㪉㪇㪈㪉䋮㪈㪉䋮㪇㪌㱎䉫䊦䉦䊮ද⼏ળ㱎䉫䊦䉦䊮䈱ᯏ⢻ᕈ䈫䈠䈱ᔕ↪

㤥㉂Უ↱᧪Ǫ㧰ࠣ࡞ࠞࡦ̌ࠕࠢࠕǪ㨀㧹̍ߩᯏ⢻ᕈߦߟ޿ߡ࠳ࠗ࠰࡯ᩣᑼળ␠㧾㧒㧰ᧄㇱ 㐿⊒ડ↹ㇱ
㋈ᧁ ೑㓶ౝኈ䉝䉪䉝㱎TM䈮䈧䈇䈩䉣䊎䊂䊮䉴䊃䊏䉾䉪䉴䉝䊧䊦䉩䊷ᡷༀലᨐ䉴䊃䊧䉴䈱ᡷༀ
HSP70䈱⺃ዉ㉄ᕈൻ♆ຠ䈻䈱ᔕ↪

E-䉫䊦䉦䊮䇸䉝䉪䉝E70䇹䈮䈧䈇䈩䉝䉪䉝㱎TM 䋨㜞⚐ᐲ♖⵾㱎-1,3-1,6䉫䊦䉦䊮 䋩• 㤥㉂Უ⩶Aureobasidium pullulans 1A1ᩣ䈮䉋䉍⊒㉂↢↥• 㱎-1,3⚿ว䈱ਥ㎮䈫㱎-1,6⚿ว䈱㜞ಽጘ䉫䊦䉮䊷䉴஥㎮• ⩶૕ᄖಽᴲ䈘䉏䉎䈢䉄นṁᕈ• 㜞⚐ᐲ80%એ਄
• ᳓ṁᕈ䇮ᓸ☸ሶ᭴ㅧ• ૐ☼ᐲൻಣℂ㱎-䉫䊦䉦䊮E-1,6⚿วE-1,3-⚿วa>0.9㱎㵥䉫䊦䉦䊮䋨䉝䉪䉝㱎TM䋩䈱⣺䈻䈱௛䈐䈎䈔㱎㵥䉫䊦䉦䊮䋨䉝䉪䉝㱎TM䋩఺∉ᵴᕈ䈮䈧䈇䈩
IgA↥↢㊂䂾⣺▤఺∉ᵴᕈൻ䈫⺞▵૞↪

䉫䊦䉦䊮⚻ญᛩਈ⟲䈪䇮IgA᛫૕䉇IFN-J䈭䈬䈱䉰䉟䊃䉦䉟䊮↥↢㊂䈏Ⴧട⣺▤఺∉䈱⾮ᵴ䊶⺞▵⥄ᓞ␹⚻䈻䈱௛䈐䈎䈔⢗⣺☼⤑䈱଻⼔⊹⤏䈱ᯏ⢻ᡷༀ఺∉ജ䈱䉝䉾䊒᛫⣲≌䊶᛫䈏䉖ォ⒖䉝䊧䊦䉩䊷䈱ૐᷫ䉴䊃䊧䉴䈱ૐᷫ
ଢ⒁䈱ᡷༀലᨐ㘩ᓟⴊ♧䈱ૐᷫ䊜䉺䊗ᡷༀਅ∯䈱ૐᷫ⢗ẩ≌ૐᷫㆊᢅᕈᄢ⣺Ἳ䈱ᡷༀ
HSP䋷䋰䈱⺃ዉ଻Ḩᯏ⢻䉝䉾䊒䊋䊥䉝ᯏ⢻ᡷༀ⡼䈱䊊䊥ᡷༀ䉝䉪䉝E

㪈㪉㪇㪇

IgA↥↢㊂ (ng/ml)䊙䉡䉴䈮䉫䊦䉦䊮⚻ญᛩਈᓟ䇮䊌䉟䉣䊦᧼䈪䈱
IgA᛫૕䇮ฦ⒳䉰䉟䊃䉦䉟䊮↥↢㊂䉕᷹ቯ䉝䉪䉝㱎䌔䌍

↢ℂ㘩Ⴎ᳓

㪈㪋㪇㪇

䋨ᣣᧄᄢቇ↢‛⾗Ḯ⑼ቇㇱ ਄㊁Ꮉᢎ᝼䋩㪈㪇㪇㪇㪏 㪇㪇㪍 㪇㪇㪋 㪇㪇㪉 㪇㪇㪇㋈ᧁ㓉ᶈ䉌䇮ᣣᧄㄘ⧓ൻቇળᄢળⷐᣦ㓸 Pg.279 (2005)

㱎㪇㱎㪌 㪇ၭ㙃ᤨᷝട䉝䉪䉝E㊂ (Pg/ml)

Suzuki et al, Proceedings of JAACT International Meeting (2004).

E-1,6⚿วE-1,3-⚿ว

a>0.9

Suzuki et al., Food Function, 2, 45-50 (2006).

䉝䉪䉝㱎㪫㪤䈱䊍䊃䈪䈱ലᨐ㪊㪅㪇䋨䉲䊮䉫䊦䊑䊤䉟䊮䊄䋲⟲㑆ਗⴕᲧセ⹜㛎䋩㪉㪅㪇⢈ḩ⚦⢩㪈㪉㪇㪏㪇㪇㑆ᓟข៨៨㑆ᓟ㑆ᓟข㪏ㅳ㪎ㅳ㪍ㅳ㪌ㅳ⧎☳㊂㑆ᓟ៨៨ขข㪏ㅳ㪎ㅳ㑆ᓟ㑆ᓟ㑆ᓟ㪍ㅳขข៨៨៨ข㪌ㅳ㪋ㅳ㑆ᓟ㑆ᓟ㑆ᓟข៨៨ข㪊ㅳ㪉ㅳ㪈ㅳข៨៨㑆ᓟ೨㐿ᆎ㪉㪇㪈㪉㪆㪋㪆㪋㪉㪇㪈㪉㪆㪋㪆㪈㪏㪉㪇㪈㪉㪆㪋㪆㪈㪈㪉㪇㪈㪉㪆㪊㪆㪉㪏㪉㪇㪈㪉㪆㪊㪆㪎㪉㪇㪈㪉㪆㪊㪆㪉㪈㪉㪇㪈㪉㪆㪊㪆㪈㪋㪉㪇㪈㪉㪆㪉㪆㪉㪐㪉㪇㪈㪉㪆㪉㪆㪏㪉㪇㪈㪉㪆㪉㪆㪉㪉㪉㪇㪈㪉㪆㪉㪆㪈ข㧙  㧙㪈㪉㪇ᛩਈ⟲㕖ᛩਈ⟲ᐔဋ⧎☳㊂㪈㪇㪇㪏㪇㪈㪅㪌㪍㪇㪈㪅㪇㪋㪇ᓟᓟ㑆㪏ㅳ㪎ㅳ㑆៨ข㪍ㅳ㑆៨ขᓟ㪌ㅳ㑆៨ข៨ข㪋ㅳ㑆㑆ᓟᓟ㑆㑆ᓟ㪇ᓟ㪉㪇㪇㪅㪇ᓟ㪇㪅㪌⧎☳㊂㪉㪅㪇៨ข䉝䉪䉝㱎䈱㊂䋨න࿁ᛩਈ䋩ᶡ㪉㪅㪌㪊ㅳ䉝䉪䉝㱎䈱㊂䋨฽᦭㘩㙄䋩

Kimura et al, International Immunopharmacology,7, 963-972 (2007).

㪉㪇㪈㪉㪆㪉㪆㪈㪌⚿ᨐ䋺䊶䈒䈚䉆䉂䇮㥦᳝䇮䈭䉂䈣䇮ᣣᏱ↢ᵴ䈱ᡰ㓚ᐲ䈭䈬䈪⧎☳∝シᷫ䊶䉴䉩⧎☳䈱㘧ᢔᤨᦼ䈫ⵍ㛎⠪䈱⧎☳∝⊒∝ᤨᦼ䈍䉋䈶䈠䈱∝⁁䈮䈲㜞䈇⋧㑐䊶⧎☳㘧ᢔ㊂䈱Ⴧട䈮઻䈉∝⁁䈱ᖡൻ䉕ᛥ೙䊶⧎☳∝䈮ኻ䈜䉎᦭ലᕈ⏕⹺㪉ㅳ㪈㪅㪇㪇㩼䊄䊥䊮䉪៨ขᦼ㑆䋨䋸ㅳ㑆䋩៨ข㪇㪅㪌㪇㩼㪇៨ข㪇㪅㪉㪌㩼㪏㪇㪉㪇㪈ㅳ

