Human ANGPTL 2 Assay Kit- A New Biomarker of Heart Disease - Research Use Only -Angiopoietin-like proteins (ANGPTLs) are secretory form proteins and 7
ANGPTLs were identified. ANGPTL2 was found to affect vascular cells andmonocytes, and it have also been known that ANGPTL3 and ANGPTL4 play
important roles in lipid metabolism and AGF (Angiopoietin-like growth
factor)/ANGPTL6 plays in energy metabolism. These various biological effects ofANGPTL family are attracting attention as a new target of strategy against
lifestyle-related diseases like metabolic syndrome or cancer.
There is a report that high level of ANGPTL2 in blood was observed in individuals
with adiposity, strong insulin resistance, diabetes and arteriosclerosis.
It is also considered that ANGPTL2 autocrine/paracrine signaling can be a newfactor in accelerating heart disease development in the aging.
This kit is a quantitative ELISA kit which can measure ANGPTL2 concentration inhuman serum or plasma.
Increased ANGPTL2 activity
in pathologically stressed heart
accelerates cardiac dysfunction.

Pathological stress,
such as aging andhypertension,underlie heart failure,in part by increasing
ANGPTL2 expression
and secretion
In cardiomyocytes.

Excess ANGPTL2 causes cardiac dysfunction by inactivating
Akt-sarco (endo) plasmic reticulum Ca2+-ATPase (SERCA) 2a
signaling and impairing energy metabolism in cardiomyocytes,
promoting heart failure.
＊Cited and modified from Oike et al (Reference 2)
Immuno-Biological Laboratories Co., Ltd. Email: do-ibl@ibl-japan.co.jp URL: www.ibl-japan.co.jp

p = 0.13387654321-1 -0.5

Left Atrial Diameter : LAD(mm)log(Brain Natriuretic
Peptide : BNP)

Relationship of circulating ANGPTL2 levels to heart failure
associated clinical parametersr = 0.19300.5
1log(ANGPTL2)

1.52
Pulmonary Capillary Wedge
Pressure : PCWP (mmHg)

r = 0.268
p = 0.034
60
Percent of Ejection
Fraction : EF (%)

6560
55
50
45
40
35
30
2520-1-0.500.5
1log(ANGPTL2)

1.5

2-1-0.500.51log(ANGPTL2)

1.5

230
25205040151030
2010-1-0.5

00.5

11.5250-5log(ANGPTL2)Serum ANGPTL2 (ng / ml)

Circulating ANGPTL2 levels increase in mice following
transverse aorta constriction (TAC) surgey.
†1TAC
Sham

0.8

*0.6

ELISA for circulating ANGPTL2 protein
before surgery and at 2, 4, and 6 weeks after
surgery in sham and TAC-operated WT mice .

0.4
0.2
r = 0.324
0p = 0.012

0W

2W

4W

6W

After operation＊Cited and modified from Tian et al (Reference 1)

Reference:
1) Circulating ANGPTL2 Levels Increase in Humans and Mice Exhibiting Cardiac Dysfunction.
Tian Z, Miyata K, Morinaga J, Horiguchi H, Kadomatsu T, Endo M, Zhao J, Zhu S, Sugizaki T, Sato M,Terada K, Okumura T, Murohara T, Oike Y. Circ J. 2017 Sep 8. PMID:28890470
2) ANGPTL2 - A New Causal Player in Accelerating Heart Disease Development in the Aging.
Oike Y, Tian Z, Miyata K, Morinaga J, Endo M, Kadomatsu T. Circ J. 2017 Sep 25;81(10):1379-1385.
PMID:28867689

Human ANGPTL2 Assay Kit – IBL and related products
CodeSample
type

Product Name27745

H, M

Human ANGPTL2 Assay Kit – IBL27750

H

Human ANGPTL3 (highly sensitive)
Assay Kit – IBL

27749

H

Human ANGPTL4 Assay Kit – IBL

27410

M

Mouse angiopoietin-like 3
Assay Kit – IBL

VolumeMeasurement
Range

0.05 – 3.5
96 well
ng/mL
0.47 – 30
96 wellng/mL
23.44 – 1500
96 wellpg/mL
0.31 – 20
96 wellng/mL

Measuring samples
CultureSerumEDTAPlsma〇〇〇〇〇〇〇〇〇〇

Urine

CSF

Immuno-Biological Laboratories Co., Ltd. Email: do-ibl@ibl-japan.co.jp URL: www.ibl-japan.co.jp

Sup〇