container handling method
Collecting and handling methods of samples
Blood[Timing of blood sampling] Ideally, blood samples are collected when well-rested and after fasting in the earlymorning (except for emergency situations or specified conditions).
[Collecting samples]◯ Blood: After taking blood, promptly transfer to a specified tube containing the anticoagulant, and gently
invert the tube. Unless otherwise specified, specimens to be stored at room temperature or underrefrigeration should be kept in the original tubes, and those to be stored frozen should be transferredto submission containers. Submit the specimens under the specified storage conditions.
◯ Serum: Collect about 3 times the volume of blood needed for the test.
Unless otherwise specified, allow to stand at room temperature. After coagulation, centrifuge the blood.
After centrifugation, transfer the supernatant to a submission container. Submit the specimens under
the specified storage conditions.
The storage condition depends on the tests, so please check it for each test.
◯ Plasma: Collect about 3 times the volume of blood needed for the test.
Unless otherwise specified, after drawing blood into a specified tube containing the anticoagulant,
promptly invert the tube, and centrifuge the blood.
After centrifugation, transfer the supernatant to a submission container. Submit the specimens under
the specified storage conditions.
Sampling methods and storage conditions depend on the tests, so please check comments,
storage conditions, and handling methods of containers for each test.
If the volume of the collected blood is less than the volume defined in the container, it may give
influence on the data of some items. Make sure to collect the specified volume of blood.
[Points to note]◯ Blood sampling into vacuum tubes
If collected blood volume is below the specified volume of the tube, the pressure inside the tube remains
negative, which may cause hemolysis. Make sure to collect the specified volume of blood.
◯ Blood sampling into syringes
Take off the needle from the syringe, and slowly inject the needle. Draw blood along the side of the tube.
◯ Preventive measures against hemolysis
Avoid putting excess pressure or foaming of blood when drawing blood. Use a completely dried blood
sampling tube.
Avoid physical stimulation (e.g., extremely high or low temperature, vibration, etc.).

Urine

container handling method[Random urine]
Collect urine into a urine sampling container, and transfer the necessary volume of urine into a submission
container. Submit the specimen under the specified storage conditions.
Some tests specify the timing of urine sampling; therefore, please check the comments and handling methods ofdedicated containers for each test.
[24 hours urine collection]
At the starting time point of urine collection, instruct a patient to urinate completely (that should be flushed).
Then, collect all urine in a storage container in the following 24 hours period. During the time of urine collection,
the storage container should be covered by a lid and kept in a cold and dark place.
After the urine collection, measure the urine volume, mix well, and transfer the necessary volume of thespecimen into a submission container. Write the collection time period and urine volume, and submit the
specimen under the specified storage conditions.
○ Acidified urine collection
Depending on the tests, SRL uses following methods of acidified urine collection to preserve andstabilize specimens. For some tests, acidified urine may have an effect on the data; therefore, please
make sure to check comments for each test.
1. Using preservatives
Regardless of the urine volume, put all dedicated preservatives (the tablet and granules) into a storage
container for urine collection.
2. Using hydrochloric acidPut the specified amount of 6N hydrochloric acid into a storage container for urine collection. (Pleasecheck comments for the specified amount of hydrochloric acid and points to note for each test.)
Same time on the next day
Starting time
End
Start
24 hours urine collection (Collect urine also when having a bowel movement.)
Urinate(Discard)①

Collect urine(Even if a patient does not feel like urinating)container handling method
Sending in samples for coagulation test (extraction from consensus on handling of coagulation test samples)[Blood collection tube]
・Use the tube made of plastic or glass treated with silicon.
・Use 0.105-0.109M (3.13-3.2%) sodium citrate solution for anticoagulant.
・The ratio of sodium citrate solution to blood is 1: 9. Acceptable volume of blood collection should be nominal
capacity±10%.
・For patients with hematocrit level (Ht)≥55%, adjust the volume of sodium citrate solution.
[Blood collection]
Comply with JCCLS standard blood collection guideline GP4-A3.
・Vacuum or syringe blood collection can be done.
○ Vacuum blood collection using a blood collection needle: collect first blood specimen using a coagulation
test tube or blood serum collection tube.
○ Vacuum blood collection using a butterfly needle: collect first blood specimen using a dummy bloodcollection tube or a blood collection tube for other tests before blood collection in a tube for coagulation
test.
○ Syringe blood collection: Transfer first blood specimen into a blood collection tube for coagulation test.
・Aseptic needling should be applied with minimum blood flow stagnation (use of tourniquet).
・Use 21- 23G injection needle or butterfly needle depending on individual situations.
・Don’t use a venous line that contains heparin.
・Confirm that the accurate volume of blood has flown into the blood collection tube. Invert the tube immediately
5 times to mix the blood and anticoagulant without forming bubbles.
[centrifugation]
・The temperature setting for centrifugation should be controlled to room temperature (18-25℃ ).
・Average centrifugal gravity of 1500 x g for at least 15 minutes or 2000 x g for at least 10 minutes isrecommended for the centrifugation treatment.
・ Contamination with platelet affects the measurement result. Use supernatant at least 5mm away from thebuffy coat so that the remaining platelet count is less than 1000/μL.
[Confirmation of coagulated samples]
Samples may be coagulated at the time at the time of arrival in the laboratory, especially when taken frompatients with difficulty of blood collection. If coagulation is confirmed before centrifuge, whether the test will
be continued after re-collection of blood or discontinued will be discussed with the clinician.
[Supplementary information]
According to the CLSI Approved guideline 5th ed H21-A5, blood collection via a vascular access device (VAD)
should be done with no air leak confirmed, avoiding heparin flush wherever possible to prevent contamination or
dilution with heparin.
More specifically, flush with 5mL of saline, and discard blood of 5 mL or 6-folds of VAD dead space volume
before sample collection.
It is clearly stated that blood collection from saline lock (cap-off intravenous port) should be done after
discarding twice the volume of dead space of catheter and the extension set.
〈Reference〉
1. Coagulation test standardization working group, Standardization Committee of Japanese Society forLaboratory Hematology Consensus on handling of coagulation test samples, Journal of The Japanese Society
for Laboratory Hematology 17, 149-168, 2016.
2. Ieko M et al：expert consensus regarding standardization of sample preparation for clotting time assays. Int
J Hematol 112; 614-620, 2020.

The dedicated containers presented in each page are prepared by SRL. Please contact the nearest office for the containers.
Please regard the self-life as a reference.

Split Sales
Based on the customer's instruction, we may open the package and sell the product in small packages (installment
sales). In such cases, the customer shall agree to the following.
(ⅰ) The manufacturer may not guarantee the quality of containers subject to installment sales within the
expiration date disclosed by the manufacturer or stated in the statement of performance.
(ⅱ) Containers subject to installment sales must be used promptly after receipt.
(ⅲ) We are not legally liable for non-conformity of contract with respect to the containers sold in installments.

Handling of labeled containers
We may affix a predetermined label to a container upon instruction from the customer. In such cases, ownershipof the container to which the label is attached shall be transferred to the customer at the time the customer
places an order for the container, notwithstanding anything stated in the contract or purchase order for thesale of the container.

②

container handling method

About dedicated containerscontainer handling method
CONTAINER FORM

A00

Storage conditions: Room temperature

Previous container symbol

X

Polyethylene test tube

CONTAINER FORM

ARR

Storage conditions: Room temperature

Previous container symbolr

Sterile polyethylene test tube

CONTAINER FORM

ASS

Storage conditions: Room temperature

Previous container symbolicontainer handling method

Light-shielding polyethylene test tube

CONTAINER FORM

AZZPrevious container symbol

Acid-washed polyethylene test tube
Storage conditions: Room temperature

Z

Metal test③

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

F00
Previous container symbol

U

stool
500mg

Adenovirus DNA

F

Stool container Norovirus
Storageconditions:Roomtemperature

antigen

Norovirus RNA,
qualitative

Collect 500mg of stool in thedesignated container, and be sure to
store frozen.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

stool
Little-finger headsize

Place 0.5g (little-finger head size) ofstool collected from center of stool
into a container shown in the left
image, and be sure to freeze.

stool
1g

Place 1g (thumb-head size) of stool
into a container shown in the left
image, and be sure to freeze.

Fecal
calprotectin

CONTAINER FORM

SAMPLE HANDLING METHOD

Digestion state

stool
Thumb-head size

R

Entamoeba
histolytica DNA
qualification

stool
0.5g

F

TEST NAMESTORETEMPERATURE

Place 1g (thumb-head size) of stool
into a container shown in the left
image, and refrigerate the specimen.
Place 0.5g (little-finger head size) ofstool collected from center of stool
into a container shown in the left
image, and be sure to freeze.

SAMPLE HANDLING METHOD

F20Previous container symbol

d4

Dedicated container for Fecal
Helicobacter pylori antigen

Fecal Helicobacter
pylori antigen(Note) Please submit as fresh stool as possible.
(Note) Do not remove or loosen the green cap of thestool collection container.

container handling method

Contents: Boric acid 1mL
Storage conditions: Room temperature
Shelf-life: 24 monthsR

1. ( Preparation) Peel off the white label from thecontainer halfway and write name, date, etc., thenroll it up again. Turn the pink stool collection stick
and pull it out.
2. (Collect stool) Scrape the surface of the stool sothat all the grooves on the tip of the stick are filledwith stool.
3. (After collecting stool) Put the stick back into thecontainer and screw it firmly to the base. Do notopen again.
4. (Storage Precautions) Store in the attached plastic
bag in a cool, dark place until submission. After
collecting stool, please submit it as soon as possible.

④

container handling method
CONTAINER FORMTEST NAMESTORETEMPERATURE

F30

Fecal calprotectin(FEIA)F

Previous container symbol

d9

SAMPLE HANDLING METHOD

Twist

Holding

1. W
 hile holding the blue part of the cap, 2. Scrape the stool to completely fill up
twist the top light blue part to the left
the 4 grooves at the tip of the stick.
to pull out the stick.

Remove thestool at the tip

clicking
sound

Contents:
tris buffer
BSA
sodium azide (≤ 0.1%)
Storage conditions:
Refrigeration
Shelf-life: 24 months

CONTAINER FORM

3. R
 emove the stool at the tip with toilet 4. P ut the stick into the container and twist
paper, etc.
the light blue part to the right until it fits
into the blue part with a clicking sound.
● NoteDo not expose eyes, mouth or skin to the preservation solution in the container. If
accidentally exposed, rinse well with water.

TEST NAMESTORETEMPERATURESAMPLING METHOD

F80
Previous container symbol

d8Hemoglobin andtransferrin in feces

container handling method

Hemoglobin infeces, qualitative
Gold colloid
methodHemoglobin in

Additives: Preservation solution feces, quantitative
Gold colloid
Storage conditions: Room temperature method
Shelf-life: 1 year⑤

● Sampling methodsR

1. Rotate the screw cap, and pull out the stick. Scrape
the wide surface of the stool with the samplingprobe.
2. Place the stick back into the container (do notreinsert), and firmly tighten the screw cap.
3. Put the container in an attached bag. Then,
refrigerate and submit the specimen.

● Points to note

1. Do not throw away preservation solution in thecontainer.
2. Cover the grooved portion of the sampling probe
completely with stool sample. Do not scrape up toomuch or too little stool.
No food restrictions are necessary before fecal
sampling.

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Leukemia/lymphoma
analysis (LLA), CD45
gating, test forhematopoietic
malignant tumor cells

H00
Previous container symbol

H

Multiple myeloma
analysis CD38
multianalysis, test forhematopoietic
malignant tumor cells
Chromosome
G-BandingContaining thepreservation
solution(Container
capability of
5 mL)
Additives:
RPMI-1640
FBS
Kanamycin
sulfateNovo-heparin
sodium
Sodiumbicarbonate
HEPES
Storageconditions:FreezeChromosome
analysis using
spectral karyotyping(SKY)(Hematologicaldisorder)SAMPLE HANDLING METHOD

Collect the specified amount ofsample, mix well, and refrigerate.
Please submit a sample on the day it
is collected.

bone marrow
bone
fluid
marrow (with thefluid preservation
1.0 each solution)
1.0 eachObtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

TCF3-PBX1 t(1;19)translocation
CKS1B 1q21
amplification
ALK 2p23translocation
GATA2-MECOM
inv(3) inversion,t(3;3) translocation

lymph node lymph node (with the
5×5×5 preservation solution)mm
5×5×5 mmbone
bone marrow fluid
marrow (with thefluid 1.0 preservationeach solution) 1.0
each

Shelf-life:
Use this after
lymph node lymph node (with theconfirming
5×5×5 preservation solution)that themm
5×5×5 mm
BCL6 3q27
solution color translocation
has turned topale pink.
The color
turns to pale IGH-FGFR3 t(4;14)
yellow after translocation
freezing and
FIP1L1-PDGFRA
to pale pinkafter
4q deletion
thawing.
(4q12 deletion)

EGR1 5q
deletion
PDGFRB 5q32
translocationbone marrow
bone
fluid
marrow (with thefluid preservation
1.0 each solution)
1.0 eachR

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.
Place lymph nodes 5×5×5 mm in size in a container
shown in the left image, suspend, and refrigerate.
Please submit a sample on the day it is collected.

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

D7S486 7q deletion/
Chromosome 7monosomy
Chromosome anomaly
associated with hematologicaldisorders, Chromosome 8⑥

container handling method

CSF1R 5q
deletion

Place lymph nodes 5×5×5 mm in size in a container
shown in the left image, suspend, and refrigerate.
Please submit a sample on the day it is collected.

container handling method
CONTAINER FORMTEST NAME

H00
Previous container symbol

H

MYC 8q24translocation

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

bone marrow
bone
fluid
marrow (with thefluid preservation
1.0
solution)
1.0

lymph node
5×5×5 mmeach

IGH-MYC t(8;14)translocation
Containing thepreservation
solution(Container
capability of
5 mL)

RUNX1-RUNX1T1(AML1-MTG8)t(8;21) translocation

Additives:
RPMI-1640
FBS
Kanamycin
sulfateNovo-heparin
sodiumSodiumbicarbonate
HEPES

BCR-ABL1t(9;22)translocation
KMT2A(MLL)
11q23.3translocation

Storageconditions:FreezeFGFR1 8p11.2
translocation

IGH-CCND1(IGH-BCL1)t(11;14)translocation

container handling method

Shelf-life:
Use this after
confirming
NUP98 11p15
that thesolution color translocation
has turned topale pink.
The color
turns to pale BIRC3-MALT1
yellow after (API2-MALT1)
freezing and t(11;18)
to pale pinktranslocation
after
thawing.

lymph node(with thepreservation
solution)
5×5×5 mm each

SAMPLE HANDLING METHOD
Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.
Place lymph nodes 5×5×5 mm in sizein a container shown in the left image,suspend, and refrigerate.
Please submit a sample on the day it
is collected.

bone marrow
bone
fluid
marrow (with thefluid preservation
1.0 each solution)
1.0 eachObtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

lymph node(with thelymph nodepreservation
5×5×5 mmsolution)
5×5×5 mmPlace lymph nodes 5×5×5 mm in sizein a container shown in the left image,R
suspend, and refrigerate.
Please submit a sample on the day it
is collected.

bone marrowbone
fluid (with themarrow
preservation
fluid
solution)
1.0 each
1.0 eachObtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

lymph node(with thelymph nodepreservation
5×5×5 mmsolution)
5×5×5 mmPlace lymph nodes 5×5×5 mm in sizein a container shown in the left image,suspend, and refrigerate.
Please submit a sample on the day it
is collected.

ATM 11q
deletion
Chromosome
bone marrowanomalybone
fluid
associated with marrow (with thefluid preservation
hematological
1.0 each solution)
disorders,
1.0 each
Chromosome 12
ETV6-RUNX1 (TELAML1) t(12;21)translocation
D13S319 13q
deletion⑦

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

container handling method
CONTAINER FORMTEST NAME

H00
Previous container symbol

H

IGH-BCL2t(14;18)translocation

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

lymph node(with thelymph nodepreservation
5×5×5 mmsolution)
5×5×5 mmbone marrow
bone
fluid
marrow (with thefluid preservation
1.0 each solution)
1.0 each

IGH-MAFt(14;16)translocation
Containing the PML-RARA
preservationt(15;17)bone marrowsolutiontranslocation
bone
fluid
(Container
marrow(with
the
CBFB inv(16)capability of
fluid preservation
inversion, t(16;16) 1.0
5 mL)
each solution)translocation
1.0 each

SAMPLE HANDLING METHOD
Place lymph nodes 5×5×5 mm in sizein a container shown in the left image,suspend, and refrigerate.
Please submit a sample on the day it
is collected.

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

Additives:
TP53 17p deletion
RPMI-1640
FBS
lymph node
Place lymph nodes 5×5×5 mm in size
Kanamycin(with thein a container shown in the left image,
lymph
node
sulfatepreservationsuspend, and refrigerate.
5×5×5 mmNovo-heparinsolution)
Please submit a sample on the day it
sodium
5×5×5 mmis collected.
MALT1
18q21
Sodiumbone marrow
Obtain 1.0 mL of bone marrow fluid
translocation
bicarbonate
bone
fluid
into a container shown in the left
HEPES
marrow (with theimage aseptically, mix well, andfluid preservation R refrigerate the specimen.
Storage
1.0 each solution)
Please submit a sample on the day it
conditions:
1.0 eachis collected.
Freezelymph node
Place lymph nodes 5×5×5 mm in size(with thein a container shown in the left image,
Shelf-life:
lymph nodepreservationsuspend, and refrigerate.
Use this after
5×5×5 mmsolution)
Please submit a sample on the day it
BCL2 18q21
confirming
5×5×5 mmis collected.
translocationthat thesolution color
has turned topale pink.
The color
20q deletion
turns to paleyellow after Chromosome
freezing and anomaly associatedto pale pink
with hematologicalafter
disorders,
thawing.

bone marrow

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

container handling method

bone
fluid
Chromosome X
marrow (with the
Chromosome
fluid preservation
anomaly associated 1.0 each solution)
1.0 eachwith hematologicaldisorders,
Chromosome Y
Sex-mismatchedbone marrowtransplantation(BMT)(Chromosomes XY)⑧

container handling method
CONTAINER FORM

H00
Previous container symbol

H

Containing thepreservation
solution(Container
capability of
5 mL)

TEST NAME
1p Deletion

Additives:
RPMI-1640
MYCN 2p24
FBS
amplification
Kanamycin
sulfateNovo-heparin
sodiumSodiumbicarbonate
HEPES
Storageconditions:FreezeSAMPLE HANDLING METHOD

tissuestissues(with the
5×5×5 preservationmm
solution)for each 5×5×5 mm
eachPlace tissues5×5×5 mm in size ina container shown in the left image,suspend, and refrigerate.
Please submit a sample on the day it
is collected.

bone marrow
bone
fluid
marrow (with thefluid preservation
1.0
solution)
1.0

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.
R

19q Deletion

Shelf-life:
Use this after
confirmingthat thesolution color
has turned topale pink.
The color
EWSR1
turns to pale 22q12
yellow after translocation
freezing andto pale pinkafter
thawing.

container handling method⑨

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

tissuestissues(with the
5×5×5 preservation
mm forsolution)each
5×5×5 mmeach

Place tissues5×5×5 mm in size ina container shown in the left image,suspend, and refrigerate.
Please submit a sample on the day it
is collected.

bone marrow
fluid
bone
marrow (with thefluid preservation
1.0 each solution)
1.0 eachObtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

container handling method
CONTAINER FORM

H00

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

FLT3/ITD
mutation analysis

Previous container symbol

H

FLT3 mutational
analysis ITD/TKD

Draw blood to obtain 1.0 mL ofbone marrow fluid into a container
shown in the left image, mix well,and refrigerate the specimen. Avoid
putting in another request at the sametime.
In this method, the result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.

NPM1
mutational
analysis
Containing thepreservation
solution(Container
capability of
5 mL)
Additives:
RPMI-1640
FBS
Kanamycin
sulfateNovo-heparin
sodiumSodiumbicarbonate
HEPES
Storageconditions:FreezeSAMPLE HANDLING METHOD

KIT
sequence analysis(leukemia)

Collect the specified amount ofsample, mix well, and refrigerate.
In this method, a result is affected
proportionally to the degree ofcontamination.
Therefore, pay careful attention whencollecting and handling samples.

Screeningof chimericgenes related
to leukemia,
quantitative
WT1 mRNAquantitativebone

bone marrow
fluid

Major BCR-ABL1 marrow (with theRmRNA,
fluid preservation
quantitative
1.0 each solution)
Major BCR-ABL1mRNA(％)

Shelf-life:
Use this after
Major BCR-ABL1
confirmingthat themRNA,
solution color qualitative
has turned to
Mutation
pale pink.
analysis in theThe color
turns to pale ABL1 region,
yellow after Major BCR-ABL1
freezing andminor BCR-ABL1
to pale pinkmRNA(％)
after
thawing.

1.0 eachObtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

container handling methodminor BCR-ABL1
mRNA,
quantitativeminor BCR-ABL1
mRNA, qualitative
micro BCR-ABL1
mRNA, qualitative
Mutation analysis
in the ABL1 region,
minor BCR-ABL1⑩

container handling method
CONTAINER FORM

H00
Previous container symbolH

container handling method⑪

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

TCF3-PBX1mRNA,Containing the quantitative
preservation
TCF3-PBX1
solutionmRNA,(Container
qualitative
capability of
5 mL)
PML-RARA
mRNA,
Additives:
quantitative
RPMI-1640
FBS
PML-RARA
KanamycinmRNA,sulfatequalitativeNovo-heparin
CBFB-MYH11
sodiummRNA,
Sodiumquantitative
Obtain 1.0 mL of bone marrow fluid
bicarbonatebone marrowinto a container shown in the left
HEPES
bone
fluid
CBFB-MYH11
image aseptically, mix well, andmarrow (with theRmRNA,
refrigerate the specimen.
Storagefluid preservation
qualitative
Please submit a sample on the day it
conditions:
1.0 each solution)is collected.
Freeze
1.0 each
RUNX1-RUNX1T1
mRNA,
Shelf-life:
quantitative
Use this after
RUNX1-RUNX1T1
confirmingmRNA,that thesolution color qualitative
has turned to
RUNX1-MECOM
pale pink.
mRNA,
The color
turns to pale qualitative
yellow after ETV6-RUNX1
freezing and mRNA,to pale pinkquantitative
after
ETV6-RUNX1
thawing.
mRNA,
qualitativecontainer handling method
CONTAINER FORM

H00
Previous container symbol

H

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD⑫

container handling method

KMT2A-AFF1
mRNA,
quantitative
KMT2A-AFF1
mRNA,
qualitative
KMT2A-AFDN
mRNA,
quantitative
KMT2A-AFDN
mRNA,
qualitative
KMT2A-MLLT3
mRNA,
quantitative
Containing the KMT2A-MLLT3
mRNA,
preservation
solution
qualitative(Container
KMT2A-MLLT1
capability of
mRNA,
5 mL)
quantitative
Additives:
KMT2A-MLLT1
RPMI-1640
mRNA,
FBS
qualitativeKanamycin
NUP98-HOXA9
sulfateNovo-heparin mRNA,
sodiumquantitativeSodium
STIL-TAL1
bicarbonatebone marrow
Obtain 1.0 mL of bone marrow fluid
mRNA,
HEPES
bone
fluid
into a container shown in the left
quantitative
marrow (with theimage aseptically, mix well, andR
Storagefluid preservation
refrigerate the specimen.
DEK-NUP214
conditions:
1.0 each solution)
Please submit a sample on the day itmRNA,Freeze1.0 eachis collected.
quantitative
Shelf-life:
DEK-NUP214
Use this after mRNA,
confirming
qualitativethat thesolution color T-cell receptor
has turned to β-chain Cβ1
rearrangement
pale pink.
The color
T-cell receptor
turns to paleβ-chain Jβ1yellow after
freezing and rearrangement
to pale pinkT-cell receptor
after
β-chain Jβ2
thawing.
rearrangement
T-cell receptor
γ-chain Jγ
rearrangement
T-cell receptor
δ-chain Jδ1
rearrangement
Immunoglobulin
H-chain JH
rearrangement
Immunoglobulin
H-chain Cμrearrangement
Immunoglobulin
L-chain Jκrearrangementcontainer handling method
CONTAINER FORM

H00

Containing thepreservation
Previous container symbol
solution
H(Container
capability of
5 mL)
Additives:
RPMI-1640
FBS
Kanamycin
sulfateNovo-heparin
sodiumSodiumbicarbonate
HEPES
Storageconditions:FreezeTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Immunoglobulin
L-chain Cκrearrangement
Immunoglobulin
L-chain Cλrearrangement
Chimerism
analysis,
pre-transplant,
recipient, PCR

Chimerism
analysis,
pre-transplant,
Shelf-life:
donor, PCR
Use this after

Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
image aseptically, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.
bone marrow
bone
fluid
marrow (with thefluid preservation
1.0 each solution)
1.0 eachRumbilical umbilical cord
cord blood (with thebloodpreservation
1.0
solution) 1.0

confirmingthat thesolution color
has turned topale pink.
The color
Chimerismbone
bone marrow
turns to pale analysis,
marrow fluid (with theyellow after post-transplant,
fluidpreservation
freezing and PCR
1.0
solution) 1.0
to pale pinkafter
thawing.

container handling method⑬

SAMPLE HANDLING METHOD

Collect the specified amount ofsample, mix well, and refrigerate.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

container handling method
CONTAINER FORMTEST NAME

Containing thepreservation
Previous container symbol solution(Container
H2capability of
10 mL)

Malignantlymphomaanalysis (MLA),
CD45 gating,test forhematopoietic
malignant tumor
cell

H20

Additives:
FBS
PBS
Kanamycin
sulfateStorageconditions:FreezeShelf-life:
1 year

CONTAINER FORM

Malignantlymphomaanalysis,
7AAD analysis,
test forhematopoietic
malignanttumor cells

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

lymph nodelymph(with thenodepreservation
5×5×5
solution)mm
5×5×5 mmeach
each

Place lymph nodes 5×5×5 mm in sizein a container shown in the left image,R suspend, and refrigerate.
Please submit a sample on the day it
is collected.

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Containing
deproteinization
Previous container symbol solution(Content volumer3
Lactic acidof 1 mL)

SAMPLE HANDLING METHODSAMPLE HANDLING METHOD

M30

Additives:
0.8 N
perchloric
acid
Storageconditions:
Refrigeration

whole
blood
1.0
each

Immediately

Deproteinizedsupernatant
0.4
each

Immediately after blood sampling,
pipette accurately 1.0 mL of blood
into the dedicated container. AfterR
stirring thoroughly, centrifuge thetube at 3000 rpm for 5 minutes, andsubmit the supernatant.

Pyruvic acid

Shelf-life:
1 year

CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

M40

Containing
deproteinization
Previous container symbol solution(Content volumer4
of 4 mL)
Additives:
Sodiumtungstate,sulfuric acid

Immediately

Deproteinizedsupernatant
3

F

Immediately after blood sampling,
pipette accurately 1.0 mL of blood
into the dedicated container. After
stirring thoroughly, centrifuge thetube at 3000 rpm for 5 minutes, andsubmit the supernatant.

container handling method

Storageconditions:
Refrigeration

Ammonia

whole
blood
1.0

Shelf-life:
1 year⑭

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Containing
deproteinization
Previous container symbol solution(Content
r5
volume of
0.5 mL)SAMPLE HANDLING METHOD

M50

Additives:
0.8 N
perchloric
acidLight Shielding

Vitamin C(ascorbic acid)

serum
0.5

deproteinizedsupernatant
0.5

F

Storageconditions:
Refrigeration

Immediately after blood sampling,
separate serum, and pipetteaccurately 0.5 mL of serum into thededicated container. After stirring
thoroughly, centrifuge the tube at
3000 rpm for 5 minutes. Transfer thesupernatant into a light-shielding
polyethylene test tube (ASS), freeze,
and submit the specimen under frozen
conditions.

Shelf-life:
1 year

CONTAINER FORM

PAC
Previous container symbol

B

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Containing
ACD-A solution
Additives:
ACD-A
solution
Storageconditions:Roomtemperature

PlateletassociatedImmunoglobulin
G (PAIgG )

whole
blood
7.5

Shelf-life:
1 year
(1 month after
opening thealuminumpackage)

CONTAINER FORM

PAP
Previous container symbol

D

Containing
EDTA-2Na andaprotinin (3mL
vacuum blood
sampling tube)

container handling method

Additives:
EDTA-2Na
3.75 mg
Aprotinin(1500 units)
Storageconditions:Roomtemperature
Shelf-life:
1 year⑮

SAMPLE HANDLING METHODTEST NAME

Human atrial
natriuretic
peptide (HANP)

After drawing the specified amount ofblood, inject the blood into a container
shown in the left image. After mixing
well, refrigerate the specimen.
R(Note) If peripheral platelet count is ≤3 × 104/μL, use two dedicated tubes
to surely collect at least 10 mL ofblood.

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Parathyroid
hormone related
peptide (PTHrP)

Pancreaticglucagon

whole
blood
(with
ACD-A
solution)
7.5

whole
blood
1.5–2.0
each

Refrigerated

plasma0.5

Immediately
Refrigerated

plasma0.5

Immediately
Refrigerated

plasma0.5

SAMPLE HANDLING METHOD
After drawing blood into a container
shown in the left image, mix well, andseparate plasma at low temperature(4℃ ).
Be sure to freeze the plasma sample
for storage.

F

Draw blood into a container shownin the left image, mix well, separate
plasma at low temperature (4℃ ), andfreeze the specimen immediately.

Draw the specified amount of blood
into a container shown in the left
image in resting conditions, mixwell, and separate plasma at low
temperature (4℃ ).
Be sure to freeze the plasma sample
for storage.

container handling method
CONTAINER FORMTEST NAMESTORE
AMOUNT COLLECTED AMOUNT SUBMITTED TEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHODPARPrevious container symbol

B1

Containing
ACD-A solution
Additives：
ACD-A
solution
Storageconditions：Roomtemperature

Flow cytometry
crossmatch,
lymphocyte
crossmatch test

whole
blood
7.5

whole
blood (with
ACD-A
solution)
7.5

R

After drawing the specified amount ofblood, inject the blood into a container
shown in the left image. After mixing
well, and store at room temperature.

Shelf-life:
1 year(1 monthafter opening
the aluminum
package)

CONTAINER FORM TEST NAMESTORETEMPERATUREPBTSAMPLING METHOD
Cool the dedicatedcontainer（PBT）in
advance.

Previous container symbol

Q

Make sure that thesurface of specimen in
the dedicated container isbelow the surface of the icewater.
Do not use ice cubes.

use any vacuum blood
Collect 3.0 mL of blood sample Do not 、
sampling tube, catheter, or otherin a plastic syringe with a、、
20 gauge needle, if possible、 sampling methods. Also, do、
not（19–21 gauge needle is also
、 use any tourniquets. Avoid
acceptable）.（Do not collect、 drawing ≥10 mL of blood. Collect、blood samples directly using a blood smoothly while avoiding
any damage to the vascular walls.
dedicated container [PBT].）βthromboglobulin(β-TG)、

Removethe needle and gently、
open the、cap of the dedicated Use only the dedicated、container.
container（PBT）to gently
transfer 2.7 mL of the blood Do not shake the dedicatedcontainer.
sample into the tube. Invert
the tube slowly 2 to 3 times.

F

Additives:
Theophylline
Adenosine
Dipyridamole Platelet
Sodiumfactor-4
citrate(PF-4)
Citric acid
Storageconditions:
Refrigerationand protection
from light
Shelf-life:
1 yearMake sure that the surfaceof blood in the dedicatedcontainer is below thesurface of the ice water.

、
Proceed to the following procedures
within 2 minutes.

After allowing the tube to stand、in a
crushed ice-water mixture for 15 to 30
Make sure to centrifuge
minutes, centrifuge the tube at 2000 g
within 1 hour under coolfor 30 minutes at 2 to 4℃ .（The tableconditions.
below shows the rotor radius and the
speed [rpm] at 2000 G.）*Equation of therotating speed of the centrifuge
Conversion table
G = 1.118 × 10−5× r × n2
RadiusSpeed Radius Speed
r:radius of the centrifuge rotor（cm）
n:number of revolutions per minute（rpm） （cm） （rpm） （cm） （rpm）
10
4200
22
2800
Collect 0.3 mL of supernatant per testing
12
3800
24
2700
sample from a little lower than the surface
into a specimen container using a micropipette.
14
3500
26
2600
Do not collect the supernatant close to
16
3300
28
2500
the pellet.
18
3100
30
2400
（Avoid collecting all plasma.）
20
3000
Make sure to freeze the specimen（stability for 1 month）.

⑯

container handling method(Do not use avacuum tube.)Containing
antiplatelet
agent

Promptly place thededicated container in a
rack with crushed ice andwater.

container handling method
CONTAINER FORM

PC2TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)
Immediately

plasma0.3

Immediately

plasma0.5
each

Soluble fibrin monomer
complex (SFMC)

Immediately

plasma0.4

Fibrin monomer
complex, quantitative

Immediately

plasma0.5

Immediately

plasma0.3

Immediately

plasma0.5

Immediately

plasma0.3

HeparinPrevious container symbol

Activated partial
thromboplastin time
(APTT)

K

Prothrombin time(PT)

SAMPLE HANDLING METHOD

Fibrinogen
(FIB)

FDP,
Containing
3.2% sodium
quantitative
citrate(1.8mL vacuum
blood sampling D-dimertube)
Additives:3.2% sodium
citrate
0.2 mL
Storageconditions:Roomtemperature
Shelf-life:
1 year
(1 month after
opening theplastic casethat contains
25 tubes)

container handling method⑰

Prothrombin
fragment F1+2
Antithrombin III(AT-III)
Thrombin/
Antithrombin
complex III (TAT)

whole
blood
1.8
each

Draw 1.8 mL of blood into a tube
containing 0.2 mL of 3.2% sodium
citrate, invert the tube 5 to 6 times,
and promptly separate plasma.
Be sure to freeze the plasma sample
for storage.
(When submitting ≥1.8 mL of blood
with multiple test requests, use thePC5 container.)F

Immediately

plasma0.5
each

Plasmin-α2-plasmin
inhibitor complex(PIC test)

Immediately

plasma0.2

Total PAI-1(tPA/PAI-1
complex)

Immediately
Refrigerated

plasma0.5

Factor Ⅷ(F8),Chromogenic
Substrate
Coagulation methodfactoractivity test Factor Ⅸ(F9),Chromogenic
Substrate
methodImmediately

plasma0.3
each

Plasminogen
Antiplasmin (α2plasmin inhibitor)[Points to note]* Please be advised of the following
procedures for total PAI-1.
1. Use the PC2 container for sampling,
and be sure to centrifuge blood
under refrigerated conditions.
Avoid centrifugation at room
temperature or using the PC5
container, because such procedures
may cause increasing the values.
2. If the sample cannot be separated
immediately, put it in ice water andseparate plasma within 1 hour.

container handling method
CONTAINER FORM

PC2TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

FactorII (F2)Previous container symbol

FactorV (F5)

K

FactorVII (F7)
Factor
VIII (F8)

Coagulation
Factorfactoractivity test IX (F9)FactorX (F10)
Containing
3.2% sodium
citrate(1.8mL vacuum
blood samplingtube)
Additives:3.2% sodium
citrate
0.2 mL
Storageconditions:Roomtemperature
Shelf-life:
1 year
(1 month after
opening theplastic casethat contains
25 tubes)

Immediately

plasma0.4
each

Immediately

plasma0.2

Immediately

plasma0.5

Immediately

plasma0.4

Immediately

plasma0.3

Immediately

plasma0.6

Protein C(antigen level)

Immediately

plasma0.3

Protein C
activity

Immediately

plasma0.4

Protein S(antigen level)

Immediately

plasma0.2

Protein S
activity

Immediately

plasma0.4

Protein S(free antigen level)

Immediately

plasma0.2

Thrombomodulin

Immediately

plasma0.4

FactorXI (F11)
FactorXII (F12)
FactorXIII (F13)
von Willebrandfactor antigen assay
whole
von Willebrandblood
factor activity
1.8(ristocetin cofactor) eachvon Willebrandfactor multimer
analysis
ADAMTS13
activity
ADAMTS13
inhibitor

F

Draw 1.8 mL of blood into a tube
containing 0.2 mL of 3.2% sodium
citrate, invert the tube 5 to 6 times,
and promptly separate plasma.
Be sure to freeze the plasma sample
for storage.
(When submitting ≥1.8 mL of blood
with multiple test requests, use thePC5 container.)

container handling method⑱container handling method
CONTAINER FORM
Containing
3.2% sodium
Previous container symbol citrate(1.8mL vacuumKblood sampling
tube)

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

PC2Additives:3.2% sodium
citrate
0.2 mL
Storageconditions:Roomtemperature
Shelf-life:
1 year
(1 month after
opening theplastic casethat contains
25 tubes)

CONTAINER FORM

HIT antibody(Platelet factor
4-heparin
complex
antibody)

Immediately

plasma
0.5

whole
blood
1.8
each

C1 inactivator
activity(C1 esterase
inhibitor
activity)

TEST NAME

Draw 1.8 mL of blood into a tube
containing 0.2 mL of 3.2% sodium
citrate, invert the tube 5 to 6 times,
and promptly separate plasma.
Be sure to freeze the plasma sample
for storage.

F

Immediately

Draw 1.8 mL of blood into a tube
containing 0.2 mL of 3.2% sodium
citrate, invert the tube 5 to 6 times,
and promptly separate plasma.
Be sure to freeze the plasma sample
for storage.
(When submitting ≥1.8 mL of blood
with multiple test requests, use thePC5 container.)

plasma0.2

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

PC5
Previous container symbol

L

Containing
3.2% sodium
citrate(4.5mL vacuum
blood samplingtube)
Additives:3.2% sodium
citrate
0.5 mL

Light Shielding

Vitamin K
fractionation

FactorCoagulation VIII
inhibitor
identification Factor
IX

Storageconditions:Roomtemperature

container handling method

Shelf-life:
1 year
(1 month after
opening theplastic casethat contains
25 tubes)⑲

Lupusanticoagulant

Immediately

plasma2.0

Immediately

plasma
1.0
each

whole
blood
4.5
each

F

Immediately

plasma1.0

SAMPLE HANDLING METHOD
Draw 4.5 mL of blood into a tube
containing 0.5 mL of 3.2% sodium
citrate, invert the tube 5 to 6 times,
and promptly separate plasma.
Be sure to use the“light-shielding
polyethylene test tube (ASS)”whensubmitting plasma samples.
Be sure to freeze the plasma sample
for storage.
Draw 4.5 mL of blood into a tube
containing 0.5 mL of 3.2% sodium
citrate, invert the tube 5 to 6 times,
and promptly separate plasma.
Be sure to freeze the plasma sample
for storage.
Centrifuge at ≥ 1500 G for 15 minutes atroom temperature immediately after bloodis drawn, take plasma at the position of ≥5 mm above the buffy coat, and submit itunder frozen conditions.
Handle the samples with care to avoid
platelet contamination which may affectthe results.
*Equation of the rotating speed of thecentrifuge
G＝1.118×10-5×r×n2
r: radius of the centrifuge rotor (cm)n: number of revolutions per minute (rpm)container handling method
TEST NAMECONTAINER FORM

Containing
EDTA and cellPrevious container symbol preservative
RAS gene(8.5mL vacuum mutationalPdblood sampling
analysistube)

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

PCD〔BEAMing〕

whole blood(with EDTA andcell preservative)
8.5

Additives:
EDTA and cellpreservative
Storageconditions:Roomtemperature
Shelf-life:
18 months

CONTAINER FORM

PCF

FoundationOne
Liquid CDx
Cancer genome
profileTEST NAME

whole blood(with EDTA andcell preservative)
17.0×2After drawing blood into a container
shown in the left image, mix well, andR store at room temperature. Pleasesubmit a sample on the day it iscollected.

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

Containing
Cell-Free DNA
BCT CE(10mL vacuum
blood samplingtube)

RAS gene
Additives:
Cell-Free DNA mutational
BCT CE
analysis
Storageconditions:Roomtemperature〔BEAMing〕

whole blood(with Cell-Free DNA
BCT CE)
10.0

After drawing blood into a container
shown in the left image, mix well, andR store at room temperature. Pleasesubmit a sample on the day it iscollected.

Shelf-life:
2 years

CONTAINER FORM
(2mL vacuum
blood sampling
Previous container symbol tube)

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

PE2
T3

Endotoxin,

quantitative
Additives:
Novo-heparin
15 IU

Shelf-life:
1 yearβ-D-glucan

R

After drawing blood into a container
shown in the left image, mix well.
Promptly refrigerate the specimen.
The data will be affected if it is kept
at room temperature.
Avoid putting in another request at
the same time, except for β-D-glucan.
After drawing blood into a container
shown in the left image, mix well.
Promptly refrigerate the specimen.
Avoid putting in another request at
the same time, except for Endotoxin
quantitative.

⑳

container handling method

Storageconditions:Roomtemperature

whole blood(with Novo-heparin)
2.0 each

SAMPLE HANDLING METHOD

container handling method
CONTAINER FORMTEST NAMEPE4

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLING METHOD
1SolutionA

Previous container symbol

T4

Solution
B

Endotoxin in
dialysis fluid(4.0mL vacuum
blood sampling tube)

2
Aseptically collectdialysis fluid using aneedle-attachedsyringe, and inject
into the PE4 container
by pushing the needle
through the rubber
stopper.

Dialysis fluidRO(containing stabilizer) R
water
4.0 eachPreparation
solution

3

Additives:
Stabilizer 0.1 mL
Storage conditions:
Refrigeration

othersShelf-life: 1 year

CONTAINER FORMTEST NAME

Containing sodium
fluoride
Previous container symbol (2mL vacuum blood Glucose
sampling tube)

PF2
E

Additives:
Sodium fluoride
2.5 mgHeparin sodium
25 uspu
EDTA-2Na
7.4 mgStorage conditions:
Room temperature
Shelf-life:
2 years

container handling method�

Pull up the color cap of
the PE4 container towards
the arrow direction to
open while keeping oneedge of the cap on thealuminum part (so thatthe cap stays on the topedge). Clean the rubber
stopper with an alcohol
disinfection cotton.

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

whole
blood
1.5–2.0

plasma0.5

After injection, cover
the color cap to theoriginal state.
Promptly shake thecontainer about 4 times
to mix the stabilizer
uniformly. Promptly
refrigerate, and submit
the specimen.

SAMPLE HANDLING METHOD
After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

Hemoglobin A1cwhole blood
After drawing blood into a container(with sodium fluoride) R shown in the left image, mix well, and(HbA1c)2.0
refrigerate the specimen.
(NGSP)

Glycoalbumin

whole
blood
2.0

plasma0.5

After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

container handling method
CONTAINER FORM

PH5

TEST NAME
γ-aminobutyricacid (GABA)

Previous container symbol

G

Fatty acid
fractionation(24)
Fatty acid
fractionation (4)Very long chain
fatty acid

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

whole
blood
3.0

Immediately

plasma1.0

FContaining
Nicotinic acidheparin(niacin)(5mL vacuum
blood samplingtube)
Additives:
Heparinsodium65IU
Storageconditions:Roomtemperature
Shelf-life:
2 years

After drawing blood into a container
shown in the left image, mix well, andseparate plasma immediately.
Be sure to freeze the plasma sample
for storage.

whole
blood
1.5–2.0
each

plasma0.5 each

Draw blood into a container shown in
the left image under fasted conditions
R in the early morning, mix well, andseparate plasma.
Refrigerate the plasma samples.

whole
blood
4.5–5.0

plasma2.0

After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Freeze the plasma samples for storage.
F

Lipoprotein
lipase (LPL)

SAMPLE HANDLING METHOD

whole
blood
1.5–2.0

Immediately
Refrigerated

plasma0.3

Provide a patient with 30 units of heparin
per kilogram of body weight intravenously
under fasted conditions in the early morning.
15 minutes later, draw blood into a container
shown in the left image, and centrifuge the blood
at low temperature (4℃ ).
Be sure to freeze the plasma sample for storage.

whole blood(with heparin)
1.5

Lead (Pb)

whole blood(with heparin)
3.0

Chromiumwhole blood(with heparin)
0.7

Cadmium

whole blood(with heparin)
0.5

Manganesewhole blood(with heparin)
0.7

Ethanolwhole blood(with heparin)
1.0

R

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.

F

After drawing blood into a container
shown in the left image, mix well.
Transfer the blood into a polyethylene
test tube, and freeze the specimen.

R

After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

plasma0.3

Voriconazole

Ethosuximide
Phenobarbital
Phenytoin

whole
blood
1.5–2.0
each

plasma0.5 eachPrimidone

plasma0.3

Valproateplasma0.5

�container handling method

Carbamazepine

container handling method
CONTAINER FORM

PH5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

Gabapentin

Previous container symbolG

Lamotrigine
Topiramate
Levetiracetam
Stiripentol
plasma0.3 each

Perampanel
Lacosamide
Containing
Amiodarone
heparin(5mL vacuum
blood sampling
Bepridiltube)
Additives:
Heparinsodium65IU
Storageconditions:Roomtemperature
Shelf-life:
2 years

whole
blood
1.5–2.0
each

Aprindine

After drawing blood into a container
shown in the left image, mix well, andR
separate plasma.
Refrigerate the plasma samples.

