Plant
No

SquashCharacters

104(08003)
No. of samples

Methods

Primary essential character
Rank or measurement unit

Remarks

to the 1st decimal place)

Length of dried ripe seeds

1

Seed length

10 seeds

Measurement

mm (round2

Color of seed coat

10 seeds

Observation

0:No seed coat 1:White 2:Milky white or graywhite 3:Light yellow 4:Light yellowish brown
5:Yellowish brown 9:Black

Color of seed coat of dried ripe seeds

3

Size of cotyledon

5 plants

Measurement

cm (round

Width of fully expanded cotyledons

4

Plant type5 plants

Observation

1:Dwarf

to the 1st decimal place)
2:Intermediate

3:Climbing

At the time of the first fruit setting or 3
months after sowing

5

Shape of stem

5 plants

Observation

3:Round

5:Intermediate

7:Pentagonal

Shape of cross section at the 5th-10th nodes onstem which sets the first fruit or on thestrongest stem at 3 months after sowing

6

Leaf size

5 plants

Observation

Size of the 6th-10th leaf on stem which sets
1:Extremely small 2:Very small 3:Small
4:Slightly small 5:Intermediate 6:Slightly
the first fruit or on the strongest stem at 3large 7:Large 8:Very large 9:Extremely large
months after sowing

7

Depth of sinus of

5 plants

Observation

0:Absent 1:Extremely shallow 2:Very shallow
3:Shallow 4:Slightly shallow 5:Intermediate
6:Slightly deep 7:Deep 8:Very deep
9:Extremely deep

Depth of sinus of the 6th-10th leaf on stem

0:Absent 1:Almost none 2:Extremely few
3:Very few 4:Few 5:Intermediate 6:Some
7:Many 8:Very many 9:Extremely numerous

Quantity of spots or checks of the 6th-10th

leaves8

Leaf spots

5 plants

Observation

which sets the first fruit or on the strongest
stem at 3 months after sowing

leaf on stem which sets the first fruit or on
the strongest stem at 3 months after sowing

9

Length of petiole

5 plants

Observation

1:Extremely short 2:Very short 3:Short
4:Slightly short 5:Intermediate 6:Slightly
long 7:Long 8:Very long 9:Extremely long

Length of petiole of the 6th-10th leaf on stem
which sets the first fruit or on the strongest
stem at 3 months after sowing

10

First pistillate flower 5 plants
bearing nodeObservation

0:Not bearing 1:Extremely low 2:Very low
3:Low 4:Slightly low 5:Intermediate
6:Slightly high 7:High 8:Very high
9:Extremely high

Average order of node which bears the firstfemale or bisexual flower

PlantNo

SquashCharacters

104(08003)
No. of samples

Methods

Primary essential character
Rank or measurement unit

11

Sex type

5 plants

Observation

1:Androecious 2:Monoecious 3:Hermaphroditic
and monoecious 4:Andromonoecious
5:Gynomonoecious 6:Gynoecious
7:Hermaphroditic 8:Male sterile 9:Female
sterile12

Fruit shape (1)5 plants, 5 fruits Observation

1:Globular 2:Flattened 3:Disk 4:Cylindrical
5:Oval 6:Heart shaped 7:Pyriform 8:Dumbbell
9:Other

Remarks

Shape of fully ripe fruit. If none of theseranks apply, choose '9' and select from
'No.13'.

13

Fruit shape (2)5 plants, 5 fruits Observation

1:Elongate 2:Elongate and curved 3:Crookedneck 4:Turbinate superior 5:Turbinate
inferior 6:Crowned 7:Other

Shape of fully ripe fruit

14

Fruit pleat

5 plants, 5 fruits Observation

0:Absent 1:Extremely shallow 2:Very shallow
3:Shallow 4:Slightly shallow 5:Intermediate
6:Slightly deep 7:Deep 8:Very deep
9:Extremely deep

Fruit pleat of fully ripe fruit

15

Predominant fruit skin

5 plants, 5 fruits Observation

1:White5:GreenPredominant color is the color covering thecolor

2:Gray 3:Yellow 4:Gray green
6:Orange 7:Red 8:Brown 9:Blacklargest surface area of the fully ripe fruit.
In case two colors have the same area, thelighter color is considered to be thepredominant one.