㪚㫆㫅㫋㫉㫆㫃⹜㛎ታᣉᦼ㑆㪈㪇㪇㪍㪇㪋㪇೨㪥㫆㫉㫄㪸㫃㪇

ᆎ㪇㪌㪇㪈㪉㪇

ᛩਈ⟲㕖ᛩਈ⟲ᐔဋ⧎☳㊂㐿

㪈㪈㪇㪇䈒䈚䉆䉂㪈㪇㪅㪇㪐㪅㪇㪏㪅㪇㪎㪅㪇㪍㪅㪇㪌㪅㪇㪋㪅㪇㪊㪅㪇㪉㪅㪇㪈㪅㪇㪇㪅㪇៨ข㪉㪈㪌㪇៨ข䋪㪊䉴䉩㘧ᢔ㊂㩿☳㪆㪺㫄㪉㪀㪉㪇㪇

J ippo et al.,Biosci. Biotechnol. Biochem,76, 84-88 (2012).

㪉㪇㪈㪉㪆㪈㪆㪉㪌㪋䊍䉴䉺䊚䊮䈭䈬䈱⋧ኻ᡼಴㊂䋨䋦䋩ෆ⊕䉝䊦䊑䊚䊮․⇣⊛㪠㪾㪜䈱㊂㪦㫍㪸㪄㪸㫃㪹㫌㫄㫀㫅㪄㫊㫇㪼㪺㫀㪽㫀㪺㩷㪠㪾㪜㪉㪌㪇㪌៨៨៨㪊㪇㪇㪍ข㑆ᓟ㪋ㅳข㑆ᓟ㪊ㅳ㪉ㅳ៨ขขข㑆ᓟ㐿ᆎ㪈ㅳขข៨㪊㪌㪇⧎☳∝䈮ലᨐ㘩‛䉝䊧䊦䉩䊷䈮ലᨐ㑆ᓟ㪉㪇㪇㪅㪇㑆ᓟ㪋㪇㪇㪅㪌೨㪍㪇㪈㪅㪇⧎☳㊂㪈㪇㪇㪈㪅㪌៨ᣇᴺ䋺䊶䋶䋳ฬ䈱⧎☳∝䉕᦭䈜䉎ⵍ㛎⠪䇮䉝䉪䉝㱎ᛩਈ⟲䈫㕖ᛩਈ⟲䈱䋲⟲䊶⹜㛎ᦼ㑆12ㅳ䋨2᦬䈎䉌4᦬䈱⧎☳㘧ᢔᤨᦼ䈮ว䉒䈞䈩ታᣉ䋩䊶ታᣉᯏ㑐 ៨ข೨ᦼ㑆䋨2ㅳ䋩䇮៨ขᦼ㑆䋨䋸ㅳ䋩䇮៨ขᓟᦼ㑆䋨䋲ㅳ䋩䊶䉝䊧䊦䉩䊷∝⁁ᣣ⹹䈱⸥㍳䇯⹏ଔ㗄⋡䋻䈒䈚䉆䉂䇮㥦᳝䇮㥦㐽䇮⌒䈱䈎䉉䉂䇮䈭䉂䈣䇮ᣣᏱ↢ᵴ䈱ᡰ㓚ᐲ㥦䈨䉁䉍ᛩਈ⟲㕖ᛩਈ⟲ᐔဋ⧎☳㊂㪉㪅㪌៨䉝䉪䉝㱎TM䈱Iဳ䉝䊧䊦䉩䊷ૐᷫലᨐ䊙䉡䉴䉕↪䈇䈢᜔᧤䉴䊃䊧䉴䈮䉋䉎ᓇ㗀
Kimura et al., Journal of Pharmacy and Pharmacology,59䋬1137-1144 (2007).

䊶䊙䉡䉴䉕7ᣣ㑆㘺⢒䈚䇮䋱ᣣ䋱࿁䇮ⵍ⷗‛⾰䋨᳓ or 䉝䉪䉝㱎䋩䉕ᛩਈ䇯
䊶3, 5, 7ᣣ⋡䈮12ᤨ㑆䈱᜔᧤䉴䊃䊧䉴䉕ਈ䈋䉎䇯
5d3d᜔᧤䉴䊃䊧䉴᜔᧤䉴䊃䊧䉴᜔᧤䉴䊃䊧䉴⽶⩄䊙䉡䉴䈮䈍䈔䉎ⴊਛ䉮䊦䉼䉮䉴䊁䊨䊮୯䈫㪥㪢ᵴᕈ䈻䈱ᓇ㗀
Kimura et al., Journal of Pharmacy and Pharmacology,59䋬1137-1144 (2007).

