TECHNICAL DATA SHEET

Human IL-4, cct-premium
Human Interleukin-4, recombinant, cct-premium
Synonyms: IL4, BCGF, BCDF, B cell stimulating factor (BSF-1)
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENINGSize
5 µg

Order #
1515.950.005

25 µg

1515.950.025

50 µg

1515.950.050

100 µg1515.950.100

200 µg1515.950.200

500 µg1515.950.500

1 mg

1515.950.199

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant human Interleukin-4, cct-premium is a 14.9 kDa globular protein containing 130 amino acid residues.
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival andgene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiationof naïve CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretionof IL-4, IL-5, IL-6, and IL-13, which favour a humoral immune response. Another dominant function of IL-4 is theregulation of immunoglobin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells
has been associated with elevated IgE production and allergy.
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Biological Activity
SourcePurity
Endotoxin level
Stabilizer
BufferPhysical state≥ 8 x 106 IU/mg
E. Coli≥ 98 % (SDS-PAGE, silver stained)≤ 0.01ng/µg (≤ 0.1EU/µg)
None
PBS*Sterile filtered, lyophilized

Biological Activity
The ED50 of ≤ 0.12 ng/ml was determined by the dose-dependent stimulation of the proliferation of human TF-1cells. In direct comparison with the WHO standard (NIBSC 88/656) it corresponds to a specific activity of ≥ 8 x 106
IU/mg.

Reconstitution
We recommend a quick spin followed by reconstitution in water to a concentration of at least 0.1mg/ml. This solution
can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.

Stability
The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-4 is stable for atleast 3 months when stored in working aliquots with a carrier protein at -20°C. Please avoid repeated freeze-thaw
cycles.

Amino Acid Sequence
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGATAQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORTTel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEET
Purity and Biological Activity of Human IL-4, cct-premium

Fig. 1: SDS-PAGE analysis ofrecombinant human IL-4, cctpremium. Sample was loadedin 15% SDS-polyacrylamide
gel under reducing conditions
and stained with Silver staining.

Fig. 2: Proliferation assay withTF-1 cells. The cells werestimulated using recombinanthuman IL-4, cct-premium andthe WHO standard 88/656.
The cells were stimulated withincreasing amounts of the recombinant proteins. Values
are the means (±SD) of triplicate determinations and expressed as percentage of control.

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg