Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plantsystems
U Roessner, A Luedemann, D Brust, O Fiehn… - The Plant …, 2001 - Am Soc Plant Biol

Metabolic profiling using gas chromatography–mass spectrometry technologies is a technique whose potential in the field of functional genomics islargely untapped. To demonstrate the general usefulness of this technique, we applied to diverse plant genotypes …
Cited by 941

Measuring the metabolome: current analytical technologies
WB Dunn, NJC Bailey, HE Johnson - Analyst, 2005 - pubs.rsc.org
The post-genomics era has brought with it ever increasing demands to observe and characterise variation within biological systems. This variation
has been studied at the genomic (gene function), proteomic (protein regulation) and the metabolomic (small …
Cited by 806

Systems level studies of mammalian metabolomes: the roles of mass spectrometry and nuclear magnetic
resonance spectroscopy
WB Dunn, DI Broadhurst, HJ Atherton… - Chemical Society …, 2011 - pubs.rsc.org

The study of biological systems in a holistic manner (systems biology) is increasingly being viewed as a necessity to provide qualitative andquantitative descriptions of the emergent properties of the complete system. Systems biology performs studies focussed on the …
Cited by 457[HTML] The human urine metabolome

S Bouatra, F Aziat, R Mandal, AC Guo, MR Wilson… - PloS one, 2013 - journals.plos.org
Urine has long been a “favored” biofluid among metabolomics researchers. It is sterile, easy-to-obtain in large volumes, largely free from interfering
proteins or lipids and chemically complex. However, this chemical complexity has also made urine a particularly difficult …
Cited by 481

Metabolomics: from pattern recognition to biological interpretation
W Weckwerth, K Morgenthal - Drug discovery today, 2005 - Elsevier
Metabolomics is a technology that aims to identify and quantify the metabolome–the dynamic set of all small molecules present in an organism or abiological sample. In this sense, the technique is distinct from metabolic profiling, which looks for target compounds …
Cited by 278

Construction and application of a mass spectral and retention time index database generated from plant
GC/EI-TOF-MS metabolite profiles
C Wagner, M Sefkow, J Kopka - Phytochemistry, 2003 - Elsevier

The non-supervised construction of a mass spectral and retention time index data base (MS/RI library) from a set of plant metabolic profiles coveringmajor organs of potato (Solanum tuberosum), tobacco (Nicotiana tabaccum), and Arabidopsis thaliana, was …
Cited by 243[HTML] Quantitative metabolomics based on gas chromatography mass spectrometry: status andperspectives
MM Koek, RH Jellema, J van der Greef, AC Tas… - Metabolomics, 2011 - Springer
Metabolomics involves the unbiased quantitative and qualitative analysis of the complete set of metabolites present in cells, body fluids and tissues(the metabolome). By analyzing differences between metabolomes using biostatistics (multivariate data analysis; pattern …
Cited by 219

Metabonomics classifies pathways affected by bioactive compounds. Artificial neural network classification ofNMR spectra of plant extracts
KH Ott, N Aranıb
́ ar, B Singh, GW Stockton - Phytochemistry, 2003 - Elsevier
The biochemical mode-of-action (MOA) for herbicides and other bioactive compounds can be rapidly and simultaneously classified by automatedpattern recognition of the metabonome that is embodied in the 1 H NMR spectrum of a crude plant extract. The ca. 300 …
Cited by 195

[CITATION] Metabolic profiling: a Rosetta Stone for genomics?
RN Trethewey, AJ Krotzky, L Willmitzert - Current Opinion in Plant Biology, 1999 - Elsevier
Man has always aspired to understand the nature of life and the chances of achieving this, at least at the molecular level, have never looked better.
As a technolog) i-driven revolution sweeps through the life sciences and we are submerged in an avalanche of new information …
Cited by 200

High-resolution metabolic phenotyping of genetically and environmentally diverse potato tuber systems.
Identification of phenocopies
U Roessner, L Willmitzer, AR Fernie - Plant physiology, 2001 - Am Soc Plant Biol
We conducted a comprehensive metabolic phenotyping of potato (Solanum tuberosum L. cv Desiree) tuber tissue that had been modified either bytransgenesis or exposure to different environmental conditions using a recently developed gas chromatography-mass …
Cited by 194

Principal component analysis of urine metabolites detected by NMR and DESI–MS in patients with inbornerrors of metabolism
Z Pan, H Gu, N Talaty, H Chen, N Shanaiah… - Analytical and …, 2007 - Springer

Urine metabolic profiles of patients with inborn errors of metabolism were examined withnuclear magnetic resonance (NMR) and desorption electrospray ionization mass spectrometry (DESI–MS) methods. Spectra obtained from thestudy of urine samples from …
Cited by 163

Metabolic Profiling of Root Exudates of Arabidopsis thaliana
TS Walker, HP Bais, KM Halligan… - Journal of Agricultural …, 2003 - ACS Publications
This paper was withdrawn on October 7, 2009 (J. Agric. Food Chem. 2009, 57, 9346). In addition to accumulating biologically active chemicals, plantroots continuously produce and secrete compounds into their immediate rhizosphere. However, the mechanisms that drive …
Cited by 140[BOOK] Metabolome analysis: an introduction
SG Villas-Boas, J Nielsen, J Smedsgaard… - 2007 - books.google.com
Providing information on the main approaches for the analysis of metabolites, this textbook: Covers basic methodologies in sample preparation andseparation techniques, as well as the most recent techniques of mass spectrometry. Differentiates between primary and …
Cited by 143

Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass
spectrometry
S Strelkov, M von Elstermann, D Schomburg - Biological Chemistry, 2004 - degruyter.com
An analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation of Corynebacterium
glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than …
Cited by 129

