【手続補正書】
【提出日】平成22年8月18日(2010.8.18)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０２２６
【補正方法】変更
【補正の内容】
【０２２６】
［参考文献］
Archer, D., Jeenes, D. and Mackenzie, D. ( 1994). Strategies for improving heterologous protein production from filamentous fungi. Antonie Van Leeuwenhoek 65, 245-50.
Gasser, B., Maurer, M., Gach, J., Kunert, R. and Mattanovich, D. (2006). Engineering of Pichia pastoris for improved production of antibody fragments. Biotechnol Bioeng 94, 353-61 .
Gething, M. and Sambrook, J. (1992). Protein folding in the cell. Nature 355, 33-45.
Hohenblum, H., et al. (2003) Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J Biotechnol 102, 281 -290
Kurtzman CP. (2005). Description of Komagataella phaffiee sp. nov. and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella. lnt J Syst Evol Microbiol 55, 973-976.
Lang, C. and Looman, A. ( 1995). Efficient expression and secretion of Aspergillus niger RH5344 polygalacturonase in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 44, 147-56.
Macauley-Patrick, S., Fazenda, M. L., McNeil, B. and Harvey, L. M. (2005). Heterologous protein production using the Pichia pastoris expression system. Yeast 22, 249-70. 
Mattanovich, D., Gasser, B., Hohenblum, H. and Sauer, M. (2004). Stress in recombinant protein producing yeasts. J Biotechnol 1 13, 121 -35.
Mori, Ｋ．, Ogawa, N., Kawahara, T., Yanagi, H. and Yura, T. (2000). mRNA splicing-mediated C-terminal replacement of transcription factor Had p is required for efficient activation of the unfolded protein response. ProcNatl Acad Sci U S A 97, 4660-5.
Porro, D., Sauer, M., Branduardi, P. and Mattanovich, D. (2005). Recombinant protein production in yeasts. MoI Biotechnol 31 , 245-59.
Punt, P. J., van Biezen, N., Conesa, A., Albers, A., Mangnus, J. and van den Hondel, C. (2002). Filamentous fungi as cell factories for heterologous protein production. Trends Biotechnol 20, 200-6.
Sauer, M., Branduardi, P., Gasser, B., VaIIi, M., Maurer, M., Porro, D. and Mattanovich, D. (2004). Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation. Microb Cell Fact 3, 1 7. 
Shuster, J. (1991 ). Gene expression in yeast: protein secretion. Curr Opin Biotechnol 2, 685-90. 
Stryer, L. (1995). Biochemie. Spektrum der Wissenschaft Verlags GmbH. 
SEQUENCE LISTING
<110>  Polymun Scientific Immunbiologische Forschung GmbH
<120>  EXPRESSION SYSTEM
<130>  BEP-10289-WO
<140>  PCT/EP2008/003076
<141>  2008-04-17
<150>  EP 07008051.0
<151>  2007-04-20
<160>  147
<170>  PatentIn version 3.3
<210>  1
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer BFR2 FORW  PmlI
<400>  1
taaacacgtg agcatggaaa aatcactagc gg                                   32
<210>  2
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer BFR2 BACK SacII
<400>  2
tacaccgcgg tcaaccaaag atttggatat c                                    31
<210>  3
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer BMH2 FORW PmlI
<400>  3
taaccacgtg agcatgtccc aaactcgtga ag                                   32
<210>  4
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer BMH2 BACK SacII
<400>  4
tatgccgcgg ttatttggtt ggttcacctt g                                    31
<210>  5
<211>  34
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer COG6 FORW PmlI
<400>  5
taagcacgtg agcatggatt tcgttgtaga ctat                                 34
