The eDNA
Society International
Meeting 2023
Moving from knowledge into practice

Excursions

20 21

SUN

WED

17 19
SAT

Core
dates

May
2023

Venue

Piazza Omi, Otsu, Japan
Chair

Toshifumi Minamoto

Prof. Dr.
Kobe University

FRI

ProgramMay
2023

Program
Meeting schedule (UTC+9)
Day-1 May 17th (Wed)14:00 - 15:00

Opening Ceremony
ANEMONE: Japan’ s eDNA-based biodiversity monitoring network
Michio Kondoh, Ph.D., Prof. (Tohoku University, Japan)

15:30 - 17:30

Plenary Session 1
From innovation to practice: Using eDNA to support evidence-based management
Hiroki Yamanaka, Ph.D., Associate Prof. (Ryukoku University, Japan)

18:00 - 19:30

BanquetDay-2 May 18th (Thu)
09:00 - 11:00

Plenary Session 2
Understanding biodiversity hotspots using eDNA
Hitoshi Araki, Ph.D., Prof. (Hokkaido University, Japan)
Tadashi Kajita, Ph.D., Prof. (University of Ryukyus, Japan)

11:00 - 12:30

Corporate Workshop 1
Illumina K.K.

13:00 - 14:15

Oral Presentation

14:30 - 17:00

Poster Presentation (14:30 - 16:30 Core Time)

17:15 - 18:30

Oral Presentation

Day-3 May 19th (Fri)
09:00 - 11:00

Plenary Session 3
Environmental DNA moving from knowledge into practice
Hideyuki Doi, Ph.D., Prof. (Kyoto University, Japan)

11:00 - 12:30

Corporate Workshop 2
Go!Foton, Inc. and Pacific Consultants Co., LTD.

13:00 - 15:45

Oral Presentation

16:00 - 17:00

Closing Ceremony
Is eDNA good enough? Implications for science and society
Mehrdad Hajibabaei, Ph.D., Associate Prof. (University of Guelph, Canada)

1

Message

It is a great honor for us to host the first international conference of the eDNA Society. “The eDNA SocietyInternational Meeting 2023” will be held at Otsu City, Shiga Prefecture, on the shores of Lake Biwa, the largest
and oldest lake in Japan. Lake Biwa is home to many endemic organisms and is the birthplace of eDNA studies
in Japan, and research has been conducted on a wide range of organisms, from microbes to higher animals,present in the lake.
eDNA research began in Japan around 2009, and targeted Lake Biwa and its surrounding areas. In 2018, theeDNA Society was established with the participation of many members. Apart from Japan, eDNA research isextensively being conducted in Europe, North America, Oceania, and several other regions. The first eDNA
study on a macroorganism was published 15 years ago in 2008. Presently, eDNA studies are transforming from amere discipline of natural science into an important research tool for conserving global biodiversity. Under thetheme of "Moving from knowledge into practice," the purpose of this conference is to provide a forum fordiscussing the practical applications of the knowledge we have gathered over the years. Biodiversity loss isoccurring simultaneously throughout the planet. Therefore, it is important for eDNA researchers from around theworld to gather together and discuss common issues such as accurate monitoring of biological distributions overlarge areas, establishment of protected areas based on the identification of breeding grounds, and, of course, toshare the excitement of the science.
Otsu City, the host city, is adjacent to Kyoto, the ancient capital of Japan. Participants will thus be able toexperience not only the rich natural environment of Lake Biwa, but also the history and culture of Japan. We
look forward to welcoming you to Japan.
The chair of The eDNA Society International Meeting 2023
Toshifumi Minamoto, Ph.D., Prof.

2

Map & Direction (Piazza Omi)
Kansai Airport
Limited Express Train (*1)
KansaiInternational Airport (KIX)

Approx. 1h 20mins.
JR Biwako line

On foot

Approx. 10mins.
Kyoto St.
Osaka
International Airport (ITM)

Approx. 10mins.
PIAZZA OMI

Zeze St.

Limousine Bus (*2)
Approx. 1h

Notes:*1*2

https://www.kansai-airport.or.jp/en/access/train
https://www.okkbus.co.jp/en/route/itm/

・Your hotel may be in walking distance of the station, but if not (e.g., Prince Hotel, Biwako Hotel),the easiest will be to take a cab.
-MK taxi-Yasaka taxi

+81(0)77-526-4141+81(0)77-522-6767

・If you are going to the Lake Biwa Otsu Prince Hotel, you may be in time for the free shuttle from
Otsu Station (JR Biwako line).

https://www.princehotels.com/otsu/wp-content/uploads/sites/21/2022/11/EN_shuttle-bus_20221121_english-4.pdf

Floor Map

【3F】

1
5

3

ROOM
304

ROOM
305

PIAZZA
HALL

6

2CONFERENCEROOMLOUNGE

4FOYER

LAKE BIWA SIDE
1PIAZZA HALL

4

Opening (17th)
Plenary Sessions (17th, 18th, 19th)
Closing (19th)2

Corporate Exhibition (17th, 18th, 19th)

CONFERENCE ROOM

5

Banquet (17th)
Poster Presentation (18th, 19th)
3

FOYER

ROOM 304
Cloak (17th, 18th, 19th)

ROOM 305

6

RECEPTION
Regstration (17th, 18th, 19th)

Oral Presentation (18th, 19th)
Corporate Seminar (18th, 19th)3

NotesPrecautions● Smoking is only permitted in the designated smoking area.
● Permission from the presenter is required for photography, recording, audio recording, and taking pictures ofboth oral presentations and posters during the presentation.
● Since the contents of the presentations (oral, posters) contain much unpublished data, please refrain frompublishing them outside of this conference without the presenter's consent.

Reception● Registration for the meeting will commence at 12:30 PM on May 17th in front of Piazza Hall on the third floor.
● Please pick up your name tag and give it to the receptionist so they can scan the QR code.

Cloakroom (Luggage storage）● Room 304 is to be utilized as the cloakroom. For further information, please inquire with the cloakroom staff
in Room 304.

Internet connection (Wi-Fi)● We will provide free Wi-Fi access at the conference venue. However, we cannot guarantee a stable andconstant connection for all participants. If you require a stable and high-speed connection, we recommend thatyou bring your own mobile Wi-Fi device, to ensure uninterrupted and reliable internet access.

Accommodation● Please make your own arrangements for accommodation during the meeting. Due to the shortage of hotels
caused by the increase in the number of tourists, it may be difficult to make reservations, so we recommendthat you make reservations for accommodation, including in Kyoto, as soon as possible.

Traffic Information● Venue: Piazza Omi 1-1-20, Nionohama, Otsu, Shiga 520-0801● There are a few parking lots nearby, but we recommend using public transportation as they may be full andparking may not be available. Please refer to the map and direction.

Lunch● There are some restaurants near the venue. Please refer to the following URL and map for more information.
https://www.google.com/maps/d/edit?mid=16jy7ugaAH2fPENyJR4p08eG-OI2niiw&usp=sharing❶ Meeting Venue (Piazza Omi)
❷ Anchov (Pasta)❸ Hashing Diner (Cafe)❹ Chanpon (Ramen)❺ Katsumugi (Port Cutlet）❻ WEST-EAST(Ethnic Cafe)❼ Jukusei-yakiniku Ichiban❽ Moriosumika(Japanese）❾ Chocola (Cafe)❿ Nagisa Warms (Vegan food)⓫ Holly’s Café(Cafe)⓬ Friend Mart (Supermarket)⓭ Yoshinoya Otsu Zeze

⓮ The Meshiya (Japanese dish）⓯ Bikkuri Donkey⓰ Chojiro (Sushi)(Hamburg)⓱ Ion Town (Supermarket)⓲ Kura Sushi（Sushi）⓳ Convenience Store

4

Opening Ceremony
May 17th (Wed) 14:00 - 15:00 (Venue: Piazza Hall)ANEMONE: Japan’s eDNA-based biodiversity monitoring network
Michio Kondoh, Ph.D., Prof. (Tohoku University, Japan)

ANEMONE (All Nippon eDNA Monitoring Network) is a nationwide biodiversity monitoring network in Japanthat uses eDNA metabarcoding. The first eDNA monitoring was initiated by academic scientists in 2015 at
Maizuru Bay, Kyoto, and since then, >5,000 surveys have been conducted at >1,000 sites, identifying a total of
1,000 fish taxonomical groups. The current objective of the network is to monitor fish biodiversity in aquatic
ecosystems, including coastal and pelagic oceans, rivers, and lakes. There are currently 77 fixed monitoring
stations supported by research sites of universities, national research institutes, and local governments, and theirsurvey frequencies vary from weekly to seasonal. Since 2020, hundreds of local citizens have also started
contributing to eDNA-based surveys, and the number of citizen partners is increasing year by year. The
procedure for eDNA metabarcoding, including water collection, filtration, DNA extraction, and molecularanalysis and sequencing, is conducted under a common methodology that follows a standardized protocol
provided by The eDNA Society (https://ednasociety.org/en/manuals/). Claident, a high-throughput analysis
pipeline for metabarcoding data, translates sequence data into taxonomic occurrence, and the data are madeavailable to the public in a dedicated database, ANEMONE DB (https://db.anemone.bio), maintained at Tohoku
University. In June 2022, a consortium of academia, government, and private sectors was established to study
how eDNA monitoring data can provide solutions to a wide range of nature-related social issues.

