TECHNICAL DATA SHEET

Human HGF, cct-premium (glycosylated, InCs)
Human Hepatocyte Growth factor, cct-premium, recombinantSynonyms: HGF, SF, HGFB, HPTA, F-TCF, DFNB39, Hepatocyte growth factor, Scatter factor, rh HGF
PLEASE NOTE: ALWAYS CENTRIFUGE VIAL BEFORE OPENING
Size
25 µg

Order #
2791.952.025

100 µg2791.952.100

500 µg2791.952.500

1 mg

2791.952.199

Lot #Expiry Date

Please enquire for bulk quantities and other vial sizes

Description
Recombinant human Hepatocyte Growth Factor (HGF) is a 78.0 kDa disulfide-linked heterodimeric protein. HGF isa multifunctional polypeptide that acts in a paracrine fashion on a wide variety of cell types. Human HGF, also know
as scatter factor, is a pleiotrophic cytokine that shows homology to the enzymes of the blood coagulation cascade.
Hepatocytes have to be primed (e.g. TNF and IL-6) before they can fully respond to HGF. Recent studies suggestthat HGF synergizes with FGF-basic in the induction of angiogenesis.
•••••

SourcePurity
Endotoxin levelBuffer
Physical state

Insect cells≥ 95 % (SDS-PAGE, silver stained)≤ 0.01ng/µg (≤ 1EU/µg)
Acetic Acid (50mM)*
Sterile filtered, lyophilized

Biological Activity
The ED50 of ≤ 3 ng/ml was determined by scattering activity in MDCK cells in direct comparison with WHO Standard
96/564. It corresponds to a specific activity of ≥ 3.3x105 units/mg.

Reconstitution
The lyophilized HGF, cct-premium is best soluble in 50mM acetic acid. It should be reconstituted to a concentrationof 100µg/ml. We recommend that further dilutions should be made into buffer containing carrier protein (e.g. HSA
ultra pure, 2835.958.xyz) or medium containing serum.

Stability
The lyophilized protein, though stable at room temperature for at least 3 weeks, is best stored desiccated below 18°C. Reconstituted HGF, cct-premium should be stored in working aliquots at -20°C to -70°C. Please avoid
repeated freeze-thaw cycles.

Amino Acid Sequencealpha chain:
QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF
NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKDLQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPHRHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGEGYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIPNCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPWCYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg

TECHNICAL DATA SHEET

beta chain:
VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEKCKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR
VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIPNRPGIFVRVA YYAKWIHKII LTYKVPQS
SDS-PAGE and Biological Activity of Human HGF, cct-premium (glycosylated, InCs)

Fig 1: SDS-PAGE analysis of recombinant human HGF, cct-premium derived
from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under
non-reducing conditions and stained with silver stain.

Fig 2: Measurementof scattering activity
in MDCK cells by recombinant human
HGF, cct-premiumand the WHO standard 96/564. Values
are the means (±SD)
of triplicate determinations and expressed as percentage ofcontrol.

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
______________________*The Buffer may vary depending on the Lot #. Please contact our technical support if you have specific requirements.
ORDERING
Tel.: +49 40 43208448-0
order@active-bioscience.dewww.active-bioscience.de

TECHNICAL SUPPORT
Tel.: +49 40 43208448-11
support@active-bioscience.de

Active Bioscience GmbH
Oberaltenallee 8D-22081 Hamburg
HRB 98170 Amtsgericht Hamburg