Anti-HP1/CBX3 antibody, rabbit polyclonal, ChIP grade
Product code70-225

Size

50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenSynthetic peptide WHSCPEDEAQ-C to the C-terminal sequence of humanHP1γ (Ref 2, 3)

IsotypeRabbit IgG

Reactivity

Human and hamster. Expected to react with chicken, Xenopus, Drosophila, andzebra fish orthologs due to the sequence identity of the immunogen.

Special notes

N/A

Application

1. Western blotting (1/1,000~1/10,000) (Fig. 1 & Ref. 2)
2. Immunofluorescence staining (Fig.2, Ref. 2 & 3)
3. Chromatin immunoprecipitation (ChIP) (Ref. 3)

Background

Heterochromatin protein 1 (HP1) is a major component of heterochromatin
which plays a role in assembly of various proteins on chromatin and genesilencing. The HP1 family is evolutionally conserved, with members in fungi,
plants and animals but not prokaryotes, and there are multiple members within
the same species. The HP1 family proteins are encoded by a class of genes known
as the chromobox (CBX) genes. In humans, HP1γ is encoded by the Chromoboxhomolog 3 (CBX3) gene. HP1γ has been observed to interact directly orindirectly with several non-histone proteins with a wide variety of functions (Ref1).

Data Link

UniProtKB Q13185 (CBX3_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-225 Anti- HP1γ/CBX3 antibody, rabbit polyclonal, ChIP gradeFig. 1 Western blotting of HeLa whole cell lysate
20µg of Hela whole cell lysate was run on SDS-PAGE (12.5% gel)anti-HP1γantibody was used at 1µg/ml.
Identification of HP1γ in crude cell extract by Western blotting with this antibody.
Second antibody (goat anti-rabbit IgG antibody, HRP-conjugated, ab205718) was used at 1/10,000dilution.

Fig.2 Immunofluorescence staining of HP1γ protein in HeLa cells with anti- HP1γ antibody.
HeLa cells were fixed with 4%PFA and permeabilized with 0.25% TritonX 100 and reacted with antiHP1γ antibody at 1µg/ml. As the second antibody, anti-rabbit IgG antibody conjugated with Alexa
Fluor 488 (ab150077, Abcam) was used at 1/1,000 dilution. DNA was stained with 1.0μg/mL DAPI inTBS.
References: This product was described and used in Ref 2 and 3
1. Lomberk G et al “The Heterochromatin Protein 1 family” Genome Biol 7: 228 Review (2006) PMID:
17224041
2. Kametaka A et al “Interaction of the chromatin compaction-inducing domain (LR domain) of Ki67 antigen with HP1 proteins” Genes Cells 7: 1231-1242 (2002) PMID: 12485163
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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

3. Wang F et al “The assembly and maintenance of hetero-chromatin initiated by transgene repeats
are independent of the RNA interference pathway in mammalian cells” Mol Cell Biol 26: 40284040 (2006) PMID: 16705157
Related products70-221 Anti-HP1α/CBX5 antibody, rabbit polyclonal70-223 Anti-HP1β/CBX1 antibody, rabbit polyclonal3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/