16

Secondary fruit skin

5 plants, 5 fruits Observation

color

0:Absent 1:White 2:Gray 3:Yellow 4:Graygreen 5:Green 6:Orange 7:Red 8:Brown
9:Black

Secondary color is the color covering thesecond largest area of the fully ripe fruit. In
case two colors have the same area, the darker
is considered to be the secondary one.

17

Fruit weight

5 plants, 5 fruits Measurement

kg (round18

Flesh color

5 plants, 5 fruits Observation

1:White 2:White green 3:Light yellow
4:Yellow 5:Deep yellow 6:Orange yellow
7:Orange 8:Peach 9:Other

Flesh color of fully ripe fruit

19

Shape of peduncle

5 plants, 5 fruits Observation

3:Round

Shape of peduncle of fully ripe fruit

to the 1st decimal place)

5:Medium

7:Pentagonal-octagonal

Weight of fully ripe fruit

PlantNo
20

SquashCharacters

Character of base ofpeduncle

104(08003)
No. of samples

Methods

5 plants, 5 fruits Observation

Primary essential character
Rank or measurement unit

Remarks

Hardness and shape of the part where the1:Soft cylindric 2:Soft coned 3:Soft fannedout 4:Slightly hard cylindric 5:Slightly hard peduncle attached to fruit
coned 6:Slightly hard fanned out 7:Hardcylindric 8:Hard coned 9:Hard fanned out

PlantSquash104(08003)

Primary optional character

No

Characters

1

Condition of seed coat

10 seeds

Observation

Condition of seed coat of dried mature seed
1:Extremely smooth 2:Very smooth 3:Smooth
4:Slightly smooth 5:Intermediate 6:Slightly
rough 7:Rough 8:Very rough 9:Extremely rough2

Shape of funiclus trace 10 seeds

Observation

1:Rounded
5:Pointed

10 seeds

Observation

0:Not thickened 1:Extremely thin 2:Very thin
3:Thin 4:Slightly thin 5:Intermediate
6:Slightly thick 7:Thick 8:Very thick
9:Extremely thick

Thickness of margin of dried ripe seed

10 seeds

Observation

3:Smooth 4:Slightly smooth 5:Intermediate
6:Slightly serrated 7:Serrated

Condition of margin of dried mature seed

No. of samples

Methods

of seed3

Thickness of seedmargin4

Condition of seedmarginRank or measurement unit

2:Polygonal

3:Slant

Remarks

4:LevelShape of funiculus trace of dried ripe seed

5

Color of seed margin

10 seeds

Observation

Color of margin of dried ripe seed
1:Gray white 2:Gray yellow 3:Gray yellowish
brown 4:Yellowish brown 5:Light brown
6:Silver green 7:Silver blue 8:Black 9:Other

6

Shape of cotyledon

5 plants

Observation

1:Rounded

3:Short oval

5:Oval7:Long oval

Observe at the time when cotyledon fully
expanded78

Color of cotyledon

Length of hypocotyl

5 plants

5 plants

Observation

Measurement

3:Light green 4:Slightly light green
6:Slightly dark green 7:Dark green
cm (round

5:Greento the 1st decimal place)Observe at the time when cotyledon fully
expanded
Measure at the time when cotyledon fully
expanded

9

Diameter of hypocotyl

5 plants

Measurement

mm (roundto the 1st decimal place)

Measure at the time when cotyledon fully
expanded

10

Plant vigor

5 plants

Observation

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highEvaluate at the time of the first set or 3
months after sowing

PlantSquashNo

Characters

11

Softness of stem

104(08003)
No. of samples
5 plants

Methods
Observation

Primary optional character
Rank or measurement unit

Remarks

1:Extremely soft 2:Very soft 3:Soft
4:Slightly soft 5:Intermediate 6:Slightly
hard 7:Hard 8:Very hard 9:Extremely hard

Softness of stem at 5th-10th nodes which sets
the first fruit or on the strongest stem at 3
months after sowing

12

Thickness of stem

5 plants

Observation

Diameter of stem at 5th-10th nodes which sets
1:Extremely thin 2:Very thin 3:Thin
4:Slightly thin 5:Intermediate 6:Slightly
the first fruit or on the strongest stem at 3
thick 7:Thick 8:Very thick 9:Extremely thickmonths after sowing

13

Internode length

5 plants

Measurement

cm (round

Average length of internode at 5th-10th node on

to the 1st decimal place)stem which sets the first fruit or on thestrongest stem at 3 months after sowing
14

Branching habit

5 plants

Observation

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highEvaluate based on the number of branches at thetime of the first fruit set or 3 months aftersowing15

Number of tendrils

5 plants

Observation

0:Absent 1:Almost none 2:Extremely few
3:Very few 4:Few 5:Intermediate 6:Some
7:Many 8:Very many 9:Extremely numerous

Observe at the time of first fruit setting

16

Leaf shape

5 plants

Observation

1:Ovoid 2:Slightly ovoid 3:Round 4:Slightly
round 5:Intermediate 6:Slightly angular
7:Angular

Shape of the 6th-10th leaf on stem which sets
the first fruit or on the strongest stem at 3
months after sowing

17

Condition of leaf

5 plants

Observation

margin3:Smooth 4:Slightly smooth 5:Intermediate
6:Slightly serrated 7:Serrated

Condition of margin of the 6th-10th leaf onstem which sets the first fruit or on thestrongest stem at 3 months after sowing

18

Spot color of leaf

5 plants

Observation

0:Absent 1:Light green 2:Silver
green and silver 4:Other

3:Light

Spot color of the 6th-10th leaf on stem whichsets the first fruit or on the strongest stemat 3 months after sowing

19

Texture of pubescenceon leaf

5 plants

Observation

0:Absentspiny

1:Soft hairy

2:Hard hairy

3:HardTexture of pubescence of the 6th-10th leaves onstem which sets the first fruit or on thestrongest stem at 3 months sowing

PlantSquashNo

Characters

20

Density of pubescence

104(08003)
No. of samples

Methods

Leaf color

Rank or measurement unit

Remarks

5 plants

Observation

0:Absent 1:Extremely thin 2:Very thin 3:Thin Density of pubescence of the 6th-10th leaf on
4:Slightly thin 5:Intermediate 6:Slightly
stem which sets the first fruit or on thedense 7:Dense 8:Very dense 9:Extremely dense
strongest stem at 3 months after sowing

5 plants

Observation

3:Light green 4:Slightly light green
6:Slightly dark green 7:Dark green

on leaf21

Primary optional character

5:GreenColor of the 6th-10th leaf on stem which sets
the first fruit or on the strongest stem at 3
months after sowing

22

Color of flower

5 plants

Observation

1:White2:Yellow

3:Orange

4:Other

Flower color at the time of the first fruit set
or 3 months after sowing

23

Time to first female

5 plants

Observation

Average days to the first female or bisexual
0:No female flower 1:Extremely short 2:Veryshort 3:Short 4:Slightly shortflowering
5:Intermediate 6:Slightly long 7:Long 8:Verylong 9:Extremely long

5 plants

Observation

Average days to the first male flowering
0:No male flower 1:Extremely short 2:Veryshort 3:Short 4:Slightly short
5:Intermediate 6:Slightly long 7:Long 8:Verylong 9:Extremely long

flowering

24

Time to first male
flowering

25

Fruit rib shape

5 plants, 5 fruits Observation

0:Absent

3:V-shaped

Fully ripe fruits

26

Shape of stem-end of

5 plants, 5 fruits Observation

1:Depressed 2:Slightly depressed 3:Flattened
4:Slightly rounded 5:Rounded 6:Slightly
pointed 7:Pointed

Fully ripe fruits

Shape of blossom-end of 5 plants, 5 fruits Observation

1:Depressed 2:Slightly depressed 3:Flattened
4:Slightly rounded 5:Rounded 6:Slightly
pointed 7:Pointed

Fully ripe fruits

5 plants, 5 fruits Observation

3:Light 4:Slightly light
6:Slightly dark 7:Dark

Fully ripe fruits

5 plants, 5 fruits Observation

0:No secondary color 1:Speckled 2:Spotted
3:Striped 4:Streaked 5:Bisectional 6:Other

fruit
27

fruit
28

Shade of predominantfruit skin color

29

Design produced bysecondary fruit skincolor

1:Round

2:Intermediate

5:Intermediate

Fully ripe fruits

PlantNo

SquashCharacters

104(08003)
No. of samples

Methods

Primary optional character
Rank or measurement unit

Remarks

30

Fruit skin texture

5 plants, 5 fruits Observation

1:Smooth 2:Grainy 3:Finely wrinkled
4:Shallowly wavy 5:Netted 6:With warts
7:With spines 8:Knoted 9:Other

Fully ripe fruits

31

Glossiness of fruit

5 plants, 5 fruits Observation

0:Absent 3:Weak 4:Slightly weak
5:Intermediate 6:Slightly prominent
7:Prominent

Fully ripe fruits

skin
32

Bloom on fruit skin

5 plants, 5 fruits Observation

0:Absent 1:Extremely weak 2:Very weak 3:Weak
4:Slightly weak 5:Intermediate 6:Slightly
prominent 7:Prominent 8:Very prominent
9:Extremely prominent

33

Size of blossom end

5 plants, 5 fruits Observation

Fully ripe fruits
1:Extremely small 2:Very small 3:Small
4:Slightly small 5:Intermediate 6:Slightly
large 7:Large 8:Very large 9:Extremely large

34

Height of fruit

5 plants, 5 fruits Measurement

cm (roundto the 1st decimal place)

Fully ripe fruits

35

Width of fruit

5 plants, 5 fruits Measurement

cm (roundto the 1st decimal place)

Fully ripe fruits

36

Hardness of fruit skin

5 plants, 5 fruits Observation

1:Extremely soft 2:Very soft 3:Soft
4:Slightly soft 5:Intermediate 6:Slightly
hard 7:Hard 8:Very hard 9:Extremely hard

Fully ripe fruits. Soft:easily marked byfingernail, intermediate:difficult to mark with
fingernail, hard:impossible to mark with
fingernail

37

Flesh thickness

5 plants, 5 fruits Observation

Evaluate based on the flesh thickness index
1:Extremely thin 2:Very thin 3:Thin
4:Slightly thin 5:Intermediate 6:Slightly(flesh thickness/radius of fruit x 100), 1:<
thick 7:Thick 8:Very thick 9:Extremely thick15, 2:16-22, 3:23-29, 4:30-36, 5:37-43, 6:4450, 7:51-57, 8:58-64, 9:> 65

38

Brightness of flesh

5 plants, 5 fruits Observation

3:Bright 4:Slightly bright
6:Slightly dark 7:Dark

5 plants, 5 fruits Measurement

cm (round

color
39

Length of peduncle

5:Intermediate

to the 1st decimal place)

Fully ripe fruit

Fully ripe fruit

PlantSquashNo

Characters

40

Thickness of peduncle

104(08003)
No. of samples

Methods

5 plants, 5 fruits Observation

Primary optional character
Rank or measurement unit

Remarks

Fully ripe fruit. The part of the center of
1:Extremely thin 2:Very thin 3:Thin
4:Slightly thin 5:Intermediate 6:Slightly
pedunclethick 7:Thick 8:Very thick 9:Extremely thickPlantSquashNo

Characters

1

Resistance to fusarium

104(08003)
No. of samples
5 plants

Methods
Observationwilt
2

Resistance to powdery

5 plants

Observation

mildew
3

Resistance to virus

5 plants

Observationdiseases
4

Resistance to

5 plants

Observation

Phytophthora rot
5

Earliness

5 plants

Observation

Secondary essential character
Rank or measurement unit

Remarks

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely high

Artificial inoculation for young seedling or

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely high

Artificial inoculation for young seedling or

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely high

Artificial inoculation for young seedling or

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely high

Artificial inoculation for young seedling or

1:Extremely early 2:Very early 3:Early
4:Slightly early 5:Intermediate 6:Slightly
late 7:Late 8:Very late 9:Extremely late

Date of harvesting half the plants investivatednatural infection in field

natural infection in field

natural infection in field

natural infection in fieldPlantNo
1

SquashCharacters

104(08003)
No. of samples

Resistance to bacterial 5 plants

Methods
Observation

spot
2

3

4

Resistance to insects

5 plants

Resistance to nematodes 5 plants

Tolerance to low

Observation

Observation

Tolerance to high

Excess water tolerance

Remarks

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely high

Artificial inoculation for young seedling or

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely high

Artificial inoculation for young seedling or

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely high

Artificial inoculation for young seedling or

natural infection in field

natural infection in field

natural infection in field

Observation

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highSeedling or field test

5 plants

Observation

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highSeedling or field test

5 plants

Observation

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highSeedling or field test

temperature
6

Rank or measurement unit

5 plants

temperature
5

Secondary optional character

PlantNo
1

SquashCharacters

Degree of continuous

104(08003)
No. of samples

Methods

Rank or measurement unit

Remarks

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highObserve at the end of harvesting time, exclude

1:Extremely short 2:Very short 3:Short
4:Slightly short 5:Intermediate 6:Slightly
long 7:Long 8:Very long 9:Extremely long

Average number of days from flowering to

5 plants, 5 fruits Observation

1:White5:GreenFruit at maturity for table use

5 plants, 5 fruits Observation

3:Light 4:Slightly light
6:Slightly dark 7:Dark

5 plants, 5 fruits Sensory

Bitterness of flesh at full or table use
0:Absent 1:Extremely low 2:Very low 3:Low
4:Slightly low 5:Intermediate 6:Slightly high maturity
7:High 8:Very high 9:Extremely high5 plants

Observation

female flowering
23Days to harvesting

Predominant fruit skin

Tertiary essential character

5 plants, 5 fruits Observation

color at maturity for

2:Gray 3:Yellow 4:Gray green
6:Orange 7:Red 8:Brown 9:Blackdwarf species

harvesttable use
4

Shade of predominantfruit skin color at

5:Intermediate

Fruit at maturity for table use

maturity for table use
5

Bitterness of flesh

PlantNo

SquashCharacters

104(08003)
No. of samples

Methods

Tertiary optional character
Rank or measurement unit

Remarks

1

Flesh texture

5 plants, 5 fruits Sensory

1:Fibrous 2:Spongy
5:Sticky 9:Other

2

Firmness of flesh

5 plants, 5 fruits Sensory

1:Extremely soft 2:Very soft 3:Soft
4:Slightly soft 5:Intermediate 6:Slightly
hard 7:Hard 8:Very hard 9:Extremely hard

Hardness of flesh at full or table use maturity

3

Eating quality

5 plants, 5 fruits Sensory

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highEating quality at full or table use maturity

4

Dry matter percentage

5 plants, 5 fruits Obs.&Measr.

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highDry matter percentage of flesh at full or tableof flesh

3:Mealy

4:Intermediate

Flesh texture at full or table use maturity

use maturity. Low:approximately 10-15%,
intermediate:20-25%, high:30-35%

5Keeping quality

5 plants, 5 fruits Observation

1:Extremely low 2:Very low 3:Low 4:Slightly
low 5:Intermediate 6:Slightly high 7:High
8:Very high 9:Extremely highKeeping quality of fruit at table use maturity.
Low: approximately 1 week. Intermediate:1
month, high:3 months

6

Soluble solid content

5 plants, 5 fruits Measurement% (roundto the 1st decimal place)

Brix value of flesh