TECHNICAL DATA SHEET

Human TGF-beta1 (glycosylated, HEK), cct-premium
Human Transforming Growth Factor-beta1, recombinantSynonyms: Transforming Growth Factor-ß1, Differentiation inhibiting factor, Cartilage-inducing factor.
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENING
Size
10 µg

Order #
1934.955.010

50 µg

1934.955.050

100 µg1934.955.100

500 µg1934.955.500

1 mg

1934.955.199

10 mg

1934.955.109

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant human Transforming Growth Factor-beta 1 (TGF-b1) is a 25.0 kDa protein composed of two identical
112 amino acid polypeptide chains linked by a single disulfide bond. The three mammalian isoforms of TGF-beta,
TGF-beta1,-beta2, -beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis,
tissue remodelling and wound healing. They are secreted predominantly as latent complex which are stored at thecell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex
involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as
thrombospondin-1. TGF-beta1 is the most abundant isoform secreted by almost every cell type. It was originallyidentified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in theformation of skin tumors.
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Biological Activity
SourcePurity
Endotoxin levelStabilizer
BufferPhysical state≥ 2 x 107units/mg
HEK≥ 98 % (SDS-PAGE, HPLC)≤ 0.01ng/µg (≤ 0.1EU/µg)
None
TFA (0.1%)*
Sterile filtered, lyophilized

Biological Activity
The ED50 of ≤ 0.05 ng/ml was determined by TGF-beta 1’s ability to inhibit the mouse IL-4-dependent proliferationof mouse HT-2 cells. It corresponds to a specific activity of ≥ 2 x 107 units/mg.

Reconstitution
We recommend a quick spin followed by reconstitution in sterile Citric Acid (10mM; pH 3.0) to a concentration of0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it isrecommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working
aliquots at -20°C to -80°C.

Stability
The lyophilized protein is stable at room temperature for up to 1 month, at 4°C for up to 6 months, and below -20°C
until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrier
protein at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEET
Amino Acid Sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNPGASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORTTel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg