04pB03
プラズマ表面機能化酸化亜鉛ナノ材料に固定化した糖鎖の光学的検出
Optical detection of sugar chains connected to plasma aminated zinc oxidenanomaterials
チョラン ミハイ アレクサンドル1,2、モトレスク イウリアナ3、ルカ ドミトッル2、永津 雅章1Mihai Alexandru Ciolan1,2, Iuliana Motrescu2, Dumitru Luca2, Masaaki Nagatsu1
1Graduate School of Science and Technology, Shizuoka University,2

3

Department of Physics, “Alexandru Ioan Cuza” University, 11, Carol I blvd., 700506-Iasi, Romania
Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Nakaku, Hamamatsu 432-8011, Japan1

静大創造科技院, 2アレクサンドルイワンクザ大学、ルーマニア, ３静大電子工研

In our previous studies we have proposed
the use of zinc oxide (ZnO) nanoparticles forbioimaging
applications:
nanoparticles
areproduced by pulsed laser ablation, amine groups areintroduced on their surface using dry plasma
processing, and then biomolecules are connected tothe amine functionalities. The preparation steps areschematically presented in Fig. 1.
NH3 plasma(H, N, NH, NH2,etc.)

Targetmolecule

LigandLigandCHHNCOOH
NOHZnO

ZnO

ZnO

NH3 plasma
treatment

Amino group
modification

Immobilizationof ligand

Fig. 1 Schematic representation of ZnO
nanoparticles
preparationforbioimaging
applications.
We have proved that surface wave plasma
excited in ammonia and mixtures of ammonia andargon successfully functionalize the surface of ZnO
nanoparticles with amine groups, and, in someconditions, it enhances their photoluminescence.
Our focus further goes to the possibility ofbiomolecule binding to the amine groups. In thisstudy we study the connection of sugar chain(dextran) to amine groups introduced on the ZnO
nanoparticles by means of fluorescence andphotoluminescence measurements.
We tested the possibility of sugar chain
connection using two methods: connection offluorescence labeled dextran, and fluorescence
detection of dextran’s hydroxyl groups after thereaction of unlabeled dextran with the aminefunctionalities on the nanoparticles. In the first
method, fluorescent dye labeled dextran(fluorescein isothiocyanate-dextran – FTIC-dextran,40.000 Da) is first oxidized and then reacts withplasma treated ZnO NPs samples. The substrates
with deposited and aminated ZnO NPs arevisualized using fluorescence microscope; in thesame time, equal quantities of NPs are removed
from the substrates and analyzed in liquid byfluorescence spectrophotometer for quantificationof fluorescence.
For the second evaluation method dextran(unlabeled) is firstly oxidiezed, then reacts with thesamples. Hydroxyl group specific fluorescent dye(5-(4,6-Dichlorotriazinyl) Aminofluorescein –5-DTAF) is used to detect detran’s free hydroxyl
groups. The detection is performed in the similar
way as for the FTIC-dextran. Fig. 2 shows thecomparison between the results of the two methods,
visualized at fluorescent microscope for untreatedsamples and for plasma aminated samples,respectively. It is clear that in both methods dextran
is successfully connected to the amine groups
introduced on the surface of the nanoparticles byplasma processing. Details about quantification offluorescence in different processing conditions will
be presented in the conference.

Fig. 2 Fluorescence microscope images of
untreated and plasma aminated ZnO after reaction
with dextran using two methods.