TECHNICAL DATA SHEET

Human EGF, cct-premium
Human Epidermal Growth Factor, recombinant, cct-premium
Synonyms: Epidermal growth factor, Urogastrone, URG
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENING
Size
100 µgOrder #
4530.950.100

500 µg4530.950.500

1 mg

4530.950.199

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant human Epidermal Growth Factor (EGF) is a 6.35 kDa globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds. EGF is a potent growth factor, which stimulates the proliferation ofvarious epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to beinvolved in wound healing. EGF signals trough a receptor known as c-erbB, which is a class I tyrosine kinase
receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation and colony formation of epidermal cells in culture.
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Biological Activity
SourcePurity
Endotoxin level
Stabilizer
BufferPhysical state≥ 4 x 105 IU/mg
E. Coli≥ 98 % (SDS-PAGE, HPLC)≤ 0.01 ng/µg of protein (≤ 0.1EU/µg)
None
PBS*
Sterile filtered (0.2 micron filter), lyophilized

Biological Activity
The ED50 of ≤ 2.5 ng/ml was determined by its ability to stimulate the proliferation of primary NHDF cells. It corresponds to a specific activity of ≥ 4.0 x 105 units/mg. This corresponds to an activity of ≥ 2.0 x 107 units/mg in amouse BALB/c 3T3 assay.

Reconstitution
We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of at least
100µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer - if possible - containing a carrier (example 5% Trehalose) and store in working
aliquots at -20°C to -80°C.

Stability
The lyophilized protein is stable at room temperature for up to 1 month, at 4°C for up to 6 months, and below -20°C
until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrierat -20°C to -80°C. Please avoid repeated freeze-thaw cycles.

Amino Acid Sequence
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEETSDS-PAGE and Biological Activity of Human EGF, cct-premium

Fig. 1: SDS-PAGE analysis ofrecombinant human EGF.
Sample was loaded in 18%
SDS-polyacrylamide gel under
reducing conditions andstained with Silver stain.

Fig. 2: Measurement of thedose-dependent stimulation ofcell proliferation in Normal Human Dermal Fibroblast(NHDF) cells by recombinanthuman EGF, cct-premium andWHO standard 91/530. Values
are the means (±SD) of triplicate determinations and expressed as percentage of control.

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbHOberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg