 Analysis of tissue samples using transmittance spectra The method of considering the differences of dying conditions
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TAO Natural Vision Akasaka R. C., Tokyo Inst. of Tech. Imaging Sci. & Eng. Lab.*,Tokyo Inst. of Tech. Frontier Collaborating R.C.**, Tokyou medical school***
K.Fujii , M.Yamaguchi*, N.Ohyama**, and K. Mukai***[[[\]^]

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sample B ¤sample A4&abtobÏÐ^tÒnFig.3(a)[Á c H , c E ÏÐ4Fig.3(b)[ÝÞ§ÕÁc H ' , c E 'xAbsorbanceÊÏÐk|oFig.4 [4Î()}Ù gxy^*ë Ex. 14ËÌ4Í gxy^*ë Ex. 2~^t4qqqÕÖ^ì¯oÊÏÐÒ +);k|oÕÖ^ì¯o[4Ex.1 ^t[ Hematoxyoin ^4Ex.2 ^t[ Eosin ^,»tìö4 -.§g/koÛ0ÏÐ[4^¬­®¯_`$kopq#¨§Ò1tìö4_`^opq ¼§ÒnÏÐk|oq23oI (,x: nucleus: cytoplasm
I0 (,x0Fig.1 Illustration of section of tissue sample.

400

Wavelength 700 (nm)Fig.2 Absorbance of Hematoxylin
and Eosin pigments.

Sample A

Sample A
Sample B

200CEEx. 2
100
0100

CH

Ex.1
300

(a) Distribution of CH and CE.

Sample B

200CE’
Ex.2
100
0100

Ex.1
CH ’

300

(b) Distribution of CH’ and CE’.

Fig.3 Pigment quantity estimation of sample A and sample B.

Ex. 2 Simulated

1.0TransmittanceEx. 2Original

0.00

Ex. 1
Original
Ex. 1
Simulated

500600
Wavelength700(nm)Fig.4 Simulated results of transmittance spectra
under better dyeing condition.
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