ᇹ ᵖ ‫ׅ‬ʴ᧓ऴ‫˟ܖإ‬ᴾ ἯἋἑὊႆᘙʖᆜᨼᴾ Ⴘഏᴾᴾᾀώ Ẑሂ၅іᙀΝೞನểẲềỉᢃѣႝឋᵋሂ᧓ỉ෇ѣᡲ᧙ỉ‫ࢍف‬ẑᴾཅ‫ޛ‬๾ɟẆѨദርẆദᧉဌʁẆங஭ࢧဏẆ᣺‫ܢ‬ଢΨẆཅ‫ئ‬๾ɟὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵏᴾᴾᾁώ ẐἰἻἏὅἻὅἜὊẦỤỉऴ‫إ‬ἍὅἉὅἂẑᴾ
ᕲဋᴾ थὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵓᴾᴾᾂώ Ẑᛐ‫ܭ‬ᢡˡỽỸὅἍἻὊỉἇỶỺὅἋἅἱἷἝἃὊἑὊểẲềỉࢫлẑᴾ‫߃ޢ‬औМẆႻຓࢄဃẆ๚ຓπ‫܇‬Ẇܴ˜ẨỚ‫܇‬Ẇ߷ႸᘽὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵕᴾᴾᾃώ ẐἅὅἦἘὅἉἯὊἚἧỻἼỼử෇ဇẲẺ‫ܖ‬፼ૅੲỆ᧙ẴỦᎋ‫ݑ‬ẑᴾૼႸჇኔẆᧈක‫ݩ‬ɟẆ‫ޛ‬ఌ̮ʚẆೌᕲ̢ࢠẆྚஙഗʍὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵗᴾᴾᾄώ Ẑᐮ࠿ỴἿἰἍἻἦὊỉ၊ၘСࣂỉᅕኺဃྸ‫ܖ‬ႎ౨᚛ẑᴾ‫׽ש‬Ӑ፦Ẇ༾ఘᅙᨻẆ߷Ҿ᩹ࢀẆ‫˱ܢ‬፦჆ὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵏᵏᴾ
ᴾᾅώ Ẑ‫ٶ‬ज़ᙾႎễ‫ٳ‬ᢿХນỉ‫҄٭‬Ệ‫ݣ‬ẴỦἤἚᏯ෇ѣỉ౨᚛ẑᴾ᩷‫ޛ‬ᴾ ૞Ẇం؉჆ʍὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵏᵐᴾᴾᾆώ Ẑிଐஜ‫ٻ‬ᩗ໎Ểᘮ໎ẲẺ‫܇‬ỄờẺẼỉܿජ‫׹‬ỿἵὅἩỆấẬỦܼଈểỉऴ‫إ‬ʩ੭ᴾᵋᛦ௹୿Ὁ‫إ‬ԓ୿ỉᢃဇửᡫẲềᵋẑᴾ
ႻຓࢄဃẆ‫߃ޢ‬औМẆ߷ႸᘽẆ๚ຓπ‫܇‬Ẇܴ˜ẨỚ‫܇‬ὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵏᵑᴾ
ᴾᾇώ Ẑͤࠝᎍử‫ݣ‬ᝋểẲẺἩἿἏἙỵᨦܹႆဃᛢ᬴ܱ᫆ểᏯඬᚘยẑᴾ
ʻʟዋ፦‫܇‬Ẇ༾ఘᅙᨻẆ᧙գ‫܇‬Ẇ߷Ӎ૓ဏὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵏᵓᴾᴾᾈώ ẐᾁỽἽἢ࿢ཞἭἋἧỳἓἊὅᣠή‫˳ࣱီܖ‬ỉဃྸ˺ဇỉ౨᚛ẑᴾ
᣼߃ዋ፦ẆࢸᕲჇ᣺Ẇ‫ؗ‬ဋ୑፦Ẇᑶຓ̲ԧẆౕ‫ݎ‬૓ẆᤠஙܑබẆᴾ‫ݱ‬௎ᡶẆܴ˜ẨỚ‫܇‬ὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵏᵕᴾᴾᾀ὿ώẐѣཋኬᏘỉ༏ἋἚἾἋऴ‫إ‬ˡᢋỆ᧙ɨẴỦૼᙹἅἾἋἘἼἽἂἽἅἉἛᵆᵡᵥᵇӳ঺ᣞእẑᴾ
ᅸ‫܇ځޛ‬Ẇ˱ẉங঺൶ẆສဋѠẆᑶຓ̲ԧẆࢸᕲჇ᣺Ẇᧈ‫ش‬᩹‫܇‬Ẇ‫ݱ‬௎ᡶẆᴾ
࠯௎፯ᨺẆܴ˜ẨỚ‫܇‬ὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉ ὉὉὉὉὉὉὉὉὉὉὉὉὉὉ ᵮᵐᵏᴾ
ᴾᾀᾀώẐɶ‫׎‬ỉͤࡍ૙Ꮛỉྵཞể૙Ꮛ૙஬ỉ᧏ႆӏỎऴ‫إ‬σஊẑᴾႬᴾ ᡏẆ๚ຓπ‫܇‬Ẇܴ˜ẨỚ‫܇‬ὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉὉᵮᵐᵑᴾ

╭∋ഭ⵬ఘᯏ᭴ߣߒߡߩ
ㆇേ⊹⾰г╭㑆ߩᵴേㅪ㑐ߩჇᒝ‐ጊẢ৻ 1,2, ൎᱜ▸ 2, ᱜ㐷↱ਭ 3, ᧁ᧛ᓆ↵ 4,㉿ቝ᣿ర 1, ‐႐Ả৻ 1,4,5
1

ᘮᙥ⟵Ⴖᄢቇකቇㇱ࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦකቇᢎቶ‫ޔ‬᧲੩ㇺᣂኋ඙ାỚ↸ 352ᘮᙥ⟵Ⴖᄢቇᄢቇ㒮ℂᎿቇ⎇ⓥ⑼‫ޔ‬␹ᄹᎹ⋵ᮮᵿᏒ᷼ർ඙ᣣศ 3-14-1
3᧲ᶏᄢቇකቇㇱኾ㐷⸻≮ቇ♽࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦ⑼ቇ‫ޔ‬␹ᄹᎹ⋵દ൓ේᏒਅ♯ደ 143‫ޔ‬4ᘮᙥ⟵Ⴖᄢቇ᦬ࡩἑ࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦ࠮ࡦ࠲࡯‫ޔ‬㕒ጟ⋵દ⼺Ꮢ᦬ࡩἑ 380-2
5ᘮᙥ⟵ႶᄢቇℂᎿቇㇱ↢๮ᖱႎቇ⑼‫ޔ‬␹ᄹᎹ⋵ᮮᵿᏒ᷼ർ඙ᣣศ 3-14-1
email: ushiyama@bme.bio.keio.ac.jp

Keywords: ⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬ㧘⟲ൻ᡼㔚㧘βᏪⷐᣦ‫ޣ‬⢛᥊‫ޤ‬ࡅ࠻ߩ㓐ᗧㆇേߪ㧘ᄢ⣖⊹⾰৻ᰴ૕ᕈᗵⷡㆇേ㊁㧔ㆇേ⊹⾰㧕ࠃࠅ⊒ߖࠄࠇࠆㆇേᜰ઎߇⣄㜑ౝㆇേ࠾ࡘ࡯ࡠࡦࠍ㚟േߐߖ㧘╭෼❗ࠍᒁ߈⿠ߎߔߎߣߢ⊒⃻ߔࠆ㧚᡿ߦ㧘ࡅ࠻ߩㆇേ⊒⃻࡮೙ᓮߩࡔࠞ࠾࠭ࡓࠍ⸃᣿ߔࠆߚ߼ߦߪ㧘⣖࡟ࡌ࡞࡮⣄㜑࡟ࡌ࡞ߩ␹⚻ᵴേߣㆇേ಴ജߣߩ࿃ᨐ㑐ଥࠍ൮᜝⊛ߦℂ⸃ߔࠆᔅⷐ߇޽ࠆ㧚ᒙгਛᒝᐲߩ㕒⊛╭෼❗ਛ㧘ㆇേ⊹⾰ㄭறߩ㗡⊹਄߆ࠄዉ಴ߒߚ⣖ᵄ㧔EEG㧕ߣਥ௛╭ߩ╭㔚࿑㧔EMG㧕ߦߪ㧘15-35 Hz Ꮺၞ㧔βᏪ㧕ߩࠃߊૃߚࡄ࠲࡯ࡦߩᵄᒻ߇
ⷰኤߐࠇࠆ㧚1995 ᐕߦ Halliday ࠄߦࠃߞߡ⠨᩺ߐࠇߚࠦࡅ࡯࡟ࡦࠬ⸃ᨆᴺߪ㧘ᓞേ⊛ߥਔᵄᒻߩ⋧㑐ᕈࠍ⹏ଔߔࠆߎߣߢ㧘ㆇേ♽ߩᦨ⚳಴ജߣߒߡߩ╭᡼㔚ࠍㆇേ⊹⾰߇޿߆ߦࠦࡦ࠻ࡠ࡯࡞ߒߡ޿ࠆ߆ࠍቯ㊂⹏ଔߔࠆ㕖ଚⷅ⊛ᚻᴺߣߒߡᵈ⋡ࠍ㓸߼ߡ޿ࠆ㧚ᧄ⎇ⓥߪ㧘╭∋ഭߦ઻޿㧘ߎߩ⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬ߇ߤߩ
ࠃ߁ߦᄌൻߔࠆ߆ࠍቯ㊂⹏ଔߔࠆߎߣߦࠃࠅ㧘╭∋ഭ⵬ఘᯏ᭴ߣߒߡߩㆇേ⊹⾰г╭㑆ߩᵴേㅪ㑐ߩᗧ⟵ࠍ޽߈ࠄ߆ߦߔࠆߎߣࠍ⋡⊛ߦၫࠅⴕࠊࠇߚ㧚‫ޣ‬ᣇᴺ‫ޤ‬ⵍᬌ⠪ߪஜᏱߥᚑੱ↵ሶ㧣ฬ㧔ᐕ㦂㧘22-25 ᱦ㧕ߢ޽ߞߚ㧚EEG ߪ㧘ਅ⢇╭⟲
ࠍᡰ㈩ߔࠆㆇേ⊹⾰㗔ၞ߇૏⟎ߔࠆ㗡㗂ㇱ㧔Cz㧕਄㧘߅ࠃ߮ߘߩᏀ 2cm ਄ߦ⋁㔚ᭂࠍ⟎߈㧘෺ᭂዉ಴ߒߚ㧔ࡃࡦ࠼ࡄࠬ㧘0.1-200 Hz㧕
㧚EMG ߪ㧘ฝ஥೨⣊㛽╭ߩ╭⣻਄ߦ㔚ᭂ㑆〒㔌 2cm ߢ߰ߚߟߩ㔚ᭂࠍ⟎߈㧘෺ᭂዉ಴ߒߚ㧔ࡃࡦ࠼ࡄࠬ㧘
0.5-1000 Hz㧕㧚߹ߚ㧘⿷㑐▵⢛ዮᒛജ㧔Force㧕ࠍ㧘╭ജ⸘ߦߣࠅߟߌߚࠬ࠻࡟ࠗ
ࡦࠥ࡯ࠫࠃࠅ⸘᷹ߒߚ㧚ⵍᬌ⠪ߪ㧘ታ㛎↪ߩ᫹ሶߦ⤒㑐▵ⷺᐲ 90 ᐲ㧘⿷㑐▵ᐩ⢛ዮ 0 ᐲߩᆫ൓ߢᐳࠅ㧘ฝ⿷ࠍ╭ജ⸘ߦ࿕ቯߒߚ㧚Pre ⺖㗴ߣߒߡ㧘ᦨᄢദജ㧔Maximal voluntary contraction,
MVC㧕ߩ 30%ߩ㕒⊛⿷⢛ዮㆇേࠍ 60 ⑽㑆ⴕࠊߖߚ㧚ⵍᬌ⠪ߩ೨ߦ⸳⟎ߒߚࠦࡦࡇࡘ࡯࠲ࠬࠢ࡝࡯ࡦߦߪ㧘Force ߅ࠃ߮⋡ᮡ Force ࡟ࡌ࡞ࠍឭ␜ߒ㧘ⵍᬌ⠪ߦߪ⺖

－1－㗴ࠍㅢߓߡ㧘ߢ߈ࠆߛߌᱜ⏕ߦForce ࠍ⋡ᮡ࡟ࡌ࡞ߦ޽ࠊߖࠆࠃ߁ߦᜰ␜ࠍߒߚ㧚ߘߩᓟ㧘Fatigue ⺖㗴ߣߒߡ㧘 50%MVC ߩᒝᐲߢߩ㕒⊛⿷⢛ዮㆇേࠍ∋ഭ࿎ᘻ߹ߢⴕࠊߖ㧘╭∋ഭࠍ⺃⊒ߐߖߚ㧚⊒ើᒛജ߇ 5 ⑽㑆 40%MVC ࠍਅ࿁ߞߚߣ߈㧘∋ഭ࿎ᘻߦ⥋ߞߚߣ್ᢿߒߚ㧚Fatigue ⺖㗴ᓟ㧘ߚߛߜߦ Post ⺖㗴ߣߒߡ㧘Pre ⺖㗴ߣห᭽ߩ࠲ࠬࠢࠍⵍᬌ⠪ߦⴕࠊߖߚ㧚‫⚿ޣ‬ᨐ‫ޤ‬࿑ 1 ߦߪ㧘Pre ⺖㗴㧘Post ⺖㗴ߘࠇߙࠇߦ߅ߌࠆ EEG㧘EMG㧘Force ߩ↢ᵄᒻ㧘
EEG㧘ᢛᵹ EMG㧘Force ߩࡄࡢ࡯ࠬࡍࠢ࠻࡞㧔PSD㧕
㧘EEG ߣᢛᵹ EMG 㑆ߩࠦࡅ࡯࡟ࡦࠬࠬࡍࠢ࠻࡞ࠍߘࠇߙࠇ␜ߔ㧚╭∋ഭ⺃⊒ᓟ㧘EMG ߩβᏪᓞേ㧔⟲ൻ᡼㔚㧕߇㗼⪺ߦ਄᣹ߔࠆߎߣ߇ⷰኤߐࠇ㧘EEG ห᭽㧘EMG ߩ PSD ਄ߦ߽㧘⪺᣿ߥβᏪࡇ࡯ࠢ߇಴⃻ߒߚ㧚ߎߩߣ߈㧘ห᭽ߩ๟ᵄᢙᏪ㧔βᏪ㧕ߦ߅޿ߡ㧘⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߩࡇ࡯ࠢ߇㗼⪺ߦჇടߒߚ㧚ߎ߁ߒߚ⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߩჇട
ߪ㧣ฬਛ 6 ฬߩⵍᬌ⠪ߢⷰኤߐࠇ㧔࿑ 2A㧕㧘βᏪߦߺࠄࠇࠆࠦࡅ࡯࡟ࡦࠬߩࡇ࡯
ࠢ୯㧔Cohmax㧕߅ࠃ߮᦭ᗧߣ್ቯߐࠇߚࠦࡅ࡯࡟ࡦࠬࠬࡍࠢ࠻࡞ߩ㕙Ⓧ㧔Coharea㧕ࠍ▚಴ߔࠆߎߣߢ㧘Pre-Post 㑆ߩᏅ⇣ࠍ⛔⸘⊛ߦᲧセߒߚ⚿ᨐ㧘޿ߕࠇߩᜰᮡߦ
߅޿ߡ߽╭∋ഭߦࠃࠆ᦭ᗧߥჇട߇ᬌ಴ߐࠇߚ㧔Cohmax: Pre, 0.114 ± 0.005; Post,
0.344 ± 0.091; P = 0.004㧕
㧔Coharea: Pre, 0.247 ± 0.279; Post, 0.802 ± 0.531; P = 0.032㧕㧚߹ߚ㧘࿑ 1 ߦ㗼⪺ߥࠃ߁ߦ㧘ߎ߁ߒߚ␹⚻ᵴേߩᄌൻߣߣ߽ߦ㧘Force㧔࿑ 2B㧕
ߩំࠄ߉ߩჇട߽ⷰኤߐࠇߚ㧚Force ߩំࠄ߉ࠍᄌേଥᢙ㧔ForceCV㧕ࠃࠅ⹏ଔߒ㧘
Pre-Post 㑆ߢߩᲧセࠍ߅ߎߥߞߚ⚿ᨐ㧘╭∋ഭߦࠃࠆ ForceCV ߩ᦭ᗧߥჇട߇ᬌ
಴ߐࠇߚ㧔Pre, 3.24 ± 1.18 %; Post, 6.42 ± 3.36 %; P = 0.020㧕
㧔࿑ 3㧕
㧚 ߎ߁ߒߚ㧘╭∋ഭߦ઻߁␹⚻ᵴേ߿ㆇേ಴ജߩᄌൻߩᤨ㑆⚻ㆊࠍࠃࠅ⹦⚦ߦᬌ⸛ߔࠆߚ߼ߦ㧘Fatigue ⺖㗴ਛߩᤨ㑆ᄌൻߦߟ޿ߡ߽޽ࠊߖߡቯᕈ⊛ߦⷰኤߒߚ㧚࿑ 4 ߦߪ㧘Fatigue ⺖㗴ਛߩ EMG ߅ࠃ߮ Force ߩ↢ᵄᒻ㧘EMG ߩੑਸ਼ᐔဋᐔᣇᩮ㧔EMGRMS㧕߅ࠃ߮ᐔဋ๟ᵄᢙ㧔EMGMPF㧕
㧘ForceCV㧘⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦ
ࠬߩ Time-Frequency Map㧔⊕ߊߥࠆ߶ߤࠦࡅ࡯࡟ࡦࠬߩᒝᐲ߇Ⴧടߒߚߎߣࠍ␜
ߔ㧕ࠍ␜ߔ㧚ߎߎߢ↪޿ߚ EMGRMS㧘EMGMPF ߪ╭∋ഭߦࠃࠆ╭ᵴേࠬ࠻࡜࠹
ࠫ࡯ߩᄌൻࠍ⹏ଔߔࠆᜰᮡߢ޽ࠅ㧘೨⠪ߪേຬߐࠇࠆㆇേන૏㧔߭ߣߟߩ⣄㜑ౝㆇേ࠾ࡘ࡯ࡠࡦߣߘߩᡰ㈩ߔࠆ╭✢⛽⟲ߩߎߣ㧕ߩᢙߩᄌൻࠍ㧘ᓟ⠪ߪേຬߐࠇ
ߚㆇേන૏ߩ⊒Ἣ㗫ᐲߩᄌൻߩᜰᮡߣߒߡ↪޿ࠄࠇߡ޿ࠆ㧚Fatigue ⺖㗴㐿ᆎ⋥ᓟ߆ࠄ㧘ਔᜰᮡߪߘࠇߙࠇჇട㧘ᷫዋࠍߒߡ޿ߊ߇㧘Fatigue ⺖㗴ਛ⋚ߦ߅޿ߡਔᜰ
ᮡߩᄌൻߪ㗡ᛂߜߣߥߞߡ޿ࠆ㧚⥝๧ᷓ޿ߎߣߦ㧘ߎࠇࠄߩᄌൻ߇㗡ᛂߜߦߥߞ
ߚ⋥ᓟ߆ࠄ⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߩβᏪᒝᐲ਄᣹ߒ㧘ߘࠇߦ઻޿ ForceCV ߩჇട߽⿠ߎߞߡ޿ߚ㧚‫ޣ‬⠨ኤ‫ޤ‬ᧄ⎇ⓥߩ⚿ᨐ㧘㕒⊛╭෼❗ߦࠃࠅ╭∋ഭ߇⺃⊒ߐࠇߚ᧦ઙਅߦ߅޿ߡ㧘⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߪ᦭ᗧߦჇᄢߔࠆߎߣ߇᣿ࠄ߆ߣߥߞߚ㧚߹ߚ㧘Fatigue ⺖㗴ਛ㧘⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߣ EMGRMS ߿ EMGMPF ߩᤨ㑆ᄌൻߣߩ㑆ߦߪᄢ߈ߥ
ᤨ㑆Ꮕ߇ⷰኤߐࠇߚߎߣ߆ࠄ㧘ㆇേ⊹⾰г╭㑆ߩㅪ㑐Ⴧᒝߪ㧘ㆇേන૏ߩേຬ߅ࠃ
߮⊒Ἣ㗫ᐲߩ⺞ᢛߦᰴߋ㧘╭∋ഭ⵬ఘߩߚ߼ߩ╙ਃߩ╭ᵴേࠬ࠻࡜࠹ࠫ࡯ߢ޽ࠆ
ߎߣ߇␜ໂߐࠇߚ㧚߹ߚ㧘╭∋ഭᓟߩ⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߩ᦭ᗧߥ਄᣹ߦ઻
޿㧘EMG ߦߺࠄࠇࠆ⟲ൻ᡼㔚߽⪺᣿ߦߥࠅ㧘޽ࠊߖߡ Force ߩំࠄ߉߽᦭ᗧߦჇ
ടߒߚ㧚ߎߩߎߣߪ╭∋ഭ⊒⃻ᤨ㧘ਛᨔ␹⚻♽ߪㆇേ⊹⾰߆ࠄߩਅⴕᕈ౉ജࠍࠃ
ࠅᓞേ⊛ߦߒ㧘╭ߩ⟲ൻ᡼㔚ࠍߟߊࠅߛߔߎߣߢ㧘⊒ើᒛജߩ቟ቯᕈࠍ‶†ߦߒߟߟ߽㧘৻ቯᒛജ଻ᜬࠍߤ߁ߦ߆ታ⃻ߒߡ޿ࠆߎߣ߇␜ໂߐࠇߚ㧚 ߥ߅㧘ᧄ⎇ⓥߩౝኈߪ㧘Journal of Applied Physiology 110: 1233-1240, 2011 ߦឝタᷣߩ߽ߩߢ޽ࠆ㧚⹦⚦ߦߟ޿ߡߪߘߜࠄࠍෳᾖߐࠇߚ޿㧚－2－੹ᓟߩዷᦸ
⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߪ㧘ߘߩ⸃ᨆᚻᴺ߇⠨᩺ߐࠇߡ⚂ 15 ᐕ㧘ᄙߊߩ␹⚻↢ℂ⎇ⓥߦ߅޿ߡ↪޿ࠄࠇ㧘ߘߩ⊒⃻ࡔࠞ࠾࠭ࡓ߿ᯏ⢻⊛ᗧ⟵ߦߟ޿ߡ⼏⺰߇➅ࠅᐢߍࠄࠇߡ߈ߚ㧚ߣߊߦ㧘ㄭᐕ㧘ᄙߊߩᮮᢿ⊛⎇ⓥߦ߅޿ߡ㧘∛ᱧ㧘ㆇേ┹ᛛᱧ㧘ട㦂ߥߤߦ઻߁ᓟᄤ⊛ᄌൻߦߟ޿ߡႎ๔߇ߥߐࠇߡ߅ࠅ㧘ㆇേᯏ⢻ߣߩᷓ޿㑐ࠊࠅ߇␜ໂߐࠇߡ޿ࠆ㧚ᧄ⎇ⓥߢߪ㧘╭∋ഭߦ઻߁⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߣ⊒ើᒛജߩᄌൻߣߩኻᲧ߆ࠄ㧘╭∋ഭ⊒⃻ᤨߩ␹⚻♽ᯏ⢻ߩᄌൻ㧘߅ࠃ߮ߘߩㆇേ಴
ജ߳ߩᓇ㗀ࠍ൮᜝⊛ߦߣࠄ߃ࠆߎߣࠍ⹜ߺߚ߇㧘ߎ߁ߒߚ㔚᳇↢ℂ࡮ㆇേቇ෺ᣇ
߆ࠄߩࠕࡊࡠ࡯࠴ࠍ⚵ߺวࠊߖߚ⎇ⓥߪታߪ㛳ߊ߶ߤዋߥ޿㧚੹ᓟ߽㧘ࡅ࠻ߩ⣖࡮り૕ࠍ߭ߣߟߩࠪࠬ࠹ࡓߣߒߡᝒ߃㧘ߐ߹ߑ߹ߥㆇേ⺖㗴㧘޽ࠆ޿ߪㆇേቇ⠌ߦࠃࠆ⣖ᵄ╭㔚࿑ࠦࡅ࡯࡟ࡦࠬߩᄌൻ㧘߅ࠃ߮ߘߩㆇേ಴ജ߳ߩᓇ㗀ࠍᬌ⸛ߔࠆߎ
ߣߢ㧘ㆇേ⊹⾰г╭㑆ߩᵴേㅪ㑐߇߽ߟᯏ⢻⊛ᗧ⟵ߦߟ޿ߡㄼߞߡ޿߈ߚ޿ߣ⠨߃ߡ޿ࠆ㧚      

࿑ 㪈㩷

－3－

࿑ 㪊㩷

࿑ 㪉㩷

࿑ 㪋㩷

－4－

ࡑ࡜࠰ࡦ࡜ࡦ࠽࡯߆ࠄߩᖱႎ࠮ࡦࠪࡦࠣ⮮↰ ᖗᴺ᡽ᄢቇ ᖱႎ⑼ቇㇱ᧲੩ㇺዊ㊄੗Ꮢ᫃㊁↸ 3-7-2
email: fujita_s@hosei.ac.jp
Keywords: ࡑ࡜࠰ࡦ‫ޔ‬GPS‫ޔ‬ᔃᜉ‫ޔࡦࠜࡈ࠻࡯ࡑࠬޔ‬᳇⽎ⷐᣦࡑ࡜࠰ࡦᗲᅢኅߩᏒ᳃࡜ࡦ࠽࡯ߪ‫ޔ‬ᣣ‫ޔߢࠣࡦ࠾࡯࡟࠻ߩޘ‬หߓࠦ࡯ࠬࠍ➅ࠅ㄰ߒ⿛ⴕߒߚࡠࠣ )25‫ޔ‬ᔃᜉ࠺࡯࠲ߥߤ ࠍᄢ㊂ߩ࠮ࡦࠨᖱႎߣߒߡ଻᦭ߔࠆ‫ᧄޕ‬ႎ๔ߢߪ‫  ⚂ޔ‬ᐕ㑆ߦᷰࠆ  ࿁ߩ⿛ⴕࡠࠣࠍ෼㓸ߒߚ‫ࠍࠣࡠߩߎޔߡߒߘޕ‬ಽᨆߔࠆߎߣߢ‫⿛ޔ‬⠪ߩ․ᕈࠍಽᨆߢ߈ࠆߛߌߢߥߊ‫ޔ‬࿾ᒻ ᮡ㜞 ߿᳇⽎ᖱႎ ᳇᷷‫ޔ‬㘑ㅦ ࠍ޽ࠆ࡟ࡌ࡞ߢ㘃ផߢ߈ࠆߎߣࠍ␜ߒߚ‫ࡦ࠰࡜ࡑޔߚ߹ޕ‬ᄢળߦߟ޿ߡ߽‫ޔ‬ࠬࡑ࡯࠻ࡈࠜࡦߥߤߩ࠮ࡦࠨ㘃ࠍⵝ⌕ߔࠆߎߣߦࠃࠅ‫ޔ‬㐳ᤨ㑆ߩᔃᜉ‫ޔ‬ㅦᐲ‫ޔ‬⣨߰ࠅߩᄌൻߩ⋧㑐ߣ޿߁⿛⠪ߩ․ᕈ߿‫ੱߩߡߒߣⴐ⟲ޔ‬㑆ⴕേߩ᛽಴‫ޔ‬%1 Ớᐲ࠮ࡦࠨߦࠃࠆᴪ㆏ⅣႺߩ⺞ᩏߥߤ‫ޔ‬᭽‫ࠍ࠲࡯࠺ߥޘ‬෼㓸ߢ߈ࠆߎߣࠍ␜ߒߚ‫ޕ‬੹ᓟߩዷᦸ
੹࿁ߩႎ๔ߪ‫ߩߡ޿ߟߦ࡯࠽ࡦ࡜ߩੱ৻ޔ‬ಽᨆߢ޽ࠆ߇‫ޔ߫߃ߣߚޔ‬ㅢൕ࡮ㅢቇ
ߩࠃ߁ߥᄙੱᢙ߇‫ޔ‬หߓࠃ߁ߥ↢ᵴࠍ⛯ߌߡ‫ޔ‬ᄢ㊂ߩࡠࠣࠍᱷߔߎߣ߇ߢ߈ࠇ߫‫ޔ‬ⴝⷺߩᲤᣣߩᄌൻࠍᄢ㊂ߩ࠮ࡦࠨᖱႎ߆ࠄ⊒⷗ߢ߈ࠆߩߢߪߥ޿߆ߣ⠨߃ࠆ‫ޕ‬࿾ᒻ⊛ߩࡒࠢࡠߥᄌൻߥߤ‫⥝ޔ‬๧ᷓ޿․ᕈ࠺࡯࠲ߩ᛽಴ߦߟߥߍߚ޿ߣ⠨߃ࠆ‫ޔߚ߹ޕ‬⟲ⴐߩⴕേߣ޿߁ⷰὐ߆ࠄߪ‫ߩੱޔ‬േ߈‫ߩੱޔ‬ᵹࠇࠍนⷞൻߔࠆߎߣߪ‫ࠬࡀࠫࡆޔ‬⊛ߥⷰὐߢ߽ଔ୯ߩ㜞޿ᖱႎࠍ↢ߺ಴ߔน⢻ᕈ߇޽ࠆ‫ޕ‬એ਄ߩࠃ߁ߦ‫↢ޔ‬ᵴߦኒ⌕
ߒߚᄢ㊂ߩ࠮ࡦࠨᖱႎߩ෼㓸‫ޔ‬ಽᨆ‫ޔ‬ᵴ↪ߣ޿߁⎇ⓥߦߟߥߍߚ޿ߣ⠨߃ߡ޿ࠆ‫ޕ‬－5－䝬䝷䝋䞁䝷䞁䝘䞊䛛䜙䛾᝟ሗ䝉䞁䝅䞁䜾ἲᨻ኱Ꮫ㻌 ᝟ሗ⛉Ꮫ㒊㻌 ⸨⏣ ᝅ◊✲䛾┠ⓗᕷẸ䝷䞁䝘䞊䛾≉ᚩᕷẸ䝷䞁䝘䞊䛾᪥䚻䛾䝖䝺䞊䝙䞁䜾䝻䜾䜔䚸኱఍䛾㉮⾜䝻䜾䜢཰㞟䛧䚸䝷䞁䝘䞊䛾≧ែ䜔࿘㎶⎔ቃ䜢ศᯒ䛩䜛• ྠ䛨䝁䞊䝇䜢ẖ᪥㉮䜛• ⮬ศ䛾㉮⾜グ㘓䜢䝕䞊䝍໬䛩䜛䛣䛸䜢ዲ䜐• ᕷ㈍䛾GPS ௜䛝⭎᫬ィ䜢ᙜ䛯䜚๓䛾䜘䛖䛻ᣢ䛴(1) ㏿ᗘ䚸ᚰᢿ䚸ᶆ㧗䛾ᖹᆒⓗ䝟䝍䞊䞁䝖䝺䞊䝙䞁䜾
ᑐ㇟⪅: ྠ୍䛾⿕㦂⪅⾜ື: ᪥䚻䛾䝷䞁䝙䞁䜾䝖䝺䞊䝙䞁䜾㉳అ䛾䛒䜛10km䛾ᕷ⾤ᆅ䝁䞊䝇䜢
1-4࿘㉮⾜ィ ᶵჾ: Garmin♫ ForeAthlete 305
GPS䛸ᚰᢿィィ ᮇ㛫: 2008/10/26 䡚2011/8/29
118᪥ศ䛾䝕䞊䝍ᖹᆒ4⛊䛻1ᅇ⛬ᗘ䛾 ᐃ118᪥䛾඲䝕䞊䝍䜢䜎䛸䜑䛶ศᯒ䛩䜛䛸ᵝ䚻䛺⤖ᯝ䛜ぢ䛘䛶䛟䜛• ᶆ㧗䛿ᅜᅵᆅ⌮㝔䛾ᆅᅗ䛸୍⮴• ㉮⾜㏿ᗘ䜒䚸ᚰᢿᩘ䜒ྠ䛨䝟䝍䞊䞁䜢⧞䜚㏉䛩• ㉮⾜㏿ᗘᕪ䛸ᚰᢿᩘᕪ䛜㈇䛾┦㛵• Ẽ㇟䛸䛾┦㛵䜒ഹ䛛䛺䛜䜙ほ• 㢼㏿䚸Ẽ 䛸䛾┦㛵⥳⥺: ᶆ㧗
㟷⥺: ㏿ᗘ㉥⥺: ᚰᢿᩘ

(2) ㏿ᗘ䚸ᚰᢿᩘ䚸ഴᩳ䛾┦㛵ಀᩘ
㏿ᗘ
㏿ᗘ
ᚰᢿᩘ

0.495

ഴᩳ

-0.296

ᚰᢿᩘ

ഴᩳ

0.495

-0.2960.02760.0276(3) ㏿ᗘ䛾ᖹᆒᕪ䚸ᚰᢿ䛾ᖹᆒᕪ䚸ഴᩳ䛾┦㛵ಀᩘ
㏿ᗘࡢᕪ

m

ᚰᢿᩘࡢᕪ

-0.351ഴᩳ-0.489

beats
/ sec

ᶆ
Heartbeat㧗
Lap Timeഴᩳ-0.351-0.4890.212

0.212
㟷⥺: ໭㢼3m ௨ୖ䛾᪥䛾ᖹᆒᚰᢿᩘ/㏿ᗘ㉥⥺: ༡㢼3m ௨ୖ䛾᪥䛾ᖹᆒᚰᢿᩘ/㏿ᗘ⥳⥺: ඲䝕䞊䝍䛾ᖹᆒᚰᢿᩘ/ ㏿ᗘ(4) ໭㢼䛾ᙳ㡪

km/h

m

ᚰᢿᩘࡢᕪ

໭᪉ྥ䜈䛾
㉮⾜

໭᪉ྥ䜈䛾
㉮⾜

min

km

㉮⾜㊥㞳

Velocity

AltitudeHeartbeat
Velocity㏿ᗘࡢᕪ

118᪥ศ䛾䝕䞊䝍䛛䜙ồ䜑䛯ᶆ㧗୍᪥䛾䝕䞊䝍䜢ぢ䛶䜒䚸ഴྥ䛿䛴䛛䜑䛺䛔
m

㉮⾜㊥㞳

km

⭎䜅䜚䛾ຍ㏿ᗘᮾி䝬䝷䝋䞁

1.5Hzᐃ⤖ᯝ• య䛜෭䛘ษ䛳䛶㏿ᗘపୗ䛧䛯᫬䛻䛿ᚰᢿᩘ䜒⃭ῶ• ⭎䜅䜚ᅇᩘ(= Ṍᩘ)䜒䛣䜜䛻㐃ື• ᮾி䛾஧㓟໬Ⅳ⣲⃰ᗘ䛾ศᕸ䛻䛴䛔䛶䛿᫂☜䛺䝕䞊䝍䜢ᚓ䜙䜜䛪

FFT2010ᖺ2᭶28᪥㻌 ኳẼ䠖䜏䛮䜜ᚰᢿィCO2䝉䞁䝃
䞉CO2⃰ᗘ
䞉ẼAndroid ➃ᮎ• GPS• ⭎䛾ຍ㏿ᗘ• ⭎䛾ഴ䛝

஧㓟໬Ⅳ⣲⃰ᗘ(⎔ቃ)

㉮⾜㏿ᗘ䛸ᚰᢿᩘ

raincoatGPS䛴䛝⭎᫬ィ• GPS• ㉮⾜㏿ᗘ

ὀ: ෗┿䛿䜲䝯䞊䝆䝉䞁䝃᝟ሗ䛾ඹ᭷(㥴ᗓ䝬䝷䝋䞁)㛵㐃◊✲: ⩌⾗䝅䝭䝳䝺䞊䝅䝵䞁ᐇ㦂ෆᐜ• 4ே䛾⿕㦂⪅䛜Android➃ᮎ䜢ᣢ䛴• GPS䛻䜘䜛㉮⾜㏿ᗘ䚸⭎䜅䜚䛾ຍ㏿ᗘ䜢 ᐃ3000ே䝅䝇䝔䝮ᴫせ• Android: 30⛊䛻1ᗘ䚸఩⨨䛸㏿ᗘ䜢㏦ಙ• Google App Engine: ཷಙ䛧䛯䝕䞊䝍䜢ಖᏑ䛧䚸
Google Maps ୖ䛻⾲♧• 䝤䝷䜴䝄: ⾲♧䛸ᛂ᥼䝯䝑䝉䞊䝆䛾㏦ಙ

