Vol. 20, No. 2 (2021)

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2Point-of-care diagnostic tools: a look into the rapid detection ofbacteria and other potential applications············· 1Michael Muljadi, Chao-Min Cheng*2AI-2000

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4343··········· 3043Advancing 3D Organoid Culture with Macroscopic Localization ofExtracellular Matrices by the MultiGel-Device Platform
Kasinan SUTHIWANICH, Masaya HAGIWARA ··········· 32MS··········· 36··········· 4043··········· 445··········· 46··········· 48··········· 50
Microphysiological system(MPS)··········· 52··········· 54··········· 56··········· 582021

Point-of-care diagnostic tools: a look into the rapid detection ofbacteria and other potential applications
Michael Muljadi, Chao-Min Cheng*
Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan

Abstract
Bacteria such as Eschericia coli (E. coli) remain one of the major microorganisms that present challenges to publichealth, the food and biotech industry, and the environment. Efforts to battle the microorganism has been met with
continuous roadblocks and resistances such as inaccessibility to biochemical analytical tools and the emergence ofmulti-drug resistant strains, resulting in a multitude of challenges that demand serious attention. The advent of pointof-care (POC) diagnostic tools opened up doors for research that look for new strategies not only in disease
management such as bacterial infections, but also in clinical treatment strategies, environmental management, andaccessibility to modern analytical technologies in low-resource settings. Five diverse studies that looked at POC tools
to detect bacteria in a variety of applications serve as an insight to current and future potential of rapid detection
devices that could make a positive impact to human life and the world.
Keywords: Point-of-care diagnostics; Eschericia coli

1. Introduction
Bacteria

In the field of epidemiology and public health, thethat

emergence of microorganisms that are resistant toconstitute a major part of the prokaryote family. They play aare

single-celledorganisms

antimicrobials have been a source of concern for scientists

major role in the earths ecosystem and are beneficial toand medical professionals alike [8]. It was suggested that

agriculture [1], the food and biotech industry [2], and evenpersistent misuse of antibiotics could lead to much morein medicine [3]. Despite its benefits, bacteria remain an area

serious global antibacterial resistance, the burden of whichof concern in the spread of diseases [4-5], contamination ofremains hard to predict [9]. To date, it still remains one offood and water sources worldwide [6], and the environment

the major public health concerns of the century, ironically[7].

driven by easy access and its abuse on minor ailments,
especially in the developing world [10]. Antimicrobial-101, Section 2, Kuang-Fu Road, Hsinchu, Taiwan 30013 TEL: +886 (3) 5162420, FAX or Direct Dial: +886 (3) 5745454, Email: chaomin@mx.nthu.edu.twAI-2000

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Gnome Analysis and Proteome Analysis, MicroTAS,
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Bioimaging in Near InfraredKohei SOGA*1-3
1

Faculty of Advanced Engineering, Tokyo University of Science
Research Institute for Biomedical Sciences, Tokyo University of Science2(2 lines blank)

Abstract (in bold face Arial 10pt)
For bioimaging, transparency is one of the most important issues for achieving. Simply, the matter is light can
propagate straight ahead or not. If not, the object is not transparent. By using visible light, the light penetration
depth is only a few millimeters. It may be possible to make biological tissues transparent by using some sort ofchemicals for visible light, though it must kill the cells and tissues. On the other hand, the depth is in centimeterorder for near infrared light. In this paper, the method to achieve transparency for biological tissues is discussed.
On the last part of the paper, the unique nano-structure of the probe for photodynamic therapy excited by nearinfrared will be introduced as an example of nanotechnology required for the near infrared biophotonics withtransparency.
(1 line blank)
Keywords: Transparency, Bioimaging, Near Infrared, Nanostructure(1 line blank)
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Ping Liu

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Yuta Nakagawa

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Kasinan Suthiwanich RIKEN

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