ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩

IV-2. ศᏊ㐍໬ศ㔝࣓ࣥࣂ࣮1.
෸ᩍᤵ✄ᇉ♸ྖ 㸦⏕࿨⎔ቃ⣔㸧ᩍᤵᶫᮏဴ⏨ 㸦ඹྠ◊✲ဨ࣭⏕࿨⎔ቃ⣔㸧≉௵ຓᩍ ⚄ᕝ㱟㤿 㸦⏕࿨⎔ቃ⛉Ꮫ◊✲⛉㸧
2.

ᴫせศᏊ㐍໬ศ㔝࡛ࡣࠊ┿᰾⏕≀ࡢ୺せࢢ࣮ࣝࣉ㛫ࡢ⣔⤫㛵ಀゎ᫂࡟ྥࡅࠊ୺࡟㸱ࡘࡢࠕᰕࠖࢆタᐃࡋ◊✲ࢆ㐍ࡵ࡚࠸ࡿࠋ࠙㸯ࠚ᪂ወ┿᰾ᚤ⏕≀ࡢⓎぢ┿᰾⏕≀ࡢከᵝᛶࡢ኱㒊ศࡣ⫗║࡛ㄆ㆑ࡍࡿࡇ࡜ࡀ㞴ࡋ࠸༢⣽⬊⏕≀࡛࠶ࡿࡓࡵࠊࡇࢀࡲ࡛ࡢ◊✲࡛ࡣ┿᰾⏕≀ከᵝᛶࡢ඲యീࢆ༑ศ࡟ᢕᥱࡋ࡚࠸ࡿ࡜ࡣゝ࠸ษࢀ࡞࠸ࠋࡑࡇ࡛⮬↛⎔ቃ࠿ࡽࡇࢀࡲ࡛࡟ㄆ㆑ࡉࢀ࡚࠸࡞࠸᪂ወ┿᰾ᚤ⏕≀ࢆ༢㞳࣭ᇵ㣴ᰴ໬ࡍࡿࠋ࠙㸰ࠚ኱つᶍ㓄ิࢹ࣮ࢱゎᯒ┿᰾⏕≀ࡢ୺せࢢ࣮ࣝࣉ㛫ࡢ⣔⤫㛵ಀࢆศᏊ⣔⤫Ꮫⓗ࡟ゎ᫂ࡍࡿ࡟ࡣࠊ኱つᶍ㑇ఏᏊࢹ࣮ࢱࡀᚲ㡲࡛࠶ࡿࠋࡑࡇ࡛⣔⤫㐍໬ⓗ࡟⯆࿡῝࠸⏕≀✀ࢆ㑅ࡧࠊᇵ㣴࡜㑇ఏᏊࢹ࣮ࢱࡢྲྀᚓࢆ㐍ࡵ࡚࠸ࡿࠋࡑࡢࢹ࣮ࢱࢆᇶ࡟ࠊ኱つᶍ㓄ิࢹ࣮ࢱゎᯒࢆ⾜࠸ṇ☜࡞┿᰾⏕≀⣔⤫ࡢ᥎࠙㸱ࠚศᏊ⣔⤫ゎᯒࡢ᪉ἲㄽ◊✲ࢆ┠ᣦࡍࠋศᏊ⣔⤫ゎᯒ࡟࠾࠸࡚ࡣࠊゎᯒࡍࡿ㓄ิࢹ࣮ࢱࡢ≉㛗ࠊ౑⏝ࡍࡿゎᯒἲ࣭㓄ิ㐍໬ࣔࢹࣝ࡞࡝࡟ࡼࡾࠊ⣔⤫᥎ᐃ࡟೫ࡾࡀ⏕ࡌࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿࠋࡇࢀࡲ࡛ࡢ᪉ἲㄽࡣ༢୍㑇ఏᏊࢹ࣮ࢱ࡟ᇶ࡙࠸࡚◊✲ࡉࢀ࡚ࡁࡓࡀࠊ」ᩘ㑇ఏᏊ࠿ࡽᵓᡂࡉࢀࡿ኱つᶍ㓄ิࢹ࣮ࢱࢆゎᯒࡍࡿࡓࡵࡢ᪉ἲㄽࡢ᳨ウࡣࡑࢀ࡯࡝㐍ࢇ࡛࠸࡞࠸ࠋࡲࡓࠊ⌧≧࡛ࡣ㉸୪ิィ⟬ᶵୖ࡛ຠ⋡ࡼࡃసືࡍࡿゎᯒࣉࣟࢢ࣒ࣛࡶ༑ศ࡟ᬑཬࡋ࡚࠸ࡿ࡜ࡣゝ࠼࡞࠸ࠋࡑࡇ࡛ࠊ኱つᶍ㓄ิࢹ࣮ࢱゎᯒ࡟࠾࠸࡚ࡼࡾ೫ࡾࡢᑡ࡞࠸᥎ࢆ┠ᣦࡋࠊ᪉ἲㄽⓗ◊✲࡜⣔⤫ゎᯒࣉࣟࢢ࣒ࣛࡢ⣔ิ໬ࢆ⾜ࡗ࡚࠸ࡿࠋ3.

◊✲ᡂᯝ࠙㸯ࠚ኱つᶍ㓄ิࢹ࣮ࢱゎᯒ㸦㸯㸧ࢡࣜࣉࢺ⸴㢮࡟㏆⦕࡞┿᰾ᚤ⏕≀✀ࡢゎᯒ
H24 ᖺᗘࡲ࡛࡟ࣃࣛ࢜ඹ࿴ᅜࡢᾏỈࢧࣥࣉࣝ࠿ࡽ༢㞳ࡋࡓ᪂ወᚑᒓᰤ㣴ᛶ┿᰾ᚤ⏕≀

Palpitomonas bilix ࠾ ࡼ ࡧ ࢝ ࢱ ࣈ ࣞ ࣇ ࢓ ࣜ ࢫ 㢮 Roombia sp. ࡢ ⥙ ⨶ ⓗ Ⓨ ⌧ 㑇 ఏ Ꮚ㸦expressed sequence tag/EST㸧ࢹ࣮ࢱࢆྲྀᚓࡋࡓࠋࡇࢀࡽ 2 ✀࠿ࡽࡢ EST ࢹ࣮ࢱࢆඖ࡟159 㑇ఏᏊࢆྵࡴ࢔ࣛ࢖࣓ࣥࢺࢹ࣮ࢱࢆసᡂࡋࠊ᭱ᑬἲࢆࡶࡕ࠸ࡓ⣔⤫ゎᯒࢆ⾜࠺ࡇ࡜࡛Palpitomonas ࡢ⣔⤫ⓗ఩⨨ࡢゎ᫂ࢆヨࡳࡓࠋࢦࢽ࢜ࣔࢼࢫ㢮ࠊ
ᅗ 1 ࡟♧ࡋࡓ 159 㑇ఏᏊ⣔⤫ゎᯒ࡛ࡣࠊPalpitomonas ࡣࢡࣜࣉࢺ⸴㢮ࠊ࢝ࢱࣈࣞࣇ࢓ࣜࢫ㢮࡜༢⣔⤫࡜࡞ࡾࠊࡑࡢࣈ࣮ࢺࢫࢺࣛࢵࣉἲ࡟ࡼࡿࢧ࣏࣮ࢺ್ࡣ 90%௨125ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩ୖ࡜࡞ࡗࡓࠋ
ࡇࡢࢢ࣮ࣝࣉෆ࡛ࡲࡎ Palpitomonas㸦ࣁࣉࢺ⸴㢮㸧‫ۻ‬ࡀศᒱࡋࠊࡘ࠸࡛࢝ࢱࣈࣞࣇ࢓ࣜࢫ㢮ࡀ㸦ࢸࣟࢿ࣐㢮㸧ศᒱࡋࡓᚋࠊࢡࣜࣉࢺ⸴㢮࡜ࢦࢽ࢜ࣔࢼ‫ۻ‬㸦⥳Ⰽ᳜≀㢮㸧ࢫ㢮ࡀศᒱࡋࡓ࡜᥎ࡉࢀࡓࠋᡃࠎࡣࡇࡢ⏕≀⩌ࢆ͆ࢡࣜࣉࢳࢫࢱ⏕≀⩌͇࡜࿧ࡪࡇ࡜ࢆᥦၐࡍࡿࠋ⯆࿡῝࠸ࡇ࡜࡟ࠊ159 㑇ఏᏊゎᯒ࡛ࡣ‫ۻ‬㸦⣚⸴㢮㸧ࢡࣜࣉࢳࢫࢱ⏕≀⩌ࡣࠊゎീᗘࡣ࡞࠸ࡶ‫ۻ‬㸦⅊Ⰽ⸴㢮㸧ࡢࡢගྜᡂࢆ⾜࠺ࣁࣉࢺ⸴㢮ࠊ⥳Ⰽ᳜≀㢮ࠊ⅊Ⰽ⸴㢮࡜㏆⦕࡛࠶ࡿྍ⬟ᛶࡀ♧၀ࡉࢀ࡚࠸ࡿࠝᅗ 1ࠞࠋᚑࡗ࡚ࠊࢡࣜࣉࢳࢫࢱ⏕≀⩌ࡢඹ㏻♽ඛࡣගྜᡂᛶ࡛࠶ࡾࠊ

Palpitomonasࠊࢦࢽ࢜ࣔࢼࢫ㢮ࠊ࢝ࢱࣈࣞࣇ࢓ࣜࢫ㢮ࡣ஧ḟⓗ࡟ගྜᡂ⬟ຊࢆኻࡗࡓྍ⬟ᛶࡀ࠶ࡿࠋࡇࡢྍ⬟ᛶࢆ᳨ドࡍࡿࡓࡵࠊPalpitomonasࠊGoniomonassp.ࠊRoombia sp.ࡢ EST ࢹ࣮ࢱෆ࡟ⴥ⥳య㛵㐃㑇ఏᏊ㌿෗⏘≀ࢆ᥈⣴ࡋࡓࠋᮏゎᯒ࡛ࡣ࡜ࡃ࡟࢖ࢯࣉࣞࣀ࢖ࢻྜᡂ⣔࡟ࡘ࠸࡚╔┠ࡋࡓࠋ୍⯡࡟ගྜᡂ┿᰾⏕≀ࡣࠊⴥ⥳య࡟ᒁᅾࡍࡿ㠀࣓ࣂࣟࣥ⏘㸦MEP㸧⤒㊰࡛࢖ࢯࣉࣞࣀ࢖ࢻࢆྜᡂࡍ
ᅗ 1㸸159 㑇ఏᏊࢹ࣮ࢱ࡟ᇶ࡙ࡃ᭱ᑬ⣔⤫ᶞ㸬㉥ࡃࢩ࢙ ࡿࠋࡇࢀࡲ࡛ࡢ◊✲࡛ 2 ḟⓗ࡟ගྜᡂ⬟࣮ࢻࢆ࠿ࡅࡓࢡ࣮ࣞࢻࡣ͆ࢡࣜࣉࢳࢫࢱ⏕≀⩌͇࡛࠶ࡿࠋගྜᡂᛶ⏕≀⩌࡟ࡣᫍ༳ࢆ௜ࡅࡓࠋຊࢆኻࡗࡓ⏕≀✀㸦౛࠼ࡤࣃ࣮࢟ࣥࢧࢫ

㢮㸧࡛ࡶࠊMEP ⤒㊰ࢆᵓᡂࡍࡿ㑇ఏᏊࢆಖᣢࡋ࡚࠸ࡿࡇ࡜ࡀศ࠿ࡗ࡚࠸ࡿࠋࡋ࠿ࡋ

PalpitomonasࠊGoniomonas sp.ࠊRoombia sp.ࡢ EST ࢹ࣮ࢱෆ࡟ࡣ MEP ⤒㊰㛵㐃㑇ఏᏊ㌿෗≀ࡣⓎぢ࡛ࡁࡎࠊᮏゎᯒ࡛ࡣ 2 ḟⓗ࡟ගྜᡂ⬟ຊࢆኻࡗࡓ࠿࡝࠺࠿ุ᩿ࡍࡿࡇ࡜ࡣ࡛ࡁ࡞࠿ࡗࡓࠋ
ᅗ 2㸸ࢡࣜࣉࢺ⸴㸦ᕥୖ㸧ࠊࢦࢽ࢜ࣔࢼࢫ㢮㸦ྑୖ㸧࠾ࡼࡧ࢝ࢱࣈࣞࣇ࢓ࣜࢫ㢮㸦ᕥୗ㸧ࡢᑕฟ⿦⨨࡜ࢡࣜࣉࢳࢫࢱ⏕≀⩌࡟࠾ࡅࡿᑕฟ⿦⨨ࡢ㐍໬㸦ྑୗ㸧㸬ගᏛᚓ㢧ᚤ㙾෗┿ࡣ▮྿ᙯ᠇༤ኈ࠿ࡽᥦ౪ࡉࢀࡓ㸬ࢡࣜࣉࢺ⸴ᑕฟ⿦⨨ࡢ㟁Ꮚ㢧ᚤ㙾෗┿㸦ᕥୖ㸧ࡣ Hausmann㸦Int Rev Cytol
1948 52:197-276㸧ࡼࡾࠊࢦࢽ࢜ࣔࢼࢫ㢮ᑕฟ⿦⨨ࡢ㟁Ꮚ㢧 ᚤ 㙾 ෗ ┿ 㸦 ྑ ୖ 㸧 ࡣ Mignot 㸦 J Microscopie 1965126ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩

4:239-252㸧ࡼࡾࠊ࢝ࢱࣈࣞࣇ࢓ࣜࢫ㢮ᑕฟ⿦⨨ࡢ㟁Ꮚ㢧ᚤ 㙾 ෗ ┿ 㸦 ᕥ ୗ 㸧 ࡣ Lee & Kugrens 㸦 J Phycol 1991
27:505-513 㸧 ࠾ ࡼ ࡧ Okamoto & Inouye 㸦 Protist 2005
156:163-179㸧ࡼࡾᢤ⢋ࡋࡓ㸬ࡇࢀࡲ࡛ࡢ㢧ᚤ㙾ほᐹ࠿ࡽࢡࣜࣉࢺ⸴㢮ࠊࢦࢽ࢜ࣔࢼࢫ㢮ࠊ࢝ࢱࣈࣞࣇ࢓ࣜࢫ㢮ࡣࠊ㟁Ꮚ㢧ᚤ㙾ほᐹ࡟ࡼࡿ࡜ࠕࢺ࢖ࣞࢵࢺ࣮࣌ࣃ࣮≧ࠖ࡟ぢ࠼ࡿᑕฟ⿦⨨㸦ejectosomes㸧ࢆࡶࡘࡇ࡜ࡀศ࠿ࡗ࡚࠸ࡿࠝᅗ 2ࠊᕥୖࠊᕥୗࠊྑୖཧ↷ࠞࠋࢡࣜࣉࢺ⸴࡛ࡣࡑࡢᵓᡂࢱࣥࣃࢡࢆ
ࢥ࣮ࢻࡍࡿ tri 㑇ఏᏊ⩌ࡀྠᐃࡉࢀ࡚࠸ࡿ㸦Yamgishi et al. J Mol Evol 2012 74:147-157㸧ࠋ⯆࿡῝࠸ࡇ࡜࡟ tri 㑇ఏᏊ㌿෗≀ࡀ Goniomonas sp.࠾ࡼࡧ Roombia sp.ࡢ EST ࢹ࣮ࢱ୰࡟Ⓨぢࡉࢀࡓࠋ୍᪉ Palpitomonas ⣽⬊࡟ࡣᑕฟ⿦⨨ࡣ࡞ࡃࠊEST ࢹ࣮ࢱ࡟ࡶ tri 㑇ఏᏊ㌿෗≀ࡣ᳨ฟࡉࢀ࡞࠿ࡗࡓࠋᑕฟ⿦⨨ࡢ᭷↓ࢆࠊ159 㑇ఏᏊ⣔⤫ゎᯒ࠿ࡽ᥎ࡉࢀࡓࢡࣜࣉࢳࢫࢱ⏕≀⩌ࡢศᒱ㡰࡟࠶࡚ࡣࡵࡿ࡜ࠊPalpitomonas ࡀศᒱࡋࡓᚋࠊࢡࣜࣉࢺ⸴㢮ࠊࢦࢽ࢜ࣔࢼࢫ㢮ࠊ࢝ࢱࣈࣞࣇ࢓ࣜࢫ㢮ࡢඹ㏻♽ඛࡀᑕฟ⿦⨨ࢆ⋓ᚓࡋࡓ࡜ゎ㔘࡛ࡁࡿࠝᅗ 2ࠊྑୗࡢᶍᘧᅗཧ↷ࠞࠋ

Palpitomonas࣭Roombia ➼ࢡࣜࣉࢳࢫࢱ⏕≀⩌࡟ྵࡲࢀࡿᚑᒓᰤ㣴ᛶ┿᰾ᚤ⏕≀࡟㛵ࡍࡿ◊✲ࡣࠊ⛉Ꮫ◊✲㈝⿵ຓ㔠ᇶ┙◊✲㸦B㸧ࠕࣁࣉࢺ࣭ࢡࣜࣉࢺ⸴㢮ࢆྵࡴ᪂ወᕧ኱⏕≀⩌ࡢᥦၐ࡜ࢡ࣒ࣟ࢔࣮ࣝ࣋࢜ࣛࢱ௬ㄝࡢ᳨ドࠖ㸦௦⾲࣭✄ᇉ㸹ㄢ㢟␒ྕ 21370031㸧ࡢᨭ᥼ࢆ࠺ࡅࡓࠋ
㸦㸰㸧᪂ወ┿᰾⏕≀✀ Tsukubamonas globosa ࡢゎᯒ⟃ Ἴ኱ Ꮫᵓෆ ࡢර ኴ㑻ụ ࠿ࡽ ༢㞳ࡉ ࢀࡓ ᪂ወᚑ ᒓᰤ 㣴ᛶ┿ ᰾ᚤ ⏕≀ Tsukubamonas

globosa ࡣࠊ࢚ࢡࢫ࢝ࣂ࣮ࢱ⏕≀⩌ࡢࢹ࢕ࢫࢥࣂ⏕≀⩌࡜࿧ࡤࢀࡿࢢ࣮ࣝࣉ࡟ྵࡲࢀࡿࠋ
ᡃࠎࡣ H23 ᖺᗘࡲ࡛࡟ Tsukubamonas ࡢ EST ゎᯒࢆ⾜࠸ࠊࡑࡢࢹ࣮ࢱࢆඖ࡟ 157 㑇ఏᏊࢹ࣮ࢱࢆసᡂࡋ኱つᶍ⣔⤫ゎᯒࢆ⾜ࡗࡓࠋ157 㑇ఏᏊࢹ࣮ࢱ࡟ࡶ࡜࡙ࡃ᭱ᑬἲ࡟ࡼࡿศᏊ⣔⤫ゎᯒ࡛ࡣࠊTsukubamonas ࢆྵࡴࢹ࢕ࢫࢥࣂ⏕≀⩌ࡀ༢⣔⤫࡜࡞ࡾࠊࡇࡢ㛵ಀࡣࣈ࣮ࢺࢫࢺࣛ
ࢵࣉ್ 92%࡛ᨭᣢࡉࢀࡓࠋࡲࡓࡇࡢࢡ࣮ࣞࢻ୰࡛ࠊTsukubamonas ࡣࡇࢀࡲ࡛▱ࡽࢀ࡚࠸ࡿ᪤▱ࡢࢹ࢕ࢫࢥࣂࢢ࣮ࣝࣉ࡟ᒓࡍࡿࡇ࡜ࡣ࡞࠿ࡗࡓࡀࠊ࣊ࢸࣟࣟ࣎ࢧ㢮ࠝᅗ 3 ࡛ࡣ Naegleriaࠞ࡜࣮ࣘࢢࣞࣀࢰ࢔㢮ࠝᅗ 3 ࡛ࡣ Diplonema ࡜ Euglenaࠞࡢᇶ㒊࠿ࡽศᒱࡋࠊࡑࡢ⣔⤫㛵ಀࡣࣈ࣮ࢺࢫࢺࣛࢵࣉ್ 99%࡛ᨭᣢࡉࢀࡓࠋᚑࡗ࡚௒ᅇࡢ 157 㑇ఏᏊ࢔ࣛ࢖࣓ࣥࢺࡢ⣔⤫ゎᯒ࡟ࡼࡾࠊ

Tsukubamonas ࡣࠊձࢹ࢕ࢫࢥࣂ⏕≀⩌ࡢ⊂❧ࡋࡓ᪂ወ⣔⤫⩌࡛࠶ࡿࡇ࡜ࠊղ࣊ࢸࣟࣟ࣎ࢧ㢮㸩࣮ࣘࢢࣞࣀࢰ࢔㢮࡜ጜጒ⩌࡛࠶ࡿ࡜᥎ ࡉࢀࡓࠋ127

ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩

ᅗ 3㸸
157 㑇ఏᏊ࢔ࣛ
࢖࣓ࣥࢺ࠿ࡽ᭱ᑬἲ࡟ࡼࡾ᥎ࡉࢀࡓ┿᰾⏕≀ࡢ⣔⤫㛵ಀࢹ࢕ࢫࢥࣂ⏕≀⩌ࡢ࠺ࡕࣖࢥࣂ㢮ࡢ࣑ࢺࢥࣥࢻࣜ࢔㸦mt㸧ࢤࣀ࣒ࡣࠊ୍⯡ࡢ┿᰾⏕≀
mt ࡟ࡃࡽ࡭ࡼࡾከࡃࡢ㑇ఏᏊࢆࢥ࣮ࢻࡋ࡚
࠸ࡿࡇ࡜࠿ࡽཎጞⓗ࡛࠶ࡿ࡜ࡶ⪃࠼ࡽࢀࠊmtࢤࣀ࣒࡜┿᰾⏕≀ࡢึᮇ㐍໬ࢆ⪃ᐹࡍࡿୖ࡛ᴟࡵ࡚㔜せ࡛࠶ࡿࠋᡃࠎࡣ Tsukubamonasࡢ mt ࢤࣀ࣒ࢆ᏶඲ゎㄞ࡟ᡂຌࡋࡓࡀࠝᅗ 4ࠞࠊ
157 㑇ఏᏊࢹ࣮ࢱ࡟ᇶ࡙ࡃ኱つᶍศᏊ⣔⤫ゎᯒ࠿ࡽゎ᫂ࡉࢀࡓࢹ࢕ࢫࢥࣂ⏕≀⩌ࡢ⣔⤫㛵ಀࢆࡶ࡜࡟ࠊࡇࡢ⏕≀⩌࡟࠾࠸࡚ mt ࢤࣀ࣒ࢥ࣮ࢻ
ᅗ 4㸸Tsukubamonas globosa ࣑ࢺࢥࣥࢻࣜ࢔ࢤࣀ࣒㑇ఏᏊࡀ࡝ࡢࡼ࠺࡟ኚ໬ࡋࡓࡢ࠿ࢆゎ᫂ࡍࡿࡇ࡜࡟ᡂຌࡋࡓࠋ

㸦㸱㸧ࣇ࢛ࣝࢽ࣮࢝ࢱ⏕≀⩌࡟ᒓࡍࡿ᎘Ẽᛶ┿᰾ᚤ⏕≀✀ࡢゎᯒࣇ࢛ࣝࢽ࣮࢝ࢱ⏕≀⩌ࡣ᎘Ẽᛶ࠶ࡿ࠸ࡣᚤዲẼᛶ┿᰾⏕≀࠿ࡽᵓᡂࡉࢀࠊ⑓ཎᛶ┿᰾ᚤ⏕≀ Giardia intestinalis ࡟௦⾲ࡉࢀࡿࢹ࢕ࣉࣟࣔࢼࢫ㢮࡜⮬⏤⏕άᛶࡢࠕ࢝ࣝ࣌ࢹ࢕࢚ࣔࢼ
ࢫᵝ⏕≀㸦Carpediemonas-like organisms or CLOs㸧ࠖࡀྵࡲࢀࡿࠋ⑓ཎᛶᚤ⏕≀࡜ࡋ࡚◊✲ࡉࢀ࡚ࡁࡓ Giardia ࡣࠊ᎘Ẽ⎔ቃ࡬㐺ᛂࡍࡿ㐣⛬࡛ዲẼᛶ mt ࢆᶵ⬟୙᫂ࡢ mt ⏤᮶࢜ࣝ࢞ࢿ࣭࣐ࣛ࢖ࢺࢯ࣮࣒࡬࡜ᨵኚࡋࡓࡇ࡜ࡀศ࠿ࡗ࡚࠸ࡿࠋࡋ࠿ࡋࠊࢹ࢕ࣉࣟࣔࢼࢫ㢮ࡢ࣐࢖ࢺࢯ࣮࣒ࡀ࡝ࡢࡼ࠺࡟ᡂ❧ࡋࡓࡢ࠿ࡣࡼࡃ⌮ゎ࡛ࡁ࡚࠸࡞࠸ࠋᡃࠎࡣࠊࣇ࢛ࣝࢽ࣮࢝ࢱ

128

ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩⏕≀⩌୰࡛ࡢ᎘Ẽᛶ mt ࠾ࡼࡧ࣐࢖ࢺࢯ࣮࣒ࡢ㐍໬ࢆゎ᫂ࡍࡿࡓࡵࠊ୺せ࡞ CLO ࠿ࡽ ESTࢹ࣮ࢱࢆྲྀᚓࡋ࡚ࡁࡓ㸦࢝ࢼࢲ࣭Dalhousie ኱Ꮫࡢ Alastair Simpson ༤ኈࠊAndrew Roger༤ኈ➼࡜ࡢඹྠ◊✲㸧ࠋࡇࢀࡽࡢ EST ࢹ࣮ࢱࢆᇶ┙࡟ 163 㑇ఏᏊ࢔ࣛ࢖࣓ࣥࢺࢆసᡂࡋࠊ኱つᶍ⣔⤫ゎᯒࢆ⾜ࡗࡓ⤖ᯝࠊࣇ࢛ࣝࢽ࣮࢝ࢱ⏕≀⩌ࡢ୺せ⏕≀✀㛫ࡢ⣔⤫㛵ಀࢆ㧗⢭ᗘ࡛ゎ᫂ࡍࡿࡇ࡜࡟ᡂຌࡋࡓࠝᅗ 5ࠞࠋH25 ᖺᗘ࡟ࡣྛ✀ CLOs ࡢ EST ࢹ࣮ࢱ୰࡟ mt ⏤᮶࢜ࣝ࢞ࢿ࡛ࣛᶵ⬟ࡍࡿ࡜⪃࠼ࡽࢀࡿ㑇ఏᏊࢆ⥙⨶ⓗ࡟᥈⣴ࡋࠊ⣔⤫㛫࡛ࡢ mt ⏤᮶࢜ࣝ࢞ࢿࣛ
ᶵ⬟ࡢᬑ㐢ᛶ࡜≉␗ᛶࢆ᳨ฟࡍࡿࠋ

ᅗ 5㸸
163 㑇ఏᏊ࢔ࣛ࢖࣓ࣥࢺ࠿ࡽ
᭱ᑬἲ࡟ࡼࡾ᥎ ࡉࢀࡓࣇ࢛ࣝࢽ࣮࢝ࢱ⏕≀⩌ࡢ୺せ⣔⤫㛫ࡢ㛵ಀᡃࠎࡀ EST ゎᯒ࡟㛵㐃ࡋࡓ⏕≀✀࡟ࡣᫍ༳ࢆ௜ࡅࡓࠋ

࠙㸰ࠚ᪂ወ┿᰾⏕≀ࡢⓎぢH24 ᖺᗘ࡟ࡣ௨ୗࡢ᪂ወ┿᰾ᚤ⏕≀ࡢᇵ㣴ᰴࢆ᪂ࡓ࡟☜❧ࡋࡓࠋH25 ᖺᗘ࡟ࡣࠊḟୡ௦ࢩ࣮ࢡ࢚ࣥࢫࢆ⏝࠸࡚ࡇࢀࡽࡢ⏕≀✀ࡢ⥙⨶ⓗⓎ⌧㑇ఏᏊゎᯒࢆ⾜࠺ணᐃ࡛࠶ࡿࠋ㸦㸯㸧᎘Ẽᛶᚑᒓᰤ㣴ᛶࢫࢺ࣓ࣛࣀࣃ࢖ࣝ⏕≀ MAST-13ࢫࢺ࣓ࣛࣀࣃ࢖ࣝ⏕≀⩌࡟ࡣࠊᾏỈ࠿ࡽᢳฟࡋࡓ DNA ࠿ࡽ PCR ἲ࡟ࡼࡾቑᖜࡋࡓ 18S ࣜ࣎ࢯ࣮࣒ RNA 㓄ิࡔࡅ࠿ࡽᏑᅾࡀ᥎ࡉࢀ࡚࠸ࡿ⣔⤫⩌㸦㏻⛠ MAST㸧ࡀከᩘᏑᅾࡍࡿࠋᡃࠎࡣࠊࡇࡢ MAST ࢢ࣮ࣝࣉࡢ࠺ࡕ MAST-13 ࡟ᒓࡍࡿ᎘Ẽᛶᚑᒓᰤ㣴ᛶ┿᰾ᚤ⏕≀ࢆ༢㞳ࡋࠊᇵ㣴ᰴ໬࡟ᡂຌࡋࡓࠋ㹙࢝ࢼࢲ British Columbia ኱࣭㞷྿┤ྐ༤ኈ࡜ࡢඹྠ◊✲㹛㸦㸰㸧᎘Ẽᛶᚑᒓᰤ㣴ᛶ┿᰾ᚤ⏕≀ PAP020 ᰴPAP020 ᰴࡣ᎘Ẽᛶ┿᰾ᚤ⏕≀࡛࠶ࡾࠊᇵ㣴ᰴ໬ࡉࢀࠊᐇ㦂ᐊෆ࡛⥅௦ᇵ㣴ࡉࢀ࡚࠸ࡿࠋࡇࡢ⣽⬊ࡢගᏛ㢧ᚤ㙾ほᐹ࡟ࡼࡿᙧែほᐹ࡛ࡣࠊࡑࡢ⣔⤫ⓗ఩⨨࡟┠ᫍࢆࡘࡅࡿࡇ࡜ࡀ࡛ࡁ
࡞࠿ࡗࡓࠋࡲࡓ 18S rRNA 㓄ิࢆỴᐃࡋศᏊ⣔⤫ゎᯒࢆ⾜ࡗࡓࡀࠊPAP020 ᰴࡀ᪤▱ࡢ࡝ࡢࡼ࠺࡞┿᰾⏕≀࡟㏆⦕࡞ࡢ࠿඲ࡃศ࠿ࡽࡎࠊ㧗ḟศ㢮⩌࡛ࣞ࣋ࣝ඲ࡃ᪂ወ࡞⏕≀࡛࠶ࡿྍ⬟ᛶࡀ࠶ࡿࠋ㹙⟃Ἴ኱࣭▼⏣೺୍㑻༤ኈࠊⓑ㫽ᓧᚿẶ࡜ࡢඹྠ◊✲㹛㸦㸱㸧⥳Ⰽ㠴ẟ⸴㢮 TRD ࠾ࡼࡧ MRD ᰴ୍⯡࡟ගྜᡂᛶ㠴ẟ⸴㢮ࡢⴥ⥳యࡣࠕ࣌ࣜࢽࢹ࢕ࣥᆺࠖ࡜࿧ࡤࢀࠊ≉␗࡞⿵ຓⰍ⣲࣌ࣜ

129

ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩ࢹ࢕ࢽࣥࢆྵࡴࠋࡑࡢ୍᪉ࠊ୍㒊ࡢ㠴ẟ⸴㢮ࡣ⣔⤫ⓗ࡟␗࡞ࡿ┿᰾⸴㢮ࡀ⣽⬊ෆඹ⏕ࢆ㏻ࡋ࡚ⴥ⥳య໬ࡋࠊ࣌ࣜࢹ࢕ࢽࣥᆺⴥ⥳య࡜⨨᥮ࡋࡓ⤖ᯝ࡛࠶ࡿࠕ㠀࣌ࣜࢹ࢕ࢽࣥᆺࠖⴥ⥳యࢆࡶࡗ࡚࠸ࡿࠋLepidodinium ᒓ㠴ẟ⸴㢮ࡣ⥳Ⰽࢆ࿊ࡍࡿ㠀࣌ࣜࢹ࢕ࢽࣥᆺⴥ⥳యࢆࡶࡕࠊࡑࡢⴥ⥳యࡣ⥳⸴㉳※࡛࠶ࡿࡇ࡜ࡀศ࠿ࡗ࡚࠸ࡿࠋᡃࠎࡣ Lepidodinium ᒓ࡜ࡣᙧែⓗ࡟␗࡞ࡿ⥳Ⰽ㠴ẟ⸴㢮 TRD ᰴ࠾ࡼࡧ MRD ᰴࢆᇵ㣴ᰴ໬ࡍࡿࡇ࡜࡟ᡂຌࡋࡓࠋ㹙ᒣᙧ኱Ꮫ࣭ᒾ⁪ග൤༤ኈࠊ─஭༓⿱Ặ࡜ࡢඹྠ◊✲㹛࠙㸱ࠚศᏊ⣔⤫ゎᯒࡢ᪉ἲㄽ◊✲㸦㸯㸧ሷᇶ⤌ᡂࡢ೫ࡾࡀࡶࡓࡽࡍ࢔࣮ࢸ࢕ࣇ࢓ࢡࢺ୍⯡࡟ศᏊ⣔⤫ゎᯒ࡛⏝࠸ࡽࢀࡿ͆homogeneous͇ሷᇶ⨨᥮ࣔࢹ࡛ࣝࡣࠊ㓄ิ㛫࡛ሷᇶ⤌ᡂࡣ኱ࡁࡃ␗࡞ࡽ࡞࠸ࡇ࡜ࢆ๓ᥦ࡜ࡋ࡚࠸ࡿࠋࡋ࠿ࡋ⌧ᐇ࡟ࡣࠊ⏕≀✀㛫࠶ࡿ࠸ࡣྠ୍ࢤࣀ࣒ࡢ␗࡞ࡿ㡿ᇦ㛫࡛ࡶሷᇶ⤌ᡂࡀ␗࡞ࡿࡇ࡜ࡀ࠶ࡿࠋ㓄ิ㛫ࡢሷᇶ⤌ᡂࡀ኱ࡁࡃ␗࡞ࡿ
ሙྜࠊhomogeneous ⨨᥮ࣔࢹࣝࢆ๓ᥦ࡜ࡋࡓゎᯒ࡛ࡣⴭࡋ࠸ࣔࢹࣝ୙ᩚྜࡀ⏕ࡌࠊࡑࡢ⤖ᯝㄗࡗࡓ⣔⤫ᶞ㸦࢔࣮ࢸ࢕ࣇ࢓ࢡࢺ㸧࡟ᑟ࠿ࢀࡿࡇ࡜ࡀศ࠿ࡗ࡚࠸ࡿࠋࡇࡢሷᇶ⤌ᡂࡢ೫ࡾ࡟㉳ᅉࡍࡿ࢔࣮ࢸ࢕ࣇ࢓ࢡࢺࢆ㜵ࡄࡓࡵ࡟ࡣࠊ㓄ิ㛫ࡢሷᇶ⤌ᡂࡢ೫ࡾࢆྲྀࡾධࢀࡓࡼࡾ」㞧࡞͆non-homogeneous͇ࣔࢹࣝ࡟ࡼࡿ⣔⤫ゎᯒࣉࣟࢢ࣒ࣛࡢᐇ⏝໬ࡀᚲ㡲࡛࠶ࡿࠋ
H24 ᖺᗘࡲ࡛࡟ࠊሷᇶ⤌ᡂࡢ೫ࡾࡢ⛬ᗘࢆኚ࠼ࡓࢩ࣑࣮ࣗࣞࢩࣙࣥࢹ࣮ࢱࢆసᡂࡋࠊ
non-homogeneous ࣔࢹࣝゎᯒࡢ㡹೺ᛶࢆヲ⣽࡟᳨ドࡋࡓࠋࡑࡢ⤖ᯝࠊnon-homogeneousࣔࢹࣝゎᯒࡣሷᇶ⤌ᡂࡢ೫ࡾ࡟㉳ᅉࡍࡿ࢔࣮ࢸ࢕ࣇ࢓ࢡࢺࢆᢚไࡋ࠺ࡿࡇ࡜ࢆ♧ࡋࡓࠋH24ᖺᗘ࠿ࡽࡣࠊ▼ᕝዌኴ㸦⏕≀⛉Ꮫᑓᨷᚋᮇ 1 ᖺ࣭CS ᑓᨷ M1㸧ࡀ non-homongeneous ࣔࢹࣝࢆᐇ⿦ࡋࡓ⣔⤫ゎᯒࣉࣟࢢ࣒ࣛ NHML㸦Galtier & Gouy Mol Biol Evol 1998 15:871-879㸧ࢆ㉸୪ิィ⟬ᶵୖ࡛ࡢ౑⏝ࢆ๓ᥦ࡜ࡋࡓᨵኚࢆ⾜࠸ࠊࡑࡢᛶ⬟ホ౯ࢆ㛤ጞࡋࡓࠋSubtree
pruning and regrafting㸦Ⓨぢⓗᶞᙧ᥈⣴㸧ࡢ୪ิ໬ࠊᑬᗘィ⟬ࡢ୪ิ໬࡟࠾ࡅࡿ㏻ಙసᴗࡢ᭱㐺໬ࠊ」ᩘࡢึᮇᶞᙧ࠿ࡽࡢᶞᙧ᥈⣴ࡢ୪ิ໬࡞࡝ࢆ⾜ࡗ࡚࠸ࡿࠋ4.

እ㒊㈨㔠ࡢ⋓ᚓ≧ἣ¾ ᇶ┙◊✲㸦B㸧ࠕࣁࣉࢺ࣭ࢡࣜࣉࢺ⸴㢮ࢆྵࡴ᪂ወᕧ኱⏕≀⩌ࡢᥦၐ࡜ࢡ࣒ࣟ࢔࣮ࣝ࣋࢜ࣛࢱ௬ㄝࡢ᳨ド㸦ㄢ㢟␒ྕ 21370031㸧ࠖ௦⾲ࠊ◊✲ᮇ㛫㸸2009~2012஺௜Ỵᐃ㢠㸸┤᥋⤒㈝ 1,800 ༓෇㸦ᮇ㛫඲య 14,200 ༓෇㸧ࠊ㛫᥋⤒㈝ 540 ༓෇㸦ᮇ㛫඲
య 4,260 ༓෇㸧
¾ ᪂Ꮫ⾡㡿ᇦ◊✲㸦◊✲㡿ᇦᥦ᱌ᆺ㸧ࠕ࣑ࢺࢥࣥࢻࣜ࢔࣭Ⰽ⣲య௨እࡢඹ⏕࢜ࣝ࢞ࢿࣛᡂ❧㐣⛬ࡢゎ᫂㸦ㄢ㢟␒ྕ 23117006㸧ࠖ௦⾲ࠊ◊✲ᮇ㛫㸸2011~2015஺௜Ỵᐃ㢠㸸┤᥋⤒㈝ 17,100 ༓෇㸦ᮇ㛫඲య 14,200 ༓෇࣭ணᐃ㸧ࠊ㛫᥋⤒㈝ 5,130 ༓෇㸦ᮇ㛫඲య 4,260 ༓෇࣭ணᐃ㸧130ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩

5.

◊✲ᴗ⦼

㸦㸯㸧◊✲ㄽᩥ
1

Matsumoto T, Kawachi M, Miyashita H, Inagaki Y. Prasinoxanthin is absent in thegreen-colored dinoflagellate Lepidodinium chlorophorum strain NIES-1868:
Pigment composition and 18S rRNA phylogeny. Journal of Plant Research
125:705-711.

2Tsuji M, Sato M, Tanabe AS, Inagaki Y, Hashimoto T. An asynchronous parallel
genetic algorithm for the maximum likelihood phylogenetic tree search. 2012

Proceedings of 2012 IEEE Congress on Evolutionary Computation. doi:
10.1109/CEC.2012.6256560
3Ishitani Y, Kamikawa R, Yabuki A, Tsuchiya M, Inagaki Y, Takishita K. Evolutionof elongation factor-like (EFL) protein in Rhizaria is revised by radiolarian EFL
gene sequences. 2012 Journal of Eukaryotic Microbiology 59:367-373.

4Ishikawa SA, Inagaki Y, Hashimoto T. RY-coding and non-homogeneous models
ameliorate the maximum-likelihood inferences from nucleotide sequence data withparallel compositional heterogeneity. 2012 Evolutionary Bioinformatics 8:357-371.

5

Nishimura Y, Kamikawa R, Hashimoto T, Inagaki Y. Separate origins of group I
introns in two mitochondrial genes of the katablepharid Leucocryptos marina. 2012

PLoS One 7:e37307.
6

Takishita K, Kolisko M, Komatsuzaki H, Yabuki A, Inagaki Y, Cepicka I,
Smejkalova P, Silberman JD, Hashimoto T, Roger AJ, Simpson AGB. Multigene
phylogenies of diverse Carpediemonas-like organisms identify the closest relativesof 'amitochndriate' diplomonads and retortamonads. 2012 Protist 163:344-355.

7Nagayasu E, Ishikawa S, Taketani S, Ooka T, Chakraborty G, Yoshida A, Inagaki Y,Hayashi T, Maruyama H. Identification of a bacteria-like ferrochelatase gene in

Strongyloides venezuelensis, an animal parasitic nematode. PLoS One 8:e58458.
㸦㸰㸧Ꮫ఍Ⓨ⾲㸦Ⓨ⾲⪅ࡣኴᏐ㸧㸦A㸧ᣍᚅㅮ₇㸦ࡍ࡭ཱྀ࡚㢌Ⓨ⾲㸧1Yuji Inagaki㸬Genome evolution of the nitrogen-fixing cyanobacterial endosymbiont
in the diatom Epithemia turgida㸬2012 ᖺ 7 ᭶ 29 ᪥-8 ᭶ 3 ᪥ Integrated Microbial
Biodiversity Program Meeting, Canadian Institute for Advanced Research㸬Hilton
Hotel, Quebec City, Quebec, Canada㸬

2Yuji Inagaki 㸬 A model for endosymbiotic genome reduction: rhopalodiacecan
diatoms and their nitrogen-fixing organelle, spheroid body㸬㸦Oral, Invited㸧2012131

ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩

ᖺ 7 ᭶ 29 ᪥-8 ᭶ 3 ᪥ Protist2012 University of Oslo, Oslo, Norway㸬
3Yuji Inagaki, Ryoma Kamikawa, Takuro Nakayama, Goro Tanifuji, Tetsuo
Hashimoto㸬⣽⬊ඹ⏕య⏤᮶࢜ࣝ࢞ࢿࣛ㐍໬࡟㛵ࡍࡿ᪂ࡓ࡞ᒎ㛤㸦New aspects of theevolution of endosymbiotic bacterium-derived organelles㸧㸬2012 ᖺ 9 ᭶ 19-22 ᪥ ➨28 ᅇ᪥ᮏᚤ⏕≀⏕ែᏛ఍㸦JSME 2012㸧㇏ᶫᢏ⾡⛉Ꮫ኱Ꮫ㸪㇏ᶫ㸪ឡ▱㸬

