬ߿ᒰᤨ࿖ౝߢ⊒⴫ႎ๔ߩߥ߆
ߞߚ‫ޔ‬ᚲ⻐࡜࠶࠻ߩ߅ߚ߰ߊ㘑㇎㧔ໜᶧ⣼ᶡ⣼Ἳ࠙ࠗ࡞ࠬᗵᨴ∝㧕ࠍ⷗ߟߌߡߒ߹޿‫ޔ‬SPF
ࠦࡠ࠾࡯ߩ⛽ᜬߩ㔍ߒߐߣኻ╷ߩ㔍ߒߐࠍ∩ಾߦᗵߓߚ‫ޕ‬㕙⊕ߐ߽޽ࠅ‫ޔ‬૗߆ߐࠄߦᄢ߈
ߊዷ㐿ߒߚ޿ߣ޿߁りൎᚻߥᗐ޿߇ߤࠎߤࠎ⤘ࠄࠎߢ߽ⴕߞߚ߇‫⃻ޔ‬ታߣߩࠡࡖ࠶ࡊࠍస
᦯ߔࠆߎߣ߇ߢ߈ߕߦ‫ޔ‬ᒰߡ߽ߥߊㆀߦળ␠ࠍ෰ߞߡߒ߹ߞߚ‫ޔߒ߆ߒޕ‬੩ㇺߩ⥄ቛߢߩ 2߆᦬㑆ߩᄬᬺ↢ᵴߩᓟߦ‫ߩ⎇੍ޔ‬వ↢ߦ⚫੺ߐࠇߡ੩ㇺᄢቇකቇㇱ㒝ዻേ‛ታ㛎ᣉ⸳ߩഥᚻߦᔕ൐ߒߚߣߎࠈ‫ޔ‬ᕁ޿߇ߌߥߊណ↪ߐࠇߚ‫ޕ‬̈ታ㛎േ‛ߩ㗔ၞߦẜࠅㄟࠎߢ…̉ߣ޿߁࠲ࠗ࠻࡞ߪ‫ߦߣߎߚߞ޽߇✲⚻ߥ߁ࠃߩߎޔ‬࿃ࠆ‫ޕ‬㧝㧚੩ㇺᄢቇߦ߅ߌࠆേ‛ታ㛎ߩ▤ℂᬺോ㒝ዻേ‛ታ㛎ᣉ⸳ߩഥᚻߣߒߡᤘ๺  ᐕ  ᦬ߦ⿞છ‫ޔ‬ᤘ๺  ᐕ㧝᦬ߦഥᢎ᝼‫ޔ‬ᐔᚑ㧡ᐕ㧥᦬ߦᢎ᝼‫ߡߒߘޔ‬ᐔᚑ㧢ᐕ㧠᦬એ㒠‫ޔ‬ᐔᚑ  ᐕ㧟᦬ߦቯᐕㅌ⡯ߔࠆ߹ߢേ‛ታ㛎ᣉ⸳㐳ࠍ૬છߒߚ‫ޔߚ߹ޕ‬੩ㇺᄢቇߩേ‛ታ㛎ᆔຬળߩᆔຬ㐳‫ޔ‬೽ᆔຬ㐳‫ޔ‬කቇ⎇ⓥ⑼ߩേ‛ታ㛎ᆔຬળߩᆔຬ㐳߽㐳ߊᜂߞߚ‫  ⚂ޕ‬ᐕ㑆߽ᐢ⟵ߩ౏⡯ࠍോ߼ߚߩߢ޽ࠆ߆ࠄ‫⸥ޔ‬㍳ࠍ
ᱷߒߡ߅ߊ⟵ോ߇޽ࠆߣᕁ޿‫ߡߖࠄ✄ߦ⚦⹦ޔ‬㗂ߊ‫ޕ‬㧝㧕േ‛ታ㛎ᣉ⸳ߩ⸳┙‫ޔ‬ᑪ⸳‫ޔ‬ᚻ⋥ߒᎿ੐‫ߡߒߘޔ‬ౣⒿ௛ᤘ๺  ᐕ  ᦬  ᣣߦ㒝ዻേ‛ታ㛎ᣉ⸳߇⸳⟎ߐࠇ‫↢ⴡޔ‬ቇߩ⮮ේరౖᢎ᝼߇ᣉ⸳㐳ߦዞછ‫✚ޔ‬Ꮏ⾌⚂  ం౞‫ޔ‬ᑧᐥ㕙Ⓧ  ᐔᣇࡔ࡯࠻࡞ߩᧄ᫟ߩᑪ⸳߇⹺นߐࠇߚ‫ޕ‬ᣥ㧣Ꮲᄢߩකቇㇱߦേ‛ታ㛎ᣉ⸳߇⺀↢ߒᆎ߼ࠆೋᦼߩ㗃ߢ޽ࠆ‫ޕ‬ᣧㅦߦ‫┙⸳ޔ‬ᆔຬળ߇⸳⟎ߐࠇ‫ޔ‬කቇ⎇ⓥ⑼ߩၮ␆ߣ⥃ᐥߦዻߔࠆᢎຬ߿ᛛቭ߇ᆔຬߦㆬ߫ࠇ‫ޔ‬േ‛ታ㛎ᣉ⸳᫟߇⸳⸘ߐࠇ
ߚ‫ޕ‬⠉ᐕߩᤘ๺  ᐕ  ᦬ߦ‫ޔ‬ጊ↰ᷕਃవ↢߇᡼኿✢කቇ✚ว⎇ⓥᚲ߆ࠄኾછߩഥᢎ᝼ߣߒ
ߡ⿞છߐࠇ‫ޔ‬ห᦬ߦേ‛ታ㛎ᣉ⸳᫟߇⌕Ꮏߐࠇߚ‫ߪ⑳ޕ‬หᐕ  ᦬ណ↪ߢ޽ߞߚ‫ޔߦ߃ࠁޕ‬⑳㆐ኾછᢎຬ  ฬߪ⸳⸘ߦଥࠊࠄߥ߆ߞߚ‫ޕ‬᳞߼ࠄࠇߚߩߪ‫ޔ‬ᑪ▽ㅜ਄ߦ߅ߌࠆ⺞ᢛߣቢᚑᓟߩേ‛ታ㛎ᣉ⸳᫟ߣ⚐♽േ‛㘺⢒ቶߩㆇ༡߇ਥߥ߽ߩߢ޽ߞߚ‫♽⚐ޕ‬േ‛㘺⢒ቶߢߪ‫ޔ‬ᤘ๺  ᐕએ᧪‫ޔ‬ㄭ੤♽ߣ㕖ㄭ੤♽ߩࡑ࠙ࠬ߅ࠃ߮੩ㇺ࠙ࠗࠬ࠲࡯࡜࠶࠻ߩ⛽ᜬ‫❥ޔ‬ᱺ‫ޔ‬ឭଏ੐ᬺ߇ⴕࠊࠇߡ޿ߚ‫᧲ޕ‬ർᄢቇ‫ޔ‬ᄢ㒋ᄢቇ‫਻ޔ‬Ꮊᄢቇߥߤ‫ઁޔ‬ᄢቇߩකቇㇱߦ߅޿ߡ߽‫ޔ‬ታ㛎↪ࡑ࠙ࠬ߅ࠃ߮࡜࠶࠻ߩቇౝଏ⛎੐ᬺ߇ห᭽ߦⴕࠊࠇߡ޿ߚ‫ޕ‬ᤘ๺  ᐕ㗃߹ߢߦߪ‫ޔ‬∛ℂቇᢎቶߦ߅޿ߡ੩ㇺ࠙ࠗࠬ࠲࡯࡜࠶࠻߆ࠄㆬᛮ⢒⒳ᴺߦࠃࠅ㜞ⴊ࿶⥄ὼ⊒∝࡜࠶࠻
5*4 ߇㐿⊒ߐࠇߡ޿ߚ‫ޕ‬ᤘ๺  ᐕ  ᦬ߦ‫ޔ‬ታⴕዊᆔຬળߣ⒓ߒߡ‫ޔ‬㧔㧝㧕ࡑ࠙ࠬ‫ޔ࠻࠶࡜ޔ‬ࡂࡓࠬ࠲࡯ᆔຬળ‫ޔ‬㧔㧞㧕࠙ࠨࠡ‫࠻࠶ࡕ࡞ࡕޔ‬ᆔຬળ‫ޔ‬㧔㧟㧕ࠗ࠿‫࡞ࠨޔࠦࡀޔ‬ᆔຬળ‫ޔ‬㧔㧠㧕ή⩶‫❥ޔ⛔♽ޔ‬ᱺᆔຬળ‫ޔ‬㧔㧡㧕ⷙ⒟૞ᚑᆔຬળ‫ޔ‬㧔㧢㧕ᢛ஻⸘↹ᆔຬળ‫ޔ‬㧔㧣㧕ㆇ༡ᆔຬળ‫ޔ‬߇⊒⿷ߒߚ‫ޕ‬ᤘ๺  ᐕ  ᦬  ᣣߦേ‛ታ㛎ᣉ⸳᫟ߩ┫Ꮏ‫ޔ‬ᒁ߈ᷰߒ߇ߥߐࠇ‫ޔ‬หᐕߩ ᦬  ᣣߦⓨ⺞╬ᯏ᪾଻቞▤ℂㆇォߩᬺോᆔ⸤߇㐿ᆎߐࠇ‫  ᦬ ޔ‬ᣣߦ௅ߐࠇߚ⸥ᔨᑼౖ‫ޔ‬⷗ቇળ‫⾐␸ޔ‬ળߦߪ‫↰ޔ‬᎑ཅ㓶ᣣᧄታ㛎േ‛ቇળℂ੐㐳ࠄ߇᜗߆ࠇ‫ޔ‬ጟᧄ㆏ᄦ੩ᄢ✚㐳‫ޔ‬කቇㇱၮ␆⥃ᐥᢎ᝼ࠍᆎ߼ᄙߊߩ㑐ଥ⠪߇㐿ᚲࠍ␸ߞߚ‫ᦨޕ‬ᣂߩᄢⷙᮨߥേ‛ታ㛎ᣉ⸳᫟߳ߩකቇㇱ㑐ଥ⠪ߩᦼᓙߪᭂ߼ߡ㜞߆ߞߚ‫ޕ‬⠉ᐕߩᤘ๺  ᐕ  ᦬  ᣣߦ‫ޔ‬ታ㛎േ‛ߩ៝౉߇ᆎ߹ࠅ‫ޔ‬േ‛ታ㛎ᣉ⸳ߩ೑↪߇㐿ᆎߐࠇߚ‫ޕ‬ᤘ๺  ᐕ  ᦬ߦേ‛ታ㛎ᣉ⸳᫟ߩᱜ㕙ߢ᠟ᓇߐࠇߚࠬ࠲࠶ࡈߩ⸥ᔨ౮⌀ߪ‫ߩ⊝ޔ‬᣿ࠆ޿╉㗻߇ශ⽎⊛ߢ޽ߞߚ‫ߩߘޔ߇ࠈߎߣޕ‬㧟ࠞ᦬ᓟߩ  ᦬  ᣣߦ‫ޔ‬ળ⸘ᬌᩏቭߩᜰ៰ߦࠃࠆᚻ⋥ߒᎿ੐ࠍⴕ߁ߎߣ߇᳿ቯߐࠇ‫  ᦬ ޔ‬ᣣߦߪ‫ޔ‬෼ኈਛߢ޽ߞߚోߡߩേ‛ࠍേ‛ታ㛎ᣉ⸳᫟߆ࠄ৻ᣤ៝಴ߔࠆߣ޿߁ᄢᄌᖤߒ޿੐
ᘒߦ⋥㕙ߔࠆߎߣߦߥߞߚ‫ޕ‬Ꮏ੐⚳ੌᓟ‫ޔ‬หᐕ  ᦬  ᣣߦേ‛ߩౣ៝౉߇㐿ᆎߐࠇߚ‫ޕ‬Ꮏ੐ᦼ㑆ਛߪ‫ᧄޔ‬᫟ߩධ஥㚢ゞ႐ߦࡊ࡟ࡂࡉ⥢ࠍᑪߡ‫ߩࡈ࠶࠲ࠬޔ‬ዬቶߣ৻ㇱߩേ‛ߩ෼ኈቶߦలߡߚ‫ᦼߩߎޕ‬㑆ࠍ೑↪ߒߡ‫᧲ߪ⑳ޔ‬੩ᄢቇක⑼ቇ⎇ⓥᚲߩ₞කቇ⎇ⓥㇱߦࡑ࠙ࠬ࡮
࡜࠶࠻ߩᓸ↢‛ࡕ࠾࠲࡝ࡦࠣᴺߩ⎇ୃߦᵷ㆜ߐࠇ‫ߩ∛࡜࠮࡞ࡉ࠿ࠗޔߦࠄߐޔ‬ᬌᩏᴺߦߟ
޿ߡ߽ቇ߱ᯏળࠍᓧߚ‫ޕ‬㒝ዻേ‛ታ㛎ᣉ⸳ߩᬌ∉࡮ᬌᩏࠪࠬ࠹ࡓࠍ᭴▽ߔࠆߎߣ߇‫ޔ‬₞කᏧߣߒߡߩ⑳ߩᦨೋߩછോߢ޽ߞߚߩߢ‫ޔ‬਄⸥ߩ⎇ୃᯏળ߇ਈ߃ࠄࠇߚߎߣߪਇᐘਛߩᐘ
޿ߢߪ޽ߞߚ‫ޔߒ߆ߒޕ‬කቇ⎇ⓥ⑼ߩవ↢ᣇߩᦼᓙߦᔕ߃ߡ㐿⸳ߒߚㅜ┵ߩᄢⷙᮨߥᚻ⋥ߒᎿ੐ߪ‫ߩ⊝ޔ‬ᗧ᳇ࠍᶖᴉߐߖࠆ߽ߩߢ޽ߞߚ‫ߥ߁ࠃߩߎޕ‬಴᧪੐ߩ⚿ᨐ‫ޔ‬㒝ዻേ‛ታ㛎ᣉ⸳ߩㆇ༡߇ᧄᩰ⊛ߦ㐿ᆎߐࠇߚߩߪ‫ޔ‬ᤘ๺  ᐕᐲ߆ࠄߣ⸒ߞߡ⦟޿ߢ޽ࠈ߁‫ޕ‬േ‛ታ㛎ᣉ⸳ߩᦨೋߩࠬ࠲࠶ࡈߪ‫ޔ‬ഥᢎ᝼‫ޔ‬ഥᚻߦട߃ߡ‫ޔ‬೨ㅀߩ⚐♽േ‛㘺⢒ቶ߆ࠄߩᛛቭߣ㕖Ᏹൕ⡯ຬ‫ޔ‬ᄖ⑼♽ᢎቶߩേ‛ᜂᒰߩᛛቭ‫ޔ‬ᣂⷙណ↪ߐࠇߚേ‛ߩ㘺⢒߿ᵞᵺᜂᒰߩ㕖Ᏹൕ⡯ຬ‫߮ࠃ߅ޔ‬ᬺോᆔ⸤ߒߚⓨ⺞⸳஻▤ℂຬ‫⮮ޔ‬ේ⽷࿅߆ࠄߩᡰេߢ㓹↪ߐࠇߚฃઃ੐ോຬ
ࠄዋᢙߢ᭴ᚑߐࠇߚ‫ޕ‬㧞㧕ታ㛎േ‛ߩᬌ∉࡮ᬌᩏࠪࠬ࠹ࡓߩ᭴▽‫ޔ‬ᗵᨴ∝ߩ⊒↢߅ࠃ߮ߘߩኻ╷৻ߟߩേ‛ታ㛎ᣉ⸳᫟ߢ‫ޔ‬ᄙ⒳㘃ߩᓸ↢‛⛔ᓮ࡟ࡌ࡞߇⇣ߥࠆታ㛎↪േ‛ࠍ౒ߦ㘺⢒ታ㛎ߔࠆ‫ߩߘޕ‬㓙‫ޔ‬ᗵᨴ∝ߩ࠻࡜ࡉ࡞߇ߥ޿⁁ᘒߢേ‛ታ㛎ࠍᕡᏱ⊛ߦⴕ߁ߎߣࠍ‫ޔ‬ዋߥ޿ࠬ࠲࠶ࡈߦࠃߞߡታᣉߢ߈ࠆࠃ߁ߦߔࠆߚ߼‫ޔ‬േ‛⒳ߏߣߦએਅߩࠃ߁ߥᣇ㊎ࠍ␜ߒߚ‫ޕ‬࡮ࡑ࠙ࠬ‫࠻࠶࡜ޔ‬㧦ቯᦼࠦࡠ࠾࡯ᬌᩏ߇ߐࠇߡ޿ࠆᏒ⽼ߩ 52( േ‛ࠍᬌ∉ߥߒߦ៝౉
࡮࠙ࠨࠡ‫ߩઁߩߘޔ࠻࠶ࡕ࡞ࡕޔ‬ዊേ‛㧦ࠦࡦࡌࡦ࡚ࠪ࠽࡞േ‛ࠍ⡊∈⊈‫ޔ‬㥦᳝‫ޔ‬๭ๆེ∔ᖚߥߤߩ⥃ᐥᬌᩏߢㆬ೎ߒߡ៝౉
࡮ࠗ࠿㧦଻ஜᚲ↱᧪ߩࠗ࠿ࠍ⮎ᶎ៝౉ᓟ‫ߩ⩶࡜࠮࡞ࡉߣࠕ࡝࡜ࠖࡈࡠࠢࡒޔ‬ᬌᩏߢㆬ೎ߒ
ߡ૶↪࡮ࡀࠦ㧦଻ஜᚲ↱᧪ߩࡀࠦࠍ‫⥃ޔ‬ᐥᬌᩏߢㆬ೎ߒߡ૶↪࡮ࠨ࡞㘃㧦ᬌ∉ᷣߺߩ߽ߩߦ㒢ቯߒߡ៝౉
ᒰೋ‫߽ᦨޔ‬ജࠍ౉ࠇߑࠆࠍᓧߥ߆ߞߚߩߪ‫ޔ‬ਥߦᄖ⑼♽ߩ⎇ⓥߦᄙ↪ߐࠇߚࠗ࠿ߩ⏕଻ߢ޽ࠆ‫଻ޕ‬ஜᚲ↱᧪ߩࠗ࠿߇૶↪ߐࠇߡ޿ߚ㗃ߢ‫ޔ‬੩ㇺᏒߣ੩ㇺᐭ߆ࠄߩߺߢߪਇ⿷ߣ޿
߁ߎߣߢ‫ޔ‬ṑ⾐⋵‫ޔ‬ጘ㒂⋵‫ޔ‬ᓟߦߪ౓ᐶ⋵ߦ↱᧪ߔࠆࠗ࠿ࠍ㓸߼ߚ‫ޕ‬ᤘ๺  ᐕᐲߩ⸥㍳ߦࠃࠆߣ‫ޔ‬Ფ᦬ᐔဋ  㗡‫ޔ‬ᐕ㑆  㗡ࠍ૶↪ߒߡ޿ߚ‫ޕ‬ㅏ੢߿ຏ்੐᡿߇ߥ޿ࠃ߁ߦ㚂ベࠍ⏕ታߦઃߌઍ߃‫⮎ޔ‬ᶎ‫ޔ‬ណⴊߦࠃࠆࠗ࠿ᔃ⤳♻⁁⯻ߩࡒࠢࡠࡈࠖ࡜࡝ࠕᬌᩏ‫߮ࠃ߅ޔ‬ࠗ࠿ࡉ࡞࠮࡜∛ߩᬌᩏࠍⴕߞߚ‫⃻ޕ‬࿷ߪ‫଻ޔ‬ஜᚲ↱᧪ߩࠗ࠿ߪ৻ಾ૶↪ߖߕ‫ޔ‬ታ㛎↪ߦ↢↥ߐࠇߚࠗ࠿ߦ㒢ቯߐࠇߡ޿ࠆ‫଻ޕ‬ஜᚲ↱᧪ߩࠗ࠿ߦߪ‫ޔ‬ᬌᩏኻ⽎ߣߒߡ޿ߚᗵᨴ∝ߥߤߦ߽⟕ᖚߒߡ޿ࠆ଀߇ᄙߊ‫ޔ‬ታ㛎↪ߣߒߡ೑↪ߔࠆ਄ߢᦸ߹ߒ߽ߩߢߪߥ߆ߞߚ‫ޕ‬ᓥ੐ߔࠆࠬ࠲࠶ࡈ߿⎇ⓥ⠪ߩ቟ో⏕଻ߣᬌ∉ᬌᩏ޽ࠆ޿ߪᴦ≮ߦⷐߔࠆ⽶ᜂࠍ⠨߃ࠆߣ‫↪⾌ޔ‬ኻലᨐߪᭂ߼ߡૐ޿ߣ⹏ଔߖߑࠆࠍᓧߥ޿‫ᦨޕ‬ㄭߢߪ‫ߩ࠿ࠗޔ‬೑↪ᢙߪỗᷫߒߡ‫ޔ‬ታ㛎↪ߩࠗ࠿߆ࠄታ㛎↪ዊဳࡉ࠲ߦ⒖ⴕߒߡ޿ࠆ‫ޕ‬㒝ዻേ‛ታ㛎ᣉ⸳ߩ㐿⸳ᒰೋ‫࠻࠶࡜ߣࠬ࠙ࡑޔ‬ߪ 52( േ‛ߦ㒢ቯߒࠃ߁ߣឭ᩺ߒߚ߇‫ޔ‬ᒰᤨߩ⃻⁁ࠍ⠨ᘦߒߡ⦟⾰ߩࠦࡦࡌࡦ࡚ࠪ࠽࡞േ
‛ߪࠢ࡝࡯ࡦࠦࡦࡌࡦ࡚ࠪ࠽࡞േ‛ߣ⒓ߒߡ‫ޔ‬52( േ‛ߩ㘺⢒㗔ၞߣߪ㗔ၞࠍಽߌߡ೑↪น⢻ߣߒߚ‫ޕ‬㘺⢒ⵝ⟎ߦߟ޿ߡߪ‫ߩ⵾࠻ࠗࡑ࡞ࠕߪࠫ࡯ࠤࠬ࠙ࡑޔ‬㕦▫࠲ࠗࡊ‫ࠤߪ࠻࠶࡜ޔ‬࡯ࠫߩࡔ࠶ࠪࡘᐥ߆ࠄ⪭ߜࠆᳪ‛ࠍ⥄⿛ᑼߩࡌ࡞࠻ࠦࡦࡌࠕߢ៝಴ߔࠆ઀᭽ߩ⥄േ㘺⢒ⵝ⟎ߣ๭߫ࠇߚ߽ߩ߇ណ↪ߐࠇߚ‫ࠬ࡞ࠗ࠙ࠗ࠳ࡦ࠮ޔߪߢࠬ࠙ࡑޕ‬ኻ╷߇ᒰೋߩᦨ߽ᄢ߈ߥ
⺖㗴ߢ޽ߞߚ㧔ᢥ₂㧝㧕‫ߩߘޕ‬ᓟ‫ޔ‬ᜂ≸⹜㛎ߥߤߦᄙᢙߩ࠿࡯࠼ࡑ࠙ࠬ߇೑↪ߐࠇࠆࠃ߁ߦ
ߥߞߚ㗃ߦߪ‫ࠬ࠙ࡑޔ‬⢄Ἳ࠙ࠗ࡞ࠬ߿࠾ࡘ࡯ࡕࠪࠬ࠴ࠬࠞ࡝࠾ᳪᨴߣߘߩኻ╷߇ᣂߚߥ⺖㗴ߣߥߞߚ㧔ᢥ₂㧞㧕‫ޕ‬ᳪᨴࠍ⚻㛎ߔࠆᐲߦ‫ࠍࠫ࡯ࠤޔ‬୘೎ൻߔࠆߚ߼ߩࡈࠖ࡞࠲࡯࠻࠶ࡊ
ߩ೑↪‫ޔ‬㘺⢒㗔ၞߩಽഀ‫ޔ‬Ṍ⩶⌕⴩ߩ੤឵࿁ᢙߩ⷗⋥ߒ‫ޔ‬ᓸ↢‛ࡕ࠾࠲࡝ࡦࠣߩᒝൻߥߤ
ࠍⴕߞߚ‫ ߩ࠻࠶࡜ߣࠬ࠙ࡑޕ‬52( ࠦࡠ࠾࡯ࠍᓸ↢‛ᳪᨴ߆ࠄ቞ࠆߦߪ‫ߩ࠻ࡈ࠰ߣ࠼࡯ࡂޔ‬ਔ㕙ࠍ෩ᩰߦᢛ஻ߒߥߌࠇ߫ߥࠄߥ޿‫ޔࠄ߇ߥߒ߆ߒޕ‬ታ㓙ߦߪ‫߆߅ߩ࡯ࠩ࡯࡙ߣ▚੍ޔ‬ࠇߡ޿ࠆ⁁ᴫߦᏀฝߐࠇࠆߣߎࠈ߇ᄙߊ‫⺞ࠍࠄࠇߘޔ‬ᢛߒߡㅴ߼ߑࠆࠍᓧߥ߆ߞߚ‫ޕ‬㧟㧕ኾછᢎ᝼ߩ⺀↢ߣࡂࡦ࠲࠙ࠗ࡞ࠬᳪᨴ
⮮ේᣉ⸳㐳ߩㅌቭߦ઻޿‫ޔ‬ᤘ๺  ᐕ߆ࠄ⮎ℂቇᢎቶߩ㜞᛬ୃੑᢎ᝼߇㒝ዻേ‛ታ㛎ᣉ⸳㐳ࠍ૬છߐࠇߚ‫ߏޕ‬ሽߓߩࠃ߁ߦጊ↰ᷕਃഥᢎ᝼ߪ‫ޔ‬㑐⷏࿾඙ߦ߅޿ߡታ㛎േ‛ቇߥࠄ߮ߦ㑐ㅪ⻉⑼ቇߩ⊒㆐ࠍ࿑ࠆ੐ࠍ⋡⊛ߣߒߡ‫ޔ‬ᤘ๺ᐕߦ‫ޟ‬ታ㛎േ‛㓸⺣ળ‫ޠ‬‫ߡߒߘޔ‬ᤘ๺ᐕ᦬ߦ‫ޟ‬㑐⷏ታ㛎േ‛⎇ⓥળ‫ޔࠇߐ┙⸳ࠍޠ‬ೋઍળ㐳ߣߒߡળߩㆇ༡ߣ⊒ዷߦዧജߐࠇߡ޿ߚ‫ޔߒ߆ߒޕ‬ᒰೋ‫ޔ‬࿖┙ᄢቇකቇㇱ㒝ዻേ‛ታ㛎ᣉ⸳ߦኾછߩᢎ᝼ࡐࠬ࠻߇㈩⟎ߐࠇߡ޿ߥ߆ߞߚ‫ޕ‬ᣥ㧣Ꮲᄢࠍవ㗡ߦߒߡ‫ޔߚ߹ޔ‬࿖┙ᄢቇേ‛ታ㛎ᣉ⸳ද⼏ળࠍ⢛᥊ߦߒߡ‫ߩߘޔ‬ᔅⷐᕈࠍ⸷߃ࠆᵴേ߇ᑧ‫ޔߡߒߘޕߚࠇߐߥߣޘ‬ᤘ๺  ᐕ  ᦬ߦጊ↰వ↢߇ᣂߚߦ㈩⟎ߐࠇߚኾછᢎ᝼ߩࡐࠬ࠻ߦዞછߐࠇ‫ޔ‬⠉ᐕߩᤘ๺  ᐕᐲ߆ࠄേ‛ታ㛎ᣉ⸳㐳ࠍ૬છߐࠇߚ‫ߩߎޕ‬㗃‫ౕޔ‬૕⊛ߦߪᤘ๺  ᐕ߆ࠄ  ᐕߩߎߣߢ޽ࠆ߇‫ޔ‬ᚒ߇࿖ߩᄢቇ⎇ⓥᯏ㑐╬ߩേ‛ታ㛎ᣉ⸳ߦ߅޿ߡࡂࡦ࠲࠙ࠗ࡞ࠬߩᳪᨴ߇ᄢ߈ߥ໧㗴ߦߥߞߡ޿ߚ‫ߩߘޕ‬ਛߢ߽‫ᧅޔ‬ᏻක⑼ᄢቇߦ߅޿ߡ‫ޔ‬േ‛㘺⢒ᜂᒰ⠪߇ਇᐘߦ߽ᗵᨴᓟߦ੢ߊߥࠄࠇࠆߣ޿߁࡚ࠪ
࠶ࠠࡦࠣߥ಴᧪੐߇޽ߞߚ‫ࠆߌ߅ߦ࠻࠶࡜ߩߎޕ‬ᓸ↢‛ᳪᨴߪ‫ోޔ‬࿖⊛ߥኻ╷߇⻠ߓࠄࠇߚߎߣߦࠃࠅ৻Ბ⪭ߒߚߣᕁࠊࠇߡ޿ߚ߇‫ޔ‬ᐔᚑ  ᐕ  ᦬ߦ‫ޔ‬੩ㇺᄢቇߢߩࡂࡦ࠲࠙ࠗ࡞
ࠬᳪᨴ߇ᣂ⡞߿࠹࡟ࡆߢᄢ߈ߊႎ㆏ߐࠇߚ‫ޔߪ┵⊒ޕ‬㒝ዻേ‛ታ㛎ᣉ⸳᫟ߣߪ⇣ߥࠆ⥃ᐥ
⎇ⓥ᫟ߢ⛽ᜬߐࠇߡ޿ߚ࡜࠶࠻ߩേ‛ታ㛎ᣉ⸳᫟߳ߩ៝౉ଐ㗬ߢ޽ߞߚ‫ߞⴕߦࡦ࠴࡯࡞ޕ‬ߡ޿ߚᓸ↢‛ᬌᩏߩ⚿ᨐ‫ⶄޔ‬ᢙߩ∛ේ૕߇㓁ᕈߢ޽ߞߚߩߢ‫ޔ‬㒋ᄢᓸ⎇ߦࡂࡦ࠲࠙ࠗ࡞ࠬ
᛫૕ߩㅊടᬌᩏࠍଐ㗬ߒߚߣߎࠈ‫ߡߴߔ߇࠻࠶࡜ߩࠄࠇߘޔ‬㓁ᕈߢ޽ࠆߣ⸻ᢿߐࠇߚ‫ߘޕ‬ࠇࠄߩ࡜࠶࠻߇⛽ᜬߐࠇߡ޿ߚࠦࡠ࠾࡯߆ࠄឭଏࠍฃߌߚ೎ߩᢎቶߩ࡜࠶࠻ߦ߽ᳪᨴ߇⷗
ߟ߆ߞߚߎߣ߆ࠄ‫ޔ‬කቇㇱߩߺߥࠄߕ੩ㇺᄢቇߩోㇱዪߩ࡜࠶࠻ࠍኻ⽎ߦ⺞ᩏࠍታᣉߒߚ‫ޕ‬ߘߩ⚿ᨐ‫ޔ‬ᳪᨴ߇⇼ࠊࠇࠆࠦࡠ࠾࡯߇ⶄᢙ⷗ߟ߆ߞߚ‫ߡߴߔޔߪࠄࠇߘޕ‬㐽㎮ߒߡ෩ᩰߥ
ᶖᲥಣ⟎ࠍ⻠ߓߚ‫ޕ‬ᓥ੐⠪ߩⴊᷡ᛫૕ᬌᩏࠍታᣉߒߚߣߎࠈ‫ޔ‬ᗵᨴࠍ⇼ࠊࠇࠆ଀߇޽ߞߚ
߇‫ޔ‬ᐘ޿ߦ߽⥃ᐥ∝⁁ࠍ␜ߔ߽ߩߢߪߥ߆ߞߚ‫ޕ‬කቇㇱ㒝ዻേ‛ታ㛎ᣉ⸳ߩታ㛎↪࡜࠶࠻ࠦࡠ࠾࡯߽㐽㎮ኻ⽎ߣߒߚߩߢ‫⎇ࠆ޿↪ࠍ࠻࠶࡜ޔ‬ⓥߪో㕙⊛ߦਛᢿߒߚ‫࡞ࠗ࠙࠲ࡦࡂޕ‬ࠬᳪᨴࠍቢోߦᢿߜ‫ߩߘޔ‬ᓟߩ቟ో቟ᔃߥ㘺⢒ታ㛎ⅣႺࠍ⏕଻ߔࠆ਄ߢߪᔅ㗇ߥߎߣߢ޽
ࠆߣ್ᢿߒߚ⚿ᨐߢ޽ࠆ㧔ᢥ₂㧟㧕‫ޕ‬㧠㧕╙ੑ਎ઍࠍᜂ߁ᐔᚑ  ᐕ  ᦬ᧃߦጊ↰ᷕਃᢎ᝼߇ቯᐕㅌቭߐࠇߚᓟ‫ޔ‬േ‛ታ㛎ᣉ⸳㐳ߪ∛ℂቇᢎቶߩᣣ
วᒄᢎ᝼߇ዞછߐࠇߚ‫ߩߘޕ‬ᓟ‫ޔ‬ᐔᚑ  ᐕ  ᦬ߦ⧔Ꮉ߇ᢎ᝼ߦዞછߒ‫ޔ‬ᐔᚑ  ᐕᐲ߆ࠄേ
‛ታ㛎ᣉ⸳㐳ࠍ૬છߒߚ‫ޕ‬หᤨᦼߩᐔᚑ  ᐕ  ᦬ߦർ↰৻ඳഥᚻ߇⿞છߒߚ‫ޕ‬⠉ᐕߩᐔᚑ ᐕ  ᦬  ᣣߦ㒋␹᷆〝ᄢ㔡ἴ߇⊒↢ߒߚ‫ޕ‬੩ㇺߪή੐ߢ޽ߞߚ߇ⵍኂߩᄢ߈߆ߞߚ␹ᚭᄢ
ቇකቇㇱ㒝ዻേ‛ታ㛎ᣉ⸳ߩᢇេᵴേࠍṑ⾐ක⑼ᄢቇߩ㠽ዬ㓉ਃవ↢߆ࠄ๭߮߆ߌࠄࠇ‫ޔ‬ᐔᚑ  ᐕ  ᦬  ᣣ߆ࠄ  ᣣߦ߆ߌߡ‫ޔ‬ᢎ⡯ຬ  ฬࠍᵷ㆜ߒߚ‫ޕ‬േ‛ታ㛎ᣉ⸳ߦ߅ߌࠆ㔡ἴኻ╷ߦߟ޿ߡߪ‫ޔ‬࿾㔡࿖ߩᣣᧄߦ߅޿ߡߪᄢᄌᖠ߹ߒ޿໧㗴ߢ޽ࠆ‫ޕ‬ᐔᚑ  ᐕ  ᦬ ᣣߩ᧲ᣣᧄᄢ㔡ἴߣߘߩᓟߩ㔚ജଏ⛎ਇ⿷߆ࠄ‫⥄ޔ‬ኅ⊒㔚ⵝ⟎ߥߤߩ᜛ల߇කቇ⎇ⓥ⑼ౝ

ߢክ⼏ߐࠇߚ‫ޕ‬ᅤ૗ߦ㕖Ᏹᤨߩࡃ࠶ࠢࠕ࠶ࡊࠍᜬߡࠆ߆߇ᓥ೨߆ࠄߩᄢ߈ߥ࠹࡯ࡑߢ޽ࠆ‫ޕ‬㧡㧕㒝ዻേ‛ታ㛎ᣉ⸳᫟ߩჇ▽ߣᡷୃᐔᚑ  ᐕ  ᦬  ᣣߦ㐿௅ߐࠇߚേ‛ታ㛎ᣉ⸳ߩ㐿⸳  ๟ᐕߩ⸥ᔨ⻠Ṷળߦ߅޿ߡ‫ޔ‬ᤤᄛࡈ࡞࠲ࠗࡓߢⒿ௛ߒߡ߈ߚേ‛ታ㛎ᣉ⸳᫟ߩ⸳஻ߪ‫ߦߢߔޔ‬ᡷୃߩᤨᦼ߇೔᧪ߒߡ޿ࠆ
ߎߣࠍႎ๔ߒߚ‫ߩߘޕ‬ᓟ‫ޔ‬ᐔᚑ  ᐕ  ᦬  ᣣߦർ↰ഥᚻ߇ർᶏ㆏ᄢቇߩഥᢎ᝼ߣߒߡォ಴ߐࠇ‫ޔ‬หᐕ  ᦬  ᣣߦᐶᧄ㜞ᔒ⻠Ꮷ߇࿖┙߇ࠎ࠮ࡦ࠲࡯⎇ⓥᚲ߆ࠄ⿞છ‫ ޔ‬ᐕᓟߩᐔᚑ ᐕ  ᦬  ᣣߦߪಎᢎ᝼ߦዞછߒߚ‫ޕ‬㐿⸳  ๟ᐕ߆ࠄ  ᐕࠍ⚻ߡ‫  ⚂ޔ‬ᐕ㑆೑↪ߒߚ㒝ዻേ‛ታ㛎ᣉ⸳᫟ߩ⠴㔡Ꮏ੐‫߮ࠃ߅ޔ‬᜛ᒛᎿ੐ߩ⸘↹߇ᐔᚑ  ᐕᐲ߆ࠄᆎ߹ࠅ‫ޔ‬ᐔᚑ  ᐕᐲߦᣉⴕߐࠇߚ‫ޕ‬ೋᦼߩേ‛ታ㛎᫟ߪ‫ޔ‬࿾ਅ  㓏‫ޔ‬࿾਄㧠㓏ߢ޽ࠅ‫ޔ‬࿾ਅߪ࿾਄㓏ࠃࠅ߽᧲஥ߦᐢ޿ࠬࡍ࡯ࠬ߇޽ࠅ‫ߩߘޔ‬਄ߦ࿾਄㓏ࠍㅊടߔࠆߎߣ߇น⢻ߥࠃ߁ߦ⸳⸘ߐࠇߡ޿
ߚ‫ޔߦߎߘޕ‬㋕㛽⵾ߢ࿾਄  㓏ߩჇ▽ࠍߒߚߩߢ޽ࠆ‫ޕ‬ᡷୃߦ޽ߚߞߡߪ‫ޔ‬એਅߩᣇ㊎ࠍ␜ߒߚ‫ޕ‬㧔㧝㧕ᐢߊ  ࡮කቇ⎇ⓥ⑼ߩേ‛㘺⢒ታ㛎ࠬࡍ࡯ࠬߩ⏕଻㧔㧞㧕⟤ߒߊ  ࡮ౝ߽ᄖ߽ઁߩᑪ‛ࠃࠅ߽⟤ߒߊⴡ↢⊛ߦ  ࡮቟ోᕈߩ⏕଻㧔ᓥ੐⠪ߣⅣႺ߳ߩ㈩ᘦ㧕  ࡮⥇޿ߣേ‛ࠕ࡟࡞ࠥࡦߩ㒰෰‫ޔ‬シᷫ㧔㧟㧕૶޿߿ߔߊ  ࡮ᯏ⢻ᕈ㧔േ✢ߩන⚐ᕈ㧕  ࡮㒐‽ኻ╷  ࡮⎇ⓥ⛮⛯߳ߩ㈩ᘦ  ࡮⚻⾌▵⚂㧔ૐ޿࡜ࡦ࠾ࡦࠣࠦࠬ࠻㧕㧔㧠㧕ᑪ▽ၮḰ㗅቞ᣢ⸳ㇱಽߣჇ▽ㇱಽࠍ৻૕ൻߒߡ‫↪ޔ‬ㅜߦᔕߓߚᮨ᭽ᦧ߃ࠍⴕ޿‫ޔ‬⠧᧎ൻߒߚᑪ‛ߩᄢⷙᮨߥჇᡷ▽ࠍⴕߞߚ‫ޕ‬ᗵᨴታ㛎㗔ၞߩ⸳⟎‫ޔ‬ᵞᵺṌ⩶ቶߩ⒖ォᢛ஻‫࠻࠶࡜ߣࠬ࠙ࡑޔ‬㘺⢒㗔ၞߩ૞ᬺ⠪஥߆ࠄേ‛㘺⢒᫜߳ߩ৻ᣇะ᳇ᵹࠪࠬ࠹ࡓߩዉ౉߇ਥߥᡷༀὐߢ޽ߞߚ‫ޕ‬ో㙚ߦ߅ࠃ߱ᡷୃᎿ੐ߣჇ▽Ꮏ੐ࠍ⚂  ᐕ㑆ߢ⚳߃ࠆᔅⷐ߇޽ߞߚߩߢ‫ޔ‬෼ኈߐࠇߡ޿ߚ
േ‛ߣࠬ࠲࠶ࡈߪߔߴߡേ‛ታ㛎ᣉ⸳᫟ᄖߦ಴ߐࠇ‫ޔ‬ቇౝᄖߦ৻ᤨ⊛ߥ㘺⢒ታ㛎ᣉ⸳߿઒ߩዬቶࠍᓧߚ‫ᦼߩߎޕ‬㑆‫ᦨޔ‬ዊ㒢ߩ㘺⢒ታ㛎ࠍⴕ߁ߎߣ߇ߢ߈ࠆࠃ߁ߦߣ᭽‫ߥޘ‬Ḱ஻ࠍߒߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬ᚲ⹢޿ߕࠇ߽චಽߥ߽ߩߢߪߥߊ‫ޔߪߦࡈ࠶࠲ࠬߣ࡯ࠩ࡯࡙ޔ‬ᄢᄌߥߏ⧰ഭࠍ߅߆ߌߔࠆߎߣߦߥߞߚ‫ޕ‬ℂᗐ⊛ߦߪ೎ߩ႐ᚲߦᣂ▽ߒߚᓟߦേ‛╬ࠍ⒖േߐߖ‫ޔ‬ߘߩᓟߦᣢሽߩᑪ‛ࠍ⸃૕ߔࠆߎߣ߇⦟޿ߩߢߪ޽ࠆ߇‫߇ࠇߘޔ‬ฟ߃ࠄࠇߥ߆ߞߚߎߣߪ

ᱷᔨߢ޽ߞߚ‫ޕ‬ᑪ‛ߩᑧᐥ㕙Ⓧߪ‫ ޔ‬ᐔᣇࡔ࡯࠻࡞߆ࠄ  ᐔᣇࡔ࡯࠻࡞‫  ⚂ޔ‬୚ᄢ߈ߊߥࠅ‫ޔ‬േ‛ߩ෼ኈน⢻ᢙ߽Ⴧᄢߒߚ‫ޔߡߒߘޕ‬Ⴧ▽ᡷୃߐࠇߚᣂേ‛ታ㛎ᣉ⸳᫟ࠍᐔᚑ  ᐕ  ᦬  ᣣ߆ࠄᧄᩰ⊛ߦ೑↪ߔࠆߦ޽ߚߞߡ‫ޔ‬කቇㇱࠠࡖࡦࡄࠬౝߩࡑ࠙ࠬߪ 52(േ‛ߦ㒢ቯߔࠆߎߣ߿‫ޔ‬㘺⢒▤ℂ⚻⾌ࠍ৻ᓞߦ  ୚ߦ୯਄ߍߒߡ‫ޔ‬቟ቯߒߚㆇ༡ࠍⴕ߁
ߚ߼ߩၮ⋚ࠍ⏕଻ߒߚ‫ޔߡߒߘޕ‬ᐔᚑ  ᐕ  ᦬߆ࠄหࠠࡖࡦࡄࠬౝߩ # ᫟࿾ਅ 52( ࡑ࠙ࠬ㗔ၞࠍ㒝ዻേ‛ታ㛎ᣉ⸳ߩ▤ℂਅߦ⒖ⴕߒߡ‫ޔ‬ᐔᚑ  ᐕ  ᦬߆ࠄ⠉ᐕߩᐔᚑ  ᐕ  ᦬߹ߢᡷୃᎿ੐ࠍⴕߞߚ‫ޕ‬ห᭽ߦ‫ (ޔ‬᫟  㓏ߦ⸳ߌࠄࠇߡ޿ߚ 52( ࡑ࠙ࠬ㘺⢒ታ㛎ᣉ⸳ߦߟ޿ߡߪ‫ޔ‬ᐔᚑ  ᐕᐲ߆ࠄห᭽ߦ㒝ዻേ‛ታ㛎ᣉ⸳ߩ৻૕▤ℂਅߦ⟎޿ߡᡷୃᢛ஻Ꮏ੐ࠍⴕߞߚ‫ޕ‬㧢㧕੩ㇺᄢቇേ‛ታ㛎ᆔຬળߣකቇㇱߦ߅ߌࠆേ‛ታ㛎ⅣႺߩᢛ஻᜛లᐔᚑ  ᐕ  ᦬߆ࠄߪ‫ޔ‬‫ޟ‬੩ㇺᄢቇߦ߅ߌࠆേ‛ታ㛎ߩታᣉߦ㑐ߔࠆⷙ⒟‫ߦޠ‬ၮߠߊ‫ޔ‬േ‛ታ㛎ߦ㑐ߔࠆᢎ⢒⸠✵ߩታᣉ‫ޔ‬ታ㛎േ‛ߩ㘺㙃଻▤ᣉ⸳ߣേ‛ታ㛎ቶߩᛚ⹺‫߮ࠃ߅ޔ‬േ‛ታ㛎⸘↹ᦠߩክᩏ߇‫ޔ‬කቇ⎇ⓥ⑼࡮කቇㇱേ‛ታ㛎ᆔຬળߢⴕࠊࠇࠆࠃ߁ߦߥࠅ‫ᧄޔ‬ㇱዪߦዻߔࠆ⎇ⓥ⠪ߩߔߴߡߩേ‛ታ㛎ߪ੩ㇺᄢቇേ‛ታ㛎ᆔຬળߩਅߢⴕࠊࠇࠆ૕೙ߣߥ
ߞߚ‫ߩࠬ࠙ࡑޕ‬෼ኈࠬࡍ࡯ࠬߦߟ޿ߡߪ‫ޔ‬㧲᫟  㓏ߩ 52( ࡑ࠙ࠬ㗔ၞߣߒߡߩ೑↪ࠍ⚳߃ߚߎߣߦ߽㑐ㅪߒߡ‫ࠆߔ଻⏕ߦࠄߐޔ‬ᔅⷐ߇↢ߓߡ‫ޔ‬# ᫟ߩ࿾ਅ 52( ࡑ࠙ࠬ㘺⢒㗔ၞߣേ‛ታ㛎ᣉ⸳᫟ߩ࿾ਅ  㓏ࠍᢛ஻ߒߚ‫ޔߕ߹ޕ‬ᐔᚑ  ᐕ  ᦬߆ࠄ  ᦬ߩᦼ㑆‫ߡߊ⁜ޔ‬૞ᬺᕈߩᖡ߆ߞߚ # ᫟ᵞᵺṌ⩶ቶߩᢛ஻ࠍⴕߞߚ߇‫ޔ‬ⓨ⺞⸳஻ߪᣥᑼߩ߹߹ߢ޽ࠅᦝᣂߩᔅⷐᕈ
߇✕ᕆ⺖㗴ߣߒߡᱷߐࠇߡ޿ࠆ‫ޕ‬ᰴߦ‫ޔ‬ᐔᚑ  ᐕ  ᦬߆ࠄ  ᦬ߩᦼ㑆‫ޔ‬േ‛ታ㛎ᣉ⸳᫟ߩ࿾ਅ  㓏㗔ၞߦ‫ޔ‬ኾ↪ߩ౉ㅌቶ⚻〝ࠍ⸳ߌߡઁߣߪቢోߦ඙ಽߒߡ‫ޔ‬୘೎㓒㔌㘺⢒ⵝ⟎ࠍ஻߃ߚ 52( ࡑ࠙ࠬߩ㘺⢒ታ㛎㗔ၞࠍ⸳⟎ߔࠆᡷୃᎿ੐ࠍⴕߞߚ‫ޔ࠲ࡉޔ࠿ࠗޔߡߒߘޕ‬࠙ࠨࠡߩ㘺⢒ቶࠍᄢ߈ߊ࿐޿ߎߺ‫࠻࠶࡜ࡦ࡯࡝ࠢޔ‬㘺⢒ቶ‫ࠬ࠙ࡑࡦ࡯࡝ࠢޔ‬㘺⢒ቶ‫ޔ‬ᓸ↢‛ࠢ࡝࡯࠾ࡦࠣߩߚ߼ߩࡑ࠙ࠬᓙᯏቶࠍ‫ࠇߙࠇߘޔ‬ᢛ஻㈩⟎ߒߚ‫ ࠅࠃߦࠇߎޕ‬52( ࡑ࠙ࠬߩ෼ኈᢙߪჇ߃ࠆߎߣߦߥߞߚߩߢ‫ޔ‬ᵞᵺṌ⩶ቶߦ㧠ၮ⋡ߩᄢဳߩਔᚺᑼ㜞࿶⫳᳇Ṍ⩶ⵝ⟎ࠍ⸳⟎ߒߡኻಣߔࠆߎߣߦߒߚ‫ޕ‬ᐔᚑ  ᐕ  ᦬ߦߪ‫ޔ‬ᣂേ‛ታ㛎᫟ࠍ೑↪ߒߡ߆ࠄ  ᐕ⋡ࠍㄫ߃ࠆߎߣߦߥࠆ߇‫ޔ‬ታ㛎േ‛ࠍ↪޿ࠆ⎇ⓥߩᔅⷐᕈ߇Ⴧߒߡ߅ࠅ‫ࠆߥࠄߐޔ‬㘺⢒ታ㛎ࠬࡍ࡯ࠬߩ᜛ᄢ߇᳞߼ࠄࠇߡ
޿ࠆ‫ޕ‬േ‛ታ㛎ᣉ⸳᫟ߩᵞᵺṌ⩶㗔ၞߩࠬࡍ࡯ࠬߣᯏ⢻ࠍ⠨ᘦߔࠆߣ‫⁁⃻ޔ‬એ਄ߦዊേ‛ࠍ㘺⢒ߔࠆߎߣߪ࿎㔍ߢ޽ࠆࠃ߁ߦᕁࠊࠇࠆ‫ޕ‬ᔅⷐߦᔕߓߡ‫ޔ‬೎᫟ߩᑪ⸳߇ᬌ⸛ߐࠇࠆߩ
ߢ޽ࠈ߁‫ޕ‬㧣㧕േ‛ታ㛎ᣉ⸳ߩ▤ℂᬺോࠍᝄࠅ㄰ߞߡ
㒝ዻേ‛ታ㛎ᣉ⸳ߩㆇ༡ߦ߅޿ߡߪ‫ޔ‬㑐ଥ⠪ߦᄢᄌߏදജࠍ㗂޿ߚ‫ޔߡߒߘޕ‬ᄢ߈ߥ಴᧪੐߇↢ߓߚ▵⋡‫ޔߪߢ⋡▵ޔ‬ᱧઍߩකቇ⎇ⓥ⑼㐳ߣേ‛ታ㛎ᣉ⸳㐳ߦปງỗബࠍ㗂߈‫ޔ‬߹ߚ‫ߏޔ‬ᡰេࠍ㗂޿ߚ‫ޕ‬േ‛ታ㛎ᣉ⸳ߩㆇ༡ߪᤤᄛࠍ໧ࠊߕભᕷࠍਈ߃ߡߊࠇࠆ߽ߩߢߪ
ߥ޿‫ޕ‬ᢎ⡯ຬ‫ޔ‬㕖Ᏹൕ⡯ຬ‫ޔ‬ᵷ㆜⡯ຬ‫ޔ‬ᬺോᆔ⸤ߩࠬ࠲࠶ࡈߦߪ‫ޔ‬₂り⊛ߦߘࠇߙࠇߩ⡯ോࠍᜂᒰߒߡ㗂޿ߚ‫ߩߎޕ‬ᡰ߃ߥߒߦ‫ޔ‬Ꮒᄢߥേ‛ታ㛎ᣉ⸳ࠍㆇ༡ߔࠆߎߣ߇ߢ߈ߥ߆ߞ
ߚ‫ޕ‬ᷓߊᗵ⻢↳ߒ਄ߍࠆ‫ߥ⊛⛯⛮ߪߦࡈ࠶࠲ࠬޕ‬ദജࠍᒁ߈⛯߈᳞߼ߑࠆࠍᓧߥ޿߇‫ߘޔ‬ࠇߣ౒ߦ‫ޔ‬ᅤ૗ߦ஻߃ࠍలታߐߖߡ߅ߊ߆߇ᭂ߼ߡ㊀ⷐߢ޽ࠆ‫⽶↪⾌ߪߦࠇߘޕ‬ᜂ߇ᄢ߈޿‫ޔߒ߆ߒޕ‬ઃടଔ୯ߩᄢ߈޿േ‛ታ㛎ߢ޽ࠅ‫ߣߔ⋥ࠅ߿ޔ‬ᄙᄢߥࠦࠬ࠻ࠍⷐߔࠆߎߣࠍ
චಽߦ⹺⼂ߒߡ‫್ޔ‬ᢿߔࠆߎߣ߇ᔅⷐߢ޽ࠆ‫ޕ‬කቇ↢‛ቇߩၮ␆⎇ⓥߣᔕ↪⎇ⓥߦ߅޿ߡ‫ޔ‬ታ㛎േ‛ߣേ‛ታ㛎ߪ‫◲߁ߘޔ‬නߦᓎഀࠍ
⚳߃ߘ߁ߢߪߥ޿ߣᕁ߁‫ޕ‬ఝࠇߚ⎇ⓥߩᚑᨐ߇⋉‫ޘ‬ᓧࠄࠇࠆߎߣࠍᔃ߆ࠄ␨ᔨߔࠆ‫ޕ‬㧞㧚੩ㇺᄢቇߢߩ⎇ⓥᵴേ㧝㧕ᗵᨴ∝⎇ⓥ
వߦㅀߴߚࠃ߁ߦ‫ޔ‬ᤘ๺ 50 ᐕߩᄐ߆ࠄ⑺ߦ‫ޔ‬ળ⸘ᬌᩏቭߩᜰ៰ߦࠃࠆേ‛ታ㛎ᣉ⸳᫟ߩᚻ⋥ߒᎿ੐߇ⴕࠊࠇߚ‫ߩߘޕ‬㓙ߦ‫ߩࠬ࠙ࡑޔ‬ᓸ↢‛ࡕ࠾࠲࡝ࡦࠣߦ૶↪ߐࠇᆎ߼ߡ޿ߚELISA ᴺߩ⠌ᓧࠍ⋡⊛ߦ‫᧲ޔ‬੩ᄢቇක⑼ቇ⎇ⓥᚲ㧔ක⑼⎇㧕₞කቇ⎇ⓥㇱߦᵷ㆜ߐࠇߚ‫ޕ‬ߘߩᦼ㑆ਛߦ‫ޔ‬਄↰㓶ᐙవ↢ߣᒰᤨᄢቇ㒮↢ߢ޽ࠅᧄ⎇ⓥળߩળຬߢ޽ࠆਃᨑ㗅ਃߐࠎ߆ࠄ‫ߡ޿ߟߦ∛࡜࠮࡞ࡉ࠿ࠗޔ‬ᢎࠊߞߚ‫ޕ‬ᤤભߺߦߪ‫ޔ‬૒‫ ޘ‬ቇవ↢ߩነ↢⯻ቇ⎇ⓥቶߦ߽㗻ࠍ಴ߒߡ‫ߩⰆ߿࠾࠳ޔ‬㘺⢒ᴺ‫ߩࠕ࡝࡜ࠖࡈ߮ࠃ߅ޔ‬ᗵᨴታ㛎ࠍ⷟߆ߖߡ㗂޿ߚ‫ޕ‬⍴޿ᦼ㑆ߢߪ޽ߞߚ߇‫߇ߡోޔ‬ᣂ㞲ߢᄢᄌߦਛりߩỚ޿ᣣ‫ޕߚߞ޽ߢޘ‬ࠗ࠿ࡉ࡞࠮࡜∛ߩ⎇ⓥߪ‫ޔ‬േ‛ታ㛎ᣉ⸳ߩᬌ∉ᬺോࠍዷ㐿ߒߡታᣉߒߚ߽ߩߢ޽ߞߚ‫ޕ‬వߦㅀߴߚࠃ߁ߦ‫ޔ‬ᒰᤨ‫଻ޔ‬ஜᚲ↱᧪ߩࠗ࠿߇Ფ᦬⚂ 100 㗡೨ᓟ៝౉ߐࠇߡ޿ߚ‫ࡉ࠿ࠗޕ‬࡞࠮࡜⩶ Brucella canis ߩᬌ಴₸ߪ‫ ⚂ޔ‬2.8% (33 / 1,186 㗡‫ޔ‬ጘ㒂⋵ߣṑ⾐⋵↱᧪㧕ߢ޽ߞߚ㧔ᢥ₂㧠㧕
‫ߦ⩶࡜࠮࡞ࡉ࠿ࠗޕ‬ᗵᨴߒߚ㓶›߇᛫♖ሶ⥄Ꮖ఺∉∔ᖚࠍ๒ߔࠆߎߣࠍ‫⥄ޔ‬ὼᗵᨴ›ߣታ㛎ᗵᨴ›ࠍ↪޿ߡ᣿ࠄ߆ߦߒߚ㧔ᢥ₂㧡㧕‫ޕ‬ᢎോ⡯ຬߩ᧛ญᱞᒾߐࠎߦട߃ߡ‫ޔ‬ᄢ㒋ᐭ┙ᄢቇߩห⚖↢ߢ޽ߞߚ↰ㄝ⵾⮎ߩ㜞↰ ඳߐࠎ߇ਥߥ౒ห⎇ⓥ⠪ߢ޽ߞߚ‫ޕ‬ᒰᤨ‫ޔ‬↰ㄝ⵾⮎቟ోᕈ⎇ⓥᚲߩ⊝ߐࠎߪ‫⎇ߩߜߚ⑳ޔ‬ⓥࠍ㓐ಽࠨࡐ࡯࠻ߒߡਅߐߞߚ‫ޕ‬ਇⷐߣߥߞߚࡆ࡯ࠣ࡞›ࠍ⼑ࠅ߁ߌࠆߚ߼ߦ࠻࡜࠶ࠢߢ૗ᐲ߽಴߆ߌߚ‫ޔߪߦᤨޕ‬ណขߒߚಓ⚿⚵❱ࠍᜬෳߒߡ‫ࠆࠃߦ࠻࠶࠲ࠬࠝ࡝ࠢޔ‬ᮡᧄ૞ᚑߣⰯశ㗼ᓸ㏜ߦࠃࠆⷰኤࠍታᣉߐߖߡ߽

