Anti-Fd-GOGAT (Ferredoxin-dependent glutamate synthase, chloroplastic) (Maise)
antibody, rabbit polyclonal
Product code81-025Size

100 µgStorage-20℃

Concentration

2.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenPurified recombinant maize Fd-GOGAT protein, full-size, no-tag attached

IsotypeRabbit IgG

Reactivity

Fd-GOGAT including those of maize, arabidopsis, spinach, and cyanobacterium(Synechococcus)

Special notes

N/AApplication

1. Western blotting (1/2,000-1/5,000 dilution)
2. ELISA (assay dependent)
Other applications have not been tested.

Background

Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an
enzyme involved in synthesis of glutamate from glutamine and alphaketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT(Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT
found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1
and glu2 in Arabidopsis. Fd-GOGAT (both forms) is highly conserved amongplants, red algae, and cyanobacteria. Ferredoxin-dependent glutamate
synthase, chloroplastic (Fd-GOGAT) is involved in glutamate biosynthesis inleaf. This protein required for the reassimilation of ammonium ions generated
during photorespiration. Gene name is GlsF.

Data Link

UniProtKB: P23225 (Z. mays), Q51579 (P. boryanum), P55038 (Synechocystissp.)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 81-025 Anti-Fd-GOGAT (Ferredoxin-dependent glutamate synthase, chloroplastic)(Maise) antibody, rabbit polyclonal

Fig.1 Western Blot of Fd-GOGAT in plant leaf extract.
1. Full-size recombinant maize Fd-GOGAT protein
2. Arabidopsis leaf extract, 10 μg
3. Maize leaf extract, 10 μg
Anti-Fd-GOGAT antibody was used at 1/2,500 dilution. Second antibody (goat anti-rabbit IgG
antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
Molecular masses of maize and arabidopsis Fd-GOGAT are 175 kDa and 168 kDa, respectively.

Fig.2 Western Blot of Fd-GOGAT in cyanobacterium
Sample: Crude extract of Synechococcus spp.6803
The Fd-GOGAT antibody was used at 1/2000 dilutions
Molecular mass is 169 kDa
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Reference: This antibody has been used in the following publications.
1. Sakakibara H. et al. Molecular cloning and characterization of complementary DNA encoding for
ferredoxin-dependent glutamate synthase in maize leaf. J Biol Chem. 1991 Feb 5;266(4):2028-35.
PMID: 1989968. WB; maize
2. Sakakibara H., Kawabata S., Hase T. and Sugiyama T. (1992) Differential effects of nitrate andlight on the expression of glutamine synthetase andferredoxin-dependent glutamate synthase inmaize. Plant Cell Physiol., 33, 1193-1198. Googl Scholar:abstract/33/8/1193/1860644

WB; maize

3.Kimata-Ariga Y and Hase T. Multiple complexes of nitrogen assimilatory enzymes in spinach
chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One. 2014 Oct
1;9(10):e108965. PMID: 25271437 WB;spinach

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