SOLUTION SHEETStereo µPIV system

All-in-one stereoscopic µPIV system for 2D3C measurements in micro scale

Stereoscopic µPIV system for accurate flow measurements in microscale
The all-in-one Stereo µPIV system from Dantec Dynamics comes with high magnification,high resolution, adjustable field depth control and superior measurement accuracy. A
dedicated image calibration kit improves accuracy by providing real calibration images
simulating real experimental situations. Three different illumination alternatives increase
the flexibility during experiment setup, calibration and measurements. The system issuitable both for conventional dual pulse imaging and for time resolved imaging. Inshort, the preferred solution to 2D3C velocity measurements in microscale.

Key benefits•

Accurate investigation of flows to validate CFD results or physical models•

Simple all-in-one solution – easy to handle also for new users•

Easy, flexible adjustments reduces experiment setup time•

Saves time on calculations and puts focus on the experiment•

Excellent repeatability and traceability•

Safe laboratory solution in compliance with laser safety regulations•High degree of flexibility to adapt to different measurement applications

Micro PIV and its application areas

Micro Particle Image Velocimetry (µPIV) is a mature technique within fluid
mechanics that allows for measurement with micron resolution. A conventionalµPIV system provide planar 2-component flow fields (2D2C), however, mostmicrofluidic flows are often 3D in nature.
Stereo µPIV is the variation of the technique that provide measurement of all
three-velocity components (2D3C). With the advances in research into medicine,
molecular biology and genetics, investigation of 3D fluid flows has received
increased focus.
Relevant and interesting applications include: experiments involving biologicalflows; validation of MEMS; lab-on-a-chip devices; flow focusing and separation;
cell detection and manipulation; assay implementation; DNA purification; protein
crystallization; micromixers; droplet and bubble dispensers.

Enhanced focus on microfluidics

Stereo µPIV system with a dedicated calibration kit
The Stereo µPIV system from Dantec Dynamics is the perfect solution for
performing velocity measurements in microfluidic devices. The system isoptimized for high resolution, high magnification and high measurement
accuracy. The all-in-one solution consists of:•

Stereo microscope with control unit•

Calibration kit•

LED illumination for calibration•

System computer and synchronizer•

Laser illumination for measurement•

Software (DynamicStudio)•Cameras•

Microfluidic device

Overview of a Stereo µPIV system.

To provide the planar 3C flow field a stereoscopic image calibration of the system
is required. Positioning a calibration target in a microfluidic channel is quite
challenging. Therefore, the Dantec Dynamics solution comes complete with adedicated calibration kit and dedicated illumination device that makes it possible toperform proper stereoscopic image calibration. Thanks to this unique calibration kit
the all-in-one Stereo µPIV system is the right solution for microfluidics researchers
who want the absolutely best accuracy during flow investigations in order tovalidate CFD results or physical models.

The system components
Stereo microscope
The microscope configuration is carefully selected and modified for the requirements of an accurate Stereo µPIV measurement. The microscope features fullyplan apochromatic corrected optics, 16,5:1 zoom ratio, a double-iris diaphragm tocontrol the field depth, an optional objective slider to switch to conventional 2DPage 2Calibration kit

imaging, white LED illumination, and different options for C- and F-mountcamera adapters.
The total magnification range depends on the selected camera adapter: It ispossible to get 1x-18x and 2x-36x magnification range for C-mount cameras,and 3.6x-57.6x magnification range for F-mount cameras. The magnification
can be changed continuously for flexibility or step-wise for repeatability.
A motorized hand control unit is included in the configuration for ease of use
and for display of coded functions. Zoom, iris diaphragm and the common
main objective is coded in the control unit. The coding allows accurate
documentation of magnification, field of view, depth of focus and resolution.
This means reproducibility and consistency in experimental procedures andpermits calibrated measurements at all zoom levels.

Control device with display for easy
motorized control of microscope

LED illumination
Two separate LED illumination systems are integrated in the microscopeconfiguration: A continuous white LED illumination for quick alignments and apulsed focused green LED illumination for the calibration process. The intensityof both light sources can be adjusted manually or through the software.
For slow microfluidic flows in transparent samples with less than 100mm/s
velocity, the Microstrobe LED illumination can be used in transmission mode.
This compact, dedicated, low cost, pulsed and focused LED illumination
eliminates all the laser safety requirements ensuring a completely safe
operating environment for the researcher. LED illumination also allows theinvestigations of biological flows without destroying the live organisms.
In addition to the LED illumination a number of high- and low-repetition rate
PIV lasers are compatible with the microscope. The low-repetition rate PIV
lasers can be used for measurements in steady flows, and the high-repetition
lasers can be used for time-resolved measurements of transient flows. The
light coupling is safely achieved using a liquid light guide. For a complete list ofcompatible lasers, please consult your local Dantec Dynamics representative.

CamerasWhen it comes to camera selection, Dantec Dynamics is the most flexiblechoice. With more than 100 different camera models supported, it is easy topick a suitable camera for the experiment’s spatial resolution, pixel sensitivity
and temporal resolution requirements: This camera range includes CCD, CMOS
and sCMOS detectors, from VGA to 29Mpix resolution, up to 70% absolute
quantum efficiency (QE) and up to 25600 frames per second. Different C- andF-camera mounts are available to adapt the field of view to the detector size.
Please consult your Dantec Dynamics representative for camera selection.

Calibration kit
Stereoscopic image calibration in a microchannel is simply difficult if notimpossible. In order to achieve an accurate image calibration we introduce
a dedicated image calibration kit, which consists of a calibration target
supported by the software, a calibration pool and a micro stage to traverse
the target in the calibration pool. Most importantly, the top surface of thecalibration pool and the microchannel are the same simulating similar
optical paths during calibration and measurements. The calibration target
includes two checkerboard patterns for ~1mm and ~0.5mm object size.
The checkerboard patterns on the calibration target have good contrast
with respect to the background. Carefully placed circular markers allow anunambiguous definition of the planar coordinate axes.

Page 3Checkerboard for accurate calibrationof microscope3D microchannel
The microfluidic device has three flow inlets and two regions where one
can create 3D flow fields: a 500µm-diameter hole and a 500µm-high step.
Canonical fluid flows can be observed; i.e. an impinging jet, oscillating jet or a
jet in cross-flow using the hole. Similarly, flow over backward facing step,or flow approaching a step can be measured using the 2D obstacle in the1mm-deep channel. The channel width is 5mm, which allows for an easy
optical access even with a high stereoscopic viewing angle.

Viewing 3D results
It can be challenging to find intricate flow details when one is observing acomplicated flow field. There are two standard views available to visualize
2D3C vector fields: The first is a 2D view where the u and v components are
displayed as vectors and the w component with a color map. The second is a
3D view where all three-velocity components are represented with vectors. Therotatable 3D view (bottom, right) clearly shows a vortex positioned close to
one of the corners of the micromixer (top, right).

Microfluidics 3D microchannel device

Software
The µPIV system includes a full version of DynamicStudio, which supports:•More than 100 camera models•Any illumination device through Light Source Wizard•

Distributed data acquisition and distributed data storage•Easy set-up with plug-and-play connection of cameras, timer devices andother hardware•

Image Processing Library (IPL) – a comprehensive collection of advanced
image processing functions•

Proper Orthogonal Decomposition (POD) – advanced energy based flow
decomposition•

Stability analysis using Oscillating Pattern Decomposition (OPD)•

MatLab link to perform end-user specified analysis routines scripted inMatLab•

Advanced 2D PIV algorithms: Adaptive PIV and Least Squares Matching (LSM)•Advanced 2D PTV algorithms based on 3-, and 4-frame tracking•Calculation of vector derivatives like vorticity, streamlines, and the λ2parameter•

Advanced graphical representation of the measured parameters without
requiring additional third party software such as Tecplot

3D view of results

System computer
The µPIV system includes a Data Acquisition and Control System, which ispreconfigured, tested and ready to use. All necessary hardware and software
installation is performed before delivery. The system computer is from aninternational manufacturer with worldwide presence.

System control with DynamicStudio– the most user-friendly and comprehensive software platform for scientificimagingPage 4Technical Specifications
Specifications

Specifications with standard objective

Magnification range

1.1x to 18x

Free working distance

20mm standard, others up to 135mm on request

Stereo full angle~62 degree (in air), ~46 degree (in water)/

2.2x to 36x/

3.6x to 57.6x

Online feedback in software System magnification, field of view, depth of field,
correlation depth, filter set, iris position

Order information
SeriesStereo microscope

Variant

Item noFluorescence Stereo Microscopewith motorized hand controller

9080M0601

Focused microstrobe illuminationand electronics for calibration

9080M0611

Calibration kit

Stereo µPIV calibration kit

9080M0621

3D microchannel

Microchannel for 3D flows(jet in cross flow & flow over step)

9080M0631

Adapter for converting stereo microscope
to 2D2C µPIV

9080M0641

1.1x to 18x

0,5x C-Mount Adapter for Stereo Microscope

9080M0651

2.2x to 36x

1,0x C-Mount Adapter for Stereo Microscope

9080M0661

3.6x to 57.6x

1,6x F-Mount Adapter for Stereo Microscope

9080M0671

LED illumination

2C-3C adapter

Solution Sheet 0454_v2 . Subject to change without notice. Copyright © 2019.
Dantec Dynamics. All Rights Reserved. www.dantecdynamics.com