Blisters
Efficient long-term liquid reagent storage
on lab-on-a-chip devices
Storage of liquid reagents on a microfluidic device is oftentimes
a prerequisite for successful lab-on-a-chip applications. The useof blister pouches is not only a convenient way to incorporate
liquids on-chip but also ensures long-term storage capability.
Blister pouches serve as storage vessels and at the same timeenable liquid movement through the application of pressure ina defined manner.

Off-The-Shelf Blister Pouches• 50 µl - 750 µl filling volumes• Compatible with inorganic solutions, acids andbases• Reliable long-term liquid reagent storage• Minimized contamination risk
Blister Test Chips• Various off-the-shelf designs to evaluate blister
emptying performance• Feature microfluidic ChipShop‘s unique piercing
blister seat technology for controlled liquid
release
ChipGenie® edition BD• High-precision blister driver instrumentation• Compatible with most of microfluidic ChipShop‘soff-the-shelf blister test chips• Controlled blister actuation operated with userfriendly control software and visual control unit
Customer-Specific Solutions & Services• Blister filling service for customer-specific reagents• Complex microfluidic cartridge development withcomprehensive functional integration incl. liquidand dry reagent storage on-chip

Contact in Japan: ASICON Tokyo Ltd.
www.asicon-tokyo.com / asicon-tokyo@asicon-tokyo.com

Blisters - Liquid Reagent Storage

Blister Test Chip - Small Volume
microfluidic ChipShop offers a multitude of microfluidic chips to evaluate blister emptying. The blister test chip Fluidic 289 isparticularly suited to investigate blisters with small volumes and comes with two blisters of 50 µl and 100 µl liquid content. For
convenient visual assessment, both blisters are filled with dyed water.

Blister test chip with small volume blisters - blisters contain 50 µl and100 µl of dyed water

Schematic drawing of small volume blister test chip Fluidic 289
with two blister seats and metering system - view from top

Schematic cross section of a standard blister setup on a microfluidic
deviceProduct Codefor Fluidic 289

Description

Blister details

Lid thickness [µm]Surfacetreatment

Material

10001305

Blister test chip
Small volume

Blister position 1: 50 µl; cyan; H2O
Blister position 2: 100 µl; magenta; H2O

140-Topas

10001306

Blister test chip
Small volume

Blister position 1: 50 µl; cyan; H2O
Blister position 2: 100 µl; magenta; H2O

140

hydrophilized

Topas

Blister Test Chip - Volume Evaluation
Blister test chip Fluidic 522 comes with two mounted 250 µl blisters, each of which is adjoined to a metering system. The blister
emptying can be assessed with the aid of dyed blister content and either a chamber metering (blister position 1) or a channel
metering system (blister position 2).

Blister test chip Fluidic 522 for volume evaluation - blisters contain
250 µl of dyed water

Schematic drawing of blister test chip Fluidic 522 with two blisterseats and metering systems - view from top

General components of a microfluidic chip exemplary shown byblister test chip Fluidic 522. The product consists of a chip body,
attached blisters and a bonded cover lid

Schematic drawing of blister test chip Fluidic 522 – view frombottom (metering systems)

Close-up of blister seat with needles to pierce the bottom blister foil

Product Codefor Fluidic 522

Description

Blister details

Lid thickness [µm]Surfacetreatment

Material

10001307

Blister test chipVolume evaluation

Blister position 1: 250 µl; cyan; H2O
Blister position 2: 250 µl; magenta; H2O

140-Topas10001308

Blister test chip
Volume evaluation

Blister position 1: 250 µl; cyan; H2O
Blister position 2: 250 µl; magenta; H2O

140

hydrophilizedTopas

Blisters - Liquid Reagent Storage

Blister Test Chip - Volume Variation
This blister test chip is suitable for evaluating the emptying of a variety of blisters with volumes ranging from 50 µl to 500 µl. Thechip design allows exact metering of 5 µl (for 50 µl blister), 15 µl (for 150 µl blister) and 25 µl (for 250 µl and 500 µl blisters)with the aid of downstream channel/chambers.

Blister test chip Fluidic 1021 with blister pouches of various volumes. Each blister is associated to an appropriate metering system

Schematic drawing of blister test chip Fluidic 1021 with meteringsystems – view from top (blister seats and fluidic interfaces)

Product CodeDescription
for Fluidic 1021

Blister details

Lid thick- Surface
ness [µm] treatment

Material

10001309

Blister test chip- Volume variation

Blister position 1: 500 µl; magenta; H2O
Blister position 2: 250 µl; cyan; H2OBlister position 3: 150 µl; magenta; H2O
Blister position 4: 50 µl; cyan; H2O

140Topas

Blister test chip
- Volume variation

Blister position 1: 500 µl; magenta; H2O
Blister position 2: 250 µl; cyan; H2O
Blister position 3: 150 µl; magenta; H2O
Blister position 4: 50 µl; cyan; H2O

14010001310-hydrophilizedTopas

Schematic drawing of blister test chip Fluidic 1021 with meteringsystems – view from bottom (metering system)

Did you know?
Controlled blister bursting
microfluidic ChipShop is using aunique blister seat technology.
Thanks to piercing needles theblister content is released in acontrolled and even manner. Anerratic bursting of the blister istherefore obviated.

ChipGenie® edition BD
microfluidic ChipShop’s blister driver ChipGenie® edition BD is the tailored instrument for evaluation of blister emptying
behavior. The device has been specifically developed to be used with microfluidic ChipShop‘s microscope slide-format blister test
chips, such as Fluidic 289 or Fluidic 522. The instrument ensures both a convenient, user-friendly chip insertion and a precise
positioning of the inserted blister chip. Blister emptying is facilitated by two independent, high-precision vertical blister drives,
while an integrated camera gives immediate visual feedback.
Instrument features:• Two independent, high-precision vertical blister drives (driver speed 0.1 µm/s up to 200 µm/s; positioning accuracy +/- 20 µm)• Integrated camera for direct visual feedback• Includes user-friendly software for easy system control• Connection to PC established via USB port

Blisters of a blister test chip being actuated by the ChipGenie® edition
BD instrument

Schematic succession of blister actuation by means of the ChipGenie® edition BDinstrument

Product CodeDescription

Dimensions[cm]

10000686

ChipGenie® edition BD – Blister Driver instrument

20 x 12 x 11

Blisters - Liquid Reagent Storage

Spare Blister Pouches
Blisters can be ordered as stand-alone parts being available with volumes ranging from 50 µl to 750 µl off-the-shelf. All blisters
come with matching double-sided adhesive tape for convienent mounting on the microfluidic device. After use, blisters can beeasily removed and replaced. All blisters can be ordered filled either with dye (cyan, magenta, yellow) or water (clear).
If you are interested in blisters filled with your customer-specific reagents please inquire our custom filling service. Simply write
an e-mail with your requirements to inquiries@microfluidic-ChipShop.com.

1.3

1.5

1.1

1.3

0.9

1.1

0.7

0.9

0.5

0.7

0.3

0.5

0.1

0.3

Volume Loss [% Total Volume]

Volume Loss [% Total Volume]

1.5-0.1 0 0.1
-0.3

-0.1

4°C
20°C
37°C
100

14200300

202840086 Time [d]Storage

500120600

177700

481

800

797

Storage Time [d]Individual blister pouches with different filling volumes

Storage time dependent volume loss assessed for three different storage temperatures - blisters with 200 µl ddH2O filling were stored at 4 °C,
20 °C and 37 °C and volumes were monitored over a time period of more than two years. At room temperature blister filling loss was less than
0.2% over 797 days

Product code (color-dependent)

Description

Blister
Volume [µl]Cyan

Magenta

Yellow

Clear

10001605

10001606

10001607

10001608

Blister pouch

50

10001610

10001611

10001612

10001613

Blister pouch

100

10001615

1000161610001617

10001618

Blister pouch

150

10001620

10001621

10001622

10001623

Blister pouch

200

10001597

10001537

10001538

10001625

Blister pouch

250

10001602

10001601

10001600

10001599

Blister pouch

350

10001603

10001626

10001627

10001628

Blister pouch

500

10001665

10001666

10001667

10001668

Blister pouch

750

Did you know?
Liquid storage in tanks
Blisters are not the only way
liquid reagents can be stored in
microfluidics. In fact, for large
volumes and short-term storage theuse of tanks might be advisable.
To learn more visit our webpage orhave a look at our catalogue andour „Accessories“ leaflet!

Tailored Blister Solutions
microfluidic ChipShop offers a variety of services on the subject of blister pouches. Looking to fill our standard blister pouches
with reagents specific to your experiment? Simply inquire our blister filling service.
microfluidic ChipShop is also your specialized partner when it comes to the development of blister-containing microfluidic
cartridges - from relatively simple customer-specific blister test chips to sophisticated, fully integrated lab-on-a-chip platforms.
No matter how basic or complex your microfluidic assay requirements are, please contact us with your idea atinquiries@microfluidic-ChipShop.com!

Simple blister test chip to evaluate blister emptying of a blister with alarger volume of 500 µl. This test chip with Fluidic 761 is available ascatalogue product with microfluidic ChipShop

Blister of different sizes mounted on a complex microfluidic cartridge,
which has been developed at microfluidic ChipShop and features a
great variety of functional units

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Contact in Japan ASICON Tokyo Ltd.
www.asicon-tokyo.com / asicon-tokyo@asicon-tokyo.com

A sophisticated lab-on-a-chip device with mounted blister, which areprotected by a cover from unintentional actuation