7d᜔᧤䉴䊃䊧䉴ⴊᶧ㪈㪇䋪

☨ P<0.05(B) ⥄↱ⴕേ + ៨㘩೙㒢(C) ⴕേ೙㒢 + ៨㘩೙㒢䌎䌋ᵴᕈ(A) ⥄↱ⴕേ + ⥄↱៨㘩䋪䋪㪥㪢ᵴᕈ㪏㪍㪋㪉+ 䇸䉝䉪䉝E䇹㫂㪾䋩
㪾㪆

㫂㪾䋩㪾㪆

䋨㪈
䊮䉦
䊦

䊦

䉫䉫

䊦
㱎

䉫㱎

㪇㪇
㫄

㪾㪆㪇㫄
䋨㪌
䊮䉦䉦

᧤䊮䉴䋨㪉䊃䊧㪌㫄Ᏹᱜ᜔7ᣣ⋡䈮䊙䉡䉴䈎䉌ណⴊ䉕ⴕ䈇䇮ⴊਛ䉮䊦䉼䉮䉴䊁䊨䊮Ớᐲ䉕᷹ቯ㱎⟲⩄㫂㪾䋩㪇䉴
⽶(D) 䉝䉪䉝E 25mg/kg(E) 䉝䉪䉝E 50mg/kg(F) 䉝䉪䉝E 100mg/kg㱎䉫䊦䉦䊮ᛩਈ⟲೽⣢⊹⾰䈪วᚑ䈘䉏䉎೽⣢⊹⾰䊖䊦䊝䊮䇯ᕆᕈ䈱䉴䊃䊧䉴䉕⽶⩄䈜䉎䈫⋥䈤䈮਄᣹䈜䉎䇯䊍䊃䈪䈱᛫䉴䊃䊧䉴䋨⚿ᨐ䋩䊍䊃䈪䈱᛫䉴䊃䊧䉴䋨⹜㛎ᣇᴺ䋩⹜㛎ౝኈⵍ㛎⠪䈲䇮䋱ᣣ䋱ᧄ䈱䊄䊥䊮䉪䋨䉫䊦䉦䊮 or 䊒䊤䉶䊗䋩䉕䇮14ᣣ㑆ㅪ⛯䈪៨ข䈜䉎䇯៨ขᦼ㑆೨䇮ਛ䇮ᓟ䈮䇮⥄ⷡ⊛䈭∝⁁䉕␜䈜䉝䊮䉬䊷䊃䋨VAS䋩䈫䇮ໜᶧᚑಽ⹜㛎䉕ታᣉ䇯

VAS
ໜᶧᬌᩏ

∋ഭᗵ䋨㪭㪘㪪䋩

䉮䊦䉼䉹䊷䊦㊂䋨㱘㪾㪆㫄㫃䋩

䌐䋼0.05䋦

㪇㪅㪋

㪁㪇㪅㪊㪌
㪇㪅㪊

㪁㪇
㪄㪇㪅㪉

㪄㪇㪅㪍㪇㪅㪉

䌐䋼0.05䋦

㪁㪁

㪇㪅㪉

㪄㪇㪅㪋

㪇㪅㪉㪌

䉫䊦䉦䊮⟲
䊒䊤䉶䊗⟲

䌐䋼0.01䋦

㪇㪅㪋

ᗵ
ഭ
∋

VAS
ໜᶧᬌᩏ

㪇㪅㪋㪌

⊛
૕
りVAS
ໜᶧᬌᩏ

ⷰኤᦼ㑆(7d)㪇㪅㪍䉫䊦䉦䊮⟲
䊒䊤䉶䊗⟲

ᗵ
ഭ
∋

VAS

䊄䊥䊮䉪៨ขᦼ㑆(14d)㪇㪅㪏㪇㪅㪌

⊛૕ోⷰኤᦼ㑆(7d)៨ข䋲ㅳ䉴䊃䊧䉴㪭㪘㪪䉝䊮䉬䊷䊃䉮䊦䉼䉹䊷䊦㊂ផ⒖2⹜㛎඙䉪䊨䉴䉥䊷䊋䊷⹜㛎䋺ⵍ㛎⠪䈮䈲䊄䊥䊮䉪䈱ౝኈ䉕ᢎ䈋䈝䇮Term䈪䊄䊥䊮䉪䈏౉䉏ᦧ䉒䉎
Term1 䋺 䉫䊦䉦䊮⟲ 14ੱ 䊒䊤䉶䊗⟲ 14ੱ
Term2 䋺 䊒䊤䉶䊗⟲ 14ੱ 䉫䊦䉦䊮⟲ 14ੱ
Term

䋺12㗄⋡

VAS (⥄ⷡ⊛䉴䊃䊧䉴)ໜᶧਛ䈱䉴䊃䊧䉴䊙䊷䉦䊷䋨ઁⷡ⊛䉴䊃䊧䉴䋩㪄㪇㪅㪏៨ข㪇ㅳ៨ข䋲ㅳ៨ขᤨ㑆

VAS䉴䊃䊧䉴⹏ଔ㗄⋡䉫䊦䉦䊮䉕2ㅳ㑆៨ข䈚䈢⚿ᨐ䊶⥄ⷡ⊛䉴䊃䊧䉴䉝䊮䉬䊷䊃䈮䈍䈇䈩䇮∋ഭᗵ䈮㑐䉒䉎㗄⋡䈮シᷫലᨐ䈏⷗䉌䉏䈢䇯䊶ໜᶧਛ䈱䉴䊃䊧䉴䊙䊷䉦䊷䈮䈍䈇䈩䇮䉮䊦䉼䉹䊷䊦Ớᐲ䈏ᷫዋ䈚䈢䇯㪭㪘㪪䋺 䉴䊃䊧䉴䈮㑐䈜䉎㪈㪉㗄⋡䈮䈧䈐䇮✢ಽ਄䈮ශ䉕౉䉏䉎⥄ⷡ⊛䈭⹜㛎ໜᶧ⹜㛎 䋺 ◲න䈭⸘▚໧㗴䉕ታᣉ䇮䈠䈱ᓟ䈮䇮ໜᶧ䉕ណข䈚䇮䉴䊃䊧䉴䊙䊷䉦䊷‛⾰䈱ቯ㊂䊍䊃䈪䉅䇮䉴䊃䊧䉴䉕シᷫ䈘䈞䉎ലᨐ䉕ታ⸽᜔᧤䉴䊃䊧䉴ଐሽ䈱ឃଢᢙჇട
䈮ኻ䈜䉎E-glucan䈱ലᨐ⒳䇱䈱⮎೷䈪⺃⊒䈚䈢ឃଢᢙჇട䈮ኻ䈜䉎E-glucan䈱ലᨐ

Asano et al.,䇮Biochemical and Biophisical Research Communications, 420, 444-449 (2012).

Asano et al.,䇮Biochemical and Biophisical Research Communications, 420, 444-449 (2012).

Bethanecoln=5-11n=6Serotonin(5-HT)**

Castor oil

n=4-5n=6*****n.s.

PBS

E-glucan(mg/kg)

Sham 600

PBS60

200PBS E㺍glucan

Bethanecol (3 mg/kg)

600

PBS

PBS E㺍glucan
5-HT (3 mg/kg)

PBS

PBS E㺍glucan
Castor oil(300 Pl/mouse)(**, P<0.01)
᜔᧤䉴䊃䊧䉴

(*, P<0.05; **, P<0.01)

㧙  㧙䉝䉪䉝㱎TM䈱ᣂ䈢䈭ᯏ⢻䊃䊏䉾䉪䉴⢗ẩ≌䈮ኻ䈜䉎E-glucan䈱ലᨐ
Mizushima et al.,The British Journal of Nutrition 106: 475-85 (2011).

