For Research Use Only.
Not for use in diagnostic procedures.

566-H
Lot 020~
Page 1POLYCLONAL ANTIBODY

Anti-CD117 (c-Kit) (Human) pAb
Code No.
566-H

Quantity
6 mL

BACKGROUND: c-Kit, also known as stem cell factor

receptor, steel factor receptor or CD117 is classified as a
type III receptor tyrosine kinase (RTK) belonging to theplatelet-derived growth factor receptor subfamily. Bindingof stem cell factor (SCF), known as c-Kit ligand to c-Kit,
initiates autophosphorylation of the receptor, subsequently
leading to promotes a signal transduction cascade throughRas-Raf-MAP kinase cascade, phosphatidylinositol3-kinase, src family kinases, and JAK/STAT pathways. Theroles of c-Kit include maturation of hematopoietic andprimordial germ cells precursors and melanocytes during
embryonic development. In acute myeloid leukemia (AML),
c-Kit has been proposed to play a functional role, andbecomes target molecule for drug development.

Form
Ready for use

SPECIES CROSS REACTIVITY:
Species

Human

Mouse

Rat

Cell and Tissue

HEL,pancreas

Not tested

Not tested

Reactivity on IHC+INTENDED USE:

For Research Use Only. Not for use in diagnostic procedures.

SOURCE: This antibody was purified from rabbit serum

using the synthesized peptides (C-terminal of c-Kit geneproduct; K963) coupled protein A agarose column. The
rabbit was immunized with carrier protein conjugatedsynthesized peptides (K963).

FORMULATION: 6 mL volume of pre-diluted

antibody in 20 mM HEPES (pH 7.2), containing 135 mM
NaCl, 1% BSA and 0.09% NaN3.
*Azide may react with copper or lead in plumbing system to form
explosive metal azides. Therefore, always flush plenty of water
when disposing materials containing azide into drain.

STORAGE: This antibody solution is stable for 3 years
from the date of purchase when stored at 4°C.

REACTIVITY: This antibody reacts with c-Kit on
Immunohistochemistry.

APPLICATIONS:

Immunohistochemistry; Ready for use
Heat treatment is necessary for paraffin embedded
sections.
Microwave oven; 2 times for 10 minutes each in 10 mM
citrate buffer (pH 6.5)

*Please refer to the data sheet (MBL code no. 566) for otherapplications.
Detailed

procedure

PROTOCOL.

isprovidedinthefollowing

URL https://ruo.mbl.co.jp e-mail support@mbl.co.jp

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REFERENCES:

1) Koch, A. C., et al., Ann. N.Y. Acad. Sci. 1073, 517-526 (2006)
2) Lyford, G. L., et al., Gut 51, 496-501 (2002)
3) Sakurai, S., et al., Am. J. Pathol. 154, 23-28 (1999)
4) Tsuura, T., et al., Virchows Archiv. 424, 135-141 (1994)
5) Hidi, K., et al., Oncogene 6, 2291-2296 (1991)
6) Yarden,Y., et al., EMBO J. 6, 3341-3351 (1987)

RELATED PRODUCTS:

Please visit our website at https://ruo.mbl.co.jp/.

This antibody is used in the reference number 1) - 3).
The descriptions of the following protocols are examples.
Each user should determine the appropriate condition.

PROTOCOL:

Immunohistochemical staining for paraffin-embedded
sections

1) Deparaffinize the sections with Xylene 3 times for 3-5
minutes each.
2) Wash the slides with Ethanol 3 times for 3-5 minuteseach.
3) Wash the slides with PBS 3 times for 3-5 minutes each.
4) Heat treatment
Heat treatment by microwave oven:
Place the slides put on staining basket in 500 mL beakerwith 500 mL citrate buffer (pH 6.5). Cover the beakerwith plastic wrap, then process the slides 2 times for 10minutes each at 500 W with microwave oven. Let theslides cool down in the beaker at room temperature forabout 40 minutes.
5) Remove the slides from the citrate buffer and cover eachsection with 3% H2O2 for 10 minutes at room temperature
to block endogenous peroxidase activity. Wash 3 times in
PBS for 5 minutes each.
6) Remove the slides from PBS, wipe gently around eachsection and cover tissues with 1% BSA in PBS for 5minutes to block non-specific staining. Do not wash.
7) Tip off the blocking buffer, wipe gently around eachsection and cover tissues with one or two drops ofprimary antibody (Ready for use).
8) Incubate the sections for 1 hour at room temperature.
9) Wash the slides 3 times in PBS for 5 minutes each.
10) Wipe gently around the section and incubate withHistostar™ (Rb) (MBL; code no. 8466) for 30 min. atroom temperature. Wash as in step 9).
11) Visualize by reacting for 5 min. with Histostar™ DAB
Substrate Solution (MBL; code no. 8469). *DAB is a suspect
carcinogen and must be handled with care. Always wear gloves.

12) Wash the slides in water for 5 minutes.
13) Counter stain in hematoxylin for 1 minute, wash the slides
3 times in water for 5 minutes each, and then immerse theslides in PBS for 5 minutes. Dehydrate by immersing in
Ethanol 3 times for 3 minutes each, followed byimmersing in Xylene 3 times for 3 minutes each.
14) Now ready for mounting.
(Positive controls for immunohistochemistry; HEL andhuman pancreas)

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