Biodynamics Laboratory Inc.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------MATERIAL SAFETY DATA SHEET
Date Updated: 07/21/2021--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 1 > Product and Company Information--------------------------------------------------------------------------------------------------------------------------------------------------------------------Product NameLong ssDNA Gel Extraction Kit for 3kb(DS640)(Code#)Long ssDNA Gel Extraction Kit for 10kb (DS650)
Component
Crystal Violet Solution
Gel-Dissolving Buffer
Company
Address
Phone
Fax

BioDynamics Laboratory Inc.
9-7 Hongo 2-Chome, Bunkyo-Ku, Tokyo 113-0033 JAPAN+81-3-5803-9983+81-3-5684-6270

Research use only--------------------------------------------------------------------------------------------------------------------------------------------------------------------<2 > Hazards Identification---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Classification
Eye irritation (Category 2), H319
Carcinogenicity (Category 2), H351
Long-term (chronic) aquatic hazard (Category 2), H411
For the full text of the H-Statements mentioned in this Section, see Section 16.
Symbol:Signal word: Warning
Hazard statement(s):H351 Suspected of causing cancer.
Precautionary statement(s):P201 Obtain special instructions before use.
P202 Do not handle until all safety precautions have been read and understood.
P308 + P313 IF exposed or concerned: Get medical advice/ attention.
Supplemental Hazard Statements:
none
Other hazards:
This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very
persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.

Component: Gel-Dissolving Buffer
Classification
Classification of the substance or mixture-1ver. 1.0

Acute toxicity, Oral (Category 4), H302
Acute toxicity, Inhalation (Category 4), H332
Acute toxicity, Dermal (Category 4), H312
Skin corrosion (Sub-category 1C), H314
Serious eye damage (Category 1), H318
Long-term (chronic) aquatic hazard (Category 3), H412
For the full text of the H-Statements mentioned in this Section, see Section 16.
Symbol:Signal word: Danger
Hazard statement(s):H302 + H312 + H332 Harmful if swallowed, in contact with skin or if inhaled.
H314 Causes severe skin burns and eye damage.
H412 Harmful to aquatic life with long lasting effects.
Precautionary statement(s):P273
Avoid release to the environment.
P280
Wear protective gloves/ protective clothing/ eye protection/ faceprotection.
P301 + P312 + P330
IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth.
P303 + P361 + P353
IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P312
IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a
POISON CENTER/doctor if you feel unwell.
P305 + P351 + P338 +P310
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if
present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor.
Supplemental Hazard information (EU) EUH032 Contact with acids liberates very toxic gas.
Other hazards:
This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very
persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------<3 > Composition/Information on Ingredients---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
MixtureIngredient nameCrystal Violet

CAS No.
548-62-9

Content (%)
0.4%

Component: Gel-Dissolving Buffer
MixtureIngredient name
Guanidinium
thiocyanate

CAS No.
593-84-0

Content (%)>40%--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 4 > First Aid Measures---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
General advice:Show this material safety data sheet to the doctor in attendance. If inhaled After inhalation: fresh air. Call in physician.
-2ver. 1.0

In case of skin contact:
In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with water/ shower. Consult a physician.
In case of eye contact:
After eye contact: rinse out with plenty of water. Call in ophthalmologist. Remove contact lenses.
If swallowed:
After swallowing: immediately make victim drink water (two glasses at most). Consult a physician.
Most important symptoms and effects, both acute and delayed:
The most important known symptoms and effects are described in the labelling (see section 2) and/or in section 11
Indication of any immediate medical attention and special treatment needed:
No data available

Component: Gel-Dissolving Buffer
General advice:Consult a physician. Show this safety data sheet to the doctor in attendance.
If inhaled:
If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
In case of skin contact:
Take off contaminated clothing and shoes immediately. Wash off with soap and plenty of water. Consult a physician.
In case of eye contact:
Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
If swallowed:
Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
Most important symptoms and effects, both acute and delayed:
The most important known symptoms and effects are described in the labelling (see section 2) and/or in section 11
Indication of any immediate medical attention and special treatment needed:
No data vailable--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 5 > Fire Fighting Measures---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Extinguishing media
Suitable extinguishing media:
Use extinguishing measures that are appropriate to local circumstances and the surrounding environment.
Unsuitable extinguishing media:
For this substance/mixture no limitations of extinguishing agents are given.
Special hazards arising from the substance or mixture:
Carbon oxides
Nitrogen oxides (NOx)Hydrogen chloride gas
Not combustible.
Ambient fire may liberate hazardous vapours.
Advice for firefighters:
Stay in danger area only with self-contained breathing apparatus. Prevent skin contact by keeping a safe distance or by wearing
suitable protective clothing.
Further information:
Suppress (knock down) gases/vapors/mists with a water spray jet. Prevent fire extinguishing water from contaminating surface
water or the ground water system.