⣺▤఺∉䈱⾮ᵴ䊶⺞▵䉴䊃䊧䉴ૐᷫ䇮㘩ᓟⴊ♧䈱ૐᷫ䊜䉺䊗ᡷༀ䇮ଢ⒁䈱ᡷༀH&E
ᨴ⦡

E-glucan䈲⢗ẩ≌䉕ᛥ೙䈜䉎䈎?
100% EtOH+ Eglucan (200)

100% EtOHᣂ䈢䈭ᯏ⢻䈱⊒⷗㪟㪪㪧䋷䋰䈱⺃ዉ䊶↥↢⢗⣺☼⤑䈱଻⼔⟤ኈ䊶᛫ട㦂ਅ∯䈱ૐᷫ⢗ẩ≌ૐᷫㆊᢅᕈᄢ⣺Ἳ䈱ૐᷫ଻Ḩᯏ⢻䉝䉾䊒䇮䊋䊥䉝ᯏ⢻ᡷༀ⡼䈱䊊䊥ᡷༀ

Mizushima et. al., British Journal of Nutrition, 106, 475-485 (2011).

䋪䋪

vehicleEglucan (200)≌ଥᢙ (mm2)䊚䉣䊨䊕䊦䉥䉨䉲䉻䊷䉷ᵴᕈ(μmol H2O2 /min/mg protein)⢗☼⤑៊்䈱੍㒐ലᨐ

E-glucan(mg/kg)

100% EtOH

䋪

0520

⸃⺑䋺
䊶HSP䈫䈲䋻䉺䊮䊌䉪⾰䉕቞䉍䇮䉺䊮䊌䉪⾰䉕⢒䉃䇸䉺䊮䊌䉪⾰䇹䊶䈅䉌䉉䉎䉴䊃䊧䉴䈎䉌䉺䊮䊌䉪⾰䉕቞䉎䇸䉺䊮䊌䉪⾰䇹䇮䈠䉏䈏HSP䈪䈅䉍ಽሶ䉲䊞䊕䊨䊮䈣䋪

50 100 200

䊶HSP䈱ක⮎䇮ൻ♆ຠ䇮㘩ຠ⚛᧚䈮㑐䈜䉎⍮⷗䈏ḩタ100% EtOH

䊶䉻䉟䉸䊷␠⵾䈱㱎䉫䊦䉦䊮䇸䉝䉪䉝㱎TM䇹䈏䇮HSP䈱↥↢⺃ዉ䉕ଦㅴ䈜䉎䈖䈫䈏⸥タ䈘䉏䈩䈇䉎䇯䋲䋰䋱䋲ᐕ 䋶᦬䋲䋱ᣣ ಴䋳㊀ⲷᣓ᭴ㅧ䊋䉟䉥↱᧪᳓♽䊅䊉ಽᢔ೷

䋨Limulus negative䋩ⅣႺᄌൻ䋨䉝䊦䉦䊥䉝䊦䉦䊥DMSOዩ⚛
㜞᷷䋱㊀ⲷᣓ᭴ㅧ䋨቟ቯဳ䋩䋱㊀ⲷᣓ᭴ㅧ䋨ਇ቟ቯဳ䋩ਛᕈ䋩ਇṁᕈ䊅䊉☸ሶ䉇ಽሶ䊤䊮䉻䊛䉮䉟䊦
1㊀ⲷᣓ/䊤䊮䉻䊛᭴ㅧ3㊀ⲷᣓ᭴ㅧ㱎㪄䉫䊦䉦䊮䈱㜞ᰴ᭴ㅧ䈱ᄌൻ
E-1,6⚿วE-1,3-⚿ว

100nmnm100a>0.9

HV=100kv
HV=100kv
DirectMag:20000x
DirectMag:20000x

䉝䉪䉝㱎㪫㪤൮ធ䈚䈢㉄ൻ䉼䉺䊮☸ሶ䋨TEM↹௝䋩㧙  㧙䉝䉪䉝㱎㪫㪤൮ធ᳓㉄ൻ䉝䊦䊚ಣℂ㉄ൻ䉼䉺䊮䈱᳓ಽᢔᶧ䋨pH7.1䋩

䊋䉟䉥↱᧪᳓♽䊅䊉ಽᢔ೷䉝䉪䉝㱎TME-1,6⚿วE-1,3-⚿วa>0.9䊅䊉䊙䊁䊥䉝䊦ක≮᧚ᢱ
DDS䈭䈬

Thank you for your kindly attention !!
䈗ᷡ⡬䉕ᗵ⻢䈚䉁䈜䇯㧙  㧙ᯏ⢻ᕈ㘩ຠ⚛᧚⣺▤఺∉⢗⣺䈱଻⼔ൻ♆ຠ⚛᧚଻Ḩ᛫Ἳ∝