1୓ே

－6－

⹺ቯㆮવࠞ࠙ࡦ࠮࡜࡯ߩ
ࠨࠗࠛࡦࠬࠦࡒࡘ࠾ࠤ࡯࠲࡯ߣߒߡߩᓎഀ
ጟፒᕺ೑‫⋧ޔ‬Ỉᒎ↢‫ޔ‬ṚỈ౏ሶ‫ޔ‬ቶફ߈ߺሶ‫ޔ‬Ꮉ⋡⵨߅⨥ߩ᳓ᅚሶᄢቇᄢቇ㒮 ੱ㑆ᢥൻഃᚑ⑼ቇ⎇ⓥ⑼࡜ࠗࡈࠨࠗࠛࡦࠬኾ᡹ ㆮવࠞ࠙ࡦ࠮࡝ࡦࠣࠦ࡯ࠬ
᧲੩ㇺᢥ੩඙ᄢႦ 2-1-1
email:takizawa.kimiko@ocha.ac.jp
Keywords: ⹺ቯㆮવࠞ࠙ࡦ࠮࡜࡯ ㆮવࠞ࠙ࡦ࠮࡝ࡦࠣ
ࠨࠗࠛࡦࠬࠦࡒࡘ࠾ࠤ࡯࡚ࠪࡦ ㆮવᢎ⢒ ㆮવᕈ∔ᖚⷐᣦࠊ߇࿖ߢߪ‫߇߽⺕ޔ‬ㆮવሶߦ㑐ଥߔࠆ∛᳇ߦ߆߆ࠆน⢻ᕈ߇޽ࠆߣ޿߁⹺⼂߇ᵻ޿‫߇ޘੱޕ‬ᱜ⏕ߥᖱႎࠍ⍮ࠅ‫ࠄ⥄ޔ‬⠨߃್ᢿߔࠆߚ߼ߦ⹺ቯㆮવࠞ࠙ࡦ࠮࡜࡯
㧔ㆮવࠞ࠙ࡦ࠮࡝ࡦࠣߩኾ㐷ኅ㧕߇ࠨࠗࠛࡦࠬࠦࡒࡘ࠾ࠤ࡯࠲࡯ߣߒߡᵴべߔࠆߎߣ߇ᦼᓙߐࠇࠆ‫ޕ‬੹ᓟߩዷᦸ ⹺ቯㆮવࠞ࠙ࡦ࠮࡜࡯ߪ‫ޔ‬ㆮવߦ㑐ߔࠆ໧㗴ߦᖠ߻ࠢ࡜ࠗࠛࡦ࠻ߩᡰេߛߌߢߪ
ߥߊ‫ߦੱߩ⥸৻ޔ‬ኻߒߡᱜ⏕ߥ⍮⼂ࠍવ߃ࠆࠨࠗࠛࡦࠬࠦࡒࡘ࠾ࠤ࡯࠲࡯ߣߒߡ‫ޔ‬∛㒮ߥߤߩක≮ᯏ㑐ߩߺߥࠄߕ‫ޔ‬࿾ၞ࡮ቇᩞ࡮ડᬺ࡮ⴕ᡽ߢߩᵴべ߽ᦼᓙߐࠇ߹ߔ‫ޕ‬ ੹ᓟߪ‫ࠆߥࠄߐޔ‬ㆮવቇߩㅴᱠߦࠃࠅ᭽‫ߥޘ‬ᖱႎ߇Ⴧ߃‫ޔ‬ක≮ߦ߅ߌࠆㆬᛯ⢇߽ᐢ߇ࠆߎߣ߇੍ᗐߐࠇ߹ߔ‫ߚ߹ޕ‬කቇߩㅴᱠߦࠃࠅ‫ޔ‬ㆮવሶߩᖱႎߢ∛᳇ߩ߆߆ࠅ
߿ߔߐߛߌߢߥߊ‫⮎ޔ‬೷ߩല߈߿ߔߐߥߤ߽ಽ߆ࠆࠃ߁ߦߥߞߡ߈ߡ޿߹ߔ‫ޕ‬ߎ߁ߒߚਛߢࠨࠗࠛࡦࠬࠦࡒࡘ࠾ࠤ࡯࡚ࠪࡦߩᔅⷐᕈ߇Ⴧߒ‫⹺ޔ‬ቯㆮવࠞ࠙ࡦ࠮
࡜࡯ߩ࠾࡯߽࠭Ⴧ߃ߟߟ޽ࠅ߹ߔ‫ޕ‬－7－⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈱䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈫䈚䈩䈱ᓎഀጟፒᕺ೑䇮⋧Ỉᒎ↢䇮ṚỈ౏ሶ䇮ቶફ䈐䉂ሶ䇮Ꮉ⋡⵨䈍⨥䈱᳓ᅚሶᄢቇᄢቇ㒮㩷 ੱ㑆ᢥൻഃᚑ⑼ቇ⎇ⓥ⑼㩷 䊤䉟䊐䉰䉟䉣䊮䉴ኾ᡹㩷 ㆮવ䉦䉡䊮䉶䊥䊮䉫䉮䊷䉴䈲䈛䉄䈮㩷 ⑼ቇᛛⴚ䈱⊒ዷ䈮䉋䉍䇮↢ᵴ⠌ᘠ∛䈭䈬䈱৻⥸⊛䈭∛᳇䈮䉅ㆮવሶ䈏㑐ଥ䈜䉎น⢻ᕈ䈏䈅䉎䈖䈫䈏ಽ䈎䈦䈩䈐䈢䇯䈚䈎䈚䈭䈏䉌䇮⃻࿷䈱ᣣᧄ䈱ㆮવᢎ⢒䈪䈲䇮䊍䊃䈱ㆮવ䉇৻⥸⊛䈭∛᳇䈮䈧䈇䈩ቇ䈹ᯏળ䈏ዋ䈭䈒䇮⺕䉅䈏ㆮવሶ䈮㑐ଥ䈜䉎∛᳇䈮䈎䈎䉎น⢻ᕈ䈏䈅䉎䈫䈇䈉⹺⼂䈏ᵻ䈇䇯䈠䈱䈢䉄䇮䊍䊃ㆮવቇ䊶ㆮવකቇ䈱ᖱႎ䉕ᱜ⏕䈮⍮䉎ᔅⷐ䈏䈅䉍䇮䈠䉏䈮䈧䈇䈩⥄䉌⠨䈋್ᢿ䈜䉎ᔅⷐ䈏䈅䉎䇯㩷 㜞ᐲ䈪ኾ㐷⊛䈭ක≮଻ஜ䈮䈍䈔䉎ᖱႎ䉇వ┵⊛䈭ᴦ≮ᴺ䈭䈬䈱ᖱႎ䉕৻⥸䈱ੱ䇱䈮⊛⏕䈮ℂ⸃䈚䈩䈇䈢䈣䈒䈢䉄䈮䈲䇮䈠䉏䉌䈱⍮⼂䉕䈎䉂䈒䈣䈇䈩䇮䈎䈧ᱜ⏕䈮વ䈋䉎ੱ᧚䈏ᔅⷐ䈪䈅䉎䇯䈠䈱䈢䉄䈮䈲䇸⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䇹䈫䈇䈉ኾ㐷ኅ䈏ᓎ䈮┙䈧䈫⠨䈋䉌䈋䉎䇯⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈫䈲䇮䊍䊃ㆮવቇ䊶ㆮવකቇ䈭䈬䈮㑐䈜䉎ᷓ䈒ᄙ᭽䈭⍮⼂䉕ᜬ䈤䇮ㆮવ䈮㑐䈜䉎໧㗴䈮ᖠ䉃䉪䊤䉟䉣䊮䊃䈮ኻ䈚䈩䇮⋧ᚻ䈏ᔅⷐ䈫䈚䈩䈇䉎ᖱႎ䉕ಽ䈎䉍䉇䈜䈒⺑᣿䈜䉎䈖䈫䈏䈪䈐䇮ᔃℂ䊶␠ળ⊛䈭䉰䊘䊷䊃䉕ⴕ䈉䈖䈫䈏䈪䈐䉎ක≮଻ஜ䈱ኾ㐷⡯䈪䈅䉎䇯ᓥ䈦䈩䇮⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈱᏷ᐢ䈇ᓎഀ䈱䈵䈫䈧䈫䈚䈩䇮䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈱ᓎഀ䉕ᜂ䈇ᓧ䉎䇯㩷 ੹࿁䈲䇮⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈱䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈫䈚䈩䈱ᓎഀ䈮䈧䈇䈩ႎ๔䈜䉎䇯䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉲䊢䊮䈫䈲䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉲䊢䊮䋺⃻ઍ䈮䈍䈔䉎ኾ㐷ൻ䊶㜞ᐲൻ䈜䉎⑼ቇᛛⴚ䈮䈧䈇䈩৻⥸䈱ੱ䇱䈮䉒䈎䉍䉇䈜䈒વ䈋䇮৻⥸䈱ੱ䇱䈱⇼໧䉇ᗧ⷗䊶ⷐᦸ䈮ᔕ䈋䇮䈘䉌䈮᭽䇱䈭ኾ㐷ኅ䈮໧㗴䈱ᚲ࿷䉕વ䈋䉎䈫䈇䈉䇮෺ᣇะ䈱䉮䊚䊠䊆䉬䊷䉲䊢䊮 ♧ዩ∛䈫䈲䇮ⴊ♧୯䈏㜞䈒䈭䉎∛᳇䈪䈅䉎䇯ᵴേ䈪䈅䉎䇯
♧ዩ∛䈫㑐ଥ䈜䉎䈫䈘䉏䈩䈇䉎ㆮવሶ䈲䇮䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈫䈚䈩䈱⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䊶䈠䉅䈠䉅♧ዩ∛䈦䈩䈭䈮䋿䊶ㆮવሶᬌᩏ䈪ಽ䈎䉎䈦䈩⡞䈇䈢䉖䈣䈔䈬ᧄᒰ䋿৻⥸䈱ੱ䇱䈠䈱ㆮવሶ䉕ᜬ䈦䈩䈇䉎䈫ᔅ䈝∛᳇䈮䈭䉎䈱䈪䈲䈭䈒䇮∛᳇䈱䈎䈎䉍䉇䈜䈘䈮㑐ଥ䈜䉎ㆮવሶ䈪䈅䉎䇯♧ዩ∛䈫㑐ଥ䈜䉎䈫䈘䉏䈩䈇䉎ㆮવሶ䈲ⶄᢙ䈅䉍䇮⃻࿷⊒⷗䈘䉏䈩䈇䉎ㆮવሶ䈲䇮䈠䈱৻ㇱ䈪䈅䉎䇯䈠䈱䈢䉄䇮ㆮવሶᬌᩏ䈪⷗䈧䈎䈦䈢ㆮવሶ䈣䈔䉕䉂䈩䉅䇮䈠䈱♧ዩ∛䈮䈎䈎䉍䉇䈜䈇䈎䈬䈉䈎䉕್ᢿ䈜䉎䈖䈫䈏䈪䈐䈭䈇♧♧ዩ䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈏⑼ቇ⊛ᩮ᜚䈮ၮ䈨䈇䈢ᖱႎ䉕ឭଏ䉕䈜䉎䇯৻ᣇ⊛䈮ᖱႎឭଏ䉕䈜䉎䈱䈪䈲䈭䈒䇮⋧ᚻ䈏䈬䈉䈇䈉ᖱႎ䉇⹺⼂䉕䉅䈦䈩䈇䉎䈎䉕ᒁ䈐಴䈚䇮⋧ᚻ䈏᳞䉄䈩䈇䉎ᖱႎ䉕ឭଏ䈜䉎䇯

⎇ⓥ⠪

ᛛⴚ⠪

කᏧ 䈭䈬

䊍䊃䈱ㆮવቇ䊶ㆮવකቇ䈮䈧䈇䈩䇮䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈫䈚䈩ㆮવ䉦䉡䊮䉶䊤䊷䈏ᵴべ䈪䈐䉎⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈫䈲⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈱䉅䈩䉎⢻ജ䉕චಽ䈮⊒ើ䈜䉎䈢䉄䈮䇮ᵴേ䈱ⅣႺ䉕ᢛ஻䈚䇮ᵴേ䉕ᡰេ䈚䇮䈠䈱⚿ᨐ䈫䈚䈩␠ળో૕䈱ㆮવ䊥䊁䊤䉲䊷䈱ะ਄䉕࿑䉎䈖䈫䉕⋡⊛䈫䈚䈩䇮䇮
2009ᐕ䈮

䉦䉡䊮䉶䊤䊷䋺⾰䈱㜞䈇⥃ᐥㆮવක≮䉕ឭଏ䈜䉎䈢䉄䈮䇮⥃ᐥㆮવኾ㐷ක䈫ㅪ៤䈚䇮⹺ቯㆮવ䉦㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 ㆮવ䈮㑐䈜䉎໧㗴䈮ᖠ䉃䉪䊤䉟䉣䊮䊃䉕េഥ䈜䉎䈫䈫䉅䈮䇮䈠䈱ᮭ೑䉕቞䉎ኾ㐷ኅ㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 䋨⹺ቯㆮવ䉦䉡䊮䉶䊤䊷೙ᐲⷙೣ䋩⹺ቯㆮવ䉦䉦䉡䊮䉶䊤䊷
᭽䇱䈭␠ળ⾗Ḯ䈮 䉦䉡䊮䉶䊥䊮䉫ᛛⴚ䉕ቇ䉖䈪䈇䉎
䈧䈇䈩ቇ䉖䈪䈇䉎
⥃ᐥᔃℂ䉕ቇ䉖䈪䈇䉎

ᔃℂ䊶␠ળ⊛
䉰䊘䊷䊃䉕ⴕ䈉䈖䈫䈏䈪䈐䉎ၮ␆කቇ䉕ቇ䉖䈪䈇䉎

䊍䊃䈱ㆮવቇ䊶ㆮવකቇ䉕ቇ䉖䈪䈇䉎వ┵ක≮ቇ䉕ቇ䉖䈪䈇䉎

⑼ቇ⊛ᩮ᜚䈱䈅䉎ᖱႎ䉕ឭଏ䈜䉎䈖䈫䈏䈪䈐䉎⋧ᚻ䈏ᔅⷐ䈫䈜䉎ᖱႎ䉕䉒䈎䉍䉇䈜䈒વ䈋䉎䈖䈫䈏䈪䈐䉎䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈫䈚䈩䈱ᓎഀ⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䉪䊤䉟䉣䊮䊃

⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈱䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈫䈚䈩䈱ᓎഀ⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈲䇮ኾ㐷ኅ䈫৻⥸䈱ੱ䉕䈧䈭䈓ᯅᷰ䈚䈱ᓎഀ

ኻ⹤䉕ㅢ䈚䈩
৻⥸䈱ੱ䇱䈱
⠨䈋䉇⇼໧䉕
⍮䉎䈖䈫䈏䈪䈐䉎

৻⥸䈱ੱ䇱䈏䊍䊃䈱ㆮવቇ䊶ㆮવකቇ䈮䈧䈇䈩䈱ℂ⸃䉕ᷓ䉄䇮ᵴ↪䈜䉎⢻ജ䉕䉅䈧䈢䉄䈮䇮ℂ⻠Ṷળ䉇䉶䊚䊅䊷䇮䊪䊷䉪䉲䊢䉾䊒䈭䈬䉕ㅢ䈛䈩䇮⻠␠ળ䈮䊐䉞䊷䊄䊋䉾䉪䈜䉎䈖䈫䈏䈪䈐䉎

䇸NPOᴺ
ᴺੱ㩷㩷 ㆮવ䉦䉡䊮䉶䊥䊮䉫䊶䉳䊞䊌䊮䇹䉕⸳┙䈚䉁䈚䈢䇯
㩷
⑳䈢䈤䈲㩷㩷
䈖䈱䉋䈉䈭ᵴേ䉕䈲䈛䉄䈩䈇䉁䈜䇯㩷㩷㩷ㆮવ䉦䉡䊮䉶䊤䊷䈫∛㒮䉇ડᬺ䈱㑆䉕㩷㩷䈧䈭䈑䉁䈜䇯㩷㩷
䂹᦬䈮䋱࿁⸳ቯ䈘䉏䈢⋧⺣ᣣ䈮䇮䇸⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䇹䈏䉪䊤䉟䉣䊮䊃䈱䈗⋧⺣ᣣ䉕ᜂᒰ䈚䉁䈜䇯㩷㩷ᢎ⢒⃻႐䈭䈬䈪䇮ㆮવ䈮㑐䈜䉎᝼ᬺ䉇⻠Ṷળ䉕䈍ᚻવ䈇䈚䉁䈜䇯㩷㩷䂹ㆮવ⃻⽎䉇㪛㪥㪘䈭䈬䈮䈧䈇䈩䇮䉒䈎䉍䉇䈜䈒䈩㕙⊕䈇᝼ᬺ䉇ታ㛎䉕⥌䈚䉁䈜䇯㩷㩷㩷䈠䈱䈾䈎䇮䈘䉁䈙䉁䈭ᵴേ䉕ⴕ䈦䈩䈇䉁䈜䇯⹦䈚䈒䈲㪟㪧䉕䈗ⷩ䈒䈣䈘䈇䇯㩷㪿㫋㫋㫇㪑㪆㪆㫎㫎㫎㪅㫅㫇㫆㪅㪾㪺㪅㫁㫇㫅㪅㫆㫉㪾㪆㫀㫅㪻㪼㫏㪅㪿㫋㫄㫃㩷㩷㪉㪇㪈㪇ᐕ䈮㩷
䇸ᣣᧄ⹺ቯㆮવ䉦䉡䊮䉶䊤䊷දળ䇹䇹㩷㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 䉅⸳┙䈘䉏䉁䈚䈢䇯⠨ኤ䈫዁᧪䈱ዷᦸ㩷 ⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈲䇮ㆮવ䈮㑐䈜䉎໧㗴䈮ᖠ䉃䉪䊤䉟䉣䊮䊃䈱េഥ䈣䈔䈪䈲䈭䈒䇮৻⥸䈱ੱ䈮ኻ䈚䈩ᱜ⏕䈭⍮⼂䉕વ䈋䉎䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉺䊷䈫䈚䈩䈱ᓎഀ䉅ᜂ䈉䈖䈫䈏䈪䈐䉁䈜䇯∛㒮䈭䈬䈱ක≮ᯏ㑐䈱䉂䈭䉌䈝䇮࿾ၞ䊶ቇᩞ䊶ડᬺ䊶ⴕ᡽䈪䈱ᵴべ䉅ᦼᓙ䈘䉏䉁䈜䇯㩷 ੹ᓟ䈲䇮䈘䉌䈭䉎ㆮવቇ䈱ㅴᱠ䈮䉋䉍᭽䇱䈭ᖱႎ䈏Ⴧ䈋䇮ක≮䈮䈍䈔䉎ㆬᛯ⢇䉅ᐢ䈏䉎䈖䈫䈏੍ᗐ䈘䉏䉁䈜䇯䉁䈢කቇ䈱ㅴᱠ䈮䉋䉍䇮ㆮવሶ䈱ᖱႎ䈪∛᳇䈱䈎䈎䉍䉇䈜䈘䈣䈔䈪䈭䈒䇮⮎೷䈱ല䈐䉇䈜䈘䈭䈬䉅ಽ䈎䉎䉋䈉䈮䈭䉍䈧䈧䈅䉍䉁䈜䇯䉰䉟䉣䊮䉴䉮䊚䊠䊆䉬䊷䉲䊢䊮䈱ᔅⷐᕈ䈏Ⴧ䈚䇮⹺ቯㆮવ䉦䉡䊮䉶䊤䊷䈱䊆䊷䉵䉅Ⴧ䈋䈧䈧䈅䉍䉁䈜䇯

－8－ࠦࡦࡇ࠹ࡦࠪࡐ࡯࠻ࡈࠜ࡝ࠝࠍᵴ↪ߒߚቇ⠌ᡰេߦ㑐ߔࠆ⠨ኤᣂ⋡⌀♿ 㐳ᴧ዁৻ ጊᩮାੑ ᮭ⮮ବᒾ ₹ᧁ᱄਽ 1㕍ጊቇ㒮ᄢቇ ࡅࡘ࡯ࡑࡦ࡮ࠗࡁࡌ࡯࡚ࠪࡦ⎇ⓥ࠮ࡦ࠲࡯‫ޔ‬᧲੩ㇺᷦ⼱඙ᷦ⼱ 
ጊᒻᄢቇ ᢎ⢒ડ↹ቶ‫ޔ‬ጊᒻ⋵ጊᒻᏒዊ⊕Ꮉ↸ 

email: maki.arame@a2en.aoyama.ac.jp

Keywords: ࠦࡦࡇ࠹ࡦࠪࡐ࡯࠻ࡈࠜ࡝ࠝ㧘ኾ㐷ኅ⢒ᚑ㧘ࡔ࠲⹺⍮⊛⍮⼂ⷐᣦᧄ⎇ⓥߪ ICT ࠪࠬ࠹ࡓࠍ೑↪ߒߚ᝼ᬺㆇ༡ߦࠦࡦࡇ࠹ࡦࠪࡐ࡯࠻ࡈࠜ࡝
ࠝࠍዉ౉ߒߚ႐วߩቇ⠌ᡰេߦ㑐ߔࠆ⠨ኤߢ޽ࠆ‫ߦ⋡⑼ޕ‬㐽ߓߡ޿ߚቇ⠌ᡰេࠍኾ㐷ኅ߳ߩ㆏╭ࠍ⠨ᘦߒߚᡰេߦ᜛ᒛߔࠆ㓙ߦ⠨ᘦߔߴ߈ⷐ࿃ࠍ᣿
ࠄ߆ߦߒߚ‫ޕ‬੹ᓟߩዷᦸቇ⠌ࡊࡠ࠮ࠬߢࠦࡦࡇ࠹ࡦࠪࡐ࡯࠻ࡈࠜ࡝ࠝࠍ೑↪ߒߚቇ↢ߪ‫⹺࠲ࡔޔ‬⍮⊛⍮⼂ߩਛߢ߽⥄Ꮖ⍮⼂߇㜞߹ࠅ‫ޔ‬⡯ᬺ⊛⊒㆐߳ߩᗧ᰼ߣࠦࡦࡇ࠹ࡦࠪ
ࡐ࡯࠻ࡈࠜ࡝ࠝߦࠃࠆቇ⠌ߦ⋧㑐߇⷗ࠄࠇߚ‫⎇ᧄޕ‬ⓥߢߪ㧘ࠦࡦࡇ࠹ࡦࠪ
ࡐ࡯࠻ࡈࠜ࡝ࠝߩឭ␜ᣇᴺߩ᦭ലᕈ߿‫⸳ߩࠝ࡝ࠜࡈ࠻࡯ࡐࠪࡦ࠹ࡇࡦࠦޔ‬⸘⹏ଔߦ㑐ߔࠆ᦭ലᕈ߹ߢߩᬌ⸽ߪᚑߐࠇߡ޿ߥ޿‫੹ޕ‬ᓟߪߎ߁ߒߚὐߦ
ߟ޿ߡᦝߦ⎇ⓥࠍㅴ߼ߡ޿߈ߚ޿‫ޕ‬－9－ǳȳȔȆȳǷȝȸȈȕǩȪǪǛ෇ဇƠƨ‫ܖ‬፼ૅੲƴ᧙Ƣǔᎋ‫ݑ‬ᣂ⋡⌀♿㪁䊶㐳ᴧ዁৻㪁㪁䊶ጊᩮାੑ㪁䊶ᮭ⮮ବᒾ㪁䊶₹ᧁ᱄਽㪁
㕍
ቇ㒮ᄢቇ
䉲 ⎇ⓥ
䉺
ᒻᄢቇ
㕍ጊቇ㒮ᄢቇ䊍䊠䊷䊙䊮䊶䉟䊉䊔䊷䉲䊢䊮⎇ⓥ䉶䊮䉺䊷㪁ጊᒻᄢቇ㪁ᧄ⎇ⓥ䈲㪠㪚㪫䉲䉴䊁䊛䉕೑↪䈚䈢᝼ᬺㆇ༡䈮䉮䊮䊏䊁䊮䉲䊘䊷䊃䊐䉤䊥䉥䉕ዉ౉䈚䈢႐ว䈱ቇ⠌ᡰេ䈮㑐䈜䉎⠨ኤ䈪䈅䉎䋮
⑼⋡䈮㐽䈛䈩䈇䈢ቇ⠌ᡰេ䉕ኾ㐷ኅ䈻䈱㆏╭䉕⠨ᘦ䈚䈢ᡰេ䈮᜛ᒛ䈜䉎㓙䈮⠨ᘦ䈜䈼䈐ⷐ࿃䉕᣿䉌䈎䈮䈚䈢䋮

ᄂᆮ૾ඥ

ᄂᆮƷᏑ୎
•㪼䊤䊷䊆䊮䉫䉕ታᣉ䈜䉎㓙䈮ᔅⷐਇนᰳ䈭ኾ㐷ኅ䈫䈚䈩㪌⡯⒳䈱ኾ㐷ኅ䉕ቯ⟵䈚䋬䈠䈱⢒ᚑ䉕ⴕ䈦䈩䈐䈢䋮㪈㪅 ኾ㐷ኅ䈫䈚䈩ᬺോ⚻㛎䈏䈅䉎␠ળੱะ䈔䈮䉮䊮䊏䊁䊮䉲䈱䉼䉢䉾䉪䉕දജ㗂䈐䋬䊜䉺⹺⍮⊛⍮⼂䈮㑐䈜䉎⹺⼂䉕⺞ᩏ㪉㪇㪇㪏ᐕᐲ䈎䉌䈲䋬ኾ㐷ಽ㊁䈗䈫䈮⇣䈭䉎䉮䊮䊏䊁䊮䉲䈮⌕⋡䈚䈢䉮䊮䊏ኾ㐷ಽ㊁䈗䈫䈮⇣䈭䉎䉮䊮䊏䊁䊮䉲䈮⌕⋡䈚䈢䉮䊮䊏•㪉㪇㪇㪏ᐕᐲ䈎䉌䈲䊁䊮䉲䊘䊷䊃䊐䉤䊥䉥೑ᵴ↪䈮ข䉍⚵䉖䈪䈇䉎㪅㪉 ኾ㐷ኅ⢒ᚑ䊒䊨䉫䊤䊛䈱ቇ⠌䊒䊨䉶䉴䈮䉮䊮䊏䊁䊮䉲䊘䊷䊃䊐䉤䊥䉥䉕⚵㪉㪅ኾ㐷ኅ⢒ᚑ䊒䊨䉫䊤䊛䈱ቇ⠌䊒䊨䉶䉴䈮䉮䊮䊏䊁䊮䉲䊘 䊃䊐䉤䊥䉥䉕⚵䉂ㄟ䉂䋬䊜䉺⹺⍮⊛⍮⼂䈮㑐䈜䉎⹺⼂䈫ቇ⠌ലᨐ䈫䈱⋧㑐䉕⺞ᩏǤȳǹȈȩǯǷȧȊ ȫ
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ǳȳȆȳȄ
ǹȚǷȣȪǹȈ

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ȗȭȇȥȸǵ

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ˁಮ୿

ἋἚ Ἴ
ἋἚὊἼὊ
ἮὊἛ

ID✚⺰䈱ቇ⠌▸࿐

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‫إ‬ԓ୿

‫ܖ‬፼ỾỶἛ૙஬ἰἝἷỴἽᅈ˟ʴӼƚƷᛦ௹ኽௐ•䉮䊮䊏䊁䊮䉲䈱⥄Ꮖ⹏ଔ䈲⚻㛎ᐕᢙ䈮Ყ଀䈜䉎⚿ᨐ䈫䈭䈦䈢䋮ἅὊἋᢃဇǤȳǹȈȩǯǿ

ἾἕἋὅἩἻὅ

ȡȳǿἳὅἑ ἼὅἂỾỶἛἻỶὅἳὅἑ Ἴὅἂ
ỴἁἉἹὅἩἻὅἳὅἑ Ἴὅἂ

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᧏ႆܱ଀ỼὅἻỶὅਦ‫ݰ‬

ϻ̊

঺ௐཋ

Ўௌ

ᚨᚘ

෇ѣ

# PCN[UKU& GUKIP

5ᐕᧂḩ

ಽᨆ
5.0

‫ݣ‬᩿ਦ‫ݰ‬& GXGNQRO GPV + O RNGOGPVCVKQP

⹏ଔ

⸳⸘
0.0

ᚸ̖

ታᣉ

' XCNWCVKQP

ᄂᆮƷႸႎ

㐿⊒

5ᐕએ਄
10ᐕᧂḩ
10ᐕએ਄
15ᐕᧂḩ
15ᐕએ਄

ᬌ⸽䈮↪䈇䈢䉮䊮䊏䊁䊮䉲㪈㪊㪊㗄⋡ᬌ⸽䈮↪
䈢 䊮 䊁䊮䉲
㗄⋡•⑼⋡䈱ᨒ䈮㐽䈛䈩䈇䈢ᡰេ䉕䋬䉮䊮䊏䉲䊘䊷䊃䊐䉤䊥䉥䉕ᵴ↪䈚䋬ኾ㐷ኅ䈻䈱㆏╭䉕⠨ᘦ䈚䈢ᡰេ䈮᜛ᒛ䈜䉎䈮䈲䈬䈱䉋䈉䈮䈚䈢䉌䉋䈇䈎䋿‫ܖ‬፼ȗȭǻǹưƷૅੲኽௐ•ቇ⠌䊒䊨䉶䉴䈪䉮䊮䊏䊁䊮䉲䊘䊷䊃䊐䉤䊥䉥䉕೑↪䈚䈢ቇ↢䈲䋬⥄Ꮖ⍮⼂䈏㜞䉁䉎䈫⹺⼂䈜䉎ഀว䈏㜞䈒䋬␠ળੱ䈫Ყ䈼䈩䉅㆖⦡䈭䈇⚿ᨐ䈫䈭䈦䈢䋮
3.5
3
25
2.5
2
1.5
1
0.5
0

ᓥ੐ᐕᢙ5ᐕએ਄
ᓥ੐ᐕᢙ5ᐕᧂḩቇ↢

⸳໧1έᘍᄂᆮƷჷᙸ

⸳໧2

⸳໧3

⸳໧4

⸳໧7

•㪝㫃㪸㫍㪼㫃 㩿㪈㪐㪎㪐㪀䉇㪟㪸㪺㫂㪼㫉㩿㪈㪐㪐㪏㪀䈲䋬䊜䉺⹺⍮䉕䇸䊜䉺⹺⍮⊛⚻㛎䇹䈫䇸䊜䉺⹺⍮⊛⍮⼂䇹䈮ಽ㘃䈚䈩䈇䉎䋮ȢȇȫƴǑǔЎௌኽௐ•਄ᷗ㩿㪉㪇㪇㪎㪀䈲⥄Ꮖ⍮⼂䈮䈧䈇䈩䇸ਥ૕䇹䉇䇸䉝䉟䊂䊮䊁䉞䊁䉞䇹䉕᭴▽䈜䉎䈖䈫⥄૕䉅䋬䇸ቇ⠌䇹䈫ᝒ䈋䉎ⷞᐳ䈏ᔅⷐ䈪䈅䉎䈖䈫䉕ᜰ៰䋮•䊝䊂䊦䈮䉋䉎ᬌ⸽䈱⚿ᨐ䋬䈇䈝䉏䈱䊝䊂䊦䉅⋧㑐䉕␜䈜⚿ᨐ䈫䈭䈦䈢䋮•⡯ᬺ⊛⊒㆐䈱⃻䉒䉏䈫⠨䈋䉌䉏䉎⾗ᩰขᓧ䈻䈱ᗧ᰼䈫䉮䊮䊏䊁䊮䉲䊘䊷䊃䊐䉤䊥䉥䈮䉋䉎ቇ⠌ലᨐ䈮⋧㑐䈏⷗䉌䉏䈢䋮
ȢȇȫᲫ

ᛦ௹᪮Ⴘ
⸳໧
␠ળੱ ቇ↢
1䋮 ⥄ಽ䈱ᵴേ䉕ᝄ䉍㄰䈦䈢䋮䂾䂾2䋮 ⥄ಽ䈱ᓧᗧ䈭䈖䈫䈏䉒䈎䈦䈢䋮䂾䂾3䋮 ⥄ಽ䈱⺖㗴䉕ᛠី䈜䉎䈖䈫䈏䈪䈐䈢䋮䂾䂾
4䋮 ⥄ಽ䈱ᚑᨐ䉕⏕⹺䈜䉎䈖䈫䈏䈪䈐䈢䋮䂾䂾
5䋮 䈖䉏䉁䈪䈱䉨䊞䊥䉝䈪᦭೑䈭ὐ䈏᣿䉌䈎䈮䈭䈦䈢䋮
䂾
䉏䉁
䉨
⺖ 䈏
䈭 䈢
6䋮 䈖䉏䉁䈪䈱䉨䊞䊥䉝䈪䈱⺖㗴䈏᣿䉌䈎䈮䈭䈦䈢䋮䂾
7䋮 ৻䈧৻䈧䈱ᵴേ䈮䈧䈇䈩䈭䈟ⴕ䈦䈩䈇䉎䈱䈎䉕⚊䂾䂾ᓧ䈜䉎䈖䈫䈏䈪䈐䈢8䋮 䈬䈱䉋䈉䈮䈜䉏䈳䉋䉍䉋䈒ᬺോ䈏ㆀⴕ䈪䈐䉎䈎䈫䈇䂾䈉䈖䈫䈏ಽ䈎䉎䉋䈉䈮䈭䈦䈢ȢȇȫᲬቴⷰ⊛䈮⷗⋥䈜䈖䈫䈏䈪䈐䈢ઁ䈱⢻ജ䈮䈧
䈇䈩䉅⹏ଔ䈚䈩
䉂䈢䈇ቇ⠌䈏䈜䈜䉄䉇䈜䈒䈭䉎ઁ䈱⢻ജ䈮䈧䈇䈩
䉅⹏ଔ䈚䈩䉂䈢䈇


2

ቇ䉖䈣䈖䈫䈲
⾗ᩰขᓧ䈮ᓎ┙䈧䈫ᕁ䈉ቴⷰ⊛䈮⷗⋥䈜䈖䈫䈏䈪䈐䈢

2

ʻࢸƷᛢ᫆•ᧄ⎇ⓥ䈪䈲䋬䉮䊮䊏䊁䊮䉲䊘䊷䊃䊐䉤䊥䉥䈱ឭ␜ᣇᴺ䈱᦭ലᕈ䉇㪃䉮䊮䊏䊁䊮䉲䊘䊷䊃䊐䉤䊥䉥䈱⸳⸘⹏ଔ䈮㑐䈜䉎᦭ലᕈ䉁䈪䈱ᬌ⸽䈲㪃ᚑ䈘䉏䈩䈇䈭䈇䋮੹ᓟ䈲䈖䈉䈚䈢ὐ䈮䈧䈇䈩ᦝ䈮⎇ⓥ䉕ㅴ䉄䈩䈇䈐䈢䈇䋮－ 10 －⥃ ᐥࠕࡠ ࡑ࠮࡜ ࡇ࡯ߩ −∩೙ ᓮߩ␹ ⚻↢ℂ ቇ⊛ᬌ ⸛ဌ၂෹⟤ 1,᩿␽㓷 2㧘Ꮉේ㕏ᒄ 3㧘ቝ૒⟤⋿ 4㪈ᗲ⍮ක⑼ᄢቇ㩷 ⋴⼔ቇㇱ㩷 ᗲ⍮⋵ᗲ⍮㇭㐳ਭᚻ↸ᄢሼጤ૞ሼ㓵෶ 㪉㪈㩷⁛㪀㩷 ᖱႎㅢା⎇ⓥᯏ᭴⣖ᖱႎㅢାⲢว⎇ⓥᚲ䋬㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷㪊᡼ㅍᄢቇ㩷 ᢎ㙃ቇㇱ䋬㩷 㪋 ␹ᚭᄢቇᄢቇ㒮㩷 ଻ஜቇ⎇ⓥ⑼㩷
㪉

email: bohgaki@aichi-med-u.ac.jp
Keywords: ࠕࡠࡑ࠮࡜ࡇ࡯㧘༦ⷡ㧘⣖ᵄ㧘␹⚻↢ℂ∛ᘒߩㅴⴕߣߣ߽ߦ␹⚻ࠍ฽߻᭽‫߇❱⚵ߥޘ‬ଚⷅߐࠇ⊒∝ߔࠆ≸−∩ࠍ೙ᓮߔࠆߎߣ
ߪ‫ߩߩ߽ߩߘ≸ޔ‬ᴦ≮ߣหᤨߦᰳ߆ߖߥ޿ක≮ߢ޽ࠆ‫≸ޕ‬−∩㒰∩ߢߪ‫ޔ‬ㅴⴕᐲߦᔕߓࠝ
ࡇࠝࠗ࠼♽㎾∩⮎߇ᛩਈߐࠇ㜞ᄼല₸ࠍᓧߡ޿ࠆ‫❱⚵♽⚻␹ߒ߆ߒޕ‬ଚⷅߩ႐วߦߪࠬ࠹
ࡠࠗ࠼߿᛫߁ߟ⮎ߒ߆᦭ലߥᓢ∩⮎ߪߥߊ‫‛⮎ޔ‬⠴ᕈ‫ޔ‬ᒝ޿೽૞↪߇ᴦ≮਄ߩᄢ߈ߥ໧㗴ߣߥߞߡ޿ߚ‫ޕ‬೽૞↪ߩ࡝ࠬࠢ߇ᦨዊ㒢ߢ޽ࠆࠕࡠࡑ࠮࡜ࡇ࡯ߪ‫⚻␹ޔ‬ᕈ≸−∩㒰∩ߩ᦭ജߥ୥⵬ߢ޽ࠅ‫ޔ‬ઍᦧක≮ߣߒߡߔߢߦታᣉߐࠇߡ޿ࠆ‫ޔߒ߆ߒޕ‬ㅢᏱක≮ߩ▸࿐ߢㆡ↪
ߔࠆߚ߼ߦߪ⑼ቇ⊛ᩮ᜚ߦၮߠߊ૞↪ᯏᐨߩ᣿⏕ൻ߇ᔅ㗇ߢ޽ࠆ‫ޕ‬ᚒ‫ޔߪޘ‬⣖ᵄ⸘᷹ࠍਛ
ᔃߦหᤨ⸘᷹ߔࠆ⒳‫↢ߩޘ‬ℂᜰᮡ߆ࠄ␹⚻↢ℂቇ⊛ߥࠕࡠࡑߩ㒰∩ࡔࠞ࠾࠭ࡓߩ⸃᣿ࠍ
⋡ᜰߒߡ޿ࠆ‫੹ޕ‬࿁‫ޔ‬ஜᏱ⠪ࠍኻ⽎ߦ‫ࡑࡠࠕߥ⊛ဳౖޔ‬ಽሶࠍ༦ⷡ࡮⊹⤏߆ࠄੱ૕ߦ૞↪
ߐߖߡࠕࡠࡑߩၮᧄ⊛േᘒߦ㑐ߔࠆࡄࠗࡠ࠶࠻⹜㛎ࠍⴕߞߚ‫ޕ‬ ઍஜᏱ↵ᕈߦኻߔࠆ⹜㛎ߢߪ‫ࡑࡠࠕޔ‬ๆ౉㐿ᆎᓟ߆ࠄ⊹⾰ో૕ߦᐢ߇ࠆǩᵄ߇᷹ⷰߐ
ࠇ㧔࿑㧝㧕‫ޔ‬ๆᒁ⚳ੌᓟ߽ᜬ⛯ߒߚ‫ߩߎޕ‬ǩᵄߦߪౖဳ⊛ߥYCZKPI  YCPKPI YY ߇޽ࠅ‫ో⾰⊹ߟ߆ޔ‬૕ߢᄌേ๟ᦼ߇หᦼߒߡ޿ߚ‫ߥ߁ࠃߩߎޕ‬േᘒߪ㎾㕒૞↪߿ⷡ㉕૞↪ࠍᜬߟࠝࠗ࡞ߩ⒳㘃ߦଐሽߖߕ಴⃻ߔࠆ௑ะߦ޽ߞߚ‫ⷰޕ‬᷹ߐࠇߚYYߪਛ⣖㤥⾰ㇱߩࡕࡁࠕࡒࡦ␹⚻ᵴേߣᱜߩ⋧㑐ࠍᜬߟߣߐࠇߡ߅ࠅ‫߇ࡑࡠࠕޔ‬⣖ో૕ࠍᵴᕈൻߒᓧࠆߎߣ߇␜ໂ
ߐࠇࠆ‫৻ޕ‬ᣇ‫ޔ‬๭ๆߣᔃᜉߪ‫ࡑࡠࠕޔ‬ๆᒁᤨߢߪ‫ޔ‬หᦼߔࠆ௑ะߦ޽ࠅ‫ߟ߆ޔ‬೽੤ᗵ␹⚻ᵴേߩᜰᮡߣߐࠇࠆ*(.(ߪᔅߕߒ߽ᄢ߈ߊߥ߆ߞߚ㧔࿑㧕‫߇ࡑࡠࠕߪࠇߎޕ‬೽੤ᗵ␹⚻♽ࠍ⾮ᵴߔࠆන⚐ߥ࡝࡜ࠢ࠯࡯࡚ࠪࡦലᨐࠍਈ߃ࠆ߽ߩߢߥߊ‫ޔ‬๭ๆ࡮ᓴⅣེ♽ࠍ฽߼ߡり૕ᵴേߩᦨㆡൻࠍⴕ߁૞↪ࠍ᦭ߔࠆ߽ߩߢ޽ࠆߎߣ߇␜ໂߐࠇࠆ‫ޕ‬ ੹ᓟ‫ࠆࠃߦࡑࡠࠕޔ‬㒰∩ߩ૞↪ᯏᐨࠍ㜞ᰴ⣖ᯏ⢻ߦ߅ߌࠆㆬᛯ⊛−∩ᛥ೙೙ᓮᯏ᭴ߣ⠨߃‫ޔ‬⣖ᯏ⢻⸘᷹ࠍਛᔃߣߒߚ⎇ⓥࠍㅴ߼ߡ޿ߊ‫ޕ‬ ᧄ⎇ⓥߩ৻ㇱߪ⑼ቇᛛⴚᝄ⥝ᯏ᭴,56%4'56ߩ⵬ഥࠍฃߌታᣉߒߚ‫ޕ‬

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࿑ 㪉㩷 ᔃᜉ䊶๭ๆ䈱䊥䉵䊛㩷࿑ 㪈㩷 䉝䊨䊙ๆᒁਛ䈱⣖ᵄ㩷

－ 11 －

ᄙᗵⷡ⊛ߥᄖㇱೝỗߩᄌൻߦኻߔࠆ
ࡅ࠻⣖ᵴേߩᬌ⸛㕍ጊᢕ, ᩙၔ⋿਽᧲੩㔚ᯏᄢቇ వ┵Ꮏቇ⎇ⓥᚲ‫ޔ‬ජ⪲⋵ශ⷏Ꮢᱞ⷏ቇ࿦บ 2-1200
email: aoyama@rcat.dendai.ac.jp
Keywords: ᄙᗵⷡᖱႎಣℂ㧘ᣧᦼ⣖ᵴേ㧘ⷞ⡬ⷡೝỗ㧘ㅪว⍮ⷡ㧘⣖⏛⇇⸘᷹ᴺ㧔MEG㧕ⷐᣦੱ㑆߇ᄙᗵⷡ⊛ߥᄖㇱⅣႺߦㆡᔕߔࠆߦ޽ߚߞߡ㧘ⅣႺ߆ࠄಽᢔ⊛ߦ౉ജߐࠇࠆᗵⷡᖱႎࠍㄦㅦߦㅪวߔࠆߎߣߪ㊀ⷐߢ޽ࠆ㧚ߒ߆ߒߥ߇ࠄ㧘ㅪว⍮ⷡߩᣧᦼ↢ᚑߩ⣖ౝᯏᐨߦߟ޿ߡߪਇ᣿ߥὐ߇ᄙ޿㧚ᧄ⎇ⓥߢߪ㧘ⷞ⡬ⷡೝỗߩਇ৻⥌㧔଀㧦#8#8#8#8#8̖㧕ߦኻߒߡ⊒↢ߔࠆᓸᒙߥ⣖⏛⇇㧔/')㧕ࠍ⸘᷹ߒ㧘ੱ㑆ߩ⣖ౝߦ߅ߌࠆᄙᗵⷡ⊛ߥㅪวಣℂߩታ㛎⊛ᬌ⸛ࠍⴕߞߚⷞⷡೝỗߣ⡬ⷡೝỗߦᤨ㑆Ꮕ߇޽ࠆ㓙㧘ਇ৻⥌๒␜ᓟ OU ⒟ᐲߢ⡬ⷡ㊁ࠍ฽߻਄஥㗡ㇱߣⷞⷡ㊁ࠍ฽߻ᬥㇱߩᵴേᒝᐲ߇
Ⴧᄢߒ㧘ߘߩᓟǩ㨪ǪᏪၞߩ⢛᥊⣖ᵴേ߇ᄌൻߒߚ㧚ߎߩߎߣ߆ࠄ㧘⡬ⷡ
㊁ߣⷞⷡ㊁ߪ᧪ࠆߴ߈ᖱႎࠍ੍᷹ߔࠆߎߣߢㅪว⍮ⷡࠍ OU ⒟ᐲߢᒻᚑ
ߔࠆߎߣ߇ߢ߈㧘ߘߩᓟߩ⣖ᵴേߪ⹺⍮⊛࡮ⴕേ⊛ߥ᳿ቯߣ㑐ㅪ߇޽ࠆߣ
⠨߃ࠄࠇߚ㧚੹ᓟߩዷᦸ‫ޟ‬ᄙᗵⷡ⊛ߥᖱႎߩㅪวಣℂߪ㧘㓏ጀ⊛ߦฦ‫ߩޘ‬ᗵⷡᖱႎಣℂߩ਄૏ߦ૏
⟎ઃߌࠄࠇࠆ߽ߩߢߪߥߊ㧘ૐᰴߩᗵⷡ㊁߆ࠄ⋧੕⊛ߦ㐿ᆎߔࠆ㧔⡬ⷡᖱ
ႎ߇ⷞⷡ㊁ߢ߽ಣℂߐࠇࠆ㧕‫߁޿ߣޠ‬઒⺑ࠍታ⸽ߔࠆߚ߼ߦ㧘ᧄታ㛎ߦ㑐ߒߡᦝߥࠆ⸃ᨆ߿ᬌ⸛㧔ᤨ㑆๟ᵄᢙ⸃ᨆ㧘ᵴേḮ⸃ᨆߣᵴേḮߩ࿃ᨐ㑐ଥߩᬌ⸛╬㧕ࠍㅴ߼ࠆ㧚߹ߚ೎ࡄ࡜࠳ࠗࡓߦ߅޿ߡᦝߥࠆታ㛎⊛⸽᜚ࠍ෼㓸ߔࠆ㧚－ 12 －

᧲ᣣᧄᄢ㔡ἴߢⵍἴߒߚሶߤ߽ߚߜߩ
ኋᴱဳࠠࡖࡦࡊߦ߅ߌࠆኅᣖߣߩᖱႎ੤឵
ʊ⺞ᩏᦠ࡮ႎ๔ᦠߩㆇ↪ࠍㅢߒߡʊ
⋧Ỉᒎ↢㧘ጟፒᕺ೑㧘Ꮉ⋡⵨㧘ṚỈ౏ሶ㧘ቶફ߈ߺሶ

߅⨥ߩ᳓ᅚሶᄢቇᄢቇ㒮 ੱ㑆ᢥൻഃᚑ⑼ቇ⎇ⓥ⑼᧲੩ㇺᢥ੩඙ᄢႦ 2-1-1
email: g1140560@edu.cc.ocha.ac.jp
Keywords: 㔡ἴ㧘ሶߤ߽㧘ኅᣖ㧘ࠠࡖࡦࡊⷐᣦ㔡ἴߢ଻⼔⠪ࠍᄬߞߚሶߤ߽ࠍᡰេߔࠆ‫ޟ‬ᄞߩߟ߫ߐࡊࡠࠫࠚࠢ࠻‫ߢޠ‬ߪ‫ޔ‬ሶߤ߽ߣቇ↢ࡏ࡜ࡦ࠹ࠖࠕߩኋᴱဳࠠࡖࡦࡊࠍታᣉߒ‫ߩߘޔ‬ਛߢኅᣖߣߩᖱႎ੤឵ߣߒߡ⺞ᩏᦠ‫ޔ‬ႎ๔ᦠࠍ૞ᚑߒߚ‫⺖ߩࠄࠇߘޕ‬㗴ߣ੹ᓟߩ⺖㗴ߦߟ޿ߡ⠨ኤߔࠆ‫ޕ‬

੹ᓟߩዷᦸ
 

੹ᓟߩࠠࡖࡦࡊߢ⺞ᩏᦠ‫ޔ‬ႎ๔ᦠߩ૶↪ࠍ⛮⛯ߒߡ޿ߊਛߢ‫ߏޔ‬ኅᣖߩ࠾࡯࠭ߦวߞߚ߽ߩߦߔࠆߚ߼ߦ‫ޔ‬ౝኈ߿⹏ଔߩᣇᴺߦߟ޿ߡߩᬌ⸛ࠍㅴ
߼ߡ޿߈ߚ޿‫ߚ߹ޕ‬ᄞߩߟ߫ߐࡊࡠࠫࠚࠢ࠻ߢߪ‫੹ޔ‬ᓟሶߤ߽ߚߜߩㅴቇ
߿ዞ⡯ߦߟ޿ߡߩ⋧⺣ᬺോ߽ⴕߞߡ޿ߊ੍ቯߢ޽ࠆ‫ߩߘޕ‬㓙ߦ‫ޔ‬ሶߤ߽ߚߜߩ᭽ሶࠍ⸥㍳ߒߚࠠࡖࡦࡊߢߩႎ๔ᦠࠍߤߩࠃ߁ߦ↢߆ߒߡ޿ߊߎߣ߇
ߢ߈ࠆ߆⠨߃ߡ޿ߊᔅⷐ߇޽ࠆ‫ޕ‬－ 13 －

ᮾ᪥ᮏ኱㟈⅏䛷⿕⅏䛧䛯Ꮚ䛹䜒䛯䛱䛾ᐟἩᆺ䜻䝱䞁䝥䛻䛚䛡䜛ᐙ᪘䛸䛾᝟ሗ஺᥮―ㄪᰝ᭩䞉ሗ࿌᭩䛾㐠⏝䜢㏻䛧䛶―䕿┦⃝ᘺ⏕䚸ᒸᓮᜨ฼䚸ᕝ┠⿱䚸⁪⃝බᏊ䚸ᐊఅ䛝䜏Ꮚ䛚Ⲕ䛾ỈዪᏊ኱Ꮫ኱Ꮫ㝔㻌 ே㛫ᩥ໬๰ᡂ⛉Ꮫ◊✲⛉䛿䛨䜑䛻㻌 2011ᖺ3᭶11᪥䛻䚸୕㝣Ἀ䜢㟈※ᆅ䛸䛧䛯䝬䜾䝙䝏䝳䞊䝗 9.0 䛾ᕧ኱ᆅ㟈䛜Ⓨ⏕䛧䚸ኴᖹὒἢᓊ䜢୰ᚰ䛻ᕧ኱䛺ὠἼ䜢ㄏⓎ䛧䛶䚸ከ䛟䛾᪉䚻䛾㈗㔜䛺
⏕࿨䜢ዣ䛳䛯䚹ክ䛾䛴䜀䛥䝥䝻䝆䜵䜽䝖䛿䚸ಖㆤ⪅䜢ஸ䛟䛥䜜䛯Ꮚ䛹䜒䛯䛱䛜୍⥴䛻ᴦ䛧䛔᫬䜢㐣䛤䛩ሙ䜢ᐃᮇⓗ䛻タ䛡䛶䚸䛭䛾ᡂ㛗䜢㛗䛟ぢᏲ䜛άື䛷䛒䜛䚹2011ᖺ8᭶䛻䛿䝖䝷䜲䜰䝹䜻䝱䞁䝥䜢ᐇ᪋䛧䚸䝇䝫䞊䝒䜔䝺䜽䝸䜶䞊䝅䝵䞁䚸䝁䞁䝃䞊䝖䚸ᕤሙぢᏛ䛺䛹䛾䛥䜎䛦䜎䛺άື䜢⾜䛳䛯䚹㻌 ௒ᅇ䛾䜻䝱䞁䝥䛷䚸஦๓䛻Ꮚ䛹䜒䛯䛱䛾᝟ሗ䜢ㄪᰝ᭩䛻グධ䛧䛶䛔䛯䛰䛝䚸䜻䝱䞁䝥୰䛾ᵝᏊ䜢ሗ࿌᭩䛸䛧䛶䛤ᐙ᪘䛻䛚Ώ䛧䛩䜛ヨ䜏䜢⾜䛳䛯䚹㐠⏝᪉ἲ䛸௒ᚋ䛾ㄢ㢟䛻䛴䛔䛶ሗ࿌䛩䜛䚹䛺䛬ㄪᰝ᭩䞉ሗ࿌᭩䜢సᡂ䛧䜘䛖䛸⪃䛘䛯䛾䛛⚾䛯䛱䛾㢪䛔
䜻䝱䞁䝥䛷ᴦ䛧䛟Ᏻ඲䛻
䛻
㐣䛤䛧䛶䜒
䜒䜙䛔䛯䛔

Ꮚ䛹䜒䛯䛱䛻䛻ඖẼ䛻䛻䛺䛳䛶䜒䜙䛔䛯䛔䜻䝱䞁䝥䜢⾜䛖ୖ䛷䚸Ᏻ඲䜢☜ಖ䛧Ꮚ䛹䜒䛯䛱䛻ᴦ䛧䜣䛷䜒䜙䛖䛯䜑䛻䚸Ꮚ䛹䜒䛯䛱䛾ಶูᛶ䜢㓄៖䛧䛯άື䜢⾜䛖䛣䛸䛜㔜せ䛷䛒䜛䚹஦๓䛻Ꮚ䛹䜒䛯䛱䛾᝟ሗ䜢䛤ᐙ᪘䛻ᥦ౪䛧䛶䛔䛯䛰䛝䚸ཧ⪃䛻䛧䛯䛔䚹

ᐙ᪘䛜Ᏻᚰ䛧䛶ᏊᏊ䛹䜒䜢䜢
㏦䜚ฟ䛫䜛䜻䝱䞁䝥䛻䛻䛧䛯䛔z ௒ᅇ䛾䜻䝱䞁䝥䛾ཧຍ⪅䛿⿕⅏䛧䛯Ꮚ䛹䜒䚸䛚䜘䜃 z䛭䜜䛻䜘䜚ಖㆤ⪅䜢ኻ䛳䛯Ꮚ䛹䜒䛷䛒䜛䚹⮬↛⅏ᐖ䛺䛹䛾እയⓗฟ᮶஦䛿䝇䝖䝺䝇※䛸䛺䜚䚸ᚰⓗእയᚋ䝇䝖䝺䝇㞀ᐖ䜢Ⓨ⑕䛩䜛䛣䛸䛜䛒䜛䛯䜑䚸ᚰ⌮ⓗᨭ᥼䛜ᚲせ䛷䛒䜛䚹z Ꮫ⏕䝪䝷䞁䝔䜱䜰䛻䛸䛳䛶䚸ึ䜑䛶఍䛳䛯Ꮚ䛹䜒䛸ᩘ᪥㛫㐣䛤䛧䚸ୡヰ䜢䛩䜛ᶵ఍䛿ᑡ䛺䛔䛯䜑䚸Ꮚ䛹䜒䛾 zᵝᏊ䛻Ẽ䜢㓄䜜䜛䜘䛖䛺ᣦᑟ䛜ᚲせ䛸䛺䜛䚹ሗ࿌᭩䛻Ꮚ䛹䜒䛯䛱䛾ᵝᏊ䜢グධ䛩䜛䛣䛸䛷䚸Ꮚ䛹䜒䛯䛱䛾ᑠ䛥䛺ኚ໬䜢䜘䜚ὀព῝䛟ほᐹ䛧䛶䛔䛝䛯䛔䚹ㄪᰝ᭩䛷䛤ᐙ᪘䛛䜙㡬䛔䛯᝟ሗ䛻ᇶ䛵䛝ሗ࿌᭩䜢సᡂ䛧䛶䚸䝣䜱䞊䝗䝞䝑䜽䛧䛯䛔䚹ሗ࿌᭩䛾సᡂᣦ㔪ㄪᰝ᭩䛾సᡂᣦ㔪z ཧຍ䛩䜛Ꮚ䛹䜒䛾೺ᗣ≧ែ䛻㛵䛩䜛᝟ሗ䜢஦๓䛻ᢕᥱ䛧䚸㐺ษ䛺ᑐᛂ䚸⥭ᛴ᫬䛾㎿㏿䛺ᑐᛂ䛜䛷䛝䜛䜘䛖‽ഛ䛩䜛䚹z ᐙ᪘䛜ឤ䛨䛶䛔䜛ᚰ㓄䜔୙Ᏻ䜢஦๓䛻☜ㄆ䛧䚸Ꮚ䛹䜒䛸᥋䛩䜛㝿䛻ᙺ❧䛶䜛䚹z 䜻䝱䞁䝥୰䚸Ꮚ䛹䜒䛜䛹䛾䜘䛖䛺άື䜢䛧䛯䛾䛛䚸䛹䛾 z Ꮫ⏕䝪䝷䞁䝔䜱䜰䚸┳ㆤᖌ䛸䛔䛖䠎䛴䛾ഃ㠃䛛䜙䛾᝟ሗ䜘䛖䛺ᵝᏊ䛰䛳䛯䛾䛛䛻䛴䛔䛶グ㏙䛩䜛䚹䜢ᥦ౪䛩䜛䛣䛸䛷䚸Ꮚ䛹䜒䛾ᵝᏊ䜢䜘䜚ලయⓗ䛻ఏ䛘䜛䚹z 䜻䝱䞁䝥୰䛾Ꮚ䛹䜒䛾೺ᗣ㠃䛷䛾ኚ໬䜔㣗஦䛾ᦤྲྀ z Ꮫ⏕䝪䝷䞁䝔䜱䜰䛜Ꮚ䛹䜒䛾ᵝᏊ䜢ほᐹ䛧䚸ᑠ䛥䛺ኚ໬≧ἣ䛻䛴䛔䛶グ㏙䛩䜛䚹䛻䜒Ẽ䛵䛡䜛䜘䛖䛻䛩䜛䚹㐠⏝᪉ἲᐇ㝿䛾⏝⣬䛸グධ౛䜻䝱䞁䝥๓ㄪᰝ᭩䠄⾲䠅

ᐙ᪘䛻ㄪᰝ᭩䛾グධ䜢౫㢗䚸
㒑㏦䛧䛶䛔䛯䛰䛟

Ꮚ䛹䜒䛯䛱䛻䛸䛳䛶䚸㟈⅏ᚋึ䜑䛶ᐙ᪘䛾チ䜢㞳䜜䛶㐣䛤䛩ᶵ఍䛷䛒䜛䛣䛸䛜⪃䛘䜙䜜䚸㏦䜚ฟ䛩ᐙ᪘䛿ᖹᖖ᫬䛸ẚ䜉୙Ᏻ䛜ቑຍ䛧䛶䛔䜛䛣䛸䛜ண᝿䛥䜜䜛䚹䜎䛯ᐙ᪘䜒⿕⅏⪅䛷䛒䜚ᚰⓗእയ䜢ཷ䛡䛶䛔䜛䛯䜑䚸௒ᅇ䛾䜻䝱䞁䝥䜈䛾ཧຍ䛷᪂䛯䛺୙Ᏻ䛜⏕䜎䜜䛺䛔䜘䛖㓄៖䛩䜛ᚲせ䛜䛒䜛䚹䜻䝱䞁䝥䛷᪥ᖖ䛸䛿␗䛺䜛⤒㦂䜢䛩䜛䛣䛸䛿䚸Ꮚ䛹䜒䛯䛱䛜ᡂ㛗䛩䜛䜘䛔ᶵ఍䛸䛺䜛䛯䜑䚸䛭䛣䛷䛾Ꮚ䛹䜒䛯䛱䛾ᵝᏊ䜢▱䜙䛫䜛䛣䛸䛿䚸ᐙ᪘䛻䛸䛳䛶䜒႐䜃䛸⪃䛘䜙䜜䜛䚹ሗ࿌᭩ㄪᰝ᭩䠄⿬䠅

䝇䝍䝑䝣㛫䛷᝟ሗ䜢ඹ᭷䜻䝱䞁䝥୰䛭䛾᪥䛾⤊஢ᚋ䚸Ꮫ⏕䝪䝷㻌䞁䝔䜱䜰䛜άືෆᐜ䚸Ꮚ䛹䜒䛾ᵝᏊ䚸㣗஦ෆᐜ䚸㣗஦䛾᫬䛾ᵝᏊ䜢ሗ࿌᭩䛻グ㍕⤊஢᪥䛻䚸䜻䝱䞁䝥୰䛾೺ᗣ≧ែ䛻䛴䛔䛶䛾᝟ሗ䜢┳ㆤᖌ䛜䜎䛸䜑䚸ሗ࿌᭩䛻グ㍕

䜻䝱䞁䝥ᚋ䜻䝱䞁䝥䝇䝍䝑䝣䜘䜚䛤ᐙ᪘䛻᏶ᡂ䛧䛯ሗ࿌᭩䜢Ώ䛩*Ꮚ䛹䜒䛜ཧຍ䛩䜛䜻䝱䞁䝥䚸㧗㱋⪅䛾௓ㆤண㜵㏻ᡤ௓ㆤ䝃䞊䝡䝇䠄䝕䜲䝃䞊䝡䝇䠅䛺䛹䛷ㄪᰝ᭩䞉ሗ࿌᭩䜢
㏻䛧䛯᝟ሗ஺᥮䛜䛩䛷䛻⾜䜟䜜䛶䛔䜛䛯䜑䚸䛭䜜䜙䜢ཧ⪃䛻⏝⣬䜢సᡂ䛧䛯䚹

ㅰ㎡

⪃ᐹ䛸ㄢ㢟
㻌 ௒ᅇ䛿䝖䝷䜲䜰䝹䜻䝱䞁䝥䛾䛯䜑ཧຍ⪅䛜ᑡேᩘ䛷䛒䜚䚸┠䛜ᒆ䛝䜔䛩䛔≧ἣ䛷䛒䛳䛯䛜䚸௒ᚋཧຍ⪅䛾ቑຍ䛻క䛔ㄪᰝ᭩䛾ෆᐜ䛻㛵䛧䛶䝇䝍䝑䝣㛫䛷䛾᝟ሗඹ᭷䜢ᚭᗏ䛧䛶䛔䛟ᚲせ䛜䛒䜛䚹㻌 䜎䛯ཧຍ⪅䛜ቑ䛘䛶䜒䚸ሗ࿌᭩䜢䛤ᐙ᪘䛻႐䜣䛷䛔䛯䛰䛡䜛ෆᐜ䛻䛩䜛䛯䜑䛻䚸Ꮫ⏕䝪䝷䞁䝔䜱䜰䛜ᑠ䛥䛺ኚ໬䛻䜒Ẽ䛵䛡䜛䜘䛖䛺యไ䜢⥔ᣢ䛧䛶䛔䛛䛺䛡䜜䜀䛺䜙䛺䛔䚹Ꮚ䛹䜒䛯䛱䜈䛾䛝䜑⣽䛛䛔㓄៖䜢⥅⥆䛧䛶䛔䛟䛯䜑䛻䚸௒ᚋ䛾䜻䝱䞁䝥䛷䜒Ꮚ䛹䜒䠎ே䛻ᑐ䛧Ꮫ⏕䝪䝷䞁䝔䜱䜰䠏䡚䠐ே䛜ୡヰ䜢䛩䜛యไ䜢䛸䜛ணᐃ䛷䛒䜛䚹㻌 ᚋ᪥䚸䛤ᐙ᪘䛻Ꮚ䛹䜒䛯䛱䛾ᵝᏊ䜢ఛ䛳䛯䛸䛣䜝䚸䛂Ꮚ䛹䜒䛯䛱䛜ᴦ䛧䛭䛖䛻䜻䝱䞁䝥䛾䛣䛸䜢ヰ䛧䛶䛟䜜䛯䛃䚸䛂⤮᪥グ䛻᭩䛔䛶Ꮫᰯ䛷ඛ⏕䜔཭㐩䛻ヰ䛧䜢䛧䛯䛃䚸䛂䜎䛯⾜䛝䛯䛔䛸ゝ䛳䛶䛔䜛䛃䛺䛹䛸႐䜣䛷㡬䛟䛣䛸䛜䛷䛝䚸Ꮚ䛹䜒䛯䛱䛾೺ᗣ≧ែ䛾⟶⌮䛻㛵䛧䛶ྰᐃⓗ䛺ពぢ䛿≉䛻⪺䛛䜜䛺䛛䛳䛯䚹䜎䛯Ꮫ⏕䛛䜙䜒䚸䛂Ꮚ䛹䜒䛯䛱䛾ୡヰ䛾㛫䛻᭩䛟䛾䛿኱ኚ䛰䛳䛯䛜䚸䛤ᐙ᪘䜈䛾ሗ࿌䛿㔜せ䛺䛣䛸䛰䛸ᛮ䛖䛃䚸䛂⏕ά㠃䛾᝟ሗ䜢ຍ䛘䛯᪉䛜䜘䛔䛾䛷䛿䛺䛔䛛䛃䛺䛹䛾๓ྥ䛝䛺ពぢ䛜ከ䛟⪺䛛䜜䛯䚹䛣䜜䜙䛾䛣䛸䛛䜙䚸௒ᚋ䜒ᨵၿ䛿ᚲせ䛷䛒䜛䛜䚸┠ᶆ䛿䛚䛚䜐䛽㐩ᡂ䛥䜜䛯䛾䛷䛿䛺䛔䛛䛸⪃䛘䜛䚹㻌 ክ䛾䛴䜀䛥䝥䝻䝆䜵䜽䝖䛷䛿䚸䜻䝱䞁䝥άື䛾䜋䛛䛻䚸ᖖ᫬㐃⤡䜔┦ㄯ䜢ཷ䛡௜䛡䜛ሙ䜢タ䛡䚸་ᖌ䞉ᚰ⌮䜹䜴䞁䝉䝷䞊䞉㑇ఏ䜹䜴䞁䝉䝷䞊䚸ᩍᖌ➼䛾ᑓ㛛ᐙ䜙䛻䜘䜛┦ㄯᴗົ䜔㐍Ꮫ䜔ᑵ⫋䛺䛹䛾㐍㊰┦ㄯ䜢ィ⏬䛧䛶䛔䜛䚹䛭䛾䜘䛖䛺᫬䛻䜒䚸Ꮚ䛹䜒䛯䛱䛾ᵝᏊ䜢グ㘓䛧䛯ሗ࿌᭩䛿᭷⏝䛷䛒䜛䛸⪃䛘䜛䚹－ 14 －

ㄪᰝ᭩సᡂ䛻䛒䛯䜚༠ຊ䛧䛶ୗ䛥䛳䛯䚸䛚Ⲕ䛾ỈዪᏊ኱Ꮫே㛫ᩥ໬๰ᡂ⛉Ꮫ◊✲⛉䛾㜿㒊Ṍ⨾䛥䜣䚸≟ሯ┿⏤Ꮚ䛥䜣ㄪᰝ᭩䞉ሗ࿌᭩䛾㐠⏝䛻༠ຊ䛧䛶ୗ䛥䛳䛯䚸ክ䛾䛴䜀䛥䝥䝻䝆䜵䜽䝖䛾䝇䝍䝑䝣䛾ⓙᵝ䝕䜲䝃䞊䝡䝇䛷ᐇ㝿䛻౑⏝䛧䛶䛔䜛ሗ࿌᭩䜢ᥦ౪䛧䛶ୗ䛥䛳䛯䚸䜰䝃䝠䝃䞁䜽䝸䞊䞁ᰴᘧ఍♫㔝᪉䝕䜲䝃䞊䝡䝇䛾ⓙᵝ䛻ឤㅰ⏦䛧ୖ䛢䜎䛩ஜᏱ⠪ࠍኻ⽎ߣߒߚࡊࡠ࠰࠺ࠖ㓚ኂ⊒↢⺖㗴ታ㛎ߣ⣖ᵄ⸘᷹੹੗⛗⟤ሶ 1,1
2

᩿␽㓷 2, 㑐໪ሶ 1, Ꮉ෶ᢅ↵ 1␹ᚭᄢቇᄢቇ㒮 ଻ஜቇ⎇ⓥ⑼㧘౓ᐶ⋵␹ᚭᏒ㗇⏴඙෹߇ਐ 7-10-2⁛)ᖱႎㅢା⎇ⓥᯏ᭴ᧂ᧪ ICT ⎇ⓥᚲ㧘౓ᐶ⋵␹ᚭᏒ⷏඙ጤጟ↸ጤጟ 588-2
email: emikoimai@stu.kobe-u.ac.jp
Keywords: ࡊࡠ࠰࠺ࠖ㧘⸒⺆↥↢㧘ၮᐙ⣖㧘㖸㗀ಽᨆ㧘⣖ᵄⷐᣦઁ⠪ߣߩᖱႎ‫ޔ‬ᗧᕁવ㆐‫౒ޔ‬ᗵߦᔅⷐߥ㖸ჿࠦࡒࡘ࠾ࠤ࡯࡚ࠪࡦߦߪ⸒⪲ߩᛥ឴߿㑆ว޿ߣ޿ߞߚࡊࡠ࠰࠺ࠖ㧔㖿ᓞ㧕߇ਇนᰳߢ޽ࠆ‫↢↥ࠖ࠺࠰ࡠࡊޕ‬㓚ኂ㧔Prosody
Production Disorder㧧એ㒠 PPD㧕ߪ⣖៊்ᖚ⠪ߦߒ߫ߒ߫⷗ࠄࠇ‫ޔ‬㓚ኂߩ⊒↢ᯏᐨߦߟ޿ߡߪᗵᖱᯏ⢻ߦ⌕⋡ߒߚ⊹⾰ਛᔃߩ⼏⺰߇ਥᵹߢ޽ߞߚ‫⸥ޔߒ߆ߒޕ‬ᙘ‫ޔ‬ᗧ๧ᬌ⚝‫ޔ‬
㖸㖿ಣℂ‫ޔ‬ㆇേડ↹࡮ታⴕ‫ᧃޔ‬᪳ᗵⷡᖱႎ⛔ว╬ߩ৻ㅪᵴേߩ⚿ᨐߢ޽ࠆ⸒⺆↥↢ߩ␹⚻ၮ⋚ࠍ⊹⾰ߩߺߩᯏ⢻ߢ⺑᣿ߔࠆߎߣߦߪ㒢⇇߇↢ߓࠆ‫ޕ‬ᚒ‫⾰⊹ߣ⾰⊹ޔߪޘ‬ਅ‫ޔ‬⣖ᐙߩᯏ⢻ࠍ޽ࠊߖߚ㖸ჿ⸒⺆↥↢࠾ࡘ࡯࡜࡞ࡀ࠶࠻ࡢ࡯ࠢࡕ࠺࡞ࠍ⠨᩺ߒߚ‫ߩߎޕ‬ࡀ࠶࠻ࡢ࡯ࠢߩ㚟േߦߪࡕࡁࠕࡒࡦ␹⚻♽‫ࠅ߹ߟޔ‬ၮᐙ⣖ᯏ⢻㧔Fundamental Brain
Activity㧧FBA㧕ߩᵴᕈൻ߇ਇนᰳߢ޽ࠆߣ઒⺑ࠍ┙ߡߚ‫ޕ‬઒⺑ߩᬌ⸽ߦߪ PPD ߣ FBA
ᵴᕈᐲߣߩ⋧㑐ᕈࠍ␜ߔᔅⷐ߇޽ࠆߚ߼‫ߕ߹ޔ‬ஜᏱ⠪ߦࠬ࠻࡟ࠬ⽶⩄⸒⺆↥↢⺖㗴ࠍ⺖ߒߚᤨߩ⣖ᵄ᷹ቯ߅ࠃ߮⊒⹤ࠨࡦࡊ࡞ࠍ㖸㗀ಽᨆߔࠆ੍஻ታ㛎ࠍⴕߞߚߩߢႎ๔ߔ
ࠆ‫ޕ‬ታ㛎ߪஜᏱᚑੱ 2 ฬ㧔↵ᅚฦ 1 ฬ㧕ࠍኻ⽎ߣߒߡ⸒⺆↥↢⺖㗴㧔1.േ⹖ฏㆬᛯ㖸⺒㧘
2.ฬ⹖ᓳ໒㧘3.േ⹖ᗐ⿠⊒⺆㧘4.ᢥ㖸⺒㧕ࠍታᣉߒߚ‫ޕ‬㖸㗀ಽᨆࠃࠅ‫⥄ߪࠖ࠺࠰ࡠࡊޔ‬േ⊛⊒⺆⺖㗴㧔2,4㧕ߢߪ଻ߚࠇࠆ߽ߩߩ‫⺖ޔ‬㗴 1,3 ߢߪ㗼⪺ߦ㓚ኂߐࠇࠆߎߣ߇ࠊ߆
ߞߚ㧔࿑㧕‫ޕ‬⣖ᵄ᷹ቯࠃࠅ‫ޔ‬1,3 ߢߪ 2,4 ࠃࠅ߽ᐢ▸ߥ㗔㊁ߩ⣖ᵴേ߇⏕⹺ߢ߈ߚ‫߹ޕ‬ߚ‫ޔ‬ᣇ⇛┙ߡ㧔࿁╵ߩⷙೣൻ㧕߇࿎㔍ߥ 1 ߢߪᅚᕈࠃࠅ߽↵ᕈߢࡊࡠ࠰࠺ࠖ߇፣უߔ
ࠆ㗫ᐲ߇㜞߆ߞߚ‫ޕ‬એ਄ߩ⚿ᨐߪ‫ޔ‬⍍ᤨߩᗧ๧ᬌ⚝߿ᗧᕁ᳿ቯࠍᔅⷐߣߔࠆ⺖㗴㧔1,3㧕߇ஜᏱ⠪ߩ೨ㇱᏪ⁁࿁ߦㆊ೾ߥࠬ࠻࡟ࠬࠍ߆ߌ FBA ࠍ฽߻ࡕࡁࠕࡒࡦ␹⚻ᵴേࠍૐਅߐߖ‫ޔ‬೨㗡೨㊁ߢᗧᔒ᳿ቯࠍߔࠆᅚᕈߦᲧߴᏪ⁁࿁ߢᗧᕁ᳿ቯࠍߔࠆ↵ᕈߩ߶߁߇
PPD ࠍ⺃⊒ߒ߿ߔ޿ߎߣࠍ␜ໂߔࠆ‫ޕ‬ᄢ⣖⊹⾰៊்ߪ FBA ߩᑄ↪ᕈ⪜❗ࠍଦߒ‫ߩߘޔ‬⚿ᨐ‫↵ޔ‬ᕈߩߺߥࠄߕᅚᕈߢ߽ FBA ߦᡰ߃ࠄࠇߚ೨㗡⪲ᯏ⢻߇ૐਅߒ PPD ࠍ⺃⊒ߔࠆߣ޿߁ផ⺰ߦᚒ‫ߪޘ‬㆐ߒߚ‫ޕ‬੹ᓟߩዷᦸ੹ᓟ‫ޔ‬PDD ߣ FBA ߣߩ⋧㑐ᕈߦߟ޿ߡ↵ᅚᲧセࠍ฽߼ߡᄙߊߩ⥃ᐥ଀ࠍᬌ⸽ߒߡ
޿ߊ৻ᣇ‫ޔ‬઒⺑ߩ⋥ធ⊛ᬌ⸽ߩߚ߼ࡐࠫ࠻ࡠࡦᢿጀ᠟௝ᴺࠍ↪޿ߚᄢ⣖↢ℂቇ⊛ᬌᩏ߽ⴕߞߡ޿߈ߚ޿‫ޔߚ߹ޕ‬PPD ᡷༀߦะߌ‫᦭ޔ‬ലߥ FBA ᵴᕈൻᚻᲑࠍ↪޿ߚ޽ࠄߚߥ
⸒⺆࡝ࡂࡆ࡝࠹࡯࡚ࠪࡦߩᣇᴺ⺰ࠍ⏕┙ߒߡ޿ߊ‫ޕ‬－ 15 －

(Hz)500

䂦㖸⺒ 䋯 䂓ㆬᛯ

420
340
260
180

a

ge

te

100

     ࿑㧦ࡕ࡯࡜ߏߣߩࡇ࠶࠴ᦛ✢Ყセ㧔3 ࡕ࡯࡜⺆㧔‫ޠߡߍ޽ޟ‬㧦ૐ㜞ૐ㧕㧕    ㆬᛯᤨ‫߇✢ᦛ࠴࠶ࡇޔ‬ᐔ᧼ൻߔࠆ

－ 16 － ࠞ࡞ࡃⅣ⁁ࡎࠬࡈࠔ࠴ࠫࡦ㉄శቇ⇣ᕈ૕ߩ↢ℂ૞↪ߩᬌ⸛

㊁㦮⛗⟤ 1, ᓟ⮮⌀㉿ 2, ၳ↰᥍⟤ 3, ⧎Ỉୃ๺ 4, 5, ᫪ኡᢅ 6, ㋈ᧁቁᵗ 5,
ዊᨋㅴ 5, ቶફ߈ߺሶ 11߅⨥ߩ᳓ᅚሶᄢቇᄢቇ㒮 ੱ㑆ᢥൻഃᚑ⑼ቇ⎇ⓥ⑼᧲੩ㇺᢥ੩඙ᄢႦ 2-1-12߅⨥ߩ᳓ᅚሶᄢቇ ↢๮ᖱႎቇᢎ⢒࠮ࡦ࠲࡯3᧲੩ㇺஜᐽ㐳ኼක≮࠮ࡦ࠲࡯⎇ⓥᚲ᧲੩ㇺ᧼ᯅ඙ᩕ↸ 35-2
4߅⨥ߩ᳓ᅚሶᄢቇ ࠨࠗࠛࡦࠬ㧒ࠛ࠺ࡘࠤ࡯࡚ࠪࡦ࠮ࡦ࠲࡯
5
᧲੩ℂ⑼ᄢቇᄢቇ㒮 ⮎ቇ⎇ⓥ⑼㩷
ජ⪲⋵㊁↰Ꮢጊፒ 2641
6߅⨥ߩ᳓ᅚሶᄢቇ ߅⨥ᄢࠕࠞ࠺ࡒ࠶ࠢ࡮ࡊࡠ࠳࡚ࠢࠪࡦ
email:murofushi.kimiko@ocha.ac.jp  
Keywords: Ⅳ⁁ࡎࠬࡈࠔ࠴ࠫࡦ㉄‫ޔ‬శቇ⇣ᕈ૕‫ࡦࠪࠠ࠲࠻࡯ࠝޔ‬㒖ኂ‫ޔ‬     ߇ࠎ⚦⢩ᶐẢᛥ೙‫ߺ∩ޔ‬ᛥ೙ⷐᣦⅣ⁁ࡎࠬࡈࠔ࠴ࠫࡦ㉄㧔cPA㧕ߪ‫ࡦࠪࠠ࠲࠻࡯ࠝޔ‬㧔ATX㧕㒖ኂߦࠃߞߡ߇ࠎ⚦⢩ߩᶐẢ࡮ォ⒖ࠍᛥ೙‫߮ࠃ߅ޔ‬ή㜑␹⚻ C-fiber ࠍ੺ߒߡଚኂฃኈᕈ−∩ࠍᛥ೙ߔࠆ‫ޕ‬ᚒ‫↢ߩࠄࠇߎޔߪޘ‬ℂ૞↪ߦᔅ㗇ߩⅣ⁁࡝ࡦ㉄ၮࠍઍ⻢⊛ߦ቟ቯߐߖߚ⺃ዉ૕ߢ޽ࠆ 2 ࠞ࡞ࡃ cPA㧔2ccPA㧕ߩశቇ⇣ᕈ૕ࠍวᚑߒ‫↢ߩߘޔ‬ℂ૞↪ࠍᬌ⸛ߒߚ‫ޕ‬੹ᓟߩዷᦸᧄ⎇ⓥߦ߅޿ߡ‫( ޔ‬R)-2ccPA ߣ(S)-2ccPA ߩ㑆ߦߪ ATX 㒖ኂ‫⚦ࠎ߇ޔ‬⢩ᶐẢᛥ೙߅ࠃ߮∩ߺᛥ೙ലᨐߦ᦭ᗧߥᏅߪ⷗ࠄࠇߥ߆ߞߚ‫ޔࠄ߆ߣߎߩߎޕ‬2ccPA ߪวᚑߩ◲නߥ࡜࠮ࡒ૕ߢഃ⮎⎇ⓥ߳ᔕ↪࡮⊒ዷ߇น⢻ߢ޽ࠆߎߣ
߇␜ໂߐࠇߚ‫੹ޕ‬ᓟߪ‫ޔ‬2ccPA ߩ߽ߟ߇ࠎ⚦⢩ߩᶐẢᛥ೙߅ࠃ߮∩ߺᛥ೙ߩ⹦ߒ޿ࡔࠞ࠾࠭ࡓࠍ᣿ࠄ߆ߦߒ‫ޔ‬ഃ⮎⎇ⓥ߳ᔕ↪ߔࠆߎߣࠍ⋡ᜰߔ‫ޕ‬－ 17 －

⢛᥊㩷Ⅳ⁁ࡎࠬࡈࠔ࠴ࠫࡦ㉄㧔cPA㧕ߪ․ᓽ⊛ߥࠛࠬ࠹࡞⚿วߒߚⅣ⁁࡝ࡦ㉄ၮࠍ߽ߟ↢ℂᵴᕈ⢽⾰ߢ޽ࠆ 1)‫ޕ‬cPA ߪ‫ࡦࠪࠠ࠲࠻࡯ࠝޔ‬㧔ATX㧕ߩᵴᕈࠍ㒖ኂߒ‫⚦ޔ‬⢩ߩㆇേᯏ⢻ࠍมࠆ RhoA ߩᵴᕈൻࠍᛥ೙ߔࠆߎߣߢ‫⚦ࠎ߇ޔ‬⢩ߩᶐẢ࡮ォ⒖ࠍ
ᛥ೙ߔࠆߎߣ߇␜ໂߐࠇߡ߅ࠅ‫ ߦߢ߹ࠇߎޔ‬in vitro ߅ࠃ߮ in vivo ߢ cPA ߇߇ࠎ⚦⢩ߩᶐẢ࡮ォ⒖ࠍᛥ೙ߔࠆߎߣ߇ႎ๔ߐࠇߡ޿ࠆ 2), 3)‫ᦨߚ߹ޕ‬ㄭ‫ޔ‬cPA ߇ή㜑␹⚻✢⛽ߢ޽ࠆ C-fiber ࠍ੺ߒߚଚኂฃኈᕈ−∩ࠍᛥ೙ߔࠆߎߣ߽᣿ࠄ߆ߦߥߞ
ߚ 4)‫↢ߩࠄࠇߎޕ‬ℂ૞↪ߪ cPA ߇߽ߟⅣ⁁࡝ࡦ㉄ၮ߇㑐ਈߒߡ߅ࠅ‫ޔ‬ᚒ‫ޔߪޘ‬Ⅳ⁁࡝ࡦ㉄ၮࠍઍ⻢⊛ߦ቟ቯߐߖߚ⒳‫ ߩޘ‬cPA ⺃ዉ૕ࠍวᚑߒߡ߈ߚ‫ޕ‬⒳‫ ߩޘ‬cPA⺃ዉ૕ߩߥ߆ߢ sn-2 ૏ߩ࡝ࡦ㉄ၮࠍࡔ࠴࡟ࡦၮߦ⟎឵ߒߚ 2 ࠞ࡞ࡃⅣ⁁ࡎࠬࡈࠔ࠴ࠫࡦ㉄㧔2ccPA㧕߇ᦨ߽㗼⪺ߥ↢ℂᵴᕈࠍ߽ߟߎߣࠍ␜ߒߚ 3), 4)‫ޕ‬ߎࠇ߹ߢߦᚒ‫ ߚ޿ߡ޿↪߇ޘ‬2ccPA ߪ࡜࠮ࡒ૕ߢ޽ࠅ‫ޔ‬శቇ⚐☴ߢߪߥ߆ߞ
ߚ‫ߒ߆ߒޕ‬ഃ⮎ߩⷰὐ߆ࠄߪ‫ޔ‬2ccPA ߩశቇᵴᕈߩ㆑޿߇⒳‫↢ߩޘ‬ℂ૞↪߳ߤߩࠃ߁ߦᓇ㗀ߔࠆ߆ᬌ⸛ߔࠆᔅⷐ߇޽ࠆߣ⠨߃ࠄࠇߚ‫⎇ᧄޕ‬ⓥߢߪ‫ޔ‬ᣂߚߦวᚑ
ߐࠇߚ 2ccPA శቇ⇣ᕈ૕㧔R-2ccPA㧦࿑ 1‫ޔ‬S-2ccPA㧦࿑ 2㧕ߦࠃࠆ ATX 㒖ኂ‫ޔ‬߇ࠎ⚦⢩ߩᶐẢᛥ೙‫ޔ‬C-fiber ࠍ੺ߒߚଚኂฃኈᕈ−∩ߩᛥ೙ߦߟ޿ߡᬌ⸛ߒߚ‫ޕ‬







 
        ࿑ 1㧦R-2ccPA       ࿑ 2㧦S-2ccPA㩷㩷ᣇᴺ㩷ATX 㒖ኂ
ATX ߩ lysoPLD ᵴᕈߦࠃߞߡ࡝ࡦ㉄ၮ߇ട᳓ಽ⸃ߐࠇࠆߣⰯశࠍ⊒ߔࠆၮ⾰
FS-3 ࠍ↪޿ߡࡊ࡟࡯࠻ࠕ࠶࠮ࠗࠍⴕ޿‫ޔ‬2ccPA ߩ ATX 㒖ኂᵴᕈࠍ᷹ቯߒߚ‫ޕ‬ATX⛎ਈḮߣߒߡ‫⚦ࠎ߇੃࠻ࡅޔ‬⢩ MDA-MB-231 ߩၭ㙃਄ᷡ㧔CCM㧕ࠍ↪޿ߚ‫ޕ‬
0.01-10 ȝM ߩ(R), (S)-2ccPA ߅ࠃ߮࡜࠮ࡒ૕‫ޔ‬CCM‫ޔ‬FS-3 ࠍ 96 ࠙ࠚ࡞ࡊ࡟࡯࠻
ߦᷙวߒ‫ޔ‬37°C ߢ 2 ᤨ㑆෻ᔕߐߖߚ‫ߩߘޕ‬ᓟ‫Ⱟޔ‬శࡊ࡟࡯࠻࡝࡯࠳࡯ߢ FS-3 ߩ⊒ߔࠆⰯశᒝᐲࠍ᷹ቯߒ‫ޔ‬2ccPA శቇ⇣ᕈ૕߅ࠃ߮࡜࠮ࡒ૕ߩ ATX 㒖ኂᒝᐲࠍ᳞߼ߚ‫ޕ‬߇ࠎ⚦⢩ߩᶐẢᛥ೙ࡏࠗ࠺ࡦ࡮࠴ࡖࡦࡃ࡯ᴺࠍᔕ↪ߒ‫ޔ‬MDA-MB-231 ߩ߇ࠎ⚦⢩ᶐẢ⢻ࠍ᷹ቯߒߚ‫⋥ޕ‬ᓘ 8 ȝm ߩሹ߇޽޿ߚࡈࠖ࡞࠲࡯ߩ਄ㇱߦ Calcein-AM ߦࠃࠅⰯశᮡ⼂ߐࠇߚ MDA-MB-231 ࠍ᠞߈‫࡯࠲࡞ࠖࡈޔ‬ਅㇱߦ LPC ߣ(R), (S)-2ccPA ߹ߚߪ࡜࠮
ࡒ૕ࠍട߃ߚ‫ޕ‬37°C ߢ 6 ᤨ㑆ၭ㙃ߒߚᓟ‫࡯࠲࡞ࠖࡈޔ‬ਅㇱߦ⒖േߒߚ⚦⢩ߩⰯశᒝᐲࠍ᷹ቯߒ‫ޔ‬2ccPA శቇ⇣ᕈ૕߅ࠃ߮࡜࠮ࡒ૕ߩᶐẢᛥ೙ᒝᐲࠍ᳞߼ߚ‫ޕ‬C-fiber ࠍ੺ߒߚଚኂฃኈᕈ−∩ߩᛥ೙
㤗㈮࡜࠶࠻ࠍ↪޿ߡ⣄㜑૕ᕈ̆૕ᕈ෻኿ࠍ᷹ቯߒߚ‫ޕ‬㤗㈮ߒߚᓟ‫ߩ࠻࠶࡜ޔ‬᳇㆏‫✚ޔ‬㗖േ⣂ߦࠞ࠹࡯࠹࡞ࠍᝌ౉ߒ‫ੱࠇߙࠇߘޔ‬Ꮏ๭ๆེ‫ⴊޔ‬࿶⸘ߦធ⛯ߒߚ‫(ޕ‬R), (S)-2ccPA ߩ㕒⣂ౝᛩਈߩߚ߼ߦ‫ޔ‬㗪㕒⣂ߦࠞ࠹࡯࠹࡞ࠍᝌ౉ߒߚ‫ߘޕ‬－ 18 －

ߩᓟ‫ߩ࠻࠶࡜ޔ‬ฝᄢ⣽ㇱࠍಾ㐿ߒߡફ࿷␹⚻ߦ 10V ߩೝỗࠍਈ߃‫߇╭ޔ‬෼❗ߔࠆߣ߈ߩᵴേ㔚૏ߩᄌൻࠍ╭㔚⸘ߢ᷹ቯߒߚ‫ޕ‬ᓧࠄࠇߚ෻኿෻ᔕࠍᐔဋൻߒߚ
╭㔚࿑ߩᵄᒻ߆ࠄ㕙Ⓧࠍ᳞߼‫ޔ‬2ccPA శቇ⇣ᕈ૕ߩ෻኿ᛥ೙ᒝᐲࠍ᳞߼ߚ‫ޕ‬⚿ᨐ࡮⠨ኤ
ATX 㒖ኂ
2ccPA ߦࠃߞߡ ATX ߩ߽ߟ lysoPLD ᵴᕈ߇㒖ኂߐࠇ‫ޔ‬FS-3 ߩ⊒ߔࠆⰯశᒝᐲ
߇ 2ccPA ߩỚᐲଐሽ⊛ߦ㗼⪺ߦᷫዋߔࠆߎߣ߇␜ߐࠇߚ‫ߩߘޕ‬㒖ኂലᨐߦ‫ޔ‬శቇ⇣ᕈ૕ߦࠃࠆ᦭ᗧᏅߪ⷗ࠄࠇߥ߆ߞߚ㧔࿑ 3㧕‫ޕ‬












࿑ 3㧦2ccPA శቇ⇣ᕈ૕ߦࠃࠆ ATX 㒖ኂ߇ࠎ⚦⢩ߩᶐẢᛥ೙ࡈࠖ࡞࠲࡯ਅㇱߦ LPC ࠍട߃ߚᤨߣᲧߴߡ‫ޔ‬LPC ߣ౒ߦ 2ccPA ࠍട߃ࠆߣࡈ
ࠖ࡞࠲࡯਄ㇱ߆ࠄਅㇱ߳ᶐẢߔࠆ⚦⢩߇㗼⪺ߦᷫዋߔࠆߎߣ߇␜ߐࠇߚ‫ࠖࡈޕ‬࡞࠲࡯਄ㇱߦ᠞޿ߚ MDA-MB-231 ߇ಽᴲߒߚ ATX ߇ࡈࠖ࡞࠲࡯ਅㇱߦ᜛ᢔߒ‫ޔ‬ࡈࠖ࡞࠲࡯ਅㇱߩ LPC ߆ࠄ LPA ߇↢ᚑߐࠇ‫⚦ࠎ߇ޔ‬⢩߇ᶐẢߔࠆ߇‫ޔ‬LPC ߣ౒ߦ 2ccPA ࠍട߃ࠆߣ ATX ߩ௛߈߇㒖ኂߐࠇߚߚ߼ߛߣ⠨߃ࠄࠇࠆ‫ߩߘޕ‬ᛥ೙ലᨐߦ‫ޔ‬శቇ⇣ᕈ૕ߦࠃࠆ᦭ᗧᏅߪ⷗ࠄࠇߥ߆ߞߚ㧔࿑ 4㧕‫ޕ‬















࿑ 4㧦2ccPA శቇ⇣ᕈ૕ߦࠃࠆ߇ࠎ⚦⢩ᶐẢᛥ೙－ 19 －

C-fiber ࠍ੺ߒߚଚኂฃኈᕈ−∩ߩᛥ೙
㤗㈮ߒߚ࡜࠶࠻ߩᄢ⣽ㇱફ࿷␹⚻ߦ߅ߌࠆ⣄㜑૕ᕈ㧙૕ᕈ෻኿ߩ߁ߜ‫ޔ‬C-fiber ࠍ੺ߒߡ↢ߓࠆ C-reflex ߩߺࠍ 2ccPA ߇㗼⪺ߦᛥ೙ߔࠆߎߣ߇␜ߐࠇߚ‫ޕ‬ߘߩᛥ೙ലᨐߦ‫ޔ‬శቇ⇣ᕈ૕ߦࠃࠆ᦭ᗧᏅߪ⷗ࠄࠇߥ߆ߞߚ㧔࿑ 5㧕‫ޕ‬












࿑ 5㧦2ccPA శቇ⇣ᕈ૕ߦࠃࠆ C-fiber ࠍ੺ߒߚଚኂฃኈᕈ−∩ߩᛥ೙
2ccPA ߩ߽ߟ߇ࠎ⚦⢩ߩᶐẢᛥ೙߅ࠃ߮∩ߺᛥ೙ߩ⹦ߒ޿ࡔࠞ࠾࠭ࡓߪᧂߛ
ਇ᣿ߥߚ߼੹ᓟߐࠄߥࠆᬌ⸛߇ᔅⷐߢߪ޽ࠆ߇‫⎇ᧄޔ‬ⓥߩ⚿ᨐ߆ࠄ‫ޔ‬2ccPA ߪวᚑߩ◲නߥ࡜࠮ࡒ૕ߢഃ⮎⎇ⓥ߳ᔕ↪࡮⊒ዷ߇น⢻ߢ޽ࠆߎߣ߇␜ໂߐࠇߚ‫ޕ‬ᢥ₂㩷(1) K. Murakami-Murofushi, et al. J. Biol. Chem. 267 (1992) 21512-21517.
(2) D. L. Baker, et al. J. Biol. Chem. 281 (2006) 22786-22793.
(3) A. Uchiyama, et al. Biochim. Biophys. Acta 1771 (2007) 103-112.
(4) Y. Kakiuchi, et al. Mol. Pain 7 (2011)－ 20 －

േ‛⚦⢩ߩᾲࠬ࠻࡟ࠬᖱႎવ㆐ߦ㑐ਈߔࠆᣂⷙࠦ࡟ࠬ࠹࡝࡞ࠣ࡞ࠦࠪ࠼ %) วᚑ㉂⚛⑺ጊᄩሶ ૒‫ᧁޘ‬ᚑᳯ ự↰ീ ⧎Ỉୃ๺ ᓟ⮮⌀㉿ 㐳Ⴆ㕏ሶ ዊᨋㅴ ᐔᨋ⟵㓶 ቶફ߈ߺሶ ߅⨥ߩ᳓ᅚሶᄢቇᄢቇ㒮 ੱ㑆ᢥൻഃᚑ⑼ቇ⎇ⓥ⑼ ᧲੩ㇺᢥ੩඙ᄢႦ ർ㒽వ┵⑼ቇᛛⴚᄢቇ㒮ᄢቇ ࡑ࠹࡝ࠕ࡞ࠨࠗࠛࡦࠬ⎇ⓥ⑼
⍹Ꮉ⋵⢻⟤Ꮢᣩบ  ℂൻቇ⎇ⓥᚲ ⣖⑼ቇ✚ว⎇ⓥ࠮ࡦ࠲࡯ ၯ₹⋵๺శᏒᐢᴛ 

᧲੩ℂ⑼ᄢቇ ⮎ቇㇱ ↢๮ഃ⮎⑼ቇ⑼ ජ⪲⋵㊁↰Ꮢጊፒ 2641
email: akiyama.hisako2@ocha.ac.jp
Keywords: ࠦ࡟ࠬ࠹࡝࡞ࠣ࡞ࠦࠪ࠼ (CG)‫ޔ࡞࡯ࡠ࠹ࠬ࡟ࠦޔ‬ࠣ࡞ࠦࠪ࡞࠮࡜ࡒ࠼‫♧ޔ‬ォ⒖㉂⚛‫ޔ‬ᾲࠬ࠻࡟ࠬᖱႎવ㆐

ⷐᣦࠦ࡟ࠬ࠹࡝࡞ࠣ࡞ࠦࠪ࠼ %) ߪ‫ޔ‬േ‛⚦⢩ߩᾲࠬ࠻࡟ࠬᖱႎવ㆐ߩೋᦼㆊ⒟ࠍᜂ޿‫ޔ‬ᔕ╵ᕈࠍ೙ᓮߔࠆಽሶߣߒߡᚒ‫߇ޘ‬਎⇇ߦవ㚟ߌߡ⊒⷗ߒߚ♧⢽⾰ߢ޽ࠆ‫ޕ‬%) วᚑᯏ᭴ߪߎࠇ߹ߢᧂ⸃᣿ߢ޽ߞߚ߇‫੹ޔ‬࿁‫ޔ‬%) วᚑࠍᜂ߁ᣂߒ޿࠲ࠗࡊ
ߩ♧ォ⒖㉂⚛ࠍ⊒⷗ߒߚߩߢႎ๔ߔࠆ‫ޕ‬੹ᓟߩዷᦸ
ߎࠇ߹ߢߩ࿖ౝᄖߢߩᾲࠬ࠻࡟ࠬᖱႎવ㆐⎇ⓥߦ߅޿ߡ‫ߩߘޔ‬ೋᦼㆊ⒟ߦᔅ㗇ߥᾲࠬ࠻࡟ࠬ࠮ࡦࠨ࡯ߪᧂߛหቯߐࠇߡ޿ߥ޿‫੹ޕ‬࿁⑳ߚߜ߇⊒⷗ߒߚᣂߒ޿࠲ࠗࡊ
ߩ %) วᚑ㉂⚛ߪ‫ޔ‬ᾲࠬ࠻࡟ࠬ࠮ࡦࠨ࡯ߣߒߡᯏ⢻ߔࠆน⢻ᕈ߇㜞޿‫੹ޕ‬ᓟ‫ޔ‬㉂⚛ߩหቯ߇ㆀⴕߐࠇࠆߎߣߦࠃߞߡ‫⸃ᧂޔ‬᣿ߢ޽ߞߚᾲࠬ࠻࡟ࠬᖱႎવ㆐ߩೋᦼᗵ⍮ᯏ᭴߇᣿ࠄ߆ߦߥࠆߎߣ߇ᦼᓙߐࠇ‫ޔ‬േ‛⚦⢩ߩᕡᏱᕈ⛽ᜬᯏ᭴ߩ⸃᣿߳ߩ⓭⎕ญ߇㐿߆ࠇࠆน⢻ᕈ߇㜞޿‫ޕ‬











－ 21 －

PosterNo.13േ‛⚦⢩䈱ᾲ䉴䊃䊧䉴ᖱႎવ㆐䈮㑐ਈ䈜䉎ᣂⷙ䉮䊧䉴䊁䊥䊦䉫䊦䉮䉲䊄䋨CG)วᚑ㉂⚛
䂾⑺ጊ㩷ᄩሶ㪈䇮૒䇱ᧁ㩷ᚑᳯ㪈䇮ự↰㩷ീ㪉䇮⧎Ỉ㩷ୃ๺㪈䇮ᓟ⮮㩷⌀㉿㪈䇮㐳Ⴆ㩷㕏ሶ㪊䇮ዊᨋ㩷ㅴ㪋䇮ᐔᨋ㩷⟵㓶㪊䇮ቶફ㩷䈐䉂ሶ㪈㩷㩷
1䈍⨥䈱᳓ᅚሶᄢቇᄢቇ㒮㩷 ੱ㑆ᢥൻഃᚑ⑼ቇ⎇ⓥ⑼䇮2ർ㒽వ┵⑼ቇᛛⴚᄢቇ㒮ᄢቇ㩷 䊙䊁䊥䉝䊦䉰䉟䉣䊮䉴⎇ⓥ⑼䇮
3ℂൻቇ⎇ⓥᚲ㩷 ⣖⑼ቇ✚ว⎇ⓥ䉶䊮䉺䊷䇮4᧲੩ℂ⑼ᄢቇ㩷 ⮎ቇㇱ㩷 ↢๮ഃ⮎⑼ቇ⑼Introduction
Sterol glucosyltransferase!

Hypothetical stress response reactions in the cell
Heat stress

Sterol glucosyltransferase(Kunimoto et al., Cell Stress & Chaperones, 2000)

GlucoseCholesterol CG itself induced HSF1 activation and following
HSP induction when CG was added to a㩷 㩷㩷 㩷 cultured human fibroblasts.

O

O

Unidentified

OH(Kunimoto et al., Cell Struct. Funct., 2002)

OH

Heat shock transcription
factor 1 (HSF1) activation㩷

Denaturation

OH

Glucose CG administered orally strongly inhibits gastriculcer formation in rats under cold-restraint stress.

Cholesterol(Kunimoto et al., Cell Struct. Funct., 2003)

Heat shock protein (HSP)
production

Intracellular
proteinCG might act as a crucial lipid mediator of HSP induction

CG-synthesizing enzyme, sterol glucosyltransferase, functions as
a thermal stress signal transducer which has not yet been identified.

NormalRefoldingSitosterol
Ergosterol
Poriferasterol
Campesterol
Stigmasterol㩷 etc.

Steryl glucoside

Glucosyltransferase
UDP-glucose:sterol glucosyltransferase (UDPG-SGTase)
Warnecke et al., Plant Mol. Biol. 1997, Warnecke et al., J. Biol. Chem. 1999,
Lebrun et al., J. Biol. Chem., 2006, etc.

Animals
UDPG-SGTase gene has not yet been㩷㩷 identified in animals.
Animal sterol glucosyltransferase gene sharing homology withknown UDPG-SGTase was not found.
Animal sterol glucosyltransferase is considered to bea novel-type enzyme.

Hypothesis of our research
Aggregation

䋫 UDP-glucose

Sterol Heat shock rapidly induced CG formation, andsubsequent HSF1(heat shock transcription
factor 1) activation and HSP induction occurred.

Cholesteryl glucoside
(CG)
CH2OH

Glucose UDP

Plants and fungi䊶䊶䊶

Cholesteryl
glucoside (CG)

Cholesterol

PlasmamembraneHeat stress induced a rapid glycosylation of membrane cholesterolin human fetal lung fibroblast cells, TIG-3.

Purpose of our research

Identification of animal sterol glucosyltransferase

Results
Verification of possible mechanism of
CG formation in the animal cell membrane

Characterization of animal sterol glucosyltransferase
Detection of sterol glucosyltransferase activity
in the animal tissue and cultured cells

Animal sterol glucosyltransferase does not utilize
UDP-glucose as an immediate glucose donor.
In vitro sterol glucosyltransferase assays were performed
without or with UDP-glucose.

Human cultured cells

Isolate thegastric mucosa

Fetal lung fibroblasts 䋺TIG-3

Homogenize

Homogenize

Hog gastric mucosa
extract

Human fibroblasts
extract

S. cerevisiaeCG
Std.㩷 䊷㩷 Mn2+ Cu2+ 䊷㩷 Cu2+ 䊷㩷 Cu2+

*

*

*

*

*

Enzyme reaction

*

*

*

NBD-cholesterol ! ȼ! UDP-Glucose→ NBD-cholesteryl glucoside (CG)The activity of UDPG-SGTase wasincreased in the presence of Me2+.

Lipid analysis

S. cerevisiae

Hog stomach䋫䋫UDP-glucose
CG formation
Relative activity of CG formation (%)

Hog stomach

Using two types of artificially produced liposome mimicking the membranestate before and after CG production, the effects caused by CG formation on
the physical properties and domain thermostability was verified.

㪈㪉㪇

䋭

䋫

䋭

Lipids㩷 㩷 㩷 㩷 㩷

㽲 Before CG
production㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 㩷 (%)

䋭䂓 䋫UDP-glucose䂔 䋭UDP-glucose*

㪈㪇㪇

0.2 mM lipids + 0.6 mM glucose aq.㩷 㩷 Rhodamine-labeled PE: 0.5 mol%

Human fibroblasts

GlcCer

15

0

C16 Ceramide

Cer

0

15

Cholesteryl glucoside㪏㪇㪍㪇㽳 After CG
production

C16 Glucosyl (E) Ceramide

CG

0

15

Cholesterol

chol

25

10

18:1 Phosphatidylcholine

DOPC

40

40

16:0 Phosphatidylcholine

DPPC

20

20

White part : liquid disordered (Ld) phase,the dye rhodamine-labeled PE was seen to be localized at Ld phase.
Circular dark domain :ordered phase which was enriched in glycosphingolipid and cholesterol.

㪋㪇
㪉㪇
㪇

㪈
S. cerevisiae㪉
Hog stomach㪊
Human fibroblasts

surface㽲* 䋺 P䋼0.05 vs 䋭(Mann-Whitney U test) (n=4)

1in-section㽳surfacein-section

2
20͠

Animal sterol glucosyltransferase transfers glucose moiety from GlcCer to cholesterol to form CG.
In vitro sterol glucosyltransferase assays were performed
with various kinds of glycolipid.

Analysis of glucose transfer from GlcCer to cholesterolby hog gastric mucosa extract

Relative activity of CG formation

Examination of the substrate specificity of animal sterol glucosyltransferaseusing fluorescence-labeled cholesterol (NBD-cholesterol).

㪉㪇

10 ɆG

Cholesterol

13C-GlcCer (12C

+

CH2OH

13C

Enzymereaction

Ceramide

CH2OH
O

㪌

+

O

OH

Hog gastricmucosa extract

OH

OH

Solid-ordered phase
Melted at 45͠

Transfer of the glucose moiety from GlcCer to cholesterol changed membrane
physical properties and formed thermostable solid-ordered domains.
6OH

13C-CG㪈㪌

Liquid-ordered phase (lipid raft)
Melted at 35-40͠

OOH(n = 4)

㪈㪇DomainG

HN

O
OH
OH

HO

vs 䋭
* 䋺 (1P 䋼way0.001
ANOVA, Dunnett’s test)*

13
26 C6H61NO8)O䂓 Hog gastric mucosa
䂔 Human fibroblasts

*

㪉㪌

40͠

Conclusion

O
HN
HO

Molecular formula㩷 12C2713C6H56O6
Molecular Weight㩷 554.4

OH

Animal sterol glucosyltransferase is a novel-type enzyme.

㪇㪄䋭㪬㪛㪧㪄㪞㫃㪺Ȼ 㪚㪞
UDP-Glc
DCG

ȼ 㪚㪞
ECG

㪞㫃㪺㪚㪼㫉 GalCer㪞㪸㫃㪚㪼㫉 Sulfatide㪪㫌㫃㪽㪸㫋㫀㪻㪼㪞㪛㪈㪹
GlcCer
G
D1b

4.5e3

Mass spectrometry

4000
3800
3600

599.4 = 593.4 + 6[M䋫HCOO]䋭

(+ ammonium formate)

3400
3200

Novel hypothetical heat stress response reactions in the animal

12C-CG

3.0e3

3000

In te n s ity , c p s

Intensity

Examination of the substrate specificity of animal sterol glucosyltransferaseusing radiolabeled cholesterol ([14C]cholesterol).

13C-CG

Negative mode

4200

4.0e3

3.5e3

analysis

2800

593.4
[M䋫HCOO]䋭

2600

2.5e3

2400
2200

2.0e3

2000

1600

1.5e3

1400
1200

Signaling scaffold

1.0e3

1000
800

0.0

600

555

560

565

570

575

580

585

590

595

600

605
D

610

615

620

625

630

635

640

645

650

655

555 560 565 570 575 580 585 590 595 600 605 610 615 620 625 630 635 640 645 650 655/

Glucose donor䋭㩷

Especially rich in signaling
proteins and lipids

m/z

Hog gastric mucosa

Positive mode

14C-CG

12C-CG +13C-CG

1.0e3

Intensity[(12M+13M)䋫Na]+
1125.82341

2(12C-CG)

0.8e3

Human fibroblasts[2(13M)䋫Na]+
1131.84462

0.4e3

Treatment withheat or membrane fluidizer

0.2e3

Glucosylceramide (GlcCer) was most effective for CG formationby the extracts of hog gastric mucosa and human fibroblasts.

