ACCESS™ WORKSTATION CONFIGURATION

Access Laboratory Workstation forCell-based and Biochemical Assays

ACCESS WORKSTATION CONFIGURATION | Automated Cell-based and Biochemical Assays

A102

ACCESS™ WORKSTATION CONFIGURATION

Automated Cell-based and Biochemical Assays

Examples of Access Workstation Configurations forfor Cell-based and Biochemical Assays
Echo® Liquid Handlers use acoustic energy to provide high-throughput, non-contact liquid handling for a range of applications. Non-contact
transfer avoids the risk of cross-contamination, eliminates tip costs, and facilitates the progression to high density assay formats. Echo LiquidHandlers precisely and accurately transfer 2.5 nL or 25nL droplets of sample, cells, and reagent, enabling the miniaturization of both cellbased and biochemical screens that can reduce operating costs without compromising data quality.
The Access™ Laboratory Workstation combines the revolutionary performance of the Echo Liquid Handler with automated plate handlingand integrated devices to create walk-away systems tailored for a range of applications in need of higher throughput. Access systems aremodular, flexible solutions that can scale when needed. With use of the Access Workstation, Echo Liquid Handlers can be easily incorporated
into an integrated system to automate workflows including, but not limited to, cell dispensing, cell proliferation and viability assays, gene
expression assays, potency and efficacy assays, and transfection workflows.

CONFIGURATION AXPeel

SYSTEM COMPONENTS
Echo 525● ACC-700 Access Workstation Base System● ACC-706 2D Barcode Reader● ACC-708 25-Plate HD Random Access Rack

PlateLocSealer● ACC-710 20-Plate Random Access Racks● ACC-711 50-Plate Sequential Stacker● ACC-712 4-Plate Lid Handling Station● ACC-714 Base Extension with Shelf

Combi nL

● ACC-715 96” Workstation Table

Echo 555● ACC-725 Rear Echo Table

SYSTEM DEVICES● Labcyte Echo 525, 550, or 555 Liquid Handler● ThermoFisher Multidrop Combi nL● Agilent Technologies PlateLoc Sealer● Agilent Technologies Microplate Centrifuge● Brooks Automation XPeel Peeler

Storage Racks2

LABCYTE INC.

CONFIGURATION B
BioTek MultiFlo FX Bulk Dispenser● RAD Single Channel Dispenser● 1 Peristaltic 8-channel Dispenser● 2 Syringe 8-channel Dispensers

PE Envision
Multimode Plate Reader

BioTek EL406 Washer● 96- or 192-pin Manifold● 1 Peristaltic 8-channel Dispenser

Cytomat 10C Robotic Incubator● 2 Syringe Pump 8-channel Dispensers● 280-, 1536-well Plates● 210-, 96-, or 384-well Plates

SYSTEM COMPONENTSSYSTEM DEVICES● ACC-700 Access Workstation Base System● Labcyte Echo 525 or 555 Liquid Handler● ACC-708 25-Plate HD Random Access Rack

● BioTek MultiFlo FX Full-loaded with RAD Tech

● ACC-706 2D Barcode Reader● ACC-710 20-Plate Random Access Racks● ACC-711 50-Plate Sequential Stacker● ACC-712 4-Plate Lid Handling Station● ACC-714 Base Extension with Shelf● ACC-715 96” Workstation Table● ACC-719 Large Rear Table● ACC-727 Large Rear Table Shelf● ACC-724 Small Rear Table

CFG-A102-1.0● BioTek EL406 Fully-loaded with 96- or 192-pin Manifold● Inheco Teleshake● ThermoFisher Multidrop Combi nL● Agilent Technologies PlateLoc Sealer● Agilent Technologies Microplate Centrifuge● Brooks Automation XPeel Peeler● ThermoFisher Cytomat 10C Series Incubator● PerkinElmer Envision Multimode Plate Reader

3ACCESS™ WORKSTATION CONFIGURATION

Automated Cell-based and Biochemical Assays

CONFIGURATION CBMG PHERAstar Multimode Plate Reader

Agilent PlateLoc and MultiDrop Combi on Small Rear Table + Shelf

Combi nL

Cytomat 10C Robotic Incubator● 280-, 1536-well Plates● 210-, 96-, or 384-well Plates

SYSTEM COMPONENTSSYSTEM DEVICES● ACC-700 Access Workstation Base System● Labcyte Echo 525 or 555 Liquid Handler● ACC-708 25-Plate HD Random Access Rack

