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Product Name:

AllView PAGE Buffer

Code No:

DS520

Size:

500 ml (20 × stock solution)

Storage:

Store at Room Temperature (Protect from light)

Stability:

2 years at Room Temperature

Description
AllView PAGE Buffer is a new type of running buffer for SDS-PAGE electrophoresis. This buffer has aremarkable feature that enable us to separate proteins with wide range of molecular weights in the basic
Laemmli gel (Tris-HCl) similar to using “gradient gel”. The recommended acrylamide concentration is 6%for resolving gel and 3% for stacking gel to separate the entire range of about 10 kDa to 250 kDa. Afterelectrophoresis, the polyacrylamide gel can be used directly for CBB staining, silver staining or western
blotting.
Protocol1.

Dilute the AllView PAGE Buffer 20 times with ultrapure water.
(e.g. Add 25 ml of AllView PAGE Buffer to 475 ml of ultrapure water.)2.

Set the gel in electrophoresis chambers.

3.

Fill the chambers with the 1 × AllView PAGE Buffer.

4.

Load your samples and molecular weight markers.

5.

Start electrophoresis.

GelVoltage

TimeMini gel

250 V15 min(8 × 10 cm, 1 mm thick)(Constant voltage)

Note: AllView PAGE Buffer is suitable for Laemmli gels (see below “Recommended usage”).
 If precast gels are used, the optimal acrylamide concentration may be different.
 AllView PAGE Buffer is not reusable.
 AllView PAGE Buffer is 20 × stock solution. Please dilute to 1 × solution before use.
 If precipitation of SDS is observed, completely dissolve it in a water bath (about 37°C) before use.
 Optimal electrophoretic time is depending on acrylamide concentration, gel size and voltage.
Accordingly, it is recommended to monitor electrophoresis using a prestained MW Marker(e.g. DynaMarker Protein MultiColor Stable II, Code#DM660).
 Sometimes the gel become too hot during electrophoresis with two gels at the same time or with a large

size gel. To avoid heat generation, use the chilled AllView PAGE Buffer and electrophorese in a coldroom.

1
BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.2.5

Recommended usage
The recommended acrylamide concentration is 6% for resolving gel and 3% for stacking gel to separate theentire range of about 10 kDa to 250 kDa. Especially if the separation of low molecular weight proteins (10kDa~30 kDa) is required, 10% resolving gel is recommended.
1.

Gel preparation (Laemmli’s method)

Table 1. Recipes for polyacrylamide resolving and stacking gel (2 mini gels)
Stacking gel (6 ml)

Resolving gel (15 ml)

Gel percentage

3%6%

10%

Ultrapure water

3.9 ml

8.05

6.05

3.75

3.75

1.5M Tris-HCl (pH8.8)
0.5M Tris-HCl (pH6.8)

1.5

30% Acrylamide/Bis solution

0.6

3.0

5.0

10% SDS

0.06

0.150.15

TEMED

0.003 (3 µl)

0.00375 (3.75 µl)

0.00375(3.75 µl)

10% APS

0.06

0.05

0.05

Stacking gel
Resolving gel

3%6%

10%kDa*230
14110071453025178.4* DynaMarker Protein MultiColor Stable II, Code#DM660

2BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.2.5

2.

Electrophoretic image

The mobility of proteins using AllView PAGE Buffer is different from Laemmli electrophoresis runningbuffer (Tris-Glycine-SDS). By using AllView PAGE Buffer and the Laemmli gels (Tris-HCl), a wide rangeof protein sizes can be separated like a gradient gels.
Stacking gel

3%Resolving gel
Buffer6%

10%

Tris-Glycine-

AllView PAGE

Tris-Glycine-

AllView PAGE

SDS buffer

BufferSDS buffer

Buffer230

230 (kDa)*

230

230

45
2545

8.4

8.4

25

* DynaMarker Protein MultiColor Stable II, Code#DM660

Related Products
Code
Product Name
DM660 DynaMarker Protein MultiColor Stable II

Description
The DynaMarker Protein MultiColor Stable II is a
pre-stained protein molecular weight marker.
The marker has a remarkable feature that it ispossible to store at 4 °C.

DS500QuickBlue Staining Solution

QuickBlue Staining Solution stains proteins inpolyacrylamide gel after SDS gel electrophoresis(detection limit >= 8 ng of protein) with
*Coomassie®-G250. All processes including
washing and destaining processes can be
performed in 1.5 hr.

3
BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.2.5