ࠄߞߚ‫⎇ޕ‬ⓥ⾌ߪߥߊ‫ޔߕ߈ߢߪߣߎࠆߔ౉⾼ࠍ›࡞ࠣ࡯ࡆޔ‬ᔅⷐߥታ㛎ᯏེࠍᜬߞߡ޿
ߥ߆ߞߚ߆ࠄߢ޽ࠆ‫ߚ޿ߡ᧪ߦୃ⎇ޕ‬ᣣᧄㄘ↥Ꮏᬺᩣᑼળ␠ߩ౉ᳯᵏᶈߐࠎߩ⚫੺ߢ‫ޔ‬ᮮᵿ߹ߢࡆ࡯ࠣ࡞›ࠍ࠻࡜࠶ࠢߢ⾃޿ߦⴕߞߚߎߣ߽޽ߞߚ‫ޕ‬ጤୖߩ㐿ᬺ₞කᏧߢ‫ߏޔ‬ㅤ෰ߐࠇߚਛየ ⊓వ↢߽⧯߆ࠅߒ㗃‫⺞ߩ∛ᧄޔ‬ᩏ⎇ⓥߦෳടߒߡ㗂޿ߚ‫ޕ‬Ꮢ⽼ߐࠇߡ޿ߥ߆ߞ
ߚ FITC ᮡ⼂᛫ࠗ࠿ IgA ࠙ࠨࠡ᛫૕ࠍ‫ޔ‬IgA ߩ♖⵾߆ࠄᚻដߌߡ૞⵾ߒߚ‫࠿ࠗޔߡߒߘޕ‬ࡉ࡞࠮࡜ᗵᨴ›ߩ♖▤߿ዩᴉᩏਛߦ⷗ߟ߆ࠆ㗡ㇱኻ㗡ㇱߩ⥄Ꮖಝ㓸♖ሶߦ෻ᔕߐߖߚ‫ޕ‬ಝ㓸♖ሶߩ㗡ㇱࠕࠢࡠ࠰࡯ࡓ߇Ⱟశࠍ⊒ߒߡ⷗߃ߚᤨߪ‫ᦼޔ‬ᓙߤ߅ࠅߢࡃࡦࠩࠗࠍߒߡ༑ࠎ
ߛ㧔ᢥ₂㧢㧕
‫ⴊޕ‬ᷡਛߩ♖ሶಝ㓸᛫૕ߩജଔࠍ⺞ߴࠆߚ߼ߦߪ‫ޔ‬ᱜᏱߥᣂ㞲♖ሶ߇ᔅⷐߢ޽
ߞߚ‫ޔߢߎߘޕ‬ኈᤃߦ♖ᶧࠍណขߐߖߡߊࠇࠆଢ೑ߥࡆ࡯ࠣ࡞›ࠍ 2 㗡Ḱ஻ߒߡ‫ߢߟ޿ޔ‬߽⎇ⓥߔࠆߎߣ߇ߢ߈ࠆࠃ߁ߦ߽ߒߚ‫ߚ޿↪ࠍ࠿ࠗޔߒ߆ߒޕ‬ᗵᨴታ㛎ࠍᧄᩰ⊛ߦታᣉߢ߈ࠆⅣႺࠍേ‛ታ㛎ᣉ⸳ߦ᳞߼⛯ߌࠆߎߣߦߪήℂ߇޽ߞߚ‫ৼޕ‬ᐲߘߩ㗃‫ߩ∛࡜࠮࡞ࡉޔ‬࿖㓙ቇળ߇ࠕ࡞ࠫࠚ࡝ࠕߩࠕ࡞ࠫࠚߢ㐿߆ࠇࠆߎߣࠍ⍮ࠅ‫ޔ‬ਥߥᚑᨐߢ޽ࠆࡉ࡞࠮࡜ᗵᨴ
›ߩ⥄Ꮖ఺∉ᕈ㓶ᕈਇᅧߦߟ޿ߡ⊒⴫ߔࠆߎߣࠍ඙ಾࠅߦߒߡ‫⎇ᧄޔ‬ⓥߦ৻Ბ⪭ࠍઃߌߚ‫ޕ‬ೋ߼ߡߩᄖ࿖ᣏⴕߢ‫ޔ‬ೋ߼ߡߩ࿖㓙ቇળߩ⊒⴫ߢ޽ߞߚ‫ޕ‬ቇળෳടᓟߪࠨࡂ࡜⍾Ṽߦዋߒ┙ߜነࠅ‫⧷ޔ‬࿖ߣ࠼ࠗ࠷ߩ⎇ⓥᚲࠍ⸰໧ߒߡ‫ߩߘޔ‬ᓟߩ⎇ⓥ࠹࡯ࡑࠍ⷗ߟߌࠃ߁ߣ޿߁
⸘↹ߢ޽ߞߚ‫ޔߪߢࠚࠫ࡞ࠕޔߒ߆ߒޕ‬WHO ߩੱ₞౒ㅢᗵᨴ∝ᜂᒰߩ⮮ୖቁᄦవ↢ߦᚻ෩ߒߊᜰዉߐࠇߚ‫⍾࡜ࡂࠨޕ‬Ṽⴕ߈ߪࠠࡖࡦ࠮࡞ߐߖࠄࠇ‫⎇࡞࡯࠷ࠬࡄߩࠚࠫ࡞ࠕޔ‬ⓥᚲߢ
ߩࡉ࡞࠮࡜∛ળ⼏ߦ߶߷ᒝ೙⊛ߦෳടߐߖࠄࠇߚ‫ࠄ߆⓹ߩࠬࡃޕ‬౮⌀ࠍ᠟ࠆߥ‫ߩઁޔ‬ᄖੱࡔࡦࡃ࡯߇޿ࠆߣߎࠈߢ⥄ಽ㧔⮮ୖవ↢㧕ߦᣣᧄ⺆ߢ⹤ߒ߆ߌࠆߥ‫⷗ޔ‬⍮ࠄߧᄖੱ߆ࠄߩ
㗬߹ࠇߏߣߪ⺧ߌ⽶߁ߥߣ⸒ࠊࠇߚ‫⥄ޕ‬ಽߢߪ᳇ઃ߆ߥ߆ߞߚᐔ๺ࡏࠤࠍปࠄࠇߚ‫⌀ޕ‬೶ߦᜰዉࠍฃߌߚߎߣ‫ޔ‬ᄢᄌ޽ࠅ߇ߚ߆ߞߚ‫ޕ‬⧷࿖ߢߪㄘ᳓⋭ߩࡉ࡞࠮࡜∛⎇ⓥㇱ㐷ߣ MRC Ქᕈㇱ㐷ߩ Michael Festing ඳ჻ߩ⎇ⓥ
ቶ‫ߚߞ޽ߦ࡯ࠔࡈ࡯ࡁࡂߪߢ࠷ࠗ࠼ޔ‬ታ㛎േ‛࠮ࡦ࠲࡯ࠍ⸰ࠇߚ‫੹ޔࠇߙࠇߘޕ‬ᓟߩ⎇ⓥ࠹࡯ࡑߩࡅࡦ࠻ࠍ⷗ߟߌ಴ߔߚ߼ߩ⸰໧ߢ޽ߞߚ‫ޔߪߢ࡯ࠔࡈ࡯ࡁࡂޕ‬Zitter rat ࠍ⼑ߞߡ
߶ߒ޿ߣ↳ߒ಴ߚ‫ޕ‬੩ᄢߢหߓߊ૕߇㔡߃ࠆ࡜࠶࠻ Tremor rat ߇⊒⷗ߐࠇߡ߅ࠅ㧔ᢥ₂㧣㧕‫ޔ‬ਔ⠪ߩᲧセ⎇ⓥࠍᏫ࿖ᓟߦߒߚ޿ߣᕁߞߚ߆ࠄߢ޽ࠆ‫ޕ‬ઍࠊࠅߦ‫ޔ‬Tremor rat ࠍឭଏߒࠃ
߁ߣ⸒߁ߣ‫ޔ‬ᣣᧄߢߪታ㛎↪࡜࠶࠻ߦ߅ߌࠆࡂࡦ࠲࠙ࠗ࡞ࠬ߇໧㗴ߣߥߞߡ޿ࠆࠃ߁ߛ߆
ࠄ‫߽߆ߚߞߛ࡯ࠠ࠶࡜ޔߪߩߚߞߥߦߣߎࠆࠇ߿ߢ⥄⁛ޕߚࠇࠊ⸒ߣ޿ߥࠄⷐޔ‬⍮ࠇߥ޿‫ޕ‬㧞㧕࡜࠶࠻ߩㆮવቇ⎇ⓥ
੩ㇺᄢቇߢߪ‫ޔ‬ጊ↰ᷕਃవ↢߇੩ㇺߦ⿞છߐࠇߚᤨ߆ࠄ࡜࠶࠻ߩㆮવቇ⎇ⓥࠍᆎ߼ࠄࠇ
ߡ޿ߚ‫ޕ‬ㄘቇㇱ⇓↥ቇ⑼ߩቇ↢ߦኻߔࠆත⺰⎇ⓥᜰዉ߽ⴕࠊࠇߡ޿ߚ‫ޕ‬ቇ↢߇߿ߞߡߊࠆ
೨ߪ‫ޔ‬ጊ↰వ↢ߣੑੱߢ‫ޔ‬Jan Klein ⪺ߩ Biology of the Mouse Histocompatibility-2
Complex. (Springer-Verlag, 1975) ࠍᢎ⑼ᦠߦߒߡീᒝળࠍ㐿޿ߡ޿ߚ‫ޕ‬൩⺰‫ߩߘޔ‬ᓟߪ‫ޔ‬వ᢯↸ߩ‫ᧁ߿ޠࡦ࡝ࡊ࠶ࡖ࠴ޟ‬ደ↸ߩ‫ߦ࡯ࠠࠬࠗ࠙ߩߢޠࠕࡏࡦࠨޟ‬ᶐߞߚ‫ߚߞ޿߁ߘޕ‬⢛᥊߽޽ࠅ‫⎇∛࡜࠮࡞ࡉ࠿ࠗޔ‬ⓥߩᰴߩ⎇ⓥ࠹࡯ࡑߣߒߡ‫ޔ‬ታ㛎േ‛ࠍ↪޿ߚㆮવቇ⎇ⓥࠍኒ߆ߦ୥⵬ߣߒߡᬌ⸛ߒߡ޿ߚ‫ޕ‬Ꮻ࿖ᓟ‫ޔ‬ᓙߜὶ߇ࠇߚ Zitter rat ߇࠼ࠗ࠷߆ࠄዯ߈‫ޔ‬Tremor hetero rat ߣߩ੤㈩ታ㛎ߦࠃߞߡᓧߚ F1 ਎ઍߦࡒࡘ࡯࠲ࡦ࠻߇↢ߓߥ߆ߞߚߎߣ߆ࠄ‫ߩࠇߙࠇߘޔ‬ේ࿃ㆮવሶߪ⇣ߥࠆߣផቯߒߚ‫ޔߡߒߘޕ‬ਔ࡜࠶࠻ߩේ࿃ㆮવሶࠍ౒ߦࡎࡕߦᜬߟ࡜࠶࠻ SER㧔⥄ὼ⊒∝ߡࠎ߆ࠎ࡜࠶࠻㧕ߩ⺀↢ߦ⥋ߞߚ㧔ᢥ₂㧤㧕‫ޕ‬࿾ਅ 1 㓏ߩ࡜࠶࠻㘺⢒ቶߢ‫ࡦ࠲࡯ࡘࡒ࡞ࡉ࠳ޔ‬࠻ߣᕁࠊࠇࠆ࡜࠶࠻߇ࠤ࡯ࠫౝߢỗߒ޿ߡࠎ߆ࠎ⊒૞ࠍᰴ‫ߡ⷗ࠍߩߔߎ⿠ޘ‬ᄢᄌ㛳གྷߒߚ‫ޕ‬⺕߆ߦߔߋߦ⍮ࠄߖߚߊߡ‫ޕߚ޿ߡߒ࠭࠙࠭࠙ޔ‬28 ᐕ߽೨ߩߎߣߛ߇‫ޕࠆ޿ߡ߃ⷡߊࠃޔ‬ᒰᤨ‫ޔ‬㒝ዻേ‛ታ㛎ᣉ⸳㐳߇ೋઍߩ⮮ේరౖవ↢߆ࠄ⮎ℂቇᢎቶߩ㜞᛬ୃੑవ↢ߦࡃ࠻ࡦ࠲࠶࠴ߐࠇߡ޿ߚ‫ޔߢߎߘޕ‬㜞᛬వ↢ߦ⋧⺣ߒߡ‫⮎ޔ‬ℂቇᢎቶߩഥᢎ᝼ߛߞߚ╣ ᓕผవ↢‫ޔ‬ᄢቇ㒮↢ߢ޽ߞߚᄢ㊁ⴕᒄߐࠎࠄߣߩ౒ห⎇ⓥ߇ᆎ߹ߞߚ‫╣ޕ‬వ↢ߪᐢፉᄢቇߩᢎ᝼ߦዞ

છߐࠇߚᓟ߽‫ޔ‬SER ߦᄢᄌ⥝๧ࠍᜬߞߡ޿ߚߛ߈‫⮎ޔ‬ℂቇ⊛⸃ᨆߣ᛫ߡࠎ߆ࠎ⮎ߩ⹏ଔ♽ߣߒߡ⏕┙ߔࠆ⎇ⓥࠍ⛮⛯ߒߡ㗂޿ߚ㧔ᢥ₂㧥㧕‫ޕ‬ᄢ੐ߥ SER ߛߣ޿߁ߎߣߢ‫ޔ‬ㅳߩೋ߼ߪ੩ㇺߩߏ⥄ቛ߆ࠄ੩ᄢߦ┙ߜነࠅ‫ޔ‬ᓙߚߖߡ߅޿ߚ࠲ࠢࠪ࡯ߦ SER ࠍਸ਼ߖ੩ㇺ㚞߳‫ߘޔ‬ߒߡᣂᐙ✢ߢᐢፉᄢቇ߹ߢ಴ൕߣ޿߁ߎߣࠍ᥉ㅢߦߐࠇߡ޿ߚ‫ޕ‬ਛ࿖ක⑼ᄢቇߦ SER ࠍࡊ࡟࠯ࡦ࠻ߒߚߎߣࠍᗵ⻢ߐࠇ‫╣ޔ‬వ↢ߣ⑳ߪ౒ߦἀ㓁ߦ᜗߆ࠇቴᐳᢎ᝼ߩ⒓ภઃਈᑼౖߦ
ෳ೉ߔࠆߣ޿߁ᯏળ߽ਈ߃ࠄࠇߚ‫⃻ޕ‬࿷߹ߢ‫⎇ࠎ߆ࠎߡޔ‬ⓥߩᜰዉࠍฃߌߡ޿ࠆߎߣ‫ޔ‬ᄢᄌᗵ⻢ߒߡ޿ࠆ‫ޕ‬SER ߣ޿߁ฬ⒓ߪ‫ޔ‬㜞᛬వ↢‫╣ޔ‬వ↢‫ ߩ⑳ߡߒߘޔ‬3 ฬ߇⋧⺣ߒߡ᳿߼ߚ‫ޕ‬㜞᛬వ↢ߪᅱߥ⇛⺆ߢߪߥ޿ߢ޽ࠈ߁ߥߣ‫ޔ‬ㄉᦠߣ⌮߼ߞߎߢ⺞ߴࠄࠇ OK ߣߥߞߚ‫ޕ‬৻ᔕ‫ޔ‬Spontaneously Epileptic Rat ߩ⇛ߣߒߡ޿ࠆ߇‫ޔ‬Sasa ߣ Serikawa ޽ࠆ޿ߪ Shujiߩ S ߇ട๧ߐࠇߡ޿ߡ‫ޔ‬᳇ߦ౉ߞߡ޿ࠆ‫ޕ‬ᄢ㒋⮎⑼ᄢቇߩᢎ᝼ߣߥࠄࠇߚᄢ㊁వ↢ߣߪ‫ޔ‬੹߽ߡࠎ߆ࠎࡕ࠺࡞࡜࠶࠻⎇ⓥߩ⦟߈ࡄ࡯࠻࠽࡯ߣߒߡ⎇ⓥࠍዷ㐿ߒߡ޿ࠄࠇࠆߎߣߪᄢ
ᄌሜߒ޿‫ޕ‬⑳㆐ߩࠣ࡞࡯ࡊߪ‫ޔߗߥޔ‬SER ߇ߡࠎ߆ࠎ᭽ߩ⊒૞∝⁁ࠍ⿠ߎߔߩ߆ࠍ⍮ࠅߚߊߡ‫ޔ‬ේ࿃ㆮવሶߩหቯࠍ⋡ᜰߒߚ‫ޔߒ߆ߒޕ‬SER ⺀↢ᒰᤨ‫ߩ࠻࠶࡜ޔ‬ㆮવᄙဳࡑ࡯ࠞ࡯ߪᲫ⦡ㆮ
વሶ‫↢ޔ‬ൻቇㆮવሶ‫∉఺ޔ‬㑐ଥㆮવሶߦ㒢ࠄࠇߡ߅ࠅ‫ߪࠣࡦ࠾࡯ࡠࠢ࡞࠽࡚ࠪࠫࡐޔ‬ή⻎ߥ⹜ߺߢ޽ߞߚ‫ޔߢߎߘޕ‬ጊ↰వ↢ߩ⎇ⓥ࠹࡯ࡑ‫ߩ࠻࠶࡜ޔ‬ㆮવࡑ࡯ࠞ࡯ߩ㐿⊒‫࠻࠶࡜ޔ‬ㆮવሶ࿾࿑ߩ᭴▽⎇ⓥߦෳടߒߚ‫⎇ޕ‬ⓥ↢ߩੑ㓏ၴᶈሶߐࠎ‫ޔ‬ㄭ⮮ 㕏ߐࠎ‫ޔ‬ự↰ୃ৻ߐࠎ‫ޔ‬⇓↥ቇ⑼ߩቇㇱቇ↢߅ࠃ߮ୃ჻ߩቇ↢߇ߘߩࡊࡠࠫࠚࠢ࠻ࠍᜂᒰߐࠇߡ޿ߚ‫ߩߘޕ‬ᓟ‫ޔ‬᫪᡽ਯߐࠎ߇කቇ⎇ⓥ⑼ߩඳ჻⺖⒟ߦㅴ߹ࠇࠆߎߣߦߥߞߚ‫ޕ‬ጊ↰వ↢ߪ᫪ߐࠎߦࠨࠩࡦࡂ
ࠗࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦߦࠃࠆ RFLP㧔Restriction Fragment Length Polymorphism‫ޔ‬೙㒢㉂⚛ᢿ㐳ᄙဳ㧕ߩ♽ࠍ࡜ࡏߢ┙ߜ਄ߍࠆࠃ߁ᜰ␜ߐࠇߚ‫ߡ޿߅ߦ࠻࠶࡜ޔߡߒߘޕ‬ㆮવሶߦ⋥⚿ߔࠆ DNA ࡑ࡯ࠞ࡯߇ขࠅᛒ߃ࠆࠃ߁ߦߥߞߚ㧔ᢥ₂ 10㧕‫ޕ‬ࡈ࡜ࡦࠬߩࡄࠬ࠷࡯࡞⎇ⓥᚲߩູ੃േ‛ቇㇱ㐷߳ߩ⇐ቇ߇‫⎇ޔ‬ⓥߩᄢ߈ߥዷ㐿ࠍ߽ߚࠄߒߚ‫ޕ‬ㇱ㐷㐳ߩ Jean-Louis Guénet వ↢ߪ‫ޔ‬₞කᏧߢ޽ࠅࡑ࠙ࠬߩㆮવቇ⠪ߢ޽ߞߚ‫ޕ‬
Guénet వ↢ߪ‫⸃ࠍ࡯ࠞ࡯ࡑ࠻ࠗ࡜࠹ࠨࡠࠢࠗࡑߩࠬ࠙ࡑޔ‬ᨆߔࠆߚ߼ߩ PCR ࡊ࡜ࠗࡑ࡯
ࠍ࡜࠶࠻ߦㆡ↪ߔࠆߣ޿߁ࠕࠗ࠺ࠕߢ‫⋧ࠆߌ߅ߦ࠻࠶࡜ޔ‬หߥ㗔ၞߩㆮવࡑ࡯ࠞ࡯ߩ㐿⊒ߣᲧセࠥࡁࡓࡑ࠶ࡇࡦࠣࠍ⋡ᜰߔߎߣࠍឭ᩺ߐࠇߚ‫ࠗ࡜࠹ࠨࡠࠢࠗࡑߩࠬ࠙ࡑޔߦ߆⏕ޕ‬࠻ࠍჇ᏷ߔࠆߚ߼ߩ PCR ࡊ࡜ࠗࡑ࡯࠮࠶࠻ߪ‫ ߽ߡ޿߅ߦ࠻࠶࡜ޔ‬PCR ↥‛߇ᓧࠄࠇ‫࡜ޔ‬࠶࠻♽⛔㑆ߦᄙဳ߽⷗ߟ߆ߞߚ㧔ᢥ₂ 11㧕‫࡯ࠞ࡯ࡑ࠻ࠗ࡜࠹ࠨࡠࠢࠗࡑߩࠬ࠙ࡑޔߒ߆ߒޕ‬ߩߔߴߡ߇‫ߦ࠻࠶࡜ޔ‬ㆡ↪ߢ߈ࠆࠊߌߢߪߥ߆ߞߚ‫ޕ‬ߘߎߢ‫⋥ޔ‬ធ⊛ߦ࡜࠶࠻ㆮવሶߩࠪ࡯ࠢࠛࡦࠬ࠺࡯࠲߆ࠄࡑࠗࠢࡠࠨ࠹࡜ࠗ࠻㗔ၞࠍ᛽಴ߒߡᣂⷙߩ࡜࠶࠻ㆮવሶࡑ࡯ࠞ࡯ࠍ㐿⊒ߒߚ޿ߣᕁߞߚ‫⇐ޕ‬ቇਛߦࡠࡦ࠼ࡦߢ㐿௅ߐࠇߚ࡜࠶࠻ળ⼏ߦෳടߒߚ㓙‫ ߩ࡯ࠡ࡞ࡌޔ‬Joziane Szpirer ඳ჻߆ࠄࡄ࡝ߩࡅ࠻ᄙဳ⎇ⓥᚲߩ
Mark Lathrop ඳ჻ߩ࠴࡯ࡓ߇ߘߩࠃ߁ߥ⹜ߺࠍߒߡ޿ࠆߣᢎ߃ߡߊࠇߚ‫ߦ࡝ࡄޕ‬ᚯࠆ߿
Lathrop ඳ჻ࠍ⸰໧ߒߡ‫ ߚ޿ߡ᧪ࠄ߆࡞ࠛ࡜ࠬࠗޔ‬Jacques Beckmann ඳ჻ߦ࡜࠶࠻ㆮવ
ሶࠪ࡯ࠢࠛࡦࠬ࠺࡯࠲ࡌ࡯ࠬߩ⺞ᩏ⁁ᴫࠍ⋥ធ⷗ߖߡ߽ࠄ޿‫౒ޔ‬ห⎇ⓥࠍᆎ߼ࠆߎߣߦߒ

ߚ‫ߩߘޕ‬ᓟߪ‫⎇࡞࡯࠷ࠬࡄޔ‬ⓥᚲߣࡅ࠻ᄙဳ⎇ⓥᚲߩਔᣇߦ಴౉ࠅߔࠆߎߣߦߥࠅ‫ޔ‬ᄙᔔߥᲤᣣ߇ᆎ߹ߞߚ‫⎇࡞࡯࠷ࠬࡄޕ‬ⓥᚲߢߪ‫ޔ‬Guénet వ↢‫ޔ‬ഥᚻߩ Xavier Montagutelli ඳ჻‫⎇ޔ‬ⓥഥᚻߩ Dominique Simon-Chazottes ߐࠎߦᄢᄌ߅਎⹤ߦߥߞߚ‫ޕ‬Ფᣣᔔߒ޿ߣߪ
⸒߃‫⎇ޔ‬ⓥߛߌࠍߒߡ߅ࠇ߫⦟޿ߩߛ߆ࠄ‫ޔ‬᳇ᭉߢᭉߒ޿߽ߩߢ޽ߞߚ‫ޕ‬ኅᣖߣ౒ߦᣣᧄ
ੱቇᩞߩ޽ࠆࡌ࡞ࠨ࡙ࠗㄭߊߩ৻イኅߦ૑ߺ‫ޔ‬ㅳᧃߪ‫ߣࠣࡦࡇ࠶࡚ࠪߢ࡯ࠞ࠲ࡦ࡟ޔ‬ዊᣏⴕࠍᭉߒࠎߛ‫ޕ‬੹ߢߪ᥉ㅢߦ೑↪ߐࠇߡ޿ࠆ 96 ⓣߩ PCR ⵝ⟎߇಴ᆎ߼ߚ㗃ߢ‫࡞ࡈࠍࠇߘޔ‬Ⓙ௛ߐߖߡ⸃ᨆࠍⴕߞߚ‫ޕ‬ᄞߩਛߦ߽㔚᳇ᵒേᓟߩࡃࡦ࠼߇ᤊⓨߩߏߣߊ಴ߡ߈ߚ‫ޕ‬੩ᄢ࠴࡯ࡓ߇૞
⵾ߒߚ࡜࠶࠻♽⛔㑆ߩ੤㈩࠺࡯࠲ߣࠥࡁࡓࠨࡦࡊ࡞‫߮ࠃ߅ޔ‬ᄢቇ㒮↢ߩ቟ᳯᱜ᣿ߐࠎ߇૞
⵾ߒߚࡑ࠙ࠬߣ࡜࠶࠻૕⚦⢩ࠢࡠ࡯ࡦࡄࡀ࡞ࠍᵴ↪ߒߡ‫ࠍ࠻ࠗ࡜࠹ࠨࡠࠢࠗࡑ࠻࠶࡜ޔ‬೑↪ߒߚ਎⇇ߢᦨೋߩᨴ⦡૕⇟ภ߇ઃ޿ߚ࡜࠶࠻ㆮવሶ࿾࿑ߩၮ⋚ࠍ૞⵾ߒߚ‫ޕ‬ᚒ‫ࡠ࡯࡛ޘ‬࠶ࡄߣߩ౒ห⎇ⓥࠣ࡞࡯ࡊߪ Nature ⹹㧔ᢥ₂ 12㧕ߦ‫☨ޔ‬࿖ࠣ࡞࡯ࡊߪ Cell ⹹ߦ㜞ⴊ࿶ࡕ࠺࡞࡜࠶࠻ߦ߅ߌࠆⴊ࿶ᗵฃᕈㆮવሶߩࡑ࠶ࡇࡦࠣ⺰ᢥࠍ‫߷߶ޔ‬หᤨߦ⊒⴫ߒߚ‫ޔߡߒߘޕ‬࡜࠶࠻ߩㆮવሶ࿾࿑߅ࠃ߮࡜࠶࠻࡮ࡑ࠙ࠬ࡮ࡅ࠻Ყセㆮવሶ࿾࿑ߣߒߡ➙߼ߚ⺰ᢥߪ‫ޔ‬ᣣᧄ࠴࡯ࡓ㧔ᐶᧄ㜞ᔒߐࠎ‫ޔ‬᫪ ᡽ਯߐࠎ‫ޔ‬ጊ↰ᷕਃవ↢ߣ⑳㧕ߣ࡛࡯ࡠ࠶ࡄ࠴࡯ࡓߦࠃࠅ
Genetics ⹹ߦ⊒⴫ߒߚ㧔ᢥ₂ 13㧕‫ࠇߘޕ‬એ㒠ߪ‫࠻࠶࡜ޔ‬ㆮવሶ࿾࿑ߣᲧセࠥࡁࡓ࿾࿑ࠍ㜞ኒᐲߥ߽ߩߣߔࠆߎߣߦജ߇ᵈ߇ࠇߚ㧔ᢥ₂ 14㧕‫ޔߦࠄߐޕ‬ᨴ⦡૕ߩ૏⟎ᖱႎࠍᜬߟㆮવሶ
࿾࿑ࠍ૞⵾㧔ᢥ₂ 15㧕ߒߚ㗃ߦߪ‫ߩ࡯ࠞ࡯ࡑ࠻ࠗ࡜࠹ࠨࡠࠢࠗࡑࠬ࠙ࡑߦ⊛⟜✂ޔ‬㐿⊒߇☨࿖ߢㅴߺ‫ޔ‬BN ࡜࠶࠻ߩోࠥࡁࡓࠪ࡯ࠢࠛࡦࠬࡊࡠࠫࠚࠢ࠻ߦⴕ߈⌕޿ߚ‫ޕ‬࡜࠶࠻ߩㆮવᄙဳࡑ࡯ࠞ࡯‫ޔ‬ㆮવሶ࿾࿑‫ޔ‬Ყセࠥࡁࡓ࿾࿑߇ᢛ஻ߐࠇࠆߣ‫⥄ޔ‬ὼ⊒∝ࡒࡘ࡯࠲ࡦ࠻࡜࠶࠻ߩේ࿃ㆮવሶߩᨴ⦡૕ࡑ࠶ࡇࡦࠣ߇ㅴߺ‫ࠣࡦ࠾࡯ࡠࠢ࡞࠽࡚ࠪࠫࡐޔ‬ᴺ޽ࠆ޿ߪࡐ࡚ࠫࠪ࠽࡞୥⵬ㆮવሶត⚝ᴺߦࠃࠅේ࿃ㆮવሶߩᄌ⇣ࠍ‫⑳ޔ‬㆐ߩ⎇ⓥ࠴࡯ࡓߢᰴ‫ߣޘ‬᣿ࠄ߆ߦߒߚ‫ޔߡߒߘޕ‬ኻ⽎޽ࠆ޿ߪ㑐ㅪߔࠆࡅ࠻∔ᖚߩ⊒∝ᯏ᭴ߩ⸃᣿ߦᄢ߈ߊ
⽸₂ߒߚ‫ޕ‬TRM ࡜࠶࠻
㧔Chr 10, Aspa ઁ, ᢥ₂ 16㧕
‫ޔ‬
ZI/mv ࡜࠶࠻(Chr 3, Atrn, ᢥ₂ 17; ᢥ₂ 18)‫ޔ‬
KDP ࡜࠶࠻㧔Chr 11, Cblb, ᢥ₂ 19㧕
‫ޔ‬KZC ࡜࠶࠻㧔Chr 4, Reln, ᢥ₂ 20㧕‫ޔ‬beige ࡜࠶࠻㧔Chr 17, Lyst, ᢥ₂ 21㧕
‫ޔ‬TM ࡜࠶࠻㧔Chr 1, Rab38, ᢥ₂ 22㧕‫ޔ‬cvd/hob ࡜࠶࠻㧔Chr 2,

Unc5h3, ᢥ₂ 23㧕‫ޔ‬qc ࡜࠶࠻㧔Chr 13, Lmx1a, ᢥ₂ 24㧕‫ޔ‬dfk ࡜࠶࠻㧔Chr 1, Kcnq1, ᢥ
₂ 25㧕‫ޔ‬SCR ࡜࠶࠻
㧔Chr 15, Fdft1; Chr20, Lss; ᢥ₂ 26㧕‫ޔ‬
groggy ࡜࠶࠻㧔Chr 19, Cacna1a,
ᢥ₂ 27㧕
‫ޔ‬dmy ࡜࠶࠻㧔Chr 17, Mrs2, ᢥ₂ 28㧕
‫ޔ‬swh ࡜࠶࠻㧔Chr 17, Edaradd, ᢥ₂29㧕‫ޕ‬৻ᣇ‫⎇ޔ‬ⓥቶߦᚯߞߡ߈ߚ⌀ਅ⍮჻ߐࠎ߇వዉᓎߦߥࠅ‫ޔ‬ᣂⷙߩ∔ᖚࡕ࠺࡞࡜࠶࠻ࠍ㐿⊒ߔࠆࡊࡠࠫࠚࠢ࠻ࠍផߒㅴ߼ߚ‫ޔߕ߹ޕ‬ൻቇᄌ⇣ේࠛ࠴࡞࠾࠻ࡠ࠰࠙࡟ࠕ⺃⊒ࡒࡘ࡯࠲ࠫࠚࡀࠪࠬߦࠃࠅ‫ ⚂ޔ‬1 ਁ࠮࠶࠻ߩ♖ሶߣ DNA ߩ࠮࠶࠻߆ࠄߥࠆ Kyoto University Rat

Mutant Archive (KURMA) ࠍ૞⵾ߒߡ‫ޔ‬DNA ߩᄌ⇣ࠬࠢ࡝࡯࠾ࡦࠣᴺ㧔MuT-POWER ᴺ‫ޔ‬
ᢥ₂ 30㧕ߣ㗼ᓸ᝼♖ᴺߦࠃࠅᣂⷙ∔ᖚࡕ࠺࡞࡜࠶࠻ࠍ⎇ⓥ⠪ߩⷐᦸߦࠃࠅ㐿⊒ߔࠆࠪࠬ࠹
ࡓࠍ᭴▽ߒߚ‫⑳ޔߡߒߘޕ‬㆐ߩ⎇ⓥ࠴࡯ࡓߩࡔࡦࡃ࡯߿౒ห⎇ⓥ⠪ߩ⥝๧ߦࠃࠅ૞⵾ߒߚ‫ޔ‬‫ޔ‬ᄢ⣺߇ࠎ㧔Apc ㆮવሶ, ᢥ₂ 31, 32, 33㧕‫ޔ‬ᾲᕈߌ޿ࠇࠎ㧔Scn1a ㆮવሶ, ᢥ₂ 34, 35, 36㧕ߡࠎ߆ࠎ㧔Lgi1 ㆮવሶ, ᢥ₂ 37㧕‫߮ࠃ߅ޔ‬㜞⢽ⴊ∝㧔Ldlr ㆮવሶ, ᢥ₂ 38㧕ߩ࡜࠶࠻ࡕ࠺
࡞ߦߟ޿ߡ‫⸃ޔ‬ᨆ࡮ᔕ↪⎇ⓥߩᚑᨐࠍ⊒⴫ߒߚ‫ޔߦࠄߐޕ‬⣕Ძ‫ࡁࠗ࡜ޔ‬᭽⊹⤏‫ޔ‬Ꮍ⁁♻⁁૕⎬ൻ∝ࠍ␜ߔ࡜࠶࠻߿⊒૞ᕈㆇേᄬ⺞∝ Episodic ataxia type 1 ߩࡕ࠺࡞ߣᕁࠊࠇࠆ࡜
࠶࠻߇ KURMA ߩ૞⵾ㆊ⒟ߦ߅޿ߡ⷗಴ߐࠇ‫ࠅࠃߦࠣࡦ࠾࡯ࡠࠢ࡞࠽࡚ࠪࠫࡐޔ‬೨⠪ߦ

Hr ㆮવሶᄌ⇣㧔ᢥ₂ 39㧕‫ޔ‬ᓟ⠪ߦ Kcna1 ㆮવሶᄌ⇣㧔ᢥ₂ 40㧕ࠍ‫ࠇߙࠇߘޔ‬หቯߒߚ‫ޕ‬ᰴ޿ߢ‫ੱޔ‬Ꮏ࠿ࠢ࡟࡯ࠕ࡯࠯ ZFN ࠍ↪޿ࠆᣇᴺߦࠃࠅ‫ޔ‬Ir2rg ߣ Prkdc ࠍᮡ⊛ߦߒߡ㊀ᐲ఺∉ਇో࡜࠶࠻ࠍ૞⵾ߒߚ㧔ᢥ₂ 41, 42㧕
‫ ߥ߁ࠃߩߎޕ‬ES ⚦⢩ࠍ੺ߐߥ޿ᣂⷙߩㆮવሶ
ᡷᄌᛛⴚߪ‫ޔ‬TALEN ᴺ‫ ߦࠄߐޔ‬CRISPR/Cas ࠪࠬ࠹ࡓ߳ߣ‫ޔ‬ᕆㅦߦ⊒ዷߒߡ޿ࠆ‫ࠇߎޕ‬ࠄߩᣂᛛⴚߦ߅޿ߡߪ‫ޔ‬ㆬᛮ⢒⒳ߦࠃߞߡ㐿⊒ߐࠇߚ∔ᖚࡕ࠺࡞࡜࠶࠻ࠍኻ⽎ߦߒߡ‫⋥ޔ‬ធ‫ޔ‬ᮡ⊛ㆮવሶࠍ৻ߟ޽ࠆ޿ߪⶄᢙหᤨߦᠲ૞ߔࠆߎߣ߇ߢ߈ࠆߩߢᄢᄌ㝯ജ⊛ߢ޽ࠆ‫ޕ‬㧟㧕࠽࡚ࠪ࠽࡞ࡃࠗࠝ࡝࠰࡯ࠬࡊࡠࠫࠚࠢ࠻‫ޠ࠻࠶࡜ޟ‬㧔0$424CV㧕ᐔᚑ  ᐕ  ᦬ߦ‫࡜ޔߜ߁ߩߘޔ߇ߚߒ⿷⊒߇࠻ࠢࠚࠫࡠࡊࠬ࡯࠰࡝ࠝࠗࡃ࡞࠽࡚ࠪ࠽ޔ‬࠶࠻ߩਛᩭᯏ㑐ߣߒߡ㒝ዻേ‛ታ㛎ᣉ⸳߇ㆬ߫ࠇ‫ᦼ  ╙ޔ‬㧔ᐔᚑ 㨪 ᐕᐲ㧕ߣ╙  ᦼ㧔ᐔᚑ 㨪 ᐕᐲ㧕ߪ‫⧔ޔ‬Ꮉࠍ⺖㗴▤ℂ⠪ߣߒߡ‫ߩ⛔♽࠻࠶࡜ޔ‬෼㓸‫଻ޔ‬ሽ‫ޔ‬ឭଏߩ੐ᬺࠍㆇ
༡ߒߚ㧔ᢥ₂ 㧕‫ޕ‬ᐔᚑ  ᐲ߆ࠄߩ╙  ᦼߪᐶᧄಎᢎ᝼߇⺖㗴▤ℂ⠪ࠍᜂ޿‫⃻ޔ‬࿷‫ޔ‬⌀ਅ․ቯಎᢎ᝼‫ޔ‬㊄ሶᱞੱ․ቯ⻠Ꮷ‫┻ޔ‬㢬⵨੫․ቯ⎇ⓥຬ‫⎇࠻ࠢ࡯ࠜࡈ࡯ࠟ࡞ࡆޔ‬ⓥຬ‫ޔ‬ศ⷗৻ੱ⎇ⓥຬ㧔ᐔᚑ  ᐕᐲ߆ࠄ㧕ࠄ߇㒝ዻേ‛ታ㛎ᣉ⸳ߩᛛⴚ♽⡯ຬߩᡰេࠍᓧߡၫⴕ
ߒߡ޿ࠆ‫ޔ߿੹ޕ‬෼㓸ߒߚ࡜࠶࠻ߩ♽⛔ᢙߪ  ♽⛔ࠍ⿥߃ࠆ਎⇇ᦨᄢⷙᮨߩ࡜࠶࠻࡝࠰
࡯ࠬ࠮ࡦ࠲࡯ߣߥࠅ‫ޔ‬࿖ౝᄖߩ࡜࠶࠻⎇ⓥߦ⽸₂ߒߡ޿ࠆ‫ޔࠎࠈߜ߽ޕ‬㘺⢒ࠬࡍ࡯ࠬߦ૛
⵨߇ߥ޿ߩߢ‫ޔ‬ㆬ೎ߒߚ♽⛔ߩߺ߇↢૕ߢ⛽ᜬߐࠇߡ߅ࠅ‫ߪ⛔♽ߩઁޔ‬⢦ߣ♖ሶߦࠃࠆ଻
ሽߣߒߡ‫ޔ‬ଐ㗬ߦᔕߓߡ୘૕ᓳᏫߒߡឭଏߒߡ޿ࠆ‫ޕ‬෼㓸ߒߚ࡜࠶࠻♽⛔ߩ૕㊀‫ⴕޔ‬േ‫ޔ‬ⴊ࿶‫ⴊޔ‬ᶧ‫ޔ‬ዩߥߤߦߟ޿ߡߩ  㗄⋡ߩᬌᩏ୯㧔ᢥ₂ 㧕ߣ &0# ᄙဳࡑ࡯ࠞ࡯55.2 ߦࠃࠆࠥࡁࡓࡊࡠࡈࠔࠗ࡞㧔ᢥ₂ 㧕ࠍ⺞ߴߡ‫⛔♽⥸৻ޔ‬ᖱႎߣ౒ߦ 0$424CV ߩࡎ࡯ࡓࡍ࡯ࠫ
ߦߡ౏㐿ߒߡ޿ࠆ㧔JVVRYYYCPKOOGFM[QVQWCELR0$4㧕‫ޕ‬ᣂⷙߩㆮવሶᡷᄌ࡜࠶࠻
߽㗅ᰴነ⸤ߒߡ‫ޔ‬ឭଏߢ߈ࠆࠃ߁ߦߒߡ޿ࠆ‫ߩ࠻࠶࡜ޕ‬ឭଏߣ଻ሽߦ㑐ㅪߔࠆᛛⴚ߿࡜࠶
࠻ࠥࡁࡓᖱႎߩᢛ஻߽ 0$42 ߩ౏൐⺖㗴ߦ೎ㅜណᛯߐࠇߡⴕߞߡ߈ߚ‫ޕ‬ታ㛎↪࡜࠶࠻ߩࠥࡁ
ࡓᖱႎၮ⋚ᢛ஻ߣߒߡ‫(ޔ‬5VO ߣ .'5VO ߩ $#% ࠢࡠ࡯ࡦ࡜ࠗࡉ࡜࡝࡯ߩ૞⵾‫┵ᧃߩߘޔ‬㈩೉ߩࠪ࡯ࠢࠛࡦࠬ‫ޔ‬ਗ߮ߦ‫ࠍࠬࡦࠛࠢ࡯ࠪࡓࡁࠥోޔ‬㗅ᰴⴕߞߚ‫(ޔߦ․ޕ‬5VO ߩࠥࡁࡓࠪ࡯ࠢࠛࡦࠬ࠺࡯࠲ߪ‫ޔ‬㕖Ᏹߦା㗬ᕈ߇㜞޿ߣ⹏ଔߐࠇ‫੹ޔ‬ᓟ‫ޔ‬ታ㛎↪࡜࠶࠻♽⛔ߩᮡḰࠥࡁࡓࠪ࡯ࠢࠛࡦࠬߦߥࠆߣᦼᓙߒߡ޿ࠆ‫ޕ‬㊄ሶᱞੱߐࠎߦࠃࠆࡈ࡝࡯࠭࠼࡜ࠗ♖ሶ
ߩ଻ሽᴺߩታ↪ൻ߽㊀ⷐߥᛛⴚၮ⋚⎇ⓥߩᚑᨐߢ޽ࠆ㧔ᢥ₂ 㧕‫ޕ‬㧠㧕⎇ⓥᵴേࠍᝄࠅ㄰ߞߡ
ጊ↰ᷕਃవ↢ߣߚߞߚੑੱߢᆎ߹ߞߚ⎇ⓥቶ߇੩ㇺᄢቇකቇ⎇ⓥ⑼࡮කቇㇱߩၮᐙ⻠ᐳ‫ޟ‬ታ㛎േ‛ቇ‫ޔࠅߥߣޠ‬ఝ⑲ߥඳ჻߿ୃ჻ࠍヘ಴ߔࠆߦ⥋ߞߚߎߣߪᄢᄌ༑߫ߒ޿‫ߎߎޕ‬ߦㅀߴߚ⎇ⓥᬺ❣ߪ‫ޔ‬ᢥ₂ߦ␜ߒߚߏߣߊታ㛎േ‛ቇᢎቶߩᱧઍߩࠬ࠲࠶ࡈ‫⎇ޔ‬ⓥ↢‫ޔ‬ᄢቇ㒮↢‫߮ࠃ߅ޔ‬࿖ౝᄖߩᄢᄌᄙߊߩᣇ‫౒ߩߣޘ‬ห⎇ⓥߦࠃࠆ߽ߩߢ޽ࠆ‫⊝ޕ‬᭽ߩᩰ೎ߩߏ
ദജߦᢘᗧࠍ⴫ߒ‫ߏޔߚ߹ޔ‬දജߦᔃࠃࠅᗵ⻢ࠍ↳ߒ਄ߍࠆ‫⎇ޔߡߒߘޕ‬ⓥᵴേࠍߏᡰេ
ߒߡ㗂޿ߚ㑐ଥᯏ㑐‫ޔ‬㑐ଥฦ૏ߦᷓߊᓮ␞ࠍ↳ߒ਄ߍࠆ‫ޕ‬߅ࠊࠅߦᧄⓂߪ‫ޔ‬ᐔᚑ  ᐕ  ᦬ߩ╙  ࿁㑐⷏ታ㛎േ‛⎇ⓥળߦ߅ߌࠆ⴫㗴ߩ⻠Ṷ⸥㍳ߣߒߡ➙߼ߚ߽ߩߢ޽ࠆ‫ޕ‬೨Ბߩ‫ޟ‬੩ㇺᄢቇߦ߅ߌࠆേ‛ታ㛎ߩ▤ℂᬺോ‫ޔߪߡ޿ߟߦޠ‬੩ㇺᄢቇකቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳ႎ╙ 10 ภߦၫ╩ߒߚ‫ޟ‬㒝ዻേ‛ታ㛎ᣉ⸳ߩㆇ༡ 40 ᐕ‫߆ޠ‬ࠄ‫ޔ‬ᓟᲑߩ‫ޟ‬੩ㇺᄢቇߢߩ⎇ⓥᵴേ‫ޔߪߡ޿ߟߦޠ‬ቯᐕㅌ⡯ᤨߦ➙߼ߚ⎇ⓥᬺ❣㓸ߩ‫⎇ޟ‬ⓥᵴേࠍᝄࠅ㄰ߞߡ‫ߩࠄ߆ޠ‬ౝኈࠍขࠅㄟߺ‫ޔ‬ട╩‫ޔ‬ᡷ⸓ߒߚ‫ޕ‬
ᢥ₂㧝㧚⧔Ꮉᔘᄦ‫ޔ‬ጊ↰ᷕਃ㧦࠮ࡒࡃ࡝ࠕᣇᑼߩࡑ࠙ࠬ‫౒࠻࠶࡜ޔ‬ห೑↪ታ㛎ᣉ⸳ߦ߅ߌࠆ࠮
ࡦ࠳ࠗ࠙ࠗ࡞ࠬᳪᨴ੐ߣߘߩኻ╷‫ޔ‬ਛᎹ㓷㇢ઁ✬‫ޔ‬ታ㛎േ‛ᣉ⸳ߩᓸ↢‛ࠦࡦ࠻ࡠï࡞ߣߘߩታ㓙‫ޔ‬p161-171‫ޔ‬ᷡ⥋ᦠ㒮‫᧲ޔ‬੩‫ޔ‬1984
㧞㧚Serikawa T, Kitada K, Muraguchi T, Yamada J: A survey of Pneumocystis carinii
infection in research mouse colonies in Japan. Lab Anim Sci, 41: 411-414, 1991
㧟㧚⧔Ꮉᔘᄦ‫ޔ‬ጊ↰ᷕਃ㧦‫⾗ޟ‬ᢱ‫ޠ‬੩ㇺᄢቇකቇㇱߦ߅ߌࠆ࡜࠶࠻߅ࠃ߮ࡅ࠻ߩ⣢∝୥ᕈ಴ⴊᾲ࠙ࠗ࡞ࠬ᛫૕㓁ᕈ଀ߩᬌ಴ߣߘߩኻ╷ߦ㑐ߔࠆႎ๔‫ޔ‬Exp Anim 42: 665-671,
1993
㧠㧚Serikawa T, Muraguchi T, Nakao N: A survey of dogs from Gifu and Shiga areasfor Brucella canis. Jpn J Vet Sci 39: 635-642, 1977
㧡㧚Serikawa T, Muraguchi T, Yamada J, Takada H: Sperm agglutination and sperm
agglutinating activity in male dogs experimentally infected with Brucella canis.

Jpn J Vet Sci 43: 469-490, 1981
㧢㧚Serikawa

T,

Kondo

Y,

TakadaH,

YamadaJ:

Head-to-head

type

auto-spermagglutination with IgA antibody to acrosome induced by Brucella canis
infection. Jpn J Vet Sci 46: 41-48, 1984
㧣㧚Yamada J, Serikawa T, Ishiko J, Inui T, Takada H, Kawai Y, Okaniwa A: Rats
with congenital tremor and curled whiskers and hair. Exp Anim 34: 183-188, 1985
㧤㧚Serikawa T, Yamada J: Epileptic seizures in rats homozygous for two mutations,zitter and tremor. J Hered 77: 441-444, 1986

㧥㧚Sasa M, Ohno Y, Ujihara H, Fujita Y, Yoshimura M, Takaori S, Serikawa T,
Yamada J: Effects of antiepileptic drugs on absence-like and tonic seizures in the
spontaneously epileptic rat, a double mutant rat. Epilepsia 29: 505-513, 1988
㧝㧜㧚Mori M, Ishizaki K, Yamada T, Chen H, Sugiyama T, Serikawa T, Yamada J:
Restriction fragment length polymorphisms of the angiotensinogen gene in inbred
rat strains and mapping of the gene on chromosome 19q. Cytogenet Cell Genet 50:
42-45, 1989
㧝㧝㧚Kondo Y, Mori M, Kuramoto T, Yamada J, Beckmann JS, Simon-Chazottes D,
Montagutelli X, Guénet JL, Serikawa T: DNA segments mapped by reciprocal useof microsatellite primers between mouse and rat. Mamm Genome 4: 571-576, 1993㧝㧞㧚Hilbert P, Lindpaintner K, Beckmann JS, Serikawa T, Soubrier F, Dubay C,
Cartwright P, De Gouyon B, Julier C, Takahashi S, Vincent M, Georges D,
Lathrop MG: Chromosomal mapping of two genetic loci associated withblood-pressure regulation in hereditary hypertensive rats. Nature 353: 521-529,
1991
㧝㧟㧚Serikawa T, Kuramoto T, Hilbert P, Mori M, Yamada J, Dubay CJ,
Lindpaintner K, Ganten D, Guénet JL, Lathrop GM, Beckmann JS: Rat gene
mapping using PCR-analyzed microsatellites. Genetics, 131: 701-721, 1992
㧝㧠㧚Yamada J, Kuramoto K, Serikawa T: A rat genetic linkage map andcomparative maps for mouse or human homologus rat genes. Mamm Genome 5:
63-83, 1994
㧝㧡㧚Andoh Y, Kuramoto T, Yokoi N, Maihara T, Kitada K, Serikawa T: Correlation
between genetic and cytogenetic maps of the rat. Mamm Genome, 9: 287-293,
1998
㧝㧢㧚Kitada K, Akimitsu T, Shigematsu Y, Kondo A, Maihara T, Yokoi N, Kuramoto
T, Sasa M, Serikawa T: Accumulation of N-acetyl-L-aspartate in the brain of thetremor rat, a mutant exhibiting absence-like seizure and spongiform degenerationin the central nervous system. J Neurochem 74: 2512-2519, 2000
㧝㧣㧚Kuramoto T, Kitada K, Inui T, Sasaki Y, Ito K, Hase T, Kawaguchi S, Ogawa Y,
Nakao K, Barsh GS, Nagao M, Ushijima T, Serikawa T:Attractin/Mahogany/Zitter
plays a critical role in myelination of the central nervous system. Proc Natl Acad

Sci USA 98: 559-564, 2001
㧝㧤㧚Kuwamura M, Maeda M, Kuramoto T, Kitada K, Kanehara T, Moriyama M,
Nakane Y, Yamate J, Ushijima T, Kotani T, Serikawa T: The myelin vacuolation(mv) rat with a null mutation in the attractin gene. Lab Invest 82:1279-1286, 2002
㧝㧥㧚Yokoi N, Komeda K, Wang HY, Yano H, Kitada K, Saitoh Y, Seino Y, Yasuda K,

Serikawa T, Seino S: Cblb is a major susceptibility gene for rat type 1 diabetes
mellitus. Nature Genet 31: 391-394, 2002
㧞㧜㧚Yokoi N, Namae M, Wang HY, Kojima K, Fuse M, Yasuda K, Serikawa T, Seino
S, Komeda K: Rat neurological disease creeping is caused by a mutation in thereelin gene. Brain Res Mol Brain Res 112:1-7, 2003
㧞㧝㧚Mori M, Yamasaki K, Nakanishi S, Kitada,K, Higuchi K, Namiki C, Hamada S,
Serikawa T: A new beige mutant rat ACI/N-Lystbg-Kyo. Exp Anim, 52:31-36, 2003
㧞㧞㧚Oiso N, Riddle SR, Serikawa T, Kuramoto T, Spritz RA: The Rat Ruby (R)Locus is Rab38: Identical Mutations in Fawn-Hooded and Tester-Moriyama Rats
Derived from an Ancestral Long Evans Rat Sub-Strain. Mamm Genome 15: 307314, 2004
㧞㧟㧚Kuramoto T, Kuwamura M, Serikawa T: Rat neurological mutations cerebellar
vermis defect and hobble are caused by mutations in the netrin-1 receptor gene
Unc5h3. Brain Res Mol Brain Res 122: 103-108, 2004
㧞㧠㧚Kuwamura M, Muraguchi T, Matsui T, Ueno M, Takenaka S, Yamate J, Kotani
T, Kuramoto T, Guénet JL, Kitada K, Serikawa T: Mutation at the Lmx1a locus
provokes aberrant brain development in the rat. Brain Res Dev Brain Res 155:
99-106, 2005
㧞㧡㧚Gohma H, Kuramoto T, Kuwamura M, Okajima R, Tanimoto N, Yamasaki K,
Nakanishi S, Kitada K, Makiyama T, Akao M, Kita T, Sasa M, Serikawa T: WTC
deafness Kyoto (dfk): a rat model for extensive investigations of Kcnq1 functions.

Physiol Genomics 24: 198-206, 2006㧞㧢㧚Mori M, Li G, Abe I, Nakayama J, Guo Z, Sawashita J, Ugawa T, Nishizono S,
Serikawa T, Higuchi K, Shumiya S: Lanosterol synthase mutations causecholesterol deficiency-associated cataracts in the Shumiya cataract rat. J Clin

Invest 116: 395-404, 2006㧞㧣㧚Tokuda S, Kuramoto T, Tanaka T, Kaneko S, Takeuchi IK, Sasa M, Serikawa T:
The ataxic groggy rat has a missense mutation in the P/Q-type voltage gated Ca2+
channel D1A subunit gene and exhibits absence seizures. Brain Res 1133: 168-177,
2007
㧞㧤㧚Kuramoto T, Kuwamura M, Tokuda S, Izawa T, Nakane Y, Kitada K, Akao M,
Guénet JL, Serikawa T: A mutation in the gene encoding mitochondrial Mg2+
channel MRS2 results in demyelination in the rat. PLoS Genet 7:e1001262, 2011㧞㧥㧚Kuramoto T, Yokoe M, Hashimoto R, Hiai H, Serikawa T: A rat model ofhypohidrotic ectodermal dysplasia carries a missense mutation in the Edaradd
gene. BMC Genetics 12: 91, 2011㧟㧜㧚Mashimo T, Yanagihara K, Tokuda S, Voigt B, Takizawa A, Nakajima R, Kato
M, Hirabayashi M, Kuramoto T, Serikawa T: An ENU-induced mutant archive forgene targeting in rats. Nat Genet 40: 514-515, 2008
㧟㧝㧚Yoshimi K, Tanaka T, Takizawa A, Kato M, Hirabayashi M, Mashimo T,
Serikawa T, Kuramoto T: Enhanced colitis-associated colon carcinogenesis in anovel Apc mutant rat. Cancer Sci 100: 2022-2027, 2009㧟㧞㧚Yoshimi K, Hashimoto T, Niwa Y, Hata K, Serikawa T, Tanaka T, Kuramoto T:
Use of a chemically induced-colon carcinogenesis-prone Apc-mutant rat in achemotherapeutic bioassay. BMC Cancer 12: 448, 2012㧟㧟㧚Yoshimi K, Tanaka T, Serikawa T, Kuramoto T: Tumor suppressor APC protein
is essential in mucosal repair from colonic inflammation through Angiogenesis.

Am J Pathol 182:1263-1274, 2013㧟㧠㧚Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M,
Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y,
Serikawa T: A missense mutation of the gene encoding voltage-dependent sodium
channel (Nav1.1) confers susceptibility to febrile seizures in rats. J Neurosci 30:
5744-5753, 2010㧟㧡㧚Ohno Y, Sofue N, Ishihara S, Mashimo T, Sasa M, Serikawa T: Scn1a missense
mutation impairs GABAA receptor- mediated synaptic transmission in the rat
hippocampus. Biochem Biophys Res Commun 400: 117-122, 2010
㧟㧢㧚Hayashi K, Ueshima S, Ouchida M, Mashimo T, Nishiki T, Sendo T, Serikawa T,
Matsui H, Ohmori I: Therapy for hyperthermia-induced seizures in Scn1a mutant
rats. Epilepsia 52:1010-1017, 2011㧟㧣㧚Baulac S, Ishida S, Mashimo T, Boillot M, Fumoto N, Kuwamura M, Ohno Y,
Takizawa A, Aoto T, Ueda M, Ikeda A, LeGuern E, Takahashi R, Serikawa T. A rat
model for LGI1-related epilepsies. Hum Mol Genet 21: 3546-3557, 2012㧟㧤㧚Asahina M, Mashimo T, Takeyama M, Tozawa R, Hashimoto T, Takizawa A,
Ueda M, Aoto T, Kuramoto T, Serikawa T. Hypercholesterolemia andatherosclerosis in low density lipoprotein receptor mutant rats. Biochem Biophys

Res Commun 418: 553-558, 2012㧟㧥㧚Kuramoto T, Kuwamura M, Tagami F, Mashimo T, Nose M, Serikawa T: Kyoto
rhino rats derived by ENU mutagenesis undergo congenital hair loss and exhibit
focal glomerulosclerosis. Exp Anim 60: 57-63, 2011.
㧠㧜㧚Ishida S, Sakamoto Y, Nishio T, Baulac S, Kuwamura M, Ohno Y, Takizawa A,
Kaneko S, Serikawa T, Mashimo T.: Kcna1-mutant rats dominantly display
myokymia, neuromyotonia and spontaneous epileptic seizures. Brain Res 1435:

154-166, 2012㧠㧝㧚Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T:
Generation of knockout rats with X-linked severe combined immunodeficiency(X-SCID) using zinc-finger nucleases. PLoS One 5: e8870, 2010㧠㧞㧚Mashimo T, Takizawa A, Kobayashi J, Kunihiro Y, Yoshimi K, Ishida S, Tanabe
K, Yanagi A, Tachibana A, Hirose J, Yomoda J, Morimoto S, Kuramoto T, Voigt B,
Watanabe T, Hiai H, Tateno C, Komatsu K, Serikawa T: Generation andcharacterization of severe combined immunodeficiency rats. Cell Rep 2: 685-694,
2012㧠㧟㧚Serikawa T: Colourful history of Japan's rat resources. Nature 429: 15, 2004㧠㧠㧚Serikawa T, Mashimo T, Takizawa A, Okajima R, Maedomari N, Kumafuji K,
Tagami F, Neoda Y, Otsuki M, Nakanishi S, Yamasaki K, Voigt B, Kuramoto T:
National BioResource Project-Rat and related activities. Exp Anim 58: 333-341.
2009
㧠㧡㧚Mashimo T, Voigt B, Kuramoto T, Serikawa T: Rat Phenome Project: theuntapped potential of existing rat strains. J Appl Physiol 98: 371-379, 2005
㧠㧢㧚Mashimo T, Voigt B, Tsurumi T, Naoi K, Nakanishi S, Yamasaki K, Kuramoto T,
Serikawa T: A set of highly informative rat simple sequence length polymorphism(SSLP) markers and genetically defined rat strains. BMC Genetics 7: 19, 2006
㧠㧣㧚Kaneko T, Serikawa T: Successful long-term preservation of rat sperm by
freeze-drying. PLoS One 7: e35043, 2012

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LGI1 ኚ␗ࣛࢵࢺ㡢่⃭ㄏⓎࡅ࠸ࢀࢇࣔࢹࣝ࡟࠾ࡅࡿ c-Fos ච␿ᰁⰍ࡟ࡼࡿゎᯒ‫ۑ‬㯄┤ᾈ 1,2ࠊ┿ୗ▱ኈ 1ࠊቑ஭῟ 3ࠊỈཱྀ♸Ⓩ 3ࠊ༡ᮏ⩧Ꮚ 3ࠊ▼⏣⣪ᜨᏊ 1ࠊụ⏣᫛ኵ 2ࠊ㧗ᶫ
Ⰻ㍜ 2ࠊ኱㔝⾜ᘯ 3ࠊⱂᕝᛅኵ 1 㸦1 ி኱㝔㺃་㺃ື≀ᐇ㦂᪋タࠊ2 ྠ࣭⮫ᗋ⚄⤒Ꮫࠊ3 ኱㜰⸆⛉኱Ꮫ࣭⸆ရస⏝ゎᯒᏛ㸧࠙⫼ᬒ࣭┠ⓗࠚࣄࢺᖖᰁⰍయඃᛶእഃഃ㢌ⴥ࡚ࢇ࠿ࢇ㸦ADLTE㸧ࡣ⫈ぬ⑕≧➼࠿ࡽጞࡲࡿ㒊
ศⓎసࢆ࿊ࡍࡿ㑇ఏᛶ㒊ศ࡚ࢇ࠿ࢇ࡛ࠊእഃഃ㢌ⴥ࡟↔Ⅼࡀᣦ᦬ࡉࢀࡿࠋLGI1 ࡣ ADLTE ࡢཎᅉ㑇ఏᏊࡢ୍ࡘ࡛ࠊࡇࢀࡲ࡛ᵝࠎ࡞ᤄධ࣭Ḟኻ࣭⨨᥮ኚ␗ࡀሗ࿌ࡉࢀ࡚࠸ࡿࠋ㏆ᖺࠊ┿ୗࡽࡣ Gene-driven ENU (N-ethyl-N-nitrosourea)࣑࣮ࣗࢱࢪ࢙ࢿࢩࢫἲ࡟ࡼࡾࠊLGI1 㑇ఏᏊ࡟
L385R ࣑ࢫࢭࣥࢫኚ␗ࢆࡶࡘࣛࢵࢺࢆస〇ࡍࡿࡇ࡜࡟ᡂຌࡋࡓࠋࡉࡽ࡟ࠊLGI1 ኚ␗ࣛࢵࢺࡣ
㡢่⃭࡛࡚ࢇ࠿ࢇⓎసࡀㄏⓎࡉࢀࡿࡇ࡜ࢆⓎぢࡋࡓࠋᮏ◊✲࡛ࡣࠊLGI1 ኚ␗ࣛࢵࢺࡢ㡢่⃭Ⓨస᫬࡟኱⬻࡛⯆ዧࡍࡿ㒊఩ࢆ᫂ࡽ࠿࡟ࡍࡿࡓࡵࠊ㟁ᴟᇙ㎸ࡳ⬻Ἴࡸ⚄⤒⯆ዧࡢ࣐࣮࣮࢝⺮ⓑ࡛࠶ࡿ c-Fos ࡢච␿ᰁⰍゎᯒࢆ᪋⾜ࡋࡓࠋࡲࡓࠊⓎసࡢ࣓࢝ࢽࢬ࣒ࢆゎ᫂ࡍࡿࡓࡵ mRNA ࣐࢖ࢡࣟ࢔ࣞ࢖㑇ఏᏊⓎ⌧ゎᯒࢆ⾜࡞ࡗࡓࠋ࠙᪉ἲࠚLGI1 ኚ␗㸦࣊ࢸࣟ㸧ࣛࢵࢺ࡜ᑐ↷⩌ࡢ F344 ࣛࢵࢺ࡟ࡘ࠸࡚ࠊ
㡢่⃭↓ࡋࡢ A ⩌㸦LGI1
ኚ␗ n=8ࠊF344 n=6㸧
ࠊ16 ᪥㱋ࣉࣛ࢖่࣒⃭(120 dB, 10kHz, 1min.)ࡢࡳࡢ B ⩌㸦ྛ n=7,15㸧ࠊࣉࣛ࢖่࣒⃭ཬࡧ 8 㐌㱋㡢่⃭(120dB, 10kHz, 5 min.) ࡢ C ⩌㸦ྛ n=7, 7㸧࡟ศࡅࡓࠋ
AࠊB ⩌ࡣ 8 㐌㱋᫬࡟⬻ࢆ᥇ྲྀࡋࠊC ⩌ࡣ 8 㐌㱋᫬ࡢ㡢่⃭ 2 ᫬㛫ᚋ࡟⬻᥇ྲྀࡋࡓࠋ㸲㸣ࣃ࣒ࣛ࣍ࣝ࢔ࣝࢹࣄࢻ࡛ᅛᐃᚋࠊ⬻⤌⧊ษ∦࡟࠾࠸࡚ c-Fos ච␿ᰁⰍࢆ⾜࠸ࠊ඲⬻ 34 㒊఩࡛ᰁⰍ㝧ᛶ⣽⬊ᩘࢆィ

ࡋࡓࠋLGI1 ࡀ࡚ࢇ࠿ࢇཎᛶ࡟㛵୚ࡍࡿ࣓࢝ࢽࢬ࣒ࢆ᭦࡟᳨ウࡍࡿࡓࡵࠊୖ

グ㒊఩࡟࡚ mRNA Ⓨ⌧㔞ࡢ࣐࢖ࢡࣟ࢔ࣞ࢖ゎᯒࢆ᪋⾜ࡋࡓࠋ࠙⤖ᯝ࣭⪃ᐹࠚࣉࣛ࢖่࣒⃭ཬࡧ 8 㐌㱋㡢่⃭ࡢ C ⩌࡟࠾࠸࡚ࠊLGI1 ኚ␗ࣛࢵࢺ඲㢌ࡀ wild
running ᚋ࡟ᙉ┤㛫௦Ⓨస(GTCS)ࢆ♧ࡋࡓࡀࠊF344 ࣛࢵࢺࡣ wild running ࡢࡳࢆ♧ࡋࡓࠋⓎస᫬⬻Ἴ࡛ࡣࠊእഃഃ㢌ⴥ࡜๓㢌ⴥ࡜࡛࡚ࢇ࠿ࢇᛶᨺ㟁Ⓨ⏕࡟᫬㛫ᕪࢆㄆࡵ࡞࠿ࡗࡓࠋc-Fosච␿ᰁⰍゎᯒࡢ⤖ᯝࠊLGI1 ኚ␗ࣛࢵࢺ࡛ࡣ F344 ࣛࢵࢺ࡜ẚ㍑ࡋ࡚ 8 㐌㱋᫬ࡢ㡢่⃭࡟ࡼࡾࠊ⫈ぬ⓶㉁ࠊႥ࿘⓶㉁ࠊႥෆ⓶㉁ࠊឤぬ⓶㉁ࠊ↓㢛⢏ᛶᓥ⓶㉁࡛ᰁⰍ⣽⬊ᩘ࡟᭷ព࡞ୖ᪼ࢆㄆࡵ
ࡓࠋࡇࢀࡽࡢ⤖ᯝࡣࠊࣄࢺ ADLTE ࡛እഃഃ㢌ⴥࡢ␗ᖖⓎⅆࡀᣦ᦬ࡉࢀ࡚࠸ࡿ஦ᐇࢆ⪃៖ࡍࡿ
࡜ࠊADLTE ື≀ࣔࢹࣝ࡜ࡋ࡚ࡢ LGI1 ኚ␗ࣛࢵࢺࡢ᭷⏝ᛶࢆ♧၀ࡋ࡚࠸ࡿࠋࡲࡓࠊLGI1 ኚ␗ࣛࢵࢺ࡛㡢่⃭ㄏⓎ GTCS ᚋ࡟ c-Fos ᰁⰍ⣽⬊ᩘࡀቑຍࢆ♧ࡋࡓ㒊఩ࡣࠊᚑ᮶ሗ࿌ࡉࢀ࡚࠸ࡿ⫈ぬᛶࢽ࣮ࣗࣟࣥࢿࢵࢺ࣮࣡ࢡ࡜▩┪ࡋ࡚࠾ࡽࡎࠊ㸰ḟᛶࡢ GTCS Ⓨ⑕࣓࢝ࢽࢬ࣒࡜ࡢ
㛵㐃ᛶࡀ♧ࡉࢀࡿࠋ࣐࢖ࢡࣟ࢔ࣞ࢖ゎᯒ࡛ࡣࠊLGI1 㛵㐃㑇ఏᏊࡣ LGI1 ኚ␗ࡸⓎసࡢ᭷↓࡛ࡢኚືࢆㄆࡵ࡞࠸୍᪉࡛ࠊ࡚ࢇ࠿ࢇ࡜ࡢ㛵㐃ࡀሗ࿌ࡉࢀࡓ 8 ࡘࡢ㑇ఏᏊࡀ LGI1 ኚ␗ࣛࢵࢺࡢ
Ⓨస࡟క࠸ኚືࡋࡓࠋ⌧ᅾࠊࡇࢀࡽ࡚ࢇ࠿ࢇ㛵㐃㑇ఏᏊ࡜ LGI1 ኚ␗ࣛࢵࢺ࡟࠾ࡅࡿ㡢่⃭ㄏ
Ⓨࡅ࠸ࢀࢇⓎ⑕࣓࢝ࢽࢬ࣒࡟ࡘ࠸᳨࡚ウࡋ࡚࠸ࡿࠋ

2

TRM ࣛࢵࢺ࡟࠾ࡅࡿḞ⚄࡚ࢇ࠿ࢇࡢ㑇ఏせᅉ‫ۑ‬ᗜᮏ㧗ᚿࠊᶓỤ⧛Ꮚࠊⱂᕝᛅኵ㸦ி㒔኱㝔࣭་࣭ື≀ᐇ㦂᪋タ㸧
TRM ࣛࢵࢺࡣࠊ୙ዷࠊ⿕ẟ␗ᖖࠊᮏែᛶ᣺ᡓࠊḞ⚄Ⓨసࢆ♧ࡍ࣑࣮ࣗࢱ
ࣥࢺ⣔⤫࡛࠶ࡿࠋள⣔⤫ࡢ TRMR ࣛࢵࢺࡣࠊ୙ዷ࡜⿕ẟ␗ᖖࡣ♧ࡍࡀࠊᮏែᛶ᣺ᡓࡸḞ⚄Ⓨసࢆ♧ࡉ࡞࠸ࠋᡃࠎࡣࡇࢀࡲ࡛࡟ࠊTRM ࡜ TRMR ࢆ⏝࠸ࡓ㑇ఏゎᯒ࠿ࡽࠊᮏែᛶ᣺ᡓࡢ㑇ఏせᅉ࡜ࡋ࡚ࠊK+ࢳࣕࢿ࡛ࣝ࠶ࡿ
Hcn1 㑇ఏᏊࡢ࣑ࢫࢭࣥࢫኚ␗㸦ࢳࣕࢿࣝᶵ⬟႙ኻ㸧࡜࢔ࢭࢳࣝ࢔ࢫࣃࣛ
ࢠࣥ㓟
㸦NAA㸧
ࡢศゎ㓝⣲࡛࠶ࡿ Aspa 㑇ఏᏊࡢḞኻኚ␗ࢆぢฟࡋࡓࠋ
TRM⣔ࡣࡇࡢ୧ኚ␗ࢆ᭷ࡍࡿࡓࡵ࡟ࠊᮏែᛶ᣺ᡓࢆⓎ⑕ࡍࡿࡀࠊTRMR ⣔ࡣ
Aspa ኚ␗ࡢࡳ᭷ࡍࡿࡢ࡛ᮏែᛶ᣺ᡓࢆⓎ⑕ࡋ࡞࠸ࠋ
௒ᅇࠊḞ⚄Ⓨస࡟ࡘ࠸࡚ࡶࠊྠᵝࡢゎᯒࢆ⾜ࡗࡓࠋ(TRM x TRMR)F1 x
TRM ᡠࡋ஺㞧Ꮚ 25 㢌࡟ࡘ࠸࡚ࠊ15 㐌㱋᫬࡟◳⭷ୖ࡟ᇙࡵ㎸ࢇࡔ㟁ᴟ࠿ࡽ⬻Ἴࢆ ᐃ
㸦30 ศ㛫ࢆ 2 ᅇ㸧ࡋࡓࠋ⬻Ἴୖࡢ spikes-and-waves discharge(SWD) ࢆḞ⚄ⓎసⓎ⑕ࡢᣦᶆ࡜ࡋࠊฟ⌧ᅇᩘ࡜ᣢ⥆᫬㛫ࢆ ᐃࡋࡓࠋ
Ḟ⚄Ⓨసࢆ♧ࡍಶయࡣ 14 㢌ࠊ♧ࡉ࡞࠸ಶయࡣ 11 㢌࡛࠶ࡾࠊḞ⚄Ⓨసࡢ᭷↓࡜ Hcn1 ኚ␗ࡢ᭷↓ࡣ᏶඲࡟୍⮴ࡋ࡚࠸ࡓࠋࡲࡓࠊAspa/Hcn1 ࢲࣈࣝ
KO ࣐࢘ࢫࢆస〇ࡋࠊࡑࡢ⬻Ἴࢆ ᐃࡋࡓ࡜ࡇࢁࠊSWD ࡀほᐹࡉࢀࡓࠋ
௨ୖࡢࡇ࡜࠿ࡽࠊAspa ኚ␗ࡢࡳࠊHcn1 ኚ␗ࡢࡳ࡛ࡣḞ⚄ⓎసࡣⓎ⑕ࡏࡎࠊ୧⪅ࡀ⤌ྜࢃࡉࡿࡇ࡜࡛ࠊḞ⚄ⓎసࡀⓎ⑕ࡍࡿ࡜⤖ㄽࡋࡓࠋTRM ࣛࢵࢺ࡛ࡣࠊḞ⚄Ⓨసࡣ 2 ࡘࡢ㑇ఏᏊኚ␗ࡢ⤌ࡳྜࢃࡏ࡛Ⓨ⑕ࡍࡿ࢜ࣜࢦࢪ࢙ࢽ
ࢵࢡ⑌ᝈ࡛࠶ࡿࡇ࡜ࡀ♧ࡉࢀࡓࠋࣄࢺࡢḞ⚄࡚ࢇ࠿ࢇࡢⓎ⑕࡟࠾࠸࡚ࡶࠊ࢜ࣜࢦࢪ࢙ࢽࢵࢡࣔࢹࣝࡀᥦၐࡉ
ࢀ࡚࠸ࡿࠋ௒ᚋࠊTRM ⣔࡜ࡣ␗࡞ࡿ㑇ఏⓗ⫼ᬒࢆᣢࡘ⣔⤫࡟ࠊAspa ኚ␗ࠊ
Hcn1 ኚ␗ࢆ஺㓄ࡸ㑇ఏᏊᨵኚᢏ⾡ࢆ⏝࠸࡚ᑟධࡍࡿࡇ࡜࡛ࠊḞ⚄࡚ࢇ࠿
ࢇࡢⓎ⑕࡟ಀࡿ㑇ఏᏊࡢ᥈⣴ࢆ㐍ࡵ࡚࠸ࡃࡇ࡜ࡀ࡛ࡁࡿࠋ

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SM/J ࡜ A/J ⣔⤫ࢆ㉳※࡜ࡍࡿ࣐࢘ࢫయ⣔ⓗ㑇ఏゎᯒ⣔ࢆ⏝࠸ࡓ⾑୰୰ᛶ⬡⫫⃰ᗘࢆつᐃࡍࡿ㑇ఏᏊࡢゎᯒ佹኱㔝Ẹ⏕ 1ࠊᖹ஭ెዉ 2ࠊ๓ᕝᬛᶞ 1ࠊᾏ㔝᫂ᗈ 1ࠊ஭ཎ㑥ኵ 3ࠊᰘ⏣ဴ⚽ 4ࠊ኱㔝ḯྖ 4ࠊᑠᯘ⨾㔛 2ࠊᇼᑿᩥᙪ 2

1 ྡ኱㝔࣭་࣭ᐇ㦂ື≀ࠊ2 ྡ኱㝔࣭⏕࿨㎰࣭ື≀ᰤ㣴ࠊ3 ྡ኱࣭㑇ఏᏊ᪋タࠊ4 ྡ኱㝔࣭་࣭⚄⤒㑇ఏ᝟ሗ  ᡃࠎࡣ࣐࢘ࢫ SM/J ࡜ A/J ⣔⤫ࢆ㉳※࡜ࡋ࡚ࣜࢥࣥࣅࢼࣥࢺ㏆஺⣔ SMXA࣭RI ⣔⤫⩌ ࡸࢥࣥࢯ࣑ࢵࢡ⣔⤫⩌ A/J-NSM ➼ࡢయ⣔ⓗ㑇ఏゎᯒ⣔ࢆᶞ❧ࡋࠊࡑࢀࡽࢆ⏝࠸࡚⢾⬡㉁௦ㅰ࡟㛵ಀࡍࡿ㑇ఏⓗせᅉࡢゎᯒࢆ⾜ࡗ࡚࠸ࡿࠋ௒ᅇ
ࡣࠊ⾑୰୰ᛶ⬡⫫ TG ⃰ᗘࢆつᐃࡍࡿ㑇ఏᏊࡢゎᯒ࡟ࡘ࠸࡚ሗ࿌ࡍࡿࠋ
 ୖ㏙ࡢࢥࣥࢯ࣑ࢵࢡ⣔⤫⩌ࡢ⾑୰ TG ⃰ᗘࢆゎᯒࡋࡓ࡜ࡇࢁࠊA/J-11SM ⣔⤫ࡣ A/J ⣔⤫ࡸ௚ࡢࢥࣥࢯ࣑ࢵࢡ⣔⤫࡟ᑐࡋ࡚᭷ព࡟㧗࠸⾑୰ TG ⃰ᗘࢆ♧ࡋࡓࠋࡑࡇ࡛ࠊChr.11 ࡟Ꮡᅾࡍࡿ⾑୰ TG ⃰ᗘ࡟㛵୚ࡍࡿ㑇ఏᏊࡢ఩⨨࡜ຠᯝࢆ᳨ドࡍࡿࡓࡵ࡟ࠊA/J-11SM ⣔⤫ࢆ㉳※࡜ࡋ࡚ SM/J ⏤᮶ࡢ Chr.11 ࢆ᩿∦໬ࡉࡏࡓࢥࣥࢪ࢙ࢽࢵࢡ⣔⤫⩌ࢆసฟࡋࠊྛ⣔⤫ࡢ⾑୰ TG ⃰ᗘࢆゎᯒࡋࡓࠋࡑ ࡢ ⤖ ᯝ ࠊ ⾑ ୰ TG ⃰ ᗘ ࢆ ᨭ 㓄 ࡍ ࡿ 㑇 ఏ Ꮚ ࡣ D11Mit360 103Mb 㹼
D11Mit48 118Mb ࡟ᏑᅾࡋࠊSM/J ࢔ࣞࣝࡀ⾑୰ TG ⃰ᗘࢆୖ᪼ࡉࡏࡿ஦ࡀุ
᫂ࡋࡓࠋࡇࢀࡽࡢ⤖ᯝࡣࠊ௨๓࡟ᡃࠎࡀ SMXA࣭RI ⣔⤫⩌ࡸ SM/J䤸A/J F2
࡛⾜ࡗࡓ⾑୰ TG ⃰ᗘࡢ QTL ゎᯒࡢ⤖ᯝ࡜ࡼࡃ୍⮴ࡋ࡚࠸ࡓࠋ
୍᪉ࠊᡃࠎࡣ SM/J ࡜ A/J ⣔⤫ࡢࢤࣀ࣒ࡢ࠺ࡕ࢚࢟ࢯࣥ㒊ศࢆᕷ㈍ࡢ࢟ࢵ
ࢺࢆ⏝࠸࡚ຠ⋡ⓗ࡟ᤕᤊࡋࠊࡇࢀࢆḟୡ௦ࢩ࣮ࢣࣥࢧ࣮࡛ゎㄞࡋ࡚୧⣔⤫㛫ࡢ࢚࢟ࢯࣥ㒊ศࡢኚ␗ࢆ⥙⨶ⓗ࡟ゎᯒࡋࡓࠋࡇࡢࢤࣀ࣒࣭ࢹ࣮ࢱ࡜ୖ㏙ࡢ⾑୰ TG ⃰ᗘࡢᏑᅾ㡿ᇦࢆ↷ྜࡉࡏ࡚ೃ⿵㑇ఏᏊࡢᢳฟࢆヨࡳࡿ࡜ྠ᫬࡟ࠊ୧
⣔⤫㛫ࡢࢤࣀ࣒ࡢኚ␗࡟ᇶ࡙࠸ࡓ᪂ࡓ࡞య⣔ⓗ㑇ఏゎᯒ⣔ࡢ฼⏝᪉ἲ࡟ࡘ࠸࡚ࡶ᳨ウࡋ࡚࠸ࡿࠋ

4࣐࢘ࢫࢆ⏝࠸ࡓࢫࢺࣞࣉࢺࢰࢺࢩࣥㄏⓎ⢾ᒀ⑓ឤཷᛶ㑇ఏᏊࡢゎᯒ佹๓ᕝᬛᶞ 1ࠊᑠᯘ⨾㔛 2ࠊᾏ㔝᫂ᗈ 1ࠊ஭ཎ㑥ኵ 3ࠊᰘ⏣ဴ⚽ 4ࠊ኱㔝ḯྖ 4ࠊᇼᑿᩥᙪ 2ࠊ㧗ᶫ㞞ⱥ 5ࠊ኱㔝Ẹ⏕ 1 1 ྡ኱㝔࣭་࣭ᐇ㦂ື≀ࠊ2 ྡ኱㝔࣭⏕࿨㎰࣭ື≀ᰤ㣴ࠊ3 ྡ኱࣭㑇ఏᏊ᪋タࠊ4 ྡ኱㝔࣭་࣭⚄⤒㑇ఏ᝟ሗࠊ5 ྡ኱㝔࣭་࣭⭘⒆⑓⌮  ࢫࢺࣞࣉࢺࢰࢺࢩࣥ STZ ࡣ⮅ȕ ⣽⬊࡟ᑐࡍࡿ≉␗ⓗ࡞⣽⬊ẘᛶࢆ᭷ࡍࡿ

ࡇ࡜࠿ࡽࠊ࣐࢘ࢫ࣭ࣛࢵࢺࡢᐇ㦂ⓗ⢾ᒀ⑓ࣔࢹࣝࡢస〇࡟ỗ⏝ࡉࢀ࡚࠸ࡿࠋ
࣐࢘ࢫ࡛ࡣྂࡃ࠿ࡽ STZ ㄏⓎ⢾ᒀ⑓ࡢឤཷᛶ࡟⣔⤫ᕪࡀᏑᅾࡍࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿࡀࠊࡑࡢཎᅉ㑇ఏᏊࡢゎᯒࡣᑡ࡞ࡃࠊᏑᅾࡀሗ࿌ࡉࢀ࡚࠸ࡿࡢࡣ
H2 Chr.17 , Chr.9, Chr.11 ࡟㝈ࡽࢀ࡚࠸ࡿࠋࡑࡇ࡛ࠊᡃࠎࡣ Chr.11 ࡟⤠ࡾ STZ
ㄏⓎ⢾ᒀ⑓ឤཷᛶ㑇ఏᏊࡢゎᯒࢆ⾜ࡗࡓࠋ A/JࠊSM/JࠊA/J-11SM ⣔⤫࡟ STZ (175mg/kgBW)ࢆ⭡⭍ෆ࡟ᢞ୚ࡋࠊᢞ୚ᚋ
2 ᪥㛫㝸࡛⾑⢾್࡜య㔜ࢆ ᐃࡋࠊᢞ୚ᚋ 14 ᪥┠࡟⾑୰࢖ࣥࢫ್ࣜࣥࢆ ᐃࡋࡓࠋࡑࡢ⤖ᯝࠊA/J ⣔⤫ࡣ௚ࡢ 2 ⣔⤫࡟ᑐࡋ࡚㢧ⴭ࡞㧗⾑⢾ࠊప࢖ࣥࢫࣜ
್ࣥࠊయ㔜ῶᑡࢆ♧ࡋࡓࠋࡋࡓࡀࡗ࡚ࠊA/J ⣔⤫ࡢ Chr.11 ࡟ STZ ㄏⓎ⢾ᒀ⑓ឤཷᛶ㑇ఏᏊࡀᏑᅾࡍࡿࡇ࡜ࡀุ᫂ࡋࡓࠋࡇࡢ㑇ఏᏊࡢ఩⨨ࢆ≉ᐃࡍࡿࡓࡵ
࡟ࠊA/J-11SM ⣔⤫ࢆ㉳※࡜ࡋ࡚ SM/J ⏤᮶ࡢ Chr.11 ࢆ᩿∦໬ࡉࡏࡓࢥࣥࢪ࢙
ࢽࢵࢡ⣔⤫⩌ࢆసฟࡋࠊྛ⣔⤫ࡢ STZ ឤཷᛶࢆゎᯒࡋࡓࠋࡑࡢ⤖ᯝࠊ┠ⓗ࡜ࡍࡿࡍࡿ㑇ఏᏊࡣ D11Mit163 28Mb 㹼D11Mit240 53Mb ࡟Ꮡᅾࡍࡿ஦ࡀุ᫂ࡋࡓࠋ ࡇࡢ㡿ᇦෆ࡟ࡣ STZ ឤཷᛶ࡟㛵୚ࡍࡿ࡜⪃࠼ࡽࢀࡿ᭷ຊ࡞ೃ⿵㑇ఏᏊ
Mpg: N-methylpurine-DNA glycosylase ࡀᏑᅾࡋ࡚࠾ࡾࠊA/J ⣔⤫࡟ࡣࡇࡢ㑇ఏᏊ࡟࢔࣑ࣀ㓟⨨᥮ࢆక࠺ኚ␗ࡀᏑᅾࡋ࡚࠸ࡓࠋ௒ᚋࡣࠊᙜヱ㡿ᇦෆࡢࢧࣈ
࣭ࢥࣥࢪ࢙ࢽࢵࢡ⣔⤫⩌ࢆᵓ⠏ࡋ࡚㑇ఏᏊࡢᏑᅾ㡿ᇦࢆ㝈ᒁࡍࡿ࡜ྠ᫬࡟ࠊ
Mpg ࡢኚ␗࡜ STZ ឤཷᛶ࡜ࡢ㛵ಀᛶ࡟ࡘ࠸᳨࡚ドࡍࡿணᐃ࡛࠶ࡿࠋ5⸆๣⪏ᛶ +S\ORUL ឤᰁ⑕࡟ᑐࡍࡿ⿵᏶௦᭰⒪ἲ‫ۑ‬႐ከṇ࿴ ࠊ㓀ᐙ❶ᘯ ࠊ௒す஧㑻 㸦 ிᗓ་኱㝔࣭ᐇ㦂ື≀ࢭࣥࢱ࣮ࠊ ච␿࣭ᚤ⏕≀㸧࠙⥴ゝࠚ+HOLFREDFWHUS\ORUL +S\ORUL ឤᰁࡀ⫶⅖㸪ᾘ໬ᛶ₽⒆ࡸ⫶⒴ࠊࡉࡽ࡟ࡣ⫶ 0$/7 ࣜࣥࣃ⭘࡞࡝ࡢ⫶࣭༑஧ᣦ⭠⑌ᝈࡢⓎ⑕࡟㛵୚ࡋ࡚࠸ࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗ࡚ࡁࡓࠋ ᖺ  ᭶ࠊSURWRQSXPSLQKLELWRU
33, DPR[LFLOOLQ $03& FODULWKURP\FLQ &$0 ࡟ࡼࡿ +S\ORUL 㝖⳦⒪ἲࡀಖ㝤㐺ᛂ࡜࡞ࡾࠊ✚ᴟⓗ࡟㝖⳦ࡀ⾜ࢃࢀ࡚࠸ࡿࠋࡋ࠿ࡋ㝖⳦⒪ἲࡀ
㐍ࡴ࡟ᚑ࠸㝖⳦⋡ࡀపୗࡋ࡚ࡁ࡚࠾ࡾࠊ⪏ᛶ⳦࡟ᑐࡍࡿ἞⒪ࡀၥ㢟࡜࡞ࡗ࡚࠸ࡿࠋ௒ᅇᡃࠎࡣࠊ⿵᏶௦᭰⒪ἲ࡜ࡋ࡚ࡢ +S\ORUL ࡟ᑐࡍࡿ⏕⸆ࡢຠᯝ࡟ࡘࡁ᳨ウࡋࡓࡢ࡛ሗ࿌ࡍࡿࠋ
࠙ᮦᩱ࡜᪉ἲࠚ ⏕⸆࡜ࡋ࡚㯤⪆ࠊ༙ኟࠊ⏑ⲡࠊேཧࠊ኱㯤ࠊ㯞㯤ࠊⰟ⸆ࠊ᱇⓶ࠊ∻୹⓶ࠊ㯤㐃ࠊⲓ㝞ⵯࠊᰘ⬌ࠊ⸽ⴥࠊ㜵㢼ࠊᮥோࠊⴱ᰿ࠊ୎Ꮚࠊཌᮔ㸦ᰴࢶ࣒ࣛࡼ
ࡾᥦ౪㸧ࢆ౑⏝ࡋࡓ
㸯㸧LQYLWUR ࡟࠾ࡅࡿᢠ⳦ຠᯝࡢ᳨ウ+S\ORULࡢ⳦ᾮ 㸯&)8 ȣO ࡜⏕⸆ࡢ ZDWHUH[WUDFW  PJPOࠊPJPOࠊPJPOࠊPJPOࠊPJPO ȣO ࢆ ΥࠊᚤዲẼୗ 1&2DQG2 ࡛ΰྜࡋ  ᫬㛫᣺┞ࡉࡏࡓᚋࠊ⾑ᾮᐮኳᇵᆅ࡟ ȣO ࢆሬᕸࡋᚤዲẼୗ 1&2DQG2 ࡟  ᪥㛫ᇵ㣴ࡋࠊࢥࣟࢽ࣮ᩘࢆ࢝࢘ࣥࢺࡋࡓࠋ኱㯤ࠊⲓ㝞ⵯࠊ୎Ꮚ࡟ࡘ࠸࡚ࠊ᥋ゐ᫬㛫ࢆ㸮᫬㛫ࠊ㸱᫬㛫ࠊ㸴᫬㛫ࠊ㸯㸰᫬㛫ࠊ㸰㸲᫬㛫࡜ࡋࡓሙྜ
ࡢᢠ⳦ຠᯝࡶ᳨ウࡋࡓࠋ㸰㸧LQYLYR ࡟࠾ࡅࡿᢠ⳦ຠᯝࡢ᳨ウ㸴㐌㱋ࡢ &%/ ࣐࢘ࢫ࡟ +S\ORULࡢ⳦ᾮ 㸯&)8 PO ࢆ⤒ཱྀᢞ୚ࡋឤᰁࡉࡏࠊ┤ᚋࡼࡾ኱㯤ࠊ㯤㐃ࠊⲓ㝞ⵯࠊ୎Ꮚࠊ⏑ⲡࡢ ZDWHUH[WUDFWPJPO ࢆ⮬⏤㣧Ỉࡉࡏࡓࠋ⤒ཱྀឤᰁࡼࡾ㸲㐌ᚋ࡟⫶ࢆ᦬ฟࡋࠊ +
S\ORUL ឤᰁࡢ᭷↓ࢆ +3 㑅ᢥᇵᆅ࡟ࡼࡿᇵ㣴࠾ࡼࡧ 3&5 ἲࢆ⏝࠸᳨࡚ウࡋࡓࠋ࠙⤖ᯝ࡜⪃ᐹࠚ
㸯㸧኱㯤ࠊ㯤㐃ࠊⲓ㝞ⵯࠊ୎Ꮚࠊ⏑ⲡ࡛ࡣࠊ⃰ᗘ PJPOࠊPJPO࡛ LQYLWUR ࡟࠾ࡅࡿ +S\ORUL ࡟ᑐࡍࡿᢠ⳦ຠᯝࢆㄆࡵࡓࠋ኱㯤ࠊⲓ㝞ⵯࠊ୎Ꮚࡣࠊ㸴᫬㛫௨ୖࡢ᥋ゐ࡛ +S\ORUL ࡟ᑐࡍࡿᢠ⳦ຠᯝࢆㄆࡵࡓࠋ
㸰㸧㯤㐃ࠊⲓ㝞ⵯࠊ୎Ꮚࠊ⏑ⲡࢆ LQYLYR ࡛࣐࢘ࢫ࡟ᢞ୚ࡋࡓ⩌࡛ࡣࠊ+
S\ORUL ឤᰁࢆ᭷ព࡟ῶᑡࡉࡏࡿࡇ࡜ࡀ࡛ࡁࡓࠋ ௨ୖࡢ⤖ᯝࠊ㯤㐃ࠊⲓ㝞ⵯࠊ୎Ꮚࠊ⏑ⲡࡣ LQYLYRࠊLQYLWUR ࡛ +S\ORUL࡟ᑐࡍࡿᢠ⳦ຠᯝࡀㄆࡵࡽࢀࠊ+S\ORUL 㝖⳦࡟࠾ࡅࡿ⿵᏶௦᭰⒪ἲ࡜ࡋ࡚ࡢ⏕⸆ࡢྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ

6࢖ࣥࢱ࣮ࣟ࢖࢟ࣥ㸴ࡣ࢝࣊࢟ࢩ࢔ࡢ⑓ែ⥔ᣢస⏝ࡔࡅ࡛࡞ࡃࠊ࢝࣊࢟ࢩ࢔ㄏᑟ⬟ࢆ௜୚ࡍࡿᅉᏊ࡜ࡋ࡚స⏝ࡍࡿ‫ۑ‬㕥ᮌ ᪼ࠊ㰻⸨ ᾈ඘ 㸦୕㔜኱࣭⏕࿨⛉Ꮫ◊✲ᨭ᥼ࢭ࣭ື≀ᶵ⬟ࢤࣀ࣑ࢡࢫ㸧ࡀࢇᛶᝏᾮ㉁㸦௨ୗࠊ࢝࣊࢟ࢩ࢔㸧ࡣࠊ㣗ḧῶ㏥࡜➽⫗ࡸ⬡⫫⤌⧊ࡢ⃭ῶ㸦ࡿ࠸ࡑ࠺㸧ࢆ≉ᚩ࡜ࡍࡿ⑓ែ࡛࠶ࡿࠋᝈ⪅ࡢ⏕άࡢ㉁㸦QOL㸧ࢆపୗࡉࡏࠊᵝࠎ࡞ᢠࡀࢇ⒪ἲࢆᅔ㞴࡜ࡍࡿ⤖ᯝࠊࡀࢇṚࡢ㸰㸮㸣ࡢṚᅉ࡜࡞ࡗ࡚࠸ࡿࠋࡀࢇ◊✲࡟࠾࠸࡚ࠊ࢝࣊࢟ࢩ࢔ࡢᇶ
♏◊✲ࡣࠊࡀࢇ⣽⬊ࡢቑṪ␗ᖖࡸᾐ₶࣭㌿⛣ࡢ◊✲࡜ẚ㍑ࡋࠊⓎᒎ㏵ୖ࡛࠶ࡿࠋ࡝ࡢࡼ࠺
࡞ศᏊᶵᗎ࡛ࡀࢇ⣽⬊ࡀᐟ୺࡟࢝࣊࢟ࢩ࢔ࢆⓎ⑕ࡉࡏࡿᛶ㉁ࢆ⋓ᚓࡍࡿࡢ࠿࡟ࡘ࠸࡚ࡣ
࡯࡜ࢇ࡝୙࡛᫂࠶ࡿࠋࢃࢀࢃࢀࡢᶞ❧ࡋࡓ㑇ఏᏊᨵኚᆺ⮬ᐙࡀࢇࣔࢹࣝື≀ࡣࠊp53 㑇ఏᏊᶵ⬟Ḟᦆ࡜άᛶᆺ ras 㑇ఏᏊࡢࢩࢼࢪ࣮࡟ࡼࡗ࡚ࠊ100%ࡢ⋡࡛ᶓ⣠➽⫗⭘ࢆᢸ࠸ࠊࡍ
࡭࡚ࡢಶయࡀ࢝࣊࢟ࢩ࢔ࡢ⑓ែࢆ⤒㐣ࡋ⤊ᮎࢆ㏄࠼ࡿࠋࢃࢀࢃࢀࡣࠊࡇࡢ⫗⭘࠿ࡽࠊ⭘⒆ሢࢆᙧᡂࡋᐟ୺࡟࢝࣊࢟ࢩ࢔ࢆⓎ⑕ࡉࡏࡿ⣽⬊ᰴ㸦RMS3㸧࡜⭘⒆ሢࡣᙧᡂࡍࡿࡀᐟ୺࡟
࢝࣊࢟ࢩ࢔ࢆⓎ⑕ࡉࡏ࡞࠸⣽⬊ᰴ㸦RMS6㸧ࢆᶞ❧ࡋࡓࠋ௒ᅇࠊRMS3 ⏤᮶⭘⒆࡟Ⓨ⌧ஹ㐍ࡀㄆࡵࡽࢀࡿ࢖ࣥࢱ࣮ࣟ࢖࢟ࣥ㸴㸦IL-6㸧࡟ࡘ࠸࡚ࠊࢃࢀࢃࢀࡢࣔࢹࣝ࡟࠾ࡅࡿ࢝࣊࢟
ࢩ࢔ࡢ⑓ែࡢㄏᑟ࡜⥔ᣢࡢほⅬ࠿ࡽࡑࡢస⏝ࢆㄪ࡭ࡓࠋ
ࠕ᪉ἲࠊ⤖ᯝࠊ⪃ᐹࠖ࢝࣊࢟ࢩ࢔ࡢ⑓ែࡢㄏᑟ࣭⥔ᣢ࡜ IL-6 Ⓨ⌧ஹ㐍ࡢᅉᯝ㛵ಀࢆㄪ࡭ࡿࡓࡵࠊ࢝࣊࢟ࢩ࢔
ㄏᑟ⬟ࢆᣢࡓ࡞࠸㸰✀㢮ࡢ⣽⬊ᰴࠊRMS6 ⣽⬊ᰴ࡜ᝏᛶ࣓ࣛࣀ࣮࣐⏤᮶ B16F10 ࡟㑇ఏᏊࢆᑟධࡋ࡚ࠊ
IL-6 ࢆᵓᡂⓗ࡟Ⓨ⌧ࡉࡏࡓ⣽⬊ᰴ RMS6-IL6 ࡜ B16F10-IL6 ࢆᶞ❧ࡋࡓࠋ
ྛ 100 ୓ಶࢆ⛣᳜ࡉࢀࡓື≀ࡣࠊRMS3 ࢆ⛣᳜ࡉࢀࡓື≀࡜ྠᵝ࡟ࠊ⣙ 14 ᪥࡛࠸ࡎࢀࡶ
㔜⠜࡞࢝࣊࢟ࢩ࢔ࢆⓎ⑕ࡋࡓࠋࡇࡢ᫬ࠊ⾑Ύ IL-6 ⃰ᗘࡣࠊࡑࢀࡒࢀ⣙ 800pg/mlࠊ⣙8400pg/ml ࡜ṇᖖ⾑Ύ࡜ẚ࡭᭷ព࡟㧗್ࢆ♧ࡋࡓࠋ࢝࣊࢟ࢩ࢔Ⓨ⑕ᚋࠊᢠ IL-6 ཷᐜయᢠయࡢᢞ୚࡟ࡼࡗ࡚ࠊB16F10-IL6 ⏤᮶⭘⒆࡟ࡼࡿ࢝࣊࢟ࢩ࢔⑕≧ࡣᢚไࡉࢀࡓࡀࠊࡼࡾ⾑Ύ IL-6 ⃰ᗘࡀప್࡛࠶ࡿ࡟ࡶ࠿࠿ࢃࡽࡎ RMS6-IL6 ⏤᮶⭘⒆࡟ࡼࡿ࢝࣊࢟ࢩ࢔ࡣᢚไࡉ
ࢀ࡞࠿ࡗࡓࠋࡋ࠿ࡋ࡞ࡀࡽࠊRMS6-IL6 ⏤᮶⭘⒆ࢆᢸ࠺ື≀࡛ࡣࠊᢠ IL-6 ཷᐜయᢠయࢆ
⭘⒆ᙧᡂࡢึᮇ࠿ࡽᢞ୚ࡍࡿࡇ࡜࡟ࡼࡗ࡚ࠊⓎ⑕ࣉࣟࢭࢫࡀ㐜ᘏࡋࠊᢠ IL-6 ཷᐜయᢠయ
ᢞ୚ࡢ᭷ព࡞἞⒪ຠᯝࢆㄆࡵࡓࠋ௨ୖࡢ⤖ᯝࠊࡇࢀࡲ࡛♧ࡉࢀࡓ IL-6 ࡢࡀࢇ࢝࣊࢟ࢩ࢔
⑓ែࢆ⥔ᣢࡍࡿస⏝ࡢ࡯࠿ࠊ᪂ࡓ࡟ࠊIL-6 ࡀࡀࢇ⣽⬊࡟࢝࣊࢟ࢩ࣮ㄏᑟ⬟ࢆ௜୚ࡍࡿస
⏝ࡶ࠶ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ

7⮬↛ච␿ᅉᏊ࣏ࣜ࢝ࣜࣥ㸰ࡣ⣽⬊⭷ࣜࣥ⬡㉁ࣇ࢛ࢫࣇ࢓ࢳࢪ࢚ࣝࢱࣀ
࣮ࣝ࢔࣑ࣥ࡟⤖ྜࡋࠊ㞤⏕Ṫ㊰ෆ࡛⢭Ꮚᡂ⇍ࢆಁ㐍ࡍࡿΏ㑔 ோ⨾ 1ࠊ➉ᑿ ㏱ 2ࠊᮾᇛ ༤㞞 3ࠊ୰₲ ┤ᕫ 2ࠊTak W. Mak4ࠊ
䕿㏆⸨ ⋞㸯(ி኱࣭෌⏕◊㸯ࠊ⇃኱࣭⏕࿨㈨※㸰ࠊ㜰኱㝔࣭་࣭ศ⏕໬㸱ࠊࢺࣟࣥࢺ
኱㸲)့ஙື≀ࡢ⢭Ꮚࡣࠊẁ㝵ⓗ࡞ᡂ⇍㐣⛬ࢆ⤒࡚᏶඲࡞ཷ⢭⬟ࢆ⋓ᚓࡍࡿࡀࠊ㞤⏕Ṫჾෆ࡛ࡢヲ⣽࡞ศᏊ࣓࢝ࢽࢬ࣒ࡣࢃ࠿ࡗ࡚࠸࡞࠸ࠋ௒ᅇᡃࠎ
ࡣࠊ㞤⏕Ṫ㊰ෆ࡛⢭Ꮚᡂ⇍ࢆㄏᑟࡍࡿᅉᏊ࡜ࡋ࡚࣏ࣜ࢝ࣜࣥ㸰(Lipocalin2, Lcn2) ࢆぢฟࡋࡓࠋ㔝⏕ᆺ࣐࢘ࢫࡢ༸⟶࡟㐟㉮ࡋࡓ⢭Ꮚ࡛ࡣࠊ⣽⬊⭷ࣛࣇࢺࡢ෌ᵓᡂࡸ GPI ࢔࣮ࣥ࢝ᆺࢱࣥࣃࢡ㉁ࡢ㐟㞳ࡀ㉳ࡇ
ࡿࡀࠊLcn2 ࣀࢵࢡ࢔࢘ࢺ࣐࢘ࢫ࡛ࡣࡑࡢኚ໬ࡀ㢧ⴭ࡟ῶᑡࡋ࡚࠸ࡓࠋ
ࡲࡓࠊLcn2 ࡣ⣽⬊⭷ࢆᵓᡂࡍࡿ୺せࣜࣥ⬡㉁ࡢࡦ࡜ࡘ࡛࠶ࡿࣇ࢛ࢫࣇ
࢓ࢳࢪ࢚ࣝࢱࣀ࣮ࣝ࢔࣑ࣥ (PE) ࡜┤᥋⤖ྜࡋࠊࡉࡽ࡟ࢥࣞࢫࢸ࣮ࣟ
ࣝ㐟㞳ࡸ௒ᅇぢฟࡋࡓ PE 㞟✚㡿ᇦ (PE-enriched domain, PED)ࡢゎᾘࢆᘬࡁ㉳ࡇࡋࠊ ⢭Ꮚࡢ࢟ࣕࣃࢩࢸ࣮ࢩࣙࣥࢆಁ㐍ࡋࡓࠋࡇࢀࡽࡢࡇ
࡜࠿ࡽࠊ௒ᅇᡃࠎࡣࠊ့ஙື≀࡟ࡣ࢔ࣝࣈ࣑ࣥࢆ௓ࡍࡿ࢟ࣕࣃࢩࢸ࣮
ࢩࣙࣥ⤒㊰࡟ຍ࠼࡚ࠊࡶ࠺୍ࡘࡢ࢟ࣕࣃࢩࢸ࣮ࢩ࣓ࣙࣥ࢝ࢽࢬ࣒ࢆࡶࡘࡇ࡜ࢆぢฟࡋࡓࠋ

8ႚ↮᭚㟢ࣛࢵࢺ࡟ᑐࡍࡿศᏊ㙐࢔࣑ࣀ㓟ࡢຠᯝ‫ஂۑ‬ಖ ⸅㸯ࠊ཭⏣ᜏ୍㸰ࠊྜྷᕝ㞞๎㸰ࠊඣᒣ⣖Ꮚ㸰ࠊᒣᮏᆂྐ㸰ࠊᮌᮧ ᘯ㸰ዉⰋ་኱࣭㸯ື≀ᐇ㦂᪋タ㸰➨஧ෆ⛉  ៏ᛶ㛢ሰᛶ⫵⑌ᝈ㸦&23'㸧ࡣႚ↮ࢆ୺ᅉ࡜ࡋ࡚Ⓨ⑕ࡍࡿ඲㌟ᛶ⑌ᝈ࡛࠶ࡾࠊ
ᰤ㣴㞀ᐖࡣ &23' ࡢ㔜せ࡞͆V\VWHPLFHIIHFW͇࡛࠶ࡿ࡜࡜ࡶ࡟ࠊⓎ⑕ࡸ㐍ᒎ࡟ࡶ㛵୚ࡍࡿࡇ࡜ࡀ♧၀ࡉࢀ࡚࠸ࡿࠋศᏊ㙐࢔࣑ࣀ㓟㸦%&$$㸧ࡣ౵く᫬ࡸ㐠ື᫬࡟➽⫗࡛ࡢ㟂せࡀ㧗ࡲࡗ࡚࠾ࡾࠊ&23' ࡛ࡣ⾑₢୰ %&$$ ⃰ᗘࡢపୗࡀ
ㄆࡵࡽࢀ࡚࠸ࡿࠋ௒ᅇࠊᐇ㦂ື≀ࢆ⏝࠸࡚➽⫗ෆ࡞ࡽࡧ࡟⾑₢୰ࡢ %&$$ ⃰ᗘ࡟ᑐࡍࡿႚ↮᭚㟢ࡢᙳ㡪ࢆ᳨ウࡋࡓࠋ࠙ᮦᩱ࡜᪉ἲࠚ 㐌㱋ࡢ㞝 :LVWDU.\RWR :.<,]P ࡟ᑐࡋ࡚ 0,36 ♫〇ႚ↮᭚㟢⿦⨨ࢆ⏝࠸࡚ࠊႚ↮᭚㟢 ࣁ࢖ࣛ࢖ࢺp ᮏ ศࠊ ᅇ᪥ ࢆ㸳ᅇ㐌 ᭶࠿ࡽ㔠᭙᪥ ࠊ㐌㛫⾜ࡗࡓࠋႚ↮᭚㟢ᮇ㛫୰ࠊ$,1*㸦㏻ᖖ㣗㸧࠶ࡿ࠸ࡣ %&$$ ࢆྵࡴ $,1*
㸦%&$$ ௜ຍ㣗㸧ࢆ୙᩿⤥㣵࡜ࡋࠊ㠀ႚ↮㸭㏻ᖖ㣗⩌ࠊ㠀ႚ↮㸭%&$$ ௜ຍ㣗⩌ࠊႚ↮㸭㏻ᖖ㣗⩌࡜ႚ↮㸭%&$$ ௜ຍ㣗⩌᳨࡛ウࡋࡓࠋẖ㐌ᅵ᭙᪥࡟య㔜ࢆ ᐃࡋࠊႚ↮᭚㟢㸲㐌┠࡟ᦤ㣵㔞ࢆィ㔞ࡋࡓࠋ᭱⤊ႚ↮᭚㟢ᚋࠊ⾑ᾮ࡞ࡽࡧ࡟ࣄ࣓ࣛ➽ࢆ᥇ྲྀࡋࠊ⾑₢୰࡞ࡽࡧ࡟➽⫗ෆ࢔࣑ࣀ㓟ࠊYDOLQHOHXFLQLVROHXFLQH ⃰ᗘࢆ +3/& ἲ࡛ ᐃࡋࡓࠋ
࠙⤖ᯝ࡜⪃ᐹࠚ
య㔜࡜ᦤ㣵㔞ࡣࠊ㠀ႚ↮⩌࡟ẚࡋ࡚ႚ↮⩌࡛᭷ព࡟పୗࡋࡓࠋႚ↮᭚㟢࡟ࡼࡾ➽⫗㔜㔞ࡣ᭷ព࡟ῶᑡࡋࡓࡀࠊࡇࢀ࡟ᑐࡋ࡚ %&$$ ௜ຍ㣗ࡢᦤྲྀࡣ➽⫗㔜㔞ࢆ㠀ႚ↮㸭㏻ᖖ㣗⩌࡜ྠ⛬ᗘ࡟ࡲ࡛ᅇ᚟ࡉࡏࡓࠋࡇࡢഴྥࡣ➽⫗୰ %&$$ ⃰ᗘ࡞ࡽࡧ࡟ YDOLQH ⃰ᗘࡢኚ໬࡟࠾࠸࡚ࡶྠᵝ࡛࠶ࡗࡓࠋႚ↮᭚㟢࡟ࡼࡾ⾑₢୰%&$$ ⃰ᗘࡣῶᑡࡋࠊ
ࡲࡓ YDOLQH ⃰ᗘࡣ᭷ព࡟ῶᑡࡋࠊOHXFLQ ⃰ᗘ࡜ LVROHXFLQH⃰ᗘࡶῶᑡࡋࡓࠋࡇࢀࡽ࡟ᑐࡋ࡚ %&$$ ௜ຍ㣗ࡢᦤྲྀࡣࠊ⾑₢୰ %&$$ ⃰ᗘ࡞ࡽࡧ࡟ YDOLQH ⃰ᗘ OHXFLQ ⃰ᗘ࡜ LVROHXFLQH ⃰ᗘࢆ᭷ព࡟ቑຍࡉࡏࡓࠋ㠀ႚ↮⩌࡟࠾࠸࡚ࡣࠊ%&$$ ௜ຍ㣗ࡢᦤྲྀ࡟ࡼࡾ➽⫗㔜㔞࡞ࡽࡧ࡟⾑₢୰ %&$$ ⃰ᗘࠊ
YDOLQH ⃰ᗘ OHXFLQ ⃰ᗘ࡜ LVROHXFLQH ⃰ᗘࡀ᭷ព࡟ቑຍࡋࡓࠋ
௨ୖࡢ⤖ᯝࠊ%&$$ ⿵⤥ࡣႚ↮࡟ࡼࡿ➽⫗㔜㔞࠾ࡼࡧ⾑₢୰࡞ࡽࡧ࡟➽⫗୰ࡢ%&$$ ⃰ᗘࡢῶᑡ࡟ᑐࡋ࡚᭷ຠ࡛࠶ࡾࠊ&23' ࡢేᏑ⑕࡛࠶ࡿ㦵᱁➽ᶵ⬟㞀ᐖࢆ
㍍ῶࡍࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ9
ZFDM ࣛࢵࢺ㸸᪂つ⫧‶࣭⢾ᒀ⑓ࣔࢹࣝࡢ☜❧‫ۑ‬ᶓ஭఑ⱥ 1,2ࠊᫍ㔝㞞⾜ 3ࠊ᪥㧗ᚿಖ⨾ 2ࠊΎ㔝 㐍 2
㸦1 ⚄ᡞ኱㝔࣭་࣭ศᏊ௦ㅰ་Ꮫࠊ2 ⣽⬊ศᏊ་Ꮫࠊ3 ᫍ㔝ヨ㦂ື≀㣫⫱ᡤ㸧࠙┠ⓗࠚZucker fatty㸦ZF㸧ࣛࢵࢺࡣࣞࣉࢳࣥཷᐜయ㸦Lepr㸧࡟✺↛ኚ␗㸦fatty, fa㸧ࢆ᭷ࡋ⫧‶ࢆⓎ⑕ࡍࡿࠋZucker diabetic fatty㸦ZDF㸧
ࣛࢵࢺࡣ ZF ࣛࢵࢺ࠿ࡽศ㞳࣭☜❧ࡉࢀࡓ⫧‶࡜⢾ᒀ⑓ࢆⓎ⑕ࡍࡿ⣔⤫࡛࠶ࡾࠊ⫧‶࣭⢾ᒀ⑓◊✲࡟ᗈࡃ⏝࠸ࡽࢀ࡚࠸ࡿࠋ௒ᅇࠊZF ࣛࢵࢺࢆ㉳※࡜ࡋ࡚᪂ࡓ࡟⫧‶࡜⢾ᒀ⑓ࢆⓎ⑕ࡍࡿ⣔⤫ࡀศ㞳࣭☜❧ࡉࢀࡓࡢ࡛ሗ࿌ࡍࡿࠋ
࠙᪉ἲࠚ2008 ᖺ࡟ᫍ㔝ヨ㦂ື≀㣫⫱ᡤ࡟࠾࠸࡚⥔ᣢࡉࢀ࡚࠸ࡓ ZF ࣛࢵࢺࡢࢥࣟࢽ࣮࡟⢾ᒀ⑓ࢆⓎ⑕ࡍࡿࣛࢵࢺࡀⓎぢࡉࢀࡓࠋfa/fa ࣍ࣔࡢ࢜ࢫࡀ⦾Ṫᛶࢆ᭷ࡋ࡚࠸ࡓࡇ࡜࠿ࡽࠊ⾑⢾್ࢆ㑅ᢤᇶ‽࡜ࡋ࡚ fa/+࣊
ࢸࣟࡢ࣓ࢫ࡜ࡢ㑅ᢤ஺㓄ࡀ⧞ࡾ㏉ࡋ⾜ࢃࢀࡓࠋࡑࡢ⤖ᯝࠊ20 㐌㱋๓ᚋ
࡛ fa/fa ࣍ࣔࡢ࢜ࢫࡢ࡯ࡰ඲౛ࡀ⢾ᒀ⑓ࢆⓎ⑕ࡍࡿࢥࣟࢽ࣮ࡀ☜❧ࡉࢀ ࠊ Zucker fatty diabetes mellitus 㸦ZFDM㸧ࣛࢵࢺ㸦ṇᘧྡ⛠
Hos:ZFDM-Leprfa㸧࡜ྡ௜ࡅࡽࢀࡓࠋ௒ᅇࡣ ZFDM ⣔⤫ࡢ fa/fa ࣍ࣔ࡜ᑐ↷ࡢ fa/+࣊ࢸࣟࡢ࢜ࢫ࡟ࡘ࠸࡚ 5 㐌㱋࠿ࡽ 22 㐌㱋ࡲ࡛య㔜࣭⾑⢾್ࢆ ᐃࡋࠊ⮅⮚ࡢ⤌⧊Ꮫⓗゎᯒࢆ⾜ࡗࡓࠋ࠙⤖ᯝࠚfa/fa ࣍ࣔࡣ fa/+࣊ࢸࣟ࡜ẚ㍑ࡋ࡚ 6 㐌㱋௨㝆య㔜ࡀ᭷ព࡟኱
ࡁࡃࠊ8 㐌㱋௨㝆ࡣ⾑⢾್ࡀ᭷ព࡟㧗࠿ࡗࡓࠋfa/fa ࣍ࣔࡣ 10 㐌㱋࠿ࡽ 21 㐌㱋ࡲ࡛ࡢ㛫࡟඲౛ࡀ⢾ᒀ⑓㸦⾑⢾್ 300mg/dl ௨ୖ㸧ࢆⓎ⑕ࡋࡓࠋfa/fa ࣍ࣔࡢ⮅⮚࡟ࡣࠊ⮅ᓥᵓ㐀ࡢᾘኻࠊ⥺⥔໬ࠊ⅖⑕⣽⬊ᾐ₶࡞࡝ࡢ⑓⌮⤌⧊Ꮫⓗኚ໬ࡀㄆࡵࡽࢀࡓࠋ
࠙⤖ㄒࠚ᪂ࡓ࡟☜❧ࡉࢀࡓ ZFDM ࣛࢵࢺࡣࠊfa/fa ࣍ࣔࡢ࢜ࢫࡀ⦾Ṫᛶࢆ᭷ࡍࡿࡇ࡜࠿ࡽࠊ㧗࠸⏕⏘ຠ⋡ࢆᐇ⌧ࡋࡓ⫧‶࣭⢾ᒀ⑓ࣔࢹࣝ࡜
ࡋ࡚᭷⏝࡛࠶ࡿࠋ

