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SARS-CoV-2 ANTIBODY DIRECTORY
THE COMPLETE GUIDE TO CHOOSING ANTIBODIES FOR
COVID-19 RESEARCH●●●●●●●●● ●●●●●●● ●●
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THE COMPLETE GUIDE TO SELECTING THE PROPER ANTIBODIESFOR YOUR COVID-19 STUDY●●●●●●●●●● ●●●●●●● ●●●● ●●●The ongoing COVID-19 pandemics has sickened 3.6

To support these studies, Sino Biological has developed

million people around the world, claiming overa large collection of antibodies against different

250,000 lives. To address this global public healthantigens of SARS-CoV-2. These antibodies can becrisis, unprecedented efforts are being made tostudy the culprit virus, SARS-CoV-2. The biomedicalcommunity are working to develop rapid diagnostic
reagents, vaccines, and therapies against this virus.

used to detect and/or neutralize the virus in a variety ofassays including ELISA, western blot, Immunoﬂuorescent
staining, Immunohistochemistry, SPR (Biacore andOctet), pseudovirus infection, and potentially in vivo
animal studies.

This guide book covers all available SARS-CoV-2 antibodies offered by Sino Biological. The readers can ﬁndtechnical information about their speciﬁcity and sensitivity, cross-reactivity, neutralizing potential, and theirvalidated applications. The overarching goal for this guide is to help the readers to choose appropriate
antibodies for their own experiments.

SARS-CoV-2 Antibody Buyer's Guide01

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SARS-CoV-2 ANTIGENS●●●●●●●●●● ●●●●●●● ●
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Same as all other coronaviruses, the genome of SARS-CoV-2 (2019-nCoV) encodes the spike protein, theenvelope protein, the non-structure proteins including the proteases and methyltransferases, the membraneprotein, and the nucleocapsid protein.

N-Protein

The spike protein (S-protein) is the common target for
Spikeprotein●●●●●●●●● ●●●●●● ●
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vaccines.

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contains two subunits, S1 and S2. S1 contains a receptor

contains other basic elements needed for the membrane●●●●●●●●● ●●●●●●●●●●● ●●●and binding with the cell surface receptor ACE2. S2 subunit●binding domain (RBD), which is responsible for recognizing●and●antibodies●●●●●●●●● ●●●●●●● ●
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neutralizing

S-Protein
RBDS2S1ACE2

SARS-CoV-2fusion.

The Nucleocapsid Protein (N-protein) is a highly

The methyltransferase is a non-structural protein.

isMethyltransferase mediates the methylation of thenormally very conserved. The SARS-CoV-2 Nviral RNA, which may help the virus evade the hostprotein is often used as a marker in diagnostic

immune system. Methyltransferase inhibitor mayassays.

work as an anti-viral treatment.

The papain-like protease (PlPro) is a protease. It

Angiotensin-converting enzyme 2 (ACE2) binds

non-structural proteins like replicase polyprotein.

SARS-CoV and HCoV-NL63. This interaction is

PlPro, and 3CLPro. PlPro is a target for antiviral drug

mediated by the RBD domain of the spike

development.

protein, and is believed to be the pivotal event in

immunogenic

phosphoprotein,

and

it

mediates the post-translational processing of otherwiththeSpikeproteinofcoronaviruses

the membrane fusion process.

02SARS-CoV-2 Antibody Buyer's GuideSino Biological Inc.

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ELSIA Antibody Pairs

Pairs Ⅰ
Cat #40143-R004 (Capture)

Pairs Ⅱ
Cat #40143-MM05 (Capture)

Description Anti-Nucleocapsid

Description Anti-Nucleocapsid

Type

Rabbit MAb

Type

Mouse MAb

Ig TypeRabbit IgG

Ig Type

Mouse IgG1

Cat #40143-R040 (Detection)

Cat #40143-R001 (Detection)

Description Anti-Nucleocapsid

Description Anti-Nucleocapsid

Type

Rabbit MAb

Type

Rabbit MAb

Ig TypeRabbit IgG

Ig TypeRabbit IgG

W

Optical Density (450nm)

E

NUCLEOCAPSID (N) PROTEIN ANTIBODIES

10.1

1001000

2019-nCoV-N Concentration (pg/mL)

Western Blot Antibodies

Cat #40143-R019

Description Anti-Nucleocapsid
Type

Rabbit MAb

Ig TypeRabbit IgG

KDa
95
72
55
43gng ng ng ng 1ng .5n30 10 5 20Sample:
Recombinant SARS-CoV-2
Nucleocapsid

3426

FULL LIST OF ANTI-NUCLEOCAPSID ANTIBODIESCat #Description

TypeIg Type40143-R001

Anti-Nucleocapsid

Rabbit MAb

Rabbit IgG

40143-R040

Anti-Nucleocapsid

Rabbit MAb

Rabbit IgG

40143-R004

Anti-Nucleocapsid

Rabbit MAb

Rabbit IgG

40588-R0004

Anti-Nucleocapsid

Rabbit MAb

Rabbit IgG

40143-R019

Anti-Nucleocapsid

Rabbit MAb

Rabbit IgG

40143-MM05

Anti-Nucleocapsid

Mouse MAb

Mouse IgG1

40143-MM08

Anti-Nucleocapsid

Mouse MAb

Mouse IgG1

40588-T62

Anti-Nucleocapsid

Rabbit PAb

Rabbit IgG

SARS-CoV-2 Antibody Buyer's Guide03

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Antibodies against the S1 and RBD Domains
E

ELSIA Antibody Pairs

Pairs ⅠN

Neutralization Antibodies
40591-MM43

40150-D001 (Capture)

Description

Anti-Spike S1Type

Mouse MAb

Type

Chimeric MAb

Ig Type

Mouse IgG1Ig Typemouse v domain / human IgG1

Cat #40592-MM57

Cat #40150-D003 (Detection)

Description

Anti-Spike RBD

TypeMouse MAb

Ig Type

Mouse IgG2b

Cat #Description

Description

Anti-Spike RBD

Anti-Spike RBD
Chimeric MAb

Ig Typemouse v domain / human IgG1

Optical Density (450nm)

Type

10.1

Cat #
Description

1001000

10000

60
40200.01

0.1110100

Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody was
detected by SARS-CoV-2 Inhibitor Screening ELISA Kit (Catalog#KIT001). The IC50 is typically 0.857 nM.

I40150-D002 (Capture)
Anti-Spike RBDType

Chimeric MAb

Ig Typemouse v domain / human IgG1Cat #40150-D004 (Detection)

Description

80Conc.(nM)2019-nCoV-S1 Concentration (pg/mL)

Pairs Ⅱ

10001E-30.01

Anti-Spike RBD

Type

Chimeric MAb

Ig Typemouse v domain / human IgG104

Percent Activity(%)

Cat #IF Antibodies

Cat #40150-R007

Description

Anti-Spike S1

Type

Rabbit MAb

Ig TypeRabbit IgG

IF staining of SARS-CoV-2
Spike in ACE2-overexpressed
293T cells, infected bySARS-CoV-2-Spikepseudovirus (PSV).

SARS-CoV-2 Antibody Buyer's GuideSino Biological Inc.

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High Afﬁnity Antibodies

Antibodies against the S2 Protein

Cat #40150-D005

Cat #40590-D001

Description

Anti-Spike RBD

Description

Anti-Spike S2

Type

Chimeric MAb

Type

Chimeric MAb

Ig Typemouse v domain / human IgG1

Ig Typemouse v domain / human IgG1

1.60
1.40nm1.20
1.00This is a chimeric antibody speciﬁcally recognizing the S2domain of SARS-CoV-2. The speciﬁcity assay is assessed in
ELISA which shows no cross-reactivity with SARS-CoV-2 S1,RBD or MERS-CoV S2.

0.80
0.60
0.40
0.20
0.00

050

100 150 200 250 300 350 400 450 500 550 600

Time(sec)

The binding between immobilized SARS-CoV-2 RBD protein and theanti-Spike antibody 40150-D005 in Octet RED assay

FULL LIST OF ANTI-SPIKE ANTIBODIESCat #Description

TypeIg Type40591-MM43

Anti-Spike S1

Mouse Mab,Neutralizing Antibody

Mouse IgG1

40150-R007

Anti-Spike S1

Rabbit MAb

Rabbit IgG

40150-D001

Anti-Spike S1Chimeric MAbmouse v domain / human IgG1

40150-D002

Anti-Spike S1Chimeric MAbmouse v domain / human IgG1

40150-D003

Anti-Spike S1Chimeric MAbmouse v domain / human IgG1

40150-D004

Anti-Spike S1Chimeric MAbmouse v domain / human IgG1

40150-D005

Anti-Spike S1Chimeric MAbmouse v domain / human IgG1

40150-D006

Anti-Spike S1Chimeric MAbmouse v domain / human IgG1

40592-MM57

Anti-Spike RBD

Mouse Mab,Neutralizing Antibody

Mouse IgG2b40592-T62

Anti-Spike RBD

Rabbit PAb

Rabbit IgG

40590-D001

Anti-Spike S2Chimeric MAbmouse v domain / human IgG140590-T62

Anti-Spike S2

Rabbit PAb

Rabbit IgG

40589-T62

Anti-Spike

Rabbit PAb

Rabbit IgG

SARS-CoV-2 Antibody Buyer's Guide05

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SARS-CoV-2 shares 80% sequence identity to the 2003 SARS-CoV. Hence, antibodies raised against SARS-CoV-2antigens often shows various degree of cross-reactivity with the corresponding antigens of 2003 SARS-CoV. On theother hand, the similarities between SARS-CoV-2 and other members of human coronavirus are very limited. Thefollowing two clones of anti-spike antibodies have been tested against all seven coronavirus, and are shown to bespeciﬁc to SARS-CoV-2 spike.

The reactivity of the antibodies against the known coronavirus is assessed in ELISA. The reactivity (OD450)
is illustrated in the ﬁgure below.

3.000

3.000-OC

43E
HCoV-2oVHC

HCoV-N

L629

31KU

SoV-H

ER
M

-2oV
SA

RS-C

C

HC

oV-O-2

43

E29

3oV

HC

HC

oV-N

L6KU

S-H

ER

HC

oV

M

SAoV-C
RS

10.000RS

0.000-20.500

SA06

1.000

0.500

HC

1.000

1.500

RS

1.500

SA

OD450

2.500
2.000

OD450

2.500
2.000

Cat #40592-MM57

Cat #40591-MM43

DescriptionSpike RBD Antibody

Description

Spike S1 Antibody

TypeMouse MAb

TypeMouse MAbIg Type

Monoclonal Mouse IgG2bIg Type

Monoclonal Mouse IgG1

Application

ELISA, Neutralization

Application

ELISA, Neutralization

SARS-CoV-2 Antibody Buyer's GuideSino Biological Inc.
Address: Building 9, No.18 Kechuang 10th St, BDA
Beijing, 100176, P.R.China
Tel: +86-400-890-9989
Fax: +86-10-5095-3282
Website: www.sinobiological.com
Email: order@sinobiological.com