PIPETBOY acu 2 Operating instructions

466079_V19

Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
Description

ModelsPIPETBOY acu 2155000, 155015, 155016, 155017, 155018, 155019, 155022,
155023, 155024

Accessories

153210, 153211, 153214, 153216, 155066

comply with:
International

Scope

UN 38.3

Lithium battery testing requirements

EU Directives

Scope2014/35/EU

Low voltage directive (LVD)

20.04.2016

2014/30/EU

Electromagnetic compatibility (EMC)

20.04.2016

2012/19/EC

Waste electrical and electronic equipment (WEEE)

14.02.2014

2011/65/EC

Restriction of hazardous substances (RoHS)

03.01.2013

2006/66/EC

Battery directive

26.09.2008

EU Regulations

Scope

1907/2006

Registration, evaluation, authorisation andrestriction of chemicals (REACH)

01.06.2007

2019/1782

External power supply efficiency

01.04.2020

1103/2010

Capacity labelling of portable batteries

30.11.2010

Date effective

Date effective

EU Standards

Scope

EN 9001:2015

Quality Management

EN 61010-1:2020

Safety general laboratory equipment

EN 61326-1:2013

Electromagnetic compatibility laboratory equipment

EN 60950-1:2013

Safety information technology equipment

EN 62368-1:2021

Safety information technology equipment

EN 62133-2:2017

Batteries containing non-acid electrolytes

i/iii

INTEGRA Biosciences AG

PIPETBOY acu 2 – Declaration of conformity
GBR Regulations

Scope

Date effective

S.I. 2016/1101

Electrical equipment safety

08.12.2016

S.I. 2016/1091

Electromagnetic compatibility (EMC)

08.12.2016

S.I. 2008/2164

Batteries and accumulators regulations

26.09.2008

S.I. 2013/3113

Waste electrical and electronic equipment (WEEE)

01.01.2019

S.I. 2012/3032

Restriction of hazardous substances (RoHS)

02.01.2013

GBR Standards

Scope

BS 61010-1:2010

Safety general laboratory equipment

BS 62368-1:2020

Safety information technology equipment

BS 63000:2018

Restriction of hazardous substances (RoHS)

USA Regulations

Scope

47 CFR Part 15 (FCC)

Electromagnetic compatibility (EMC)

10 CFR Part 430

External power supply efficiency (CEC VI)

Date effective

17 CFR Parts 240 & 249bDodd frank “Conflict minerals”
27 CCR Parts 2510227001

Proposition 65: The safe drinking water andtoxic enforcement act

20 CCR Parts 1601-1608 CEC BCS, Battery charging efficiency

01.01.2017

TSCA 40 CFR Part 751 Toxic substances control act
USA Standards

Scope

UL 61010-1:2012

Safety general laboratory equipment

CAN Standards

Scope

CSA-C22.2
No. 61010-1

Safety general laboratory equipment

www.integra-biosciences.com

ii/iiiPIPETBOY acu 2 – Declaration of conformity
CHN Regulations

Scope

AQSIQ Order 5 /2001(CCC) safety and EMC requirements forelectrical equipment

Date effective
01.08.2003

Order 32/2016

Restriction of hazardous substances (RoHS)

01.07.2016

CHN Standards

Scope

GB4943.1-2011

Information technology equipment safety

GB9254-2008

Information technology equipment radio
disturbance

GB17625.1-2012

EMC limits for harmonic current emissions

GB31241-2014

Safety for Lithium-ion batteries

SJ/T 11364-2014

Restriction of hazardous substances (RoHS)

JPN Regulations

Scope

PSE (Denan) Law

Electrical appliance and material safety law

Date effective
01.01.2014

EAC Технический регламент Таможенного союзаТР ТС 004/2011О безопасности низковольтного оборудованияТР ТС 020/2011Электромагнитная совместимость технических средств

Zizers, April 11, 2022
Urs HartmannCEOiii/iii

Daniel Bächi
Head of Corporate Quality

INTEGRA Biosciences AG

PIPETBOY acu 2 - Operating instructions V19

Table of Contents
1
2
3
4
5
6
7

Introduction ................................................................................................................ 6
Description of the device ............................................................................................ 8
Installation ...................................................................................................................9
Operation ................................................................................................................. 10
Maintenance............................................................................................................. 13
Technical Data ......................................................................................................... 14
Accessories .............................................................................................................. 16

Imprint© 2023 INTEGRA Biosciences AG
This operating instruction manual applies to serial number 1350 000 or higher of
PIPETBOY acu 2 / PIPETGIRL.
Manufacturer and customer service
Your local INTEGRA Biosciences representative, further information, and operating
instructions in other languages can be found at www.integra-biosciences.com or areavailable on request info@integra-biosciences.com.
INTEGRA Biosciences AG
Tardisstrasse 201
CH-7205 Zizers, Switzerland
T +41 81 286 95 30

INTEGRA Biosciences Corp.
22 Friars Drive
Hudson, NH 03051, USA
T +1 603 578 5800

INTEGRA Biosciences SAS
8 avenue du Fief
95310 Saint Ouen l'Aumône, FR
T +33 1 34 30 76 76

INTEGRA Biosciences Deutschland GmbHAn der Amtmannsmühle 135444 Biebertal, DE
T +49 6409 81 999 15

INTEGRA Biosciences Ltd
2 Rivermead Business Park
Thatcham, Berks, RG19 4EP, UK
T +44 1635 797 00

INTEGRA Biosciences Nordic ApS
Vallensbækvej 22A 3TV
Brøndby 2605, DK
T +45 3173 5373

INTEGRA Biosciences KK
Higashikanda 1-5-6, Chiyoda-ku
Tokyo, 101-0031, JP
T +813 5962 4936

INTEGRA Biosciences (Shanghai) Co., Ltd.
Room 1110, No. 515 Huanke Road
Shanghai 201315, CN
T +86 21 5844 7203

www.integra-biosciences.com

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PIPETBOY acu 2 - Operating instructions V19

1

Introduction

1.1

Intended use

Introduction

PIPETBOY acu 2 is a pipette controller designed for aspirating and dispensing aqueous
solutions with plastic or glass pipettes of 1 to 100 ml volumes. It is intended formeasurement, control and laboratory use. Any use of this instrument in a medical or IVD
setting is under the sole responsibility of the user.
PIPETGIRL is a special model of PIPETBOY acu 2 and provides the same functions as
PIPETBOY acu 2.

1.2

Safety notes

1) Do not use or charge PIPETBOY acu 2 in an atmosphere with danger of explosion.
Also, do not pipette highly flammable liquids such as acetone or ether.
2) When handling dangerous substances, comply with the material safety data sheet(MSDS) and with all safety guidelines such as the use of protective clothing and safety
goggles. Never point a pipette in anyone’s direction.
3) Avoid pipetting of liquids whose vapours could attack the materials PA (polyamide),
POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrile-rubber), CR (chloroprene),silicone. Corrosive vapours could also damage metallic parts inside the device.
4) Prolonged exposure of PIPETBOY acu 2 to UV-light can cause discolouration and/or
yellowing of the plastic housing. However, this will not affect the performance of thedevice in any way.
5) Only use the original Li-ion battery (part no. 155066) and an original
INTEGRA Biosciences mains adapter and protect it from moisture, otherwise
PIPETBOY acu 2 might be damaged.
6) Old Li-ion batteries may cause a safety risk. We recommend to replace the battery after
3 years of use. Also replace the battery if the charging intervals are unusually short orif the charging takes much longer than usual (4 hours or more). These are indicatorsthat the battery has reached the end of its life-cycle.
If a lithium battery is never deep discharged and is always stored and operated in therecommended temperature range and stored at 40-80% charge level during longstandby periods, it may live much longer than 3 years. If it shows no signs of physicaldamage or change, see 5.1, it is a strong indication that you may continue to use thebattery.
7) Li-ion technology bears the risk of thermal runaway and cell rupture if the battery wasdamaged. Do not expose the battery to heat (> 60°C) and avoid mechanical stress.
Batteries which were subject to deep discharges may develop internal short circuits,
leading to an increased self-discharge rate and heating during battery charging. This
may also result in thermal runaway and cell rupture.