10࣑࢚ࣜࣥ␗ᖖ࣑࣮ࣗࢱࣥࢺ dmy ࣛࢵࢺࡢ࣑࢚ࣜࣥᔂቯ࡟࠾ࡅࡿ⚄⤒⣽⬊ࡢ⑓ែ‫ۑ‬㛗㇂ᕝ ඃᏊ 1ࠊᒣᡭ ୔⮳ 1ࠊ஭⃝ Ṋྐ 1ࠊᗜᮏ 㧗ᚿ 2ࠊⱂᕝ ᛅኵ 2ࠊ᱓ᮧ ඘ 1
1 ኱㜰ᗓ❧኱Ꮫࠊ2 ி኱㝔࣭་࣭ື≀ᐇ㦂᪋タ㸧

demyelination (dmy) ࣛࢵࢺࡣࠊᚋ⫥ࡢ㐠ືኻㄪࢆ≉ᚩ࡜ࡍࡿ⮬↛Ⓨ⑕࣑࣮ࣗࢱࣥࢺ
࡛ࠊ 7 㐌㱋㡭ࡼࡾ୰ᯡ⚄⤒⣔ࡢⓑ㉁࡟࠾࠸࡚ᛴ㏿࡞࣑࢚ࣜࣥᔂቯࡀ㐍⾜ࡍࡿࠋࡑࡢཎᅉ
㑇ఏᏊࡣ࣑ࢺࢥࣥࢻࣜ࢔ෆࡢ࣐ࢢࢿࢩ࣒ὶධ࡟㛵୚ࡍࡿ Mrs2 㑇ఏᏊ࡛࠶ࡿࡇ࡜ࡀ᫂ࡽ
࠿࡟ࡉࢀ࡚࠸ࡿࠋMRS2 ࡣ୺࡟୰ᯡ⚄⤒⣔ࡢ⚄⤒⣽⬊࡟ศᕸࡋ࡚࠾ࡾࠊ⚄⤒⣽⬊ࡢ࣑ࢺࢥࣥࢻࣜ࢔␗ᖖࡀ࡝ࡢࡼ࠺࡟࣑࢚ࣜࣥᙧᡂ⣽⬊࡛࠶ࡿ࢜ࣜࢦࢹࣥࢻࣟࢧ࢖ࢺࢆ㞀ᐖࡋࠊ࣑࢚ࣜࣥᔂቯ࡟⮳ࡿ࠿࡟ࡘ࠸࡚ࡣ୙᫂࡞Ⅼࡀከ࠸ࠋᮏ◊✲ࡣࠊdmy ࣛࢵࢺࡢ⑓⌮Ⓨ⏕ࢆ᫂ࡽ࠿࡟ࡍࡿ┠ⓗ࡛ࠊ≉࡟⚄⤒⣽⬊ࡢ⑓ែ࡟ὀ┠ࡋ࡚ゎᯒࡋࡓࠋ࠙ᮦᩱ࡜᪉ἲࠚ
 6ࠊ7ࠊ8 㐌㱋ࡢ dmy ࣛࢵࢺࡢ࣍ࣔᆺ࠾ࡼࡧᑐ↷ࣛࢵࢺ(㔝⏕ᆺ)ࡢ⬨㧊ࢆ⭡⣴࣭⅊ⓑ㉁࣭
⫼⣴࡟ࢃࡅ࡚᥇ᮦࡋࠊձReal-time PCR ἲ࡟ࡼࡾࠊྛ㒊఩࡟࠾ࡅࡿ࢜ࣜࢦࢹࣥࢻࣟࢧ
࢖ࢺࡢศ໬࡟㛵ࢃࡿ㌿෗ᅉᏊࡢ mRNA Ⓨ⌧㔞ࢆ ᐃࡋࡓࠋղᢠ࣑ࢺࢥࣥࢻࣜ࢔ COXϫᢠయࢆ⏝࠸ࡓච␿⤌⧊໬Ꮫゎᯒ࡟ࡼࡾࠊ⚄⤒⣽⬊࡟࠾ࡅࡿ࣑ࢺࢥࣥࢻࣜ࢔ࡢኚ໬ࢆẚ㍑
ࡋࡓࠋճᢠ Golgi ᢠయࠊᢠࣘࣅ࢟ࢳࣥᢠయࢆ⏝࠸ࡓච␿⤌⧊໬Ꮫゎᯒ࡜ࠊPLP ࡟ᑐࡍࡿin situ hybridization ἲ࡟ࡼࡗ࡚ࠊ⭡⣴ࡢ࢜ࣜࢦࢹࣥࢻࣟࢧ࢖ࢺࡢᰁⰍᛶࢆẚ㍑ࡋࡓࠋ
࠙⤖ᯝ࡜⪃ᐹࠚ
ձ࢜ࣜࢦࢹࣥࢻࣟࢧ࢖ࢺࡢศ໬࡟㛵ࢃࡿ㌿෗ᅉᏊ㸦PDGFRDࠊOlig2ࠊNkx2.2㸧ࡣ 6ࠊ
7ࠊ8 㐌㱋ࡢ࣍ࣔᆺࣛࢵࢺ࡟࠾࠸࡚ࠊ≉࡟⅊ⓑ㉁࡛ῶᑡࡋࡓࠋղ7ࠊ8 㐌㱋ࡢ࣍ࣔᆺࣛࢵ
ࢺ࡟࠾࠸࡚ࠊ⚄⤒⣽⬊࡟࠾ࡅࡿ࣑ࢺࢥࣥࢻࣜ࢔ࡢච␿ᰁⰍᛶࡀపୗࡋࡓࠋճ࣍ࣔᆺࣛࢵ
ࢺࡢ⭡⣴࡟࠾࠸࡚ࡳࡽࢀࡿ⭘኱ࡋࡓ࢜ࣜࢦࢹࣥࢻࣟࢧ࢖ࢺࡢ⣽⬊㉁ෆ࡟ Golgi ࡢච␿ᰁⰍᛶࡢቑᙉࡀࡳࡽࢀࠊࡲࡓࠊࣘࣅ࢟ࢳࣥࡢච␿ᰁⰍᛶࡶቑຍࡋࡓࠋ
௨ୖࡼࡾࠊ⚄⤒⣽⬊࡟࠾ࡅࡿ࣑ࢺࢥࣥࢻࣜ࢔ࡢ␗ᖖ࡟క࠸ࠊ࢜ࣜࢦࢹࣥࢻࣟࢧ࢖ࢺࡢ
ศ໬࣭ᡂ⇍࣭ᶵ⬟␗ᖖࡀ⏕ࡌࠊࡑࡢ⤖ᯝࠊ࣑࢚ࣜࣥᙧᡂ㞀ᐖࡀ࠾ࡇࡗ࡚࠸ࡿྍ⬟ᛶࡀ♧
ࡉࢀࡓࠋ

11

㑇ఏᏊᨵኚ⫵ࡀࢇࣔࢹ࣐ࣝ࢘ࢫࢆ⏝࠸ࡓⓎ⒴ឤཷᛶ㛵㐃㑇ఏᏊ᥈⣴ࢩࢫࢸ࣒ࡢస〇࡜ゎᯒ‫ۑ‬㰻⸨ ᾈ඘ࠊ㕥ᮌ ᪼ 㸦୕㔜኱࣭⏕࿨⛉Ꮫ◊✲ᨭ᥼ࢭ࣭ື≀ᶵ⬟ࢤࣀ࣑ࢡࢫ㸧㸦⫼ᬒ㸧ࣄࢺ⫵⒴  ࡛ࡣࠊ㑇ఏᏊ࡟ᜏᖖⓗάᛶᆺ .UDV㸦⒴ᆺ .UDV㸧ኚ␗ࡀ㧗⋡࡟᳨ฟࡉࢀࡿࠋᡃࠎࡣࠊ&UH ࢱࣥࣃࢡ࡟ࡼࡿ⤌ࡳ᥮࠼࡛ࠊ௵ពࡢ᫬ᮇࠊ
⣽⬊࡛⒴ᆺ .5DV Ⓨ⌧ࢆㄏᑟ࡛ࡁࡿ IOR[⒴ᆺ .5DV ࣐࢘ࢫࢆసᡂࡋࡓࠋ&UHⓎ⌧࢔ࢹࣀ࢘࢖ࣝࢫ࣋ࢡࢱ࣮ࢆ⏝࠸ࡓ⫵⬊ୖ⓶⣽⬊≉␗ⓗ࡞⒴ᆺ .5DV Ⓨ⌧
࡟ࡼࡾࠊࣄࢺࡢ⫵⬊ୖ⓶㸰ᆺᵝࡢ⭢⒴Ⓨ⑕ࢆㄏᑟ࡛ࡁࠊ⮬↛Ⓨ⒴㸦ࣄࢺ࡛ࡢⓎ⒴㸧࡟࠾ࡅࡿࠕ⒴ᆺ .5DV ኚ␗࠿ࡽ⭘⒆ฟ⌧ࡲ࡛ࡢ⫵⒴Ⓨ⑕㐣⛬ࠖࢆᐇ㦂ⓗ࡟෌⌧࡛ࡁࡿࠋ␗࡞ࡿ㏆஺⣔࣐࢘ࢫࡢ㑇ఏᏊ⫼ᬒࢆࡶࡕࠊ␗࡞ࡿ⒴ᆺ .UDV ㄏᑟ⫵Ⓨ⒴ឤཷᛶࢆࡶࡘ࣐࢘ࢫస〇࡟ࡼࡾࠊ⒴ᆺ .UDV ኚ␗ࢆཎᅉ࡜ࡍࡿ⫵Ⓨ⒴ࡢ
ᢚไࠊಁ㐍ᅉᏊࢆ⏕య࡛ࣞ࣋ࣝ᥈⣴ࡍࡿࢩࢫࢸ࣒ࡢస〇ࢆヨࡳࡓࠋ㸦⤖ᯝ࡜⪃ᐹ㸧$- ⣔⤫࠾ࡼࡧ &%/- % ⣔⤫ࡢ㑇ఏᏊ⫼ᬒࢆࡶࡗࡓ IOR[⒴ᆺ .UDV ࣐࢘ࢫࢆస〇ࡋࠊ⫵Ⓨ⒴ឤཷᛶࢆẚ㍑᳨ウࡋࡓࠋ⒴ᆺ .UDV Ⓨ⌧࡟ࡼࡿⓎ⒴ᐇ㦂ࡢ⤖ᯝࠊ% ⣔⤫㸦142 ± 59.5㸧ࡀ $- ⣔⤫ 11.4 ± 14.8 ࡼࡾ⫵Ⓨ⒴㧗ឤཷᛶ࡛࠶ࡗࡓ㸦P<0.05㸧ࠋࡇࡢ㸰⣔⤫࡟࠾࠸࡚⒴ᆺ .UDV Ⓨ⌧ㄏᑟ࡟⏝࠸࡚࠸ࡿ࢔ࢹࣀ࢘࢖ࣝࢫឤᰁຠ⋡࡟ᕪࡣ᳨ฟࡉࢀࡎࠊ㸰⣔⤫ࡢ㑇ఏᏊ⫼ᬒࡢ㐪࠸࡟ࡣ⒴ᆺ .5DV ࡟ࡼࡾㄏᑟࡉࢀࡿ⫵Ⓨ⒴ឤཷᛶࢆㄪ⠇ࡍࡿᅉᏊࡀྵࡲࢀ࡚࠸
ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋࡲࡎࠊṇᖖ࡞ .5DV 㑇ఏᏊ࡟ࡼࡿ⫵Ⓨ⒴ᢚไຠᯝࡀሗ࿌
ࡉࢀ࡚࠸ࡿࡓࡵ᳨ウࢆ⾜ࡗࡓࠋ ⣔⤫ࡢ⫵࡟࠾ࡅࡿෆᅾᛶ .5DV 㑇ఏᏊⓎ⌧㔞ࢆ 573&5 ἲ࡟ࡼࡾẚ㍑ࡋࡓ⤖ᯝࠊ㑇ఏᏊⓎ⌧㔞ࡀ㧗ឤཷᛶࢆ♧ࡍ % ⣔⤫ࡣ
㸦0.73 ± 0.087㸧ࠊ$- ⣔⤫ 1.00 ± 0.057)ࡼࡾ᭷ព࡟పࡃ 3  ࠊⓎ⒴ឤཷᛶ࡟ᙳ㡪ࢆ୚࠼࡚࠸ࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋࡑࡇ࡛ࠊ㸰⣔⤫࠿ࡽᚓࡽࢀࡓ ) ࣐࢘ࢫ  ༉ࢆ⏝࠸࡚ࠊ.5DV 㑇ఏᏊ QG࢖ࣥࢺࣟࣥ୰ࡢ polymorphism ࣐࣮࣮࢝ࢆ⏝࠸࡚ QTL ゎᯒࢆ⾜ࡗࡓࡀ᭷ព࡞┦㛵ࡣᚓࡽࢀ࡞࠿ࡗࡓ㸦P = 0.60㸧ࠋࡇࡢ⤖ᯝࡣࠊࡇࡢࣔࢹࣝ࡟࠾ࡅࡿ㸰⣔⤫㛫ࡢ␗࡞ࡿⓎ⒴ឤཷᛶࡢཎᅉࡀ .5DV 㑇ఏᏊⓎ⌧㔞ࡢᕪ࡛ࡣㄝ࡛᫂ࡁࡎࠊ௚ࡢᅉᏊࡀᏑᅾࡍࡿࡇ࡜ࢆ♧၀ࡋ࡚࠸ࡿࠋ⌧ᅾࠊ.5DV ࢩࢢࢼࣝ⤒㊰࡟㛵㐃ࡋࡓ㑇ఏᏊࡀࢥ࣮ࢻࡉࢀ࡚࠸ࡿᰁⰍయ㡿ᇦࢆ୰ᚰ࡟ࡉࡽ࡞ࡿゎᯒࢆࡍࡍࡵ࡚࠸ࡿࠋ12

ෆど㙾ࣉ࣮ࣟࣈࢆ⏝࠸ࡓ࣐࢘ࢫ࡬ࡢẼ⟶ࢳ࣮ࣗࣈᤄ⟶࡜྾ධ㯞㓉‫ۑ‬௒㔝වḟ㑻ࠊ␊ᒣ⨾㤶ࠊᑠᕝဴᖹࠊሷ㇂ᜤᏊ
㸦ி㒔⏘ᴗ኱Ꮫ࣭⥲ྜ⏕࿨⛉Ꮫ㒊ࠊᰴᘧ఍♫$96ࠊኟ┠〇సᡤࠊᅜ❧ᚠ⎔ჾ⑓ࢭࣥࢱ࣮◊✲ᡤ㸧࠙⫼ᬒ࡜┠ⓗࠚື≀ᐇ㦂ࡣࣄࢺ࡬ࡢእᤄࢆ୺┠ⓗ࡜ࡋ࡚⾜ࢃࢀࡿࡀ㸪࣐࢘ࢫ࡬ࡢእ⛉ⓗฎ⨨ࢆ┠ⓗ࡜ࡋࡓ㯞㓉ࡣࣄࢺ࡜␗࡞ࡾ㸪㯞㓉῝ᗘࡢㄪ⠇ࡀᅔ㞴࡞ὀᑕ㯞㓉ࡀ୍⯡ⓗ࡛࠶ࡿ㸬ࡑࡢ୍ᅉࡣయࢧ
࢖ࢬࡀᑠࡉ࠸஦࡛࠶ࡿ㸬≉࡟Ẽ⟶ࢳ࣮ࣗࣈࡢᤄ⟶ࢆせࡍࡿ྾ධ㯞㓉ࡢᑟධࡀ኱ࡁࡃ㐜ࢀ࡚࠸ࡿ㸬ࡑࢀࢆᨵၿࡍ࡭ࡃ㸪㏆ᖺ㸪ෆど㙾ࣉ࣮ࣟࣈࢆ⏝࠸ࡓᐇ㦂ື≀࡬ࡢẼ⟶ࢳ࣮ࣗࣈᤄ⟶ࢩࢫࢸ࣒ࡀ㛤Ⓨࡉࢀࡓ㸬ࡑࡇ࡛㸪௒ᅇ㸪࣐࢘ࢫࡀฟ᮶ࡿࡔࡅⱞ③ࢆకࢃࡎ㸪ື≀ឡㆤࡢ⢭⚄࡟๎ࡗࡓ⌮᝿ⓗ࡞㯞㓉᪉ἲࢆ㛤Ⓨࡍࡿ஦ࢆ┠ⓗ࡜ࡋ࡚㸪᭱㏆Ⓨ኎ࡉࢀࡓ࣐࢘ࢫẼ⟶ࢳ࣮ࣗࣈᤄ⟶⏝ࡢෆど㙾ࢩࢫࢸ࣒࡜㸪ࡑࢀࢆ⏝࠸ࡓ྾ධ㯞㓉ࡢ᭷⏝ᛶࡢ᳨ウࢆ⾜ࡗࡓ㸬࠙ᮦᩱ࡜᪉ἲࠚ&%/࣐࢘ࢫࡢ࢜ࢫ  ༉㸦య㔜㸸㹼J㸪㐌㱋㸸 㐌㸧ࢆ⏝࠸࡚⾜ࡗࡓ㸬๓ᢞ୚࡜ࡋ࡚◲㓟࢔ࢺࣟࣆࣥ
PJNJ ࡜࣌ࣥࢺࣂࣝࣅࢱ࣮ࣝ PJNJ ࢆ⭡⭍ෆᢞ୚ࡋ㸪ࠕ$96⣽ᚄෆど㙾ࢩࢫࢸ࣒ 7(6$/$ࠑࢸࢧࣛࠒ$(&ࠖࢆ⏝࠸࡚ᤄ⟶ࢆヨࡳ㸪ᤄ⟶ᚋࡣࡆࡗṑ㢮⏝㯞㓉ࢩࢫࢸ࣒ 5&㸦9(7(48,3 ♫〇㸧࡜࣐࢘ࢫ࣭ࣛࢵࢺ➼ᑠື≀ᐇ㦂⏝ேᕤ࿧྾ჾ6/$9HQWLODWRU㸦ኟ┠〇సᡤ㸧࡟᥋⥆ࡋ࡚࢖ࢯࣇࣝࣛࣥࢆ ࡛྾ධ㯞㓉ࢆ⥔ᣢࡋࡓ㸬࡞࠾㸪⾡୰ࡢ࣐࢘ࢫࣔࢽࢱࣜࣥࢢࡣ 0RXVH2;3OXV㸦67$55 ♫〇㸧ࢆ⏝࠸࡚࣐࢘ࢫࡢ 9LWDO6LJQ ࢆ☜ㄆ࣭グ㘓ࡋࡓ㸬࠙⤖ᯝ࡜⪃ᐹࠚ௒ᅇࡢᐇ㦂࡛ࡣ඲࡚ࡢಶయ࡛  ศ௨ෆ࡟ᤄ⟶ࡀᡂຌࡋࡓ㸬ࡲࡓ㸪㯞㓉ᅇ㊰࡬᥋⥆ᚋࡣື⬦ෆ㓟⣲㣬࿴ᗘ㸦6S2㸧ࡀ௨ୖࢆ♧ࡋ㸪ࡑࡢ௚ࡢ 9LWDO6LJQ ࡶ㠀ᖖ࡟Ᏻᐃࡋ࡚࠸ࡓ㸬௨ୖࡢ⤖ᯝ࠿ࡽ㸪ᮏࢩࢫࢸ࣒ࢆ⏝࠸ࡿ஦࡟ࡼࡾᏳᐃࡋࡓ㯞㓉≧ែࡀᚓࡽࢀࡿ஦ࡀ♧၀ࡉࢀࡓ㸬

13ṇᖖ࣮࢝ࣟࣜࡢ㧗⬡⫫࣭㧗⢾㉁㣗ࡀ :++/0, ࢘ࢧࢠ࡟ཬࡰࡍᙳ㡪‫ ࣥ࢖࢘ࣘۑ‬㸪ᑀ༤ 㸪⋤ᑠ⇩ 㸪$KPHG%LODO:DTDU㸪ᑠụᬛஓ 㸪ሷぢ㞞ᚿ 㸪⠊Ụᯘ 㸦 ⚄ᡞ኱㝔࣭་࣭ື≀ᐇ㦂᪋タ㸪 ᒣ᲍኱㝔࣭་࣭ศᏊ⑓⌮Ꮫ㸪⚄ᡞ኱㝔࣭་࣭⑌ᝈࣔࢹࣝື≀⑓ែ⏕⌮Ꮫ㸧࠙⫼ᬒ࡜┠ⓗࠚ⬡㉁ࡸ⢾㉁ࡢ㐣๫ᦤྲྀࡣࠊ⫧‶ࡸ࣓ࢱ࣎ࣜࢵࢡࢩࣥࢻ࣮࣒ࣟࡢせᅉ࡜࡞
ࡿࠋపẚ㔜࣏ࣜ⺮ⓑ /'/ ཷᐜయࡢ㑇ఏⓗ␗ᖖ࡟ࡼࡾⓎ⑕ࡍࡿᐙ᪘ᛶ㧗ࢥࣞࢫࢸ࣮ࣟࣝ⾑⑕ )+ ࡢᝈ⪅ࡣࠊ⎔ቃせᅉ㸦㣗⩦័➼㸧࡟ᑐࡍࡿᙳ㡪ࡶཷࡅࡸࡍ࠸ࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿࠋࡋ࠿ࡋࠊ㣗஦㔞ࢆไ㝈ࡋ࣮࢝ࣟࣜࡢ㐣๫ᦤྲྀ࡟࡞ࡽࡠࡼ࠺⬡㉁ࡸ⢾㉁ࢆᦤྲྀࡋࡓሙྜࡢᙳ㡪ࡣ୙࡛᫂࠶ࡿࠋᮏ◊✲࡛ࡣࠊ)+ ࡢື≀ࣔࢹ࡛ࣝ࠶ࡿ :++/0, ࢘ࢧࢠࢆ⏝࠸࡚ࠊ㧗⬡⫫࣭㧗⢾㉁㣗ࡢไ㝈ᦤྲྀࡀཬࡰࡍᙳ㡪࡟ࡘ࠸᳨࡚ウࡋࡓࠋ࠙᪉ἲࠚ ࣨ᭶㱋ࡢ࢜ࢫࡢ :++/0, ࢘ࢧࢠ࡟ࠊࣖࢩἜ࡜ ࡢࣇࣝࢡࢺ࣮ࢫࢆ
ῧຍࡋࡓ㣵ࢆ  㐌㛫ᢞ୚ࡋࠊ⾑୰⬡㉁ࠊ࢖ࣥࢫࣜࣥឤཷᛶࠊෆ⮚⬡⫫ࡢኚ໬ࢆࠊྠ㔞ࡢ࣮࡛࢝ࣟࣜ㏻ᖖ㣗ࢆᦤྲྀࡋࡓ :++/0, ࢘ࢧࢠ࡜ẚ㍑ࡋࡓࠋ࠙⤖ᯝࠚ୧⩌࡛ྠ㔞ࡢ࣮࢝ࣟࣜᦤྲྀ࡜࡞ࡿࡼ࠺⤥㣵㔞ࢆㄪᩚࡋࡓ⤖ᯝࠊᐇ㦂ᮇ㛫ࡢయ㔜ࡢ᥎⛣ࡣྠ⛬ᗘ࡛࠶ࡗࡓࠋࡋ࠿ࡋࠊ㧗⬡⫫࣭㧗⢾㉁㣗ࢆᦤྲྀࡋࡓ
:++/0, ࢘ࢧࢠ࡟࠾࠸࡚ࠊ㧗⬡⾑⑕ࡢቑᝏࠊ࢖ࣥࢫࣜࣥឤཷᛶࡢῶᙅࠊෆ⮚⬡⫫㔞ࡢቑຍࠊ⬡⫫⫢ࡢⓎ⏕ࡀㄆࡵࡽࢀࡓࠋ࠙⪃ᐹࠚ௨ୖࡢ⤖ᯝࡼࡾࠊ࣮࢝ࣟࣜࡢ㐣๫ᦤྲྀ࡟࡞ࡽࡠࡼ࠺㧗⬡⫫࣭㧗⢾㉁㣗ࢆᦤྲྀࡋࡓሙྜ࡛ࡶࠊ)+ ࣔࢹࣝࡢ :++/0, ࢘ࢧࢠ࡟࠾࠸࡚ࠊ࣓ࢱ࣎ࣜࢵࢡࢩ
ࣥࢻ࣮࣒ࣟᵝࡢ⑓ែࢆⓎ⑕ࡍࡿࡇ࡜ࡀ᫂ࡽ࠿࡟࡞ࡗࡓࠋࡑࡢࡓࡵࠊ⎔ቃᅉᏊ࡬ࡢឤཷᛶࡀ㧗࠸࡜࠸࠺㑇ఏⓗ⣲ᅉࢆ᭷ࡍࡿሙྜࠊ㣗஦ࡢ㔞ࡔࡅ࡛࡞ࡃ㉁࡟ࡶὀពࢆᡶࢃ࡞ࡅࢀࡤ࡞ࡽ࡞࠸ࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ

14ᛴᛶෙ⑕ೃ⩌ㄏⓎࣔࢹࣝࠊWHHLMI ࢘ࢧࢠࠐᑠụᬛஓ 㸪ఀ⸨㝯 㸪ᒣ⏣ᝅኈ ࠊᅜྜྷಙᜨ ࠊᑠᯘດ 㸪ሷぢ㞞ᚿ 12

( ⚄ᡞ኱㝔࣭་࣭ື≀ᐇ㦂᪋タ㸪 ⚄ᡞ኱㝔࣭་࣭⑌ᝈࣔࢹࣝື≀⑓ែ⏕⌮Ꮫ)䛆⫼ᬒ䛸┠ⓗ䛇ᚰ⾑⟶⑌ᝈ䛿ୡ⏺䛾Ṛᅉ䛾➨୍఩䛷䛒䜚䚸୰䛷䜒ᛴᛶᚰ➽᱾ሰ䚸୙Ᏻᐃ⊃ᚰ⑕䚸ᚰ✺↛Ṛ䛾⥲⛠䛷䛒䜛ᛴᛶෙ⑌ᝈ䠄acute coronary syndromes, ACS䠅䛜㔜
せ䛸䛥䜜䛶䛔䜛䚹䛧䛛䛧䚸䝠䝖 ACS 䛻ᑐᛂ䛧䛯䝰䝕䝹ື≀䛿㛤Ⓨ䛥䜜䛶䛔䛺䛔䛣䛸䛛䜙䚸䛭䛾Ⓨ⑕ᶵᗎ䛾ヲ⣽䛻䛴䛔䛶䛿୙᫂䛷䛒䜛䚹ᮏ◊✲䛷䛿䚸ື⬦◳໬䛾㐍ᒎ䛻䜘䜛ෙື⬦㛢ሰ䛜ཎᅉ䛷ᚰ➽᱾ሰ䜢Ⓨ⑕䛩䜛 WHHLMI 䜴䝃䜼䜢⏝䛔䛶
WHHLMI 䜴䝃䜼䛜 ACS 䛾䝰䝕䝹ື≀䛻䛺䜛䛛䛹䛖䛛䜢ㄪ䜉䛯䚹䛆᪉ἲ䛇㯞㓉ୗ䛾 WHHLMI 䜴䝃䜼䜢⏝䛔䚸䝜䝹䜶䝢䝛䝣䝸䞁䜢ᣢ⥆㟼ὀୗ䛻䜶䝹䝂䝜䝡䞁䜢㟼ὀ䛧䚸ෙ䝇䝟䝈䝮䛾ㄏⓎ䜢ヨ䜏䛯䚹ෙ䝇䝟䝈䝮䛾Ⓨ⏕䛿ᚰ㟁ᅗ䛸ෙື⬦㐀ᙳ䛷☜ㄆ䛧䛯䚹ᚰᶵ⬟䛿ᚰ䜶䝁䞊⿦⨨䜢⏝䛔䛶ᕥᐊ䛾㐠ືᗘ䜢ホ౯䛧䚸ᚰ➽䛾⹫⾑യᐖ䛿⾑Ύ୰䛾 heart-type FABP䚸䝖䝻䝫䝙䞁䠉I䚸䝭䜸䜾䝻䝡䞁⃰ᗘ䛷ホ౯䛧䛯䚹ෙ䝇䝟䝈䝮ㄏⓎᚋ䚸㯞㓉⸆䛾㐣㔞ᢞ୚䛷䜴䝃䜼䜢ᏳᴦṚ䛧䚸ෙື⬦䛾⑓⌮⤌⧊ᶆᮏ䜢స〇䛧䛯䚹䛆⤖ᯝ䛇⾑⟶཰⦰๣䛾ే⏝ᢞ୚䛻䜘䜚䚸ᚰ㟁ᅗ䛷 ST పୗ䚸T Ἴ㏫㌿䚸ᚰᐊᛶ୙ᩚ⬦䛜ほᐹ䛥䜜䚸ෙື⬦㐀ᙳ䛷䝇䝟䝈䝮䛾Ⓨ⏕䜢☜ㄆ䛧䛯䚹䝇䝟䝈䝮䜢ㄏⓎ䛧䛯䜴䝃䜼䛷䛿䚸ᕥᐊ䛾㐠ືᗘ䛜పୗ䛧䚸⾑Ύᚰ➽⹫⾑䝬䞊䜹䞊䛜ୖ᪼䛧䛯䚹ෙື⬦䛾⑓⌮⤌⧊ゎᯒ䛷䛿䚸䝬䜽䝻䝣䜯䞊䝆ṧ´䛻ᐩ䜐⑓ኚ䛾◚⿣䛸䛭䜜䛻⥆䛟㛢ሰᛶ䛾⾑ᰦ䜢ㄆ䜑䛯䚹䜎䛯䚸ෆ⓶⣽⬊䛾ᾘኻ䛸ᾘኻ㒊఩䛛䜙䛾䝬䜽䝻䝣䜯䞊䝆䛾ὶฟ䛜ほᐹ䛥䜜䛯䚹䝇䝟䝈䝮䜢ㄏⓎ䛷䛝䛺䛛䛳䛯䜴䝃䜼䛷䛿䛣䜜䜙䛾ኚ໬䛿ㄆ䜑䜙䜜䛺䛛䛳䛯䚹䛆⤖ㄽ䛇
WHHLMI 䜴䝃䜼䛻䛚䛔䛶䛿䚸ෙ䝇䝟䝈䝮䛾ㄏⓎ䛻䜘䛳䛶ෙື⬦䝥䝷䞊䜽䛜◚⥢䛧䚸ACS 䛾䝰䝕䝹ື≀䛻䛺䜛ྍ⬟ᛶ䛜♧၀䛥䜜䛯䚹ࠕࠞࡂ࡜ࠗࡕ࡝ߩࡕ࠺࡞േ‛ൻߦะߌߚ⾗Ḯ࡮ᛛⴚ࡮ᖱႎၮ⋚ߩ⎇ⓥ㧦ᄖ்ᕈ∔ᖚߩᴦ≮ߣౣ↢⎇ⓥ߳ߩㆡ↪ࠍⷞ㊁ߦජ⪲ ⷫᢥ 㧔╳ᵄᄢቇ ↢๮ⅣႺ♽࡮⣖␹⚻ᖱႎቇಽ㊁࡮ౣ↢↢ℂቇ⎇ⓥቶ㧕ࠗࡕ࡝ߪਔ↢✁࡮᦭የ⋡ߦዻߔ⣄ᬁേ‛ߢ޽ࠆ‫ߩ࡚ࠝ࠙࠙ࠪࡦࠨ߿࡞ࠛࠞޔࠅ߹ߟޕ‬ખ㑆ߢ޽ࠆ‫ޔߒ߆ߒޕ‬ㄭ✼ߩࠨࡦ࡚ࠪ࠙࠙ࠝ㘃ߣߪ⚂1 ం 6000 ਁᐕ೨(ࠫࡘ࡜♿ᓟᦼ)ߦ೎ࠇߚߣ⠨߃ࠄࠇߡ߅ࠅ‫ߚߒ┙⁛ޔ‬ಽ㘃⟲‫⑼࡝ࡕࠗޟ‬㧔Salamandridae㧕‫ࠍޠ‬᭴ᚑߒߡ޿ࠆ‫࡝ࡕࠗޕ‬⑼ߩਛߢ߽‫ࠅࠃޔ‬ㅴൻߒߚ৻⟲߇‫ࠗࠆࠁࠊ޿ޔ‬ࡕ࡝㧔Newt㧕ߣ๭߫ࠇࠆ㧔D. W. Weisrock et al.,
Molecular Phylogenetics and Evolution 41:

ࠕࠞࡂ࡜ࠗࡕ࡝ Cynops pyrrhogaster368-383, 2006㧕‫ޕ‬ࠗࡕ࡝ߪታ㛎↢‛ቇ߇ᆎ߹ߞߚ 18 ਎♿߆ࠄ⎇ⓥߐࠇߡ޿ࠆ‫ߩࠕ࡝࠲ࠗޕ‬ඳ‛ቇ⠪
Lazaro Spallanzani㧔1729-1799㧕ߦࠃࠆࠗࡕ࡝࡮ࠨࡦ࡚ࠪ࠙࠙ࠝߩౣ↢ታ㛎ߪ᦭ฬߢ‫ޔ‬ᓐ߇⷗಴ߒߚ‫ޟ‬േ‛ߩౣ↢⢻ജ߇㦂ߣߣ߽ߦૐਅߔࠆ‫ߩ࠾࡯ࠔ࠷ࡦ࡜ࡄࠬޟߪ⽎⃻ޠ‬ᴺೣ‫ޠ‬ߣߒߡ⍮ࠄࠇߡ޿ࠆ‫ޕ‬ࠗࡕ࡝ߩ⚦⢩ߪઁߩ⣄ᬁേ‛ࡕ࠺࡞ߣ⇣ߥࠅ‫ޔ‬ᄢ߈ߥ⚦⢩૕‫ޔ‬ᄢ߈ߊߡ᣿⍎ߥᩭߣᨴ⦡
૕㧔24 ᧄ㧕‫ߚߒߣࠅߊߞࠁޔ‬ಽⵚ๟ᦼ‫ޔ‬ઍ⻢߇ૐߊߡ࠲ࡈ‫․ߩߤߥޔ‬ᓽ߆ࠄ‫ޔ‬᭽‫↢ߥޘ‬๮⃻⽎ߦኻߔࠆಽሶ࡮⚦⢩࡮↢ℂ࡟ࡌ࡞ߩ⎇ⓥߦ߅޿ߡᤨ㑆࡮ⓨ㑆ಽ⸃⢻ߩ㜞޿⸃ᨆߦ᦭೑
ߢ޽ࠆ‫ޕ‬⢦߿ᚑ૕⚵❱ߩ୘૕㑆⒖ᬀ߇ኈᤃߥὐ߽ߎߩേ‛ࠍ↪޿ࠆ೑ὐߢ޽ࠅ‫ޔ‬ታ㓙‫ޔ‬Hans
Spemann ߩ⢦ᠲ૞ߦࠃࠆࠝ࡯ࠟ࠽ࠗࠩ࡯ߩ⊒⷗㧔ࡁ࡯ࡌ࡞කቇ↢ℂቇ⾨‫ޔ‬1935 ᐕ㧕ߦነਈߒߚߣ⸒߃ࠆ‫ޔߚ߹ޕ‬వㅀߩࠃ߁ߦ‫ߩߎޔ‬േ‛ߩථ⿧ߒߚౣ↢⢻ജߪ᦭ฬߢ‫ޔ‬૕ߩਥⷐߥㇱಽ㧔body-parts㧕߇ౝ࿷ߔࠆ⚵❱ᐙ⚦⢩ߛߌߢߥߊ‫⚳ޔ‬ಽൻ૕⚦⢩ߩ⣕ಽൻ࡮ಽൻォ឵㧔࠽࠴ࡘ࡜࡞ߥ࡝ࡊࡠࠣ࡜ࡒࡦࠣ㧕ࠍ੺ߒߡቢోౣ↢ߔࠆߚ߼‫ߪ࡝ࡕࠗޔ‬⣄ᬁേ‛ߦ߅ߌࠆౣ↢ߩࡕ࠺࡞‫‘ߪ޿ࠆ޽ޔ‬Master of Regeneration’ߣ๭߫ࠇߡ޿ࠆ㧔Casco-Robles et al.,Nature Protocols 6: 600-608, 2011㧕‫ߩߎޔߦࠄߐޕ‬േ‛ߪ᳓߆ࠄ㒽ߦㅴ಴ߒߚೋᦼߩ⣄ᬁേ‛㧔྾⿷േ‛ߩ␲వ㧕ߩ૕೙ࠍ஻߃ߡ߅ࠅ‫ޔ‬⣄ᬁേ‛ߩ㒽਄ㆡᔕ࡮ㅴൻߩᱧผࠍ⍮ࠆ਄ߢ߽㊀ⷐߥ૏⟎ߦ޽ࠆ‫ߥ߁ࠃߩߎޕ‬೑ὐ࡮․ᓽ߆ࠄ‫ߪ࡝ࡕࠗޔ‬ᢙ‫ߩޘ‬ᱧผ⊛⊒⷗ߦ⽸₂ߒ‫⃻ޔ‬࿷ߢ߽↢๮ᵴേߩ᥉ㆉේℂ߿ᣂ᭎ᔨߩតⓥߦ߅޿ߡ breakthrough ߦߟߥ߇ࠆᢙᄙ
ߊߩ⊒⷗߇ᦼᓙߐࠇࠆേ‛ߢ޽ࠆ‫ޕ‬㧝㧚ᚒ߇࿖ߩࠗࡕ࡝⎇ⓥᚒ߇࿖ߩࠗࡕ࡝⎇ⓥߪ‫߅‘ޔ‬ኅ⧓’ߣ⹏ߐࠇࠆ߶ߤᱧผߣ⫾Ⓧ߇޽ࠅ‫ޔ‬ᢙᄙߊߩ⪺ฬߥ⎇ⓥ⠪ࠍヘ಴ߒߡ߈ߚ‫ޔ߫߃଀ޕ‬ౣ↢⎇ⓥߩᳯญ๋ᦶߣጟ↰▵ੱ‫ޔ‬ฃ♖⎇ⓥߩ㝩ᱞ৻ᄦ‫࡮↢⊒ޔ‬ಽൻ⎇ⓥߩᵻፉ⺈㧔ಽൻ⺃ዉ࿃ሶ‫⷗⊒ߩޠࡦࡆ࠴ࠢࠕޟ‬㧕‫↢ޔ‬ℂ࡮ⴕേ⎇ⓥߩ⩵ጊ᭢㧔⣄ᬁേ‛ߢೋ߼ߡߩࡍࡊ࠴࠼ࡈࠚࡠࡕࡦ‫⷗⊒ߩޠࡦ࡝ࡈ࠺࠰ޟ‬㧕╬ߢ޽ࠆ‫⃻ޕ‬࿷ߢ߽‫↢ޔ‬๮⑼ቇߩᐢ▸ߥ㗔ၞߢߘߩ⾰࡮㊂ߣ߽਎⇇ࠍ࿶ୟߒߡ޿ࠆ‫ޔ߫߃଀ޕ‬቟ㇱ⋿৻࡮ᳯ㗡ᕡ㧔ᾢᧄᄢ㧕ߩ♖ሶᒻᚑᯏ᭴ߩ⎇ⓥ‫ޔ‬ᷰㆺ᣿ᒾ㧔ጊᒻᄢ㧕ߩ૕ౝฃ♖᭽ᑼߣ♖ሶㆇേ⺞▵ᯏ᭴ߩ⎇ⓥ‫↰ޔ‬ਛṑᐽ㧔㕒ጟᄢ㧕ߩ↢ᱺౝಽᴲߣ᳓ઍ⻢ߩ⎇ⓥ‫ޔ‬ጤየᐽብ࡮਄㊁⑲৻㧔ጊญᄢ㧕ߩෆ⊒↢㐿ᆎᯏ᭴ߣೋᦼ⢦⚦⢩๟ᦼߩ⎇ⓥ‫ޔ‬ᵻፉ⺈࡮㆏਄㆐↵㧔᧲ᄢ㧕࡮᦭ᴰ㜞ผ㧔₹Ꮉᄢ㧕ߩེቭᒻᚑᯏ᭴ߣ⤳ེഃ⵾⎇ⓥ‫ޔ‬ዊ⇌⑲৻㧔ർ㉿ᄢ㧕ߩේ⣺ᒻᚑߣ⚦⢩ㆇേᯏ᭴ߩ⎇ⓥ‫┻ޔ‬ፉ৻ੳ㧔ฬᄢ㧕ߩེቭᒻᚑ⺃ዉࠪࠣ࠽࡞ࡀ࠶࠻ࡢ࡯ࠢߩ⎇ⓥ‫⦱ޔ‬Ꮉᶈ㧔⑔ጟ⋵┙ᄢ㧕࡮㒙ᒻᷡ๺㧔੩ᄢ㧕࡮ㄭ⮮ᄈੱ㧔㒋ᄢ㧕࡮’ା቟㧔㒋ᄢ࡮ߐ߈߇ߌ㧕ߩ྾⢇‫ޔ‬⣖߅ࠃ߮᳓᥏
૕ౣ↢ߩ⎇ⓥ‫ޔ‬ජ⪲ⷫᢥ㧔╳ᵄᄢ㧕࡮⨹ᧁᱜ੺㧔ᄹ⦟ᅚሶᄢ㧕࡮ਭ౷ୃ㧔㒋ᄢ㧕ߩ✂⤑ౣ↢ߩ⎇ⓥ‫ޔ‬ᨋ೑ᙗ㧔㠽ขᄢ㧕ߩᔃ⤳ౣ↢ߩ⎇ⓥ‫ࠃߺ߰↰⼾ޔ‬㧔ᄹ⦟⋵┙කᄢ㧕࡮╴੗๺⟵㧔ᣧⒷ↰ᄢ㧕ߩᣂⷙ⣖ࡎ࡞ࡕࡦߩหቯߣⴕേ⊒⃻࡮⺞▵ᯏ᭴ߩ⎇ⓥ‫ޔ‬ජ⪲◊ᒾ㧔਄ᥓᄢ㧕ߩᣣ๟ᦼᵴേߩಽሶᯏ᭴ߩ⎇ⓥ‫ୖޔ‬ᯅ㓉㧔㒋ᄢ㧕࡮ਛ⼱ᢘ㧔╳ᵄᄢ㧕ߩᗵⷡฃኈᯏ᭴ߩ⎇ⓥ‫ޔ‬ጊਅ߹ࠅ㧔᧲ർᄢ㧕ߩ࠹࠻ࡠ࠼࠻ࠠࠪࡦวᚑ⚻〝ߩ⎇ⓥ‫ޕࠆ޽ߢޘ╬ޔ‬ࠗࡕ࡝ߩ㜞޿᦭↪ᕈߪߎ߁ߒߚ⎇ⓥታ❣ߦࠃࠅ⸽᣿ߐࠇߡ޿ࠆ‫ޕ‬ߒ߆ߒ‫ޔ‬
ᱷᔨߥߎߣߦ‫ޔ‬ㄭᐕ‫ࠍ࡝ࡕࠗޔ‬ᛒ߁⎇ⓥ⠪㧔․ߦ⧯ᚻ⎇ⓥ⠪㧕ߪᷫዋߒߡ޿ࠆ‫ߩߘޕ‬ਥߥේ࿃ߪ‫ࡕߩઁޔ‬࠺࡞േ‛ߦᲧߴ೑↪ߢ߈ࠆㆮવሶᖱႎ߿࠷࡯࡞߇ዋߥ޿ߎߣ‫߮ࠃ߅ޔ‬ㆮવሶᡷᄌേ‛ߩ೑↪߇࿎㔍ߥߎߣߢ޽ࠆ‫ޕ‬ᚒ߇࿖ߩࠗࡕ࡝⎇ⓥߦߪࠕࠞࡂ࡜ࠗࡕ࡝㧔Cynops pyrrhogaster㧕߇૶↪ߐࠇߡ޿ࠆ㧔೨㗁ߩ౮⌀㧕‫࡝ࡕࠗ࡜ࡂࠞࠕޕ‬㧔⿒⣻੗቞㧕ߪᣣᧄ࿕᦭⒳ߢ޽ࠅ‫ޔ‬ฎߊ߆ࠄㄘ⠹ߩࠪࡦࡏ࡞
ߣߒߡᣣᧄੱߦⷫߒ߹ࠇߡ߈ߚ㧔‫ޟ‬㌃㐇ߩ⛗ࠍ⺒ߺ⸃ߊ‫ޔޠ‬1997 ᐕ‫ޔ‬࿖┙᳃ᣖඳ‛㙚✬‫ޔ‬ዊቇ㙚㧕‫↢ޔߒ߆ߒޕ‬ᕷᢙ߇ᷫዋߒ‫ޔ‬2006 ᐕߦߪḰ⛘Ṍෂᗋ⒳ߣߒߡ⸥タߐࠇߚ㧔ⅣႺ⋭࡟࠶࠼࡝ࠬ࠻㧕‫↢ޕ‬ᘒ࡮ⅣႺ߿♽⛔࡮ㅴൻߩⷞὐ߆ࠄਔ↢㘃ߩ଻⼔ߦขࠅ⚵߻⎇ⓥ⠪ߪዋߥߊ‫᧻ޔ‬੗ᱜᢥ࡮⷏Ꮉቢㅜ㧔੩ᄢ㧕࡮ን᳗◊㧔℄⃿ᄢ㧕߇࿾㆏ߥᵴേࠍ⛯ߌߡ޿ࠆ‫ޕ‬ߎߩࠃ߁ߦࠕࠞࡂ࡜ࠗࡕ࡝ߪ᭽‫⎇ߥޘ‬ⓥ㗔ၞߢߘߩ᦭↪ᕈ߇᣿ࠄ߆ߢ޽ࠆߦ߽㑐ࠊࠄߕ‫ޔ‬↢ᕷᢙ㧔⎇ⓥ⾗Ḯ㧕߿⎇ⓥ⠪ᢙ߇ᷫዋߒߡ޿ࠆ‫ޔߪߢ߹߹ߩߎޕ‬ᣧ᥅‫ޔ‬ᚒ߇࿖ߩ߅ኅ⧓ߢ
޽ࠆࠗࡕ࡝⎇ⓥߪㅜ⛘߃‫↢ޔ‬๮⑼ቇߩㅴዷߦ߽ᄢ߈ߊᓇ㗀ߔࠆߦ㆑޿ߥ޿‫⎇࡝ࡕࠗޕ‬ⓥߩዷ㐿ࠍ࿑ࠅ‫ߪߡ޿߭ޔ‬ᚒ߇࿖ߩ↢๮⑼ቇ᳓Ḱߩะ਄࡮ᒝൻߦߟߥߍࠆߚ߼ߦ߽‫ޔ‬ㆮવሶ߆ࠄ↢ᘒ࡮ⅣႺߦ޿ߚࠆ᏷ᐢ޿㗔ၞߩ⎇ⓥ⠪߇‫ߩࠇߙࠇߘޔ‬ಽ㊁ߩᨒࠍ⿥߃ߡ᦭ᯏ⊛ߦㅪ៤ߒ‫✚ޔ‬ജࠍ᜼ߍߡࠕࠞࡂ࡜ࠗࡕ࡝ߩ⾗Ḯൻߣࡕ࠺࡞േ‛ൻߦขࠅ⚵߻ᔅⷐ߇޽ࠆ‫ߚ߹ޕ‬หᤨߦ‫౒ࠍࠄࠇߎޔ‬ㅢၮ⋚ߣߒߡ‫ޔ‬ฦಽ㊁ߦ߅ߌࠆᣂߚߥ⎇ⓥߩഃ಴ࠍⓍᭂ⊛ߦផㅴߔࠆᔅ
ⷐ߇޽ࠆ‫⑳ޔ߼ߚߩߘޕ‬㆐ߪ↢‛ቇ࡮කቇࠍ฽߻ᐢ▸ߥ㗔ၞߩ⎇ⓥ⠪㧔40 ฬ‫ޔ‬20 ᄢቇ㧕࡮ᡰេ⠪㧔NPO ߿⥄ᴦ૕ࠍ฽߻㧕ࠍ⚿㓸ߒ‫ޔ‬㧔ᣂ㧕ࠗࡕ࡝ࡀ࠶࠻ࡢ࡯ࠢ㧔JNRC㧧ᣣᧄേ‛ቇળߩࠗࡕ࡝ࡀ࠶࠻ࡢ࡯ࠢࠍ⛮ᛚߔࠆᣂ⚵❱㧕ࠍ⸳┙ߒߚ㧔2008 ᐕ 10 ᦬㨪㧧ࡎ࡯ࡓࡍ࡯ࠫ http://imori-net.org㧕‫⃻ޕ‬࿷‫ޔ‬JNRC ߪߎߩ⋡ᮡߦะߌߡᵴേߒߡ޿ࠆ‫ޕ‬㧞㧚ࠕࠞࡂ࡜ࠗࡕ࡝ߩࡕ࠺࡞േ‛ൻߩขࠅ⚵ߺ

⑳㆐ JNRC ߪ‫⎇࡝ࡕࠗޔ‬ⓥߩᦨ㊀ⷐ⺖㗴ߩ৻ߟߢ޽ࠆࠕࠞࡂ࡜ࠗࡕ࡝ߩࡕ࠺࡞േ‛ൻࠍ⋡ᜰߒ‫⾗ޔ‬Ḯ࡮ᛛⴚ࡮ᖱႎၮ⋚ߩ⎇ⓥࠍផㅴߒߡ޿ࠆ‫ޕ‬᭽‫ߥޘ‬ᵴേࠍߒߡ޿ࠆ߇‫ߢߎߎޔ‬ߪߘߩᚑᨐࠍ㧞ߟߏ⚫੺ߔࠆ‫ޕ‬వㅀߩㅢࠅ‫ߩߘߪ࡝ࡕࠗ࡜ࡂࠞࠕޔ‬ᢙࠍᷫࠄߒߡ޿ࠆ‫ޔߚ߹ޕ‬࿾ၞߏߣߩㆮવ⊛ᄙ᭽ᕈ߇ᄢ߈޿ߎߣ߽ಽ߆ߞߡ޿ࠆ㧔Tominaga et al., Molecular Phylogenetics and Evolution
66: 654-667, 2013㧕‫ޔ߼ߚߩߘޕ‬㊁↢ࠗࡕ࡝㧔↢‛࡮ㆮવ⾗Ḯ㧕ߩ଻⼔࡮଻ో‫⎇߮ࠃ߅ޔ‬ⓥ࡮ᢎ⢒↪ࠗࡕ࡝ߩᮡḰൻߣଏ⛎૕೙ߩᢛ஻߇ᕆോߢ޽ࠆ‫ߢߎߘޕ‬ᚒ‫ޔߪޘ‬዁᧪ߦᷰࠅ‫ޔ‬ࠦࡒࡘ࠾࠹ࠖ࡯ో૕ߦචಽߥᢙߩࠗࡕ࡝㧔⑳ߩ࡜ࡏߛߌߢ߽ᐕ㑆 1000 ඘એ਄߇ᔅⷐ㧕߇ឭଏߢ߈ࠆࠃ߁ߦ‫⨙ޔ‬ၔ⋵ขᚻᏒߦደᄖ⥄ὼ❥ᱺᣉ⸳‫ߩࠅ߽޿ޟ‬㉿‫ࠍޠ‬࿾ၞ૑᳃࡮⥄ᴦ૕ߣද௛ߢ⸳⟎ߒߚ㧔2009 ᐕ 10 ᦬ 28 ᣣ㧕‫ౕޕ‬૕⊛ߦߪ‫ޔ‬30 ᐕએ਄⠹૞᡼᫈ߐࠇߡ߈ߚ㉿ጊߦ࿐߹ࠇߚḨ࿾㧔⼱ᵤ↰㧧⚂ 3 ࡋࠢ࠲࡯࡞㧕ࠍ೑↪ߒ‫↢߇࡝ࡕࠗޔ‬ᕷߒߡ޿ߚᤘ๺ 40ᐕઍߩ᳓↰ࠍౣ⃻ߒ‫ޔ‬Ⓑ૞ࠨࠗࠢ࡞ࠍ࿁ߔߎߣߢ‫❥ߩ࡝ࡕࠗޔ‬ᱺߦᦨㆡߥⅣႺߠߊࠅࠍᆎ
߼ߚߩߛ‫ޕ‬2010 ᐕ 11 ᦬ߦߪ‫ޔ‬ජ⪲⋵㡞ᎹᏒ♽⛔㧔ධ㑐᧲♽⛔㧕ࠕࠞࡂ࡜ࠗࡕ࡝ߩᚑ૕ 975඘ࠍ᳓↰ߦዉ౉ߔࠆߎߣ߇ߢ߈ߚ‫ޕ‬᧲ർ࿾ᣇᄥᐔᵗᴒ࿾㔡㧔2011ᐕ 3 ᦬㧕એ㒠‫ޔ‬ḝ᳓㊂ߩૐਅߣ᳓
⾰ߩᖡൻ߇໧㗴ߣߥߞߚ߇‫ޔ‬੗ᚭࠍ⸳⟎ߔࠆ╬ߩᎿᄦߢ૗ߣ߆ਸ਼ࠅ
⿧߃‫ޔ‬ዋᢙߢߪ޽ࠆ߇⌕ታߦቯ⌕ߒߡ߈ߡ޿ࠆ‫ޕ‬2016 ᐕ߆ࠄߩឭଏ
ࠍ⋡ᜰߒ‫⃻ޔ‬࿷߽ᚑ૕߿ᐜ↢࡮ᐜ૕ߩዉ౉ࠍ⛮⛯ߒߡ޿ࠆ‫ޕ‬ߎߩᵴേߪ᭽‫ోߢߍ߆߅ߩߘޕߚࠇߐ੺⚫ߢࠕࠖ࠺ࡔߥޘ‬࿖ߩ⥄ὼ଻⼔ᵴേኅߦ߽ᵈ⋡ߐࠇ‫ࠍ࡝ࡕࠗޔ‬฽߻↢‛ߣߘࠇࠄߩ↢ᕷⅣႺߩ଻⼔࡮଻ో߇ㅴࠎߢ߈ߡ޿ࠆߎߣߪ‫ޔ‬JNRC
ߩ⋡⊛ߣ߽ว⥌ߒ‫ޔ‬ᄢᄌሜߒ޿ߎߣߢ޽ࠆ‫ޕ‬㨇ߎࠇ߹ߢߩ⧰ഭߣ⃻࿷ߩᵴേߩ᭽ሶߪࡎ࡯ࡓࡍ࡯ࠫ㧔http://imori-net.org㧕ࠍߏⷩߊߛ
ߐ޿‫ޕ‬㨉

ࠕࠞࡂ࡜ࠗࡕ࡝ߩࡕ࠺࡞േ‛ൻࠍታ⃻ߔࠆ਄ߢ‫ޔ‬ㆮવሶᡷᄌ
ᛛⴚߩ⏕┙ߪᕆോߢ޽ࠆ‫ߪ࡝ࡕࠗޔߒ߆ߒޕ‬ㆮવሶᡷᄌ߇ߚ޿
߳ࠎ࿎㔍ߥേ‛ߣߐࠇߡ߈ߚ‫ޕ‬ฃ♖ෆߦ DNA ࠍᛂߜㄟ߻ᣇᴺ
ߢߩ࠻࡜ࡦࠬࠫࠚࡀࠪࠬߢߪ‫ࠢࠗࠩࡕޔ‬୘૕߽฽߼ߡ‫ޔ‬ᄖ᧪ㆮવሶࠍ⊒⃻ߔࠆ୘૕ࠍᓧࠆ⏕┙߇ 0.9㨪3.4㧑ߒ߆ߥߊ‫ޔ‬ታ〣࡟ࡌ࡞ߦߪ㆐ߒߡ޿ߥ߆ߞߚ‫ࠇߘޔߚ߹ޕ‬એ೨ߦ‫ޔ‬ෆ߿♖ሶࠍ㊁↢୘૕ߦ㗬ߞߡ޿ࠆߚ߼චಽߥᢙߣ⾰ߩฃ♖ෆࠍᓧࠆߩ߇㔍ߒߊ‫ߪߦࠄߐޔ‬ෆࠍᚑ૕߹ߢቶౝߢ⢒ߡ޽ߍࠆᣇᴺ߽⏕┙ߒߡ޿ߥ߆ߞߚ‫⑳ߢߎߘޕ‬㆐ߪ‫ߕ߹ޔ‬ቶౝߢ㐳ᦼ㘺⢒ߒߚ୘૕߆ࠄല₸ࠃߊฃ♖ෆࠍᓧࠆᣇᴺ
ߣ‫ޔ‬ᐜ↢࡮ᐜ૕ߩ㘺⢒ᴺߩᬌ⸛ࠍ㊀ߨ‫߽ߡ޿߅ߦࠇߕ޿ޔ‬᭽‫ߢࡏ࡜ߥޘ‬ታ〣น⢻ߥല₸ߩ
ࠃ޿ᣇᴺࠍ⏕┙ߔࠆߎߣ߇ߢ߈ߚ‫ޕ‬ᰴߦ‫ޔ‬᭽‫ࠢ࠶࠾ࠚࠫࠬࡦ࡜࠻ߥޘ‬ᛛⴚߩᬌ⸛ࠍⴕ޿‫ޔ‬I-SceI ࡔࠟ࠿ࠢ࡟ࠕ࡯࠯ࠍ↪޿ߚᣇᴺߩ᦭ലᕈࠍ⸽᣿ߒߚ‫ߩߎޕ‬ᣇᴺߣవߩ㘺⢒ᴺࠍวࠊߖࠆߣ‫ోޔ‬りߦᄖ᧪ㆮવሶࠍ⊒⃻ߔࠆᚑ૕୘૕ࠍ⚂ 20㧑ߩ⏕┙ߢᓧࠆߎߣ߇ߢ߈ࠆ‫ࠇߎޕ‬ࠄߩᛛⴚߪ⇼޿ߥߊ breakthrough ߢ޽ࠆߣ⸒߃ࠆ‫ޕ‬㨇ߎࠇࠄߩᛛⴚߪ‫ޔ‬Nature Protocols 6: 600-608, 2011‫⎇ޔ‬ⓥ⠪߇ᢎ߃ࠆേ‛㘺⢒ ╙ 3Ꮞ㧙࠙࠾㧘࠽ࡑࠦ߆ࠄ⣄ᬁേ‛߳㧙㧔ᣣᧄᲧセ↢ℂ↢ൻቇળ✬㧕‫┙౒ޔ‬಴ ‫ޔ‬2012‫ߦޔ‬ឝタߒ߹ߒߚ㨉

⃻࿷‫ߩࠄࠇߎޔ‬ၮ⋚⎇ⓥࠍ‫ࠆ޿↪ࠍ࡝ࡕࠗޔ‬᭽‫⎇ߥޘ‬ⓥಽ㊁ߦዷ㐿ߔࠆߚ߼ߦ‫࡝ࡕࠗޔ‬⎇ⓥࠍ‧ᒁߔࠆ 4 ಽ㊁㧔↢ᱺ‫ޔ↢⊒ޔ‬ౣ↢‫↢ޔ‬ℂ㧕ߦὶὐࠍ޽ߡ‫ࠢ࠶࠾ࠚࠫࠬࡦ࡜࠻ޔ‬ᛛⴚࠍㆡ↪ߔࠆᔕ↪ᛛⴚߩ㐿⊒ࠍㅴ߼ߡ޿ࠆ‫ ࠄࠇߎޔߚ߹ޕ‬2 ߟߦട߃‫ޔ‬JNRC ߪࠕࠞࡂ࡜
ࠗࡕ࡝ߩㆮવሶᖱႎߩ෼㓸ࠍㅴ߼ߡ޿ࠆ‫ߩߎޕ‬േ‛ߩࠥࡁࡓࠨࠗ࠭ߪ⚂ 40Gbp ߣផቯߐࠇߡ޿ࠆߚ߼‫߁ߘߪߦࡓࡁࠥޔ‬ᤃ‫ߪߣޘ‬ᚻ߇಴ߥ޿‫⑳ߢߎߘޕ‬㆐ߪ‫⎇ޔ‬ⓥ਄ࠃࠅᔅⷐᕈ߇
㜞޿㧔೑↪ଔ୯ߩ㜞޿㧕ォ౮↥‛ߩᖱႎࠍ mRNA-Seq ⸃ᨆߦࠃࠅ෼㓸ߒ‫ࠬ࡯ࡌ࠲࡯࠺ޔ‬ൻࠍㅴ߼ߡ޿ࠆ‫ޕ‬㧟㧚ᄖ்ᕈ∔ᖚߩᴦ≮ߣౣ↢⎇ⓥ߳ߩㆡ↪㧦✂⤑ࠍਛᔃߦ

