Anti-Brg1 antibody, rabbit polyclonal, affinity-purified
Product code70-230Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityAffinity-purified with immunogen peptide from rabbit antiserum

ImmunogenC-terminal 50 amino acids of human BRG1, C-KLGRKEKAQDRLK GGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSGSEED, conjugated with KLH

IsotypeRabbit IgG

Reactivity

Human and mouse.

Special notes

N/A

Application

1.

Western blotting (1/1,000 dilution) (Fig. 1)

2.

Immunoprecipitation (Assay dependent)

3.

Immunofluorescence staining (1/1,000 dilution) (Fig. 3)

Not tested in other species

Not tested for other applications.
Background

Data Link

Brg1 (1647 aa, 185 kDa) is transcriptional coactivator cooperating with nuclear hormone
receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex,
a multiprotein complex that regulates promoter activation by orchestrating a calciumdependent release of a repressor complex and a recruitment of an activator complex. In
resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent
recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 isdephosphorylated by calcineurin, which leads to release of the repressor complex. At the sametime, there is increased recruitment of CREBBP to the promoter by a CREST-dependent
mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds
to the NR2B promoter, and activity-dependent induction of NR2B expression involves arelease of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specificchromatin remodeling complex (npBAF complex) and the neuron-specific chromatin
remodeling complex (nBAF complex). During neural development a switch from astem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit
the cell cycle and become committed to their adult state. The transition from proliferatingneural stem/progenitor cells to post-mitotic neurons requires a switch in subunit compositionof the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into
neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, areexchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B orDPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex isessential for the self-renewal/proliferative capacity of the multipotent neural stem cells. ThenBAF complex along with CREST plays a role regulating the activity of genes essential for
dendrite growth. SMARCA4/BAF190A may promote neural stem cell selfrenewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently
making the neural stem cell insensitive to SHH-dependent differentiating cues By similarity.
Also involved in vitamin D-coupled transcription regulation via its association with theWINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR),
which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required forinduction of epithelial-mesenchymal transition (EMT) by ZEB1.

UniProtKB P51532 (SMCA4_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-230 Anti-Brg1 antibody, rabbit polyclonal, affinity-purified

Fig.1 Identification of Brg1 in whole cell extracts of human and mouse cells by western blotting using
anti-Brg1 antibody.
Lane 1; HeLa (human) cell extract
Lane 2; HeLa (human) treated Adriamycin cell extract
Lane 3; NIH 3T3 (mouse) cell extract*Star indicates the position of Brg1 protein bands (Predicted molecular mass of Bgr1 is 185 kDa).
Anti-Brg1 antibody was used at 1/1,000 dilution

Fig 2. Immunoprecipitation of Brg1 protein from nuclear extracts of HeLa cells by using anti-Brg1
antibody.
Brg1 was precipitated with anti-Brg1 antibody and proteinG-conjugated agarose beads and probedwith anti-Brg1 antibody by western blotting.
Lane1; Input nuclear extracts
Lane 2; Control IP with non-immune IgG
Lane 3; Immunoprecipitation with anti-Brg1 antibody
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Anti-Brg1 antibody

DAPIMerge

Fig.3. Immunofluorescence staining of Brg1 protein in HeLa cells.
Hela cells were fixed in 4% paraformaldehyde overnight and permeabilized
in 0.25% TritonX 100 in PBS for 10 min. Anti-Brg1 antibody was used at 1/1,000 dilution.
As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/1,000 dilution. Nuclei
were stained with DAPI. Brg1 protein is localized in nuclei.

Fig 4．Silencing of Brg1 expression by Brg1-specific siRNA
HeLa cells were transfected with the siRNA described in the paper. 48 hr later, Brg1 in total cell extracts wasdetected by Western blotting. The signal intensity of the blot was scanned and displayed as 100% of control sample.

Publication: This antibody has been described and used in the following publication.
Nishimoto N. et al (2012) Heterocomplex formation by Arp4 and β-actin is involved in the integrity of
the Brg1 chromatin remodeling complex. J Cell Sci.125: 3870-82. PubMed WB, IP

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