Technical Bulletin

Thermoacidophilic bacterial (TAB) spore removal
by 0.45 µm membrane cartridge filters
Overview

Supporting Data

Spore-forming microbes, such as Alicyclobacillus
species, negatively affect product quality andprovide risks to manufacturers of fruit juice andingredients utilized in beverage production(e.g. sugars, flavors and colors). Alicyclobacillus
species, though nonpathogenic and not a risk
for consumption, do cause economic damage.

A study of A. acidoterrestris spore removal (bacteriain the TAB spoilage group) shows that the Supor®
membrane from Oenopure (GB grade) andMEMBRAcart XL II (A045 grade) filters provides
complete retention of the TAB spores in different
fluids tested under laboratory conditions. In thissame testing it was shown that the Supor
membrane from MEMBRAcart XLII (A065 grade)
filters retained spores completely whilst only theSupor membrane 0.8 μm membrane showed slight
passages of spores. The challenge test results aresummarized in Table 1.

The membrane capability study detailed below
provides the rationale by which Oenopure™ (GBgrade) and MEMBRAcart XL II (0.45 micron grade)should be utilized for this application.

Table 1: Results of TAB Spore Challenge Tests
Membrane fromfilter type

Challenge Fluid

LRV*

Oenopure GBW andMEMBRAcart XLII 419A045

1/15mol phosphate buffer>7.8, >7.8, >7.8

100% clear apple juice>7.0, >7.0, >7.0

High fructose corn syrup>7.8, >7.3, >7.3

1/15mol phosphate buffer>7.3

100% clear apple juice> 6.4 and > 6.5

High fructose corn syrup>6.8 and >7.3

Supor EBW Control

Oenopure GKW andMEMBRAcart XLII 419A065

Supor 800

1/15mol phosphate buffer>7.9, >7.9, >7.9

100% clear apple juice>7.0, >7.0, >7.0

High fructose corn syrup>7.8, >7.8, 7.8

1/15mol phosphate buffer

6.6 and 6.7

100% clear apple juice

5.3 and 5.7

High fructose corn syrup

5.7 and 6.3* Data shown in the table represent the log reduction value (LRV) of three filter membrane diskswith TAB spores. The total challenge was at least 10 6 up to more than 10 7 CFU per filter disk.

Recommendation
Oenopure (GB grade) and MEMBRAcart XLII (419A045) are recommended
for use in applications for TAB spore removal.

Pall Food and Beverage

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Pall Corporation has offices and plants throughout the world. For Pall representatives
in your area, please go to www.pall.com/contact

foodandbeverage@pall.com

Please contact Pall Corporation to verify that the product conforms to your national
legislation and/or regional regulatory requirements for water and food contact use.
Because of technological developments related to the products, systems, and/orservices described herein, the data and procedures are subject to change without notice.
Please consult your Pall representative or visit www.pall.com to verify that thisinformation remains valid.
© Copyright 2014, Pall Corporation. Pall,, Oenopure, and Supor are trademarks of PallCorporation. ® Indicates a trademark registered in the USA. Filtration. Separation. Solution.SM andBETTER LIVES. BETTER PLANET. are service marks of Pall Corporation.

FBTBTABENa

March 2014