Anti-Varicella Zoster Virus (VZV) gH antibody, mouse monoclonal (62B)
Product code65-353Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Varicella-zoster virus Oka strain (vaccine strain)

Isotype

Mouse IgG1κ

Reactivity

IE62 of VZVSpecial notes

N/A

Application

1. Western blotting (1/2,000-1/5,000)
2. Immunoprecipitation (1/100)
3. Imunofluorescence staining and Immunocytochemistry (1/50-1/100)
Not tested for other applications

Background

Varicella Zoster Virus (VZV) is one of eight herpesviruses known to infect humans
and vertebrates. VZV only affects humans, and commonly causes chickenpox inchildren, teens and young adults and herpes zoster (shingles) in adults and rarely
in children. VZV is known by many names, including chickenpox virus, varicellavirus, zoster virus, and human herpesvirus type 3 (HHV-3).
VZV infects the nerves and causes a wide variety of symptoms. After the primary
infection (chickenpox), the virus goes dormant in the nerves, including the cranial
nerve ganglia, dorsal root ganglia, and autonomic ganglia. Many years after thepatient has recovered from chickenpox, VZV can reactivate to cause a number ofneurologic conditions.
Immediate early protein 62 (IE62) of is major transcriptional transactivator of VZV.
It may interact with and recruit specific components of the general transcription
machinery to viral promoters and stabilize their formation for transcription
initiation. It interacts with IE4 and IE63, and with host USF and Sp1.It negatively
regulates its own transcription. This immediate early protein may be necessary invirion for viral pathogenesis. IE62 consists of 1,310 amino acids with 140 kDa mass.
It is phosphorylated by ORF66 protein kinase on Ser-686 and Ser-722. It is also
phosphorylated by ORF47 protein kinase and human CSNK2A1/CKII.SK

Data Link

UniProt P09310 (ICP4_VZVD).

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 65-353 Anti-Varicella Zoster Virus (VZV) IE62 antibody, mouse monoclonal (62B)

Fig.1. Identification of IE62 protein in VZV-infected cells by western blotting using anti-VZV IE62
antibody (clone 62B).
Lane 1; VZV strain pOka infected MRC-5 cell lysate
Lane 2; MRC-5 cell lysate ( uninfected negative control )The anti-VZV IE62 antibody was used at 1/5,000 dilution.

Fig.2. Immunofluorescence staining of VZV IE62 protein in VZV-infected MRC-5 cells
VZV-infected MRC-5 cells were collected at 3 days postinfection. The cells were fixed in cold acetone
and incubated at 37 °C for 1 h with anti-VZV IE62 antibody at 1/100 dilution. After washing withPBS for 10 min, Alexa Fluor 488 donkey anti-mouse IgG [H+L] (Life Technology No. A21202) wasused at 1/200 dilution as the second antibody.
Nuclei were stained with Hoechst 33342. Signals were detected by confocal microscopy.
2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Reference: This antibody has not been cited in publication.
Related Product65-350 Anti-Varicella Zoster Virus (VZV) IE62 antibody, mouse monoclonal (62A)

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/