受験番号：28IPB001

問１
Insects are broadly divided into Holometabola and Hemimetabola according to the form ofdevelopment. The morphogenesis of Holometabola such as Drosophila has been well studied, while
the morphogenesis of Hemimetabola has been little studied.

However, some of the pests arehemimetabolous insects such as grasshoppers, locusts and cockroaches, and thus the genetic
recombination techniques are requisite to control the hemimetabolous insects.

The genetic

recombination techniques are also requisite when hemimetabolous insects are used as natural enemy
insects.
In one of the techniques of genetic recombination, transgenesis, in which a specificexogenous gene is introduced, the efficiency of incorporation of the exogenous DNA into genomic
DNA is low.

Therefore, a special means is required in order to increase the efficiency.

DNAtransposons are currently spread as the means therefore. DNA transposons are excised from DNA
by transposases and inserted to target sequences in different DNA.
問２
After extraction, a mixture of the pulverized material and the solvent may be subjected tosolid-liquid separation according to a well-known method, and only the extraction solution may berecovered.
An extract containing ergosterol peroxide may be obtained by optionally dehydrating theextraction solution with a dehydrating agent such as anhydrous sodium sulfate and then distilling off
the solvent. The thus obtained extract may be further appropriately fractionated and separated bycolumn chromatography such as liquid chromatography, extraction, fractional precipitation and thelike. The fraction thus having an increased purity may be used instead of the extract.
The neuronal cell activating agent contains a peroxide of ergosterol as an active component.
The content of the active component may vary according to the age and body weight of the subject
taking the agent.

It is preferable that the content is such that 0.1 to 1000 mg/kg, more preferably 1

to 100 mg/kg and most preferably 5 to 50 mg/kg of the peroxide of ergosterol can be taken per day byan adult.

問３<Juvenile blowfish estrogen administration test 1>
An effect on gonads of administration of estrogen to blowfish during a period when gonads
are sexually plastic is examined. The estrogen used was synthetic estradiol and the blowfish used
was Fugu rubripes. Estradiol in the form of an ethanol solution dissolved in ethanol was periodicallyadded to the breeding water. More than one test section was established to which variously different
amounts of estrogen were added. Specifically, 8 sections in total were established including test
section 1 with the estrogen concentration in the breeding water of 1 ppb by weight (1 ng/g); test section
2 with 5 ppb by weight (5 ng/g); test section 3 with 10 ppb by weight (10 ng/g); test section 4 with 25ppb by weight (25 ng/g); test section 5 with 50 ppb by weight (50 ng/g); and test section 6 with 100
ppb by weight (100 ng/g); non-treatment control section 1 with no addition; and control section 2 withaddition of ethanol only (estradiol concentration: 0 ppb by weight). The amounts of ethanol in all
sections were the same.
問４
What Is Claimed Is:1.

A method for inducing cataract, comprising:a damaging step for damaging a DNA of a collected lens or a lens of a non-human organism.

3.

The method for inducing cataract according to claim 1 or 2, wherein:
the damaging step is a step for damaging a DNA by applying a stimulus with a predetermined

intensity to the collected lens or the lens of the non-human organism, andthe intensity of the stimulus is in an extent that only a part of a cross-section of the lens is
opacified after application of the stimulus.
4.

The method for inducing cataract according to any one of claims 1 to 3, wherein thedamaging step comprises (i) and/or (ii) indicated below:(i) delivering ultraviolet or radiation to the collected lens or the lens of the non-humanorganism;(ii) bringing the collected lens or the lens of the non-human organism into contact with asubstance inducing DNA damage.
5.

A cataract model organism obtained by the method according to any one of claims 1 to 4.