Anti-Rhp51 (S. pombe) antibody, rabbit serum
Product code

63-001Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.09% sodium azide

PurityRabbit antiserum

ImmunogenPurified recombinant full-length Rhp51 protein

IsotypeRabbit IgG

Reactivity

Schizosaccharomyces pombeSpecial notes

Validation: Specificity has been validated by western blotting with rhp51
deletion mutant (Fig.1)

Application

1. Western blotting (1/ 2,000~1/5,000 ) Fig.1
2. Immunoprecipitation (1/100-1/500)
3. Chromatin Immuno-Precipitation (Assay dependent)
4. Immunofluorescence staining (1/500 dilution). Fig. 2

BackgroundRhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional andstructural homolog of E.coli RecA protein and Rad51 proteins of eukaryotes,
which play a major role in genetic recombination and recombination repair bymediating strand exchange reaction between homologous DNA strands.

Data Link

UniProtKB/Swiss-Prot P36601 (RAD51_SCHPO)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 63-001 Anti-Rhp51 (S. pombe) antibody, rabbit serum

Fig.1 Western blot analysis of Rhp51 in the whole cell extracts.
M: Molecular size markers (kD)
Lane 1: Wild-type strain
Lane2: Rhp51 deletion mutant strain

Fig.2 Rhp51 foci formation observed after DNA damage
S. pombe cells without or with MMS (0.025%) treatment for 1 h were processed for indirect
immunofluorescence staining with anti-Rhp51 antibody (1/500 dilution).

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Reference: This product has been used in the following publications
1. Akamatsu Y et al. Two different Swi5-containing protein complexes are involved in mating-type
switching and recombination repair in fission yeast. Proc Natl Acad Sci U S A. 2003 Dec
23;100(26):15770-5. WB, IP (S. pombe)
2. Kibe T et al. Fission yeast Rhp51 is required for the maintenance of telomere structure in theabsence of the Ku heterodimer. Nucleic Acids Res. 2003 Sep 1;31(17):5054-63. ChIP (S. pombe)
3. Lambert S et al “Gross chromosomal rearrangements and elevated recombination at an inducible
site-specific replication fork barrier” Cell 121: 689-702 (2005) PMID: 15935756 IF (S. pombe)
4. Morishita T et al ” Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing
recombination intermediates” Mol Cell Biol 25: 8074-8083 (2005) PMID: 16135799 IF (S.pombe)
5. Haruta N et al "The Swi5-Sfr1 complex stimulates Rhp51/Rad51-and Dmc1-mediated DNA strand
exchange in vitro”Nat Struc Mol Biol 13: 823-830 (2006) PMID: 16921379
WB, IP (S. pombe)
6. Akamatsu Y et al. Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51dependent recombination outcomes. EMBO J. 2007 Mar 7;26(5):1352-62. IF (S. pombe)
7. Polakova S et al. Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper
Meiotic Chromosome Segregation. PLoS Genet. 2016 Jun 15;12(6):e1006102. IF (S. pombe)
8. Yadav RK. Histone H3G34R mutation causes replication stress, homologous recombination
defects and genomic instability in S. pombe. Elife. 2017 Jul 18;6. pii: e27406. PMID: 28718400.
WB, IF (S. pombe)

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/