● Cytomat 10C Series Incubator● ACC-706 2D Barcode Reader● ACC-710 20-Plate Random Access Racks● ACC-711 50-Plate Sequential Stacker● ACC-712 4-Plate Lid Handling Station● ACC-714 Base Extension with Shelf● ACC-715 96” Workstation Table● ACC-719 Large Rear Table● ACC-727 Large Rear Table Shelf● ACC-724 Small Rear Table● ACC-729 Shelf for Small Rear Table

4● BMG PHERAstar FS Plate Reader● Inheco Teleshake● ThermoFisher Multidrop Combi nL (2X)

● Agilent Technologies PlateLoc Sealer (2X)

● Agilent Technologies Microplate Centrifuge● Brooks Automation XPeel Peeler

LABCYTE INC.

CONFIGURATION DCombi nL

BMG PHERAstar Multimode Plate Reader

Cytomat 10C Robotic Incubator● 280-, 1536-well Plates● 210-, 96-, or 384-well Plates

BioTek EL406 Washer

SYSTEM COMPONENTSSYSTEM DEVICES● ACC-700 Access Workstation Base System● Labcyte Echo 525 or 555 Liquid Handler● ACC-708 25-Plate HD Random Access Rack

● Cytomat 10C Series Incubator● ACC-706 2D Barcode Reader● ACC-710 20-Plate Random Access Racks● ACC-711 50-Plate Sequential Stacker● ACC-712 4-Plate Lid Handling Station● ACC-714 Base Extension with Shelf● ACC-715 96” Workstation Table● ACC-719 Large Rear Table● ACC-727 Large Rear Table Shelf

CFG-A102-1.0● BMG PHERAstar FS Plate Reader● Inheco Teleshake● ThermoFisher Multidrop Combi nL● Agilent Technologies PlateLoc Sealer● Agilent Technologies Microplate Centrifuge● Brooks Automation XPeel Peeler● BioTek EL406 Fully-loaded with 96- or 192-pin Manifold

5ACCESS™ WORKSTATION CONFIGURATION

Automated Cell-based and Biochemical Assays

CONFIGURATION EBMG PHERAstar Multimode Plate Reader

2X Combi nL Dispensers

SYSTEM COMPONENTSSYSTEM DEVICES● ACC-700 Access Workstation Base System● Labcyte Echo 525 or 555 Liquid Handler● ACC-708 25-Plate HD Random Access Rack

● Inheco Teleshake● ACC-706 2D Barcode Reader● ACC-710 20-Plate Random Access Racks● ACC-711 50-Plate Sequential Stacker● ACC-712 4-Plate Lid Handling Station● ACC-714 Base Extension with Shelf● ACC-715 96” Workstation Table● ACC-724 Small Rear Table

6● BMG PHERAstar FS Plate Reader● ThermoFisher Multidrop Combi nL (2X)
● Agilent Technologies PlateLoc Sealer● Agilent Technologies Microplate Centrifuge● Brooks Automation XPeel Peeler

LABCYTE INC.

CONFIGURATION FPE Envision Multimode Plate Reader

Cytomat 2C Robotic Incubator● 56-, 1536-well Plates● 42-, 96-, or 384-well Plates

BioTek MultiFlo FX Bulk Dispenser● RAD Single Channel Dispenser● 1 Peristaltic 8-channel Dispenser● 2 Syringe 8-channel Dispensers

SYSTEM COMPONENTSSYSTEM DEVICES● ACC-700 Access Workstation Base System● Labcyte Echo 525 or 555 Liquid Handler● ACC-708 25-Plate HD Random Access Rack

● BioTek MultiFlo FX Full-loaded with RAD Tech

● ACC-706 2D Barcode Reader● ACC-710 20-Plate Random Access Racks● ACC-711 50-Plate Sequential Stacker● ACC-712 4-Plate Lid Handling Station● ACC-714 Base Extension with Shelf● ACC-715 96” Workstation Table● ACC-724 Small Rear Table

CFG-A102-1.0● Inheco Teleshake● PerkinElmer Envision Multimode Plate Reader● Agilent Technologies PlateLoc Sealer● Agilent Technologies Microplate Centrifuge● Brooks Automation XPeel Peeler● ThermoFisher Cytomat C2 Series

7

ACCESS™ WORKSTATION CONFIGURATION

Automated Cell-based and Biochemical AssaysCONFIGURATION GPE Envision Multimode Plate Reader

Roche LightCycler● 96/384 or 1536

Cytomat 10C Robotic Incubator● 280-, 1536-well Plates● 210-, 96-, or 384-well Plates

