Anti-Rad21 (S. pombe) antibody, rabbit serum
Product code63-139Size

50 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityRabbit antiserum

Immunogen

Recombinant full-size S. pombe Rad21 protein (His-tagged)

IsotypeRabbit IgG

Reactivity

S. pombe Rad21 protein.

Special notes

N/A

Application

1. Western blotting (1/1,000~1/4,000 dilution).
Not tested for other applications

Background

Rad21 protein (628 aa, 67.8 kDa) is a cleavable component of the cohesin
complex, involved in chromosome cohesion during cell cycle. The cohesin
complex is required for the cohesion of sister chromatids after DNA replication.
The cohesin complex apparently forms a large proteinaceous ring within which
sister chromatids can be trapped. At metaphase-anaphase transition, thisprotein is cleaved by Cut1 and dissociates from chromatin, allowing sister
chromatids to segregate. Also involved in the DNA double-strand-break (DSB)
repair system Hyperphosphorylated during S and G2 phases. Proteolytic
cleavage of a fraction of hyperphosphorylated form at the onset of anaphase may
be essential for the proper progression of anaphase and sister chromatid
separation. Belongs to the rad21 family, conserved to mammals.

Data Link

S. pombe Gene DB: rad21

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 2

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 63-139 Anti-Rad21 (S. pombe) antibody, rabbit serum

Fig.1

Identification of Rad21 protein in crude extract of S. pombe with anti-Rad21 antibody.

WT cells and rad21-9xPK cells grown in YS5S to 1x107, collected, washed and broken by glass beads.
Twenty ug proteins per lane were run on SDS-PAGE. First antibody at indicated dilutions in PBST
plus 2.5% skim milk was incubated at 4℃ overnight. Second antibody at 1/10,000 of anti-rabbit HRP
in PBST was incubated at room temperature for 1 h. Detected by ECL (X-ray film). Rad21 protein
migrated at the position of~110 kDa in SDS-PAGE although the molecular mass is 67.8 kDa (R.ef.3).
Multiple bands of Rad21 reflect multiple phosphorylation states.
References: This antibody was used in the following references.
1. Nagao K, Adachi Y, Yanagida M. Separase-mediated cleavage required for DNA repair. Nature.
(2004) 430:1044-8. pubmed/15329725 WB (S. pombe)
2. Nagao K, Yanagida M. Securin can have a separase cleavage site by substitution mutations in thedomain required for stabilization and inhibition of separase. Genes Cells. 2006 Mar;11(3):247-60.
PMID:16483313. WB (S. pombe)
3. Adach Y et al. Cut1/separase-dependent roles of multiple phosphorylation of fission yeast cohesion
subunit Rad21 in post-replicative damage repair and mitosis. Cell Cycle. (2008) 15:76576.pubmed/18239448. WB (S. pombe)
4. Murayama Y, Uhlmann F. Biochemical reconstitution of topological DNA binding by the cohesinring. Nature. 2014 Jan 16;505(7483):367-71. PMID: 24291789. WB (S. pombe)
2 / 2

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/