Anti-EDD/UBR5 antibody, rabbit polyclonal
Product code70-501

Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityAffinity-purified with immunogen.

ImmunogenSynthetic peptide of human EDD protein corresponding to 394-408 amino acids,
C-KWSESEPYRNAQNPS, conjugated with KLH

IsotypeRabbit IgG

Reactivity

Human (HeLa, HEK293T, MCF7) and mouse (NIH3T3).

Note that expression

level of EDD varies greatly, depending on tissues and cell types (see Fig.1).
Special notes

N/A

Application

1. Western blotting (1/1,000)
2. Immunofluorescent staining 1/1,000)

Background

EDD / UBR5 (2,799 aa, 309 kDa) is E3 ubiquitin-protein ligase which is acomponent of the N-end rule pathway. Recognizes and binds to proteins bearing
specific N-terminal residues that are destabilizing according to the N-end rule,leading to their ubiquitination and subsequent degradation By similarity.
Involved in maturation and/or transcriptional regulation of mRNA by activating
CDK9 by polyubiquitination. May play a role in control of cell cycle progression.
May have tumor suppressor function. Regulates DNA topoisomerase II binding
protein (TopBP1) in the DNA damage response. Plays an essential role inextraembryonic development. Ubiquitinates acetylated PCK1. Also acts as aregulator of DNA damage response by acting as a suppressor of RNF168, an E3
ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone
H2A and H2AX at DNA damage sites, thereby acting as a guard against
excessive spreading of ubiquitinated chromatin at damaged chromosomes.

Data Link

uniprot/O95071 UBR5 humanPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-501 Anti-EDD/UBR5 antibody, rabbit polyclonal

Fig.1 Identification of EDD in whole cell extracts of human cells by western blotting using anti-EDD
antibody.
Lane 1; HeLa cells transfected with control siRNA
Lane 2; HeLa cells transfected with EDD-specific siRNA
Lane 3; HEK293T cells transfected with pFlag-CMV-5b empty vector
Lane 4; HEK293T cells transfected with pCMV-Tag2b-EDD expression vector
Predicted molecular mass of EDD is 309 kDa and the corresponding band in Lane 1 is much reducedin Lane 2 by introduction of the EDD-specific siRNA. Note that expression level of EDD in HEK293T
cells is much higher than in HeLa cells. Mol Mass of EDD is 309 kDa

Fig.2. Immunofluorescence staining of EDD protein in MCF7 cells.
MCF7 cells were fixed in 4% paraformaldehyde overnight and permeabilizedin 0.25% TritonX 100 in PBS for 10 min. Anti-EDD antibody was used at 1/1,000 dilution.
As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/1,000 dilution. DNAwas stained with DAPI.Note that not all nuclei were stained, indicating cell cycle dependency of EDD
protein existence.
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BioAcademia,Inc. Tel. 81-6-6877-2335

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Reference: This antibody has been used in the following publications.
1. Watanabe S et al. GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasomesystem. J Cell Sci. 2018 Aug 3;131(15). PMID:29991511 WB (human)

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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/