TECHNICAL DATA SHEET

Human IL-3, cct-premium
Human Interleukin-3, recombinant, cct-premium
Synonyms: IL3, IL-3, MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor.
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENING
Size
10 µg

Order #
1509.950.010

50 µg1509.950.050

100 µg1509.950.100

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant human Interleukin-3 (IL-3) is a 15.0 kDa globular protein containing 134 amino acid residues. It is ahematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cellsof the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced
by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles.
Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
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Biological Activity
SourcePurity
Endotoxin level
Stabilizer
BufferPhysical state≥ 1 x 107 IU/mg
E. Coli≥ 98% (SDS-PAGE, silver stained)≤ 0.01ng/µg (≤ 0.1EU/µg)
None
PBS*
Sterile filtered (0.2 micron filter), lyophilized

Biological Activity
The ED50 of ≤ 0.1 ng/ml was determined by the dose dependent stimulation of the proliferation of TF-1 cells. It
corresponds to a specific activity of ≥ 1 x 107 units/mg.

Reconstitution
We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration not less than 100µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended
to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C
to -80°C.

Stability
The lyophilized protein is stable at room temperature for up to 1 month, at 4°C for up to 6 months, and at -20°C
to -80°C until expiry date. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C
to -80°C. Please avoid repeated freeze-thaw cycles.

Amino Acid Sequence
MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQNASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORTTel.: +49 40 43208448-11support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEET
Purity and Biological Activity of recombinant human IL-3, cct-premium (E. coli)

Fig. 1: SDS-PAGE analysis ofrecombinant human IL-3,cctpremium. Samples wereloaded in 15% SDS-polyacrylamide gel under reducing
conditions and visualized with
Silver staining.

Fig. 2: Proliferation assay with
TF1 cells. The cells were stimulated using recombinant human IL-3, cct-premium and theWHO standard 91/510.

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg