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KOT – Tubular C ils

USA – Biotech USA – info@biotechusa.us – SWEDEN – Biotech AB – info@biotech.se – INDIA – Biotech Pvt Ltd – tech@biotechltd.in

eQuant

vators in Chemical Analysis

KOT – Tubular Coils™

KOT - Tubular Coils

KNITTED OPEN TUBULAR REACTION DELAY AND MIXING COILS• Tortous flow path of optimal flow dynamic design made fromtotally inert PTFE• Minimised axial dispersion combined with efficient radial mixing
KNITTED OPEN TUBULAR
REACTION DELAY AND MIXING COILS• Available individually in different lengths and inner diameters Tortous flow path of optimal flow dynamic design made from totally inert PTFE Minimised axial dispersion combined with efficient radial mixing
 Available individually in different lengths and inner diameters

Preserved ChromatographicPreserved
Peak ShapeChromatographic Peak ShapeThe Biotech knitted open tubular(KOT)
reactors
othereventa certain
time
cantake place.
The SeQuantknittedopenaretubular (KOT)
reactors
are thatmaderequires
from tubing
which has
beenknitted
made from tubing which has
beenknitted
into
a tortuDifferent
reaction
accomplishedbyinto a tortuous path to force
the fast moving
centredelayof theorliquid
streamtimes
to mixare
radially
with theous path to force the fast moving
centreboundaryof the liquidchanging
the innerand lengthof theslower moving
liquid layer, thereby
minimizing
the diameteraxial dispersion.
The shapeof aKOT,
stream to mix radially withchromatographic
the slower moving
bound-the reactor
peak enteringis thusefficiently.
ensurea stabletaking
thepreserved
flow ratevery
throughtheToKOT
into
considerary liquid layer, thereby minimizing
theaxialthe
disperradial mixing
withinKOT, a linear flowrate of 10 cm/s or more is recommended. The KOTation.
sion. The shape of a chromatographic
peaktheentering
reactor is usuallymost optimal delay element for use in analytical flow systems, and istypicallyinserted in theflow path to create athe reactor is thus preserved
very efficiently.
To ensure
Recommended Minimum Flow Ratedelayso athata reaction
other event thata stable radial mixing within
theline,KOT,
linearflow orrate
Linear Flow
Volumetric Flowrequires a certain
time reactor
can take place. Different IDof 10 cm/s or more is recommended.
The KOT
mm
cm/s
mL/mindelayorreactiontimes
areaccomplishedby
is usually the most optimal delay element for use in
0.25
100.29the inner
diameterand length of theanalytical flow systems, andchanging
is typicallyinsertedin the0.50
101.2
the aflowrate throughthe KOT into 0.75
102.7
flow path to create a delayKOT,
line,taking
so thatreactionorconsideration.

The KOT Reactor is the Most Optimal Delay
Element for
Use inAnalytical
Flow Systems
The KOT Reactor
is theMostOptimal
Delay

Element for Use in Analytical Flow Systems
Separation Column

Split

KOTFraction
Collector

MS

Reagent Supply
KOT

Separation Column

Reagent Supply
Tee

Separation Column

Split

MSDetector

KOTColumn
A KOT
applied as a delay coil allowing the result fromSeparation
Typicaluse of a KOT as a mixer andKOT
reactor in HPLC
Fraction
the MS to control the fraction collector
post-column reaction detection
Collector
TeeDetector

Example Application Areas

SeQuant KOT reactors can be applied as a delay line in chromatographic separations. One
example being the parallel coupling of a MS detector and a fraction collector in preparative
A KOT applied as a delay coilseparations
allowing theresult
Typicalof a KOT
as a KOT
mixerand reactor
in HPLC(seeleft figure above). The delay
timeuseinducedby theallows
the detector
tofrom the MS to control the fraction collector
reaktion reaction detection
analyse the eluate and make intelligent decisionspost-columnof when a new
fraction is going to be initiated,without loss of chromatographic efficiency.
The SeQuant KOT reactors can also be used in post-column reaction detection in HPLC (seeright figure above), using both room temperature and heated chemistries. In this set-up theExample Application Areas
Biotech KOT reactors can be applied as a delay line inchromatographic separations. One example being theparallel coupling of a MS detector and a fraction collector in preparative separations (see left figure above).
The delay time induced by the KOT allows the detector
to analyse the eluate and make intelligent decisions ofwhen a new fraction is going to be initiated, without
loss of chromatographic efficiency.

The Biotech KOT reactors can also be used in postcolumn reaction detection in HPLC (see right figureabove), using both room temperature and heatedchemistries. In this set-up the KOT also ensure thorough mixing between the column effluent and theadded reagents. Typical reactions are the productionof fluorescent products from reactions between eluted
compounds and reagents without native fluorescence.

Normal instrumentation of a UV photoreactor in an LC instrument.

Time (min)TCAA (16ppb)

DBAA (15ppb)

DCAA (18ppb)

MBAA (14ppb)

MCAA (14ppb)

Peak Height (mV)

Typical PC-IC Chromatogram

Photooxidation of phenylalanine

P/N
3000-123

KOT - Tubular coils
Knitted Open Tubular (KOT) Reactor, PTFE,  1 m, 0.25 mm ID  

3000-125

Knitted Open Tubular (KOT) Reactor, PTFE,  1 m, 0.5 mm ID  

3000-128

Knitted Open Tubular (KOT) Reactor, PTFE,  1 m, 0.75 mm ID  

3000-223

Knitted Open Tubular (KOT) Reactor, PTFE,  2 m, 0.25 mm ID  

3000-225

Knitted Open Tubular (KOT) Reactor, PTFE,  2 m, 0.5 mm ID  

3000-228

Knitted Open Tubular (KOT) Reactor, PTFE,  2 m, 0.75 mm ID  

3000-423

Knitted Open Tubular (KOT) Reactor, PTFE,  4 m, 0.25 mm ID  

3000-425

Knitted Open Tubular (KOT) Reactor, PTFE,  4 m, 0.5 mm ID  

3000-428

Knitted Open Tubular (KOT) Reactor, PTFE,  4 m, 0.75 mm ID  

3000-133

Knitted Open Tubular (KOT) Reactor, PTFE,  10 m, 0.25 mm ID

3000-135

Knitted Open Tubular (KOT) Reactor, PTFE,  10 m, 0.5 mm ID

3000-138

Knitted Open Tubular (KOT) Reactor, PTFE,  10 m, 0.75 mm ID

3000-525

Knitted Open Tubular (KOT) Reactor, PTFE,  5 m, 0.5 mm ID  Please inform me about prices and delivery terms

Zip CodeNAMECountry

Company / InstituteTelephone

AdDress

E-mailBiotech AB. P.O. Box 133, SE-439 23 Onsala, Sweden.
Telephone +46 (0)300-56 91 80. Fax +46 (0)300-56 91 81
info@biotech.se – www.biotech-sweden.com
USA – Biotech USA – info@biotechusa.us – SWEDEN – Biotech AB – info@biotech.se – INDIA – Biotech Pvt Ltd – tech@biotechltd.in

www.sommar.se

Most dimensions also available in black PTFE - intended for light senstive compounds
Other KOTs might be custom manufactured, please contact Biotech AB for further details