TECHNICAL DATA SHEET

Human FGF-basic, cct-premium, FGF-2
Human Fibroblast Growth Factor-basic, recombinant, cct-premium
Synonyms: FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2,Prostatropin.
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENING
Size
10 µg

Order #
1370.950.010

50 µg1370.950.050

100 µg1370.950.100

200 µg1370.950.200

500 µg1370.950.500

1 mg

1370.950.199

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

DescriptionRecombinant human Fibroblast Growth Factor-basic (hFGF-b) is a 16.5 kDa protein containing 153 amino acidresidues. Interestingly, hFGF-b contains no hydrophobic leader sequence previously thought to be required for cellsecretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 aminoacid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues.
hFGF-b is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells includingmesenchymal, neuroectodermal and endothelial cells. It also exerts a potent angiogenic activity in vivo. Acidic andbasic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher
affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. In general FGF’s induce the proliferative responsein cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling timeby shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogenactivator by endothelial cells. One of the most potentially significant applications of FGF-b is related to its reported
ability to induce angiogenesis.
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Biological Activity
SourcePurityEndotoxin level
Stabilizer
BufferPhysical state≥ 1 x 107 IU/mg
E. Coli≥ 98 % (SDS-PAGE, silver stained)≤ 0.01 ng/µg of protein (≤ 0.1EU/µg)
None
PBS*Sterile filtered, lyophilized

Biological Activity
The ED50 of ≤ 0.1 ng/ml was determined by stimulation of cell proliferation in HUVEC (human umbilical vein endothelial cells) in direct Comparison with WHO standard #90/712. It corresponds to a specific activity of ≥ 1.0 x 107
IU/mg.

Reconstitution
We recommend a quick spin followed by reconstitution in water to a concentration at least 0.05 mg/ml. Do notvortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working
aliquots should be at the highest practical concentration. For long term storage we recommend to add at least 0.1%
HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz).

Stability
Lyophilized samples are stable until expiry date at -20°C to -80°C. FGF-basic can be stored in high-salt buffer (PBS,1M NaCl) at 4°C for 2-4 weeks. Please avoid repeated freeze-thaw cycles.
______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERINGTel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORTTel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEETAmino Acid Sequence
AGSITTLPAL PEDGGSGAFP PGHFKDPKRL YCKNGGFFLR IHPDGRVDGV REKSDPHIKL QLQAEERGVVSIKGVCANRY LAMKEDGRLL ASKCVTDECF FFERLESNNY NTYRSRKYTS WYVALKRTGQ YKLGSKTGPG
QKAILFLPMS AKSSDS-PAGE and Biological Activity of FGF-basic, cct-premium

Fig. 1: SDS-PAGE analysis ofrecombinant human FGFbasic, cct-premium. Samples
were loaded in 15% SDS-polyacrylamide gel under reducing
and non-reducing conditionand stained with Coomassieblue.

Fig. 2: FGF-basic, cct-premium induced proliferation ofprimary human umbilical vein
endothelial cells (HUVEC).
The cells were stimulated using recombinant human FGF-2
and the WHO standard
90/712. Values are the means(±SD) of triplicate determinations and expressed as percentage of control.

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg