(57)【特許請求の範囲】
【請求項１】
配列番号２５８のアミノ酸配列と少なくとも９０％の同一性を有するアミノ酸配列を含み、ストレプトコッカス・ニュモニエに免疫応答を誘発可能な単離ポリペプチド。
【請求項２】
配列番号２５８のアミノ酸配列と少なくとも９５％の同一性を有するアミノ酸配列を含み、ストレプトコッカス・ニュモニエに免疫応答を誘発可能な請求項１記載の単離ポリペプチド。
【請求項３】
配列番号２５８のアミノ酸配列を含む請求項２記載の単離ポリペプチド。
【請求項４】
配列番号８の位置４９７−８３８のアミノ酸配列と少なくとも９５％の同一性を有するアミノ酸配列からなり、ストレプトコッカス・ニュモニエに免疫応答を誘発可能な単離ポリペプチドであって、サッカリドに融合している該単離ポリペプチドと、医薬的に許容性のキャリヤー又は希釈剤を含むワクチン組成物。
【請求項５】
単離ポリペプチドが、配列番号８の位置４９７−８３８のアミノ酸配列からなる請求項４記載のワクチン組成物。
【請求項６】
サッカリドに融合した請求項１〜３のいずれか1項記載の単離ポリペプチド。
【請求項７】
請求項１〜３のいずれか1項記載のポリペプチドをコードする単離ポリヌクレオチド。
【請求項８】
請求項７記載のポリヌクレオチドに相補的な単離ポリヌクレオチド。
【請求項９】
請求項８記載のポリヌクレオチドを含む発現ベクターであって、該ポリヌクレオチドが発現制御領域に操作的に連結されている発現ベクター。
【請求項１０】
請求項９記載の発現ベクターを含む宿主細胞。
【請求項１１】
請求項１〜３のいずれか1項記載のポリペプチドの製造方法であって、該ポリペプチドの発現に適する条件下、請求項１０記載の宿主細胞を培養する工程を含む、該ポリペプチドの製造方法。
【請求項１２】
さらに、該宿主細胞培養物から該ポリペプチドを単離する工程を含む請求項１１記載の方法。
【請求項１３】
請求項１〜３のいずれか１項記載のポリペプチドと、医薬的に許容性のキャリヤー、又は希釈剤を含むワクチン組成物。
【請求項１４】
さらに医薬的に許容性のアジュバントを含む請求項４、５及び１３のいずれか１項記載のワクチン組成物。
【請求項１５】
ストレプトコッカス感受性の個体のストレプトコッカス感染の予防的又は治療的処置用のワクチン組成物又は医薬組成物の製造のための、請求項１〜３のいずれか1項記載のポリペプチドの使用。
【発明の詳細な説明】
【０００１】
発明の分野
本発明は、抗原、エピトープ及びこのエピトープに向けられる抗体に係り、さらに詳細には、ストレプトコッカス感染の予防、診断又は治療に有用なストレプトコッカス・ニュモニエ病原のポリペプチド抗原に関する。
【０００２】
発明の背景
S.ニュモニエは、人、とりわけ乳児、高齢者及び免疫無防備状態人における病気の重要な物質である。それは、世界中で高い罹患率及び死亡率の菌血症／敗血症、肺炎、髄膜炎のような浸潤性の病気の患者から、頻繁に単離される細菌である。適切な抗生物質による治療によってさえ、未だに肺炎球菌感染の多くは死に至る。抗細菌薬の発展によって肺炎球菌性病気による全体的な死亡率は減少してきたが、抵抗性のある肺炎球菌生体の存在が今日の世界の主要な問題になってきた。有効な肺炎球菌ワクチンが、S.ニュモニエ病に関する罹患率及び死亡率に大きな影響力を与えることができた。このようなワクチンは、乳児及び幼児の中耳炎の阻止にも有用だろう。
【０００３】
肺炎球菌ワクチンを開発するための努力は、一般的に肺炎球菌莢膜多糖体に対する免疫応答を生じさせることに集中している。80種より多くの肺炎球菌莢膜血清型が、抗原性の相異に基づいて同定されている。最も頻繁に病気を引き起こした23種の肺炎球菌莢膜多糖体を含め、現在入手可能な肺炎球菌ワクチンは、主にいくつかの莢膜多糖体の不十分な免疫原性、血清型の多様性及び血清型の経時的、地理的領域及び年齢群分布の相異に関連する重大な欠点がある。特に、すべての血清型に対して幼児を保護するために現在開発している現存のワクチン及び莢膜接合ワクチンの失敗が、他のS.ニュモニエ成分の評価を刺激している。莢膜多糖体の免疫原性は改良できるが、未だ血清型特異性が多糖体ベースワクチンの主要な限界を表している。抗原性的に保存された免疫原性肺炎球菌タンパク質抗原それ自体又は付加成分と組み合わせた使用は、タンパク質ベース肺炎球菌ワクチンの可能性を提供する。
【０００４】
発明の名称“ストレプトコッカス・ニュモニエ抗原及びワクチン”で、1998年５月７日に公開されたPCT WO 98/18930は、抗原性であると特許請求している特定のポリペプチドについて述べている。しかし、これらポリペプチドの生物活性は何ら報告されていない。同様に、必要な種の共通ワクチン候補である配列保存も何ら報告されていない。
PCT WO 00/39299は、ポリペプチド及びこれらポリペプチドをコードするポリヌクレオチドについて述べている。PCT WO 00/39299は、BVH-3及びBVH-11と呼ばれるポリペプチドが、肺炎球菌による致命的な実験的感染を保護することを実証している。
従って、ストレプトコッカス感染の予防、診断及び／又は治療用の成分として使用可能なストレプトコッカス抗原について、満たされていない要望が残っている。
【０００５】
発明の概要
以下から選択されるポリヌクレオチドを含む単離ポリヌクレオチド；
（ａ）表Ａ、Ｂ、Ｄ、Ｅ又はＨから選択される第２ポリペプチドと少なくとも70％の同一性を有するポリペプチドをコードするポリヌクレオチド；
（ｂ）表Ａ、Ｂ、Ｄ、Ｅ又はＨから選択される第２ポリペプチドと少なくとも95％の同一性を有するポリペプチドをコードするポリヌクレオチド；
（ｃ）表Ａ、Ｂ、Ｄ、Ｅ又はＨから選択されるアミノ配列を有するポリペプチド；又はその断片、類似体若しくは誘導体をコードするポリヌクレオチド；
（ｄ）表Ａ、Ｂ、Ｄ、Ｅ又はＨから選択されるポリペプチドをコードするポリヌクレオチド；
（ｅ）表Ａ、Ｂ、Ｄ、Ｅ又はＨから選択される配列を有するポリペプチドに対して結合特異性を有する抗体を産生可能なポリペプチドをコードするポリヌクレオチド；
（ｆ）表Ａ、Ｂ、Ｄ、Ｅ又はＨから選択されるポリペプチドのエピトープ保持部をコードするポリヌクレオチド；及び
（ｇ）（ａ）、（ｂ）、（ｃ）、（ｄ）、（ｅ）又は（ｆ）のポリヌクレオチドに相補的なポリヌクレオチド。
【０００６】
他の局面では、本発明のポリヌクレオチドでコードされた新規なポリペプチド、医薬品又はワクチン成分、発現制御領域に操作的に連結された本発明のポリヌクレオチドを含むベクター、並びに前記ベクターで形質移入された宿主細胞及び前記宿主細胞を発現に適した条件下で培養する工程を含むポリペプチドの製造方法が提供される。
【０００７】
発明の詳細な説明
BVH-3及びBVH-11ポリペプチドの部分は内部にあることが分かった。他の部分は、病気を引き起こす被包s.ニュモニエ菌株のような重要な菌株には存在しなかった。内部でない部分を含むポリペプチドがあると有利だろう。ポリペプチドの大部分が内部にあると、これら部分は細菌上で露出されない。しかし、これら部分は、組換えポリペプチド内で非常に免疫原性であり、かつ感染に対する保護を与えないだろう。ほとんどの菌株に存在する部分を含むポリペプチドがあっても有利だろう。
本発明は、特異的な免疫応答を得るため、望ましくない部分が欠失及び／又は修飾されているポリペプチドに関する。
本発明によって、保護ドメインを含むポリペプチド又はこのようなポリペプチドをコードするポリヌクレオチドも提供される。
【０００８】
驚くべきことに、ポリペプチドの望ましくない部分が欠失又は修飾されると、該ポリペプチドは望ましい生物特性を有する。このことは、特許出願PCT WO 98/18930で、これら部分のいくらかがエピトープ保持部であるとして記述されたという事実を考えると驚くべきことである。PCT WO 00/37105のような他の公報では、ヒスチジン三つ組残基及び二重コイル領域として同定された部分が重要であると述べられた。本発明者らは、特定部分が欠失及び／又は修飾されたポリペプチドBVH-3及BVH-11の変異体及びこれらポリペプチドびキメラが生物学的特性を有し、かつ特異的な免疫応答を発生させることを見出した。
【０００９】
一局面によって、本発明は、本出願、表及び図面に開示されているような配列を含む第２ポリペプチドと少なくとも70％の同一性を有するポリペプチドをコードする単離ポリヌクレオチドを提供する。
本発明の一局面により、以下から選択されるポリヌクレオチドを含む単離ポリヌクレオチドが提供される；
（ａ）表Ｂ、Ｅ又はＨから選択される第２ポリペプチドと少なくとも70％の同一性を有するポリペプチドをコードするポリヌクレオチド；
（ｂ）表Ｂ、Ｅ又はＨから選択される第２ポリペプチドと少なくとも95％の同一性を有するポリペプチドをコードするポリヌクレオチド；
（ｃ）表Ｂ、Ｅ又はＨから選択されるアミノ配列を有するポリペプチド又はその断片、類似体若しくは誘導体をコードするポリヌクレオチド；
（ｄ）表Ｂ、Ｅ又はＨから選択されるポリペプチドをコードするポリヌクレオチド；
（ｅ）表Ｂ、Ｅ又はＨから選択される配列を有するポリペプチドに対して結合特異性を有する抗体を産生可能なポリペプチドをコードするポリヌクレオチド；
（ｆ）表Ｂ、Ｅ又はＨから選択されるポリペプチドのエピトープ保持部をコードするポリヌクレオチド；及び
（ｇ）（ａ）、（ｂ）、（ｃ）、（ｄ）、（ｅ）又は（ｆ）のポリヌクレオチドに相補的なポリヌクレオチド。
【００１０】
一局面によって、本発明は、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも70％の同一性を有するポリペプチド、又はその断片、類似体若しくは誘導体をコードする単離ポリヌクレオチドを提供する。
一局面によって、本発明は、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも95％の同一性を有するポリペプチド又はその断片、類似体若しくは誘導体をコードする単離ポリヌクレオチドを提供する。
一局面により、本発明は、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択されるアミノ酸配列を特徴とするポリペプチド、又はその断片、類似体若しくは誘導体に関する。
【００１１】
一局面によって、本発明は、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも70％の同一性を有するポリペプチドをコードする単離ポリヌクレオチドを提供する。
一局面によって、本発明は、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも95％の同一性を有するポリペプチドをコードする単離ポリヌクレオチドを提供する。
一局面により、本発明は、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択されるアミノ酸配列を特徴とするポリペプチドに関する。
【００１２】
一局面によって、本発明は、表Ｂ、Ｅ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも70％の同一性を有するポリペプチド又はその断片、類似体若しくは誘導体をコードする単離ポリヌクレオチドを提供する。
一局面によって、本発明は、表Ｂ、Ｅ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも95％の同一性を有するポリペプチド又はその断片、類似体若しくは誘導体をコードする単離ポリヌクレオチドを提供する。
一局面により、本発明は、表Ｂ、Ｅ若しくはＨから選択されるアミノ酸配列を特徴とするポリペプチド又はその断片、類似体若しくは誘導体に関する。
【００１３】
一局面により、本発明は、表Ｂ、Ｅ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも70％の同一性を有するポリペプチドをコードする単離ポリヌクレオチドを提供する。
一局面により、本発明は、表Ｂ、Ｅ若しくはＨから選択される配列を含む第２ポリペプチドと少なくとも95％の同一性を有するポリペプチドをコードする単離ポリヌクレオチドを提供する。
一局面により、本発明は、表Ｂ、Ｅ若しくはＨから選択されるアミノ酸配列を特徴とするポリペプチドに関する。
【００１４】
本発明により、ポリペプチド及びキメラポリペプチドをコードするすべてのヌクレオチドは、本発明の範囲内である。
さらなる実施形態では、本発明のポリペプチド又はキメラポリペプチドは抗原性である。
さらなる実施形態では、本発明のポリペプチド又はキメラポリペプチドは免疫原性である。
さらなる実施形態では、本発明のポリペプチド又はキメラポリペプチドは個体内で免疫応答を誘発することができる。
【００１５】
さらなる実施形態では、本発明は、上で定義したような本発明のポリペプチド又はキメラポリペプチドに対して結合特異性を有する抗体を産生させることのできるポリペプチドにも関する。
一実施形態では、表Ａ（BVH-3）又は表Ｄ（BVH-11）のポリペプチドは、少なくとも１つのエピトープ保持部を含む。
さらなる実施形態では、本発明のポリペプチドの断片は、表Ｃ及びＦで特定される１つ以上のエピトープ保持部を含む。断片は、表Ｃ及びＦの少なくとも15相接アミノ酸のポリペプチドを含む。断片は、表Ｃ及びＦの少なくとも20相接アミノ酸のポリペプチドを含む。
さらなる実施形態では、表Ａ（BVH-3）のポリペプチドのエピトープ保持部は、表Ｃに列挙されている少なくとも１つのポリペプチドを含む。
さらなる実施形態では、表Ｂ（BVH-11）のポリペプチドのエピトープ保持部は、表Ｆに列挙されている少なくとも１つのポリペプチドを含む。
【００１６】
“結合特異性を有する”抗体とは、選択されたポリペプチドを認識して結合するが、実質的に生体試料のような試料中の他の分子は認識かつ結合しない抗体である。特異的結合は、ELISAアッセイにより、その選択されたポリペプチドを抗原として使用して測定することができる。
特に定義しない場合、本明細書で用いるすべての技術的及び科学的用語は、本発明が属する技術の当業者が一般的に理解するのと同じ意味を有する。本明細書で言及するすべての公開公報、特許出願、特許、及びその他の参照文献は、参照によってその全体が本明細書に取り込まれる。矛盾する場合は、定義を含め、本明細書が支配する。さらに、材料、方法及び実施例は説明のためだけであり、限定する意図ではない。
【００１７】
本発明により、生物学的研究における“保護”は、生存曲線、率又は期間における有意な増加によって定義される。対数ランク試験を用いて生存曲線を比較する統計解析、及びフィッシャー正確試験を用いて生存率と死亡までの日数を比較する統計解析は、それぞれＰ値を計算し、かつ２群間の相異が統計的に意味があるかどうかを決定するのに有用である。Ｐ値0.05は、意味がないとみなされる。
【００１８】
本明細書で使用する場合、本発明のポリペプチドの“断片”、“誘導体”又は“類似体”は、１つ以上のアミノ酸残基が、保存又は非保存アミノ酸残基（好ましくは保存）で置換されているポリペプチドを包含し、天然又は非天然でよい。一実施形態では、本発明のポリペプチドの誘導体及び類似体は、図面に示される配列又はその断片と約70％の同一性を有する。すなわち、残基の70％が同一である。さらなる実施形態では、ポリペプチドは75％より高い相同性を有する。さらなる実施形態では、ポリペプチドは80％より高い相同性を有する。さらなる実施形態では、ポリペプチドは85％より高い相同性を有する。さらなる実施形態では、ポリペプチドは90％より高い相同性を有する。さらなる実施形態では、ポリペプチドは95％より高い相同性を有する。さらなる実施形態では、ポリペプチドは99％より高い相同性を有する。さらなる実施形態では、本発明のポリペプチドの誘導体及び類似体は、約20個未満、さらに好ましくは10個未満のアミノ酸残基置換、修飾又は欠失を有する。
【００１９】
好ましい置換は、保存型、すなわち置換される残基が疎水性、大きさ、電荷又は官能基のような物理的又は化学的性質を共有するとして当業者に公知の置換である。
当業者には、本発明のタンパク質又はポリペプチドの類似体又は誘導体が、本発明の文脈における用途、すなわち抗原性／免疫原性物質としての用途を見出すことが分かるだろう。従って、例えば１つ以上の付加、欠失、置換等を含むタンパク質又はポリペプチドは本発明に包含される。さらに、あるアミノ酸を同様の“タイプ”の別のアミノ酸で置換することができる。例えば、ある疎水性アミノ酸を別の疎水性アミノ酸と置換することである。
【００２０】
CLUSTALプログラムのようなプログラムを用いてアミノ酸配列を比較することができる。このプログラムは、アミノ酸配列を比較し、どちらかの配列に適するようにスペースを挿入することで最適なアラインメントを見つける。最適なアラインメントについてのアミノ酸の同一性又は類似性（同一性プラスアミノ酸タイプの保存）を計算することができる。BLASTxのようなプログラムは、同様な配列の最長の伸びを調整し、適合値を割り当てる。従って、それぞれ異なったスコアを有し、いくつかの領域の類似が見られる対照を得ることができる。本発明では、両タイプの同一性解析が熟考される。
これとは別のアプローチでは、類似体又は誘導体は、例えば所望のタンパク質又はポリペプチドを効率的に標識化することによって、精製しやすい部分を取り込んだ融合タンパク質である。その“標識”を除去する必要があるか、又は融合タンパク質自体が有用な十分の抗原性を保持する場合もある。
【００２１】
本発明のさらなる局面では、本発明のタンパク質若しくはポリペプチド、又はその類似体若しくは誘導体の抗原性／免疫原性断片が提供される。
本発明の断片は、１つ以上のこのようなエピトープ領域を含むか、又はこのよな領域に十分に類似し、その抗原性／免疫原性特性を保持すべきである。従って、本発明の断片については、それら断片が本明細書で述べるようなタンパク質若しくはポリペプチドの特定部分、類似体又は誘導体に100％同一でありうるので、同一性の程度はおそらく無関係である。この場合もやはり、重要な問題は、断片が抗原性／免疫原性特性を保持することである。
従って、類似体、誘導体及び断片について重要なことは、それらが誘導されるタンパク質又はポリペプチドと少なくとも同程度の抗原性／免疫原性を有することである。
【００２２】
本発明により、本発明のポリペプチドはポリペプチドとキメラポリペプチドの両方を包含する。
該ポリペプチドの生物学的又は薬理学的特性を変える他の成分、すなわち半減期を増やすためのポリエチレングリコール（PEG）；精製を容易にするためのリーダー又は分泌性アミノ酸配列；プレプロ−及びプロ−配列；及び（ポリ）サッカリドに融合しているポリペプチドも含まれる。
さらに、アミノ酸領域が多型性であることが分かっている場合、１つ以上の特定のアミノ酸を変えて、異なるストレプトコッカス菌株の異なるエピトープをより効率的に模倣することが望ましい。
【００２３】
さらに、本発明のポリペプチドは、末端-NH2アシル化（例えばアセチル化、又チオグリコール酸アミド化、例えばアンモニア若しくはメチルアミンによる末端カルボキシアミド化により）によって修飾して安定性を与え、すなわち支持体又は他の分子に連結又は結合するために疎水性を高めることができる。
該ポリペプチド断片、類似体及び誘導体のヘテロ及びホモポリペプチド多量体も熟考される。これらポリマー形態としては、例えば、アビジン／ビオチン、グルテルアルデヒド又はジメチルスーパーイミダートのような架橋剤で架橋されている１種以上のポリペプチドが挙げられる。このようなポリマー形態としては、組換えDNA技術で生成された多シストロンmRNAから生じる２種以上のタンデム又は反転相接配列を含有するポリペプチドが挙げられる。
【００２４】
好ましくは、本発明のポリペプチドの断片、類似体又は誘導体は、少なくとも１つの抗原性領域、すなわち少なくとも１つのエピトープを含む。
抗原性ポリマー（すなわち合成多量体）の形成を達成するため、ビスハロアセチル基、ニトロアリールハライド等を有するポリペプチドを利用でき、該試薬はチオ基に特異的である。従って、異なるペプチドの２個のメルカプト基間の連結は単結合であるか、又は少なくとも２個、典型的には少なくとも４個で、16個以下であるが、通常約14個以下の炭素原子の連結基で構成される。
【００２５】
特定の実施形態では、本発明のポリペプチド断片、類似体又は誘導体は、メチオニン（Met）開始残基を含まない。好ましくは、ポリペプチドはリーダー又は分泌配列（シグナル配列）を取り込まない。本発明のポリペプチドのシグナル部は、確立された分子生物学的技法に従って決定できる。一般に、関心のあるポリペプチドは、ストレプトコッカス培養から単離し、引き続き配列決定により成熟タンパク質の最初の残基、ひいては成熟ポリペプチドの配列を決定することができる。
【００２６】
別の局面により、医薬的に許容性のキャリヤー、希釈剤又はアジュバントが混合されて、本発明の１種以上のストレプトコッカスポリペプチドを含むワクチン組成物が提供される。好適なアジュバントとしては、油、すなわちフロイント完全アジュバント又は不完全アジュバント；塩、すなわちAlK(SO4)2、AlNa(SO4)2、AlNH4(SO4)2、シリカ、カオリン、カーボンポリヌクレオチド、すなわちポリIC及びポリAUが挙げられる。好ましいアジュバントとしては、Quila及びAlhydrogel
が挙げられる。本発明のワクチンは、注射、急速注入、鼻咽頭吸収、皮膚吸収、若しくは頬側によって非経口的に、又は経口的に投与することができる。医薬的に許容性のキャリヤーとして、破傷風トキソイドも挙げられる。
用語ワクチンは、抗体をも含む意である。本発明により、ストレプトコッカス感染及び／又はストレプトコッカス感染を媒介とする病気及び症状の治療又は予防のための、本発明のポリペプチドに対して結合特異性を有する１種以上の抗体の使用も提供される。
【００２７】
本発明のワクチン組成物は、レプトコッカス感染及び／又はP.R. Murray (主筆),E.J. Baron, M.A. Pfaller, F.C. Tenover及びR.H. Yolkenに記載されているようなストストレプトコッカス感染によって媒介される病気及び症状の治療又は予防のために用いられる。参照によって本明細書に取り込まれる臨床生物学のマニュアル,ASM Press,Washington, D.C. 第６版, 1995, 1482p。一実施形態では、本発明のワクチン組成物は、髄膜炎、中耳炎、菌血症又は肺炎の治療又は予防のために用いられる。一実施形態では、本発明のワクチン組成物は、ストレプトコッカス感染及び／又はストレプトコッカス感染、特にS.ニュモニエ、Ａ群ストレプトコッカス（ピオゲン）、Ｂ群ストレプトコッカス（GBS又はアガラクティエ）、ディスガラクティエ、ウレビス、ノカルジア及びストレプトコッカス・アウレウスによって媒介される病気又は症状の治療又は予防のために使用される。さらなる実施形態では、ストレプトコッカス感染は、S.ニュモニエである。
【００２８】
特定の実施形態では、ワクチンは胎児、高齢者及び免疫無防備状態の個体のようなストレプトコッカス感染の恐れのある個体に投与される。
本出願で使用する場合、用語“個体”は哺乳類を包含する。さらなる実施形態では、哺乳類はヒトである。
ワクチン組成物は、好ましくは約0.001〜100μg/kg（抗原／体重）、さらに好ましくは0.01〜10μg/kg、最も好ましくは0.1〜１μg/kgの単位剤形で、約１〜６週間の免疫化間の間隔で１〜３回投与される。
ワクチン組成物は、好ましくは約0.1μg〜10mg、さらに好ましくは１μg〜１mg、最も好ましくは10〜100μgの単位剤形で、約１〜６週間の免疫化間の間隔で１〜３回投与される。
【００２９】
別の局面により、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択されるアミノ酸配列を特徴とするポリペプチド、又はその断片、類似体若しくは誘導体をコードするポリヌクレオチドが提供される。
別の局面により、表Ｂ、Ｅ若しくはＨから選択されるアミノ酸配列を特徴とするポリペプチド、又はその断片、類似体若しくは誘導体をコードするポリヌクレオチドが提供される。
一実施形態では、ポリヌクレオチドは、本発明のポリペプチドをコードする、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ又はＨに示されるものである。
一実施形態では、ポリヌクレオチドは、本発明のポリペプチドをコードする、表Ｂ、Ｅ又はＨに示されるものである。
【００３０】
図に示されるポリヌクレオチド配列は、縮重したコドンによって変化しうるが、依然として本発明のポリペプチドをコードしていることが明かである。従って、本発明は、さらに配列間に50％の同一性を有する上述のポリヌクレオチド配列（又はその相補的配列）にハイブリダイズするポリヌクレオチドを提供する。一実施形態では、配列間の少なくとも70％の同一性。実施形態では、配列間の少なくとも75％の同一性。一実施形態では、配列間の少なくとも80％の同一性。一実施形態では、配列間の少なくとも85％の同一性。一実施形態では、配列間の少なくとも90％の同一性。さらなる実施形態では、ポリヌクレオチドは、ストリンジェント条件、すなわち少なくとも95％の同一性を有する条件下でハイブリダイズ可能である。さらなる実施形態では、97％より高い同一性である。
当業者は、ハイブリダイゼーションに適したストリンジェント条件を容易に決めることができる（例えば、Sambrookら,（1989）分子クローニング: 実験室マニュアル,第２版, Cold Spring Harbor, N.Y.;分子生物学の最新プロトコル ,(1999) Ausubel F.M.ら編集, John Wiley & Sons, Inc., N.Y.参照)。
【００３１】
さらなる実施形態では、本発明は、ストリンジェント条件下で
（ａ）ポリペプチドをコードするDNA配列又は
（ｂ）ポリペプチドをコードするDNA配列の補体
のどちらかにハイブリダイズするポリヌクレオチドであって、
前記ポリペプチドが表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択される配列又はその断片又は類似体を含む、ポリヌクレオチドを提供する。
さらなる実施形態では、本発明は、ストリンジェント条件下で
（ａ）ポリペプチドをコードするDNA配列又は
（ｂ）ポリペプチドをコードするDNA配列の補体
のどちらかにハイブリダイズするポリヌクレオチドであって、
前記ポリペプチドが表Ｂ、Ｅ若しくはＨから選択される配列又はその断片又は類似体を含む、ポリヌクレオチドを提供する。
【００３２】
さらなる実施形態では、本発明は、ストリンジェント条件下で
（ａ）ポリペプチドをコードするDNA配列又は
（ｂ）ポリペプチドをコードするDNA配列の補体
のどちらかにハイブリダイズするポリヌクレオチドであって、
前記ポリペプチドが表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨから選択される配列又はその断片又は類似体を含むポリペプチド由来の少なくとも10個の相接アミノ酸残基を含む、ポリヌクレオチドを提供する。
さらなる実施形態では、本発明は、ストリンジェント条件下で
（ａ）ポリペプチドをコードするDNA配列又は
（ｂ）ポリペプチドをコードするDNA配列の補体
のどちらかにハイブリダイズするポリヌクレオチドであって、
前記ポリペプチドが表Ｂ、Ｅ若しくはＨから選択される配列又はその断片又は類似体を含むポリペプチド由来の少なくとも10個の相接アミノ酸残基を含む、ポリヌクレオチドを提供する。
【００３３】
さらなる実施形態では、ポリヌクレオチドは、表Ａ、Ｂ、Ｄ、Ｅ、Ｇ若しくはＨに示される本発明のポリペプチドをコードするポリヌクレオチドである。
当業者には容易に分かるように、ポリヌクレオチドには、DNAとRNAの両者を包含する。
本発明は、本出願で述べるポリヌクレオチドに相補的なポリヌクレオチドをも包含する。
【００３４】
さらなる局面では、本発明のポリペプチド又はその断片、類似体若しくは誘導体をコードポリヌクレオチドは、DNA免疫化法に使用できる。すなわち、それらを注射すると複製かつ発現可能なベクター中に取り込み、それによって抗原性ポリペプチドをインビボ産生することができる。例えば、真核生物細胞内で機能的であるCMVプロモーターの制御下プラスミドベクター中にポリヌクレオチドを取り込むことができる。好ましくは、該ベクターは筋肉内注射される。
【００３５】
別の局面により、本発明のポリペプチドの製造方法であって、前記ポリペプチドをコードするポリヌクレオチドを宿主内で発現させ、その過剰発現されたポリペプチド産物を回収することによる組換え法による方法が提供される。これとは別に、ポリペプチドは、確立された化学合成法、すなわち結合されて全ポリペプチドを生成する（ブロック連結反応）オリゴヌクレオチドの液相又は固相合成によって製造することができる。
【００３６】
ポリヌクレオチド及びポリペプチドの獲得及び評価の一般的な方法は、以下の参照文献に記載されている：Sambrookら,分子クローニング:実験室マニュアル, 第２版, Cold Spring Harbor, N.Y., 1989;分子生物学の最新プロトコル, Ausubel F.M.ら編集, John Wiley & Sons, Inc. New York; PCRクローニングプロトコル,分子クローニングから遺伝子工学, White B.A.編集, Humana Press, Totowa, New Jersey, 1997, 490ページ;タンパク質精製,原理と実施, Scopes R.K., Springer-Verlag, New York,第３版, 1993, 380ページ;免疫学の最新プロトコル, Coligan J.E.ら編集, John Wiley & Sons Inc., New York。これら文献は、参照によって本明細書に取り込まれる。
【００３７】
組換え製造では、ポリペプチドをコードするベクターで宿主を形質移入してから、プロモーターを活性化し、形質転換体を選択し、又は遺伝子を増幅するのに適するように修飾した栄養培地で培養する。好適なベクターは、選択した宿主内で生存可能かつ複製可能なベクターであり、染色体性、非染色体性及び合成DNA配列、例えば細菌性プラスミド、ファージDNA、バキュロウィルス、酵母プラスミド、プラスミドとファージDNAを組み合わせて誘導されるベクターが挙げられる。制限酵素を用いて、プロモーター、リボゾーム結合部位（コンセンサス領域又はシャイン-ダルガルノ配列）、及び任意にオペレーター（制御要素）を含む発現制御領域に操作的にポリペプチド配列が連結されるように、ポリペプチド配列をベクターの適切な部位に取り込むことができる。所定の宿主及びベクターに適する発現制御領域の個々の成分は、確立された分子生物学の原理に従って選択することができる（参照によって本明細書に取り込まれているSambrook,ら,分子クローニング：実験室マニュアル,第２版,Cold Spring Harabor,N.Y.,1989；分子生物学の最新プロトコル,Ausubel F.M.ら編集, John Wiley & Sons, Inc.,N.Y.）。好適なプロモーターとしては、限定するものではないがLTR又はSV40プロモーター、大腸菌lac、tac又はtrpプロモーター及びファージlambda PLプロモーターが挙げられる。ベクターは、好ましくは複製及び選択マーカの起源、すなわちアンピシリン抵抗性遺伝子を取り込む。好適な細菌性ベクターとしては、pET、pQE70、pQE60、pQE-9、pbs、pD10 phagescript、psiX174、pbluescript SK、pbsks、pNH8A、pNH16a、pNH18A、pNH46A、ptrc99a、pKK223-3、pKK233-3、pDR540、pRIT5及び真核生物ベクターpBlueBacIII、pWLNEO、pSV2CAT、pOG44、pXT1、pSG、pSVK3、pBPV、pMSG及びpSVLが挙げられる。宿主細胞は、細菌、すなわち大腸菌、バシラス・サチリス、ストレプトマイセス；真菌、すなわちアスペルギルス・ニガー、アスペルギルス・ニデュランス；酵母菌、すなわちサッカロミセス又は真核生物、すなわちCHO、COSが挙げられる。
【００３８】
培養でポリペプチドが発現されると、典型的には細胞を遠心分離で収穫し、物理的又は化学的手段によって破壊し（発現されたポリペプチドが培地中に分泌されていない場合）生成した粗抽出物を保持して関心のあるポリペプチドを単離する。培地又はライセートからのポリペプチドの精製は、該ポリペプチドの特性によって決まる確立された方法、すなわち硫酸アンモニウム若しくはエタノール沈殿、酸抽出、アニオン若しくはカチオン交換クロマトグラフィー、ホスホセルロースクロマトグラフィー、疎水性相互作用クロマトグラフィー、ヒドロキシルアパタイトクロマトグラフィー及びレクチンクロマトグラフィーによって達成することができる。最終精製はHPLCを用いて達成できる。
【００３９】
ポリペプチドは、リーダー又は分泌配列により、又はこれら配列なしで発現されうる。前者の場合、リーダーを翻訳後プロセシングで除去するか（参照によって本明細書に取り込まれる米国特許第4,431,739号；第4,425,437号；及び第4,338,397号参照）又は化学的に除去した後、発現ポリペプチドを精製する。
さらなる局面により、本発明のストレプトコッカスポリペプチドは、ストレプトコッカス感染、特にS.ニュモニエ感染のための診断試験に使用できる。いくつかの診断方法が可能であり、例えば生体試料中のストレプトコッカス生物体を検出する方法であり、以下の手順に従う：
ａ）患者から生体試料を得；
ｂ）本発明のストレプトコッカスポリペプチドと反応性の抗体又はその断片を、前記生体試料と共にインキュベートして混合物を生成し；かつ
ｃ）ストレプトコッカスの存在を示、前記混合物内で特異的に結合される抗体又は断片を検出する。
【００４０】
これとは別に、前記抗体を含有するか、又は含有すると疑われる生体試料内におけるストレプトコッカス抗原に特異的な抗体の検出方法は、以下のように行われる：
ａ）患者から生体試料を得；
ｂ）１種以上の本発明のストレプトコッカスポリペプチド又はその断片を、前記生体試料と共にインキュベートして混合物を生成し；かつ
ｃ）ストレプトコッカスに特異的な抗体の存在を示す、前記混合物内で特異的に結合される抗体又は断片を検出する。
当業者は、この診断試験がエンザイム-リンクドイムノソルベントアッセイ（ELISA）、ラジオイムノアッセイ又はラテックス凝集アッセイのような免疫学的試験を含むいくつかの形態を取り、基本的に該ポリペプチドに特異的な抗体が生物体内に存在するかどうかを決定することを認識している。
【００４１】
本発明のポリペプチドをコードするDNA配列を用い、ストレプトコッカスを含有すると疑われる生体試料中の該細菌の存在を検出するためのDNAプローブを設計することができる。この発明の検出方法は、以下の工程を含む：
ａ）患者から生体試料を得る工程；
ｂ）本発明のポリペプチド又はその断片をコードするDNA配列を有する１種以上のDNAプローブを、前記生体試料と共にインキュベートして混合物を生成する工程；及び
ｃ）ストレプトコッカス細菌の存在を示す、前記混合物内で特異的に結合されるDNAプローブを検出する工程。
【００４２】
この発明のDNAプローブは、例えば、ストレプトコッカス感染の診断法としてポリメラーゼ連鎖反応を用いる循環ストレプトコッカス検出、すなわち試料中のS.ニュモニエ核酸の検出に使用できる。
プローブは、従来法によって合成でき、固相上に固定化するか、又は検出可能な標識で標識化できる。この出願で好ましいDNAプローブは、本発明のストレプトコッカス・ニュモニエポリペプチドの少なくとも約６個の相接ヌクレオチドに相補的な配列を有するオリゴマーである。
【００４３】
患者のストレプトコッカス検出の別の診断方法は以下の工程を含む：
ａ）本発明のポリペプチド又はその断片と反応性の抗体を、検出可能な標識で標識化する工程；
ｂ）この標識化抗体又は標識化断片を患者に投与する工程；及び
ｃ）ストレプトコッカスの存在を示す、前記患者内で特異的に結合される標識化抗体又は標識化断片を検出する工程。
【００４４】
本発明のさらなる局面は、本発明のストレプトコッカスポリペプチドの、ストレプトコッカス感染の診断及び特に治療用の特異的抗体の製造のための免疫原としての使用である。好適な抗体は、適切なスクリーニング方法、例えば特定抗体の試験モデル内におけるストレプトコッカス感染に対して受動的に保護する能力を測定することによって決定できる。動物モデルの一例は本明細書の実施例に記載されているマウスである。抗体は、全体的な抗体又はその抗原結合性断片でよく、いずれの免疫グロブリン分類に属してもよい。抗体又は断片は動物起源、具体的には哺乳類起源、さらに詳細にはマウス、ラット又はヒト起源でよい。それは、天然抗体若しくは抗体断片でよく、又は所望により、組換え抗体若しくは抗体断片でよい。用語組換え抗体又は抗体断片は、分子生物学的方法を用いて生成された抗体又は抗体断片を意味する。抗体又は抗体断片はポリクロナールでよく、好ましくはモノクロナールである。それは、ストレプトコッカス・ニュモニエポリペプチドに関連する多くのエピトープに特異的であってよいが、好ましくは１つのエピトープに特異的である。
【００４５】
本発明のさらなる局面は、本発明のストレプトコッカスポリペプチドに向けられる抗体の、受動免疫化のための使用である。本出願で述べる抗体を使用することができる。好適な抗体は、適切なスクリーニング方法、例えば特定抗体の試験モデル内におけるストレプトコッカス感染に対して受動的に保護する能力を測定することによって決定できる。動物モデルの一例は本明細書の実施例に記載されているマウスである。抗体は、全体的な抗体又はその抗原結合性断片でよく、いずれの免疫グロブリン分類に属してもよい。抗体又は断片は動物起源、具体的には哺乳類起源、さらに詳細にはマウス、ラット又はヒト起源でよい。それは、天然抗体若しくは抗体断片でよく、又は所望により、組換え抗体若しくは抗体断片でよい。用語組換え抗体又は抗体断片は、分子生物学的方法を用いて生成された抗体又は抗体断片を意味する。抗体又は抗体断片はポリクロナールでよく、好ましくはモノクロナールである。それは、ストレプトコッカス・ニュモニエポリペプチドに関連する多くのエピトープに特異的であってよいが、好ましくは１つのエピトープに特異的である。
以下は、本出願で開示する配列をまとめた参照表である。
【００４６】
表Ａ、Ｂ及びＣ BVH-3-の変異体及びエピトープ
表Ａ
0005051959.tif 000002
【００４７】
表Ｂ
0005051959.tif 000003
**サイレント突然変異、すなわちポリペプチドはNew1又はNew56と同一である。
【００４８】
表Ｃ BVH-3のエピトープ
0005051959.tif 000004
【００４９】
表Ｄ、Ｅ及びＦ BVH-3-の変異体及びエピトープ
表Ｄ
0005051959.tif 000005
【００５０】
表Ｅ
0005051959.tif 000006
【００５１】
表Ｆ BVH-3のエピトープ
0005051959.tif 000007
【００５２】
表Ｇ及びＨ キメラ−
表Ｇ
0005051959.tif 000008
* 任意のアミノ酸
【００５３】
表Ｈ
0005051959.tif 000009
【００５４】
実施例１
この実施例は、ここで使用する細菌株、プラスミド、PCRプライマー、組換えタンパク質及びハイブリドーマ抗体について述べる。
S.ニュモニエ SP64（血清型６）及びSP63（血清型９）の臨床用単離物は、Laboratoire de la Sante Publique du Quebec, Sainte-Anne-de-Bellevueによって提供され；Rx1株、２型株D39及び３型株WU2の非被包誘導体は、アラバマ大学, バーミンガムからDavid.E.Bribesによって提供され、３型臨床用単離物P4241はCentre de Recherche en Infectiologie du Centre Hospitalier de lUniversite Laval, Sainte-Foyから提供された。大腸菌株DH5α (Gibco BRL, Gaithesburg, MD); AD494 (λDE3) (Novagen, Madison, WI)及びBL21 (λDE3) (Novagen)並びにプラスミド超リンカーpSL301ベクター(Invitrogen, San Diego, CA);pCMV-GHベクター(テキサス州ダラスのテキサス大学生化学部のStephen A. Johnston博士から贈呈);pET32及びpET21 (Novagen)及びpURV22.HIS発現ベクター(図３０)をこの研究で使用した。pURV22.HISベクターは、機能性ＰRプロモーターが欠失されているバクテリオファージλ cI857温度感受性リプレッサー遺伝子のカセットを含有する。プロモーターλPLの制御下、30〜37℃から37〜42℃の範囲で昇温することでcI857リプレッサーを不活性化すると該遺伝子が誘発される。組換えプラスミドの生成に用いるPCRプライマーは、５'末端に制限エンドヌクレアーゼ部位を有し、それによって増殖幅産物の消化プラスミドベクターへの方向性クローニングが可能になる。下表１に、使用するPCRオリゴヌクレオチドプライマーがリストされている。BVH-3、BVH-11及びBVH-11-2遺伝子産物をコード化する遺伝子配列の配置は、図２５、図２６及び図２７にそれぞれまとめられている。
【００５５】
表１ PCRオリゴヌクレオチドプライマーのリスト
0005051959.tif 000010
0005051959.tif 000011
0005051959.tif 000012
0005051959.tif 000013
0005051959.tif 000014
【００５６】
標準的な方法に従って分子生物学技法を行った。例えば、参照によって本明細書に取り込まれる、Sambrook, J., Fritsch, E.F., Maniatis, T.,“分子クローニング 実験室マニュアル”Vol.1-2-3(第２版) Cold Spring Harbour Laboratory Press, 1989, New Yorkを参照せよ。PCR増幅産物が制限エンドヌクレアーゼで消化し、同様に消化され、又は適合性付着末端を生成する酵素で消化された線状プラスミドpSL301、pCMV-GH、pET若しくはpURV22.HIS発現ベクターに結合した。組換えpSL301及び組換えpCMV-GHプラスミドをpET発現ベクター中のインフレームクローニング用の制限酵素で消化した。pETベクターを使用する場合、クローンを全長又は不完全BVH-3、BVH-11又はBVH-11-2分子の発現のために大腸菌BL21（λDE3）又はAD494（λDE3）中に導入する前に、まず大腸菌DH5α内に固定した。結果として生じた各プラスミド構成物をヌクレオチド配列解析で確認した。組換えタンパク質は、チオレドキシン及びHis-tag（pET32発現系）とのＮ末端融合；His-tag（pET21発現系）とのＣ末端融合；又はHis-tag（pURV22.HIS発現系）とのＮ末端融合として発現した。発現組換えタンパク質は、超音波処理IPTG-（pET系）又はHis-Bind金属キレーション樹脂(QIAgen, Chatsworth, CA)を用いる熱-（pURV22.HIS）誘発大腸菌の遠心分離後に得られる上清フラクションから精製した。S.ニュモニエSP64から産生された遺伝産物は、表２にリストされる。BVH-3-Sp63タンパク質(配列番号:10のアミノ酸残基21〜840)をコードする遺伝子断片は、PCR-プライマーセットOCRR479-OCRR480及びクローニングベクターpSL301を用いてS.ニュモニエSP63から生成した。BVH-3-Sp63分子発現用pET32ベクター内におけるインフレームクローニングのため組換えpSL301-BVH-3Sp63が消化された。
【００５７】
表２. S.ニュモニエSP64から産生された不完全なBVH-3、BVH-11、BVH-11-2及びキメラ遺伝子産物のリスト
0005051959.tif 000015
0005051959.tif 000016
w/o ss：シグナル配列なし。BVH-3、BVH-11及びBVH-11-2タンパク質配列の解析は、推定疎水性リーダー配列の存在を示唆した。
* キメラについてコードされるアミノ酸は遺伝子産物として発現され、加えられた付加非必須アミノ酸残基Ｍはメチオニン、Ｋはリジン、Ｌはロイシン、Ｇはグリシン及びＰはプロリンである。
【００５８】
ハイブリドーマを分泌するモノクロナール抗体(Mab)は、Fazekas De St-Groth and Scheidegger (J Immunol Methods 35: 1-21, 1980)を改変した(J. Hamel et al. J Med Microbiol 23: 163-170, 1987)方法により、免疫化したマウス由来の脾臓細胞と、非分泌HGPRT-欠乏マウス骨髄腫SP2/0細胞の融合によって得た。メスのBALB/cマウス(Charles River, St-Constant, Quebec, Canada)を、S.ニュモニエ株SP64由来のBVH-3M(チオレドキシン-His・Tag-BVH-3M融合タンパク質/pET32系)、BVH-11M(チオレドキシン-His・Tag-BVH-11M融合タンパク質/pET32系)、BVH-11-2M(チオレドキシン-His・Tag-BVH-11-2M融合タンパク質/pET32系)、BVH-11B(チオレドキシン-His・Tag-BVH-11B融合タンパク質/pET32系)、BVH-3M(His・Tag-BVH-3融合タンパク質/pURV22.HIS系)又はNEW1(NEW1-His・Tag融合タンパク質/pET21系)遺伝子産物で免疫化して、それぞれMabシリーズH3-、H11-、H112-、H11B-、H3V-、及びHN1-を得た。ハイブリドーマの培養上清は、まず、Hamelら(前出)に記載されている手順に従い、精製組換えBVH-3、BVH-11及び／又はBVH-11-2タンパク質又は熱殺S.ニュモニエ細胞の懸濁液の製剤を塗布したプレートを用いて酵素結合イムノアッセイ(ELISA)によってスクリーニングした。さらに特徴づけするために選択したMab-分泌ハイブリドーマは、以下の実施例２から表３及び表４にリストされている。Mab免疫グロブリンのクラス及びサブクラスは、商業的に入手可能な試薬(Southern Biotechnology Associates, Birmingham, AL)を用いてELISAによって決定した。
【００５９】
さらに、キメラポリペプチドをコードするキメラ遺伝子のクローニングと発現及びこれらキメラポリペプチドによるワクチン接種後に観察された保護について述べる。
操作してキメラ遺伝子内に包含されるべき遺伝子断片を増幅したオリゴヌクレオチド対を用い、遺伝子の３'末端に対応するBVH-3及びBVH-11遺伝子断片をPCRで増幅した。用いたプライマーは５'末端に制限エンドヌクレアーゼ部位を有し、その結果として増幅産物の消化プラスミドベクターへの方向性インフレームクローニングが可能になった（表１及び表２）。PCR-増幅産物を制限エンドヌクレアーゼで消化し、線状プラスミドpET21又はpSL301ベクターに結合した。結果のプラスミド構成物をヌクレオチド配列解析で確認した。PCR産物を含有する組換えpET21プラスミドを、第２DNA断片のインフレームクローニング及びキメラ肺炎球菌タンパク質分子をコードするキメラ遺伝子産生のための制限酵素による消化で線状にした。PCR産物を含有する組換えpSL301プラスミドを、DNA挿入断片を得るための制限酵素で消化した。生成した挿入DNA断片を精製し、所定のキメラ遺伝子に対応する挿入断片をキメラ遺伝子の産生用pET21ベクターに結合した。表２にリストされている組換えキメラポリペプチドは、His-tagとのＣ末端融合としてだった。His-Bind金属キレーション樹脂(QIAgen, Chatsworth, CA)を用いて超音波処理IPTG-誘発大腸菌培養の遠心分離から得た上清フラクションから発現組換えタンパク質を精製した。
【００６０】
８匹のメスのBALB/cマウス(Charles River)群を、15〜20μgのQuilAアジュバントの存在下同定されたいずれかのアフィニティ精製His・Tag-融合タンパク質25μgで３週間の間隔をおいて２回皮下的に免疫化した。最後の免疫化後10〜14日に、マウスに、S.ニュモニエ３型株WU2の10E5-10E6 CFUを静脈内投与した。このポリペプチド及び断片は保護免疫応答を誘発可能である。
【００６１】
表 2A
0005051959.tif 000017
【００６２】
実施例２
この実施例は、Mabs及び実施例１で述べた組換え不完全タンパク質を用いた、標的エピトープを保有するペプチドドメインの同定について述べる。
Mabsによって認識されるエピトープを特徴づけるため、不完全なBVH-3、BVH-11又はBVH-11-2遺伝子産物に対してELISAで試験した。不完全遺伝子産物は、S.ニュモニエSP63株から産生されたBVH-3-Sp63を除き、S.ニュモニエSP64株から産生された。ポジティブ対照として、各抗体の反応性を全長BVH-3、BVH-11又はBVH-11-2組換えタンパク質で調べた。数ケースでは、SDS-PAGE及びニトロセルロース紙上の移動による遺伝子産物の分離後ウェスタンイムノブロット法によってMab反応性を評価した。観察された反応性は、以下の表３及び表４に示される。
【００６３】
表３．BVH-3-反応性Mabsと11種のBVH-3遺伝子産物及びBVH-11M分子グループとのELISA反応性
0005051959.tif 000018
【００６４】
（表３の続き）
0005051959.tif 000019
NT：試験せず
+/-：非常に低い反応性であるが、バックグラウンドより高く、おそらく非特異的Mab結合性
【００６５】
0005051959.tif 000020
【００６６】
0005051959.tif 000021
【００６７】
0005051959.tif 000022
【００６８】
0005051959.tif 000023
【００６９】
エピトープの推定位置は、図２８及び図２９に要約されている。表３のデータから分かるように、BVH-3-反応性Mabsは、BVH-3A及びBVH-3B分子の両方と反応するH11-TG11及びH3V-15A10を除き、２グループ、すなわちBVH-3A-及びBVH-3B-反応性Mabsに分割できる。BVH-3A-反応性Mabsは、BVH-3C組換えタンパク質との反応性又は反応性欠如によって、２サブグループの抗体にサブ分割できる。BVH-3Cタンパク質と反応性のMabは、BVH-3及びBVH-11タンパク質の両方で共有されるエピトープを認識した。表４で見られるように、これらBVH-3-及びBVH-11-交差反応性Mabsは、BVH-11A及びBVH-11-2M組換えタンパク質とも反応性だった。BVH-3B-反応性Mabsは、それぞれNEW1、NEW2及びNEW3組換えタンパク質との反応性に応じて３サブグループにサブ分割できる。いくつかのMabsは、NEW1タンパク質とだけ反応性であるが、他のMabsはNEW1とNEW2又はNEW1とNEW3組換えタンパク質と反応性だった。
【００７０】
H11-7G11及びH3V-15A10は、BVH-3上の１箇所より多くの位置のエピトープと反応する。H11-7G11のBVH-3AD、BVH-3B、BVH-3C、BVH-11A及びBVH-11-2M分子との反応性は、H11-7G11エピトープがHXXHXH配列を含むことを示唆している。この配列は、BVH-3及びBVH-11/BVH-11-2タンパク質配列中、それぞれ６及び５回反復される。Mab H11-7G11のNEW10分子との反応性の欠如は、該エピトープがHGDHXH配列を含むことを示唆している。BVH-3上のH3V-15A10エピトープの複数位置マッピングは、Mabと、重ならない２個のBVH-3断片との反応性によって示唆される。
【００７１】
興味深いことに、Mabs H3-7G2、H3V-9C6及びH3V-16A7はBVH-3 Sp63と反応性でないので、S.ニュモニエSP64のBVH-3分子上のアミノ酸244と420との間に含まれる177-アミノ酸断片上に、それらの対応エピトープを配置できる（図３１）。
表４のデータから分かるように、BVH-11-及び／又はBVH-11-2と反応性であり、かつBVH-3分子を認識しないMabsは、そのBVH-11A及びNEW10組換えタンパク質との反応性に応じて３グループに分割できる。いくつかのMabsはBVH-11A又はNEW10組換えタンパク質のどちらかと排他的に反応するが、他のMabsはBVH-11AとNEW10組換えタンパク質の両方と反応性だった。
【００７２】
実施例３
この実施例は、エピトープのマッピング用のBVH-3及びBVH-11-2遺伝子ライブラリーの構成について述べる。
BVH-3及びBVH-11-2遺伝子ライブラリーは、それぞれヌクレオチド1837〜4909(配列番号:２)にわたるBVH-3遺伝子配列又はヌクレオチド172〜2630にわたるBVH-11-2遺伝子配列(配列番号:５)を含有する組換えpCMV-GH及びPSL301プラスミドDNAと、Novatope(登録商標)ライブラリー構成及びスクリーニングシステム(Novagen)を用いて構築した。BVH-3又はBVH-11-2遺伝子断片を含有する組換えプラスミドは、QIAgenキット(Chatsworth,CA)を用いて精製し、それぞれ制限酵素BglII及びXbaIで消化した。生成したBglII-XbaI DNA断片はQIAgen製のQIAquickゲル抽出キットを用いて精製し、ランダム切断DNAの生成のためDnase Iで消化した。50〜200塩基対のDNA断片を精製し、T4 DNA ポリメラーゼで処理して標的DNA末端に平滑化し、単一の3'dA残基を加え、製造業者によって示される手順に従って(Novatope(登録商標)システム, Novagen)pSCREEN-T-ベクター(Novagen)に結合した。それぞれBVH-3又はBVH-11-2遺伝子由来の小さいエピトープを発現する大腸菌クローンの遺伝子ライブラリーは、免疫プローブとしてMabsを用いる標準的なコロニーリフト法によってスクリーニングした。コロニースクリーニングは、非常に高いバックグラウンドをコロニーリフトに生じさせるMabsでは成功しなかった。さらに、エピトープ発現コロニーの検出に失敗したMabsもあった。反応性の欠如は、おそらく産生される組換えタンパク質の量が少ないこと、又は異なるタンパク質ドメインから成るコンホメーション依存性エピトープの認識によって説明できる。ポジティブクローンからのDNA挿入断片の配列決定によって標的エピトープをコードするセグメントの位置を決定した。データは表５に示される。組換えpSCREEN-Tベクター中へのDNA挿入によってコードされるポリペプチドを精製し、後述する実施例６で免疫原として使用することができる。
【００７３】
MabsによるBVH-3及びBVH-11-2遺伝子ライブラリーのスクリーニングから得られたペプチド配列は、不完全遺伝子産物に対するMabのELISA反応性と一致する。予想通り、H11-7G11から得られたアミノ酸配列は配列HGDHXHを含んでいた。この知見は、Mabsに認識されるエピトープの位置のさらなる証拠を与える。興味深いことに、Mabs H112-10G9、H112-10A2及びH11B-11B8は、同一のペプチド配列(BVH-11-2タンパク質配列上のアミノ酸残基594〜679)に対して反応性であるが、アミノ酸残基658〜698にわたる配列に対応するクローンは、Mab H11B-11B8によってしか選択されず、従ってアミノ酸残基658〜679(配列番号:163)間のH11B-11B8 エピトープの位置を明らかにした。Mabs H112-10G9、H112-10A2、及びH11B-11B8は、アミノ酸残基594〜679(配列番号:22)に対応するペプチド配列上に近接して位置する３つの別個の非オーバーラッピングエピトープに対して配向される。
【００７４】
表５．MabsによりBVH-3及びBVH-11-2遺伝子ライブラリーから得られたペプチド配列
0005051959.tif 000024
【００７５】
（表５の続き）
0005051959.tif 000025
【００７６】
実施例４
この実施例は、BVH-3、BVH-11及び／又はBVH-11-2と反応性の抗体を生産するための組換えタンパク質による動物の免疫化について述べる。
NZWウサギ(Charles River Laboratories, St-Constant, Quebec, Canada)を80μgのQuilAアジュバント(Cedarlane Laboratoratories Ltd, Hornby, Canada)の存在下50μg又は100μgの精製BVH-3M、L-BVH-3AD、NEW1、NEW13、又はL-BVH-11組換えタンパク質で、複数部位に皮下的に免疫化した。これらウサギを、３週間の間隔をおいて２回、同一抗原で追加免疫化し、各免疫化前及び最後の免疫化後６〜28日に血液試料を収集した。血清試料を免疫前、後1st、後2nd、又は後3rd注射と名付けた。免疫化に対するウサギの免疫応答は、塗布抗原として組換えBVH-3M(BVH-3M-His・Tag 融合タンパク質/pET21系)若しくはBVH-11M(BVH-11M-His・Tag融合タンパク質/pET21系)タンパク質又は熱殺S.ニュモニエRx-1細胞の懸濁液を用いてELISAによって評価した。ELISA力価は、吸収Ａ410値がバックグラウンド値より0.1超える最高の血清希釈の逆数として定義した。下表６に示されるように、全動物で免疫化後BVH-3及び／又はBVH-11エピトープと反応性の抗体が誘発された。組換え又は肺炎球菌抗原と反応性の抗体は、免疫前血清には存在しなかった。組換え抗原の単一注射後、各ウサギの血清中で免疫化に対する免疫応答が検出できた。試験した各抗原による第２注射後の抗体応答は、抗体力価の大きな増加を特徴とした。興味深いことに、S.ニュモニエと反応性の抗体の良い力価、第３免疫化後に52,000の平均力価が得られ、従って天然の肺炎球菌エピトープが組換え大腸菌遺伝子産物上で発現されることを立証した。これらデータは、BVH-3、BVH-11及び／又はBVH-11-2遺伝子産物、及び肺炎球菌病の予防及び治療用ワクチンとしてそれぞれBVH-3、BVH-11及び／又はBVH-11-2遺伝子産物に対して産生される抗体の潜在的な用途を支持する。
【００７７】
表６．BVH-3及びBVH-11遺伝子産物による免疫化に対するウサギ抗体応答
0005051959.tif 000026
NT：試験せず
【００７８】
実施例５
この実施例は、BVH-3、BVH-11又はBVH-11-2遺伝子産物に対して産生される抗体の投与による致死実験的肺炎球菌感染に対する動物の保護について述べる。
Brodeurら(J Immunol Methods 71:265-272,1984)によって記載された方法に従い、Mab分泌ハイブリドーマ細胞で腹腔内に接種されたマウスの腹水から、高力価Mab製剤を得た。実施例４で述べたようにBVH-3Mで免疫化したウサギから血清試料を収集した。第３免疫化後に収集したウサギ血清及び腹水を、45〜50％飽和硫酸アンモニウムを用いる沈殿による抗体の精製に使用した。抗体製剤をリン酸緩衝食塩水(PBS)に溶解し、透析した。
【００７９】
CBA/N (xid)マウス(National Cancer Institute, Frederick, MA)に、 0.1mlの精製ウサギ抗体又は0.2mlの腹水を腹腔内注射後、約200CFUの３型S.ニュモニエ株WU2を静脈内誘発した。対照マウスは無菌PBS又は免疫前ウサギ血清又は無関係なN.髄膜炎組換えタンパク質抗原で免疫化したウサギ由来の血清から精製された抗体を受けた。１グループのマウスは、抗-BVH-3の投与前にS.ニュモニエで誘発した。S.ニュモニエ誘発種菌の試料をチョコレート寒天プレート上に塗布し、CFU数を決定し、かつ誘発用量を検証した。S.ニュモニエ感染に対する高い感受性のため、CBA/Nマウスを選択した。CBA/Nマウスに静脈内注射したWU2のLD50は、≦10CFUと推定される。死は、少なくとも７日間に24時間の間隔で記録された。
【００８０】
ウサギ抗-BVH-3抗体を注射したマウスから得た保護データは、下表７に示される。抗-BVH-3抗体を受けた10匹のマウスのうち９匹が誘発に生き残ったのに対し、対照抗体又はPBS緩衝液を注射した10匹のマウスはゼロだった。誘発後に投与した場合でさえ、BVH-3-M分子に対して産生される抗体が受動的に保護するという観察は、既に確立された感染からさえ死を阻止するという抗-BVH-3抗体の能力を実証した。
【００８１】
表７．WU27肺炎球菌で静脈内誘発されたCBA/NマウスにおけるBVH-3-M遺伝子に対するウサギ抗体の保護効果
0005051959.tif 000027
0.1mlのウサギ抗体の腹腔内投与前後に、1000CFUのWU2 S.ニュモニエでCBA/Nマウスを感染させた。
【００８２】
他の実験では、免疫前及び免疫血清から調製された0.1mlのウサギ抗体を、280CFUのS.ニュモニエP4241 ３型株による鼻腔内誘発の４時間前にCBA/Nマウスに腹腔内投与した。下表８に示されるように、すべての免疫化マウスが誘発に生き残ったが、免疫前血清抗体又は緩衝液のみを受けた９匹のマウスはいずれも感染後６日生存しなかった。誘発後11日のS.ニュモニエ血液培養は、すべての残存マウスでネガティブだった。さらに、BVH-11/BVH-11-2に対して向けられるモノクロナール抗体H112-10G9又はH112-10G9とH11B-7E11の混合物を受けたマウスで100％保護が観察された。
【００８３】
表８．P4241肺炎球菌で鼻腔内誘発されたCBA/NマウスにおけるBVH-3-M遺伝子産物に対するウサギ抗体又は抗-BVH-11/BVH-11-2特異性Mabsの受動的伝達の保護効果
0005051959.tif 000028
【００８４】
要するに、表７及び表８の結果は、BVH-3MのようなBVH-3遺伝子産物による動物の免疫化が、実験的な菌血症及び肺炎感染を阻止可能な保護抗体を誘発したことを明白に立証している。
【００８５】
腹水を注射したマウスについて得られた保護データは下表９に示される。0.2mlの数セットの腹水の体積0.2mlの投与は、実験的感染による死を阻止した。例えば、H112-3A4 + H112-10G9及びH112-10G2 + H112-10D7セットの２種のMabs は、実験的感染に対して完全な保護を与えた。これらデータは、BVH-11及び／又はBVH-11-2エピトープを標的にする抗体が効率的な保護を与えることを示した。Mabs H112-3A4、H112-10G9、H112-10D7、H112-10A2、H112-3E8、H112-10C5、H11B-11B8、H11B-15G2、H11B-1C9、H11B-7E11、H11B-13D5及びH11-10B8が、少なくとも１つの保護Mabs対内に存在し、かつ保護エピトープに対して保護的かつ反応的であると言われた。保護供与エピトープのBVH-11-2分子上の位置は、表10及び図29にまとめられている。保護Mabs H112-3A4、H112-10G9、H112-10D7、H112-10A2、H112-3E8、H112-10C5、H11B-11B8、H11B-15G2、H11B-1C9、H11B-7E11、H11B-13D5及びH11-10B8は、すべてBVH-11-2分子上のアミノ酸残基271〜838に対応するNew10タンパク質と反応性だった。これら12Mabsのうち６種は、NEW19タンパク質内に存在するエピトープに対して向けられ、３種の保護MabsはNEW14を認識した。興味深いことに、Mab H112-3A4及びH112-10C5は、アミノ酸残基769と837の間に含まれるカルボキシル末端に位置するBVH-11-2に対して排他的な別個のエピトープと反応した。また、肺炎球菌BVH-3、BVH-11及びBVH-11-2分子によって共有されるエピトープと反応性のMabs H11-7G11、H11-6E7及びH3-4F9は、保護H112-10G9又はH112-11B8 Mabと組合せて与えた場合でさえ保護に成功しなかった。これらMabsは、それぞれ最初の225、228及び226アミノ酸残基を含むBVH-3、BVH-11及びBVH-11-2分子のアミノ末端に位置するエピトープを認識した。BVH-3、BVH-11及びBVH-11-2タンパク質配列の比較から、多数のアミノ酸が、これら225-228残基を含むアミノ末端部分内で、全体的に72.8％の同一性で保存されることが明らかになった（図32）。
【００８６】
要するに、表９及び表10に示されるデータは、保護誘発BVH-11-又はBVH-11-2-エピトープが、それぞれBVH-11及びBVH-11-2タンパク質上のアミノ酸229〜840及び227〜838を含有するカルボキシ末端産物内に含まれることを示唆している。
【００８７】
表９．BVH-11-及び／又はBVH-11-2-特異性Mabsによる受動的免疫化は、致死実験的肺炎球菌感染からマウスを保護できる。
0005051959.tif 000029
S.ニュモニエWU2による静脈内誘発４時間前に全体で0.2mlの腹水をCBA/Nマウスに腹腔内注射した。
【００８８】
表10. BVH-11-2分子上における保護を与えるエピトープの誘導される位置
0005051959.tif 000030
【００８９】
要するに、この実施例で示されるデータは、S.ニュモニエ病の予防、診断又は治療のための療法手段として、BVH-3、BVH-11又はBVH-11-2分子に対して産生される抗体の潜在的用途を実証している。
【００９０】
実施例６
この実施例は、実施例１で述べたMabを用いた表面露出ペプチドドメインの局在化について述べる。
S.ニュモニエ３型株WU2は、0.5％酵母エキス(Difco Laboratories)で富化したTodd Hewitt (TH)ブロス(Difco Laboratories, Detroit MI)内、37℃で８％CO2雰囲気で成長させ、0.260のOD600を得た(〜108 CFU/ml)。この細菌懸濁液を１ml試料に等分し、S.ニュモニエ細胞を遠心分離でペレットにし、ハイブリドーマ培養上清内で再懸濁させた。この細菌緩衝液を２時間４℃でインキュベートした。試料を遮断緩衝液[２％のウシ血清アルブミン(BSA)を含有するPBS]内で２回洗浄してから、遮断緩衝液内で希釈された１mlのヤギフルオレッセイン(FITC)が接合した抗-マウスIgG+IgMを添加した。さらに室温での60分インキュベーション後、試料を遮断緩衝液内で２回洗浄し、PBS緩衝液中の0.25％ホルムアルデヒドで18〜24時間４℃で固定した。細胞をPBS緩衝液内で１回洗浄し、500μlのPBS緩衝液に再懸濁させた。フローサイトメトリー(Epics(登録商標) XL; Beckman Coulter, Inc.)で解析するまで、細胞は暗所で４℃に保持した。１万(10,000)個の細胞を試料ごとに解析し、結果を％蛍光及び蛍光指数(FI)値として示した。％蛍光は、フルオレッセイン標識化S.ニュモニエ細胞を100で除した数であり、FI値は、Mab上清で処理した肺炎球菌の蛍光値を、接合体のみ又は対照の無関係なMabで処理した肺炎球菌の蛍光値で除した平均値である。FI値１は、該Mabが細菌の表面で検出されなかったことを指すのに対し、２より高いFI値は、少なくとも10％の肺炎球菌細胞が標識化されているときにポジティブと考えられ、該Mabは細胞表面露出エピトープと反応性であることを示した。下表11には、フローサイトメトリーで試験したMabsで得られたデータがまとめれれている。
【００９１】
フローサイトメトリー解析は、BVH-3C及び／又はBVH-11A分子と反応性のMabsが細胞表面に結合しないことを明らかにした。対照的に、Mabs H3V-9C6及びH3V-16A7を除き、NEW 1、NEW 2、NEW 3、NEW 22又はNEW 23 BVH-3遺伝子産物と反応性のMabsは、肺炎球菌の表面で検出された。これらデータは、BVH-3のアミノ末端に位置する最初の225アミノ酸残基は内部にあることを示した。Mabs H3V-9C6 及びH3V-16A7の結合性の欠如は、該配列の、S.ニュモニエSP63のBVH-3分子から欠けている177アミノ酸に対応するいくつかの部分が、抗体にアクセスできないようであることを示唆している。
【００９２】
BVH-11-及び／又はBVH-11-2-反応性Mabsの結果は、表面露出と保護との間に良い関係があることを明らかにした。フローサイトメトリー分析で決定されるように内部エピトープと反応性の全Mabsは保護的でないのに対し、実施例５で述べたすべての保護的Mabsは、フローサイトメトリーで正のシグナルを与えた。9.0のFI値及び81.2の％蛍光がMab H11-7G11で得られたが、このMabは保護を示さなかった。追加分析を行い、このMab及びその対応エピトープが抗-感染性免疫に関与するかどうかをさらに評価することができる。
【００９３】
表11．フローサイトメトリー解析によるS.ニュモニエの表面におけるMabsの結合性から得られた結果
0005051959.tif 000031
0005051959.tif 000032
【００９４】
実施例７
この実施例は、BVH-3及びBVH-11-2のペプチドエピトープによる動物の免疫化について述べる。
Mab 3A4-エピトープ(配列番号:24)をコードするDNA断片(配列番号:５上のヌクレオチド2421〜2626)を含有する組換えpSCREEN-Tベクター(Novagen, Madison, WI)を、エレクトロポレーション(Gene Pulser II apparatus, BIO-RAD Labs, Mississauga, Canada)で大腸菌Tuner(λDE3)pLysS[BL21(F'ompT hsdSB(rB-mB-) gal dcm lacYI pLysS (Cmr)](Novagen)に形質転換した。この株では、融合タンパク質の発現は、T7 RNAポリメラーゼ(λDE3プロファージ上に存在し、イソプロピル-β-D-チオガラクトピラノシド(IPTG)で誘導可能なlacプロモーターの制御下のそれ自体)によって認識されるT7プロモーターで制御される。pLysSプラスミドは、T7 RNAポリメラーゼの天然インヒビターであるT7リゾチームのコーディングによる基本的な融合タンパク質発現レベルを減少させる。
【００９５】
50mM ブドウ糖、100μg/ml カルベニシリン及び34μg/ml クロラムフェニコールで補充したLBブロス(ペプトン 10g/l、酵母エキス 5g/l、NaCl 5g/l)内37℃で、250RPMで撹拌しながら、600nmにおける吸収が0.7の値に達するまで形質転換体を成長させた。そして、IPTGを最終濃度１mMまで添加し、T7gene10タンパク質-His・Tag-3A4.1融合タンパク質の過剰発現を誘発し、さらに250RPMで撹拌しながら、25℃で３時間インキュベーションを行った。800mlの培養から誘発された細胞を遠心分離でペレットにし、-70℃で凍結させた。６ヒスチジン残基配列(His-Tag)の、金属キレーションNi-NTA樹脂(Qiagen, Mississauga, Canada)上に固定化された二価カチオン(Ni2+)に対する結合性に基づくアフィニティクロマトグラフィーによって、可溶性細胞フラクションから融合タンパク質を精製した。要するに、ペレット化細胞を解凍し、トリス緩衝ショ糖溶液(50mM トリス、25％(w/v)ショ糖)に再懸濁させ、-70℃で15分間凍結させた。細胞を15分間氷上で2mg/mlリゾチームの存在下インキュベート後、超音波処理で破壊した。このライセートを12000 RPMで30分間遠心分離し、100RPMで撹拌しながら４℃で一晩中インキュベーションした上清にニッケル荷電Ni-NTA樹脂(QIAgen)を添加した。20mMトリス、500mM NaCl、20mMイミダゾールから成るpH 7.9の緩衝液で樹脂を洗浄後、融合3A4.1タンパク質を、250mMイミダゾールで補充した同一の緩衝液で溶出した。塩及びイミダゾールの除去は、４℃でPBSに対する透析によって行った。タンパク質濃度はBCAタンパク質分析試薬キット(Perce, Rockford,IL)で決定し、760μg/mlに調整した。
【００９６】
エピトープペプチド配列による免疫化が病気に対する防御を与えうるかを評価するため、６匹のメスCBA/N (xid) マウス(National Cancer Institute)のグループ を、アフィニティ精製したT7gene10タンパク質-His・Tag-3A4.1融合タンパク質で３週間の間隔をおいて３回皮下誘発し、又は対照としてPBS中QuilAアジュバントのみを皮下注射した。３回目の免疫化後12〜14日で、マウスをS.ニュモニエWU2株で皮下誘発するか又はP4241株で鼻腔内誘発する。S.ニュモニエ誘発種菌をチョコレート寒天プレート上に塗布してCFUの数を測定し、誘発用量を検証した。誘発用量は約300CFUである。14日間毎日死亡が記録され、誘発後14日に、生存マウスを犠牲にし、血液試料をS.ニュモニエ生体の存在について試験した。3A4.1タンパク質又は試験した他のタンパク質は、感染に生き残っているマウスの数又は死亡までの平均日数が、対照の偽免疫化グループに比し、3A4.1-免疫化グループ内で有意に大きい場合に、保護的と言われる。
【００９７】
実施例８
この実施例は、BVH-3断片及びその変異体を用いた肺炎球菌に対する抗体応答の改善について述べる。
実施例２、３及び６で提示した不完全な遺伝子産物、エピトープ発現コロニー及び生きている無傷肺炎球菌とのMab反応性の研究から得られた結果を合わせると、表面露出エピトープと内部エピトープとを線引きすることができる。効率的に肺炎球菌細胞に結合されるMabsによって検出されるエピトープは、配列番号６に示されるBVH-3のアミノ酸残基223〜1039間に含まれる領域に認められた。BVH-3-カルボキシルハーフ内の保護エピトープの存在は、NEW1分子で免疫化したマウスがP4241株による致死的感染から保護されることを実証することで確認された。
【００９８】
遺伝子配列の比較から、いくつかの株では、配列番号６に示されるように、アミノ酸244〜420をコードするBVH-3の領域を欠くので、該株によって引き起こされる病気を阻止するためのワクチンにおけるこの配列の有用性は欠如していることを示唆している（配列番号:９対配列番号:１）。さらに、BVH-3断片又はその変異体は、偏在する表面露出保護エピトープに対する免疫応答を目標とする普遍的な高効率のワクチンを開発する目的で設計された。NEW1（配列番号:６からアミノ酸残基472〜1039をコードする）及びNEW40（配列番号:６からアミノ酸残基408〜1039をコードする）と命名されるBVH-3遺伝子断片は、適切な遺伝子断片の増幅のために操作されたオリゴヌクレオチドの対を用いてPCRによりS.ニュモニエ株SP64から増幅した。各プライマーは５'末端に制限エンドヌクレアーゼ部位を有し、その結果として増幅産物の消化プラスミドベクターへの方向性インフレームクローニングが可能になった。PCR増幅産物を制限エンドヌクレアーゼで消化し、同様に消化された線状プラスミドpET21（Novagen）発現ベクターに結合した。オリゴヌクレオチドプライマーHAMJ489(ccgaattccatatgcaaattgggcaaccgactc; NdeI)及びHAMJ279 (cgccaagcttcgctatgaaatcagataaattc; HindIII)をNEW40構成に使用した。クローンを、まず大腸菌DH5α内で安定化後、不完全な遺伝子産物の発現用大腸菌BL21(λDE3)中に導入した。NEW1及びNEW40由来の変異体は、Stratagene製のQuickchange Site-Directed Mutagenesisキット及び適切な突然変異を取り込むように設計されたオリゴヌクレオチドを用いて突然変異で得た。組換え分子のＣ末端上の６ヒスチジンtagの存在が、ニッケルクロマトグラフィーによる該タンパク質の精製を単純にした。下表12及び13は、それぞれ突然変異実験に用いたプライマーの配列及び生成された変異遺伝子産物を示す。プライマーセット39、40、46、47又は48を用いた突然変異実験の結果はサイレント変化となり、発現の過程で、BVH-3遺伝子と、より短い二次翻訳開始産物の断片とが共発現されることが観察されたので、所望の遺伝子又は遺伝子断片の発現を向上させる目的で行った。
【００９９】
表12．BVH-3不完全遺伝子についての部位特異的突然変異で用いられるPCRオリゴヌクレオチドプライマーセットのリスト
0005051959.tif 000033
0005051959.tif 000034
【０１００】
表13. S.ニュモニエSP64から産生された不完全な変異BVH-3遺伝子産物のリスト
0005051959.tif 000035
*下線を付したアミノ酸残基は、タンパク質配列の修飾を示す。ヌクレオチド／アミノ酸残基は、NEW105、NEW106及びNEW107構成物で欠失されている。
**サイレント突然変異、すなわちポリペプチドはNew1と同一である。
【０１０１】
実施例１で前述したように免疫化した７又は８匹のメスBALB/cマウス(Charles River)を病原性S.ニュモニエP4241株による鼻腔内誘発に対する保護実験に使用した。マウスを感染後10〜14日間観察した。表15のデータは、明らかにNEW35A分子が親NEW1と保護という点で同等であることを示している。興味深いことに、NEW40-及びNEW56-免疫化グループでは、８匹の動物のうち７及び８匹が生存するという高い生存率が得られた。同様に、アミノ酸残基233〜1039を含むNEW25が、致死的感染から８匹の動物のうち７匹を保護した。
【０１０２】
表14. 実験的肺炎におけるBVH-3断片又はその変異体によって媒介される保護
0005051959.tif 000036
【０１０３】
さらに、ワクチン接種動物由来血清抗体の結合能力のフローサイトメトリー解析は、NEW40及びNEW56抗体が、BVH-3Mに対して産生される抗体よりも効率的に生きている無傷の肺炎球菌を標識することを明かにした(表15)。
【０１０４】
表15. フローサイトメトリーで測定されたS.ニュモニエ３型株WU2の表面におけるマウス血清抗体の結合性
0005051959.tif 000037
* nd: 行わず
【０１０５】
サイトメトリーの結果は、蛍光指数値として表され、この蛍光指数は、試験血清で処理した肺炎球菌の平均蛍光値をフルオレッセイン接合体のみで処理した肺炎球菌のバックグラウンド蛍光値で除した値である。これらフローサイトメトリー分析では、全血清を１:50の希釈で使用し、BVH-3C断片又はQuilaアジュバントのみで免疫化したマウス由来の血清はバックグラウンド値と同様の値を与えた。
要するに、保護及び肺炎球菌の抗体結合性データは、NEW1又はNEW40分子及びその変異体を用いたワクチン接種が、保存されている保護的な表面露出エピトープへの免疫応答を方向づけることを示している。
【０１０６】
実施例９
この実施例は、すべてではないにしても多くのS.ニュモニエ株に存在するBVH-11-2保存保護的表面露出エピトープに対応するキメラエピトープをコードするキメラ欠失(deletant)BVH-11-2遺伝子のクローニング及び発現について述べる。
S.ニュモニエ株SP64 BVH-11-2遺伝子由来のヌクレオチド1662〜1742、1806〜2153、2193〜2414及び2484〜2627にわたる配列番号:５から生じる断片を増幅するように操作されたオリゴヌクレオチドの対を用いるPCRによって、４つの遺伝子領域に対応するBVH-11-2遺伝子断片を増幅した。
【０１０７】
使用したプライマーHAMJ490-491、HAMJ492-HAMJ493、HAMJ494-HAMJ495、HAMJ496-HAMJ354は、５'末端に制限エンドヌクレアーゼ部位を有し、その結果として増幅産物の消化pET21b(+)プラスミドベクターへの方向性インフレームクローニングが可能になった（表16）。PCR-増幅産物を制限エンドヌクレアーゼで消化し、プライマー対HAMJ490-HAMJ491で得られたPCR増幅断片を除き、同様に消化された線状プラスミドpSL301ベクターに結合した。HAMJ490-HAMJ491 PCR-増幅産物は、QIAgen (Chatsworth, CA)製のQIAquickゲル抽出キットを用いてアガロースゲルから精製し、何ら前もって制限エンドヌクレアーゼ消化もせずにpGEM-Tプラスミドに結合した。生成したプラスミド構成物をヌクレオチド配列解析によって確認した。各４種を含有する組換えプラスミドを、PCR増幅に使用される各プライマー対の５'末端と対応する制限エンドヌクレアーゼで消化した。断片を前述したようにアガロースゲルから精製し、すべてBVH11-2遺伝子の４種の領域のインフレームクローニング用制限酵素NdeI及びHindIIIで消化された線状プラスミドpET21b(+)に結合した。クローンをまず大腸菌DH5α上で安定化後、キメラ肺炎球菌タンパク質分子の発現用の大腸菌BL21(λDE3)中に導入した。
生成したNEW43遺伝子配列(配列番号:257)は図33に示される。
NEW43タンパク質(配列番号:258)の推定アミノ酸配列は図34に示される。
【０１０８】
表16. NEW43、VP43S及びNEW86の構成に使用したPCRオリゴヌクレオチドプライマーのリスト
0005051959.tif 000038
【０１０９】
表17.  NEW43の構成のためS.ニュモニエSP64から生成された不完全なBVH-11-2遺伝子断片のリスト
0005051959.tif 000039
【０１１０】
表18. NEW43遺伝子から生成されたNEW86及びVP43S遺伝子の特性
0005051959.tif 000040
【０１１１】
VP43S及びNEW86と命名されたNEW43-誘導分子は、表16及び18に記載されるPCRプライマーとpET21発現プラスミドベクターを用いて遺伝子増幅及びクローニング実験から生成された。NEW43由来の変異体は、 Stratagene製Quickchange部位特異的突然変異キット及び適切な突然変異を取り込むように設計されたオリゴヌクレオチドを用いて突然変異により生成された。組換え分子のＣ末端上の６ヒスチジンtagの存在が、ニッケルクロマトグラフィーによる該タンパク質の精製を単純にした。下表19及び20は、それぞれ突然変異実験に使用したプライマーの配列及び生成されたNEW43変異遺伝子産物を示す。
【０１１２】
表19. NEW43遺伝子についての部位特異的突然変異で用いたPCRオリゴヌクレオチドプライマーセットのリスト
0005051959.tif 000041
【０１１３】
表20. S.ニュモニエSP64から生成されたNEW43変異遺伝子産物のリスト
0005051959.tif 000042
* 下線を付したアミノ酸残基は、タンパク質配列の修飾を表す。ヌクレオチド/アミノ酸残基は、NEW88D1、NEW88D2及びNEW88構成物では欠失されている。
【０１１４】
実施例１で前述したように免疫化した７又は８匹のメスBALB/cマウス(Charles River)を、病原性S.ニュモニエP4241株による鼻腔内誘発に対する保護実験に使用した。表21のデータは、明らかにNEW19、NEW43及びその変異体が実験的肺炎に対して保護することを示している。
【０１１５】
表21. 実験的肺炎におけるNEW19及びNEW43断片又はその変異体によって媒介される保護
0005051959.tif 000043
【０１１６】
実施例１０
この実施例は、カルボキシル末端又はアミノ末端でのNEW43又はその変異体に対する融合におけるBVH-3又はその変異体のカルボキシ末端領域に対応するキメラタンパク質をコードするキメラ遺伝子のクローニング及び発現について述べる。
BVH-3の不完全な変異遺伝子及びNEW43又はNEW43変異遺伝子を含むキメラ遺伝子は、実施例１で述べた手順に従って設計した。これらキメラ遺伝子によってコードされるポリペプチドは、表22にリストされている。要するに、プライマーが５'末端に制限エンドヌクレアーゼを有するように操作されたオリゴヌクレオチドの対を用いてPCRにより、キメラ遺伝子に包含されるべき遺伝子断片が増幅され、その結果として該増幅産物の消化プラスミドベクターへの方向性インフレームクローニングが可能になった（表23及び表24）。PCR増幅産物を制限エンドヌクレアーゼで消化し、線状プラスミドpSL301ベクターに結合した。生成したプラスミド構成物をヌクレオチド配列解析で確認した。PCR産物を含有する組換えpSL301プラスミドを同一のエンドヌクレアーゼ制限酵素で再消化してDNA挿入断片を得た。結果として生じたDNA挿入断片を精製し、所定のキメラ遺伝子に対応する挿入断片をキメラ遺伝子生成用pURV22-NdeIベクターに結合した。発現された組換えタンパク質は、複数のクロマトグラフ的精製工程を用い、超音波処理した熱誘導大腸菌培養の遠心分離から得られた上清フラクションから精製した。
【０１１７】
表22. BVH-3の不完全な変異遺伝子及びNEW43又はNEW43変異遺伝子を含むキメラ遺伝子によってコードされるポリペプチドのリスト
0005051959.tif 000044
*  キメラに対してコードされたアミノ酸が遺伝子産物として現され、追加のアミノ酸残基が加えられた。Ｍはメチオニン、Ｇはグリシン、Ｐはプロリンである。
【０１１８】
表23. 表22にリストされたポリペプチドをコードするキメラ遺伝子生成用に設計されたPCRオリゴヌクレオチドプライマー対のリスト
0005051959.tif 000045
【０１１９】
表24. 表22にリストされたポリペプチドをコードするキメラ遺伝子生成用に設計されたPCRオリゴヌクレオチドプライマーのリスト
0005051959.tif 000046
【０１２０】
【配列表】
SEQUENCE LISTING
<110> SHIRE BIOCHEM INC.
HAMEL, Josee
OUELLET, Catherine
CHARLAND, Nathalie
MARTIN, Denis
BRODEUR, Bernard
<120> STREPTOCOCCUS ANTIGENS
<130> 12806-19PCT
<140> PCT/CA01/00908
<141> 2001-06-19
<150> US 60/212,683
<151> 2000-06-20
<160> 384
<170> FastSEQ for Windows Version 4.0
<210> 1
<211> 3120
<212> DNA
<213> BVH-3
<400> 1
atgaaattta gtaaaaaata tatagcagct ggatcagctg ttatcgtatc cttgagtcta      60
tgtgcctatg cactaaacca gcatcgttcg caggaaaata aggacaataa tcgtgtctct     120
tatgtggatg gcagccagtc aagtcagaaa agtgaaaact tgacaccaga ccaggttagc     180
cagaaagaag gaattcaggc tgagcaaatt gtaatcaaaa ttacagatca gggctatgta     240
acgtcacacg gtgaccacta tcattactat aatgggaaag ttccttatga tgccctcttt     300
agtgaagaac tcttgatgaa ggatccaaac tatcaactta aagacgctga tattgtcaat     360
gaagtcaagg gtggttatat catcaaggtc gatggaaaat attatgtcta cctgaaagat     420
gcagctcatg ctgataatgt tcgaactaaa gatgaaatca atcgtcaaaa acaagaacat     480
gtcaaagata atgagaaggt taactctaat gttgctgtag caaggtctca gggacgatat     540
acgacaaatg atggttatgt ctttaatcca gctgatatta tcgaagatac gggtaatgct     600
tatatcgttc ctcatggagg tcactatcac tacattccca aaagcgattt atctgctagt     660
gaattagcag cagctaaagc acatctggct ggaaaaaata tgcaaccgag tcagttaagc     720
tattcttcaa cagctagtga caataacacg caatctgtag caaaaggatc aactagcaag     780
ccagcaaata aatctgaaaa tctccagagt cttttgaagg aactctatga ttcacctagc     840
gcccaacgtt acagtgaatc agatggcctg gtctttgacc ctgctaagat tatcagtcgt     900
acaccaaatg gagttgcgat tccgcatggc gaccattacc actttattcc ttacagcaag     960
ctttctgctt tagaagaaaa gattgccaga atggtgccta tcagtggaac tggttctaca    1020
gtttctacaa atgcaaaacc taatgaagta gtgtctagtc taggcagtct ttcaagcaat    1080
ccttcttctt taacgacaag taaggagctc tcttcagcat ctgatggtta tatttttaat    1140
ccaaaagata tcgttgaaga aacggctaca gcttatattg taagacatgg tgatcatttc    1200
cattacattc caaaatcaaa tcaaattggg caaccgactc ttccaaacaa tagtctagca    1260
acaccttctc catctcttcc aatcaatcca ggaacttcac atgagaaaca tgaagaagat    1320
ggatacggat ttgatgctaa tcgtattatc gctgaagatg aatcaggttt tgtcatgagt    1380
cacggagacc acaatcatta tttcttcaag aaggacttga cagaagagca aattaaggct    1440
gcgcaaaaac atttagagga agttaaaact agtcataatg gattagattc tttgtcatct    1500
catgaacagg attatccagg taatgccaaa gaaatgaaag atttagataa aaaaatcgaa    1560
gaaaaaattg ctggcattat gaaacaatat ggtgtcaaac gtgaaagtat tgtcgtgaat    1620
aaagaaaaaa atgcgattat ttatccgcat ggagatcacc atcatgcaga tccgattgat    1680
gaacataaac cggttggaat tggtcattct cacagtaact atgaactgtt taaacccgaa    1740
gaaggagttg ctaaaaaaga agggaataaa gtttatactg gagaagaatt aacgaatgtt    1800
gttaatttgt taaaaaatag tacgtttaat aatcaaaact ttactctagc caatggtcaa    1860
aaacgcgttt cttttagttt tccgcctgaa ttggagaaaa aattaggtat caatatgcta    1920
gtaaaattaa taacaccaga tggaaaagta ttggagaaag tatctggtaa agtatttgga    1980
gaaggagtag ggaatattgc aaactttgaa ttagatcaac cttatttacc aggacaaaca    2040
tttaagtata ctatcgcttc aaaagattat ccagaagtaa gttatgatgg tacatttaca    2100
gttccaacct ctttagctta caaaatggcc agtcaaacga ttttctatcc tttccatgca    2160
ggggatactt atttaagagt gaaccctcaa tttgcagtgc ctaaaggaac tgatgcttta    2220
gtcagagtgt ttgatgaatt tcatggaaat gcttatttag aaaataacta taaagttggt    2280
gaaatcaaat taccgattcc gaaattaaac caaggaacaa ccagaacggc cggaaataaa    2340
attcctgtaa ccttcatggc aaatgcttat ttggacaatc aatcgactta tattgtggaa    2400
gtacctatct tggaaaaaga aaatcaaact gataaaccaa gtattctacc acaatttaaa    2460
aggaataaag cacaagaaaa ctcaaaactt gatgaaaagg tagaagaacc aaagactagt    2520
gagaaggtag aaaaagaaaa actttctgaa actgggaata gtactagtaa ttcaacgtta    2580
gaagaagttc ctacagtgga tcctgtacaa gaaaaagtag caaaatttgc tgaaagttat    2640
gggatgaagc tagaaaatgt cttgtttaat atggacggaa caattgaatt atatttacca    2700
tcaggagaag tcattaaaaa gaatatggca gattttacag gagaagcacc tcaaggaaat    2760
ggtgaaaata aaccatctga aaatggaaaa gtatctactg gaacagttga gaaccaacca    2820
acagaaaata aaccagcaga ttctttacca gaggcaccaa acgaaaaacc tgtaaaacca    2880
gaaaactcaa cggataatgg aatgttgaat ccagaaggga atgtggggag tgaccctatg    2940
ttagatccag cattagagga agctccagca gtagatcctg tacaagaaaa attagaaaaa    3000
tttacagcta gttacggatt aggcttagat agtgttatat tcaatatgga tggaacgatt    3060
gaattaagat tgccaagtgg agaagtgata aaaaagaatt tatctgattt catagcgtaa    3120
<210> 2
<211> 5048
<212> DNA
<213> BVH-3
<400> 2
aattccttgt cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg gcactgtctc      60
aacgagagac tcggtgaaat tttagtacct gtgaagatgc aggttacccg cgacaggacg     120
gaaagacccc atggagcttt actgcagttt gatattgagt gtctgtacca catgtacagg     180
ataggtagga gtctaagaga tcgggacgcc agtttcgaag gagacgctgt tgggatacta     240
cccttgtgtt atggccactc taacccagat aggtgatccc tatcggagac agtgtctgac     300
gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca aaggttccct     360
cagaatggtt ggaaatcatt cgcagagtgt aaaggtataa gggagcttga ctgcgagagc     420
tacaactcga gcagggacga aagtcgggct tagtgatccg gtggttccgt atggaagggc     480
catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc caagagttca     540
catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg     600
tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc agaacgtcgt     660
gagacagttc ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc tgctcctagt     720
acgagaggac cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa aggcatcgct     780
gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaaa cccacctcaa     840
gatgagattt cccatgatta tatatcagta agagccctga gagatgatca ggtagatagg     900
ttagaagtgg aagtgtggcg acacatgtag cggactaata ctaatagctc gaggacttat     960
ccaaagtaac tgagaatatg aaagcgaacg gttttcttaa attgaataga tattcaattt    1020
tgagtaggta ttactcagag ttaagtgacg atagcctagg agatacacct gtacccatgc    1080
cgaacacaga agttaagccc tagaacgccg gaagtagttg ggggttgccc cctgtgagat    1140
agggaagtcg cttagctcta gggagtttag ctcagctggg agagcatctg ccttacaagc    1200
agagggtcag cggttcgatc ccgttaactc ccaaaggtcc cgtagtgtag cggttatcac    1260
gtcgccctgt cacggcgaag atcgcgggtt cgattcccgt cgggaccgtt taaggtaacg    1320
caagttattt tagactcgtt agctcagttg gtagagcaat tgacttttaa tcaatgggtc    1380
actggttcga gcccagtacg ggtcatatat gcgggtttgg cggaattcta atctctttga    1440
aatcatcttc tctcactttc caaaactcta ttacctctta ttataccaca tttcaatctt    1500
caacttccca gtaatataag cacctctggc gaaagaagtt tcaatgtcct aaagtaataa    1560
gtgaatccaa ttcaggaact ccaagaacaa aagaaacatc tggtgtcaca agtattggat    1620
ggcacagagt cacgtggtag tctgacccta gcagaaattt taaatagtaa actatttact    1680
ggttaattaa atggttaaat aaccggttta gaaaactatt taataaagta aaagaagttg    1740
agaaaaaact tcatcattta ttgaaatgag ggatttatga aatttagtaa aaaatatata    1800
gcagctggat cagctgttat cgtatccttg agtctatgtg cctatgcact aaaccagcat    1860
cgttcgcagg aaaataagga caataatcgt gtctcttatg tggatggcag ccagtcaagt    1920
cagaaaagtg aaaacttgac accagaccag gttagccaga aagaaggaat tcaggctgag    1980
caaattgtaa tcaaaattac agatcagggc tatgtaacgt cacacggtga ccactatcat    2040
tactataatg ggaaagttcc ttatgatgcc ctctttagtg aagaactctt gatgaaggat    2100
ccaaactatc aacttaaaga cgctgatatt gtcaatgaag tcaagggtgg ttatatcatc    2160
aaggtcgatg gaaaatatta tgtctacctg aaagatgcag ctcatgctga taatgttcga    2220
actaaagatg aaatcaatcg tcaaaaacaa gaacatgtca aagataatga gaaggttaac    2280
tctaatgttg ctgtagcaag gtctcaggga cgatatacga caaatgatgg ttatgtcttt    2340
aatccagctg atattatcga agatacgggt aatgcttata tcgttcctca tggaggtcac    2400
tatcactaca ttcccaaaag cgatttatct gctagtgaat tagcagcagc taaagcacat    2460
ctggctggaa aaaatatgca accgagtcag ttaagctatt cttcaacagc tagtgacaat    2520
aacacgcaat ctgtagcaaa aggatcaact agcaagccag caaataaatc tgaaaatctc    2580
cagagtcttt tgaaggaact ctatgattca cctagcgccc aacgttacag tgaatcagat    2640
ggcctggtct ttgaccctgc taagattatc agtcgtacac caaatggagt tgcgattccg    2700
catggcgacc attaccactt tattccttac agcaagcttt ctgctttaga agaaaagatt    2760
gccagaatgg tgcctatcag tggaactggt tctacagttt ctacaaatgc aaaacctaat    2820
gaagtagtgt ctagtctagg cagtctttca agcaatcctt cttctttaac gacaagtaag    2880
gagctctctt cagcatctga tggttatatt tttaatccaa aagatatcgt tgaagaaacg    2940
gctacagctt atattgtaag acatggtgat catttccatt acattccaaa atcaaatcaa    3000
attgggcaac cgactcttcc aaacaatagt ctagcaacac cttctccatc tcttccaatc    3060
aatccaggaa cttcacatga gaaacatgaa gaagatggat acggatttga tgctaatcgt    3120
attatcgctg aagatgaatc aggttttgtc atgagtcacg gagaccacaa tcattatttc    3180
ttcaagaagg acttgacaga agagcaaatt aaggctgcgc aaaaacattt agaggaagtt    3240
aaaactagtc ataatggatt agattctttg tcatctcatg aacaggatta tccaggtaat    3300
gccaaagaaa tgaaagattt agataaaaaa atcgaagaaa aaattgctgg cattatgaaa    3360
caatatggtg tcaaacgtga aagtattgtc gtgaataaag aaaaaaatgc gattatttat    3420
ccgcatggag atcaccatca tgcagatccg attgatgaac ataaaccggt tggaattggt    3480
cattctcaca gtaactatga actgtttaaa cccgaagaag gagttgctaa aaaagaaggg    3540
aataaagttt atactggaga agaattaacg aatgttgtta atttgttaaa aaatagtacg    3600
tttaataatc aaaactttac tctagccaat ggtcaaaaac gcgtttcttt tagttttccg    3660
cctgaattgg agaaaaaatt aggtatcaat atgctagtaa aattaataac accagatgga    3720
aaagtattgg agaaagtatc tggtaaagta tttggagaag gagtagggaa tattgcaaac    3780
tttgaattag atcaacctta tttaccagga caaacattta agtatactat cgcttcaaaa    3840
gattatccag aagtaagtta tgatggtaca tttacagttc caacctcttt agcttacaaa    3900
atggccagtc aaacgatttt ctatcctttc catgcagggg atacttattt aagagtgaac    3960
cctcaatttg cagtgcctaa aggaactgat gctttagtca gagtgtttga tgaatttcat    4020
ggaaatgctt atttagaaaa taactataaa gttggtgaaa tcaaattacc gattccgaaa    4080
ttaaaccaag gaacaaccag aacggccgga aataaaattc ctgtaacctt catggcaaat    4140
gcttatttgg acaatcaatc gacttatatt gtggaagtac ctatcttgga aaaagaaaat    4200
caaactgata aaccaagtat tctaccacaa tttaaaagga ataaagcaca agaaaactca    4260
aaacttgatg aaaaggtaga agaaccaaag actagtgaga aggtagaaaa agaaaaactt    4320
tctgaaactg ggaatagtac tagtaattca acgttagaag aagttcctac agtggatcct    4380
gtacaagaaa aagtagcaaa atttgctgaa agttatggga tgaagctaga aaatgtcttg    4440
tttaatatgg acggaacaat tgaattatat ttaccatcag gagaagtcat taaaaagaat    4500
atggcagatt ttacaggaga agcacctcaa ggaaatggtg aaaataaacc atctgaaaat    4560
ggaaaagtat ctactggaac agttgagaac caaccaacag aaaataaacc agcagattct    4620
ttaccagagg caccaaacga aaaacctgta aaaccagaaa actcaacgga taatggaatg    4680
ttgaatccag aagggaatgt ggggagtgac cctatgttag atccagcatt agaggaagct    4740
ccagcagtag atcctgtaca agaaaaatta gaaaaattta cagctagtta cggattaggc    4800
ttagatagtg ttatattcaa tatggatgga acgattgaat taagattgcc aagtggagaa    4860
gtgataaaaa agaatttatc tgatttcata gcgtaaggaa tagcagtaga aaaagtctga    4920
atcaaaaatg aagttctctc aaaagttaga aataaaactc tgactttggg agaatttcat    4980
tttattatta atatataaaa tttcttgaca tacaacttaa aaagaggtgg aatatttact    5040
agttaatt                                                             5048
<210> 3
<211> 2523
<212> DNA
<213> BVH-11
<400> 3
atgaaaatca ataaaaaata tctagctggg tcagtagcta cacttgtttt aagtgtctgt      60
gcttatgaac taggtttgca tcaagctcaa actgtaaaag aaaataatcg tgtttcctat     120
atagatggaa aacaagcgac gcaaaaaacg gagaatttga ctcctgatga ggttagcaag     180
cgtgaaggaa tcaacgccga acaaatcgtc atcaagatta cggatcaagg ttatgtgacc     240
tctcatggag accattatca ttactataat ggcaaggtcc cttatgatgc catcatcagt     300
gaagagctcc tcatgaaaga tccgaattat cagttgaagg attcagacat tgtcaatgaa     360
atcaagggtg gttatgtcat taaggtaaac ggtaaatact atgtttacct taaggatgca     420
gctcatgcgg ataatgtccg tacaaaagaa gaaatcaatc ggcaaaaaca agaacatagt     480
cagcatcgtg aaggagggac ttcagcaaac gatggtgcgg tagcctttgc acgttcacag     540
ggacgctaca ccacagatga tggttatatc ttcaatgcat ctgatatcat cgaagatacg     600
ggcgatgcct atatcgttcc tcatggagat cattaccatt acattcctaa gaatgagtta     660
tcagctagcg agttggctgc tgcagaagcc ttcctatctg gtcgggaaaa tctgtcaaat     720
ttaagaacct atcgccgaca aaatagcgat aacactccaa gaacaaactg ggtaccttct     780
gtaagcaatc caggaactac aaatactaac acaagcaaca acagcaacac taacagtcaa     840
gcaagtcaaa gtaatgacat tgatagtctc ttgaaacagc tctacaaact gcctttgagt     900
caacgccatg tagaatctga tggccttatt ttcgacccag cgcaaatcac aagtcgaacc     960
gccagaggtg tagctgtccc tcatggtaac cattaccact ttatccctta tgaacaaatg    1020
tctgaattgg aaaaacgaat tgctcgtatt attccccttc gttatcgttc aaaccattgg    1080
gtaccagatt caagaccaga agaaccaagt ccacaaccga ctccagaacc tagtccaagt    1140
ccgcaacctg caccaaatcc tcaaccagct ccaagcaatc caattgatga gaaattggtc    1200
aaagaagctg ttcgaaaagt aggcgatggt tatgtctttg aggagaatgg agtttctcgt    1260
tatatcccag ccaagaatct ttcagcagaa acagcagcag gcattgatag caaactggcc    1320
aagcaggaaa gtttatctca taagctagga gctaagaaaa ctgacctccc atctagtgat    1380
cgagaatttt acaataaggc ttatgactta ctagcaagaa ttcaccaaga tttacttgat    1440
aataaaggtc gacaagttga ttttgaggct ttggataacc tgttggaacg actcaaggat    1500
gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc tccgattcgt    1560
catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga tgagattcaa    1620
gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga tcctcgtgat    1680
ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag ccactggatt    1740
aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc taaagagaaa    1800
ggtttgaccc ctccttcgac agaccatcag gattcaggaa atactgaggc aaaaggagca    1860
gaagctatct acaaccgcgt gaaagcagct aagaaggtgc cacttgatcg tatgccttac    1920
aatcttcaat atactgtaga agtcaaaaac ggtagtttaa tcatacctca ttatgaccat    1980
taccataaca tcaaatttga gtggtttgac gaaggccttt atgaggcacc taaggggtat    2040
actcttgagg atcttttggc gactgtcaag tactatgtcg aacatccaaa cgaacgtccg    2100
cattcagata atggttttgg taacgctagc gaccatgttc aaagaaacaa aaatggtcaa    2160
gctgatacca atcaaacgga aaaaccaagc gaggagaaac ctcagacaga aaaacctgag    2220
gaagaaaccc ctcgagaaga gaaaccacaa agcgagaaac cagagtctcc aaaaccaaca    2280
gaggaaccag aagaagaatc accagaggaa tcagaagaac ctcaggtcga gactgaaaag    2340
gttgaagaaa aactgagaga ggctgaagat ttacttggaa aaatccagga tccaattatc    2400
aagtccaatg ccaaagagac tctcacagga ttaaaaaata atttactatt tggcacccag    2460
gacaacaata ctattatggc agaagctgaa aaactattgg ctttattaaa ggagagtaag    2520
taa                                                                  2523
<210> 4
<211> 2647
<212> DNA
<213> BVH-11
<400> 4
cagagatctt agtgaatcaa atatacttaa gaaaagagga aagaatgaaa atcaataaaa      60
aatatctagc tgggtcagta gctacacttg ttttaagtgt ctgtgcttat gaactaggtt     120
tgcatcaagc tcaaactgta aaagaaaata atcgtgtttc ctatatagat ggaaaacaag     180
cgacgcaaaa aacggagaat ttgactcctg atgaggttag caagcgtgaa ggaatcaacg     240
ccgaacaaat cgtcatcaag attacggatc aaggttatgt gacctctcat ggagaccatt     300
atcattacta taatggcaag gtcccttatg atgccatcat cagtgaagag ctcctcatga     360
aagatccgaa ttatcagttg aaggattcag acattgtcaa tgaaatcaag ggtggttatg     420
tcattaaggt aaacggtaaa tactatgttt accttaagga tgcagctcat gcggataatg     480
tccgtacaaa agaagaaatc aatcggcaaa aacaagaaca tagtcagcat cgtgaaggag     540
ggacttcagc aaacgatggt gcggtagcct ttgcacgttc acagggacgc tacaccacag     600
atgatggtta tatcttcaat gcatctgata tcatcgaaga tacgggcgat gcctatatcg     660
ttcctcatgg agatcattac cattacattc ctaagaatga gttatcagct agcgagttgg     720
ctgctgcaga agccttccta tctggtcggg aaaatctgtc aaatttaaga acctatcgcc     780
gacaaaatag cgataacact ccaagaacaa actgggtacc ttctgtaagc aatccaggaa     840
ctacaaatac taacacaagc aacaacagca acactaacag tcaagcaagt caaagtaatg     900
acattgatag tctcttgaaa cagctctaca aactgccttt gagtcaacgc catgtagaat     960
ctgatggcct tattttcgac ccagcgcaaa tcacaagtcg aaccgccaga ggtgtagctg    1020
tccctcatgg taaccattac cactttatcc cttatgaaca aatgtctgaa ttggaaaaac    1080
gaattgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca gattcaagac    1140
cagaagaacc aagtccacaa ccgactccag aacctagtcc aagtccgcaa cctgcaccaa    1200
atcctcaacc agctccaagc aatccaattg atgagaaatt ggtcaaagaa gctgttcgaa    1260
aagtaggcga tggttatgtc tttgaggaga atggagtttc tcgttatatc ccagccaaga    1320
atctttcagc agaaacagca gcaggcattg atagcaaact ggccaagcag gaaagtttat    1380
ctcataagct aggagctaag aaaactgacc tcccatctag tgatcgagaa ttttacaata    1440
aggcttatga cttactagca agaattcacc aagatttact tgataataaa ggtcgacaag    1500
ttgattttga ggctttggat aacctgttgg aacgactcaa ggatgtctca agtgataaag    1560
tcaagttagt ggatgatatt cttgccttct tagctccgat tcgtcatcca gaacgtttag    1620
gaaaaccaaa tgcgcaaatt acctacactg atgatgagat tcaagtagcc aagttggcag    1680
gcaagtacac aacagaagac ggttatatct ttgatcctcg tgatataacc agtgatgagg    1740
gggatgccta tgtaactcca catatgaccc atagccactg gattaaaaaa gatagtttgt    1800
ctgaagctga gagagcggca gcccaggctt atgctaaaga gaaaggtttg acccctcctt    1860
cgacagacca tcaggattca ggaaatactg aggcaaaagg agcagaagct atctacaacc    1920
gcgtgaaagc agctaagaag gtgccacttg atcgtatgcc ttacaatctt caatatactg    1980
tagaagtcaa aaacggtagt ttaatcatac ctcattatga ccattaccat aacatcaaat    2040
ttgagtggtt tgacgaaggc ctttatgagg cacctaaggg gtatactctt gaggatcttt    2100
tggcgactgt caagtactat gtcgaacatc caaacgaacg tccgcattca gataatggtt    2160
ttggtaacgc tagcgaccat gttcaaagaa acaaaaatgg tcaagctgat accaatcaaa    2220
cggaaaaacc aagcgaggag aaacctcaga cagaaaaacc tgaggaagaa acccctcgag    2280
aagagaaacc acaaagcgag aaaccagagt ctccaaaacc aacagaggaa ccagaagaag    2340
aatcaccaga ggaatcagaa gaacctcagg tcgagactga aaaggttgaa gaaaaactga    2400
gagaggctga agatttactt ggaaaaatcc aggatccaat tatcaagtcc aatgccaaag    2460
agactctcac aggattaaaa aataatttac tatttggcac ccaggacaac aatactatta    2520
tggcagaagc tgaaaaacta ttggctttat taaaggagag taagtaaagg tagcagcatt    2580
ttctaactcc taaaaacagg ataggagaac gggaaaacga aaaatgagag cagaatgtga    2640
gttctag                                                              2647
<210> 5
<211> 2639
<212> DNA
<213> BVH-11-2
<400> 5
gggtcttaaa actctgaatc ctttagaggc agacccacaa aatgacaaga cctatttaga      60
aaatctggaa gaaaatatga gtgttctagc agaagaatta aagtgaggaa agaatgaaaa     120
tcaataaaaa atatctagca ggttcagtgg cagtccttgc cctaagtgtt tgttcctatg     180
aacttggtcg tcaccaagct ggtcaggtta agaaagagtc taatcgagtt tcttatatag     240
atggtgatca ggctggtcaa aaggcagaaa atttgacacc agatgaagtc agtaagagag     300
aggggatcaa cgccgaacaa attgttatca agattacgga tcaaggttat gtgacctctc     360
atggagacca ttatcattac tataatggca aggttcctta tgatgccatc atcagtgaag     420
aacttctcat gaaagatccg aattatcagt tgaaggattc agacattgtc aatgaaatca     480
agggtggcta tgtgattaag gtagacggaa aatactatgt ttaccttaaa gatgcggccc     540
atgcggacaa tattcggaca aaagaagaga ttaaacgtca gaagcaggaa cacagtcata     600
atcataactc aagagcagat aatgctgttg ctgcagccag agcccaagga cgttatacaa     660
cggatgatgg gtatatcttc aatgcatctg atatcattga ggacacgggt gatgcttata     720
tcgttcctca cggcgaccat taccattaca ttcctaagaa tgagttatca gctagcgagt     780
tagctgctgc agaagcctat tggaatggga agcagggatc tcgtccttct tcaagttcta     840
gttataatgc aaatccagtt caaccaagat tgtcagagaa ccacaatctg actgtcactc     900
caacttatca tcaaaatcaa ggggaaaaca tttcaagcct tttacgtgaa ttgtatgcta     960
aacccttatc agaacgccat gtagaatctg atggccttat tttcgaccca gcgcaaatca    1020
caagtcgaac cgccagaggt gtagctgtcc ctcatggtaa ccattaccac tttatccctt    1080
atgaacaaat gtctgaattg gaaaaacgaa ttgctcgtat tattcccctt cgttatcgtt    1140
caaaccattg ggtaccagat tcaagaccag aacaaccaag tccacaatcg actccggaac    1200
ctagtccaag tctgcaacct gcaccaaatc ctcaaccagc tccaagcaat ccaattgatg    1260
agaaattggt caaagaagct gttcgaaaag taggcgatgg ttatgtcttt gaggagaatg    1320
gagtttctcg ttatatccca gccaaggatc tttcagcaga aacagcagca ggcattgata    1380
gcaaactggc caagcaggaa agtttatctc ataagctagg agctaagaaa actgacctcc    1440
catctagtga tcgagaattt tacaataagg cttatgactt actagcaaga attcaccaag    1500
atttacttga taataaaggt cgacaagttg attttgaggt tttggataac ctgttggaac    1560
gactcaagga tgtctcaagt gataaagtca agttagtgga tgatattctt gccttcttag    1620
ctccgattcg tcatccagaa cgtttaggaa aaccaaatgc gcaaattacc tacactgatg    1680
atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt tatatctttg    1740
atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat atgacccata    1800
gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc caggcttatg    1860
ctaaagagaa aggtttgacc cctccttcga cagaccacca ggattcagga aatactgagg    1920
caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg ccacttgatc    1980
gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta atcatacctc    2040
attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt tatgaggcac    2100
ctaaggggta tagtcttgag gatcttttgg cgactgtcaa gtactatgtc gaacatccaa    2160
acgaacgtcc gcattcagat aatggttttg gtaacgctag tgaccatgtt cgtaaaaata    2220
aggcagacca agatagtaaa cctgatgaag ataaggaaca tgatgaagta agtgagccaa    2280
ctcaccctga atctgatgaa aaagagaatc acgctggttt aaatccttca gcagataatc    2340
tttataaacc aagcactgat acggaagaga cagaggaaga agctgaagat accacagatg    2400
aggctgaaat tcctcaagta gagaattctg ttattaacgc taagatagca gatgcggagg    2460
ccttgctaga aaaagtaaca gatcctagta ttagacaaaa tgctatggag acattgactg    2520
gtctaaaaag tagtcttctt ctcggaacga aagataataa cactatttca gcagaagtag    2580
atagtctctt ggctttgtta aaagaaagtc aaccggctcc tatacagtag taaaatgaa     2639
<210> 6
<211> 1039
<212> PRT
<213> BVH-3
<400> 6
Met Lys Phe Ser Lys Lys Tyr Ile Ala Ala Gly Ser Ala Val Ile Val
1               5                  10                  15
Ser Leu Ser Leu Cys Ala Tyr Ala Leu Asn Gln His Arg Ser Gln Glu
20                  25                  30
Asn Lys Asp Asn Asn Arg Val Ser Tyr Val Asp Gly Ser Gln Ser Ser
35                  40                  45
Gln Lys Ser Glu Asn Leu Thr Pro Asp Gln Val Ser Gln Lys Glu Gly
50                  55                  60
Ile Gln Ala Glu Gln Ile Val Ile Lys Ile Thr Asp Gln Gly Tyr Val
65                  70                  75                  80
Thr Ser His Gly Asp His Tyr His Tyr Tyr Asn Gly Lys Val Pro Tyr
85                  90                  95
Asp Ala Leu Phe Ser Glu Glu Leu Leu Met Lys Asp Pro Asn Tyr Gln
100                 105                 110
Leu Lys Asp Ala Asp Ile Val Asn Glu Val Lys Gly Gly Tyr Ile Ile
115                 120                 125
Lys Val Asp Gly Lys Tyr Tyr Val Tyr Leu Lys Asp Ala Ala His Ala
130                 135                 140
Asp Asn Val Arg Thr Lys Asp Glu Ile Asn Arg Gln Lys Gln Glu His
145                 150                 155                 160
Val Lys Asp Asn Glu Lys Val Asn Ser Asn Val Ala Val Ala Arg Ser
165                 170                 175
Gln Gly Arg Tyr Thr Thr Asn Asp Gly Tyr Val Phe Asn Pro Ala Asp
180                 185                 190
Ile Ile Glu Asp Thr Gly Asn Ala Tyr Ile Val Pro His Gly Gly His
195                 200                 205
Tyr His Tyr Ile Pro Lys Ser Asp Leu Ser Ala Ser Glu Leu Ala Ala
210                 215                 220
Ala Lys Ala His Leu Ala Gly Lys Asn Met Gln Pro Ser Gln Leu Ser
225                 230                 235                 240
Tyr Ser Ser Thr Ala Ser Asp Asn Asn Thr Gln Ser Val Ala Lys Gly
245                 250                 255
Ser Thr Ser Lys Pro Ala Asn Lys Ser Glu Asn Leu Gln Ser Leu Leu
260                 265                 270
Lys Glu Leu Tyr Asp Ser Pro Ser Ala Gln Arg Tyr Ser Glu Ser Asp
275                 280                 285
Gly Leu Val Phe Asp Pro Ala Lys Ile Ile Ser Arg Thr Pro Asn Gly
290                 295                 300
Val Ala Ile Pro His Gly Asp His Tyr His Phe Ile Pro Tyr Ser Lys
305                 310                 315                 320
Leu Ser Ala Leu Glu Glu Lys Ile Ala Arg Met Val Pro Ile Ser Gly
325                 330                 335
Thr Gly Ser Thr Val Ser Thr Asn Ala Lys Pro Asn Glu Val Val Ser
340                 345                 350
Ser Leu Gly Ser Leu Ser Ser Asn Pro Ser Ser Leu Thr Thr Ser Lys
355                 360                 365
Glu Leu Ser Ser Ala Ser Asp Gly Tyr Ile Phe Asn Pro Lys Asp Ile
370                 375                 380
Val Glu Glu Thr Ala Thr Ala Tyr Ile Val Arg His Gly Asp His Phe
385                 390                 395                 400
His Tyr Ile Pro Lys Ser Asn Gln Ile Gly Gln Pro Thr Leu Pro Asn
405                 410                 415
Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr
420                 425                 430
Ser His Glu Lys His Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg
435                 440                 445
Ile Ile Ala Glu Asp Glu Ser Gly Phe Val Met Ser His Gly Asp His
450                 455                 460
Asn His Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala
465                 470                 475                 480
Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp
485                 490                 495
Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met
500                 505                 510
Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys
515                 520                 525
Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn
530                 535                 540
Ala Ile Ile Tyr Pro His Gly Asp His His His Ala Asp Pro Ile Asp
545                 550                 555                 560
Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu
565                 570                 575
Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr
580                 585                 590
Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr
595                 600                 605
Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser
610                 615                 620
Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu
625                 630                 635                 640
Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly
645                 650                 655
Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp
660                 665                 670
Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys
675                 680                 685
Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser
690                 695                 700
Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala
705                 710                 715                 720
Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly
725                 730                 735
Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr
740                 745                 750
Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys
755                 760                 765
Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr
770                 775                 780
Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu
785                 790                 795                 800
Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu
805                 810                 815
Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu
820                 825                 830
Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu
835                 840                 845
Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro
850                 855                 860
Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr
865                 870                 875                 880
Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu
885                 890                 895
Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe
900                 905                 910
Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn
915                 920                 925
Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys
930                 935                 940
Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro
945                 950                 955                 960
Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly
965                 970                 975
Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp
980                 985                 990
Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly
995                 1000                1005
Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu
1010                1015                1020
Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
1025                1030                1035
<210> 7
<211> 840
<212> PRT
<213> BVH-11
<400> 7
Met Lys Ile Asn Lys Lys Tyr Leu Ala Gly Ser Val Ala Thr Leu Val
1               5                  10                  15
Leu Ser Val Cys Ala Tyr Glu Leu Gly Leu His Gln Ala Gln Thr Val
20                  25                  30
Lys Glu Asn Asn Arg Val Ser Tyr Ile Asp Gly Lys Gln Ala Thr Gln
35                  40                  45
Lys Thr Glu Asn Leu Thr Pro Asp Glu Val Ser Lys Arg Glu Gly Ile
50                  55                  60
Asn Ala Glu Gln Ile Val Ile Lys Ile Thr Asp Gln Gly Tyr Val Thr
65                  70                  75                  80
Ser His Gly Asp His Tyr His Tyr Tyr Asn Gly Lys Val Pro Tyr Asp
85                  90                  95
Ala Ile Ile Ser Glu Glu Leu Leu Met Lys Asp Pro Asn Tyr Gln Leu
100                 105                 110
Lys Asp Ser Asp Ile Val Asn Glu Ile Lys Gly Gly Tyr Val Ile Lys
115                 120                 125
Val Asn Gly Lys Tyr Tyr Val Tyr Leu Lys Asp Ala Ala His Ala Asp
130                 135                 140
Asn Val Arg Thr Lys Glu Glu Ile Asn Arg Gln Lys Gln Glu His Ser
145                 150                 155                 160
Gln His Arg Glu Gly Gly Thr Ser Ala Asn Asp Gly Ala Val Ala Phe
165                 170                 175
Ala Arg Ser Gln Gly Arg Tyr Thr Thr Asp Asp Gly Tyr Ile Phe Asn
180                 185                 190
Ala Ser Asp Ile Ile Glu Asp Thr Gly Asp Ala Tyr Ile Val Pro His
195                 200                 205
Gly Asp His Tyr His Tyr Ile Pro Lys Asn Glu Leu Ser Ala Ser Glu
210                 215                 220
Leu Ala Ala Ala Glu Ala Phe Leu Ser Gly Arg Glu Asn Leu Ser Asn
225                 230                 235                 240
Leu Arg Thr Tyr Arg Arg Gln Asn Ser Asp Asn Thr Pro Arg Thr Asn
245                 250                 255
Trp Val Pro Ser Val Ser Asn Pro Gly Thr Thr Asn Thr Asn Thr Ser
260                 265                 270
Asn Asn Ser Asn Thr Asn Ser Gln Ala Ser Gln Ser Asn Asp Ile Asp
275                 280                 285
Ser Leu Leu Lys Gln Leu Tyr Lys Leu Pro Leu Ser Gln Arg His Val
290                 295                 300
Glu Ser Asp Gly Leu Ile Phe Asp Pro Ala Gln Ile Thr Ser Arg Thr
305                 310                 315                 320
Ala Arg Gly Val Ala Val Pro His Gly Asn His Tyr His Phe Ile Pro
325                 330                 335
Tyr Glu Gln Met Ser Glu Leu Glu Lys Arg Ile Ala Arg Ile Ile Pro
340                 345                 350
Leu Arg Tyr Arg Ser Asn His Trp Val Pro Asp Ser Arg Pro Glu Glu
355                 360                 365
Pro Ser Pro Gln Pro Thr Pro Glu Pro Ser Pro Ser Pro Gln Pro Ala
370                 375                 380
Pro Asn Pro Gln Pro Ala Pro Ser Asn Pro Ile Asp Glu Lys Leu Val
385                 390                 395                 400
Lys Glu Ala Val Arg Lys Val Gly Asp Gly Tyr Val Phe Glu Glu Asn
405                 410                 415
Gly Val Ser Arg Tyr Ile Pro Ala Lys Asn Leu Ser Ala Glu Thr Ala
420                 425                 430
Ala Gly Ile Asp Ser Lys Leu Ala Lys Gln Glu Ser Leu Ser His Lys
435                 440                 445
Leu Gly Ala Lys Lys Thr Asp Leu Pro Ser Ser Asp Arg Glu Phe Tyr
450                 455                 460
Asn Lys Ala Tyr Asp Leu Leu Ala Arg Ile His Gln Asp Leu Leu Asp
465                 470                 475                 480
Asn Lys Gly Arg Gln Val Asp Phe Glu Ala Leu Asp Asn Leu Leu Glu
485                 490                 495
Arg Leu Lys Asp Val Ser Ser Asp Lys Val Lys Leu Val Asp Asp Ile
500                 505                 510
Leu Ala Phe Leu Ala Pro Ile Arg His Pro Glu Arg Leu Gly Lys Pro
515                 520                 525
Asn Ala Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu
530                 535                 540
Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Pro Arg Asp
545                 550                 555                 560
Ile Thr Ser Asp Glu Gly Asp Ala Tyr Val Thr Pro His Met Thr His
565                 570                 575
Ser His Trp Ile Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala
580                 585                 590
Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp
595                 600                 605
His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr
610                 615                 620
Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr
625                 630                 635                 640
Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro
645                 650                 655
His Tyr Asp His Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly
660                 665                 670
Leu Tyr Glu Ala Pro Lys Gly Tyr Thr Leu Glu Asp Leu Leu Ala Thr
675                 680                 685
Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg Pro His Ser Asp Asn
690                 695                 700
Gly Phe Gly Asn Ala Ser Asp His Val Gln Arg Asn Lys Asn Gly Gln
705                 710                 715                 720
Ala Asp Thr Asn Gln Thr Glu Lys Pro Ser Glu Glu Lys Pro Gln Thr
725                 730                 735
Glu Lys Pro Glu Glu Glu Thr Pro Arg Glu Glu Lys Pro Gln Ser Glu
740                 745                 750
Lys Pro Glu Ser Pro Lys Pro Thr Glu Glu Pro Glu Glu Glu Ser Pro
755                 760                 765
Glu Glu Ser Glu Glu Pro Gln Val Glu Thr Glu Lys Val Glu Glu Lys
770                 775                 780
Leu Arg Glu Ala Glu Asp Leu Leu Gly Lys Ile Gln Asp Pro Ile Ile
785                 790                 795                 800
Lys Ser Asn Ala Lys Glu Thr Leu Thr Gly Leu Lys Asn Asn Leu Leu
805                 810                 815
Phe Gly Thr Gln Asp Asn Asn Thr Ile Met Ala Glu Ala Glu Lys Leu
820                 825                 830
Leu Ala Leu Leu Lys Glu Ser Lys
835                 840
<210> 8
<211> 838
<212> PRT
<213> BVH-11-2
<400> 8
Met Lys Ile Asn Lys Lys Tyr Leu Ala Gly Ser Val Ala Val Leu Ala
1               5                  10                  15
Leu Ser Val Cys Ser Tyr Glu Leu Gly Arg His Gln Ala Gly Gln Val
20                  25                  30
Lys Lys Glu Ser Asn Arg Val Ser Tyr Ile Asp Gly Asp Gln Ala Gly
35                  40                  45
Gln Lys Ala Glu Asn Leu Thr Pro Asp Glu Val Ser Lys Arg Glu Gly
50                  55                  60
Ile Asn Ala Glu Gln Ile Val Ile Lys Ile Thr Asp Gln Gly Tyr Val
65                  70                  75                  80
Thr Ser His Gly Asp His Tyr His Tyr Tyr Asn Gly Lys Val Pro Tyr
85                  90                  95
Asp Ala Ile Ile Ser Glu Glu Leu Leu Met Lys Asp Pro Asn Tyr Gln
100                 105                 110
Leu Lys Asp Ser Asp Ile Val Asn Glu Ile Lys Gly Gly Tyr Val Ile
115                 120                 125
Lys Val Asp Gly Lys Tyr Tyr Val Tyr Leu Lys Asp Ala Ala His Ala
130                 135                 140
Asp Asn Ile Arg Thr Lys Glu Glu Ile Lys Arg Gln Lys Gln Glu His
145                 150                 155                 160
Ser His Asn His Asn Ser Arg Ala Asp Asn Ala Val Ala Ala Ala Arg
165                 170                 175
Ala Gln Gly Arg Tyr Thr Thr Asp Asp Gly Tyr Ile Phe Asn Ala Ser
180                 185                 190
Asp Ile Ile Glu Asp Thr Gly Asp Ala Tyr Ile Val Pro His Gly Asp
195                 200                 205
His Tyr His Tyr Ile Pro Lys Asn Glu Leu Ser Ala Ser Glu Leu Ala
210                 215                 220
Ala Ala Glu Ala Tyr Trp Asn Gly Lys Gln Gly Ser Arg Pro Ser Ser
225                 230                 235                 240
Ser Ser Ser Tyr Asn Ala Asn Pro Val Gln Pro Arg Leu Ser Glu Asn
245                 250                 255
His Asn Leu Thr Val Thr Pro Thr Tyr His Gln Asn Gln Gly Glu Asn
260                 265                 270
Ile Ser Ser Leu Leu Arg Glu Leu Tyr Ala Lys Pro Leu Ser Glu Arg
275                 280                 285
His Val Glu Ser Asp Gly Leu Ile Phe Asp Pro Ala Gln Ile Thr Ser
290                 295                 300
Arg Thr Ala Arg Gly Val Ala Val Pro His Gly Asn His Tyr His Phe
305                 310                 315                 320
Ile Pro Tyr Glu Gln Met Ser Glu Leu Glu Lys Arg Ile Ala Arg Ile
325                 330                 335
Ile Pro Leu Arg Tyr Arg Ser Asn His Trp Val Pro Asp Ser Arg Pro
340                 345                 350
Glu Gln Pro Ser Pro Gln Ser Thr Pro Glu Pro Ser Pro Ser Leu Gln
355                 360                 365
Pro Ala Pro Asn Pro Gln Pro Ala Pro Ser Asn Pro Ile Asp Glu Lys
370                 375                 380
Leu Val Lys Glu Ala Val Arg Lys Val Gly Asp Gly Tyr Val Phe Glu
385                 390                 395                 400
Glu Asn Gly Val Ser Arg Tyr Ile Pro Ala Lys Asp Leu Ser Ala Glu
405                 410                 415
Thr Ala Ala Gly Ile Asp Ser Lys Leu Ala Lys Gln Glu Ser Leu Ser
420                 425                 430
His Lys Leu Gly Ala Lys Lys Thr Asp Leu Pro Ser Ser Asp Arg Glu
435                 440                 445
Phe Tyr Asn Lys Ala Tyr Asp Leu Leu Ala Arg Ile His Gln Asp Leu
450                 455                 460
Leu Asp Asn Lys Gly Arg Gln Val Asp Phe Glu Val Leu Asp Asn Leu
465                 470                 475                 480
Leu Glu Arg Leu Lys Asp Val Ser Ser Asp Lys Val Lys Leu Val Asp
485                 490                 495
Asp Ile Leu Ala Phe Leu Ala Pro Ile Arg His Pro Glu Arg Leu Gly
500                 505                 510
Lys Pro Asn Ala Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala
515                 520                 525
Lys Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Pro
530                 535                 540
Arg Asp Ile Thr Ser Asp Glu Gly Asp Ala Tyr Val Thr Pro His Met
545                 550                 555                 560
Thr His Ser His Trp Ile Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg
565                 570                 575
Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser
580                 585                 590
Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala
595                 600                 605
Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu Asp Arg Met
610                 615                 620
Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly Ser Leu Ile
625                 630                 635                 640
Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe Glu Trp Phe Asp
645                 650                 655
Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu
660                 665                 670
Ala Thr Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg Pro His Ser
675                 680                 685
Asp Asn Gly Phe Gly Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala
690                 695                 700
Asp Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser
705                 710                 715                 720
Glu Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu
725                 730                 735
Asn Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu
740                 745                 750
Thr Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gln
755                 760                 765
Val Glu Asn Ser Val Ile Asn Ala Lys Ile Ala Asp Ala Glu Ala Leu
770                 775                 780
Leu Glu Lys Val Thr Asp Pro Ser Ile Arg Gln Asn Ala Met Glu Thr
785                 790                 795                 800
Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn
805                 810                 815
Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser
820                 825                 830
Gln Pro Ala Pro Ile Gln
835
<210> 9
<211> 2528
<212> DNA
<213> BVH-3
<400> 9
tgtgcctatg cactaaacca gcatcgttcg caggaaaata aggacaataa tcgtgtctct      60
tatgtggatg gcagccagtc aagtcagaaa agtgaaaact tgacaccaga ccaggttagc     120
cagaaagaag gaattcaggc tgagcaaatt gtaatcaaaa ttacagatca gggctatgta     180
acgtcacacg gtgatcacta tcattactat aatgggaaag ttccttatga tgccctcttt     240
agtgaagaac tcttgatgaa ggatccaaac tatcaactta aagacgctga tattgtcaat     300
gaagtcaagg gtggttatat catcaaggtc gatggaaaat attatgtcta cctgaaagat     360
gcagctcatg ctgataatgt tcgaactaaa gatgaaatca atcgtcaaaa acaagaacat     420
gtcaaagata atgagaaggt taactctaat gttgctgtag caaggtctca gggacgatat     480
acgacaaatg atggttatgt ctttaatcca gctgatatta tcgaagatac gggtaatgct     540
tatatcgttc ctcatggagg tcactatcac tacattccca aaagcgattt atctgctagt     600
gaattagcag cagctaaagc acatctggct ggaaaaaata tgcaaccgag tcagttaagc     660
tattcttcaa caccttctcc atctcttcca atcaatccag gaacttcaca tgagaaacat     720
gaagaagatg gatacggatt tgatgctaat cgtattatcg ctgaagatga atcaggtttt     780
gtcatgagtc acggagacca caatcattat ttcttcaaga aggacttgac agaagagcaa     840
attaaggctg cgcaaaaaca tttagaggaa gttaaaacta gtcataatgg attagattct     900
ttgtcatctc atgaacagga ttatccaagt aatgccaaag aaatgaaaga tttagataaa     960
aaaatcgaag aaaaaattgc tggcattatg aaacaatatg gtgtcaaacg tgaaagtatt    1020
gtcgtgaata aagaaaaaaa tgcgattatt tatccgcatg gagatcacca tcatgcagat    1080
ccgattgatg aacataaacc ggttggaatt ggtcattctc acagtaacta tgaactgttt    1140
aaacccgaag aaggagttgc taaaaaagaa gggaataaag tttatactgg agaagaatta    1200
acgaatgttg ttaatttgtt aaaaaatagt acgtttaata atcaaaactt tactctagcc    1260
aatggtcaaa aacgcgtttc ttttagtttt ccgcctgaat tggagaaaaa attaggtatc    1320
aatatgctag taaaattaat aacaccagat ggaaaagtat tggagaaagt atctggtaaa    1380
gtatttggag aaggagtagg gaatattgca aactttgaat tagatcaacc ttatttacca    1440
ggacaaacat ttaagtatac tatcgcttca aaagattatc cagaagtaag ttatgatggt    1500
acatttacag ttccaacctc tttagcttac aaaatggcca gtcaaacgat tttctatcct    1560
ttccatgcag gggatactta tttaagagtg aaccctcaat ttgcagtgcc taaaggaact    1620
gatgctttag tcagagtgtt tgatgaattt catggaaatg cttatttaga aaataactat    1680
aaagttggtg aaatcaaatt accgattccg aaattaaacc aaggaacaac cagaacggcc    1740
ggaaataaaa ttcctgtaac cttcatggca aatgcttatt tggacaatca atcgacttat    1800
attgtggaag tacctatctt ggaaaaagaa aatcaaactg ataaaccaag tattctacca    1860
caatttaaaa ggaataaagc acaagaaaac tcaaaacttg atgaaaaggt agaagaacca    1920
aagactagtg agaaggtaga aaaagaaaaa ctttctgaaa ctgggaatag tactagtaat    1980
tcaacgttag aagaagttcc tacagtggat cctgtacaag aaaaagtagc aaaatttgct    2040
gaaagttatg ggatgaagct agaaaatgtc ttgtttaata tggacggaac aattgaatta    2100
tatttaccat cgggagaagt cattaaaaag aatatggcag attttacagg agaagcacct    2160
caaggaaatg gtgaaaataa accatctgaa aatggaaaag tatctactgg aacagttgag    2220
aaccaaccaa cagaaaataa accagcagat tctttaccag aggcaccaaa cgaaaaacct    2280
gtaaaaccag aaaactcaac ggataatgga atgttgaatc cagaagggaa tgtggggagt    2340
gaccctatgt tagattcagc attagaggaa gctccagcag tagatcctgt acaagaaaaa    2400
ttagaaaaat ttacagctag ttacggatta ggcttagata gtgttatatt caatatggat    2460
ggaacgattg aattaagatt gccaagtgga gaagtgataa aaaagaattt attgatctca    2520
tagcgtaa                                                             2528
<210> 10
<211> 840
<212> PRT
<213> BVH-3
<400> 10
Cys Ala Tyr Ala Leu Asn Gln His Arg Ser Gln Glu Asn Lys Asp Asn
1               5                  10                  15
Asn Arg Val Ser Tyr Val Asp Gly Ser Gln Ser Ser Gln Lys Ser Glu
20                  25                  30
Asn Leu Thr Pro Asp Gln Val Ser Gln Lys Glu Gly Ile Gln Ala Glu
35                  40                  45
Gln Ile Val Ile Lys Ile Thr Asp Gln Gly Tyr Val Thr Ser His Gly
50                  55                  60
Asp His Tyr His Tyr Tyr Asn Gly Lys Val Pro Tyr Asp Ala Leu Phe
65                  70                  75                  80
Ser Glu Glu Leu Leu Met Lys Asp Pro Asn Tyr Gln Leu Lys Asp Ala
85                  90                  95
Asp Ile Val Asn Glu Val Lys Gly Gly Tyr Ile Ile Lys Val Asp Gly
100                 105                 110
Lys Tyr Tyr Val Tyr Leu Lys Asp Ala Ala His Ala Asp Asn Val Arg
115                 120                 125
Thr Lys Asp Glu Ile Asn Arg Gln Lys Gln Glu His Val Lys Asp Asn
130                 135                 140
Glu Lys Val Asn Ser Asn Val Ala Val Ala Arg Ser Gln Gly Arg Tyr
145                 150                 155                 160
Thr Thr Asn Asp Gly Tyr Val Phe Asn Pro Ala Asp Ile Ile Glu Asp
165                 170                 175
Thr Gly Asn Ala Tyr Ile Val Pro His Gly Gly His Tyr His Tyr Ile
180                 185                 190
Pro Lys Ser Asp Leu Ser Ala Ser Glu Leu Ala Ala Ala Lys Ala His
195                 200                 205
Leu Ala Gly Lys Asn Met Gln Pro Ser Gln Leu Ser Tyr Ser Ser Thr
210                 215                 220
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
225                 230                 235                 240
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
245                 250                 255
Glu Ser Gly Phe Val Met Ser His Gly Asp His Asn His Tyr Phe Phe
260                 265                 270
Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu
275                 280                 285
Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His
290                 295                 300
Glu Gln Asp Tyr Pro Ser Asn Ala Lys Glu Met Lys Asp Leu Asp Lys
305                 310                 315                 320
Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys
325                 330                 335
Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro
340                 345                 350
His Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val
355                 360                 365
Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu
370                 375                 380
Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu
385                 390                 395                 400
Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn
405                 410                 415
Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro
420                 425                 430
Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr
435                 440                 445
Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu
450                 455                 460
Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro
465                 470                 475                 480
Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val
485                 490                 495
Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met
500                 505                 510
Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu
515                 520                 525
Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val
530                 535                 540
Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr
545                 550                 555                 560
Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr
565                 570                 575
Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala
580                 585                 590
Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu
595                 600                 605
Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg
610                 615                 620
Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro
625                 630                 635                 640
Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn
645                 650                 655
Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val
660                 665                 670
Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu
675                 680                 685
Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser
690                 695                 700
Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro
705                 710                 715                 720
Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr
725                 730                 735
Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu
740                 745                 750
Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp
755                 760                 765
Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu
770                 775                 780
Asp Ser Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys
785                 790                 795                 800
Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile
805                 810                 815
Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val
820                 825                 830
Ile Lys Lys Asn Leu Leu Ile Ser
835                 840
<210> 11
<211> 94
<212> PRT
<213> Unknown
<220>
<223> 4D4.9
<400> 11
Asp Gln Gly Tyr Val Thr Ser His Gly Asp His Tyr His Tyr Tyr Asn
1               5                  10                  15
Gly Lys Val Pro Tyr Asp Ala Leu Phe Ser Glu Glu Leu Leu Met Lys
20                  25                  30
Asp Pro Asn Tyr Gln Leu Lys Asp Ala Asp Ile Val Asn Glu Val Lys
35                  40                  45
Gly Gly Tyr Ile Ile Lys Val Asp Gly Lys Tyr Tyr Val Tyr Leu Lys
50                  55                  60
Asp Ala Ala His Ala Asp Asn Val Arg Thr Lys Asp Glu Ile Asn Arg
65                  70                  75                  80
Gln Lys Gln Glu His Val Lys Asp Asn Glu Lys Val Asn Ser
85                  90
<210> 12
<211> 42
<212> PRT
<213> Unknown
<220>
<223> 7G11.7
<400> 12
Gly Ile Gln Ala Glu Gln Ile Val Ile Lys Ile Thr Asp Gln Gly Tyr
1               5                  10                  15
Val Thr Ser His Gly Asp His Tyr His Tyr Tyr Asn Gly Lys Val Pro
20                  25                  30
Tyr Asp Ala Leu Phe Ser Glu Glu Leu Leu
35                  40
<210> 13
<211> 39
<212> PRT
<213> Unknown
<220>
<223> 7G11.9
<400> 13
Thr Ala Tyr Ile Val Arg His Gly Asp His Phe His Tyr Ile Pro Lys
1               5                  10                  15
Ser Asn Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
20                  25                  30
Pro Ser Pro Ser Leu Pro Ile
35
<210> 14
<211> 36
<212> PRT
<213> Unknown
<220>
<223> 4D3.4
<400> 14
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
1               5                  10                  15
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
20                  25                  30
Val Leu Phe Asn
35
<210> 15
<211> 24
<212> PRT
<213> Unknown
<220>
<223> 8E3.1
<400> 15
Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys
1               5                  10                  15
Lys Asn Leu Ser Asp Phe Ile Ala
20
<210> 16
<211> 35
<212> PRT
<213> Unknown
<220>
<223> 1G2.2
<400> 16
Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile
1               5                  10                  15
Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp
20                  25                  30
Phe Ile Ala
35
<210> 17
<211> 57
<212> PRT
<213> Unknown
<220>
<223> 10C12.7
<400> 17
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
1               5                  10                  15
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
20                  25                  30
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
35                  40                  45
Lys Lys Asn Leu Ser Asp Phe Ile Ala
50                  55
<210> 18
<211> 40
<212> PRT
<213> Unknown
<220>
<223> 14F6.3
<400> 18
Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu
1               5                  10                  15
Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro
20                  25                  30
Thr Val Asp Pro Val Gln Glu Lys
35                  40
<210> 19
<211> 78
<212> PRT
<213> Unknown
<220>
<223> B12D8.2
<400> 19
Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly Ile Met
1               5                  10                  15
Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys Glu Lys
20                  25                  30
Asn Ala Ile Ile Tyr Pro His Gly Asp His His His Ala Asp Pro Ile
35                  40                  45
Asp Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu
50                  55                  60
Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn
65                  70                  75
<210> 20
<211> 51
<212> PRT
<213> Unknown
<220>
<223> 7F4.1
<400> 20
Ala Ile Ile Tyr Pro His Gly Asp His His His Ala Asp Pro Ile Asp
1               5                  10                  15
Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu
20                  25                  30
Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr
35                  40                  45
Thr Gly Glu
50
<210> 21
<211> 29
<212> PRT
<213> Unknown
<220>
<223> 10D7.5
<400> 21
Ile Gln Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr
1               5                  10                  15
Ile Phe Asp Pro Arg Asp Ile Thr Ser Asp Glu Gly Asp
20                  25
<210> 22
<211> 86
<212> PRT
<213> Unknown
<220>
<223> 10G9.3 and 10A2.2 and B11B8.1
<400> 22
Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile
1               5                  10                  15
Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro
20                  25                  30
Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile
35                  40                  45
Pro His Tyr Asp His Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu
50                  55                  60
Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala
65                  70                  75                  80
Thr Val Lys Tyr Tyr Val
85
<210> 23
<211> 41
<212> PRT
<213> Unknown
<220>
<223> 11B8.4
<400> 23
Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala
1               5                  10                  15
Thr Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg Pro His Ser Asp
20                  25                  30
Asn Gly Phe Gly Asn Ala Ser Asp His
35                  40
<210> 24
<211> 69
<212> PRT
<213> Unknown
<220>
<223> 3A4.1
<400> 24
Val Glu Asn Ser Val Ile Asn Ala Lys Ile Ala Asp Ala Glu Ala Leu
1               5                  10                  15
Leu Glu Lys Val Thr Asp Pro Ser Ile Arg Gln Asn Ala Met Glu Thr
20                  25                  30
Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn
35                  40                  45
Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser
50                  55                  60
Gln Pro Ala Pro Ile
65
<210> 25
<211> 27
<212> DNA
<213> Unknown
<220>
<223> OCRR 479
<400> 25
cagtagatct gtgcctatgc actaaac                                          27
<210> 26
<211> 33
<212> DNA
<213> Unknown
<220>
<223> OCRR 480
<400> 26
gatctctaga ctactgctat tccttacgct atg                                   33
<210> 27
<211> 30
<212> DNA
<213> Unknown
<220>
<223> OCRR 497
<400> 27
atcactcgag cattacctgg ataatcctgt                                       30
<210> 28
<211> 26
<212> DNA
<213> Unknown
<220>
<223> OCRR 498
<400> 28
ctgctaagct tatgaaagat ttagat                                           26
<210> 29
<211> 24
<212> DNA
<213> Unknown
<220>
<223> OCRR 499
<400> 29
gatactcgag ctgctattcc ttac                                             24
<210> 30
<211> 28
<212> DNA
<213> Unknown
<220>
<223> HAMJ 172
<400> 30
gaatctcgag ttaagctgct gctaattc                                         28
<210> 31
<211> 32
<212> DNA
<213> Unknown
<220>
<223> HAMJ 247
<400> 31
gacgctcgag cgctatgaaa tcagataaat tc                                    32
<210> 32
<211> 37
<212> DNA
<213> Unknown
<220>
<223> HAMJ 248
<400> 32
gacgctcgag ggcattacct ggataatcct gttcatg                               37
<210> 33
<211> 33
<212> DNA
<213> Unknown
<220>
<223> HAMJ 249
<400> 33
cagtagatct cttcatcatt tattgaaaag agg                                   33
<210> 34
<211> 40
<212> DNA
<213> Unknown
<220>
<223> HAMJ 278
<400> 34
ttatttcttc catatggact tgacagaaga gcaaattaag                            40
<210> 35
<211> 32
<212> DNA
<213> Unknown
<220>
<223> HAMJ 279
<400> 35
cgccaagctt cgctatgaaa tcagataaat tc                                    32
<210> 36
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 280
<400> 36
cgccaagctt ttccacaata taagtcgatt gatt                                  34
<210> 37
<211> 40
<212> DNA
<213> Unknown
<220>
<223> HAMJ 281
<400> 37
ttatttcttc catatggaag tacctatctt ggaaaaagaa                            40
<210> 38
<211> 37
<212> DNA
<213> Unknown
<220>
<223> HAMJ 300
<400> 38
ttatttcttc catatggtgc ctatgcacta aaccagc                               37
<210> 39
<211> 40
<212> DNA
<213> Unknown
<220>
<223> HAMJ 313
<400> 39
ataagaatgc ggccgcttcc acaatataag tcgattgatt                            40
<210> 40
<211> 31
<212> DNA
<213> Unknown
<220>
<223> OCRR 487
<400> 40
cagtagatct gtgcttatga actaggtttg c                                     31
<210> 41
<211> 32
<212> DNA
<213> Unknown
<220>
<223> OCRR 488
<400> 41
gatcaagctt gctgctacct ttacttactc tc                                    32
<210> 42
<211> 26
<212> DNA
<213> Unknown
<220>
<223> HAMJ 171
<400> 42
ctgagatatc cgttatcgtt caaacc                                           26
<210> 43
<211> 28
<212> DNA
<213> Unknown
<220>
<223> HAMJ 251
<400> 43
ctgcaagctt ttaaagggga ataatacg                                         28
<210> 44
<211> 29
<212> DNA
<213> Unknown
<220>
<223> HAMJ 264
<400> 44
cagtagatct gcagaagcct tcctatctg                                        29
<210> 45
<211> 33
<212> DNA
<213> Unknown
<220>
<223> HAMJ 282
<400> 45
tcgccaagct tcgttatcgt tcaaaccatt ggg                                   33
<210> 46
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 283
<400> 46
ataagaatgc ggccgcctta ctctccttta ataaagccaa tagtt                      45
<210> 47
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 284
<400> 47
catgccatgg acattgatag tctcttgaaa cagc                                  34
<210> 48
<211> 37
<212> DNA
<213> Unknown
<220>
<223> HAMJ 285
<400> 48
cgccaagctt cttactctcc tttaataaag ccaatag                               37
<210> 49
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 286
<400> 49
cgacaagctt aacatggtcg ctagcgttac c                                     31
<210> 50
<211> 30
<212> DNA
<213> Unknown
<220>
<223> HAMJ 287
<400> 50
cataccatgg gcctttatga ggcacctaag                                       30
<210> 51
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 288
<400> 51
cgacaagctt aagtaaatct tcagcctctc tcag                                  34
<210> 52
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 289
<400> 52
gataccatgg ctagcgacca tgttcaaaga a                                     31
<210> 53
<211> 36
<212> DNA
<213> Unknown
<220>
<223> HAMJ 290
<400> 53
cgccaagctt atcatccact aacttgactt tatcac                                36
<210> 54
<211> 33
<212> DNA
<213> Unknown
<220>
<223> HAMJ 291
<400> 54
cataccatgg atattcttgc cttcttagct ccg                                   33
<210> 55
<211> 30
<212> DNA
<213> Unknown
<220>
<223> HAMJ 301
<400> 55
catgccatgg tgcttatgaa ctaggtttgc                                       30
<210> 56
<211> 32
<212> DNA
<213> Unknown
<220>
<223> HAMJ 302
<400> 56
cgccaagctt tagcgttacc aaaaccatta tc                                    32
<210> 57
<211> 36
<212> DNA
<213> Unknown
<220>
<223> HAMJ 160
<400> 57
gtattagatc tgttcctatg aacttggtcg tcacca                                36
<210> 58
<211> 28
<212> DNA
<213> Unknown
<220>
<223> HAMJ 186
<400> 58
cgcctctaga ctactgtata ggagccgg                                         28
<210> 59
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 292
<400> 59
catgccatgg aaaacatttc aagcctttta cgtg                                  34
<210> 60
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 293
<400> 60
cgacaagctt ctgtatagga gccggttgac tttc                                  34
<210> 61
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 294
<400> 61
catgccatgg ttcgtaaaaa taaggcagac caag                                  34
<210> 62
<211> 30
<212> DNA
<213> Unknown
<220>
<223> HAMJ 297
<400> 62
catgccatgg aagcctattg gaatgggaag                                       30
<210> 63
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 352
<400> 63
catgccatgg aagcctattg gaatgggaag c                                     31
<210> 64
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 353
<400> 64
cgccaagctt gtaggtaatt tgcgcatttg g                                     31
<210> 65
<211> 30
<212> DNA
<213> Unknown
<220>
<223> HAMJ 354
<400> 65
cgccaagctt ctgtatagga gccggttgac                                       30
<210> 66
<211> 32
<212> DNA
<213> Unknown
<220>
<223> HAMJ 355
<400> 66
catgccatgg atattcttgc cttcttagct cc                                    32
<210> 67
<211> 38
<212> DNA
<213> Unknown
<220>
<223> HAMJ 404
<400> 67
ttatttcttc catatgcatg gtgatcattt ccattaca                              38
<210> 68
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 464
<400> 68
gatgcatatg aatatgcaac cgagtcagtt aagc                                  34
<210> 69
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 465
<400> 69
gatgctcgag agcatcaaat ccgtatccat c                                     31
<210> 70
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 466
<400> 70
gatgcatatg gatcatttcc attacattcc a                                     31
<210> 71
<211> 30
<212> DNA
<213> Unknown
<220>
<223> HAMJ 467
<400> 71
gacaagcttg gcattacctg gataatcctg                                       30
<210> 72
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 352
<400> 72
catgccatgg aagcctattg gaatgggaag c                                       30
<210> 73
<211> 38
<212> DNA
<213> Unknown
<220>
<223> HAMJ 470
<400> 73
ataagaatgc ggccgccgct atgaaatcag ataaattc                              38
<210> 74
<400> 74
000
<210> 75
<400> 75
000
<210> 76
<400> 76
000
<210> 77
<400> 77
000
<210> 78
<400> 78
000
<210> 79
<400> 79
000
<210> 80
<400> 80
000
<210> 81
<400> 81
000
<210> 82
<400> 82
000
<210> 83
<400> 83
000
<210> 84
<400> 84
000
<210> 85
<400> 85
000
<210> 86
<400> 86
000
<210> 87
<400> 87
000
<210> 88
<400> 88
000
<210> 89
<400> 89
000
<210> 90
<400> 90
000
<210> 91
<400> 91
000
<210> 92
<400> 92
000
<210> 93
<400> 93
000
<210> 94
<400> 94
000
<210> 95
<400> 95
000
<210> 96
<400> 96
000
<210> 97
<400> 97
000
<210> 98
<400> 98
000
<210> 99
<400> 99
000
<210> 100
<400> 100
000
<210> 101
<400> 101
000
<210> 102
<400> 102
000
<210> 103
<400> 103
000
<210> 104
<400> 104
000
<210> 105
<400> 105
000
<210> 106
<400> 106
000
<210> 107
<400> 107
000
<210> 108
<400> 108
000
<210> 109
<400> 109
000
<210> 110
<400> 110
000
<210> 111
<400> 111
000
<210> 112
<400> 112
000
<210> 113
<400> 113
000
<210> 114
<400> 114
000
<210> 115
<400> 115
000
<210> 116
<400> 116
000
<210> 117
<400> 117
000
<210> 118
<400> 118
000
<210> 119
<400> 119
000
<210> 120
<400> 120
000
<210> 121
<400> 121
000
<210> 122
<400> 122
000
<210> 123
<400> 123
000
<210> 124
<400> 124
000
<210> 125
<400> 125
000
<210> 126
<400> 126
000
<210> 127
<400> 127
000
<210> 128
<400> 128
000
<210> 129
<400> 129
000
<210> 130
<400> 130
000
<210> 131
<400> 131
000
<210> 132
<400> 132
000
<210> 133
<400> 133
000
<210> 134
<400> 134
000
<210> 135
<400> 135
000
<210> 136
<400> 136
000
<210> 137
<400> 137
000
<210> 138
<400> 138
000
<210> 139
<400> 139
000
<210> 140
<400> 140
000
<210> 141
<400> 141
000
<210> 142
<400> 142
000
<210> 143
<400> 143
000
<210> 144
<400> 144
000
<210> 145
<400> 145
000
<210> 146
<400> 146
000
<210> 147
<400> 147
000
<210> 148
<400> 148
000
<210> 149
<400> 149
000
<210> 150
<400> 150
000
<210> 151
<400> 151
000
<210> 152
<400> 152
000
<210> 153
<400> 153
000
<210> 154
<400> 154
000
<210> 155
<400> 155
000
<210> 156
<400> 156
000
<210> 157
<400> 157
000
<210> 158
<400> 158
000
<210> 159
<400> 159
000
<210> 160
<400> 160
000
<210> 161
<400> 161
000
<210> 162
<400> 162
000
<210> 163
<211> 22
<212> PRT
<213> Unknown
<220>
<223> Mab H11-B-11B8 target epitope
<400> 163
Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala
1               5                  10                  15
Thr Val Lys Tyr Tyr Val
20
<210> 164
<400> 164
000
<210> 165
<400> 165
000
<210> 166
<400> 166
000
<210> 167
<400> 167
000
<210> 168
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 471
<400> 168
atatgggccc ctgtatagga gccggttgac tttc                                  34
<210> 169
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 472
<400> 169
atatgggccc aatatgcaac cgagtcagtt aagc                                  34
<210> 170
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 350
<400> 170
atatgggccc aacatggtcg ctagcgttac c                                     31
<210> 171
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 351
<400> 171
tcccgggccc gacttgacag aagagcaaat taag                                  34
<210> 172
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 358
<400> 172
catgccatgg gacttgacag aagagcaaat taag                                  34
<210> 173
<211> 32
<212> DNA
<213> Unknown
<220>
<223> HAMJ 359
<400> 173
tcccgggccc cgctatgaaa tcagataaat tc                                    32
<210> 174
<211> 35
<212> DNA
<213> Unknown
<220>
<223> HAMJ 403
<400> 174
atatgggccc gacattgata gtctcttgaa acagc                                 35
<210> 175
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 361
<400> 175
cgccaagctt aacatggtcg ctagcgttac c                                     31
<210> 176
<211> 37
<212> DNA
<213> Unknown
<220>
<223> HAMJ 483
<400> 176
atatgggccc cttactctcc tttaataaag ccaatag                               37
<210> 177
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 513
<400> 177
gaatcaggtt ttgtcatgag ttccggagac cacaatcatt atttc                      45
<210> 178
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 514
<400> 178
gaaataatga ttgtggtctc cggaactcat gacaaaacct gattc                      45
<210> 179
<211> 43
<212> DNA
<213> Unknown
<220>
<223> HAMJ 515
<400> 179
gtcatgagtt ccggagactc caatcattat ttcttcaaga agg                        43
<210> 180
<211> 43
<212> DNA
<213> Unknown
<220>
<223> HAMJ 516
<400> 180
ccttcttgaa gaaataatga ttggagtctc cggaactcat gac                        43
<210> 181
<211> 44
<212> DNA
<213> Unknown
<220>
<223> HAMJ 517
<400> 181
atgagttcgg agactccaat tcttatttct tcaagaagga cttg                       44
<210> 182
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 518
<400> 182
caagtccttc ttgaagaaat aagaattgga gtctccggaa ctcat                      45
<210> 183
<211> 35
<212> DNA
<213> Unknown
<220>
<223> CHAN 51
<400> 183
gcgattattt atccgtctgg agatcaccat catgc                                 35
<210> 184
<211> 35
<212> DNA
<213> Unknown
<220>
<223> CHAN 52
<400> 184
gcatgatggt gatctccaga cggataaata atcgc                                 35
<210> 185
<211> 30
<212> DNA
<213> Unknown
<220>
<223> CHAN 53
<400> 185
ccgtctggag atggccatca tgcagatccg                                       30
<210> 186
<211> 30
<212> DNA
<213> Unknown
<220>
<223> CHAN 54
<400> 186
cggatctgca tgatggccat ctccagacgg                                       30
<210> 187
<211> 34
<212> DNA
<213> Unknown
<220>
<223> CHAN 47
<400> 187
ccgcagggag ataagcgtca tgcagatccg attg                                  34
<210> 188
<211> 34
<212> DNA
<213> Unknown
<220>
<223> CHAN 48
<400> 188
caatcggatc tgcatgacgc ttatctccct gcgg                                  34
<210> 189
<211> 34
<212> DNA
<213> Unknown
<220>
<223> CHAN 55
<400> 189
ccgtctggag atggcactca tgcagatccg attg                                  34
<210> 190
<211> 34
<212> DNA
<213> Unknown
<220>
<223> CHAN 56
<400> 190
caatcggatc tgcatgagtg ccatctccag acgg                                  34
<210> 191
<211> 37
<212> DNA
<213> Unknown
<220>
<223> CHAN 57
<400> 191
ccgtctggag atggcacttc tgcagatccg attgatg                               37
<210> 192
<211> 37
<212> DNA
<213> Unknown
<220>
<223> CHAN 58
<400> 192
catcaatcgg atctgcagaa gtgccatctc cagacgg                               37
<210> 193
<211> 30
<212> DNA
<213> Unknown
<220>
<223> HAMJ 523
<400> 193
ccgcatggag atggccatca tgcagatccg                                       30
<210> 194
<211> 30
<212> DNA
<213> Unknown
<220>
<223> HAMJ 524
<400> 194
cggatctgca tgatggccat ctccatgcgg                                       30
<210> 195
<211> 43
<212> DNA
<213> Unknown
<220>
<223> HAMJ 526
<400> 195
gtcatgagtc acggagactc caatcattat ttcttcaaga agg                        43
<210> 196
<211> 43
<212> DNA
<213> Unknown
<220>
<223> HAMJ 527
<400> 196
ccttcttgaa gaaataatga ttggagtctc cgtgactcat gac                        43
<210> 197
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 528
<400> 197
atgagtcacg gagaccacaa ttcttatttc ttcaagaagg acttg                      45
<210> 198
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 529
<400> 198
caagtccttc ttgaagaaat aagaattgtg gtctccgtga ctcat                      45
<210> 199
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 569
<400> 199
tacctcatta tgactcttac tctaacatca aatttgagtg gtttg                      45
<210> 200
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 570
<400> 200
caaaccactc aaatttgatg ttagagtaag agtcataatg aggta                      45
<210> 201
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 571
<400> 201
taccttctta tgaccattac tctaacatca aatttgagtg gtttg                      45
<210> 202
<211> 44
<212> DNA
<213> Unknown
<220>
<223> HAMJ 572
<400> 202
aaaccactca aatttgatgt tagagtaatg gtcataagaa ggta                       44
<210> 203
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 573
<400> 203
aacggtagtt taatcatacc ttctaaagac cattaccata acatc                      45
<210> 204
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 574
<400> 204
gatgttatgg taatggtctt tagaaggtat gattaaacta ccgtt                      45
<210> 205
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 575
<400> 205
cggtagttta atcatacctc ataaggactc ttaccataac atcaaa                     46
<210> 206
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 576
<400> 206
tttgatgtta tggtaagagt ccttatgagg tatgattaaa ctaccg                     46
<210> 207
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 577
<400> 207
aacggtagtt taatcatacc tgaccattac cataacatca aatttg                     46
<210> 208
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 578
<400> 208
caaatttgat gttatggtaa tggtcaggta tgattaaact accgtt                     46
<210> 209
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 579
<400> 209
aacggtagtt taatcatacc ttaccataac atcaaatttg agtgg                      45
<210> 210
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 580
<400> 210
ccactcaaat ttgatgttat ggtaaggtat gattaaacta ccgtt                      45
<210> 211
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 581
<400> 211
accggtagtt taatcatacc taacatcaaa tttgagtggt ttgac                      45
<210> 212
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 582
<400> 212
gtcaaaccac tcaaatttga tgttaggtat gattaaacta ccgtt                      45
<210> 213
<211> 35
<212> DNA
<213> Unknown
<220>
<223> HAMJ 536
<400> 213
cctatgtaac tccacatata acccatagcc actgg                                 35
<210> 214
<211> 35
<212> DNA
<213> Unknown
<220>
<223> HAMJ 537
<400> 214
ccagtggcta tgggttatat gtggagttac atagg                                 35
<210> 215
<211> 36
<212> DNA
<213> Unknown
<220>
<223> HAMJ 550
<400> 215
cgtgaaagta ttgtcgtaaa taaagaaaaa aatgcg                                36
<210> 216
<211> 36
<212> DNA
<213> Unknown
<220>
<223> HAMJ 551
<400> 216
cgcatttttt tctttattta cgacaatact ttcacg                                36
<210> 217
<211> 49
<212> DNA
<213> Unknown
<220>
<223> HAMJ 586
<400> 217
catgaagaag atggttacgg tttcgatgct aaccgtatta tcgctgaag                  49
<210> 218
<211> 48
<212> DNA
<213> Unknown
<220>
<223> HAMJ 587
<400> 218
cttcagcgat aatacggtta gcatcgaaac cgtaaccatc ttcttctg                   48
<210> 219
<211> 42
<212> DNA
<213> Unknown
<220>
<223> HAMJ 588
<400> 219
gaatcaggtt ttgtcatgag tgaccacaat cattatttct tc                         42
<210> 220
<211> 42
<212> DNA
<213> Unknown
<220>
<223> HAMJ 589
<400> 220
gaagaaataa tgattgtggt cactcatgac aaaacctgat tc                         42
<210> 221
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 590
<400> 221
gaagatgaat caggttttgt catgagtaat cattatttct tcaag                      45
<210> 222
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 591
<400> 222
cttgaagaaa taatgattac tcatgacaaa acctgattca tcttc                      45
<210> 223
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 592
<400> 223
gaagatgaat caggttttgt catgagttat ttcttcaaga aggac                      45
<210> 224
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 593
<400> 224
gtccttcttg aagaaataac tcatgacaaa acctgattca tcttc                      45
<210> 225
<211> 42
<212> DNA
<213> Unknown
<220>
<223> HAMJ 594
<400> 225
aaaatgcgat tatttatccg caccatcatg cagatccgat tg                         42
<210> 226
<211> 42
<212> DNA
<213> Unknown
<220>
<223> HAMJ 595
<400> 226
caatcggatc tgcatgatgg tgcggataaa taatcgcatt tt                         42
<210> 227
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 600
<400> 227
aaaatgcgat tatttatccg gcagatccga ttgatgaaca taaac                      45
<210> 228
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 601
<400> 228
gtttatgttc atcaatcgga tctgccggat aaataatcgc atttt                      45
<210> 229
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 604
<400> 229
gatgctaacc gtataatcgc cgaagacgaa tcaggttttg tcatg                      45
<210> 230
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 605
<400> 230
catgacaaaa cctgattcgt cttcggcgat tatacggtta gcatc                      45
<210> 231
<211> 43
<212> DNA
<213> Unknown
<220>
<223> HAMJ 606
<400> 231
cgccgaagac gaatccggct ttgtaatgag tcacggagac tcc                        43
<210> 232
<211> 43
<212> DNA
<213> Unknown
<220>
<223> HAMJ 607
<400> 232
ggagtctccg tgactcatta caaagccgga ttcgtcttcg gcg                        43
<210> 233
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 608
<400> 233
catctcatga acaggattat cccggcaacg ccaaagaaat gaaag                      45
<210> 234
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 609
<400> 234
ctttcatttc tttggcgttg ccgggataat cctgttcatg agatg                      45
<210> 235
<211> 569
<212> PRT
<213> Unknown
<220>
<223> NEW 1
<400> 235
Met Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
1               5                  10                  15
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
20                  25                  30
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
35                  40                  45
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
50                  55                  60
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
65                  70                  75                  80
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
85                  90                  95
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
100                 105                 110
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
115                 120                 125
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
130                 135                 140
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
145                 150                 155                 160
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
165                 170                 175
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
180                 185                 190
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
195                 200                 205
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
210                 215                 220
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
225                 230                 235                 240
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
245                 250                 255
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
260                 265                 270
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
275                 280                 285
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
290                 295                 300
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
305                 310                 315                 320
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
325                 330                 335
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
340                 345                 350
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
355                 360                 365
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
370                 375                 380
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
385                 390                 395                 400
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
405                 410                 415
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
420                 425                 430
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
435                 440                 445
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
450                 455                 460
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
465                 470                 475                 480
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
485                 490                 495
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
500                 505                 510
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
515                 520                 525
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
530                 535                 540
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
545                 550                 555                 560
Lys Lys Asn Leu Ser Asp Phe Ile Ala
565
<210> 236
<400> 236
000
<210> 237
<400> 237
000
<210> 238
<400> 238
000
<210> 239
<400> 239
000
<210> 240
<400> 240
000
<210> 241
<400> 241
000
<210> 242
<400> 242
000
<210> 243
<400> 243
000
<210> 244
<400> 244
000
<210> 245
<400> 245
000
<210> 246
<400> 246
000
<210> 247
<400> 247
000
<210> 248
<400> 248
000
<210> 249
<400> 249
000
<210> 250
<400> 250
000
<210> 251
<400> 251
000
<210> 252
<400> 252
000
<210> 253
<400> 253
000
<210> 254
<400> 254
000
<210> 255
<211> 569
<212> PRT
<213> Unknown
<220>
<223> NEW 1
<400> 255
Met Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
1               5                  10                  15
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
20                  25                  30
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
35                  40                  45
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
50                  55                  60
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
65                  70                  75                  80
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
85                  90                  95
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
100                 105                 110
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
115                 120                 125
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
130                 135                 140
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
145                 150                 155                 160
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
165                 170                 175
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
180                 185                 190
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
195                 200                 205
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
210                 215                 220
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
225                 230                 235                 240
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
245                 250                 255
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
260                 265                 270
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
275                 280                 285
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
290                 295                 300
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
305                 310                 315                 320
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
325                 330                 335
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
340                 345                 350
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
355                 360                 365
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
370                 375                 380
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
385                 390                 395                 400
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
405                 410                 415
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
420                 425                 430
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
435                 440                 445
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
450                 455                 460
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
465                 470                 475                 480
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
485                 490                 495
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
500                 505                 510
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
515                 520                 525
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
530                 535                 540
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
545                 550                 555                 560
Lys Lys Asn Leu Ser Asp Phe Ile Ala
565
<210> 256
<211> 569
<212> PRT
<213> Unknown
<220>
<223> NEW 35A
<400> 256
Met Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
1               5                  10                  15
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
20                  25                  30
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
35                  40                  45
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
50                  55                  60
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
65                  70                  75                  80
Gly Asp Gly Thr Ser Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
85                  90                  95
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
100                 105                 110
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
115                 120                 125
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
130                 135                 140
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
145                 150                 155                 160
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
165                 170                 175
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
180                 185                 190
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
195                 200                 205
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
210                 215                 220
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
225                 230                 235                 240
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
245                 250                 255
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
260                 265                 270
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
275                 280                 285
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
290                 295                 300
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
305                 310                 315                 320
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
325                 330                 335
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
340                 345                 350
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
355                 360                 365
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
370                 375                 380
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
385                 390                 395                 400
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
405                 410                 415
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
420                 425                 430
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
435                 440                 445
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
450                 455                 460
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
465                 470                 475                 480
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
485                 490                 495
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
500                 505                 510
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
515                 520                 525
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
530                 535                 540
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
545                 550                 555                 560
Lys Lys Asn Leu Ser Asp Phe Ile Ala
565
<210> 257
<211> 819
<212> DNA
<213> Unknown
<220>
<223> NEW43 gene
<400> 257
atgcaaatta cctacactga tgatgagatt caggtagcca agttggcagg caagtacaca      60
acagaagacg gttatatctt tgatactagt tggattaaaa aagatagttt gtctgaagct     120
gagagagcgg cagcccaggc ttatgctaaa gagaaaggtt tgacccctcc ttcgacagac     180
caccaggatt caggaaatac tgaggcaaaa ggagcagaag ctatctacaa ccgcgtgaaa     240
gcagctaaga aggtgccact tgatcgtatg ccttacaatc ttcagtatac tgtagaagtc     300
aaaaacggta gtttaatcat acctcattat gaccattacc ataacatcaa atttgagtgg     360
tttgacgaag gcctttatga ggcacctaag gggtatagtc ttgaggatct tttggcgact     420
gtcaagtact atgtcgaacc gcggaacgct agtgaccatg ttcgtaaaaa taaggcagac     480
caagatagta aacctgatga agataaggaa catgatgaag taagtgagcc aactcaccct     540
gaatctgatg aaaaagagaa tcacgctggt ttaaatcctt cagcagataa tctttataaa     600
ccaagcactg atacggaaga gacagaggaa gaagctgaag ataccacaga tgaggctgaa     660
attcctggta cccctagtat tagacaaaat gctatggaga cattgactgg tctaaaaagt     720
agtcttcttc tcggaacgaa agataataac actatttcag cagaagtaga tagtctcttg     780
gctttgttaa aagaaagtca accggctcct atacagtag                            819
<210> 258
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW43 polypeptide
<400> 258
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 259
<211> 36
<212> DNA
<213> Unknown
<220>
<223> HAMJ 490
<400> 259
ccgaattcca tatgcaaatt acctacactg atgatg                                36
<210> 260
<211> 29
<212> DNA
<213> Unknown
<220>
<223> HAMJ 491
<400> 260
ggactagtat caaagatata accgtcttc                                        29
<210> 261
<211> 33
<212> DNA
<213> Unknown
<220>
<223> HAMJ 492
<400> 261
ggactagttg gattaaaaaa gatagtttgt ctg                                   33
<210> 262
<211> 32
<212> DNA
<213> Unknown
<220>
<223> HAMJ 493
<400> 262
ttcccgcggt tcgacatagt acttgacagt cg                                    32
<210> 263
<211> 29
<212> DNA
<213> Unknown
<220>
<223> HAMJ 494
<400> 263
ttcccgcgga acgctagtga ccatgttcg                                        29
<210> 264
<211> 31
<212> DNA
<213> Unknown
<220>
<223> HAMJ 495
<400> 264
cggggtacca ggaatttcag cctcatctgt g                                     31
<210> 265
<211> 36
<212> DNA
<213> Unknown
<220>
<223> HAMJ 496
<400> 265
cccggtaccc ctagtattag acaaaatgct atggag                                36
<210> 266
<211> 29
<212> DNA
<213> Unknown
<220>
<223> HAMJ 583
<400> 266
ggatcccggg aggtatgatt aaactaccg                                        29
<210> 267
<211> 34
<212> DNA
<213> Unknown
<220>
<223> HAMJ 584
<400> 267
catgcccggg aacatcaaat ttgagtggtt tgac                                  34
<210> 268
<211> 37
<212> DNA
<213> Unknown
<220>
<223> HAMJ 610
<400> 268
cttgatcgac atatgttggc aggcaagtac acaacag                               37
<210> 269
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 497
<400> 269
aacggtagtt taatcatacc ttcttatgac cattaccata acatc                      45
<210> 270
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 498
<400> 270
gatgttatgg taatggtcat aagaaggtat gattaaacta ccgtt                      45
<210> 271
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 499
<400> 271
aatcatacct tcttatgact cttaccataa catcaaattt gagtg                      45
<210> 272
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 500
<400> 272
cactcaaatt tgatgttatg gtaagagtca taagaaggta tgatt                      45
<210> 273
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 501
<400> 273
taccttctta tgactcttac tctaacatca aatttgagtg gtttg                      45
<210> 274
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 502
<400> 274
caaaccactc aaatttgatg ttagagtaag agtcataaga aggta                      45
<210> 275
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 530
<400> 275
aatcatacct cattatgact cttaccataa catcaaattt gagtg                      45
<210> 276
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 531
<400> 276
cactcaaatt tgatgttatg gtaagagtca taatgaggta tgatt                      45
<210> 277
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 532
<400> 277
tacctcatta tgaccattac tctaacatca aatttgagtg gtttg                      45
<210> 278
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 533
<400> 278
caaaccactc aaatttgatg ttagagtaat ggtcataatg aggta                      45
<210> 279
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 569
<400> 279
tacctcatta tgactcttac tctaacatca aatttgagtg gtttg                      45
<210> 280
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 570
<400> 280
caaaccactc aaatttgatg ttagagtaag agtcataatg aggta                      45
<210> 281
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 571
<400> 281
taccttctta tgaccattac tctaacatca aatttgagtg gtttg                      45
<210> 282
<211> 44
<212> DNA
<213> Unknown
<220>
<223> HAMJ 572
<400> 282
aaaccactca aatttgatgt tagagtaatg gtcataagaa ggta                       44
<210> 283
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 573
<400> 283
aacggtagtt taatcatacc ttctaaagac cattaccata acatc                      45
<210> 284
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 574
<400> 284
gatgttatgg taatggtctt tagaaggtat gattaaacta ccgtt                      45
<210> 285
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 575
<400> 285
cggtagttta atcatacctc ataaggactc ttaccataac atcaaa                     46
<210> 286
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 576
<400> 286
tttgatgtta tggtaagagt ccttatgagg tatgattaaa ctaccg                     46
<210> 287
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 577
<400> 287
aacggtagtt taatcatacc tgaccattac cataacatca aatttg                     46
<210> 288
<211> 46
<212> DNA
<213> Unknown
<220>
<223> HAMJ 578
<400> 288
caaatttgat gttatggtaa tggtcaggta tgattaaact accgtt                     46
<210> 289
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 579
<400> 289
aacggtagtt taatcatacc ttaccataac atcaaatttg agtgg                      45
<210> 290
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 580
<400> 290
ccactcaaat ttgatgttat ggtaaggtat gattaaacta ccgtt                      45
<210> 291
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 581
<400> 291
accggtagtt taatcatacc taacatcaaa tttgagtggt ttgac                      45
<210> 292
<211> 45
<212> DNA
<213> Unknown
<220>
<223> HAMJ 582
<400> 292
gtcaaaccac tcaaatttga tgttaggtat gattaaacta ccgtt                      45
<210> 293
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 60
<400> 293
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 294
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 61
<400> 294
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp Ser
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 295
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 62
<400> 295
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp His
100                 105                 110
Tyr Ser Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 296
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 80
<400> 296
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp Ser
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 297
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 81
<400> 297
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp Ser
100                 105                 110
Tyr Ser Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 298
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 82
<400> 298
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp Ser
100                 105                 110
Tyr Ser Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 299
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 83
<400> 299
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp His
100                 105                 110
Tyr Ser Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 300
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 84
<400> 300
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Lys Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 301
<211> 272
<212> PRT
<213> Unknown
<220>
<223> NEW 85
<400> 301
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Lys Asp Ser
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 302
<211> 270
<212> PRT
<213> Unknown
<220>
<223> NEW 88D1
<400> 302
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Asp His Tyr His
100                 105                 110
Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys
115                 120                 125
Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu
130                 135                 140
Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp
145                 150                 155                 160
Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr
165                 170                 175
His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser
180                 185                 190
Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu
195                 200                 205
Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser
210                 215                 220
Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu
225                 230                 235                 240
Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser
245                 250                 255
Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
<210> 303
<211> 268
<212> PRT
<213> Unknown
<220>
<223> NEW 88D2
<400> 303
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Tyr His Asn Ile
100                 105                 110
Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr
115                 120                 125
Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg
130                 135                 140
Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys
145                 150                 155                 160
Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro
165                 170                 175
Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp
180                 185                 190
Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala
195                 200                 205
Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg
210                 215                 220
Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu
225                 230                 235                 240
Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu
245                 250                 255
Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265
<210> 304
<211> 266
<212> PRT
<213> Unknown
<220>
<223> NEW 88
<400> 304
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Asn Ile Lys Phe
100                 105                 110
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
115                 120                 125
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
130                 135                 140
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
145                 150                 155                 160
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
165                 170                 175
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
180                 185                 190
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
195                 200                 205
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
210                 215                 220
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
225                 230                 235                 240
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
245                 250                 255
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265
<210> 305
<400> 305
000
<210> 306
<400> 306
000
<210> 307
<400> 307
000
<210> 308
<400> 308
000
<210> 309
<400> 309
000
<210> 310
<400> 310
000
<210> 311
<400> 311
000
<210> 312
<400> 312
000
<210> 313
<400> 313
000
<210> 314
<400> 314
000
<210> 315
<400> 315
000
<210> 316
<400> 316
000
<210> 317
<400> 317
000
<210> 318
<400> 318
000
<210> 319
<400> 319
000
<210> 320
<400> 320
000
<210> 321
<400> 321
000
<210> 322
<400> 322
000
<210> 323
<400> 323
000
<210> 324
<400> 324
000
<210> 325
<400> 325
000
<210> 326
<400> 326
000
<210> 327
<211> 41
<212> DNA
<213> Unknown
<220>
<223> HAMJ 564
<400> 327
atatgggccc caaattacct acactgatga tgagattcag g                          41
<210> 328
<211> 43
<212> DNA
<213> Unknown
<220>
<223> HAMJ 556
<400> 328
ataagaatgc ggccgcctac tgtataggag ccggttgact ttc                        43
<210> 329
<211> 33
<212> DNA
<213> Unknown
<220>
<223> HAMJ 489
<400> 329
ccgaattcca tatgcaaatt gggcaaccga ctc                                   33
<210> 330
<211> 29
<212> DNA
<213> Unknown
<220>
<223> HAMJ 559
<400> 330
atatgggccc caaattgggc aaccgactc                                        29
<210> 331
<211> 41
<212> DNA
<213> Unknown
<220>
<223> HAMJ 557
<400> 331
ataagaatgc ggccgcttac gctatgaaat cagataaatt c                          41
<210> 332
<211> 906
<212> PRT
<213> Unknown
<220>
<223> VP 94
<400> 332
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe
325                 330                 335
Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu
340                 345                 350
Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His
355                 360                 365
Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys
370                 375                 380
Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys
385                 390                 395                 400
Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro
405                 410                 415
Ser Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val
420                 425                 430
Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu
435                 440                 445
Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu
450                 455                 460
Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn
465                 470                 475                 480
Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro
485                 490                 495
Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr
500                 505                 510
Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu
515                 520                 525
Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro
530                 535                 540
Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val
545                 550                 555                 560
Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met
565                 570                 575
Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu
580                 585                 590
Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val
595                 600                 605
Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr
610                 615                 620
Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr
625                 630                 635                 640
Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala
645                 650                 655
Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu
660                 665                 670
Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg
675                 680                 685
Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro
690                 695                 700
Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn
705                 710                 715                 720
Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val
725                 730                 735
Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu
740                 745                 750
Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser
755                 760                 765
Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro
770                 775                 780
Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr
785                 790                 795                 800
Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu
805                 810                 815
Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp
820                 825                 830
Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu
835                 840                 845
Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys
850                 855                 860
Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile
865                 870                 875                 880
Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val
885                 890                 895
Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
900                 905
<210> 333
<211> 900
<212> PRT
<213> Unknown
<220>
<223> VP 108
<400> 333
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala Gly Pro Gln Ile Thr Tyr Thr
625                 630                 635                 640
Asp Asp Glu Ile Gln Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu
645                 650                 655
Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile Lys Lys Asp Ser Leu Ser
660                 665                 670
Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu
675                 680                 685
Thr Pro Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys
690                 695                 700
Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro
705                 710                 715                 720
Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn
725                 730                 735
Gly Ser Leu Ile Ile Pro Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly
740                 745                 750
Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr
755                 760                 765
Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys
770                 775                 780
Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp
785                 790                 795                 800
Glu Val Ser Glu Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His
805                 810                 815
Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp
820                 825                 830
Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu
835                 840                 845
Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr
850                 855                 860
Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile
865                 870                 875                 880
Ser Ala Glu Val Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro
885                 890                 895
Ala Pro Ile Gln
900
<210> 334
<211> 900
<212> PRT
<213> Unknown
<220>
<223> VP 109
<400> 334
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Asn Ile Lys Phe
100                 105                 110
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
115                 120                 125
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
130                 135                 140
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
145                 150                 155                 160
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
165                 170                 175
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
180                 185                 190
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
195                 200                 205
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
210                 215                 220
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
225                 230                 235                 240
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
245                 250                 255
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln Gly Pro Gln Ile Gly Gln
260                 265                 270
Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu Pro
275                 280                 285
Ile Asn Pro Gly Thr Ser His Glu Lys His Glu Glu Asp Gly Tyr Gly
290                 295                 300
Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe Val Met
305                 310                 315                 320
Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys Lys Asp Leu Thr Glu
325                 330                 335
Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser
340                 345                 350
His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly
355                 360                 365
Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile
370                 375                 380
Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val
385                 390                 395                 400
Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser Gly Asp His His His
405                 410                 415
Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His
420                 425                 430
Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu
435                 440                 445
Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu
450                 455                 460
Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly
465                 470                 475                 480
Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu
485                 490                 495
Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu
500                 505                 510
Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala
515                 520                 525
Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr
530                 535                 540
Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe
545                 550                 555                 560
Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe
565                 570                 575
Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe
580                 585                 590
Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe
595                 600                 605
His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys
610                 615                 620
Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn
625                 630                 635                 640
Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser
645                 650                 655
Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp
660                 665                 670
Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn
675                 680                 685
Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val
690                 695                 700
Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr
705                 710                 715                 720
Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys
725                 730                 735
Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met
740                 745                 750
Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys
755                 760                 765
Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn
770                 775                 780
Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln
785                 790                 795                 800
Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu
805                 810                 815
Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro
820                 825                 830
Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu
835                 840                 845
Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala
850                 855                 860
Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr
865                 870                 875                 880
Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser
885                 890                 895
Asp Phe Ile Ala
900
<210> 335
<211> 900
<212> PRT
<213> Unknown
<220>
<223> VP 110
<400> 335
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu
325                 330                 335
Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser
340                 345                 350
His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly
355                 360                 365
Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile
370                 375                 380
Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val
385                 390                 395                 400
Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His His
405                 410                 415
Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His
420                 425                 430
Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu
435                 440                 445
Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu
450                 455                 460
Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly
465                 470                 475                 480
Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu
485                 490                 495
Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu
500                 505                 510
Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala
515                 520                 525
Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr
530                 535                 540
Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe
545                 550                 555                 560
Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe
565                 570                 575
Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe
580                 585                 590
Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe
595                 600                 605
His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys
610                 615                 620
Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn
625                 630                 635                 640
Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser
645                 650                 655
Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp
660                 665                 670
Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn
675                 680                 685
Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val
690                 695                 700
Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr
705                 710                 715                 720
Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys
725                 730                 735
Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met
740                 745                 750
Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys
755                 760                 765
Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn
770                 775                 780
Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln
785                 790                 795                 800
Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu
805                 810                 815
Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro
820                 825                 830
Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu
835                 840                 845
Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala
850                 855                 860
Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr
865                 870                 875                 880
Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser
885                 890                 895
Asp Phe Ile Ala
900
<210> 336
<211> 894
<212> PRT
<213> Unknown
<220>
<223> VP 111
<400> 336
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu
325                 330                 335
Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser
340                 345                 350
His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly
355                 360                 365
Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile
370                 375                 380
Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val
385                 390                 395                 400
Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ala Asp Pro Ile Asp Glu
405                 410                 415
His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe
420                 425                 430
Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr
435                 440                 445
Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe
450                 455                 460
Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe
465                 470                 475                 480
Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val
485                 490                 495
Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys
500                 505                 510
Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln
515                 520                 525
Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp
530                 535                 540
Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu
545                 550                 555                 560
Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly
565                 570                 575
Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr
580                 585                 590
Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu
595                 600                 605
Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu
610                 615                 620
Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe
625                 630                 635                 640
Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val
645                 650                 655
Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro
660                 665                 670
Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys
675                 680                 685
Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser
690                 695                 700
Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr
705                 710                 715                 720
Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly
725                 730                 735
Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu
740                 745                 750
Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr
755                 760                 765
Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly
770                 775                 780
Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro
785                 790                 795                 800
Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu
805                 810                 815
Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser
820                 825                 830
Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro
835                 840                 845
Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu
850                 855                 860
Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro
865                 870                 875                 880
Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
885                 890
<210> 337
<211> 894
<212> PRT
<213> Unknown
<220>
<223> VP 112
<400> 337
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Asn Ile Lys Phe
100                 105                 110
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
115                 120                 125
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
130                 135                 140
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
145                 150                 155                 160
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
165                 170                 175
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
180                 185                 190
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
195                 200                 205
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
210                 215                 220
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
225                 230                 235                 240
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
245                 250                 255
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln Gly Pro Gln Ile Gly Gln
260                 265                 270
Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu Pro
275                 280                 285
Ile Asn Pro Gly Thr Ser His Glu Lys His Glu Glu Asp Gly Tyr Gly
290                 295                 300
Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe Val Met
305                 310                 315                 320
Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala
325                 330                 335
Gln Lys His Leu Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser
340                 345                 350
Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys
355                 360                 365
Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln
370                 375                 380
Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala
385                 390                 395                 400
Ile Ile Tyr Pro His Gly Asp His His His Ala Asp Pro Ile Asp Glu
405                 410                 415
His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe
420                 425                 430
Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr
435                 440                 445
Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe
450                 455                 460
Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe
465                 470                 475                 480
Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val
485                 490                 495
Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys
500                 505                 510
Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln
515                 520                 525
Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp
530                 535                 540
Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu
545                 550                 555                 560
Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly
565                 570                 575
Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr
580                 585                 590
Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu
595                 600                 605
Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu
610                 615                 620
Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe
625                 630                 635                 640
Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val
645                 650                 655
Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro
660                 665                 670
Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys
675                 680                 685
Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser
690                 695                 700
Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr
705                 710                 715                 720
Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly
725                 730                 735
Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu
740                 745                 750
Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr
755                 760                 765
Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly
770                 775                 780
Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro
785                 790                 795                 800
Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu
805                 810                 815
Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser
820                 825                 830
Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro
835                 840                 845
Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu
850                 855                 860
Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro
865                 870                 875                 880
Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
885                 890
<210> 338
<211> 888
<212> PRT
<213> Unknown
<220>
<223> VP 113
<400> 338
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Asn Ile Lys Phe
100                 105                 110
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
115                 120                 125
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
130                 135                 140
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
145                 150                 155                 160
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
165                 170                 175
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
180                 185                 190
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
195                 200                 205
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
210                 215                 220
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
225                 230                 235                 240
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
245                 250                 255
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln Gly Pro Gln Ile Gly Gln
260                 265                 270
Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu Pro
275                 280                 285
Ile Asn Pro Gly Thr Ser His Glu Lys His Glu Glu Asp Gly Tyr Gly
290                 295                 300
Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe Val Met
305                 310                 315                 320
Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala
325                 330                 335
Gln Lys His Leu Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser
340                 345                 350
Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys
355                 360                 365
Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln
370                 375                 380
Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala
385                 390                 395                 400
Ile Ile Tyr Pro Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile
405                 410                 415
Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val
420                 425                 430
Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn
435                 440                 445
Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr
450                 455                 460
Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu
465                 470                 475                 480
Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp
485                 490                 495
Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val
500                 505                 510
Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln
515                 520                 525
Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr
530                 535                 540
Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser
545                 550                 555                 560
Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val
565                 570                 575
Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val
580                 585                 590
Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val
595                 600                 605
Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg
610                 615                 620
Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu
625                 630                 635                 640
Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu
645                 650                 655
Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys
660                 665                 670
Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr
675                 680                 685
Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr
690                 695                 700
Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu
705                 710                 715                 720
Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val
725                 730                 735
Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu
740                 745                 750
Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly
755                 760                 765
Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr
770                 775                 780
Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu
785                 790                 795                 800
Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly
805                 810                 815
Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro
820                 825                 830
Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu
835                 840                 845
Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn
850                 855                 860
Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys
865                 870                 875                 880
Lys Asn Leu Ser Asp Phe Ile Ala
885
<210> 339
<211> 900
<212> PRT
<213> Unknown
<220>
<223> VP 114
<400> 339
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp Ser
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu
325                 330                 335
Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser
340                 345                 350
His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly
355                 360                 365
Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile
370                 375                 380
Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val
385                 390                 395                 400
Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His His
405                 410                 415
Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His
420                 425                 430
Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu
435                 440                 445
Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu
450                 455                 460
Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly
465                 470                 475                 480
Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu
485                 490                 495
Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu
500                 505                 510
Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala
515                 520                 525
Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr
530                 535                 540
Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe
545                 550                 555                 560
Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe
565                 570                 575
Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe
580                 585                 590
Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe
595                 600                 605
His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys
610                 615                 620
Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn
625                 630                 635                 640
Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser
645                 650                 655
Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp
660                 665                 670
Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn
675                 680                 685
Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val
690                 695                 700
Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr
705                 710                 715                 720
Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys
725                 730                 735
Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met
740                 745                 750
Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys
755                 760                 765
Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn
770                 775                 780
Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln
785                 790                 795                 800
Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu
805                 810                 815
Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro
820                 825                 830
Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu
835                 840                 845
Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala
850                 855                 860
Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr
865                 870                 875                 880
Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser
885                 890                 895
Asp Phe Ile Ala
900
<210> 340
<211> 894
<212> PRT
<213> Unknown
<220>
<223> VP 115
<400> 340
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp Ser
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu
325                 330                 335
Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser
340                 345                 350
His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly
355                 360                 365
Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile
370                 375                 380
Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val
385                 390                 395                 400
Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ala Asp Pro Ile Asp Glu
405                 410                 415
His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe
420                 425                 430
Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr
435                 440                 445
Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe
450                 455                 460
Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe
465                 470                 475                 480
Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val
485                 490                 495
Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys
500                 505                 510
Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln
515                 520                 525
Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp
530                 535                 540
Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu
545                 550                 555                 560
Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly
565                 570                 575
Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr
580                 585                 590
Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu
595                 600                 605
Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu
610                 615                 620
Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe
625                 630                 635                 640
Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val
645                 650                 655
Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro
660                 665                 670
Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys
675                 680                 685
Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser
690                 695                 700
Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr
705                 710                 715                 720
Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly
725                 730                 735
Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu
740                 745                 750
Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr
755                 760                 765
Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly
770                 775                 780
Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro
785                 790                 795                 800
Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu
805                 810                 815
Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser
820                 825                 830
Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro
835                 840                 845
Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu
850                 855                 860
Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro
865                 870                 875                 880
Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
885                 890
<210> 341
<211> 900
<212> PRT
<213> Unknown
<220>
<223> VP 116
<400> 341
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp His
100                 105                 110
Tyr Ser Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu
325                 330                 335
Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser
340                 345                 350
His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly
355                 360                 365
Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile
370                 375                 380
Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val
385                 390                 395                 400
Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His His
405                 410                 415
Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His
420                 425                 430
Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu
435                 440                 445
Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu
450                 455                 460
Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly
465                 470                 475                 480
Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu
485                 490                 495
Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu
500                 505                 510
Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala
515                 520                 525
Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr
530                 535                 540
Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe
545                 550                 555                 560
Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe
565                 570                 575
Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe
580                 585                 590
Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe
595                 600                 605
His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys
610                 615                 620
Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn
625                 630                 635                 640
Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser
645                 650                 655
Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp
660                 665                 670
Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn
675                 680                 685
Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val
690                 695                 700
Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr
705                 710                 715                 720
Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys
725                 730                 735
Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met
740                 745                 750
Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys
755                 760                 765
Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn
770                 775                 780
Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln
785                 790                 795                 800
Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu
805                 810                 815
Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro
820                 825                 830
Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu
835                 840                 845
Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala
850                 855                 860
Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr
865                 870                 875                 880
Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser
885                 890                 895
Asp Phe Ile Ala
900
<210> 342
<211> 894
<212> PRT
<213> Unknown
<220>
<223> VP 117
<400> 342
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp His
100                 105                 110
Tyr Ser Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu
325                 330                 335
Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser
340                 345                 350
His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly
355                 360                 365
Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile
370                 375                 380
Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val
385                 390                 395                 400
Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ala Asp Pro Ile Asp Glu
405                 410                 415
His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe
420                 425                 430
Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr
435                 440                 445
Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe
450                 455                 460
Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe
465                 470                 475                 480
Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val
485                 490                 495
Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys
500                 505                 510
Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln
515                 520                 525
Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp
530                 535                 540
Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu
545                 550                 555                 560
Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly
565                 570                 575
Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr
580                 585                 590
Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu
595                 600                 605
Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu
610                 615                 620
Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe
625                 630                 635                 640
Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val
645                 650                 655
Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro
660                 665                 670
Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys
675                 680                 685
Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser
690                 695                 700
Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr
705                 710                 715                 720
Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly
725                 730                 735
Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu
740                 745                 750
Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr
755                 760                 765
Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly
770                 775                 780
Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro
785                 790                 795                 800
Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu
805                 810                 815
Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser
820                 825                 830
Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro
835                 840                 845
Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu
850                 855                 860
Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro
865                 870                 875                 880
Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
885                 890
<210> 343
<211> 901
<212> PRT
<213> Unknown
<220>
<223> VP 119
<400> 343
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Ser Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala
275                 280                 285
Thr Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys
290                 295                 300
His Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu
305                 310                 315                 320
Asp Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr
325                 330                 335
Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr
340                 345                 350
Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro
355                 360                 365
Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys
370                 375                 380
Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val
385                 390                 395                 400
Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His
405                 410                 415
His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser
420                 425                 430
His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys
435                 440                 445
Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn
450                 455                 460
Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn
465                 470                 475                 480
Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys
485                 490                 495
Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val
500                 505                 510
Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile
515                 520                 525
Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys
530                 535                 540
Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr
545                 550                 555                 560
Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile
565                 570                 575
Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln
580                 585                 590
Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu
595                 600                 605
Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile
610                 615                 620
Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly
625                 630                 635                 640
Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln
645                 650                 655
Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr
660                 665                 670
Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu
675                 680                 685
Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys
690                 695                 700
Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser
705                 710                 715                 720
Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala
725                 730                 735
Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn
740                 745                 750
Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys
755                 760                 765
Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu
770                 775                 780
Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn
785                 790                 795                 800
Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn
805                 810                 815
Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn
820                 825                 830
Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu
835                 840                 845
Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr
850                 855                 860
Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly
865                 870                 875                 880
Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu
885                 890                 895
Ser Asp Phe Ile Ala
900
<210> 344
<211> 895
<212> PRT
<213> Unknown
<220>
<223> VP 120
<400> 344
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Ser Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala
275                 280                 285
Thr Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys
290                 295                 300
His Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu
305                 310                 315                 320
Asp Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr
325                 330                 335
Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr
340                 345                 350
Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro
355                 360                 365
Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys
370                 375                 380
Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val
385                 390                 395                 400
Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ala Asp Pro Ile Asp
405                 410                 415
Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu
420                 425                 430
Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr
435                 440                 445
Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr
450                 455                 460
Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser
465                 470                 475                 480
Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu
485                 490                 495
Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly
500                 505                 510
Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp
515                 520                 525
Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys
530                 535                 540
Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser
545                 550                 555                 560
Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala
565                 570                 575
Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly
580                 585                 590
Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr
595                 600                 605
Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys
610                 615                 620
Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr
625                 630                 635                 640
Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu
645                 650                 655
Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu
660                 665                 670
Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu
675                 680                 685
Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu
690                 695                 700
Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro
705                 710                 715                 720
Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr
725                 730                 735
Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu
740                 745                 750
Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe
755                 760                 765
Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn
770                 775                 780
Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys
785                 790                 795                 800
Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro
805                 810                 815
Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly
820                 825                 830
Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp
835                 840                 845
Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly
850                 855                 860
Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu
865                 870                 875                 880
Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
885                 890                 895
<210> 345
<211> 901
<212> PRT
<213> Unknown
<220>
<223> VP 121
<400> 345
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp Ser
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Ser Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala
275                 280                 285
Thr Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys
290                 295                 300
His Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu
305                 310                 315                 320
Asp Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr
325                 330                 335
Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr
340                 345                 350
Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro
355                 360                 365
Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys
370                 375                 380
Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val
385                 390                 395                 400
Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His
405                 410                 415
His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser
420                 425                 430
His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys
435                 440                 445
Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn
450                 455                 460
Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn
465                 470                 475                 480
Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys
485                 490                 495
Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val
500                 505                 510
Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile
515                 520                 525
Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys
530                 535                 540
Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr
545                 550                 555                 560
Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile
565                 570                 575
Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln
580                 585                 590
Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu
595                 600                 605
Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile
610                 615                 620
Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly
625                 630                 635                 640
Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln
645                 650                 655
Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr
660                 665                 670
Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu
675                 680                 685
Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys
690                 695                 700
Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser
705                 710                 715                 720
Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala
725                 730                 735
Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn
740                 745                 750
Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys
755                 760                 765
Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu
770                 775                 780
Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn
785                 790                 795                 800
Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn
805                 810                 815
Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn
820                 825                 830
Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu
835                 840                 845
Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr
850                 855                 860
Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly
865                 870                 875                 880
Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu
885                 890                 895
Ser Asp Phe Ile Ala
900
<210> 346
<211> 895
<212> PRT
<213> Unknown
<220>
<223> VP 122
<400> 346
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Ser Tyr Asp Ser
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Ser Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala
275                 280                 285
Thr Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys
290                 295                 300
His Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu
305                 310                 315                 320
Asp Glu Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr
325                 330                 335
Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr
340                 345                 350
Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro
355                 360                 365
Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys
370                 375                 380
Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val
385                 390                 395                 400
Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ala Asp Pro Ile Asp
405                 410                 415
Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu
420                 425                 430
Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr
435                 440                 445
Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr
450                 455                 460
Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser
465                 470                 475                 480
Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu
485                 490                 495
Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly
500                 505                 510
Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp
515                 520                 525
Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys
530                 535                 540
Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser
545                 550                 555                 560
Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala
565                 570                 575
Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly
580                 585                 590
Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr
595                 600                 605
Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys
610                 615                 620
Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr
625                 630                 635                 640
Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu
645                 650                 655
Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu
660                 665                 670
Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu
675                 680                 685
Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu
690                 695                 700
Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro
705                 710                 715                 720
Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr
725                 730                 735
Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu
740                 745                 750
Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe
755                 760                 765
Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn
770                 775                 780
Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys
785                 790                 795                 800
Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro
805                 810                 815
Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly
820                 825                 830
Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp
835                 840                 845
Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly
850                 855                 860
Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu
865                 870                 875                 880
Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
885                 890                 895
<210> 347
<211> 895
<212> PRT
<213> Unknown
<220>
<223> VP 123
<400> 347
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Asn Ile Lys Phe
100                 105                 110
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
115                 120                 125
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
130                 135                 140
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
145                 150                 155                 160
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
165                 170                 175
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
180                 185                 190
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
195                 200                 205
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
210                 215                 220
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
225                 230                 235                 240
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
245                 250                 255
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln Ser Gly Pro Gln Ile Gly
260                 265                 270
Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu
275                 280                 285
Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu Glu Asp Gly Tyr
290                 295                 300
Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe Val
305                 310                 315                 320
Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala
325                 330                 335
Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp
340                 345                 350
Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met
355                 360                 365
Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys
370                 375                 380
Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn
385                 390                 395                 400
Ala Ile Ile Tyr Pro His Gly Asp His His His Ala Asp Pro Ile Asp
405                 410                 415
Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu
420                 425                 430
Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr
435                 440                 445
Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr
450                 455                 460
Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser
465                 470                 475                 480
Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu
485                 490                 495
Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly
500                 505                 510
Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp
515                 520                 525
Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys
530                 535                 540
Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser
545                 550                 555                 560
Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala
565                 570                 575
Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly
580                 585                 590
Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr
595                 600                 605
Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys
610                 615                 620
Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr
625                 630                 635                 640
Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu
645                 650                 655
Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu
660                 665                 670
Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu
675                 680                 685
Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu
690                 695                 700
Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro
705                 710                 715                 720
Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr
725                 730                 735
Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu
740                 745                 750
Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe
755                 760                 765
Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn
770                 775                 780
Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys
785                 790                 795                 800
Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro
805                 810                 815
Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly
820                 825                 830
Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp
835                 840                 845
Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly
850                 855                 860
Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu
865                 870                 875                 880
Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
885                 890                 895
<210> 348
<211> 889
<212> PRT
<213> Unknown
<220>
<223> VP 124
<400> 348
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Asn Ile Lys Phe
100                 105                 110
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
115                 120                 125
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
130                 135                 140
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
145                 150                 155                 160
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
165                 170                 175
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
180                 185                 190
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
195                 200                 205
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
210                 215                 220
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
225                 230                 235                 240
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
245                 250                 255
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln Ser Gly Pro Gln Ile Gly
260                 265                 270
Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu
275                 280                 285
Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu Glu Asp Gly Tyr
290                 295                 300
Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe Val
305                 310                 315                 320
Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala
325                 330                 335
Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp
340                 345                 350
Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met
355                 360                 365
Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys
370                 375                 380
Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn
385                 390                 395                 400
Ala Ile Ile Tyr Pro Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
405                 410                 415
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
420                 425                 430
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
435                 440                 445
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
450                 455                 460
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
465                 470                 475                 480
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
485                 490                 495
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
500                 505                 510
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
515                 520                 525
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
530                 535                 540
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
545                 550                 555                 560
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
565                 570                 575
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
580                 585                 590
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
595                 600                 605
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
610                 615                 620
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
625                 630                 635                 640
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
645                 650                 655
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
660                 665                 670
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
675                 680                 685
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
690                 695                 700
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
705                 710                 715                 720
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
725                 730                 735
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
740                 745                 750
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
755                 760                 765
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
770                 775                 780
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
785                 790                 795                 800
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
805                 810                 815
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
820                 825                 830
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
835                 840                 845
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
850                 855                 860
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
865                 870                 875                 880
Lys Lys Asn Leu Ser Asp Phe Ile Ala
885
<210> 349
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 42
<400> 349
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Ser Gly Asp Ser Asn Ser Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp Gly Thr Ser Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 350
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 49
<400> 350
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Ser Gly Asp His Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 351
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 50
<400> 351
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Ser Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 352
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 51
<400> 352
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Ser Gly Asp His Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 353
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 52
<400> 353
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Ser Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 354
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 53
<400> 354
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp His Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 355
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 54
<400> 355
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp His Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 356
<211> 569
<212> PRT
<213> Unknown
<220>
<223> NEW 55
<400> 356
Met Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
1               5                  10                  15
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
20                  25                  30
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
35                  40                  45
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
50                  55                  60
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
65                  70                  75                  80
Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
85                  90                  95
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
100                 105                 110
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
115                 120                 125
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
130                 135                 140
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
145                 150                 155                 160
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
165                 170                 175
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
180                 185                 190
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
195                 200                 205
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
210                 215                 220
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
225                 230                 235                 240
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
245                 250                 255
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
260                 265                 270
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
275                 280                 285
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
290                 295                 300
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
305                 310                 315                 320
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
325                 330                 335
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
340                 345                 350
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
355                 360                 365
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
370                 375                 380
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
385                 390                 395                 400
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
405                 410                 415
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
420                 425                 430
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
435                 440                 445
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
450                 455                 460
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
465                 470                 475                 480
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
485                 490                 495
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
500                 505                 510
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
515                 520                 525
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
530                 535                 540
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
545                 550                 555                 560
Lys Lys Asn Leu Ser Asp Phe Ile Ala
565
<210> 357
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 56
<400> 357
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 358
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 56
<400> 358
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 359
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 56
<400> 359
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 360
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 57
<400> 360
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp His Asn Ser Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 361
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 63
<400> 361
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 362
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 64
<400> 362
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp His Asn Ser Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 363
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 65
<400> 363
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
130                 135                 140
Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 364
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 66
<400> 364
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp His Asn Ser Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
130                 135                 140
Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 365
<211> 633
<212> PRT
<213> Unknown
<220>
<223> NEW 76
<400> 365
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp His Asn Ser Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala
625                 630
<210> 366
<211> 627
<212> PRT
<213> Unknown
<220>
<223> NEW 105
<400> 366
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu
50                  55                  60
Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His
65                  70                  75                  80
Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn
85                  90                  95
Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala
100                 105                 110
Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn
115                 120                 125
Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His His Ala
130                 135                 140
Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His Ser
145                 150                 155                 160
Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly
165                 170                 175
Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu
180                 185                 190
Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln
195                 200                 205
Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly
210                 215                 220
Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu
225                 230                 235                 240
Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn
245                 250                 255
Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr
260                 265                 270
Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr
275                 280                 285
Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr
290                 295                 300
Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala
305                 310                 315                 320
Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His
325                 330                 335
Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu
340                 345                 350
Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys
355                 360                 365
Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr
370                 375                 380
Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys
385                 390                 395                 400
Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser
405                 410                 415
Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu
420                 425                 430
Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu
435                 440                 445
Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe
450                 455                 460
Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp
465                 470                 475                 480
Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn
485                 490                 495
Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys
500                 505                 510
Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro
515                 520                 525
Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys
530                 535                 540
Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu
545                 550                 555                 560
Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala
565                 570                 575
Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser
580                 585                 590
Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile
595                 600                 605
Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp
610                 615                 620
Phe Ile Ala
625
<210> 367
<211> 627
<212> PRT
<213> Unknown
<220>
<223> NEW 106
<400> 367
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp His Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ala
130                 135                 140
Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His Ser
145                 150                 155                 160
Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly
165                 170                 175
Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu
180                 185                 190
Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln
195                 200                 205
Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly
210                 215                 220
Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu
225                 230                 235                 240
Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn
245                 250                 255
Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr
260                 265                 270
Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr
275                 280                 285
Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr
290                 295                 300
Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala
305                 310                 315                 320
Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His
325                 330                 335
Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu
340                 345                 350
Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys
355                 360                 365
Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr
370                 375                 380
Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys
385                 390                 395                 400
Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser
405                 410                 415
Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu
420                 425                 430
Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu
435                 440                 445
Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe
450                 455                 460
Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp
465                 470                 475                 480
Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn
485                 490                 495
Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys
500                 505                 510
Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro
515                 520                 525
Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys
530                 535                 540
Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu
545                 550                 555                 560
Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala
565                 570                 575
Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser
580                 585                 590
Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile
595                 600                 605
Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp
610                 615                 620
Phe Ile Ala
625
<210> 368
<211> 621
<212> PRT
<213> Unknown
<220>
<223> NEW 107
<400> 368
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu
50                  55                  60
Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His
65                  70                  75                  80
Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn
85                  90                  95
Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala
100                 105                 110
Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn
115                 120                 125
Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ala Asp Pro Ile Asp Glu His
130                 135                 140
Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys
145                 150                 155                 160
Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly
165                 170                 175
Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn
180                 185                 190
Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser
195                 200                 205
Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys
210                 215                 220
Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val
225                 230                 235                 240
Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro
245                 250                 255
Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr
260                 265                 270
Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala
275                 280                 285
Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp
290                 295                 300
Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp
305                 310                 315                 320
Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu
325                 330                 335
Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn
340                 345                 350
Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met
355                 360                 365
Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro
370                 375                 380
Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln
385                 390                 395                 400
Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val
405                 410                 415
Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu
420                 425                 430
Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val
435                 440                 445
Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met
450                 455                 460
Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr
465                 470                 475                 480
Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly
485                 490                 495
Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys
500                 505                 510
Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala
515                 520                 525
Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn
530                 535                 540
Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp
545                 550                 555                 560
Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val
565                 570                 575
Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp
580                 585                 590
Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser
595                 600                 605
Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
610                 615                 620
<210> 369
<211> 906
<212> PRT
<213> Unknown
<220>
<223> VP 89
<400> 369
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala Gly Pro Gln Ile Thr Tyr Thr
625                 630                 635                 640
Asp Asp Glu Ile Gln Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu
645                 650                 655
Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile Lys Lys Asp Ser Leu Ser
660                 665                 670
Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu
675                 680                 685
Thr Pro Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys
690                 695                 700
Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro
705                 710                 715                 720
Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn
725                 730                 735
Gly Ser Leu Ile Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe
740                 745                 750
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
755                 760                 765
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
770                 775                 780
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
785                 790                 795                 800
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
805                 810                 815
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
820                 825                 830
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
835                 840                 845
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
850                 855                 860
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
865                 870                 875                 880
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
885                 890                 895
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
900                 905
<210> 370
<211> 906
<212> PRT
<213> Unknown
<220>
<223> VP 90
<400> 370
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe
325                 330                 335
Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu
340                 345                 350
Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His
355                 360                 365
Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys
370                 375                 380
Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys
385                 390                 395                 400
Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro
405                 410                 415
Ser Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val
420                 425                 430
Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu
435                 440                 445
Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu
450                 455                 460
Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn
465                 470                 475                 480
Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro
485                 490                 495
Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr
500                 505                 510
Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu
515                 520                 525
Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro
530                 535                 540
Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val
545                 550                 555                 560
Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met
565                 570                 575
Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu
580                 585                 590
Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val
595                 600                 605
Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr
610                 615                 620
Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr
625                 630                 635                 640
Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala
645                 650                 655
Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu
660                 665                 670
Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg
675                 680                 685
Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro
690                 695                 700
Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn
705                 710                 715                 720
Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val
725                 730                 735
Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu
740                 745                 750
Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser
755                 760                 765
Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro
770                 775                 780
Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr
785                 790                 795                 800
Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu
805                 810                 815
Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp
820                 825                 830
Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu
835                 840                 845
Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys
850                 855                 860
Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile
865                 870                 875                 880
Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val
885                 890                 895
Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
900                 905
<210> 371
<211> 906
<212> PRT
<213> Unknown
<220>
<223> VP 91
<400> 371
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser Ser Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala Gly Pro Gln Ile Thr Tyr Thr
625                 630                 635                 640
Asp Asp Glu Ile Gln Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu
645                 650                 655
Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile Lys Lys Asp Ser Leu Ser
660                 665                 670
Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu
675                 680                 685
Thr Pro Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys
690                 695                 700
Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro
705                 710                 715                 720
Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn
725                 730                 735
Gly Ser Leu Ile Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe
740                 745                 750
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
755                 760                 765
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
770                 775                 780
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
785                 790                 795                 800
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
805                 810                 815
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
820                 825                 830
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
835                 840                 845
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
850                 855                 860
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
865                 870                 875                 880
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
885                 890                 895
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
900                 905
<210> 372
<211> 906
<212> PRT
<213> Unknown
<220>
<223> VP 92
<400> 372
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr Asp His
100                 105                 110
Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala
115                 120                 125
Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr
130                 135                 140
Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp
145                 150                 155                 160
Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu
165                 170                 175
Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn
180                 185                 190
Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr
195                 200                 205
Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr
210                 215                 220
Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser
225                 230                 235                 240
Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val
245                 250                 255
Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265                 270
Gly Pro Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr
275                 280                 285
Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His
290                 295                 300
Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp
305                 310                 315                 320
Glu Ser Gly Phe Val Met Ser Ser Gly Asp Ser Asn His Tyr Phe Phe
325                 330                 335
Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu
340                 345                 350
Glu Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His
355                 360                 365
Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys
370                 375                 380
Lys Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys
385                 390                 395                 400
Arg Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro
405                 410                 415
Ser Gly Asp Gly His His Ala Asp Pro Ile Asp Glu His Lys Pro Val
420                 425                 430
Gly Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu
435                 440                 445
Gly Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu
450                 455                 460
Thr Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn
465                 470                 475                 480
Phe Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro
485                 490                 495
Glu Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr
500                 505                 510
Pro Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu
515                 520                 525
Gly Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro
530                 535                 540
Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val
545                 550                 555                 560
Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met
565                 570                 575
Ala Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu
580                 585                 590
Arg Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val
595                 600                 605
Arg Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr
610                 615                 620
Lys Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr
625                 630                 635                 640
Thr Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala
645                 650                 655
Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu
660                 665                 670
Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg
675                 680                 685
Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro
690                 695                 700
Lys Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn
705                 710                 715                 720
Ser Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val
725                 730                 735
Gln Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu
740                 745                 750
Asn Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser
755                 760                 765
Gly Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro
770                 775                 780
Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr
785                 790                 795                 800
Gly Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu
805                 810                 815
Pro Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp
820                 825                 830
Asn Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu
835                 840                 845
Asp Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys
850                 855                 860
Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile
865                 870                 875                 880
Phe Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val
885                 890                 895
Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
900                 905
<210> 373
<211> 906
<212> PRT
<213> Unknown
<220>
<223> VP 93
<400> 373
Met Gln Ile Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro
1               5                  10                  15
Ser Pro Ser Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu
20                  25                  30
Glu Asp Gly Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu
35                  40                  45
Ser Gly Phe Val Met Ser His Gly Asp Ser Asn His Tyr Phe Phe Lys
50                  55                  60
Lys Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
65                  70                  75                  80
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
85                  90                  95
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
100                 105                 110
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
115                 120                 125
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro Ser
130                 135                 140
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
145                 150                 155                 160
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
165                 170                 175
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
180                 185                 190
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
195                 200                 205
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
210                 215                 220
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
225                 230                 235                 240
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
245                 250                 255
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
260                 265                 270
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
275                 280                 285
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
290                 295                 300
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
305                 310                 315                 320
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
325                 330                 335
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
340                 345                 350
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
355                 360                 365
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
370                 375                 380
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
385                 390                 395                 400
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
405                 410                 415
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
420                 425                 430
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
435                 440                 445
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
450                 455                 460
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
465                 470                 475                 480
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
485                 490                 495
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
500                 505                 510
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
515                 520                 525
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
530                 535                 540
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
545                 550                 555                 560
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
565                 570                 575
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
580                 585                 590
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
595                 600                 605
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
610                 615                 620
Lys Lys Asn Leu Ser Asp Phe Ile Ala Gly Pro Gln Ile Thr Tyr Thr
625                 630                 635                 640
Asp Asp Glu Ile Gln Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu
645                 650                 655
Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile Lys Lys Asp Ser Leu Ser
660                 665                 670
Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu
675                 680                 685
Thr Pro Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys
690                 695                 700
Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro
705                 710                 715                 720
Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn
725                 730                 735
Gly Ser Leu Ile Ile Pro Ser Tyr Asp His Tyr His Asn Ile Lys Phe
740                 745                 750
Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu
755                 760                 765
Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg Asn Ala
770                 775                 780
Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp
785                 790                 795                 800
Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro Glu Ser
805                 810                 815
Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu
820                 825                 830
Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp
835                 840                 845
Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg Gln Asn
850                 855                 860
Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr
865                 870                 875                 880
Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu
885                 890                 895
Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
900                 905
<210> 374
<211> 259
<212> PRT
<213> Unknown
<220>
<223> VP43S
<400> 374
Met Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr
1               5                  10                  15
Ser Trp Ile Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala
20                  25                  30
Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His
35                  40                  45
Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn
50                  55                  60
Arg Val Lys Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn
65                  70                  75                  80
Leu Gln Tyr Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His
85                  90                  95
Tyr Asp His Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu
100                 105                 110
Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu Asp Leu Leu Ala Thr Val
115                 120                 125
Lys Tyr Tyr Val Glu Pro Arg Asn Ala Ser Asp His Val Arg Lys Asn
130                 135                 140
Lys Ala Asp Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu
145                 150                 155                 160
Val Ser Glu Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala
165                 170                 175
Gly Leu Asn Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr
180                 185                 190
Glu Glu Thr Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile
195                 200                 205
Pro Gly Thr Pro Ser Ile Arg Gln Asn Ala Met Glu Thr Leu Thr Gly
210                 215                 220
Leu Lys Ser Ser Leu Leu Leu Gly Thr Lys Asp Asn Asn Thr Ile Ser
225                 230                 235                 240
Ala Glu Val Asp Ser Leu Leu Ala Leu Leu Lys Glu Ser Gln Pro Ala
245                 250                 255
Pro Ile Gln
<210> 375
<211> 268
<212> PRT
<213> Unknown
<220>
<223> NEW 86
<400> 375
Met Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala
1               5                  10                  15
Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Thr Ser Trp Ile
20                  25                  30
Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln Ala Tyr
35                  40                  45
Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln Asp Ser
50                  55                  60
Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg Val Lys
65                  70                  75                  80
Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu Gln Tyr
85                  90                  95
Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro Pro Gly Asn Ile
100                 105                 110
Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr
115                 120                 125
Ser Leu Glu Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu Pro Arg
130                 135                 140
Asn Ala Ser Asp His Val Arg Lys Asn Lys Ala Asp Gln Asp Ser Lys
145                 150                 155                 160
Pro Asp Glu Asp Lys Glu His Asp Glu Val Ser Glu Pro Thr His Pro
165                 170                 175
Glu Ser Asp Glu Lys Glu Asn His Ala Gly Leu Asn Pro Ser Ala Asp
180                 185                 190
Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu Glu Thr Glu Glu Glu Ala
195                 200                 205
Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro Gly Thr Pro Ser Ile Arg
210                 215                 220
Gln Asn Ala Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu
225                 230                 235                 240
Gly Thr Lys Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu
245                 250                 255
Ala Leu Leu Lys Glu Ser Gln Pro Ala Pro Ile Gln
260                 265
<210> 376
<211> 999
<212> PRT
<213> Unknown
<220>
<223> NEW 17
<221> VARIANT
<222> (1)...(1)
<223> Xaa = Methionine or nothing
<221> VARIANT
<222> (430)...(430)
<223> Xaa = Glycine or nothing
<221> VARIANT
<222> (431)...(431)
<223> Xaa = Proline or nothing
<400> 376
Xaa Asp Ile Asp Ser Leu Leu Lys Gln Leu Tyr Lys Leu Pro Leu Ser
1               5                  10                  15
Gln Arg His Val Glu Ser Asp Gly Leu Ile Phe Asp Pro Ala Gln Ile
20                  25                  30
Thr Ser Arg Thr Ala Arg Gly Val Ala Val Pro His Gly Asn His Tyr
35                  40                  45
His Phe Ile Pro Tyr Glu Gln Met Ser Glu Leu Glu Lys Arg Ile Ala
50                  55                  60
Arg Ile Ile Pro Leu Arg Tyr Arg Ser Asn His Trp Val Pro Asp Ser
65                  70                  75                  80
Arg Pro Glu Glu Pro Ser Pro Gln Pro Thr Pro Glu Pro Ser Pro Ser
85                  90                  95
Pro Gln Pro Ala Pro Asn Pro Gln Pro Ala Pro Ser Asn Pro Ile Asp
100                 105                 110
Glu Lys Leu Val Lys Glu Ala Val Arg Lys Val Gly Asp Gly Tyr Val
115                 120                 125
Phe Glu Glu Asn Gly Val Ser Arg Tyr Ile Pro Ala Lys Asn Leu Ser
130                 135                 140
Ala Glu Thr Ala Ala Gly Ile Asp Ser Lys Leu Ala Lys Gln Glu Ser
145                 150                 155                 160
Leu Ser His Lys Leu Gly Ala Lys Lys Thr Asp Leu Pro Ser Ser Asp
165                 170                 175
Arg Glu Phe Tyr Asn Lys Ala Tyr Asp Leu Leu Ala Arg Ile His Gln
180                 185                 190
Asp Leu Leu Asp Asn Lys Gly Arg Gln Val Asp Phe Glu Ala Leu Asp
195                 200                 205
Asn Leu Leu Glu Arg Leu Lys Asp Val Ser Ser Asp Lys Val Lys Leu
210                 215                 220
Val Asp Asp Ile Leu Ala Phe Leu Ala Pro Ile Arg His Pro Glu Arg
225                 230                 235                 240
Leu Gly Lys Pro Asn Ala Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln
245                 250                 255
Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe
260                 265                 270
Asp Pro Arg Asp Ile Thr Ser Asp Glu Gly Asp Ala Tyr Val Thr Pro
275                 280                 285
His Met Thr His Ser His Trp Ile Lys Lys Asp Ser Leu Ser Glu Ala
290                 295                 300
Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr Pro
305                 310                 315                 320
Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala
325                 330                 335
Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu Asp
340                 345                 350
Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly Ser
355                 360                 365
Leu Ile Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe Glu Trp
370                 375                 380
Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Thr Leu Glu Asp
385                 390                 395                 400
Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg Pro
405                 410                 415
His Ser Asp Asn Gly Phe Gly Asn Ala Ser Asp His Val Xaa Xaa Asp
420                 425                 430
Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val
435                 440                 445
Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp
450                 455                 460
Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu
465                 470                 475                 480
Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser
485                 490                 495
Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp
500                 505                 510
His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly
515                 520                 525
His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala
530                 535                 540
Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val
545                 550                 555                 560
Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu
565                 570                 575
Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu
580                 585                 590
Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly
595                 600                 605
Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly
610                 615                 620
Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr
625                 630                 635                 640
Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp
645                 650                 655
Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln
660                 665                 670
Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn
675                 680                 685
Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe
690                 695                 700
Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly
705                 710                 715                 720
Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr
725                 730                 735
Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp
740                 745                 750
Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn
755                 760                 765
Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala
770                 775                 780
Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser
785                 790                 795                 800
Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser
805                 810                 815
Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys
820                 825                 830
Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu
835                 840                 845
Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val
850                 855                 860
Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn
865                 870                 875                 880
Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val
885                 890                 895
Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala
900                 905                 910
Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met
915                 920                 925
Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala
930                 935                 940
Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys
945                 950                 955                 960
Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met
965                 970                 975
Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys
980                 985                 990
Asn Leu Ser Asp Phe Ile Ala
995
<210> 377
<211> 999
<212> PRT
<213> Unknown
<220>
<223> NEW 20
<221> VARIANT
<222> (1)...(1)
<223> Xaa = Methionine or nothing
<221> VARIANT
<222> (570)...(570)
<223> Xaa = Glycine or nothing
<221> VARIANT
<222> (571)...(571)
<223> Xaa = Proline or nothing
<400> 377
Xaa Asp Leu Thr Glu Glu Gln Ile Lys Ala Ala Gln Lys His Leu Glu
1               5                  10                  15
Glu Val Lys Thr Ser His Asn Gly Leu Asp Ser Leu Ser Ser His Glu
20                  25                  30
Gln Asp Tyr Pro Gly Asn Ala Lys Glu Met Lys Asp Leu Asp Lys Lys
35                  40                  45
Ile Glu Glu Lys Ile Ala Gly Ile Met Lys Gln Tyr Gly Val Lys Arg
50                  55                  60
Glu Ser Ile Val Val Asn Lys Glu Lys Asn Ala Ile Ile Tyr Pro His
65                  70                  75                  80
Gly Asp His His His Ala Asp Pro Ile Asp Glu His Lys Pro Val Gly
85                  90                  95
Ile Gly His Ser His Ser Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly
100                 105                 110
Val Ala Lys Lys Glu Gly Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr
115                 120                 125
Asn Val Val Asn Leu Leu Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe
130                 135                 140
Thr Leu Ala Asn Gly Gln Lys Arg Val Ser Phe Ser Phe Pro Pro Glu
145                 150                 155                 160
Leu Glu Lys Lys Leu Gly Ile Asn Met Leu Val Lys Leu Ile Thr Pro
165                 170                 175
Asp Gly Lys Val Leu Glu Lys Val Ser Gly Lys Val Phe Gly Glu Gly
180                 185                 190
Val Gly Asn Ile Ala Asn Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly
195                 200                 205
Gln Thr Phe Lys Tyr Thr Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser
210                 215                 220
Tyr Asp Gly Thr Phe Thr Val Pro Thr Ser Leu Ala Tyr Lys Met Ala
225                 230                 235                 240
Ser Gln Thr Ile Phe Tyr Pro Phe His Ala Gly Asp Thr Tyr Leu Arg
245                 250                 255
Val Asn Pro Gln Phe Ala Val Pro Lys Gly Thr Asp Ala Leu Val Arg
260                 265                 270
Val Phe Asp Glu Phe His Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys
275                 280                 285
Val Gly Glu Ile Lys Leu Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr
290                 295                 300
Arg Thr Ala Gly Asn Lys Ile Pro Val Thr Phe Met Ala Asn Ala Tyr
305                 310                 315                 320
Leu Asp Asn Gln Ser Thr Tyr Ile Val Glu Val Pro Ile Leu Glu Lys
325                 330                 335
Glu Asn Gln Thr Asp Lys Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn
340                 345                 350
Lys Ala Gln Glu Asn Ser Lys Leu Asp Glu Lys Val Glu Glu Pro Lys
355                 360                 365
Thr Ser Glu Lys Val Glu Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser
370                 375                 380
Thr Ser Asn Ser Thr Leu Glu Glu Val Pro Thr Val Asp Pro Val Gln
385                 390                 395                 400
Glu Lys Val Ala Lys Phe Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn
405                 410                 415
Val Leu Phe Asn Met Asp Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly
420                 425                 430
Glu Val Ile Lys Lys Asn Met Ala Asp Phe Thr Gly Glu Ala Pro Gln
435                 440                 445
Gly Asn Gly Glu Asn Lys Pro Ser Glu Asn Gly Lys Val Ser Thr Gly
450                 455                 460
Thr Val Glu Asn Gln Pro Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro
465                 470                 475                 480
Glu Ala Pro Asn Glu Lys Pro Val Lys Pro Glu Asn Ser Thr Asp Asn
485                 490                 495
Gly Met Leu Asn Pro Glu Gly Asn Val Gly Ser Asp Pro Met Leu Asp
500                 505                 510
Pro Ala Leu Glu Glu Ala Pro Ala Val Asp Pro Val Gln Glu Lys Leu
515                 520                 525
Glu Lys Phe Thr Ala Ser Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe
530                 535                 540
Asn Met Asp Gly Thr Ile Glu Leu Arg Leu Pro Ser Gly Glu Val Ile
545                 550                 555                 560
Lys Lys Asn Leu Ser Asp Phe Ile Ala Xaa Xaa Asp Ile Asp Ser Leu
565                 570                 575
Leu Lys Gln Leu Tyr Lys Leu Pro Leu Ser Gln Arg His Val Glu Ser
580                 585                 590
Asp Gly Leu Ile Phe Asp Pro Ala Gln Ile Thr Ser Arg Thr Ala Arg
595                 600                 605
Gly Val Ala Val Pro His Gly Asn His Tyr His Phe Ile Pro Tyr Glu
610                 615                 620
Gln Met Ser Glu Leu Glu Lys Arg Ile Ala Arg Ile Ile Pro Leu Arg
625                 630                 635                 640
Tyr Arg Ser Asn His Trp Val Pro Asp Ser Arg Pro Glu Glu Pro Ser
645                 650                 655
Pro Gln Pro Thr Pro Glu Pro Ser Pro Ser Pro Gln Pro Ala Pro Asn
660                 665                 670
Pro Gln Pro Ala Pro Ser Asn Pro Ile Asp Glu Lys Leu Val Lys Glu
675                 680                 685
Ala Val Arg Lys Val Gly Asp Gly Tyr Val Phe Glu Glu Asn Gly Val
690                 695                 700
Ser Arg Tyr Ile Pro Ala Lys Asn Leu Ser Ala Glu Thr Ala Ala Gly
705                 710                 715                 720
Ile Asp Ser Lys Leu Ala Lys Gln Glu Ser Leu Ser His Lys Leu Gly
725                 730                 735
Ala Lys Lys Thr Asp Leu Pro Ser Ser Asp Arg Glu Phe Tyr Asn Lys
740                 745                 750
Ala Tyr Asp Leu Leu Ala Arg Ile His Gln Asp Leu Leu Asp Asn Lys
755                 760                 765
Gly Arg Gln Val Asp Phe Glu Ala Leu Asp Asn Leu Leu Glu Arg Leu
770                 775                 780
Lys Asp Val Ser Ser Asp Lys Val Lys Leu Val Asp Asp Ile Leu Ala
785                 790                 795                 800
Phe Leu Ala Pro Ile Arg His Pro Glu Arg Leu Gly Lys Pro Asn Ala
805                 810                 815
Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val Ala Lys Leu Ala Gly
820                 825                 830
Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp Pro Arg Asp Ile Thr
835                 840                 845
Ser Asp Glu Gly Asp Ala Tyr Val Thr Pro His Met Thr His Ser His
850                 855                 860
Trp Ile Lys Lys Asp Ser Leu Ser Glu Ala Glu Arg Ala Ala Ala Gln
865                 870                 875                 880
Ala Tyr Ala Lys Glu Lys Gly Leu Thr Pro Pro Ser Thr Asp His Gln
885                 890                 895
Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala Glu Ala Ile Tyr Asn Arg
900                 905                 910
Val Lys Ala Ala Lys Lys Val Pro Leu Asp Arg Met Pro Tyr Asn Leu
915                 920                 925
Gln Tyr Thr Val Glu Val Lys Asn Gly Ser Leu Ile Ile Pro His Tyr
930                 935                 940
Asp His Tyr His Asn Ile Lys Phe Glu Trp Phe Asp Glu Gly Leu Tyr
945                 950                 955                 960
Glu Ala Pro Lys Gly Tyr Thr Leu Glu Asp Leu Leu Ala Thr Val Lys
965                 970                 975
Tyr Tyr Val Glu His Pro Asn Glu Arg Pro His Ser Asp Asn Gly Phe
980                 985                 990
Gly Asn Ala Ser Asp His Val
995
<210> 378
<211> 1378
<212> PRT
<213> Unknown
<220>
<223> NEW 26
<221> VARIANT
<222> (1)...(1)
<223> Xaa = Methionine or nothing
<221> VARIANT
<222> (570)...(570)
<223> Xaa = Glycine or nothing
<221> VARIANT
<222> (571)...(571)
<223> Xaa = Proline or nothing
<400> 378
Xaa Glu Asn Ile Ser Ser Leu Leu Arg Glu Leu Tyr Ala Lys Pro Leu
1               5                  10                  15
Ser Glu Arg His Val Glu Ser Asp Gly Leu Ile Phe Asp Pro Ala Gln
20                  25                  30
Ile Thr Ser Arg Thr Ala Arg Gly Val Ala Val Pro His Gly Asn His
35                  40                  45
Tyr His Phe Ile Pro Tyr Glu Gln Met Ser Glu Leu Glu Lys Arg Ile
50                  55                  60
Ala Arg Ile Ile Pro Leu Arg Tyr Arg Ser Asn His Trp Val Pro Asp
65                  70                  75                  80
Ser Arg Pro Glu Gln Pro Ser Pro Gln Ser Thr Pro Glu Pro Ser Pro
85                  90                  95
Ser Leu Gln Pro Ala Pro Asn Pro Gln Pro Ala Pro Ser Asn Pro Ile
100                 105                 110
Asp Glu Lys Leu Val Lys Glu Ala Val Arg Lys Val Gly Asp Gly Tyr
115                 120                 125
Val Phe Glu Glu Asn Gly Val Ser Arg Tyr Ile Pro Ala Lys Asp Leu
130                 135                 140
Ser Ala Glu Thr Ala Ala Gly Ile Asp Ser Lys Leu Ala Lys Gln Glu
145                 150                 155                 160
Ser Leu Ser His Lys Leu Gly Ala Lys Lys Thr Asp Leu Pro Ser Ser
165                 170                 175
Asp Arg Glu Phe Tyr Asn Lys Ala Tyr Asp Leu Leu Ala Arg Ile His
180                 185                 190
Gln Asp Leu Leu Asp Asn Lys Gly Arg Gln Val Asp Phe Glu Val Leu
195                 200                 205
Asp Asn Leu Leu Glu Arg Leu Lys Asp Val Ser Ser Asp Lys Val Lys
210                 215                 220
Leu Val Asp Asp Ile Leu Ala Phe Leu Ala Pro Ile Arg His Pro Glu
225                 230                 235                 240
Arg Leu Gly Lys Pro Asn Ala Gln Ile Thr Tyr Thr Asp Asp Glu Ile
245                 250                 255
Gln Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile
260                 265                 270
Phe Asp Pro Arg Asp Ile Thr Ser Asp Glu Gly Asp Ala Tyr Val Thr
275                 280                 285
Pro His Met Thr His Ser His Trp Ile Lys Lys Asp Ser Leu Ser Glu
290                 295                 300
Ala Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr
305                 310                 315                 320
Pro Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly
325                 330                 335
Ala Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu
340                 345                 350
Asp Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly
355                 360                 365
Ser Leu Ile Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe Glu
370                 375                 380
Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu
385                 390                 395                 400
Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg
405                 410                 415
Pro His Ser Asp Asn Gly Phe Gly Asn Ala Ser Asp His Val Arg Lys
420                 425                 430
Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp
435                 440                 445
Glu Val Ser Glu Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His
450                 455                 460
Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp
465                 470                 475                 480
Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu
485                 490                 495
Ile Pro Gln Val Glu Asn Ser Val Ile Asn Ala Lys Ile Ala Asp Ala
500                 505                 510
Glu Ala Leu Leu Glu Lys Val Thr Asp Pro Ser Ile Arg Gln Asn Ala
515                 520                 525
Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr Lys
530                 535                 540
Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu Leu
545                 550                 555                 560
Lys Glu Ser Gln Pro Ala Pro Ile Gln Xaa Xaa Asn Met Gln Pro Ser
565                 570                 575
Gln Leu Ser Tyr Ser Ser Thr Ala Ser Asp Asn Asn Thr Gln Ser Val
580                 585                 590
Ala Lys Gly Ser Thr Ser Lys Pro Ala Asn Lys Ser Glu Asn Leu Gln
595                 600                 605
Ser Leu Leu Lys Glu Leu Tyr Asp Ser Pro Ser Ala Gln Arg Tyr Ser
610                 615                 620
Glu Ser Asp Gly Leu Val Phe Asp Pro Ala Lys Ile Ile Ser Arg Thr
625                 630                 635                 640
Pro Asn Gly Val Ala Ile Pro His Gly Asp His Tyr His Phe Ile Pro
645                 650                 655
Tyr Ser Lys Leu Ser Ala Leu Glu Glu Lys Ile Ala Arg Met Val Pro
660                 665                 670
Ile Ser Gly Thr Gly Ser Thr Val Ser Thr Asn Ala Lys Pro Asn Glu
675                 680                 685
Val Val Ser Ser Leu Gly Ser Leu Ser Ser Asn Pro Ser Ser Leu Thr
690                 695                 700
Thr Ser Lys Glu Leu Ser Ser Ala Ser Asp Gly Tyr Ile Phe Asn Pro
705                 710                 715                 720
Lys Asp Ile Val Glu Glu Thr Ala Thr Ala Tyr Ile Val Arg His Gly
725                 730                 735
Asp His Phe His Tyr Ile Pro Lys Ser Asn Gln Ile Gly Gln Pro Thr
740                 745                 750
Leu Pro Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu Pro Ile Asn
755                 760                 765
Pro Gly Thr Ser His Glu Lys His Glu Glu Asp Gly Tyr Gly Phe Asp
770                 775                 780
Ala Asn Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe Val Met Ser His
785                 790                 795                 800
Gly Asp His Asn His Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu Gln
805                 810                 815
Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His Asn
820                 825                 830
Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn Ala
835                 840                 845
Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly
850                 855                 860
Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys
865                 870                 875                 880
Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His His Ala Asp
885                 890                 895
Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn
900                 905                 910
Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn
915                 920                 925
Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys
930                 935                 940
Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys
945                 950                 955                 960
Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile
965                 970                 975
Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys
980                 985                 990
Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe
995                 1000                1005
Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile
1010                1015                1020
Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val
1025                1030                1035                1040
Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro
1045                1050                1055
Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val
1060                1065                1070
Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly
1075                1080                1085
Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro
1090                1095                1100
Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile
1105                1110                1115                1120
Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr
1125                1130                1135
Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro
1140                1145                1150
Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys
1155                1160                1165
Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys
1170                1175                1180
Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu
1185                1190                1195                1200
Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala
1205                1210                1215
Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly
1220                1225                1230
Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met
1235                1240                1245
Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro
1250                1255                1260
Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr
1265                1270                1275                1280
Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro
1285                1290                1295
Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly
1300                1305                1310
Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro
1315                1320                1325
Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr
1330                1335                1340
Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu
1345                1350                1355                1360
Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe
1365                1370                1375
Ile Ala
<210> 379
<211> 1152
<212> PRT
<213> Unknown
<220>
<223> NEW 27
<221> VARIANT
<222> (1)...(1)
<223> Xaa = Methionine or nothing
<221> VARIANT
<222> (344)...(344)
<223> Xaa = Glycine or nothing
<221> VARIANT
<222> (345)...(345)
<223> Xaa = Proline or nothing
<400> 379
Xaa Asp Ile Leu Ala Phe Leu Ala Pro Ile Arg His Pro Glu Arg Leu
1               5                  10                  15
Gly Lys Pro Asn Ala Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln Val
20                  25                  30
Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe Asp
35                  40                  45
Pro Arg Asp Ile Thr Ser Asp Glu Gly Asp Ala Tyr Val Thr Pro His
50                  55                  60
Met Thr His Ser His Trp Ile Lys Lys Asp Ser Leu Ser Glu Ala Glu
65                  70                  75                  80
Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr Pro Pro
85                  90                  95
Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala Glu
100                 105                 110
Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu Asp Arg
115                 120                 125
Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly Ser Leu
130                 135                 140
Ile Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe Glu Trp Phe
145                 150                 155                 160
Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu Asp Leu
165                 170                 175
Leu Ala Thr Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg Pro His
180                 185                 190
Ser Asp Asn Gly Phe Gly Asn Ala Ser Asp His Val Arg Lys Asn Lys
195                 200                 205
Ala Asp Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp Glu Val
210                 215                 220
Ser Glu Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His Ala Gly
225                 230                 235                 240
Leu Asn Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp Thr Glu
245                 250                 255
Glu Thr Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu Ile Pro
260                 265                 270
Gln Val Glu Asn Ser Val Ile Asn Ala Lys Ile Ala Asp Ala Glu Ala
275                 280                 285
Leu Leu Glu Lys Val Thr Asp Pro Ser Ile Arg Gln Asn Ala Met Glu
290                 295                 300
Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr Lys Asp Asn
305                 310                 315                 320
Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu Leu Lys Glu
325                 330                 335
Ser Gln Pro Ala Pro Ile Gln Xaa Xaa Asn Met Gln Pro Ser Gln Leu
340                 345                 350
Ser Tyr Ser Ser Thr Ala Ser Asp Asn Asn Thr Gln Ser Val Ala Lys
355                 360                 365
Gly Ser Thr Ser Lys Pro Ala Asn Lys Ser Glu Asn Leu Gln Ser Leu
370                 375                 380
Leu Lys Glu Leu Tyr Asp Ser Pro Ser Ala Gln Arg Tyr Ser Glu Ser
385                 390                 395                 400
Asp Gly Leu Val Phe Asp Pro Ala Lys Ile Ile Ser Arg Thr Pro Asn
405                 410                 415
Gly Val Ala Ile Pro His Gly Asp His Tyr His Phe Ile Pro Tyr Ser
420                 425                 430
Lys Leu Ser Ala Leu Glu Glu Lys Ile Ala Arg Met Val Pro Ile Ser
435                 440                 445
Gly Thr Gly Ser Thr Val Ser Thr Asn Ala Lys Pro Asn Glu Val Val
450                 455                 460
Ser Ser Leu Gly Ser Leu Ser Ser Asn Pro Ser Ser Leu Thr Thr Ser
465                 470                 475                 480
Lys Glu Leu Ser Ser Ala Ser Asp Gly Tyr Ile Phe Asn Pro Lys Asp
485                 490                 495
Ile Val Glu Glu Thr Ala Thr Ala Tyr Ile Val Arg His Gly Asp His
500                 505                 510
Phe His Tyr Ile Pro Lys Ser Asn Gln Ile Gly Gln Pro Thr Leu Pro
515                 520                 525
Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser Leu Pro Ile Asn Pro Gly
530                 535                 540
Thr Ser His Glu Lys His Glu Glu Asp Gly Tyr Gly Phe Asp Ala Asn
545                 550                 555                 560
Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe Val Met Ser His Gly Asp
565                 570                 575
His Asn His Tyr Phe Phe Lys Lys Asp Leu Thr Glu Glu Gln Ile Lys
580                 585                 590
Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His Asn Gly Leu
595                 600                 605
Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn Ala Lys Glu
610                 615                 620
Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala Gly Ile Met
625                 630                 635                 640
Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn Lys Glu Lys
645                 650                 655
Asn Ala Ile Ile Tyr Pro His Gly Asp His His His Ala Asp Pro Ile
660                 665                 670
Asp Glu His Lys Pro Val Gly Ile Gly His Ser His Ser Asn Tyr Glu
675                 680                 685
Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly Asn Lys Val
690                 695                 700
Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu Lys Asn Ser
705                 710                 715                 720
Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln Lys Arg Val
725                 730                 735
Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly Ile Asn Met
740                 745                 750
Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu Lys Val Ser
755                 760                 765
Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn Phe Glu Leu
770                 775                 780
Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr Ile Ala Ser
785                 790                 795                 800
Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr Val Pro Thr
805                 810                 815
Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr Pro Phe His
820                 825                 830
Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala Val Pro Lys
835                 840                 845
Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His Gly Asn Ala
850                 855                 860
Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu Pro Ile Pro
865                 870                 875                 880
Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys Ile Pro Val
885                 890                 895
Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr Tyr Ile Val
900                 905                 910
Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys Pro Ser Ile
915                 920                 925
Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser Lys Leu Asp
930                 935                 940
Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu Lys Glu Lys
945                 950                 955                 960
Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu Glu Glu Val
965                 970                 975
Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe Ala Glu Ser
980                 985                 990
Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp Gly Thr Ile
995                 1000                1005
Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Met Ala Asp
1010                1015                1020
Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys Pro Ser Glu
1025                1030                1035                1040
Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro Thr Glu Asn
1045                1050                1055
Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys Pro Val Lys
1060                1065                1070
Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu Gly Asn Val
1075                1080                1085
Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala Pro Ala Val
1090                1095                1100
Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser Tyr Gly Leu
1105                1110                1115                1120
Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile Glu Leu Arg
1125                1130                1135
Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp Phe Ile Ala
1140                1145                1150
<210> 380
<211> 1139
<212> PRT
<213> Unknown
<220>
<223> NEW 28
<221> VARIANT
<222> (1)...(1)
<223> Xaa = Methionine or nothing
<221> VARIANT
<222> (570)...(570)
<223> Xaa = Glycine or nothing
<221> VARIANT
<222> (571)...(571)
<223> Xaa = Proline or nothing
<400> 380
Xaa Glu Asn Ile Ser Ser Leu Leu Arg Glu Leu Tyr Ala Lys Pro Leu
1               5                  10                  15
Ser Glu Arg His Val Glu Ser Asp Gly Leu Ile Phe Asp Pro Ala Gln
20                  25                  30
Ile Thr Ser Arg Thr Ala Arg Gly Val Ala Val Pro His Gly Asn His
35                  40                  45
Tyr His Phe Ile Pro Tyr Glu Gln Met Ser Glu Leu Glu Lys Arg Ile
50                  55                  60
Ala Arg Ile Ile Pro Leu Arg Tyr Arg Ser Asn His Trp Val Pro Asp
65                  70                  75                  80
Ser Arg Pro Glu Gln Pro Ser Pro Gln Ser Thr Pro Glu Pro Ser Pro
85                  90                  95
Ser Leu Gln Pro Ala Pro Asn Pro Gln Pro Ala Pro Ser Asn Pro Ile
100                 105                 110
Asp Glu Lys Leu Val Lys Glu Ala Val Arg Lys Val Gly Asp Gly Tyr
115                 120                 125
Val Phe Glu Glu Asn Gly Val Ser Arg Tyr Ile Pro Ala Lys Asp Leu
130                 135                 140
Ser Ala Glu Thr Ala Ala Gly Ile Asp Ser Lys Leu Ala Lys Gln Glu
145                 150                 155                 160
Ser Leu Ser His Lys Leu Gly Ala Lys Lys Thr Asp Leu Pro Ser Ser
165                 170                 175
Asp Arg Glu Phe Tyr Asn Lys Ala Tyr Asp Leu Leu Ala Arg Ile His
180                 185                 190
Gln Asp Leu Leu Asp Asn Lys Gly Arg Gln Val Asp Phe Glu Val Leu
195                 200                 205
Asp Asn Leu Leu Glu Arg Leu Lys Asp Val Ser Ser Asp Lys Val Lys
210                 215                 220
Leu Val Asp Asp Ile Leu Ala Phe Leu Ala Pro Ile Arg His Pro Glu
225                 230                 235                 240
Arg Leu Gly Lys Pro Asn Ala Gln Ile Thr Tyr Thr Asp Asp Glu Ile
245                 250                 255
Gln Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile
260                 265                 270
Phe Asp Pro Arg Asp Ile Thr Ser Asp Glu Gly Asp Ala Tyr Val Thr
275                 280                 285
Pro His Met Thr His Ser His Trp Ile Lys Lys Asp Ser Leu Ser Glu
290                 295                 300
Ala Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr
305                 310                 315                 320
Pro Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly
325                 330                 335
Ala Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu
340                 345                 350
Asp Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly
355                 360                 365
Ser Leu Ile Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe Glu
370                 375                 380
Trp Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Ser Leu Glu
385                 390                 395                 400
Asp Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg
405                 410                 415
Pro His Ser Asp Asn Gly Phe Gly Asn Ala Ser Asp His Val Arg Lys
420                 425                 430
Asn Lys Ala Asp Gln Asp Ser Lys Pro Asp Glu Asp Lys Glu His Asp
435                 440                 445
Glu Val Ser Glu Pro Thr His Pro Glu Ser Asp Glu Lys Glu Asn His
450                 455                 460
Ala Gly Leu Asn Pro Ser Ala Asp Asn Leu Tyr Lys Pro Ser Thr Asp
465                 470                 475                 480
Thr Glu Glu Thr Glu Glu Glu Ala Glu Asp Thr Thr Asp Glu Ala Glu
485                 490                 495
Ile Pro Gln Val Glu Asn Ser Val Ile Asn Ala Lys Ile Ala Asp Ala
500                 505                 510
Glu Ala Leu Leu Glu Lys Val Thr Asp Pro Ser Ile Arg Gln Asn Ala
515                 520                 525
Met Glu Thr Leu Thr Gly Leu Lys Ser Ser Leu Leu Leu Gly Thr Lys
530                 535                 540
Asp Asn Asn Thr Ile Ser Ala Glu Val Asp Ser Leu Leu Ala Leu Leu
545                 550                 555                 560
Lys Glu Ser Gln Pro Ala Pro Ile Gln Xaa Xaa Asp Leu Thr Glu Glu
565                 570                 575
Gln Ile Lys Ala Ala Gln Lys His Leu Glu Glu Val Lys Thr Ser His
580                 585                 590
Asn Gly Leu Asp Ser Leu Ser Ser His Glu Gln Asp Tyr Pro Gly Asn
595                 600                 605
Ala Lys Glu Met Lys Asp Leu Asp Lys Lys Ile Glu Glu Lys Ile Ala
610                 615                 620
Gly Ile Met Lys Gln Tyr Gly Val Lys Arg Glu Ser Ile Val Val Asn
625                 630                 635                 640
Lys Glu Lys Asn Ala Ile Ile Tyr Pro His Gly Asp His His His Ala
645                 650                 655
Asp Pro Ile Asp Glu His Lys Pro Val Gly Ile Gly His Ser His Ser
660                 665                 670
Asn Tyr Glu Leu Phe Lys Pro Glu Glu Gly Val Ala Lys Lys Glu Gly
675                 680                 685
Asn Lys Val Tyr Thr Gly Glu Glu Leu Thr Asn Val Val Asn Leu Leu
690                 695                 700
Lys Asn Ser Thr Phe Asn Asn Gln Asn Phe Thr Leu Ala Asn Gly Gln
705                 710                 715                 720
Lys Arg Val Ser Phe Ser Phe Pro Pro Glu Leu Glu Lys Lys Leu Gly
725                 730                 735
Ile Asn Met Leu Val Lys Leu Ile Thr Pro Asp Gly Lys Val Leu Glu
740                 745                 750
Lys Val Ser Gly Lys Val Phe Gly Glu Gly Val Gly Asn Ile Ala Asn
755                 760                 765
Phe Glu Leu Asp Gln Pro Tyr Leu Pro Gly Gln Thr Phe Lys Tyr Thr
770                 775                 780
Ile Ala Ser Lys Asp Tyr Pro Glu Val Ser Tyr Asp Gly Thr Phe Thr
785                 790                 795                 800
Val Pro Thr Ser Leu Ala Tyr Lys Met Ala Ser Gln Thr Ile Phe Tyr
805                 810                 815
Pro Phe His Ala Gly Asp Thr Tyr Leu Arg Val Asn Pro Gln Phe Ala
820                 825                 830
Val Pro Lys Gly Thr Asp Ala Leu Val Arg Val Phe Asp Glu Phe His
835                 840                 845
Gly Asn Ala Tyr Leu Glu Asn Asn Tyr Lys Val Gly Glu Ile Lys Leu
850                 855                 860
Pro Ile Pro Lys Leu Asn Gln Gly Thr Thr Arg Thr Ala Gly Asn Lys
865                 870                 875                 880
Ile Pro Val Thr Phe Met Ala Asn Ala Tyr Leu Asp Asn Gln Ser Thr
885                 890                 895
Tyr Ile Val Glu Val Pro Ile Leu Glu Lys Glu Asn Gln Thr Asp Lys
900                 905                 910
Pro Ser Ile Leu Pro Gln Phe Lys Arg Asn Lys Ala Gln Glu Asn Ser
915                 920                 925
Lys Leu Asp Glu Lys Val Glu Glu Pro Lys Thr Ser Glu Lys Val Glu
930                 935                 940
Lys Glu Lys Leu Ser Glu Thr Gly Asn Ser Thr Ser Asn Ser Thr Leu
945                 950                 955                 960
Glu Glu Val Pro Thr Val Asp Pro Val Gln Glu Lys Val Ala Lys Phe
965                 970                 975
Ala Glu Ser Tyr Gly Met Lys Leu Glu Asn Val Leu Phe Asn Met Asp
980                 985                 990
Gly Thr Ile Glu Leu Tyr Leu Pro Ser Gly Glu Val Ile Lys Lys Asn
995                 1000                1005
Met Ala Asp Phe Thr Gly Glu Ala Pro Gln Gly Asn Gly Glu Asn Lys
1010                1015                1020
Pro Ser Glu Asn Gly Lys Val Ser Thr Gly Thr Val Glu Asn Gln Pro
1025                1030                1035                1040
Thr Glu Asn Lys Pro Ala Asp Ser Leu Pro Glu Ala Pro Asn Glu Lys
1045                1050                1055
Pro Val Lys Pro Glu Asn Ser Thr Asp Asn Gly Met Leu Asn Pro Glu
1060                1065                1070
Gly Asn Val Gly Ser Asp Pro Met Leu Asp Pro Ala Leu Glu Glu Ala
1075                1080                1085
Pro Ala Val Asp Pro Val Gln Glu Lys Leu Glu Lys Phe Thr Ala Ser
1090                1095                1100
Tyr Gly Leu Gly Leu Asp Ser Val Ile Phe Asn Met Asp Gly Thr Ile
1105                1110                1115                1120
Glu Leu Arg Leu Pro Ser Gly Glu Val Ile Lys Lys Asn Leu Ser Asp
1125                1130                1135
Phe Ile Ala
<210> 381
<211> 1238
<212> PRT
<213> Unknown
<220>
<223> NEW 29
<221> VARIANT
<222> (1)...(1)
<223> Xaa = Methionine or nothing
<221> VARIANT
<222> (430)...(430)
<223> Xaa = Glycine or nothing
<221> VARIANT
<222> (431)...(431)
<223> Xaa = Proline or nothing
<400> 381
Xaa Asp Ile Asp Ser Leu Leu Lys Gln Leu Tyr Lys Leu Pro Leu Ser
1               5                  10                  15
Gln Arg His Val Glu Ser Asp Gly Leu Ile Phe Asp Pro Ala Gln Ile
20                  25                  30
Thr Ser Arg Thr Ala Arg Gly Val Ala Val Pro His Gly Asn His Tyr
35                  40                  45
His Phe Ile Pro Tyr Glu Gln Met Ser Glu Leu Glu Lys Arg Ile Ala
50                  55                  60
Arg Ile Ile Pro Leu Arg Tyr Arg Ser Asn His Trp Val Pro Asp Ser
65                  70                  75                  80
Arg Pro Glu Glu Pro Ser Pro Gln Pro Thr Pro Glu Pro Ser Pro Ser
85                  90                  95
Pro Gln Pro Ala Pro Asn Pro Gln Pro Ala Pro Ser Asn Pro Ile Asp
100                 105                 110
Glu Lys Leu Val Lys Glu Ala Val Arg Lys Val Gly Asp Gly Tyr Val
115                 120                 125
Phe Glu Glu Asn Gly Val Ser Arg Tyr Ile Pro Ala Lys Asn Leu Ser
130                 135                 140
Ala Glu Thr Ala Ala Gly Ile Asp Ser Lys Leu Ala Lys Gln Glu Ser
145                 150                 155                 160
Leu Ser His Lys Leu Gly Ala Lys Lys Thr Asp Leu Pro Ser Ser Asp
165                 170                 175
Arg Glu Phe Tyr Asn Lys Ala Tyr Asp Leu Leu Ala Arg Ile His Gln
180                 185                 190
Asp Leu Leu Asp Asn Lys Gly Arg Gln Val Asp Phe Glu Ala Leu Asp
195                 200                 205
Asn Leu Leu Glu Arg Leu Lys Asp Val Ser Ser Asp Lys Val Lys Leu
210                 215                 220
Val Asp Asp Ile Leu Ala Phe Leu Ala Pro Ile Arg His Pro Glu Arg
225                 230                 235                 240
Leu Gly Lys Pro Asn Ala Gln Ile Thr Tyr Thr Asp Asp Glu Ile Gln
245                 250                 255
Val Ala Lys Leu Ala Gly Lys Tyr Thr Thr Glu Asp Gly Tyr Ile Phe
260                 265                 270
Asp Pro Arg Asp Ile Thr Ser Asp Glu Gly Asp Ala Tyr Val Thr Pro
275                 280                 285
His Met Thr His Ser His Trp Ile Lys Lys Asp Ser Leu Ser Glu Ala
290                 295                 300
Glu Arg Ala Ala Ala Gln Ala Tyr Ala Lys Glu Lys Gly Leu Thr Pro
305                 310                 315                 320
Pro Ser Thr Asp His Gln Asp Ser Gly Asn Thr Glu Ala Lys Gly Ala
325                 330                 335
Glu Ala Ile Tyr Asn Arg Val Lys Ala Ala Lys Lys Val Pro Leu Asp
340                 345                 350
Arg Met Pro Tyr Asn Leu Gln Tyr Thr Val Glu Val Lys Asn Gly Ser
355                 360                 365
Leu Ile Ile Pro His Tyr Asp His Tyr His Asn Ile Lys Phe Glu Trp
370                 375                 380
Phe Asp Glu Gly Leu Tyr Glu Ala Pro Lys Gly Tyr Thr Leu Glu Asp
385                 390                 395                 400
Leu Leu Ala Thr Val Lys Tyr Tyr Val Glu His Pro Asn Glu Arg Pro
405                 410                 415
His Ser Asp Asn Gly Phe Gly Asn Ala Ser Asp His Val Xaa Xaa Asn
420                 425                 430
Met Gln Pro Ser Gln Leu Ser Tyr Ser Ser Thr Ala Ser Asp Asn Asn
435                 440                 445
Thr Gln Ser Val Ala Lys Gly Ser Thr Ser Lys Pro Ala Asn Lys Ser
450                 455                 460
Glu Asn Leu Gln Ser Leu Leu Lys Glu Leu Tyr Asp Ser Pro Ser Ala
465                 470                 475                 480
Gln Arg Tyr Ser Glu Ser Asp Gly Leu Val Phe Asp Pro Ala Lys Ile
485                 490                 495
Ile Ser Arg Thr Pro Asn Gly Val Ala Ile Pro His Gly Asp His Tyr
500                 505                 510
His Phe Ile Pro Tyr Ser Lys Leu Ser Ala Leu Glu Glu Lys Ile Ala
515                 520                 525
Arg Met Val Pro Ile Ser Gly Thr Gly Ser Thr Val Ser Thr Asn Ala
530                 535                 540
Lys Pro Asn Glu Val Val Ser Ser Leu Gly Ser Leu Ser Ser Asn Pro
545                 550                 555                 560
Ser Ser Leu Thr Thr Ser Lys Glu Leu Ser Ser Ala Ser Asp Gly Tyr
565                 570                 575
Ile Phe Asn Pro Lys Asp Ile Val Glu Glu Thr Ala Thr Ala Tyr Ile
580                 585                 590
Val Arg His Gly Asp His Phe His Tyr Ile Pro Lys Ser Asn Gln Ile
595                 600                 605
Gly Gln Pro Thr Leu Pro Asn Asn Ser Leu Ala Thr Pro Ser Pro Ser
610                 615                 620
Leu Pro Ile Asn Pro Gly Thr Ser His Glu Lys His Glu Glu Asp Gly
625                 630                 635                 640
Tyr Gly Phe Asp Ala Asn Arg Ile Ile Ala Glu Asp Glu Ser Gly Phe
645                 650                 655
Val Met Ser His Gly Asp His Asn His Tyr Phe Phe Lys Lys Asp Leu
660                 665                 670
Thr Glu Glu Gln Ile Lys Ala Ala Gln 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††††††††㘠㔳††††††††㘠〴戼㹲倊敨䤠敬倠潲吠特匠牥䰠獹䰠略匠牥䄠慬䰠略䜠畬䜠畬䰠獹䤠敬䄠慬䄠杲戼㹲 †††††††㘠㔴††††††††㘠〵††††††††㘠㔵戼㹲䴊瑥嘠污倠潲䤠敬匠牥䜠祬吠牨䜠祬匠牥吠牨嘠污匠牥吠牨䄠湳䄠慬䰠獹戼㹲 †††††㘠〶††††††††㘠㔶††††††††㘠〷戼㹲倊潲䄠湳䜠畬嘠污嘠污匠牥匠牥䰠略䜠祬匠牥䰠略匠牥匠牥䄠湳倠潲匠牥戼㹲 †††㘠㔷††††††††㘠〸††††††††㘠㔸戼㹲匊牥䰠略吠牨吠牨匠牥䰠獹䜠畬䰠略匠牥匠牥䄠慬匠牥䄠灳䜠祬吠特䤠敬戼㹲 †㘠〹††††††††㘠㔹††††††††㜠〰戼㹲倊敨䄠湳倠潲䰠獹䄠灳䤠敬嘠污䜠畬䜠畬吠牨䄠慬吠牨䄠慬吠特䤠敬嘠污戼㹲㜊㔰††††††††㜠〱††††††††㜠㔱††††††††㜠〲戼㹲䄊杲䠠獩䜠祬䄠灳䠠獩倠敨䠠獩吠特䤠敬倠潲䰠獹匠牥䄠湳䜠湬䤠敬䜠祬戼㹲 †††††††㜠㔲††††††††㜠〳††††††††㜠㔳戼㹲䜊湬倠潲吠牨䰠略倠潲䄠湳䄠湳匠牥䰠略䄠慬吠牨倠潲匠牥倠潲匠牥䰠略戼㹲 †††††㜠〴††††††††㜠㔴††††††††㜠〵戼㹲倊潲䤠敬䄠湳倠潲䜠祬吠牨匠牥䠠獩䜠畬䰠獹䠠獩䜠畬䜠畬䄠灳䜠祬吠特戼㹲 †††㜠㔵††††††††㜠〶††††††††㜠㔶戼㹲䜊祬倠敨䄠灳䄠慬䄠湳䄠杲䤠敬䤠敬䄠慬䜠畬䄠灳䜠畬匠牥䜠祬倠敨嘠污戼㹲 †㜠〷††††††††㜠㔷††††††††㜠〸戼㹲䴊瑥匠牥䠠獩䜠祬䄠灳䠠獩䄠湳䠠獩吠特倠敨倠敨䰠獹䰠獹䄠灳䰠略吠牨戼㹲㜊㔸††††††††㜠〹††††††††㜠㔹††††††††㠠〰戼㹲䜊畬䜠畬䜠湬䤠敬䰠獹䄠慬䄠慬䜠湬䰠獹䠠獩䰠略䜠畬䜠畬嘠污䰠獹吠牨戼㹲 †††††††㠠㔰††††††††㠠〱††††††††㠠㔱戼㹲匊牥䠠獩䄠湳䜠祬䰠略䄠灳匠牥䰠略匠牥匠牥䠠獩䜠畬䜠湬䄠灳吠特倠潲戼㹲 †††††㠠〲††††††††㠠㔲††††††††㠠〳戼㹲䜊祬䄠湳䄠慬䰠獹䜠畬䴠瑥䰠獹䄠灳䰠略䄠灳䰠獹䰠獹䤠敬䜠畬䜠畬䰠獹戼㹲 †††㠠㔳††††††††㠠〴††††††††㠠㔴戼㹲䤊敬䄠慬䜠祬䤠敬䴠瑥䰠獹䜠湬吠特䜠祬嘠污䰠獹䄠杲䜠畬匠牥䤠敬嘠污戼㹲 †㠠〵††††††††㠠㔵††††††††㠠〶戼㹲嘊污䄠湳䰠獹䜠畬䰠獹䄠湳䄠慬䤠敬䤠敬吠特倠潲䠠獩䜠祬䄠灳䠠獩䠠獩戼㹲㠊㔶††††††††㠠〷††††††††㠠㔷††††††††㠠〸戼㹲䠊獩䄠慬䄠灳倠潲䤠敬䄠灳䜠畬䠠獩䰠獹倠潲嘠污䜠祬䤠敬䜠祬䠠獩匠牥戼㹲 †††††††㠠㔸††††††††㠠〹††††††††㠠㔹戼㹲䠊獩匠牥䄠湳吠特䜠畬䰠略倠敨䰠獹倠潲䜠畬䜠畬䜠祬嘠污䄠慬䰠獹䰠獹戼㹲 †††††㤠〰††††††††㤠㔰††††††††㤠〱戼㹲䜊畬䜠祬䄠湳䰠獹嘠污吠特吠牨䜠祬䜠畬䜠畬䰠略吠牨䄠湳嘠污嘠污䄠湳戼㹲 †††㤠㔱††††††††㤠〲††††††††㤠㔲戼㹲䰊略䰠略䰠獹䄠湳匠牥吠牨倠敨䄠湳䄠湳䜠湬䄠湳倠敨吠牨䰠略䄠慬䄠湳戼㹲 †㤠〳††††††††㤠㔳††††††††㤠〴戼㹲䜊祬䜠湬䰠獹䄠杲嘠污匠牥倠敨匠牥倠敨倠潲倠潲䜠畬䰠略䜠畬䰠獹䰠獹戼㹲㤊㔴††††††††㤠〵††††††††㤠㔵††††††††㤠〶戼㹲䰊略䜠祬䤠敬䄠湳䴠瑥䰠略嘠污䰠獹䰠略䤠敬吠牨倠潲䄠灳䜠祬䰠獹嘠污戼㹲 †††††††㤠㔶††††††††㤠〷††††††††㤠㔷戼㹲䰊略䜠畬䰠獹嘠污匠牥䜠祬䰠獹嘠污倠敨䜠祬䜠畬䜠祬嘠污䜠祬䄠湳䤠敬戼㹲 †††††㤠〸††††††††㤠㔸††††††††㤠〹戼㹲䄊慬䄠湳倠敨䜠畬䰠略䄠灳䜠湬倠潲吠特䰠略倠潲䜠祬䜠湬吠牨倠敨䰠獹戼㹲 †††㤠㔹††††††††ㄠ〰‰†††††††ㄠ〰㰵牢ਾ祔⁲桔⁲汉⁥汁⁡敓⁲祌⁳獁⁰祔⁲牐⁯汇⁵慖⁬敓⁲祔⁲獁⁰汇⁹桔㱲牢ਾ††〱〱††††††††〱㔱††††††††〱〲戼㹲倊敨吠牨嘠污倠潲吠牨匠牥䰠略䄠慬吠特䰠獹䴠瑥䄠慬匠牥䜠湬吠牨䤠敬戼㹲ㄊ㈰‵†††††††ㄠ㌰‰†††††††ㄠ㌰‵†††††††ㄠ㐰㰰牢ਾ桐⁥祔⁲牐⁯桐⁥楈⁳汁⁡汇⁹獁⁰桔⁲祔⁲敌⁵牁⁧慖⁬獁⁮牐⁯汇㱮牢ਾ††††††††〱㔴††††††††〱〵††††††††〱㔵戼㹲倊敨䄠慬嘠污倠潲䰠獹䜠祬吠牨䄠灳䄠慬䰠略嘠污䄠杲嘠污倠敨䄠灳䜠畬戼㹲 †††††ㄠ㘰‰†††††††ㄠ㘰‵†††††††ㄠ㜰㰰牢ਾ桐⁥楈⁳汇⁹獁⁮汁⁡祔⁲敌⁵汇⁵獁⁮獁⁮祔⁲祌⁳慖⁬汇⁹汇⁵汉㱥牢ਾ††††〱㔷††††††††〱〸††††††††〱㔸戼㹲䰊獹䰠略倠潲䤠敬倠潲䰠獹䰠略䄠湳䜠湬䜠祬吠牨吠牨䄠杲吠牨䄠慬䜠祬戼㹲 †ㄠ㤰‰†††††††ㄠ㤰‵†††††††ㄠ〱㰰牢ਾ獁⁮祌⁳汉⁥牐⁯慖⁬桔⁲桐⁥敍⁴汁⁡獁⁮汁⁡祔⁲敌⁵獁⁰獁⁮汇㱮牢ਾㄱ㔰††††††††ㄱ〱††††††††ㄱ㔱††††††††ㄱ〲戼㹲匊牥吠牨吠特䤠敬嘠污䜠畬嘠污倠潲䤠敬䰠略䜠畬䰠獹䜠畬䄠湳䜠湬吠牨戼㹲 †††††††ㄠ㈱‵†††††††ㄠ㌱‰†††††††ㄠ㌱㰵牢ਾ獁⁰祌⁳牐⁯敓⁲汉⁥敌⁵牐⁯汇⁮桐⁥祌⁳牁⁧獁⁮祌⁳汁⁡汇⁮汇㱵牢ਾ††††††ㄱ〴††††††††ㄱ㔴††††††††ㄱ〵戼㹲䄊湳匠牥䰠獹䰠略䄠灳䜠畬䰠獹嘠污䜠畬䜠畬倠潲䰠獹吠牨匠牥䜠畬䰠獹戼㹲 †††ㄠ㔱‵†††††††ㄠ㘱‰†††††††ㄠ㘱㰵牢ਾ慖⁬汇⁵祌⁳汇⁵祌⁳敌⁵敓⁲汇⁵桔⁲汇⁹獁⁮敓⁲桔⁲敓⁲獁⁮敓㱲牢ਾ††ㄱ〷††††††††ㄱ㔷††††††††ㄱ〸戼㹲吊牨䰠略䜠畬䜠畬嘠污倠潲吠牨嘠污䄠灳倠潲嘠污䜠湬䜠畬䰠獹嘠污䄠慬戼㹲ㄊ㠱‵†††††††ㄠ㤱‰†††††††ㄠ㤱‵†††††††ㄠ〲㰰牢ਾ祌⁳桐⁥汁⁡汇⁵敓⁲祔⁲汇⁹敍⁴祌⁳敌⁵汇⁵獁⁮慖⁬敌⁵桐⁥獁㱮牢ਾ††††††††㈱㔰††††††††㈱〱††††††††㈱㔱戼㹲䴊瑥䄠灳䜠祬吠牨䤠敬䜠畬䰠略吠特䰠略倠潲匠牥䜠祬䜠畬嘠污䤠敬䰠獹戼㹲 †††††ㄠ㈲‰†††††††ㄠ㈲‵†††††††ㄠ㌲㰰牢ਾ祌⁳獁⁮敍⁴汁⁡獁⁰桐⁥桔⁲汇⁹汇⁵汁⁡牐⁯汇⁮汇⁹獁⁮汇⁹汇㱵牢ਾ††††㈱㔳††††††††㈱〴††††††††㈱㔴戼㹲䄊湳䰠獹倠潲匠牥䜠畬䄠湳䜠祬䰠獹嘠污匠牥吠牨䜠祬吠牨嘠污䜠畬䄠湳戼㹲 †ㄠ㔲‰†††††††ㄠ㔲‵†††††††ㄠ㘲㰰牢ਾ汇⁮牐⁯桔⁲汇⁵獁⁮祌⁳牐⁯汁⁡獁⁰敓⁲敌⁵牐⁯汇⁵汁⁡牐⁯獁㱮牢ਾ㈱㔶††††††††㈱〷††††††††㈱㔷††††††††㈱〸戼㹲䜊畬䰠獹倠潲嘠污䰠獹倠潲䜠畬䄠湳匠牥吠牨䄠灳䄠湳䜠祬䴠瑥䰠略䄠湳戼㹲 †††††††ㄠ㠲‵†††††††ㄠ㤲‰†††††††ㄠ㤲㰵牢ਾ牐⁯汇⁵汇⁹獁⁮慖⁬汇⁹敓⁲獁⁰牐⁯敍⁴敌⁵獁⁰牐⁯汁⁡敌⁵汇㱵牢ਾ††††††㌱〰††††††††㌱㔰††††††††㌱〱戼㹲䜊畬䄠慬倠潲䄠慬嘠污䄠灳倠潲嘠污䜠湬䜠畬䰠獹䰠略䜠畬䰠獹倠敨吠牨戼㹲 †††ㄠㄳ‵†††††††ㄠ㈳‰†††††††ㄠ㈳㰵牢ਾ汁⁡敓⁲祔⁲汇⁹敌⁵汇⁹敌⁵獁⁰敓⁲慖⁬汉⁥桐⁥獁⁮敍⁴獁⁰汇㱹牢ਾ††㌱〳††††††††㌱㔳††††††††㌱〴戼㹲吊牨䤠敬䜠畬䰠略䄠杲䰠略倠潲匠牥䜠祬䜠畬嘠污䤠敬䰠獹䰠獹䄠湳䰠略戼㹲ㄊ㐳‵†††††††ㄠ㔳‰†††††††ㄠ㔳‵†††††††ㄠ㘳㰰牢ਾ敓⁲獁⁰桐⁥汉⁥汁㱡牢ਾ††††††††㌱㔶戼㹲☊瑬㈻〱朦㭴㌠㌸戼㹲☊瑬㈻ㄱ朦㭴ㄠ㈱㰶牢ਾ氦㭴ㄲ☲瑧※剐㱔牢ਾ氦㭴ㄲ☳瑧※湕湫睯㱮牢ਾ氦㭴㈲☰瑧㰻牢ਾ氦㭴㈲☳瑧※䕎⁗ㄳ戼㹲☊瑬㈻ㄲ朦㭴嘠剁䅉呎戼㹲☊瑬㈻㈲朦㭴⠠⤱⸮⠮⤱戼㹲☊瑬㈻㌲朦㭴堠慡㴠䴠瑥楨湯湩⁥牯渠瑯楨杮戼㹲☊瑬㈻ㄲ朦㭴嘠剁䅉呎戼㹲☊瑬㈻㈲朦㭴⠠㔵⤷⸮⠮㔵⤷戼㹲☊瑬㈻㌲朦㭴堠慡㴠䜠祬楣敮漠⁲潮桴湩㱧牢ਾ氦㭴㈲☱瑧※䅖䥒乁㱔牢ਾ氦㭴㈲☲瑧※㔨㠵⸩⸮㔨㠵㰩牢ਾ氦㭴㈲☳瑧※慘⁡‽牐汯湩⁥牯渠瑯楨杮戼㹲☊瑬㐻〰朦㭴㌠㌸戼㹲堊慡䄠灳䤠敬䄠灳匠牥䰠略䰠略䰠獹䜠湬䰠略吠特䰠獹䰠略倠潲䰠略匠牥戼㹲ㄊ†††††††㔠†††††††††〱†††††††††㔱戼㹲䜊湬䄠杲䠠獩嘠污䜠畬匠牥䄠灳䜠祬䰠略䤠敬倠敨䄠灳倠潲䄠慬䜠湬䤠敬戼㹲 †††††㈠‰††††††††㈠‵††††††††㌠㰰牢ਾ桔⁲敓⁲牁⁧桔⁲汁⁡牁⁧汇⁹慖⁬汁⁡慖⁬牐⁯楈⁳汇⁹獁⁮楈⁳祔㱲牢ਾ††††㔳†††††††††〴†††††††††㔴戼㹲䠊獩倠敨䤠敬倠潲吠特䜠畬䜠湬䴠瑥匠牥䜠畬䰠略䜠畬䰠獹䄠杲䤠敬䄠慬戼㹲 †㔠‰††††††††㔠‵††††††††㘠㰰牢ਾ牁⁧汉⁥汉⁥牐⁯敌⁵牁⁧祔⁲牁⁧敓⁲獁⁮楈⁳牔⁰慖⁬牐⁯獁⁰敓㱲牢ਾ㔶†††††††††〷†††††††††㔷†††††††††〸戼㹲䄊杲倠潲䜠畬䜠畬倠潲匠牥倠潲䜠湬倠潲吠牨倠潲䜠畬倠潲匠牥倠潲匠牥戼㹲 †††††††㠠‵††††††††㤠‰††††††††㤠㰵牢ਾ牐⁯汇⁮牐⁯汁⁡牐⁯獁⁮牐⁯汇⁮牐⁯汁⁡牐⁯敓⁲獁⁮牐⁯汉⁥獁㱰牢ਾ††††††〱‰††††††††〱‵††††††††ㄱ㰰牢ਾ汇⁵祌⁳敌⁵慖⁬祌⁳汇⁵汁⁡慖⁬牁⁧祌⁳慖⁬汇⁹獁⁰汇⁹祔⁲慖㱬牢ਾ††††ㄱ‵††††††††㈱‰††††††††㈱㰵牢ਾ桐⁥汇⁵汇⁵獁⁮汇⁹慖⁬敓⁲牁⁧祔⁲汉⁥牐⁯汁⁡祌⁳獁⁮敌⁵敓㱲牢ਾ††㌱‰††††††††㌱‵††††††††㐱㰰牢ਾ汁⁡汇⁵桔⁲汁⁡汁⁡汇⁹汉⁥獁⁰敓⁲祌⁳敌⁵汁⁡祌⁳汇⁮汇⁵敓㱲牢ਾ㐱‵††††††††㔱‰††††††††㔱‵††††††††㘱㰰牢ਾ敌⁵敓⁲楈⁳祌⁳敌⁵汇⁹汁⁡祌⁳祌⁳桔⁲獁⁰敌⁵牐⁯敓⁲敓⁲獁㱰牢ਾ††††††††㘱‵††††††††㜱‰††††††††㜱㰵牢ਾ牁⁧汇⁵桐⁥祔⁲獁⁮祌⁳汁⁡祔⁲獁⁰敌⁵敌⁵汁⁡牁⁧汉⁥楈⁳汇㱮牢ਾ††††††㠱‰††††††††㠱‵††††††††㤱㰰牢ਾ獁⁰敌⁵敌⁵獁⁰獁⁮祌⁳汇⁹牁⁧汇⁮慖⁬獁⁰桐⁥汇⁵汁⁡敌⁵獁㱰牢ਾ††††㤱‵††††††††〲‰††††††††〲㰵牢ਾ獁⁮敌⁵敌⁵汇⁵牁⁧敌⁵祌⁳獁⁰慖⁬敓⁲敓⁲獁⁰祌⁳慖⁬祌⁳敌㱵牢ਾ††ㄲ‰††††††††ㄲ‵††††††††㈲㰰牢ਾ慖⁬獁⁰獁⁰汉⁥敌⁵汁⁡桐⁥敌⁵汁⁡牐⁯汉⁥牁⁧楈⁳牐⁯汇⁵牁㱧牢ਾ㈲‵††††††††㌲‰††††††††㌲‵††††††††㐲㰰牢ਾ敌⁵汇⁹祌⁳牐⁯獁⁮汁⁡汇⁮汉⁥桔⁲祔⁲桔⁲獁⁰獁⁰汇⁵汉⁥汇㱮牢ਾ††††††††㐲‵††††††††㔲‰††††††††㔲㰵牢ਾ慖⁬汁⁡祌⁳敌⁵汁⁡汇⁹祌⁳祔⁲桔⁲桔⁲汇⁵獁⁰汇⁹祔⁲汉⁥桐㱥牢ਾ††††††㘲‰††††††††㘲‵††††††††㜲㰰牢ਾ獁⁰牐⁯牁⁧獁⁰汉⁥桔⁲敓⁲獁⁰汇⁵汇⁹獁⁰汁⁡祔⁲慖⁬桔⁲牐㱯牢ਾ††††㜲‵††††††††㠲‰††††††††㠲㰵牢ਾ楈⁳敍⁴桔⁲楈⁳敓⁲楈⁳牔⁰汉⁥祌⁳祌⁳獁⁰敓⁲敌⁵敓⁲汇⁵汁㱡牢ਾ††㤲‰††††††††㤲‵††††††††〳㰰牢ਾ汇⁵牁⁧汁⁡汁⁡汁⁡汇⁮汁⁡祔⁲汁⁡祌⁳汇⁵祌⁳汇⁹敌⁵桔⁲牐㱯牢ਾ〳‵††††††††ㄳ‰††††††††ㄳ‵††††††††㈳㰰牢ਾ牐⁯敓⁲桔⁲獁⁰楈⁳汇⁮獁⁰敓⁲汇⁹獁⁮桔⁲汇⁵汁⁡祌⁳汇⁹汁㱡牢ਾ††††††††㈳‵††††††††㌳‰††††††††㌳㰵牢ਾ汇⁵汁⁡汉⁥祔⁲獁⁮牁⁧慖⁬祌⁳汁⁡汁⁡祌⁳祌⁳慖⁬牐⁯敌⁵獁㱰牢ਾ††††††㐳‰††††††††㐳‵††††††††㔳㰰牢ਾ牁⁧敍⁴牐⁯祔⁲獁⁮敌⁵汇⁮祔⁲桔⁲慖⁬汇⁵慖⁬祌⁳獁⁮汇⁹敓㱲牢ਾ††††㔳‵††††††††㘳‰††††††††㘳㰵牢ਾ敌⁵汉⁥汉⁥牐⁯楈⁳祔⁲獁⁰楈⁳祔⁲楈⁳獁⁮汉⁥祌⁳桐⁥汇⁵牔㱰牢ਾ††㜳‰††††††††㜳‵††††††††㠳㰰牢ਾ桐⁥獁⁰汇⁵汇⁹敌⁵祔⁲汇⁵汁⁡牐⁯祌⁳汇⁹祔⁲桔⁲敌⁵汇⁵獁㱰牢ਾ㠳‵††††††††㤳‰††††††††㤳‵††††††††〴㰰牢ਾ敌⁵敌⁵汁⁡桔⁲慖⁬祌⁳祔⁲祔⁲慖⁬汇⁵楈⁳牐⁯獁⁮汇⁵牁⁧牐㱯牢ਾ††††††††〴‵††††††††ㄴ‰††††††††ㄴ㰵牢ਾ楈⁳敓⁲獁⁰獁⁮汇⁹桐⁥汇⁹獁⁮汁⁡敓⁲獁⁰楈⁳慖⁬汇⁮牁⁧獁㱮牢ਾ††††††㈴‰††††††††㈴‵††††††††㌴㰰牢ਾ祌⁳獁⁮汇⁹汇⁮汁⁡獁⁰桔⁲獁⁮汇⁮桔⁲汇⁵祌⁳牐⁯敓⁲汇⁵汇㱵牢ਾ††††㌴‵††††††††㐴‰††††††††㐴㰵牢ਾ祌⁳牐⁯汇⁮桔⁲汇⁵祌⁳牐⁯汇⁵汇⁵汇⁵桔⁲牐⁯牁⁧汇⁵汇⁵祌㱳牢ਾ††㔴‰††††††††㔴‵††††††††㘴㰰牢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祬䄠湳䤠敬䄠慬䄠湳倠敨䜠畬戼㹲 †㔠〳††††††††㔠㔳††††††††㔠〴戼㹲䰊略䄠灳䜠湬倠潲吠特䰠略倠潲䜠祬䜠湬吠牨倠敨䰠獹吠特吠牨䤠敬䄠慬戼㹲㔊㔴††††††††㔠〵††††††††㔠㔵††††††††㔠〶戼㹲匊牥䰠獹䄠灳吠特倠潲䜠畬嘠污匠牥吠特䄠灳䜠祬吠牨倠敨吠牨嘠污倠潲戼㹲 †††††††㔠㔶††††††††㔠〷††††††††㔠㔷戼㹲吊牨匠牥䰠略䄠慬吠特䰠獹䴠瑥䄠慬匠牥䜠湬吠牨䤠敬倠敨吠特倠潲倠敨戼㹲 †††††㔠〸††††††††㔠㔸††††††††㔠〹戼㹲䠊獩䄠慬䜠祬䄠灳吠牨吠特䰠略䄠杲嘠污䄠湳倠潲䜠湬倠敨䄠慬嘠污倠潲戼㹲 †††㔠㔹††††††††㘠〰††††††††㘠㔰戼㹲䰊獹䜠祬吠牨䄠灳䄠慬䰠略嘠污䄠杲嘠污倠敨䄠灳䜠畬倠敨䠠獩䜠祬䄠湳戼㹲 †㘠〱††††††††㘠㔱††††††††㘠〲戼㹲䄊慬吠特䰠略䜠畬䄠湳䄠湳吠特䰠獹嘠污䜠祬䜠畬䤠敬䰠獹䰠略倠潲䤠敬戼㹲㘊㔲††††††††㘠〳††††††††㘠㔳††††††††㘠〴戼㹲倊潲䰠獹䰠略䄠湳䜠湬䜠祬吠牨吠牨䄠杲吠牨䄠慬䜠祬䄠湳䰠獹䤠敬倠潲戼㹲 †††††††㘠㔴††††††††㘠〵††††††††㘠㔵戼㹲嘊污吠牨倠敨䴠瑥䄠慬䄠湳䄠慬吠特䰠略䄠灳䄠湳䜠湬匠牥吠牨吠特䤠敬戼㹲 †††††㘠〶††††††††㘠㔶††††††††㘠〷戼㹲嘊污䜠畬嘠污倠潲䤠敬䰠略䜠畬䰠獹䜠畬䄠湳䜠湬吠牨䄠灳䰠獹倠潲匠牥戼㹲 †††㘠㔷††††††††㘠〸††††††††㘠㔸戼㹲䤊敬䰠略倠潲䜠湬倠敨䰠獹䄠杲䄠湳䰠獹䄠慬䜠湬䜠畬䄠湳匠牥䰠獹䰠略戼㹲 †㘠〹††††††††㘠㔹††††††††㜠〰戼㹲䄊灳䜠畬䰠獹嘠污䜠畬䜠畬倠潲䰠獹吠牨匠牥䜠畬䰠獹嘠污䜠畬䰠獹䜠畬戼㹲㜊㔰††††††††㜠〱††††††††㜠㔱††††††††㜠〲戼㹲䰊獹䰠略匠牥䜠畬吠牨䜠祬䄠湳匠牥吠牨匠牥䄠湳匠牥吠牨䰠略䜠畬䜠畬戼㹲 †††††††㜠㔲††††††††㜠〳††††††††㜠㔳戼㹲嘊污倠潲吠牨嘠污䄠灳倠潲嘠污䜠湬䜠畬䰠獹嘠污䄠慬䰠獹倠敨䄠慬䜠畬戼㹲 †††††㜠〴††††††††㜠㔴††††††††㜠〵戼㹲匊牥吠特䜠祬䴠瑥䰠獹䰠略䜠畬䄠湳嘠污䰠略倠敨䄠湳䴠瑥䄠灳䜠祬吠牨戼㹲 †††㜠㔵††††††††㜠〶††††††††㜠㔶戼㹲䤊敬䜠畬䰠略吠特䰠略倠潲匠牥䜠祬䜠畬嘠污䤠敬䰠獹䰠獹䄠湳䴠瑥䄠慬戼㹲 †㜠〷††††††††㜠㔷††††††††㜠〸戼㹲䄊灳倠敨吠牨䜠祬䜠畬䄠慬倠潲䜠湬䜠祬䄠湳䜠祬䜠畬䄠湳䰠獹倠潲匠牥戼㹲㜊㔸††††††††㜠〹††††††††㜠㔹††††††††㠠〰戼㹲䜊畬䄠湳䜠祬䰠獹嘠污匠牥吠牨䜠祬吠牨嘠污䜠畬䄠湳䜠湬倠潲吠牨䜠畬戼㹲 †††††††㠠㔰††††††††㠠〱††††††††㠠㔱戼㹲䄊湳䰠獹倠潲䄠慬䄠灳匠牥䰠略倠潲䜠畬䄠慬倠潲䄠湳䜠畬䰠獹倠潲嘠污戼㹲 †††††㠠〲††††††††㠠㔲††††††††㠠〳戼㹲䰊獹倠潲䜠畬䄠湳匠牥吠牨䄠灳䄠湳䜠祬䴠瑥䰠略䄠湳倠潲䜠畬䜠祬䄠湳戼㹲 †††㠠㔳††††††††㠠〴††††††††㠠㔴戼㹲嘊污䜠祬匠牥䄠灳倠潲䴠瑥䰠略䄠灳倠潲䄠慬䰠略䜠畬䜠畬䄠慬倠潲䄠慬戼㹲 †㠠〵††††††††㠠㔵††††††††㠠〶戼㹲嘊污䄠灳倠潲嘠污䜠湬䜠畬䰠獹䰠略䜠畬䰠獹倠敨吠牨䄠慬匠牥吠特䜠祬戼㹲㠊㔶††††††††㠠〷††††††††㠠㔷††††††††㠠〸戼㹲䰊略䜠祬䰠略䄠灳匠牥嘠污䤠敬倠敨䄠湳䴠瑥䄠灳䜠祬吠牨䤠敬䜠畬䰠略戼㹲 †††††††㠠㔸††††††††㠠〹††††††††㠠㔹戼㹲䄊杲䰠略倠潲匠牥䜠祬䜠畬嘠污䤠敬䰠獹䰠獹䄠湳䰠略匠牥䄠灳倠敨䤠敬戼㹲 †††††㤠〰††††††††㤠㔰††††††††㤠〱戼㹲䄊慬戼㹲邀鯥ꊝ臣ꆰ跥ꪁꫨ躘胣㲑牢ਾ胣뎛볯醀†偓㐶㰠⁵浸湬㩳灪∽瑨灴⼺眯睷樮潰朮⹯灪㸢噂ⵈ㰳甯몁볤邭臣䒮䅎藩鞈볯超裥ꪕ迥㪷볯ꞁ臣讂胣㲂牢ਾ胣뎛볯醀†菣꾂菣ꪂ菣覃㜱㜷胣㒜㤸ꮁ껥ꢅ臣厪㙐‴甼砠汭獮樺㵰栢瑴㩰⼯睷⹷灪⹯潧樮≰䈾䡖㌭⼼㹵臩鶼귥銂郥覜臣讂乄超裥鮼藩鞈闧랏銼臣芁苣芀戼㹲邀鯥鎼胣ₑ匠㙐‴甼砠汭獮樺㵰栢瑴㩰⼯睷⹷灪⹯潧樮≰䈾䡖ㄭ㰱甯몁볤邭臣䒮䅎藩鞈볯超裥ꪕ迥㪷볯ꞁ臣讂胣㲂牢ਾ胣뎛볯醀†菣꾂菣ꪂ菣覃㔴胣㊜㘵ꮁ껥ꢅ臣厪㙐‴甼砠汭獮樺㵰栢瑴㩰⼯睷⹷灪⹯潧樮≰䈾䡖ㄭ㰱甯몁볤邭苣ꮐ鳦馁苣䒋䅎藩鞈볯超裥ꪕ迥㪷볯ꞁ臣讂胣㲂牢ਾ胣뎛볯醀†菣꾂菣ꪂ菣覃ㄱ鲀㘲〳臣貮藥ꪁ偓㐶㰠⁵浸湬㩳灪∽瑨灴⼺眯睷樮潰朮⹯灪㸢噂ⵈㄱ㈭⼼㹵臩鶼귥銂郥覜臣讂乄超裥鮼藩鞈闧랏閼臣芁苣芀戼㹲邀鯥隼胣ₑ匠㙐‴噂ⵈ鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷볯ꞁ臣讂胣㲂牢ਾ胣뎛볯醀†偓㐶䈠䡖ㄭ鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷볯ꞁ臣讂胣㲂牢ਾ胣뎛볯醀†偓㐶䈠䡖ㄭⴱ鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷볯ꞁ臣讂胣㲂牢ਾ胣뎛볯醀†偓㌶䈠䡖㌭臩鶼귥꺁乄超裥鮼藩鞈闧랏馼臣芁苣芀戼㹲邀鯥醼볯醀†偓㌶䈠䡖㌭菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏ㄺꞁ臣讂胣㲂牢ਾ胣뎛볯醼胣ₑ㐠㑄㤮菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏ㄺꞁ臣讂胣㲂牢ਾ胣뎛볯銼胣ₑ㜠ㅇ⸱鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷㈱臣芁苣芀戼㹲邀鯥醼볯醀†䜷ㄱ㤮菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏ㄺꞁ臣讂胣㲂牢ਾ胣뎛볯钼胣ₑ㐠㍄㐮菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏ㄺꞁ臣讂胣㲂牢ਾ胣뎛볯閼胣ₑ㠠㍅ㄮ菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏ㄺꞁ臣讂胣㲂牢ਾ胣뎛볯隼胣ₑㄠ㉇㈮菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏ㄺꞁ臣讂胣㲂牢ਾ胣뎛볯鞼胣ₑㄠ䌰㈱㜮菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏ㄺꞁ臣讂胣㲂牢ਾ胣뎛볯颼胣ₑㄠ䘴⸶鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷㠱臣芁苣芀戼㹲邀鯥醼볯醀†ㅂ䐲⸸鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷㤱臣芁苣芀戼㹲邀鯥銼볯醀†䔷⸴鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷〲臣芁苣芀戼㹲邀鯥銼볯醀†〱㝄㔮菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏㈺ꞁ臣讂胣㲂牢ਾ胣뎛볯銼胣ₑㄠ䜰⸹鶃菣骃菣膃菣膀〱㉁㈮菣ꪃ菣鞃菣覃迥뎁ㅂ䈱⸸鶃菣骃菣膃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷㈲臣芁苣芀戼㹲邀鯥銼볯醀†ㄱ㡂㐮菣ꪃ菣鞃菣覃臣ꊂ菣躃藩超裥鮼藩鞈闧랏㈺ꞁ臣讂胣㲂牢ਾ胣뎛볯钼胣ₑ䴠扡䠠ㄱⵂㄱ㡂꣦蒚苣钃菣벃菣꺁苣龃菣뢅藩鞈볯超裥ꪕ迥㪷㘱ꞁ臣讂胣㲂牢ਾ胣뎛볯閼胣ₑ䈠䡖㌭臩鶼귥ꚸ臣ꮁ臣꺁藥랕迥뎁룤貮藥鶃菣骃菣膃菣꺁臩鶼귥超裥뎂菣螃苣뎃苣꺁뷤꺽臣芦闧蒚臣겪飦뎛臣芁苣膀乄궖觧鎖臣ꊖ뿤貁鯧銺臣몤臣貂苣芀戼㹲邀鯥銼볯醀†甼砠汭獮樺㵰栢瑴㩰⼯睷⹷灪⹯潧樮≰䈾䡖ㄭ㰱甯몁볤邭룤뎁臣鶁臣ꢅ闩誏臣趸껥ꢅ菣ꪃ菣鞃菣覃臣몁볤邭藩鞈苣벃菣ꎂ菣낂臣趽뷧꺁ꛦꖕ髧ꪁꫨ躘鯥ꞁ臣誂胣䒁䅎雦螉雩꺁雩芿臣뢛뫤ꮁꓧ閁苣讂胣㲂牢ਾ胣뎛볯鞼胣ₑ㰠⁵浸湬㩳灪∽瑨灴⼺眯睷樮潰朮⹯灪㸢噂ⵈㄱ㈭⼼㹵臩鶼귥ꚸ臣ꮁ臣꺁藥랕迥뎁룤貮藥鶃菣骃菣膃菣꺁臩鶼귥超裥뎂菣螃苣뎃苣꺁뷤꺽臣芦闧蒚臣겪飦뎛臣芁苣膀乄궖觧鎖臣ꊖ뿤貁鯧銺臣몤臣貂苣芀戼㹲邀鯥銼볯醀†噂ⵈ뾂菣醃苣ꪳ臣膀觧骮臣ꊃ菣꾂菣誃菣ꮃ諦鎽臣袂臣ꚁꫨ颭臣貂苣薆菩誏臣ꢡ鷩ꢂ菣袃菣鞃臣趽뷧꺁ꛦꖕ髧ꪁꫨ躘鯥ꞁ臣讂胣㲂牢ਾ胣뎛볯馼胣ₑ䈠䡖ㄭⴱ뾂菣醃苣ꪳ臣膀觧骮臣ꊃ菣꾂菣誃菣ꮃ諦鎽臣袂臣ꚁꫨ颭臣貂苣鶿귨ꢡ鷩ꢂ菣袃菣鞃臣趽뷧꺁ꛦꖕ髧ꪁꫨ躘鯥ꞁ臣讂胣㲂牢ਾ胣뎛볯邼胣ₑ鞃菣릂菣覃啰噒㈲䠮卉臣麃菣鞃臣芁苣膀態㱮畳⁰浸湬㩳灪∽瑨灴⼺眯睷樮潰朮⹯灪㸢㱒猯灵膀苣誃菣ꒂ苣뎃諦鞊胦뎂菣螃苣뎃苣颠鿥鮼䥣㔸膀菣꾂菣ꪃ苣閃苣벃苣캸₻䥣㔸ꦸ뫥龄迥Ꞁ菣鞃菣莃苣벃臩鶼귥鮼慬扭慤瀠膀菣꾂菣ꪃ苣閃苣벃苣캸ꊻ蛥鞃菣ꊃ菣뾂菣鮼楈⵳慴膀ⴶ菣릂菣뢂菣뎂菣螃苣뎃苣颠鿥鮼苣벃菣趃菣뾂菣膀ㅔ믨馆苣벃菣趃菣뾂菣鮼牯膀ꓨ붣臣掮汯ㅅ뗨邺胣㲂牢ਾ胣뎛볯醼胣ₑ䈠䡖㌭袼灳㐶볯ꢁ噂ⵈ袼灓㌶볯뾂菣醃苣ꪳ臣ꊂ菣躃藩超裥꺁胣䶁捡敖瑣牯藩鞈꟨邞苣閃菣Ꚃ苣ꊂ볯邃菣뢂菣뎃⸶⸵覼臣鞃菣낂菣ꂃ汃獵慴⁬銂铧蒁臣钯볨銂ꓧ鞁臣誁苣膀藩鞈臣许臣뎂菣뮂菣떂苣ꦃ苣뎃臣芁苣膀꣨랏胢誼胢誏臣鲀볯鶀臣膀臣貂臣貂郥肸迥뎁郥颧臣ꊂ菣躃藩议鿥銂ꓧ馁胣㲂牢ਾ胣뎛볯銼胣ₑ䈠䡖㌭胣䊁䡖ㄭ誏臣䊳䡖ㄭⴱ뾂菣醃苣ꪳ臣ꊂ菣躃藩超裥꺁胣䶁捡敖瑣牯藩鞈꟨邞苣閃菣Ꚃ苣ꊂ볯邃菣뢂菣뎃⸶⸵覼臣鞃菣낂菣ꂃ汃獵慴⁬銂铧蒁臣钯볨銂ꓧ鞁臣誁苣膀藩鞈臣许臣꾁苣뎃苣뎃苣릂菣ꒂ菣貁臣誂胣風迥鲀볯鶀迥뎁胢躼胢꾁胣鶁苣麁苣貐룤誏臣貐꟦꺁苣龃菣뢅껦몟苣몤臣芀戼㹲邀鯥鎼볯醀†甼砠汭獮樺㵰栢瑴㩰⼯睷⹷灪⹯潧樮≰举坅㌴⼼㹵臩鶼귥꺁乄超裥袼藩鞈闧랏㈺㜵볯ꞁ臣讂胣㲂牢ਾ胣뎛볯钼胣ₑ丠坅㌴菣ꪃ菣鞃菣覃臣ꢎ껥䒚䅎藩鞈볯超裥ꪕ迥㪷㔲覼臣芁苣芀⼼䥄㹖㰊䥄㹖䐼噉邀鯥醼胣㲑䐯噉ਾ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㜴⼼㹡⼼䥄㹖 †††ਠ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㠴⼼㹡⼼䥄㹖 †††㰠䥄㹖胣뎛볯醀⼼䥄㹖 †††††㰠䥄㹖愼栠敲㵦〢〰〵ㄵ㔹⸹楴≦〾〰〵ㄵ㔹⸹楴쉦゠〰㐰㰹愯㰾䐯噉ਾ†††† †††††㰠䥄㹖愼栠敲㵦〢〰〵ㄵ㔹⸹楴≦〾〰〵ㄵ㔹⸹楴쉦゠〰㔰㰰愯㰾䐯噉ਾ†††† †††††㰠䥄㹖愼栠敲㵦〢〰〵ㄵ㔹⸹楴≦〾〰〵ㄵ㔹⸹楴쉦゠〰㔰㰱愯㰾䐯噉ਾ††††䐼噉邀鯥鎼胣㲑䐯噉ਾ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㈵⼼㹡⼼䥄㹖 †††ਠ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㌵⼼㹡⼼䥄㹖 †††㰠䥄㹖胣뎛볯醀⼼䥄㹖 †††††㰠䥄㹖愼栠敲㵦〢〰〵ㄵ㔹⸹楴≦〾〰〵ㄵ㔹⸹楴쉦゠〰㔰㰴愯㰾䐯噉ਾ†††† †††††㰠䥄㹖愼栠敲㵦〢〰〵ㄵ㔹⸹楴≦〾〰〵ㄵ㔹⸹楴쉦゠〰㔰㰵愯㰾䐯噉ਾ††††䐼噉邀鯥閼胣㲑䐯噉ਾ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㘵⼼㹡⼼䥄㹖 †††ਠ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㜵⼼㹡⼼䥄㹖 †††㰠䥄㹖胣뎛볯醀⼼䥄㹖 †††††㰠䥄㹖愼栠敲㵦〢〰〵ㄵ㔹⸹楴≦〾〰〵ㄵ㔹⸹楴쉦゠〰㔰㰸愯㰾䐯噉ਾ††††䐼噉邀鯥鞼胣㲑䐯噉ਾ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㤵⼼㹡⼼䥄㹖 †††ਠ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰〶⼼㹡⼼䥄㹖 †††㰠䥄㹖胣뎛볯醀⼼䥄㹖 †††††㰠䥄㹖愼栠敲㵦〢〰〵ㄵ㔹⸹楴≦〾〰〵ㄵ㔹⸹楴쉦゠〰㘰㰱愯㰾䐯噉ਾ††††䐼噉邀鯥馼胣㲑䐯噉ਾ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㈶⼼㹡⼼䥄㹖 †††ਠ††††††䐼噉㰾⁡牨晥∽〰㔰㔰㤱㤵琮晩㸢〰㔰㔰㤱㤵琮晩ꃂ〰〰㌶⼼㹡⼼䥄㹖 †††㰠䥄㹖胣뎛볯邼胣㲑䐯噉ਾ††††††䐼噉㰾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