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Pipette Service Done Right
Eppendorf Pipette Calibration

2 Eppendorf Service»Are Your Results Reliable?«
Premium services performed by experts ensure global
quality standards
Your pipettes are high-precision instruments that require
regular maintenance and inspection to stay in peak
performance. The precision and accuracy of your pipettes
are especially important to ensuring the quality of yourdownstream results. When your pipettes are not working
optimally, your experiment’s results might be questionable.
Regular maintenance, calibration and adjustment servicesperformed by Eppendorf experts can help you identify
potential issues and ensure your pipettes and dispensers
continue to generate reproducible results. Our global pipetteservices always adhere to the most recent, strict international
quality standards for calibration. Calibration options include
a thorough internal and external check of the pipette toensure wearable parts like O-rings are replaced in a timely
manner.

Volume Drift Due to Wear and Tear
From month to month over a yearlong testing period, a new
pipette in standard lab use calibrates within the error limits
shown in the graph below. But the pipette is also experiencing wear and tear, which can also be seen in the graph below
as the continuous trend of the pipette’s volume toward theupper limit. Without carrying out any maintenance, calibration or adjustment measures, this pipette will eventually
fall out of calibration, potentially causing unreliable or questionable results.

Systematic error in %Example of a volume drift over time

Eppendorf Service 3

What Else is Important?
Calibrating with 3 volumes to compensate for the non-linear correlation of error and volumeISO 8655 requires three volumes to be tested during calibration:
Nominal volume, 50% of nominal volume, and the lower limitof usable volume or 10% of nominal volume (whichever isgreater). Since the error limits of a pipette are not linear
from minimum to maximum volumes, it is possible that the10% and 100% volumes perform within the systematic error
limits while the 50% volume does not.

The graph above demonstrates this phenomenon: The blueline represents a pipette that is out of tolerance. The user
would not know this pipette is not functioning properly without testing all three volumes.

Calibrating with 10 measurements versus 4 measurements to compensate for deviations

994 µL996 µL994 µL991 µL996 µL995 µL996 µL995 µL994 µL993 µL995 µL993 µL992 µL992 µL

Four measurements – average 994.46 µL, -0.55% systematic error

Ten measurements – average 993.77 µL, -0.62% systematic error

The images above represent a set of calibration measurements from a 1,000 μL pipette. The image on the left showsthat taking only four measurements produces an error of-0.55%. As this is within the manufacturer's systematic error
limit of ±0.6%, the pipette would pass calibration.
On the right, taking all ten measurements producesa systematic error of -0.62%. As this is outside the manufacturer's systematic error limit of ±0.6%, the pipette would failcalibration. More confidence in the calibration is produced
and the results become statistically more accurate when using
ten measurements as recommended by ISO 8655.

4 Eppendorf Service

Why Select Eppendorf for Pipette Services?

Above and beyond ISO conformity

Highly trained service technicians

Thorough maintenance

We maintain over 5,000 pipettemanufacturer specifications
and conform to manufacturers’
standards when their specifications are stricter than ISO 8655
standards if requested by customer.

Our highly qualified technicians
complete 200 hours of training
and must pass rigorous exams toreceive certification before they
can begin servicing pipettes.

Our calibration services include
a thorough internal and external
check of the pipette to ensure
wearable parts like O-rings arereplaced in a timely manner.

Fully accredited Service Centers

Over 60 years of experience

Audit-proof documentation

In our major markets, ISO 17025accredited pipette calibration
processes are available both in thefield or in an Eppendorf ServiceCenter. As an expert partner inliquid handling, we can help you
meet any regulatory need.

Since we released the first piston
stroke pipette in 1961, we
continue to manufacture andservice pipettes with a passion forimproving user results.

Our calibration documentation
gives you peace of mind by
reducing the workload for yourqualification and regulatory audit
process. Most of our calibration
facilities worldwide use softwarethat complies with CFR21, part 11.

Eppendorf Service 5

Eppendorf Calibration Process
Decontamination
After verifying the customer’s declaration of decontamination,
Eppendorf sends all pipettes through an additional decontamination process before forwarding them to our calibration facilities.

Preventive Maintenance and Repair> Pipette is examined and calibrated as found, if requested> Outer and inner parts are cleaned and maintained (replace wearand-tear parts and lubricate, if necessary)> Device is tested for leaks> Performing necessary repairs

Calibration and Adjustment> High-precision, single- and multichannel balances fulfilling
ISO 8655 requirements are used
> Original manufacturer pipette tips are used
> Measurements made according to ISO 8655
> Evaluations carried out according to manufacturer’s specifications(if requested, on the basis of ISO 8655 or customer SOP error limits)> Adjustments made if device is outside of its error limits> ISO 17025 calibration certificate available on request.

Labeling and packaging> Device is packed with calibration documentation and service
report ready for shipment> Each device receives a calibration service sticker with thecalibration date
> Pipette shipped in secure packaging with our certified shipping
partners to assure it arrives in peak condition.

6 Eppendorf Service

Frequently Asked Questions
How often should you calibrate a pipette?
> You decide on the frequency of calibration within the scope of your quality standards. The frequency of calibration may vary depending on thenumber of users, the frequency of pipette use, the type of liquids dispensed and accepted tolerances. The recommendation is to carry out therenewal of conformity according to ISO 8655 at least once per year.

What is renewal of conformity?
> Calibration/renewal of conformity consists of pipette maintenance and as-left calibration. These services strictly adhere to ISO 8655 criteria,
ensuring the pipette meets conformity to ISO 8655 requirements or even stricter specifications like manufacturers’ specifications.

What happens, if my instrument cannot be repaired?
> We will inform you and offer you an attractive trade-in option.
> On request, we will dispose of your old instrument or send it back to you.

What is an ISO 17025 calibration certificate?
> A calibration certificate compliant with ISO 17025 not only contains the data from the calibration protocol, but also includes the measurement
uncertainty and the accreditation confirmation of the service center. The ISO 17025 calibration certificate along with the raw data ensure
traceability for the calibration.

Why do I need calibration without also including preventive maintenance?
> The as-is protocol is performed before the as-left calibration or renewal of conformity including maintenance. This protocol shows thecalibration status of the pipette as received from the user and indicates the pipette’s performance prior to renewal of conformity.

> Watch video:www.eppendorf.group/pipette-video

Eppendorf Service 7

Worldwide Pipette Calibration Services

 ppendorf Service Organization
E(ISO 17025 accredited pipette service facilities

Eppendorf-trained and certified Service Provider

Eppendorf offers pipette services around the globe throughits ISO 17025-accredited corporate service centers andEppendorf-trained and authorized service providers.

Eppendorf pipette services are standardized to ensure
your pipetting results are consistent and reproducible nomatter where in the world you are located.

Service Center or On-Site Services

Please contact your local Eppendorf Service Center for more information about our pipette services, which range fromcost-effective calibration to calibration services carried out in facilities accredited according to ISO 17025 and located in
our major markets. For fast return times, Eppendorf offers on-site and pick-up services in many countries.

Pipette Calibration Service
Calibration services: ordering information
ServiceCalibration
measurements»As is«Calibration
measurements»As left«

ISO 8655
10 per test volume1
without
Preventive
MaintenanceISO 8655
10 per test volume1
including
Preventive
Maintenance
3×4
4 per test volume1
without
Preventive
Maintenance
3×4
4 per test volume1
including
Preventive
Maintenance

External
decontamination2

Checking,
cleaning, lubricating4 before
calibration

includedincludedincludedincludedincludedincludedSingle channel 8-channel
pipette/pipettedispenser

12-channel
pipette16-channel
pipette24-channel
pipette0082 000 1400082 000 161 0082 000 162 0082 000 181 0082 000 182

0082 000 142

0082 000 165 0082 000 166 0082 000 185 0082 000 1860082 000 139

0082 000 159 0082 000 160 0082 000 179 0082 000 1800082 000 1410082 000 163 0082 000 164 0082 000 183 0082 000 184

Single channel
pipette/dispenser

8-channel
pipette0082 000 234

0082 000 235 0082 000 236 0082 000 237 0082 000 238

Calibration service packages: ordering information
Service

Calibration
measurements»As is«Calibration
measurements»As left«

ISO 8655
Verified

4 per test volume1

10 per test volume1 included

ISO 17025
including
Preventive
MaintenanceISO 17025
4 per test volume1
Advanced

10 per test volume1 included

included

0082 000 1430082 000 167 0082 000 168 0082 000 187 0082 000 188

10 per test volume1 included

included30082 000 227

0082 000 228 0082 000 229 0082 000 230 0082 000 231

10 per test volume1 10 per test volume1 included

included

0082 000 220

0082 000 221 0082 000 222 0082 000 223 0082 000 224

ISO 17025
Professional

External decontami- ISO 17025
accreditation
nation²; checking,cleaning, lubrication4 calibration

Additional services: ordering information

12-channel
pipette16-channel
pipette24-channel
pipetteAdditional services charges

ISO 17025 Calibration Certificate

0082 000 211

On-site calibration services in your own laboratoryinquireAdvanced Documentation (Excel file with calibration
information on all pipettes/dispensers per order)

0082 000 212

Labor Charge, 15 min. of repair services(time unit for one technician)0082 000 210

10%, 50% and 100% of nominal volumes for variable volume instruments.
² Pipettes and dispensers and package equipment need to be decontaminated before sending in.
³ Calibration certificate that is compliant with ISO 17025 accreditation is included with the second calibration
after maintenance is completed.
⁴ Internal parts

1

Service may differ by country.
Download decontamination form at www.eppendorf.com/decontamination.

We ensure our stringent, validated procedures are followed for Eppendorf and other brandname pipettes:

Qualification andCertification

ISO 8655
ISO 8655 defines the international standard criteria by which apipette (piston-operated volumetric apparatus) should be calibrated
and which maximum permissible error limits should be used ateach volume. ISO 8655 criteria include measurements at threevolumes, 10 measurements at each volume, tip exchange during
replicate measurement, controls for room environment and atest report without extended uncertainty of measurement. OurISO 8655-6 calibration services include a calibration certificate withextended uncertainty of measurement; for calibrations according toISO 8655-7, we provide a test report without extended uncertainty.

ISO 17025
Accreditation according to ISO 17025 ensures a high level ofcompetence and compliance with strict quality rules. ISO 17025
criteria include a traceability chain for measurement equipment,
measurement uncertainty values and approved quality standards.
Our ISO 17025 calibration services include certificates with all
raw data and extended measurement uncertainties.

Your local distributor: www.eppendorf.com/contact
Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com

www.eppendorf.com/service
Eppendorf®, the Eppendorf Brand Design, Eppendorf Reference®, and the epServices® logo are registered trademarks of Eppendorf SE, Hamburg, Germany.
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