⑳ߩ࡜ࡏߢߪ‫ޔ‬JNRC ߇㐿⊒ࠍㅴ߼ࠆ⾗Ḯ࡮ᛛⴚ࡮ᖱႎၮ⋚ࠍ‫ޔ‬ౣ↢⎇ⓥߦㆡ↪ߒߡ޿ࠆ‫ޕ‬ᣢㅀߩㅢࠅ‫ߪ࡝ࡕࠗޔ‬૕⚦⢩ߩ⣕ಽൻ㧔࡝ࡊࡠࠣ࡜ࡒࡦࠣ㧕ߣౣಽൻ㧛ಽൻォ឵ࠍ੺ߒߚߘߩථ⿧ߒߚౣ↢⢻ജ߆ࠄ‫ޔ‬⣄ᬁേ‛ߦ߅ߌࠆౣ↢ߩࡕ࠺࡞ߣߐࠇߡ޿ࠆ‫ޔߒ߆ߒޕ‬ߎࠇ߹ߢ⎇ⓥၮ⋚߇ਲߒ߆ߞߚߎߣ߆ࠄ‫ޔ‬ౣ↢⚵❱࡮ེቭߩ⿠Ḯߣߥࠆ⚦⢩⒳‫ޔ‬ౣ↢㐿ᆎࡔࠞ࠾࠭ࡓ‫ޔ‬⣕ಽൻࡔࠞ࠾࠭ࡓ‫ޔ‬ౣ↢⿠Ḯ⚦⢩↱᧪ߩᄙ⢻ᕈ⚦⢩ߩ․ᓽ‫ޔ‬ᒻᘒᒻᚑ㧔ࡄ࠲࡯࠾ࡦࠣ㧕ࡔࠞ࠾࠭ࡓ‫ޔ‬ౣ↢⚵❱࡮ེቭߣᧄ૕ߣߩធ⛯࡮⛔วߦࠃࠆ↢ℂᯏ⢻࿁ᓳㆊ⒟ߩ
⹦⚦ߪᧂߛᄙߊ߇⻘ߩ߹߹ߢ޽ࠆ‫ޔߜࠊߥߔޕ‬ᐙ⚦⢩⎇ⓥߣ⒖ᬀ࡮ౣ↢ක≮߇⪇‫ߊߒޘ‬ዷ㐿ߔࠆ⃻࿷ߦ߅޿ߡ߽‫ޔ‬ౣ↢ࠍ࠽࠴ࡘ࡜࡞ߦታ〣ߔࠆേ‛ߩᚢ⇛ߦߟ޿ߡߪ‫ޔ‬ታߪ߶ߣࠎ
ߤ૗߽ಽ߆ߞߡ޿ߥ޿ߣ⸒ߞߡ޿޿⃻⁁ߥߩߢ޽ࠆ‫ޕ‬⑳ߪ‫ࠍ࠻ࡅߣ࡝ࡕࠗޔ‬Ყセߒ‫ޔ‬ౣ↢‫ߩޠ޿ߥ߈ߢޟޠࠆ߈ߢޟ‬㆑޿ࠍὂࠅ಴ߔߎߣߢ‫ޔ‬ࠗࡕ࡝ߩ㜞޿ౣ↢⢻ജߩℂ⸃ߣක≮߳ߩᔕ↪ࠍ⋡ᜰߒߡ޿ࠆ‫ ߪ⑳ޔߦ߼ߚߩߘޕ‬10 ᐕએ਄ߦᷰࠅ‫ⷡⷞ࡮⤑✂ߩ࡝ࡕࠗޔ‬ౣ↢♽ߦ⌕⋡ߒߡ߈ߚ‫ߩߎޕ‬ౣ↢♽ߪ‫ޔ‬㜞ᰴᗵⷡ࡮ਛᨔ␹
⚻♽ߩౣ↢ࡕ࠺࡞ߣߥࠆ߫߆ࠅߢߥߊ‫⚛⦡⤑✂ޔ‬਄⊹㧔RPE㧕⚦⢩ߣ๭߫ࠇࠆන৻⒳ߩ⚦⢩߆ࠄౣ↢߇ᆎ߹ࠆߚ߼‫ߩ࡝ࡕࠗޔ‬᭽‫ ߥޘ‬body-parts ౣ↢ߩၮᧄේℂࠍන⚐ൻߒߡ⸃ߊࠃ
޿ታ㛎♽ߢ޽ࠆ‫ޔߚ߹ޕ‬Ⴧᱺᕈ⎣ሶ૕✂⤑∝㧔PVR㧕ߣ޿߁᣿⏕ߥᴦ≮⋡ᮡ߇ሽ࿷ߔࠆὐ
߽㊀ⷐߢ޽ࠆ‫ޕ‬
RPE ߪ‫⚻␹ޔ‬ᕈ✂⤑ߣ⣂⛊⤑ⴊ▤ጀߣߩ㑆ߦ޽ࠅ‫⚻␹ޔ‬ᕈ✂⤑ߩઍ⻢߿↢ℂᯏ⢻ߦߣߞߡᰳ߆ߖߥ޿ᓎഀࠍᜂߞߡ޿ࠆ‫ޕ‬ᚑᾫߒߚ RPE ⚦⢩ߪ‫⃿⌒ޔ‬ౝߩ↢ℂ⊛ⅣႺਅߢߪಽⵚ
ߒߡ޿ߥ޿߇‫߇⤑✂ޔ‬ᄖ்ࠍⵍࠆߣჇᱺߒᕈ⾰ࠍᄌൻߐߖࠆ‫ޔߡ޿߅ߦ࠻ࡅޕ‬RPE ⚦⢩ߩჇᱺߪ‫ޔ‬ᄬ᣿ߦߟߥ߇ࠆჇᱺᕈ⎣ሶ૕✂⤑∝ߩళ୥ߢ޽ࠆ‫ޕ‬ౣ↢⢻ജߩ㜞޿ࠗࡕ࡝ߢ߽‫ޔ‬
RPE ⚦⢩ߪჇᱺߔࠆ߇‫ࠍ⤑✂ߦ⊛⚳ᦨޔ‬ౣ↢ߔࠆ‫ޔ߽ߢ࡝ࡕ߽ࠗߢ࠻ࡅޔߦ߁ࠃߩߎޕ‬RPE ⚦⢩ߪ✂⤑ߩ៊்ߦᔕߓߡჇᱺߔࠆ߇‫ߩߘޔ‬Ꮻ⚿߇ోߊ⇣ߥࠆ㧔ᄬ᣿߆ౣ↢㧕‫ߩߎޕ‬ߚ߼‫⤑✂ߩ࡝ࡕࠗޔ‬ౣ↢ߪ‫ߩ⤑✂ޔ‬ᄖ்ᕈ∔ᖚߩᴦ≮ߣౣ↢ߩߚ߼ߩၮ␆ᖱႎࠍᓧࠆࠃ޿
ࡕ࠺࡞ߢ߽޽ࠆ‫ޕ‬ Ⴧᱺᕈ⎣ሶ૕✂⤑∝ߩㆊ⒟ࠍฝ࿑ߦ␜ߔ‫߇⃿⌒ޕ‬੐᡿╬ߦࠃࠅᒝ޿ⴣ᠄߿ᄖ்
ࠍⵍࠆߣ‫߇❱⚵⚻␹⤑✂ޔ‬
RPE ߆ࠄ೸㔌㧔޿ࠊࠁࠆ✂⤑೸㔌㧕ߒ‫❱⚵⚻␹⤑✂ޔ‬ߦⵚญ߇↢ߓࠆ႐ว߇޽ࠆ
㧔A, B㧕‫ߩ❱⚵⚻␹⤑✂ޕ‬ౝㇱ߿๟࿐ߩⴊ▤߆ࠄᵹ಴ߒߚⴊᶧߦ RPE ߇᥸㔺ߐࠇࠆߣ‫ޔ‬
RPE ߪߎࠇߦᔕ╵ߒߡ਄⊹ᒻᘒࠍᄬ޿‫ޔ‬න㔌⁁ᘒߦߥߞߚ⚦⢩ߪၮᐩ⤑ Bruch̉smembrane ߆ࠄ㔌ࠇߡⵚญߦะߌߡㆆ⿛ߔࠆ㧔C㧕‫ޕ‬ㆆ⿛ㆊ⒟ߢ RPE ⚦⢩ߪ╭✢⛽⧘⚦⢩᭽ߩ⚦⢩ߦᒻ⾰ォ឵ߔࠆߣߣ߽ߦಽⵚ⢻ࠍ₪ᓧߔࠆ‫ޕ‬⚦⢩߇ⵚญߦઃ⌕ߔࠆߣ‫ޔ‬Ⴧᱺߒ‫ޔ‬✂⤑␹⚻⚵❱ਛߩࠣ࡝ࠕ⚦⢩╬ߣߣ߽ߦⵚญࠍⷒ߁⊹⤑ࠍᒻᚑߔࠆ㧔D, E㧕‫ߩߎޕ‬ㆊ⒟ߪ
৻⒳ߩഃ்ᴦ≹ㆊ⒟ߣ⠨߃ࠄࠇߡ޿ࠆ‫⊒ߩߘߪ⤑⊹ޔߒ߆ߒޕ‬㆐ߣߣ߽ߦ෼❗ߒ‫⚻␹ޔ‬ᕈ✂⤑ࠍᒁ߈ㄟ߻ߎߣߢ✂⤑೸㔌ࠍㅴⴕߐߖ‫⚿ޔ‬ᨐߣߒߡᄬ᣿ߦߟߥ߇ߞߡߒ߹߁㧔F㧕‫ޕ‬ߎߩࠃ߁ߦ‫ ࠻ࡅޔ‬RPE ⚦⢩ߪ✂⤑ߩᄖ்ߦᔕߓߡ㑆⪲⚦⢩᭽ߩ⚦⢩ߦᆫࠍᄌ߃㧔޿ࠊࠁࠆ਄⊹㑆⪲⒖ⴕ㧧EMT㧕‫ޔ‬Ⴧᱺߒ‫⚦ߩߎޔ‬⢩߇ේ࿃ߢᦨ⚳⊛ߦᄬ᣿ߦ⥋ࠆ‫ޕ‬ ฝߩ࿑ߪ‫ޔ‬ᚑ૕ࠗࡕ࡝✂⤑ౣ↢ߩᮨᑼ࿑ߢ޽ࠆ‫ߩߎޕ‬േ‛ߩ႐ว‫⌒ޔ‬⃿߆ࠄ✂⤑␹⚻⚵❱ࠍߔߴߡขࠅ෰
ߞߡ߽‫ޔ‬ᱷߞߚ RPE ⚦⢩߆ࠄ↢ℂ⊛ߥ✂⤑⚵❱㧔RPE ߣ✂⤑␹⚻⚵❱ߩਔᣇ㧕ࠍౣ↢ߢ߈ࠆ‫␹ࠄ߆⃿⌒ޕ‬⚻ᕈ✂⤑ࠍ㒰෰ߔࠆ㧔A, B㧕ߣ‫ޔ‬RPEߪࡅ࠻ߩ႐วߣห᭽ߦ਄⊹ᒻᘒࠍᄬ޿න㔌⚦⢩⁁ᘒߦߥࠆ㧔C㧕‫⚦ߩࠄࠇߎޕ‬⢩ߪ‫ޔ‬ಽⵚࠍ㐿ᆎߔࠆߣߣ߽ߦ‫৻ޔ‬᭽ߦ✂⤑ᐙ⚦⢩ߩࡑ࡯ࠞ࡯ㆮવሶ Pax6 ࠍ⊒⃻ߔࠆ㧔ᧂ౏⴫㧕‫ޕ‬ߘߩᓟ‫⚦ߩߎޔ‬⢩㓸࿅ߪ⦡⚛ࠍ଻ᜬߒߚ߹߹ 2 ጀߦಽ㔌ߒ‫ޔ‬ᄖጀߪ RPE ጀߦ‫ޔ‬ౝጀߪ✂⤑೨㚟⚦⢩ጀߣߥࠅ㧔D㧕‫ࠍ❱⚵⚻␹⤑✂ߦ⊛⚳ᦨޔ‬ౣ↢ߔࠆ㧔EψA㧕‫ࠗޔߦ߁ࠃߩߎޕ‬ࡕ࡝ߢ߽‫ޔ‬RPE ⚦⢩ߪ✂⤑ߩᄖ்ߦᔕߓߡ EMT ᭽ߩᄌൻࠍⵍࠅჇᱺߔࠆ߇‫ߩߎޔ‬Ⴧᱺ⚦⢩㧔⑳ߚߜߪߎߩ⚦⢩ࠍ Stem-like cells ߣ๭ࠎߢ޿ࠆ㧕߇‫ࠍ⤑✂ޔ‬ቢోౣ↢ߔࠆ⿠Ḯ⚦⢩ߦߥࠆߩߢ޽ࠆ‫ޕ‬ ᦨㄭߩ⎇ⓥ߆ࠄ‫ ߩ࠻ࡅޔ‬RPE ⚦⢩߽ᐙ⚦⢩㧔RPE stem-cell; RPESC㧕ߦᄌࠊࠅᓧࠆߎ
ߣ߇␜ߐࠇߡ޿ࠆ
㧔 Salero et al.,Cell Stem Cell
10: 88-95, 2012㧕‫ޕ‬ߒ߆ߒ‫⚦ߩߎޔ‬⢩ߪ‫⇣ߪߣ࡝ࡕࠗޔ‬ߥࠅ‫⚦⧘⛽✢╭ޔ‬⢩߿⢽⢌⚦⢩‫ޔ‬㛽߿エ㛽⚦⢩ߩᒻ⾰ࠍ⊒⃻ߔࠆߎߣߪߢ߈ࠆ߇‫⚦⚻␹⤑✂ޔ‬⢩ࠍ↢ߺ಴ߔ⢻ജߪૐ޿‫ߥߔޕ‬ࠊߜ‫ ࠻ࡅޔ‬RPESC ߪ‫ߥ߁ࠃߩ⤑✂ޔ‬ᄖ⢦⪲ᕈ⚵❱ߢߪߥߊ‫ޔ‬૗ࠄ߆ߩේ࿃ߢਛ⢦⪲ᕈߩ⚦⢩࡮⚵❱ࠍ↢ߺ಴ߒߡߒ߹߁ࠃ߁ߦ⷗߃ࠆ㧔೨㗁ߩਅ࿑㧕‫ޕ‬ࠗࡕ࡝ߣߩᲧセ߆ࠄ‫ߩߎޔ‬ὐߪ㕖Ᏹߦ⥝๧ᷓ޿‫ ߩ࠻ࡅޔߣࠆߔ߆ߒ߽ޕ‬RPE ⚦⢩ߪ‫ޔ‬ࠗࡕ࡝ߩࠃ߁ߥᱜᏱߥ㧔ౣ↢น⢻ߥ㧕ᐙ⚦⢩ࠍ↢ߺ಴ߖߥ޿ߩ߆߽ߒࠇߥ޿‫ޔߜࠊߥߔޕ‬ࡅ࠻߇✂⤑ࠍౣ↢ߢ߈ߥ޿ⷐ࿃ߩ৻ߟߦ‫ޟ‬ᐙ⚦⢩ߩᒻᚑ⇣Ᏹ޽ࠆ޿ߪᯏ⢻⇣Ᏹ‫ߩࠆ޽߇ޠ‬߆߽ߒࠇߥ޿‫⤑✂ޔ߫ࠇ޽ߢ߁ߘߒ߽ޕ‬ᄖ்ᓟߩ RPE ⚦⢩ߩ᜼േࠍಽሶ࡟ࡌ࡞ߢࠗࡕ࡝ߣᲧセߔࠆߎߣߦࠃߞߡ‫ߩߘޔ‬ᴦ≮߿ౣ↢⺃ዉߩࡅࡦ࠻߇ᓧࠄࠇࠆ߆߽ߒࠇߥ޿‫ޕ‬ዋߥߊߣ߽‫ޔ‬RPE ⚦⢩ߩಽⵚ㐿ᆎߦ޽ߚߞߡߪ‫ޔ߽࡝ࡕ߽ࠗ࠻ࡅޔ‬MAP ࠠ࠽࡯࠯⚻〝ߩ৻ߟߢ޽ࠆ MEK-ERK ⚻〝ߩᵴᕈ਄᣹ߣ‫⚦ޔ‬⢩㑆ធ⌕㧔޽ࠆ޿ߪធ⸅㒖ᱛ㧕߆ࠄߩ⸃᡼߇ᔅⷐࠄ
ߒ޿㧔Yoshikawa et al., Pigment Cell Melanoma Res. 25: 66-82, 2012; Mizuno et al.,Neurosci. Lett., 523: 39-44, 2012㧕‫ ࡝ࡕࠗޕ‬RPE ⚦⢩߇ᅤ૗ߦߒߡ✂⤑ࠍౣ↢ߔࠆᐙ⚦⢩ߦ࡝ࡊࡠࠣ࡜ࡓߐࠇࠆ߆߇੹ᓟߩὶὐߢ޽ࠆ‫ޕ‬ᦨㄭ‫ޔ‬ES㧛iPS ⚦⢩߆ࠄ in vitro ߢ RPE ߿␹⚻ᕈ✂⤑ࠍ↢ߺ಴ߒ‫ޔ‬⒖ᬀߦࠃࠅᄬߞߚ
ⷞⷡᯏ⢻ࠍขࠅᚯߔ⹜ߺ߇ㅴࠎߢ޿ࠆ‫ޔ߫߃଀ޕ‬ℂൻቇ⎇ⓥᚲ࡮⊒↢ౣ↢⑼ቇ✚ว⎇ⓥ࠮
ࡦ࠲࡯ߩ㜞ᯅ᡽ઍ᳁㧔✂⤑ౣ↢࡜ࡏ㧕߇ਛᔃߣߥߞߡ‫ޔ‬ട㦂㤛᢬ᄌᕈ∝ߩᴦ≮ߦะߌ iPS⚦⢩↱᧪ߩ RPE ⚵❱ࠍᖚ⠪ߩ⌒⃿ౝߦ⒖ᬀߔࠆ⥃ᐥ⹜㛎ࠍ㐿ᆎߒߚߎߣߪ๟⍮ߩߎߣߢ޽ࠆ‫ޔߚ߹ޕ‬ห⎇ⓥ࠮ࡦ࠲࡯ߩ╣੗⧐᮸᳁㧔ེቭ⊒↢࡜ࡏ㧕߇‫ޔ‬ES㧛iPS ⚦⢩߆ࠄ⌒⢩࡮⌒⋄‫ߺ↢ࠍ❱⚵⤑✂ߪߦࠄߐޔ‬಴ߔߎߣߦᚑഞߒ‫⃻ޔ‬࿷‫଻ߩߘޔ‬ሽᛛⴚߩ㐿⊒߿‫ޔ‬㜞ᯅ᳁ߣߩ౒หߢ‫⃿⌒ޔ‬ౝ߳ߩ⒖ᬀᛛⴚߩ㐿⊒ࠍㅴ߼ߡ޿ࠆߎߣ߽๟⍮ߩߣ߅ࠅߢ޽ࠆ‫ߒ߁ߎޕ‬ߚ ES㧛iPS ⚦⢩߆ࠄߩ⚦⢩࡮⚵❱ഃ⵾ᛛⴚߪ‫‛↢↢⊒ޔ‬ቇߩ⍮⷗ࠍ✚േຬߒߚ߽ߩߢ޽ࠅ‫ޔ‬ߎߩቇ໧ಽ㊁ߩ⽸₂ᐲߪ㕖Ᏹߦ㜞޿‫ޕ‬৻ᣇ‫ߩߢ߹ࠇߎߚ޿↪ࠍ࡝ࡕࠗޔ‬ౣ↢⎇ⓥ߆ࠄ‫ߩߎޔ‬േ‛߇⊒↢ߣߪ⇣ߥࠆ߿ࠅᣇߢ✂⤑᭴ㅧࠍ↢ߺ಴ߒߡ޿ࠆߎߣߪ᣿ࠄ߆ߢ޽ࠆ‫ޔߪ࡝ࡕࠗޔߜࠊߥߔޕ‬RPE ߣ޿߁ᚑᾫߒߚ
න৻ߩ⚦⢩ࠪ࡯࠻߆ࠄ‫ޔ‬EMT ߩࡊࡠ࠮ࠬࠍ੺ߒߡᐙ⚦⢩ࠍ↢ߺ಴ߒ‫⚻␹ޔ‬ᕈ✂⤑ߣ RPEߩ㧟ᰴర᭴ㅧࠍ߹ࠆߞߣ૞ࠅ਄ߍࠆߩߢ޽ࠆ‫ߩߎޕ‬ᚢ⇛ࠍណ↪ߔࠆߎߣߢ‫ߪ࡝ࡕࠗޔ‬ᄬߞߚ⚵❱ߣหߓᄢ߈ߐߩ⚵❱ࠍ⍴ᦼ㑆ߦౣ↢ߐߖࠆߎߣߦᚑഞߒߡ޿ࠆ‫ޔ߽߆ߒޕ‬ᄖ்ࠍⵍߞߚ⌒⃿ౝߢ‫ߔ␜ߩ࡝ࡕࠗߚߒ߁ߎޕࠆ޽ߢޔ‬ౣ↢ේℂࠍℂ⸃ߒ‫ޔ‬ක≮ߦᔕ↪ߢ߈ࠇ߫‫ޔ‬⒖ᬀౣ↢ߦߖࠃ in vivo ౣ↢ߦߖࠃ‫ޔ‬ᰴᰴ਎ઍဳߩ߽߁৻ߟߩ✂⤑ౣ↢ᛛⴚߦߟߥ߇ࠆߣ
ᦼᓙߢ߈ࠆ‫ޕ‬✂⤑ౣ↢ߛߌߢߪߥ޿‫⚦ޕ‬⢩㑆ߩ⚿ว߿ㅪ⛊ࠍ⸃ߊ EMT ߣห᭽ߩ⃻⽎ߪ‫ޔ‬ᔃ⤳߿྾⢇ߩౣ↢ߢ߽ⷰኤߐࠇࠆ‫ ߩ࡝ࡕࠗ߇ࠬ࠮ࡠࡊߩߎޔࠄ߆ߣߎߩߎޕ‬body-parts ౣ↢ߔߴߡߦᔅⷐਇนᰳߥࡔࠞ࠾࠭ࡓߣߒߡ㑐ࠊߞߡ޿ࠆน⢻ᕈ߇⠨߃ࠄࠇ‫⤑✂ޔ‬ౣ↢⎇ⓥ߆ࠄߩ⍮⷗߇ઁߩ⚵❱࡮ེቭߩౣ↢⎇ⓥߦᵄ෸ߔࠆߎߣ߽චಽߦ੍ᗐߐࠇࠆ‫ޕ‬એ਄ߩࠃ߁ߦ‫⤑✂࡝ࡕࠗޔ߇ࠆ޽ߪߢࡠࠢࡑߛᧂޔߪߦߜߚ⑳ޔ‬ౣ↢ߣࡅ࠻ᄖ்ᕈ✂⤑∔ᖚ㧔଀߃߫‫ޔ‬Ⴧᱺᕈ⎣ሶ૕✂⤑∝㧕ߩ౒ㅢὐߣ⋧㆑ὐ߇⷗߃ߡ߈ߡ޿ࠆ‫⃻ޕ‬࿷‫ࠄࠇߎޔ‬ߩಽሶၮ⋚ࠍ᣿ࠄ߆ߦߔࠆߚ߼ߦ‫ࠢ࠶࠾ࠚࠫࠬࡦ࡜࠻ޔ‬ᛛⴚࠍ㚟૶ߒߚන৻ RPE ⚦⢩࡟ࡌ࡞ߩォ౮⸃ᨆ߅ࠃ߮ㆮવሶᯏ⢻⸃ᨆࠍᆎ߼ߡ޿ࠆ‫⎇ߩߎޕ‬ⓥߩㅴዷߩߚ߼ߦ‫ޔ‬JNRC ߦࠃࠆ⾗Ḯ࡮ᛛⴚ࡮ᖱႎߪߘߩၮ⋚ߣߒߡ⋉‫ޘ‬㊀ⷐᕈ߇㜞߹ߞߡ޿ࠆߣ⸒߃ࠆ‫ޕ‬㨇ߎࠇࠄߩౝኈߩ৻ㇱߪ‫ޔ‬Experimental Eye Research ߦ✚⺑ߣߒߡឝタߐࠇࠆ੍ቯߢߔ㨉࠯ࡉ࡜ࡈࠖ࠶ࠪࡘߩ⦡⚛⚦⢩㑆⋧੕૞↪ߣࡄ࠲࡯ࡦᒻᚑ
ᷰㆺᱜൎ 㧔㒋ᄢ࡮↢๮ᯏ⢻㧕ߪߓ߼ߦ  േ‛ߩᜬߟ૕⴫ᮨ᭽ߪ‫ޔ‬᝝㘩⠪߆ࠄߩㅏㆱ߿ห⒳㑆ߩ⹺⼂‫↢ޔ‬ᱺᚢ⇛ߥߤ‫ޔ‬⒳ߩ
↢ሽߦ㊀ⷐߥᓎഀࠍᜬߞߡ޿ࠆߣ⠨߃ࠄࠆ‫ߩߎޔߡߒߘޕ‬૕⴫ᮨ᭽ߦ㑐ߒߡᵈ⋡ߔߴ߈ὐߣߒߡ‫ޔ‬ㄭ✼⒳߇ోߊ⇣ߥࠆ૕⴫ᮨ᭽ࠍ␜ߒߚࠅ‫৻ޔ‬ᣇߢ♽⛔⊛ߦోߊ⇣ߥࠆ↢‛⒳߇㘃ૃߥᮨ᭽ࠍ␜ߔߣ޿߁੐⽎߇޽ߍࠄࠇࠆ‫࠻࠶ࡐࠬޕ‬ᮨ᭽߿ࠬ࠻࡜ࠗࡊᮨ᭽߇
㝼㘃߆ࠄູ੃㘃ߦ⥋ࠆ߹ߢ᭽‫ߥޘ‬േ‛ߢߺࠄࠇࠆߎߣ߇৻଀ߢ޽ࠆ‫ߩߎޕ‬੐ታ߆ࠄ‫ޔ‬േ‛ߩ૕⴫ᮨ᭽ߪߘߩᒻᚑߦ߆߆ࠊࠆ⚦⢩߿ಽሶ‫ޔ‬ㆮવሶߪ㆑ߞߡ޿ߡ߽౒ㅢߩේℂ
ߩ߽ߣߦᚑࠅ┙ߞߡ޿ࠆ‫߇ߣߎ߁޿ߣޔ‬⠨߃ࠄࠆ‫ޕ‬ᚒ‫ߩ‛↢ޔߪߢࡊ࡯࡞ࠣߩޘ‬ᒻᘒᒻᚑߩਛߢ߽‫⚦ߦ․ޔ‬⢩⥄ᓞ⊛ߥࡄ࠲࡯ࡦᒻᚑߣ޿߁⃻⽎ߦᵈ⋡ߒಽሶᯏ᭴ߩℂ⸃ࠍ⋡ᜰߒ⎇ⓥࠍⴕߞߡ޿ࠆ‫ߩߎޕ‬໧㗴ߦኻߒߡ‫ޔ‬ᚒ‫⎇ߪޘ‬ⓥ᧚ᢱߣߒߡ࠯ࡉ࡜ࡈࠖ࠶ࠪ
ࡘࠍ↪޿ߡ޿ࠆ‫↢⊒ߪࡘࠪ࠶ࠖࡈ࡜ࡉ࠯ޔߪࠇߎޕ‬ቇ‫ޔ‬ㆮવቇߩࡕ࠺࡞↢‛ߢ޽ࠅ‫ޔ‬ࠥࡁࡓ⸃⺒߽⚳ੌߒߡ޿ࠆὐ‫ޔ‬ㆮવሶ⚵ߺ឵߃૕ߩ૞⵾߽ኈᤃߦⴕ߃ࠆὐ‫ߡߒߘޔ‬૗ࠃࠅࠬ࠻࡜ࠗࡊᮨ᭽ࠍᜬߟὐ߇ᄢ߈ߥℂ↱ߢ޽ࠆ‫ޕ‬࠯ࡉ࡜ࡈࠖ࠶ࠪࡘߩࠬ࠻࡜ࠗࡊ  ࠯ࡉ࡜ࡈࠖ࠶ࠪࡘ㧔࿑ 1A㧕ߦߪ 3 ⒳㘃ߩ⦡⚛⢩߇ሽ࿷ߔࠆ㧔㝼㘃ߩ⦡⚛⚦⢩ߪ⦡⚛㗰☸ߩ᡼಴ߪⴕࠊߥ޿ߚ߼‫੃ູޔ‬㘃ߩ⦡⚛⚦⢩ߣߪಽߌߡ⦡⚛⢩ߣ๭߫ࠇࠆ㧕‫ߩߘޕ‬ਛߢ㤥⦡⚛⢩ߣ㤛⦡⚛⢩߇޿ࠊࠁࠆࠬ࠻࡜ࠗࡊࡄ࠲࡯ࡦࠍᒻᚑߔࠆ࿃ሶߢ޽ࠆ‫ߩߎޕ‬߶߆ߦ㌁⦡ߩశᴛࠍ߽ߟ⦡⚛⚦⢩߇ሽ࿷ߒⰂ⦡⚛⢩ߣ๭߫ࠇߡ޿ࠆ‫⚦⚛⦡ޕ‬⢩ߪ޿ߕࠇ߽␹⚻ႇ⚦⢩↱᧪ߩ⚦⢩ߢ‫ߩࡘࠪ࠶ࠖࡈ࡜ࡉ࠯ޔ‬୘૕⊒↢ߦ઻޿ಽൻ࡮⊒↢࡮⒖േ
ࠍⴕ޿૕⴫ᮨ᭽ࠍᒻ૞ࠆ‫ޕ‬૕ᐙㇱߩࠬ࠻࡜ࠗࡊߦᵈ⋡ߔࠆߣ‫ߕ߹ޔ‬ᱜਛ✢ߦᴪߞߡ‫ޔ‬㤥⦡⚛⢩߇৻೉ߦਗࠎߛ✢߇ߢ߈‫ߩߘޔ‬ᓟ‫ߩߎޔ‬㤥⦡⚛⢩ߩ✢ߦ㊀ߥࠆᒻߢ㤛⦡⚛⢩߇⃻ࠇ‫ޔ‬หᤨߦ㤥⦡⚛⢩ߪ㤛⦡⚛⢩߆ࠄㅏߍࠆᒻߢ⢛஥޽ࠆ޿ߪ⣻஥ߦ⒖േߔࠆ‫ߎޕ‬ߩᤨ‫ޔ‬㤛⦡⚛⢩߆ࠄㅏߍ߈ࠇߥ߆ߞߚ㤥⦡⚛⢩ߪ⚦⢩ᱫࠍ⿠ߎߔ‫⇣ߥ߁ࠃߩߎޕ‬⒳⚦⢩㑆ߩ෻⊒૞↪ࠍ઻߁⚦⢩⒖േߦࠃࠅ‫ޔ‬㤛⦡⚛⢩‫ޔ‬㤥⦡⚛⢩ߩ㗔ၞಽߌ߇ߥߐࠇ‫ࠬޔ‬࠻࡜ࠗࡊࡄ࠲࡯ࡦ߇ቢᚑߔࠆ 1‫ޕ‬࠯ࡉ࡜ࡈࠖ࠶ࠪࡘᮨ᭽ᄌ⇣૕  ࠯ࡉ࡜ࡈࠖ࠶ࠪࡘ߆ࠄߪ᭽‫ߥޘ‬ᄌ⇣૕߇න㔌ߐࠇㆮવሶᯏ⢻ߩ⸃ᨆߦ↪޿ࠄࠇߡ߈ߚ‫ޕ‬ᄙߊߩ႐ว‫ޔ‬ENU ᄌ⇣ේߦࠃࠅ૞⵾ߐࠇߚᄌ⇣૕ߢ޽ࠅ‫ߩߎޔ‬ਛߦߪ૕⴫ᮨ᭽
ᄌ⇣૕߽฽߹ࠇࠆ‫ ࠄ߆੹ޔߢࠈߎߣޕ‬10 ᐕ⒟೨߹ߢߩ૕⴫ᮨ᭽ᄌ⇣૕ߩ⸃ᨆߪ‫⚛⦡ޔ‬⚦⢩ߩಽൻ࡮⊒↢ᄌ⇣૕ߩ⸃ᨆ߇ਥߥ߽ߩߢ޽ࠅ‫ޔ‬න㔌ߐࠇߚㆮવሶߪູ੃㘃⦡⚛⚦⢩ߢ⸃ᨆ߇ㅴ߼ࠄࠇߡ޿ࠆㆮવሶߩࡎࡕࡠࠣߢ޽ࠆߎߣ߇ᄙ‫ ߚߒ߹ࠇࠄ⷗ޘ‬2; 3; 4; 5; 6; 7;
8; 9‫ߦࠇߎޕ‬ኻߒߡᚒ‫ޔߪߢࡊ࡯࡞ࠣߩޘ‬૕⴫ᮨ᭽ᄌ⇣૕ߩਛߢ߽‫ࡦ࡯࠲ࡄޔ‬ᄌ⇣૕ߦᵈ⋡ߒ⸃ᨆࠍⴕߞߡ߈ߚ‫⚦⚛⦡ࠅ߹ߟޕ‬⢩ߩ⊒↢࡮ಽൻߪ߶ߣࠎߤᱜᏱߛ߇‫ߩߘޔ‬㈩⟎߇㊁↢ဳߣ⇣ߥࠆ‫߁޿ߣޔ‬ᄌ⇣૕ߩ⸃ᨆߢ޽ࠆ‫ࠍ଀ޕ‬᜼ߍࠆߣ‫ޔ‬㊁↢ဳߩࠬ࠻࡜ࠗ
ࡊ߇ࠬࡐ࠶࠻ߦߥࠆ leopard ᄌ⇣૕ 10 ߿ seurat ᄌ⇣૕ 11‫ޔ‬㤛⦡⚛⢩ߣ㤥⦡⚛⢩߇ᷙߑࠅว߁ jaguar ᄌ⇣૕ 12 ߿ dali ᄌ⇣૕ߥߤߢ޽ࠆ߇‫੹ޔ‬࿁ߪ leopard ᄌ⇣૕㧔࿑ 1B㧕
ߣ jaguar ᄌ⇣૕㧔࿑ 1C㧕ߦߟ޿ߡ⚫੺ߔࠆ‫ޕ‬

leopard ᄌ⇣૕  leopard ᄌ⇣૕ߪ‫ޔ‬㊁↢ဳߩࠬ࠻࡜ࠗࡊ߇ࠬࡐ࠶࠻ߦߥࠆᄌ⇣૕ߢ‫ޔ‬㊁ᄖߢណ㓸ߐࠇߚᄌ⇣૕ߢ޽ࠅ‫ޔ‬ណขߐࠇߚᒰೋߪ࠯ࡉ࡜ࡈࠖ࠶ࠪࡘߣߪ೎⒳ߩ㝼ߛߣ⠨߃ࠄࠇߡ޿ߚߎߣߪ᦭ฬߢ޽ࠆ‫⚦ߪࡦ࡯࠲ࡄ࠻࠶ࡐࠬߩߎޔߡߒߘޕ‬⢩⥄ᓞ⊛ߥࡄ࠲࡯ࡦᒻ
ᚑߩࡕ࠺࡞ࠤ࡯ࠬߦߥࠅ߁ࠆߣ޿߁ߎߣ߇ᄙߊ⼏⺰ߐࠇߡ߈ߚ 13; 14; 15‫࡞࠽࡚ࠪࠫࡐޕ‬ࠢࡠ࡯࠾ࡦࠣࠍⴕ޿ㆮવሶߩ․ቯࠍⴕߞߚߣߎࠈ‫ߩࡦ࡚ࠪࠢࡦࡖࠫࡊ࠶ࡖࠡޔ‬᭴ᚑ࿃ሶߩ߭ߣߟߢ޽ࠆࠦࡀࠠࠪࡦ 41.8 ߣ޿߁ㆮવሶߦᄌ⇣߇⷗ߟ߆ߞߚ‫ޕ‬3 ߟ޽ࠆࠕ࡝࡞
ߣ߽ߎߩㆮવሶߦᄌ⇣߇ሽ࿷ߒ‫ޔ‬ഠᕈߩࠕ࡝࡞ߦߪ࠽ࡦ࠮ࡦࠬᄌ⇣߇‫ޔ‬ఝᕈߩࠕ࡝࡞
ߦߪࡒࠬ࠮ࡦࠬᄌ⇣߇ሽ࿷ߒߚ 10‫ޕ‬  ᰴߦࡄ࠲࡯ࡦᒻᚑߦ߅ߌࠆࠡࡖ࠶ࡊ⚿วߩᓎഀࠍᬌ⸛ߒߚ‫ࡖࠡޔࠄ߇ߥߒ߆ߒޕ‬࠶ࡊ⚿วߪㅢㆊߔࠆಽሶߩㆬᛯᕈ߇ૐ޿ߚ߼‫ߪߡ޿ߟߦࡓ࠭࠾ࠞࡔߥ⚦⹦ޔ‬ᱷᔨߥ߇ࠄ߹ߛ᣿ࠄ߆ߦߪߥߞߡ޿ߥ޿‫੃ູޔߢࠈߎߣޕ‬㘃ߦߪ⚂ 20 ⒳㘃‫ޔ‬㝼㘃ߦߪ⚂ 40 ⒳㘃ߩࠦࡀࠠࠪࡦㆮવሶ߇ሽ࿷ߔࠆ‫ߩࡦࠪࠠࡀࠦߩࠄࠇߎޕ‬ᯏ⢻ߩᏅ߆ࠄ૗߆ࡅࡦ࠻ߪ
ᓧࠄࠇߥ޿߽ߩ߆ߣ⠨߃‫ޔ‬40 ⒳㘃ߩਛߩ޿ߊߟ߆ߩㆮવሶࠍ↪޿ߡ leopard ᄌ⇣૕ߩ⋧⵬ታ㛎ࠍⴕߞߚ‫⚿ߩߘޕ‬ᨐ‫ ࡦࠪࠠࡀࠦޔ‬41.8 ߦㄭ✼ߥ৻⟲ߩㆮવሶߩߺ߇⴫⃻ဳࠍ࡟ࠬࠠࡘ࡯ߢ߈ࠆߎߣ߇ࠊ߆ߞߚ㧔࿑㧞㧕‫ߩࠄࠇߎޕ‬ㆮવሶ߇ࠦ࡯࠼ߔࠆⰮ⊕⾰ߩࠕࡒ
ࡁ㉄㈩೉ࠍᲧセߒߚߣߎࠈ N ᧃ㗔ၞߦ ExxxE ߣ޿߁ࡕ࠴࡯ࡈ߇ሽ࿷ߒߚ㧔࿑㧟㧕‫ߎޕ‬ߩ㗔ၞߪࡐ࡝ࠕࡒࡦ߇⚿วߒ‫⚿ࡊ࠶ࡖࠡޔ‬วߦᢛᵹᕈࠍਈ߃ࠆߎߣ߇‫ޔ‬in vitro ߩታ㛎ߦࠃࠅ⏕߆߼ࠄࠇߡ޿ࠆࡕ࠴࡯ࡈߢ‫ߩߎޔ‬㈩೉ࠍ⟎឵ߒߚ႐ว‫ޔ‬ᄌ⇣ဳࠦࡀࠠࠪࡦߪ
leopard ⴫⃻ဳࠍ࡟ࠬࠠࡘ࡯ߢ߈ߥ߆ߞߚ㧔࿑㧟㧕‫⚦⚛⦡ޔߪߣߎߩߎޕ‬⢩㑆ߦᒻᚑߐࠇࠆࠡࡖ࠶ࡊ⚿วߦᣇะᕈߩ޽ࠆᵹࠇ߇ሽ࿷ߒ‫ࡦ࡯࠲ࡄ߇ࠇߎޔ‬ᒻᚑߦ㊀ⷐߥᗧ๧ࠍ
ᜬߟߣ޿߁ߎߣࠍ␜ໂߒߡ޿ࠆ 16‫ޕ‬  ࠦࡀࠠࠪࡦߩ N ᧃ㈩೉߇ࡄ࠲࡯ࡦᒻᚑߦ㊀ⷐߛߣ޿߁ߎߣ߆ࠄ‫ߩߎޔ‬㗔ၞߦ޿ߊߟ߆ߩᄌ⇣ࠍዉ౉ߒߚࠦࡀࠠࠪࡦࠍ૞⵾ߒ‫ߦࡘࠪ࠶ࠖࡈ࡜ࡉ࠯ޔ‬ዉ౉ߔࠆታ㛎ࠍⴕߞߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬㧟ࠕࡒࡁ㉄ࠍ૛⸘ߦઃടߒߚᄌ⇣ဳࠦࡀࠠࠪࡦߢߪ‫ޔ‬㊁↢ဳߩࠬ࠻࡜
ࠗࡊ߇ᚑ㐳ߣߣ߽ߦഀࠇ‫⚿ޔ‬ᨐߣߒߡ⚦޿ࠬ࠻࡜ࠗࡊ߇಴᧪਄߇ߞߚ‫৻ޕ‬ᣇ‫ޔ‬6 ࠕࡒࡁ㉄⍴ߊߒߚ႐ว‫ޔ‬ਇᕁ⼏ߥߎߣߦㅅችᮨ᭽߇⃻ࠇߚ 17‫ޕ‬jaguar ᄌ⇣૕ jaguar ᄌ⇣૕ߪ㤛⦡⚛⢩ߣ㤥⦡⚛⢩߇૕⴫਄ߢᷙߑࠅวߞߡߒ߹߁ᄌ⇣૕ߢ޽ࠆ‫ޕ‬ߎߩᄌ⇣૕ߦߟ޿ߡߪᄢቇ㒮↢ߩጤਅ߇ㆮવሶߩන㔌ࠍⴕ޿ 12‫ޔ‬หߓߊᄢቇ㒮↢ߩⒷ⪲߇⚦⢩㑆⋧੕૞↪ߦ߅ߌࠆᯏ⢻ߩ⸃᣿ࠍⴕߞߚߩߢߎߎߢ⚫੺ߔࠆ 18‫ ޕ‬leopard ᄌ⇣૕ߣห᭽ߦࡐ࡚ࠫࠪ࠽࡞ࠢࡠ࡯࠾ࡦࠣߦࠃࠅේ࿃ㆮવሶߩ․ቯࠍⴕ޿‫ޔ‬ౝะ߈ᢛᵹ
ᕈࠞ࡝࠙ࡓ࠴ࡖࡀ࡞ߩ৻⒳‫ޔ‬Kir7.1 ߦᄌ⇣߇ᬌ಴ߐࠇߚ‫ޔߡߒߘޕ‬Kir7.1 ਄ߦ⿠ߎߞ
ߚࠕࡒࡁ㉄⟎឵ߦࠃࠅ‫ߤࠎߣ߶ࠍࡦࠝࠗࡓ࠙࡝ࠞޔ‬ㅢߐߥߊߥߞߡ޿ߚ‫ޕ‬ౝะ߈ᢛᵹ
ᕈࠞ࡝࠙ࡓ࠴ࡖࡀ࡞ߪ‫⚦ޔ‬⢩ߩ㕒ᱛ⤑㔚૏ߩᒻᚑࠍⴕ޿‫ߩߎޔߚ߹ޔ‬ౝะ߈ᢛᵹᕈߪ
⚦⢩ౝ࠼ࡔࠗࡦߦࡐ࡝ࠕࡒࡦ߇⚿วߔࠆߎߣߦࠃࠅᒻᚑߐࠇࠆߎߣ߇⍮ࠄࠇߡ޿ࠆ‫ޕ‬ߎߩ࠴ࡖࡀ࡞߇უࠇࠆߎߣߦࠃߞߡ‫ޔ‬㤥⦡⚛⢩ߪᏱߦ⣕ಽᭂߒߚ⁁ᘒߦߥߞߡ޿ߚ‫ޕ‬ߣߎࠈߢ‫ޔ‬਄ㅀߩ㤛⦡⚛⢩́㤥⦡⚛⢩㑆ߩ⋧੕૞↪ࠍ⤑㔚૏ᗵฃᕈ⦡⚛ࠍ↪޿ߡ invitro ၭ㙃♽ߢⷰኤߒߚߣߎࠈ‫ޔ‬㤛⦡⚛⢩߇㤥⦡⚛⢩ߦធ⸅ߒߚߣ߈ߦ㤥⦡⚛⢩ߩ⣕ಽᭂ߇⿠ߎߞߡ޿ߚ‫ߩߎޔߡߒߘޕ‬⣕ಽᭂ߇ᒁ߈㊄ߣߥߞߡ‫ޔ‬㤥⦡⚛⢩ߩ㤛⦡⚛⢩߆ࠄߩㅏㆱⴕേ߇ᒁ߈⿠ߎߐࠇߡ޿ߚ‫ޕ‬Ᏹߦ⣕ಽᭂ⁁ᘒߦ޽ࠆ jaguar ᄌ⇣૕↱᧪ߩ㤥⦡⚛
⢩ߢߪㅏㆱⴕേߪ⿠ߎࠄߕ‫⚿ߩߎޔ‬ᨐߣߒߡ‫ޔ‬૕⴫਄ߢ㤛⦡⚛⢩ߣ㤥⦡⚛⢩߇ᷙߑࠅว߁ߣ޿߁⴫⃻ဳߣߥࠆߣ޿߁ߎߣ߇᣿ࠄ߆ߣߥߞߚ‫ޕ‬㔚ሶ㗼ᓸ㏜⸃ᨆ  ߣߎࠈߢ‫ޔ‬਄ㅀߩࠃ߁ߥ⋧੕૞↪߇⿠ߎࠆ႐ว‫ޔ‬㤛⦡⚛⢩́㤥⦡⚛⢩㑆ߦߪ⋥ធ⊛ߥធ⸅߇⿠ߎߞߡ޿ࠆߎߣ߇ᔅⷐߢ޽ࠆ‫ ࠄ߇ߥߒ߆ߒޕ‬2003 ᐕߦႎ๔ߐࠇߚ࠯ࡉ
࡜ࡈࠖ࠶ࠪࡘ૕⴫਄ߦ߅ߌࠆ㤛⦡⚛⢩ߣ㤥⦡⚛⢩ߩ㈩⟎ߦ㑐ߔࠆ㔚ሶ㗼ᓸ㏜⸃ᨆߢߪ‫ޔ‬ߎࠇࠄ⦡⚛⢩ߩ㑆ߦⰂ⦡⚛⢩ߩጀ߇޽ࠅ‫ޔ‬ਔ⠪ࠍ㓒ߡߡ޿ߚ‫⚿ߩߎޕ‬ᨐ‫⚦ޔ‬⢩㑆⋧੕૞↪߇ߤߩࠃ߁ߥᒻߢⴕࠊࠇߡ޿ࠆߩ߆ߪߪߞ߈ࠅߒߥ߆ߞߚ‫ޔߢߎߘޕ‬ᄢ㒋ᄢቇ⿥㜞࿶㔚ሶ㗼ᓸ㏜࠮ࡦ࠲࡯⷏↰୶Ꮧඳ჻ߩഥߌࠍ୫ࠅߡ‫⚛⦡ޔ‬⢩ߩ⸃ᨆࠍⴕߞߚ‫ߩߘޕ‬⚿ᨐ‫ޔ‬㤥⦡⚛⢩߇㕖Ᏹߦ㐳޿઒⿷ࠍિ߫ߒ‫ޔ‬㤛⦡⚛⢩ߦធ⸅ߒߡ޿ࠆ᭽ሶ߇᣿ࠄ߆ߦ
ߥߞߚ㧔࿑㧠㧕‫ޕ‬෻ᔕ᜛ᢔࡕ࠺࡞
  2 ߟߩ࿃ሶ߇⋧੕૞↪ߦࠃࠅ‫ޔ‬ⓨ㑆ࡄ࠲࡯ࡦࠍ૞ࠆߣ޿߁ᢙℂࡕ࠺࡞߇‫ࠄ߆੹ޔ‬⚂ 60 ᐕ೨ߦࠗࠡ࡝ࠬߩᢙቇ⠪ߢ޽ࠆࠕ࡜ࡦ࡮࠴ࡘ࡯࡝ࡦࠣߦࠃࠅឭ໒ߐࠇߡ޿ࠆ 19‫ޕ‬ᧄ⎇ⓥߢߪ 2 ߟߩ࿃ሶࠍ 2 ߟߩ⚦⢩‫ޔ‬㤛⦡⚛⢩ߣ㤥⦡⚛⢩ߦ⟎߈឵߃‫⋧ߩߘޔ‬੕૞↪
ࠍតߞߡ߈ߚ㧔࿑㧡㧕‫ޔࠈߎߣߩߢ߹ࠇߎޕ‬ਔ࿃ሶ㧔⚦⢩㧕㑆ߦሽ࿷ߔࠆᛥ೙ᕈߩ⋧੕૞↪߇ Kir7.1 ߦࠃࠆ߽ߩߢ޽ࠆߎߣ߇᣿ࠄ߆ߦߥߞߚ‫ޔߚ߹ޕ‬㤛⦡⚛⢩߆ࠄ㤥⦡⚛⢩߳ߩᵴᕈൻߦ㑐ߒߡ‫⏕ߛ߹ߪߡ޿ߟߦࠇߎޔ‬ቯߒߡߪ޿ߥ޿߇‫ޔ‬㐳޿઒⿷਄ߦᒻᚑߐ
ࠇࠆࠡࡖ࠶ࡊ⚿วߩᜬߟᢛᵹᕈߦࠃࠆ߽ߩߢߥ޿߆ߣ␜ໂߐࠇࠆ⚿ᨐ߇ᓧࠄࠇߚ‫ޕ‬਄ㅀߒߚ૕⴫਄ߦ᭽‫ߥޘ‬ᮨ᭽ࠍ૞ࠆ⃻⽎‫߇ࡊࠗ࡜࠻ࠬߦ․ޔ‬ഀࠇࠆ⃻⽎ߦߟ޿ߡߪ‫ࠡޔ‬ࡖ࠶ࡊ⚿วࠍ੺ߒߚ‫ޔ‬㤛⦡⚛⢩߆ࠄ㤥⦡⚛⢩߳ߩᵴᕈൻࠪࠣ࠽࡞ߩᷫዋߦ⿠࿃ߔࠆߩ
ߢߪߥ޿߆ߣ⠨߃ࠄࠇࠆ‫੹ޕ‬ᓟߪ‫ߩߎޔ‬૞↪࡮⴫⃻ဳࠍᚻ߇߆ࠅߦ‫⚿ࡊ࠶ࡖࠡޔ‬วࠍ੺ߒߚࠪࠣ࠽࡞ߩታᘒ⸃᣿ࠍⴕ޿ߚ޿ߣ⠨߃ߡ޿ࠆ‫ޔߢࠈߎߣޕ‬ᮨ᭽߇ഀࠇࠆ⃻⽎ߦߟ޿ߡߪ‫ޔ‬ೋᦼ᧦ઙࠍࠬ࠻࡜ࠗࡊߢߪߥߊࠬࡐ࠶࠻‫ߩ࠻࠶ࡐࠬޔࠅ߹ߟޔ‬㝼ߦഀࠇࠆㆮવሶࠍዉ౉ߔࠆߎߣߦࠃࠅ‫࡚࠙ࡅޔ‬ᨩࠍ૞ࠆߎߣ߇ߢ߈ࠆ㧔࿑㧢‫ޔ‬㧣㧕‫ޔߪ⽎⃻ߩߎޕ‬߹ߐߦ෻ᔕ᜛ᢔࡕ࠺࡞ߢ੍ᗐߐࠇߚㅢࠅߩ⚿ᨐߢ޽ࠆ‫ޕ‬߹ߣ߼  ࠯ࡉ࡜ࡈࠖ࠶ࠪࡘߩࠬ࠻࡜ࠗࡊᮨ᭽ࠍ㗴᧚ߦ⚦⢩⥄ᓞ⊛ߥࡄ࠲࡯ࡦᒻᚑߩಽሶᯏ
᭴ߩ⸃᣿ࠍ⋡ᜰߒߡ޿ࠆ‫ߞ޿ߣ࡞ࡀࡖ࠴ࡓ࠙࡝ࠞ߿ࡦࠪࠠࡀࠦޔࠈߎߣߩߢ߹ࠇߎޕ‬ߚ‫߇⾰ࠢࡄࡦ࠲⤑ޔ‬㊀ⷐߥᯏ⢻ࠍᜂߞߡ޿ࠆߎߣ߇᣿ࠄ߆ߣߥߞߚ‫ߩࠄࠇߎޕ‬ಽሶߪ‫ޔ‬߹ߐߦ‫ޔ‬෻ᔕ᜛ᢔࡕ࠺࡞ߩਛᩭࠍᜂ߁ಽሶߢ޽ࠆ‫ࠇߎޔߚ߹ޕ‬એᄖߦ߽⚦⢩㑆⋧੕૞↪ࠍᜂ߁ㆮવሶߩන㔌߇ㅴ߼ࠄࠇߡ߅ࠅ‫ޔ‬ㄭ޿߁ߜߦಽሶᯏ᭴ߩోኈ⸃᣿߇ߢ߈ࠆ߽

ߩߣᦼᓙߐࠇࠆ‫ޕ‬⻢ㄉ  ᧄ⎇ⓥߪᄢ㒋ᄢቇᄢቇ㒮↢๮ᯏ⢻⎇ⓥ⑼ㄭ⮮ṑ⎇ⓥቶߢⴕࠊࠇ߹ߒߚ‫ޕ‬㔚ሶ㗼ᓸ
㏜⸃ᨆߪ‫ޔ‬ᄢ㒋ᄢቇ⿥㜞࿶㔚ሶ㗼ᓸ㏜࠮ࡦ࠲࡯⷏↰୶Ꮧඳ჻ߣߩ౒ห⎇ⓥߢߔ‫ޕ‬ᢥ₂1.

Kelsh, R. N., Harris, M. L., Colanesi, S. & Erickson, C. A. (2009). Stripes andbelly-spots -- a review of pigment cell morphogenesis in vertebrates. Semin Cell

Dev Biol 20, 90-104.
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Dutton, K. A., Pauliny, A., Lopes, S. S., Elworthy, S., Carney, T. J., Rauch, J.,
Geisler, R., Haffter, P. & Kelsh, R. N. (2001). Zebrafish colourless encodes sox10and specifies non-ectomesenchymal neural crest fates. Development 128,
4113-25.

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Parichy, D. M., Mellgren, E. M., Rawls, J. F., Lopes, S. S., Kelsh, R. N. &Johnson, S. L. (2000). Mutational analysis of endothelin receptor b1 (rose)
during neural crest and pigment pattern development in the zebrafish Daniorerio. Dev Biol 227, 294-306.

4.

Parichy, D. M., Ransom, D. G., Paw, B., Zon, L. I. & Johnson, S. L. (2000). An
orthologue of the kit-related gene fms is required for development of neuralcrest-derived xanthophores and a subpopulation of adult melanocytes in thezebrafish, Danio rerio. Development 127, 3031-44.

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Parichy, D. M., Rawls, J. F., Pratt, S. J., Whitfield, T. T. & Johnson, S. L. (1999).
Zebrafish sparse corresponds to an orthologue of c-kit and is required for themorphogenesis of a subpopulation of melanocytes, but is not essential forhematopoiesis or primordial germ cell development. Development 126, 3425-36.

6.

Parichy, D. M. & Turner, J. M. (2003). Temporal and cellular requirements for
Fms signaling during zebrafish adult pigment pattern development.

Development 130, 817-33.
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Rawls, J. F. & Johnson, S. L. (2001). Requirements for the kit receptor tyrosine

kinase during regeneration of zebrafish fin melanocytes. Development 128,
1943-9.
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Lister, J. A., Close, J. & Raible, D. W. (2001). Duplicate mitf genes in zebrafish:complementary expression and conservation of melanogenic potential. Dev Biol
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Lister, J. A., Robertson, C. P., Lepage, T., Johnson, S. L. & Raible, D. W. (1999).
nacre encodes a zebrafish microphthalmia-related protein that regulates
neural-crest-derived pigment cell fate. Development 126, 3757-67.

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Watanabe, M., Iwashita, M., Ishii, M., Kurachi, Y., Kawakami, A., Kondo, S. &Okada, N. (2006). Spot pattern of leopard Danio is caused by mutation in thezebrafish connexin41.8 gene. EMBO Rep 7, 893-7.

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Eom, D. S., Inoue, S., Patterson, L. B., Gordon, T. N., Slingwine, R., Kondo, S.,
Watanabe, M. & Parichy, D. M. (2012). Melanophore migration and survival
during zebrafish adult pigment stripe development require the immunoglobulin
superfamily adhesion molecule Igsf11. PLoS Genet 8, e1002899.

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Iwashita, M., Watanabe, M., Ishii, M., Chen, T., Johnson, S. L., Kurachi, Y.,
Okada, N. & Kondo, S. (2006). Pigment pattern in jaguar/obelix zebrafish iscaused by a Kir7.1 mutation: implications for the regulation of melanosome
movement. PLoS Genet 2, e197.

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Asai, R., Taguchi, E., Kume, Y., Saito, M. & Kondo, S. (1999). Zebrafish leopard
gene as a component of the putative reaction-diffusion system. Mech Dev 89,
87-92.

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Nakamasu, A., Takahashi, G., Kanbe, A. & Kondo, S. (2009). Interactions
between zebrafish pigment cells responsible for the generation of Turing
patterns. Proc Natl Acad Sci U S A 106, 8429-34.

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Yamaguchi, M., Yoshimoto, E. & Kondo, S. (2007). Pattern regulation in the
stripe of zebrafish suggests an underlying dynamic and autonomous mechanism.

Proc Natl Acad Sci U S A 104, 4790-3.
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Watanabe, M., Watanabe, D. & Kondo, S. (2012). Polyamine sensitivity of gap
junctions is required for skin pattern formation in zebrafish. Sci Rep 2, 473.

17.

Watanabe, M. & Kondo, S. (2012). Changing clothes easily: connexin41.8
regulates skin pattern variation. Pigment Cell Melanoma Res 25, 326-30.

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࿑࿑ 1                  ࿑㧞࿑㧟                  ࿑㧠࿑㧡                   ࿑㧢࿑㧣᬴ܱဇǷȭȍǺȟƷឪเʮᣃ‫ٻ‬ȷҔȷѣཋ᬴ܱ଀ᚨ
ȊǷȧȊȫȐǤǪȪǽȸǹȗȭǸǧǯȈžȩȃȈſ
ࡉஜ᭗᬴࣓ܱဇǷȭȍǺȟƸŴɭမɶưƻǖƘဇƍǒǕƯƍǔˊᘙႎƳ᬴ܱѣཋưƢŵƠƔƠŴƦƷឪเƸჷǒǕƯƍǇƤǜưƠƨŵ঻ŷƸŴμɭမưМဇƞǕƯƍǔǷȭȍǺȟ 117 ኒወƷ DNA ǛᛦǂƯŴƢǂƯƷኒወƕσᡫƠƯƨƬƨƻƱƭƷǢȫȓȎᆳ໱‫ီ٭‬ǛਤƬƯƍǔƜƱǛᆳƖഥǊǇƠƨŵƞ
ǒƴŴƜƷǢȫȓȎ‫ီ٭‬ƸŴǇƩǒ೉ಮǛǋƬƨȩȃȈƴဃơƨӧᏡࣱƕ᩼ ࠝ ƴ ᭗ ƍ Ɯ Ʊƕ ǘƔ Ǔ Ǉ Ơ ƨ ŵ ȩ ȃ Ȉ ƕ ܱ ᬴ ѣ ཋ ҄ ƞ Ǖ ƨ 1850 ࠰ Ɲ ǖƷȨȸȭȃȑưƸŴǷȭȍǺȟǋŴǇƩǒȍǺȟǋ᬴ܱƴ̅ǘǕƯƍǇƠ
ƨŵɲᎍƕσƴᚡƞǕƯƍǔஇӞƷ૨ྂƸŴ঻ŷƷᛦǂƨᨂǓŴ‫קٻ‬ưЈ༿ ƞ Ǖ ƨ ƀ ᫱ ᱝ ྚ Ʒ Ɣ ƚ Ƹ Ơ Ტ 1775 Უ Ɓ ư Ƣ ŵ Ɯ Ɯ ư Ƹ Ŵ ß Ǉ Ʃ ǒ à ǍßႸហƷႉàƱƍƬƨྦྷƠƍȍǺȟƕग़ྜྷѣཋƱƠƯኰʼƞǕƯƍǇƢŵ
ǋƠƔƠƨǒŴଐஜưग़ྜྷѣཋƱƠƯ᫫ᏋƞǕƯƍƨȍǺȟƕ൶ৎ଺ˊƴȨȸȭȃȑƴบǓŴƦƷࢸ቟‫׎‬ǛኺƯŴྵ‫נ‬ƷǑƏƴɭမƴ࠼ǇƬƨƷƔ
ǋƠǕǇƤǜŵ

ǷȭȍǺȟƱƸŴǢȫȓȎȩȃȈƷƜƱŵ
ỈẵỚίᱝὸểẟạԧᛖỊẆẒԧ๮ɤৗ‫˟׋‬ẓί1712 ࠰ὸỆợỦểẆἻἕἚί‫ܖ‬Ӹ Rattus norvegicusẆԧӸἛἨ἟ἌἱὸỉẮểửẰẴẇẼễỚỆẆἰỸἋ ί ԧ Ӹἡ἖ ỽ἟ Ἄ ἱὸỊ Ẇ ỉ Ụ Ắ ί [ ᱝ ӟ][ ᱝ ᩷] ὸ ểԠị ủ ề ẟ Ẻ(Kuramoto 2011)ẇ
ࢼẾềẆἉἿ἟ἌἱểỊẆႉẟἻἕἚẇẴễỪẼẆൗᑥỉႉẟἻἕἚỉẮểỂẝ
ỦẇൗᑥỉႉẟἻἕἚỉɶỆờẆაầហẟờỉể᱅ẟờỉầẝỦẇႸហỉႉểỊẆྵ‫נ‬ỂẟạẆỴἽἥἠỂẝỦẇộẺẆႸ᱅ỉႉểỊẆྵ‫נ‬ỂỊẆKit ᢡˡ‫ࡈ܇‬ỉ‫˳ီ٭‬ỆᙸỤủỦẇȩȃȈǛဇƍƨᄂᆮƷഭӪἻἕἚỊ 1850 ࠰ắỨẦỤ‫ܖ‬ᘐᄂᆮỆဇẟỤủẺẇἻἕἚửМဇẲẺờẾể
ờӞẟ‫ܖ‬ᘐᛯ૨ỊẆ௿᫱‫ܖ‬Ệ᧙ẴỦờỉỂẆᒍ‫׎‬ʴ Savory ỆợẾề᬴ܱဇἉἿ἟Ἄἱẇέ‫ټ‬ႎỆἳἻἝὅᑥእầӳ঺ỂẨễẟẺỜ
ỆẆႉẟൗᑥẆហẟაểễỦẇ

1863 ࠰Ệ Lancet ᛏỆπᘙẰủềẟỦ(Savory 1863)ẇ1885 ࠰ỆỊẆἛỶ἖ʴ Crampe ầẆἻἕἚửဇẟẺʩᣐ᬴ܱỂẆἳὅἙἽỉẒᢡˡỉඥЩẓầՌʐѣཋỂờ঺ụᇌếẮểửᅆẲềẟỦ(Crampe 1885)ẇ

ࢬỤỊẆGrey ἻἕἚί᣼ဃᑥὸẆAlbino ἻἕἚίỴἽἥἠὸẆPiebald ἻἕἚίộẻỤẆẝỦẟỊẆ᪽ࠉ૮ὸỉ 3 ᆔ᫏ỉἻἕἚửဇẟề᬴ܱẲẺẇếộụẆ
1850 ࠰ắỨỆỊẆ᣼ဃᑥἻἕἚỆьảẆỴἽἥἠἻἕἚểộẻỤἻἕἚầ‫܍‬‫נ‬ẲềẟẺẇ

Wistar ᄂᆮ৑Ʊ Donaldson᬴ܱဇộẻỤ἟Ἄἱẇ
Hooded ‫ီ٭‬ửἭἴỆờếẮểỂẆ˳ൗỉᑥእЎࠋầ‫٭‬ỪụẆᏟᢿẦỤᐞᢿầႉẪễỦẇ᪽ᢿẦỤɥᏹᢿỆỉỚᑥእầЎࠋẲẆẝẺẦờẐ᪽ࠉẑửẦốẾẺẦỉợạễ೉ಮỆễỦẇẸỉẺỜẮỉợạ
ễ೉ಮửẐ᪽ࠉ૮ẑểԠốẇ

1900 ࠰ˌᨀỉẆἻἕἚኒወỉᄩᇌᢅᆉỉഭӪỊẆẦễụଢᄩễᚡ᥵ểẲềസẾềẟỦẇễẦỂờẆ‫ٻ‬ẨễࢫлửௐẺẲẺỉỊẆWistar ᄂᆮ৑ỂẝỦẇ1906 ࠰ Donaldson ầᇹ 3 ˊỉ৑ᧈểễụẆỴἽἥἠἻἕἚỉ೅แ҄ỆӕụኵỮẻẇẸỉࢸẆWistar ἻἕἚỊẆỴἽἥἠἻἕἚỉˊᘙ఍ểẲềྵ‫נ‬Ểờ࠼ẪဇẟỤủềẟỦẇɭမỂМဇẰủềẟỦἻἕἚỉኖҞૠỊỸỵἋἑὊᄂᆮ৑ဌஹểẰủỦẇ
ỂỊẆWistar ᄂᆮ৑ỆਤẼᡂộủẺἻἕἚỊỄỉợạễờỉễỉỂẝẾẺ
ỉẻỨạẦᾎ
Donaldson ầᡓỔềẟỦ(Donaldson 1915)ẇḛἻἕἚỊẆ᣼ဃộẺỊ᫫ẟᬘỤẰủẺờỉửλ৖ỂẨẺẇࢸᎍỊẆỴἽἥἠẦẆộẻỤầɼỂẝẾẺẇ
ỴἽἥἠỉဌஹỊẆọểếễỉẦᙐૠễỉẦỪẦỤễẦẾẺẇἺὊἿἕἣ
ỉἅἿἝὊỆ᧙̞ẲềẟỦỉẦờỪẦỤễẦẾẺẇḜW ISTAR ᄂᆮ৑
1894 ࠰ẆἬὅἉἽἫἝỴ‫ٻ‬ὉҔὉᚐа‫ܖ‬૙ܴ C ASPERW ISTAR ૙੉ửᚡࣞẲềᚨᇌẇ1905 ࠰Ẇᅕኺ‫ܖ‬Ẇൔ᠋ᚐа‫ܖ‬Ẇႆဃ‫ܖ‬Ệ໰ໜẇ
1906 ࠰ẆD ONALDSON ầᄂᆮ৑ᧈểễụẆỴἽἥἠἻἕἚỉ೅แ҄ỆӕụኵớẇɭမỂМဇẰủềẟỦἻἕἚỉኖҞૠỊỸỵἋἑὊᄂᆮ৑ဌ
ஹểẰủỦẇ

ếộụẆWistar ᄂᆮ৑ỆਤẼᡂộủẺἻἕἚỉ‫ٶ‬ẪỊẆỴἽἥἠἻἕἚểộẻỤἻἕἚỂẝẾẺầẆỴἽἥἠἻἕἚỉឪเỊἺὊἿἕἣỉᄂᆮܴỂ̅ỪủềẟẺỴἽἥἠἻἕἚỂẝẾẺỉẦẆẝỦẟỊẆ቟‫׎‬Ể਺ྒẰủẺ᣼ဃἻἕἚỉỴἽἥἠ‫˳ီ٭‬ỂẝẾẺỉẦỊɧଢỂẝẾẺợạẻẇ

Ў‫܇‬ᢡˡ‫ܖ‬ႎƳǢȗȭȸȁưǷȭȍǺȟƷឪเǛ੕ǔྵ‫נ‬МဇỂẨỦỴἽἥἠἻἕἚởộẻỤἻἕἚỉ‫ٻ‬Ҟầ Wistar ᄂᆮ৑ဌஹỂẝỦẇẸẮỂẆỴἽἥἠ‫٭‬ီể hooded ‫ီ٭‬ίộẻỤ೉ಮỉҾ‫ီ٭׆‬ὸỉᢡˡ‫܇‬ἾἫἽỂỉ‫ီ٭‬ửཎ‫ܭ‬ẲẆẸủỤỉ‫ီ٭‬ỉஊ໯ửྵ‫נ܍נ‬ẲềẟỦἻἕἚầਤẾềẟỦẦỄạẦửᛦỔỦẮểỆẲẺẇ

ỴἽἥἠ‫ီ٭‬ỆếẟềỊἓἿἉἜὊἎᢡˡ‫܇‬ỉἱἋἍὅἋ‫ီ٭‬ίArg299HisὸỂẝỦẮểầẴỂỆ‫إ‬ԓẰủềẟẺ(Blaszczyk et al. 2005)ẇἜἉἹἜἽἢỶỼἼἏὊἋἩἿἊỹἁἚẐἻἕἚẑửᡫẳềɭမӲ‫׎‬ẦỤᨼỜỤủẺ 117 ኒወỉỴἽἥἠἻἕἚኒወử‫ݣ‬ᝋỆẆArg299His ‫ီ٭‬ỉஊ໯ửᛦỔẺẇẸỉኽௐẆẴỔềỉኒወầӷɟỉỴἽἥἠ‫ီ٭‬ửờẾềẟỦẮểầЎẦẾẺẇếộụẆɭမɶỂờẼẟỤủềẟỦ
ỴἽἥἠἻἕἚኒወỆỊẆឪเểễỦỴἽἥἠἻἕἚầẟẺẮểầЎẦẾẺẇɟ૾ỉ hooded ‫ီ٭‬ỆếẟềỊẆᇹ 14 ௨ᑥ˳ỉ D14Rat84 ể D14Got40 ỉ᧓ỆἰἕἦὅἂẰủềấụẆ
ͅᙀᢡˡ‫܇‬ểẲề Kit ᢡˡ‫܇‬ầਫậỤủềẟẺ(Torigoe et al. 2011)ẇ
ẸẮỂẆhooded ᢡˡ‫܇‬ỉӷ‫ܭ‬ửᚾỚẺẇộẵẆ897 ᪽ỉ৏Ẳʩᩃ‫܇‬ểἻἕἚᡈʩኒỉ SNP ἰὊỽὊỆợỦἡἩἿἑỶἩᚐௌửᘍẟẆhooded ࡈˮử Kit ᢡˡ‫܇‬ỉɥ්ửԃớኖ 92kb ỉἄἠἲ᪸؏Ệ዁ụᡂỮẻẇഏẟỂẆhooded ‫ီ٭‬ửᙸếẬỦẺỜỆẆhooded ࡈˮửỽἢὊẴỦ BAC ἁἿὊὅửҥᩉẲẆẸỉỶὅἇὊἚᣐЗửൿ‫ܭ‬ẲẺẇ‫ݣ‬ᝋểễỦ BAC ἁἿὊὅỊẆhooded ‫ီ٭‬ἭἴỂẝỦ F344/Stm ἻἕἚể
LE/SῃᾼἻἕἚẦỤ˺ᙌẲẺờỉỂẝỦẇF344 ဌஹỉ BAC ἁἿὊὅ RNB1-043O06 ể LE/Stm ဌஹỉ
BAC ἁἿὊὅ RNB1-272P10 ầ hooded ࡈˮửԃỮỂẟỦẮểửᄩᛐẲẆỶὅἇὊἚỉᣐЗửൿ‫ܭ‬ẲẺẇẸỉኽௐẆɲኒወỂỊẆKit ᢡˡ‫܇‬ỶὅἚἿὅ 1 ϋỆ 5,930bp ỉϋ‫ࣱנ‬ἾἚἿỸỶἽἋᣐЗầ਴λẰủềẟỦẮểầЙଢẲẺẇἜἉἹἜἽἢỶỼἼἏὊἋἩἿἊỹἁἚẐἻἕἚẑửᡫẳềᨼỜỤủẺ 55 ኒወỉஊᑥἻἕἚኒወử‫ݣ‬ᝋỆẆẮỉ਴λ‫ီ٭‬ỉஊ໯ửᛦỔẺẇộẻỤἻἕἚ 37 ኒወμềầẆ਴λ‫ီ٭‬ửਤẾềẟẺẇɟ૾Ẇ᣼ဃ‫׹‬ൗᑥỉኒወ 18 ኒወỊẆ਴λ‫ီ٭‬ửờẾềẟễẦẾẺẇẴễỪẼẆ਴λ‫ီ٭‬ỉஊ໯ểẆộẻỤ೉ಮỉஊ໯ầ‫ܦ‬μỆɟᐲẲẺẇˌɥỉẮểẦỤẆộẻỤ೉ಮỉҾ‫׆‬ίhooded ‫ီ٭‬ὸỉദ˳ỊẆKit ᢡˡ‫܇‬ϋỉ਴λ‫ီ٭‬ỂẝỦểኽᛯẲẺẇ䍟䍹䍚䍣䍎䍜䍼ᄌ⇣

Kit
Kit ᄌ⇣䈅䉍䋼䉁䈣䉌䋾

KRRGHG(59
LTR

5,930 bp䈭䈚
LTR

Kit ᢡˡ‫܇‬ϋỉ਴λ‫ီ٭‬
Hooded ᢡˡ‫܇‬ửἭἴỆਤếἻἕἚỂỊẆKit ᢡˡ‫܇‬ỶὅἚ
Ἷὅ 1 Ệϋ‫ࣱנ‬ἾἚἿỸỶἽἋỉ਴λ‫ီ٭‬ầỚỤủẺẇ

䈅䉍䋼䉝䊦䊎䊉䋾

117

䈭䈚
䋼᦭⦡䋾

37
0

18

ᾀᾆᾁἻἕἚᡈʩኒỉᢡˡ‫׹܇‬
172 ኒወỉᢡˡ‫׹܇‬ử Kit ਴λ‫ီ٭‬ỉஊ໯ểỴἽἥἠ‫ီ٭‬ỉஊ໯ỂЎ᫏ẲẺẇஊᑥኒወỂỊẆ਴λ‫ီ٭‬ỉஊ໯ểẆộẻỤ೉ಮỉஊ໯ầ‫ܦ‬μỆɟᐲẲẺẇɟ૾ẆỴἽἥἠኒወỂỊẆᛦỔẺμềỉኒወầ
Kit ਴λ‫ီ٭‬ửਤẾềẟẺẇ

μƯƷǢȫȓȎȩȃȈኒወƸŴhooded ‫ီ٭‬Ǜǋƭ
Hooded ‫ီ٭‬ỉദ˳ầЙଢẲẺẮểỂẆỴἽἥἠἻἕἚኒወầ hooded ‫ီ٭‬ửਤếẦỄạẦửᢡˡ‫܇‬ᚮૺỂẨỦợạỆễẾẺίỴἽἥἠἻἕἚỂỊẆᑥእẸỉờỉầ˺ụЈẰủễẟẇẸỉẺỜẆhooded ‫ီ٭‬ửਤẾềẟềờẆẸỉјௐẆẴễỪẼẆộẻỤ೉ಮầ‫ٳ‬ỆྵỪủễẟὸẇẸẮỂẆỴἽἥἠἻἕἚኒወ 117ኒወửᛦỔẺẇẸỉኽௐẆμềỉỴἽἥἠἻἕἚኒወầẆKit ᢡˡ‫܇‬ỉ਴λ‫ီ٭‬ửờẾềẟỦẮểầЎ
ẦẾẺẇ(Kuramoto et al. 2012)ˌɥỉኽௐẦỤẆˌɦỉ 2 ໜầᎋảỤủẺẇ
ṞẐἉἿ἟ἌἱẑỉឪเểễỦɟ᪽ỉ἟ἌἱίỴἒἲẝỦẟỊỶἨὸầẟẺẇṟẸỉẐἉἿ἟ἌἱẑỊẆẐộẻỤ἟ἌἱẑẦỤЈྵẲẺẇ䉁䈣䉌䊈䉵䊚

+RRGHG⮘䟿ẐἉἿ἟ἌἱẑểẐộẻỤ἟Ἄἱẑểỉ᧙̞ɭမɶỉẐἉἿ἟ἌἱẑỊ̊‫ٳ‬ễẪẆKit
ᢡˡ‫܇‬ỉ‫ီ٭‬ửờếẇẮỉẮểỊẆẐộ
ẻỤ἟ἌἱẑỆỴἽἥἠ‫ီ٭‬ầဃẳẺểᎋảủịẆᛟଢầếẨởẴẟẇ

Hoodedᄌ⇣䋺䈅䉍䍏䍷䍩䍼䍧ᄌ⇣䋺䈭䈚ቿወኰካ⮘䟿
㊁↢䊈䉵䊚

䉁䈣䉌䊈䉵䊚

Hoodedᄌ⇣䋺䈭䈚䍏䍷䍩䍼䍧ᄌ⇣䋺䈭䈚

Hoodedᄌ⇣䋺䈅䉍䍏䍷䍩䍼䍧ᄌ⇣䋺䈭䈚䉲䊨䊈䉵䊚
Hoodedᄌ⇣䋺䈅䉍䍏䍷䍩䍼䍧ᄌ⇣䋺䈅䉍ૼƨƳᜋᲴžǇƩǒȍǺȟſƱžǷȭȍǺȟſƷឪเƸᲹẸủỂỊẆ1850 ࠰ˌЭỉἻἕἚỆ᧙ẴỦᚡ᥵ỆỊẆộẻỤ἟ἌἱểἉἿ἟ἌἱỆ᧙ẴỦᚡ᠍ỊẝỦỉỂ
ẝỨạẦẇ
ộẵẆ19 ɭኔЭҞỆʴൢỉẝẾẺۗಏỂẝỦ Rat Baiting ầẝỦẇẮủ
ỊἀἵὅἨἽỉɟᆔỂẆἘἼỴཚễỄỉོཚầẆἦἕἚỆ્ẺủẺɟ‫ܭ‬ૠί̊ảị 100 ᪽ὸỉἻἕἚửμềẦỚൈẴộỂỉ଺᧓ửឌẬỉ‫ݣ‬ᝋểẴỦẇ࢘໱ỉẮểễầỤ‫ٶ‬ૠỉἻἕἚầ࣏ᙲểễỦẇἻἕἚử਺ྒẝỦẟỊጛ഻ẲẺợạỂẝỦầẆẸỉᢅᆉỂỴἽἥἠἻἕἚầႆᙸẰủẺểᎋảỤủềẟỦ(Richter 1954)ẇẲẦẲẆẮỉᛅỊ঻ẉỉኽௐểႻẟλủễẟẇ঻ẉỉኽௐẦỤẆỴἽἥἠἻἕἚỊộẻỤἻἕἚẦỤЈྵẲẺểễẾềẟỦ
ẦỤỂẝỦẇ

Rat baiting ẦỤҞɭኔỖỄЭỉ 1787 ࠰ỆỊẆଐஜỉ‫קٻ‬ỂẒྦྷᎃᱝᏋᒬẓầЈ༿ẰủềẟỦẇẮỉ
ஜỊẆྦྷᎃᱝếộụẆྦྷẲẟग़ྜྷဇ἟Ἄἱỉዒˊ૾ඥửᚡẲẺờỉỂẝỦẇẮẮỆẆḛốẼίộẻỤὸḜẝỦẟỊḛႸហỉႉḜỉ἟ἌἱầኰʼẰủềẟỦẇ
ẰỤỆẆẒྦྷᎃᱝᏋᒬẓợụ 12 ࠰Эỉ 1775 ࠰ỆẆẒ᫱ᱝྚỉẦẬỊẲẓ
ầ‫קٻ‬ỂЈ༿ẰủềẟỦẇẮỉஜỊẆ἟Ἄἱỉ᫫Ꮛ୿ỉᇹɟӭỂẝụẆ˺ᎍỉବࠌ‫ؘ‬ɼʴỊẆ἟Ἄἱỉग़‫ܼڤ‬ỂྦྷẲẟ἟Ἄἱử‫ٶ‬ૠ᫫ᏋẲềẟẺẇẮỉɶỆẆộẻỤ἟ἌἱểἉἿ἟ἌἱỆ᧙ẴỦᚡ᠍ầẝỦẇḛ૮ᱝίộẺỤỈẴỚὸỊႉ᱅ỉộẻỤễụẇଢԧ࠰ɶẇ‫קٻ‬ি᫱ᱝܼίợạẸẦὸ̖ửᝲẲềẇ‫ڈ‬ԼửྜྷίờềẝẸịὸủẲỆẇИềငẴẇḜḛ ႉᱝỊ࠰ửഭề‫٭‬ặẲờỉỆỊẝỤẵẇКỆɟᆔỉờỉỆềẇ

Ẓ᫱ᱝྚỉẦẬỊẲẓỉ਴ዋίᢿЎਘ‫ٻ‬ὸ἟Ἄἱ‫ދ‬ỉɼʴầ৖ỉɥỆộẻỤ἟ἌἱửỉẶềẟỦẇ

υɥԼểẴỦờỉỊẆ‫ᧈރ‬ẪൗạỦỊẲẪẇ᎚‫ٻ‬Ệ᫊ᧈẪაហẲẇḜẮỉợạỆẆଐஜỂỊ൶ৎ଺ˊẆ἟Ἄἱử᫫ẟᬘỤẲềἬἕἚểẲề᫫ạểẟạᝅẦễ૨҄ầẝẾẺẇ
൶ৎ଺ˊẆଐஜỊᧈ߃ỉЈ޽ửᡫẳềἺὊἿἕἣểếễầẾềẟẺẇọỢẾểẲẺỤẆợẪ᫫ẟᬘỤẰủẺྦྷẲẟൗᑥỉ἟ἌἱửἺὊἿἕἣʴẺẼầᅑ‫׎‬ỆਤẼ࠙ụẆẸủỤầग़ྜྷѣཋểẲề‫ٻ‬ЏỆ᫫ᏋẰủẺỉẦờẲủễẟẇẸẲềẆẸỉ‫ܘ܇‬ầ 1850 ࠰ắỨẆৢẟởẴẟểẟạྸဌỂẆ᬴ܱဇỉѣཋỆМဇẰủẺỉỂỊễẟẦẇἉἿ἟ἌἱởộẻỤ἟Ἄἱỉឪเử੕ẾềẟẪểẆక‫ٳ‬ẆଐஜẸủờ᧙ᙱẆểẪỆ‫ٻ‬‫ק‬ỆᘍẨ࢘ẺỦẦờẲủễẟẇ
൶ৎ଺ˊỉɥ૾ဌஹỉἉἿ἟ἌἱởộẻỤ἟ἌἱầẆྵ‫נ‬ỉ᬴ܱဇἻἕἚỉᅑέểễụẆ21 ɭኔỉᄂᆮửૅảềẟỦẇẮỉợạỆ࣬ẟửỜẫỤẴẮểầỂẨỦẇ

1890ᐕ䈗䉐

ᳯᚭᤨઍ䋿

1945ᐕ䈗䉐

1700 ࠰ࢸҞẆଐஜỉɥ૾ỂỊẆ἟Ἄἱửग़ྜྷѣཋểẲề᫫ạ૨҄ầẝẾẺẇྦྷẲẟൗᑥỉ἟ἌἱẺẼỊẆ഑቟ỆบẾẺӧᏡࣱầẝỦẇẸỉࢸẆଐஜဌஹỉἻἕἚẺẼỊἺὊἿἕἣỂ᫫ᏋẰủ Ẇ 1850 ࠰ ắ Ứ ܱ ᬴ Ệ ဇẟỤủẺẦờẲủễẟẇ
1890 ࠰ắỨỆỊ቟‫׎‬ிᢿỆ‫ݰ‬λẰủẆஜ఍ႎỆ᬴ܱѣཋểẲềМဇẰủẺẇ
Wistar ᄂᆮ৑ửᡫẲềẆμɭမỆ࠼ộẾẺẇ
ẮỉợạỆ࣬ẟửỜẫỤẴẮ
ểờỂẨợạẇ

ᜓᠴஜᄂᆮỊἜἉἹἜἽἢỶỼἼἏὊἋἩἿἊỹἁἚẐἻἕἚẑỉɟ࿢ểẲềᘍỪủẺẇἜἉἹἜἽἢỶỼἼἏὊἋἩἿἊỹἁἚẐἻἕἚẑỉನ঺ՃẆཎỆẆᑹ߷ࣙ‫پ‬૙੉ẆBirger Voigt ᄂᆮՃẆɶᙱᎧ২ᘐ‫ᧉݦ‬ᎰՃỆࣂᅇဎẲɥậộẴẇӋᎋ૨ྂ
Blaszczyk WM, Arning L, Hoffmann KP, Epplen JT (2005) A Tyrosinase missense mutation causes
albinism in the Wistar rat. Pigment Cell Res 18, 144-145
Crampe H (1885) Landwirthschaftliche Jahrbücher.
Donaldson HH (1915) The Rat data and reference tables.
Kuramoto T (2011) Yoso-tama-no-kakehashi; the first Japanese guidebook on raising rats. Exp Anim
60, 1-6
Kuramoto T, Nakanishi S, Ochiai M, Nakagama H, Voigt B, Serikawa T (2012) Origins of albino andhooded rats: implications from molecular genetic analysis across modern laboratory rat strains. PLoS
One 7, e43059
Richter CP (1954) The effects of domestication and selection on the behavior of the Norway rat. J NatlCancer Inst 15, 727-738
Savory WS (1863) Experiments on food; its destination and uses. Lancet 1, 381-383
Torigoe D, Ichii O, Dang R, Ohnaka T, Okano S, Sasaki N, Kon Y, Agui T (2011) High-resolution
linkage mapping of the rat hooded locus. J Vet Med Sci 73, 707-710

⢽⾰ઍ⻢࡮േ⣂⎬ൻ⎇ⓥߦ߅ߌࠆ࠙ࠨࠡߩ᦭↪ᕈ- ࡅ࠻ apoA-II ࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ࠙ࠨࠡߩ㐿⊒ߣᔕ↪
ዊᳰᥓ਽ 1‫ޔ‬ർ᎑ୃม 2‫⷏ޔ‬ፉ๺ବ 2‫ޔ‬ᷰㄝᾖ↵ 2‫ޔ‬Ⴎ⷗㓷ᔒ 1‫▸ޔ‬ᳯᨋ 3
1 ␹ᚭᄢቇᄢቇ㒮කቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳
2 ૒⾐ᄢቇ✚วಽᨆታ㛎࠮ࡦ࠲࡯↢‛⾗Ḯ㐿⊒ㇱ㐷
3 ጊ᪸ᄢቇᄢቇ㒮කቇᎿቇ✚ว⎇ⓥㇱಽሶ∛ℂቇ⻠ᐳߪߓ߼ߦታ㛎േ‛ߣߒߡߩ࠙ࠨࠡߪ‫ޔ‬⢽⾰ઍ⻢߇ࡅ࠻ߦ㘃ૃߒࠦ࡟ࠬ࠹ࡠ࡯࡞㘩ߩᛩਈߦࠃ
ࠅേ⣂⎬ൻࠍኈᤃߦ⊒∝ߔࠆߎߣ߆ࠄ‫ޔ‬⢽⾰ઍ⻢࡮േ⣂⎬ൻߩಽ㊁ߢฎߊ߆ࠄᵴ↪ߐࠇߡ߈ߚ‫ߩߎޕ‬೑ὐࠍ↢߆ߒ‫ޔ‬⢽⾰ઍ⻢ߥࠄ߮ߦേ⣂⎬ൻߦ㑐ࠊࠆㆮવሶߩᯏ⢻⸃ᨆ㧔࿃ᨐ㑐ଥߩᬌ⸛‫ޔ‬ಽሶᯏᐨߩ⸃᣿╬㧕ߦ࠙ࠨࠡࠍᔕ↪ߔߴߊ‫ޔ‬ᒰ⎇ⓥࠣ࡞࡯ࡊߪㆮ
વሶᡷᄌ࠙ࠨࠡߩ㐿⊒ߣ⎇ⓥᔕ↪ߦᧄ㇌ߢೋ߼ߡ⌕ᚻߒ‫␹ޔߦࠄߐޔ‬ᚭᄢቇߦߡ᮸┙ߐࠇߚ WHHL ࠙ࠨࠡࠍᵴ↪ߔࠆߎߣߢ‫ޔ‬⢽⾰ઍ⻢ߥࠄ߮ߦേ⣂⎬ൻߩ key moleculeߩᯏ⢻⸃ᨆࠍ⁛⥄ߦផㅴߒߡ߈ߚ(1)‫ޕ‬ᧄⓂߢߪ‫ޔ‬⢽⾰ઍ⻢࡮േ⣂⎬ൻ⎇ⓥߦ࠙ࠨࠡߩᨐߚߒߡ߈ߚᓎഀࠍ᭎⺑ߒ‫ޔ‬ᒰࠣ࡞࡯ࡊߢផㅴߒߡ޿ࠆ࠙ࠨࠡߩㆮવሶᡷᄌࡕ࠺࡞ߦࠃࠆᚑᨐࠍ଀␜ߔࠆߎߣߢ‫ࠡࠨ࠙ޔ‬ࡕ࠺࡞ߩ᦭↪ᕈߩ৻┵ࠍ߅␜ߒߒߚ޿‫ޕ‬࠙ࠨࠡߣ⢽⾰ઍ⻢࡮േ⣂⎬ൻ⎇ⓥߩᱧผ േ⣂⎬ൻ߇⊒↢ߔࠆⷐ࿃ࠍᩕ㙃ቇߩⷰὐ߆ࠄ⸃᣿ߒࠃ߁ߣ޿߁⹜ߺ߇ 1900 ᐕઍߩ೨ඨ߆ࠄⴕࠊࠇߚ‫ޕ‬Ignatowski ߪࡅ࠻ߢᓧࠄࠇߚ⍮⷗ߦၮߠ߈‫ߦࠡࠨ࠙ޔ‬⡺ࠍਈ߃േ⣂⎬ൻࠍ⊒↢ߐߖࠆߎߣߦᚑഞߒߚ(2)‫ߚߒ↪૶ࠍࠡࠨ࠙ߩߎޕ‬ታ㛎♽ߪએ㒠ߩᄙߊߩ⎇ⓥߢណ↪ߐࠇ‫ޔ‬േ⣂⎬ൻࠍ⿠ߎߔ㘩᧚㧔Ⱞ⊕⾰࡮⢽⾰㧕߇⛉ࠅㄟ߹ࠇߡ޿ߞߚ(3)‫ޕ‬ߘߒߡ‫ ߤ߁ࠂߜࠄ߆੹ޔ‬100 ᐕ೨‫ߩࠕࠪࡠޔ‬ታ㛎∛ℂቇ⠪ Anitschkow ࠄߦࠃࠅ‫ࠦޔ‬࡟ࠬ࠹ࡠ࡯࡞㧔ᬀ‛ᴤߦᷙว㧕ߩᛩਈߢ࠙ࠨࠡߦേ⣂⎬ൻࠍᗖ⿠ߢ߈ࠆߎߣ߇ⷰኤߐ
ࠇ‫⃻ޔ‬࿷ቯ⺑ߣߥߞߚ‫́࡞࡯ࡠ࠹ࠬ࡟ࠦޟ‬േ⣂⎬ൻ‫ޠ‬઒⺑ߩ⏕┙ߦᄢ߈ߊ⽸₂ߒߚ(4)‫ޕ‬ߘߩᓟ߽⢽⾰ઍ⻢࡮േ⣂⎬ൻ⎇ⓥߩᭂ߼ߡ㊀ⷐߥ⊒⷗ߦ࠙ࠨࠡ߇⽸₂ߒߡ޿ࠆ‫․ޕ‬
ߦ 1973 ᐕߦߪ‫ޔ‬ᄢ߈ߥ 3 ߟߩ⊒⷗߇޽ߞߚ‫ޕ‬1 ߟߪ‫ޔ‬WHHL ࠙ࠨࠡߩ⒅ߣߥࠆ㜞⢽ⴊ∝࠙ࠨࠡ߇␹ᚭᄢቇേ‛ታ㛎ᣉ⸳ߩᷰㄝߦࠃߞߡ⊒⷗ߐࠇߚ(5)‫ޕ‬2 ߟ߼ߪ‫⚦ޔ‬⢩ߩࠦ࡟ࠬ࠹ࡠ࡯࡞ઍ⻢ߦฃኈ૕ࠍ੺ߔࠆ♽߇ሽ࿷ߔࠆน⢻ᕈ㨧ૐᲧ㊀࡝ࡐⰮ⊕(LDL)ฃ
ኈ૕઒⺑㨩߇‫☨ޔ‬࿖࠹ࠠࠨࠬᄢቇߩ Goldstein, Brown ࠄߦࠃߞߡឭ໒ߐࠇߚ(6)‫ޕ‬ᓐࠄߪߘߩᓟ LDL ฃኈ૕ߩ⊒⷗ߦࠃߞߡ઒⺑ࠍታ⸽ߒ‫⚦ޔ‬⢩ౝࠦ࡟ࠬ࠹ࡠ࡯࡞ઍ⻢⺞▵♽ߩోኈࠍ⸃᣿ߒߚഞ❣ߦࠃߞߡ 1985 ᐕߦࡁ࡯ࡌ࡞⾨ࠍฃ⾨ߒߚ߇‫⎇ߩߘޔ‬ⓥߦߪ
ᓐࠄߦಽਈߐࠇߚ WHHL ࠙ࠨࠡ߇ᄢ߈ߊ⽸₂ߒߡ޿ߚ‫ޕ‬WHHL ࠙ࠨࠡߩᄌ⇣ㆮવሶ
߇ LDL ฃኈ૕ߢ޽ߞߚߣ޿߁஧ὼ߇‫⎇ޔ‬ⓥߩቢᚑࠍᩰᲑߦᣧ߼ߚ‫ޕ‬3 ߟ߼ߪ‫ޔ‬㜞ࠦ࡟ࠬ࠹ࡠ࡯࡞ⴊ∝ߩᴦ≮⮎ߣߒߡ⃻࿷਎⇇ߢᦨ߽ᄙߊಣᣇߐࠇࠆࠬ࠲࠴ࡦ♽⮎೷
㧔HMG-CoA ㆶర㉂⚛㒖ኂ೷㧕߇‫ޔ‬ਃ౒⵾⮎ߩ㆙⮮ࠄߦࠃߞߡ⊒⷗ߐࠇߚ‫ޕ‬േ‛ࠍ↪
޿ߚ೨⥃ᐥ⹜㛎ߢߪߍߞᱤ㘃ߦᛩਈߒߡ߽ⴊਛࠦ࡟ࠬ࠹ࡠ࡯࡞ߩૐਅߪ⹺߼ࠄࠇߥ߆
ߞߚ߇‫ޔ‬ਛ~ᄢဳേ‛ߩࠗ࠿‫ߦࠡࠨ࠙ޔ࡝࠻ࡢ࠾ޔ࡞ࠨޔ‬ᛩਈߒߚߣߎࠈࠦ࡟ࠬ࠹ࡠ࡯
࡞߇⪺᣿ߦૐਅߒ‫ߩߘޔ‬ലᨐߪ࠙ࠨࠡߢᦨ߽㗼⪺ߦ⷗ࠄࠇߚ(7)‫ޕ‬⢽⾰ઍ⻢࡮േ⣂⎬ൻ⎇ⓥߦ߅ߌࠆ࠙ࠨࠡߩ೑ὐ ⢽⾰ઍ⻢࡮േ⣂⎬ൻߩಽ㊁ߢ࠙ࠨࠡ߇⽸₂ߢ߈ߚ⢛᥊ߦߪ‫ߩߘޔ‬⢽⾰ઍ⻢߇ઁߩߍߞᱤ㘃ࡕ࠺࡞ߣᲧߴ‫ߦ࠻ࡅޔ‬ㄭ޿ߎߣ߇ᒝߊ㑐ଥߒߡ޿ࠆ‫ ⴫ޕ‬1 ߦ␜ߔࠃ߁ߦ‫ࠨ࠙ޔ‬ࠡߩⴊਛߦߪ࡝ࡐⰮ⊕ઍ⻢ߦ㊀ⷐߥࠦ࡟ࠬ࠹࡝࡞ࠛࠬ࠹࡞ォㅍⰮ⊕(CETP)߇޽ࠆ‫ߎޕ‬ߩⰮ⊕ߪ‫▤ⴊࠍ࡞࡯ࡠ࠹ࠬ࡟ࠦޔ‬ოߦㆇ߱̌ᖡ₹̍࡝ࡐⰮ⊕ߩ LDL ߣ‫▤ⴊޔ‬ოߩࠦ࡟ࠬ࠹ࡠ࡯࡞ࠍ⢄⤳ߦᚯߔ̌ༀ₹̍࡝ࡐⰮ⊕ߩ HDL ߣߩ㑆ߢ⢽⾰ࠍ੤឵ߐߖࠆⰮ⊕ߢ޽ࠆ‫ ߩߎޕ‬CETP ߇ߥ޿ࡑ࠙ࠬߩⴊਛߪ HDL-ࠦ࡟ࠬ࠹ࡠ࡯࡞߇߶ߣࠎߤߛ߇‫ޔ‬CETP߇޽ࠆࡅ࠻ߩⴊਛߢߪ LDL-ࠦ࡟ࠬ࠹ࡠ࡯࡞߇ᄙ޿‫߼ߚࠆ޽߇⊕Ⱞߩߎߪߦࠡࠨ࠙ޕ‬⋧ኻ⊛ߥ LDL-ࠦ࡟ࠬ࠹ࡠ࡯࡞㊂߇ᄙߊ‫ޔ‬LDL ฃኈ૕ࠍᰳ៊ߔࠆߣ㧔WHHL ࠙ࠨࠡ㧕‫ޔ‬ᄢ㊂ߩ LDL ߇ⴊਛߦṛ⇐ߒ‫ޔ‬LDL ฃኈ૕ࠍᰳ៊ߒߚࡅ࠻㧔ኅᣖᕈ㜞ࠦ࡟ࠬ࠹ࡠ࡯࡞
ⴊ∝㧕ߣࠃߊૃߚ⢽⾰ࡊࡠࡈࠔࠗ࡞ߣߥࠆ‫৻ޕ‬ᣇߢ‫ ߩࠬ࠙ࡑޔ‬LDL ฃኈ૕߇ᰳ៊ߒߡ
߽ LDL ࠍਥ૕ߣߒߚ⢽⾰ࡊࡠࡈࠔࠗ࡞ߦߪߥࠄߕ‫ޔ‬㜞⢽ⴊ∝ߩ⒟ᐲ߽シ޿‫ޔ߽ߦઁޕ‬ࠕࡐ࡝ࡐⰮ⊕(ࠕࡐ)B48 ߣ޿߁․ᱶߥⰮ⊕߇↥↢ߐࠇࠆ⤳ེ߇‫ߞߍޔ‬ᱤ㘃ߣࡅ࠻ߩ㑆ߢ⇣ߥࠆ߇‫ߩߘޔ‬ὐߢ߽࠙ࠨࠡߪࡅ࠻ߣ৻⥌ߒߡ޿ࠆ‫ޕ‬࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ࠙ࠨࠡߩ㐿⊒ߣᔕ↪
 ߎߩࠃ߁ߥ࠙ࠨࠡߩ೑ὐࠍ↢߆ߒ‫ޔ‬⢽⾰ઍ⻢࡮േ⣂⎬ൻߣߩ㑐ଥ߇␜ໂߐࠇࠆㆮવ
ሶߩᯏ⢻⸃ᨆࠍⴕ߁ߴߊ‫ࠢ࠶࠾ࠚࠫࠬࡦ࡜࠻ޔ‬㧔Tg㧕࠙ࠨࠡߩ㐿⊒߇ⴕࠊࠇߚ‫ޕ‬Tgࡑ࠙ࠬߩႎ๔߆ࠄㆃࠇࠆߎߣ 5 ᐕ㧔1985 ᐕ㧕‫ޔ‬Hammer ࠄߦࠃߞߡ Tg ࠙ࠨࠡߩೋ߼
ߡߩႎ๔߇ߥߐࠇߚ(8)‫ࠍࠡࠨ࠙ޕ‬฽߻ኅ⇓߳ߩㆮવሶᠲ૞ࠍ⋡⊛ߦⴕࠊࠇߚߎߩ⎇ⓥߢߪ‫ޔ‬ㆮવሶዉ౉ߦᚑഞߒߚ߽ߩߩⰮ⊕ߩ⊒⃻㊂߇ዋߥߊ‫ޔ‬᣿ࠄ߆ߥ⴫⃻ဳࠍ␜ߔ࠙
ࠨࠡߪᓧࠄࠇߥ߆ߞߚ‫߽ߢࠡࠨ࠙ޔࠄ߇ߥߒ߆ߒޕ‬ㆮવሶᡷᄌࡕ࠺࡞߇㐿⊒น⢻ߢ޽ࠆߎߣࠍ␜ߒߚ↹ᦼ⊛ߥႎ๔ߢ޽ߞߚ‫ ߩߘޕ‬9 ᐕᓟߩ 1994 ᐕ‫ޔ‬ᒰᤨࠞ࡝ࡈࠜ࡞࠾ࠕ
ᄢቇࠨࡦࡈ࡜ࡦࠪࠬࠦᩞ(UCSF) ߩࡐࠬ࠼ࠢߢ޽ߞߚ౒⪺⠪㧔▸ᳯᨋ㧕ߦࠃߞߡೋ߼
ߡ‫ޔ‬⢽⾰ઍ⻢㑐ㅪㆮવሶࠍዉ౉ߐࠇߚ Tg ࠙ࠨࠡ߇ႎ๔ߐࠇߡ޿ࠆ(9)‫ޔߚ߹ޕ‬หᤨᦼߦ UCSF ߦ⇐ቇߒߡ޿ߚጊਛિᒎ㧔2012 ᐕࡁ࡯ࡌ࡞↢ℂቇ࡮කቇ⾨ฃ⾨㧕߽ㆮવሶᡷᄌ࠙ࠨࠡߩ㐿⊒ߦ៤ࠊߞߡ޿ߚ‫ޕ‬ጊਛߪ▸ࠄߣߣ߽ߦ‫ߩ߳ࠬ࠙ࡑߣࠡࠨ࠙ޔ‬ㆮવሶᠲ૞ߦࠃࠅ⢽⾰⇣Ᏹ∝ߩㆮવሶᴦ≮ߩน⢻ᕈࠍតߞߚ߇‫ޔ‬ᴦ≮ലᨐࠍᦼᓙߒߡ⢄⤳ߦ⢽⾰ઍ⻢㑐ㅪㆮવሶ(APOBEC-1)ࠍዉ౉ߒߚ⚿ᨐ‫ޔ‬⣲≌߇⊒↢ߒߡߒ߹޿‫ߺ⹜ߩߘޔ‬ߪᄬᢌߦ⚳ࠊߞߡ޿ࠆ(10)‫ߩߘޕ‬ᓟ‫╳ޔ‬ᵄᄢቇߦ᜗⡜ߐࠇߚ▸ࠄߩࠣ࡞࡯ࡊ߇ਛᔃߣ
ߥߞߡᢙᄙߊߩ Tg ࠙ࠨࠡ߇ᧄ㇌ߢ㐿⊒ߐࠇ‫ޔ‬⢽⾰⇣Ᏹ∝߿േ⣂⎬ൻߩ⊒∝ߦ㊀ⷐߥᓎഀࠍᨐߚߔㆮવሶ࡮Ⱞ⊕߇᣿ࠄ߆ߦߐࠇߡ޿ߞߚ(1)‫ޕ‬Tg ࠙ࠨࠡߩ⎇ⓥ଀ 1㧦࡝ࡐⰮ⊕࡝ࡄ࡯࠯(LPL) Tg ࠙ࠨࠡ ⪺⠪㧔ዊᳰ㧕ߪ▸ߩ߽ߣߢୃ჻࡮ඳ჻⺖⒟ߩቇ↢ߣߒߡ‫ޔ‬Tg ࠙ࠨࠡߩ⎇ⓥߦ៤ࠊߞߚ‫ޕ‬ඳ჻⺰ᢥߩ⎇ⓥߢߪ‫ޔ‬⢽⾰ઍ⻢㉂⚛ߩ࡝ࡐⰮ⊕࡝ࡄ࡯࠯(LPL)߇ࠛࡀ࡞ࠡ࡯ઍ⻢߿ߘࠇߦᵷ↢ߒߚ♧ઍ⻢ߦߤߩࠃ߁ߥᓎഀࠍᨐߚߔ߆ࠍᬌ⸛ߒߚ‫ޔߦ․ޕ‬ⅣႺ࿃ሶߩᓇ
㗀߇ᄢ߈޿ߣߐࠇࠆࡋ࠹ࡠဳߩኅᣖᕈ㜞ࠦ࡟ࠬ࠹ࡠ࡯࡞ⴊ∝ᖚ⠪ࠍᮨߒߚࡋ࠹ࡠဳ
WHHL ࠙ࠨࠡߦ‫ޔ‬LPL ㆮવሶࠍዉ౉ߒ㧔LPL Tg WHHL ࠙ࠨࠡ㧕‫ޔ‬ⅣႺ⽶⩄ߣߒߡ㜞⢽⢌㘩ࠍᛩਈߒߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬LPL ㆮવሶࠍㆊ೾⊒⃻ߒߚ WHHL ࠙ࠨࠡߢߪ‫ޔ‬㜞⢽⢌㘩ࠍਈ߃ߡ߽‫ࠆߔ∝⊒ޔ‬⢈ḩߩ⒟ᐲ߇シߊ‫ޔ‬⢽⢌⚦⢩ߩ⢈ᄢ߇ᛥ೙ߐࠇߡ޿ߚ(࿑1)(11)‫ޔߦࠄߐޕ‬㜞⢽⢌㘩⽶⩄ߦ઻޿ㅴⴕߔࠆࠗࡦࠬ࡝ࡦᛶ᛫ᕈߩ⒟ᐲ߽シᐲߢ޽ߞߚ‫ޕ‬ߎߩ Tg ࠙ࠨࠡߢߪ‫ޔ‬chicken E-actin promoter ߦࠃߞߡోり⊛ߦ LPL ㆮવሶࠍㆊ೾
⊒⃻ߐߖߡ޿ߚ߇‫ߥ⚦⹦ޔ‬ᬌ⸛ߩ⚿ᨐ‫࡯ࠡ࡞ࡀࠛޔ‬ઍ⻢ߩਥⷐ⤳ེߢ޽ࠆ╭⡺ߢߩ LPL
ߩ⊒⃻㊂߇ᦨᄢߢ޽ߞߚ(11,12)‫ޔߦ߼ߚߩߎޕ‬LPL ߦࠃߞߡ࡝ࡐⰮ⊕ਛߩ࠻࡝ࠣ࡝࠮
࡝࠼߆ࠄಾࠅ಴ߐࠇߚㆆ㔌⢽⢌㉄߇‫ޔ‬⢽⢌⚵❱ࠃࠅ߽╭⡺ߦᄙߊขࠅㄟ߹ࠇ㉄ൻ࡮ᶖ⾌ߐࠇߚߎߣߢ‫ޔ‬⢈ḩߣࠗࡦࠬ࡝ࡦᛶ᛫ᕈߩᛥ೙ߦߟߥ߇ߞߚߎߣ߇␜ໂߐࠇߚ‫ޕ‬Tg ࠙ࠨࠡߩ⎇ⓥ଀ 2㧦C ෻ᔕᕈⰮ⊕(CRP) Tg ࠙ࠨࠡ C ෻ᔕᕈⰮ⊕(CRP)ߪ⥄ὼ఺∉ߦ㑐ࠊࠆฎౖ⊛ߥⰮ⊕(1929 ᐕ⊒⷗)ߛ߇‫ޔ‬ᕆᕈἻ∝ᤨߦߘߩỚᐲ߇ᕆỗߦ਄᣹ߔࠆߚ߼‫⃻ޔ‬࿷߽ߥ߅㍈ᢅߥἻ∝ࡑ࡯ࠞ࡯ߣߒߡ⥃ᐥߢ↪
޿ࠄࠇߡ޿ࠆ‫ ߒ߆ߒޕ‬2000 ᐕઍೋ㗡ࠃࠅ‫ޔ‬ᕆᕈἻ∝ߩߥ޿ੱߩⴊਛߦሽ࿷ߔࠆᓸ㊂ߩ CRP ࠍᬌ಴ߢ߈ࠆ ELISA ࠠ࠶࠻߇㐿⊒ߐࠇ‫ޔ‬ᓸ㊂ߩ CRP Ớᐲ߇㜞޿௑ะߦ޽ࠆ
ᖚ⠪ߢߪ‫ߩߘޔ‬ᓟߩᔃ╭᪪Ⴇ⊒∝₸߇㜞޿ߎߣ߇್᣿ߒ‫৻ޔ‬べ⣉శࠍᶎ߮ߚ‫ޔߦ․ޕ‬CRP ߣേ⣂⎬ൻߩ㑆ߦߤߩࠃ߁ߥ࿃ᨐ㑐ଥ߇޽ࠆߩ߆㧔ᔃ╭᪪Ⴇߩ࡝ࠬࠢߩ㜞޿ੱߩⴊਛߢ㜞୯ࠍ␜ߔࡑ࡯ࠞ࡯ߥߩ߆‫ޔ‬CRP ߇⋥ធ⊛ߦേ⣂⎬ൻ࡮ᔃ╭᪪Ⴇ⊒∝ߦ㑐ਈߒߡ޿ࠆߩ߆㧕߇ᵈ⋡ߐࠇ‫ޔ‬േ‛ታ㛎߇⸘↹ߐࠇߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬CRP ߪߍߞᱤ㘃ߦ߅޿ߡ߶ߣࠎߤ⊒⃻࡮ᯏ⢻߇ߥߊ‫ ߪߢ࡞࠺ࡕࠬ࠙ࡑޔ‬CRP ߣേ⣂⎬ൻߩ㑐ଥࠍ᣿ࠄ߆
ߦߢ߈ߥ޿ߎߣ߇ᜰ៰ߐࠇߡ޿ߚ‫৻ޕ‬ᣇ‫ޔߪߢࠡࠨ࠙ޔ‬CRP ߇ࡅ࠻ߣห᭽ߦἻ∝ߦࠃߞߡ⊒⃻߇⺃ዉߐࠇ‫↢ޔ‬ℂᯏ⢻߽᦭ߒߡ޿ࠆߚ߼‫ ߫ࠇ޿↪ࠍࠡࠨ࠙ޔ‬CRP ߣേ⣂⎬ൻߩ㑐ଥ߇᣿ࠄ߆ߦߥࠆߣ⠨߃‫ޔ‬ᬌ⸛ࠍⴕߞߚ‫ޔߕ߹ޕ‬ᱜᏱ࠙ࠨࠡߥࠄ߮ߦ 2 ⒳㘃ߩേ⣂⎬ൻ࠙ࠨࠡ㧔ࠦ࡟ࠬ࠹ࡠ࡯࡞㘩⽶⩄࠙ࠨࠡߣ WHHLMI ࠙ࠨࠡ㧕ߩⴊਛ CRP ࠍ᷹ቯߒߚߣߎࠈ‫ޔ‬ᱜᏱ࠙ࠨࠡߦᲧߴേ⣂⎬ൻ࠙ࠨࠡߢⴊਛ CRP ୯߇㜞ߊ‫ޔ‬േ⣂⎬ൻߩ⒟ᐲ߇ᦨ߽ᒝ޿ WHHLMI ࠙ࠨࠡߢߘߩ୯߇ᦨᄢߣߥߞߚ(࿑ 2)‫ޕ‬WHHLMI ࠙ࠨࠡߩേ
⣂⎬ൻ∛ᄌࠍ఺∉ᨴ⦡ߔࠆߣ‫ޔ‬CRP ߇∛ᄌౝߦᨴ߹ࠅ‫ޔ‬േ⣂⎬ൻ∛ᄌౝߦ CRP ߇ሽ࿷ߒߡ޿ࠆߎߣ߇᣿ࠄ߆ߦߥߞߚ(13)‫ߩߎޕ‬ᬌ⸛ߦࠃࠅ CRP ߣേ⣂⎬ൻߣߩኒធߥ㑐ଥ߇␜ߐࠇߚߚ߼‫ޔ‬ᰴߦ CRP Tg ࠙ࠨࠡߩ㐿⊒ߦࠃࠅ⋥ធ⊛ߥ࿃ᨐ㑐ଥ㧔CRP ߇േ⣂⎬ൻࠍ⊒↢࡮ㅴዷߐߖࠆߩ߆㧕ࠍᬌ⸽ߒߚ‫ޕ‬CRP ߪ↢ℂ⊛ߦߪ⢄⤳․⇣⊛ߥ⊒⃻ࠍ␜ߔߚ߼‫ ࠻ࡅޔ‬CRP ࠍ⢄⤳ߢ⊒⃻ߔࠆ Tg ࠙ࠨࠡߩ㐿⊒ࠍⴕ޿‫⃻⊒ޔ‬㊂ߩ⇣ߥࠆ 2 ♽⛔
ߩ૞⵾ߦᚑഞߒߚ‫࡞࡯ࡠ࠹ࠬ࡟ࠦߦࠄࠇߎޕ‬㘩ࠍਈ߃േ⣂⎬ൻࠍ⊒↢ߐߖߚߣߎࠈ‫ޔ‬ᄢേ⣂‫ޔ‬౰േ⣂ߩ޿ߕࠇߩേ⣂⎬ൻ߽‫ޔ‬ኻᾖ⟲ߣߩ㑆ߦ᦭ᗧߥᏅߪ⹺߼ࠄࠇߥ߆ߞߚ
(࿑ 2)‫∉఺ޕ‬ᨴ⦡ߩ⚿ᨐ‫ ࠻ࡅߩࠡࠨ࠙ࠢ࠶࠾ࠚࠫࠬࡦ࡜࠻ޔ‬CRP ߪേ⣂⎬ൻ∛ᄌߦᄢ㊂ߦᴉ⌕ߒߡ޿ߚ‫ߩߎޕ‬ᬌ⸛ߦࠃࠅ‫ޔ‬⢄⤳ߢ↥↢ߐࠇߚ CRP ߪⴊਛ߆ࠄേ⣂⎬ൻ∛ᄌ߳ߣṫ⌕ߔࠆ߇‫∛ޔ‬ᄌߩ⊒↢ㅴዷߦߪ߶ߣࠎߤᓇ㗀ߒߡߥ޿ߎߣ߇್᣿ߒ‫ޔ‬CRP ߪනߥࠆࡑ࡯ࠞ࡯Ⱞ⊕ߢ޽ࠆߎߣ߇᣿ࠄ߆ߦߥߞߚ(14)‫ޕ‬Tg ࠙ࠨࠡߩᦨㄭߩᚑᨐ㧦ࠕࡐ࡝ࡐⰮ⊕ A-II Tg ࠙ࠨࠡ ㄭᐕ‫ޔ‬⢽⾰⇣Ᏹ∝࡮േ⣂⎬ൻᡷༀߩᮡ⊛ߣߒߡ HDL ߇․ߦᵈ⋡ࠍ㓸߼ߡ޿ࠆ‫ޕ‬HDLߪ᛫േ⣂⎬ൻ૞↪ߩ޽ࠆ̌ༀ₹̍࡝ࡐⰮ⊕ߣߒߡࠃߊ⍮ࠄࠇ‫ߩߘޔ‬૞↪ߪਥߦࠕࡐ A-I(HDL ߩ᭴ᚑⰮ⊕)߇ᜂߞߡ޿ࠆ‫ޕ‬HDL ߦߪઁߦ߽ࠕࡐ A-II ߇ሽ࿷ߔࠆ߇‫ ࡐࠕޔ‬A-IߦᲧߴߘߩᯏ⢻ߪචಽߦ⸃᣿ߐࠇߡ޿ߥ߆ߞߚ‫ߩߘޕ‬⢛᥊ߦ߹ߚߒߡ߽⢽⾰ઍ⻢ߩ⒳Ꮕ߇޽ߞߚ‫ ࡐࠕޕ‬A-II ߩ⎇ⓥߪࡑ࠙ࠬߦࠃߞߡᢙᄙߊⴕࠊࠇߡ߈ߚ߇‫ߩࠬ࠙ࡑޔ‬⢽⾰ઍ⻢‫ ߦ․ޔ‬HDL ઍ⻢ߪ‫ ⴫ޔ‬1 ߦ␜ߔߏߣߊ‫ޔ‬CETP ߇ߥ޿ߎߣߢࡅ࠻ߣߪᄢ߈ߊ⇣ߥߞߡ޿ࠆ‫ ࡐࠕߩ࠻ࡅޔߪߦࠬ࠙ࡑޔߦࠄߐޕ‬A-II ߣߪ᭴ㅧ߽ᯏ⢻߽⇣ߥࠆౝ࿃ᕈࡑ
࠙ࠬࠕࡐ A-II ߇ሽ࿷ߒ‫ ࠍࠇߎޔ‬KO ߒߡ߽ࡅ࠻ࠕࡐ A-II ߩᓎഀߪಽ߆ࠄߥ޿‫ޔߚ߹ޕ‬ࡑ࠙ࠬߦㆮવሶዉ౉ࠍⴕߞߡ߽‫ߪߣ࠻ࡅޔ‬ᯏ⢻ߩ⇣ߥࠆࡑ࠙ࠬࠕࡐ A-II ߩỚᐲ߇ᄌേߒߡߒ߹޿‫ ࡐࠕ࠻ࡅޔ‬A-II ߩ૞↪ߛߌࠍ᣿ࠄ߆ߦߔࠆߎߣ߇࿎㔍ߢ޽ߞߚ(15)‫৻ޕ‬ᣇ‫ޔ‬࠙ࠨࠡߦߪ‫ ࡐࠕޔ‬A-II ߦ⋧ᒰߔࠆⰮ⊕߇⊒⃻ߒߡ޿ߥ޿‫ࠕ࠻ࡅߦࠡࠨ࠙ޔ߼ߚߩߘޕ‬ࡐ A-II ࠍ⊒⃻ߔࠆߎߣߢ‫ޔ‬㐳ࠄߊਇ᣿ߢ޽ߞߚࠕࡐ A-II ߩᯏ⢻߇᣿ࠄ߆ߦߥࠆߣ⠨߃‫⎇ޔ‬ⓥࠍ㐿ᆎߒߚ‫ ࡐࠕ࠻ࡅޕ‬A-II ߩ⊒⃻⺞▵♽߽฽߻ࠥࡁࡓ㈩೉ࠍ࠙ࠨࠡߩฃ♖ෆߦᵈ౉ߒ‫ ࡐࠕ࠻ࡅޔ‬A-II Tg ࠙ࠨࠡࠍ㐿⊒ߒߚ‫⃻⊒ޕ‬㊂ߩ⇣ߥࠆ 3 ♽⛔ߩ㐿⊒ߦᚑഞߒ‫ ࡐࠕޔߡ޿↪ࠍࠄࠇߎޔ‬A-II ߇⢽⾰ઍ⻢ߦ෸߷ߔᓇ㗀ߦߟ޿ߡᬌ⸛ࠍⴕߞߚ‫ߩߘޕ‬⚿ᨐ‫ ࡐࠕޔ‬A-II ߩⴊਛỚᐲ߇㜞୯ߦߥࠆ߶ߤ‫ⴊޔ‬ਛߩ࠻࡝ࠣ࡝࠮࡝࠼(TG)ߣ✚ࠦ࡟ࠬ࠹ࡠ࡯࡞(TC) ߇㜞୯ߦߥࠅ‫৻ޔ‬ᣇߢ HDL-ࠦ࡟ࠬ࠹ࡠ࡯࡞(HDL-C)ߪૐਅߒߚ‫߽ᦨޕ‬⊒⃻㊂ߩ㜞޿♽⛔ߩⴊਛࠕࡐ A-II Ớᐲ߇ 30 mg/dl ⒟ᐲߢ޽ࠅ‫ޔ‬ஜᏱࡅ࠻ߩᐔဋ୯ߣ
৻⥌ߒߡ޿ߚߚ߼‫⸃ߊߒ⹦ߡ޿↪ࠍ⛔♽ߩߎޔ‬ᨆߒߚ‫ޕ‬㓽㓶ߩ࠙ࠨࠡࠍ↪޿ߡ‫⛘ޔ‬㘩ᤨߣ៨㘩ᓟߢⴊਛ⢽⾰ࠍ᷹ቯߒߚߣߎࠈ‫ ࡐࠕ߽ߡ޿߅ߦࠇߕ޿ޔ‬A-II Tg ࠙ࠨࠡߢߪ
TC‫ޔ‬TG‫ࡦ࡝ޔ‬⢽⾰߇㜞୯ࠍ␜ߒ‫ޔ‬HDL-ࠦ࡟ࠬ࠹ࡠ࡯࡞߇ૐ୯ߢ޽ࠅ‫ޔ‬⢽⾰⇣Ᏹ∝ߣ๭߫ࠇࠆേ⣂⎬ൻᗖ⿠ᕈߩ∛ᘒ߇↢ߓߡ޿ߚ(࿑ 3)(16)‫ޔࠄ߇ߥߒ߆ߒޕ‬2 ᐕ㑆ߩ㐳ᦼⷰኤࠍⴕߞߚ⚿ᨐ‫ ࡐࠕޔ‬A-II Tg ࠙ࠨࠡߢߪേ⣂⎬ൻࠍ⥄ὼ⊒∝ߒߥ߆ߞߚ‫ޔߢߎߘޕ‬㘩㙄ߢߩࠦ࡟ࠬ࠹ࡠ࡯࡞⽶⩄ߦࠃࠅേ⣂⎬ൻࠍ⊒↢ߐߖ‫ ࡐࠕޔ‬A-II ߩേ⣂⎬ൻߦኻߔࠆᓎഀࠍᬌ⸛ߒߚ(17)‫࡞࡯ࡠ࠹ࠬ࡟ࠦޕ‬ᛩਈᦼ㑆ਛߩ⢽⾰୯ߪ‫ޔ‬Tg ࠙ࠨࠡߢ TG ߇㜞޿௑ะߦ޽ߞߚ߇ TC ߣ HDL-C ߦ㗼⪺ߥᏅߪ⹺߼ࠄࠇߥ߆ߞߚ‫ޕ‬16 ㅳ㑆ߩࠦ࡟ࠬ࠹
ࡠ࡯࡞ᛩਈ⚳ੌᓟ‫ޔ‬ᄢേ⣂∛ᄌࠍ⢽⢌ᨴ⦡ߒߚߣߎࠈ‫ ࡐࠕޔ‬A-II ࠙ࠨࠡߢߪേ⣂⎬ൻ∛ᄌ߇⪺᣿ߦዋߥ޿ߎߣ߇᣿ࠄ߆ߦߥߞߚ(࿑ 4)‫ޕ‬౰േ⣂⎬ൻߦࠃࠆ⁜⓰₸߽ Tg ࠙ࠨࠡߢシᐲߥ௑ะߦ޽ࠅ‫ޔ‬േ⣂⎬ൻ∛ᄌࠍ఺∉ᨴ⦡ߒߚߣߎࠈ∛ᄌౝߦࠕࡐ A-II ߇ᴉ⌕ߒߡ޿ߚ‫ోޔߚ߹ޕ‬り⊛ߥἻ∝⁁ᘒߩᜰᮡߣߒߡⴊਛ CRP ࠍ⸘᷹ߔࠆߣ‫ޔ‬ኻᾖ⟲ߢߪࠦ࡟ࠬ࠹ࡠ࡯࡞ᛩਈᓟ⪺᣿ߦჇടߒἻ∝⁁ᘒߩᗖ⿠߇␜ໂߐࠇߚ߇‫ ࡐࠕޔ‬A-II ࠙ࠨࠡߢߪᛩਈᓟ߽ CRP ୯߇ૐ୯ߩ߹߹ߢ޽ߞߚ‫ޕ‬᛫㉄ൻ⢻ߩᜰᮡߣߥࠆⴊਛ Paraoxonaseᵴᕈ߽ࠕࡐ A-II ࠙ࠨࠡߩ߶߁߇㜞୯ߢ޽ߞߚ‫⚿ߩࠄࠇߎޕ‬ᨐ߆ࠄ‫ ࡐࠕޔ‬A-II ߦߪᒝജߥ᛫Ἳ∝࡮᛫㉄ൻ࡮᛫േ⣂⎬ൻ૞↪߇޽ࠆߎߣ߇ផኤߐࠇߚߚ߼‫ ࡐࠕޔ‬A-II ߇ HDLߩᯏ⢻ߦߤߩࠃ߁ߥᄌൻࠍ෸߷ߒߚ߆ࠍ in vitro ߦߡᬌ⸛ߒߚ‫⚦♽ࠫ࡯ࠔࡈࡠࠢࡑޕ‬⢩ᩣߩ U937 ߦ࡝ࡐᄙ♧(LPS)ࠍᛩਈߒἻ∝ࠍᗖ⿠ߐߖࠆታ㛎♽ߦ‫ޔ‬Tg ࠙ࠨࠡߣኻᾖ
⟲߆ࠄណขߒߚ HDL ࠍᷝടߒ᛫Ἳ∝૞↪ࠍᬌ⸛ߒߚ⚿ᨐ‫ޔ‬ਔ⟲ߩ HDL ߇ࡑࠢࡠࡈࠔ
࡯ࠫ߆ࠄߩἻ∝ᕈࠨࠗ࠻ࠞࠗࡦߩಽᴲࠍ⪺᣿ߦᛥ೙ߒߚ‫ޕ‬2 ⟲㑆ߢߘߩലᨐࠍᲧセߔࠆߣ‫ ࡐࠕޔ‬A-II ࠙ࠨࠡ↱᧪ߩ HDL ߢࠃࠅᒝ޿᛫Ἳ∝૞↪߇⹺߼ࠄࠇߚ‫ ߚ߹ޕ‬HDLߩ⋥ធ⊛ߥ᛫േ⣂⎬ൻ૞↪ߣߒߡ‫∛ޔ‬ᄌ߆ࠄߩࠦ࡟ࠬ࠹ࡠ࡯࡞ᒁ߈ᛮ߈⢻ߦߟ޿ߡ߽
ਔ⟲ߩ HDL ߢᬌ⸛ߒ‫ ࡐࠕޔ‬A-II ࠙ࠨࠡߩ HDL ߢࠦ࡟ࠬ࠹ࡠ࡯࡞ᒁ߈ᛮ߈⢻߇㜞޿ߎߣ߇᣿ࠄ߆ߦߥߞߚ‫ ࡐࠕޕ‬A-I ߩࠦ࡟ࠬ࠹ࡠ࡯࡞ᒁ߈ᛮ߈ߦߪ ABCA1 ߣ๭߫ࠇࠆ
⢽⾰ォㅍⰮ⊕߇ᔅ㗇ߣߥࠆ߇‫ޔ‬ABCA1 ࠍᒝ೙⊒⃻ߐߖߚ⚦⢩ታ㛎ߥࠄ߮ߦ ABCA1
㒖ኂ೷ߩᛩਈታ㛎ߦࠃࠅ‫ ࡐࠕޔ‬A-II ߩࠦ࡟ࠬ࠹ࡠ࡯࡞ᒁ߈ᛮ߈ߦ߽ ABCA1 ߇㑐ਈߒߡ޿ࠆߎߣ߇್᣿ߒߚ‫ ࡐࠕޔߚ߹ޕ‬A-II ߪ㜞⢽ⴊ∝ᖚ⠪ߦ߅޿ߡ HDL એᄖߩ࡝ࡐⰮ
⊕ߦ߽ሽ࿷ߒߡ޿ࠆߎߣ߇⍮ࠄࠇߡ߅ࠅ‫ࠍ࡞࡯ࡠ࠹ࠬ࡟ࠦޔ‬ᛩਈߐࠇߚࠕࡐ A-II ࠙ࠨࠡߢ߽‫ޔ‬HDL એᄖߩേ⣂⎬ൻᗖ⿠ᕈߩ޽ࠆ࡝ࡐⰮ⊕ߦዋ㊂ߥ߇ࠄࠕࡐ A-II ߇ሽ࿷ߒߡ޿ߚߚ߼‫㉄ߩ⊕Ⱞࡐ࡝ߩࠄࠇߎޔ‬ൻᗵฃᕈࠍᬌ⸛ߒߚ‫࡞࡯ࡠ࠹ࠬ࡟ࠦޔߣࠆߔޕ‬ᛩਈ࠙ࠨࠡߢᦨ߽ᒝ޿േ⣂⎬ൻᗖ⿠ᕈࠍᜬߟǪ-VLDL ߦ߅޿ߡ‫ ࡐࠕޔ‬A-II ࠍ฽߻Ǫ
-VLDL ߪ㉄ൻߐࠇߦߊ޿ߎߣ߇್᣿ߒߚ‫⚿ߩࠄࠇߎޕ‬ᨐ߆ࠄ‫ߩߘߢ߹ࠇߎޔ‬ᓎഀ߇ਇ᣿ߢ޽ߞߚࠕࡐ A-II ߦ⪺᣿ߥ᛫േ⣂⎬ൻ૞↪߇޽ࠆߎߣ߇ೋ߼ߡ᣿ࠄ߆ߦߐࠇߚ‫ޕ‬Tg ࠙ࠨࠡߩዷᦸ ਄ㅀߩࠃ߁ߦ‫ޔ‬⢽⾰ઍ⻢♽߇ࡅ࠻ߣࠃߊ㘃ૃߒߚ࠙ࠨࠡࡕ࠺࡞ࠍᵴ↪ߒߡ‫ޔ‬⢽⾰ઍ⻢߿േ⣂⎬ൻ߳ߩ૞↪߇ᧂ⍮ߩㆮવሶࠍ⸃ᨆߔࠆߎߣߢ‫ߥ⋉᦭ޔ‬⍮⷗߇ᓧࠄࠇࠆߎߣ
ࠍታ⸽ߒߡ߈ߚ‫ޔߚ߹ޕ‬േ‛⒳ߩ㆑޿ߪ‫⚿ޔ‬ᨐߦᄢ߈ߊᓇ㗀ߔࠆ႐ว߇޽ࠆ‫ ⴫ޕ‬2 ߦ␜ߔߏߣߊ‫ޔ‬ห৻ߩㆮવሶࠍㆊ೾⊒⃻ߒߚ Tg ࡕ࠺࡞ߢ߽‫ߢࠡࠨ࠙ߣࠬ࠙ࡑޔ‬േ⣂⎬ൻߦኻߔࠆ૞↪߇ᱜ෻ኻߢ޽ߞߚߣ޿߁੐଀߇ⶄᢙႎ๔ߐࠇߡ޿ࠆ‫ޔ߽ࠄ߆ߣߎߩߎޕ‬⎇ⓥ⋡⊛ߦᔕߓߚേ‛⒳ߩㆬᛯߩ㊀ⷐᕈ߇ౣ⹺⼂ߐࠇࠆ‫੹ޕ‬࿁ߩࠕࡐ A-II ߇␜ߒߚ⪺᣿ߥ᛫േ⣂⎬ൻ૞↪ߪ‫੹ޔ‬ᓟ‫ࠆߥࠄߐޔ‬ᬌ⸛ࠍ㊀ߨ‫ޔ‬ᣂߚߥേ⣂⎬ൻߩ੍㒐࡮ᴦ≮ᴺߩ㐿⊒߳ߣ⊒ዷߐߖࠆߎߣߦࠃࠅ‫߳࠴࡯ࠨ࡝࡞࠽࡚ࠪ࡯࡟ࠬࡦ࡜࠻ߩ࡞࠺ࡕࠡࠨ࠙ޔ‬ߩ᦭↪ᕈߦߟ޿ߡ߽ㅊ᳞ߒߡ޿߈ߚ޿‫ޕ‬1.