<210>  6
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer COG6 BACK SacII
<400>  6
taagccgcgg tcagtgatca atacctatca ac                                   32
<210>  7
<211>  33
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer COY1 FORW PmlI
<400>  7
tagtcacgtg agcatggata cgtcagtata ttc                                  33
<210>  8
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer COY1 BACK SacII
<400>  8
tacaccgcgg ctatcgattt atgccatgaa c                                    31
<210>  9
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer CUP5 FORW PmlI
<400>  9
tatccacgtg agcatgactg aattgtgtcc tg                                   32
<210>  10
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer CUP5 BACK SacII
<400>  10
tacaccgcgg ttaacagaca acatcttgag                                      30
<210>  11
<211>  42
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer ERO1 FORW SfiI
<400>  11
tataggccca gccggccacg atgagattaa gaaccgccat tg                        42
<210>  12
<211>  33
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer ERO1 BACK SacII
<400>  12
tgtcccgcgg ttattgtata tctagcttat agg                                  33
<210>  13
<211>  39
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer IMH1 FORW SfiI
<400>  13
tataggccca gccggccacg atgttcaaac agctgtcac                            39
<210>  14
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer IMH1 BACK SacII
<400>  14
tagaccgcgg ttacttcaga gacataacca g                                    31
<210>  15
<211>  42
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer KIN2 FORW SfiI
<400>  15
tcaaggccca gccggccacg atgcctaatc cgaatacagc ag                        42
<210>  16
<211>  38
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer KIN2 BACK SacII
<400>  16
tctgccgcgg ctataggttt aattctttta aaatatac                             38
<210>  17
<211>  34
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer KAR2 FORW PmlI
<400>  17
taagcacgtg acgatgtttt tcaacagact aagc                                 34
<210>  18
<211>  29
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer KAR2 BACK SacII
<400>  18
tatgccgcgg ctacaattcg tcgtgttcg                                       29
<210>  19
<211>  33
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer PDI1 FORW EcoRI
<400>  19
cgccgaattc acgatgaagt tttctgctgg tgc                                  33
<210>  20
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer PDI1 BACK XhoI
<400>  20
cctcctcgag ttacaattca tcgtgaatgg c                                    31
<210>  21
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer SEC31 FORW SfiI
<400>  21
tataggccca gccggccacg atggtcaaac ttgctgagtt                           40
<210>  22
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer SEC31 BACK SacII
<400>  22
tatgccgcgg ttaattcaaa gtcgcttcag c                                    31
<210>  23
<211>  35
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer SSA4 FORW PmlI
<400>  23
tatgcacgtg acgatgtcaa aagctgttgg tattg                                35
<210>  24
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer SSA4 BACK SacII
<400>  24
tatcccgcgg ctaatcaacc tcttcaaccg                                      30
<210>  25
<211>  35
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer SSO2 FORW PmlI
<400>  25
tacacacgtg acgatgagca acgctaatcc ttatg                                35
<210>  26
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer SSO2 BACK SacII
<400>  26
tatgccgcgg ttactttctt gtttccacaa cg                                   32
<210>  27
<211>  35
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer SSE1 FORW PmlI
<400>  27
tagacacgtg acgatgagta ctccatttgg tttag                                35
<210>  28
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer SSE1 BACK SacII
<400>  28
tatcccgcgg ttagtccatg tcaacatcac c                                    31
<210>  29
<211>  42
<212>  DNA
<213>  Artificial
<220>
<223>  Forward primer HAC1 FORW SfiI
<400>  29
gcaaggccca gccggccacg atggaaatga ctgattttga ac                        42
<210>  30
<211>  19
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer HAC BACK1
<400>  30
tggtcatcgt aatcacggc                                                  19
<210>  31
<211>  88
<212>  DNA
<213>  Artificial
<220>
<223>  Backward primer HAC BACK2 SacII
<400>  31
cctcccgcgg tcatgaagtg atgaagaaat cattcaattc aaatgaattc aaacctgact     60
gcgcttctgg attacgccaa ttgtcaag                                        88
<210>  32
<211>  822
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  32
atgtcccaaa ctcgtgaaga ttctgtttac ctagctaaat tagctgaaca agccgaacgt     60
tatgaagaaa tggtcgaaaa catgaaggcc gttgcttcat caggtcaaga gttatctgtc    120
gaagaacgga atctattgtc ggttgcttac aagaacgtca tcggtgctcg ccgtgcttca    180
tggagaatag tttcttcgat cgaacaaaaa gaagaatcaa aggagaaatc tgaacatcaa    240
gttgaattaa tccgttctta ccgttctaaa attgaaactg aattgaccaa aatctctgac    300
gacattttat ctgtgttaga ttctcattta atcccttctg ctactactgg tgagtctaaa    360
gtattttact ataagatgaa gggtgactac caccgttatt tagctgaatt ttccagcgga    420
gatgcaagag aaaaggcaac caactcctct ttggaggctt ataaaaccgc ttccgaaatc    480
gccacaactg aattgcctcc aactcaccca attcgtttag gtctagcttt gaatttctcc    540
gtcttctatt acgaaattca aaactctcct gataaggctt gccacttggc caaacaagcc    600
tttgatgatg ctattgctga gttagatact ttatctgaag aatcatacaa ggatagcact    660
ttgatcatgc aattattaag ggacaacttg accttatgga cctctgatat ttctgaatct    720
ggtcaagaag atcaacaaca acaacaacaa cagcaacagc aacagcaaca acagcaacaa    780
caagctccag ctgaacaaac tcaaggtgaa ccaaccaaat aa                       822
<210>  33
<211>  1605
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  33
atggaaaaat cactagcgga tcaaatttcc gatatcgcca ttaaaccggt caataaagac     60
ttcgatattg aagatgagga aaatgcatct ttatttcaac acaatgaaaa aaatggagaa    120
agtgatttaa gcgactatgg aaatagcaac acagaagaaa ccaagaaggc gcactatttg    180
gaggtggaaa agtctaagtt aagagcagaa aaaggtttag aactaaacga tccaaaatat    240
acaggtgtta aaggttcaag acaagcatta tatgaagaag tttccgagaa tgaggacgaa    300
gaagaagaag aagaagagga agaagaaaaa gaggaagatg ctctttcatt caggacagat    360
tctgaagatg aagaagtaga gattgatgaa gaagaatcag acgcggacgg cggtgaaacg    420
gaggaggctc aacagaaaag gcatgcacta tcgaaactaa ttcaacaaga gactaaacaa    480
gctattaaca aactgtctca atcagttcaa agagatgctt cgaagggtta ttccatttta    540
caacagacaa aattatttga caacatcatt gatttgagaa taaaactaca aaaagctgta    600
attgcagcaa ataagctccc attaactaca gagtcctggg aagaggctaa aatggatgat    660
tcagaggaaa caaagcgttt gctgaaggaa aacgaaaaac tgttcaataa tttattcaat    720
cggttgataa atttcagaat aaaattccaa cttggcgatc atatcactca aaatgaagag    780
gtggcgaagc ataaattgtc caaaaaaaga tctctcaaag agctttacca agaaactaat    840
agcttagact cagaactaaa agagtacagg actgccgtat taaacaagtg gtctaccaaa    900
gtttcttctg catcaggtaa cgctgcttta tcatctaaca aattcaaagc tatcaactta    960
cctgcagatg tacaagtcga aaaccaatta tccgatatgt cccgtttgat gaaaagaaca   1020
aagttgaaca ggagaaacat aacgcctttg tatttccaaa aagactgtgc taatggcagg   1080
ctaccagaat tgatttctcc cgttgtcaaa gatagtgttg atgacaatga gaattcggat   1140
gatgggcttg atatcccgaa aaactatgac ccaagaagaa aggataacaa tgccattgac   1200
attaccgaaa acccatatgt ttttgatgac gaagattttt accgtgtttt actaaacgat   1260
ttaattgaca aaaagatttc caacgctcac aattctgaaa gtgcagcaat tacaatcacc   1320
tcaactaatg ctcgttcgaa caacaagcta aagaagaata tcgatactaa ggcttccaag   1380
ggtaggaaat tgaactactc agttcaagat ccaattgcga attatgaagc ccccatcaca   1440
tccggataca aatggtcaga cgaccaaatc gatgaattct ttgcgggatt gttaggtcaa   1500
cgagtgaact ttaatgaaaa tgaggatgag gaacaacatg ccagaataga aaatgacgaa   1560
gaattagagg ctgttaaaaa cgatgatatc caaatctttg gttga                   1605
<210>  34
<211>  2520
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  34
atggatttcg ttgtagacta tcagacctac gcaatggcgg atactgccac gccagaatta     60
ccagaacctg agccaagact aaacttaacc tcagatgcac agtcacagcc caccggtaaa    120
ctagatctac agtttaagtt gcccgacctt caacgttatt ccaataataa tgcaactttg    180
ccagtagata atgatggtgc tggttcgaaa gacctacata agaaaatgac acattacgca    240
atgtcttcca ttgataaaat acagctttca aatccaagca aacaattagg gcaaaattcc    300
caggatgaaa aactatcgca gcaagaatct caaaatttca cgaattacga gccaaaaaac    360
cttgatttat caaaattagt atccccgtca agtggttcca acaaaaatac cacaaatttg    420
gttctttcga ataaactatc caagatattg aacaattaca cattgattaa ctatcaggcc    480
acagtccaac taagaaaatc cctaaaggtt ctagaagaga ataaagagag attgtccctt    540
gatgaacaaa agctcatgaa tcctgaatat gtaggtactt tggcaagaag agcattgagg    600
actgatttgg aatctcaact gctaaaggaa catattacgg tacttgagga attcaaacct    660
atcattagaa ggattaaacg attatcttct tccgtcgaaa aaatacaaag aacgagcgaa    720
aaattactaa gtaatgagac aaatgaggtt ccaacaaata acgtggtact tcaggaaata    780
gatcaatacc gtttaaaggc agagcagttg aagctgaaaa aaaaaatact gttatctata    840
agggataggt ttactttgaa tcaggtagag gacgatgtaa tcaccaatgg tactatagac    900
aacatctttt tcgaggtagt aaagaaagta atcaatatta aagatgaatc aagtttcttg    960
ctgacgcttc ctaatttgaa tgctggaaat gctttgataa tgggagttaa tgaaatttta   1020
gaaaagacaa acaaaaaaat cttcaattat ttgatcgatt ttttatatag ttttgaatcc   1080
tcttcaaatt tattaaatga ccatggtact actgaacaag aaagcttaaa catttttcgg   1140
aagagtctgg tcttcctgtc aagtgatcta gaattattta atgagttgtt gaaaagagtg   1200
accacactga gatccaagag tattctggat gagtttttgt ctcaattcga tatgaattca   1260
actacctcta aacccatcat attatcggca cacgatccaa ttaggtatat tggtgacgta   1320
ctagcgtccg ttcattccat catcgcaaat gaagctgatt tcgtgaagtc actatttgac   1380
tttcaggatg aagacttaaa agatacccca atttctatac ttcaacaaaa caagacattc   1440
ttgaaaggca tcgacaacaa attgctgaac gatatcatcc agtcgctatc caattcgtgt   1500
cgtattcgta tcgagcaaat cgtgaggttt gaagaaaatc cgatcatcaa tttcgagatt   1560
gtgaggctgc tgaaacttta cagagttatg ttcgagagaa agggaattca ggacgatagt   1620
tctattatta acaatttaaa gtcgttggaa gacatttcca aaaacagaat tattggatac   1680
tatgaagact atatgaagca aacagtcatg gcggaaacaa aaaattcttc agatgattta   1740
ctgccaccag agtggctatc agagtatatg aataaattgg tagagttatt tgaaatttat   1800
gaaaagacac atgctgccga agatgaggaa tcagaagata ataaattgct ctcatctaag   1860
