文部科学省科学研究費補助金「新学術領域研究（研究領域提案型）」「遺伝情報収納・発現・継承の時空間場」
研究成果公表の状況（平成 22 年 8 月現在）
計画研究（平成２０年１１月開始）については平成２１年１月以降の成果、公募研究（平成２１年７月開始）については同年９月以降に公表された成果を記す。

1. 原著論文	  青字は領域内共同研究＜平岡＞	
 
1. Ito, S., Tan L.-J., Andoh, D., Narita, T., Seki, M. Hirano, Y., Narita, K., Kuraoka, I., Hiraoka, Y.
and *Tanaka, K. (2010) MMXD, a TFIIH-independent XPD-MMS19 protein complex involvedin chromosome segregation. Mol. Cell, in press
2. Kawai-Noma, S., Pack, C-G., Kojidani, T., Asakawa, H., Hiraoka, Y., Kinjo, M., Haraguchi, T.,*Taguchi, H., and Hirata, A. (2010) In vivo evidence for the fibrillar structures of Sup35 prions
in yeast cells. J. Cell Biol., 190, 223-231.
3. *Nakano, T., Shuai, J., Koujin, T., Suda, T., Hiraoka, Y., and Haraguchi, T. (2010) Biologicalexcitable media based on non-excitable cells and calcium signaling. Nano Communi. Net., 1,
43-49.
4. Kobayashi, S., Kojidani, T., Osakada, H., Yamamoto, A., Yoshimori, T., Hiraoka, Y., and*Haraguchi, T. (2010) Artificial induction of autophagy around polystyrene beads innonphagocytic cells. Autophagy, 6, 36-45.
5. *Nakano, T., Koujin, T., Suda, T., Hiraoka, Y., Haraguchi, T. (2009) A Locally-Induced
Increase in Intracellular Ca2+ Propagates Cell-to-Cell in the Presence of Plasma Membrane
Ca2+ ATPase Inhibitors in Non-excitable Cells. FEBS Letters, 583, 3593-3599.
6. Murai, T., Nakase, Y., Fukuda, K., Chikashige, Y., Tsutsumi, C., Hiraoka, Y., and *Matsumoto,T. (2009) Distinctive responses to nitrogen starvation in the dominant active mutants of thefission yeast Rheb GTPase. Genetics, 183, 517-527.
7. Chikashige, Y., Yamane, M., Okamasa, K., Tsutsumi, C., Kojidani, T., Sato, M., Haraguchi, T.,
and *Hiraoka, Y. (2009) Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear
envelope to ensure chromosomal bouquet formation. J. Cell Biol., 187, 413-427.
8. *Ogawa, H., Komatsu, T., Hiraoka, Y., and Morohashi, K. (2009) Transcriptional Suppression
by Transient Recruitment of ARIP4 to Sumoylated Nuclear Receptor Ad4BP/SF-1. Mol. Biol.
Cell, 20, 4235-4245.
9. Iwamoto, M., Mori, C., Kojidani, T., Bunai, F., Hori, T., Fukagawa, T., Hiraoka, Y., and*Haraguchi, T. (2009) Two Distinct Repeat Sequences of Nup98 Nucleoporins Characterize
Dual Nuclei in the Binucleated Ciliate Tetrahymena. Curr. Biol., 19, 843-847.
10. *Hiraoka, Y., Kawamata, K., Haraguchi, T., and Chikashige, Y. (2009) Codon usage bias iscorrelated with gene expression levels in the fission yeast Schizosaccharomyces pombe. Genes

1

Cells, 14, 499-509.
11. Hayashi, A., Ding, D-Q., Tsutsumi, C., Chikashige, Y., Masuda, H., Haraguchi, T., and*Hiraoka, Y. (2009) Localization of gene products using a chromosomally-tagged GFP-fusion
library in the fission yeast Schizosaccahromyces pombe. Genes Cells, 14, 217-225.
＜徳永＞	
 1. *Shiina, N., Yamaguchi, K., and *Tokunaga, M. (2010) RNG105 deficiency impairs thedendritic transport of mRNAs for Na+/K+ ATPase subunit isoforms and leads to thedegeneration of neuronal networks. J. Neuroscience, accepted, under revision.
2. *Shiina, N., and *Tokunaga, M. (2010) RNA Granule Protein 140 (RNG140): A Paralog of
RNG105 Localized to Distinct RNA Granules in Neuronal Dendrites in the Adult Vertebrate
Brain. J.Biol. Chem., in press.
3. Miletic, A.V., Graham, D.B., Sakata-Sogawa, K., Hiroshima, M., Hamann, M.J., Cemerski, S.,
Kloeppel, T., Billadeau, D.D., Kanagawa, O., Tokunaga, M., and *Swat, W. (2009) Vav links
the T cell antigen receptor to the actin cytoskeleton and T cell activation independently of
intrinsic Guanine nucleotide exchange activity. PLoS One, 4, e6599, 2009.
4. Fukagawa, A., Hiroshima, M., Sakane, I., and *Tokunaga, M. (2009) Stochastic emergence ofmultiple intermediates detected by single-molecule quasi-static mechanical unfolding ofprotein. Biophysics, 5, 25-35.
＜小布施＞	
 1. Nozawa, R., Nagao, K., Masuda, H, Iwasaki, O., Hirota, T., Nozaki, N., Kimura, H., and*Obuse, C. (2010) Human POGZ modulates HP1 dissociation from mitotic chromosome arms
through Aurora B activation. Nat Cell Biol., 12, 719-27.
2. Takeishi, Y., Ohashi, E., Ogawa, K., Masai, H., Obuse, C. and *Tsurimoto T. (2010) Casein
Kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between humanRad9-Hus1-Rad1 complex and TopBP1. Genes Cells, 15, 761-71.
3. Kiyomitsu, T., Iwasaki, O., Obuse, C. and *Yanagida, M. (2010) Inner Centromere Formation
Requires hMis14, a Trident Kinetochore Protein That Specifically Recruits HP1 to Human
Chromosomes. J. Cell Biol., 188, 791-807.
4. Osakabe, A., Tachiwana, H., Matsunaga, T., Shiga, T., Nozawa, R., Obuse, C., and*Kurumizaka H. (2010) Nucleosome Formation Activity of Human Somatic Nuclear
Autoantigenic Sperm Protein (sNASP). J. Biol. Chem., 285, 11913-11921.
5. Mimura, S., Yamaguchi, T., Ishii, S., Noro, E., Katsura, T., Obuse, C., and *Kamura, T. (2010)
Cul8/Rtt101 Forms a Variety of Protein Complexes that Regulate DNA Damage Response andTranscriptional Silencing. J. Biol. Chem., 285, 9858-9867.
6. Suizu, F., Hiramuki, Y., Okumura, F., Matsuda, M., Okumura, J. A., Hirata, N., Narita, M.,
Kohno, T., Yokota, J., Bohgaki, M., Obuse, C., Hatakeyama, S., Obata, T., and *Noguchi, M.
(2009) The E3 ligase TTC3 Facilitates Ubiquintination and Degradation of Phosphorylated Akt.
Dev. Cell, 17, 800-810.
7. Perpelescu, M., Nozaki, N., Obuse, C., Yang, H., and *Yoda, Y. (2009) Active Establishment
of Centromeric CENP-A Chromatin by RSF Complex. J. Cell Biol., 185, 397-407.
8. Hanyu, Y., Imai, K. K., Kawasaki, Y., Nakamura, T., Nakaseko, Y., Nagao, K., Kokubu, A.,
Ebe, M., Fujisawa, A., Hayashi, T., Obuse, C., and *Yanagida, M. (2009) S. Pombe Cell
Division Cycle Under Limited Glucose Rrequires Ssp1 Kinase, the Putative CaMKK, and2

Sds23, a PP2A-Related Phosphatase Inhibitor. Genes Cells, 14, 539-554.
9. Uehara, R., Nozawa, R., Tomioka, A., Petry, S., Vale, R.D., Obuse, C., and *Goshima, G.
(2009) The Augmin Complex Plays a Critical Role in Spindle Microtubule Generation for
Mitotic Progression and Cytokinesis in Human Cells. Proc. Natl. Acad. Sci. USA, 106,6998-7003.
＜胡桃坂＞	
 1. Shimoyama, S., Nagadoi, A., Tachiwana, H., Yamada, M., Sato, M., Kurumizaka, H., Akashi,S., and Nishimura, Y. (2010) Deimination stabilizes histone H2A/H2B dimers as revealed byelectrospray ionization mass spectrometry. J. Mass Spectrom., in press.
2. Takaku, M., Takahashi, D., Machida, S., Ueno, H., Hosoya, N., Ikawa, S., Miyagawa, K.,
Shibata, T., and *Kurumizaka, H. (2010) Single-stranded DNA catenation mediated by human
EVL and a type I topoisomerase. Nucleic Acids Res., in press.
3. Tachiwana, H., Kagawa, W., Osakabe, A., Kawaguchi, K., Shiga, T., Hayashi-Takanaka, Y.,
Kimura, H., and *Kurumizaka, H. (2010) Structural basis of instability of the nucleosome
containing a testis-specific histone variant, human H3T. Proc. Natl. Acad. Sci. USA, 107,10454-10459.
4. Takizawa, Y., Qing, Y., Takaku, M., Ishida, T., Morozumi, Y., Tsujita, T., Kogame, T., Hirota,
K., Takahashi, M., Shibata, T., *Kurumizaka, H., and *Takeda, S. (2010) GEMIN2 promotes
accumulation of RAD51 at double-strand breaks in homologous recombination. Nucleic Acids
Res., in press.
5. Saito, K., *Kagawa, W., Suzuki, T., Suzuki, H., Yokoyama, S., Saitoh, H., Tashiro, S., Dohmae,N., and *Kurumizaka, H. (2010) The putative nuclear localization signal of the human RAD52
protein is a potential sumoylation site. J. Biochem., 147, 833-842.
6. Osakabe, A., Tachiwana, H., Matsunaga, T., Shiga, T., Nozawa, R., Obuse, C., and*Kurumizaka, H. (2010) Nucleosome formation activity of human sNASP. J. Biol. Chem., 285,
11913-11921.
7. Horikoshi, N., Morozumi, Y., Takaku, M., Takizawa, Y., and *Kurumizaka, H. (2010) Holliday
junction binding activity of human SPF45. Genes Cells, 5, 373-383.
8. Kujjo, L.L., Laine, T., Pereira, R.J.G., Kagawa, W., Kurumizaka, H., Yokoyama, S., and*Perez, G.I. (2010) Enhancing survival of mouse oocytes following chemotherapy or aging bytargeting Bax and Rad51. PLoS one, 12;5(2):e9204.
9. Takaku, M., Machida, S., Nakayama, S., Takahashi, D., and *Kurumizaka, H. (2009)
Biochemical analysis of the human EVL domains in homologous recombination. FEBS J., 276,
5841-5848.
10. Morozumi, Y., Takizawa, Y., Takaku, M., and *Kurumizaka, H. (2009) Human PSF binds to
RAD51 and modulates its homologous-pairing and strand-exchange activities. Nucleic Acids
Res., 37, 4296-4307.
11. Katsura, M., Tsuruga, T., Date, O., Yoshihara, T., Ishida, M., Tomoda, Y., Okajima, M.,
Takaku, M., Kurumizaka, H., Kinomura, A., Mishima, H., and *Miyagawa, K. (2009) The
ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by
Rad51C dysfunction. Nucleic Acids Res., 37, 3959-3968.
12. Takaku, M., Machida, S., Hosoya, N., Nakayama, S., Takizawa, Y., Sakane, I., Shibata, T.,
Miyagawa, K., and *Kurumizaka, H. (2009) Recombination activator function of the novel3

RAD51- and RAD51B-binding protein, Human EVL. J. Biol. Chem., 284, 14326-14336.
13. Ishida, T., Takizawa, Y., Kainuma, T., Inoue, J., Mikawa, T., Shibata, T., Suzuki, H., Tashiro,S., and *Kurumizaka, H. (2009) DIDS, a chemical compound that inhibits RAD51-mediatedhomologous pairing and strand exchange. Nucleic Acids Res., 37, 3367-3376.
14. Shimizu, H., Popova, M., Fleury, F., Kobayashi, M., Hayashi, N., Sakane, I., Kurumizaka, H.,
Venkitaraman, A.R., Takahashi, M., and *Yamamoto, KI. (2009) c-ABL tyrosine kinasestabilizes RAD51 chromatin association. Biochem. Biophys. Res. Commun., 382, 286-291.
15. Hikiba, J., Takizawa, Y., Ikawa, S., Shibata, T., and *Kurumizaka, H. (2009) Biochemical
analysis of the human DMC1-I37N polymorphism. FEBS J., 276, 457-465.
＜末盛＞	
 1. Adachi, K., Suemori, H., Yasuda, S.Y., Nakatsuji, N., and *Kawase, E. (2010) Role of SOX2 inmaintaining pluripotency of human embryonic stem cells. Genes Cells, 15, 455-470.
2. International Stem Cell Initiative Consortium, Akopian, V., *Andrews, P.W., Beil, S.,
Benvenisty, N., Brehm, J., Christie, M., Ford, A., Fox, V., Gokhale, P.J., Healy, L., Holm, F.,
Hovatta, O., Knowles, B.B., Ludwig, T.E., McKay, R.D., Miyazaki, T., Nakatsuji, N., Oh, S.K.,
Pera, M.F., Rossant, J., Stacey, G.N., and Suemori, H. (2010) Comparison of defined culture
systems for feeder cell free propagation of human embryonic stem cells. In Vitro Cell Dev
Biol Anim., 46, 247-258.
3. Ishii, T., Yasuchika, K., Fukumitsu, K., Kawamoto, T., Kawamura-Saitoh, M., Amagai, Y., Ikai,
I., Uemoto, S., Kawase, E., Suemori, H., and *Nakatsuji, N. (2010) In vitro hepatic maturationof human embryonic stem cells by using a mesenchymal cell line derived from murine fetallivers. Cell Tissue Res., 339, 505-512.
4. Mitsui, K., Suzuki, K., Aizawa, E., Kawase, E., Suemori, H., Nakatsuji, N., and *Mitani, K.
(2009) Gene targeting in human pluripotent stem cells with adeno-associated virus vectors.
Biochem. Biophys. Res. Commun., 388, 711-717.
5. Taura, D., Noguchi, M., Sone, M., Hosoda, K., Mori, E., Okada, Y., Takahashi, K., Homma, K.,
Oyamada, N., Inuzuka, M., Sonoyama, T., Ebihara, K., Tamura, N., Itoh, H., Suemori, H.,
Nakatsuji, N., Okano, H., Yamanaka, S., and *Nakao, K. (2009) Adipogenic differentiation ofhuman induced pluripotent stem cells: comparison with that of human embryonic stem cells.
FEBS Lett., 583, 1029-33.
6. Yamauchi, K., Hasegawa, K., Chuma, S., Nakatsuji, N., and *Suemori, H. (2009) In vitro germ
cell differentiation from cynomolgus monkey embryonic stem cells. PLoS ONE, 4, e5338.
7. Fukumitsu, K., Ishii, T., Yasuchika, K., Amagai, Y., Saitoh, M., Kawamoto, T., Kawase, E.,
Suemori, H., Nakatsuji, N., Ikai, I., and *Uemoto, S. (2009) Tissue Eng Part A, 15, 3847-56.
＜大山＞	
 
1.
Tanase, J., Morohashi, N., Fujita, M., Nishikawa, J., *Shimizu, M., and *Ohyama, T. (2010)
Highly efficient chromatin transcription induced by superhelically curved DNA segments: theunderlying mechanism revealed by a yeast system. Biochemistry, 49, 2351–2358.
2.
Tanase, J., Mitani, T., Udagawa, K., Nishikawa, J. and *Ohyama, T. (2010) Competence of an
artificial bent DNA as a transcriptional activator in mouse ES cells. Mol. Biol. Rep., in press.
3.
*Kamiya, H., Fukunaga, S., Ohyama, T., and Harashima, H. (2009) Effects of carriers ontransgene expression from plasmids containing a DNA sequence with high histone affinity. Int.
J. Pharm., 376, 99-103.

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4.

Kitayama, K., Kamo, M., Oma, Y., Matsuda, R., Uchida, T., Ikura, T., Tashiro, S., Ohyama,T., Winsor, B., and *Harata, M. (2009) The human actin-related protein hArp5:
nucleo-cytoplasmic shuttling and involvement in DNA repair. Exp. Cell Res., 315, 206-217.
＜木村(暁)＞	
 1. Oginuma, M., Takahashi, Y., Kitajima, S., Kiso, M., Kanno, J., Kimura, A., and *Saga Y.
(2010) The oscillation of Notch activation but not its boundary is required for somite borderformation and rostral-caudal patterning within a somite. Development, 137, 1515-1522.
2. Hara, Y., and *Kimura, A. (2009) Cell-size-dependent spindle elongation in the Caenorhabditis
elegans early embryo. Curr. Biol., 19, 1549-1554.
＜片山＞	
 1. *Kang, J-H., Oishi, J., Kim, J-H., Ijuin, M., Toita, R., Jun, B., Asai, D., Mori, T., Niidome, T.,
Tanizawa, K., Kuroda, S., and *Katayama, Y. (2010) Hepatoma-targeted gene delivery using atumor cell-specific gene regulation system combined with a human liver cell-specificbionanocapsule. Nanomedicine: NBM., in press.
2. *Sato, Y., Kawamura, K., Niidome, T., and *Katayama, Y. (2010) Characterization of gene
expression regulation using D-RECS polymer by enzymatic reaction for an effective design of
enzyme-responsive gene regulator. J. Control. Release, 143, 344-349.
3. *Asai, D., Kuramoto, M., Shoji, Y., Kodama, K., Kang, J-H., Kawamura, K., Miyoshi, H.,
Mori, T., Niidome, T., Nakashima, H., and *Katayama, Y. (2009) Specific transgene expression
in HIV-infected cells using protease-cleavable transcription regulator. J. Control. Release, 141,52-61.
4. Kang, J-H., Asai, D., Toita, R., Kitazaki, H., and *Katayama, Y, (2009) Plasma protein kinase
C (PKC) a as a biomarler for the diagnosis of cancers. Carcinogenesis, 30, 1927-1931.
5. Toita, R., Kang, J-H., Kim, J-H., Tomiyama, T., Mori, T., Niidome, T., Jun, B., and *Katayama,
Y. (2009) Protein kinase Cα-specific peptide substrate graft-type copolymer for cancercell-specific gene regulation systems. J. Control. Release, 139, 133-139.
6. Kang, J-H., Toita, R., Tomiyama, T., Oishi, J., Asai, D., Mori, T., Niidome, T., and *Katayama,
Y. (2009) Cellular signal-specific peptide substrate is essential for the gene delivery system
responding to cellular signals. Bioorg. Med. Chem. Lett., 19, 6082-6086.
7. Asai, D., Tsuchiya, A., Kang, J-H., Kawamura, K., Oishi, J., Mori, T., Niidome, T., Shoji,Y.,
Nakashima, H., and *Katayama, Y. (2009) Inflammatory cell-specific gene regulation system
responding to Ikappa-B kinase beta activation. J. Gene Med., 11, 624-632.
8. Kawamura, K., Kuramoto, M., Mori, T., Toita, R., Oishi, J., Sato, Y., Kang, J-H., Asai, D.,
Niidome, T., and *Katayama, Y. (2009) Molecularmechanism of Caspase-3-induced gene
expression of polyplexes formed polycations grafted with cationic substrate peptides. J.
Biomater. Sci. Polym. Ed., 20, 967-980.
9. Asai, D., Kang, J-H., Toita, R., Tsuchiya, A., Niidome, T., Nakashima, H., and *Katayama, Y.
(2009) Regulation of transgene expression in tumor cells by exploiting endogenous intracellularsignals. Nanoscale Res. Lett., 4, 229-233.
10. Kang, J-H., Mori, T., Niidome, T., and *Katayama, Y. A syngeneic hepatocellular carcinoma
model rapidly and simply prepared using a hydrodynamics-based procedure. Vet. J., 181,336-339.
＜木村(宏)＞	 5

1.

Kelly, A.E., Ghenoiu, C., Xue, J.Z., Zierhut, C., Kimura, H., and *Funabiki, H. (2010) Survivin
reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B. Science,in press
2. Nozawa, R.S., Nagao, K., Masuda, H.T., Iwasaki, O., Hirota, T., Nozaki, N., Kimura, H., and*Obuse, C. (2010) Human POGZ modulates HP1 dissociation from mitotic chromosome arms
through Aurora B activation. Nature Cell. Biol., 12, 719-727.
3. Tachiwana, H., Kagawa, W., Osakabe, A., Kawaguchi, K., Shiga, T., Hayashi-Takanaka, Y.,
Kimura, H., and *Kurumizaka, H. (2010) Structural basis of instability of the nucleosome
containing a testis-specific histone variant, human H3T. Proc. Natl. Acad. Sci. USA, 107,10454-10459.
4. Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M.,
Lorincz, M.C., and *Shinkai, Y. (2010) Proviral silencing in embryonic stem cells requires thehistone methyltransferase ESET. Nature, 464, 927-931.
5. Hashimoto, H., Takami, Y., Sonoda, E., Iwasaki, T., Iwano, H., Tachibana, M., Takeda, S.,
Nakayama, T., *Kimura, H., and *Shinkai, Y. (2010) Histone H1 null vertebrate cells exhibit
altered nucleosome architecture. Nucleic Acids Res., 38, 3533-3545.
6. Numa, H., Kim, J.M., Matsui, A., Kurihara, Y., Morosawa, T., Ishida, J., Mochizuki, Y.,
Kimura, H., Shinozaki, K., Toyoda, T., Seki, M., Yoshikawa, M., and *Habu, Y. (2010)
Transduction of RNA-directed DNA methylation signals to repressive histone marks in
Arabidopsis thaliana. EMBO J., 29, 352-362.
7. Hayashi-Takanaka, Y., Yamagata, K., Nozaki, N., and *Kimura, H. (2009) Visualizing histone
modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase.
J. Cell Biol., 187, 781-790.
8. Goto, Y. and *Kimura, H. (2009) Inactive X chromosome-specific histone H3 modifications
and CpG hypomethylation flank a chromatin boundary between an X-inactivated and an escapegene. Nucleic Acids Res., 37, 7416-7428.
9. Wada, Y., Ohta, Y., Xu, M., Tsutsumi, S., Minami, T., Inoue, K., Komura, D., Kitakami, J.,
Oshida, N, Papantonis, A., Izumi, A., Kobayashi, M., Meguro, H., Kanki, Y., Mimura, I.,
Yamamoto, K., Mataki, C., Hamakubo, T., Shirahige, K., Aburatani, H., Kimura, H., *Kodama,T., *Cook, P.R., and Ihara, S. (2009) A wave of nascent transcription on activated humangenes. Proc. Natl Acad. Sci. USA, 106, 18357-18361.
＜小沢田＞	
 1. Konno, K., *Kosawada, T., Suzuki, M., Nakamura, T., Feng, Z., Hozumi, Y., and Goto, K.
(2010) Dynamic actuation and sensing micro-device for mechanical response of cultured
adhesive cells. Microsystem Technologies 16-6, 993-1000.
＜斉藤＞	
 1. Tei, S., Saitoh, N., Funahara, T., Iida, I., Nakatsu, Y., Kinoshita K., Kinoshita, Y., Saya, H., and*Nakao, M. (2009) Simian virus 40 large T antigen targets the microtubule- stabilizing protein
TACC2. J. Cell Sci., 122, 3190-3198.
2. Liu, L., Ishihara, K., Ichimura, T., Fujita, N., Hino, S., Tomita, S., Watanabe, S., Saitoh, N., Ito,T., and *Nakao, M. (2009) MCAF1/AM is involved in Sp1-mediated maintenance ofcancer-associated telomerase activity. J. Biol. Chem., 284, 5165-5174.
＜関＞	
 

6

1.

Endo, H., Kawashima, S., Sato, L., Lai, M.S., Enomoto, T., *Seki, M., and *Horikoshi, M.
Chromatin dynamics mediated by histone modifiers and histone chaperones in post-replicative
recombination. Genes Cells, in press.
2. Inoue, E., Tano, K., Yoshii, H., Nakamura, J., Tada, S., Watanabe, M., *Seki, M., and*Enomoto, T. SOD1 is essential for the viability of DT40 cells and nuclear SOD1 functions as
a guardian of genomic DNA. J. Nucleic Acid, in press.
3. Kundu, L.R., Kumata, Y., Kakusho, N., Watanabe, S., Furukohri, A., Waga, S., Seki, M., Masai, H.,
Enomoto, T., and *Tada, S. Deregulated Cdc6 inhibits DNA replication and suppresses
Cdc7-mediated phosphorylation of Mcm2-7 complex. Nucleic Acids Res., in press.
4. Sakamoto, M., Noguchi, S., Kawashima, S., Okada, Y., Enomoto, T., Seki, M., and *Horikoshi,
M. (2009) Global analysis of mutual interaction surfaces of nucleosomes with comprehensive
point mutants. Genes Cells,14, 1271-1330.
5. Yoshimura, A., *Seki, M., Kanamori, M., Tateishi, S., Tsurimoto, T., Tada, S., and Enomoto, T.
(2009) Physical and functional interaction between WRNIP1 and RAD18. Genes Genet.
System, 84, 171-178.
6. Ohuchi, T., *Seki, M., Kugou, K., Tada, S., Ohta, K., and Enomoto, T. (2009) Accumulation ofsumoylated Rad52 in checkpoint mutants perturbed in DNA replication. DNA Repair, 8,690-696.
7. Takada, S., Inoue, E., Tano, K., Yoshii, H., Abe, T., Yoshimura, A., Akita, M., Tada, S.,
Watanabe, M., *Seki, M., and Enomoto, T. (2009) Generation and characterization of cells thatcan be conditionally depleted of mitochondrial SOD2. Biochem. Biophys. Res. Commun., 379,
233-238.
8. Tsuyama, T., Watanabe, S., Aoki, A., Cho, Y., Seki, M., Enomoto, T., and *Tada, S. (2009)
Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in
Xenopus egg extracts. Mol. Biol. Cell, 20, 937-947.
＜田代＞	
 1. Saito, K., Kagawa, W., Suzuki, T., Suzuki, H., Yokoyama, S., Saitoh, H., Tashiro, S., Dohmae,N., and *Kurumizaka, H. (2010) The putative nuclear localization signal of the human RAD52
protein is a potential sumoylation site. J. Biochem., 147, 833-842.
2. Niida, H., Katsuno, Y., Sengoku, M., Shimada, M., Yukawa, M., Ikura, M., Ikura, T., Kohno,
K., Shima, H., Suzuki, H., Tashiro, S., and *Nakanishi, M. (2010) Essential role ofTip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair
during G1 phase. Genes Dev., 24, 333-338.
3. Ishida, T., Takizawa, Y., Kainuma, T., Inoue, J., Mikawa, T., Shibata, T., Suzuki, H., Tashiro,S., and *Kurumizaka, H. (2009) DIDS, a chemical compound that inhibits RAD51-mediatedhomologous pairing and strand exchange. Nucleic Acids Res., 37, 3367-3376.
＜長山＞	
 1. *Nagayama K., and Matsumoto, T. (2010) Estimation of Single Stress Fiber Stiffness in
Cultured Aortic Smooth Muscle Cells under Relaxed and Contracted States: Its Relation to
Dynamic Rearrangement of Stress Fibers. J Biomech., 43, 1443-1449.
2. *Matsumoto, T., Ikuta, N., Mori, M., and Nagayama, K. (2010) Mechanics of wrinkle
formation: Micromechanical analysis of skin deformation during wrinkle formation in
UV-irradiated mice. Skin Res. Technol., 16, 179-189.

7

3.

*Nagayama, K., Morishima, N., and Matsumoto, T. (2009) Effects of three-dimensional culture
and cyclic stretch stimulation on expression of contractile proteins in freshly isolated rat aortic
smooth muscle cells. J. Biomech. Sci. Eng., 4, 286-297.
＜原田＞	
 1. Philimonenko, V.V., Janacek, J., Harata, M., and *Hozak P. (2010) Transcription-dependent
rearrangements of actin and nuclear myosin I in the nucleolus. Histochem. Cell Biol., in press
2. Matsuda, R., Hori, T., Kitamura, H., Takeuchi, K., Fukagawa, T., and *Harata, M.
Identification and characterization of two isoforms of the vertebrate H2A.Z histone variant.
Nucleic Acids Res., in press.
3. Yoshida, Y., Shimada, K., Oma, Y., Kalck, V., Akimura, K., Taddei, A., Iwahashi, H., Kugou,
K., Ohta, K., Gasser, S.M., and *Harata, M. (2010) Actin-related protein Arp6 influences
H2AZ-dependent and -independent gene expression and links ribosomal protein genes tonuclear pores. PLoS Genet., 6, e1000910.
4. Kumeta, M., Yoshimura, S.H., Harata, M., and *Takeyasu, K. (2010) Molecular mechanisms
underlying nucleocytoplasmic shuttling of actinin-4. J. Cell Sci. 123, 1020-1030.
＜廣田＞	
 1. Kohzaki, M., Nishihara, K., Hirota, K., Sonoda, E., Yoshimura, M., Ekino, S., Butler, J.E.,
Watanabe, M., Halazonetis, T.D., and *Takeda, S. DNA polymerases nu and theta are required
for efficient immunoglobulin V gene diversification in chicken. J. Cell Biol., 189, 1117-1127.
2. Takizawa, Y., Qing, Y., Takaku, M., Ishida, T., Morozumi, Y., Tsujita, T., Kogame, T., Hirota,
K., Takahashi, M., Shibata, T., *Kurumizaka, H., and *Takeda, S. GEMIN2 promotes
accumulation of RAD51 at double-strand breaks in homologous recombination. Nucleic Acids
Res., in press.
3. Kikuchi, K., Abdel-Aziz, H.I., Taniguchi, Y., Yamazoe, M., Takeda, S., and *Hirota, K. (2009)
Bloom DNA helicase facilitates homologous recombination between diverged homologous
sequences. J. Biol Chem., 284, 26360-2636.
＜前島＞	
 
1.
*Maeshima, K., Iino, H., Hihara, S., Funakoshi, T., Watanabe, A., Nishimura, Y., Nakatomi,R., Yahata, K., Imamoto, F., Hashikawa, T., Yokota, H., and *Imamoto, N. (2010) Nuclear
pore formation but not nuclear growth is governed by cyclin-dependent Kinases (Cdks) during
interphase. Nat. Struct. Mol. Biol., in press.
2.
Matsuyama, S., Shimura, M., Fujii, M., Maeshima, K., Yumoto, H., Mimura, H., Sano, Y.,
Yabashi, M., Nishino, Y., Tamasaku, K., Ishizaka, Y., Ishikawa, T., and *Yamauchi, K. (2010)
Elemental mapping of frozen hydrated cells with cryo-scanning X-ray fluorescencemicroscopy. X-Ray Spectrometry, in press.
3.
Iino, H., *Maeshima, K., Nakatomi, R., Kose, S., Hashikawa, T., Tachibana, T., and *Imamoto,N. (2010) Live imaging system for visualizing nuclear pore complex (NPC) formation during
interphase in mammalian cells. Genes Cells, 15, 647-660.
4.
Takemoto, A., Maeshima, K., Ikehara, T., Yamaguchi, K., Murayama, A., Imamura, S.,
Imamoto, N., Yokoyama, S., Hirano, T., Watanabe, Y., Hanaoka, F., Yanagisawa, J., and*Kimura, K. (2009) The chromosomal association of condensin II is regulated by anon-catalytic action of PP2A. Nat. Struct. Mol. Biol., 16, 1302-1308.
＜松浦＞	
 

8

1.

Matsui, A., and *Matsuura, A. (2010) Cell size regulation during telomere-directed senescencein Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem., 74, 195-198.
2. Weng, Y., Xiang, L., Matsuura, A., Zhang, Y., Huang, Q., and *Qi, J. (2010) Ganodermasides
A and B, two novel anti-aging ergosterols from spores of a medicinal mushroom Ganoderma
lucidum on yeast via UTH1 gene. Bioorg. Med. Chem., 18, 999-1002.
＜三國＞	
 1. Sun, F., Mikuni, S., and *Kinjo, M. (2010) Simultaneous measurement of caspase-3 andcaspase-9 activities during induced apoptosis by fluorescence cross-correlation spectroscopy.
Bioimages, in press.
2. Ohtake, N., *Niikura, K., Suzuki, T., Mikuni, S., Matsuo, Y., Nagakawa, K., Kinjo, M., Sawa,
H., and *Ijiro, K. (2010) Low pH-triggered Drug-model Molecule Release from Virus-Like
Particles. ChemBioChem, 11, 959-962.
＜守屋＞	
 
1.
Kaizu, K., *Moriya, H., and Kitano, H. (2010) Fragilities caused by dosage imbalance inregulation of the budding yeast cell cycle. PLoS Genet., 6, e1000919.
2.
Chino, A., Watanabe, K., and *Moriya, H. (2010) Plasmid Construction Using Recombination
Activity in the Fission Yeast Schizosaccharomyces pombe. PLoS One, 5, e9652.
＜山縣＞	
 1. *Yamagata, K., Ueda, J., Mizutani, E., Saitou, M., and Wakayama, T. (2010) Survival anddeath of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell
imaging with an Oct4 reporter system. Dev. Biol., in press.
2. Okada, Y., Yamagata, K., Hong, K., Wakayama, T., and *Zhang, Y. (2010) A role for theelongator complex in zygotic paternal genome demethylation. Nature, 463, 554-558.
3. Hayashi-Takanaka, Y., Yamagata, K., Nozaki, N., and *Kimura, H. (2009) Visualizing histone
modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase.
J. Cell Biol., 187, 781-790.
4. Wakayama, S., Kawahara, Y., Li, C., Yamagata, K., Yuge, L., and *Wakayama, T. (2009)
Detrimental effects of microgravity on mouse preimplantation development in vitro. PLoS One,4, e6753.

9

2. 総説など	  青字は領域内共同研究＜平岡＞	
 1. Chikashige, Y., Haraguchi, T. and *Hiraoka, Y. (2010) Nuclear envelope attachment is notnecessary for telomere function in fission yeast. Nucleus, in press.
2. Iwamoto, M., Asakawa, H., Hiraoka, Y., and *Haraguchi, T. (2010) The nucleoporin Nup98: agatekeeper in the eukaryotic kingdoms. Genes Cells, 15, 661-669.
3. Ding, D-Q., Haraguchi, T., and *Hiraoka, Y. (2010) Alignment and recognition of homologous
chromosomes in meiosis. FEBS J., 277, 565-570.
4. *Hiraoka, Y., and Dernburg, A. (2009) The SUN rises on meiotic chromosome dynamics. Dev.
Cell, 17, 598-605.
5. Asakawa, H., and *Hiraoka, Y. (2009) Live-cell fluorescence imaging of meiotic chromosome
dynamics in Schizosaccharomyces pombe. Meth. Mol. Biol., 558, 53-64.
＜小布施＞	
 1.
野澤竜介，長尾恒治，小布施力史（2010）ヒト HP1 結合タンパク質 POGZ は分裂前期の Aurora B キナーゼの染色体腕部での活性化に寄与する．細 胞 工 学 ，29，印刷中
2.
長尾恒治，野澤竜介，小布施力史（2009）染色体を構築する複合体のプロテオミクス
解析．細 胞 工 学 （ 別 冊 ； 明 日 を 拓 く 新 次 元 プ ロ テ オ ミ ク ス ） ，81-90.
3.
野澤竜介，小布施力史（2009）ヘテロクロマチン結合因子とその機能．実 験 医 学（ 増刊 ； 細 胞 核 −遺 伝 情 報 制 御 と 疾 患 ），27, 123-130.
4.
清光智美，小布施力史，柳田充弘（2009）ブリンキン-紡錘体形成チェックポイントと微小管結合に必須なキネトコア蛋白質、蛋 白 質・核 酸・酵 素（ 増 刊；染 色 体 サ イ クル ） 54, 421-426.
＜胡桃坂＞	
 1. *Kagawa, W., and *Kurumizaka, H. (2010) Dynamics in the transmission of genetic
information: from meiosis to postmeiotic events. FEBS J., 277, 564.
2. *Kagawa, W., and *Kurumizaka, H. (2010) From meiosis to postmeiotic events: Uncovering
the molecular roles of the meiosis-specific recombinase Dmc1. FEBS J., 277, 590-598.
＜末盛＞	
 1. 高田圭、末盛博文 (2010) 再生医療の最前線 2010「再生医療に適した ES 細胞培養シス
テム」実 験 医 学 28, 204-208
＜大山＞	
 
1.
*大山隆, 木村元, 下岡保俊 (2009)	
  ゲノム DNA はいかにして折り畳まれるか—DNA
物 性 と ゲ ノ ム 収 納 —. 実 験 医 学 増 刊 号 「 細 胞 核 — 遺 伝 情 報 制 御 と 疾 患 」 , 27,2715-2722.
＜木村(暁)＞	
 1. Kimura, A., and *Onami, S. (2010) Modeling microtubule-mediated forces and centrosome
positioning in Caenorhabditis elegans embryos. Methods in Cell Biol., 97, in press.
2. *Goshima, G., and *Kimura, A. (2009) New look inside the spindle: microtubule-dependent
microtubule generation within the spindle. Curr. Opin. Cell. Biol., 22, 44-49.
＜小穴＞	
 
1.
*小穴英廣、寺尾京平、森泰啓、佐藤吉信、鷲津正夫 (2010) DNA 単分子解析を目指した DNA 分子マイクロマニピュレーション技術の開発、電 気 学 会 研 究 会 資 料 、 バ イ
オ ・ マ イ ク ロ シ ス テ ム 研 究 会 	
  BMS-10-002, 5-9.

10

＜片山＞	
 1.
Kang, J-H., Toita R., and *Katayama, Y. (2010) Bio and nanotechnological strategies fortumor-targeted gene therapy. Biotechnilogy Advances, in press.
＜木村(宏)＞	
 1. *Kimura, H., Hayashi-Takanaka, Y., and Yamagata, K. (2010) Visualization of DNA
methylation and histone modifications in living cells. Curr. Opin. Cell Biol., 22, 412-418.
2. 木村宏、林陽子 (2010) 特異的モノクローナル抗体を用いたメチル化ヒストンダイナミ
クスの可視化 実 験 医 学 （ 増 刊 号 ） , 印刷中
3. 木村宏、林陽子 (2009) ヒストンダイナミクスの可視化と膜透過化細胞による再構成.
実 験 医 学 （ 増 刊 号 ） 27, 2833-2838.
＜河野＞	
 1. *米谷佳晃、河野秀俊 DNA の水和とダイナミクスの塩基配列依存性：DNA -タンパク質、DNA‐低分子認識の理解に向けて Medical Bio 別 冊 「 揺 ら ぎ と 生 体 機 能 － 体 温 環 境
下 に お け る 生 命 分 子 の 機 能 発 現 」 , 印刷中＜斉藤＞	
 
1. 徳永和明、斉藤典子、中尾光善（2009）組織幹細胞におけるエピジェネティックな制
御機構、最 新 医 学 , 64, 52-63.
2. 斉藤典子、徳永和明、冨田さおり、中尾光善（2009）核異型の背景にあるエピジェネ
ティクスのダイナミズム、病 理 と 臨 床 , 27, 653-658.
＜滝沢＞	
 1. *滝沢琢己, 高木美智, 笹岡寛敏 (2010) ゲノム DNA の核内配置と遺伝子発現制御.	
  生
化 学 , 82, 143-149.
＜田代＞	
 1. 田代聡、井倉毅 (2009) ゲノムストレス応答と核内タンパク質の高次構造体構築. 実 験
医 学 増 刊 , 27, 173-178.
＜原田＞	
 1. 尾間由佳子、松田涼、北村大志、*原田昌彦 (2009) クロマチン・細胞核の機能ドメイ
ン形成. 実 験 医 学 , 27, 2752-2758.
＜廣田＞	
 1. 廣田耕志、武田俊一 (2010) 基礎の基礎	
  細 胞 工 学 (１月号).
＜前島＞	
 
1.
*Maeshima, K., Hihara, S., and Eltsov, M. (2010) Chromatin structure: does the 30-nm fibre
exist in vivo? Current Opinion in Cell Biology, 22, 291-297.
2.
日原さえら、前島一博 (2009)、染色体の構築原理、実 験 医 学 （ 増 刊 ） , 27, 43-51.
3.
前島一博、西野吉則	
  (2009)	
  コヒーレント X 線回折によるヒト染色体構造の 3 次元観察. 生 物 物 理 (10 月号), 49, 298-300.
＜松浦＞	
 1. 松浦彰 (2009)「テロメア機能構造の時空間的制御機構」	
  実 験 医 学 増 刊「 細 胞 核 −遺伝 情 報 の 制 御 機 構 と 疾 患 」 27, 66-71.
2. 松浦彰 (2009)「酵母を用いた老化研究」 大澤俊彦、丸山和佳子監修「 脳 内 老 化 制 御と バ イ オ マ ー カ ー -基 盤 研 究 と 食 品 素 材 」 (シーエムシー出版) pp.23-32.
＜三國＞	
 1. Kinjo, M., Sakata, H., and Mikuni, S. (2010) First steps for fluorescence correlation11

spectroscopy of living cells. Live Cell Imaging: A Laboratory Manual, Second Edition,229-238.
＜安原＞	
 1. Yasuhara, N., and *Yoneda, Y. (2010) Regulation of Signaling and Cellular Fate throughModulation of Nuclear Protein Import. BBA - Molecular Cell Research, in press.
＜山縣＞	
 1. *Yamagata, K. (2010) DNA methylation profiling using live-cell imaging. Methods, in press.
2. *Kimura, H., Hayashi-Takanaka, Y., and Yamagata, K. (2010) Visualization of DNA
methylation and histone modifications in living cells. Curr. Opin. Cell Biol., 22, 412-418.
3. *山縣一夫、若山照彦	
  ビオフィリア第１９号	
  【特集】 生き物を形作るしくみ―エ
ピジェネティクス―・クローン研究でわかってきた生殖細胞の謎―エピジェネティッ
クメモリー―
4. *山縣一夫	  実 験 医 学 増 刊 号 「 細 胞 核 ― 遺 伝 情 報 制 御 と 疾 患 」 	
  ４章－１「受精・初期胚細胞核ダイナミクスと発生・出生不全」

12

3. 特許出願＜徳永＞	
 
発明の名称： 「顕微鏡システム」
出願番号:
特願 2010-145571
発明者:	
  	
  	
  宮脇成礼、徳永万喜洋、十川久美子、江部康平、堀博文
出願人:	
  	
  	
  オリンパスソフトウェアテクノロジー株式会社、国立大学法人東京工業大学、
独立行政法人理化学研究所
出願日:	
  	
  	
  平成２２年６月２５日＜斉藤＞	
 発明の名称： 「細胞核を構成する構造体の解析方法、及び細胞核の形態の解析方法」
出願番号:
特願 2009-223587
発明者:	
  	
  	
  村瀬八重子、小林民代、天川玄太、中尾光善、斉藤典子、徳永和明
出願人:	
  	
  	
  オリンパス株式会社、国立大学法人熊本大学
出願日:	
  	
  	
  平成２１年９月２９日

13