accelerating progress
is in our genes

Now compatible withnon-FFPE specimens
IQFISH Fast Hybridization Buffer works
with FFPE, blood and bone marrowspecimens, delivering fast hybridizationand high quality results. Image 5 (right)
is a PML-RARA dual fusion probehybridized to cultured bone marrowcells for 1.5hrs using IQFISH FastHybridization Buffer.

Image 5. A PML-RARA dual fusion probe hybridized to bone marrow cells for 1.5 hours
using IQFISH Fast Hybridization Buffer.

Ordering Information
Part Number

Product Description

IQFISH FastHybridization BufferHigh quality, half day
sample-to-result for FISH tests

VolumeG9415A

IQFISH Fast Hybridization Buffer 200

200 µL/vial (20 tests)

G9416AIQFISH Fast Hybridization Buffer 200x6

6x200 µL/vial; 6 vials

For Laboratory Use Only.

• Fast Results• High Quality Signal• Compatible with FFPE andblood/bone marrow samples

To learn more, scan the QR code or visit
www.agilent.com/genomics/surefish© Agilent Technologies, Inc. 2014
Printed in USA, May 16, 2014
5991-4294EN						See Deeper. Reach Further.

| Genomics

See Deeper. Reach Further.

| Genomicsbases

IQ bufferdiminishes base stacking

Image 1. Minimized base pairing interference enables fast binding between
FISH probe and specimen DNA to enable 1-2 hour hybridization.

IQFISH hybridization
enables visible signal
instantly
As shown in Image 3 (left) IQFISH
hybridization enables visible signal
instantly. Shown is a Her2/CEN-17
probe on FFPE breast carcinoma tissue.
Hybridization was stopped by stringent
wash buffer, 2 minutes and 15 minutes
after co-denaturation.

Image 3. Comparison of formamide-based and IQFISH buffers. A Her2/CEN-17 probe on FFPE breast carcinoma tissue 2 minutes and 15 minutes
hybridization after co-denaturation. Samples visualized on an epi-fluorescent
microscope.

Fast Results.
IQFISH enables 1-2 hourhybridization for quick
sample-to-reporting for
FISH testing
IQFISH Fast Hybridization Buffer’s
1-2 hour hybridization enables a half
day sample-to-result workflow. Thisworkflow offers operational excellence,
great average turnaround time, same
day repeat testing, and Friday testing
without weekend staffing. (Image 2)

15 minFormamide Based Hyb Buffer

Standard formamide based FISHhybridization buffer lowers melting
temperature by attacking hydrogenbonds and interfering DNA basepairing. The interference slows downbase pairing between FISH probeand specimen DNA. IQFISH buffer
destabilizes DNA helix by diminishinghydrophobic stacking of bases.
Minimized base pairing interference
enables fast binding between FISH
probe and specimen DNA. (Image 1)

Formamideinterferes with base pairing

2 min

H

IQFISH Fast Hyb Buffer

How IQFISH buffer
enables fast hybridization

Hydrogenbonds

High Quality Signal
IQ FISH fast hybridization buffer enables
fast hybridization with minimal loss insignal quality.

Image 2. Comparison between formamide and IQFISH buffer based workflows.
Image 4A: ALK IQFISH 1.5hr hyb Image 4B: RET IQFISH 1.5hr hyb

Image 4A and 4B. 1.5 hour hybridization with IQFISH fast hybridization buffer
on FFPE lung adenocarcinoma, using an ALK and RET break-apart FISH probes.

Optimal signal strength is reached after
2 hour hybridization. Image 4A and B(left) shows 1.5hr hybridization withIQFISH fast hybridization buffer on FFPElung adenocarcinoma, using an ALK andRET break-apart FISH probes, followedby visualization on an epi-fluorescent
microscope.