2014 年 公開臨海実習
発展的海洋生物学・生態学コース
Advanced Course for Marine Biology and Ecology 2014

From August 7th to 12th, 2014
At Akkeshi Marine Station, Hokkaido University

1Advanced Course for Marine Biology and Ecology is an open marine course
co-organized by Akkeshi Marine Station and Muroran Marine Station of Field Science Center for
Northern Biosphere, Hokkaido University. It is aimed to provide the synthetic knowledge on marine
biology and ecology, including cell biology, developmental biology, physiological ecology, andecosystem ecology. We specially targeted macrophyte (seagrass and seaweed) community in cold
current region, which is the prominent character of the coastal seas of Hokkaido.

Venue
Akkeshi Marine Station, Hokkaido University
Address; Aikappu, Akkeshi, Hokkaido 088-1113, Japan
Tel: +81-153-52-2056, FAX: +81-153-52-2042
http://www.fsc.hokudai.ac.jp/akkeshi/AMS/en/index.html

Schedule
Aug. 7th (Thu) Guidance starts in the dining room at 18：00
Aug. 8th (Fri) 1st Day: Field collection and Thin-layer chromatography with algal pigments
Aug. 9th (Sat) 2nd Day: Cell biology of marine algae
Aug. 10th (Sun) 3rd Day: Field collection and seagrass productivity measurement
Aug. 11th (Mon) 4th Day: Food web structure in seagrass beds, Room cleaning, and Banquet
Aug. 12th (Tue) The course ends after breakfast.
>> The schedule can be change depending on weather conditions.
【Tidal chart in Akkeshi Bay】
LowestLowest tide

tide time(cm)

Aug. 8th

7:30

23.5

Aug. 9th

8:15

18.3

th

Aug. 10

8:55

15.6

Aug. 11th

9:31

15.7

Date

2

【Staff】
Masahiro Nakaoka 仲岡雅裕 (Akkeshi Marine Station, Hokkaido University, 北海道大学北方
生物圏フィールド科学センター厚岸臨海実験所・教授)
Taizo Motomura 本村泰三 (Muroran Marine Station, Hokkaido University, 北海道大学北方生
物圏フィールド科学センター室蘭臨海実験所・教授)
Chikako Nagazato 長里千香子 (Muroran Marine Station, Hokkaido University, 北海道大学北方生物圏フィールド科学センター室蘭臨海実験所・准教授)
Tomonori Isada 伊佐田智規 (Akkeshi Marine Station, Hokkaido University, 北海道大学北方
生物圏フィールド科学センター厚岸臨海実験所・助教)
Atsuko Tanaka 田中厚子 (Muroran Marine Station, Hokkaido University, 北海道大学北方生
物圏フィールド科学センター室蘭臨海実験所・特任助教)
Toshihiro Miyajima 宮島利宏 (Atmosphere and Ocean Research Institute, University of Tokyo東京大学大気海洋研究所・助教）
Yoshiyuki Tanaka 田中義幸 (JAMSTEC 海洋研究開発機構・研究員）【Students】
Name

Sex

University

Takayuki Kanki 神吉隆行

MOsaka University

B.A

Wan-Ting Su

FNational Taiwan University

M.S

Kullawit Limchularat

M

Kasetsart University

P.D

Muhamad Kemal Idris

M

Bogor Agricultural University

M.S

Allyn Duvin Pantallano

F

Mindanao State University

M.S

Tom Gerald Taer Genovia

M

Mindanao State University

M.S

Mikko Lacandazo Garcia

M

University of the Philippines

M.S【Assignments】
Each student has to submit a short report for each subject of the course. The deadline is August 29th.
Submission by email is recommended.
Subjects on 1st and 2nd day: to Prof. Motomura (motomura@fsc.hokudai.ac.jp)
Subjects on 3rd and 4th day: to Prof. Nakaoka (nakaoka@fsc.hokudai.ac.jp)

3

Course1st Day (Aug. 8th)

Biodiversity of algal community
OutlineMany species of seaweeds (macroalgae; green, red and brown algae) are found in the seashore of
Hokkaido, especially facing the Pacific. We can easily observed various colors, forms and sizes ofmacroalgae around Akkeshi Marine Station. On the first day, we will go to Daikoku‐jima island forcollecting the seaweeds. After coming back to the lab, we’ll make the dry specimens and sketching
the algal tissues (* Refer to the list of seaweeds in Akkeshi at the end of this textbook).
Additionally, we will start preparing the samples for tomorrow’s experiment (observation of
microtubules in brown algal cells).
Keywords: Brown algae, Field collection, Green algae, Macroalgae, Sketch, Specimens, Red algae
Schedule
07:00 – 07:30 Breakfast
08:30 –12:00 Field session #1: Trip to Daikoku‐jima Island by RV “Misago‐maru”. Observation ofalgal bed and collection of algae
12:30 – 13:30 Lunch
13:30 –17:30 Lab session #1 Identification of collected macroalgae and making specimens andsketches. Afterwards, preparation of tomorrow’s experiment will start.
18:00 – Dinner4

2nd Day (Aug. 9th)
[Experiment 1]

Cell biology of marine algae
OutlineBrown algae are prominent in cold region, and large kelps make the marine forest in the seashore.
They are included in heterokont algae with diatoms and several other phytoplankton. Heterokont
algae, of which phylogenetic position is far different from red and green algae (land plants), havecharacteristically evolved and adapted in sea. As the results, cellular features are unique, forexample, mitosis, cytokinesis, chloroplast and cytoskeleton. In this experiments, we will observe thecytoskeleton (microtubules and actin filaments) in the brown alga Dictyota dichotoma cells by
immunofluorescence microscopy and compare to those of animal and land plant cells.
Depending on the mature condition of the filed kelp Saccharina japonica var. diabolica, it may bepossible to try detecting the flagella of zoospores in the unilocular sporangium with special
antibodies.
Keywords: Antibodies, Actin, Brown algae, Chloroplasts, DAPI, Flagella, Immunofluorescencemicroscopy, Microtubules, Mitochondria, Nucleus, Nucleoid
Schedule
07:00 – 07:30 Breakfast
08:30 – 12:00 Start Experiment 1: Cell biology of marine algae
12:30 – 13:30 Lunch
13:30 – 17:00 Continuation of the Experiment 1 and initiation of Experiment 2: Algal pigments foraquatic photosynthesis.
18:00 – Dinner
Observation of microtubules (MT) of brown algae by indirect immunofluorescence microscopy
Materials: Cultural material, Dictyota dichotoma (AMIJIGUSA)Natural sample, Saccharina japonica var. diabolica (ONIKONBU)<Equipment>
Fluorescence microscope (Olympus epifluorescence microscope BX50WI‐FLA) with a CCD
camera (AxioCam from Zeiss)<Chemicals>∙ Poly‐L‐Lysine coated cover glass∙ Fixative (paraformaldehyde, glutaraldehyde)∙ PHEM buffer

5∙ PBS∙ blocking solution∙ DAPI (4', 6‐diamidino‐2‐phenylindole)∙ mounting medium∙ Primary antibodies
Dictyota dichotoma: Monoclonal anti‐actin antibody
Monoclonal anti‐b tubulin antibody
Saccharina japonica: Monoclonal anti‐b tubulin antibody
Polyclonal anti‐helmchrome antibory
Polyclonal anti‐mastigoneme antibody
・Secondary antibodies

Polyclonal anti‐mause IgG antibody
Polyclonal anti‐rabbit IgG antibody

PHEM buffer

PBS (1L)

Blocking solution

PIPES

60mM

NaCl

8g

Skim Milk

HEPES

25mM

KCl

0.2g

Normal goat

EGTA

10mM

Na2HPO4

0.7g

serum

5%

MgCl2

2mM

KH2PO4

0.2g

NaN3

0.1%

pH7.4

pH7.4

2.5%

in PBS<Procedure>I) Fixation<Chemical fixation>∙ Fix samples for 30 min in 3% paraformaldehyde, 0.1% glutaraldehyde, 2% NaCl in PHEM
buffer, and wash with PBS three times .
<Freeze‐substitution fixation>∙ Rapid fixation of samples in liquid propane (‐180 )∙ Keep samples in methanol for two days or more in a deep freezer (‐80 )∙ After keeping samples for 2 hr at ‐20 and 2hr at 4 , finally wash them with PBS threetimes at room temperature.
II) Softly squash samples between poly‐L‐lysine coated cover slip and slide glass. Cell are attachedon the former.
III) 5% Triton X‐100 in PBS for 30 min at room temperature and washing with PBS three times.
Purpose of this step is that;∙ elution of chlorophylls in chloroplasts, which possess strong red fluorescence under6

fluorescence microscope.
∙ easy penetration of antibodies (mw over 150K) into cellsIV) 0.1% NaBH4 (sodium borohydrate) in PBS for 20 min and washing with PBS three times.
∙ for omitting autofluorescence of glutaraldehyde, therefore it is not necessary in the caseof cryofixation.
V) Blocking solution for 30 min at room temperature.
∙ for preventing unspecific binding of antibodies.
VI) Primary antibody, (monoclonal anti‐actin and anti‐ tubulin antibody Goat) 90 min at room
temperature (or overnight) and washing with PBS three times.
VII) Secondary antibody, (polyclonal FITC (green fluorescence) or Rhodamine (red fluorescence)labeled anti‐mouse IgG (or anti‐rabbit IgG) antibody Goat) 60min at room temperature, andwashing with PBS three times.
VIII) DAPI (0.5 mg/mL) in PBS 10 min at room temp.
∙ DAPI (4', 6‐diamidino‐2‐phenylindole) binds to A‐T bond in DNA double helix, andpossesses strong blue fluorescence.
∙ Nucleus and nucluoids in mitochondria and chloroplasts can be observed.
IX) Mount samples with mounting medium containing glycerin and 0.2% p‐phenylenediamin forpreventing fading of fluorescence.