aatttacaaa caattgtaga acaaccaata aaagatgttc tgttaaaaca attgcaaaca   1920
tcttttcctt tggcgaaaaa aaatgaaaaa gaaaaggcat cattgctaac tatagagata   1980
aactgtttcg atttaattaa atctagactt caaccttttg agggattgtt tgcacaagat   2040
gatgacagcc ggaaaatcac catctgggtt tgtgataaac tgaaggaata tactaagcaa   2100
atgctaactt tacaaataaa attcctattt gagaatacag gtttagacct ttacagcaat   2160
ttggtcaata tgatttttcc tgtggactca gtaaaggatg aattggatta tgatatgtac   2220
ttagccctga gggataattc attgatggaa ttagacatgg tcagaaaaaa tgtgcatgat   2280
aagttgaact attatctacc tcaggcgtta acagatgttc aaggtaattt actatttaaa   2340
ttaacgtcac caatgatagc tgatgaaata tgcgatgaat gtttcaagaa gttgtcgcta   2400
ttttataata tcttcaggaa actgttgatt catttgtatc cgaacaagaa ggatcaggta   2460
ttcgaaattt taaatttttc cactgatgaa tttgacatgt tgataggtat tgatcactga   2520
<210>  35
<211>  2039
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  35
atggatacgt cagtatattc tcatgcattg gatatttggg ccaaggcaga tttaacgaat     60
cttcaaagag aattggatgc tgatgttata gagattaagg ataaagaaac cctgtccttg    120
aattcaagaa agtcattagc cactgagact aaaaaattta aaaaactcga acctgaggaa    180
aaattgaaca atgtgaataa aataattaag cagtaccaac gtgaaattga taatttgaca    240
cagagatcaa aattctctga aaaggttctt tttgacgtat acgaaaagct ttcagaggct    300
cctgatccac agccgctact acaaagttcg ttggaaaaat tgggcaaaat tgatgactcg    360
aaggaactta aggaaaaaat aagctaccta gaagataagc tagccaaata tgcagattat    420
gagactttga aatcaaggtt actggaccta gagcaaagct ctgcaaaaac attggcaaaa    480
agactgactg cgaaaactca agaaatcaat tctacctggg aggaaaaagg aagaaattgg    540
aaagagagag aagcagatct attgaaacaa ttaacaaatg tacaggagca aaacaaggca    600
ctagaggcca aaatatctaa aaatatagat atagaaggta atggaaacga agatggtgac    660
caagaaaaca atcaaaaaga agtatctaca aggattgctg aatataatct agtaacacag    720
gagttggaaa ctacgcaggc tagaatatat cagttagaga aaagaaatga ggaactaagt    780
ggtgctcttg caaaggcaac tagtgaagca gaaaaagaaa ctgagttaca tgcaaaggaa    840
ctaaaactta accagctgga aagcgaaaat gcattgttga gtgcatccta tgagcaggaa    900
cggaaatcaa catcacatgc aataaatgag ttaaaagaac aattaaatag cgttgtggcg    960
gaatcggaat cttacaagtc ggagctagaa actgttagaa gaaaactaaa caattattct   1020
gattacaata agataaaaga agaactttct gcattgaaaa aaattgagtt tggggtaaac   1080
gaagatgatt ctgataatga cattcgctct gaagacaaga atgataatac tttcgaaagt   1140
tccttactat ctgcaaataa gaagctccag gctactttgg cggaataccg ctcaaaaagt   1200
acggctcaag aggaagaacg aaacgaattg aaaaaatctg tggaccaatt gaagcagcaa   1260
atagctactc tcaaagaagc aaatgaaaaa ttagagacgg acctagaaaa agtagagaac   1320
gtcagtcctc acttcaacga gactgcaagt atgatgtctg gtgtaacaag acaaatgaac   1380
aatcgtacgt cccataaaat gtccccaacg agttctatta ttggtattcc agaagatggg   1440
gaactttctg gaaaccaatc aaccatttta ccaatagtta ctaaacaaag agacagattt   1500
cgttcgagaa atatggatct ggaaaagcaa ctaagacaag gaaactcaga aaagggtaag   1560
cttaaactag aaatttcgaa gctaaaaggc gacaatacga agctttatga acggattagg   1620
tatctgcaat cctataataa taacaacgct cccgttaatc aaagtacaga gcgtattgac   1680
gtggaatccc aatactcaag ggtgtatgat gaatcgttgc atccaatggc aaattttaga   1740
cagaacgaat taaaccacta caaaaacaag aaattatcag ctttagagaa gttattttcc   1800
agttttgcaa aagtcatttt acaaaataaa atgacaagga tggtattcct cttttactgt   1860
atcggtttac acggactcgt attcatgatg agcatgtatg tgattaatat tagcggctac   1920
atgacacctg aggttggtat agtacaatcg gcaaagtctt cttcaaatct caacggagga   1980
cttgggggag cagaaaaagt agctgcaggc gttggttcag ttcatggcat aaatcgata    2039
