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Product Name: DynaMarker® DNA Small
Code No. : DM100
Packing Size: 7 μg (50 μl), approximately 50 loadings

This product is for research use only
Description: DynaMarker® DNA Small consists of 20, 30, 40, 50, 60, 75 and 100 bp of blunt-end dsDNA.
The order and size of each fragment is easy to distinguish and the marker shows very sharp bands. TheDNA size marker is ideal for sizing small PCR fragments on non-denaturing acrylamide gelelectrophoresis. It is possible to use

DynaMarker®

DNA Small on high-concentration agarose gelelectrophoresis but acrylamide gel electrophoresis provides high-resolution separation of small DNA
fragments (less than 100 bp).

Storage condition:-20℃

Storage buffer:

10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM EDTA

Recommended Loading:Supplied product:

1 l / lane

6 × BPB loading dye (EDTA, glycerol and bromphenol blue are contained)band size (bp)Electrophoresis of DynaMarker® Small DNA

100Gel: Non-denaturing acrylamide gel (10%)

756050Loading: DynaMarker® Small DNA, 1 μl

40After electophoresis, electrophoresed gel was stained30with 0.5 μg/ml of EtBr for 15 min.

Running buffer: 1 × TBE
Time and voltage: 200V, 20 min

201

BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.2

Recommended usage:
Small DNA fragments (approximately 20 to 100 bp) are often separated on 10-15% of non-denaturing
polyacrylamide gel electrophoresis. For example,

DynaMarker®

DNA Small can be run on 10 %non-denaturing polyacrylamide gel as below.
1. Preparation of 10 % polyacrylamide gel(20 ml gel)

40 % acrylamide : bis solution (19:1)

5.0 ml10 × TBE

2.0 ml

H2O

to 20 ml

2. After mixing reagents described above, add 20 l of TEMED and 160 l of 10 % ammonium persulfate.
Mix quickly and then pour the gel into the mold of a vertical gel apparatus (20 ml is enough gel solution
for two 7 cm × 8 cm, thickness 0.1 cm gels). The gel apparatus should be assembled according to themanufacturer’s protocol and ready to run with 1 × TBE buffer.
3. Loading and electrophoresis
Prepare DNA sample for electrophoresis as below.
1)

Size Marker:
DynaMarker®

2)

DNA Small

1 ldistilled water

4 l

6 × BPB loading dye

1 l

Sample to examine:DNA sample

5 l

6 × BPB loading dye

1 l

Mix the DNA solution with 6 × BPB loading dye in a tube as above. Load the mixture onto a well of
10 % non-denaturing polyacrylamide gel and start electrophoresis. After the tracking dye has migrated anappropriate distance through gel, stop the electrophoresis. To stain with ethidium bromide, disassemble
the apparatus and transfer the polyacrylamide gel to a gel tray filled with distilled water containing 0.5
g/ml ethidium bromide for approximately 15 minutes*. The stained DNA can be visualized using UVtransilluminator.
* Longer staining time may decrease the DNA band intensity because small DNA diffuses within the gelover time.

Related products:
DynaMarker®

DNA Low D (#DM112): for determining the size of low size DNA (50 to 1,000 bp)

DynaMarker®

DNA High D (#DM122): for determining the size of high size DNA (300 to 10,000 bp)

DynaMarker®

for Plasmid D (#DM132): for determining the size of high size DNA (200 to 7,000 bp)

2

BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.2