5Plenary Session 1
May 17th (Wed) 15:30 - 17:30 (Venue: Piazza Hall)
From innovation to practice: Using eDNA to support evidence-based management
Hiroki Yamanaka, Ph.D., Associate Prof. (Ryukoku University, Japan)
The data obtained through environmental DNA (eDNA) analysis is increasing globally year by year. However,to actually utilize the data for biodiversity conservation and fisheries resource management, it is essential tobuild robust and reliable observation systems, including the establishment and maintenance of long-termmonitoring networks and robust programs. In addition, legal and policy frameworks must be updated to allow
the inclusion of eDNA-based data to support management of natural systems. At this symposium, researchers
who have been involved in this series of steps will give an overview of past and current practices, with examples
of current challenges, possible solutions, and a focus on the incorporation of eDNA into long term management.
We will review and invite discussion on all stages of the eDNA workflow from sampling through policy andmanagement.

Hiroki Yamanaka

Elizabeth Andruszkiewicz Allan

Adam Sepulveda

Alison Watts

Ryan Kelly

15:30 - 15:35

Introduction
Hiroki Yamanaka, Ph.D., Associate Prof. (Ryukoku University, Japan)

15:35 - 15:58

The Evolution of eDNA Sampling: From Bottles to Robots
Elizabeth Andruszkiewicz Allan, Ph.D., Chief Scientist (The eDNA Collaborative, USA)

15:58 - 16:21

Transitioning eDNA biomonitoring from research to operational use for invasive species
early detection
Adam Sepulveda, Ph.D., Research Zoologist (United States Geological Survey, USA)

16:21 - 16:44

Establishing Long Term Monitoring Programs to Support Actionable Science andConservation
Alison Watts, Ph.D., Research Faculty (University of New Hampshire, USA)

16:44 - 17:07

Moving eDNA Methods from Knowledge into Action
Ryan Kelly, Ph.D., Associate Prof. (University of Washington, USA)

17:07 - 17:30

Discussion

6Plenary Session 2
May 18th (Thu) 09:00 - 11:00 (Venue: Piazza Hall)
Understanding biodiversity hotspots using eDNA
Hitoshi Araki, Ph.D., Prof. (Hokkaido University, Japan),
Tadashi Kajita, Ph.D., Prof. (University of Ryukyus, Japan)
Biodiversity hotspots are important platforms for a wide variety of ecosystem services. However, our knowledgeof these hotspots and their maintenance is limited due to shortages in budget, workforce, and other factors. In
this symposium, we discuss how to evaluate biodiversity hotspots using eDNA. The topics include organisms inmangrove forests, coral reefs, and open oceans. They illustrate the power of eDNA for biodiversity monitoringand its potential for obtaining a more profound understanding of ecosystems.

Hitoshi Araki

Tadashi Kajita

Christoph Deeg

James Davis Reimer

Sophie von der Heyden

09:00 - 09:15

Monitoring aquatic ecosystems in Hokkaido as a salmonid diversity hotspot in Japan
Hitoshi Araki, Ph.D., Prof. (Hokkaido University, Japan)

09:15 - 09:45

Environmental DNA surveys of Pacific salmon ecosystems from natal rivers to theopen ocean
Christoph Deeg, Ph.D. (University of British Columbia, Canada)

09:45 - 10:15

Using eDNA to understand the diversity of and anthropogenic impacts on coral reefs insouthern Japan
James Davis Reimer, Ph.D., Prof. (University of Ryukyus, Japan)

10:15 - 10:45

Applications of eDNA metabarcoding for baseline biodiversity surveys in southern
African marine and freshwater ecosystems
Sophie von der Heyden, Ph.D., Prof. (Stellenbosch University, South Africa)

10:45 - 11:00

Understanding global mangrove biodiversity by eDNA metabarcoding
Tadashi Kajita, Ph.D., Prof. (University of Ryukyus, Japan; Universiti Malaysia Sabah, Malaysia)

7Plenary Session 3
May 19th (Fri) 09:00 - 11:00 (Venue: Piazza Hall)
Environmental DNA moving from knowledge into practice
Hideyuki Doi, Ph.D., Prof. (Kyoto University, Japan)
Environmental DNA (eDNA) methods for biological monitoring have become increasingly popular over the past
decade. Currently, there is an urgent need to develop survey methods and indicators to assist in understanding
the status of ecosystems. eDNA survey methods, which enable broad-area and high-frequency surveys, cansignificantly contribute to developing these indicators. In this session, we will introduce the latest eDNA surveys
and examples of their social implementation and discuss the potential limitations of such implementation andmeasures to resolve them.

Hideyuki Doi

Florian Leese

Kristy Deiner

Keigo Nakamura

09:00 - 09:10

Environmental DNA moving from knowledge into practice: Introduction
Hideyuki Doi, Ph.D., Prof. (Kyoto University, Japan)

09:10 - 09:50

Advancing biodiversity monitoring with environmental DNA: From method innovation tonationwide implementation across terrestrial and aquatic ecosystems
Florian Leese, Ph.D., Prof. (Duisburg-Essen University, Germany)

09:50 - 10:30

Coordinating a global biodiversity monitoring system with eDNA and the blockchain
Kristy Deiner, Ph.D., Assistant Prof. (ETH Zurich, Switzerland)

10:30 - 11:00

Social implementation of environmental DNA and its barriers in Japan
Keigo Nakamura, Ph.D., Chief Researcher (Riverfront Research Center, Japan)

8Oral Presentation
May 18th (Thu)
Session A (Venue: Piazza Hall)
OA-1
13:00 - 13:15"Making eDNA biodiversity records FAIR (Findable, Accessible, Interoperable andReusable)"
Oliver Berry (CSIRO Environomics Future Science Platform), Simon Jarman (The University ofWestern Australia), Andrew Bissett (CSIRO, Environment), Michael Hope (The Atlas of Living
Australia), Corinna Paeper (The Atlas of Living Australia), Cindy Bessey (CSIRO,
Environment), Michael Schwartz (US National Genomics Center for Wildlife and Fish
Conservation), Josh Hale (Jacobs, Australia), Michael Bunce (Department of Conservation, Te
Papa Atawhai)

OA-2
13:15 - 13:30"Comparison of NCBI gene registration rates for South Korea aquatic organisms for
eDNA research"
Ji-Hoon Kim, Won-Seok Kim, Chang Woo Ji (Chonnam National University), Dong Soo Kong
(Kyonggi University), Ihn-Sil Kwak (Chonnam National University)

OA-3
13:30 - 13:45"An authoritative DNA reference library for Australia’s species"
Jenny Giles (CSIRO Environomics Future Science Platform), Andreas Zwick, Stephen Bent
(CSIRO National Collections and Marine Infrastructure), Troy Denyer (CSIRO Information
Management and Technology), Anna Kearns, James Nicholls, Jesse Wallace (CSIRO National
Collections and Marine Infrastructure), Mark Wallace (CSIRO Environomics Future Science
Platform/CSIRO National Collections and Marine Infrastructure), Claire Yang (CSIRO National
Collections and Marine Infrastructure), Olly Berry (CSIRO Environomics Future Science
Platform)

OA-4
13:45 - 14:00"MitoFish and MiFish Pipeline: recent updates for facilitating environmental DNA
research"
Tao Zhu (The University of Tokyo), Yukuto Sato (The University of the Ryukyus), Tetsuya
Sado, Masaki Miya (Natural History Museum and Institute,Chiba), Wataru Iwasaki (The
University of Tokyo)

OA-5
14:00 - 14:15"MitoSearch: Construction of fish species composition database with environmental DNA
using public data"
Kazutoshi Yoshitake, Takumi Ito, Guanting Liu, Hideaki Mizobata, Shigeharu Kinoshita,
Shuichi Asakawa (The University of Tokyo)

Session B (Venue: Room 305)
OB-1
13:00 - 13:15"Revealing the potential for environmental DNA (eDNA) to detect species interactions in
two economically important plants: Carica papaya and Ananas comosus"
Pritam Banerjee (National Chung Cheng University), Kathryn A. Stewart (Leiden University),
Gobinda Dey, Raju Kumar Sharma (National Chung Cheng University), Jyoti Prakash Maity(KIIT Deemed to be University), Michael W.Y. Chan, ChienYen Chen (National Chung Cheng
University)OB-2
13:15 - 13:30"Using airborne eDNA techniques to track invasive species in high-quality hemlock
forests"
Charlyn Partridge, Megan Sanders, Katherine Geller (Annis Water Resources Institute, Grand
Valley State University), Alexandra Locher (Grand Valley State University), Keely Dunham,
Renee Tardani (Annis Water Resources Institute, Grand Valley State University)9Oral Presentation May 18th (Thu)OB-3
13:30 - 13:45"Comparison of fish fauna evaluated using aqueous eDNA, sedimentary eDNA, and catchsurveys in Tokyo Bay, central Japan"
Mengyao Zhu (Ocean Policy Research Institute of the Sasakawa Peace Foundation), Mari
Kuroki, Tatsushi Kobayashi, Takashi Yamakawa (The University of Tokyo), Tetsuya Sado
(Natural History Museum and Institute, Chiba), Keita Kodama, Toshihiro Horiguchi (National
Institute for Environmental Studies), Masaki Miya (Natural History Museum and Institute, Chiba)

OB-4
13:45 - 14:00"Metabarcoding of modern aquatic and terrestrial vegetation composition from lacustrine
sediment in Yakutia, Eastern Siberia"
Izabella Baisheva (Alfred Wegener Institute Helmholtz Centre for Polar and Marine
Research/University of Potsdam/North-Eastern Federal University/Hokkaido University),
Kathleen R. Stoof-Leichsenring (Alfred Wegener Institute Helmholtz Centre for Polar andMarine Research), Ramesh Glückler (Alfred Wegener Institute Helmholtz Centre for Polar andMarine Research/University of Potsdam/Hokkaido University), Amelie Stieg, Iris Eder (Alfred
Wegener Institute Helmholtz Centre for Polar and Marine Research), Aital Egorov(North-Eastern Federal University), Evgenii S. Zakharov (NorthEastern Federal
University/Siberian Branch of Russian Academy of Sciences), Lena A. Ushnitskaya, Paraskovya
V. Davydova (NorthEastern Federal University), Boris K. Biskaborn (Alfred Wegener InstituteHelmholtz Centre for Polar and Marine Research), Luidmila A. Pestryakova, Sardana N. Levina,
Ruslan M. Gorodnichev (NorthEastern Federal University), Birgit Heim (Alfred Wegener
Institute Helmholtz Centre for Polar and Marine Research), Jorge García Molinos (Arctic
Research Center, Hokkaido University), Ulrike Herzschuh (Alfred Wegener Institute Helmholtz
Centre for Polar and Marine Research/University of Potsdam)

OB-5
14:00 - 14:15"Estimation of paleoenvironment in Lake Shinji through aquatic plant eDNA and CNS
analyses using brackish lake sediment cores"
Satoshi Yamagishi (Shimane University), Koji Seto, Yasuhide Nakamura, Teruhiko Takahara(Estuary Research Center, Shimane University)

Session C (Venue: Piazza Hall)
OC-1
17:15 - 17:30"Hypoxia-associated seasonal variations of zooplankton community in Jinhae Bay, South
Korea: A case study through environmental DNA metabarcoding"
Chang-Bum Jeong, Heesang Shin, In-Cheol Yeo, Kyu-Young Shim, Kyu-Hyung Kim (Incheon
National University)

OC-2
17:30 - 17:45"Oyashio-specific zooplankton survey by eDNA in coastal Sanriku of the Northeast Japan"
Marty Kwok Shing Wong (Atmosphere and Ocean Research Institute, the University of Tokyo),
Yuki Minegishi, Mako Nakao (The University of Tokyo), Yuichiro Nishibe (Atmosphere andOcean Research Institute, The University of Tokyo), Aiko Tachibana (Tokyo University ofMarine Science and Technology), Yoo-Jun Kim, Susumu Hyodo (Atmosphere and Ocean
Research Institute, The University of Tokyo)

OC-3
17:45 - 18:00"Metagenomics and metabarcoding experimental choices and their impact on microbialcommunity characterization in freshwater recirculating aquaculture systems"
Jessica Rieder (University of Bern), Adamandia Kapopoulou, Claudia Bank (University ofBern/Swiss Institute of Bioinformatics), Irene Adrian-Kalchhauser (University of Bern)

OC-4
18:00 - 18:15"Assessment of benthic invertebrate diversity in streams of the Han River using eDNA
metabarcoding"Yong Jun Kim, Chang Woo Ji (Chonnam National University), Dong Soo Kong (Kyonggi
University), Ihn-Sil Kwak (Chonnam National University)OC-5
18:15 - 18:30"What is happening during rainfall? Transition in aquatic/terrestrial invertebrate ratio
detected by eDNA"
Noriko Uchida (Tohoku University)

10Oral Presentation May 18th (Thu)

Session D (Venue: Room 305)
OD-1
17:15 - 17:30"Environmental DNA and RNA detection using Cas13"
Jiwei Yang, Shoma Matsushita, Fei Xia, Susumu Yoshizawa, Wataru Iwasaki (The University ofTokyo)

OD-2
17:30 - 17:45"Application of DNA methylation analysis to eDNA studies"
Itsuki Hirayama, Luhan Wu, Toshifumi Minamoto (Kobe University)

OD-3
17:45 - 18:00"Study on the ayu spawning sites using environmental DNA analysis in the Naka River"
Kensuke Sugimoto, Yoichi Kawaguchi, Takahiro Sato (Tokushima University), Kyosuke
Minami, Shingo Aoki, Koichiro Mizushima, Munehiro Oota (Civil Engineering andEcotechnology Consultants company limited)

OD-4
18:00 - 18:15"The distribution and dispersal of invasive black basses in Japan revealed by eDNA
haplotyping"
Kei Wakimura (Osaka Ohtani University), Shin-ichiro Matsuzaki (National Institute forEnvironmental Studies), Hitoshi Araki (Hokkaido University), Satoshi Kitano (Nagano
Environmental Conservation Research Institute), Yoshiaki Tsuda (University of Tsukuba),
Jun-ichi Tsuboi (Japan Fisheries Research and Education Agency), Tetsu Yatsuyanagi (Hokkaido
University), Tomohiro Kando (University of Tsukuba), Kimiko Uchii (Osaka Ohtani University)

OD-5
18:15 - 18:30"Preserving an Endangered Freshwater Mussel Species through Species-Specific
Environmental DNA Markers"
Hiroki Hata (Ehime University)11Oral Presentation May 19th (Fri)May 19th (Fri)
Session E (Venue: Piazza Hall)
OE-1
13:00 - 13:15"Fish biodiversity across environmental gradient: a comparative approach of eDNA andunderwater visual census (UVC)"
Arthur Chung (The University of Hong Kong), Stan Shea (The ADM Capital Foundation
Ltd./BLOOM Association, Hong Kong), Celia Schunter (The University of Hong Kong)

OE-2
13:15-13:30"Investigation of the effect of Artificial Light at Night (ALAN) on wild fish community~manipulative field experiment and species composition analysis using eDNA methods~"
Aisha Oyabu, Luhan Wu, Takehiro Matsumoto, Natsumi Kihara (Kobe University), Hiroki
Yamanaka (Ryukoku University), Toshifumi Minamoto (Kobe University)

OE-3
13:30 - 13:45"Morphological, DNA barcode and eDNA metabarcoding monitoring of fish in east coastalof North Sumatera and Aceh, Indonesia"
Mohammad Basyuni, Arida Susilowati, Ahmad Baiquni Rangkuti, Desrita Desrita, Ipanna
Enggar Susetya, Siti Halimah Larekeng, Hidayah Abidiah (Center of Excellence for Mangrove,
Universitas Sumatera Utara), Koko Tampubolon (Universitas Sumatera Utara), Yukinobu Isowa,
Tadashi Kajita (Iriomote Station, Tropical Biosphere Research Center, University of the
Ryukyus)OE-4
13:45 - 14:00"Using eDNA metabarcoding to establish targets for freshwater fish composition following
river restoration"
Gen Ito (Ryukoku University), Hiroshi Yamauchi, Miwa Shigeyoshi, Kousuke Ashino, Chie
Yonashiro (Chuo Fukken Consultants), Maki Asami, Yuko Goto (Center for Biodiversity
Science, Ryukoku University), Jeffrey J. Duda (U.S. Geological Survey), Hiroki Yamanaka(Ryukoku University/Center for Biodiversity Science, Ryukoku University)

OE-5
14:00 - 14:15"Evaluation of fish taxonomic and functional diversity in riverine systems using
quantitative environmental DNA metabarcoding"Seiji Miyazono, Michihito Takiyama, Hideaki Miyahira, Ryohei Nakao, Yoshihisa Akamatsu(Yamaguchi University)

OE-6
14:30 - 14:45"Environmental DNA Monitoring for Marine Protected Areas Designation in TsushimaIsland, JAPAN"
Satoquo Seino, Yukihiro Kuratani, Yoko Unoki, Mitsuhiro Aizu-Hirano, Hidefumi Hatashima(Kyushu University)

OE-7
14:45 - 15:00"Fish eDNA survey on Taiwanese waters and marine protected areas"
Wei-Jen Chen (National Taiwan University)

OE-8
15:00 - 15:15"Large scale eDNA database revealing latitudinal diversity gradients with seasonalvariations"
Minoru Kasada, Naoto Shinohara, Riku Fukasawa, Akifumi Tanabe, Michio Kondoh (Tohoku
University)

OE-9
15:15 - 15:30"Spatiotemporal dynamics of eDNA monitoring in Hong Kong marine environments"
Mathew Seymour (The University of Hong Kong)

OE-10
15:30 - 15:45"Spatio-temporal dynamics of fish communities along the coastal region of Boso Peninsula,central Japan: preliminary results from eDNA metabarcoding based on biweekly sampling
for two years"
Ryo Gotoh, Tetsuya Sado, Takehiko Fukuchi, Masaki Miya (Natural History Museum andInstitute, Chiba)