Component: Gel-Dissolving Buffer
Extinguishing media
Suitable extinguishing media:
Dry powder Dry sandSpecial hazards arising from the substance or mixture:
Carbon oxides, Nitrogen oxides (NOx), Sulphur oxides, Hydrogen cyanide (hydrocyanic acid), Ammonia
Carbon oxides, Nitrogen oxides (NOx), Sulphur oxides
Combustible.
-3ver. 1.0

Advice for firefighters:
Wear self-contained breathing apparatus for firefighting if necessary.
Further information:
No data available--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 6 > Accidental Release Measures---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Personal precautions, protective equipment and emergency procedures:
Advice for non-emergency personnel: Do not breathe vapors, aerosols. Avoid substance contact. Ensure adequate ventilation.
Evacuate the danger area, observe emergency procedures, consult an expert.
For personal protection see section 8.
Environmental precautions:
Do not let product enter drains.
Methods and materials for containment and cleaning up:
Cover drains. Collect, bind, and pump off spills. Observe possible material restrictions (see sections 7 and 10). Take up withliquid-absorbent material (e.g. Chemizorb® ). Dispose of properly. Clean up affected area.
Reference to other sections:
For disposal see section 13.

Component: Gel-Dissolving Buffer
Personal precautions, protective equipment and emergency procedures:
Wear respiratory protection. Avoid breathing vapours, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.
For personal protection see section 8.
Environmental precautions:
Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into the environment must be avoided.
Methods and materials for containment and cleaning up:
Soak up with inert absorbent material and dispose of as hazardous waste. Do not flush with water. Keep in suitable, closed
containers for disposal.
Reference to other sections:
For disposal see section 13.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 7 > Handling and Storage---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Precautions for safe handling
Advice on safe handling:
Work under hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols.
Hygiene measures:
Immediately change contaminated clothing. Apply preventive skin protection. Wash hands and face after working with
substance.
For precautions see section 2.
Conditions for safe storage, including any incompatibilities
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept
upright to prevent leakage.
Recommended storage temperature: Room TemperatureSpecific end use(s)Apart from the uses mentioned in section 1 no other specific uses are stipulated

Component: Gel-Dissolving Buffer
Precautions for safe handling
Avoid contact with skin and eyes. Avoid inhalation of vapour or mist.
For precautions see section 2.
Conditions for safe storage, including any incompatibilities
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept
upright to prevent leakage.
Recommended storage temperature: Room Temperature-4ver. 1.0

Specific end use(s)Apart from the uses mentioned in section 1 no other specific uses are stipulated--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 8 > Exposure Controls / PPE---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Control parameters
Ingredients with workplace control parameters
Exposure controls
Personal protective equipment
Eye/face protection:
Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN
166(EU). Safety glasses
Skin protection:required
Body Protection:
protective clothing
Respiratory protection:required when vapours/aerosols are generated. Our recommendations on filtering respiratory protection are based on thefollowing standards: DIN EN 143, DIN 14387 and other accompanying standards relating to the used respiratory protection
system. Recommended Filter type: Filter type ABEKThe entrepeneur has to ensure that maintenance, cleaning and testing of respiratory protective devices are carried out according
to the instructions of the producer.
These measures have to be properly documented.
Control of environmental exposure:
Do not let product enter drains.

Component: Gel-Dissolving Buffer
Control parameters
Components with workplace control parameters
Exposure controls
Appropriate engineering controls
Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at the end of workday.
Personal protective equipment
Eye/face protection:
Tightly fitting safety goggles. Faceshield (8-inch minimum). Use equipment for eye protection tested and approved underappropriate government standards such as NIOSH (US) or EN 166(EU).
Skin protection:
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outersurface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws
and good laboratory practices. Wash and dry hands. The selected protective gloves have to satisfy the specifications ofRegulation (EU) 2016/425 and the standard EN 374 derived from it.
Full contact
Material: Nitrile rubber
Minimum layer thickness: 0,11 mm
Break through time: 480 min
Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)
Splash contact
Material: Nitrile rubber
Minimum layer thickness: 0,11 mm
Break through time: 480 min
Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)
data source: KCL GmbH, D-36124 Eichenzell, phone +49 (0)6659 87300, e-mail sales@kcl.de, test method: EN374
If used in solution, or mixed with other substances, and under conditions which differ from EN 374, contact the supplier of theCE approved gloves. This recommendation is advisory only and must be evaluated by an industrial hygienist and safety officer
familiar with the specific situation of anticipated use by our customers. It should not be construed as offering an approval forany specific use scenario.
-5ver. 1.0

Body Protection:
Complete suit protecting against chemicals, The type of protective equipment must be selected according to the concentration
and amount of the dangerous substance at the specific workplace.
Respiratory protection:
Where risk assessment shows air-purifying respirators are appropriate use a fullface respirator with multi-purpose combination(US) or type ABEK (EN 14387) respirator cartridges as a backup to engineering controls. If the respirator is the sole means ofprotection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate
government standards such as NIOSH (US) or CEN (EU).
Control of environmental exposure
Prevent further leakage or spillage if safe to do so. Do not let product enter drains.
Discharge into the environment must be avoided--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 9 > Physical/Chemical Properties---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Physical State
Appearance
Odor
Odor Threshold
pH
Melting Point/Range
Boiling Point/Range
Flash Point
Evaporation
Flammability (solid,gas)Flammability or explosive limits
Upper
Lower
Vapor Pressure
Vapor Density
Specific Gravity
Solubility
Partition coefficient; n-octanol/water
Autoignition Temperature
Decomposition Temperature
Viscosity

LiquidClear, Blue-Violet
Odorless
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A

Component: Gel-Dissolving Buffer
Physical State
Appearance
Odor
Odor Threshold
pH
Melting Point/Range
Boiling Point/Range
Flash Point
Evaporation
Flammability (solid,gas)Flammability or explosive limits
Upper
Lower
Vapor Pressure
Vapor Density
Specific Gravity
Solubility
Partition coefficient; n-octanol/water
Autoignition Temperature
Decomposition Temperature

LiquidClear, Orange
Odorless
N/A
approx 4.0 - 6.0
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
-6ver. 1.0

Viscosity

N/A*N/A: not available--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 10 > Stability and Reactivity---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Reactivity:
No data available
Chemical stability:
This component is chemically stable under standard ambient conditions (room temperature) .
Possibility of hazardous reactions:
Violent reactions possible with: The generally known reaction partners of water.
Conditions to avoid:no information available
Incompatible materials:
No data available
Hazardous decomposition products:
In the event of fire: see section 5
Additional Information:
Not available
To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.
Other dangerous properties can not be excluded.
This substance should be handled with particular care.
Handle in accordance with good industrial hygiene and safety practice.

Component: Gel-Dissolving Buffer
Reactivity:
No data available
Chemical stability:
Stable under recommended storage conditions.
Possibility of hazardous reactions:
No data available
Conditions to avoid:No data available
Incompatible materials:
Strong acids, Strong oxidizing agents, Cyanides
Hazardous decomposition products:
Hazardous decomposition products formed under fire conditions. - Carbon oxides, Nitrogen oxides (NOx), Sulphur oxides,
Hydrogen cyanide (hydrocyanic acid), Ammonia
Other decomposition products - No data available
Hazardous decomposition products formed under fire conditions. - Carbon oxides, Nitrogen oxides (NOx), Sulphur oxides
In the event of fire: see section 5--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 11 > Toxicological Information---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Information on toxicological effects
MixtureAcute toxicity:
No data available
Symptoms: Possible symptoms:, mucosal irritations
Skin corrosion/irritation:
No data available
Serious eye damage/eye irritation:
Mixture causes serious eye irritation.
Respiratory or skin sensitization:-7ver. 1.0

No data available
Germ cell mutagenicity:
Carcinogenicity
Evidence of a carcinogenic effect.
IARC: No ingredient of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed
human carcinogen by IARC.
Reproductive toxicity
Specific target organ toxicity - single exposure:
No data available
Acute inhalation toxicity - Possible symptoms:, mucosal irritations
Specific target organ toxicity - repeated exposure:
No data available
Aspiration hazard:
No data available

Component: Gel-Dissolving Buffer
Information on toxicological effects
Acute toxicity:
No data available
Skin corrosion/irritation:
No data available
Serious eye damage/eye irritation:
No data available
Respiratory or skin sensitization:
No data available
Germ cell mutagenicity:
No data available
Carcinogenicity:
IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible orconfirmed human carcinogen by IARC.
Reproductive toxicity:
No data available
Specific target organ toxicity - single exposure:
No data available
Specific target organ toxicity - repeated exposure:
No data available
Aspiration hazard:
No data available
Additional Information:
RTECS: Not available
To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 12 > Ecological Information---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
Toxicity
Mixturedata available
Persistence and degradability:
No data available
Bioaccumulative potential:
No data available
Mobility in soil:
No data available
Results of PBT and vPvB assessment:This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very
persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.
Other adverse effects:-8ver. 1.0

