Call for applications: 2017 Joint Usage and Joint Research Programs, Institute ofAdvanced Medical Sciences, Tokushima University
30th January 2017
Tokushima University, also known as University of Tokushima, has a long history ofadvanced medical sciences. Miyoshi muscular dystrophy and adjuvant disease werediscovered here. Immunoproteasomes, hepatocyte growth factor, and cathepsin L as
well as many other disease-related human molecules have their home in our campus. In
1961, the medical school of Tokushima University founded three laboratories of enzyme
research, which successfully formed Institute for Enzyme Research in 1987. In 1998,Institute for Genome Research was founded to extend medical research to focus on
genome functions. In 2010, Diabetes Therapeutics and Research Center was formed toovercome the diabetes by creating platform for the collaborations between clinical andbasic researchers. In 2013, Fujii Memorial Institute of Medical Sciences was founded inmemory of Professor Setsuro Fujii, one of the founding professors of original enzyme
research laboratories. In 2016, the University has decided to merge these four institutes
to form the Institute of Advanced Medical Sciences, in order to facilitate
multi-disciplinal and trans-omic studies for advanced medical sciences. The Institutewas established on 1st April 2016, and currently hosts 17 laboratories.
Supported by the Joint Usage and Joint Research Programs of the MEXT, Japan, theInstitute of Advanced Medical Sciences, Tokushima University, calls for theapplications for the joint usage and/or joint research as follows. The proposed expenses
should be used within the fiscal year 2017, which begins on 1st April 2017 and ends on
31st March 2017.
(A) Joint Usage
State-of-art research facility of our Institute is open for the support of the research in thefollowing 4 aspects. Up to 1,000,000 Japanese yens per successful proposal per fiscal
year will be supported for the following experiments.

A-1. Next-generation sequencing and microarray analyses (organized by Professor
Toyomasa Katagiri)
Representative costs are 778,000 yens per sample for whole human genome sequencing(Illumina Hiseq1500), 384,000 yens per lane (6 samples) for whole human exon
analysis (Illumina Hiseq1500), 235,000 yens per lane (6 samples) for whole humanRNA-seq analysis (Illumina Hiseq1500), 205,000 yens per lane (15 samples) forRNA-seq expression analysis (Illumina Hiseq1500), and 60,000 yens per sample for
Affymetrix human microarray analysis. NGS analysis may be carried out for hereditarydiseasesamples.

Pleasecontact

Professor

Katagiri

fordetails(tkatagi@genome.tokushima-u.ac.jp). Human sample analysis requires the priorapproval from appropriate intramural committee of the applicant’s affiliatedorganization.
A-2. Epigenetic analysis (organized by Professor Makoto Tachibana)
Chromatin extraction, bisulfite sequencing, and chromatin immunoprecipitation with orwithout denaturation. Genome-wide epigenetic analysis using next generation
sequencers may also be supported. Please contact Professors Tachibana and Katagiri fordetails (mtachiba@tokushima-u.ac.jp, tkatagi@genome.tokushima-u.ac.jp).
A-3. Proteome analysis (organized by Professor Hidetaka Kosako)
Mass spectrometry (EASY-nLC 1200 connected to Q Exactive Plus, Thermo Fisher
Scientific) and 2D-DIGE (two-dimensional difference gel electrophoresis, GEHealthcare). Mascot, Proteome Discoverer, Scaffold, SIEVE, Pinpoint, and BioPharma
Finder can be used for data analysis. Representative costs are 15,000 yen per sample for
identification of proteins from gel fragments after 1D or 2D electrophoresis, 20,000 to30,000 yen per sample for identification and quantitation of PTMs such as
phosphorylation and ubiquitination of proteins, 15,000 to 30,000 yen per sample for
global identification and quantitation (by TMT, SILAC, and PRM) of proteins andphosphorylation sites in complex samples such as cell lysates, and 15,000 yen per
sample for 2D-DIGE analysis. Please contact Professor Kosako for details(kosako@tokushima-u.ac.jp). Human sample analysis requires the prior approval fromappropriate intramural committee.

