MASS SPECTROMETRY

Benchtop Quadrupole-Orbitrap Mass Spectrometer

P rod u ct S p ecif ica t ions

Thermo Scientific
Q Exactive

Identify, quantify and confirm with unmatched
confidence using the Q Exactive massspectrometer.
• Resolving power up to 140,000• Maximum scan speed 12 Hz• Intra-scan dynamic range > 5000:1• Quadrupole mass filter• Spectral multiplexing for enhanced duty cycle• S-Lens ion source for enhanced sensitivity

The Q Exactive benchtop LC-MS/MS
combines high-performance quadrupole
precursor selection with high-resolution,accurate-mass (HR/AM) Orbitrap detection
to deliver high performance and tremendous
versatility.
With a fast scan speed and multiplexing
capabilities, the Q Exactive mass spectrometer is an outstanding detector for fast
chromatography separation techniques. The
superior quality of Q Exactive MS/MS data
enables identification and quantitation of
more compounds with greater confidence.

Thermo Scientific Q Exactive MS system.

The Q Exactive LC-MS/MS system not onlyoffers broad screening capabilities but also
excels at targeted quantitation experiments,
making it a versatile addition to any laboratory. Fast, alternating positive-negative scanmodes allow for the most comprehensive
approaches, saving time during screening
experiments. The benchtop system is ideallysuited for drug metabolism, proteomics,environmental analysis, food safety andclinical research applications.

2Hardware Specifications
Thermo Scientific Ion Max API Source• Enhanced sensitivity and ruggedness• Sweep Gas reduces chemical noise• 60° interchangeable ion probe orientation• Removable metal ion transfer capillaryprovides vent-free maintenanceIon Source• H-ESI II probe with Dual Desolvation Zone
technology equipped as standard• A progressively spaced stacked ring ionguide (S-lens)• The S-lens is a radio frequency (RF)device that captures and efficiently
focuses the ions in a tight beam
• Large variable spacing between electrodes
allows for better pumping efficiency andimproves ruggedness
Transfer Ion Optics• Advanced ion guides for high sensitivity
and ruggedness• High stability and ion transmission
efficiency

Quadrupole Mass Filter• Thermo Scientific HyperQuad mass filter
provides superior and unique combinationof resolution and sensitivity• Variable precursor isolation width
selection from 0.4 Da to full mass range
Vacuum System• Differentially pumped vacuum system withfinal vacuum < 1 × 10-9 mbar
• Two split-flow turbomolecular pumps andone rotary vane pump

Performance Characteristics
Resolving power

Up to 140,000 @ m/z 200

Mass range

50 to 6,000 m/z

Scan rate*

Up to 12 Hz at resolution
setting of 17,500@ m/z 200

Mass accuracy * Internal: < 1 ppm RMS
External: < 3 ppm RMS
Sensitivity

Full MS: 500 fg Buspirone
on column S/N 100:1
SIM: 50 fg Buspirone
on column S/N 100:1Dynamic range> 5000:1• Seven vacuum regions
Orbitrap Mass Analyzer• Gas (nitrogen) filled C-Trap• Highly efficient ion transfer to Orbitrap
analyzer• Straight multipole collision cell for HCD• Orbitrap analyzer with 5 kV central
electrode voltage• Low-noise image current pre-amplifier• 16-bit signal digitalization
Data Acquisition• Ultra fast real-time data acquisition andinstrument control system• Fully automated calibration via instrument
control software• Automated gain control

Polarity switching One full cycle in < 1 sec(one full scan positivemode and one full scan
negative mode at 		resolution setting of 		35,000)
Multiplexityup to 10 precursors/scan

Analog inputs

One (1) analog input(0 – 1 V)
One (1) analog (0 – 10 V)* Under defined conditions

Options• Nanospray source supports static packed
tip and dynamic nanospray experiments,compatible with liquid flow rates of 50 nL/
min to 2 μL/min• NanoSpray Flex Ion source – single set-up
for all online nanoflow applications• ESI probe compatible with liquid flow
rates of < 1 μL/min to 1 mL/min without
splitting• APCI source compatible with liquid flow
rates of 50 μL/min to 2 mL/min withoutsplitting• APCI/APPI source compatible with liquidflow rates of 50 μL/min to 2 mL/min
without splitting• Metal needle kits for high and low flow
analyses

Schematic Schematic of the Thermo Scientific Q Exactive Benchtop LC-MS/MS.

Software Features
Data System• High performance PC with Intel®
Pentium® microprocessor• High resolution LCD color monitor• Microsoft Windows® 7 operating system• Microsoft Office software package• Thermo Scientific Xcalibur processing andinstrument control software• New workflow based method editor
Operation Modes• Full MS with high resolution accuratemass detection• Selected Ion Monitoring (SIM) with highresolution accurate mass detection• Parallel reaction monitoring (PRM) for
improved screening and quantitative
confidence• Data-Idependent Acquisition (DIA) for
unbiased identification and quantitative
analysis• MS/MS of isolated ions with highresolution accurate mass detection• In-source fragmentation of all ions in thesource region• Positive/negative ion switching on
chromatographic timescale• Data Dependent on-the-fly decision
making• Timed SIM for scheduled data acquisitionof the targets of interest
Exclusive Technologies• Unique, patented* Thermo Scientific
Automatic Gain Control (AGC) ensuresthat the Orbitrap is always filled with theoptimum number of ions for all scans• High performance HCD collision cellfor highest performance MS/MSfragmentation• Collision energy profiling using different
collision energies for HCD fragmentation• Advanced signal processing• Interleaved operation• Multiplex-MS for simultaneous detectionof up to 10 precursor ions in the Orbitrap
mass analyzer• All Ion Fragmentation (AIF) in the HCD
collision cell with high resolution accuratemass detection

The Thermo Scientific Q Exactive™ mass spectrometer with the Thermo Scientific Ultimate 3000 RSLC.

3Specifications
P rod u ct S p ecif ica t ions

Installation Requirements
Power2× 230 Vac ± 10% single phase, 15 Ampere, 50/60 Hz, with earth ground for the instrument
120 or 230 Vac single phase with earth ground for the data system
Gas
NitrogenHigh purity nitrogen gas supply (99% pure at 800 ± 30 kPa (8.0 ± 0.3 bar, 116 ± 4 psi))
Environment
System averages 2,500 W (~9,000 Btu/h) output when considering air conditioning needs
Operating environment must be 15 - 26 °C (59 - 78 °F) and relative humidity must be
40–70% with no condensation
WeightQ Exactive mass spectrometer: 182 kg (401 pounds) without forevacuum pump
Forevacuum pump: 62 kg (136 pounds)
Dimensions
Q Exactive mass spectrometer: (h × d × w)
95 × 83 × 91 cm(37 × 33 × 36 inches)

91 cm(36 in.)

95 cm(37 in.)www.thermofisher.com/QExactive©2016 Thermo Fisher Scientific Inc. All rights reserved. Intel and Pentium are registered trademarks of Intel Corporation; Microsoft andWindows are trademarksof the Microsoft group of companies. All other trademarks are the property of Thermo Fisher Scientific and its
subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local
sales representative for details.

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