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INTEGRA Biosciences AG

PIPETBOY acu 2 - Operating instructions V19

Introduction

8) To extend the battery life-cycle, it is recommended to charge the battery every 2months if the pipette controller is not used regularly. If the pipette controller is not used
for more than 6 months, remove the battery from the instrument.
Regardless of the listed safety notes, additionally applicable regulations and guidelines oftrade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

www.integra-biosciences.com

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PIPETBOY acu 2 - Operating instructions V19

2

Description of the device

2.1

Scope of delivery

Description of the device• PIPETBOY acu 2 device• 1 rechargeable Li-ion battery• Mains adapter• Wall mount• Hydrophobic sterile filter 0.45 µm (spare)• Quick Start Guide

2.2

Overview of PIPETBOY acu 2
4

1 Aspiration button
3

7
6

1
2

b
c
d
e

3 Thumb wheel to set maximum speed
4 Battery charge indicator
5 Socket for mains adapter cable
6 Handle5

7a

2 Dispensing button

7 Sterile module complete (=nose piece)

7 Sterile module complete (=nose piece)7aO-ring7b

Housing of sterile module, upper part

7c

Filter rubber

7d

Hydrophobic filter

7e

Pipette mount

7f

Housing of sterile module, lower partf

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INTEGRA Biosciences AG

PIPETBOY acu 2 - Operating instructions V19

3

Installation

3.1

Charging the battery

Installation

A full charge takes 3.5 hours. Before the first use, PIPETBOY acu 2 should be charged
until the battery charge indicator (4) turns green, showing that the battery is full.
When the battery charge indicator (4) starts flashing red, PIPETBOY acu 2 can be used
for around 100 pipetting cycles before shutting down. It should thus be recharged
immediately.
PIPETBOY acu 2 has an integrated protection: it will not overcharge even if it is connected
to power for indefinite time. To avoid unnecessary power consumption, it is recommended
to unplug the power supply when the charge indicator is green. PIPETBOY acu 2 can beused while it is being charged.
The battery charge indicator provides various information:
Battery charge indicator

Battery status and information

Flashes red
Is red and power supply is connected
Is green and power supply is connectedFlashes alternately red and green

Battery is low. Charging is needed.
Battery is being charged.
Battery is fully charged.
Battery error. Check if the correct type ofbattery with correct polarity (+/-) and/or
power supply are used.

3.2

Replacing the batterya1) Move the lid of the battery compartment upwards andremove it (a).
2) Replace the old battery with an original
INTEGRA Biosciences rechargeable battery (see “7Accessories” on page 16) and make sure that it isinserted with the correct polarity (+/-).
3) Close the battery compartment with the lid (a).

3.3

Mounting of the wall mount

The wall mount serves to park PIPETBOY acu 2.
To mount the wall mount, remove the protective foil from the adhesive tape at the back of
the holder. Hold it with the sign <up> facing upwards and press it to the desired place.
Make sure that the surface onto which the wall mount is mounted is smooth, clean andgrease-free. Wait 24 hours before using the wall mount for the first time. Alternatively thewall mount can be fixed with the included screws.

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PIPETBOY acu 2 - Operating instructions V19

4

Operation

4.1

Inserting the pipettea)b)Operation

The silicone pipette mount (7e) has a special conicalchannel to guarantee a firm and leak-proof grip of thepipette independently of its diameter.
Disassemble the nose piece (see “5.1 Cleaning andservicing” on page 13) and orient the pipette mount:a) with the large opening facing down for pipettes > 2 ml
(factory setting), orb) with the small opening facing down for pipettes < 2 ml.

WARNING
Do not insert pipettes with force into PIPETBOY acu 2, because they can breakand cause injury, particularly thin pipettes made of glass.

4.2

PipettingPress the aspiration button (1) to fill the pipette and the dispensing button (2) to empty it.
The aspiration and dispensing speed can be controlled in two manners:• Fine speed adjustment by varying the finger pressure on the buttons (1, 2).
• Step-less presetting of the maximum pump speed by turning the thumb wheel (3) tooptimally match the pipette volume (turning to the left = slower pump speed, for small
pipettes; to the right = faster, for large pipettes).
To empty the pipette by gravity force, press the dispensing button only slightly in order toavoid reaching the trigger point where the pump starts running. Gravity dispensing is used
for “to deliver” (TD) pipettes that are not of the “blow-out” type (blow-out pipettes have twothin rings or a frosted band around the neck).
PIPETBOY acu 2 is featured with a “TURBO” mode. Plug the mains adapter cable into thepipette controller and turn the thumb wheel completely to the right for maximal speed.

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INTEGRA Biosciences AG

Pipette is damaged or not fully inserted in
the nose piece (7).

Pipette drips(leak in the system).

Reinsert a new pipette and push it all the way into
the nose piece. Make sure that the pipette mountorientation is correct (see section 4.1).
Replace the pipette mount (#151 020).

RemedyThe inside of the pipette mount (7e) isdamaged resulting in insufficient sealing of
the pipette neck.
The filter rubber (7c) or the filter (7d) in the Replace the filter rubber (#153225) and/or thenose piece are damaged or missing causing filter (Part No. see “7 Accessories” on page 16).
a leak.
Replace filter.
Reduced aspiration
The filter (7d) is wet or dirty.
efficiency or no liquid
The nose piece (7) is not tight.
Tighten the nose piece, or replace defective parts.
aspiration.
The battery is discharged (battery charge Charge the battery.
indicator flashes red)
The battery is missing.
Insert the battery or connect the instrument to themains adapter.
The battery is defective.
Replace the battery.
The battery is wrongly inserted.
Insert correctly, note polarity (+) and (-).
Reduced operating time The battery is worn.
Replace the battery.
with fully charged battery. Wrong battery type is inserted.
Use only original Li-ion battery (#155 066).
Extremely long charging Wrong mains adapter is used.
Use only original mains adapter (see “7time of battery.
Accessories” on page 16).
Extremely short charging Wrong battery type is inserted.
Use only original Li-ion battery (#155066).
and operating time.

Probable cause

Troubleshooting

Problem

4.3

PIPETBOY acu 2 – Operating instructions V19

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Operation

11

12

The battery is wrongly inserted.
Wrong battery type is inserted.
Wrong mains adapter is used.
The battery is wrongly inserted.
Wrong battery type is inserted.
The battery is missing.

Battery is not charging.

Battery operation notpossible.

Probable cause

ProblemInsert correctly, note polarity (+) and (-).
Use only original Li-ion battery (#155066).
Use only original mains adapter (see section 7).
Insert correctly, note polarity (+) and (-).
Use only original Li-ion battery (#155066).
Insert the battery.

RemedyPIPETBOY acu 2 – Operating instructions V19
Operation

INTEGRA Biosciences AG

PIPETBOY acu 2 - Operating instructions V19

5

Maintenance

Maintenance

After maintenance work, perform a leak test to ascertain correct functioning of
PIPETBOY acu 2: liquid should not leak out of a filled pipette before the dispensing button
is pressed.

5.1

Cleaning and servicing

PIPETBOY acu 2 can be cleaned with a cloth moistened with soapy water or with a 70 %ethanol.
Disassembly of the nose piece:
Unscrew the nose piece (7) from the handle by turning it
counter clockwise. Hold the upper part of nose piece (7b),press the lower part (7f) firmly against the upper part (7b)and turn it counter clockwise (left). The lower part of nose
piece housing (7f) will disengage after about 1/8th of aturn.
Remove the pipette mount (7e), the filter (7d) and thefilter rubber (7c), if required.
It is recommended to change the hydrophobic filter (7d) every three months. Should thefilter get wetted or soiled, it has to be changed immediately. If one side of the filter iscolored, it must face upwards towards PIPETBOY acu 2. Clear (transparent) filters can beinserted in any direction.
Annual inspection: If you operate the battery beyond the recommended 3 year period,
visually check the battery for signs of damage, e.g. discoloration, unexpected stains,shrinking of the tube wrapping.

5.2

Decontamination

The nose piece housing (7b, 7f), the pipette mount (7e) and the filter rubber (7c) can be
autoclaved at 121 °C, 1 bar overpressure for 20 minutes. Silicone may become brittle after
extensive autoclaving. Replace the pipette mount and filter rubber if they are damaged.
If the housing of the PIPETBOY acu 2 have been in contact with biohazardous material,
it must be decontaminated in accordance to good laboratory practice. Do not spray directlyon the instrument but use a lint-free cloth, lightly soaked with a disinfectant and wipe dry
directly after decontamination. Never use acetone or other solvents! Follow the
instructions provided by the disinfectant manufacturer.
The device may be decontaminated with H2O2 gas (maximal concentration 35 %) for 60
minutes.

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PIPETBOY acu 2 - Operating instructions V19
5.3

Technical Data

Equipment disposal
PIPETBOY acu 2 device must not be disposed of with unsorted municipal
waste. Do not dispose of the device in a fire.
PIPETBOY acu 2 contains a Li-ion battery. Do not modify the battery in any
way. Dispose of the PIPETBOY acu 2 device and the battery separately inaccordance with the laws and regulations in your area governing disposal ofdevices containing Li-ion batteries.

In certain regions and countries, e.g. in EU member states, the distributor is obliged to takeback this product free of charge at the end of life. Please contact your local distributor for
more details.

