Asian Workshop on Hydrodynamic and Thermodynamic Analysis of
Biological Macromolecules with SEDFIT and SEDPHAT
Workshop at the Yokohama City University, Yokohama, September 8-12+13, 2011, co-organized by

BMIA forum
Lecturers: Chad Brautigam (UT Southwestern, Dallas), Rodolfo Ghirlando (NIH, Bethesda), Grzegorz
Piszczek (NIH, Bethesda), Peter Schuck (NIH, Bethesda)
Organizing Committee: Fumio Arisaka (TIT), Susumu Uchiyama (Osaka University), Satoru Unzai (Yokohama
City University), Damien Hall (Tsukuba University)

Approximate Schedule
Saturday, September 8th
10:30 – 11:00 Registration11:00 – 11:30 Welcome/Announcements
11:30 - 12:30 Introduction to Analytical Ultracentrifugation: Experimental
12:30 – 13:30 Lunch Break
13:30 - 15:00 Lab Session 1. Running a Sedimentation Velocity Experiment
15:00 -15:20

Coffee Break

15:20 -15:50

Lab Session 2. Collection of Sedimentation Equilibrium Data

15:50 – 16:30 Introduction to Isothermal Titration Calorimetry
16:30 – 17:10 Introduction to Dynamic Light Scattering
17:10 – 18:00 Introduction to Fluorescence Spectroscopy
Sunday, September 9th
9:00 – 12:00

Theory and Practice of Sedimentation Velocity Analysis (1): Non-Interacting Species

10:30 – 10:50 Coffee Break
12:00 – 13:00 Introduction to SEDPHAT
13:00 -14:00

Lunch Break

14:00 – 15:00 Optimization and Statistical Error Analysis

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15:00 – 15:30 Basic ITC Data Processing and Analysis
15:30 – 16:00 Trivia Challenge (Round 1)
16:00 – 16:20 Coffee Break
16:20 – 18:30 Introduction of Participants – POSTER SESSION
Monday, September 10th
9:00 – 13:00

Theory and Practice of Sedimentation Velocity Analysis (2): Interacting Systems

10:30 – 10:50 Coffee Break
13:00 -14:00

Lunch Break

14:00 – 15:00 Theory and Practice of Sedimentation Velocity Analysis (3): Multi-signal analysis
15:00 – 17:30 Parallel Focus Sessions (Pre-Selection of up to 3 Topics Required, runtime ~40 min each, inparallel sessions, dependent on interest)§ Trace determination§ Advanced c(s) analysis§ Multi-Signal SV§ Isotherms Analysis§ Hands-On Lab Session I -III
§ Isothermal Titration Calorimetry (ITC)§ Surface Plasmon Resonance (SPR)§ Nanoparticles Analysis§ Basic c(s)§Fluorescence Spectroscopy§Membrane proteins

15:00 – 15:40 Focus Sessions I
15:40 – 16:00 Focus Sessions IIa
16:00 – 16:20 Coffee Break
16:20 – 16:40 Focus Sessions IIb
16:40 – 17:20 Focus Sessions III
Tuesday, September 11th
9:00 – 10:00

Theory of Sedimentation Equilibrium Analysis
Overview of SEDPHAT Structures and Strategies for SE Constraints

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9:45 – 10:30

Practical: Analysis of SE data in SEDPHAT

10:30 – 10:50 Coffee Break
10:50 – 11:30 Practical: Sedimentation Equilibrium Analysis of an Interacting System
11:30 – 12:15 Practical Error Analysis
12:15 – 13:00 Trivia Challenge (Round 2)
13:00 – 14:00 Lunch Break
14:00 – 16:00 Optional Individual Data Analysis Sessions I
16:00 – 16:20 Coffee Break
16:20 – 17:30 Optional Individual Data Analysis Sessions II
17:40 – 19:30 Get-together Dinner (Suzukake-Hall Lounge)
Wednesday, September 12th
9:00 – 9:30

Hydrodynamic Parameters Estimates from Crystal Structures

9:30 – 10:30

SPR Analysis of Binding Site Distributions in EVILFIT

10:30 – 10:50 Coffee Break (and group picture)
10:50 – 11:20 Practical: DLS Analysis in SEDFIT and SEDPHAT
11:20 – 12:00 Practical: Global ITC analysis of linked binding
12:00 – 12:15 Practical: Spectroscopy Data
12:15 – 13:00 Practical: Global Fitting of Data from Different Techniques
13:00 – 14:00 Lunch Break
14:00 – 14:30 Plotting utilities in SEDFIT and SEDPHAT
14:30 – 15:00 New and Special Features of SEDFIT and SEDPHAT
15:00 – 15:30 How to Simulate Data
15:30 – 16:00 Trivia Challenge (Final Round)
16:00 Final Discussion and Departure