Workflow Guide

freeslate jr.

Biologics formulation
Walk up, set up your run and walk away. Freeslate jr. automates the manual measurements you do one by one at thebench. Choose a combo of high-throughput pH with visualinspection, visual inspection with viscosity or viscosity withpH. Crank through more samples in a single day, get themdone the same way every time and check out a broader
developability and formulation space.

Applications• Rapidly characterize a wide range of protein formulations
with limited material
freeslate jr. configured for biologics formulation• Perform developability and preformulation screens• Evaluate formulation robustness• Formulation development• Manage and track formulations and analytical results tofacilitate rapid scientific decisions

Key features• Automate pH, viscosity and visual inspection• Use a wide range of vials and 96-well plates• Check the pH of a 96-well plate in approximately
30 minutes

Visual inspection
stationMulti-channel pHprobes

Viscosity station• Measure viscosity of protein formulations up to
1,000 cP with only 100 µL of sample
1• Grab color, turbidity and visible particle count all in
one shot• Capture images and archive them for easy re-analysis

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7Example freeslate jr. deck configured for biologics formulation
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Vial/plate gripper
Waste bin
Viscosity station
Tool and plate rack

5 3-Position plate rack
6 Visual inspection station
7 Vial/plate hotel

Workflow Guide

Available options
Visual inspection station (VIS) analyses

Vial/plate gripper

Includes:• Visual particle analysis• Turbidity• Color measurement

Plate size: Standard microtiter
Vial size: 1–125 mL
Total mass: Up to 3 kgVial size: 2–20 mL
Recommended sample volume: 1 mL in 2 mL serum vial
Measurement time: 2–3 min per vial

Technology: Positive displacement tip (PDT)
Disposable tip capabilities: 100 µL from Rainin

Suspended visible particle detection
Minimum particle size detected: 80 μm
Maximum solution viscosity: 30–35 cP
Particle count accuracy:• No particles: 0 particles detected• 1–3 particles: Detect at least 1 particle• 4–9 particles: Actual particle count ±2 particles• 10–25 particles: Actual particle count ±5 particles

Turbidity
Measurement range: 10–1000 NTU
Measurement accuracy: ±5 NTU (for non-absorbing samples)
Repeatability: ≤3 NTU for 10 consecutive samples

Color measurement
Color: Correct match of Euro Pharmacopeia BY1–BY7 standards

pH measurement
Configuration: Single or 4-channel probe
Measurement time per 96-well plate: ~ 34 minutes
Range: pH 1–13
Resolution: 0.01 pH units
Repeatability: ±0.1 pH units

Viscosity station
Measurement range: 2–100 cP
Accuracy: ±0.5 cP + 10% of the actual viscosity
Repeatability: StDev <0.5 cP + 5% of mean
Sample volume: 100 μL
Minimum volume in well: 200 μL
Temperature range: 4–40 °C
Temperature accuracy: ±1 °C
Measurement time: 6 min/sample
Throughput: 10 samples/hUnchained Labs
6940 Koll Center Pkwy, Suite 200
Pleasanton, CA 94566
Phone: 1.925.587.9800
Toll-free: 1.800.815.6384
Email: info@unchainedlabs.com

Viscous liquid dispenser

Vortexing station
Orbital: Up to 2000 rpm max
Maximum vortexing mass: 860 g

Off-deck third-party instrument virtual integration• DLS• HPLC• cIEF
Other systems available for virtual integration.
Please contact Unchained Labs for a full list of systems.

Facilities requirements
Physical• With integrated enclosure:105 cm W x 90.4 cm D x 140 cm H ~150 kg• With integrated table option:
167 cm W x 90.4 cm D x 200 cm H ~240 kg
Electrical• freeslate jr.
110–220 ±10% VAC, 50–60 Hz, 16 A
• Computer:
US: 115 V ±10 %, 60 Hz, 10 A
EU: 220–230 V ±10 %, 50 Hz, 16 A
Compressed dry air: 0.5–0.9 MPa (70–130 PSI), 16L/min(8 mm hose)© 2016 Unchained Labs. All rights reserved. Freeslate jr. is a trademark
and Unchained Labs is a registered trademark of Unchained Labs. Allother brands or product names mentioned are trademarks owned bytheir respective organizations.
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