anti-hnRNP-U / SAF-A antibody, rabbit serum
Product code70-415

Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityRabbit antiserum

Immunogen

Recombinant MBT-fused mouse hnRNP-U (aa 614-800)

IsotypeRabbit IgG

Reactivity

Reacts with mouse and rat, and predicted to react with human from the aminoacid sequence homology.

Special notes

N/AApplication

1. Western blotting (dilution: 1/3,000-1/1,000)
2. Immunocytochemistry (dilution: 1/1,000-1/500)
3. Immunoprecipitation

Background

Heterogeneous nuclear ribonucleoprotein U (hnRNP-U, also known as scaffold
attachment factor A, SAF-A) is a nuclear matrix-associated protein thatinteracts

withchromosomal

DNA.

hnRNP-U

specificallybinds

toscaffold/matrix attachment region of DNA and could thus be involved in higher
order chromatin structure. hnRNP-U is also a RNA binding protein and forms
complexes with heterogeneous nuclear RNA (hnRNA) and plays an importantrole in pre-mRNA processing and transport.
HnRNP-U is reported to interact with necdin, a growth suppressor that isexpressed in terminally differentiated neurons and skeletal muscle cells. It has
been shown that hnRNP-U recruits necdin to the nuclear matrix where theyform a stable complex. It is suggested that necdin suppresses cell proliferation
through its interaction with hnRNP-U in the specific subnuclear structure(ref.2).

Data Link

Q8VEK3 (mouse), Q00839 (human)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 70-415 anti-hnRNP-U / SAF-A antibody, rabbit serum

Fig.1 Immunoblotting of hnRNP-U with this antibody (ref.2).
Specificity of anti-hnRNP-U antibody, HUT.
Cell lysates were prepared from SAOS-2 cells transfected with pRc/CMV vectors (pRc) or pRc/CMV
vectors expressing Myc-tagged hnRNP-U (Myc-UF). Exogenous Myc-tagged hnRNP-U (Myc-U) andendogenous hnRNP-U (U) proteins were detected by immunoblotting with anti-Myc antibody (αMyc)
or HUT (αU).
This antibody recognized exogenous Myc-tagged hnRNP-U and endogenous ~120 kDa hnRNP-U
proteins in SAOS-2 cells.

Fig.2 Immunocytochemistry using this antibody, HUT (ref.2)
Mouse P19 neurons were labeled with anti-necdin antibody (Ndn) (a) or with HUT for hnRNP-U (U)(c) in combination with anti-neuronal marker, MAP2, antibody for MAP2 (b, d). The nuclear matrix
was prepared in situ and labeled for necdin (Ndn) (e), hnRNP-U (U) (f), and a nuclear matrix marker,
lamin B (g).

2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/Both necdin and hnRNP-U were localized to the nuclei of differentiated neurons, which express theneuronal marker MAP2 (a-d). Necdin was also distributed in the neuronal cytoplasm (a).
The immunocytochemical analysis of in situ extracted nuclear matrix revealed that both necdin andhnRNP-U were concentrated in intranuclear speckles throughout the nucleoplasm (e, f). Lamin B, anuclear matrix marker, was localized to the nuclear lamina (g). These results suggest that both necdin
and hnRNP-U are associated with the nuclear matrix of neurons.

References: This antibody was produced and used in ref.2.
1. Kiledjian M and Dreyfuss G (1992) “Primary structure and binding activity of the hnRNP U
protein: binding RNA through RGG box.” EMBO J 11: 2655-2664 PMID: 1628625
2. Taniura H and Yoshikawa K (2002) “Necdin interacts with the ribonucleoprotein hnRNP U in thenuclear matrix.” J Cell Biochem 84:545-555 PMID: 11813259
Related product:74-104 anti-APP (C-terminal) antibody, rabbit serum

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/