BioSpec-nanoC101-E112D

Spectrophotometer for Life Science

BioSpec-nanoBioSpec-nanoSpectrophotometer for Life Science

Drop-and-Start Analysis
Automatic Optical Pathlength Setting & Automatic Wiping
Low Carryover Achieved with Automatic Wiping Feature
Quick & Simple Operation
Outstanding Measurement Accuracy& Reproducibility

P. 4
P. 5
P. 5
P. 6
P. 7

Stress-free Nucleic Acid
& Labeled Nucleic Acid Quantitation
Outstanding Analysis Range & Measurement Reproducibility
Photometric Measurement
BioSpec-nano Specifications

P. 8
P. 9
P. 10P. 11

With a focus on ease of use, high-accuracy
quantitation of nucleic acids andproteins has become quicker and faster.
In addition, photometric measurement is now possible.

1 µL or 2 µL Nucleic Acid Quantitation Note1)
Analysis can be performed with 1 µL (pathlength: 0.2 mm) or 2 µL (pathlength: 0.7 mm) samples.

Easy Drop-and-Start Measurement
Automatic sample mounting eliminates the need for arm raising and lowering,and the automatic wiping feature makes wiping of the measurement sample
unnecessary, enabling measurement of one sample after another.

Excellent Reproducibility and Measurement Accuracy
High reproducibility is obtained even when measuring low concentration samples.
High correlation is achieved with the measurement values provided with the double
beam spectrophotometer.

Quick & Simple Operation
Blank measurement, sample measurement, output of reports as
PDF or CSV files, and other basic operations are performed quickly
and simply with a click of a button.

Stress-Free Analysis with Data Judgment Feature
Automatic data judgment based on the OD800 measurement value
eliminates the need for concern about inadequate measurement caused
by a drop in volume or the inclusion of bubbles.

Note 1) When measuring substances like proteins that do not easily form droplets, deposit more than 1 – 2 µL of sample.

Drop-and-Start Analysis [1 µL / 2 µL samples can be measured.]Note2)

Just drop the sample onto the target and click the button. That's all there is to analysis. The instrument will perform everything else foryou – sample mounting, measurement and wiping. Liquid-contact parts no longer need to be wiped with a cloth.

1. Drop the sample
Sample volume required formeasurements are :1 µL for pathlength 0.2 mm
2 µL for pathlength 0.7 mm

2. Outstanding analysis range &measurement reproducibility
The optimum pathlength for thesample concentration can be selectedusing the instrument lever.

3. Start measurement
Click the [start] button to start sample
measurement.
You will find using the [start] button on
the instrument handy when performing
analyses with gloves on.

Automatic sample wiping
allows the analyst todeposit the next
measurement sample while
the current sample is being
measured without having
to put down the pipette.
Measurement is easy evenwhen there are manysamples.

Button in software
window

Instrument [start] button

4. Check the analysis results
Analysis results are automaticallydisplayed after measurement ends.
A series of samples can be analyzed
while confirming spectra in theDetailed View Mode.

[Simple Nucleic Acid Quant. - Detailed View Mode][Measurement Sample: Purified dsDNA in Tris-EDTA (TE) buffer]
Note 2) When measuring substances like proteins that do not easily form droplets, deposit more than 1 – 2 µL of sample.
The results can be checked using the analysis data judgment feature. (See P. 7)

4Automatic Optical Pathlength Setting & Automatic Wiping
From setting the optical pathlength to measurement to wiping away the sample, it’s all automatic.
Tedious operations, like raising and lowering the arm to set the pathlength and wiping off the sample with a piece of cloth, are no longer
required. There is no need to worry about whether the sample has been thoroughly wiped away, or whether the measurement site might
become scratched.

Depositing sample

Upper window
TargetLowering window and measurement

Sample wiping and raising window

Low Carryover Achieved with Automatic Wiping Feature
The automatic wiping function of the BioSpec-nano greatly
minimizes sample carryover from one measurement to the next.
Below are the results of alternate measurement of a double
stranded DNA sample and a blank (TE buffer), each measured 60
times with automatic one-time wipingNote 3) between

measurements. In order to determine the level of carryover fromthe measured DNA sample, measurement of the next blank wasconducted without replacing the wiper during the analyses. The
results indicated that carryoverNote 4) was extremely low, averaging amere 0.13%.

Note 3) Automatic wiping following measurement can be set to a maximum of 5 times. Normally, wiping just once is sufficient to obtain goodresults, however, wiping 3 times is recommended for samples having high viscosity, such as protein samples.
Note 4) Carryover (%) = (calculated DNA concentration when measuring TE buffer / concentration of DNA measured immediately before) x 100.