Fan, J., and Watanabe, T. (2003) Pharmacol Ther 99, 261-282

2.

Ignatowski, A. (1908) Arch. Med. Exp. Anat. Pathol. 20, 1-20

3.

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4.

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1-9

5.

Shiomi, M., Koike, T., and Ito, T. (2013) Atherosclerosis 231, 39-47

6.

Goldstein, J. L., and Brown, M. S. (2009) Arterioscler Thromb Vasc Biol 29, 431-438

7.

㆙⮮┨. (1980) ↢ൻቇ 52, 1033-1049

8.

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M., Palmiter, R. D., and Brinster, R. L. (1985) Nature 315, 680-683

9.

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10.

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Taylor, J. M., and Innerarity, T. L. (1995) Proc Natl Acad Sci U S A 92, 8483-8487

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7521-7529

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Itamoto, K., Watanabe, T., Tsutsumi, K., Yamada, N., and Fan, J. (2004)

Diabetologia 47, 1202-1209
13.

Sun, H., Koike, T., Ichikawa, T., Hatakeyama, K., Shiomi, M., Zhang, B., Kitajima,S., Morimoto, M., Watanabe, T., Asada, Y., Chen, Y. E., and Fan, J. (2005) Am J

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C.,

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Lipid Res 39, 457-462
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Watanabe, T., and Fan, J. (2009) Arterioscler Thromb Vasc Biol 29, 2047-2053

17.

Wang, Y., Niimi, M., Nishijima, K., Waqar, A. B., Yu, Y., Koike, T., Kitajima, S., Liu,E., Inoue, T., Kohashi, M., Keyamura, Y., Yoshikawa, T., Zhang, J., Ma, L., Zha, X.,
Watanabe, T., Asada, Y., Chen, Y. E., and Fan, J. (2013) Arterioscler Thromb Vasc

Biol 33, 224-231࿑1. LPL䈱᛫⢈ḩലᨐ

⴫1. 䉡䉰䉩䈱⢽⾰ઍ⻢♽䈱䊍䊃䈫䈱㘃ૃὐ

CETP
apoB✬㓸㉂⚛
((apobec-1)
p
)

䊙䉡䉴

䉡䉰䉩

䊍䊃

䈭䈚

䈅䉍

䈅䉍

ዊ⣺䋯⢄⤳

ዊ⣺

ዊ⣺

chylomicrons
VLDL

chylomicrons

chylomicrons

LDLฃኈ૕⺞▵Ᏹ䈮㜞⊒⃻䊐䉞䊷䊄䊋䉾䉪೙ᓮ䊐䉞䊷䊄䊋䉾䉪೙ᓮ

LDLฃኈ૕䊖䊝ᰳ៊ⴊਛ䉮䊧䉴䊁䊨䊷䊦(mg/dl)200-270(LDLR KO)

500-1000(WHHL)

500-1000(FH)

apoB-48(Fan and Watanabe, 2003 ᡷᄌ)(LPL Tg WHHL䉡䉰䉩)

Subcutaneous

Visceral

Total

LPLㆊ೾⊒⃻䈮䉋䉍䇮WHHL䉡䉰䉩䈱㜞⢽⢌㘩ᛩਈ䈮䉋䉎⢈ḩ䉇䉟䊮䉴䊥䊮ᛶ᛫ᕈ䈱⊒∝䉕ᛥ೙

࿑2. CRP䈱േ⣂⎬ൻ䈮䈍䈔䉎ᓎഀ(Koike,et al., 2004)

࿑3. 䉝䊘A-II Tg䉡䉰䉩䈱⢽⾰⇣Ᏹ∝䋨CRP Tg䉡䉰䉩䋩

Triglycerides(TG)䉥䉴
non-Tg (n=12)(mg/dl)
300

200

**

Total cholesterol(TC)(mg/dl)
120

160

*

*

Phospholipids(mg/dl)

80

**

**

HDL-C
(mg/dl)
30

120
20

**

80

A-II Tg (n=10)

**

10

40

100

40
0

Fasted

Fed

**

**

(mg/dl)䊜䉴
non-Tg (n=15)

350
300

0

AII-Tg (n=14)േ⣂⎬ൻ䉡䉰䉩䈱ⴊਛCRP䈏㜞䈇

160

**

Fasted

0

Fed

Fed

(mg/dl)
40

250
200

Fasted

**

**

30

150
20

80

150

100
40

0
Fasted

Fed

0

Fed

**

0

0
Fasted

**

10

50

50

non-Tg0(mg/dl)

**

120

100

䉡䉰䉩䈱േ⣂⎬ൻ∛ᄌ䈮CRPᴉ⌕CRP䈱േ⣂⎬ൻ䈮ኻ䈜䉎⋥ធ૞↪䉕Tg䈮䈩ᬌ⸽䈚䇮․Ბ䈱ᓇ㗀䉕ਈ䈋䈩䈇䈭䈇䈖䈫䈏್᣿䋨ోり䈱Ἳ∝⁁ᘒ䉕෻ᤋ䈚䈢䊙䊷䉦䊷䈮䈜䈑䈭䈇䋩Fed(mg/dl)250200

వⴕ⎇ⓥ(Sun and Koike et al., 2005)

FastedFastedFed

FastedFed2ᐕ䈱㐳ᦼⷰኤ䈱⚿ᨐ䇮േ⣂⎬ൻ䉕⥄ὼ⊒∝䈞䈝

hCRP-Tg(Koike and Kitajima et al., 2009)

TG, triglycerides, TC, total cholesterol, PL, phospholipids

Tg䉡䉰䉩

࿑4. 䊍䊃䉝䊘A-II䈏േ⣂⎬ൻ䉕ᛥ೙

(Koike and Kitajima et al., 2009)⴫2. 䊃䊤䊮䉴䉳䉢䊆䉾䉪䊙䉡䉴䈫䉡䉰䉩䈱⴫⃻ဳ䈱ਵ㔌
⢽⾰ઍ⻢㑐ㅪ
ㆮવሶ

䊙䉡䉴

䉡䉰䉩

Hepatic lipaseേ⣂⎬ൻ ଦㅴ

േ⣂⎬ൻ ᛥ೙

LCATേ⣂⎬ൻ ଦㅴ

േ⣂⎬ൻ ᛥ೙

15Ͳlipoxygenaseേ⣂⎬ൻ ଦㅴ

േ⣂⎬ൻ ᛥ೙

apoE3

േ⣂⎬ൻ ᛥ೙

േ⣂⎬ൻ ଦㅴ

Human apoAͲIIേ⣂⎬ൻ ଦㅴ

േ⣂⎬ൻ ᛥ೙

⢄⤳䈪วᚑ䈘䉏䈢䊍䊃䉝䊘A-II䈏∛ᄌౝ䈮೔㆐
(Wang and Niimi et al., 2013)

LCAT, Lecithin:cholesterol acyltransferase(Fan and Watanabe, 2003 ᡷᄌ)‫ޟ‬㜞ᵴᕈဳ TALEN ߩ㐿⊒ߣ᭽‫ߥޘ‬േ‛ߦ߅ߌࠆࠥࡁࡓ✬㓸‫ޠ‬ጊᧄ ථ㧔ᐢፉᄢቇ࡮ᄢቇ㒮ℂቇ⎇ⓥ⑼࡮ᢙℂಽሶ↢๮ℂቇኾ᡹㧕 ߎࠇ߹ߢᄙߊߩേ‛ߦ߅޿ߡ‫⚦ޔ‬⢩ౝ޽ࠆ޿ߪ↢‛୘૕ౝߦ߅޿ߡ⋡⊛ߩㆮવሶߩߺࠍᡷᄌ㧔ㆮવሶࡁ࠶ࠢࠕ࠙࠻޽ࠆ޿ߪㆮવሶࡁ࠶ࠢࠗࡦ㧕ߔࠆᛛⴚߪ
⏕┙ߒߡ޿ߥ߆ߞߚ‫ ߪߢࠬ࠙ࡑޕ‬ES ⚦⢩ࠍ↪޿ߡ⋡⊛ߩㆮવሶࠍᡷᄌߒ‫ޔ‬ᡷᄌߒߚ ES ⚦⢩ࠍ೑↪ߒߚ୘૕૞⵾ᴺ߇⏕┙ߒߡ޿ࠆ߇‫ߩઁޔ‬േ‛ߢߪߎߩᣇᴺ߇
࿎㔍ߥߚ߼ൻቇᄌ⇣ේ߿࠻࡜ࡦࠬࡐ࠱ࡦߢࠥࡁࡓㆮવሶߦ࡜ࡦ࠳ࡓߦᄌ⇣ࠍዉ
౉ߔࠆᣇᴺ߇↪޿ࠄࠇߡ߈ߚ‫ߥࡓ࠳ࡦ࡜ޔࠄ߇ߥߒ߆ߒޕ‬ᄌ⇣ࠍዉ౉ߐࠇߚ㓸࿅߆ࠄ⋡⊛ߩㆮવሶࠍᡷᄌߒߚᄌ⇣૕ࠍㆬ೎ߔࠆ૞ᬺߪᄙᄢߥഭജࠍᔅⷐߣߔ
ࠆߚ߼‫ޔ‬ㆮવሶᡷᄌߪ㘺⢒߇ኈᤃߢᄌ⇣૕ࠬࠢ࡝࡯࠾ࡦࠣ߇น⢻ߥ㒢ࠄࠇߚേ‛ࠍਛᔃߦⴕࠊࠇߡ߈ߚ‫⁁ߥ߁ࠃߩߎޕ‬ᴫߩਛ‫ޔ‬છᗧߩ㈩೉ߦኻߒߡ⸳⸘น⢻ߥ ੱ Ꮏ ೙ 㒢 ㉂ ⚛ 㧔 ੱᎿ ࠿ ࠢ ࡟ ࠕ ࡯ ࠯㧕ߩ Zinc-finger nuclease㧔 ZFN 㧕 ߿Transcription activator-like effector nuclease㧔TALEN㧕ࠍ↪޿ߚㆮવሶᡷᄌ
ᛛⴚ㧔ࠥࡁࡓ✬㓸ᛛⴚ㧕߇㐿⊒ߐࠇ‫ޔ‬ၮ␆߆ࠄᔕ↪߹ߢߩᐢ޿ಽ㊁ߢߩ೑↪߇ᦼᓙߐࠇߡ޿ࠆ‫✬ࡓࡁࠥޕ‬㓸ᛛⴚߪ‫ੱޔ‬Ꮏ࠿ࠢ࡟ࠕ࡯࠯ࠍ⚦⢩߿ฃ♖ෆߦዉ౉ߔࠆߛߌߢน⢻ߥߎߣ‫ޔ‬ේℂ⊛ߦోߡߩ↢‛ߦ೑↪น⢻ߢ޽ࠆߎߣ߆ࠄ‫ޔ‬ᰴ਎ઍߩㆮવሶᡷᄌᛛⴚߣ⠨߃ࠄࠇߡ޿ࠆ‫ᧄߦࠄߐޕ‬ᐕೋ߼ߦߪ‫ ╙ޔ‬3 ਎ઍߩࠥࡁ
ࡓ✬㓸ᛛⴚߣߒߡ CRISPR/Cas ࠪࠬ࠹ࡓ߇⊒⴫ߐࠇ‫ߩߘޔ‬ᣇᴺߩ◲ଢߐߦᄙߊ
ߩ⎇ⓥ⠪߇㛳޿ߚ‫ޕ‬ ᧄ⻠Ṷߢߪ‫ޔ‬TALEN ࠍၮ⋚ߣߔࠆࠥࡁࡓ✬㓸ᛛⴚࠍ↪޿ߚ᭽‫ߥޘ‬േ‛ߢߩㆮવሶᡷᄌߩ⃻⁁ߣน⢻ᕈߦߟ޿ߡ⚫੺ߔࠆ‫ޕ‬2010 ᐕߦ TALEN ߇ೋ߼ߡႎ๔ߐ
ࠇߡએ᧪‫ޔ‬᭽‫ߥޘ‬േ‛㧔࠯ࡉ࡜ࡈࠖ࠶ࠪࡘ߿ࠞࠛ࡞ߥߤ㧕ߢߩㆮવሶࡁ࠶ࠢࠕ
࠙࠻߇ᚑഞߔࠆ৻ᣇ‫ᦼߪߢ࠻࠶࡜߿ࠬ࠙ࡑޔ‬ᓙߐࠇࠆ㜞޿ല₸ߢߩᄌ⇣ዉ౉߇ታ⃻ߒߡ޿ߥ߆ߞߚ‫ߢߎߘޕ‬ᚒ‫ޔߪޘ‬Voytas ࡜ࡏߩ Golden Gate ᴺࠍᡷ⦟ߒ‫ޔ‬TALE ߩ N ᧃ┵ߣ C ᧃ┵ࠍᰳᄬߐߖߚ TALEN-NC ߩല₸⊛૞⵾ᣇᴺ㧔6 ࡕࠫ
ࡘ࡯࡞ᴺ㧕ߩ㐿⊒ߣၭ㙃⚦⢩ߢߩ⹏ଔᣇᴺࠍ⏕┙ߒߡ߈ߚ㧔Sakuma et al.,2013㧕‫ޔߦࠄߐޕ‬TALEN-NC ߣ Exonuclease I ߣߩ౒ዉ౉ߦࠃߞߡ࡜࠶࠻ߢߩ
ᄌ⇣ዉ౉ߦᚑഞߒߚ㧔੩ㇺᄢቇ࡮⌀ਅవ↢ߩᚑᨐ, Mashimo et al., 2013㧕‫߆ߒޕ‬ߒߥ߇ࠄ‫ޔ‬TALEN-NC ߩߺߩࠗࡦࠫࠚ࡚ࠢࠪࡦߢ࡜࠶࠻୘૕߳ߩᄌ⇣ዉ౉߇⏕⹺ߢ߈ߕ‫ࠆߥࠄߐޔ‬ᡷ⦟߇ᔅⷐߣ⠨߃ࠄࠇߚ‫੹ޕ‬࿁‫ޔ‬TALEN ߩ DNA ⚿วࡕࠫࡘ࡯࡞ߦᡷ⦟ࠍട߃ߚ Platinum Gate ᴺࠍ⏕┙ߒ‫ޔ‬㜞ᵴᕈဳߩ Platinum
TALEN ࠍ↪޿ߡၭ㙃⚦⢩߅ࠃ߮ࠞࠛ࡞‫ߩߢࠬ࠙ࡑޔ‬ᄌ⇣ዉ౉ല₸ࠍ⺞ߴߚߩߢ‫ޔ‬ߘߩ⚿ᨐߦߟ޿ߡ⚫੺ߔࠆ‫ޕ‬Platinum TALEN ߪၭ㙃⚦⢩ߩ SSA ᵴᕈ⹏ଔߦ߅
޿ߡ‫ߢ߹ࠇߎޔ‬૞⵾ߒߡ޿ߚ Voytas TALEN ߩ 1.5~2.5 ୚ߩ㜞޿ᵴᕈࠍ᦭ߒ‫ޔ‬ၭ㙃⚦⢩߅ࠃ߮୘૕ߦ߅޿ߡ߽㜞޿ᄌ⇣ዉ౉ࠍ␜ߔߎߣ߇᣿ࠄ߆ߦߥߞߚ‫․ޕ‬ߦࠞࠛ࡞ߦ߅޿ߡߪ F0 ߦ߅޿ߡቢోߥㆮવሶ⎕უ୘૕ࠍᓧࠆߎߣߦᚑഞߒߚ
㧔Suzuki et al., 2013㧕‫ޕ‬[ᒁ↪ᢥ₂]
Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai H,
Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A,
Hayashi S and Yamamoto T. Efficient TALEN construction and evaluation
methods for human cell and animal applications. Genes Cells, 18: 315-326(2013)
Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B,
Yamamoto T and Serikawa T. Efficient gene targeting by TAL effector
nucleases coinjected with exonucleases in zygotes. Scientific Reports, 3,1253 (2013)
Suzuki KI, Isoyama Y, Kashiwagi K, Sakuma T, Ochiai H, Sakamoto N,
Furuno N, Kashiwagi A and Yamamoto T, High efficiency TALENs enableF0 functional analysis by targeted gene disruption in Xenopus laevis.

Biology Open, 2, 448-452 (2013)ੱᎿ࠿ࠢ࡟ࠕ࡯࠯ߪ‫ޔ‬છᗧߩ㈩೉ߦ DNA ੑᧄ㎮ಾᢿ㧔DSB㧕ࠍዉ౉ߔࠆੱᎿ೙㒢㉂⚛ߢ޽ࠆ‫ޕ‬ਥߦ‫ޔ‬ZFN ߣ TALEN ߩ 2 ⒳㘃ߩੱᎿ࠿ࠢ
࡟ࠕ࡯࠯߇ᮡ⊛ㆮવሶߩᡷᄌߦ૶ࠊࠇߡ޿
ࠆ‫ޕ‬ੱᎿ࠿ࠢ࡟ࠕ࡯࠯ߦࠃߞߡಾᢿߐࠇߚ
DNA ߩୃᓳㆊ⒟ߦ߅޿ߡ‫ޔ‬ᄌ⇣ዉ౉߿࠲࡯ࠥ
࠹ࠖࡦࠣߥߤߩࠥࡁࡓ✬㓸߇น⢻ߣߥࠆ‫ޕ‬

TALEN ߪ‫ޔ‬૞⵾߇Ყセ⊛◲ଢߢ޽ࠆߎߣ߆
ࠄ‫✬ࡓࡁࠥޔ‬㓸ߦᐢߊ૶ࠊࠇߡ޿ࠆ‫ޕ‬૞⵾ߩߚ߼ߩࠝ࡯ࡊࡦ࡝࠰࡯ࠬ߇ឭଏߐࠇߡ޿ࠆ‫ޕ‬ᚒ‫⎇ߩޘ‬ⓥቶߢߪ‫ޔ‬TALEN ૞⵾ߩ 6 ࡕࠫࡘ࡯࡞ㅪ⚿ᴺߣၭ㙃⚦⢩ߢߩᵴᕈ⹏ଔᴺࠍ⏕┙ߒߡ߈ߚ‫ޕ‬૞⵾ߒߚ TALEN ߩࠞࠛ࡞ߢߩലᨐࠍ⺞ߴߚߣߎࠈ‫ޔ‬㕖Ᏹߦ㜞޿ᄌ⇣ዉ౉߇⏕⹺ߐࠇߚ‫ޕ‬૞⵾ߒߚ TALEN ߣ ExoI ߣߩ౒ዉ౉ߦࠃߞ
ߡല₸⊛ߦᄌ⇣ዉ౉߇น⢻ߣߥߞߚ‫ߦࠄߐޕ‬㜞ᵴᕈဳ TALEN㧔Platinum TALEN㧕ߦࠃߞߡ‫ޔ‬F0 ࡁ࠶ࠢࠕ࠙࠻߽น⢻ߣߥߞߡ޿ࠆ‫ޕ‬

CRISPR/Cas ࠪࠬ࠹ࡓࠍ↪޿ߚࠥࡁࡓ✬㓸ᛛⴚ߇ᧄᐕᆎ߼ߦႎ๔ߐࠇ‫◲ߩߘޔ‬ଢߐ߆ࠄᵈ⋡ߐࠇߡ޿ࠆ‫ޕ‬

CRISPR/Cas ࠪࠬ࠹ࡓߦࠃߞߡࡑ࠙ࠬ ES߿୘૕ߦ߅޿ߡⶄᢙหᤨㆮવሶ⎕უ߇ႎ๔ߐ
ࠇߚ‫ޕ‬ࠥࡁࡓ✬㓸ࠍ↪޿ߚㆮવሶᡷᄌᛛⴚ߇੹ᓟ
࡜ࠗࡈࠨࠗࠛࡦࠬߩၮ⋚ᛛⴚߣߥߞߡ޿ߊน
⢻ᕈ߇㜞޿‫ޕ‬ࠥࡁࡓ✬㓸ᛛⴚࠍ‫ޔ‬࿖ౝߩၮ␆⎇ⓥ߅ࠃ߮
ᔕ↪⎇ⓥߦⓍᭂ⊛ߦ૶ߞߡ޿ߊߎߣ߇㊀ⷐߢ޽ࠆ‫ޕ‬ࠥࡁࡓ✬㓸ࠦࡦ࠰࡯ࠪࠕࡓߢߪ‫ޔ‬ᛛⴚ⻠⠌ળ߿⎇ⓥળࠍㅢߒߡ‫ޔ‬࿖ౝߢߩࠥࡁࡓ✬㓸⎇ⓥߩ࡟ࡌ࡞ࠕ࠶ࡊࠍᡰេߒߡ޿ࠆ‫ޕ‬㧨ߘߩઁ㧪

㑐⷏ታ㛎േ‛⎇ⓥળߛࠃࠅ 㑐⷏ታ㛎േ‛⎇ⓥળ╙ 34 ภߦឝタߒߚ╙ 114 ࿁⎇ⓥળએᓟ‫ޔ‬એਅߩ⎇ⓥળ߇㐿௅ߐࠇߚ‫ޕ‬㧝㧕╙ 115 ࿁⎇ⓥળ㧔ᐔᚑ 24 ᐕ 9 ᦬ 7 ᣣ㧔㊄㧕‫ޔ‬ᄢ㒋ᄢቇ ㌁᧙ળ㙚㧕㧨⻠Ṷળ㧪 ࠹࡯ࡑ㧦േ‛ታ㛎ታᣉ૕೙ߩ⺖㗴㧝㧚ࠊ߇࿖ߩേ‛ታ㛎ታᣉ૕೙ߩ⺖㗴ߣ዁᧪㧦േ‛ᗲ⼔ᴺᡷᱜ໧㗴߳ߩ⵾⮎දߩขࠅ⚵ߺਛ᧛ ๺Ꮢ㧔ᣣᧄ⵾⮎Ꮏᬺදળ ක⮎ຠ⹏ଔᆔຬળ ၮ␆⎇ⓥㇱળ㧕 㧞㧚േ‛ታ㛎ߩ⥄ਥ▤ℂߩផㅴߦะߌߡ ́HS ⽷࿅ߩേ‛ታ㛎ᄖㇱ⹏ଔ࡮ᬌ⸽೙ᐲ́૒‫ ᧁޘ‬ᒎ↢㧔౏⋉⽷࿅ᴺੱ ࡅࡘ࡯ࡑࡦࠨࠗࠛࡦࠬᝄ⥝⽷࿅㧕㧟㧚Laboratory animal welfare might be resoluted by education and laws
PARK, J.H.㧔Seoul National University㧕㧨⛽ᜬળຬ࠾ࡘ࡯ࠬ㧪㧝㧚ᅚᕈഭ௛ၮḰⷙೣߩᡷᱜߣⅣႺᡷༀ⵾ຠ
⊕੗᧻ེ᪾㧔ᩣ㧕
㧞㧚㊀ᐲ఺∉ਇోേ‛㧔NSG ࡑ࠙ࠬ㧕ᣂⷙ࿖ౝ↢↥㐿ᆎ‫ޠ‬ᣣᧄ࠴ࡖ࡯࡞ࠬ࡮࡝ࡃ࡯㧔ᩣ㧕㧞㧕╙ 116 ࿁⎇ⓥળ㧔ᐔᚑ 24 ᐕ 12 ᦬ 14 ᣣ㧔㊄㧕‫ޔ‬⡛⼔㒮ᓮᲚ⨿㧕㧨ળຬߦࠃࠆ⊒⴫㧪 14 㗴㧨࠻ࡇ࠶ࠢࠬ㧪
Introduction of the Education Program for Animal Experiment in Seoul National
University
Jung Sun MIN, Byeong-Cheol KANG (Seoul National University)㧨․೎⻠Ṷ㧪
㧝㧚₞කᄖ⑼ቇ‫ޔ‬ታ㛎േ‛ቇ‫ޔ‬ታ㛎േ‛කቇ‫ޔ‬േ‛ታ㛎ઍᦧᴺቇ㤥Ỉ ദ㧔ᄢ㒋ᄢቇ කቇㇱ ታ㛎േ‛කቇᢎቶ㧕㧞㧚ታ㛎േ‛ߩ㗔ၞߦẜࠅㄟࠎߢᓧߚ߽ߩ⧔Ꮉ ᔘᄦ㧔੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳㧕㧟㧕╙ 117 ࿁⎇ⓥળ㧔ᐔᚑ 25 ᐕ 3 ᦬ 1 ᣣ㧔㊄㧕‫ޔ‬ᭉ෹ળ㙚㧕㧨⻠Ṷળ㧪 ࠹࡯ࡑ㧦ࠕࠞࡂ࡜䉟䊝䊥䈫䉷䊑䊤䊐䉞䉾䉲䊠䉕↪䈇䈩↢๮ේℂ䉕⸃䈐᣿䈎䈜㩷㧝㧚ࠕࠞࡂ࡜ࠗࡕ࡝ߩࡕ࠺࡞േ‛ൻߦะߌߚ⾗Ḯ࡮ᛛⴚ࡮ᖱႎၮ⋚ߩ⎇ⓥ㧦ᄖ்ᕈ∔ᖚߩᴦ≮ߣౣ↢⎇ⓥ߳ߩㆡ↪ࠍⷞ㊁ߦජ⪲ ⷫᢥ㧔╳ᵄᄢቇ ᄢቇ㒮↢๮ⅣႺ♽ ⣖␹⚻ᖱႎቇಽ㊁࡮ౣ↢↢ℂቇ⎇ⓥቶ㧕㧞㧚࠯ࡉ࡜ࡈࠖ࠶ࠪࡘߩ⦡⚛⚦⢩㑆⋧੕૞↪ߣࡄ࠲࡯ࡦᒻᚑᷰㆺ ᱜൎ㧔ᄢ㒋ᄢቇ ᄢቇ㒮↢๮ᯏ⢻⎇ⓥ⑼ ࡄ࠲࡯ࡦᒻᚑ⎇ⓥቶ㧕㧨࠻࠶ࡇࠢࠬ㧪ታ㛎↪ࠪࡠࡀ࠭ࡒߩ⿠Ḯᐶᧄ 㜞ᔒ㧔੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳㧕㧨⛽ᜬળຬ࠾ࡘ࡯ࠬ㧪㧝㧚㧔ᩣ㧕࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢΤᦼ᫟Ⓙ௛ߦߟ޿ߡ- นⷞൻࡑ࠙ࠬ‫⮎࡞ࠨޔ‬ℂ⹜㛎 ઁ㧔ᩣ㧕࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ
㧞㧚㔡ἴᤨߩ቟ో⏕଻ࠍ⋡⊛ߣߒߚታ㛎േ‛⎇ⓥᣉ⸳ߩ⠴㔡ኻ╷㧔᦭㧕࡚ࠠ࠙ࠛ࡯
㧠㧕╙ 118 ࿁⎇ⓥળ㧔ᐔᚑ 25 ᐕ 6 ᦬ 14 ᣣ㧔㊄㧕‫␹ޔ‬ᚭᄢቇකቇㇱળ㙚ࠪࠬࡔ࠶ࠢࠬࡎ࡯࡞㧕㧨⻠Ṷળ㧪 ࠹࡯ࡑ㧦ㆮવሶᡷᄌᛛⴚࠍ↪޿ߚ♧ዩ∛߅ࠃ߮േ⣂⎬ൻ⎇ⓥ
㧝㧚⢽⾰ઍ⻢࡮േ⣂⎬ൻ⎇ⓥߦ߅ߌࠆ࠙ࠨࠡߩ᦭↪ᕈ -apoA-II ࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ
࠙ࠨࠡߩ㐿⊒ߣᔕ↪ዊᳰ ᥓ਽㧔␹ᚭᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳㧕㧞㧚ᣂߚߥࠗࡦࠬ࡝ࡦಽᴲࡔࠞ࠾࠭ࡓߣ♧ዩ∛ᴦ≮⮎㐿⊒ߦ߅ߌࠆᗧ⟵      ᷡ㊁ ㅴ㧔␹ᚭᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ ಽሶઍ⻢කቇ㧕㧨࠻ࡇ࠶ࠢࠬ㧪㜞ᵴᕈဳ TALEN ߩ㐿⊒ߣ᭽‫ߥޘ‬േ‛ߦ߅ߌࠆࠥࡁࡓ✬㓸ጊᧄ ථ㧔ᐢፉᄢቇ ᄢቇ㒮ℂቇ⎇ⓥ⑼ ᢙℂಽሶ↢๮ℂቇኾ᡹㧕㧨⛽ᜬળຬ࠾ࡘ࡯ࠬ㧪ᒙ㉄ᕈᰴ੝Ⴎ⚛㉄᳓ṁᶧߩᵴ↪ߦߟ޿ߡ      㧔ᩣ㧕ࠛࠗ࠴࡮ࠛࠬ࡮ࡇ࡯
㧡㧕╙ 119 ࿁⎇ⓥળ㧔ᐔᚑ 25 ᐕ 9 ᦬ 13 ᣣ㧔㊄㧕‫ޔ‬ᄢ㒋ᄢቇ ㌁᧙ળ㙚㧕㧨⛽ᜬળຬ࠾ࡘ࡯ࠬ㧪
Zucker ࡜࠶࠻ࠃࠅ⷗಴ߐࠇߚᣂߒ޿∔∛ࡕ࠺࡞േ‛ߩ⚫੺ᣣᧄࠛࠬࠛ࡞ࠪ࡯㧔ᩣ㧕㧨⻠Ṷળ㧪 ࠹࡯ࡑ㧦↢૕ࠗࡔ࡯ࠫࡦࠣߩᦨ೨✢-఺∉ߩᣂߚߥ਎⇇㧝㧚↢߈ߚታ㛎േ‛ߢߩ↢૕ࠗࡔ࡯ࠫࡦࠣ㨪఺∉⚦⢩ߩേߊ਎⇇ߩ⸃ᨆ⍹੗ ఝ㧔ᄢ㒋ᄢቇ ᄢቇ㒮කቇ♽⎇ⓥ⑼ ఺∉⚦⢩↢‛ቇ㧕㧞㧚࡜ࠗࡉࠗࡔ࡯ࠫࡦࠣߦࠃࠅ᣿ࠄ߆ߦߥߞߚ⊹⤏఺∉ߩᣂ਎⇇ᬋፉ ஜᴦ㧔੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ ⊹⤏⑼ቇ㧕㧨࠻ࡇ࠶ࠢࠬ㧪
CRISPR/Cas9 ࠪࠬ࠹ࡓࠍ↪޿ߚࡑ࠙ࠬࠥࡁࡓ✬㓸દᎹ ᱜੱ㧔ᄢ㒋ᄢቇ ᓸ↢‛∛⎇ⓥᚲ ᗵᨴേ‛ታ㛎ᣉ⸳㧕ҋᐙ੐ળ‫⼏⹏ޔ‬ຬળ‫✚ޔ‬ળߩ⼏੐᭎ⷐҌᐙ੐ળߩ᭎ⷐ
ᣣᤨ㧦ᐔᚑ 25 ᐕ 2 ᦬ 8 ᣣ㧔㊄㧕‫ޔ‬ඦᓟ 4 ᤨ㨪႐ᚲ㧦੩ㇺᄢቇᄢቇ㒮කቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳ 4F ࠮ࡒ࠽࡯ቶ
಴Ꮸ⠪㧦㒙ㇱ‫ޔ‬㘵↰‫ޔ‬༑ᄙ‫ޔ‬ਭ଻‫ޔ‬ᐶᧄ‫ޔ‬᪀᧛‫ޔ‬ㄭ⮮‫ޔ‬Ⴎ⷗‫⧔ޔ‬Ꮉ‫↰ޔ‬ፉ‫ޔ‬ဝ↰‫ޔ‬ਛ੗ਛ᧛‫ޔ↰᧻ޔ‬ጊᷝ‫ޔ‬ᮮ੗‫ޔ‬ᳯධᰳᏨ⠪㧦ᳰ↰‫ޔ‬ጟ↰‫ޔ‬㤥Ỉ‫ޔ‬᫪ᧄ‫ޔ‬ጊᧄ⼏੐㧦䋱䋮㩷 ᐔᚑ 24 ᐕᐲ੐ᬺႎ๔
x⧔Ꮉળ㐳䉋䉍ᐔᚑ 24 ᐕᐲ੐ᬺႎ๔䈏䈅䈦䈢䇯䋲䋮㩷 ᐔᚑ 24 ᐕᐲ᳿▚ႎ๔xᐶᧄᐙ੐䉋䉍䇮ᐔᚑ 24 ᐕᐲ᳿▚ႎ๔䈏䈅䈦䈢䇯x⧔Ꮉળ㐳䉋䉍䇮ળ⾌ᧂ⚊⠪䈱ขᛒ䈇䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯䋳䋮㩷 ╙ 31 ࿁⹏⼏ຬળ䇮╙ 30 ࿁✚ળ䈱ઙx⹏⼏ຬળ䈱⼏㐳䈲䇮ળೣ䈮䉋䉍⧔Ꮉળ㐳䈏ᜂᒰ䈜䉎䈖䈫䉕⏕⹺䈚䈢䇯x✚ળ䈱ㅴⴕᓎ䈲䇮ᮮ੗ᐙ੐䈏ᜂᒰ䈜䉎䈖䈫䈫䈭䈦䈢䇯䋴䋮㩷 ᐔᚑ 25 ᐕᐲ੐ᬺ⸘↹᩺xx㒙ㇱᐙ੐䉋䉍䇮ᐔᚑ 25 ᐕᐲ੐ᬺ⸘↹᩺䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯ᐶᧄᐙ੐䉋䉍䇮╙ 117 ࿁⎇ⓥળ䋨ᐔᚑ 25 ᐕ 3 ᦬ 1 ᣣ䇮੩ㇺᄢቇ㩷 ᭉ෹ળ㙚䋩䈱㐿௅᭎ⷐ䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯xᮮ੗ᐙ੐䉋䉍䇮╙ 118 ࿁⎇ⓥળ䋨ᐔᚑ 25 ᐕ 6 ᦬ 14 ᣣ䇮␹ᚭᄢቇ㩷 䉲䉴䊜䉾䉪䊖䊷䊦䋩䈱㐿௅᭎ⷐ䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯x↰ፉᐙ੐䉋䉍䇮╙ 119 ࿁⎇ⓥળ䋨ᐔᚑ 25 ᐕ 9 ᦬ 6 ᣣ䇮ᄢ㒋ᄢቇ㩷 ㌁᧙ળ㙚䋩䈱㐿௅᭎ⷐ䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯x⧔Ꮉળ㐳䉋䉍䇮╙ 120 ࿁⎇ⓥળ䋨30 ๟ᐕ⸥ᔨᄢળ䇮ᐔᚑ 25 ᐕ 12 ᦬ 6 ᣣ䇮⡛⼔㒮ᓮᲚ⨿䋩䈱ડ↹䈮䈧䈇䈩䈲䇮ᐙ੐䈎䉌ᐢ䈒ᗧ⷗䉕൐䉎䉋䈉ଐ㗬䈏䈅䈦䈢䇯x⧔Ꮉળ㐳䉋䉍䇮ᯏ㑐⹹╙ 34 ภ䈪䈱⪭ৼ╬䈮䈧䈇䈩䇮⺑᣿䈏䈅䈦䈢䇯x

34 ภ䈱⸓ᱜ䈮䈧䈇䈩䈲䇮ᱜ⺋⴫䉕ળຬ䈮ㅍઃ䈜䉎䈖䈫䈫䈚䈢䇯x੹ᓟ䇮ේⓂ䈱ᩞ㑛䉕ⶄᢙੱ䈪ⴕ䈉䈖䈫䈫䈚䇮✬㓸ᜂᒰᐙ੐䈏䈖䉏䉕ᚲ᝿䈜䉎䈖䈫䈫䈭䈦䈢䇯䋵䋮㩷 ᐔᚑ 25 ᐕᐲ੍▚᩺xᐶᧄᐙ੐䉋䉍䇮ᐔᚑ 25 ᐕᐲ੍▚᩺䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯x30 ๟ᐕ⸥ᔨᄢળ䈫੐ോዪ䈱⒖ォ䉕⠨ᘦ䈚䈩䇮੍஻⾌䉕 10 ਁ౞䈫䈜䉎䈖䈫䈫䈚䈢䇯䋶䋮㩷 ੐ോዪ䈱⒖ォ䈮䈧䈇䈩x⧔Ꮉળ㐳䈎䉌䇮੐ോዪ䈱⒖ォ䈮䈧䈇䈩ឭ᩺䈏䈅䉍䇮ੌᛚ䈘䉏䈢䇯xળೣ䈱ᡷᱜ䉕䇮⹏⼏ຬળ䈮⻁䉎䈖䈫䈫䈭䈦䈢䇯䋷䋮㩷 ᣣᧄታ㛎േ‛ቇળቇⴚ㓸ળᆔຬળ䈎䉌䈱䉝䊮䉬䊷䊃䈮䈧䈇䈩x⧔Ꮉળ㐳䈎䉌䇮ᣣᧄታ㛎േ‛ቇળቇⴚ㓸ળᆔຬળਥ௅䈱䉲䊮䊘䉳䉡䊛䈱౒௅䈮㑐䈜䉎䉝䊮䉬䊷䊃䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯x⸛⼏䈱⚿ᨐ䇮㑐⷏ታ㛎േ‛⎇ⓥળ䈱࿁╵䈲䇮䉲䊮䊘䉳䉡䊛䈱౒௅䈪䈲䈭䈒䇮ᓟេ䈫䈜䉎䈖䈫䈫䈚䈢䇯䋸䋮㩷 䈠䈱ઁx༑ᄙᐙ੐䈎䉌䇮╙ 62 ࿁ታ㛎േ‛ቇળ✚ળ䉕ᐔᚑ 27 ᐕ 5 ᦬䈮੩ㇺ䈪ᄢળ㐳䈫䈚䈩㐿௅䈜䉎ᣦ䈱⺑᣿䈏䈅䉍䇮㑐⷏ታ㛎േ‛⎇ⓥળ䈻䈱දജ䈏᳞䉄䉌䉏䈢䇯ᣣᤨ㧦ᐔᚑ 25 ᐕ 9 ᦬ 30 ᣣ䋨㊄䋩䇮ඦᓟ 3 ᤨ䌾5 ᤨ႐ᚲ㧦੩ㇺᛛⴚ⑼ቇ䉶䊮䉺䊷 B ળ⼏ቶ಴Ꮸ⠪㧦㒙ㇱ䇮༑ᄙ䇮ਭ଻䇮ᐶᧄ䇮㤥Ỉ䇮᪀᧛䇮ㄭ⮮䇮Ⴎ⷗䇮⧔Ꮉ䇮↰ፉ䇮ဝ↰䇮ਛ੗䇮ਛ᧛䇮᧻↰䇮᫪ᧄ䇮ጊᷝ䇮ᮮ੗ᰳᏨ⠪㧦ᳰ↰䇮ጟ↰䇮ጊᧄ⼏੐㧦䋱䋮㩷 ╙ 120 ࿁⎇ⓥળ䋨30 ๟ᐕ⸥ᔨᄢળ䈮䈧䈇䈩䋩x༑ᄙᐙ੐䉋䉍╙ 120 ࿁⎇ⓥળ䈮䈧䈇䈩䊒䊨䉫䊤䊛䋨᩺䋩䈱⺑᣿䈏䈅䈦䈢䇯x㒙ㇱᐙ੐㐳䉋䉍䇮ㆊ෰䈱⸥ᔨᄢળ䈮㑐䈜䉎ㅊട⺑᣿䈏䈅䈦䈢䇯xඦ೨ਛ䈱䊒䊨䉫䊤䊛䋨㑐⷏ታ㛎േ‛⎇ⓥળ䈱ᱧผ䈫ᧂ᧪䋩䈮䇮৻㗴ㅊട䈜䉎䈖䈫䈫䈭䈦䈢䇯xᐳ㐳䈏᳿ቯ䈘䉏䈢䇯x੐ോዪ䉋䉍ળ႐䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯䋲䋮㩷 ╙ 11 ᦼ䈱ᓎຬㆬ಴䈮䈧䈇䈩x⧔Ꮉળ㐳䉋䉍䇮ᓎຬ䈱ᡷㆬ䈮䈧䈇䈩⺑᣿䈏䈅䈦䈢䇯x⧔Ꮉળ㐳䉋䉍䇮ળ㐳ㅌછ䈱ᗧะ䈏␜䈘䉏䈢䇯xᐙ੐䈱੕ㆬ䈮䉋䉍䇮༑ᄙᐙ੐䈏╙ 11 ᦼ䈱ળ㐳୥⵬䈫䈚䈩ㆬ಴䈘䉏䈢⹏⼏ຬળߩ᭎ⷐ
╙ 31 ࿁㑐⷏ታ㛎േ‛⎇ⓥળ⹏⼏ຬળᣣᤨ㧦ᐔᚑ 25 ᐕ 3 ᦬ 1 ᣣ㧔㊄㧕11:30㨪13:00

႐ᚲ㧦ᭉ෹ળ㙚 2 㓏⻠Ṷቶ಴Ꮸ㧦 43 ฬᵻ㊁⵨ਃ‫ޔ‬㒙ㇱᢅ↵‫ޔ‬ᳰ↰ථ਽‫ޔ‬ᳰ↰సᏆ‫੹ޔ‬੗⦟ᖝ‫ޔ‬਄↰ᱜᰴ‫ޔ‬ᄢ㊁᳃↢‫ޔ‬ጟ↰೑਽‫ޔ‬ጟᧄቬ⵨‫ޔ‬ᴒᧄ৻ᄦ‫ޔ‬ᤐᣣਭ↵‫ޔ‬༑ᄙᱜ๺‫ޔ‬ർ↰৻ඳ‫ޔ‬ਭ଻⮍‫ୖޔ‬ᨋ⼑‫ޔ‬ᐶᧄ㜞ᔒ‫ޔ‬㤥ᧁብੑ‫ޔ‬㤥Ỉദ‫ޔ‬᪀᧛ల‫ޔ‬ㄭ⮮㕏‫ޔ‬૒ട⦟⧷ᴦ‫ޔ‬Ⴎ⼱ᕶሶ‫ޔ‬㋈ᧁ᣹‫⧔ޔ‬Ꮉᔘᄦ‫ޔ‬㜞ᧁ⽵᣿‫ޔ‬㜞ፉବⴕ‫┻ޔ‬ਯਅ⺈‫↰ޔ‬ፉఝ‫ޔ‬ජ⪲⮍‫ޔ‬ဝ↰⵨ม‫ޔ‬ਛ੗િሶ‫ޔ‬ਛ᧛⚩৻㇢‫ޔ‬ᯅᧄᱜ᥍‫⌀ޔ‬ਅ⍮჻‫ޔ‬Ⴧጟㅢᄦ‫↰᧻ޔ‬Ả৻㇢‫ޔ‬ች᎒ብᓆ‫ޔ‬᫪ፉ⧷༑‫ޔ‬᫪ᧄ⚐ม‫ޔ‬ጊᷝ⵨ਯ‫ޔ‬ጊਛਭ‫ޔ‬ጊᧄᅢ↵‫ޔ‬ᮮ੗ષ⧷⼏੐㧦㧝㧚ᐔᚑ 24 ᐕᐲ੐ᬺႎ๔෸߮᳿▚ႎ๔x㒙ㇱᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 24 ᐕᐲߩ੐ᬺႎ๔߇޽ߞߚ‫ޕ‬xᐶᧄᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 24 ᐕᐲ෼ᡰ᳿▚ᦠߦߟ޿ߡႎ๔߇޽ߞߚ‫ޕ‬xጊᧄᐙ੐ࠃࠅ‫ޔ‬ળႎ 34 ภߦ߅ߌࠆ⪭ৼߣ⺋ࠅߦߟ޿ߡ⸓ᱜߣ߅⹟߮߇޽ߞߚ‫ޕ‬x

ᐔᚑ 24 ᐕᐲ෼ᡰ᳿▚ႎ๔߇ోળ৻⥌ߢᛚ⹺ߐࠇߚ‫ޕ‬㧞㧚ᐔᚑ 25 ᐕᐲ ੐ᬺ⸘↹㧔᩺㧕෸੍߮▚㧔᩺㧕x㒙ㇱᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 25 ᐕᐲߩ੐ᬺ⸘↹㧔᩺㧕߇⺑᣿ߐࠇߚ‫ޕ‬xᐶᧄᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 25 ᐕᐲ੍▚㧔᩺㧕ߦߟ޿ߡ⺑᣿߇޽ߞߚ‫ޕ‬x

ᐔᚑ 25 ᐲ੐ᬺ⸘↹㧔᩺㧕߅ࠃ੍߮▚㧔᩺㧕߇‫ోޔ‬ળ৻⥌ߢᛚ⹺ߐࠇߚ‫ޕ‬㧟㧚੹ᓟߩ੍ቯߦߟ޿ߡ
x㋈ᧁ⹏⼏ຬࠃࠅ‫ޔ‬12 ᦬ߩ⎇ⓥળߩᣣ⒟ߦߟ޿ߡ⾰໧߇޽ߞߚ‫ޕ‬x⧔Ꮉળ㐳ࠃࠅ‫ޔ‬ળ㐳ߩㇺว‫ޔ‬ળ႐ߩㇺวࠍ✚ว⊛ߦൊ᩺ߒ‫ޔ‬᳿ቯߒߡ޿ࠆߣߩ࿁╵߇޽ߞߚ‫ޕ‬x⧔Ꮉળ㐳ࠃࠅ‫ޔ‬㑐⷏ታ㛎േ‛⎇ⓥળߩ੹ᓟߩዷᦸߦߟ޿ߡᐢߊᗧ⷗ࠍ᳞߼ࠄࠇߚ‫ޕ‬୘‫ߩޘ‬ᗧ⷗ࠍࡔ࡯࡞ߢ໧߁ߎߣߣߒߚ‫ޕ‬㧠㧚੐ോዪߩ⒖ォߦߟ޿ߡx⧔Ꮉળ㐳ࠃࠅ‫⧔ޔ‬Ꮉߩቯᐕㅌ⡯ߦ઻޿‫ޔ‬੐ോዪࠍ੩ㇺᄢቇᄢቇ㒮කቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳߆ࠄ౏⋉⽷࿅ᴺੱ੩ㇺ⑼ቇᛛⴚ࠮ࡦ࠲࡯22 ภቶߦ⒖ォߔࠆߎߣ߇ឭ᩺
ߐࠇߚ‫ޕ‬xోળ৻⥌ߢᛚ⹺ߐࠇߚ‫ޕ‬

