Scan the QR-Code to see our product catalogueof Cell Growth Media.

OD2 Solution Media
Structural studies on proteins using nuclear magnetic resonance (NMR) require labelling of these molecules with stable isotopes. Organisms such as bacteria and yeast are used to express the proteins.
The Silantes OD2 Solution Medium is the high-performance line amongst the range of Silantes stable
isotope-labelled cell growth media for the expression of stable isotope-labelled proteins.

Ready-to-Use Rich Growth Medium
Silantes OD2 media are rich growth media solutions that are sterile and ready-to-usefor fermentation purposes. The media are made from isotopically labelled bacterial hydrolysate and contain primarily amino acids, some low molecular weight oligopeptides
and almost no carbohydrates. The bacterial strain used is a chemolithoautotrophic organism grown on isotopically labelled inorganic substrates.

Available in Multiple Variations, Tailored to Your Needs
Silantes OD2 Solution Media are pre-adapted for E.coli or for yeast. They are available
as solution in standard pack sizes (100 mL, 200 mL, 500 mL, 1 L) or in bulk sizes. The
media are available in all combinations of the isotopic labels 2H, 13C and 15N. To see thefull product portfolio, scan the QR-code in the top-right corner.

Excellent Performance, especially in D2O
The Silantes OD2 solution media are developed for a high-performance expression of stable isotope labelled proteins. The media are adjusted in salt conditions
formulated for a > OD600 2 cell density. They can be used to grow organisms in
H2O or D2O, the latter with a 4-fold delay.
Figure 1 shows the growth curve ofE.coli in 2H-labelled Silantes OD2 Solution Medium for E.coli” and in 1 %Figure 1: Growth of E.coli 2H-labelled Silantes media
solution and in Silantes powder media in D2O

unlabelled Silantes SILEX Powder
Medium for E.coli in D2O. Figure 1
shows that both media have thesame excellent performance in D2Oand exceed OD 2.5 in less than 24hours.

www.silantes.com|

www.silantes-shop.com|

0049 (0)89 500 941 0|

info@silantes.com

Scan the QR-Code to see our product catalogueof Cell Growth Media.

OD2 Solution MediaNo Additional 13C-Glucose NeededSilantes OD2 Media are prepared from uniformly labelled bacterial cell hydrolysate of

Ralstonia eutropha. No additional 13C-glucose is needed to express 13C-labelled proteins witha high degree of efficiency.

Cost-Effective
Figure 2 and 3 show SDS-PAGEs as an example of protein expressions using
M9 medium and Silantes OD2 Solution Medium. The proteins are ubiquitin
and RNA polymerase α-subunit. The figures show the enhanced yield using
Silantes OD2 Solution Medium.

Figure 2: SDS-Page Ubiquitin

Figure 3: SDS-Page RNA Polym. α-subunit

High Standards in Quality Control
Silantes media are tested for reproducibility of the fermentation results.
Moreover, each batch is adjusted to yield the same cell density. The isotopic
enrichment of > 98 % is validated by mass spectrometry and the biologicalcompetence by growth tests. All test results are included with each delivery.

Request for a free Media sample at sales@silantes.com!
Silantes also offers stable isotope labelled reagents for M9 media such as 13C-glucose, D2O and 15NH4Cl.
Have a look at the currently available Silantes products and services at www.silantes-shop.com

www.silantes.com|

www.silantes-shop.com|

0049 (0)89 500 941 0|info@silantes.com