Surface Plasmon ResonanceLabel-Free Analysis System

BI-4500 Series
5 channel SPR with modular
design for applications flexibility✧ High throughput with 5 channels and fully automated sampling✧ High sensitivity to measure small molecules✧ Precise sample delivery with BI-DirectFlowTM technology✧ Innovative multi-module design for optimal flexibility✧ Cost effective solution

The new BI-4500 SPR system provides multiple channel flow modes and delivers high quality binding
response for low immobilization and small molecule (<100 Da) detection. Equiped with BI-DirectFlowTM
technology, the BI-4500 system integrates precision sample delivery with near-zero dispersion for fast
kinetics and effective removal of various secondary effects. Its modular innovative design gives users withoptimal flexibility to choose amongst various analysis modules for life science, electrochemistry, andsensing in liquid and gas phase SPR applications.

Precise sample delivery
with BI-DirectFlowTM

Life Science Applications

BI-DirectFlow™ technology delivers
sample to the sensor surface withnear-zero dispersion generatinghigh quality data that more clearly
distinguishes true binding eventsfrom the secondary effects.

Interaction between Bovine Serum Albumin (BSA)and Anti-BSA can be monitored in real-time byusing the flow injection SPR analysis module.

The binding response on the left (without dispersion) hasvery sharp, well-defined binding analysis regions andgenerates more accurate and reproducible results. The
binding response on the right (with dispersion) has blurred,
poorly defined binding analysis regions.

Material Science Applications
Metal Deposition/Stripping in EC SPR
Using EC SPR to quantify the amount of metalelectrodeposited onto a surface, the thicknesses ofthe copper film can be determined within subangstrom precision. The ability of EC SPR todetermine tiny thickness variations down to subangstrom level demonstrates its superb sensitivity.

EC SPR study of 5 mM CuSO4/0.1 M H2SO4 solution:(a) cyclic voltamagram showing copper redox peaks(b) simultaneous SPR response confirming copper film
deposition and stripping corresponding to the redox
potentials.

Binding Kinetics Analysis

Reference subtracted binding curves of four channels atvarying analyte concentrations with kinetic analysis fits
showing an associate rate constant ka=8.6 × 104 ± 0.5 M-1s-1,dissociation rate constant kd=1.5 × 10-4 s-1 ± 0.25, andaffinity binding constant KD=1.7 nM ± 0.2

4500 System Specifications

Base Station

Fluid HandlingLight source

670 nmDetection speed

4 ms

Incident angles

40-47 Deg (gas)67-81 Deg (liquid)

Baseline noise< 0.06 RU RMS (0.01 mDeg RMS)

Baseline drift

0.30 RU/hr (0.05 mDeg/hr) (when ambient drifts < 1°C/hr)

Temperature Control Range

6°C to 50°C (10°C below ambient temperature max)Outer dimension355(w) x 250 (h) x 515 (d) mm

Weight11.5 kgPower supply

110-230 V 50/60 Hz

Number of sample flow channels

5 channels

Flow cell material

PEEK (biologically compatible)

Flow rate

1.0 to 250 μL/min (application dependent)

Sample injection volume>50 μL (application dependent)

Sample injection methods

Fully automated (Autosampler option)
Semi-automatedKinetic constantk a <1 X 10 8 M-1s-1
k d >1 X 10 -6 s -1

Dissociation constant

K D = 10 -3 M (1 mM) to 10-12 M (1 pM)

Molecular weight cutoff

100 DaComputerWindows operating system

Software

BI-SPR software including Data Analysis and Kinetics Analysis packages

Sample capacity

2 x SBS standards (384 / 96), 2 x 48 Vials (1.5mL), 2 x 12 Vials (10mL)

Sample cooling

Minimum: 4o C +/- 2 o C

Outer dimension

300 (w) x 575 (h) x 360 (d) mm

Weight21 kg

Buffer exchange

Automatic buffer exchange up to six sources

Buffer degasser

In-lineBuffer delivery

Continuous

Outer dimension

305 (w) x 191 (h) x 330 (d) mm

Weight6.8 kgControl System

Autosampler(option)

Automatic Buffer Exchange
Pump and Degasser(option)

Analysis Modules
BI-DirectFlowTM
This module enables precise flow control technology that delivers sample to the sensor surface withnear-zero dispersion, enabling the study of SPR events in greater detail than ever before.
EC-DualFlowTM
This module provides users with novel capabilities to study molecular binding processes andconformational changes of biomolecules under the influence of applied electrochemical potentials atdifferent flow rates. Its small channel volume facilitates rapid sample exchange and fast kinetic studies.
EC SPR
This module facilitates simultaneous electrochemical and SPR measurements on the same sensor chip,and is ideal for studying various electrochemical processes with SPR.
Gas SPR
This module enables the high sensitivity of SPR analysis to be performed in the gas phase, permitting
new capabilities for sensor development, thin film analysis, and gas molecule binding studies.

BI-DirectFlowTM

EC-DualFlowTM

EC SPR

Gas SPR

Included

Optional

Optional

Optional

Sensor Chips
Bare Gold Sensor Chip
Highly uniform gold film for reproducible SPR research.
Divided Gold Sensor Chip
Pre-patterned gold surface for EC flow SPR applications.
CM Dextran Sensor Chip
Sensor with COOH- linker groups in a dextran hydrogel, ideal for high capacity amine coupling with low
non-specific absorption.
Streptavidin (SA) Sensor Chip
Sensor with streptavidin in a dextran hydrogel for immobilization of biotinylated molecules such asproteins, peptides, nucleic acids or carbohydrates.
Ni-NTA Sensor Chip Sensor Chip
Sensor with NTA used for immobilizing histidine-tagged molecules. NTA surface can be regenerated
by injecting EDTA or imidazole.
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Website: www.BiosensingUSA.com
Tel: 1-480-491-2777 Fax: 1-866-897-8741© Biosensing Instrument Inc. 2018

Product specification and descriptions in this document subject to change without notice