BioTek EL406 Washer● 96- or 192-pin Manifold● 1 Peristaltic 8-channel Dispenser● 2 Syringe Pump 8-channel Dispensers

SYSTEM COMPONENTSSYSTEM DEVICES● ACC-700 Access Workstation Base System● Labcyte Echo 525 or 555 Liquid Handler● ACC-708 25-Plate HD Random Access Rack

● BioTek EL406 Fully-loaded with 96- or 192-pin Manifold● ACC-706 2D Barcode Reader● ACC-710 20-Plate Random Access Racks● ACC-711 50-Plate Sequential Stacker● ACC-712 4-Plate Lid Handling Station● ACC-714 Base Extension with Shelf● ACC-715 96” Workstation Table● ACC-719 Large Rear Table● ACC-727 Large Rear Table Shelf

8● Inheco Teleshake● PerkinElmer Envision Multimode Plate Reader● Agilent Technologies PlateLoc Sealer● Agilent Technologies Microplate Centrifuge● Brooks Automation XPeel Peeler● Roche LightCycler 480 or 1536

LABCYTE INC.

Workflows
Cell Dispensing

Genomic Assays• Use either the integrated dispenser for plating cells in bulk orthe Echo 525 for dispensing high value samples• Dispense cells, with or without test molecule using the Echo orbulk dispenser• Incubate plate using ambient or temperature controlled storage
locations

Cell Modification• Dispense transfection reagents to assay plate using Echo orbulk dispenser• Add lysis buffer to cells using bulk dispenser or Echo
• Add primer, probe, or master mix using the Echo 525• Run xPCR or use a multimode reader to generate results• Transfer DNA, RNP, siRNA, or gRNA to assay plate using theEcho Liquid Handler• Dispense cells to transfection complex in assay plate using
Echo or bulk dispenser

Phenotypic Assays• Incubate at 37°C in the environment controlled incubator• Dispense cells, with or without test molecule in single, direct
dilution or combination formats• Use the integrated plate washer for media changes and cellmaintenance until ready for assay• Incubate using the environment controlled incubator• Use the plate washer as needed for cell washes and mediachanges

Potency / Efficacy Assays• Dispense cells onto compound ready assay plates or dispense
compounds, single or direct dilution range of concentrations,using Echo Liquid Handler into the cell plate• Add stain or detection reagent using an Echo or bulk dispenser• Incubate in ambient or temperature controlled storage locations• Read plate on integrated plate reader for results• Add lysis buffer or leave cells intact• Dispense detection reagent• Use ambient or temperature controlled storage for incubation
steps• Read plate and generate data using integrated multimodereader

CFG-A102-1.09

ACCESS™ WORKSTATION CONFIGURATION

Automated Cell-based and Biochemical Assays

Labcyte Microplates
Sample Storage

Protection• Labcyte Echo® Qualified 384 and 1536LDV Microplates aredesigned for long term sample storage with minimal deadvolume• Labcyte MicroClime™ Lids protect samples and reagents fromevaporation and contamination

Reagent Source• Labcyte Echo® Qualified 384PP Microplates and Echo® Qualified
Reservoirs are commonly used to contain assay reagents andcells during transfer

Other Compatible Echo Liquid Handlers
This Access Workstation is compatible with all 500 series of Echo Liquid Handlers.

Software Applications
The Access Workstation includes the advanced, but easy to use, Tempo™ scheduling software that can integrate Echo software protocols
into fully automated routines. Many Access Workstation users find Echo® Plate Reformat, Echo® Dose-Response, Echo® Cherry Pick and Echo®
Combination software essential for cell based and biochemical applications.

Information
For more information, please visit www.labcyte.com/access.

10

LABCYTE INC.

This page intentionally left blank.

CFG-A102-1.011

LABCYTE INC.
1190 Borregas AveSunyvale, CA 94089
USAToll-free: +1 877 742-6548 | Fax: +1 408 747-2010

SALES
North AmericaEuropeAsiaOther© 2017 LABCYTE INC. All rights reserved. Labcyte®, Echo®, MicroClime®, the Labcyte logo, Pod™, andAccess™ are r egistered trademarks or trademarks of Labcyte Inc., in the U.S. and/or other countries.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

| +1 408 747-2000| +353 1 6791464| +61 39018 5780
| +1 408 747-2000| info-us@labcyte.com| info-europe@labcyte.com| info-asia@labcyte.com| info-us@labcyte.com

CFG-A102-1.0
JAN 2017