4Ryoma Kamikawa, Akinori Yabuki, Tetsuo Hashimoto, Yuki Nishimura, YujiInagaki㸬᪂ወ┿᰾ᚤ⏕≀ࡀࡶࡓࡽࡍ᪂ࡋ࠸┿᰾⏕≀኱⣔⤫ീ㸦Searching for missing
pieces in natural environments: With the emphasis on the importance of culture
strains of enigmatic eukaryotes for global eukaryotic phylogeny㸧㸬2012 ᖺ 9 ᭶ 19-22᪥ ➨ 28 ᅇ᪥ᮏᚤ⏕≀⏕ែᏛ఍㸦JSME 2012㸧㇏ᶫᢏ⾡⛉Ꮫ኱Ꮫ㸪㇏ᶫ㸪ឡ▱㸬5✄ᇉ♸ྖࠊ─஭༓⿱ࠊ⚄⚄ᕝ㱟㤿ࠊ㧗ᶫ࿴ஓࠊᒾ⁪ග൤㸬㠴ẟ⸴㢮࡟࠾ࡅࡿ⥳Ⰽⴥ⥳యࡢ㉳※࡜ከᵝᛶ㸬2013 ᖺ 3 ᭶ 20 ᪥ ➨ 15 ᅇ᳜≀࢜ࣝ࢞ࢿ࣮ࣛ࣡ࢡࢩࣙࢵࣉ ࣮࢜ࣝ࢞࣍ࣝ㸪ᒸᒣ㸪ᒸᒣ㸬㸦B㸧ࡑࡢ௚ࡢᏛ఍Ⓨ⾲1✄ᇉ♸ྖࠊ℧ୗΎ㈗㸬ᨺᩓ⹸▼㇂ెஅࠊ▼ᕝዌኴࠊ⚄ᕝ㱟㤿ࠊ▮྿ᙯ᠇ࠊᅵᒇṇྐࠊ✄࡜᭷Ꮝ⹸ࡀ஫࠸࡟ጜጒ⩌࡜ࡍࡿࠕࣞࢱࣜ࢔௬ㄝࠖ࡟ᇶ࡙࠸ࡓ࢔ࢡࢳࣥࠊࢳ࣮ࣗࣈࣜࣥ࠾ࡼࡧ࣌ࣉࢳࢻఙ㛗ᅉᏊࡢ」㞧࡞㐍໬ࢩࢼࣜ࢜㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 5 ᭶ 20-25 ᪥ ᪥ᮏᆅ⌫ᝨᫍ⛉Ꮫ㐃ྜ 2012 ᖺ኱఍ ᖥᙇ࣓ࢵࢭᅜ㝿఍㆟ሙ, ᖥᙇ, ༓ⴥ㸬2✄ᇉ♸ྖࠊᶫᶫᮏဴ⏨ࠊⓑ㫽ᓧᚿࠊ୰ᒣ๛ࠊ▼⏣೺୍㑻ࠊ஭ୖ໏㸬ᶓᒣள⣖Ꮚ Ᏺᒇ⦾᫓ࠊ✄࣓ࢱࢤࣀ࣑ࢡࢫ࡛᥈ࡿᮾி‴ἢᓊࡢ⸴㢮࣭ࣉࣟࢸ࢕ࢫࢺ┦㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 5 ᭶20-25 ᪥ ᪥ᮏᆅ⌫ᝨᫍ⛉Ꮫ㐃ྜ 2012 ᖺ኱఍ ᖥᙇ࣓ࢵࢭᅜ㝿఍㆟ሙ, ᖥᙇ, ༓ⴥ㸬3⚄ᕝ㱟㤿ࠊ✄✄ᇉ♸ྖࠊᶫᶫᮏဴ⏨㸬࣮ࣟࣜࣥࢢࢧ࣮ࢡࣝᆺ」〇ἲ࡟ᇶ࡙ࡃཎすᮧ♸㈗ࠊ⚄⏕⏕≀࣑ࢺࢥࣥࢻࣜ࢔ࢤࣀ࣒ࡢࢩ࣮ࢣࣥࢫゎᯒ㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 7 ᭶ 13-15 ᪥ ᪥ᮏ⸴㢮Ꮫ఍➨ 36 ᅇ኱఍ ໭ᾏ㐨኱, ᮐᖠ, ໭ᾏ㐨㸬4⚄ᕝ㱟㤿ࠊᶫᶫᮏဴ⏨ࠊ✄✄ᇉ♸ྖ㸬Karenia ᒓ▮ᓮ⿱つࠊ⚄㠴ẟ⸴㢮ࡢ୕ḟⴥ⥳య⋓ᚓ࡟క࠺ⴥ⥳యᆺ GAPDH ࡢ㐍໬㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᖺ 7 ᭶ 13-15 ᪥ ᪥ᮏ⸴㢮Ꮫ఍➨ 36 ᅇ኱఍ ໭ᾏ㐨኱, ᮐᖠ, ໭ᾏ㐨㸬5✄ᇉ♸ྖࠊᶫᶫᮏဴ⏨ࠊ஭ୖ໏㸬ሷᇶ㓄ิ᝟ሗ࡛᥈ࡿᮾி‴ἢᶓᒣள⣖ᏊࠊᏲᒇ⦾᫓ࠊ✄ᓊࡢ┿᰾ᚤ⏕≀┦࡜ࡑࡢᏘ⠇ኚື㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 7 ᭶ 13-15 ᪥ ᪥ᮏ⸴㢮Ꮫ఍➨ 36 ᅇ኱఍ ໭ᾏ㐨኱, ᮐᖠ, ໭ᾏ㐨㸬6⚄ᕝ㱟㤿ࠊྜྷ⏣ᫀᶞࠊᖹ⨾◁ḷࠊ▼⏣೺୍㑻ࠊᶫᶫᮏဴ⏨ࠊ✄✄ᇉ♸ྖ㸬ගྜᡂ⬟㐍໬◊✲ࡢࣔࢹࣝ࡜ࡋ࡚ࡢ↓Ⰽ⌛⸴㢮 Nitzschia spp㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 7 ᭶ 13-15 ᪥ ᪥ᮏ⸴㢮Ꮫ఍➨ 36 ᅇ኱఍ ໭ᾏ㐨኱, ᮐᖠ, ໭ᾏ㐨㸬7⚄ᕝ㱟㤿ࠊᶫ
ᶫᮏဴ⏨ࠊ✄✄ᇉ♸ྖ㸬 㠴ẟ⸴ Lepidodinium EST ゎᯒ࡟ࡼࡿᯇᮏᣅஓࠊ⚄132ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩ࢾࢡࣞ࢜ࣔࣝࣇ㛵㐃㑇ఏᏊࡢ᥈⣴㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᖺ 7 ᭶ 13-15 ᪥ ᪥ᮏ⸴㢮Ꮫ఍➨ 36 ᅇ኱఍ ໭ᾏ㐨኱, ᮐᖠ, ໭ᾏ㐨㸬8✄ᇉ♸ྖ㸬⌛⸴ Epithemia turgida ⣽⬊࡟࠾ࡅࡿ❅⣲ᅛᐃࢩ࢔ࣀࣂࢡࢸࣜ࢔ඹ⏕యࡢࢤࣀ࣒ゎᯒ㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 7 ᭶ 20-21 ᪥ ➨ 1 ᅇ࣐ࢺ࣮ࣜࣙࢩ࢝ᆺ⏕≀Ꮫ◊✲఍ ᅜ❧ឤᰁ⑕◊✲ᡤ, ᮾி, ᮾி㸬9⚄ᕝ㱟㤿ࠊ✄✄ᇉ♸ྖࠊᶫᶫᮏဴ⏨㸬࣮ࣟࣜࣥࢢࢧ࣮ࢡࣝᆺ」〇ἲ࡟ᇶ࡙ࡃཎすᮧ♸㈗ࠊ⚄⏕⏕≀࣑ࢺࢥࣥࢻࣜ࢔ࢤࣀ࣒ࡢࢩ࣮ࢣࣥࢫゎᯒ㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᖺ 7 ᭶ 20-21᪥ ➨ 1 ᅇ࣐ࢺ࣮ࣜࣙࢩ࢝ᆺ⏕≀Ꮫ◊✲఍ ᅜ❧ឤᰁ⑕◊✲ᡤ, ᮾி, ᮾி㸬⚄ᕝ㱟㤿ࠊᶫᶫᮏဴ⏨ࠊ✄✄ᇉ♸ྖ㸬Karenia ᒓ10 ▮ᓮ⿱つࠊ⚄㠴ẟ⸴㢮ࡢ୕ḟⴥ⥳య⋓ᚓ࡟క࠺ⴥ⥳యᆺ GAPDH ࡢ㐍໬㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᖺ 7 ᭶ 20-21 ᪥ ➨ 1 ᅇ࣐ࢺ࣮ࣜࣙࢩ࢝ᆺ⏕≀Ꮫ◊✲఍ ᅜ❧ឤᰁ⑕◊✲ᡤ, ᮾி, ᮾி㸬
11 ஂ⡿៞ኴ㑻ࠊᖹ⃝㍤ோࠊᯇᑿᜨ᲍Ꮚࠊỿⲗⳡࠊ⚄ᕝ㱟㤿ࠊ✄ᇉ♸ྖࠊᶫᮏဴ⏨㸬Dysnectes

brevis ࣑ࢺࢥࣥࢻࣜ࢔㛵㐃ࢱࣥࣃࢡ㉁࡟࠾ࡅࡿ㍺㏦ࢩࢢࢼࣝ㓄ิࡢ᥈⣴㸬㸦࣏ࢫࢱ࣮Ⓨ
⾲㸧2012 ᖺ 7 ᭶ 20-21 ᪥ ➨ 1 ᅇ࣐ࢺ࣮ࣜࣙࢩ࢝ᆺ⏕≀Ꮫ◊✲఍ ᅜ❧ឤᰁ⑕◊✲ᡤ,ᮾி, ᮾி㸬
12 Sohta Ishikawa, Yuji Inagaki, Tetsuo Hashimoto㸬RY-coding and non-homogeneousmodels can ameliorate the maximum-likelihood inferences from nucleotide
sequence data with parallel compositional heterogeneity㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 7 ᭶29 ᪥-8 ᭶ 3 ᪥ Protist2012 University of Oslo, Oslo, Norway㸬
13 Yuki Nishimura, Ryoma Kamikawa, Yuji Inagaki, Tetsuo Hashimoto 㸬 A newstrategy of organellar genome sequencing incorporating rolling circle amplification:
protist mitochondria genomes㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᖺ 7 ᭶ 29 ᪥-8 ᭶ 3 ᪥Protist2012 University of Oslo, Oslo, Norway㸬
14 Yuki Yazaki, Ryoma Kamikawa, Tetsuo Hashimoto, Yuji Inagaki 㸬 Is thereplacement of a gene encoding plastid-targeted GAPDH on-going in thedinoflagellate genus in Karenia?㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᖺ 7 ᭶ 29 ᪥-8 ᭶ 3 ᪥Protist2012 University of Oslo, Oslo, Norway㸬
15 Ryoma Kamikawa, Yuki Nishimura, Akinori Yabuki, Martin Kolisko, Alastair G. B.
Simpson, Andrew J. Roger, Ken-ichiro Ishida, Tetsuo Hashimoto, Yuji Inagaki㸬Thephylogenetic position and mitochondrial genome sequence of the enigmatic discobid

Tsukubamonas globosa㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 7 ᭶ 29 ᪥-8 ᭶ 3 ᪥ Protist2012
University of Oslo, Oslo, Norway㸬
16 Akiko Yokoyama, Shigeharu Moriya, Yuji Inagaki, Tetsuo Hashimoto, Isao Inouye㸬
Seasonal succession of eukaryotic community detected by environmental sequencesin Tokyo Bay, Japan㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᖺ 7 ᭶ 29 ᪥-8 ᭶ 3 ᪥ Protist2012

