1P090!"#$%&'()*'+, -./ 012*34(1):56789:;< FAD = NADH * >?@AB–/CB-./ 012(DEFGH) I8JKLMNOPQRST UVWX*YZ[\9:]^_&'`abc9de^UVWXfghi<j=kl_UVmn#9opqrsi<&'bc*&'($%&')ft]^YZ[\fuvwxyz{f|}^]<1)~$%&'ft]<j=9de^_gh9d<WX*yklk_gh f=k]}9ky=k<~p_$%&'ft]^fuv}9_$%&'()*'9]^*)k3y<~_&'kl&'f. ¡¢£¤i<j=9d_¥¦§¨9©i<!"#*ª«¬­9]^®¯^]<2-6)~&''°h±+,'k²9³rk]}9_\´9µ¶^´·¸yE¹lº»i<~¼H½¾¿Àk$%&'()
<FAD (flavin adenine dinucleotide)=NADH (nicotinamide adenine dinucleotide)*>?@A
Bpq/CB9:;<&'-./012f\´_jÁq*)G*'+,-./0129
]^®¯~Â9ÃÄ9Å¸*Æ]¤ÇÈÉ¡Ê(ËÌGly=Íi)=5=*ÎÏ67ft]_Ðc*Å¸³r¸9]^®¯~ÑqÁÒÓfÔ=9_FAD=NADH*&'ft]!"#*Å¸ÕÖ*[\*¸f×e~FAD*)GØÙfFig. 1A9Úi~RÛÜT >?@ABpqÝÞBßà*&'-./012*\´_LáâãUxft]~>?@ABäå£æ>ç.èé¡ê¡(~81 MHz)pq*ëì'f29);_íw2îâ9¬ï+,'9_ðwLáâ'fãUñò<}*ó¡A'=~+,'9de^UôWXpq*&'=ó¡A'fBBOÒõ9ö÷_UôLáâ'f>øÊå¡=)'ù9de^)'_'Gúû®ë~üýþÿ û!250 fs<~ÝÞBpq/CBßà*&'"#$%*\´_ & 'G'ûxft]~®ëù=^(Êää)£*Ê+é
¡A#,-'FGºî.ft]_üýþÿ û!60 ps<~WX'/01 mm*>É¡ÈÊ9^1ªñò^]<~WX2!1310–4 mol dm–3<~RÒÓTFig. 1B 9Ç£456U785(PBS)89:;< FAD *9::d;&'2<1A
ÊfÚi~FAD =à99:f>?_='+,9:;<$%&'*@()=ABqÁ^]<~Fig. 2A 9 FAD *>?@ABpqÝÞBßà
*&'-./012*5CGly ÎÏµ³r¸fÚi~(A)+,'=&'*â0_410 nm = 580 nm <~ÎÏµ9ppDqE_ÝÞBßà*F]&'fAdenine>()=/CBßà*0]&'f>()*E¹l 2 ()9);<j=y¹<~FAD GHÕIsoalloxazine
Ö9:]^_èIJ£K)=.LèÉMæ¢£K)
yNÁ Open form =_jÁqyOP Stackedform * 2 *ÕÖ*QRÕÖ=^rsi<~Open(B)form /CB*&'f>?_!"[\9)k&'fSB<*9©_Stacked form *&'_+,ñÁ.LèÉMæ¢£K)=èIJ£K)=*)G#FGTU9d<V'*}9_! 10
Fig. 1. (A) Chemical structure of FAD. (B)Absorption and fluorescence spectra of FAD in7,8)~WX[ps =ÃÄ9Flk<=ABqÁ^]<PBS. Excitation wavelength was 410 nm.

!"#$%&'()*+,-./012Stacked form ,345678&-.9:,/;<=!>$%&?@ABC?DEF&G'()*+,/0HI<JK>LMN< Fig. 2B 7OBG
FAD ,PQR8S-.TUVWX,YZ[2\]^78_$`a>Ab_LGcLde
f78&?ghi#$%& 1 ps jk,lm7n%oI,pq[2580 nm ,-.<r%&C?78_$sI7t"uB&C?DEF& 7,8)GFig. 2B bivibA8w72Gly ,x:yz
7{%2'(),/0oI,-.|}D~uA&C?Db&G,@E[ 10 ps ,-.
|}E&,7>2Gly D 60 %,?F7[2-.|}[ 30 ps ?yz>$%&GC,M
N[2Stacked form ,I43456n:D2Gly ,yz7{%/;B&C?<O>$%&G
Stacked form , iDI434567R%$E&C?<O>$%&GcL),-.|},oI7B&'(),oID2Gly ,yz7%/;>$%&(Fig. 2A)G),-.|}< Open form ,D2Gly 7%yzB&C?<O>$%&GFig.3A 72FAD ,-.TUVWX,Gly \]^<OBGGly DyzB&7#$-.9:DyzB&C?Db&G-./0,!bi12Open form 7B&),-.|}D2Gly ,yz7_$~uA_$%&(Fig. 3B)GGly ,yz78& FAD ,-.9:,yz[2Open form ,(Fig. 2A)?-.|}(Fig. 3B),yz7B&C?Db&G, ¡¢£¤,J¥7R%$2,¦Z§Dlm7E
&G¨©=!"#L FAD ,-.|},–Gly \]^[2FAD ,-.|}<r%$2,¦Z§<=!EF&ª«<O>$%&G¬­E[2®¯A°X(±X<r%L FAD ,-.²³´VTR8S NADH ,-.|},¦7%$1µ¶B&G
1) D. Chorvat, Jr. et al., Laser Phys. Lett. 6 (2009) 175. 2) !"#$%, &' ( ) 58 (2009) 473 (*+). 3) T. Ito et al.,Photochem. Photobiol. Sci. 8 (2009) 763. 4) N. Ohta et al., Proc. of SPIE 7190 (2009) 71900R. 5) T. Nakabayashi et al.,Photochem. Photobiol. Sci. 7 (2008) 668. 6) T. Nakabayashi et al., Photochem. Photobiol. Sci. 7 (2008) 671. 7) H.
Chosrowjan et al., Chem. Phys. Lett. 378 (2003) 354. 8) Y.-T. Kao et al., J. Am. Chem. Soc. 130 (2008) 13132.

(A)(B)

Fig. 2. (A) Normalized femtosecond time-resolved
fluorescence decays of FAD in water/glycerol solution.
Excitation and probe wavelengths were 410 and 580
nm, respectively. Glycerol percent weight is shownon the right of the figure. (B) Extraction of thepicoseond decays of FAD of (A) with removal of thelong-time components.

(A)(B)

Fig. 3. Fluorescence spectra (A) and normalized
nanosecond time-resolved fluorescence decays(B) of FAD in water/glycerol solution.
Excitation and probe wavelengths were 410 and
580 nm, respectively. Glycerol percent weightis shown on the right of the figure.