1116 1117 1118 1119 1120 1121 1122 1123 1124 1125 1126 1127 1128 1129 1130 1131 1132 1133 1134 1135 1136 1137 1138 1139

m/z

Lipid rafts isolation

GM95 hGlcT-1㩷

5% Sucrose / TN Buffer
30% Sucrose / TN Buffer

TIG-3

GM95

+

+

Human fibroblasts (TIG-3)
㵺1% TritonX-100 treated
40% Sucrose / TN Buffer

㪍
㪌
㪋
㪊
㪉
㪈
㪇

GlcCer
㪄

TIG-3
㪫㪠㪞㪄㪊

1
0.5
0
-0.5

1

2

3

4

5

1

2

3

4

5

6

7

8

9

10 11

6

7

8

9

10 11

CG formation***
GlcCer㪂㪄Raft marker(Flotillin-1)% Sucrose

Non rafts
㪂

GM95
㪞㪤㪐㪌

Lipid rafts

㪄

48kDa

30

5
top

gradient40
bottom㪂

GM95-hGlcT-1
㪿㪞㫃㪺㪫㪄㪈㫀㫅㩷㪞㪤㪐㪌* 䋺 P 䋼 0.05 (Mann-Whitney U test) (n=4)

HSF1 activation

Transferase

A certain domain,
formed by heat stress,
may act asa signaling platformin heat stress responses
or as a primarydefense mechanism
against the harmful
effects of heat stress.

1.5

39,000rpm, 18h, 4͠͠*Melting of lipid raft2

Fraction+

CG formation?

HSP induction+

GM95 hGlcT-1㩷

Heat stress
Alteration of membrane fluidity

2.5

Protein content (mg)+

Exogenous GlcCer

Relative activityof CG formation

In vitro sterol glucosyltransferase
assays were performed
with or without exogenous GlcCer.

GM95

+

Transferase
Cholesterol
Glycerophospholipid

Alteration of membrane fluidity
Reorganization of lipid rafts

The lipid rafts were isolated from human fibroblasts, TIG-3 cells.

GM-95 cells : a B16 melanoma mutant that does not express ceramide glucosyltransferase (GlcT-1)
GM95-hGlcT-1 cells : GM-95 cells transfected with human GlcT-1 cDNA
TIG-3 cells as a control : Human fetal lung fibroblasts
TIG-3

3

Human sterol glucosyltransferase was localized
at lipid raft fraction.

Enzyme source

UDP-Glucose
GlcCer formation

Sphingomyelin

GlcCer0.0

GlcCer is an essential substrate
for animal sterol glucosyltransferase in the cell.

The expression of GlcT-1 in thecells was confirmed by in vitro
determination of GlcT-1 activity
using C6-NBD-Ceramide andUDP-glucose as a substrate.

GlucoseCeramide

Nagy, E. et al. PNAS, 2007

2(13C-CG)[2(12M)䋫Na]+
1119.80543

0.6e3

Lipid raft

Lipid raft

1800

4

5

－ 22 －

Thermostable domain
GlcCerCG

Transferase

HSF1 activation
HSP induction

Animal sterol glucosyltransferase which localizes at lipid raft may acts asa thermal signal transducer of heat shock responses in the animal cell.

ਛ࿖ߩஜᐽᢎ⢒ߩ⃻⁁ߣᢎ⢒ᢎ᧚ߩ㐿⊒෸߮ᖱႎ౒᦭

Ⲫ䖾㧘ṚỈ౏ሶ㧘ቶફ߈ߺሶ

߅⨥ߩ᳓ᅚሶᄢቇᄢቇ㒮ੱ㑆ᢥൻഃᚑ⑼ቇ⎇ⓥ⑼
᧲੩ㇺᢥ੩඙ᄢႦ 
email: digai@hotmail.co.jp
Keywords: ℂ⑼ᢎ⢒ ஜᐽᢎ⢒ ਛ࿖ߩᢎ⢒ᩰᏅ ᢎ⢒ᢎ᧚ߩ㐿⊒ⷐᣦਛ࿖ߢߪ࿖ജߩべㅴߩ㒶ߢᢎ⢒ᩰᏅ߇↢ߓߡ޿ࠆ‫ޕ‬ਛ࿖ߩᲣߣሶࠍኻ⽎ߣߒᩰᏅߩᤚᱜߦᓎ┙ߟታ〣ࠍⴕ߁ߎߣࠍ⋡ᜰߒߡಽ߆ࠅ߿ߔߊⷫߒߺ߿ߔ޿ℂ⑼ᢎ⢒෸߮ஜᐽᢎ⢒ᢎ᧚ࠍ㐿⊒ߒ࠙ࠚࡉࠍㅢߓߚᖱႎ౒᦭ࠍ࿑ߞߡ޿ࠆ‫ޕ‬⋡⊛ߎࠇ߹ߢᚒ‫⎇ߩޘ‬ⓥቶߢߪ‫⊒ߩߤߥࠕࡘࠡ࡯ࡘ࠾ࠕࡊࡄ߿ࡦ࠲ࠬ࠾ࠟࡈࠕޔ‬ዷㅜ਄࿖ߩሶߤ߽ߣᲣⷫ㆐ߩஜᐽ߳ߩ㑐ᔃࠍ㜞߼ࠆߚ߼ߦ‫ޔ‬ஜᐽᢎ⢒ߩᢎ᧚㐿⊒ࠍⴕߞߡ߈ߚ‫ߩߘޕ‬ਛߩᄙߊߩ߽ߩߪਛ࿖ߩሶߤ߽㆐ߦ߽ᓎ┙ߟߣ⠨߃ࠄࠇࠆ‫ޕ‬ਛ࿖ߢߪ‫⑼ޔ‬ቇᢎ⢒ᝄ⥝ߩ᳇ㆇ߇⪺ߒߊ‫ޔ‬࿖ࠍ᜼ߍߡ⑼ቇᛛⴚߩㅴዷߦข
ࠅ⚵ߺߟߟ޽ࠆ‫ੱޔߒ߆ߒޕ‬ญ߇ᄙߊ‫ޔ‬࿖࿯߇ᐢ޿ߥߤߩ․೎ߥ࿖ᖱ߇޽ࠆߚ
߼ߦ‫ޔ‬ᢎ⢒㕙ߢᄢ߈ߥᩰᏅ߇↢ߓߡ޿ࠆ‫ޕ‬․ߦ‫ޔ‬㐿⊒ߒߚ⑼ቇ⛗ᧄࠍᢎ⢒ߩⴕ߈ዯ߆ߥ޿ጊ㑆ㇱߩሶߤ߽㆐ߩߚ߼ߦᓎ
┙ߡߚ޿ߣ⠨߃‫ޔ‬㐿⊒ࠍㅴ߼ߡ޿ࠆ‫ޕ‬ዊߐ޿ሶߤ߽ߣⷫ߇౒ߦቇ߱ߎߣߢ‫ޔ‬ᣧ޿ᤨᦼ߆ࠄኅᐸᢎ⢒ߦஜᐽߥ↢ᵴߦ㑐ߔࠆ⠨߃ᣇ߇ዉ౉ߐࠇ‫ޔ‬ሶߤ߽㆐߇ቇ㦂ᦼ೨߆ࠄஜᐽ⑼ቇߦ⸅ࠇߡ‫⥝ޔ‬๧ߣ㑐ᔃࠍ⢒߻ߎߣ߇ߢ߈ࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬ਛ࿖ߩጊ㑆ㇱߦ߅ߌࠆஜᐽᢎ⢒ߩ᳓Ḱࠍ਄ߍࠆߎߣࠍ╙৻ߩ⋡⊛ߣߒߡ‫ޔ‬ਛ࿖ߩ࿖ᖱࠍ⠨ᘦߒ‫ޔ‬᭽‫ߥޘ‬႐㕙ߢ૶ߞߡ߽ࠄ߃ࠆ᭽‫⑼ޔ‬ቇ⛗ᧄߩᡷༀࠍⴕߞߡ
޿ࠆ‫੹ޕ‬࿁ߘࠇࠄߩ৻ㇱߦߟ޿ߡ‫ߏޔ‬ႎ๔ߔࠆ‫ޕ‬ᣇᴺ ᒰ⎇ⓥቶߢ૞ᚑߐࠇߚ⛗ᧄ̆‫ޟ‬᳓‫ޟޔޠ‬ᗵᨴ∝‫ޟޔޠ‬㘩‛ߣᩕ㙃‫ߩੱޟޔޠ‬૕ߩ઀⚵ߺ‫ࠍޠ‬ሶߤ߽ะߌߦ‫޿ߒߐ߿ޔ‬ਛ࿖⺆ߦ⠡⸶ߒ‫ޔߦࠄߐޔ‬ᣣᧄߦṛ࿷ߒߡ޿ࠆ⇐ቇ↢߿৻⥸ߩᣇ‫ߚ߹ޔޘ‬ਛ࿖ᧄ࿯߆ࠄ⎇ୃߦ᧪ߚᢎᏧߣ↢ᓤߦ⺒ࠎߢ߽ࠄߞߡ‫ޔ‬ᗧ⷗ࠍᓧߚ਄ߢᡷༀࠍⴕߞߚ‫ޕ‬⚿ᨐ ⺞ᩏ࡮ಽᨆࠍㅢߒߡᶋ߆߮਄߇ߞߡ߈ߚ໧㗴ߦၮߠ߈‫ޔ‬એਅߦ߹ߣ߼ߚࠃ߁
ߦ⛗ᧄࠍቢᚑߒ‫ߦࠄߐޔ‬ౝኈℂ⸃ߒ߿ߔߊߔࠆߚ߼ߦታ㛎ᦠࠍ૞ᚑߒߡ޿ࠆ‫ޕ‬－ 23 －

⺞ᩏ࡮ಽᨆߩ⚿ᨐਛ࿖ߩ⟵ോᢎ⢒Ბ㓏ߦ߅ߌࠆ⑼ቇᢎ⢒෸߮ஜᐽᢎ⢒ߩ⃻⁁ߦߟ޿ߡ⺞ᩏ࡮ಽᨆࠍⴕߞߚ
⑼ቇᢎ⢒(ℂ⑼ᢎ⢒)ߦ߅ߌࠆ໧㗴¾ ᢎᏧ↪ߩᜰዉᦠ߇᦭ലߦ૶ࠊࠇߡ޿ߥ޿
¾ ⇣ߥࠆኾ㐷ኅߦࠃࠆᜰዉℂᔨ߿ᣇᴺ߇৻⥌ߒߥ޿
¾ ᢎᏧߩᢎ⢒࡟ࡌ࡞ߦኻߔࠆ⹏ଔߪ‫ޔ‬ᢎ⢒ℂᔨߢߪߥߊ‫⋧ޔ‬ᄌࠊࠄߕ⍮⼂ᢎ⢒ߩ㆐ᚑ⋡ᮡߦၮߠ޿ߡ޿ࠆ
¾ ᢎ᧚ߦઃዻߔࠆᢎౕߩ⾰߇ᖡ޿ߎߣ߿ᢎቇౝኈߦวࠊߥ޿ߥߤߦࠃߞߡ‫߇₸↪૶ޔ‬ૐ޿ஜᐽᢎ⢒ߦ߅ߌࠆ໧㗴¾ ߶ߣࠎߤߩቇᩞߦஜᐽⴡ↢⍮⼂߇ࠊ߆ࠆᢎᏧ߇޿ߥ޿ߚ߼‫ޔ‬ஜᐽᢎ⢒᝼ᬺࠍᜂᒰߔࠆኾછ
ᢎᏧ߇߅ࠄߕ‫ޔ‬ᢎ⢒⋡ᮡߦኻᔕߢ߈ߥ޿
¾ ஜᐽᢎ⢒᝼ᬺߦ⋧ᔕߒ޿ᢎ᧚߇ߥ޿
¾ ᝼ᬺߩᤨ㑆߇ઁ⑼⋡ߦᵹ↪ߐࠇࠆߎߣ߇ᄙ޿

⛗ᧄߩ࠹࡯ࡑ૞ᚑߩ⋡ᮡታ㛎⛗ᧄߩ࠹࡯ࡑ

ࠦࡦ࠹ࡦ࠷ੱ૕ߩ᭴ㅧߣᯏ⢻ੱߩ૕ߩ઀⚵ߺ㘩‛ࠍ៨ขߔࠆߎߣߩ

㧔⣖‫ޔ‬㛽ᩰ‫╭ޔ‬⡺‫ޔ‬ᔃ࡮ໜᶧߢ࠺ࡦࡊࡦ

ᗧ๧‫ޔ‬ᩕ㙃ߣࠛࡀ࡞ࠡ࡯‫ޔ‬⤳ߣⴊ▤‫ޔ‬๭ๆེ‫ ޔ‬ᶖࠍ♧ߦᄌ߃ࠃ߁

૕ߩᚑ㐳‫ޔ‬ᩕ㙃ਇ⿷ߦൻེ‫ޔ‬ᴲዩེ‫ ޔ‬ᗵⷡ࡮ࡅ࠻ߣᬀ‛ߩ๭ࠃߞߡ߽ߚࠄߐࠇࠆ∔∛ེ‫↢ޔ‬ᱺེ㧕ࠍ ℂ⸃ๆࠍᲧセߒߡߺ

ࠍ⍮ߞߡஜᐽߥ↢ᵴࠍㅍߒ‫⥄ ޔ‬ಽߩ૕ߩ ߪߚࠃ߁ߩᗵᨴ⚻〝‫੍ޔ‬㒐ᴺߦߟ޿ߡℂ⸃ߒ‫∛ޔ‬᳇ߦߥ ࠄ ߥ ޿ ߚ߼ ߩ⍮ ⼂ࠍりߦߟߌߡ߶ߒ޿

ࠆߚ߼ߩ⍮ᕺࠍりߦߟߌ



㘩‛ߣᩕ㙃

ࠄ߈ࠍ⍮ߞߡ߶ߒ޿৻ ⥸ ⊛ ߥ ᗵᨴ ∝߿ ߘ



૞ᚑߩ⋡ᮡ

ߡ߶ߒ޿

ታ㛎
ࠦࡦ࠹ࡦ࠷
࡮ᩕ㙃㘩ࠍ૞ߞߡ
ߺࠃ߁࡮࡛࡯࠼࠺ࡦࡊࡦ
෻ᔕ࡮りㄭߥ㊁⩿ࠍ⺞ߴࠃ߁᳓ߩ㊀ⷐᕈߣ਎⇇ਛߩ

࡮ᚻᵞ޿ߩലᨐࠍ

᳓⾗Ḯ෸߮᳓ࠍ߈ࠇ޿ߦ ࡮᳓ߩ◲ᤃᵺൻ⏕߆߼ࠃ߁ߔ ࠆ ߎ ߣ ߩ ᗧ ๧ ࠍ ⍮ ߞ ࡮᷷ᐲߦࠃࠆ᳓ߩ

࡮ᾚᴣߩᶖᲥലᨐ

ߡ‫ޔ‬ⅣႺ߳ߩ㑐ᔃࠍ㜞߼ࠍ⏕߆߼ࠃ߁

ߡ߶ߒ޿



ᗵᨴ∝

ᄌൻࠍ⍮ࠈ߁᳓                       ⠨ኤ ࿾ၞߩᢎ⢒ᩰᏅシᷫߦᓎ┙ߡࠆߚ߼ߦ‫ޔ‬ஜᐽᢎ⢒ߩ⵬ഥᢎ᧚ߣߒߡ‫ޔ‬ᚒ‫߇ޘ‬૞ᚑߒߚ⛗ᧄࠍᵴ↪ߒߡ߽ࠄ߁ߎߣࠍ⠨߃ߡ޿ࠆ‫ޕ‬ਛ࿖߆ࠄ᧪ᣣߒߡ޿ࠆᣇ‫ޘ‬
㧔ℂ⑼ᢎຬ‫⑼ޔ‬ቇ㙚㙚㐳‫ޔ‬කᏧ‫↢┬ఽޔ‬ᓤ‫  ⚂⸘ޔ‬ฬ㧕߆ࠄએਅߩᗧ⷗ࠍ㗂޿ߚ‫ޕ‬࡮ጊ㑆ㇱߩሶߤ߽㆐ߦߪ‫ߩࠄࠇߎޔ‬⍮⼂߇ਇ⿷ߒߡ޿ࠆߩߢ‫ޔ‬㕖Ᏹߦᓎ┙ߟߛࠈ߁‫ޕ‬
࡮ᐜ⒩࿦ఽ߆ࠄዊቇᩞਛቇᐕߊࠄ޿߹ߢߩሶߤ߽㆐ߦ೑↪น⢻‫ޕ‬ౝኈߩ⒟ᐲ߇
㜞޿߽ߩߪਛቇ↢߹ߢ‫ޕࠆ߈ߢ↪૶ޔ‬࡮ቇᩞߦ஻߃ࠆߎߣߢ᦭ലᵴ↪ߢ߈ࠆ‫ޕ‬－ 24 －

࡮⑼ቇ⊛ߥ⸒⪲ߩ⺑᣿߇ⷫಾߢ‫߇⛗ޔ‬ℂ⸃ࠍ⵬ߞߡ޿ࠆ‫ޕ‬࡮ቇᩞߢ೑↪ߔࠆ႐วߪ‫ޔ‬ᢎ⢒ᆔຬળࠍㅢߔ߶߁߇቟ᔃߢ޽ࠆ‫ޕ‬ᦝߦᗧ⷗߿ᗵᗐࠍ᳞߼‫⑼ߩࠄࠇߎޔ‬ቇ⛗ᧄ߇᦭ലߦ೑↪ߒߡ߽ࠄ߃ࠆࠃ߁ߦ
ߒߚ޿‫ޕ‬߹ߣ߼ ᚒ‫߇ޘ‬૞ᚑߒߚ⛗ᧄߪ‫ޔ‬ᣢߦ⊒ዷㅜ਄ߩࠕࡈࠟ࠾ࠬ࠲ࡦߣࡄࡊࠕ࠾ࡘ࡯ࠡࡘ
ࠕߢ૶ࠊࠇ‫ޔ‬࿾ၞߩᢎ⢒⠪߆ࠄᅢ⹏ࠍᓧߡ޿ࠆ‫ࠍᧄ⛗ߩߎޕ‬ਛ࿖ߢ߽೑↪ߢ߈ࠆࠃ߁ߦ‫ޔ‬࿖ᖱߦวࠊߖߡᡷༀߒ‫ޔ‬ሶߤ߽㆐ߩ଻ஜⴡ↢ߩะ਄ࠍ࿑ࠅ‫ޔ‬࿾ၞᩰᏅ߆ࠄ↢ߓࠆஜᐽᢎ⢒ߩᩰᏅᤚᱜߦᓎ┙ߡߚ޿ߣ⠨߃ߡ޿ࠆ‫ ޕ‬ᐕ  ᦬ߦ‫ޔ‬⁛┙ⴕ᡽ᴺੱ⑼ቇᛛⴚᝄ⥝ᯏ᭴㧔,56㧕ߩਛ࿖✚ว⎇ⓥ࠮ࡦ࠲࡯ߦࠃࠆਛ࿖⺆ࡐ࡯࠲࡞ࠨࠗ࠻ߦ2&( ࠍឝタߒ‫ޔ‬ਛ࿖ߩᢎ⢒㑐ଥ⠪ߩ೑↪ߦଏߔࠆ੍ቯߢ޽ࠆ‫ޕ‬੹ᓟߩዷᦸ
ਛ࿖ߩ⚻ᷣ߇㘧べ⊛ߦ⊒ዷߔࠆߣ౒ߦ‫ޔ‬࿖᳃ߩஜᐽߦኻߔࠆ㑐ᔃ߽㜞߹ߞߡ߈ߡ޿ࠆ‫ߩߘޕ‬ਛߢ‫ޔ‬ᐜዋᤨ߆ࠄߩஜᐽᢎ⢒߇㊀ⷞߐࠇࠆࠃ߁ߦߥࠆߎߣ߇੍
ᗐߐࠇ‫ޔ‬ஜᐽᢎ⢒ౝኈ߽߶߆ߩᢎ⑼ߣหߓࠃ߁ߦቇᩞ⃻႐ߩ᝼ᬺߣߒߡታ〣ߐࠇࠆࠃ߁ߦߥࠆߎߣ߇⠨߃ࠄࠇࠆ‫ޕ‬ᚒ‫߇ޘ‬૞ᚑߒߚ⛗ᧄ߿ታ㛎ౠሶߪ‫ޔ‬ਛ࿖⑼ቇᛛⴚදળ߆ࠄ߽㜞޿⹏ଔࠍฃߌࠆߎߣ߇ߢ߈‫ߦࠄߐޔ‬ਛ࿖߆ࠄ੤ᵹߩ⋡⊛ߦ᧪ᣣߒߚᢎᏧ߿ሶߤ߽㆐߆ࠄ߽ᅢ⹏ࠍᓧߡ޿ࠆ‫ޕ‬หදળߩදജࠍᓧߡ‫ޔ‬ਛ࿖ߦ߅ߌࠆዞቇ೨ߩቇ⠌߿‫ޔ‬ዊቇᩞ╬ߩ᝼ᬺߩℂ⸃ࠍഥߌࠆ⾗ᢱߣߒߡᵴ↪ߔࠆߎߣࠍ⋡⊛ߣߒߡ࠙ࠚࡉࠍㅢߓߡᖱႎឭଏ╬ࠍ࿑ߞߡ޿ࠆ‫ޕ‬ෳ⠨ᢥ₂
A BOOK ABOUT LIFE : FOR YOUR WELĹBEING, Kimiko Murofushi & Kimiko
Takizawa, Ochanomizu Academic Association NPO, 2009－ 25 －

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