No data available

Component: Gel-Dissolving Buffer
Toxicity:
No data available
Persistence and degradability:
No data available
Bioaccumulative potential:
No data available
Mobility in soil:
No data available
Results of PBT and vPvB assessment:This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very
persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.
Other adverse effects:
No data available
Harmful to aquatic life with long lasting effects.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 13 > Disposal Considerations--------------------------------------------------------------------------------------------------------------------------------------------------------------------Waste treatment methods
Product:
Offer surplus and non-recyclable solutions to a licensed disposal company. Waste material must be disposed of in accordance with
the Directive on waste 2008/98/EC as well as other national and local regulations. Leave chemicals in original containers. No
mixing with other waste. Handle uncleaned containers like the product itself.
Contaminated packaging:
Dispose of as unused product.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 14 > Transport Information---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Component: Crystal Violet Solution
UN number:
ADR/RID: 3082
IMDG: 3082
IATA: 3082
UN proper shipping name:
ADR/RID: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, LIQUID, N.O.S. (Crystal violet)
IMDG: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, LIQUID, N.O.S. (Crystal violet)
IATA: Environmentally hazardous substance, liquid, n.o.s. (Crystal violet)
Transport hazard class(es):ADR/RID: 9
IMDG: 9
IATA: 9Packaging group
ADR/RID: III
IMDG: III
IATA: III
Environmental hazards:
ADR/RID: yes
IMDG Marine pollutant: yes
IATA: yes
Special precautions for user
Further information
EHS-Mark required (ADR 2.2.9.1.10, IMDG code 2.10.3) for single packagings and combination packagings containing inner
packagings with Dangerous Goods > 5L for liquids or > 5kg for solids.Packages smaller than or equal to 5 kg / L , not dangerous
goods of Class 9

Component: Gel-Dissolving Buffer
UN number:
ADR/RID: 1760
IMDG: 1760
UN proper shipping name:
ADR/RID: CORROSIVE LIQUID, N.O.S. (Guanidinium thiocyanate)
IMDG: CORROSIVE LIQUID, N.O.S. (Guanidinium thiocyanate)
IATA: Corrosive liquid, n.o.s. (Guanidinium thiocyanate)Transport hazard class(es)ADR/RID: 8
IMDG: 8

IATA: 1760

IATA: 8-9ver. 1.0

Packaging group
ADR/RID: III
IMDG: III
IATA: III
Environmental hazards
ADR/RID: no
IMDG Marine pollutant: no
IATA: noSpecial precautions for user:
No data available--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 15 > Regulatory Information--------------------------------------------------------------------------------------------------------------------------------------------------------------------Component: Crystal Violet
Safety, health and environmental regulations/legislation specific for the substance or mixture
National regulatory information
Law concerning Pollutant Release
and Transfer Register / PRTR Law:
Industrial Safety and Health Law:
Chemical Substances Control Law:
Fire Service Law:
Poisonous and Deleterious Substances Control Law:

Not applicableNot applicableNot applicable for Specified Chemical Substance, Monitoring Chemical
Substance and Priority Assessment Chemical Substance.
Not applicableNot applicableComponent: Gel-Dissolving Buffer
Safety, health and environmental regulations/legislation specific for the substance or mixture
National regulatory information
Law concerning Pollutant Release
and Transfer Register / PRTR Law:
Industrial Safety and Health Law:
Chemical Substances Control Law:

Not applicableNot applicableNot applicable for Specified Chemical Substance, Monitoring Chemical
Substance and Priority Assessment Chemical Substance.
Fire Service Law:
Not applicable
Poisonous and Deleterious Substances Control Law:
Not applicable--------------------------------------------------------------------------------------------------------------------------------------------------------------------< 16 > Other Information--------------------------------------------------------------------------------------------------------------------------------------------------------------------DISCLAIMER
For R&D use only. Not for drug, household or other uses.
WARRANTY
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate
safety precautions. It does not represent any guarantee of the properties of the product. Biodynamics Laboratory Inc., shall not be held
liable for any damage resulting from handling or from contact with the above product.

- 10 ver. 1.0