Mass spectrometry‐ based metabolomics
K Dettmer, PA Aronov… - Mass spectrometry …, 2007 - Wiley Online LibraryThis review presents an overview of the dynamically developing field of mass spectrometry-based metabolomics. Metabolomics aims at thecomprehensive and quantitative analysis of wide arrays of metabolites in biological samples. These numerous analytes have very …
Cited by 1476

Gas chromatography/mass spectrometry in metabolic profiling of biological fluids
KK Pasikanti, PC Ho, ECY Chan - Journal of Chromatography B, 2008 - Elsevier

One of the objectives of metabonomics is to identify subtle changes in metabolite profiles between biological systems of different physiological or
pathological states. Gas chromatography mass spectrometry (GC/MS) is a widely used analytical tool for metabolic …
Cited by 258

Principal component analysis of urine metabolites detected by NMR and DESI–MS in patients with inborn
errors of metabolism
Z Pan, H Gu, N Talaty, H Chen, N Shanaiah… - Analytical and …, 2007 - Springer
Urine metabolic profiles of patients with inborn errors of metabolism were examined with nuclear magnetic resonance (NMR) and desorption
electrospray ionization mass spectrometry (DESI–MS) methods. Spectra obtained from the study of urine samples from …
Cited by 163

Metabolomics in the study of kidney diseases
RH Weiss, K Kim - Nature Reviews Nephrology, 2012 - nature.com
Metabolomics—the nontargeted measurement of all metabolites produced by the body—is beginning to show promise in both biomarker discovery
and, in the form of pharmacometabolomics, in aiding the choice of therapy for patients with specific diseases. In …
Cited by 169[BOOK] Metabolome analysis: an introduction

SG Villas-Boas, J Nielsen, J Smedsgaard… - 2007 - books.google.com
Providing information on the main approaches for the analysis of metabolites, this textbook: Covers basic methodologies in sample preparation andseparation techniques, as well as the most recent techniques of mass spectrometry. Differentiates between primary and …
Cited by 143

Mass spectrometry‐ based holistic analytical approaches for metabolite profiling in systems biology studies
G Theodoridis, HG Gika… - Mass spectrometry reviews, 2011 - Wiley Online Library
Metabonomics and metabolomics represent one of the three major platforms in systems biology. To perform metabolomics it is necessary togenerate comprehensive “global” metabolite profiles from complex samples, for example, biological fluids or tissue extracts …
Cited by 132

Recent and potential developments in the analysis of urine: a review
D Ryan, K Robards, PD Prenzler, M Kendall - Analytica Chimica Acta, 2011 - Elsevier
Abstract Analysis of urine is a widely used diagnostic tool that traditionally measured one or, at most, a few metabolites. However, the recognition of
the need for a holistic approach to metabolism led to the application of metabolomics to urine for disease diagnostics. This …
Cited by 123[HTML] Ejaculatory disorder caused by alpha‐ 1 adrenoceptor antagonists is not retrograde ejaculation but aloss of seminal emission
SI HISASUE, R Furuya, N Itoh… - … journal of urology, 2006 - Wiley Online Library

Aim: The etiology of the ejaculatory disorder induced by alpha-1 blockers is still controversial, although it has been suggested to be retrograde
ejaculation. The aim of this study was to investigate the distribution of alpha-1 adrenoceptor subtype mRNA in human …
Cited by 115

Metabolomics study of stepwise hepatocarcinogenesis from the model rats to patients: potential biomarkers
effective for small hepatocellular carcinoma diagnosis
Y Tan, P Yin, L Tang, W Xing, Q Huang, D Cao… - Molecular & Cellular …, 2012 - ASBMBThe aim of this study is to find the potential biomarkers from the rat hepatocellular carcinoma (HCC) disease model by using a non-targetmetabolomics method, and test their usefulness in early human HCC diagnosis. The serum metabolic profiling of the diethylnitrosamine …
Cited by 104

Inhibition of Seminal Emission Is the Main Cause of Anejaculation Induced by a New Highly Selective
α1A‐ Blocker in Normal Volunteers
K Kobayashi, N Masumori, S Hisasue… - The journal of sexual …, 2008 - Wiley Online LibraryAim. We evaluated the effect of a new, highly selective α1A-blocker, silodosin, on ejaculatory function of normal volunteers. Methods. The study
included 15 healthy male urologists who voluntarily participated in the study. They took 4 mg of silodosin or a placebo twice daily for 3 …
Cited by 88

Monitoring the health to disease continuum with global metabolic profiling and systems biology
LK Schnackenberg, RD Beger - 2006 - Future Medicine
Global metabolic profiling, which includes both metabolomics and metabonomics studies, is the latest 'omics' research platform that is being applied
to understand the health and disease continuum. Metabolic profiling analyses have been demonstrated for the …
Cited by 88

A practical approach to detect unique metabolic patterns for personalized medicine
JM Johnson, T Yu, FH Strobel, DP Jones - Analyst, 2010 - pubs.rsc.org
Information-rich technologies have advanced personalized medicine, yet obstacles limit measurement of large numbers of chemicals in humansamples. Current laboratory tests measure hundreds of chemicals based upon existing knowledge of exposures, metabolism …
Cited by 88

Serum metabonomics study of chronic renal failure by ultra performance liquid chromatography coupled with
Q-TOF mass spectrometry
L Jia, J Chen, P Yin, X Lu, G Xu - Metabolomics, 2008 - Springer
A metabonomics technique based on ultra-performance liquid chromatography (UPLC) coupled with Q-TOF mass spectrometry was employed toinvestigate the sera from 32 patients with chronic renal failure (CRF) without renal replacement therapy and 30 healthy …
Cited by 79

Future of liquid chromatography–mass spectrometry in metabolic profiling and metabolomic studies forbiomarker discovery
TO Metz, Q Zhang, JS Page, Y Shen, SJ Callister… - 2007 - Future Medicine

The future utility of liquid chromatography–mass spectrometry (LC–MS) in metabolic profiling and metabolomic studies for biomarker discovery isdiscussed, beginning with a brief description of the evolution of metabolomics and the utilization of the three most …
Cited by 73

Noninvasive human metabolome analysis for differential diagnosis of inborn errors of metabolism
T Kuhara - Journal of Chromatography B, 2007 - Elsevier

Early diagnosis and treatment are critical for patients with inborn errors of metabolism (IEMs). For most IEMs, the clinical presentations are variable
and nonspecific, and routine laboratory tests do not indicate the etiology of the disease. A diagnostic procedure using …
Cited by 61

Metabolic profiling of infant urine using comprehensive two-dimensional gas chromatography: application tothe diagnosis of organic acidurias and biomarker …
KA Kouremenos, J Pitt, PJ Marriott - Journal of Chromatography A, 2010 - Elsevier
Comprehensive two-dimensional gas chromatography (GC× GC) time-of-flight mass spectrometry (ToFMS) was applied to the analysis of urinaryorganic acids from patients with inborn errors of metabolism. Abnormal profiles were obtained from all five patients studied …
Cited by 62

Serum metabonomics study of adenine-induced chronic renal failure in rats by ultra performance liquid
chromatography coupled with quadrupole time-of-flight mass …
YY Zhao, XL Cheng, F Wei, XY Xiao, WJ Sun… - Biomarkers, 2012 - Taylor & Francis
An ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC Q-TOF MS) metabonomics approach
was employed to study the serum metabolic profiling of adenine-induced chronic renal failure (CRF) rats. Acquired data were …

Cited by 63

Computational strategies for metabolite identification in metabolomics
DS Wishart - Bioanalysis, 2009 - Future Science
Most metabolomic data are characterized by complex spectra or chromatograms containing hundreds of peaks or features. While there are manymethods for aligning or comparing these spectral features, there are few approaches for actually identifying which peaks match …
Cited by 60

Mass spectrometry-based technologies for high-throughput metabolomics
J Han, R Datla, S Chan, CH Borchers - Bioanalysis, 2009 - Future Science
The metabolome is composed of a vast number of small-molecule metabolites that exhibit a diversity of physical and chemical properties and exist
over a wide dynamic range in biological samples. Multiple analytical techniques, used in a complementary manner, are …
Cited by 58

Selective screening for inborn errors of metabolism and secondary methylmalonic aciduria in pregnancy athigh risk district of neural tube defects: a human …
YZ Song, BX Li, H Hao, RL Xin, T Zhang, CH Zhang… - Clinical …, 2008 - Elsevier
Objective Urease pretreatment-gas chromatography-mass spectrometry (UP-GC-MS) has become a valuable tool in the field of metabolome
research, including analysis of inborn errors of metabolism (IEMs) and acquired metabolic disturbances secondary to nutrition or …
Cited by 57

An automated method for peak detection and matching in large gas chromatography‐ mass spectrometry
data sets
SJ Dixon, RG Brereton, HA Soini… - Journal of …, 2006 - Wiley Online Library

A new approach for peak detection and matching has been developed and applied to two data sets. The first consisted of the Gas
Chromatography-Mass Spectrometry (GC-MS) samples of 965 human sweat samples obtained from a population of 197 individuals. The …
Cited by 57

Influences of methamphetamine-induced acute intoxication on urinary and plasma metabolic profiles in therat
N Shima, I Miyawaki, K Bando, H Horie, K Zaitsu… - Toxicology, 2011 - Elsevier
Methamphetamine (MA) is an illicit psychostimulant, and its abuse has become an international public health problem. MA intoxication can cause
life-threatening hyperthermia, renal and liver failure, cardiac arrhythmias, and neurological damage. To investigate the …
Cited by 45

Global metabolic profiling and its role in systems biology to advance personalized medicine in the 21stcentury
LK Schnackenberg - Expert Review of Molecular Diagnostics, 2007 - Taylor & Francis

Systems biology attempts to elucidate the complex interaction between genes, proteins and metabolites to provide a mechanistic understanding ofcellular function and how this function is affected by disease processes, drug toxicity or drug efficacy effects. Global metabolic …
Cited by 42[HTML] Untargeted metabolomic analysis for the clinical screening of inborn errors of metabolism
MJ Miller, AD Kennedy, AD Eckhart, LC Burrage… - Journal of inherited …, 2015 - Springer
Global metabolic profiling currently achievable by untargeted mass spectrometry-based metabolomic platforms has great potential to advance ourunderstanding of human disease states, including potential utility in the detection of novel and known inborn errors of …
Cited by 55

Multiwell plates loaded with fluorescent hydrogel sensors for measuring pH and glucose concentration
B Vilozny, A Schiller, RA Wessling… - Journal of Materials …, 2011 - pubs.rsc.org
Fluorescent hydrogels were polymerized directly in multi-well plates at ambient temperature and in the presence of air, producing sensors formeasuring pH and glucose concentration. The plates were rapidly analyzed using a fluorescence plate reader. Multiwell pH sensors …
Cited by 35

The application of mass spectrometry to proteomics and metabolomics in biomarker discovery and drug
development
T Mikami, M Aoki, T Kimura - Current molecular pharmacology, 2012 - ingentaconnect.com

Drugs are launched to market after the lengthy process of development. Despite careful preclinical assessment, there is still a significant risk that adrug candidate may elicit adverse effects or display a low level of efficacy during clinical trials. If a drug candidate fails in the …
Cited by 39

Direct sample injection for capillary electrophoretic determination of organic acids in cerebrospinal fluid
R Ramautar, GW Somsen, GJ de Jong - Analytical and bioanalytical …, 2007 - Springer
Organic acids in cerebrospinal fluid (CSF) are potential diagnostic markers for neurological diseases and metabolic disorders. A capillaryelectrophoretic (CE) method for the direct analysis, ie, without any sample preparation, of six organic acids in CSF was developed. A …
Cited by 30

Biomarkers for neuroAIDS: the widening scope of metabolomics
G Pendyala, EJ Want, W Webb, G Siuzdak… - Journal of Neuroimmune …, 2007 - Springer
Abstract “Metabolomics”, the measurement of metabolite concentrations and fluxes in cell systems, is an emerging science that has enormous
potential and several unique characteristics. The current applications for this field are, primarily, toxicological profiling and …
Cited by 30

Metabolomic biomarkers: their role in the critical pathLK Schnackenberg, RD Beger - Drug Discovery Today: Technologies, 2007 - Elsevier
Global metabolic profiling is being applied to identify biomarkers of health. Some small molecules are exquisitely sensitive indicators of health status.
Metabolic profiling analyses are being used to determine biomarkers of drug safety and effectiveness as well as disease …
Cited by 29

Application of supercritical fluid chromatography to the analysis of hydrophobic metabolites
T Bamba - Journal of separation science, 2008 - Wiley Online LibraryThis review describes the usefulness of supercritical fluid chromatography (SFC) in the analysis of hydrophobic metabolites. The use of SFC for theanalysis of naturally occurring polyprenols markedly improves the chromatographic resolution of polyprenol homologues …
Cited by 26