★★★ ＜第19回知的財産翻訳検定試験【第9回英文和訳】＞ ★★★

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問１．請求項***START***から***END***までを和訳してください。

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1.

A method of combining mesenchymal stem cells with an osteochondral

allograft, the method comprising:obtaining adipose tissue having the mesenchymal stem cells together
with unwanted cells;
digesting the adipose tissue to form a cell suspension having themesenchymal stem cells and the unwanted cells;
adding the cell suspension with the mesenchymal stem cells to theosteochondral allograft so as to form a seeded osteochondral allograft; andallowing the cell suspension to adhere to the osteochondral
allograft for a period of time to allow the mesenchymal stem cells to attach.

2.

A method in accordance with claim 1, wherein the osteochondral

allograft is from a cadaveric donor, and the step of obtaining the adipose

tissue includes recovery from the same cadaveric donor as theosteochondral allograft.

3.

A method in accordance with claim 1, wherein the step of digesting theadipose further includes aspirating a supernatant containing mature adipocytes
so as to provide a pellet.

***END***問２．背景技術***START***から***END***までを和訳してください。

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Sleep has been implicated in the plastic cerebral changes that underlie
learning and memory. Both rapid eye movement (REM) and non-REM sleep (NREM)
play important roles in memory. Behavioral observations in rats showthat periods of learning are associated with subsequent increases in REM sleep,
whereas REM sleep deprivation impairs memory of cognitive procedural or implicit
types of material previously learned. NREM was found to be positivelycorrelated with the ability to retain a word pair-association list which wasa declarative memory. In addition, the transition from short-term to long-term
memories by reactivation of sharp wave-ripples in the hippocampus during NREM
was important for memory consolidation. It has also been demonstrated thatinducing slow oscillation-like potential fields by transcranial applicationof oscillating potentials (0.75 Hz) during early nocturnal NREM, enhances theretention of hippocampus-dependent declarative memories in healthy humans.

***END***問３．実施の形態***START***から***END***までを和訳してください。

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The term "primer" refers to an oligonucleotide, whether occurring
naturally as in a purified restriction digest or produced synthetically, thatis capable of acting as a point of initiation of synthesis when placed underconditions in which synthesis of a primer extension product that iscomplementary to a nucleic acid strand is induced, (e.g., in the presence ofnucleotides and an inducing agent such as a DNA polymerase and at a suitabletemperature and pH). The primer is preferably single stranded for maximum
efficiency in amplification, but may alternatively be double stranded.
If double stranded, the primer is first treated to separate its strands before
being used to prepare extension products. Preferably, the primer is anoligodeoxyribonucleotide. The primer must be sufficiently long to prime thesynthesis of extension products in the presence of the inducing agent.
The exact lengths of the primers will depend on many factors, includingtemperature, source of primer, and the use of the method.
As used herein a "nucleotide locus" refers to the location of a
nucleotide in a nucleic acid molecule. A nucleotide locus of a methylated
nucleotide refers to the location of a methylated nucleotide in a nucleic
acid molecule.

***END***問４．実施例***START***から***END***までを和訳してください。

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Replicated groups of 15 termite workers were weighted for
initial body weight, before they were placed in a 35cm Petri dish. To reducestress, the bottom surface of each Petri dish was scratched using a utility
knife to provide footing for termites. Testing materials were dried at 80 deg.Covernight and weighted. To examine the susceptibility of these testingmaterials to termites, they were used as the only food source (a "no choice"test). Materials were soaked in distilled water for 1 hour before being
introduced into the Petri dish. The bioassay was performed in complete darkness
for 10 days. A total of four technical replications were performed for eachtreatment in each colony (60 termites/treatment/colony).
Mortality and body weight of termite workers were monitored throughout
the experiment. Cumulative mortality at the 5th and 10th day, as well as thebody weight of survived workers at the 10th day was documented. Mortality data
at day-5 is reported for the experiments.

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