A-4. Genome editing of laboratory mice (organized by Professor Tatsuya Takemoto)Generation of genetically modified mice by an improved zygote electroporation
technology of CRISPR/Cas9-mediated genome editing. Costs are 400,000 yen forgenerating a gene-disrupted mouse and 600,000 yen for a mouse carrying knock-in or
point mutation. Costs for mouse breeding and shipping are also required. Please contact
Professor Takemoto for details (takemoto.tatsuya@tokushima-u.ac.jp).
(B) Joint Research
The Institute welcomes the proposal for collaborative joint research with the following
laboratories. In general, up to 500,000 Japanese yens per proposal per fiscal year will besupported to a successful research proposal. Those who need higher amount of thebudget can make a special request up to 1,000,000 Japanese yens per proposal per fiscalyear, by clarifying the special reason.
B-1. Division of Genome Medicine
Professor Toyomasa Katagiri, tkatagi@genome.tokushima-u.ac.jp
B-2. Division of Epigenome Dynamics
Professor Makoto Tachibana, mtachiba@tokushima-u.ac.jp
B-3. Division of Protein Expression
Professor Yasuo Shinohara, yshinoha@genome.tokushima-u.ac.jp
B-4. Division of Disease Proteomics
Professor Hisaaki Taniguchi, hisatan@tokushima-u.ac.jp
B-5. Division of Cell Signaling
Professor Hidetaka Kosako, kosako@tokushima-u.ac.jp
B-6. Division of Embryology
Professor Tatsuya Takemoto, takemoto.tatsuya@tokushima-u.ac.jp
B-7. Division of Experimental Immunology
Professor Yousuke Takahama, takahama@genome.tokushima-u.ac.jp
B-8. Division of Molecular Biology
Professor Seiichi Oyadomari, oyadomar@genome.tokushima-u.ac.jp
B-9. Division of Inflammation Biology
Professor Tatsuya Saitoh, saitohtatsuya@tokushima-u.ac.jp
B-10. Division of Enzyme Pathophysiology

Professor Kiyoshi Fukui, kiyo.fukui@tokushima-u.ac.jp
B-11. Division of Molecular Neurobiology
Professor Suehiro Sakaguchi, sakaguchi@tokushima-u.ac.jp
B-12. Division of Molecular Immunology
Professor Mitsuru Matsumoto, mitsuru@tokushima-u.ac.jp
B-13. Division of Molecular Medicine
Professor Yoshiyuki Minegishi, yminegishi@genome.tokushima-u.ac.jp
B-14. Division of Immune Regulation
Professor Taku Okazaki, tokazaki@genome.tokushima-u.ac.jp
B-15. Division of Pathology and Metabolome Research for Infectious Disease and HostDefense Professor Hiroshi Kido, kido@tokushima-u.ac.jp
B-16. Division of Molecular Endocrinology
Professor Seiji Fukumoto, fukumoto.seiji.1@tokushima-u.ac.jp
B-17. Division of Diabetes Therapeutics and Research
Professor Munehide Matsuhisa, matuhisa@tokushima-u.ac.jp
Eligibility
A researcher affiliated with a university or a public research institute, or a researcher
approved by the Director of our Institute. The approval from the affiliated organizationis required. The Institute of Advanced Medical Sciences does not discriminate on thebasis of nationality, race, religion, disability, gender, marital status, sexual orientation,age, or any other illegal or unfair basis.
Application
Please convert the complete application that contains the following items to a single
PDF document within two pages, and send as an e-mail attachment to<kyodo@tokushima-u.ac.jp> with the subject line: “Application for Joint Usage Joint
Research Programs”. Application deadline is 28th February 2017.
- Personal information: Full name, Degree(s), Title, Organization, Mailing address,
Phone number, E-mail address, and Country of citizenship- Three names of your references- Representative research records, chiefly within the last five years- Proposed research: Title, Aspect (choose one from Joint Usage A1-A4 and Joint

Research B1-B17), Contact researcher at out Institute, Research aims, Methods,Necessities, Outcomes, Budget, and Research duration
Schedule
Deadline of the application is 28th February 2017. Successful applicants will be notified
before the end of March 2017. Approved joint usage and/or joint research should beperformed between 1st April 2017 and 31st March 2018.
Obligations
Successfully selected applicants must send us a brief report of their research activities
and findings within two pages. The report should include the publication of the papers
and the presentations at scientific meetings. A single PDF document that contains thereport has to be sent to us via e-mail by 31st March 2018.
When the selected applicant is reporting the findings out of the Joint Usage and Joint
Research Programs in an academic paper, she/he must state clearly in that paper that theresearch was carried out under the support of The Joint Usage and Joint Research
Programs, the Institute of Advanced Medical Sciences, Tokushima University. A copyof that paper must be sent to us.
Contact
Administration OfficeInstitute of Advanced Medical Sciences, Tokushima University
3-18-15 Kuramoto, Tokushima 770-8503, Japan
Telephone: +81-88-633-9420
E-mail: kyodo@tokushima-u.ac.jp