Compact DryTM LS Listeria test dish Listeria colonies appear blue/light blue Culture conditions: culture at 35 ° C ± 1 ° C or 37 ° C ± 1 ° C for 24 h ± 2 h

Main ingredients:

Chromogenic medium containing selective reagent

Applicable samples:

Environment and food

Storage conditions:

Room temperature (1-30 ° C)

Shelf life:

18 months

Strengths

Compact Dry LS 40 pieces / box Code 54060-40
Compact Dry LS 240 pieces / box Code 54060-240
Swab 1ml Smear Stick 200 / box Code 06738
EZ Reach Sponge Stick with Handle100pcs/carton Code EZ-10HC-PUR

Compact Dry test dish is produced using
the unique patented technology of SDC

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Compact DryTM LSListeria test dishInterpretation
manualCom pact DryTM LS Listeria test dish of Nissin is a pre-prepared medium containing selective
reagents, nutrients, and color indicator. It can be used to detect Listeria monocytogenes and also
can be used for the detection of Listeria in products. The species of Listeria to be tested include: L.
monocyto-genes, L.grayi, L.welshimeri, L.ivanovii, L.innocua.
Environmental conditions and disinfectants have the potential to inhibit and damage
microorganisms. The recommended Swab smudge stick and EZ Reach smear sponge from Nissuicontain microbe-repairing ingredients without the need for additional BPW. The repair process ofListeria in the smear stick and smear sponge is not an enrichment step.

1. Typical colony of Listeria, blue/light blue colonies
Qualitative interpretation: Listeria is detected.
Semi-quantitative interpretation: recorded as aform of guidance for sampling areas and corporate
standards. (such as low, medium, high, or acceptableand unacceptable).
Quantitative interpretation: Number of Listeria on
the test dish: 18.
Please refer to the “Quantitative Sampling andInterpretation” section to calculate the number ofListeria in each sample.

2. The test dish is cultured for 24 hours without colony
growth.
Qualitative interpretation: Listeria is not detected.
Semi-quantitative interpretation: recorded as a form ofguidance for sampling areas and corporate
standards. (such as low, medium, high, or acceptableand unacceptable).
Quantitative interpretation: Number of Listeria on
the test dish: <1.
Please refer to the section “Quantitative Sampling andInterpretation” to calculate the number of Listeria ineach sample.

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Compact DryTM LS
Listeria test dish3. The CompactDry LS Listeria test dish is
selective for Listeria and the colony is shown inblue/light blue.
Qualitative interpretation: Listeria is detected.
Semi-quantitative interpretation: recorded as aform of guidance for sampling areas andcorporate standards. (such as low, medium, high,or acceptable and unacceptable).
Quantitative interpretation: Number of Listeriaon the test dish: 72.
Please refer to the section “QuantitativeSampling and Interpretation” to calculate thenumber of Listeria in each sample.

4. When there are many colonies, there may be
many small, unclear, or all blue colonies on thetest dish.
Qualitative interpretation: Listeria is detected.
Semi-quantitative interpretation: recorded as aform of guidance for sampling areas andcorporate standards. (such as low, medium, high,or acceptable and unacceptable).
Quantitative interpretation: The number ofListeria monocytogenes on the test dish is Too
Numerous To Count (TNTC).

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Compact DryTM LS
Listeria test dishInstructions

Listeria detection in the environment
Sample Preparation

Collect environmental samples with sampling equipment such as smear stick and smear sponge.
A. Sampling method with smear stick

Open Swab's yellow cap with aSmear the area to be inspected toPut the cotton swab back into thecotton swab

10*10cm, apply appropriate force,and pay attention to the continuous
rotation of smear stick

container, tighten the cap andshake it properly. The Swab isplaced at room temperature(20-30 ° C) for 1 h, and thelongest time is not more than 1.5
h to repair the damaged Listeria.

B. Sampling method with smudge sponge

Peel the top of the sampling bag
along the indicator line, hold theplastic handle line above the hand,and remove the smear sponge.

The area to be inspected is30*30cm, and the handle ispressed and bent to ensure thatthe sponge head is in full contact
with the sampling surface andPut the smear sponge back into
the sampling bag and rotate thehandle counterclockwise to drop
the sponge head into thesampling bag and seal it. Store atnote that the tip of the sponge issmeared to the same position.

room temperature (20-30 ° C) for
1 h, and the longest time is not
more than 1.5 h to repair thedamaged Listeria.

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Compact DryTM LS
Listeria test dish

Vaccination culture

Swab smear stick

EZ Reach smear sponge

After proper shaking, open the port
screw cap and drop 1ml sample into
the LS test dish.

Extrusion of the liquid in the sponge,
pipetting 1 ml of the sample solution, anddropping it vertically into the LS test dish.

Inverted into the incubator at 35 ° C ±1 ° C or 37 ° C ± 1 ° C for 24 h ± 2 h

Interpretation of Listeria. Pour it on a whitebackground or translucent plate, and count itwith a visual and colony counter.

Environmental sampling tool
Swab Smear Stick

EZ Reach Sponge smear stickwith handle

The recommended smearing
area for 1ml BPW buffer solution

The recommended smearing

is 10*10cm

area for 10ml Hicap buffer
solution is 30*30cm

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Compact DryTM LS
Listeria test dish

Listeria detection in foodSample Preparation

Sample dilutions of 1:10 or greater
dilution factor are prepared. Weigh
25g of food sample, place in asuitable sterile container.

Add 225 ml of BPW sterile
dilution.

Stir or homogenize the sample.
Store at room temperature(20-30 ° C) for 1 h, and thelongest time is not more than 1.5
h to repair the damaged Listeria.

nverted into the incubator at35 ° C ± 1 °C or 37 ° C ± 1 °C
for 24 h ± 2 h.

Interpretation of Listeria. Pourit on a white background ortranslucent plate, and count itwith a visual and colony counter.

Vaccination culture

Pipetting 1 ml of the sample
solution, and dropping it verticallyinto the LS test dish.

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Compact DryTM LS
Listeria test dishCompactDry LS Listeria Test Method can be used for qualitative, semi-quantitativeand quantitative detection

Qualitative detection
The results are counted as detected and notdetected based on the presence or absence ofblue/light blue colonies.
If the result of yes/no is sufficient to meet thereporting requirements, then you can choose
qualitative testing.

Not detected

Detected

Semi-quantitative
The results are recorded based on the relative
number of blue/light blue colonies.
If you are taking the appropriate measures
based on the relative number of colonies andthere is no need to record the actual number ofcolonies, you can choose semi-quantitative
testing.

The assay of Listeria should be recorded as a guideline
for sampling areas and corporate standards. (such as
low, medium, high, or acceptable and unacceptable)

Quantitative detection
Count all blue/light blue colonies.
If you take the appropriate action based on theactual number of colonies, you can use
quantitative testing.

The number of Listeria on the test dish is 12
Please refer to the “Quantitative Sampling andInterpretation” section on the next page to calculate thenumber of Listeria in each sample.

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Compact DryTM LS
Listeria test dish

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Compact DryTM LS
Coliform bacteria test dishQuantitative sampling and interpretation
The number of colonies per unit area is calculated in a quantitative manner using a CompactDry LS Listeria
test dish.
Sampling stability:Use the same type of sampling tool, template area, technician and sampling technique.
Sampling sensitivity: Sampling area: The size can be set according to regulations, internal standards, and monitoring
locations.
Sampling tools: The volume of the wetting fluid and the ratio of the inoculum amount affect the dilution
factor.

In order to determine Listeria in a unit sampling area, the following data needs to be recorded:
1. Sampling area
2. The amount of liquid in the wet sampling device
3.
4.

Inoculation volume
Number of colonies on the test dishCalculate the number of colonies per unit sampling area using the following formula.
CFU/area=number of colonies* (ml of moisturizing solution) sampling area
Example: Sponge sample method

Wet the sponge with 10
ml of liquid and smear
the sampling surface,

Put the smear spongeback into the sampling
bag and repair thePipette 1ml of sample
solution and add it tothe LS Listeria test

After cultivation, countcolonies(This example
assumes that the number

such as 1 square foot(1ft2), about 30*30 cm.

damaged Listeria directlyat room temperature.

dishof colonies is 30)

A. Wet fluid volume mL --------------------------------10
B. Inoculum size mL ------------------------------------- 1
C. A divided by B ----------------------------------------10
D. Number of colonies on the test dish -------- 30
E. C multiplied by D ---------------------------------- 300
F. Sampling area --------------------------------------1ft2
G. E divided by F -------------------------- 300CFU/1ft2

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