6

Technical Data

6.1

Specifications

Pipetting speed
Battery

Electricity supply
Materials

Dimensions (H x W x D)
WeightAmbient conditions

14

max. 13.5 ml/s (with a 50 ml serological pipette)
Type: rechargeable, Li-ion, min. 500 mAh
Typical charging time: 3.5 hours
Charging cycles: 500–1000 (when charging as indicated)
Running time: at least 5500 cycles of aspiration anddispensing of 25 ml.
Mains adapter input: 100–240 VAC, 50/60 Hz
Device Input: 16–19 VDC, 3.1 W
Housing: PA
Nose piece housing: POM
Pipette mount: Silicone
Filter rubber: Silicone
125 x 130 x 35 mm
195 g
Operation: 5–40°C, max. 80% RH
Storage: -10–50°C, max. 95% RH

INTEGRA Biosciences AG

PIPETBOY acu 2 - Operating instructions V19
6.2

Technical Data

Chemical compatibility

The table below lists PIPETBOY acu 2 parts that come into contact with the aspirated
liquid or its aerosols and vapors, and rates the compatibility of these parts to a few of thechemicals commonly used in laboratories. To determine the compatibility of a component
to a chemical not listed in the table, please consult one of the several tables available on
the internet. Note that the rating refers to soaking in the concentrated chemical; however,more relevant here is the attenuated effect resulting from vapors and the diluted chemical.
It is recommended to test the compatibility of relevant components to a specific chemical
prior to extensive use.
INTEGRA Biosciences does not warrant that the information in the table is accurate orcomplete and that any material is suitable for any purpose.
Chemical compatibility chart
Sodium hydroxide (50%)

Sodium acetate(3M, pH 5.2)

Hydrochloric acid (20%)

Chloroform

Acetone

A

A

B

A

A

A

C

A

C

C

A

C

C

A

A

C

A

A

A

A

A

C

C

A

A

A

A

A

B

A

A

B

C

A

C

A

A

NaCl saturated

A

Isopropyl alcoholC

EthanolC

Acetic acidA

Materials

JAVEL (e.g. NaClO)

A

Parts
Handle

PA

A

C

B

A

A

Nose piece housing

POM

C

C

A

A

A

Pipette mount,
Filter rubber, tubings

Silicone

A

B

A

A

A

Internal parts(e. g. pump)

FPM

A

A

A

A

NBR

A

B

B

CR

A

A

A

Metal

C

C

A

Compatibility ratings:
A = Good: no or minor effects.
B = Fair: moderate effects, not recommended for continuous use.
C = Critical: not recommended, suitability to be determined by test.

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PIPETBOY acu 2 - Operating instructions V19

7

Accessories

Accessories

Accessories
Stand for
PIPETBOY
Wall mount
Mains adapter(100-240 VAC,
50/60 Hz)

Part No.
for a well organized work area, to park PIPETBOY acu 2 withinserted pipettefor holding PIPETBOY acu 2 on the wall
EU version
US/JP version
UK version
AU version

Consumables
Sterile module(nose piece)
complete (7)Sterile module(nose piece)
housing (7b,7f)
Filter 0.45 µm(7d)Filter 0.2 µm(7d)155521153210153211153214153216
Part No.

turquoise for PIPETBOY acu 2 classic and transparent;with filter 0.45 µm, unsterileclassic (grey) for colored PIPETBOY acu 2 and PIPETGIRL;
with filter 0.45 µm, unsterileturquoise, for PIPETBOY acu 2 classic and transparent
classic (grey), for colored PIPETBOY acu 2 and PIPETGIRL

155025155070155230155060blue, sterile/unsterile, for the country specific part numbers,
please refer to our website
red or clear, sterile/unsterile, for the country specific partnumbers, please refer to our website

silicone, for holding pipette in the sterile module (nose piece)
Pipette mount(7e)
Filter rubber (7c) silicone, for holding the filter in the sterile module (nosepiece)
O-ring (7a)Battery
classic (grey)
compartment lid green
red
bluetransparent
purplepink (PIPETGIRL) / Pink Sunrise
Agave
Ocean Dream

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155065151020153225153235155203155160155161155162155163155164155166155167155168INTEGRA Biosciences AG

PIPETBOY acu 2 - Operating instructions V19

Consumables
Battery Li-ion

Accessories

Part No.
for PIPETBOY acu 2 / PIPETGIRL(with serial number >=1350001)

WARNING
Only use the battery Li-ion for PIPETBOY acu 2(serial number >=1350001; LED is white when inactive,
red or green when active)!
The NiMH battery for PIPETBOY acu is not compatible!

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155066serial number

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