Anti-HB-EGF antibody, mouse monoclonal (4G10) (biotin)
Product code71-503

Size50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

ImmunogenPurified recombinant human HB-EGF ectodomain expressed in SF21 cell.

Isotype

Mouse IgG1κ

Reactivity

Human, but not with mouseSpecial notes

Epitope: EGF domain
Conjugation: biotin [biotin/IgG = 7.5]

Application

1. Western blotting (0.2~1 µg /ml) under non-reducing conditions
2. Immunoprecipitation (2 µg/ml)
3. Immunofluorescent staining (1 µg/ml)

BackgroundHeparin-binding epidermal growth factor-like growth factor (HB-EGF) issynthesized as a membrane-anchored precursor that is proteolytically cleaved
to release the soluble mature growth factor, HB-EGF (1, 2). The former functions
as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows
several forms in Western blotting with apparent molecular weights 19~27 kDa
due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF
activates EGFR and ErbB4 and promotes the development in many tissues. In
human ProHB-EGF is the cellular receptor for diphtheria toxin (3). Non-toxic
mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF
level is elevated in most ovarian cancer, CRM197 is being tested as an
anticancer drug (4). The hybridoma clone 4G10 was established andcharacterized in the laboratory of Prof. E. Mekada of Osaka University, who isa leading scientist in this field (3, 4).

Data Link

UniProtKB/Swiss-Prot Q99075 (HBEGF_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 71-503 Anti-HB-EGF antibody, mouse monoclonal (4G10) (biotin)( b( a( cFig.1

Identification of human HB-EGF by using anti-HB-EGF (clone 4G10)(a) Western blotting
Samples 1: Vero cell extract
Sample 2: Vero cells carrrying human HB-EGF expression vectorSample 3: Vero cells carrying mouse HB-EGF expression vector(b) Immunoprecipitation
Samples are the same as (a) except that the cell surface was biotinylated.
(c) Immuno-cytochemistry
Samples; (Vero-H) Vero cells carrying human HG-EGF expression vector, (Vero-mH) Vero cells
carrying mouse HB-EGF expression vector. Cells treated with antibody 4G10 were fixed with 4% PFA
and reacted with Cys3 conjugated 2nd antibody.
References: This antibody (non-biotinylated form) has been used in the following publictions.
1. Tang XH et al “The anti-tumor effect of cross-reacting material 197, an inhibitor of heparinbinding EGF-like growth factor, in human resistant ovarian cancer. Biochem Biophys ResCommun. 2012 Jun 15;422(4):676-80. PMID:22609777. IHC (human ovarian xenograph)
2. Hamaoka M et al. Anti-human HB-EGF monoclonal antibodies inhibiting ectodomain shedding ofHB-EGF and diphtheria toxin binding. J Biochem. 2010 Jul;148(1):55-69. PMID: 20332144. WB,IF, IP (human)
Related product71-501 Anti-HB-EGF antibody, mouse monoclonal (4G10)
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BioAcademia,Inc. Tel. 81-6-6877-2335

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01-515 Diphtheria toxin mutant CRM197
01-517 Diphtheria toxin

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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/