Disopyramide
plasma0.4

Procainamide
Propranolol

plasma0.3 eachPilsicainide
Cibenzoline
Pirmenolwhole blood
3.0–4.0

plasma0.4

Quinidinecontainer handling method

Flecainide
Theophylline
Methotrexate�

plasma
1.3

whole
blood
1.5–2.0
each

plasma0.3

plasma0.5 eachcontainer handling method
CONTAINER FORM

PH5

TEST NAME
CCR4 protein, FCM

Previous container symbol

G

Tuberculosis
specific
IFN-γ

Shelf-life:
2 years

plasma0.4

Presepsin

CD34,
quantitative

whole blood(with heparin)
5.0

Erythrocyte surface
marker analysis CD59
Leukemia/lymphoma analysis (LLA),
CD45 gating, test for hematopoietic
Containing
malignant tumor cells
heparin(5mL vacuum
Malignant lymphoma analysis
blood sampling (MLA), CD45 gating, test for
tube)
hematopoietic malignant tumor cells

Storageconditions:Roomtemperature

whole blood(with heparin)
5.0 each

whole
blood
1.5-2.0

Erythrocyte surface
marker analysis CD55

Additives:
Heparinsodium65IU

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

T cell percentage
B cell percentage

Immediately

whole blood(with heparin)
1.0 eachwhole blood(with heparin)
5.0 each

IgG-FcR+/T-cell
percentage
Platelet surface
marker analysis CD41
Platelet surface marker
analysis CD42b
Th1/Th2(IFN-γ×IL-4/CD4)

After drawing blood into a container shown in theleft image, gently invert the tube 2 to 3 times, andseparate plasma immediately.
Be sure to freeze the plasma sample for storage.
Avoid excessive mix by a vortex mixer or others,
mild stirring for a long time, or freeze-thawing
method that may cause the elevated values.
Avoid putting in another request at the same time.
After drawing blood into a container shown in theleft image, mix well, and store at room temperature.
Freeze the sample containing DMSO.
Avoid putting in another request at the same time.
Please submit a sample on the day it is collected.

After drawing blood into a container
shown in the left image, mix well, andstore at room temperature.
Please submit a sample on the day it
is collected.

After drawing blood into a container shown in the left
image, mix well, and store at room temperature (17–25℃).
Draw a larger amount of blood from patients with lowlevels of lymphocytes.
Please submit a sample on the day it is collected.

whole blood(with heparin)
1.0 each

After drawing blood into a container
shown in the left image, mix well, andstore at room temperature (17–25℃ ).
Draw a larger amount of blood from
R patients with low levels of
lymphocytes.
(3.0 mL when requesting ≥3 test items)
Please submit a sample on the day it
is collected.

whole blood(with heparin)
3.0 each

After drawing blood into a container
shown in the left image, mix well, andstore at room temperature.
(Collect 5.0 mL of blood whenrequesting at least 10 items.)
Please submit a sample on the day it
is collected.

whole blood(with heparin)
1.0

After drawing blood into a container shown in the left
image, mix well, and store at room temperature (17–25℃).
Draw a larger amount of blood from patients with lowlevels of lymphocytes.
Please submit a sample on the day it is collected.

whole blood(with heparin)
5.0 eachwhole blood(with heparin)
3.0

After drawing blood into a container
shown in the left image, mix well, andstore at room temperature.
Please submit a sample on the day it
is collected.

�container handling method

K
L
Lymphocyte surface
markerautomated analysis bymonoclonal antibodies
Lymphocyte
surface marker
analysis by twocolor analysis

F

After drawing blood into a container shown in the left
image, mix well, and store at room temperature (18–25℃).
Please submit a sample on the day it is collected.

whole blood(with heparin)
3.0

IgG
IgA
B-cell surface
immunoglobulin IgM(Sm-Ig)
IgD

R

SAMPLE HANDLING METHOD
After drawing blood into a container shown in theleft image, mix well, and store at room temperature.
Please submit a sample on the day it is collected.

container handling method
CONTAINER FORMTEST NAME
Cytomegalovirusspecific CTL assay(HLA-A*0201)
Cytomegalovirusspecific CTL assay(HLA-A*2402)

PH5
Previous container symbol

G

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

whole blood(with heparin)
5.0 each

After drawing blood into a container
shown in the left image, mix well, andR store at room temperature.
Please submit a sample on the day it
is collected.

whole blood(with heparin)
3.0 each

After drawing blood into a container
shown in the left image, mix well, andR refrigerate the specimen.
Please submit a sample on the day it
is collected.

G-BandingCongenital C-Banding
chromosomal
anomalies Q-Banding
High-resolution
banding
Chromosome analysis using spectral
karyotyping (SKY) (Congenital anomaly)
Fragile X chromosome(Fragile X syndrome)
Chromosome 1(1p36 deletion syndrome)
Containing
heparin
Chromosome 4(5mL vacuum
blood sampling (Wolf-Hirschhorn syndrome)tube)
Additives:
Heparinsodium65IU
Storageconditions:Roomtemperature
Shelf-life:
2 years

Chromosome 5(Sotos syndrome)
Chromosome 7(Williams syndrome)
Congenital chromosomal
anomaly, Chromosome 13

Chromosome 13(Congenital retinoblastoma, RB1)
Chromosome 15(Prader-Willi syndrome)
Chromosome 15(Angelman syndrome)
Chromosome 17(Miller-Dieker syndrome)

container handling method

Congenital chromosomal
anomaly, Chromosome 18
Congenital chromosomal
anomaly, Chromosome 21
Chromosome 22 (22q11
deletion) (CATCH22)
Conotruncal anomaly face
syndrome
Velo-cardio-facial syndrome
DiGeorge syndrome
Congenital chromosomal
anomalies Chromosome X
Chromosome X (Steroid
sulfatase [STS] gene)�container handling method
CONTAINER FORMTEST NAME
Congenital
chromosomal anomalies
Chromosome Y
Chromosome Y(Sex-determining
region Y [SRY])
Chromosomes XY(Short stature
homeobox [SHOX])
Chromosome anomaly
associated withhematological disorders,
G-Banding

PH5
Previous container symbol

G

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

whole blood(with heparin)
3.0 each

Chromosome analysis using
spectral karyotyping (SKY)(Hematological disorder)
TCF3-PBX1 t(1;19)translocation

Containing
heparin
CKS1B 1q21
(5mL vacuum
amplification
blood sampling
tube)
ALK 2p23
Additives:
Heparinsodium65IU
Storageconditions:Roomtemperature
Shelf-life:
2 yearstranslocation

After drawing blood into a container
shown in the left image, mix well, andR refrigerate the specimen.
Please submit a sample on the day it
is collected.

GATA2MECOM inv(3)inversion, t(3;3)
translocation
BCL6 3q27translocation
IGH-FGFR3 t(4;14)translocation

whole blood(with heparin)
5.0 each

FIP1L1-PDGFRA
4q deletion(4q12 deletion)
CSF1R 5q
deletion
EGR1 5q
deletion
PDGFRB 5q32
translocation

container handling method

D7S486 7q deletion/
Chromosome 7monosomy
Chromosome
anomaly associatedwith hematologicaldisorders,
Chromosome 8�container handling method
CONTAINER FORM

PH5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

MYC 8q24translocation

Previous container symbol

IGH-MYC t(8;14)translocation

G

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

RUNX1-RUNX1T1(AML1-MTG8)t(8;21) translocation
FGFR1 8p11.2
translocation
BCR-ABL1 t(9;22)translocation

After drawing blood into a container shown in the left
image, mix well, and refrigerate the specimen.
Submit the specimens promptly after blood sampling.
If more than 30 hours has passed from sample collection,
the sample cannot be used for the test due to cellularmorphological changes which may affect the test result.

Peripheral bloodneutrophils
BCR-ABL1t(9;22)translocation
KMT2A (MLL)
11q23.3translocation
IGH-CCND1(IGH-BCL1)t(11;14)translocation

Containing
heparin(5mL vacuum
blood sampling NUP98 11p15
tube)

translocation

Additives:
Heparinsodium65IU
Storageconditions:Roomtemperature
Shelf-life:
2 years

BIRC3-MALT1 (API2MALT1) t(11;18)translocation
ATM 11q
deletion
Chromosome
anomaly associatedwith hematologicaldisorders,
Chromosome 12
ETV6-RUNX1 (TELAML1) t(12;21)translocation
D13S319 13q
deletioncontainer handling method

IGH-BCL2 t(14;18)translocation
IGH-MAF t(14;16)translocation
PML-RARA t(15;17)translocation
CBFB inv(16) inversion,t(16;16) translocation
TP53 17p
deletion
MALT1 18q21translocation
BCL2 18q21translocation

�whole blood(with heparin)
5.0 eachR

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

container handling method
CONTAINER FORM

PH5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

20q deletion

Previous container symbol

G

Containing
heparin(5mL vacuum
blood samplingtube)
Additives:
Heparinsodium65IU

Chromosome
anomaly associatedwith hematologicaldisorders,
Chromosome X
Chromosome
anomaly associatedwith hematologicaldisorders,
Chromosome Y
Sex-mismatched bonemarrow transplantation(BMT) (Chromosomes XY)

R

Storageconditions:Roomtemperature
Shelf-life:
2 years

FLT3 mutational
analysis ITD/
TKD

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.

whole blood(with heparin)
5.0 eachwhole blood(with heparin)
3.0

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request
at the same time. In this method, aresult is affected proportionally to thedegree of contamination.
Therefore, pay careful attention whencollecting and handling samples.

container handling method�container handling method
CONTAINER FORMTEST NAME
Lymphocyte
blastoidtransformation
by PHA

PH9
Previous container symbol

I

Lymphocyte
blastoidtransformation
by Con-A

Containing
heparin(10mL vacuum
blood sampling
Drug-induced
tube)
Additives:
Heparin
sodium
130IU

lymphocyte
stimulation test
(DLST)AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

whole blood(with heparin)
5.0 each

1 drugwhole blood(with heparin)
12.0

Collect 2 tubes of blood per drug inthe designated container, mix well,and be sure to store at room
temperature.
1. Each additional drug requires 5.0
mL of blood.
2. One drug requires 5 million
lymphocytes. In some cases, tests
may not be conducted due toinsufficient lymphocytes.
3. As a guide, double the amount ofblood collected when the leukocyte
count is ≤ 3000 mm3. Please contact
our sales in charge if you have theleukocyte count and the percentage
of lymphocytes in detail.
4. When you place an order for 2 ormore items, please specify priority.
5. Please submit a blood sample
together with a drug you would
like to test. Tablets (one tablet),capsules (one capsule), powders
R(one packet of approx. one dose),
liquid-type internal medicines(approx. 0.5 mL), injection
medicines (one vial [ampule]).
We will not be able to conduct
the test with an ampule for an
intradermal reaction test as it
contains a trace quantity. If you
wish to use other drugs, pleasecontact our sales in charge.
6. If heparin is suspected to be a
responsible agent, please collect
all blood in the dedicated container(PNK) and submit it.
Please submit a sample on the day
it is collected.

whole blood(with heparin)(Recipient)
20.0(donor)10.0(unrelated donor)
10.0

Draw 20.0 mL (×2) of blood from arecipient, 10.0 mL (×1) of blood froma donor, and blood 10.0 mL (×1) ofblood from an unrelated donor into a
container shown in the left image, mixwell, and store at room temperature.
Do not open the container or transfer
samples. MLC is necessary to keep
lymphocytes cultured in sterile
conditions.
Fill name of institution and subject
name on the container label.
Draw a larger amount of bloodfrom patients with low levels of
lymphocytes.

Storageconditions:Roomtemperature
Shelf-life:
2 years

container handling method

Mixedlymphocyte
culture(MLC)�SAMPLE HANDLING METHOD
After drawing blood into a container
shown in the left image, mix well, andstore at room temperature (17–25℃ ).
Draw a larger amount of bloodfrom patients with low levels of
lymphocytes.
(8.0 mL when requesting the 2 test
items at the same time)
Please submit a sample on the day it
is collected.

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHODPHSContaining
heparinLight Shielding
Previous container symbol (4mL vacuum blood
G3
sampling tube)
Coproporphyrin
Additives:
Heparinsodium
83.6 units
Storageconditions:RoomtemperatureLight Shielding

Uroporphyrin

whole blood(with heparin)
1.5 each

After drawing blood into alight-shielding container shown in theleft image, mix well, and refrigerateR
the specimen.
Be sure to use the light-shielding
container.

Shelf-life:
Light Shielding
18 months(1 month afterProtoporphyrin
opening thealuminum package)

CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

PK2Previous container symbolgContaining
Peripheral blood
EDTA-2K
general test
(2mL vacuum
blood samplingtube)
Additives:
EDTA-2K
3.8 mgStorageconditions:Roomtemperature
Shelf-life:
2 years

whole blood(with EDTA-2K)
2.0 each

After drawing blood into a container
shown in the left image. Immediately
after collecting, gently mix the bloodby inverting the tube ≥ 5 times, andrefrigerate the specimen.
We cannot test hemolytic, coagulated,or frozen samples.
R Please submit a sample on the day it iscollected.

Reticulocyte
count
Eosinophil
granulocyte countComplete bloodcount (CBC)�

container handling method

NCC Oncopanel
SystemCollect blood sample in the dedicatedcontainer, mix well, and refrigerate
the specimen. After the specimen
collection, please submit it within 14days.

container handling method
CONTAINER FORM

PK5
PK7

For 5mL（PK5）

TEST NAME

Containing
EDTA-2K(5mL or 7mL
vacuum blood
sampling tube)
Contents:
EDTA-2K
9.5 mg or
13.3 mg

EGFR mutation
analysis v2.0
(plasma)

Storageconditions:Roomtemperature
For 7mL（PK7）

PKF

plasma5.0

F

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Containing
EDTA-2K(2mL vacuum
Light Shielding
blood sampling
Vitamin
B2
tube)
Additives:
EDTA-2K
3.6 mgStorageconditions:Roomtemperature

whole blood(with EDTA-2K)
0.5 each

Cyclosporine

Tacrolimus

Everolimus

Draw a sufficient volume of blood to
obtain the required specimen (5mL ofplasma) into a tube shown in the left
image. After blood sampling, makesure to separate plasma within 4hours.
Immediately after the separation,
pipette 2.5 mL each of plasma into 2
sterile polyethylene test tubes (ARR)
and store them frozen.
Decanting is not permitted whenobtaining the plasma aliquots to avoid
contamination with leukocyte-derived
genomic DNA.
Avoid putting in another request at
the same time. In this method, theresult is affected proportionally to thedegree of contamination. Therefore,
pay careful attention when collecting
and handling samples.

SAMPLE HANDLING METHOD

Draw blood into the designated container, andmix well. Transfer the blood into a light-shielding
polyethylene test tube (ASS), submit the
specimen under frozen conditions.

Fwhole blood(with EDTA-2K)
0.7 each

Shelf-life:
1 yearSAMPLE HANDLING METHOD

After drawing blood into a container
shown in the left image, mix well, andfreeze the specimen.

Vitamin B1

Previous container symbol

container handling method

�

whole
blood
10.014.0

Shelf-life:
2 years

CONTAINER FORM

g1

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

whole blood(with EDTA-2K)
1.0

After drawing blood into a container
shown in the left image, mix well, andfreeze the blood.
Please note that this cannot berequestedsimultaneously with other test items.

container handling method
CONTAINER FORM

PN2
PN5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Pancreatic
phospholipase A2(pancreatic PLA2)Previous container symbolC

Refrigerated

Amino acid analysis,
39 types, LC/MSF

Amino acid analysis,
9 types, LC/MS

Containing
EDTA-2Na(2 or 5mL
vacuum blood
sampling tube)

For 2 mL
(PN2)

plasma0.3

Amino acid analysis,
2 types (tyrosine/
phenylalanine),
LC/MS
Homocystein,
total

Storageconditions:Roomtemperature
Shelf-life:
2 years

Imatinib

Adrenocorticotropic
hormone (ACTH)

Immediately

plasma0.3

plasma0.3

Rufinamide

Additives:
Mycophenolic
EDTA-2Na
3.0 or 7.5 mg acid

Immediately
Refrigerated

plasma
0.5
each

whole
blood
1.5–2.0
each

plasma0.5

RAfter drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

F

Promptly after drawing blood into
a container shown in the left image
in resting conditions in the earlymorning, mix well, and separate
plasma at low temperature (4℃ ).
Be sure to freeze the plasma sample
for storage.

Immediately
Refrigerated

plasma0.5

RAfter drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

F

Draw blood into a container shown in
the left image in resting conditions in
the early morning, mix well, andseparate plasma.
Freeze the plasma samples for storage.

plasma0.5 eachCortisol

Promptly after drawing blood into
a container shown in the left image,mix well, and separate plasma at low
temperature (4℃ ).
Be sure to freeze the plasma sample
for storage.

plasma0.3

Parathyroid
hormone(whole PTH)

For 5 mL
(PN5)

SAMPLE HANDLING METHOD
After drawing blood into a container
shown in the left image, mix well, andseparate plasma at low temperature(4℃ ).
Be sure to freeze the plasma sample
for storage.

container handling method�container handling method
CONTAINER FORM

PN2
PN5

TEST NAME
Serotonin

Previous container symbol

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD
After drawing blood into a container
shown in the left image, mix well.
Transfer the blood into a polyethylene
test tube, and freeze the specimen.

whole blood(with EDTA-2Na)
1.0

C

Draw blood into a container shown in
the left image, mix well, and separate
plasma.
Freeze the plasma samples forstorage.

Aldosterone,
CLEIA
plasma0.5 each

Containing
EDTA-2Na(2 or 5mL
vacuum blood
sampling tube)

Renin
concentration(ARC), CLEIA

Renin activity
Additives:
EDTA-2Na(PRA), EIA
3.0 or 7.5 mg
For 2 mL
(PN2)

Storageconditions:Roomtemperature
Shelf-life:
2 years

Aldosterone,
CLEIA/Renin
concentration
ratio

Aldosterone,
CLEIA/Renin
activity ratio

Cyclic AMP
For 5 mL
(PN5)

container handling method�After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Be sure to freeze the plasma sample
for storage.

whole
blood
1.5–2.0
each

Refrigerated

plasma0.7

plasma
1.0
whole
blood
4.0
each
Refrigerated

whole
blood
1.5–2.0
each

Refrigerated

F

After drawing blood into a container
shown in the left image, mix well, andseparate plasma at low temperature(4℃ ).
Be sure to freeze the plasma sample
for storage.
After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Be sure to freeze the plasma sample
for storage.

plasma1.2

After drawing blood into a container
shown in the left image, mix well, andseparate plasma at low temperature(4℃ ).
Be sure to freeze the plasma sample
for storage.

plasma0.3

After drawing blood into a container
shown in the left image, mix well, andseparate plasma at low temperature(4℃ ).
Be sure to freeze the plasma sample
for storage.

container handling method
CONTAINER FORM

PN2
PN5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Human brain
natriuretic
peptide (BNP)

Previous container symbol

Immediately

plasma0.5

FDrawing blood into a container shownin the left image in resting conditions,and mix well. Separate plasma within
6 hours in refrigerated conditions.
Promptly freeze the plasma for storage.

R

After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

F

After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Be sure to freeze the plasma sample
for storage.

C

PIVKA-II

Pro-gastrinreleasing peptide(ProGRP)
Bird allergenspecific IgG
antibody

whole
blood
1.5–2.0
each

plasma0.5

plasma0.4

SAMPLE HANDLING METHOD

After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

plasma0.5

ABO bloodgroups test
Rh (D) bloodgroup test

For 2 mL
(PN2)

Containing
EDTA-2Na(2 or 5mL
vacuum blood
sampling tube)

Blood group
Rh-Hr type

Blood type

Additives:incompatibility
EDTA-2Na
3.0 or 7.5 mg
Storageconditions:Roomtemperature
Shelf-life:
2 years

serum
whole blood Immediately
4.0
10.0–12.0
and(in aseparate
container)whole bloodandwhole blood (with EDTA-2Na)
2.0
2.0

Direct coombs
test

whole blood(with EDTA-2Na)
1.0

Irregular
antibody(Antibody
identification/
antibody titermeasurement)

serum
whole blood
10.0–15.0 Immediately 5.0
and
(in a
separate
container)andwhole blood
whole blood (with EDTA-2Na)
2.0
2.0

Pentraxin 3(PTX3)

Interleukin-8(IL-8)
Multiplemyelomamultiparameter
flow cytometry(8-color)

R

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.

Draw 2.0 mL of blood into a container
shown in the left image, mix well, andpromptly submit the specimen underroom temperature conditions.
Also, draw 10.0 to 12.0 mL of bloodin a separate container, promptly
separate serum, and refrigerate thespecimen.
Avoid putting in another request at
the same time.
R
After drawing blood into a container
shown in the left image, mix well, andpromptly submit the specimen in room
temperature conditions.
Avoid putting in another request at
the same time.
Draw 2.0 mL of blood into a container
shown in the left image, mix well, andRpromptly submit the specimen underroom temperature conditions.
Also, draw 10.0 to 15.0 mL of blood ina separate container, promptly
separate serum, and refrigerate theR specimen.
Avoid putting in another request at
the same time.

Promptly after drawing blood into
a container shown in the left image,
Immediately plasmamix well, and separate plasma at low
Refrigerated
0.3
temperature (4℃ ).
whole
Be sure to freeze the plasma sample
blood
F for storage.
1.5–2.0
After drawing blood into a container
eachshown in the left image, mix well, andplasmaseparate plasma.
0.5
Be sure to freeze the plasma sample
for storage.
Obtain 1.0 mL of bone marrow fluid
into a container shown in the left
bone marrow fluid
image (PN5 or PN2), mix well, andR(with EDTA-2Na)store at room temperature.
2.0
Please submit a sample on the day it
is collected.

�container handling method

For 5 mL
(PN5)

whole blood(with EDTA-2Na)
2.0 eachcontainer handling method
CONTAINER FORM

PN2
PN5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

FLT3/ITD
mutationanalysis

Previous container symbol

NPM1
mutational
analysisC

RET genemutationanalysis,Medullary
carcinoma of the
thyroid
Single siteanalysis for RET
Containing
EDTA-2Na(2 or 5mL
vacuum blood
sampling tube)
For 2 mL
(PN2)

Additives:
EDTA-2Na
3.0 or 7.5 mg
Storageconditions:Roomtemperature
Shelf-life:
2 years

TTR geneanalysis(Familialamyloidosis)
TACSTD2
genetic
analysis(cornea
dystrophy)
Spinocerebellar
degeneration
gene analysis
MEFV gene
analysis(Familial
Mediterranean
fever)
Y chromosome
microdeletion(AZF region)
Drug metabolizing
enzyme cytochrome
P450 CYP2C19 gene
polymorphism analysis

container handling method

For 5 mL(PN5)

UGT1A1 gene
polymorphism
analysis
NUDT15 gene codon
139 polymorphism
analysis�whole blood(with EDTA-2Na)
2.0 each

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
R
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

container handling method
CONTAINER FORM

PN2
PN5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

HLA-A, B,serological typing

Previous container symbolC

HLA-A,
DNA typing

Containing
EDTA-2Na(2 or 5mL
vacuum blood
sampling tube)
Additives:
EDTA-2Na
3.0 or 7.5 mg
For 2 mL
(PN2)

Storageconditions:Roomtemperature
Shelf-life:
2 years

HLA-B,
DNA typing

HLA-C,
DNA typing
whole blood(with EDTA-2Na)
2.0 each

HLA-DR,
serological typing

HLA-DRB1,
DNA typing

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
When requesting at least 2 HLA test
items, the sufficient amount for blood
test is 2.0 mL.
Still, recipients may have a decreased
number of cells after chemotherapy
or other treatments, which may makeit difficult to recover DNA; therefore,
R make sure to submit at least 2×106
cells. Recipients with WBC countbelow 1000/μL need to provide atleast two 2.0mL sampling tubes(Container PN2) or one 5.0mL
sampling tube (Container PN5).
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

HLA-DPB1,
DNA typing

For 5 mL
(PN5)

HLA-DQB1,
DNA typing

container handling method�container handling method
CONTAINER FORM

PN5

TEST NAME
Herpes Simplex
virus DNA,
qualitative

Previous container symbol

C

Varicella-zoster
virus DNA,
qualitative

Cytomegalovirus
pp65 antigen(C7-HRP)AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

whole blood(with EDTA-2Na)
2.0 eachwhole blood(with EDTA-2Na)
3.0

Containing
EDTA-2Na
Cytomegalovirus(5mL vacuum
DNA, qualitative
blood sampling
tube)
whole blood(with EDTA-2Na)
2.0 each

Additives:
EDTA-2Na
7.5 mgStorageconditions:Roomtemperature
Shelf-life:
2 years

Epstein-Barrvirus DNA,quantitative
whole
blood
(with
EDTA2Na)
5.0

container handling method

Humanherpesvirus,
type 6 DNA,
qualitative
Humanherpesvirus,
type 7 DNA,
qualitative�

plasma2.0

whole blood(with EDTA-2Na)
2.0 each

SAMPLE HANDLING METHOD

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
R
In this method, the result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.
After drawing blood into a container
shown in the left image, mix well, andstore at room temperature. Submit
the specimens promptly after bloodRsampling.
If blood within 24 hours aftercollection is not used, the detection
rate will decrease.
After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
In this method, the result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.
After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.
R After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.
After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
In this method, the result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.

container handling method
CONTAINER FORM

PN5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

High sensitivity
PNH testing

Previous container symbolC

JAK2V617F
mutational
analysis
MPN genemutational
analysis
SNRPN gene
analysis,
MethylationSpecific PCR
Containing
Prader-Willi
EDTA-2Na
syndrome(5mL vacuum
blood sampling Angelman
syndrome
tube)
Congenital long
Additives:
QT syndrome
EDTA-2Na
gene analysis
7.5 mgStorageconditions:Roomtemperature
Shelf-life:
2 years

HTT gene CAG
repeat sequence

whole blood(with EDTA-2Na)
5.0 eachR

SAMPLE HANDLING METHOD
Be sure to collect the specimen into a
container shown in the left image, andsubmit it in refrigerated conditions.
Avoid putting in another request at
the same time.

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.

Androgen receptor gene
CAG repeat sequence
IL28B SNPs
analysis

HLA DNA typing,
NGS-SBT method

After drawing blood into a container
shown in the left image, mix well,and refrigerate the specimen. Still,recipients may have a decreased
number of cells after chemotherapy
or other treatments, which may makeit difficult to recover DNA; therefore,
recipients with WBC count below
1000/μL need to provide at least two
5.0 mL sampling tubes of blood (PN5
container). In this method, a result isaffected proportionally to the degreeof contamination. Therefore, pay
careful attention when collecting andhandling samples.

container handling method�container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

AIRS for males(with cognitive
assessments)

PN5
Previous container symbol

C

AIRS for females(with cognitive
assessments)
AIRS for males(without cognitive
assessments)
AIRS for females(without cognitivewholeassessments)

Immediately

blood Refrigerated
5.0 eachplasma0.5
each

AICS for males(5 types)
Containing
EDTA-2Na(5mL vacuum
blood sampling
AICS for females
tube)(6 types)

Additives:
EDTA-2Na
7.5 mgStorageconditions:RoomtemperatureF

SAMPLE HANDLING METHOD
1. Collect approximately 5mL of bloodin an EDTA-2Na-containing tube
(left image).
2. Immediately after collecting, gently
mix the blood by inverting the tube
2 to 3 times (do not use a roller for
mixing).
3. Immediately after mixing (within 1
minute after mixing), cool the tubeby immersing in ice water (so thatthe tube is immersed in an ice bath
up to the level of the surface ofblood) (≥15 minutes, until ready forcentrifuge).
4. Within 8 hours of blood sampling,
centrifuge the sample underrefrigerated conditions (4℃ , 3000
rpm, 15 minutes) or under normal
conditions (3000 rpm, 15 minutes,
no temperature rise of the rotor).
5. Immediately after thecentrifugation, collect thesupernatant plasma from the centerof it without touching the interface
with the blood, and dispense it.
6. Within 4 hours of dispensing, freeze
the plasma samples for storage.

LHON mt DNA
Evaluation

Shelf-life:
2 years

MELAS mt DNA
Evaluation
whole blood（with EDTA-2Na）
8.0×2

MERRF mt DNA
Evaluation

container handling method

NARP mt DNA
Evaluation�

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
R
In this method, the result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.

container handling method
CONTAINER FORM

PN7

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

Antidiuretic
hormone (AVP)

Previous container symbol

A

Catecholamine
3 fractionation
A: adrenaline
NA: noradrenaline
DA: dopamine

Immediately
Refrigerated

plasma1.5
eachF

Promptly after drawing blood into
a container shown in the left image,mix well, and separate plasma at low
temperature (4℃ ).
Be sure to freeze the plasma sample
for storage.

R

After drawing blood into a container
shown in the left image, mix well, andseparate plasma.
Refrigerate the plasma samples.

L-dopaDopamine, total
HVA
VMA

Serotonin

5-Hydroxyindoleacetic

Acid (5-HIAA)
Containing
EDTA-2Na(7mL vacuum
blood sampling 3-Methoxy-4tube)
Hydroxyphenylglycol
Additives:
EDTA-2Na
10.5 mg
Storageconditions:Roomtemperature(MHPG)

Herpes simplex
virus DNA,quantitative

Shelf-life:
2 years

Varicella-zoster
virus DNA,quantitative

Epstein-Barrvirus DNA
(Clonality)
Humanherpesvirus,
type 6 DNA
quantitative

Promptly after drawing blood into
a container shown in the left image,mix well, and separate plasma at low
Immediately plasma
temperature (4℃ ) at 900 rpm for 20
F
Refrigerated
1.5
minutes (PRP) or at 1500 rpm for 10
minutes (P).
Be sure to freeze the plasma forstorage.
After drawing blood into a container
shown in the left image, mix well, andR
separate plasma.
Refrigerate the plasma samples.
plasmaAfter drawing blood into a container
1.5 eachshown in the left image, mix well, andF separate plasma.
Be sure to freeze the plasma sample
for storage.
After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting and handlingsamples.
After drawing blood into a container
shown in the left image, mix well, andwhole blood
refrigerate the specimen.
(with EDTA-2Na)In this method, a result is affected
5.0 each
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.
After drawing blood into a containerRshown in the left image, mix well,and refrigerate the specimen. Submit
the specimens promptly after blood
sampling.
If blood within 24 hours aftercollection is not used, the detection
rate will decrease.
whole blood(with EDTA-2Na)
7.0

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.

whole blood(with EDTA-2Na)
5.0

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

�container handling method

Cytomegalovirus
pp65 antigen(C10, C11)

whole
blood
4.0–5.0
each

plasma1.5 eachcontainer handling method
CONTAINER FORM

PN7

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

HTLV-1 nucleic
acid detection
qualitative

Previous container symbol

A

SAMPLE HANDLING METHODHTLV-1
Provirus DNA
qualitative

After drawing blood into a container
shown in the left image, mix well, andsubmit the specimen in refrigerated
conditions.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

HTLV-I (ATLV)
Provirus DNA(Clonality)

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.
Avoid putting in another request at
the same time.

MicroArray
chromosomal test
(Chromosomal
structuralvariation analysis)
KIT sequence
analysis (leukemia)
Screening ofchimeric genes
related to leukemia,
quantitative

Containing
EDTA-2Na(7mL vacuum
blood sampling WT1 mRNA
quantitativetube)
Additives:
EDTA-2Na
10.5 mg
Storageconditions:Roomtemperature
Shelf-life:
2 years

Major BCR-ABL1
mRNA (IS)
Major BCR-ABL1mRNA(％)
Major BCR-ABL1
mRNA, qualitative
Mutation analysis in the ABL1
region, Major BCR-ABL1
minor BCR-ABL1mRNA(％)
minor BCR-ABL1
mRNA,quantitative

container handling methodminor BCR-ABL1
mRNA, qualitative
micro BCR-ABL1
mRNA, qualitative
Mutation analysis in the ABL1
region, minor BCR-ABL1
TCF3-PBX1 mRNA,
quantitative
TCF3-PBX1 mRNA,
qualitative
PML-RARA mRNA,
quantitative
PML-RARA mRNA,
qualitative�

whole blood(with EDTA-2Na)
7.0 eachR

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

container handling method
CONTAINER FORM

PN7
Previous container symbol

A

TEST NAMESAMPLE HANDLING METHOD

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
Please submit a sample on the day it
is collected.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

whole blood(with EDTA-2Na)
7.0 eachR

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.

�container handling method

CBFB-MYH11mRNA, quantitative
CBFB-MYH11mRNA, qualitative
RUNX1-RUNX1T1
mRNA, quantitative
RUNX1-RUNX1T1
mRNA, qualitative
RUNX1-MECOM
mRNA, qualitative
ETV6-RUNX1
mRNA, quantitative
ETV6-RUNX1
mRNA, qualitative
KMT2A-AFF1
mRNA, quantitative
KMT2A-AFF1
mRNA, qualitative
KMT2A-AFDN
mRNA, quantitative
KMT2A-AFDN
mRNA, qualitative
KMT2A-MLLT3
Containing
mRNA, qualitative
EDTA-2Na(7mL vacuum
KMT2A-MLLT3
blood sampling mRNA, quantitativetube)
KMT2A-MLLT1
mRNA, quantitative
Additives:
EDTA-2Na
KMT2A-MLLT1
10.5 mg
mRNA, qualitative
Storage
NUP98-HOXA9
conditions:mRNA, quantitative
Room
temperature STIL-TAL1 mRNA,
quantitative
Shelf-life:
DEK-NUP214
2 years
mRNA, quantitative
DEK-NUP214
mRNA, qualitative
T-cell receptor β-chain
Cβ1 rearrangement
T-cell receptor β-chain
Jβ1 rearrangement
T-cell receptor β-chain
Jβ2 rearrangement
T-cell receptor γ-chainJγ rearrangement
T-cell receptor δ-chainJδ1 rearrangement
Immunoglobulin H-chain
JH rearrangement
Immunoglobulin H-chain
Cμ rearrangement
Immunoglobulin L-chainJκ rearrangement
Immunoglobulin L-chainCκ rearrangement
Immunoglobulin L-chain
Cλ rearrangement

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHODPRRT2 genemutational
analysis

PN7
Previous container symbol

A

MECP2 (exons 3
and 4) mutation
analysis

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

Dystrophin DNA
Containing
EDTA-2Na(7mL vacuum
blood samplingtube)
Additives:
EDTA-2Na
10.5 mg
Storageconditions:Roomtemperature
Shelf-life:
2 years

Fukuyama-typecongenital
musculardystrophy DNA
insertion
Chimerism
analysis,
pre-transplant,
recipient, PCR

whole blood(with EDTA-2Na)
7.0 eachR

Chimerism
analysis,
pre-transplant,
donor, PCR

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

Chimerism
analysis,post-transplant,
PCR

CONTAINER FORMTEST NAME

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen. Also, makesure to submit a pair of specimens
taken from a recipient and from adonor.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention whencollecting and handling samples.

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

Containing thepreservation
Previous container symbol solution(5mL
vacuum bloodRsampling tube)

PNK

Additives:
Preservation
solution 0.7 mL

Natural killer

container handling method

Storagecell activity
conditions:
Refrigeration
Shelf-life:
1 year(1 monthafter opening
the aluminum
sheet)

�whole blood(with thepreservation
solution)
5.0

After drawing blood into a container
shown in the left image, mix well, andrefrigerate the specimen.
R Draw a larger amount of blood frompatients with low levels of
lymphocytes. Please submit a sample
on the day it is collected.

container handling method
CONTAINER FORM

PNM
Previous container symbol

g2

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

BRCA1/2 genetest (Breast
cancer)Containing
BRCA1/2 gene
EDTA-2K (10mL
test (Ovarianvacuum blood
sampling tube) cancer)
BRCA1/2 gene

Additives:
test (HBOC)EDTA-2K 18mg
Storageconditions:Roomtemperature
Shelf-life:
1 year

BRCA1/2 genetest (Pancreaticcancer)
BRCA1/2 genetest (Prostatecancer)Single site
testing for BRCA
1/2 gene

whole blood(with EDTA-2K)
7.0 each

SAMPLE HANDLING METHOD

After drawing blood into a container
shown in the left image, mix well, andstore at room temperature.
Avoid putting in another request at
the same time.
In this method, a result is affectedR
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.
Please submit a sample on the day it
is collected.

container handling method�container handling method
CONTAINER FORM

PSF
Previous container symbol

P3

CONTAINER FORM

S09
S06

Containing
separating
agent(9 or 6mL
vacuum blood
sampling tube)
Storageconditions:Roomtemperature
Shelf-life:
1 yearcontainer handling method

For 6 mL(S06)

�AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Containing
EDTA-2K +Cytomegalovirus
plasmanucleic acidseparating
quantitativeagent(5mL vacuum
blood sampling
whole
tube)
blood
5.0 each
Storageconditions:4-25℃
HIV-1 RNA
Shelf-life:
1 yearFor 9 mL
(S09)

TEST NAME

plasma1.8 eachFquantitative

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED
(mL)
(mL)

SAMPLE HANDLING METHOD

Draw the specified amount of blood (8mL) into a container shown in the left
image, gently invert the tube 4 to 5
times, and centrifuge the tube at room
temperature. Freeze the specimen in
the tube and submit it under frozen
conditions. Avoid putting in anotherrequest at the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

SAMPLE HANDLING METHOD

After drawing the necessary amountof blood into a container shown in theleft image depending on the test,
gently invert the tube 4 to 5 times,
and allow to stand at room
temperature for 30 to 60 minutes.
After coagulation, centrifuge thesample. Transfer the serum into thecontainer A00 (polyethylene testtube), and submit it.
Please make sure that a sufficientamount of specimen is provided.

Generalbiochemical
As
As
tests
The centrifugation conditions should
appropriate appropriate be 2000G for 10 minutes.
Generalserological tests(The table below shows the rotor
othersradius and the speed [rpm] at 2000G.)
Radius
(cm)
10
12
14
16
18
20

Conversion table
Speed
Radius
(rpm)
(cm)
4200
22
3800
24
3500
26
3300
28
3100
30
3000

Speed
(rpm)
2800
2700
2600
2500
2400

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

S3F
S5F
Previous container symbolP1

HBV DNA
quantitative, IU

Containing
coagulation
accelerator +serumseparating
agent(3 or 5mL
vacuum blood
For collecting sampling tube)
3 mL of blood
Storage(S3F)
conditions:Roomtemperature

whole
blood
5.0

serum
1.8

HCV RNA
core genotype

whole
blood
3.0

serum
0.5

HCV RNA
quantitative

whole
blood
5.0

serum
1.8

Shelf-life:
1 yearF

Draw the specified amount of blood
into a container shown in the left
image, gently invert the tube 4 to 5
times, and allow to stand at room
temperature for 30 to 60 minutes.
After coagulation, centrifuge the tube.
Freeze the specimen in the tube andsubmit it under frozen conditions.
Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

For collecting
5mL of blood(S5F)

container handling method�container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED
(mL)
(mL)

SAMPLE HANDLING METHOD

S9P
S7P

For 10mL(S9P)

Not
containing
separating
agent(10 or 7mL
vacuum blood
sampling tube)
Storageconditions:Roomtemperature
Shelf-life:
1 yearFor 7mL(S7P)

container handling method�After drawing the necessary amountof blood into a container shown in theleft image depending on the test,
gently invert the tube 4 to 5 times,
and allow to stand at room
temperature for 30 to 60 minutes.
After coagulation, centrifuge thesample. Transfer the serum into thecontainer A00 (polyethylene testtube), and submit it.

drug testothers

The centrifugation conditions shouldAsAs
be 2000G for 10minutes.
appropriate appropriate (The table below shows the rotor
radius and the speed [rpm] at 2000G.)
Radius
(cm)
10
12
14
16
18
20

Conversion table
Speed
Radius
(rpm)
(cm)
4200
22
3800
24
3500
26
3300
28
3100
30
3000

Speed
(rpm)
2800
2700
2600
2500
2400

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHODSZZContaining
serum-separating
Previous container symbol
agent andecoagulation
accelerator(3mL vacuum blood
sampling tube)
Additives:
Thrombin
Heparinneutralizer

Aluminum (Al)

whole
blood
2.5–3.0

serum
0.6

Storage conditions:Roomtemperature

To prevent contamination, collectblood from a shunt by instillation or
using a vacuum tube.
Immediately after blood sampling,Rthoroughly mix the blood by inverting
the tube. After centrifugation, submit
the specimen under refrigerated
conditions.

Shelf-life:
1 year

CONTAINER FORM

U00

Storage conditions: Room temperature

Previous container symbol

Y

Urine container

container handling method�container handling method
CONTAINER FORMTEST NAME

U10

Chlamydia trachomatis DNA
Neisseria gonorrhoeae DNA
Simultaneous identification of DNA of N. gonorrhoeae and C. trachomatis
Detection of Trichomonas vaginalis (TV) and Mycoplasma genitalium (MG) DNA

Previous container symbol

Y1

STORE
TEMPERATURE

R

Sterile dropping ●Obtaining specimens from urine
pipetteCollect the first-voided urine at least 1 hour after the last urination.
Sterile cup(1) Collect the first-voided urine in a sterile cup, and transfer the urine into the dedicatedcontainer using a sterile dropping pipette.
(2) Fill the urine is to the level between the lines on the dedicated container.
(3) Firmly tighten the cap of the dedicated container.
(4) Invert the dedicated container 5 times, and submit the specimen under room temperature.

2

3

4

rea

1

Fill A

Invert 5
times.

●Obtaining from mouth wash specimens
Patient must refrain from eating, gargling, brushing teeth, or chewing gum before collecting
specimens.
(1) Take 15 to 20 mL of sterile physiological saline in the mouth, tilt the head backward, andgargle vigorously for 10 to 20 seconds.
(2) Collect all of the mouth wash specimen in a cup, and transfer it to the dedicated containerusing a dropping pipette to the level between the lines on the container.
(3) Firmly tighten the cap of the dedicated container, invert the container for 5 times, andsubmit the specimen under room temperature.
※However, “Detection of Trichomonas vaginalis (TV) and Mycoplasma genitalium (MG) DNA”
cannot be requested.

2

Storageconditions:
Room temperature

3

rea

1

Fill A

Additives: Guanidinehydrochloride

Shelf-life: 1 year

CONTAINER FORM

U20
Previous container symbol

Urine container
Storage conditions: Room temperature

container handling method

Y0

CONTAINER FORM

U40
Previous container symbol

Y4

Pregnanediol/Pregnanetriol�Invert 5
times.

For Urinalysis, Urinary sediment
Storage conditions: Room temperaturecontainer handling method
CONTAINER FORM

U50
Previous container symbol

Y5

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Containing
preservative(Container
capability of
10mL)
Storageconditions:Roomtemperature

Myoglobin,
Urine, CLEIA

random urine
6

SAMPLE HANDLING METHOD

Promptly after sampling, pourcollected urine into the dedicatedcontainer (U50) up to the printed line
R on the label, mix well, and submit the
specimen under refrigerated
conditions.
Avoid freezing.

Shelf-life:
2 years

CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Containing Tris +HclPrevious container symbol (Container
capability of 10mL)

SAMPLE HANDLING METHOD

U70
Y7

Additives:
1.5 M Tris-Hcl
0.5 mL

Collagen type IV,
Urine

random urine
5

Storage conditions:
Room temperature

Collect the first-void urine in the earlymorning, and transfer into a container
shown in the left image. After mixingRwell, refrigerate the specimen.
Avoid freezing and putting in anotherrequest at the same time.

Shelf-life:
2 years

CONTAINER FORMTEST NAME

Containing thepreservation
Previous container symbol solution(Container
Y8capability of 10 mL)

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

U80

randomAdditives:
Urine stabilizer

urine
Nuclear matrix
random urineAs
protein 22, Urine appropriate Immediately (supernatant)5
Storage conditions: (NMP22)(Separate
Protection fromlight at room
temperature

container)

Promptly after urine sampling,
centrifuge the urine. Transfer thesupernatant into the dedicatedcontainer to the specimen sampling
R level, and mix well.
Avoid freezing and putting in anotherrequest at the same time. Submit the
specimen under refrigerated
conditions.

CONTAINER FORM

U90
Previous container symbolb2

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

Containing theantiplasmin agent
Additives:
Aprotinin
Purified gelatin
Dehydroacetic acid
SodiumStorage conditions:Roomtemperature

FDP,
quantitative

randomurine random urine(fresh (supernatant)urine)
0.5
2

F

Collect fresh urine, and transfer into
a container shown in the left image.
After mixing well, centrifuge at 3000
rpm for 5 to 10 minutes.
Be sure to freeze the supernatant(random urine).

Shelf-life:
2 years(1 week after
opening thealuminum package)�

container handling method

Shelf-life:
2 years and6 months

container handling method
CONTAINER FORMTEST NAMESTORETEMPERATURESAMPLING METHODUV6Previous container symbol

Centrifuge the appropriate
amount of urine collected at
1500 rpm for 5 minutes.

f3

Urine
cytodiagnosis(LBC)

Remove the supernatantand mix the sediment well.
R

Pipette the sediment andtransfer it into a dedicatedcontainer. Firmly tighten
the cap before submission.
(If the amount of thesediment is too small, put
the solution in the dedicatedcontainer into a test tube
and transfer it again into
the container.)

Additives:
Methanol 55%
Storage conditions:
Room temperature
Shelf-life:
1 year and 6 months

CONTAINER FORMTEST NAMESTORETEMPERATUREUV7SAMPLING METHOD
Centrifuge the properamount of collected urine(≥33mL) at 1500rpm for 5
minutes.

Previous container symbol

f7Remove the supernatantand mix the sediment well.

Bladder cancer
FISH (UroVysion)container handling method

Additives:
Methanol 55%
Storage conditions:
Room temperature
Shelf-life:
1 year and 6 months

�R

Pipette the sediment andtransfer it into a dedicatedcontainer before freezing.
Confirm that the lid of thecontainer is tightly closed
before submission without
wrapping with a parafilm.
●Points to note
Pay attention to discarding the supernatant after
centrifuge because if the cell count in the submitted
container is insufficient, the test may be impossible.

container handling method
CONTAINER FORMTEST NAMESTORETEMPERATURE

V10

SAMPLE HANDLING METHOD●S wab of affected area, vesicle fluid, stool, etc.
Wipe affected area with a sterile cotton swab. Transfer the
specimen into a container with virus preservation solution
according to the methods shown in the figures below. Then,
refrigerate the specimen.

Previous container symbolV

Rub the swab headagainst the interiorwall of the container.

Withdraw
the cotton
swab.

Firmly tighten the capof the container, andsubmit the specimen underrefrigerated conditions.

Viruses isolation

Preserration solutiom for viruses
Stool

Stick the swab into thestool several times.

Roll the swab overthe surface of thestool.

Dedicated container for virusesContaining the preservation
solutionR

Storage conditions:
Refrigeration
Shelf-life: 6 months●U rine, cerebrospinal fluid, pleural fluid, nasal discharge, etc.
Collect a specimen of an equivalent volume to the virus
preservation solution according to the method shown in figures
below, and refrigerate the specimen.

Viruses
identification

Firmly tighten the cap of
the container, and submit
the specimen underrefrigerated conditions.

2.5mL
2.5mL

●T
 issues
Collect a small piece of tissue specimen (about 5 mm cube), andplace it into the virus preservation solution. Then, refrigerate
the specimen.

�container handling method

A liquid specimen (e.g., urine)
should be mixed with anequivalent volume of virus
preservation solution.
Even if a specimen volume
is lower than that of thepreservation solution, do notdiscard the preservation solution,and transfer as much specimen
as possible to the container.

container handling method
CONTAINER FORMTEST NAMESTORETEMPERATURE

V30

Herpes simplex virus specific antigen
Varicella-zoster virus antigen

F

Previous container symbol

W1

Storageconditions:
Room temperature
Shelf-life:
1 yearcontainer handling method

�●SAMPLING METHOD
Conduct sampling which enables to collect abundant focal basal cells.
Early-stage vesicle is optimal as specimen. Vesicle ﬂuid and pus are inappropriate as specimen.
(1) Peel upper surface or crust with a sterile needle. (Figure 1)(2) Remove upper surface which covers the focal site with forceps etc. (Figure 2)(3) Moisten the cotton swab with physiological saline.
(4) Wipe vigorously the entire basal area since cells infected with viruses exist in this area. (Figure 3)
Note
Wipe out pus with a cotton swab if any, and collect specimen with other cotton swab. While
applying this procedure, make sure not to make a mess of basal cells.
●Procedures
Sample smearing(1) Smear sample on the two circles of the glass slide, rotating a cotton swab. While this procedure, place the cotton swab
paralleled to the glass slide to smear the entire area of the swab. Pay attention not to smear disproportionately. (Figure 4)(2) Make sure to check the uniform distribution of the specimen before discarding a cotton swab. Uniform distribution
provides opaque view. If there is any transparent area, smear on this area again with a cotton swab placed parallel.
(3) Then apply air dry.
(4) Add suﬃcient amount of acetone (enable to be distributed to the entire specimen) on the dried glass slide, then make it evaporated.
・Submit two glass slides containing specimen.
Sample storing
The completely dried glass slides should be kept in an object case with the smeared surface
down and cryopreserved after writing down the test name, hospital name, and subject name.
Figure 1

Figure 2

Figure 3

Figure 4
Basal cellscontainer handling method
CONTAINER FORM

V41
Previous container symbol

f6

TEST NAMESAMPLING METHODSTORETEMPERATURE

1

Human
papillomavirus
DNA (genotypes
16, 18, and otherhigh-risk groups)(1) Collect cervical cells
Collect cells using an
instrument other thancotton swab (e.g., brush,
spatula).

2

Humanpapillomavirus
DNA (high-risk
group) (LBC)3

Humanpapillomavirus(HPV)genotyping

Additives:
Methanol 55%
Storage conditions:
Room temperature
Shelf-life:
1 year and 6 months

Cytodiagnosis(Gynecologic LBC,
Bethesda system)

Cytodiagnosis(Gynecologic LBC)

R

(2) Release cells
Place the sampling device into thecontainer, and rinse the sampling device
by pushing the broom into the bottom of
the container 10 times, forcing the bristles
apart. Then, swirl the broom vigorously tofurther release the collected cells.
(3) Tighten the cap
Remove the sampling device, andtighten the cap so that pass the blackline the container to the left side. Storethe specimen at room temperature.
(Note) D o not leave any part of thesampling device in the container.

[Note]
When collecting cells from pregnant women, use
cotton swab instead of brush or spatula inconsideration of the safety. To collect thenecessary amount of cells for the test with cottonswabs, remove mucus with a cotton swab inadvance, and use another cotton swab for collecting
cells. After collection, thoroughly rinse the cottonswab used for the collection in the preservation
solution to release the cells. Do not leave any partof the cotton swab in the container. Please notethat the swab shaft may be broken if an excessive
force is applied to the shaft.

container handling method�container handling method
CONTAINER FORM

V50
Previous container symbol

F3

TEST NAMESAMPLING METHODSTORETEMPERATURE

1

Chlamydia
trachomatis
DNA
2

Insert the attached FLOQSwabs into thecervical canal. Gently rotate the swab
towards the same direction for 5 rounds, andwithdraw the swab carefully while avoiding
any contact with the vaginal mucus.

3

Open the cap of the dedicated container, andcarefully place the FLOQSwabs into the container
while avoiding immersing the tip of the swab in
the container solution. Align the score line of theswab shaft with the top edge of the container.

Neisseriagonorrhoeae
DNA
Simultaneous
identification of
DNA of N.
gonorrhoeae
and C.
trachomatis
Detection of
Trichomonas
vaginalis (TV)
Additives: Guanidine hydrochloride and Mycoplasma
Storage conditions: Room temperature genitalium (MG)DNA
Shelf-life: 1 year

container handling method�Remove excess mucus from the cervicalcanal and the surroundings using the dryswab attached in the swab sampling kit.
Discard this swab.

R

4

Break the FLOQSwabs shaft using the edge ofthe dedicated container, and discard the top
portion of the swab shaft. Firmly tighten thecap of the dedicated container, and submit
the specimen under room temperature.

container handling method
CONTAINER FORM

V60
Previous container symbol

W3

Additives: Preservation solution
Storage conditions: Room temperature
Shelf-life: 3 years

For general use

For pregnant women

Human papillomavirus DNA (high-risk group, low-risk group)[Note]Please note that the swab shaft may be broken if an excessive force is applied to the shaft while collecting sample.

container handling method�container handling method

CONTAINER FORM

VP0

CONTAINER FORM

VP1

Pathology container

Previous container symbol

Preparation kit for frozen tissue specimens

Previous container symbolu5

u1Fill the container with 20% formalin solution.

Storage conditions: Room temperature

Storage conditions: Room temperature

Shelf-life: 1 year

CONTAINER FORMTEST NAMESAMPLING METHODSTORETEMPERATURE

Insert a dedicated cotton swab into theposterior vaginal vault, and rotate the swab
about 10 seconds to allow it to absorb
secretions.
* Do not rub the vaginal surface vigorously.
* Avoid any mucus from being contained into thespecimen.

VP5
Previous container symbol

W5

Remove the purple cap of the specimen
extraction container. Immerse the secretioncontaining swab in the extraction buffer, andswirl the swab 5 times. (Do not spill thecontents of the container.)Take out the cotton swab from the specimen
extraction container.

Human
oncofetal
fibronectinF

container handling method

Attach a specimen filter to the specimen
extraction container.

Additives:
Extraction
bufferStorageconditions:Roomtemperature
Shelf-life:
1 year�Specimen
for assay

Drop all of the specimen extract into thespecimen storage tube. Close the cap of thetube, and be sure to freeze it.

● Points to note
• Collect specimen before washing the vagina.
• If sperm are contained in a specimen, do not use it.
• If ≥0.1% of blood is contained in a specimen, accurate results may not be
obtained.
• Collect specimen from the posterior vaginal vault.
• Avoid rubbing the vaginal surface vigorously.

container handling method
CONTAINER FORMTEST NAMESTORETEMPERATURE

VP6

Granulocyte elastase in cervical mucus

F

Previous container symbol

SAMPLING METHOD

W6

Gently remove excess mucus fromthe vaginal area using a swab.

Correct site forcollecting specimen

Insert the specimen collection swab
into the endocervical canal. Note 3)●Points to note
1) Collect specimen from the endocervical canal.
2) Collect specimen before washing the vagina.
3) Insert the swab while avoiding contact
with anything, such as secretion or mucus around theexternal os, till it reaches the endocervical canal.
4) Each rotation should take about 5 seconds toallow the swab to absorb mucus. If a small amountof solid matters are attached to the swab,
remove them with tweezers.
<Before sampling> <After sampling>

Mucus

Gently rotate the swab twice
toobtain cervical mucus. Note 4)

Solid matters5) Start extraction procedures of thecollected specimen within 15 minutes.
[Note]
Please note that the swab shaft may
be broken if an excessive force is applied
to the shaft while collecting specimen.

Extraction methods
Additives:
Extraction
buffer(Phosphatebuffer)Storageconditions:Roomtemperature(Refrigeration
for theextraction
buffer)Shelf-life: 1 year

(1)

(4)

Squeeze the cotton swab
by each container with
fingers to release thespecimen extract andremove the swab.

Unscrew the blue cap of
the extraction container
with the extraction
buffer.

(2)

Place the cotton swab with (3)the collected cervical mucus
in the container, and allow
to stand for 2 to 3 minutes.
Then, shake the swab in an
up-and-down direction for
20 to 30 times to extract the
specimen.

Set the filter.

(5)

Filter the specimen extract into the specimen storage container.
Apply only one pressure to the extraction container to obtain
5 to 7 drops (about 300 μL) of the extract. If the required amountof extract is not obtained,start the procedure all over again from
specimen collection. Seal the specimen storage container with the white
cap, and store it. Specimens should be tested within 3 days when
refrigerated (8 C) and within 3 months when frozen (≤‒15 C).

CONTAINER FORMTEST NAMESTORETEMPERATURE

VS4

Bordetella pertussis DNA

F

Previous container symbol

k9

SAMPLING METHOD

2. Hold the patient’s head so as to stay still, and gently insert the swab
into the postnasal space to collect mucus.

3. W ithdraw the swab, and promptly insert the swab into the sterile
polyethylene test tube (ARR). Align the slit score line of the swab
shaft with the top edge of the container, and break the shaft.
Storageconditions:
Room temperature

4. C lose the cap firmly while the swab is included in the container.
Submit the specimen under frozen conditions.

Shelf-life: 5 years�

container handling method

1. Take out a swab by holding the shaft. Do not touch the cotton part.

container handling method
CONTAINER FORM

X00

TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Nucleic acid
identification of
M. tuberculosis
complex, TRC

Previous container symbolℓSterile sputum
collection
container
Storageconditions:Roomtemperature

Nucleic acid
identification ofMAC, Real-time
TRC
Pneumocystis
carinii(P. jirovecii)
DNA

In this method, a result is affected
proportionally to the degree of
R contamination. Therefore, pay carefulattention when collecting andhandling samples.
sputum2.0 each

Mycoplasma
pneumoniae
DNA
Legionella DNA,
qualitative

SAMPLE HANDLING METHOD

F

sputum1.0

Avoid putting in another request at
the same time.
In this method, a result is affected
proportionally to the degree ofcontamination. Therefore, pay carefulattention when collecting andhandling samples.

CONTAINER FORM

X10
Previous container symbolf

Cytodiagnosis, sputum cell concentrations
Additives:
Saccomanno’s solution,
mucolytic agent
Storage conditions:
Room temperature (cold and dark place)
Shelf-life: 1 year

CONTAINER FORM

X90

Nucleic acid container「GM container」
Previous container symbol Contents Nucleic acid protector

L7

Storage conditions:
Room temperature
Shelf-life:
1 year from manufacturing datecontainer handling method

Lung cancer compact panel CDx / Lung cancer compact panel for research�container handling method
CONTAINER FORM

XC0

Storage conditions: Room temperature

Previous container symbol

L2

Dedicated container for calculi

CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

XR4

Collect sample in a container shown in
the left image, and freeze immediately.
Place the specimen into the containerset (Z50), and be sure to store frozen.

Previous container symbol

L4

Tau protein

CSF 1.0

Storageconditions:Roomtemperature
Shelf-life:
4 years

CONTAINER FORM

XR5

F

Cochlintomoprotein(CTP)

TEST NAME

auris media wash
solution 0.3

Collect sample in a container shown in
the left image, and be sure to freeze.

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

SAMPLE HANDLING METHOD

β-Amyloid
1-42/1-40 ratio

Previous container symbol

L6

SAMPLE HANDLING METHOD

CSF container
Storageconditions:Roomtemperature

Phospholylated
tau protein

CSF 2.5 eachR

Obtain CSF into a container shown in
the left image and please be sure torefrigerate the specimen.

container handling method

Shelf-life:
4 years

Tau protein,total (AD)�container handling method
CONTAINER FORM

Z10TEST NAME
Nasal
eosinophils

Previous container symbolt

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

nasal discharge
smear 2–3 slides

Prepare unstained slide, and write
section thickness on the front side of theslide. Place the slides into an object caseshown in the left image, and submit underroom temperature. When ordering any of
the three tests in the left (regardless ofthe number of tests ordered at the sametime), make sure to also place an orderof“Tumor site confirmation test(code No.
A910 1)”as well.

BRAF exon15
V600E (SEQ)

unstained slides• For tumor site
confirmation
PIK3CA mutation Section thickness 3.4
μm: 2 slides eachanalysis, SEQ• For DNA
extraction
c-kit mutation
Section thickness
analysis (GIST) 10 μm: 5–10 slides each

Generalwet-fixed
cytodiagnosis
2 smears(sputum)
Generalsmearscytodiagnosis(wet-fixed 1 slide,(other specimens dry-fixed 1 slide)than sputum)
Cytodiagnosis(Gynecology,
Bethesda system）
Cytodiagnosis(Gynecology)[Object cases]
Preparation(slide glass)
Storageconditions:Roomtemperature

Prepare stained smear slides. Placethe slides in an object case shown in
the left image, and submit under room
temperature.

wet-fixed smear
1 slide each

Stainedspecimen
preparation
CD30(IHC)

unstained
2 slides each

Malignantlymphoma ALK
protein, IHC

Breast cancer
HER2 protein,
IHC, 4B5

container handling method

unstained
4 slides each

Breast cancer
PD-L1 protein,
IHC, 22C3
Breast cancer
PD-L1 protein,
IHC, SP142�

SAMPLE HANDLING METHOD
Prepare smears with fresh nasal
discharge, and submit.
Store at room temperature.

R

Please thinly slice the tissue into 3
to 4 μm, use coated slides such assilanized slides, and apply it within 50
mm from the edge of the glass slide.

Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃
before submitting them. For drying
at high temperature, we recommend
processing within 30 minutes at
60℃ . Please avoid leaving it inhigh temperature for a long time.
Specimens that have undergone
decalcification cannot be requested
because correct results cannot be
obtained. Fixation time is within 6
to 72 hours in 10% neutral bufferedformalin (for biopsy specimens, it
depends on the size of the specimen.)is desirable. Thinly slice the tissue
into 4 μm and apply it onto the slide
at around the center.
Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃ before
submitting them. Slice the tissue tothe thickness of 4-5μm, and apply it
onto the slide at around the center.

container handling method
CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Z10
Previous container symboltGastric cancer
HER2 gene,
FISH

Gastric cancer
HER2 protein,
IHC

Gastric cancer
PD-L1 protein,
IHC, 28-8[Object cases]
Preparation(slide glass)
Storageconditions:Roomtemperature

Colorectal
cancer HER2
gene, FISH

Esophageal
cancer PD-L1
protein, IHC,
22C3

�container handling method

Colorectal
cancer HER2
protein, IHC

unstained
4 slides each

SAMPLE HANDLING METHOD

Use coated slides such as silanized slides, and leavethinly-sliced samples to dry overnight at about 40℃
before promptly submitting them. In the case of baking forthe purpose of preventing tissues from coming off, treat
them in as short a time as possible (less than 1 hour),and avoid leaving the glass slides at high temperatures
for any duration longer than that. Fixation time is 6 to 72
hours in 10% neutral buffered formalin (depending on thesize of specimens for biopsy samples with at least 6 hours
fixation for biopsy specimens. However, the fixation timeshould be at least 6 hours).Prepare thin slices of tissue
sections 4 to 6 μm in thickness.
Use coated slides such as silanized slides, and leave thinly-sliced
samples to dry overnight at about 40℃ before promptly submitting
them. In the case of baking for the purpose of preventing tissues
from coming off, treat them in as short a time as possible (less than
1 hour), and avoid leaving the glass slides at high temperatures forany duration longer than that. Fixation time is 6 to 72 hours in 10%
neutral buffered formalin (depending on the size of specimens forbiopsy samples with at least 6 hours fixation for biopsy specimens.
However, the fixation time should be at least 6 hours).Prepare thinslices of tissue sections 3 to 4 μm in thickness.
Use coated slides such as silanizedslides, and leave thinlysliced samples
to dry overnight at about 40℃ before
submitting them. Slice the tissue tothe thickness of 4-5μm, and apply it
onto the slide at around the center.
Use coated slides such as silanizedslides, and leave thinly-sliced
samples to dry overnight at about
40℃ before promptly submitting them.
In the case of baking for the purposeof preventing tissues from coming
off, treat them in as short a time
as possible (less than 1 hour), andR avoid leaving the glass slides at hightemperatures for any duration longer
than that. Fixation time is 6 to 72
hours in 10% neutral buffered formalin(depending on the size of specimens
for biopsy samples with at least 6
hours fixation for biopsy specimens.
However, the fixation time should be
at least 6 hours).
Prepare thin slices of tissue sections
4 to 6 μm in thickness.
Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃
before promptly submitting them. In
the case of baking for the purposeof preventing tissues from coming
off, treat them in as short a time
as possible (less than 1 hour), andavoid leaving the glass slides at hightemperatures for any duration longer
than that.
Fixation time is 6 to 72 hours in 10%
neutral buffered formalin (dependingon the size of specimens for biopsy
samples with at least 6 hours fixation
for biopsy specimens. However, thefixation time should be at least 6
hours).
Prepare thin slices of tissue sections
3 to 4 μm in thickness.
Use coated slides such as silanizedslides, and leave thinlysliced samples
to dry overnight at about 40℃ before
submitting them. Slice the tissue tothe thickness of 4-5μm, and apply it
onto the slide at around the center.

container handling method
CONTAINER FORM

Z10
Previous container symbolt[Object cases]
Preparation(slide glass)
Storageconditions:Roomtemperature

TEST NAME
Esophageal
cancer PD-L1
protein, IHC,
28-8
Uterine cervicalcancer PD-L1
protein, IHC,
22C3
MMR protein,
IHC
MMR protein,
IHC (Lynch
syndrome)
BRAF V600E
protein, IHC
Lung cancer, PDL1 protein, IHC,
22C3
Lung cancer, PDL1 protein, IHC,
28-8
Lung cancer, PDL1 protein, IHC,
SP142
Lung cancer PDL1 protein, IHC,
SP263
Lung cancer ALK
protein,Highly sensitive
IHC

AMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

unstained
4 slides each

unstained
9 slides eachcontainer handling method�

Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃ before
submitting them. Slice the tissue tothe thickness of 4-5μm, and apply it
onto the slide at around the center.

Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃ before
submitting them. Thinly slice thetissue into 4 μm and apply it onto theslide at around the center.
Use coated slides such as silanized slides,
and leave thinly-sliced samples to dry
overnight at about 40℃ before submitting
them. Thinly slice the tissue into 4 to 5 μm
and apply it onto the slide at around the
R center, ≥ 15 mm from the frosted edge and≥ 15 mm from the slide glass edge.

unstained
4 slides each

Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃ before
submitting them. Slice the tissue tothe thickness of 4-5μm, and apply it
onto the slide at around the center.
Use poly-l-lysine coated or silane
treated slides. Prepare thin slices oftissue sections 4 μm in thickness.
Place the section onto the slide ataround the center. After drying at
37℃ for 24 hours, submit the slides.

Lung cancer ALK
protein,(IHC)
D5F3Lung cancer ALK
gene,
FISH

SAMPLE HANDLING METHOD

unstained
3 slidesUse coated slides such as silanized slides, and leave thinly-sliced
samples to dry overnight at about 40℃ before submitting them.
Prepare thin slices of tissue sections 4 to 6 μm in thickness. In
the case of baking for the purpose of preventing tissues fromcoming off, treat them for about 2 to 24 hours.

container handling method
CONTAINER FORM

Z10TEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Melanoma
PD-L1 protein,(IHC)28-8

Previous container symbolt[Object cases]
Preparation(slide glass)
Storageconditions:Roomtemperature

Head and neckcancer PD-L1
protein, IHC,
22C3

unstained
4 slides eachHead and neckcancer PD-L1
protein, (IHC)
28-8

CCR4 protein,
IHC

6 unstained slides

SAMPLE HANDLING METHOD

Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃ before
submitting them.
Thinly slice the tissue into 4 to 5 μm
and apply it onto the slide at thecenter, ≥ 15 mm from the frosted edgeand ≥ 15 mm from the slide glass edge.
Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃ before
submitting them. Slice the tissue tothe thickness of 4-5μm, and apply it
onto the slide at around the center.
R
Use coated slides such as silanizedslides, and leave thinly-sliced samples
to dry overnight at about 40℃ before
submitting them.
Thinly slice the tissue into 4 to 5 μm
and apply it onto the slide at thecenter, ≥ 15 mm from the frosted edgeand ≥ 15 mm from the slide glass edge.
For a testing material, provide unstained slides preparedusing paraffin blocks that have been fixed in 10–20% neutral
buffered formalin fixative for about 24 to 48 hours before
embedding. Thinly slice the samples into 3 to 4 μm, use coatedslides such as silanized slides, and leave thinly-sliced samples
to dry overnight at about 40℃ before submitting them.

container handling method�container handling method
Points to note concerning the submission of samples for gene-related tests.

CONTAINER FORM

Z10TEST NAME
EZH2 gene mutational analysis

UNSTAINED SLIDE THICKNESS RATE OFSTORE(NUMBER)(μm) TUMOR CELL TEMPERATURE

５

5

≥ 15％

BRAF V600 mutation analysis, PCR

５-10

10

≥ 50％

EGFR mutation analysis v2.0

５-10

10

≥ 10％

EGFR gene mutational analysis(Scorpion-ARMS technique)

５-10

5-10

≥ 10％

5

5

≥ 30％

ArcherMETex14 skipping, FFPE

５-10

5

≥ 10％

KRAS G12C mutational analysis

５

5

≥ 20％

IDH1/2 gene analysis (Glioma) (FFPE)

5-10

4-10

≥ 20％

RAS/BRAF gene mutational analysis

5

5-10

≥ 10％

Previous container symbolt[Object cases]
Preparation(slide glass)
Storageconditions:Roomtemperature

Qualitative detection of ROS1 genefusions (FFPE)

R

COLLECTING METHOD●Condition for submission

Perform histopathological assessment on unstained sample slides, and confirm that a sufficient proportion of tumor
cells are available for the testing.
If the proportion of tumor cells is not sufficient, mark the tumor cell region on the back side of the unstained sample
slide.
Please note that submission of unmarked slides leads to impact on the test result (e.g., fasle negativity) due toinability to perform micro-dissection.
For microsatellite instability (MSI) test (FFPE) and microsatellite instability (MSI) test (Lynch syndrome), tumor partand normal part must be distinguished because of the characteristics of the test. Be sure to mark the tumor cellregion.
However, FoundationOne CDx Cancer genome profile and myChoice diagnostic system are not included.

●Unstained sample slide

container handling method

Immediately immerse the collected tissue in 10% formalin neutral buffer solution for fixation (recommended durationof fixation: 6-48 hours It takes 18-36 hours for surgical material of ArcherMETex14 skipping (FFPE); 6-72 hours for
myChoice diagnostic system; and 3-6 hours for biopsy specimen).
Wherever possible, continuously slice the formalin fixed and paraffin embedded (FFPE) block produced in the past 3
years at a designated thickness to prepare serial sections. However, the AmoyDx Lung Cancer Multipanel should besubmitted with FFPE tissue prepared within 3 months, 3 to 12 months, and 12 to 24 months, respectively.
For submission, pay thorough precaution against contamination by changing microtome blades for each specimen.
The DNA has undergone fragmentation due to formalin-fixed tissues. Therefore, please note that an analysis may not
be possible depending on the conditions and period of storage, as well as the type and composition of a fixing solution
and fixation time.

●Biopsy sample

Make sure to handle biopsy specimen with special care as only a trace amount of specimen is extracted, and in somecases most tissues may not be present, or they may not include tumor cells.

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Points to note concerning the submission of samples for gene-related tests.

CONTAINER FORM

Z10
Previous container symbolt[Object cases]
Preparation(slide glass)
Storageconditions:Roomtemperature

TEST NAMEUNSTAINED SLIDE THICKNESS RATE OFSTORE(NUMBER)(μm) TUMOR CELL TEMPERATURE

Microsatellite instability (MSI) test
(FFPE)

５-10

5

≥ 50％

Oncomine Dx Target Test Multi-CDx(FFPE)

5-10

5

≥ 30％

OncomineDxTT Multi-CDx(thyroid cancer) FFPE

5-10

5

≥ 30％

Oncomine Dx Target Test Multi-for
research 46genes analysis (FFPE)

5-10

5

≥ 30％

OncomineDxTT Multi-for research(thyroid cancer) FFPE

5-10

5

≥ 30％
R

AmoyDx Lung Cancer Multipanel IVD
Specimen storage period: (less than 3months, 3-12 months, 12-24 months)

7-10 each

5 each≥ 20％(recommended ≥ 30%)

AmoyDx Lung Cancer Multipanel for
research
Specimen storage period: (less than 3months, 3-12 months, 12-24 months)

7-10 each

5 each≥ 20％(recommended ≥ 30%)

Lung cancer compact panel CDx

5-10

5

≥ 10％

Lung cancer compact panel for
research

5-10

5

≥ 10％

NCC Oncopanel System

5

10

≥ 20％

FoundationOne CDx Cancer genome
profile

10

4-5

≥ 30％

myChoice diagnostic system

8

5

≥ 20％

５-10

5

≥ 50％

Microsatellite instability (MSI) test
(Lynch syndrome)

R

R

COLLECTING METHOD●Condition for submission●Unstained sample slide

Immediately immerse the collected tissue in 10% formalin neutral buffer solution for fixation (recommended durationof fixation: 6-48 hours It takes 18-36 hours for surgical material of ArcherMETex14 skipping (FFPE); 6-72 hours for
myChoice diagnostic system; and 3-6 hours for biopsy specimen).
Wherever possible, continuously slice the formalin fixed and paraffin embedded (FFPE) block produced in the past 3
years at a designated thickness to prepare serial sections. However, the AmoyDx Lung Cancer Multipanel should besubmitted with FFPE tissue prepared within 3 months, 3 to 12 months, and 12 to 24 months, respectively.
For submission, pay thorough precaution against contamination by changing microtome blades for each specimen.
The DNA has undergone fragmentation due to formalin-fixed tissues. Therefore, please note that an analysis may not
be possible depending on the conditions and period of storage, as well as the type and composition of a fixing solution
and fixation time.

●Biopsy sample

Make sure to handle biopsy specimen with special care as only a trace amount of specimen is extracted, and in somecases most tissues may not be present, or they may not include tumor cells.

�

container handling method

Perform histopathological assessment on unstained sample slides, and confirm that a sufficient proportion of tumor
cells are available for the testing.
If the proportion of tumor cells is not sufficient, mark the tumor cell region on the back side of the unstained sample
slide.
Please note that submission of unmarked slides leads to impact on the test result (e.g., fasle negativity) due toinability to perform micro-dissection.
For microsatellite instability (MSI) test (FFPE) and microsatellite instability (MSI) test (Lynch syndrome), tumor partand normal part must be distinguished because of the characteristics of the test. Be sure to mark the tumor cellregion.
However, FoundationOne CDx Cancer genome profile and myChoice diagnostic system are not included.

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CONTAINER FORMTEST NAMEAMOUNT COLLECTED AMOUNT SUBMITTED STORETEMPERATURE
(mL)
(mL)

Z15
Previous container symbolt5

OncotypeDX
DCIS

15 slides in total(Refer to Preparing
HE stained andunstained sample
slides)OncotypeDX
Colon

OncotypeDX Kit Box
Storage conditions:Room temperature

CONTAINER FORM

Z50

Storage conditions: Room temperature

Previous container symbol

L5

container handling method

Container set



SAMPLE HANDLING METHOD(1) Please select the mostrepresentative tumor block.
(2) Thinly slice 15 tissue sections of 5μm thickness for RNA extraction.
(3) When picking up the tissue
sections, arrange them so thatthey are all in the same directions.
(4) Leave the sections that have been
picked up to dry naturally, avoid
paraffin melting.
(5) Please be sure to enter yourfacility’s specimen control number(any alphanumeric number) on theslide.
※Specimen control numbers should
be assigned by your facility.
Please enter the same specimenRcontrol number on each slide.
(6) Please be sure to write thesequence numbers 1 through 15 on
the slides in the order the sections
were sliced off.
(7) Attach the barcode attached to
the OncotypeDX Kit Box to eachslide.
※When preparing samples, pleasetake the following precautions to
avoid contamination:· Replace the microtome blades (ornew blades) for every sample.
· Use clean water when picking up
the sections.
· When slicing off thin sections,do not handle samples with barehands but use disposable gloves.