BIOFLOAT™
The anti-adhesive surface
for spheroid culture

2

Advantages ofspheroid culture
Increased cell-cell contacts
Pronounced extracellular matrix
Improved in vitro model
In many areas of biomedical research,in vitro models are indispensable.
The most conventional form is thetwo-dimensional cell culture, but
discrepancies often occur when transferring the results to an entire organism.
The aim of the three-dimensional cell
culture is therefore to close this gap
between the in vitro and in vivo situation.

Spheroid cultures offer a simple andcost-effective variant of 3D cell culture.
The cells form a three-dimensional
cellular aggregate with pronounced
cell-cell and cell-matrix contacts.
The new BIOFLOAT™ cell culture surface
makes it possible to produce perfectspheroids quickly and reproducibly.

BIOFLOAT™ is used in a variety of fields
such as cancer and stem cell research,in the preclinical phase of drug researchand in toxicological studies. Spheroid
cultures improve the efficiency andreliability of preclinical cell models inthese applications.

BIOFLOAT™

750 cells/welluncoated

1,500 cells/well

3,000 cells/well6,000 cells/well6,000 cells/wellFig. 1: Cells of a fibroblast cell line (3T3) were seeded in various cell counts on the BIOFLOAT™ cell culture plate. An uncoatedplate serves as a control. The results were documented microscopically after three days. It can be clearly seen that spheroids
are successfully formed using BIOFLOAT™. In addition, the size of the spheroid can be influenced by the number of cells/well.
On the uncoated surface however, the fibroblasts can adhere and do not form spheroids.

BIOFLOAT™  ///  SARSTEDT 3

BIOFLOAT™ solvesyour spheroid culturechallenges

Some challenging spheroid cultures havealready been successfully establishedusing the BIOFLOAT™ cell culture
surface (e.g. spheroids from primaryhepatocytes). A list of cell lines and cell
types successfully tested with
BIOFLOAT™ can be found on page 6.

Try it out for free andwithout obligation!

Brain cells
Dental cells

shop.sarstedt.us/biofloat

Lung cells
Cardiac muscle cells

Liver cells
Epithelial cells

Pancreatic cells

Bone cells

Kidney cells

Endothelial cells

Stromal cells

4

Why BIOFLOAT™?
Robust coating, defined composition,easy handling, fast results, high reproducibility

The polymer coating of the BIOFLOAT™
surface modifies the plastic surface in asimple way. The inert coating contains
molecules that are anchored to thepolystyrene surface by strong physicalinteractions and self-organizing capability. This achieves a particularly uniformtreatment.

The robustness of the BIOFLOAT™
coating greatly facilitates daily work.
The performance of the BIOFLOAT™ cell
culture surface is not impaired even bymultiple washing steps or mechanicalaction from a pipette tip (see Fig. 2).

BIOFLOAT™

Spheroids cultivated using the BIOFLOAT™ surface have a very even roundshape. Usually, the formation of exactly
one spheroid per well is achieved. Bothof these features lead to a high reproducibility of results. BIOFLOAT™ is thus
particularly suitable for high throughput

analyses where it is especially important
to examine exactly one symmetrical
spheroid per well.

Control

Lightly scratched

Heavily scratched

Competitor A

The BIOFLOAT™ surface features ahighly anti-adhesive property. This
makes it possible for the cultivatedadherent cells to form primarily cell-cell
contacts without adhering to the surfaceof the vessel.

Control

Lightly scratched

Heavily scratchedFig. 2: The well bottom was lightly scratched using a standard pipette tip (once all around with moderate pressure) and heavily
scratched (30 s with strong pressure). 200 μl of a suspension of 3T3 cells with a concentration of 30,000 cells/ml (corresponding to 6,000 cells/well) was then seeded per well.

BIOFLOAT™  ///  SARSTEDT 580604020

BIOFLOAT™

Competitor A

12011296Time (h)10488807256644832402408Fig. 3: 200 μl of a suspension of 3T3 cells with a concentrationof 30,000 cells/ml (corresponding to 6,000 cells/well) was
seeded per well. Wells with exactly one spheroid were identified and plotted in percent as a function of incubation time.

10016The BIOFLOAT™ surface enables rapid spheroid formation.
Depending on the cell line or cell type, the formation of thespheroids on the BIOFLOAT™ surface takes between 2 and 24hours. Uniform spheroids have been shown to form more
rapidly than on most anti-adhesive, cell-repellent surfaces(Fig. 3).

1200Fast spheroid formation

Portion of wells with single spheroid (%)

BIOFLOAT™ enables spheroid cultures –quickly, uniformly and reliably

Competitor B

High reproducibility
0.65
0.60
0.55
0.50
0.45

BIOFLOAT™

The reliable quality of the BIOFLOAT™ cell culture surface
enables the formation of perfect spheroids even for challenging cells. This also includes cells that do not form spheroids
on existing products.

Competitor A

BIOFLOAT™

Day 0Day 2Competitor ADay 4120112

1049688807256644840322416Time (h)Reliable spheroid culture

Fig. 5: 100 μl of a suspension of primary human hepatocyteswith a concentration of 25,000 cells/ml (equivalent to 2,500cells/well) was seeded per well. After spheroid formation,
50 μl of medium was exchanged every 48-72 h.

80.40
0Fig. 4: 200 μl of a suspension of 3T3 cells with a concentrationof 30,000 cells/ml (corresponding to 6,000 cells/well) was
seeded per well. The relative circularity of the spheroids
formed was determined and plotted as a function of time. Thehigher the value, the rounder the spheroid. A value of 1 would
correspond to a perfect circle.

0.70

Relative circularity

Spheroids formed with the help of the BIOFLOAT™ surface
have a high circularity, which enables a high data consistency(Fig. 4). No deposits, satellite aggregates or irregular aggregates are formed, which ensures a high reproducibility.

Competitor B

Day 8

6

BIOFLOAT™ leads tosuccessful and reliable
spheroid formation evenwith challenging cells

The following cells have already been successfully tested for
the BIOFLOAT™-mediated spheroid culture.

NameDescription

3T3Fibroblasten (M. musculus)

A431

Squamous cell cancer cell line (H. sapiens)

B16

Melanoma cell line (M. musculus)

CaCo-2

Colon carcinoma cell line (H. sapiens,kaukasisch)Capan-1

Name

Description

huARLT

Immortalised endothelial cells (from HUVECcells) (H. sapiens)

HuOB

Immortalised osteoblasts (H. sapiens)

huVEC

Venous endothelial cells (H. sapiens)

iPSC-Gata6

iPSC-derived hepatocytes

Pancreatic adenoma cancer cell line (H. sapiens)

MCF10A

Breast cancer cell line (H. sapiens)

CHO

Ovarian cell line (C. griseus)

MCF-7

Breast cancer cell line (H. sapiens)

D492

Epithelial breast cancer cell line(stem cell-like) (H. sapiens)

MDA-MB231

Breast cancer cell line (H. sapiens)

Tumourigenic breast epithelial stem cell linefrom D492 cells (H. sapiens)

Mia-Paca

Pancreatic cell line (H. sapiens)

D492HER

Panc1

Pancreatic cell line (H. sapiens)

DAN-G

Pancreatic cancer cell line (H. sapiens)

Panc39Pancreatic cell line (H. sapiens)

ESCs

Embryonic stem cells (S. scrofadomesticus)

PRH with RHSteC

Hepatic stellate cells/Ito cells(R. norwegicus)

FAMPAC

Pancreatic adenoma cancer cell line (H. sapiens)

PRH+ HHSteC

Hepatic stellate cells/Ito cells (H. sapiens)

H1975

Lung adenocarcinoma cell line (H. sapiens)

RPMI

H2228

Lung adenocarcinoma cell line (H. sapiens)

B-lymphocyte cell line from myeloma patients(H. sapiens)

H3122

Lung adenocarcinoma cell line (H. sapiens)

SFFV2

Immortalised astrocytes (H. sapiens)

HCC1433

Breast cancer cell line (H. sapiens)–Differentiated fatty cell organoids frompluripotent stem cells

HCT-116

Colon cancer cell line (H. sapiens)

–

hDPSC

Primary dental pulp stem cells (H. sapiens)

Endometrial organoids from detached primarycells (non-human primates)

hDPSC+Panc1

Pancreatic cancer cell line (H. sapiens)–

Fibroblast progenitor cells (M. cerebralis)

HEK293

Embryonic kidney cells (H. sapiens)–

iPSC-derived cardiomyocytes (H. sapiens)

HepG2

Hepatoma cell line (H. sapiens)–

Liver organoids (differentiated) (M. musculus)

Colon adenocarcinoma cell line (H. sapiens,Caucasian)–

Neuronal stem cells (HN9 differenziert)

HT-29

–

Primary hepatocytes (H. sapiens, M. musculus,
M. fascicularis, C. lupus familiaris)

BIOFLOAT™  ///  SARSTEDT 7

Test the BIOFLOAT™ cell
culture plates in yourprocess environment—
order free samples here:
biofloat.sarstedt.com

The SARSTEDT BIOFLOAT™ plate is individually sterilepacked in an aluminum bag. It is also endotoxin-free andnon-cytotoxic.

Order information
Order no.

Name

Number of wells

83.3925.400

Cell culture plate, 96-well, surface:
BIOFLOAT™, round bottom

96

BIOFLOAT™ – atechnology

Bottom shapePackaging
1 pc./aluminium bag
4 Pcs./Inner carton
24 Pcs./Outer carton

If you have any questions:We‘d be happy to help!

SARSTEDT, Inc.
1025 St. James Church Road
P.O. Box 468
Newton, NC 28658 - 0468
Toll free: +1 800 257 5101
customerservice.us@sarstedt.com
www.sarstedt.com

BRO_25_915_0200_501_NF   This document may contain information on products that may not be available in particular countries   Technical modifications reserved

Visit our website:www.sarstedt.com