Diagnosis of inborn errors of metabolism using filter paper urine, urease treatment, isotope dilution and gas
chromatography–mass spectrometry
T Kuhara - Journal of Chromatography B: Biomedical Sciences …, 2001 - Elsevier

This review will be concerned primarily with a practical yet comprehensive diagnostic procedure for the diagnosis or even mass screening of a variety
of metabolic disorders. This rapid, highly sensitive procedure offers possibilities for clinical chemistry laboratories to …
Cited by 106

Gas chromatographic–mass spectrometric urinary metabolome analysis to study mutations of inborn errorsof metabolism
T Kuhara - Mass spectrometry reviews, 2005 - Wiley Online Library
Urine contains numerous metabolites, and can provide evidence for the screening or molecular diagnosis of many inborn errors of metabolism(IEMs). The metabolomic analysis of urine by the combined use of urease pretreatment, stable-isotope dilution, and capillary …
Cited by 100

Diagnosis and monitoring of inborn errors of metabolism using urease-pretreatment of urine, isotope dilution,and gas chromatography–mass spectrometry
T Kuhara - Journal of Chromatography B, 2002 - Elsevier

To diagnose inborn errors of metabolism, it would be desirable to simultaneously analyze and quantify organic acids, purines, pyrimidines, aminoacids, sugars, polyols, and other compounds using a single-step fractionation; unfortunately, no such method currently exists …
Cited by 94

Gene discovery via metabolic profiling
RN Trethewey - Current Opinion in Biotechnology, 2001 - Elsevier
Biochemical analysis is adding a new dimension to the process of gene discovery. Two major developments have recently taken place in theemerging science of biochemical genomics. The first is an approach that uses a combination of tagged fusion proteins and …
Cited by 87

Metabolic profiling and biochemical phenotyping of plant systems
U Roessner, L Willmitzer, A Fernie - Plant Cell Reports, 2002 - Springer
Over the last two decades incredible progress has been made in the development of technologies to both create and characterise genetic diversity. In
plant systems, the development of knockout populations, transposon insertions and chemical mutagenesis …
Cited by 83Simplified screening for organic acidemia using GC/MS and dried urine filter paper: a study on neonatal
mass screening
X Fu, M Iga, M Kimura, S Yamaguchi - Early human development, 2000 - Elsevier

A simplified method for organic acidemia screening using GC/MS, the urease/direct method, is now available. To establish a practical screening
system for organic acidemias, we studied the usefulness of dried urine filter paper (filter paper urine) and the application of a …
Cited by 74

Automated metabolic profiling and interpretation of GC/MS data for organic acidemia screening: a personal
computer-based system
M Kimura, T Yamamoto, S Yamaguchi - The Tohoku journal of …, 1999 - jstage.jst.go.jp
We have developed a personal computer-based system designed for automated metabolic profiling of urinary organic acids by gas
chromatography-mass spectrometry (GC/MS) and data interpretation for organic acidemia screening. For the automated profiling …
Cited by 74

Pilot study of gas chromatographic–mass spectrometric screening of newborn urine for inborn errors ofmetabolism after treatment with urease
T Kuhara, T Shinka, Y Inoue, M Ohse… - … of Chromatography B …, 1999 - Elsevier
Gas chromatographic–mass spectrometric (GC–MS) techniques for urinary organic acid profiling have been applied to high-risk screening for a wide
range of diseases, mainly for inborn errors of metabolism (IEM), rather than to low-risk screening or mass screening …
Cited by 66

Noninvasive human metabolome analysis for differential diagnosis of inborn errors of metabolism
T Kuhara - Journal of Chromatography B, 2007 - Elsevier
Early diagnosis and treatment are critical for patients with inborn errors of metabolism (IEMs). For most IEMs, the clinical presentations are variable
and nonspecific, and routine laboratory tests do not indicate the etiology of the disease. A diagnostic procedure using …
Cited by 61

Automated mode-of-action detection by metabolic profiling

N Araníbar, BK Singh, GW Stockton, KH Ott - Biochemical and Biophysical …, 2001 - Elsevier
Rapid classification and identification of the mode-of-action of bioactive compounds applied to plants can be achieved by a robust and easy-to-use
metabolic-profiling method. This method uses artificial neural network analysis of one-dimensional proton NMR spectra of …
Cited by 59

Computational strategies for metabolite identification in metabolomics
DS Wishart - Bioanalysis, 2009 - Future ScienceMost metabolomic data are characterized by complex spectra or chromatograms containing hundreds of peaks or features. While there are manymethods for aligning or comparing these spectral features, there are few approaches for actually identifying which peaks match …
Cited by 60

Determination of the spectrum of low molecular mass organic acids in urine by capillary electrophoresis withcontactless conductivity and ultraviolet photometric …
P Tůma, E Samcová, K Štulík - Analytica chimica acta, 2011 - Elsevier
A mixture of 29 organic acids (OAs) occurring in urine was analyzed by capillary electrophoresis (CE) with capacitively coupled contactless
conductivity detection (C 4 D) and UV photometric detection. The optimized analytical system involved a 100 cm long …
Cited by 57

Sarcosine as a marker in prostate cancer progression: a rapid and simple method for its quantification inhuman urine by solid-phase microextraction–gas …
B Cavaliere, B Macchione, M Monteleone… - Analytical and …, 2011 - Springer

Sarcosine is an amino acid derivative of N-methylglycine and is involved in the amino acid metabolism and methylation processes that are enrichedduring prostate cancer progression. It could also serve as a new target to be measured during therapeutic …
Cited by 48