Anti-CD40 antibody, mouse monoclonal (5C3)
Product code72-030Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium

Immunogen

Recombinant extracellular domain of CD40

Isotype

Mouse IgG1κ

Reactivity

HumanSpecial notes

N/AApplication

1. Flow cytometry
2. Immunofluorescence staining and Immunocytochemistry (1/100)
3. Immunohistochemistry(acetone or zinc-fixed or frozen section; indirect

immuno-staining) (1/100)
4. Stimulation of proliferation of B cell and dendritic cells (20µg/ml).
Enhancement of proinflamentory cytokine production in human monocytes,
such as TNF-a, IL-6 and IL-8
Background

CD40 is a 45-50-kDa glycoprotein belonging to the tumor necrosis factor (TNF)
receptor superfamily. CD40 is specifically expressed on the surface of B cells andspecialized antigen-presenting cells such as dendritic cells and macrophages.
CD40 interacts with the CD40 ligand (CD154) which is found primarily on T
cells, playing a role in both humoral and cell-mediated immune responses.
Activation of CD40 on B cells by CD40 ligand causes B cell proliferation,differentiation, immunoglobulin isotype switching, germinal center formation,and stimulation of the humoral memory response. CD40 has been found tomediate a broad variety of immune and inflammatory responses. Within the cell,
the CD40 molecule acts as a transmembrane signal transducer that leads toactivation of intracellular kinases and transcription factors.

Data Link

UniProtKB P25942 (TNR5_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 72-030 Anti-CD40 antibody, mouse monoclonal (5C3)

Fig.1 Expression of CD40 on the cell suface of monocyte derived dendric cells as analyzed by Flow
Cytometry with anti-CD40 antibody (5C3).
Monocyte-derived dendric cells from healthy adult donor were stained with anti-hCD40 antibody(solid line) or with isotype control (broken line).

Fig.2 Immunofluorescent staining of CD40 in MCF7 cells with anti-CD40 antibody (5C3)
Cells were fixed in 4% paraformaldehyde and permeabilized in 0.25% Triton X-100.
Anti-CDCD40 was used at 1/100 dilution and as 2nd antibody, Alex 488 conjugated goat anti-mouse
IgG was used at 1/1,000 dilution. DNA was stained with DAPI.
Related Products:
72-031 anti-CD40 antibody (5C3), Biotin-conjugated.
72-032 anti-CD40 antibody (5C3), FITC-conjugated.
References: This antibody is used in ref.2 and 3.
1. Inui S et al (1990) “Identification of the intracytoplasmic region essential for signal transduction
through a B cell activation molecule, CD40.” Eur J Immunol 20: 1747-1753 PMID: 1698631 FC,
Stimulation of B cell prolification.
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2. Yasui T et al (2002) “Dissection of B cell differentiation during primary immune responses in micewith altered CD40 signals.” Int Immunol 14: 319-329 PMID: 11867568 FC
3. Ishida I et al (2003) “Involvement of CD100, a lymphocyte semaphoring, in the activation of thehuman immune system via CD72: implications for the regulation of immune and inflammatory
responses.” Int Immunol. 15: 1027-1034 PMID: 12882840

FC, Stimulation of B cell prolification.

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