Microtubules (green) and nucleus (blue) in apical part of Dictyota dichotoma

7[Experiment 2]

Algal pigments for aquatic photosynthesis
OutlineMacro algae can be classified to three major groups; Green, Red and Brown algae. Here, we aregoing to learn about basic classification of macro algae. Then, we will address to the primarytaxonomic character, “colors of algae”. The reason of these colorful variations is because ofvarious photosynthetic pigments and combinations. Seawater is much more efficient absorberof ‘Light’ than air, and water depth is one of the biggest factors to change the light conditionsin the aquatic environment. For adaptation to such variable light condition, algae create manykinds of pigments and combinations of pigments. In this session, we are going to understandhow much various on algal photosynthetic pigments and trying to think why they are needed.
Keywords: Taxonomy, Green algae, Red algae, Brown algae, Photosynthesis, Pigments, Light
condition, Water depth, Chlorophyll, Thin‐layer chromatography

Thin‐layer chromatography of algal photosynthetic pigments<Materials>

Green, Red and Brown algae obtained in the Daikoku‐jima island<Chemicals>∙ Diethylether∙ Silica gel (powder)∙ TLC plate<Equipment>∙ Mortar and pestle∙ Test tubes with caps
∙ Pasteur glass pipette<Procedure>I)

Preparation
0. Make a line on TLC plate with pencil carefully*Don’t touch and scratch the surface of TLC plate

II)

Extraction of Pigments
1. Cut the specimens to section 2‐3cm2
2. Add a small spoon of silica gel powder and algal sections to Mortar
3. Homogenize algal section to be powder
4. Transfer algal powder to eppendorf tube
5. Add 0.5mL of diethylether to the tube and mix (named pigment solution)

8

III)

Loading materials
6. Add 1‐1.5 mL of ethyl acetate, diethylether and xylene to the test tubes
individually and seal with caps
7. Take pigment solution with Pasteur pipette and make a spot on TLC plate
8. Dry the pigment spots for several minutes
9. Put the TLC plates into the test tubes including each organic solvent
10. Watch out for over‐loading
11. Make a mark for final loading tip immediatelyIV)

Calculation of Rf values
12. Take photos just after finishing loading to keep data
13. Measure the distance from starting point
14. Calculate ‘retardation factor (Rf)’ values for each material and solvent

Figure. Example for various pigments with thin‐layer chromatography

9

3rd Day (Sunday, August 10)
Seagrass productivity measurement

Outline: Environmental conditions, such as light intensity, nutrient, temperature, current velocity,
desiccation and grazing pressure, determine the species composition, morphology, biomass andproductivities of macrophytes. We can observe at least four seagrass species around the Akkeshi
Marine Station. Consider the relationship between seagrass species distribution and environmental
factors through field trip on 3rd and 4th days. To measure the seagrass growth, hole-punch is one of
the major method. However, it is destructive and usually takes more than 1 week and considerable
effort. In this session, we will learn how to measure seagrasss productivity using PAM fluorometerin non-destructive manner, and discuss how environmental conditions affect the seagrass
productivity and distribution.

Keywords: Species composition, morphology, growth, fluorescence, Pulse Amplitude Modulation,
Rapid Light Curve, desiccation, emergence, light adaptation.

Schedule
7:00 – 7:30 Breakfast
8:30 – 12:00 Field session #2: Trip to a seagrass bed in Akkeshi Bay. Instruction of fieldmeasurement of photosynthetic activity of seagrass by Diving-PAM. Collection of seagrasses
for lab analyses”.
12:30 – 13:30 Lunch
13:30 – 17:30 Lab session #2: Observation of morphological characters of seagrasses. Measurementof photosynthetic activity of seagrasses under different stresses.
18:00 – Dinner

PAM fluorometer

Example of the RLC

10

4th Day (Monday, August 11)
Food web structure in seagrass beds

Outline: Submerged aquatic vegetation, such as salt marsh, seagrass bed and kelp forest, plays
important roles in coastal ecosystems because of their high productivity and biodiversity. However,they are susceptible to human-induced stresses such as eutrophication and disturbance affected byterrestrial and oceanic environmental changes. In this session, we will investigate the biodiversity
and food web structure of seagrass bed in Akkeshi by semi-quantitative measurements of species
diversity and abundance, and by gut-content analyses of major consumers. We will also learn aboutstable isotope technique for food web analyses. Based on these subjects, we will discuss what factors
affect biodiversity and ecosystem functions of coastal ecosystems.

Keywords: Field monitoring of biodiversity and biomass, Analyses of food web structure,
Measurements of coastal environments, Gut content analyses, Stable isotope analyses

Schedule
7:00 – 7:30 Breakfast
8:30 – 12:00 Field session #3: Trip to a Akkeshi-ko and Akkeshi Bay. Collection of seagrasses,
seagrass-associated organisms for community structure analyses and food web studies.
12:30 – 13:30 Lunch
13:30 – 16:30 Lab Session #3: Community ecology and stable isotope analyses of seagrass bed
assemblage.
16:30 – 17:30 Cleaning of laboratory and bedrooms
18:00 – Dinner (Banquet)11

List of seaweeds in Akkeshi and Muroran
室蘭・厚岸付近の海藻 （2012 年版）

Chlorophyta (Green Algae)

緑色植物門（A:Akkeshi, M:Muroran）

Ulotrichales ひびみどろ目
Collinsiellaceae らんそうもどき科
Collinsiella tuberculata Setchell et Gardner らんそうもどきM
Ulotrichaceae ひびみどろ科
Ulothrix flacca (Dillwyn) Thuret ひびみどろAMUlvales あおさ目
Capsosiphonaceae かぷさあおのり科
Capsosiphon groenlandicus (J. Agardh) Vinogradova ひもひとえぐさ A
Kornmanniaceae もつきひとえぐさ科
Kornmannia leptoderma (Kjellman) Bliding もつきひとえAM
Monostromataceae ひとえぐさ科
Monostroma angicava Kjellman えぞひとえぐさAM
Monostroma crassidermum Tokida あつかわひとえ
AProtomonostroma undulatum (Wittrock) Vinogradova しわひとえぐさ AM
Prasiolaceae かわのり科
Prasiola delicata Setchell et Gardner ひめいそかわのりAUlvaceae あおさ科
Blidingia minima (Kützing) Kylin ひめあおのり
AM
Ulva arasakii Chihara ながあおさ
M
Ulva compressa Linnaeus ひらあおのり
M
Ulva intestinalis Linnaeus ぼうあおのり
M
Ulva lactuca Linnaeus おおばあおさ
M
Ulva linza Linnaeus うすばあおのり
AMUlva pertusa Kjellman あなあおさ
AM
Ulva prolifera O.F. Müller すじあおのりAUlvaria fusca Ruprecht くろひとえぐさAMAcrosiphoniales もつれぐさ目
Acrosiphoniaceae もつれぐさ科
Spongomorpha duriuscula (Ruprecht) Collins もつれぐさ
ASpongomorpha heterocladia Sakai いぶりもつれぐさ
AM
Spongomorpha mertensii Setchell et Gardner かぎもつれぐさ
ASpongomorpha saxatilis (Ruprecht) Collins とげなしもつれぐさ
AM
Spongomorpha spiralis Sakai うずもつれぐさ
AM
Urospora penicilliformis (Roth) Areschoug しりおみどろAMCladophorales しおぐさ目
Cladophoraceae しおぐさ科
Chaetomorpha aerea (Dillwyn) Kützing たるがたじゅずもA
Chaetomorpha moniligera Kjellman たまじゅずもAM
Cladophora glemerata (Linnaeus) Kützing かもじしおぐさ
ACladophora opaca Sakai つやなししおぐさAMCladophora rudolphiana (C. Agardh) Kützing たまりしおぐさ
MCladophora sakaii Abbott あさみどりしおぐさ
MCladophora speciosa Sakai みやびしおぐさMCladophora stimpsonii Harvey きぬしおぐさ
AMRhizoclonium tortuosum (Dillwyn) Kuetzing ながもつれAM
Codiales みる目
Codiaceae みる科

12

Codium yezoense (Tokida) Vinogradova えぞみる（いもせみる）
Codium fragile (Suringar) Hariot みる
Bryopsidales はねも目
Bryopsidaceae はねも科
Bryopsis plumosa (Hudson) C. Agardh はねもDerbesiaceae つゆのいと科
Derbesia marina (Lyngbye) Solier ほそつゆのいと

A
M

M
A

Phaeophyceae (Brown Algae) 褐藻綱
Ectocarpales しおみどろ目
Ectocarpaceae しおみどろ科
Acinetospora crinita (Charmichael ex Harvey) Kornmann
M
Ectocarpus arctus Kützing けなししおみどろM
Ectocarpus fusiformis Nagai
A
Ectocarpus siliculosus (Dillwyn) Lyngbye しおみどろA
Ectocarpus yezoensis Yamada et Tanaka えぞしおみどろALaminarionema elsbetiae Kawai et Tokuyama らみなりおねまMSpongonema tomentosum (Hudson) Kützing かぎしおみどろAMStreblonema evagatum Setchell et Gardner こぶやどりみどろAPylaiellaceae ピラエラ科
Pylaiella littoralis (Linnaeus) Kjellman ぴらえら
AM
Sorocarpaceae ソロカルパ科
Botrytella micromora Bory いそぶどうAM
Botrytella reinboldii (Reinke) Kornmann & Sahling きたしおみどろ M
Ralfsiales いそがわら目
Ralfsiaceae いそがわら科
Analipus filiformis (Ruprecht) Papenfuss いとまつもM
Analipus gungii (Yendo) Kogame et Yoshida ぐんじまつもM
Analipus japonicus (Harvey) Wynne まつもAM
Ralfsia fungiformis (Gunnerus) Setchell et Gardner いそがわらARalfsia verrucosa (Areschoug) Areschoug いそいわたけ
AMChordariales ながまつも目
Acrotrichaceae にせもずく科
Acrothrix gracilis Kylin きたにせもずく
A
Acrothrix pacifica Okamura et Yamada にせもずく
M
Chordariaceae ながまつも科
Chordaria chordaeformis (Kjellman) Kawai et S. H. Kim ひもながまつも A
Chordaria flagelliformis (O.F. Müller) C. Agardh ながまつもAM
Eudesme virescens (Carmichael) J. Agardh にせふともずく A M
Heterosaundersella hattoriana Tokida からふともずく
A
Papenfussiella kuromo (Yendo) Inagaki くろもA
Saundersella simplex (Saunders) Kylin もつきちゃそうめんA
Sphaerotrichia divaricata (C. Agardh) Kylin いしもずく
AM
Elachistaceae なみまくら科
Elachista fusicola (Velley) AreschougA
Elachista tenuis Yamada ほそなみまくら
M
Halothrix ambigua Yamada そめわけぐさ
A M
Leathesiaceae ねばりも科
Leathesia difformis (Linnaeus) Areschoug ねばりもA M
Leathesia difformoides Takamatsu
M
Leathesia monilicellulata Takamatsu なんきんねばりもM
Phaeophysema sphaerocephala (Yamada) Tanaka, Uwai et Kawai
ひめねばりもA M
Myrionemataceae みりおねま科
Compronema secundum Setchell et GardnerA

13

Hecatonema terminale (Kützing) Kylin
M
Myrionema corunnae Sauvageau
M
Scytosiphonales かやものり目
Scytosiphonaceae かやものり科
Colpomenia bullosa (Saunders) Yamada わたもAM
Colpomenia peregrina (Sauvageau) Hamel うすかわふくろのり
AMMelanosiphon intestinalis (Saunders) Wynne きたいわひげ
AM
Petalonia fascia (O. F. Müller) Kuntze せいようはばのり
AMPetalonia zosterifolia (Reinke) Kuntze ほそばせいようはばのり
AMScytosiphon lomentaria (Lyngbye) Link かやものり
AM
Scytosiphon canaliculatus (Setchell et Gardner) Kogame かやもどき M
Scytosiphon tenellus Kogame ひらかやもM
Dictyosiphonales ういきょうも目
Coilodesmaceae えぞぶくろ科
Coilodesme cystoseirae (Ruprecht) Setchell et Gardner ほそえぞふくろ A
Coilodesme japonica Yamada えぞふくろ
AMDelamareaceae にせかやも科
Delamarea attenuata (Kjellman) Rosenvinge にせかやもA
Dictyosiphonaceae ういきょうも科
Dictyosiphon foeniculaceus (Hudson) Greville ういきょうもAM
Punctariaceae はばもどき科
Litosiphon groenlandicus var. japonicus Kawai et Kurogi いそひげも M
Pogotrichum yezoense (Yamada et Nakamura) Sakai et Saga こぶのひげ A M
Punctaria flaccida Nagai ちしまはばもどき
AM
Punctaria latifolia Greville はばもどき
M
Punctaria plantaginea (Roth) Greville はばだましAMSphacelariales くろがしら目
Sphacelariaceae くろがしら科
Sphacelaria plumigera Holmes はねくろがしらA
Sphacelaria rigidula Kützing みつでくろがしら
AM
Desmarestiales うるしぐさ目
Desmarestiaceae うるしぐさ科
Desmarestia ligulata (Stackhouse) Lamouroux うるしぐさ
AM
Desmarestia viridis (Müllar) Lamouroux けうるしぐさAM
Laminariales こんぶ目
Akkesiphycaceae こんぶもどき科
Akkesiphycus lubricum Yamada et Tanaka こんぶもどきAAlariaceae ちがいそ科
Alaria angusta Kjellman ほそばわかめA
Alaria crassifolia Kjellman ちがいそM
Alaria praelonga Kjellman あいぬわかめ
A
Undaria pinnatifida (Harvey) Suringar わかめ
M
Chordaceae つるも科
Chorda asiatica Sasaki et Kawai つるもAMCostariaceae すじめ科
Agarum clathratum Dumortier あなめ
AMCostaria costata (C. Agardh) Saunders すじめAMLaminariaceae こんぶ科
Arthrothamnus bifidus (Gmelin) Ruprecht ねこあしこんぶA
Saccharina sculpera Lane, Mayes, Druehl et Saunders がごめこんぶ M
Saccharina gyrata (Kjellman) Lane, Mayes, Druehl et Saunders とろろこんぶ A
Saccharina angustata (Kjellman) Lane, Mayes, Druehl et Saunder
みついしこんぶM
Saccharina coriacea (Miyabe) Lane, Mayes, Druehl et Saunders
がっがらこんぶ、あつばこんぶA14

Saccharina diabolica (Miyabe) Lane, Mayes, Druehl et Saunders おにこんぶ
Saccharina japonica (Areschoug) Lane, Mayes, Druehl et Saunders まこんぶ
Saccharina longissima (Miyabe) Lane, Mayes, Druehl et Saunders ながこんぶ
Saccharina longipedalis (Okamura) Lane, Mayes, Druehl et Saunders
えながこんぶ、かきじまこんぶ
Saccharina yendoana (Miyabe) Lane, Mayes, Druehl et Saunders
えんどうこんぶ
Laminaria yezoensis Miyabe ごへいこんぶ
Pseudochordaceae にせつるも科
Pseudochorda nagaii (Tokida) Inagaki にせつるも
Dictyotales あみじぐさ目
Dictyotaceae あみじぐさ科
Dictyopteris divaricata (Okamura) Okamura えぞやはず
Dictyota dichotoma (Hudson) Lamouroux あみじぐさ
Fucales ひばまた目
Fucaceae ひばまた科
Fucus distichus Linnaeus ssp. evanescens (C. Agardh) Powell ひばまた
Silvetia babingtonii (Harvey) E. Serrão, T.O. Cho, S.M. Boo et Brawley
えぞいしげ
Cystoseiraceae うがものく科
Coccophora langsdorfii (Turner) Greville すぎもく
Cystoseira crassipes (Turner) C. Agardh ねぶともく
Cystoseira hakodatensis (Yendo) Fensholt うがのもく
Sargassaceae ほんだわら科
Sargassum boreale Yoshida et Horiguchi ほっかいもく
Sargassum confusum C. Agardh ふしすじもく
Sargassum miyabei Yendo みやべもく
Sargassum muticum (Yendo) Fensholt たまははきもく
Sargassum thunbergii (Roth) Kuntze うみとらのおRhodophyta (Red Algae) 紅色植物門
Porphyridiales ちのりも目
Goniotrichaceae べにみどろ科
Stylonema alsidii (Zanardini) Drew べにみどろ
Erythropeltidales エリスロペルティス目
Erythropeltidaceae エリスロペルティス科
Erythrotrichia carnea (Dillwyn) J. Agardh ほしのいと
Bangiales うしけのり目
Bangiaceae うしけのり科
Bangia atropurpurea (Roth) C. Agardh うしけのり
Porphyra amplissima (Kjellman) Setchell et Hus べにたさPorphyta takadae Miura そめわけあまのり
Porphyta kuniedae Kurogi まるばあさくさのり
Porphyra kurogii Lindstrom ちしまくろのり
Porphyra moriensis Ohmi かやべのり
Porphyra occidentalis Setchell et Hus きいろたさPorphyra onoi Ueda おおののり
Porphyra pseudocrassa Yamada et Mikami まくれあまのり
Porphyra pseudolinearis Ueda うっぷるいのり
Porphyra seriata Kjellman いちまつのり
Porphyra suborbiculara Kjellman まるばあまのり
Porphyra variegata (Kjellman) Kjellman ふいりたさPorphyra yezoensis Ueda すさびのり

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Acrochaetiales アクロカエティウム目

15Acrochaetiaceae アクロケチウム科
Acrochaetium alariae (Jonsson) Bornet
Audouinella codii (Crouan) Garbary
Audouinella daviesii (Dillwyn) Woelkerling
Audouinella rhizoidea (Drew) Garbary
Rhodochorton purpureum (Lightfoot) Rosenvinge
Rhodochorton subimmersum Setchell et Gardner
Nemalionales うみぞうめん目
Liagoraceae こなはだ科
Helminthocladia australis Harvey べにもずく
Helminthocladia yendoana Narita ほそべにもずく
Nemaliaceae うみぞうめん科
Nemalion vermiculare Suringar うみぞうめん
Bonnemaisoniales かぎけのり目
Bonnemaisoniaceae かぎけのり科
Bonnemaisonia hamifera Hariot かぎのり
Gelidiales てんぐさ目
Gelidiaceae てんぐさ科
Gelidium subfastigiatum Okamura なんぶぐさ
Gelidium vagum Okamura よれくさ
Pterocladiella capillacea (Gmelin) Santelices et Hommersand おばくさ
Corallinales さんごも目
Corallinaceae さんごも科
Bossiella cretacea (Postels et Ruprecht) Johansen いそきり
Clathromophum compactum (Kjellman) Foslie あなあききたいしもCorallina pilulifera Postels et Ruprecht ぴりひば
Pneophyllum zostericola (Foslie) Fujita もかさ
Hapalidiaceae ハパリデウム科
Lithothamnion japonicum Foslie みやべおこし
Lithothamnion obtectulum (Foslie) Foslie あっけしおこし
Lithothamnion pacificum (Foslie) Foslie あっけしいぼいしMelobesia pacifica Masaki あばたもかさ
Clathromorphum reclinatum (Foslie) Adey かさきのこいしもPhymatolithon lenormandii (Areschoug) Adey あっけしいしも
Hildenbrandiales べにまだら目
Hildenbrandiaceae べにまだら科
Hildenbrandia rubra (Sommerfelt) Meneghini べにまだら
Gigartinales すぎのり目
Caulacanthaceae いそもっか科
Caulacanthus usutulatus (Turner) Kuetzing いそだんつう
Dumontiaceae りゅうもんそう科
Constantinea rosa-marina (Gmelin) Postels et Ruprecht おきつばら
Constantinea subulifera Setchell おおばおきつばら
Dumontia alaskana Tai, Lindstrom et Saunders りゅうもんそう
Dumontia simplex Cotton へらりゅうもん
Masudaphycus irregularis (Yamada) Lindstrom にせかれきぐさ
Hyalosiphonia caespitosa Okamura いそうめもどき
Neodilsea longissima (Masuda) Lindstrom ながあかば
Neodilsea tenuipes Yamada et Mikami まるばあかば
Neodilsea yendoana Tokida あかば
Endocladiaceae ふのり科
Gloiopeltis furcata (Postels et Ruprecht) J. Agardh ふくろふのり
Gigartinaceae すぎのり科
Chondrus armatus (Harvey) Okamura とげつのまた
Chondrus pinnulatus (Harvey) Okamura ひらことじ

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Chondrus yendoi Yamada et Mikami くろはぎんなんそう
Mazzaella japonica (Mikami) Hommersand あかばぎんなんそう
Gloiosiphoniaceae いとふのり科
Gloiosiphonia capillaris (Hudson) Carmichael いとふのり
Halymeniaceae むかでのり科
Grateloupia asiatica Kawaguchi et Wang むかでのり
Grateloupia divaricata Okamura かたのり
Grateloupia livida (Harvey) Yamada ひらむかで
Grateloupia turuturu Yamada つるつる
Grateloupia elliptica Holmes たんばのり
Polyopes affinis (Harvey) Kawaguchi et Wang まつのり
Kallymeniaceae つかさのり科
Callophyllis rhynchocarpa Ruprecht ひめとさかもどき
Cirrulicarpus gmelinii (Grunow) Tokida et Masaki えぞとさか
Euthora cristata (Turner) J. Agardh ゆうそら
Kallymenia ornata (Postels et Ruprecht) J. Agardh きたつかさのり
Kallymenia reniformis var. cuneata J. Agardh えぞつかさのり
Petrocelidaceae いぼのり科
Mastocarpus pacificus (Kjellman) Perestenko いぼのり
Phyllophoraceae おきつのり科
Ahnfeltiopsis flabelliformis (Harvey) Masuda おきつのり
Rhodophyllidaceae あみはだ科
Rhodophyllis capillaris Tokida いとあみはだ
Schizymeniaceae べにすなご科
Schizymenia dubyi (Chauvin) J. Agardh べにすなご
Solieriaceae みりん科
Turnerella mertensiana (Postels et Ruprecht) Schmitz えぞなめし
Tichocarpaceae かれきぐさ科
Tichocarpus crinitus (Gmelin) Ruprecht かれきぐさ
Gracilariales おごのり目
Gracilariaceae おごのり科
Gracilaria chorda Holmes つるしらもGracilaria vermiculophylla (Ohmi) Papenfuss おごもどき
Gracilaria asiatica Zhang et Xia おごのり
Ahnfeltiales いたにぐさ目
Ahnfeltiaceae いたにぐさ科
Ahnfeltia fastigiata (Endlicher) Makijenko ねつきいたにぐさ
Rhodymeniales まさごしばり目
Champiaceae わつなぎそう科
Champia parvula (C. Agardh) Harvey わつなぎそう
Lomentariaceae ふしつなぎ科
Lomentaria hakodatensis Yendo こすじふしつなぎ
Rhodymeniaceae まさごしばり科
Chrysymenia wrightii (Harvey) Yamada たおやぎそう
Sparlingia pertusa (Postels et Ruprecht) Saunders et al.あなだるす
Palmariales だるす目
Palmariaceae だるす科
Halosaccion firmum (Postels et Ruprecht) Kuetzing かたべにふくろのり
Halosaccion yendoi Lee べにふくろのり
Palmaria marginicrassa Lee あつばだるす
Palmaria palmata (Linnaeus) Kuntze だるす
Rhodophysemataceae ふちとりべに科
Pseudorhododiscus nipponicus Masuda べにごろも
Rhodophysema elegans (Crouan) Dixon うすふちとりべに
Rhodophysema georgii Batters ふちとりべに

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Rhodophysema odonthaliae Masuda et Ohta ひめふちとりべに
Ceramiales いぎす目
Ceramiaceae いぎす科
Antithamnion densum (Suhr) Howe きぬいとふたつがさね
Antithamnion nipponicum Yamada et Inagaki ふたつがさね
MAntithamnionella spirographidis (Schiffner) Wollaston ほそがさね
Aglaothamnion callophyllidicola (Yamada) Boo, Lee, Rueness et Yoshida
きぬいとぐさCampylaephora crassa (Okamura) Nakamura ふといぎす
Campylaephora hypnaeoides J. Agardh えごのり
Ceramium cimbricum Petersen まつばらいぎす
Ceramium fastigiramosum Boo et Lee ひめいぎす
Ceramium japonicum Okamura はねいぎす
Ceramium kondoi Yendo いぎす
Herpochondria elegans (Okamura) Itono さえだ
Neoptilota asplenioides (Esper) Kylin かたわべにひば
Pterothamnion yezoense (Inagaki) Athanasiadis et Kraft よつがさね
Ptilota filicina J. Agardh くしべにひば
Plilota phacelocarpoides Zinova こばのくしべにひば
Scagelia pylaiesii (Montagne) Wynne からふとよつがさね
Tokidaea corticata (Tokida) Yoshida べにはねもDelesseriaceae このはのり科
Acrosorium yendoi Yamada はいうすばのり
Acrosorium polyneurum Okamura すじうすばのり
Branchioglossum nanum Inagaki ひめむらさき
Congregatocarpus pacificus (Yamada) Mikami このはのりDelesseria serrulata Harvey ぬめはのり
Erythroglossum pinnatum Okamura ひめうすべに
Neoholmesia japonica (Okamura) Mikami すずしろのり
Neohypophyllum middendorfii (Ruprecht) Wynne ながこのはのり
Hideophyllum yezoense (Yamada et Tokida) Zinova あつばすじぎぬ
Phycodrys riggii Gardner かしわばこのはのり
Phycodrys radicosa (Okamura) Yamada et Inagaki ひめこのはのり
Tokidadendron kurilensis (Ruprecht) Perestenko らいのすけこのはDasyaceae だじあ科
Dasya sessilis Yamada えなしだじあ
Heterosiphonia japonica Yendo いそはぎ
Heterosiphonia pulchra (Okamura) Falkenberg しまだじあ
Rhodomelaceae ふじまつも科
Benzaitenia yenoshimensis Yendo べんてんもChondria crassicaulis Harvey ゆな
Chondria dasyphylla (Woodward) C. Agardh やなぎのり
Chondria tenuissima (Withering) C. Agardh ほそやなぎのり
Enelittosiphonia stimpsonii (Harvey) Kudo et Masuda まきいとぐさ
Janczewskia morimotoi Tokida もりもとそぞまくら
Laurencia nipponica Yamada うらそぞAM
Neorhodomela aculeata (Perestenko) Masuda ふじまつも
Neorhodomela munita (Perestenko) Masuda いとふじまつ
Neorhodomela oregona (Doty) Masuda あっけしふじまつも
Neosiphonia harveyi (Bailey) Kim et al. きぶりいとぐさ
Neosiphonia yendoi (Segi) Kim et Lee えんどういとぐさ
Odonthalia annae Perestenko ありゅうしゃんのこぎりひば
Odonthalia corymbifera (Gmelin) Greville はけさきのこぎりひば
Odonthalia macrocarpa Masuda おおのこぎりひば
Odonthalia yamadae Masuda あっけしのこぎりひば

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Polysiphonia fragilis Suringar くろいとぐさ
Polysiphonia morrowii Harvey もろいとぐさ
Pterosiphonia arctica (J. Agardh) Setchell et Gardner いなぼぐさ
Pterosiphonia bipinnata (Postels et Ruprecht) Falkenberg いとやなぎ
Rhodomela lycopodioides f. tenuissima (Ruprecht) Kjellman
みやびふじまつもRhodomela sachalinensis Masuda からふとふじまつもRhodomela teres (Perestenko) Masuda ほそばふじまつもSymphyocladia latiuscula (Harvey) Yamada いそむらさき
Symphyocladia marchantioides (Harvey) Falkenberg こざねも

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