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ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩

University of Oslo, Oslo, Norway㸬
17 Martin Kolisko, Ryoma Kamikawa, Kiyotaka Takishita, Ivan Cepicka, QianqianZhang, Tetsuo Hashimoto, Akinori Yabuki, Yuji Inagaki, Andrew J Roger, Alastair
GB Simpson㸬Evolutionary analysis of mitochondrial ancestry of diplomonads:Phylogenomics

ofmitochondrial-relatedorganellesin

Carpediemonas-likeorganisms㸬㸦ཱྀ㢌Ⓨ⾲㸧2012 ᖺ 7 ᭶ 29 ᪥-8 ᭶ 3 ᪥ Protist2012 University of Oslo,Oslo, Norway㸬
18 ⚄ᕝ㱟㤿ࠊすᮧ♸㈗ࠊ▮྿ᙯ᠇ࠊMartin KoliskoࠊAlastair GB SimpsonࠊAndrew JRogerࠊ▼⏣೺୍㑻ࠊᶫᶫᮏဴ⏨ࠊ✄✄ᇉ♸ྖ㸬The enigmatic discobid Tsukubamonas

globosa: phylogenomic analysis and mitochondrial genome sequence㸦᪂ወ༢⣽⬊┿᰾⏕≀ Tsukubamonas globosa ࡢ⣔⤫ⓗ఩⨨࡜࣑ࢺࢥࣥࢻࣜ࢔ࢤࣀ࣒㸧㸬㸦࣏ࢫࢱ࣮Ⓨ
⾲㸧2012 Dec 11-14 ➨ 35 ᅇ᪥ᮏศᏊ⏕≀Ꮫ఍ ⚟ᒸᅜ㝿఍㆟ሙ࣭࣐࣓ࣜࣥࢵࢭ⚟ᒸ㸪⚟ᒸ㸪⚟ᒸ㸬ᶫᮏဴ⏨ࠊ✄✄ᇉ♸ྖ㸬The genes encoding elongation
19 ⚄ᕝ㱟㤿ࠊすᮧ♸㈗ࠊᕥᏊⰾᙪࠊᶫ
factor 1 and elongation factor-like protein co-exist in distantly related eukaryoticgenomes㸦┿᰾⏕≀࡟࠾ࡅࡿ⩻ヂఙ㛗ᅉᏊ EF-1 ࡜ EFL ࢆ୧᭷ࡍࡿࢤࣀ࣒ࡢศᕸ㸧㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᭶ 12 ᭶ 11-14 ᪥ ➨ 35 ᅇ᪥ᮏศᏊ⏕≀Ꮫ఍ ⚟ᒸᅜ㝿఍㆟ሙ࣭
࣐࣓ࣜࣥࢵࢭ⚟ᒸ㸪⚟ᒸ㸪⚟ᒸ㸬
20 ⚄ᕝ㱟㤿ࠊMartin KoliskoࠊJan AnderssonࠊAlastair GB SimpsonࠊAndrew J Rogerࠊ✄ ᇉ ♸ ྖ ࠊ ᶫ ᮏ ဴ ⏨ 㸬 A comparative transcriptomic analysis of themitochondrion-related organelles in members of an anaerobic protist group,Fornicata㸦᎘Ẽ⎔ቃ࡟㐺ᛂࡋࡓࣇ࢛ࣝࢽ࣮࢝ࢱ⏕≀⩌࡟࠾ࡅࡿ࣑ࢺࢥࣥࢻࣜ࢔㛵㐃࢜ࣝ࢞ࢿࣛࡢࢺࣛࣥࢫࢡࣜࣉࢺ࣮࣒ẚ㍑ゎᯒ㸧㸬㸦࣏ࢫࢱ࣮Ⓨ⾲㸧2012 ᭶ 12 ᭶ 11-14 ᪥➨ 35 ᅇ᪥ᮏศᏊ⏕≀Ꮫ఍ ⚟ᒸᅜ㝿఍㆟ሙ࣭࣐࣓ࣜࣥࢵࢭ⚟ᒸ㸪⚟ᒸ㸪⚟ᒸ㸬
21 ⚄ᕝ㱟㤿ࠊMartin KoliskoࠊJan O AnderssonࠊAlastair GB SimpsonࠊAndrew J Rogerࠊ✄ᇉ♸ྖࠊᶫᶫᮏဴ⏨㸬࣑ࢺࢥࣥࢻࣜ࢔㛵㐃࢜ࣝ࢞ࢿࣛࡢ⦰㏥㐍໬㸬㸦ཱྀ㢌Ⓨ⾲㸧2012ᖺ 12 ᭶ 19-21 ᪥ ᪥ᮏ࣑ࢺࢥࣥࢻࣜ࢔Ꮫ఍➨ 12 ᅇᖺ఍ ⟃Ἴ኱Ꮫ㸪ࡘࡃࡤ㸪Ⲉᇛ㸬✄ᇉ♸ྖ㸬᎘Ẽᛶᐤ⏕ཎ⹸࡟࠾ࡅࡿ࢚ࢿࣝࢠ࣮௦ㅰ
22 ᶫᮏဴ⏨ࠊ⚄ᕝ㱟㤿ࠊᯘዉࠎᏊࠊ✄㛵㐃㓝⣲ࡢศᏊ㐍໬㸦Molecular evolution of energy metabolism-related proteins inanaerobic protozoan parasites㸧㸬㸦ཱྀ㢌Ⓨ⾲㸧2013 ᖺ 3 ᭶ 29-31 ᪥ ➨ 82 ᅇ᪥ᮏᐤ⏕⹸Ꮫ఍ ᪥ᮏ་⛉ṑ⛉኱Ꮫ㸪ᮾி㸪ᮾி㸬
6.

␗ศ㔝㐃ᦠ࣭ᅜ㝿㐃ᦠ➼

࡞ࡋ

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ᖹᡂ 24 ᖺᗘ ⟃Ἴ኱Ꮫ ィ⟬⛉Ꮫ◊✲ࢭࣥࢱ࣮ ᖺḟሗ࿌᭩

ᅜ㝿άື

7.

Councilor-at-large, International Society of Evolutionary Protistology㸦2008 ᖺࡼࡾ㸧ᩍ⫱8.
¾▮ᓮ⿱つࠊಟኈ㸦⌮Ꮫ㸧ㄽᩥ㢟┠ࠕKarenia ᒓ㠴ẟ⸴㢮ࡢ୕ḟⴥ⥳య⋓ᚓ࡟క࠺ⴥ⥳యᆺ GAPDH ࡢ㐍໬ࠖ¾ஂ⡿៞ኴ㑻ࠊᏛኈ㸦⌮Ꮫ㸧ㄽᩥ㢟┠ࠕFornicata ⏕≀ࡢᣢࡘ࣑ࢺࢥࣥࢻࣜ࢔㛵㐃ࢱࣥࣃࢡ㉁࡟Ꮡᅾࡍࡿ㍺㏦ࢩࢢࢼࣝࡢ᥈⣴ࠖ9.

♫఍㈉⊩࣭ᅜ㝿㈉⊩࡞ࡋ10. ࡑࡢ௚㛵ᮾࣉࣟࢸ࢕ࢫࢺ೜ᴦ㒊㸦┿᰾ᚤ⏕≀ࢆᮦᩱ࡟ࡋࡓᖜᗈ࠸◊✲⪅ࡢ┦஫஺ὶࢆ┠ᣦࡍ◊✲఍㸸https://sites.google.com/site/memicrobes/home/kpc㸧ࢆ୺ദࡋ࡚࠸ࡿࠋ
ͤ2012 ᖺ 6 ᭶ 1 ᪥㸦㡰ኳᇽ኱㸧࡜ 2013 ᖺ 2 ᭶ 12 ᪥㸦ᮾிឿᜨ་⛉኱㸧࡟࡚㛤ദࡋࡓࠋ135