TECHNICAL DATA SHEET

Human GM-CSF, cct-premium
Human Granulocyte Macrophage Colony Stimulating Factor, recombinantSynonyms: CSF2, GMCSF
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENINGSize
25 µg

Order #
1450.950.025

50 µg1450.950.050

100 µg1450.950.100

200 µg1450.950.200

500 µg1450.950.500

1 mg

1450.950.199

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consistingof 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several
other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formationof granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated Tcells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune andinflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid,megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for andactivates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also beenreported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate andproliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has
no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface
receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to beheterodimers consisting of a GM-CSF-specific a chain and a common ß chain that is shared by the high-affinity
receptors for IL-3 and IL-5.
••
•
•
•
•
•

Biological Activity
SourcePurityEndotoxin level
Stabilizer
BufferPhysical state≥ 1 x 107 IU/mg
E. Coli≥ 98 % (SDS-PAGE, silver stained)≤ 0.01 ng/µg of protein (≤ 0.1EU/µg)
None
PBS, pH 7.2*
Sterile filtered, lyophilized

Biological Activity
The ED50 of ≤ 0.1 ng/ml was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
This corresponds to a specific activity of ≥ 1.0 x 107 units/mg.

Reconstitution
We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of 0.1 mg/ml. Do
not vortex. This solution can then be diluted into other aqueous buffers and stored at 4°C for up to 1 week or at 20°C for future use.

StabilityThe lyophilized protein is stable at room temperature for up to 3 weeks and below -20°C until expiry date. Reconstituted human GM-CSF should be stored in working aliquots at -20°C. Please avoid repeated freeze-thaw cycles.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEETAmino Acid Sequence
APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSLTKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
SDS-PAGE and Biological Activity of Human GM-CSF, cct-premium

Fig. 1: SDS-PAGE analysis ofrecombinant Human GM-CSF,cct-premium. Sample was
loaded in 15% SDS-polyacrylamide gel under reducing
condition and stained with Silver stain.

Fig. 2: Dose-dependent stimulation of cell proliferation in TF1 cells by recombinant HumanGM-CSF, cct-premium and theWHO standard 88/646. Values
are the means (±SD) of triplicate determinations and expressed as percentage of control.

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.deTECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg