S007E

Contract analysis HPLC ＆ Mass spectrometry
RP-HPLC Mass Spectrometric analysis (P/N: LCMS-11)
Samples are separated by the high resolution reverse phase columns, which wehave developed and mass spectrometric analyses can be performed by the useof an ion trap instrument.
Results can be offered: RP-HPLC analysis and MS data (the major peak).
Required amount of sample: about 0.5 mg ~Lead time: basically 5 working days, reporting by e-mail.
Options: Data-reports in printed materials or CD-ROM
 Discussion/consultation concerning the results (within 30 days after reports
at HiPep Laboratories, max. 2 hours)
 MS for non-major peaks MS/MS analysis (partial sequence information/data base search)
Instruments: Agilent 1100 (Agilent Technologies, Inc.), Prominence(Shimadzu), HCT ultra or HCT plus (Bruker daltonics K.K.)
Column: HiPep-Cadenza C18 or HiPep-Intrada (HiPep Laboratories)

Agilent 1100 with HCT ultra

MALDI-TOF-MS analysis（P/N: MALDI-21）
Required amount of sample: about 0.5 mg ~Lead time: basically 5 working days, reporting by e-mail.
Available Options: Data-reports in printed materials or CD-ROM
 Discussion/consultation concerning results (within 30 days after reports atHiPep Laboratories, max 2 hours)
 MALDI-TOF-MS/MS (P/N: MALDI-21a): desired parent ion canbe further analyzed.
 Required amount of sample: 0.5 mg ~, Lead time: basically 5 working days
 Detailed MS/MS analysis with high energy CID* (P/N: MALDI-21b)
 *discrimination of Leu and Ile can be performed by high-energy CID, alsoamino acid analysis (separate service). Lead time: basically 5 working days
as above.
 MS/MS sequence homology search (from protein database)(P/N: MALDI-21c) Lead time as above
Instruments: ultraflex III (Bruker daltonics K.K.)

Prominence with HCT plus

[Literatures to indicate our activities] upon requests reprints are available
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Ultraflex III

Nokihara, K., Kasama, T., Tominaga, Y., Kitagawa, A., Hirata, A., Ohyama, T. and Yazawa, I., Amino Acids 48 (2016)2491-2499.
Nokihara, K., Tominaga, Kitagawa, A., Hirata, A. and Kasama, T. Peptide Science 2015, H. Hojo, T. Inazu, H. Katayama(eds.) The Japanese Peptide Society, pp 69-72, 2016.
Hirata,A., Nokihara, K., Kawamoto, Y., Bando, T., Sasaki, A., Ide, S. Maeshima, K, Kasama, T., and Sugiyama, H., J. Am.
Chem. Soc. 136 (2014) 11546-11554.
Nokihara, K., Yajima, S., Hirata, A., Sogon, T, and Yasuhara, T., FEBS Lett 587 (2013) 673-676.

General conditions for analyses Lead time will be changed due to an options, contracts status, connective holidays etc.
 All information regarding the sample including the analytical results will be handling as confidentially.
 Conditions of analysis are followed by our regulations (see home page). Unless without written agreement allcontracts are carried out under confidentiality, however individual contracts on our routine service arecharged for lawyers supervision fee.
 Basically, we will discard the rest of the samples, although returning of the remaining samples will becharged for additional expenses for packing and returning.
 We accept consultation of the resulted data and advising for further R and D methods.
 The amounts of samples described above are basically based on our experiences. It will be possible toanalyze even smaller amount. Please consult us. (GMP, GLP analyses are not allowed)

HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto, 602-8158, Japan
Tel +81-75-813-2101 E-mail: PepTenChip@hipep.jp URL: http://www.hipep.jp/