化学とマイクロ・ナノシステム研究会誌
Vol. 4, No. 1 (2005)

化学とマイクロ・ナノシステム
第4巻 第1号
目次

マイクロチップ電気泳動による高速光学異性体分離
北川 文彦, 大塚 浩二 ··························································

1

DNA 解析のためのナノバイオデバイスの開発
加地 範匡, 馬場 嘉信 ··························································

5トランスデューサーズ ‘05 国際会議報告
藤田 博之, 荒川 貴博, 庄子 習一,
中西 博昭, 菊谷 善国 ··························································

9［技術レポート］汎用 ELISA 装置: µEL400
渡慶次 学 ···············································································

17

平成 17 年度化学とマイクロ･ナノシステム研究会通常総会議事録·······················

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会費規程

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関連学会情報化学とマイクロ・ナノシステム研究会 入会案内····················································

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マイクロチップ電気泳動による高速光学異性体分離
北川 文彦*, 大塚 浩二
京都大学大学院工学研究科

Rapid Enantioseparation by Microchip Electrophoresis
Fumihiko KITAGAWA*, Koji OTSUKA
Graduate School of Engineering, Kyoto University

Abstract
Microchip electrophoresis (MCE) can achieve faster analysis of small volumes of samples withhigh separation efficiency. These characteristics are suitable for the enantioseparation, and thistechnique enables chiral separations to be performed in seconds on microchips. Since a
prerequisite for achieving sufficient resolution and detectability for chiral analyses is theapplication of appropriate detection methods providing sensitivity for target enantiomers with nodead volume between a separation channel and a detection site, various detection schemes havebeen introduced in the chiral MCE analysis. In the early stage of the investigation of the chiral
MCE analysis, laser-induced fluorescence (LIF) spectrometry was mainly employed as a detection
scheme. However, a drawback of the LIF detection is that most of the analytes should bederivatized prior to analysis, which is not only labor-intensive and sometimes troublesome but
also affects the chiral recognition of selectors such as cyclodextrins. Thus, other detection
methods, e.g., UV absorption, electrochemical detection, conductometry, and so forth, have been
also introduced in the MCE analysis of chiral compounds. This article gives an overview oforiginal work done in the chiral MCE analysis with regard to approaches to improve resolution
and sensitivity for enantiomers.

Keywords: Microchip Electrophoresis; Chiral Separation; Detection Method; Cyclodextrin
Electrokinetic Chromatography

DNA 解析のためのナノバイオデバイスの開発
加地 範匡*1, 馬場 嘉信 1, 21

名古屋大学大学院工学研究科, 2 産業技術総合研究所

健康工学研究センター

Development of Nanobiodevices for DNA Analysis
1

Noritada KAJI*1, Yoshinobu BABA1, 2

Graduate School of Engineering, Nagoya University
Health Technology Research Center, National Institute of Advanced Industrial Science andTechnology (AIST)

2Abstract
Nanotechnologies are making a shift in the µTAS field from microfluidics to nanofluidics andshow a great promise to generate innovative analytical techniques. In this paper, as a one ofexample of successful fusions of nanotechnologies and bioanalysis, we introduced the nanopillar
chips for DNA separation, which consist of nanopillar array structures inside a microchannel. The
nanopillar chips could apply for the separation of a wide size range of DNA molecules, from 1 to166 kbp. For further development of these nanopillar chips, various factors affecting theperformance of nanopillar chips, e.g. geometry of nanopillars and electroosmotic flows, wereinvestigated from the both sides of actual experiments and numerical simulations. Fundamental
study to elucidate a physical property of nanospace was also performed.

Keywords:Nanopillar chip; DNA; Electrophoresis; µTAS

トランスデューサーズ’05 国際会議報告
藤田 博之 1, 荒川 貴博 2, 庄子 習一 2, 中西 博昭 3, 菊谷 善国 4, 51

東京大学生産技術研究所, 2 早稲田大学理工学部,
3
島津製作所, 4 神奈川科学技術アカデミー, 5 マイクロ化学プロセス技術研究組合

13th International Conference on Solid-state Sensors, Actuators andMicrosystems
Hiroyuki Fujita1, Takahiro Arakawa2, Shuichi Shoji2, Hiroaki Nakanishi3,Yoshikuni Kikutani4, 512

Institute of Industrial Science, The University of Tokyo;
Department of Electrical Engineering and Bioscience, Waseda University;
3
Shimadzu Co.; 4 Kanagawa Academy of Science and Technology;
Research Association of Micro Chemical Process Technology［技術レポート］

汎用 ELISA 装置: µEL400
渡慶次 学
マイクロ化学技研株式会社

Microchip-Based ELISA (Enzyme-Linked Immunosorbent Assay)
System: µEL400
Manabu Tokeshi
Institute of Microchemical Technology

Abstract
A microchip-based enzyme-linked immunosorbent assay (ELISA) system, µ-, was developed. The
system was composed of a microchip with a Y-shaped microchannel and a dam structure,polystyrene beads, and a thermal lens detector. All reactions required for the immunoassay
were done automatically in the microchannel by successive introduction of a sample and reagents.
By using this system, assay time was shortened and high sensitivity analysis was realized.

Keywords: Immunoassay; ELISA; Microchip; Thermal lens detector