TECHNICAL DATA SHEET

Human GM-CSF, cct-premium (HSA)
Human Granulocyte Macrophage Colony Stimulating Factor, recombinant, carrier: HSA
Synonyms: CSF2, GMCSF
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENINGSize
10 µg

Order #
1450.951.010

50 µg1450.951.050

200 µg1450.951.200

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consistingof 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several
other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formationof granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated Tcells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune andinflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid,megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for andactivates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also beenreported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate andproliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including
osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF
has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface
receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to beheterodimers consisting of a GM-CSF-specific a chain and a common ß chain that is shared by the high-affinity
receptors for IL-3 and IL-5.
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SourcePurity
Endotoxin level
Stabilizer
BufferPhysical state

E. Coli≥ 98 % (SDS-PAGE, silver stained)≤ 0.01 ng/µg of protein (≤ 0.1EU/µg)
HSAPBS, pH 7.2*
Sterile filtered, lyophilized

Biological Activity
The ED50 of ≤ 0.1 ng/ml was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
This corresponds to a specific activity of ≥ 1.0 x 107 units/mg.

Reconstitution
We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of 0.1 mg/ml. Do
not vortex. This solution can then be diluted into other aqueous buffers and stored at 4°C for up to 1 week or at 20°C for future use.

Stability
The lyophilized GM-CSF, although stable at room temperature for 3 weeks, is best stored desiccated at -20°C.
Reconstituted GM-CSF should be stored in working aliquots at -20°C. For long term storage it is recommended toadd a carrier protein (0.1% HSA or BSA).

Amino Acid Sequence
APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSLTKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEETUsage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg