TECHNICAL DATA SHEET

Human FGF-10 / KGF-2, cct-premium
Human Fibroblast Growth Factor-10, Keratinocyte Growth Factor-2, recombinantSynonyms: FGF10, KGF2, KGF-2, FGFA.
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENING
Size
10 µg

Order #4735.950.010

25 µg4735.950.025

50 µg4735.950.050

100 µg4735.950.100

250 µg4735.950.250

500 µg4735.950.500

1 mg

4735.950.199

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant Human Fibroblast Growth Factor-10 (FGF-10 or KGF-2) is a 170 amino acid protein, with a molecularmass of 19,3 kDa. It is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family mostrelated to KGF/FGF-7. FGF family members possess extensive mitogenic and cell survival functions, and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair,tumor growth and invasion. FGF-10 is expressed during development and preferentially in adult lungs. It signals
through FGFR 2b.
•••••••Biological Activity
SourcePurityEndotoxin level
Stabilizer
BufferPhysical state≥ 2 x 106 units/mg
E. Coli≥ 95 % (SDS-PAGE, silver stained)≤ 0.01 ng/µg of protein (≤ 0.1EU/µg)
None
PBS (0,5x)*
Sterile filtered, lyophilized

Biological Activity
Assay 1: The ED50 of ≤ 0.5ng/ml was determined by the dose-dependent stimulation of thymidine uptake of BaF3
cells expressing FGFRs. It corresponds to a specific activity of ≥ 2.0 x 106 units/mg.
Assay 2: An ED50 of 0,5-1,0 ng/ml was determined by the FGF-10 induced proliferation of 4MBr-5 cells.

Reconstitution
We recommend a quick spin followed by reconstitution in water to a concentration of at least 50 µg/ml. Do notvortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working
aliquots should be at the highest practical concentration. For long term storage we recommend to add at least 0.1%
HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA.

Stability
The lyophilized protein is stable at room temperature for up to 1 month and at least until the lot specific expiry date
if kept below -18°C. Reconstituted FGF-10 should be stored in working aliquots at -20°C to -80°C if possible withcarrier protein. Please avoid repeated freeze-thaw cycles.

Amino Acid Sequence
MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY
SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYVALNGKGAPRR GQKTRRKNTS AHFLPMVVHS______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEETUsage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg