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Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
Date of issue for the 1st edition : 28/Jan/2014
Date of revision : 07/Dec/2022

Safety Data Sheet

Section 1. Identification of the substance/mixture and of the company/undertaking
Product identifier:Product name: Chloroform
Reference number(SDS):28560jis_J_E1-4
Product type：
Reagent
Details of the supplier of the safety data sheet
Manufacturer/Supplier: JUNSEI CHEMICAL CO., LTD.
Address: 1-6, Ohmano-cho, Koshigaya-shi, Saitama 343-0844, Japan
Division: Quality Assurance Department
Telephone number: +81-48-986-6161
FAX: +81-48-989-2787
e-mail address: shiyaku-t@junsei.co.jp

Section 2. Hazards identification
GHS classification and label elements of the product
Classification of the substance or mixture
HEALTH HAZARDS
Acute toxicity (Oral): Category 4
Acute toxicity (Inhalation): Category 3
Skin corrosion/irritation: Category 2
Serious eye damage/eye irritation: Category 1
Germ cell mutagenicity: Category 2
Carcinogenicity: Category 2
Reproductive toxicity: Category 2
Specific target organ toxicity - single exposure: Category 1(respiratory system, cardiovascular system, liver,kidney)Specific target organ toxicity - single exposure: Category 3 (Narcosis)
Specific target organ toxicity - repeated exposure: Category 1(central nervous system, respiratory system,
liver, kidney)
ENVIRONMENT HAZARDS
Hazardous to the aquatic environment (Acute): Category 3
Hazardous to the aquatic environment (Long-term): Category 1(Note) GHS classification without description: Not classified/Classification not possible
Label elements

Signal word: Danger
HAZARD STATEMENT
H302-Harmful if swallowed
H331-Toxic if inhaled
H315-Causes skin irritation
H318-Causes serious eye damage
H341-Suspected of causing genetic defects
H351-Suspected of causing cancer

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Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
H361-Suspected of damaging fertility or the unborn child
H370-Causes damage to organs
H336-May cause drowsiness or dizzinessH372-Causes damage to organs through prolonged or repeated exposure
H402-Harmful to aquatic life
H410-Very toxic to aquatic life with long lasting effects
PRECAUTIONARY STATEMENT
Prevention
Obtain special instructions before use.
Do not handle until all safety precautions have been read and understood.
Avoid release to the environment.
Do not breathe vapors.
Use only outdoors or in a well-ventilated area.
Wash contaminated parts thoroughly after handling.
Wear protective gloves.
Wear eye protection/face protection.
Use personal protective equipment as required.
Do not eat, drink or smoke when using this product.
Response
Collect spillage.
Get medical advice/attention if you feel unwell.
IF exposed or concerned: Get medical advice/attention.
Immediately call a POISON CENTER/doctor/physician.
Call a POISON CENTER/doctor/physician if you feel unwell.
IF exposed or concerned: Call a POISON CENTER/doctor/physician.
IF INHALED: Remove person to fresh air and keep comfortable for breathing.
IF ON SKIN: Wash with plenty of soap and water.
If skin irritation occurs: Get medical advice/attention.
Take off contaminated clothing and wash it before reuse.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if
present and easy to do. Continue rinsing.
IF SWALLOWED: Rinse mouth. Call a POISON CENTER/doctor/physician if you feel unwell.
Storage
Store in a well-ventilated place. Keep container tightly closed.
Store locked up.
Disposal
Dispose of contents/container in accordance with local/national regulation.

Section 3. Composition/information on ingredients
Mixture/Substance selection:
Substance
Ingredient name:Chloroform
Content (%):99.0 <Chemical formula:CHCl3
Chemicals No, Japan:2-37
CAS No.:67-66-3
MW:119.38
ECNO:200-663-8
Stabilizing additives
Ethanol (0.3～1.0%)
Note : The figures shown above are not the specifications of the product.

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Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
Section 4. First-aid measures
Descriptions of first-aid measures
General measures
Get medical advice/attention if you feel unwell.
Immediately call a POISON CENTER/doctor/physician.
Keep victim warm and quiet.
Call emergency medical service.
Do not use mouth-to-mouth method if victim ingested or inhaled the substance; give
artificial respiration with the aid of a pocket mask equipped with a one-way valve or otherproper respiratory medical device.
Effects of exposure (inhalation, ingestion or skin contact) to substance may be delayed.
IF INHALED
Remove person to fresh air and keep comfortable for breathing.
Give artificial respiration if victim is not breathing.
Administer oxygen if breathing is difficult.
Call a POISON CENTER/doctor/physician if you feel unwell.
IF ON SKIN (or hair）
Take off immediately all contaminated clothing. Rinse skin with water or shower.
Wash with plenty of soap and water.
If skin irritation or rash occurs: Get medical advice/attention.
If skin irritation occurs: Get medical advice/attention.
Remove and isolate contaminated clothing and shoes.
For minor skin contact, avoid spreading material on unaffected skin.
IF IN EYES
Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy
to do. Continue rinsing.
If eye irritation persists: Get medical advice/attention.
IF SWALLOWED
Rinse mouth.
If victim is conscious, give 1 - 2 glasses of water.
Call a POISON CENTER/doctor/physician if you feel unwell.
Most important symptoms and effects, both acute and delayed(Symptoms when inhalation or ingestion)
Nausea. Abdominal pain. Headache. Drowsiness. Cough. Dizziness. Unconsciousness.. Vomiting.
(Symptoms when skin and/or eye contact)
Dry skin. Conjunctival redness of the eyes. Redness of the skin. Pain.
Indication of any immediate medical attention and special treatment needed
Ensure that medical personnel are aware of the material(s) involved, and take precautions
to protect themselves.

Section 5. Fire-fighting measures
Extinguishing media
Suitable extinguishing media
Use appropriate extinguishing media suitable for surrounding facilities.
The product is non-flammable.
Unsuitable extinguishing media
Do not use direct water jet.
Specific hazards arising from the substance or mixture
Containers may explode when heated.
Fire may produce irritating, corrosive and/or toxic gases.
Runoff from fire control or dilution water may cause pollution.

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Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
Advice for firefighters
Specific fire-fighting measures
Evacuate non-essential personnel to safe area.
Cool container with water spray.
Special protective equipment and precautions for fire-fighters
Wear fire resistant or flame retardant clothing.
Wear protective gloves/protective clothing/eye protection/face protection.
Firefighters should wear self-contained breathing apparatus with full face peace operated
positive pressure mode.

Section 6. Accidental release measures
Personnel precautions, protective equipment and emergency procedures
Keep unauthorized personnel away.
In case of contact with substance, immediately flush skin or eyes with running water for atleast 20 minutes.
Ventilate area until material pick up is complete.
Wear proper protective equipment.
Do not touch damaged containers or spilled material unless wearing appropriate protective
clothing.
EVACUATION : Spill: See the Table of Initial Isolation and Protective Action Distances forhighlighted substances. For non-highlighted substances, increase, in the downwind
direction, as necessary, the isolation distance shown under “PUBLIC SAFETY”.
Environmental precautions
Avoid release to headsprings, rivers, lakes, ocean and groundwater.
Methods and materials for containment and cleaning up
Absorb spill with inert material (dry sand, earth, et al), then place in a chemical waste container.
Preventive measures for secondary accident
Collect spillage.
Stop leak if you can do it without risk.
Prevent entry into waterways, sewers, basements or confined areas.
Do not get water inside containers.
Keep out of low areas.

Section 7. Handling and storage
Precautions for safe handling
Preventive measures(Exposure Control for handling personnel)
Do not breathe vapors.
(Protective measures against fire and explosion)
Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
(Exhaust/ventilator)
Exhaust/ventilator should be available.
(Safety treatments)
Avoid contact with skin.
Avoid contact with eyes.
Safety Measures
Obtain special instructions before use.
Do not handle until all safety precautions have been read and understood.
Use only outdoors or in a well-ventilated area.
Wear protective gloves/protective clothing/eye protection/face protection.
Use personal protective equipment as required.
When using do not eat, drink or smoke.

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Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
Any incompatibilities
Strong bases, Strong oxidizing agents, Aluminium, Magnesium, Zinc. should not be mixed withthe chemicals.
Advice on general occupational hygiene
Wash contaminated parts thoroughly after handling.
Do not eat, drink or smoke when using this product.
Take off contaminated clothing and wash it before reuse.
Storage
Conditions for safe storage
Store in a well-ventilated place. Keep container tightly closed.
Keep cool. Protect from sunlight.
Store in accordance with local/national regulation.
Store locked up.
Container and packaging materials for safe handling data is not available.
Specific end use(s)See information in Section 7.1 and 7.2 for handling and storage recommendations. See
Section 8 for exposure controls and personal protection recommendations.

Section 8. Exposure controls/personal protection
Control parameters
Control value
Japan control value (2009) <= 3ppm
Adopted value
JSOH(2005) 3ppm; 14.7mg/m3 (dermal)
ACGIH(1995) TWA: 10ppm (Liver & embryo/fetal dam; CNS impair)
Exposure controls
Appropriate engineering controls
Do not use in areas without adequate ventilation.
Eye wash station should be available.
Washing facilities should be available.
Individual protection measures
Respiratory protection
Wear respiratory protection.
Wear positive pressure self-contained breathing apparatus (SCBA).
Hand protection
Wear protective gloves. Recommended material(s): viton
Inspect before use and replace worn or damaged gloves.
Contact the glove manufacturer for specific advice on glove selection and breakthroughtimes for your use conditions.
Eye protection
Wear safety glasses with side-shields.
Wear eye/face protection.
Skin and body protection
Wear impervious clothing and boots in case of repeated or prolonged treatment.

Section 9. Physical and Chemical Properties
Information on basic physical and chemical properties
Physical state: Volatile liquid
Color: Colorless
Odor: Characteristic odor
Odor threshold: 85ppm; 250～1000mg/cm3
Melting point/Freezing point: -64℃

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Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
Boiling point or initial boiling point: 62℃
Boiling range data is not available.
Flammability (gases, liquids and solids): Non-flammable
Lower and upper explosion limit/flammability limit data is not available.
Flash point: Non-flammableAuto-ignition temperature data is not available.
Decomposition temperature data is not available.
Self-Accelerating Decomposition Temperature/SADT data is not available.
pH data is not available.
Dynamic viscosity: 0.56mPas(20℃)
Kinematic viscosity: 0.38mm2/s(20℃)
Solubility:
Solubility in water: 0.8g/100 ml (20℃)
Solubility in solvent: Miscible with ethanol (99.5) and diethyl ether.
n-Octanol/water partition coefficient: log Pow1.97
Vapor pressure: 21.2kPa(20℃)
Density and/or relative density: 1.48g/cm3(20℃)
Relative vapor density (Air=1): 4.12
Relative density of the Vapor/air - mixture at 20℃ (Air = 1): 1.7
Particle characteristics data is not available.
Other information
Critical temperature: 263.2 ℃
Evaporation rate data is not available.
VOC data is not available.

Section 10. Stability and Reactivity
Reactivity
Runaway polymerization will not occur.
Chemical stability
Stable under normal storage/handling conditions.
Possibility of hazardous reactions
The vapour is heavier than air.
Decomposes on contact with hot surfaces or flames. This produces toxic and corrosive fumesof of hydrogen chloride, phosgene and chlorine.
Reacts violently with strong bases, strong oxidants and some metals such as aluminium,
magnesium and zinc. This generates fire and explosion hazard.
Attacks plastics(eg. Polyvinyl chloride), rubber(eg. Natural rubber) and coatings.
Conditions to avoid
Contact with incompatible materials.
Open flames. Heating. Sparks. Light.
Incompatible materials
Strong bases, Strong oxidizing agents, Aluminium, Magnesium, Zinc.
Hazardous decomposition products
Carbon oxides, Hydrogen chloride, Phosgene, Chlorine.

Section 11. Toxicological Information
Information on toxicological effects
Acute toxicity
Acute toxicity (Oral)[Product]Category 4, Harmful if swallowed7/11
Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022[Data for components of the product][GHS Cat. Japan, base data]male rat LD50=445mg/kg (CLH Report, 2010)
Acute toxicity (Dermal)[Product]
Based on available data, the classification criteria are not met.
[Data for components of the product][GHS Cat. Japan, base data]
rabbit LD50 >3980mg/kg (AICIS IMAP, 2014)
Acute toxicity (Inhalation)[Product]Category 3, Toxic if inhaled[Data for components of the product][GHS Cat. Japan, base data]
vapor: rat LC50=9.2g/m3/6hr (cal.: 11.3g/m3/4hr, 2310ppm/4hr) (AICIS IMAP, 2014)
Labor standard law, Japan; Toxic
Chloroform
Irritant properties
Skin corrosion/irritation[Product]Category 2, Causes skin irritation[Data for components of the product][GHS Cat. Japan, base data]
rabbit : moderate skin necrosis et al (EHC 163, 1994)
Serious eye damage/irritation[Product]Category 1, Causes serious eye damage[Data for components of the product][GHS Cat. Japan, base data]
rabbit : severe irritation (EHC 163, 1994)
Sensitization
Respiratory sensitization[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Skin sensitization[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Germ cell mutagenicity[Product]Category 2, Suspected of causing genetic defects[Data for components of the product][GHS Cat. Japan, base data]
cat. 2; CERI/NITE Hazard Assessment Report, 2008
Carcinogenicity[Product]Category 2, Suspected of causing cancer[Data for components of the product][GHS Cat. Japan, base data]
cat.2; IARC Gr. 2B (IARC, 1999 et al.)

8/11
Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022[IARC]
Group 2B : Possibly carcinogenic to humans[ACGIH]
A3(1995) : Confirmed Animal Carcinogen with Unknown Relevance to Humans[JSOH]
Group 2B: The agents which are probably or possibly carcinogenic to humans[NTP]
RAHC : Reasonably Anticipated to be Human Carcinogens[EPA]
NL; Not likely to be carcinogenic to humans(Any route of exposure under exposure conditions
that do not cause cytotoxicity and cell regeneration)
L;Likely to be carcinogenic to humans(All routes of exposure under high-exposure conditions
that lead to cytotoxicity and regenerative hyperplasia in susceptible tissues)(1999)[EU]
Category 2; Substances suspected human carcinogens
Reproductive toxicity[Product]Category 2, Suspected of damaging fertility or the unborn child[Data for components of the product][GHS Cat. Japan, base data]
cat. 2; CERI/NITE Hazard Assessment Report, 2008
Specific target organ toxicity (STOT)
STOT-single exposure[Product]Category 1, Causes damage to organs
Category 3, May cause drowsiness or dizziness[Data for components of the product][cat.1][GHS Cat. Japan, base data]
cardiovascular system, liver, respiratory system, kidneys (EU-RAR, 2007 et al.)[cat.3 (drow./dizz.)][GHS Cat. Japan, base data]narcotic effect (EU-RAR, 2007 et al.)STOT-repeated exposure[Product]Category 1, Causes damage to organs through prolonged or repeated exposure[Data for components of the product][cat.1][GHS Cat. Japan, base data]
liver, central nervous system, respiratory system, kidneys (PATTY 6th, 2012; CERI/NITE Hazard
Assessment Report, 2008)
Aspiration hazard[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.

Section 12. Ecological Information
Toxicity
Aquatic toxicity[Product]Category 3, Harmful to aquatic life
Category 1, Very toxic to aquatic life with long lasting effects

9/11
Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022[Data for components of the product]
Hazardous to the aquatic environment (Acute)[GHS Cat. Japan, base data]
Algae (Chlamydomonas) EC50=13.3mg/L/72hr (EU-RAR, 2007)
Hazardous to the aquatic environment (Long-term)[GHS Cat. Japan, base data]
Fish (rainbow trout) NOEC＝0.059mg/L/21days(Environmental Risk Assessment for Chemicals by MOE in Japan vol. 2, 2003)
Water solubility[Data for components of the product]
0.8 g/100 ml (20℃) (ICSC, 2000)
Persistence and degradability[Data for components of the product]
Not rapidly degradable [BOD_Degradation: 0%/14 days; GC_Degradation: 4.6%/14 days(MITI official bulletin)]
Bioaccumulative potential[Data for components of the product]log Pow=1.97 (ICSC, 2000) ; BCF=13 (Registered chemicals data check & review, Japan)
Mobility in soil
Mobility in soil data is not available.
Other adverse effects
Ozone depleting chemical data is not available.

Section 13. Disposal considerations
Description of waste residues and information on their safe handling and methods of disposal,
including the disposal of any contaminated packaging
Waste treatment methods
Avoid release to the environment.
Dispose of contents/container in accordance with local/national regulation.

Section 14. Transport Information
UN No., UN CLASSUN No. or ID No.: 1888
UN Proper Shipping Name : CHLOROFORMClass or division (Transport hazard class) : 6.1
Packing group : III
ERG GUIDE No.: 151
IMDG Code (International Maritime Dangerous Goods Regulations)
UN No.: 1888
Proper Shipping Name : CHLOROFORMClass or division : 6.1
Packing group : III
IATA Dangerous Goods Regulations
UN No.: 1888
Proper Shipping Name : CHLOROFORMClass or division : 6.1
Hazard labels : Toxic
Packing group : III
Environmental hazards
MARPOL Annex III - Prevention of pollution by harmful substances
Marine pollutants (yes/no) : yes10/11Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
MARPOL Annex V - Prevention of pollution by garbage discharge
Specific target organ toxicity - repeated exposure: cat.1
Chloroform
Hazardous to the aquatic environment - long-term hazard: cat.1, 2
Chloroform
Maritime transport in bulk according to IMO instruments
Noxious Liquid ; Cat. Y
Chloroform(Y-146)

Section 15. Regulatory Information
Safety, health and environmental regulations/legislation specific for the substance or mixture
U.S. Toxic Substances Control Act (TSCA) Inventory
Chemicals listed in TSCA Inventory
67-66-3; 64-17-5
All components are listed or exempted.
Superfund Amendments and Reauthorizations Act (SARA), Title III
SARA 313 (TRI)
Chloroform
Other regulatory information
We are not able to check up the regulatory information with regard to the substances inyour country or region, therefore, we request this matter would be filled by your responsibility.
Regulatory information with regard to this substance in your country or in your region
should be examined by your own responsibility.
Ensure this material in compliance with federal requirements and ensure conformity to local regulations.
Regulatory information in this section are limited to intentional ingredient(s), but does
not contain information on non-intentional ingredients or impurities which are not informed by supplier(s).
Chemical safety assessment
Advice on safe handling for this product can be found in sections 7 and 8 of this SDS.

Section 16. Other information
GHS classification and labelling
H302-Acute toxicity, Category 4: H302 Harmful if swallowed
H331-Acute toxicity, Category 3: H331 Toxic if inhaled
H315-Skin corrosion/irritation, Category 2: H315 Causes skin irritation
H318-Serious eye damage/eye irritation, Category 1: H318 Causes serious eye damage
H341-Germ cell mutagenicity, Category 2: H341 Suspected of causing genetic defects
H351-Carcinogenicity, Category 2: H351 Suspected of causing cancer
H361-Reproductive toxicity, Category 2: H361 Suspected of damaging fertility or the unborn child
H370-STOT - single exposure, Category 1: H370 Causes damage to organs
H336-STOT - single exposure, Category 3, Respiratory tract irritation: H336 May cause
drowsiness or dizziness.
H372-STOT - Repeated exposure, Category 1: H372 Causes damage to organs through prolonged
or repeated exposure
H402-Hazardous to the aquatic environment, short-term (acute), Category 3: H402 Harmful toaquatic life
H410-Hazardous to the aquatic environment, long-term (chronic), Category 1: H410 Very toxic
to aquatic life with long lasting effects
References and sources for data
Globally Harmonized System of classification and labelling of chemicals, UN
Recommendations on the TRANSPORT OF DANGEROUS GOODS 21th edit., 2019 UN
IMDG Code, 2018 Edition (Incorporating Amendment 39-18)
IATA Dangerous Goods Regulations (62nd Edition) 2021

11/11Chloroform,JUNSEI CHEMICAL CO., LTD.,28560jis_J_E1-4,07/Dec/2022
2020 EMERGENCY RESPONSE GUIDEBOOK (US DOT)
2022 TLVs and BEIs. (ACGIH)
JIS Z 7252 : 2019
JIS Z 7253 : 20192021 Recommendation on TLVs (JSOH)
Supplier's data/information
Chemicals safety data management system "GHS Assistant" Version 4.19 (https://www.asahi-ghs.com/)
NITE Chemical Risk Information Platform "NITE-CHRIP"(https://www.nite.go.jp/en/chem/chrip/chrip_search/systemTop)
GHS Classification Guidance for Enterprises 2019 Revised Edition (Ver. 2.0) （Mar. 2020, METI)
Abbreviations and acronyms
SDS (Safety Data Sheet)
LD50 (Lethal Dose, 50％)
LC50 (Lethal Concentration, 50％)
IARC (International Agency for Research on Cancer)
ACGIH (American Conference of Governmental Industrial Hygienists)
EPA (US Environmental Protection Agency)
NTP (US National Toxicology Program)
JSOH (Japan Society for Occupational Health)EU (European Union)
EC50 (Effective Concentration, 50％)
NOEC (No Observed Effect Concentration)
BOD (Biochemical Oxygen Demand)
COD (Chemical Oxygen Demand)
BCF (Bioconcentration Factor)anh (anhydride)
General Disclaimer
This data sheet was created based on the information we currently have and may be revised
according to new information. In addition, the precautions apply only to normal handling,
and in the case of special handling, please make adequate countermeasure to maintain yoursafety.
The data given here is based on current knowledge and experience. The purpose of this
Safety Data Sheet is to describe the products in terms of their safety requirements. The
data does not signify any warranty with regard to the products' properties.
The GHS classification data given here is based on current Japan official data (NITE
published in 2021).