Anti-NAI2 ΔSP (At) antibody, rabbit polyclonal
Product code81-103Size

200 µgStorage-20℃

Concentration

2.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenPurified recombinant His6-tagged NAI2 (amino acids 272-502) protein lacking
signal peptide (A. thaliana).

IsotypeRabbit IgG

Reactivity

NAI2 protein of Arabidopsis thaliana. Not tested in other species.

Special notes

Validation: Specific reactivity has been validated by western blot using nai1
mutant extracts.

Application1. Western blotting (1/2,000-1/4,000)
2. Immunofluorescence staining (1/1,000-1/3,000)

BackgroundResponsible for the ER body formation. Regulates the number and shape of theER bodies and the accumulation of PYK10 in ER bodies, but is not involved in
the expression of PYK10. Interacts directly or indirectly with MEB1 and MEB2.
Expressed in roots. Detected in shoot apex. Induced by NAI1.Lenght:772: aminoacids.
Predicted molecular mass: 85,016
Subcellular location: Endoplasmic reticulum lumen.
Modification: Elimination of 24-amino acid signal peptide from N-terminus.

Data Link

UniProtKB Q9LSB4(NAI2_ARATH)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 81-103 Anti-NAI2 ΔSP (At) antibody, rabbit polyclonal

Fig.1 Western Blot of NAI2 in extract of arabidopsis.seedling
Crude extract of 7day old seedling of Arabidopsis thaliana was run on 12.5% SDS-PAGE and blotted
overnight to PVDF membrane by wet system. Anti-MEB1 antibody was used at 1/4,000 dilution.
Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000dilution.
Among the two bands the lower band was suggested to be degradation product of the upper band(Ref.1)

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Fig.2 Validation of the anti-NAI2 (ΔSP)the antibody specificity by western blot using extracts ofmutant seedling of Arabidopsis.
Total proteins were extracted from 20 plants of 7-d-old seedlings using 200 ml of 23 sample buffer (20mM Tris-HCl buffer, pH 6.8, 40% glycerol, 2% SDS, and 2% 2-mercaptoethanol). The extracts (10 ml)
were subjected to SDS-PAGE (12.5% acrylamide gel). The separated proteins were transferred to anylon membrane and subjected to western blot analysis using anti-NAI2(ΔSP) antibody at 1/2,000
dilution.
As the second antibody, goat anti-rabbit IgG antibody HRP-conjugated was used at 1/10,000 dilution.
Samples. 1. Wild-typ. 2 Wild-type with GFP-h 3.nai2-1. 4. nai2-2. 5. nai2-3 6.nai1-1

Fig.3 Immunofluorescence staining of NAI peotein in ER bodies.
Immunofluorescence analysis of NAI2 in 7-d-old GFP-h seedlings. Left panels, the ER-targeted GFPsignals; middle panels, the NAI2 signals, which were detected by antibodies against NAI2/ΔSP, andCy3-labeled second antibodies; right panels, the merged images. Bars = 10 mm.

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Reference: This antibodyhas been described I Ref.1 and used in the following publications.
1. Yamada K et al. NAI2 is an endoplasmic reticulum body component that enables ER bodyformation in Arabidopsis thaliana. Plant Cell. 2008 Sep;20(9):2529-40. PMID: 18780803 WB, IF(Arabidopsis)
2. Yamada K et al. Identification of two novel endoplasmic reticulum body-specific integral
membrane proteins. Plant Physiol. 2013 Jan;161(1):108-20. PMID: 23166355 WB (Arabidopsis)
3. Ueda H et al. Endoplasmic Reticulum (ER) Membrane Proteins (LUNAPARKs) are Required for
Proper Configuration of the Cortical ER Network in Plant Cells. Plant Cell Physiol. 2018 Oct
1;59(10):1931-1941. PMID: 30010972 WB (Arabidopsis)
Related Products81-101 Anti-MEB1 (At) antibody, rabbit polyclonal
81-102 Anti-MEB2 (At) antibody, rabbit polyclonal
81-104 Anti-NAI2 C-terminal (At) antibody, rabbit polyclonal

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