12Oral Presentation May 19th (Fri)

Session F (Venue: Room 305)
OF-1
13:00 - 13:15"Strengthening large-scale biodiversity observation with advances in environmental
genomics"
Nicole Fahner, Avery McCarthy, Beverly McClenaghan, Greg Singer, Mehrdad Hajibabaei(Centre for Environmental Genomics Applications, eDNAtec)

OF-2
13:15 - 13:30"Multiplex approach to ameliorate primer bias in MiFish eDNA metabarcoding: a casestudy of Ayu, Plecoglossus altivelis"
Daisuke Isobe (Ryukoku University), Yusuke Koseki (Otsuma Women’ s University), Hirohiko
Takeshima (Fukui Prefectural University), Hiroki Yamanaka (Ryukoku University)

OF-3
13:30 - 13:45"Primed and ready: Nanopore metabarcoding can now recover highly accurate consensus
barcodes that are almost always indel free"
Jia Jin Marc Chang (National University of Singapore), Yin Cheong Aden Ip (National
University of Singapore/Animal & Veterinary Service, National Parks Board), Wan Lin Neo,Maxine Mowe, Zeehan Jaafar (National University of Singapore), Danwei Huang (National
University of Singapore/Lee Kong Chian Natural History Museum, National University ofSingapore/Centre for Nature-based Climate Solutions, National University of Singapore/
Tropical Marine Science Institute, National University of Singapore)

OF-4
13:45 - 14:00"Environmental DNA study in Insects using the ""MtInsects-16S"", a newly developed
primer for mtDNA 16S region: Achieving highly sensitive detection"
Masaki Takenaka (Shinshu University), Yuta Hasebe (Kanagawa Environmental ResearchCenter), Koki Yano (National Institute for Basic Biology), Koji Tojo (Shinshu University)

OF-5
14:00 - 14:15"Developing lab standard procedures and guidelines to improve uptake of eDNA methods
by resource managers: An overview of two national programs (READI-Net andiTrackDNA)"
Katy Klymus, Margaret Hunter (U.S. Geological Survey, Wetland and Aquatic ResearchCenter), Caren Helbing (University of Victoria), Valerie Langlois (Institut National de laRecherche Scientifique), Jacob Imbrey (University of Victoria), Julie Couillard (Institut
National de la Recherche Scientifique), Adam Sepulveda (U.S. Geological Survey, Northern
Rocky Mountain Science Center)

OF-6
14:30 - 14:45"Putting eDNA science into practice: lessons learned from implementation in citizen
science and business"
Michael Traugott (University of Innsbruck), Daniela Sint (Applied Animal Ecology Research
Unit, Department of Zoology, University of Innsbruck, Austria/Sinsoma GmbH, Austria),
Corinna Wallinger (University of Innsbruck)

OF-7
14:45 - 15:00"Targeting states of eDNA impacts the biodiversity captured and can improve
interpretations of eDNA data"
Anish Ajay Kirtane, Kristy Deiner (ETH Zurich)OF-8
15:00 - 15:15"Challenges of monitoring poorly characterizedmarine and freshwater habitats in
Sub-SaharanAfrica: an eDNA prospect"
Quentin Mauvisseau (Natural History Museum, University of Oslo)

OF-9
15:15 - 15:30"Detection of herbivore fish using environmental DNA: implication for controllingherbivore fish populations toward macroalgal bed restoration"
Masaaki Sato, Tatsuru Kadota, Tsutomu Noda (Japan Fisheries Research and Education
Agency), Masami Hamaguchi (Fukui Prefectural University), Kengo Suzuki (Japan Fisheries
Research and Education Agency)

13

Poster Presentation
May 18th (Thu)

14:30 - 17:00 (14:30 - 16:30 Core time)(Venue: Conference Room)Community structure/Population dynamics
P-01"Temperature sensitivity of the interspecific interaction strength of coastal marine fish
communities"
Masayuki Ushio (Department of Ocean Science, The Hong Kong University of Science andTechnology), Tetsuya Sado, Takehiko Fukuchi (Natural History Museum and Institute, Chiba), Sachia
Sasano (Fisheries Technology Institute, Japan Fisheries Research and Education Agency/Maizuru
Fisheries Research Station, Kyoto University), Reiji Masuda (Maizuru Fisheries Research Station,
Kyoto University), Yutaka Osada (Graduate School of Life Sciences, Tohoku University), Masaki
Miya (Natural History Museum and Institute, Chiba)

P-02"Regional-scale effects of deer-induced forest degradation on river ecosystem dynamics"
Hikaru Nakagawa (Aqua Restoration Research Center, Public Works Research Institute), Daisuke
Fujiki (Institute of Natural and Environment Science, University of Hyogo/Wildlife Management
Research Center, Hyogo), Hiroo Numata (Wildlife Management Research Center, Hyogo), Luhan Wu(Graduate School of Human Development and Environment, Kobe University), Terutaka Mori (Aqua
Restoration Research Center, Public Works Research Institute), Toshifumi Minamoto (Graduate Schoolof Human Development and Environment, Kobe University)

P-03"Integrating bacterial DNA metabarcoding, EcoPlate metabolic profiling, and sparse regression
modeling to predict ecosystem function sensitivity to biodiversity loss"
Wan-Hsuan Cheng (Taiwan International Graduate Program -Earth System Science Program,
Academia Sinica/Taiwan International Graduate Program -Earth System Science Program, National
Central University/Center for Biodiversity Science, Ryukoku University), Takeshi Miki (Center for
Biodiversity Science, Ryukoku University), Chih-hao Hsieh (Institute of Oceanography, National
Taiwan University/Research Center for Environmental Changes, Academia Sinica/National Center for
Theoretical Sciences, Mathematics Division/Institute of Ecology and Evolutionary Biology,
Department of Life Science, National Taiwan University), Chun-Wei Chang (Institute of Fisheries
Science, National Taiwan University/National Center for Theoretical Sciences, Mathematics Division),
Fuh-Kwo Shiah (Research Center for Environmental Changes, Academia Sinica)

P-04"Characteristics of assemblages and species richness of Sebastes rockfishes along the coasts ofnorthern Japan"
Tetsu Yatsuyanagi, Takashi Kanbe (Hokkaido University), Hiroki Mizumoto (Hokkaido
University/Japan Fisheries Research and Education Agency), Shouko Inoue, Hitoshi Araki (Hokkaido
University)

P-05"The effect of climate fluctuation on microbial biodiversity in soil and rice paddy fieldecosystem"
Takuya Kageyama (Center for Ecological Research, Kyoto University), Kenta Suzuki (RIKEN
BioResource Research Center), Hirokazu Toju (Center for Ecological Research, Kyoto University)

P-06"Elucidating the Relationship Between Environmental Conditions and Alternative Stable Statesin a Actual Microbial Community Using Quantitative DNA Metabarcoding"
Genta Shima, Hiroaki Fujita, Hrikazu Toju (Center for Ecological Research, Kyoto University)

P-07"Factors Affecting Variation in Seed Bacterial Communities of Wild Tree, Mallotus japonicus"
Marin Komojiri (Center for Ecological Research, Kyoto University), Masayuki Ushio (The Hong
Kong University of Science and Technology), Kazuya Takeda (The University of Tokyo), Shoko Sakai(Center for Ecological Research, Kyoto University)

14

Poster Presentation May 18th (Thu)
P-08"Integrated community analysis using mass DNA metabarcoding revealed close relationships
among plant root-associated fungi"
Mikihito Noguchi, Hirokazu Toju (Center for Ecological Research, Kyoto University)

LBP-09 "Characterization of floral bacteria by their context-dependency and host specificity"
Kazuya Takeda (The Botanical Garden, Graduate School of Science, The University of Tokyo/Center
for Ecological Research, Kyoto University), Shoko Sakai (Center for Ecological Research, Kyoto
University)

Methodology
P-10"Water drunk by raccoons: how long can their DNA be detected?"
Chinatsu Kozakai (National Agriculture and Food Research Organization), Hiroyoshi Koyama(Saitama Agricultural Technology Research Center), Hirotaka Matsumura (Chiba Prefecture), Ryusuke
Fujimoto, Hiroyuki Shibaike (National Agriculture and Food Research Organization)

P-11"The environmental RNA of fishes, algae, and arthropods is an effective indicator of ecologicalsurvey and water quality assessment in rivers"
Kaede Miyata, Yasuaki Inoue, Yuto Amano, Tohru Nishioka (R&D Safety Science Research, Kao
Corporation), Tomohisa Nagaike, Takamitsu Kawaguchi (Bioindicator Co., Ltd.), Osamu Morita,
Hiroshi Honda, Masayuki Yamane (R&D Safety Science Research, Kao Corporation)

P-12"Assessing environmental nucleic acid pollution in freshwater bodies by domestic wastewater
toward simulation of the distribution of nucleic acid causing false positives"
Yasuaki Inoue, Kaede Miyata, Masayuki Yamane, Hiroshi Honda (R&D Safety Science Research, Kao
Corporation)

P-13"Development and Evaluation of microfluidic filtration extraction method (Biryu-Chip
method)"Takashi Fukuzawa (GoFoton/Biryu Planning), Naofumi Nishizawa, Hisao Nagata, Hiromi Shirakura(GoFoton), Hideyuki Doi (Kyoto University)

P-14"Environmental DNA metabarcoding of benthic invertebrates in riverine environments: amodel-based assessment of species detectability for optimized study design"
Keiichi Fukaya (National Institute for Environmental Studies), Yuta Hasebe (Kanagawa Environmental
Research Center)

P-15"Detection of Southeast Asian liver fluke by multi-region PCR assay"
Riko Matsuo (Graduate School of Human Development and Environment, Kobe University), Megumi
Sato (Graduate School of Health Sciences, Niigata University), Mercello Sato (Niigata University of
Pharmacy and Mediccal and Life Siecnces), Poom Adisakwattana, Orawan Phuphisut, Tippayarat
Yoonuan (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University), Yanin
Limpanont, Yupa Chusongsang, Paporn Poodeepiyasawat (Department of Social and Environmental
Medicine, Faculty of Tropical Medicine, Mahidol University), Toshifumi Minamoto (Graduate Schoolof Human Development and Environment, Kobe University)

P-16"Zooplankton sedimentary DNA as an effective tool for tracking past population dynamics"
Narumi Tsugeki (Faculty of Law, Matsuyama University), Kai Nakane (Center for Marine
Environmental Studies, Ehime University), Hideyuki Doi (Graduate School of Informatics, Kyoto
University), Kazuaki Tadokoro (Shiogama Field Station, Fisheries Resource Institute), Michinobu
Kuwae (Center for Marine Environmental Studies, Ehime University)

P-17"Environmental mRNA-seq of Xenopus laevis and Gasterosteus aculeatus"
Fei Xia, Jiwei Yang, Yuanyu Wang (Graduate School of Frontier Sciences, The University of Tokyo),
Yihan Ma (Faculty of Environment and Information Studies, Keio University), Susumu Yoshizawa(Graduate School of Frontier Sciences, The University of Tokyo), Hiroki Kuroda (Graduate School ofMedia and Governance, Keio University), Asano Ishikawa, Wataru Iwasaki (Graduate School of
Frontier Sciences, The University of Tokyo)

15

Poster Presentation May 18th (Thu)
P-18"Differences in concordance ratios between environmental DNA metabarcoding and capture
surveys among fish species"
Kazuki Kanno, Ryusuke Shinohara, Souta Nakajima, Keiko Muraoka (Watershed Restoration Team,
Water Environment Research Group, Public Works Research Institute), Masashi Kanaya (River
Environment Division, Water and Disaster Management Bureau, Ministry of Land, Infrastructure,
Transport and Tourism), Kazutaka Sakiya (Watershed Restoration Team, Water Environment ResearchGroup, Public Works Research Institute)

P-19"Evaluation of the efficacy of sponges as a passive environmental DNA sampler"
Ryohei Nakao, Manami Inaba, Seiji Miyazono (Graduate School of Science and Technology for
Innovation, Yamaguchi University), Minoru Saito (Japan International Research Center for
Agricultural Sciences/Graduate School of Science and Technology for Innovation, Yamaguchi
University), Keita Maruyama, Yoshihisa Akamatsu (Graduate School of Science and Technology for
Innovation, Yamaguchi University)

P-20"Use of environmental DNA for seasonal monitoring of invasive aquatic plant Egeria densa in ariverine system"
Hideaki Miyahira, Seiji Miyazono, Yoshihisa Akamatsu (Yamaguchi University)

P-21"The eDNA analysis using rainwater to understand forest canopy biota"
Takumaru Miwa (University of Hyogo), Hideyuki Doi (Kyoto University), Naoya Miyashita (Himeji
City Science Museum)

P-22"Development of species-specific qPCR assays for detecting raccoon (Procyon lotor) eDNA fromfield water"
Hiroyoshi Koyama (Saitama Agricultural Technology Research Center), Chinatsu Kozakai (National
Agriculture and Food Research Organization), Hirotaka Matsumura (Chiba Prefecture), Hiroyuki
Shibaike (National Agriculture and Food Research Organization)

P-23"Environmental DNA Surveillance of Biocontamination in a Drinking Water Treatment Plant"
Kyu-Young Shim, Heesang Shin, In-Cheol Yeo (Department of Marine Science, College of Natural
Sciences, Incheon National University), Kyu Ri Kim (Hoseo Toxicology Research Center, Hoseo
University), In-Sil Kwak (Department of Ocean Integrated Science, Chonnam National University),
Chang-Bum Jeong (Department of Marine Science, College of Natural Sciences, Incheon National
University)

P-24"Validation of metabarcoding using eDNA to detect bivalves of Unionidae"
Natsuko Kondo, Kazuya Nishida, Hiroshi Ito, Makoto Yoshida, Kohji Mabuchi (National Institute forEnvironmental Studies)

P-25"Photocontrolled one-pot RPA-CRISPR-Cas12a assay for Detection of Bahaba taipingensis from
Environmental DNA"
Junjie Wang, Jinxian Wu, Jiabo Chen (School of Life Science, South China Normal University), Xiao
Chen (South China Agriculture University), Jun Zhao, Xiaoming Zhou (School of Life Science, South
China Normal University)

P-26"Detection of eDNA from unionid bivalve Nodularia nipponensis using multi-copy nuclear DNA
marker"Kotaro Sugawara (Lake Biwa Museum), Sota Sugawara, Kunihiro Okano, Miho Watanabe, Naoyuki
Miyata (Akita Prefectural University)

P-27"Design of an Agricultural Environment Genetic Metabarcode Database (DB) Platform"
Keonhee Kim (Konkuk University), Junghwan Park (PJ Factory Ltd.)

P-28"Attached DNA on tree leaves as a source for eDNA metabarcoding analysis on terrestrial
vertebrates"
Soutarou Miyake, Koki Kinoshita, Takashi Fujimori, Hiroki Yamanaka (Ryukoku University)

16

Poster Presentation May 18th (Thu)
P-29"Targeted PCR is more effective to detect Eurasian otters (Lutra lutra) than metabarcoding- A
case study of f Yeoido Ecological Park, Seoul, Korea"
Yujin Kang (Interdisciplinary Program in Landscape Architecture, Seoul National University),
Jeoungeun Jeon, Youngkeun Song (Department of Landscape Architecture, Graduate School ofEnvironmental Studies, Seoul National University)

P-30"Comparison of environmental DNA analysis and traditional method (capture survey): For
efficient fish surveys"
Takaya Hirohara, Masatoshi Hayashi, Tomoki Nakamichi, Takahiko Okamura, Takanori Nishikawa,
Masahiro Ono (KANSO TECHNOS CO., LTD.)

P-31"Application of eDNA in lake ecosystem assessment: use of eDNA metabarcoding on thedevelopment of zooplankton index for health assessment"
Yerim Choi (Department of Environmental Science and Engineering, Kyung Hee University), Chang
Woo Ji (Fisheries Science Institute, Chonnam National University), Hye-Ji Oh, Geun-Hyeok Hong,
Hyunjoon Kim, Jong Min Oh (Department of Environmental Science and Engineering, Kyung Hee
University), Ihn-Sil Kwak (Department of Ocean Integrated Science, Chonnam National
University/Fisheries Science Institute, Chonnam National University), Young-Seuk Park (Departmentof Biology, Kyung Hee University), Kwang-Hyeon Chang (Department of Environmental Science andEngineering, Kyung Hee University)

P-32"Development and availability of a local DNA database for DNA meta-barcoding of aquatic
insects"
Sakiko Yaegashi, Aoi Hamamoto, Yuto Sasaki (University of Yamanashi), Yuta Hasebe (Kanagawa
Environmental Research Center)

P-33"A feasibility study of automated underwater sampling devices to analyze environmental andmicrobial DNA"
Shuji Goto (Atmosphere and Ocean Research Institute, The University of Tokyo), Tatsuhiro Fukuba(Institute for Marine-Earth Exploration and Engineering, Japan Agency for Marine-Earth Science andTechnology/Institute of Industrial Science, The University of Tokyo), Marty Kwok-Shing Wong, Yuki
Minegishi, Susumu Hyodo, Yoko Makabe-Kobayashi, Yota Sugai, Koji Hamasaki (Atmosphere andOcean Research Institute, The University of Tokyo)

P-34"Detection of the Japanese sea nettle Chrysaora pacifica through RPA-CRISPR-Cas12a
environmental DNA assay and its field validation in Jinhae Bay, South Korea"
Kyuhyeong Kim, Usha Jyoti Maji, Kyu-Young Shim, In-Cheol Yeo, Su-Eon Song, Chang-Bum Jeong(Department of Marine Science, College of Nature Science, Incheon National University)

P-35"Investigating a method for detecting environmental DNA from rare wildlife species by a mobile
PCR platform"
Toshiya Suzuki, Kazuki Toida, Naoya Ishiguro (Josai University)

P-36"Improving environmental DNA sampling in rice paddies"
Satoshi Yamamoto, Naoki Katayama, Junsuke Yamasako, Kenji Ito, Hiroaki Ikeda, Yuki Baba
(National Agriculture and Food Research Organization)

P-37"Maximizing the potential of eDNA samples: using whole genome amplification technologies toexpand the possibilities of downstream analyses"
Kimiko Uchii, Kana Taguchi (Faculty of Pharmacy, Osaka Ohtani University), Tomoaki Ichijo (Facultyof Health and Nutrition, Osaka Shoin Women’s University), Masao Nasu (Graduate School of
Pharmaceutical Sciences, Osaka Ohtani University)

P-38"Optimization of sampling methods for detection of fish environmental DNA in the open ocean"
Akihide Kasai, Makoto Ozaki, Tatsuya Kawakami, Hiromichi Ueno (Faculty of fisheries sciences,
Hokkaido University)

17

Poster Presentation May 18th (Thu)
P-39"Development and practice application of Asian Black Bear (Ursus thibetanus japonicus)
monitoring methods using airborne eDNA"
Kazushi Masuda (Graduate School of Innovation and Practice for Smart Society, Hiroshima
University), Mikio Nishihara, Fumika Ito (Graduate School of Integrated Sciences for Life, Hiroshima
University), Jun Hatase, Ayako Noda (Hiroshima City Asa Zoological Park), Hiroshi Yasue (Tsukuba
Gene Technology Laboratories Inc.), Masahide Nishibori (Graduate School of Innovation and Practicefor Smart Society, Hiroshima University/Graduate School of Integrated Sciences for Life, Hiroshima
University)

P-40“Understanding the ecology of eRNA toward the improvement of eRNA analysis”
Toshiaki Jo (Ryukoku University)

LBP-41 "Modeling and simulation of environmental DNA dynamics"
Tatsuya Saito, Hideyuki Doi (Graduate School of Informatics, Kyoto University)
LBP-42 "Development of a highly sensitive eDNA detection method"
Ryo Iwamoto, Tomohiro Kuroita (Shionogi & Co. Ltd./AdvanSentinel Inc.), Yuta Kunimasa, Yuna
Yamamoto, Toshifumi Minamoto (Graduate School of Human Development and Environment, Kobe
University)
LBP-43 "High-Resolution Insights into Microbiomes under Stress: The Promise of eDNA Approaches?"
Jinping Cheng (The Education University of Hong Kong)
LBP-44 “Challenges and progress in the application of eDNA metabarcoding as a biomonitoring tool for
mangrove-associated fauna in the Philippines”
Venus Leopardas, Ruby Gonzales, Nonillon Aspe, Allyn Duvin Hinoguin (Mindanao State University
at Naawan), Joel Hassan Tolentino, Joan Acaso (Philippine Genome Center, University of thePhilippines Mindanao), Roger Dolorosa (College of Fisheries and Aquatic Sciences, Western
Philippines University), Renan Bobiles (Coastal Resource Management Section, Bicol University Tabaco Campus), Thesa Mendoza (Aklan State University - New Washington Campus)
LBP-45 "Applicability of environmental DNA metabarcoding for the hyporheic zone of a stream bed"
Izumi Katano (Faculty, Division of Natural Science, Nara Women’s University), Aki Tanaka(KYOUSEI Science Center, Nara Women’s University), Hideyuki Doi (Graduate School ofInformatics, Kyoto University), Mami Iguchi (Department of Chemistry, Biology, and Environmental
Science, Faculty of Science, Nara Women’s University), Shohei Koike (School of Human Science andEnvironment, University of Hyogo)

Spatiotemporal variation
P-46"Seasonal environmental DNA analyses of rivers in Fukuoka Prefecture"
Shusaku Hirakawa, Jun Nakajima (Fukuoka Institute of Health and Environmental Sciences)

P-47"Fish population dynamics in Lake Akan associated with eutrophication reconstructed by
sedimentary DNA"
Masayuki K. Sakata (Research Faculty of Agriculture, Hokkaido University/Graduate School of
Human Development and Environment, Kobe University), Michinobu Kuwae (Center for Marine
Environmental Studies, Ehime University), Hajime Ohtsuki (Graduate School of Life Sciences,
Tohoku University), Isamu Wakana (Kushiro International Wetland Centre), Toshifumi Minamoto(Graduate School of Human Development and Environment, Kobe University), Jotaro Urabe(Graduate School of Life Sciences, Tohoku University)

P-48"Seasonal movement of Rainbow Trout detected by year-round water sampling"
Akio Imamura (Hokkaido University of Education), Masayuki K Sakata (Hokkaido University),Toshifumi Minamoto (Kobe University)

18

Poster Presentation May 18th (Thu)P-49"Intra-annual fauna variation in urban wetlands detected by eDNA metabarcoding and camera
trapping"
Jiyoon Park (Department of Landscape Architecture, Graduate School of Environmental Studies, Seoul
National University), Yujin Kang, Seungwoo Han (Interdisciplinary Program in LandscapeArchitecture, Seoul National University), Jeongeun Jeon, Youngkeun Song (Department of LandscapeArchitecture, Graduate School of Environmental Studies, Seoul National University)

P-50"Seasonal variation of fish fauna in Otsuchi Bay by environmental DNA metabarcoding
analysis"
Mai Teruya (Iwate University), Yuki Minegishi (The University of Tokyo)

Diet
P-51

"The combinational analyses of environmental and stomach content DNA may reveal piscivoryof the Japanese Spiny Lobster (Panulirus japonicus)"
Shoju Shi, Reiji Masuda (Maizuru Fisheries Research Center, Kyoto University)

P-52"Seasonal- and Size- dependent foraging by the masu salmon (Oncorhynchus masou) revealed by
DNA metabarcoding"
Yumiko Ishii, Jaeick Jo (National Institute for Environmental Studies), Tsutomu Kanasashi, Toshihiro
Wada (Fukushima University), Natsuko Kondo, Nobuyoshi Nakajima (National Institute forEnvironmental Studies), Rie Saito (University of Georgia), Masanori Tamaoki, Seiji Hayashi (National
Institute for Environmental Studies)

P-53"Application of DNA Metabarcoding for identifying the potential food of Corbicula (Asian Clam)in the lower part of the Seomjin and Nakdong River, Korea"
Yu-Ji Heo (Department of Environmental Education, Sunchon National University), Kang-Hui Kim,
Hyunbin Jo (Department of Integrated Biological Science, Pusan National University), Hyun-Woo
Kim (Department of Environmental Education, Sunchon National University)

P-54"Prey Species for Yellowjacket Wasps (Vespula shidai) Raised for Consumption by Ordinary
Citizens in Central Japan and Wild Nests"
Tatsuya Saga (Graduate School of Human Development and Environment, Kobe University)

Distribution
P-55"Development of qPCR based eDNA detection assay to assess the distribution of a fish species inthe Himalayan hill stream"
Rishikesh Krishan Laxmi, Varunendra Singh Rawat, Nitika Sharma, Neeta Sehgal (Department of
Zoology, University of Delhi)

P-56"Differences in vertical distribution of two salmonid species: the impacts of warm watertemperature and hypoxia in a stratified lake"
Kayoko Fukumori, Natsuko I. Kondo, Ayato Kohzu, Kenji Tsuchiya, Hiroshi Ito, Taku Kadoya(National Institute for Environmental Studies)

P-57"Distribution of finless porpoise (Neophocaena asiaeorientalis) in Osaka bay, Japan using eDNA
analysis"
Nagisa Hashimoto (Graduate School of Human Development and Environment, Kobe University),
Takashi Iwata (Graduate School of Maritime Sciences, Kobe University), Toshifumi Minamoto(Graduate School of Human Development and Environment, Kobe University)

P-58"Distribution analysis of the invasive diatom Cymbella janischii using environmental DNA frombenthic diatoms in river systems"
Yoko Kato-Unoki (Kyushu University), Tamie Suzawa (Institute of River Biology Ltd), Tran Thu
Trang (Kyushu University), Yoshitsugu Masuda, Jun-ichi Tsuboi (Japan Fisheries Research and
Education Agency), Satoquo Seino (Kyushu University)

19

Poster Presentation May 18th (Thu)
P-59"Surveying the Habitat of Blotched tabira Bitterling and their Spawning Host Mussels Using
Environmental DNA: A Novel Methodological Approach"Kazuyuki Ooi, Tomoyuki Jouuchi (Kyushu Environmental Evaluation Association)

P-60"Unraveling the effects of cross river structures on the habitat of Japanese eel"
Yuta Kunimasa, Natsumi Kihara, Toshifumi Minamoto (Graduate School of Human Development andEnvironment, Kobe University)

P-61"Investigation of eDNA Quantitative Analysis Method for Understanding Fish Dynamics inMountain Streams"
Shiori Nagahora, Masateru Nishiyama (Department of Food, Life and Environmental Sciences, Facultyof Agriculture, Yamagata University), Takahiro Sato (Fukken Gijyutsu Consultants Co., Ltd), Kazuya
Watanabe (Department of Food, Life and Environmental Sciences, Faculty of Agriculture Yamagata
University)

P-62"Comparative phylogeography by environmental DNA analysis: investigating the relationshipbetween phylogeographic patterns and ecological traits in Rhinogobius species"
Satsuki Tsuji, Shota Kunimatsu, Katsutoshi Watanabe (Kyoto University)

P-63"Characterization of the distribution of the flagship species, Gobiobotia nakdongensis, within thestream basin where the dam was constructed: application of species-specific markers andtargeted qPCR methods"
Hye-Ji Oh (Department of Applied Environmental Science, Kyung Hee University), Keun-Sik Kim(Research Center for Endangered Species, National Institute of Ecology), Keun-Yong Kim(AquaGenTech Co., Ltd), Jeongwoo Yoo (Research Center for Endangered Species, National Instituteof Ecology), Kyu-Jin Kim, Min-Ho Jang (Department of Biology Education, Kongju National
University), Jeong-Hui Kim (EcoResearch incorporated), Doo-Hee Won (Doohee Institute of
Ecological Research, Korea Ecosystem Service Inc.), Ju-Duk Yoon (Research Center for Endangered
Species, National Institute of Ecology), Kwang-Hyeon Chang (Department of Applied Environmental
Science, Kyung Hee University)

P-64"Species distribution of Korean raccoon dog in urban wetland using eDNA and conventional
survey"Seungwoo Han (Interdisciplinary Program in Landscape Architecture, Seoul National University),
Jeongeun Jeon (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul
National University), Yujin Kang (Interdisciplinary Program in Landscape Architecture, Seoul National
University), Youngkeun Song (Dept. of Landscape Architecture, Graduate School of Environmental
Studies, Seoul National University/Transdisciplinary Program in Smart City Global Convergence,
Seoul National University)

P-65"Distribution estimation of Cervus nippon taionaus using environmental DNA (eDNA)"
Suyeon Won, Yujin Kang, Suengwoo Han, Jeongeun Jeon, Jiwoon Park (Seoul National University),
Shinjae Rhim (Chung Ang University), Youngkeun Song (Seoul National University)

P-66"Use of quantitative environmental DNA metabarcoding for grasping the habitat of threatened
fish species in the first class rivers of the Chugoku District"
Michihito Takiyama, Seiji Miyazono, Hideaki Miyahira, Ryohei Nakao, Takumi Hanaoka, Yoshihisa
Akamatsu (Yamaguchi University)

P-67"Efficient assessment of cryptic invasions using environmental DNA: the case of the minnow
Pseudorasbora parva in Japan"
Atsushi Kogayu, Satsuki Tsuji, Katsutoshi Watanabe (Department of Biology, Graduate School of
Science, Kyoto University)

20

Poster Presentation May 18th (Thu)
P-68"Horizontal and vertical distribution of environmental DNA of jack mackerel Trachurusjaponicus and Japanese anchovy Engraulis japonicus in Maizuru Bay, Kyoto Prefecture"
Hiroaki Murakami (Tohoku University), Keiichi Fukaya (National Institute for Environmental
Studies), Satoshi Yamamoto (National Agriculture and Food Research Organization), Toshifumi
Minamoto (Kobe University), Kenji Minami, Kazushi Miyashita (Hokkaido University), Taishin
Kameoka (Japan International Research Center for Agricultural Sciences), Reiji Masuda, YohYamashita (Kyoto University), Michio Kondoh (Tohoku University)

LBP-69 "An environmental DNA survey reveals orthotopic habitat of Anguilla japonica and A.
marmorata in Nagasaki, Japan"
Yurika Ono, Katsuya Hirasaka (Graduate School of Fisheries Science and Environmental Studies,
Nagasaki University), Taijun Myosho (Department of Environmental and Life Sciences, Faculty ofFood and Nutritional Sciences, University of Shizuoka), Shingo Fujimoto (Tropical Biosphere
Research Center, University of the Ryukyus), Mitsuharu Yagi (Graduate School of Fisheries Scienceand Environmental Studies, Nagasaki University)
LBP-70 "Detection of spatio-temporal change in herbivorous fish distribution around the coast of Japan
using ANEMONE eDNA data"
Chris Bayne, Mahiko Abe (Japan Fisheries Research and Education Agency), Toshinori Baba
(Yamaguchi Prefectural Fisheries Research Center), Hideki Hamaoka (Niigata Prefectural Fisheries
and Marine Research Institute), Natsuki Hasegawa (Japan Fisheries Research and Education Agency),
Hiroyo Igari (Kagoshima Prefectural Fisheries Technology and Development Center), Mitsuhiro Ishii(Chiba Prefectural Fisheries Research Center), Atsushi Ito (Japan Fisheries Research and Education
Agency), Yusuke Marume (Miyazaki Prefectural Fisheries Research Institute), Toshihiro Nishioka(Kagawa Prefectural Fisheries Experiment Station), Masaaki Sato (Japan Fisheries Research and
Education Agency), Shiyouichi Takenaka (Fisheries Research Center, Ehime Research Institute ofAgriculture, Forestry and Fisheries), Yoshiyuki Tanaka (Hachinohe Institute of Technology), Hideaki
Yamada (Japan Fisheries Research and Education Agency), Masayuki Yamamoto (Kagawa Prefectural
Fisheries Experiment Station/Fukui Prefectural University), Kosuke Yatsuya (Japan Fisheries Researchand Education Agency), Keiichiro Yoshimi (Fisheries Research Division Tokushima Agriculture,
Forestry, and Fisheries Technology Support Center), Satoshi Nagai (Japan Fisheries Research and
Education Agency), Masami Hamaguchi (Fukui Prefectural University), Masakazu Hori (Japan
Fisheries Research and Education Agency/Tokyo University of Marine Science and Technology)

Diversity
P-71"Comprehension of Species Compositions Using Environmental DNA Metabarcoding in Beppu
Bay Sediment"
Chisato Numa (Graduate School of Information Sciences, University of Hyogo)

P-72"Non-destructive monitoring of arthropod community on woody plant, Quercus serrata, using
environmental DNA metabarcoding"Kinuyo Yoneya (Kindai University/Center for Biodiversity Science, Ryukoku Univ.), Takeshi Miki(Center for Biodiversity Science, Ryukoku University), Akio Kashimura (Kindai University)

P-73"Classification of abandoned rice paddy fields using environmental variables and eDNA
metabarcoding of Amphibia"
Jeongeun Jeon (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul
National University), Yujin Kang, Seungwoo Han (Interdisciplinary Program in LandscapeArchitecture, Seoul National University), Jiyoon Park (Dept. of Landscape Architecture, Graduate
School of Environmental Studies, Seoul National University), Youngkeun Song (Interdisciplinary
Program in Landscape Architecture, Seoul National University/Transdisciplinary Program in Smart
City Global Convergence, Seoul National University)

21

Poster Presentation May 18th (Thu)
P-74"Monitoring fish biodiversity using eDNA metabarcoding in a World Natural Heritage Island,
Iriomote, Okinawa, Japan"
Bernadeth Grace Pananganan, Yukinobu Isowa, Maria Daniela Artigas Ramirez (Iriomote Station,
Tropical Biosphere Research Center, University of the Ryukyus), Takashi Yamamoto (Tama University
Hijirigaoka High & Junior High School), Masaki Miya (Natural History Museum and Institute, Chiba),
Tadashi Kajita (Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah,
Malaysia/Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus/United
Graduate School of Agricultural Sciences, Kagoshima University)

P-75"Environmental DNA metabarcoding for Measuring Fish Species Diversity in Pearl River
Estuary surrounding Chinese Bahaba nature reserve ecosystems during the migration andnon-migration period"
Jinxian Wu，Jiabo Chen，Xiao Chen，Lei Zhou，Xuehui Wang，Jun Zhao，Junjie Wang

P-76

"eDNA metabarcoding of water stream flowing the Japanese garden in Kyoto"
Kazutoshi Tsuda (Kyoto Institute of Technology/Yamaguchi Center for Arts and Media), Fumie
Takahara (Yamaguchi Center for Arts and Media), Tomohiro Inoue (Kyoto Institute of Technology),
Takayuki Ito (Yamaguchi Center for Arts and Media)

P-77"Investigating intraspecific and species-level diversity in marine species using COI DNA
metabarcoding: a case study of Zostera sp. community in the northern Sea of Japan?"
Sergei Turanov (A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch,
Russian Academy of Sciences)P-78"Environmental DNA as an assessment tool for wetland biodiversity in Heuksando, Korea"
Gawoo Kim (Interdisciplinary Program of Landscape Architecture, Seoul National University)

P-79"Investigation of the potential applicability of eDNA as an environmental education tool"
Ryota Kitani, Mieko Kiyono, Masayuki Sato, Atushi Ushimaru, Toshifumi Minamoto (Graduate
School of Human Development and Environment, Kobe University)

LBP-80 "Detection of the Fish Biodiversity in Mangrove Ecosystem in Nakara River at Iriomote Island,
Japan using MiFish eDNA Metabarcoding"Severino G. Salmo III (Institute of Biology, College of Science, University of the Philippines Diliman),
Yukinobu Isowa (Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus),
Takashi Yamamoto (Iriomote Station, Tropical Biosphere Research Center, University of the
Ryukyus/Faculty of Science, University of the Ryukyus /Tama University Hijirigaoka High & JuniorHigh School), Tetsuya Sado (Department of Ecology and Environmental Sciences, Natural History
Museum and Institute Chiba), Katsuya Ito (Faculty of Science, University of the Ryukyus),
Mohammad Basyuni (Center of Excellence for Mangrove, Universitas Sumatera Utara), Shinya Imura,
Keiichi Ishigaki (Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus),
Masaki Miya (Department of Ecology and Environmental Sciences, Natural History Museum andInstitute Chiba), Tadashi Kajita (Iriomote Station, Tropical Biosphere Research Center, University of
the Ryukyus/Graduate School Union of Agriculture, Kagoshima University/Institute for Tropical
Biology and Conservation, Universiti Malaysia Sabah)

22

Corporate Workshop 1
Date: May 18th (Thu), 11:00 - 12:30 (Venue: Room 305)

Explanation: (Illumina K.K.)

15-minute company talk from Illumina is planned at the beginning of the workshop.

OceanOmics: Bolstering marine research and conservation with genomic technologies
Priscila Goncalves
Minderoo Foundation • Flourishing Oceans
The world’s ocean is the largest, least understood and yet one of the most exploited realms on our planet.
Although the ocean covers around 71% of Earth’s surface and supports 90% of its biosphere, more than 80% of
the ocean is unmapped, unexplored and unobserved. The OceanOmics program at the Minderoo Foundation set
out to transform how we observe, measure and understand life in our ocean to ultimately accelerate ocean
conservation and halt biodiversity loss. We believe that eDNA-based marine genomics can form thetechnological basis for innovations that can revolutionise how we collect biodiversity data across space and time.
We are working with globally renowned scientists and industry partners to advance eDNA-based technologies
for biodiversity monitoring in these vast and highly dynamic ocean environments. This involves optimisationand scaling of eDNA collection, processing, and characterisation, including the development of novel
approaches for species detection and quantification. To enable ocean-scale eDNA-based biodiversity surveys,
OceanOmics is committed to generating and openly publishing reference genomic resources for thousands ofspecies of marine vertebrates, as well as developing the most comprehensive analytical tools for marine
sequencing data based on standardised, reproducible processes and artificial intelligence. These evolving
technologies have been deployed at sea from a unique marine genomics platform, which houses onboard
high-throughput sequencing and bioinformatics resources. Over the last two years, this vessel has enabledmultiple research expeditions across Australia, and represents a powerful asset for the establishment of
biodiversity baselines and long-term biomonitoring programs. The development and application of suchcomprehensive genomic tools and resources will facilitate the rapid, accurate and large-scale generation of
biodiversity data to support ocean management and conservation efforts.

Collaborative eDNA initiatives in Australia and New Zealand
Oliver BerryCSIRO Environomics Future Science Platform
Southern eDNA Society
Environmental DNA research and application is rapidly evolving globally, including in Australia and New
Zealand. As this Australasian eDNA community has grown and matured we have begun to address sharedbarriers to technical feasibility and adoption by government and industry. In this presentation I will introduce
four initiatives that illustrate this collective approach and that have international analogues or relevance: 1) theestablishment of the Southern eDNA Society - a society advocating for best practice and adoption of eDNA
science in the region; 2) the first Australian and New Zealand eDNA conference held in February 2023; 3) The
National Biodiversity DNA Library – a library of complete DNA reference sequences for all named Australian
species; and 4) the Making eDNA FAIR project – data standards that enable eDNA data sharing. The objectivesof my presentation are to create awareness of eDNA activities in Australia and New Zealand, seek feedback andcontributions, and to encourage collaborations that will grow the cohesiveness and effectiveness of thisremarkable and much needed technology around the world.

*Lunch box will be provided for those who pre-register.

23

Corporate Workshop 2
Date: May 19th (Fri), 11:00 - 12:30 (Venue: Room 305)

Explanation: Naofumi Nishizawa (President, Go!Foton, Inc.)

Demonstration of mobile PCR operation~Detects genes in about 30 minutes from water sampling~
Go!Foton, Inc. and Pacific Consultants Co., Ltd
Go!Foton and Pacific Consultants have been jointly developing applications for PicoGene, a portable PCR
device for the environmental DNA field.
With conventional methods, water samples must be brought back to the laboratory for analysis, and it takes
several days to a week before results are available. In contrast, by using a simple extraction and microfluidic
chip, and a portable PCR device named PicoGene, DNA detection results can be obtained in about 30 minutes onsite. It also allow the user to move on to the next place while PCR analysis is ongoing. This method isparticularly effective for screening target species in tributaries and streams with high DNA concentrations.
The results of the development of the process and the simplified extraction method, which can be completed inabout 30 minutes from water sampling to filtration, extraction, and PCR detection, have been summarized in apaper by Dr. Doi of Kyoto University.
The method using a microfiltration chip with a microfluidic channel, which was devised to further simplify
filtration and extraction, is also summarized in the paper.
On-site environmental DNA detection of species using ultrarapid mobile PCR,
DOI: 10.1111/1755-0998.13448
Filtration and extraction using microfiltration channel for measuring environmental DNA,
DOI: 10.1111/1755-0998.13657
In this demonstration, we show all of process from filtration, extraction, and PCR detection using actual sample
water. We plan to show you both the simple method and the microfluidic chip method, as well as have you try
them out for yourself.
Go!Foton has acquired PicoGene PCR1100 business from Nippon Sheet Glass Co., Ltd. as of 2023/04/01.
Go!Foton expand PCR business with PicoGene from now on.

24

Closing Ceremony
May 19th (Fri) 16:00 - 17:00 (Venue: Piazza Hall)
Is eDNA good enough? Implications for science and society
Mehrdad Hajibabaei, Ph.D., Associate Prof. (University of Guelph, Canada)

Our linkages to biodiversity impacts every aspect of our life and wellbeing. However, our ability to measure andstudy biodiversity has been limited because conventional methods are not scalable or feasible for many
applications. The need for tools and approaches for biodiversity analysis is at an all-time high given that we arefacing a “biodiversity crisis” triggered by climate change and other anthropogenic activities. Methods developed
based on environmental DNA (eDNA) are clearly making significant contributions to biodiversity analysis in awide range of settings backed by thousands of scientific papers in the last decade. However, these use cases are
somewhat limited to academic, artisanal and proof-of-concept cases. Real-world, systematic and on-going use of
eDNA tools in scientific initiatives or environmental assessment and monitoring programs has been somewhat
limited. In the absence of an absolute and precise measure of biodiversity in most settings, users have difficulty
verifying and validating data generated through eDNA studies as compared to traditional approaches. Coupledwith a lack of standardized and interoperable protocols and an ever quickly evolving technologies, these issues
have created barriers to widespread use of eDNA. While we need to address these concerns by directing
resources and strategic planning, it is also important to focus on the eDNA’s core premise and ask if eDNA isgood enough for the tasks at hand. I argue that eDNA tools, when properly applied, are currently one of the best
available options for rapid and accurate biodiversity analysis at the scale we need to better understand andmitigate the biodiversity crisis. The recent COVID-19 pandemic has taught us that solutions which are “goodenough” can be justified when the urgency of the task at hand requires rapid deployment of the best available
tools.

25

Company Exhibition
Promega K.K.
Bioengineering Lab. Co., Ltd.
Illumina K.K.
Civil Engineering & Eco-Technology Consultants Co., Ltd.
Go!Foton, Inc.
Environmental Research & Solutions Co., LTD.
QIAGEN K.K.

Sponsors
Pacific Consultants Co., LTD.
Illumina K.K.
Go!Foton, Inc.
Civil Engineering & Eco-Technology Consultants Co., Ltd.
KANSO TECHNOS CO., LTD.

The eDNA
Society International
Meeting 2023
The eDNA Society International Meeting 2023 Committee
Toshifumi Minamoto (The eDNA Society President / Kobe U.)
Hiroki Yamanaka (Ryukoku U.)
Kimiko Uchii (Osaka Ohtani U.)
Hideyuki Doi (Kyoto U.)
Akihide Kasai (Hokkaido U.)
Yuki Minegishi (U. Tokyo)
Teruhiko Takahara (Shimane U.)
Satoshi Yamamoto (NARO)
Noriyuki Koizumi (NARO)
Hiroaki Murakami (Tohoku U.)
Ryohei Nakao (Yamaguchi U.)
Keiko Muraoka (PWRI)
Masayuki Sakata (Hokkaido U.)
Jiro Okitsu (OYO Corp.)
Miho Matsumura (OYO Corp.)
Yuka Ikoma (Secretariat, The eDNA Soc.)
Yukiko Kobayashi (Secretariat, The eDNA Soc.)
Mari Norichi (Secretariat, The eDNA Soc.)

The eDNA Society International Meeting 2023 Program
Meeting
May 17th – 19th
Excursions May 20th – 21st
Piazza Omi 1-1-20, Nionohama, Otsu, Shiga, 520-0801, JAPANPublished:
Issued by:

May 1st, 2023
The eDNA Society
c/o Ryukoku University
1-5, Yokotani, Seta Oe-cho, Otsu, Shiga, 520-2194, Japane-mail: office@ednasociety.org
Homepage: http://ednasociety.org/

Copyright 2023 The eDNA Society, Japan All rights reserved（無断転載不可）