化学とマイクロ・ナノシステム研究会誌
Vo.4, No.2 (2006)

化学とマイクロ･ナノシステム
第４巻 第２号

目次
細胞実験のためのマイクロシステムの開発
佐藤 記一 ･････････・・・・・･・・・・・・・・・・・・・・・

１

金ナノ粒子による目視 SNPs 識別法
佐藤 香枝 ･････････・・・・・・・・・・・・・・・・・・・・・

６

マイクロチャネルを利用した微粒子分級法の開発
関実，山田 真澄･･・・・・・・・・・・・・・・・・・・・

１１

関連学会情報･･････････・・・・・・・･･･････・・・・・・・・・・・・・・・・・・・・・・・・

１７･･･････････････････････････････・・・・・・・・・・・・・・・・・・・・・・・

２１･･･････････・・・・・・・・・・・・・・・・・・・・・････････・・・・・・・・・・・・・

２２

会費規定

変更届

化学とマイクロ・ナノシステム研究会 入会案内････・・・・・・・・・・・・・・

２３

細胞実験のためのマイクロシステムの開発
佐藤 記一*
東京大学大学院農学生命科学研究科

Development of Microsystems for Cellular Experiments
Kiichi SATO*
Graduate School of Agricultural and Life Sciences, The University of Tokyo

Abstract
Cellular experimental systems become a major trend in µ-TAS research field. Various kinds of cells were used in
the microfluidic systems, such as primary culture of mammalian cells and cell lines, bacteria, and yeast. A wide
variety of analysis systems have been reported so far, including cell sorter, cellular imaging, and bioassay systems.
Recently, parallelization by multichannel systems was also realized. These systems were superior in efficiency ofthe processes and small consumption of precious cells and expensive reagents.

金ナノ粒子による目視 SNPs 識別法
佐藤 香枝 1,2
1

理化学研究所，2 東京大学大学院工学系研究科

Non-cross-linking gold nanoparticle aggregation for sensitive
detection of single-nucleotide polymorphisms
1

Kae SATO

Bioengineering Laboratory, RIKEN
2
School of Engineering, The University of Tokyo

Abstract
Aggregation of DNA-modified gold nanoparticles in a non-cross-linking configuration has extraordinary
selectivity against terminal mismatch of the surface-bound duplex. In this paper, we demonstrate the utility ofthis selectivity for detection of single-base substitutions. The samples were prepared through standard protocols:
DNA extraction, PCR amplification, and single-base primer extension. Oligonucleotide-modified nanoparticles
correctly responded to the unpurified products from the primer extension: aggregation for the full match anddispersion for all the mismatches. Applicability of this method to genomic DNA was tested with five humantumor cell lines, and verified by conventional technologies: mass spectrometry and direct sequencing. Unlike theexisting methods for single-base substitution analysis, this method does not need specialized equipments, andopens up a new possibility of point-of-care diagnosis for single-nucleotide polymorphisms.

マイクロチャネルを利用した微粒子分級法の開発
関1

実 *1，山田

真澄 2

大阪府立大学大学院工学研究科，2 東京大学大学院工学系研究科

Development of Particle Classification Method Using Microchannels
1

Minoru Seki *1, Masumi Yamada 2

Graduate School of Engineering, Osaka Prefecture University,
2
School of Engineering, The University of Tokyo

Abstract
A size-dependent particle separation (classification) is one of the most important procedures in biochemical,environmental, or medical analyses and their applications. However, the smaller particle size or the smaller size
difference makes the particle classification difficult. The Lab-on-a-Chip concept have encouraged theminiaturization of, various separation or selection methods for small particles, including polymer beads, cells,and macromolecules. Mmicrofluidic devices have a potential to facilitate rapid and precise particle manipulation,
due to accurately fabricated structures close to particle sizes, in micrometer or sub-micrometer dimensions. In
this paper, the newly developed methods, pinched flow fractionation and hydrodynamic filtration, wereintroduced for continuous and hydrodynamic particle separation in microfluidic devices without the help of outerfields. In these methods, the hydrodynamics of laminar flow is utilized in a microchannel network, notnecessitating any outer field controls.