ูグᵝᘧ➨  ྕ㸦➨  ᮲➨  㡯㸪➨  ᮲➨  㡯㛵ಀ㸧ㄽ ᩥ ᑂ ᰝ ࡢ ⤖ ᯝ ࡢ せ ᪨༤ኈࡢᑓᨷศ㔝ࡢྡ⛠ ༤ኈ㸦 ཱྀ⭍೺ᗣ⛉Ꮫ 㸧Ꮫ఩つ๎➨  ᮲➨ ࣭ 㡯ヱᙜ Ặྡ  ⸽ ᢎ⩢Ꮫ ఩ ᤵ ୚ ࡢ ᮲ ௳ㄽ ᩥ 㢟 ┠
Association of oral Epstein-Barr virus with oral health status in Japanese adults㸦᪥ᮏேᡂே࡟࠾ࡅࡿཱྀ⭍ࡢ Epstein-Barr ࢘࢖ࣝࢫ࡜ཱྀ⭍ࡢ೺ᗣ≧ែ࡜ࡢ㛵ಀ㸧ㄽᩥᑂᰝᢸᙜ⪅୺  ᰝ   ᩍᤵ ኴ⏣ ⪔ྖ               ༳ᑂᰝጤဨ   ᩍᤵ ஧ᕝ ᾈᶞ                   ᑂᰝጤဨ   ᩍᤵ Ỉ㔝 ᬛோ                   ࠝㄽᩥᑂᰝࡢ⤖ᯝࡢせ᪨ࠞ

Epstein-Barr virus (EBV) is a member of the herpesviridae family. It remains unclear
whether EBV is related to poor oral health among middle-aged and older Japanese people.
The aim of this study was to clarify the relationship between oral EBV and oral healthstatus in Japanese adults.
A total of 124 patients who visited the Department of Oral Health of Hiroshima
University Hospital were analyzed in this study. In addition, 43 dependent older people
who had a certified need for long-term support or nursing care were enrolled. Oral rinse
samples were obtained by asking the subjects to rinse their mouths with saline. Orcellex®
Brush (Rovers Medical Devices) was employed to collect samples from the tongue surface
in dependent older people. Real time PCR analysis was performed to determine EBV DNA
copy number. PCR amplification of the bacterial 16S rRNA gene was performed to detect
periodontal disease-related bacteria. Propensity scores were calculated by logistic
regression analysis of 11 clinical factors (age, sex, remaining teeth, denture use, smoking,hypertension, diabetes, hyperlipidemia, stroke, heart disease, and bone and joint disease).
Statistical analysis was performed using SPSS version 24.0. P < 0.05 was considered toindicate a statistically significant difference.
EBV DNA was detected in 16 of 124 participants (12.9%). Ten of the 38 participants
with periodontal pockets ≥ 6 mm were EBV DNA positive (26.3%). There was a significant
association between EBV DNA positivity and probing depth (P = 0.01). In addition, there
was a significant association between bleeding on probing (BOP) and EBV DNA positivity(P = 0.03). EBV DNA copy number was investigated in 16 EBV positive cases. A
significant association was not found between EBV DNA copy number and periodontal
pocket depth or BOP. Next, propensity score matching was performed between participants

with ≥ 4 mm periodontal pockets, BOP or both (i.e., participants with poor periodontalhealth) and those without ≥ 4 mm periodontal pockets and BOP (i.e., participants with goodperiodontal health) to investigate the relationship between EBV and periodontal healthstatus. Thirty-five matched pairs were identified among the participants. Participants withpoor periodontal health exhibited a higher EBV DNA positivity rate (25.7%) than thosewith good periodontal health (0.0%). A significant association was found between EBVDNA positivity and periodontal health status (P = 0.001). Total 65 samples were collectedby inserting sterilized paper points into the periodontal pocket. Sterilized paper points wereemployed to obtain gingival crevicular fluid. Of 65 cases, 6 cases showed EBV DNA
positive. Patients with ≥ 4 mm periodontal pockets with BOP recorded a higher EBV DNA
positivity rate (15.8%) than those without ≥ 4 mm periodontal pockets with BOP (6.5%).
However, no significant difference was found between EBV DNA positivity and BOP orperiodontal pockets with BOP. These results suggest the presence of EBV in periodontal
pocket.
EBV DNA was detected in 3 of 43 dependent older people (7.0%). EBV DNA was not
significantly associated with clinical factors. People with poor oral hygiene showed
increased EBV DNA-positive rates (33.3%) compared with those with good or fair oralhygiene statuses (9.1% and 3.4%, respectively), but the association was not significant.
The results of this study suggest that EBV localizes in periodontal pockets. The oralcavity is thought to be an initial site for EBV infection. An inhibited local immune system
and local periodontal inflammation may provide the chance for EBV to infect oral epithelialcells.
௨ୖࡢ⤖ᯝ࠿ࡽ㸪ᮏㄽᩥࡣ୰㧗ᖺࡢ᪥ᮏே࡟࠾ࡅࡿ Epstein-Barr ࢘࢖ࣝࢫ࡜ṑ࿘⑓ࡢ㛵ಀ࡟ࡘ࠸࡚᪂つࡢ▱ぢ࡟ࡘ࠸࡚㏙࡭ࡓࡶࡢ࡜ㄆࡵࡽࢀࡓࠋࡼࡗ࡚ᑂᰝጤဨ఍ጤဨ඲ဨࡣ㸪ᮏㄽᩥࡀ⸽ᢎ⩢࡟༤ኈ㸦ཱྀ⭍೺ᗣ⛉Ꮫ㸧ࡢᏛ఩ࢆᤵ୚ࡍࡿ࡟༑ศ࡞౯್ࡢ࠶ࡿࡶࡢ࡜ㄆࡵࡓࠋ