Measurement Values from 60 Alternate Measurements Each of Double Stranded DNA Sample and Blank (TE Buffer)

OD Value151050020406080100120

Number of Measurements

BioSpec-nanoSpectrophotometer for Life Science5Quick & Simple Operation
Basic operations can be conveniently performed by clicking icons in the software or function keys on the instrument itself.
Organizing data is also easy even when there many samples.

Start

Blank

Display of Measurement Results

Detail /List

Toggles between the Detail and List view modes.
DetailDisplays the analysis results and spectrum of the sample currently selected.

List

Displays the analysis results of all samples in a table format.
Converts analysis results to PDF files, and displays them using Adobe Reader.

Saves Analysis Results
Saves analysis results to CSV or PDF files. CSV files can be edited in Microsoft Excel or other spreadsheet software.

Display PDF

6Save CSV/PDF

Outstanding Measurement Accuracy & Reproducibility[Secure Analysis / High Measurement Accuracy]
As little as 1 – 2 µL of undiluted sample can be measured as is.
High reproducibility is obtained even when measuring low concentration samples.
High correlation is achieved with the measurement values provided with the double beam spectrophotometer.

Example of 10 Successive Measurements of the Same Sample (Double Stranded DNA)
Pathlength: 0.2 mm (Example of analysis at 1 µL)
CV 0.84 % with OD Value 40(Concentration: 2,000 ng/µL)
Measured OD260

Measured OD260

40200Pathlength: 0.7 mm (Example of analysis at 2 µL)

5CV 1.4 % with OD Value 1.5(Concentration: 75 ng/µL)
00204005

Correct OD260

Correct OD260

Sample: Diluted purified double stranded DNA
Buffer: TEInstrument : Shimadzu double beam spectrophotometer

Measurement using 1 mm cell and 0.1 mm cell.

Example of 10 Successive Measurements of the Same Sample (BSA = Bovine Serum Albumin)
Pathlength: 0.2 mm (Example of analysis at 3 µL)

Pathlength: 0.7 mm (Example of analysis at 4 µL)
8CV 1.05 % with OD Value 43(Concentration: 65,000 µg/mL)
Measured OD280

Measured OD280

4020

CV 0.98 % with OD Value 4(Concentration: 6,060 µg/mL)

6420020

40

Correct OD280
Sample: BSA
Buffer: Distilled water
Instrument : Shimadzu double beam spectrophotometer

002468Correct OD280

Measurement using 1 mm cell and 0.1 mm cell.

Analysis Data judgment
If a droplet is not properly formed for some reason, such as because of, the depositing of insufficient volume, or if the sample contains
bubbles, a larger OD800 value is generated. If the measured value is outside the range of the set OD800 value,
is displayed,allowing confirmation of the measurement validity.
(Note: This applies to sample solutions that do not exhibit absorption at 800 nm.)

BioSpec-nanoSpectrophotometer for Life Science7

Stress-free Nucleic Acid & Labeled Nucleic Acid QuantitationSimple Nucleic Acid Quantitation Mode
For the quantitation of RNA, dsDNA, ssDNA,and OligoDNA.
The nucleic acid concentration and OD ratio(OD260/280 and OD260/230) are calculated.

[Simple Nucleic Acid Quant. - Detailed View Mode][Measurement sample: Purified dsDNA in TE buffer][Analysis Setting Windows - Simple Nucleic Acid Quant.]

Labeled Nucleic Acid Quantitation Mode
For the quantitation of nucleic
acids (RNA, dsDNA, ssDNA,OligoDNA) labeled by Cy3, Cy5,
Alexa Fluor 546, and Alexa Fluor
647Note5).
The nucleic acid concentration,label concentration, nucleotide
concentration, and labeling ratio
are all calculated.

[Labeled Nucleic Acid Quant. - Detailed View Mode][Measurement Sample: OligoDNA labeled by Cy3 in TE buffer][Analysis Setting Windows - Labeled Nucleic Acid Quantitation]

8Note 5) Four dyes are pre-registered. An additional 8
new labels can be registered.

Outstanding Analysis Range & Measurement Reproducibility
Protein Quantitation Mode
The protein concentration iscalculated from the molar
absorption coefficient.

[Protein Quantitation (OD280 Method) - Detailed View Mode](Measurement Sample: BSA)

[Analysis Selection Window - Protein Quantitation (OD280 Method)]Labeled Protein Quantitation Mode
Cy3, Cy5, Alexa Fluor 546, andAlexa Fluor 647Note 5) are used forquantitation of labeled proteins.
The protein concentration, label
concentration, and labeling rate arecalculated.

[Labeled Protein Quantitation - Detailed View Mode](Measurement Sample: Alexa Fluor 546-IgG)[Analysis Selection Window - Labeled Protein Quantitation]

BioSpec-nanoSpectrophotometer for Life Science9Photometric Measurement
Photometric Measurement ModeOD values can be displayed for up to 8 freely selectedwavelengths.

[Photometric Measurement - Detailed View Mode](Measurement Sample: BSA)

[Analysis Selection Window - Photometric Measurement]

Option Cell (Pathlength: 5 mm)

Measured OD260

The 5 mm pathlength cell and 5 mm cell adapter are
required separately.
The optional 5 mm pathlength cell and its adapter areplaced over the target for use as shown below.

Correct OD260

Example of analysis at 2 mL** All of the analysis example data are provided for reference only.
1 OD corresponds to 50 ng/µL dsDNA.
Sample: Purified dsDNA
Buffer: TE
5 mm pathlength cell(sample volume: 2 mL)(P/N: 208-92258)

105 mm cell adapter(P/N: 206-26513)

Ten measurements were performed at each point.

BioSpec-nano Specifications
Hardware Specifications
Item

Specification

Item

Specification

Measuring wavelength range

220 to 800 nm

Detector

Photo diode array

Spectrum bandwidth

3 nm

Auto wiping function

Provided

Wavelength accuracy±1 nm

Sample mount function

AutoPathlength

0.2 mm, 0.7 mm (manual selection)

Spectrum measuring time

3 secPhotometric range

0 to 1.5 Abs

Quantitation range*

Pathlength 0.2 mm, 1 to 75 OD 50 to 3,700 ng / µL

Photometric value unitOD (Optical Density), absorbance converted with 10 mm pathlength(OD, dsDNA concentration)

Pathlength 0.7 mm, 0.3 to 21 OD 15 to 1,000 ng / µL

Option cell

Available (pathlength: 5 mm, sample volume: 2 mL)
Pathlength lever switched to Option (5 mm) position

Sample volume

Optional 5 mm pathlength cell, 0.04 to 3 OD
Power requirements

1 µL min. (pathlength: 0.2 mm)

2 to 150 ng / µL
AC 100 V / 120 V / 220 V / 230 V / 240 V, 50 / 60 Hz, 40 VA

2 µL min. (pathlength: 0.7 mm)

Ambient temperature, humidity

18 to 28°C, 40 to 80% (without dew condensation)

Light source

Xenon flash lamp

Dimensions & Weight

Width 210 mm x Depth 214 mm x Height 417 mm

Monochrometer

Holographic grating

7 kg*Quantitation range is a range which can secure the accuracy (±10% as a guide)
specified by us.

Software Specifications
Item

Specification

Item

Analysis mode

Simple nucleic acid quantitation, labeled nucleic acid quantitation,Analysis results display Detailed view (displays the focused sample analysis results and spectrum)

protein quantitation, labeled protein quantitation, photometric measurement
Simple nucleic acid quantitation Nucleic acid concentration (RNA, dsDNA, ssDNA, OligoDNA) calculation,Specification
List view (displays analysis results of all samples)

Analysis data judgment OD ratio (OD260 / 280, OD260 / 230), OD 800 judgment
PDF output

Analysis results (detailed view, list view) saved in PDF file

CSV output

Analysis results (detailed view, list view), spectra data saved in tab delimited text

nucleotide concentration calculation, Label concentration, labeling ratio calculation,User management

Multiple user or single user mode selected at the time of installation

OD ratio (OD260 / 280) calculation(Multiple user mode) User management: User registration, edit, deletion

OD ratio (OD260 / 280, OD260 / 230) calculation
Labeled nucleic acid quantitation Nucleic acid concentration (RNA, dsDNA, ssDNA, OligoDNA),

Protein quantitation

Protein concentration (M, µg/mL), label concentration, labeling ratio

Login: User name, password entry

Photometric measurement

OD display for 8 wavelengths maximum

Data saved in respective folders

Label management

Label registration (up to 8 new labels), edit, deletion
Default labels (Cy 3, Cy 5, Alexa Fluor 546, Alexa Fluor 647)

PC Requirements for BioSpec-nano
Item

Specification

OS

Windows 7 Professional 32/64bit edition* A separate PC is required.

1.6 GHz or higher processor

System memory

512 MB or larger (1 GB or larger when using Windows Vista)

Hard disk capacity

40 GB or larger

Display resolution

1024 × 768 pixels or above

USB 2.0 port

1 port or more (for connecting the instrument),

H: 417 mm

Windows 10 Pro 64bit edition
CPU2 ports or more when using a USB printerPrinter

Printer compatible with the system used

Disk drive

DVD-ROM or CD-ROM drive

Consumables
P/N

Part Name

206-25901

Wiping paper (highly absorbent wiper, 100 sheets/set)

W: 210 mm

D: 214 mm

BioSpec-nanoSpectrophotometer for Life Science11

BioSpec-nano

For Research Use Only. Not for use in diagnostic procedures.
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First Edition: December 2008, Printed in Japan 3655-08711-10AIT