Title

Citation

Random PCR-based genotyping by sequencing
1technology GRAS-Di (genotyping by random ampliconsequencing, direct) reveals genetic structure ofMol Ecol Resour. 19:1153-1163. (2019)mangrove fishes

Species

Goal of the study

F. amboinensis

GRAS-Di method is applied to ecological studies where a large number ofL. fulvus

SNPs is required, such as population structure analysis of marine fish,

Z. dunckeri

while high quality DNA samples are not always available
The identification of individuals in the Tsushima leopard population isdifficult using microsatellite marker analysis due to the population’s low
genetic diversity, so more DNA markers must be developed to design an2

Genetic Diversity and Genetic Structure of the Wild
Tsushima Leopard Cat from Genome-Wide Analysis

Animals (Basel). 10:1375. (2020)

Wild Tsushima Leopard Cat

appropriate conservation program
To identify SNPs using genome-wide analysis with the GRAS-Di approachthat would clarify the genetic diversity and structure of the wild Tsushima
leopard cat.

East Asian origin of theEast Asian origin of the widespread alpine snow-bed3 herb, Primula cuneifolia (Primulaceae), in the northernwidespread alpine snow-bed
J Biogeogr. 00: 1– 13. (2020)

Pacific region

herb, Primula cuneifolia(Primulaceae)(P. cuneifolia

To explore the molecular evidence for the recent northward migration ofalpine snow-bed species in the northern Pacific region.

and its sister species)

Divergence and introgression in small apes, the genus
4 Hylobates, revealed by reduced representation

bioRxiv 2020.05.31.126078. (2020)

sequencing

Gibbons in the genus

To uncover the divergence history and the contributions of hybridisation

Hylobates

and introgression to the evolution of Hylobates

Developped a high-density linkage map and identify quantitative trait loci(QTLs) for recombinant inbred lines of Aegilops tauschii , a D-genome
GRAS-Di system facilitates high-density genetic map
5 construction and QTL identification in recombinant

Nature Scientific Reports (2020)10:21455

inbred lines of the wheat progenitor Aegilops tauschii

Recombinant inbred lines ofdonor of bread wheat, using the recently developed genotyping by Random

the wheat progenitor

Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming

Aegilops tauschiiof the large and complicated genome and generates a large number ofgenetic markers. The deduced linkage groups based on the GRAS-Di
genetic markers corresponded to the chromosome number of Ae. tauschii .
In this study, we tested the utility of GRAS-Di for genetic analysis in a

Genetic Dissection of a Precocious Phenotype in Male
6 Tiger Pufferfish (Takifugu rubripes ) using Genotyping
by Random Amplicon Sequencing, Direct (GRAS-Di)

cultured population of the tiger pufferfish Takifugu rubripes. The geneticMarine Biotechnology. doi:

Male Tiger Pufferfish

analyses included family structure analysis, genetic map construction, and10.1007/s10126-020-10013-4(Takifugu rubripes )quantitative trait locus (QTL) analysis for the male precocious phenotype
using a population consisting of four full-sib families derived from agenetically precocious line.

TitleCitation

Species

Goal of the study
Seed size and shape traits are important determinants of seed yield andA Major and Stable Quantitative Trait Locus qSS2 for
7 Seed Size and Shape Traits in a Soybean RIL
Population

QTL analysis of crown gall disease resistance in apple:
8 first plant R gene candidates effective against
Rhizobium rhizogenes (Ti)Recombinant inbred line (RIL) appearance quality in soybean [Glycine max (L.) Merr.]. Understanding theFront. Genet., 16 April 2021 |population developed fromgenetic architecture of these traits is important to enable their genetic

doi.org/10.3389/fgene.2021.646102

K099 (small seed size) ×

improvement through efficient and targeted selection in soybean breeding,Fendou 16 (large seed size)and for the identification of underlying causal genes.

Tree Genetics & Genomes volume 17,Article number: 25 (2021)

Full-sib F1 population derived
from a ‘JM7’ (susceptible) ×Sanashi 63 (resistant)

In this study, we used a full-sib F1 population derived from a ‘JM7’(susceptible) × Sanashi 63 (resistant) cross to identify quantitative traitloci (QTLs) that control resistance to tumour-inducing Rhizobium isolates
found in apple production areas in Japan.

F1 population of the resistant
Biological and molecular characterization of linalool9 mediated field resistance against Xanthomonas citri

Tree Physiol. tpab063 (2021)

subsp. citri in citrus trees.

Ponkan mandarin andQuantitative trait locus (QTL) analysis revealed that linalool accumulationsusceptible ‘Harehime’ (‘E-and Xcc (citrus canker disease, caused by the bacterial pathogen

647’ × ‘Miyagawa-wase’)

Xanthomonas citri subsp. Citri) susceptibility QTLs overlapped.

cultivar
The seedlings of hybrid
parents (N. suaveolens and N.

Analysis of the possible cytogenetic mechanism for
10 overcoming hybrid lethality in an interspecific cross

Sci Rep. 11:7812 (2021)

between Nicotiana suaveolens and Nicotiana tabacum .

tabacum), two lethal hybrid
seedlings, and one hybrid
seedling that overcame

Finding hybrids that overcome lethality, either spontaneously or by artificial
culture, lack the distal part of the Q chromosome, a region that includes
the gene responsible for lethality.

lethality.
Detection of an identical QTL for indices of smut disease incidence with a
Detection of a major QTL related to smut disease
11 resistance inherited from a Japanese wild sugarcane
using GRAS-Di technology.

Breeding Science.
doi:10.1270/jsbbs.20137 (2021)

markedly high LOD score at the end of linkage group 8 of ‘Yaenoushie’.
Japanese wild sugarcane

This QTL could be used for marker-assisted selection (MAS) tosignificantly improve smut disease resistance without negative effects onother agronomic traits.
( As of June/2021)