㧡㧚ߘߩઁ
x

༑ᄙᐙ੐ࠃࠅ‫ ╙ޔ‬62 ࿁ᣣᧄታ㛎േ‛ቇળ✚ળߩᄢળ㐳ࠍᜂᒰߔࠆߣߩႎ๔߇޽ߞ
ߚ‫ޕ‬xች᎒ฬ⹷ળຬࠃࠅ‫ޔ‬࿑ᦠ‫ޟ‬ᣣᧄታ㛎േ‛⎇ⓥળ߆ࠄᴺੱቇળ߳ߩ 12 ᐕ㑆‫੺⚫ߩޠ‬߇޽ߞߚ‫ޕ‬✚ળ䈱᭎ⷐ
╙ 30 ࿁㑐⷏ታ㛎േ‛⎇ⓥળ✚ળᣣᤨ㧦ᐔᚑ 25 ᐕ 3 ᦬ 1 ᣣ㧔㊄㧕13:00-13:30
႐ᚲ㧦ᭉ෹ળ㙚 2 㓏⻠Ṷቶมળߩᮮ੗ᐙ੐ࠃࠅ‫✚ޔ‬ળߩ㐿௅ߦ޽ߚࠅ⼏㐳ߩផ⮈߇᳞߼ࠄࠇߚ‫ޕ‬᪀᧛ળຬ߇⼏㐳ߦㆬ಴ߐࠇߚ‫ޕ‬⼏੐㧦㧝㧚ᐔᚑ 24 ᐕᐲ੐ᬺႎ๔෸߮᳿▚ႎ๔x㒙ㇱᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 24 ᐕᐲߩ੐ᬺႎ๔߇ߐࠇߚ‫ޕ‬xᐶᧄᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 24 ᐕᐲ᳿▚ႎ๔߇ߐࠇߚ‫ޕ‬xጊፒ⋙੐ࠃࠅ‫⋙ޔ‬ᩏࠍⴕߞߚ➅⿧㊄᳿▚ᦠߦߟ޿ߡႎ๔߇޽ߞߚ‫ޕ‬x

ᐔᚑ 24 ᐕᐲ੐ᬺႎ๔ߥࠄ߮ߦ᳿▚ႎ๔߇ోળ৻⥌ߢᛚ⹺ߐࠇߚ‫ޕ‬㧞㧚ᐔᚑ 25 ᐕᐲ੐ᬺ⸘↹෸੍߮▚᩺x㒙ㇱᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 25 ᐕᐲߩ੐ᬺ⸘↹㧔᩺㧕߇⺑᣿ߐࠇߚ‫ޕ‬xᐶᧄᐙ੐ࠃࠅ‫ޔ‬ᐔᚑ 25 ᐕᐲ੍▚㧔᩺㧕ߦߟ޿ߡ⺑᣿߇޽ߞߚ‫ޕ‬x

ᐔᚑ 24 ᐕᐲ੐ᬺ⸘↹㧔᩺㧕ߥࠄ߮ߦ੍▚㧔᩺㧕߇‫ోޔ‬ળ৻⥌ߢᛚ⹺ߐࠇߚ‫ޕ‬㧟㧚੐ോዪߩ⒖ォߦߟ޿ߡx⧔Ꮉળ㐳ࠃࠅ‫⧔ޔ‬Ꮉߩቯᐕㅌ⡯ߦ઻޿‫ޔ‬੐ോዪࠍ੩ㇺᄢቇᄢቇ㒮කቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳߆ࠄ౏⋉⽷࿅ᴺੱ੩ㇺ⑼ቇᛛⴚ࠮ࡦ࠲࡯22 ภቶߦ⒖ォߔࠆߎߣ߇ႎ๔
ߐࠇߚ‫߁઻ߦࠇߘޔߚ߹ޕ‬ળೣߩᄌᦝ߇␜ߐࠇߚ‫ޕ‬x

੐ോዪߩ⒖ォ‫ޔ‬ਗ߮ߦ‫ޔ‬ળೣߩᄌᦝ߇‫ోޔ‬ળ৻⥌ߢᛚ⹺ߐࠇߚ‫ޕ‬x㒙ㇱᐙ੐ࠃࠅ‫⛽ޔ‬ᜬળຬ࠾ࡘ࡯ࠬ߳ߩⓍᭂ⊛ߥෳടߩଐ㗬߇޽ߞߚ‫ޕ‬ҋળຬߩ⇣േҌ㧔ᐔᚑ 24 ᐕ 10 ᦬㨪ᐔᚑ 25 ᐕ 10 ᦬㧕
౉ળ⠪
ᦺᲧᄹ ⺈

ᱞ↰⮎ຠᎿᬺ (ᩣ) ၮ⋚ᛛⴚ⎇ⓥᚲ േ‛ࡕ࠺࡞ࠣ࡞࡯ࡊ

㣔㊁ ᱜ⥝␹ᚭቇ㒮ᄢቇ ⮎ቇㇱ ↢๮⮎ቇㇱ㐷 ⚦⢩↢‛ቇ⎇ⓥቶੑᎹ ᔀ(ᩣ) ࠗ࠽࡝ࠨ࡯࠴ ᄢ㒋ᡰᚲ᧲┵ ⵨ม⍹ේ↥ᬺ (ᩣ) ਛᄩ⎇ⓥᚲ ቟ో⑼ቇ⎇ⓥቶ ቟ోᕈࠣ࡞࡯ࡊㅌળ⠪ᷰㆺ ੳ⟤

੩ㇺᄢቇ ౣ↢ක⑼ቇ⎇ⓥᚲ 㒝ዻౣ↢ታ㛎േ‛ᣉ⸳ጊፒ ᮸㉿

ṑ⾐ක⑼ᄢቇ േ‛↢๮⑼ቇ⎇ⓥ࠮ࡦ࠲࡯㠽ข Ả৻(ᩣ) ࠫࡖࡄࡦࡈࠔ࡯ࡓ ࠢ࡜࠙ࡦ⎇ⓥᚲદ⮮ ෹৻ࠞࠗࠥࡦࡈࠔ࡯ࡑ (ᩣ)⮐ౝ ߆߅ࠅ(ᩣ) ᣂᣣᧄ⑼ቇ ቟ోᕈ⎇ⓥᚲ㘵↰ ᥏ᢅ㑐⷏ታ㛎േ‛⎇ⓥળ ୘ੱળຬฬ★㧔ᐔᚑ 25 ᐕ 10 ᦬⃻࿷㧕‫ڎ‬ળ㐳‫ޔ‬٤⹏⼏ຬ‫ޔ‬٧ᐙ੐‫⋙ٌޔ‬੐‫ڏޔ‬ฬ⹷ળຬ




᳁ฬ

ᚲዻ

޽

‫ޚ‬

ᵻ㊁ ⵨ਃ

(ᩣ) ࡏ࠱࡝ࠨ࡯࠴࠮ࡦ࠲࡯ ವධ⎇ⓥᚲᦺᲧᄹ ⺈ᱞ↰⮎ຠᎿᬺ (ᩣ) ၮ⋚ᛛⴚ⎇ⓥᚲ േ‛ࡕ࠺࡞ࠣ࡞࡯ࡊ᧲ ᢥ↵(ᩣ) ♿๺ታ㛎േ‛⎇ⓥᚲ

‫ޚ‬٧

቟୚ ብ᣿

ࡑ࡯ࠪࡖ࡞࡮ࡃࠗࠝ࡝࠰࡯ࠪࠬ࡮ࠫࡖࡄࡦ(ᩣ)቟ㇱ ᱞࠝ࡝ࠛࡦ࠲࡞㉂ᲣᎿᬺ (ᩣ)ᣂ੗ ஜผࠛ࡞ࠛࠬࠫ࡯ (ᩣ)᦭౷ ඳਯࠪࠝࡁࠡ⵾⮎ (ᩣ) ᣂ⮎⎇ ታ㛎േ‛▤ℂቶ቟⮮ ஜผࠝ࡝ࠛࡦ࠲࡞㉂ᲣᎿᬺ (ᩣ)᧘ ᚑ৻

㑐⷏ක⑼ᄢቇ 㒝ዻ↢๮කቇ⎇ⓥᚲ ࡕ࠺࡞േ‛ㇱ㐷ᳰ ㇚↢(⁛) ℂൻቇ⎇ⓥᚲ ࡃࠗࠝ࡝࠰࡯ࠬ࠮ࡦ࠲࡯

٤

ᳰ↰ సᏆ

ᱞᐶᎹᅚሶᄢቇ ⮎ቇㇱ

‫ޚ‬٧

ᳰ↰ ථ਽

ᣣᧄ࠴ࡖ࡯࡞ࠬ࡮࡝ࡃ࡯ (ᩣ)ᳰᷨ ৻਽ᄢ㢈⮎ຠᎿᬺ (ᩣ)੗Ỉ ᱞผᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ ₞ක∛ℂቇ⍹ဈ ๺ᒾ࠹ࠢ࠾ࡊ࡜ࠬ࠻࡮ࠫࡖࡄࡦ(ᩣ)෩ේ ⟤Ⓞ(ᩣ) ࡆࠝࠬ࠲દ⮮ 㓉ᐽᱞ↰⮎ຠᎿᬺ (ᩣ) ⮎೷቟ోᕈ⎇ⓥᚲੇ ବ⑲↰ㄝਃ⪉⵾⮎ (ᩣ) ⎇ⓥផㅴㇱੇ ౏ᱜ⍹ේ↥ᬺ (ᩣ) ਛᄩ⎇ⓥᚲੇ 㓷⤿

ᣣᧄක⑼ቇേ‛⾗᧚⎇ⓥᚲ੗਄ ീ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯੹੗ ⦟ᖝᱞ↰⮎ຠᎿᬺ (ᩣ) ḕධ⎇ⓥᚲ੹ᨋ Ả৻(ᩣ) DIMS ක⑼ቇ⎇ⓥᚲ

ᣂᲧᕺ ໪ᔒ

↰ㄝਃ⪉⵾⮎ (ᩣ) ᴺോㇱጤ⼱ ජ㢬ṑ⾐ක⑼ᄢቇ േ‛↢๮⑼ቇ⎇ⓥ࠮ࡦ࠲࡯਄↰ ᱜᰴ(ᩣ) ࡈࠚ࠾࠶ࠢࠬࡃࠗࠝ ቝㇺች੐ᬺᚲ਄㊁ ᷤ(⁛) ᡼኿✢කቇ✚ว⎇ⓥᚲ ታ㛎േ‛㐿⊒࡮▤ℂ⺖ౝየ ߎߕ߃

(⁛) ක⮎ၮ⋚⎇ⓥᚲ ∔ᖚࡕ࠺࡞ዊേ‛⎇ⓥቶᄗ㊁ ༀᒄᄢ㒋ᄢቇකቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳ᶏ㊁ 㓉(ᩣ) JCL ࡃࠗࠝࠕ࠶࠮ࠗ ⷏⣁࡜ࡏ

޿

٤

߁

٤

٤
߅

㒙ㇱ ᢅ↵

෸Ꮉ ᒄ

ᄢፉ ੖♿

Ⴎ㊁⟵⵾⮎ (ᩣ) ක⮎⎇ⓥ࠮ࡦ࠲࡯ᄢ↰ ⡛(ᩣ) ࠗࡉࡃࠗࠝࠨࠗࠛࡦࠬᄢ┻ ⡨(ᩣ) ࠝ࡝ࠛࡦ࠲࡞ࡃࠗࠝࠨ࡯ࡆࠬ ධጊၔ⎇ⓥᚲᄢဝ ⟵๺ᴛ੗⵾⮎ (ᩣ)ᄢ㊁ ᳃↢ฬฎደᄢቇ ᄢቇ㒮කቇ♽⎇ⓥ⑼ 㒝ዻකቇᢎ⢒ᡰេ࠮ࡦ࠲࡯ጟፒ ᓆ੫ࠛ࠺ࠬ࠻ࡠࡓࠫࡖࡄࡦ (ᩣ)

‫ޚ‬٧

ጟ↰ ೑਽

ᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ ታ㛎േ‛ቇ

٤

ጟᧄ ቬ⵨

੩ㇺᄢቇ㔤㐳㘃⎇ⓥᚲ ੱ㘃ㅴൻࡕ࠺࡞⎇ⓥ࠮ࡦ࠲࡯

⩆㊁ ାੑᄢᣣᧄ૑෹⵾⮎ (ᩣ) ⨙ᧁᎿ႐ᴒᧄ ৻ᄦ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ ࡃࠗࠝࠨࠗࠛࡦࠬ੐ᬺㇱ ∛ℂㇱ

㎛ጊ ჽ৻ᦶ

ᄢ㒋ᄢቇ කቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳ᤐᣣ ਭ↵(ᩣ) ᱞ↰࡜ࡆ࠶ࠢࠬട⮮ ໪ሶ੩ㇺ↥ᬺᄢቇ

㊄ሶ ᱞੱ੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳㏒ᧁ ജᷡ᳓ታ㛎᧚ᢱ(ᩣ)ᴡว Ẵሶᄢ㒋ᄢቇ කቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳ᴡ↰ ᤘᒾᣣᧄࠛࠬࠛ࡞ࠪ࡯ (ᩣ) ฃ⸤⎇ⓥㇱ␹↰ ᡽ౖႮ㊁⟵⵾⮎ (ᩣ) ᣂ⮎⎇ⓥᚲጯ ᚑᅢ(ᩣ) ࡟࠽࠹࠶ࠢ

‫ޚ‬٧

༑ᄙ ᱜ๺

੩ㇺᐭ┙ක⑼ᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ ታ㛎േ‛࠮ࡦ࠲࡯

٤

ർ↰ ৻ඳ

ർᶏ㆏ᄢቇ ᄢቇ㒮ℂቇ⎇ⓥ㒮ർ㊁ శᤘ(ᩣ) ࠞࡀࠞ ࡈࡠࡦ࠹ࠖࠕࡃࠗࠝ࡮ࡔ࠺ࠖࠞ࡞⎇ⓥᚲർ᧛ ౖ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯

٤
߆

٤

٤

߈



ᧁਅ ㇌᣿
ᧁ᧛ ࿖㓶
ߊ

ᣣᩉ ┨ᒾ

(ᩣ) ᣣᧄක⑼ቇേ‛⾗᧚⎇ⓥᚲਭ਎ ඳ(ᩣ) ࡏ࠱࡝ࠨ࡯࠴࠮ࡦ࠲࡯࿖෹ ৻ᦶࠛ࠼ࠬ࠻ࡠ࡯ࡓ (ᩣ)࿖ศ ାᕺ␹ᚭᄢቇ කቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳

‫ޚ‬٧

ਭ଻ ⮍

ᄹ⦟⋵┙ක⑼ᄢቇ వ┵කቇ⎇ⓥᯏ᭴ േ‛ታ㛎ᣉ⸳

٤

ୖᨋ ⼑

ጟጊᄢቇ කቇㇱ

‫ޚ‬٧

ᐶᧄ 㜞ᔒ

੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳

٤

㤥ᧁ ብੑ

ᄢᣣᧄ૑෹⵾⮎ (ᩣ)

‫ޚ‬٧

㤥Ỉ ദ

‫ޚ‬٧

᪀᧛ ల

ವධ⎇ⓥᚲ

ᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ ₞ක∛ℂቇ᪀᧛ ᦭ⷙ(ᩣ) ᣂᣣᧄ⑼ቇዊᳰ ᥓ਽␹ᚭᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳ዊᴰ ᷡ(ᩣ) DIMS ක⑼ቇ⎇ⓥᚲ

↲↰ ᓆࠛ࡞ࠛࠬࠫ࡯(ᩣ)ዊᶆ ⟤ᄹሶ

(⁛) ක⮎ၮ⋚⎇ⓥᚲ ∔ᖚࡕ࠺࡞ዊേ‛⎇ⓥቶዊ⼱ ␭ሶᄢ㒋ᄢቇ කቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳ฎ⮮ ᖳ੩ㇺ↥ᬺᄢቇዊᨋ ᰵṑ(ᩣ) ᣂᣣᧄ⑼ቇ 㣮ఽፉ∛ℂ࠮ࡦ࠲࡯ዊጊ ౏ᚑࠕࠬ࠹࡜ࠬ࡝ࠨ࡯࠴ࠨ࡯ࡆࠬ (ᩣ) ߟߊ߫⎇ⓥ࠮ࡦ࠲࡯

٤

ㄭ⮮ 㕏

↰ㄝਃ⪉⵾⮎ (ᩣ) ⋙ᩏㇱ

‫ޚ‬٧

ㄭ⮮ ₵

੩ㇺᄢቇ ౣ↢ක⑼ቇ⎇ⓥᚲ 㒝ዻౣ↢ታ㛎േ‛ᣉ⸳ㄭ⮮ ෹ብᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ ታ㛎േ‛ቇ

੹㊁ ౗ᰴ㇢

੩ㇺ↥ᬺᄢቇ㥲⮮ ᶈలਃ㊀ᄢቇ කቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳ਃᨑ 㗅ਃᣣᧄታ㛎േ‛ቇળ ੐ോዪဈ↰ ᄥੑ(ᩣ) ↰ㄝ㧾㧒㧰ࠨ࡯ࡆࠬ ടፉ ታ㛎േ‛▤ℂㇱ

ဈ↰ ㅴ

⇰ᄩᄢቇ

ဈᧄ 㓶ੑ

ජኼ⵾⮎ (ᩣ)૒ട⦟ ⧷ᴦ

౓ᐶක⑼ᄢቇ േ‛ታ㛎ᣉ⸳૒‫ ᧁޘ‬᣽ᔒᄢᣣᧄ૑෹⵾⮎ (ᩣ) ⎇ⓥ▤ℂㇱ૒⮮ ᶈ⥄ὼ⑼ቇ⎇ⓥᯏ᭴ ↢ℂቇ⎇ⓥᚲ

ߎ

٤

ߐ

٤

٤

૒⮮ ⑲⬿
㞯ፉ ⑲ᥰ

ߒ

Ỉᶆ 㓷ੱ

ᣣᧄ࠴ࡖ࡯࡞ࠬ࡮࡝ࡃ࡯ (ᩣ) ࠞࠬ࠲ࡑ࡯ࠨࡐ࡯࠻࠮ࡦ࠲࡯

‫ޚ‬٧

Ⴎ⷗ 㓷ᔒ

␹ᚭᄢቇᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳

٤

Ⴎ⼱ ᕶሶ

࿖┙ᓴⅣེ∛࠮ࡦ࠲࡯⎇ⓥᚲ േ‛▤ℂቶ

ٌ

ᷡ᳓ ⧷↵

ᷡ᳓ታ㛎᧚ᢱ (ᩣ)ᷡ᳓ ᄢ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ᷡ᳓ ૗৻ᷡ᳓ታ㛎᧚ᢱ (ᩣ)⊕⍹ ᒄਯ

ࡑ࡞ࡎ (ᩣ) ੩ㇺ R&D ࠮ࡦ࠲࡯ ක⮎㐿⊒⎇ⓥᚲ

ߔ

᧖੗ ቇ

٤

᧖ᯅ ⵨㆏

(ᩣ) ࠝ࡝ࠛࡦ࠲࡞ࡃࠗࠝࠨ࡯ࡆࠬ ␹ᚭ BM ࡜ࡏ࡜࠻࡝࡯

㋈ᧁ ᣹

ਃ㊀ᄢቇ ↢๮⑼ቇ⎇ⓥᡰេ࠮ࡦ࠲࡯ ᯏ⢻ࠥࡁࡒࠢࠬಽ㊁㋈ᧁ ⒤Ⴎ㊁⟵⵾⮎(ᩣ) ഃ⮎࡮ត⚝⎇ⓥᚲ㗇⮮ ᦭ੑࠕࠬ࠹࡜ࠬ࡝ࠨ࡯࠴࠹ࠢࡁࡠࠫ࡯(ᩣ)

ߖ

٤‫ڎ‬

⧔Ꮉ ᔘᄦ

੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ / ੩ㇺ∔ᖚࡕ࠺࡞⎇ⓥᚲᦥᚒ ᱜᒾႮ㊁⟵⵾⮎ (ᩣ) ᣂ⮎⎇ⓥᚲ㜞ᧁ ⽵᣿ᣣᧄࠛࠬ࡮ࠛ࡞࡮ࠪ࡯ (ᩣ)

㜞ᧁ ᑿᨑᣣᧄࠛࠬ࡮ࠛ࡞࡮ࠪ࡯ (ᩣ)

㜞ᧁ ᐽඳ(ᩣ㧕ᩕ᳓ൻቇ㜞ፉ ବⴕࡂࡓ࡝࡯㧔ᩣ㧕࿖㓙੐ᬺㇱ ᄢ㒋಴ᒛᚲ㣔㊁ ᱜ⥝␹ᚭቇ㒮ᄢቇ ⮎ቇㇱ ↢๮⮎ቇㇱ㐷 ⚦⢩↢‛ቇ⎇ⓥቶ㜞ᯅ ዏ჻(ᩣ) ࡆࠝࠬ࠲┻㢬 ⵨੫੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳

٤

┻ਯਅ ⺈

(ᩣ) ࠗࡉࡃࠗࠝࠨࠗࠛࡦࠬ

‫ޚ‬٧

↰ፉ ఝ

ᄢ㒋ᄢቇ කቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳↰ፉ ᦮ሶᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼↰ਛ ථੑ(ᩣ) ᧲ᶏ⚦⢩⎇ⓥᚲ↰ਛ ᓆੱ੩ㇺᄢቇ iPS ⚦⢩⎇ⓥᚲ᫜ᯅ ෹ሶ(ᩣ) ࠝ࡝ࠛࡦ࠲࡞ࡃࠗࠝࠨ࡯ࡆࠬ ༡ᬺㇱᄙᩮ੗ ᣽ቁ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ ༡ᬺᧄㇱජ⪲ ⮍(ᩣ) JT ࠢ࡝ࠛࠗ࠹ࠗࡉࠨ࡯ࡆࠬ 㜞ᮎ੐ᬺᚲ േ‛▤ℂ⺖Ⴆේ ᷡᔒࠪࠝࡁࠡ࠹ࠢࡁࠕ࠼ࡃࡦࠬ࡝ࠨ࡯࠴ (ᩣ)

ㄞ ཅᤘࠨࡦ࠲ࠨ࡯ࡠ㧒ࠬ࠹࡝-ࡊࡠ ࠰࡝ࡘ࡯࡚ࠪࡦ(ᩣ)

ㄞ ᱜ⛮(ᩣ) ࠫࠚ࡯ࠛ࡯ࠪ࡯ဝ↰ ⵨มᄢ㒋ᴡ㦮࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦᄢቇ ࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦቇㇱ㢬↰ ᕺਃ(ᩣ) ᣂᣣᧄ⑼ቇ಴ญ ᄩ჻੩ㇺᄢቇ ౣ↢ක⑼ቇ⎇ⓥᚲ 㒝ዻౣ↢ታ㛎േ‛ᣉ⸳ኹ᎑ ᤘᄦ⑼⎇⵾⮎(ᩣ) ✚ว⎇ⓥᚲ࿯੗ ᷡᒄ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯㠽ዬ ࿖᡽(ᩣ) ࠫࠚ࡯ࠛ࡯ࠪ࡯

⋥੗ ࿖ሶ㊄ᴛᄢቇ ߇ࠎㅴዷ೙ᓮ⎇ⓥᚲਛ੗ િሶᣣᧄᣂ⮎ (ᩣ) ⎇㐿ડ↹⛔᜝ㇱ⎇㐿▤ℂ⺖ਛ੗ ஜผႮ㊁⟵⵾⮎ (ᩣ) ታ㛎േ‛⎇ⓥ࠮ࡦ࠲࡯ਛ⷏ ⡡੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳ਛේ ᗲ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ਛ᧛ ⚩৻ᦶ

ṑ⾐ක⑼ᄢቇ േ‛↢๮⑼ቇ⎇ⓥ࠮ࡦ࠲࡯ਛ᧛ ᱜౖࠞ࡞࠽ࡃࠗࠝࠨࠗࠛࡦࠬ (ᩣ) ⎇ⓥ㐿⊒ㇱ

ߘ
ߚ

٤

٤

ߜ

٤

ߟ

‫ޚ‬٧

ߡ

ߣ

ߥ
‫ޚ‬٧

‫ޚ‬٧

ਛጊ ੫
ᄐ⋡ సᒾ

ᄐ⋡⵾૞ᚲ (ᩣ)

ߦ

ਗᴡ ⍮ሶ

ᴛ੗⵾⮎ (ᩣ) ↢‛⎇ⓥㇱ⷏੗ ᐽᕺᐞᄩᄢቇ ஜᐽ⑼ቇㇱ⷏Ꮉ ື੩ㇺ⮎⑼ᄢቇ േ‛⎇ⓥ࠮ࡦ࠲࡯

⷏᧛ ᱜᒾ
⷏᧛ ᒄ㆏

(ᩣ) ࠛ㧙࠹࠶ࠢ⷏ጊ ℭ⟤ᱞᐶᎹᅚሶᄢቇ ⮎ቇㇱ⷏ጊ ⑲ᔒ(ᩣ) ᱞ↰࡜ࡆ࠶ࠢࠬᣂ↰ 㕒㚅ࠪࠝࡁࠡ࠹ࠢࡁࠕ࠼ࡃࡦࠬ࡝ࠨ࡯࠴ (ᩣ)

ߨ

ᩮᧄ ⦟ᄦ

ࠪࠝࡁࠡ࠹ࠢࡁࠕ࠼ࡃࡦࠬ࡝ࠨ࡯࠴ (ᩣ)ߩ

㊁‫ޘ‬ญ ๺ᐘ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ᯅᧄ ᱜ᥍(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ ࠕ࠾ࡑ࡞ࠤࠕ੐ᬺᧄㇱᵿ੗ ⟤ᒎ⥄ὼ⑼ቇ⎇ⓥᯏ᭴ ↢ℂቇ⎇ⓥᚲ NBR ੐ᬺផㅴቶᵿ↰ ୃ৻ਃ⪉ൻቇ቟ో⑼ቇ⎇ⓥᚲ 㣮ፉ⎇ⓥᚲ Ქᕈ╙ 2 ࠣ࡞࡯ࡊේญ ᔃ㓶Ⴎ㊁⟵⵾⮎ (ᩣ) ක⮎⎇ⓥ࠮ࡦ࠲࡯ේ↰ ᱜผᄢ㒋Ꮢ┙ᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳᧲┵ ⵨ม⍹ේ↥ᬺ (ᩣ) ਛᄩ⎇ⓥᚲ ቟ో⑼ቇ⎇ⓥቶ ቟ోᕈࠣ࡞࡯ࡊᒁ࿯ ⍮ᐘᄢᣣᧄ㒰⯻⩵ (ᩣ) ਛᄩ⎇ⓥᚲ㘧↰ ᐽቁ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯

٤

ᐔᎹ ౏ᤘ

(ᩣ) ᣂᣣᧄ⑼ቇ ቟ోᕈ⎇ⓥᚲ ∛ℂ࠮ࡦ࠲࡯

٤

ᐔᴛ ീ

ࠪࠝࡁࠡ࠹ࠢࡁࠕ࠼ࡃࡦࠬ࡝ࠨ࡯࠴ (ᩣ)ᐔ᎑ มᄢႦ⵾⮎ (ᩣ) ᓼፉ⎇ⓥᚲᑝἑ ᷡ㚅

Ⴎ㊁⟵⵾⮎ (ᩣ) ᴤᣣ࡜ࡏ࡜࠻࡝࡯࠭ᑝ↰ ᥦᄹ੩ㇺ↥ᬺᄢቇ⑔ጟ ବᢥᄢᣣᧄ૑෹⵾⮎ (ᩣ) ⎇ⓥ▤ℂㇱ⮮ᴛ ౏ᔘᣣᧄ࠴ࡖ࡯࡞ࠬ࡮࡝ࡃ࡯ (ᩣ) ࠞࠬ࠲ࡑ࡯ࠨࡐ࡯࠻࠮ࡦ࠲࡯⮮↰ ๺㓉ࠪࠝࡁࠡ࠹ࠢࡁࠕ࠼ࡃࡦࠬ࡝ࠨ࡯࠴ (ᩣ)ੑᎹ ᔀ(ᩣ㧕ࠗ࠽࡝ࠨ࡯࠴ ᄢ㒋ᡰᚲ㣽 ⋥ᶈධጟጊක≮࠮ࡦ࠲࡯ ␹⚻ౝ⑼ᤊ ାᒾ␹ᚭᄢቇᄢቇ㒮ㄘቇ⎇ⓥ⑼ ᔕ↪േ‛ቇᤊ㊁ 㓷ⴕ(ᩣ) ᤊ㊁⹜㛎േ‛㘺⢒ᚲၳ ቁม(ᩣ) ࠝ࡝ࠛࡦ࠲࡞ࡃࠗࠝࠨ࡯ࡆࠬၳᳯ ା৻(ᩣ) ࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ೨Ꮉ ᥓ᮸ฬฎደᄢቇ ᄢቇ㒮කቇ♽⎇ⓥ⑼ ታ㛎േ‛⑼ቇ

ߪ

٤

٤
߭

߰

߶

٤

߹

೨↰ ᢅብ
٤

⌀ਅ ⍮჻

੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳

٤

Ⴧጟ ㅢᄦ

(ᩣ) ࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ‫ޚ‬٧᧻↰ Ả৻㇢

(⁛) ක⮎ၮ⋚⎇ⓥᚲ ∔ᖚࡕ࠺࡞ዊേ‛⎇ⓥቶ᧻ᧄ ⠹ਃ੩ㇺ↥ᬺᄢቇ Ꮏቇㇱ ↢‛Ꮏቇ⑼᳓ౝ ඳ↰ㄝਃ⪉⵾⮎ (ᩣ) ⮎‛േᘒ⎇ⓥᚲ᳓㊁ ା຦ᄢ㒋ᄢቇ ᄢቇ㒮කቇ♽⎇ⓥ⑼ ಽሶౣ↢කቇ᳓㊁ ᵗሶᄢ㒋ᄢቇ Ⱞ⊕⾰⎇ⓥᚲ 8 F േ‛ቶ

٤

ችਅ ାᴰ

㚅Ꮉᄢቇ ⎇ⓥផㅴᯏ᭴ ✚ว↢๮⑼ቇ⎇ⓥ࠮ࡦ࠲࡯ േ‛ታ㛎ㇱ㐷

٤‫ڏ‬

ች᎒ ብᓆ

(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯

٤

ች᎑ ᱜᐽ

๺᱌ጊ⋵┙ක⑼ᄢቇ ታ㛎േ‛ᣉ⸳ች࿾ ဋ੩ㇺᄢቇ ࠙ࠗ࡞ࠬ⎇ⓥᚲ 㒝ዻᗵᨴ∝ࡕ࠺࡞࠮ࡦ࠲࡯

ߺ

ችᧄ ⺈
ች⣁ ⨃᮸
߽

᫪ ᐙ㓶

ᄢᣣᧄ૑෹⵾⮎ (ᩣ) ⎇ⓥ▤ℂㇱ ▤ℂ╙ 2 㩂㩨㩣㨺㩖㩩

᫪ጟ ብ⥋
᫪ጟ ৻ノ

ࡠ࡯࠻⵾⮎ (ᩣ) ↢‛⥃ᐥ⎇ⓥㇱ 㐿⊒ᡰេ G

٤

᫪ፉ ⧷༑

ᱞ↰⮎ຠᎿᬺ (ᩣ) ⮎೷቟ోᕈ⎇ⓥᚲ

‫ޚ‬٧

᫪ᧄ ⚐ม

ᄢ㒋ක⑼ᄢቇᄢቇ㒮කቇ⎇ⓥ⑼⎇ⓥᯏ᭴ታ㛎േ‛࠮ࡦ࠲࡯቟႐ ᱜሶ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ ࡃࠗࠝࠨࠗࠛࡦࠬ੐ᬺㇱ⍫㊁ ⧷᮸(ᩣ) ࠝ࡝ࠛࡦ࠲࡞ࡃࠗࠝࠨ࡯ࡆࠬጊญ ୃ(ᩣ) ᄢ㒋ࡆ࡞▤ℂ േ‛▤ℂㇱ

ٌ

ጊ㦮 ┨ᒄ

ࠝ࡝ࠛࡦ࠲࡞㉂ᲣᎿᬺ㧔ᩣ㧕᧲ᣣᧄࡃࠗࠝ༡ᬺㇱ

‫ޚ‬٧

ጊᷝ ⵨ਯ

૑෹ൻቇ (ᩣ) ↢‛ⅣႺ⑼ቇ⎇ⓥᚲ

٤

ጊ↰ ቱ᳗

ᣂẟᄢቇㄘቇㇱ ⾗Ḯേ‛ㆮવቇಽ㊁ጊ↰ ◊ࠕࠬ࠹࡜ࠬ࡝ࠨ࡯࠴࠹ࠢࡁࡠࠫ࡯ (ᩣ) ቟ోᕈ⎇ⓥㇱጊ↰ ᵏᐢ੩ㇺᄢቇ iPS ⚦⢩⎇ⓥᚲጊᚻ ਂ⥋ᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ ₞ක∛ℂቇ

٤

ጊਛ ਭ

(ᩣ) ࠗ ࠽࡝ࠨ࡯࠴

‫ޚ‬٧

ጊᧄ ᅢ↵

ਃ㊀ᄢቇ ␠ળㅪ៤⎇ⓥ࠮ࡦ࠲࡯ દ⾐⎇ⓥ᜚ὐ

٤

ጊᧄ ඳ

ንጊᄢቇ ↢๮⑼ቇవ┵⎇ⓥ࠮ࡦ࠲࡯ േ‛⾗Ḯ㐿⊒ಽ㊁ጊᧄ ඳ┨㐳ᵿࡃࠗࠝᄢቇ ࡃࠗࠝࠨࠗࠛࡦࠬቇㇱ࡙࠙ ࠗࡦ␹ᚭᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳

߿

ࠁ
ࠃ

‫ޚ‬٧

ᮮ੗ ષ⧷

␹ᚭᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ ಽሶઍ⻢කቇ

ࠊ



⧯⁜ ⧐↵

(ᩣ) ࠗ࠽࡝ࠨ࡯࠴ ᧲੩ᡰᚲ㑐⷏ታ㛎േ‛⎇ⓥળ ⛽ᜬળຬฬ★ 㧔ᐔᚑ 25 ᐕᐲ㧕㧔޽޿߁߃߅㗅㧕ࠕࠬ࠹࡜ࠬ࡝ࠨ࡯࠴࠹ࠢࡁࡠࠫ࡯ᩣᑼળ␠ േ‛▤ℂㇱ
ࠛ࡞ࠛࠬࠫ࡯ᩣᑼળ␠
ࠝ࡝ࠛࡦ࠲࡞㉂ᲣᎿᬺᩣᑼળ␠ ᄢ㒋ࡃࠗࠝ༡ᬺᚲ
ᩣᑼળ␠ࠕࠗ࠮ࠗ
ᩣᑼળ␠ࠗ࠽࡝ࠨ࡯࠴
ᩣᑼળ␠ࠗࡉࡃࠗࠝࠨࠗࠛࡦࠬ
ᩣᑼળ␠ࠛࠗ࠴࡮ࠛࠬ࡮ࡇ࡯
ᩣᑼળ␠ࠛ࡯࠹࠶ࠢ
ᩣᑼળ␠ᄢႦ⵾⮎Ꮏ႐ ⎇ⓥ㐿⊒࠮ࡦ࠲࡯ᩣᑼળ␠ࠝ࡝ࠛࡦ࠲࡞ࡃࠗࠝࠨ࡯ࡆࠬ
ᩣᑼળ␠ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯
ᩣᑼળ␠ࠫࡖࡄࡦࡈࠔ࡯ࡓ ࠢ࡜࠙ࡦ⎇ⓥᚲ
ᩣᑼળ␠ᣂᣣᧄ⑼ቇ ᄢ㒋ᡰ␠ᩣᑼળ␠♖⎇
ᩣᑼળ␠࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ
ᩣᑼળ␠ᄐ⋡⵾૞ᚲᩣᑼળ␠ᣣᧄක⑼ቇേ‛⾗᧚⎇ⓥᚲ
ᩣᑼળ␠ࡆࠝࠬ࠲
ᩣᑼળ␠ࡈࠚ࠾࠶ࠢࠬࡃࠗࠝ
ᩣᑼળ␠⟤Ớ࡜ࡏᩣᑼળ␠ࡔ࠺ࠖࠛ࡯࠻
ᩣᑼળ␠࡟࠽࠹࠶ࠢ
ർጊ࡜ߴࠬᩣᑼળ␠
⽷࿅ᴺੱേ‛❥ᱺ⎇ⓥᚲ
ਃද࡜ࡏࠨ࡯ࡆࠬᩣᑼળ␠
ෳᄤ⵾⮎ᩣᑼળ␠ ⎇ⓥ㐿⊒࠮ࡦ࠲࡯ࠪࠝࡁࠡ࠹ࠢࡁࠕ࠼ࡃࡦࠬ࡝ࠨ࡯࠴ᩣᑼળ␠
ᷡ᳓ታ㛎᧚ᢱᩣᑼળ␠
⊕੗᧻ེ᪾ᩣᑼળ␠
ᄢᣣᧄ૑෹⵾⮎ᩣᑼળ␠ ⎇ⓥ▤ℂㇱ ▤ℂࠣ࡞࡯ࡊ㜞Ⴆ࡜ࠗࡈࠨࠗࠛࡦࠬᩣᑼળ␠
ᣣ♖ࡃࠗ࡝ࠬᩣᑼળ␠
ᣣᧄࠛࠬࠛ࡞ࠪ࡯ᩣᑼળ␠
ᣣᧄࠢ࡟ࠕᩣᑼળ␠ ᄢ㒋੐ᬺᚲᣣᧄᣂ⮎ᩣᑼળ␠ ഃ⮎⎇ⓥᚲ
ᣣᧄ࠴ࡖ࡯࡞ࠬ࡮࡝ࡃ࡯ᩣᑼળ␠
ࡂࡓ࡝࡯ᩣᑼળ␠ ᄢ㒋༡ᬺᚲਣ⍹⵾⮎ᩣᑼળ␠ ਛᄩ⎇ⓥᚲ
ਃᶆᎿᬺᩣᑼળ␠ ࡔ࠺ࠗࠞ࡞⷏ᣣᧄ༡ᬺㇱ᦭㒢ળ␠࡚ࠠ࠙ࠛ࡯
᦭㒢ળ␠ࡔ࠺ࠖࠕ

㑐⷏ታ㛎േ‛⎇ⓥળ ⹏⼏ຬฬ★㧔ᐔᚑ 23-25 ᐕᐲ㧕
᳁ฬ

ᚲዻ

ᵻ㊁ ⵨ਃ

(ᩣ) ࡏ࠱࡝ࠨ࡯࠴࠮ࡦ࠲࡯ ವධ⎇ⓥᚲ

㒙ㇱ ᢅ↵
ᳰ↰ ථ਽

ᣣᧄ࠴ࡖ࡯࡞ࠬ࡮࡝ࡃ࡯ (ᩣ)ᳰ↰ సᏆᱞᐶᎹᅚሶᄢቇ ⮎ቇㇱ੹੗ ⦟ᖝᱞ↰⮎ຠᎿᬺ (ᩣ) ḕධ⎇ⓥᚲ਄↰ ᱜᰴ(ᩣ) ࡈࠚ࠾࠶ࠢࠬࡃࠗࠝ ቝㇺች੐ᬺᚲᶏ㊁ 㓉(ᩣ) JCL ࡃࠗࠝࠕ࠶࠮ࠗ

ᄢ㊁ ᳃↢ฬฎደᄢቇ ᄢቇ㒮කቇ♽⎇ⓥ⑼ 㒝ዻකቇᢎ⢒ᡰេ࠮ࡦ࠲࡯ጟ↰ ೑਽ᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ ታ㛎േ‛ቇጟᧄ ቬ⵨੩ㇺᄢቇ㔤㐳㘃⎇ⓥᚲ ੱ㘃ㅴൻࡕ࠺࡞⎇ⓥ࠮ࡦ࠲࡯ᴒᧄ ৻ᄦ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ ࡃࠗࠝࠨࠗࠛࡦࠬ੐ᬺㇱ ∛ℂㇱᤐᣣ ਭ↵(ᩣ) ᱞ↰࡜ࡆ࠶ࠢࠬ༑ᄙ ᱜ๺੩ㇺᐭ┙ක⑼ᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ ታ㛎േ‛࠮ࡦ࠲࡯ർ↰ ৻ඳർᶏ㆏ᄢቇ ᄢቇ㒮ℂቇ⎇ⓥ㒮ਭ଻ ⮍ᄹ⦟⋵┙ක⑼ᄢቇ వ┵කቇ⎇ⓥᯏ᭴ േ‛ታ㛎ᣉ⸳ୖᨋ ⼑ጟጊᄢቇ කቇㇱᐶᧄ 㜞ᔒ੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳㤥ᧁ ብੑᄢᣣᧄ૑෹⵾⮎ (ᩣ)

㤥Ỉ ദ
᪀᧛ ల

ᄢ㒋ᐭ┙ᄢቇ ᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ ₞ක∛ℂቇዊᨋ ᰵṑ(ᩣ) ᣂᣣᧄ⑼ቇ 㣮ఽፉ∛ℂ࠮ࡦ࠲࡯ㄭ⮮ ₵੩ㇺᄢቇ ౣ↢ක⑼ቇ⎇ⓥᚲㄭ⮮ 㕏↰ㄝਃ⪉⵾⮎ (ᩣ)૒ട⦟ ⧷ᴦ

౓ᐶක⑼ᄢቇ േ‛ታ㛎ᣉ⸳૒⮮ ᶈ⥄ὼ⑼ቇ⎇ⓥᯏ᭴ ↢ℂቇ⎇ⓥᚲႮ⷗ 㓷ᔒ␹ᚭᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳Ⴎ⼱ ᕶሶ࿖┙ᓴⅣེ∛࠮ࡦ࠲࡯⎇ⓥᚲ േ‛▤ℂቶ

㋈ᧁ ᣹ਃ㊀ᄢቇ ↢๮⑼ቇ⎇ⓥᡰេ࠮ࡦ࠲࡯⧔Ꮉ ᔘᄦ੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ / ੩ㇺ∔ᖚࡕ࠺࡞⎇ⓥᚲ㜞ᧁ ⽵᣿ᣣᧄࠛࠬ࡮ࠛ࡞࡮ࠪ࡯ (ᩣ)

㜞ፉ ବⴕࡂࡓ࡝࡯ (ᩣ) ࿖㓙੐ᬺᚲ ᄢ㒋಴ᒛᚲ┻ਯਅ ⺈(ᩣ) ࠗࡉࡃࠗࠝࠨࠗࠛࡦࠬ↰ፉ ఝᄢ㒋ᄢቇ කቇㇱ 㒝ዻേ‛ታ㛎ᣉ⸳ජ⪲ ⮍(ᩣ) JT ࠢ࡝ࠛࠗ࠹ࠗࡉࠨ࡯ࡆࠬ 㜞ᮎ੐ᬺᚲ േ‛▤ℂ⺖ဝ↰ ⵨มᄢ㒋ᴡ㦮࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦᄢቇ ࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦቇㇱਛ੗ િሶᣣᧄᣂ⮎ (ᩣ) ⎇㐿ડ↹⛔᜝ㇱਛ᧛ ⚩৻ᦶ

ṑ⾐ක⑼ᄢቇ േ‛↢๮⑼ቇ⎇ⓥ࠮ࡦ࠲࡯ᯅᧄ ᱜ᥍(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ ࠕ࠾ࡑ࡞ࠤࠕ੐ᬺᧄㇱේ↰ ᱜผᄢ㒋Ꮢ┙ᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ േ‛ታ㛎ᣉ⸳ᐔᎹ ౏ᤘ(ᩣ) ᣂᣣᧄ⑼ቇ ᄢ㒋∛ℂ࠮ࡦ࠲࡯ᐔᴛ ീࠪࠝࡁࠡ࠹ࠢࡁࠕ࠼ࡃࡦࠬ࡝ࠨ࡯࠴ (ᩣ)ᤊ ାᒾ␹ᚭᄢቇ ᄢቇ㒮ㄘቇ⎇ⓥ⑼ ᔕ↪േ‛ቇ⌀ਅ ⍮჻੩ㇺᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ 㒝ዻേ‛ታ㛎ᣉ⸳Ⴧጟ ㅢᄦ(ᩣ) ࠻࡜ࡦࠬࠫࠚ࠾࠶ࠢ᧻↰ Ả৻㇢

(⁛) ක⮎ၮ⋚⎇ⓥᚲ ∔ᖚࡕ࠺࡞ዊേ‛⎇ⓥቶችਅ ାᴰ㚅Ꮉᄢቇ ⎇ⓥផㅴᯏ᭴ ✚ว↢๮⑼ቇ⎇ⓥ࠮ࡦ࠲࡯ േ‛ታ㛎ㇱ㐷ች᎒ ብᓆ(ᩣ) ࠤ࡯࡮ࠛ࡯࡮ࠪ࡯ች᎑ ᱜᐽ๺᱌ጊ⋵┙ක⑼ᄢቇ ታ㛎േ‛ᣉ⸳᫪ፉ ⧷༑ᱞ↰⮎ຠᎿᬺ (ᩣ) ⮎೷቟ోᕈ⎇ⓥᚲ᫪ᧄ ⚐มᄢ㒋ක⑼ᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ ⎇ⓥᯏ᭴ ታ㛎േ‛࠮ࡦ࠲࡯ጊᷝ ⵨ਯ૑෹ൻቇ (ᩣ) ↢‛ⅣႺ⑼ቇ⎇ⓥᚲጊ↰ ቱ᳗ᣂẟᄢቇㄘቇㇱ ⾗Ḯേ‛ㆮવቇಽ㊁ጊਛ ਭ(ᩣ) ࠗ࠽࡝ࠨ࡯࠴ጊᧄ ඳንጊᄢቇ ↢๮⑼ቇవ┵⎇ⓥ࠮ࡦ࠲࡯ േ‛⾗Ḯ㐿⊒ಽ㊁ጊᧄ ᅢ↵

ਃ㊀ᄢቇ ␠ળㅪ៤⎇ⓥ࠮ࡦ࠲࡯ દ⾐⎇ⓥ᜚ὐᮮ੗ ષ⧷␹ᚭᄢቇ ᄢቇ㒮කቇ⎇ⓥ⑼ ಽሶઍ⻢කቇ㑐⷏ታ㛎േ‛⎇ⓥળ ળ㐳࡮ᐙ੐࡮⋙੐ฬ★
㧔ᐔᚑ 23㨪25 ᐕᐲ‫ޔ‬ᐔᚑ 25 ᐕ 10 ᦬⃻࿷㧕᳁ฬ ᚲዻ

ળ㐳⧔Ꮉᔘᄦ੩ㇺᄢቇᄢቇ㒮කቇ⎇ⓥ⑼੩ㇺ∔ᖚࡕ࠺࡞⎇ⓥᚲ

ᐼോ࡮ળ⸘

༑ᄙᱜ๺ ੩ㇺᐭ┙ක⑼ᄢቇᄢቇ㒮කቇ⎇ⓥ⑼ታ㛎േ‛࠮ࡦ࠲࡯ ᐶᧄ㜞ᔒ੩ㇺᄢቇᄢቇ㒮කቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳㓸ળ㧔ᐙ੐㐳㧕㒙ㇱᢅ↵ᳰ↰ථ਽ᣣᧄ࠴ࡖ࡯࡞ࠬ࡮࡝ࡃ࡯㧔ᩣ㧕ጟ↰೑਽ᄢ㒋ᐭ┙ᄢቇᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼ታ㛎േ‛ቇ



㤥Ỉദ



ਭ଻⮍ᄹ⦟⋵┙ක⑼ᄢቇవ┵කቇ⎇ⓥᯏ᭴േ‛ታ㛎ᣉ⸳㧔೽ᐙ੐㐳㧕᪀᧛ల

ᄢ㒋ᐭ┙ᄢቇᄢቇ㒮↢๮ⅣႺ⑼ቇ⎇ⓥ⑼₞ක∛ℂቇㄭ⮮₵੩ㇺᄢቇౣ↢ක⑼ቇ⎇ⓥᚲ㒝ዻౣ↢ታ㛎േ‛ᣉ⸳Ⴎ⷗㓷ᔒ␹ᚭᄢቇᄢቇ㒮කቇ⎇ⓥ⑼㒝ዻേ‛ታ㛎ᣉ⸳



↰ፉఝ



ဝ↰⵨มᄢ㒋ᴡ㦮࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦᄢቇ࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦቇㇱ ᧻↰Ả৻㇢ ⁛ ක⮎ၮ⋚⎇ⓥᚲ∔ᖚࡕ࠺࡞ዊേ‛⎇ⓥቶ᫪ᧄ⚐มᄢ㒋ක⑼ᄢቇᄢቇ㒮කቇ⎇ⓥ⑼⎇ⓥᯏ᭴ታ㛎േ‛࠮ࡦ࠲࡯

ᄢ㒋ᄢቇකቇㇱ㒝ዻേ‛ታ㛎ᣉ⸳ጊᷝ⵨ਯ૑෹ൻቇ ᩣ ↢‛ⅣႺ⑼ቇ⎇ⓥᚲ㧔೽ᐙ੐㐳㧕 ᮮ੗ષ⧷␹ᚭᄢቇᄢቇ㒮කቇ⎇ⓥ⑼ಽሶઍ⻢කቇ✬㓸㧔ᆔຬ㐳㧕  ጊᧄᅢ↵ਃ㊀ᄢቇ␠ળㅪ៤⎇ⓥ࠮ࡦ࠲࡯દ⾐⎇ⓥ᜚ὐਛ᧛⚩৻ᦶṑ⾐ක⑼ᄢቇേ‛↢๮⑼ቇ⎇ⓥ࠮ࡦ࠲࡯

ਛ੗િሶᣣᧄᣂ⮎ ᩣ ⎇㐿ડ↹⛔᜝ㇱ⋙੐ᷡ᳓⧷↵ᷡ᳓ታ㛎᧚ᢱ㧔ᩣ㧕ጊፒ┨ᒄࠝ࡝ࠛࡦ࠲࡞㉂ᲣᎿᬺ㧔ᩣ㧕᧲ᣣᧄࡃࠗࠝ༡ᬺㇱᐔᚑᐕᐲ෼ᡰ᳿▚ᦠᐔᚑᐕᐲ੍▚‫ޓޓ‬㊄㗵㧔౞㧕 ‫ޓޓ‬㊄㗵㧔౞㧕 ‫ޓ‬ኻᲧ㧔౞㧕

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䇭䇭ᐔᚑ㪉㪌ᐕᐲ䇭㑐⷏ታ㛎േ‛⎇ⓥળ੍▚ᦠ⾌⋡䇭䇭䇭➅⿧㊄ળ⾌෼౉䇭᥉ㅢળຬ⾌䋨ญ䋩䇭෼ 䇭⹏⼏ຬળຬ⾌䋨ญ䋩䇭౉ 䇭⛽ᜬળຬ⾌䋨࿅૕䋩䇭䈱 䇭ෳട⾌䋨㕖ળຬ䋩㩿ญ㪀䇭ㇱ ળႎઍ㊄
㗍㊄೑ᕷ
ᣏ⾌㄰㊄
෼౉⸘
䇭䇭䇭෼౉✚⸘

䇭ᡰ
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䇭䈱
䇭ㇱ

੐ോዪ⚻⾌
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䇭੐ോ↪ᶖ⠻ຠ⾌䇭ශ೚⾌ᯏ㑐⹹⊒ⴕ⚻⾌䇭✬㓸⾌䇭ශ೚⾌䇭ળ⹹㪧㪛㪝ൻ⚻⾌㓸ળ⚻⾌䇭ળ႐⾌䇭⻠Ṷ⠪᜗ᓙ⾌䇭ᐙ੐ળ䊶⹏⼏ຬળ䇭䉝䊦䊋䉟䊃⾌㩷੍஻⾌ᡰ಴䇭⸘ᐔᚑ㪉㪌ᐕᐲ੍▚
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䇭䇭➅⿧೾૛㊄



䇭䇭ᡰ಴✚⸘



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ᧂ౉㊄౞ࠍ฽߻

㑐⷏ታ㛎േ‛⎇ⓥળળೣΣ㧚✚ೣ㧔㧕ᧄળߪ㑐⷏ታ㛎േ‛⎇ⓥળ㧔-CPUCK.CDQTCVQT[#PKOCN4GUGCTEJ#UUQEKCVKQP㧕ߣ޿߁‫ޕ‬㧔㧕ᧄળߪ㑐⷏࿾඙ߦ߅޿ߡታ㛎േ‛ቇߥࠄ߮ߦ㑐ㅪ⻉⑼ቇߩ⊒㆐ࠍ࿑ࠆ੐ࠍ⋡⊛ߣߔࠆ‫ޕ‬㧔㧕ᧄળߪߘߩ⋡⊛ࠍ㆐ᚑߔࠆߚ߼ߦએਅߩ⻉੐ᬺࠍⴕߥ߁‫ޕ‬  Ԙቇⴚ㓸ળߩ㐿௅  ԙળ⹹ߩ⊒ⴕ  Ԛ㑐ଥ⻉ᯏ㑐࡮⻉ቇળߣߩᖱႎ੤឵࡮ㅪ⛊  ԛળຬ⋧੕ߩㅪ⛊  Ԝߘߩઁᔅⷐߣ⹺߼ࠄࠇࠆ੐ᬺΤ㧚ળຬ㧔㧕ᧄળߩળຬߪ୘ੱ߆ࠄߥࠆ᥉ㅢળຬߣᴺੱ෸߮ߎࠇߦḰߕࠆ࿅૕߆ࠄߥࠆ⛽ᜬળຬ߆ࠄߥࠆ‫ޕ‬㧔㧕ળຬߪᧄળߩ⿰ᣦߦ⾥หߒ‫ᧄޔ‬ળࠍ⛽ᜬߔࠆߚ߼ߦળ⾌ࠍᡰᛄ߁‫ޕ‬㧔㧕ળ⾌ߪ೨⚊ߣߒ‫ޔ‬᥉ㅢળຬߪᐕ㗵౞‫⼏⹏ޔ‬ຬߪ ౞‫⛽ޔ‬ᜬળຬߪ౞ߣߔࠆ‫ޕ‬㧔㧕ળຬߪળ⹹ߩ㈩ઃࠍฃߌࠆ‫ޕ‬㧔㧕ᧄળߦฬ⹷ળຬࠍ߅ߊߎߣ߇ߢ߈ࠆ‫ޕ‬Υ㧚ᓎຬ㧔㧕ᧄળߩᓎຬߪ‫ޔ‬ળ㐳ฬ‫⼏⹏ޔ‬ຬ⧯ᐓฬ෸߮⋙੐ฬߣߔࠆ‫ޕ‬㧔㧕ળ㐳ߪ⹏⼏ຬߩ੕ㆬߦࠃߞߡㆬ಴ߔࠆ‫ޕ‬㧔㧕⹏⼏ຬߪ᥉ㅢળຬฬએ਄ߩផ⮈ߦࠃߞߡㆬ಴ߒ‫✚ޔ‬ળߩᛚ⹺ࠍฃߌࠆ‫ޕ‬㧔㧕⋙੐ߪ⹏⼏ຬߩផ⮈ߦࠃߞߡㆬ಴ߒ‫ޔ‬ળ㐳߇ᆔབྷߔࠆ‫ޕ‬㧔㧕ળ㐳ߪᧄળࠍઍ⴫ߒ‫ޔ‬ળോࠍ⛔ℂߔࠆ‫ޕ‬ળ㐳ߦᡰ㓚߇޽ࠆߣ߈ߪ⹏⼏ຬߩ੕ㆬߦࠃࠅ ฬࠍㆬ಴ߒ‫ޔ‬   ળ㐳ߩ⡯ോࠍઍⴕߔࠆ‫ޕ‬㧔㧕ળ㐳ߪ⹏⼏ຬળࠍถ㓸ߒ‫⼏ߩߘޔ‬㐳ߣߥࠆ‫ޕ‬㧔㧕⹏⼏ຬߪ⹏⼏ຬળࠍ⚵❱ߒ‫ᧄޔ‬ળߦ㑐ߔࠆ㊀ⷐߥ੐㗄ࠍክ⼏࡮᳿ቯߔࠆ‫⼏⹏ޔߚ߹ޕ‬ຬߩ੕ㆬߦࠃࠅ   ㆬ߫ࠇߚᐙ੐ߪ‫ޔ‬ᐙ੐ળࠍ⚵❱ߒ‫ޔ‬ળ㐳ߩ⵬૒෸߮ᐼോ‫ޔ‬ળ⸘‫ޔ‬㓸ળ‫ޔ‬ળ⹹⊒ⴕߥߤߩળോࠍታⴕߔࠆ‫ޕ‬㧔㧕⋙੐ߪળ⸘ࠍ⋙ᩏߔࠆ‫ޕ‬㧔㧕ᓎຬߩછᦼߪᐕߣߒ‫ޔ‬ౣછࠍᅹߍߥ޿‫ޕ‬Φ㧚✚ળ㧔㧕ળ㐳ߪᲤᐕ࿁᥉ㅢળຬߢ᭴ᚑߐࠇࠆ✚ળࠍถ㓸ߒ‫ޔ‬ળോߩᔅⷐ੐㗄ࠍႎ๔ߒ‫ޔ‬ᛚ⹺ࠍฃߌࠆ‫ޕ‬Χ㧚ળ⸘㧔㧕ᧄળߩ੐ᬺᐕᐲߪᲤᐕ᦬ᣣࠃࠅ᦬ᣣ߹ߢߣߔࠆ‫ޕ‬㧔㧕ᧄળߩ⚻⾌ߪળ⾌‫ޔ‬ነઃ㊄ߘߩઁߩ෼౉ࠍ߽ߞߡ޽ߡࠆ‫ޕ‬Ψ㧚㒝ೣ㧔㧕ᧄળೣߪᤘ๺ᐕ᦬ᣣࠃࠅᣉⴕߔࠆ‫ޕ‬㧔㧕ᧄળೣߩᡷᱜߪ⹏⼏ຬળߩ⼏᳿ࠍ⚻ߡ✚ળߩᛚ⹺ࠍฃߌࠆ‫ޕ‬㧔㧕ᧄળߩ੐ോዪߪ੩ㇺᏒᏀ੩඙ศ↰ᴡේ↸ 14‫⽷⋉౏ޔ‬࿅ᴺੱ ੩ㇺᛛⴚ⑼ቇ࠮ࡦ࠲࡯ 22 ภቶߦ⟎ߊ‫ޕ‬Ω㧚㒝ೣ㧔㧕ᧄળೣߪᐔᚑᐕ᦬ᣣࠃࠅᣉⴕߒ‫ޔ‬ᐔᚑᐕ᦬ᣣࠃࠅㆡ↪ߔࠆ‫ޕ‬㧔㧕ᧄળೣߪᐔᚑᐕ᦬ᣣࠃࠅᣉⴕߒ‫ޔ‬ᐔᚑᐕ᦬ᣣࠃࠅㆡ↪ߔࠆ‫ޕ‬㧔㧕ᧄળೣߪᐔᚑᐕ᦬ᣣࠃࠅᣉⴕߒ‫ޔ‬ᐔᚑᐕ᦬ᣣࠃࠅㆡ↪ߔࠆ‫ޕ‬㧔㧕ᧄળೣߪᐔᚑ  ᐕ  ᦬  ᣣࠃࠅᣉⴕߒ‫ޔ‬ᐔᚑ  ᐕ  ᦬  ᣣࠃࠅㆡ↪ߔࠆ‫ޕ‬