<210>  36
<211>  483
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  36
atgactgaat tgtgtcctgt ctacgcccct ttctttggtg ccattggttg tgcctctgca     60
attatcttca cctcattagg tgctgcttac ggtactgcta agtctggtgt tggtatctgt    120
gccacttgtg tgttgagacc agacctatta ttcaagaaca ttgttcctgt tattatggct    180
ggtatcattg ccatttacgg tttagttgtt tccgttttgg tttgttattc gttgggtcaa    240
aagcaagctt tgtacaccgg tttcatccaa ttgggtgccg gtctatcagt cggtttgagt    300
ggtctagctg ctggtttcgc tattggtatt gtcggtgatg caggtgttag aggttcctct    360
caacaaccaa gattattcgt cggtatgatt ttgattttga tttttgctga agttttgggt    420
ctatacggtt tgattgttgc tttgttgttg aactccaggg ctactcaaga tgttgtctgt    480
taa                                                                  483
<210>  37
<211>  2736
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  37
atgttcaaac agctgtcaca aattggtaag aatcttaccg atgaattagc gaagggctta     60
gccgatgata tgagccctac cccgtcagaa caacaaatcg aagatgataa gagtggcttg    120
ccaaaagaaa tacaagctaa attaagaaaa tttgagaaat atgaacaaaa ataccctttg    180
ctactctccg catacaaaaa tgaaaaatta aagtcagaga agttagaggc tgttgaaaag    240
attttagcgg aaaatacacc catatctaat attgacgacg cagtggatac gttgccagct    300
tttttccagg atttaaacaa caaaaataac ctattgaatg atgagatcaa gagattaact    360
aagcagaact cggaaattcc agaaagcgcc tctagtgaaa ctctgaagga taaagaagag    420
gaatttttga aaaaagagca aaattataaa aatgacatag acgatctaaa aaaaaaaatg    480
gaagctttaa acatagaatt ggatactgta caaaaagaaa aaaatgatac tgtttcaggt    540
ttgagagaaa aaatagttgc actggaaaat atactaaagg aagaaaggga ggccaaaaaa    600
cagaaagaag aagtatctat atccgaactg aaggaagaat tggctataaa gaaccattct    660
ctcgaggaca gtcgaatgaa gataaccgaa ttggagcaaa atttgtcttc gaaaagtact    720
ataatggagg aaaagtcctc agagttggca gaactaaata ttactttaaa agagaaagag    780
cgcaagctga gtgaattgga aaaaaaaatg aaggagttac cgaaggcgat atctcatcaa    840
aatgtaggaa acaataacag aaggaaaaag aatagaaaca agggaaagaa aaataaggga    900
ggcataacta cgggtgatat cagtgaagag gaaacggtcg ataactcaat caatactgaa    960
gaatatgata agcttaaaga aaatttgcaa gaattacaag aaaaatataa agattgtgaa   1020
gattggaagc aaaagtatga agatatagaa gcagaactaa aagatgctaa agaattggaa   1080
aactcacagc tcgaaaaatc agcaaaggag ctggaaaccc ttaacaccga gttgatcgat   1140
accaagaagt cattgaaaga aaaaaattcg gagctagagg aggtgagaga tatgctgagg   1200
actgtaggca atgagcttgt ggacgcaaaa gatgagatta aagagtcttc gagtaaacaa   1260
aatgaagaag tgaaaaccgt taagctggag ctcgatgatt tacgccataa aaatgcaacg   1320
atgatcgagg cctacgaagc taaaaatact gagttgagaa gtaagataga gttattgagc   1380
aagaaagtag agcatctgaa gaatttatgt acagaaaagg agaaagagca gactacatcg   1440
cagaacaagg tagccaaatt aaatgaggag atatctcaac ttacctacga aaaatcaaac   1500
ataacaaagg agcttacttc tttaagaacc tcttataaac aaaaggagaa aactgtgagt   1560
tacttggagg aacaagttaa acaatttagt gagcaaaagg acgtggctga aaaatccaca   1620
gaacagctga gaaaagatca tgctaaaatt tctaacagat tagacttatt aaaaaaggaa   1680
aatgagacac tgcataatga tatcgcaaag aattctaatt cctacgagga gtatttgaaa   1740
gaaaatggta aattatcgga aagattgaat attttgcaag aaaaatacaa taccttgcaa   1800
aatgtaaaaa gtaattcgaa tgaacacata gattctatca aaagacaatg tgaggaacta   1860
aatgtcaagt tgaaggaatc tacaaaaaaa attttatctt tagaagatga actaaatgaa   1920
tatgctaata ttgttcaaga caaaaccaga gaagctaaca cattgagaag gttagtttcg   1980
gacagtcaga cagatgattc gagcaaacaa aaagagttgg agaataaatt ggcctattta   2040
acggatgaaa agaataaatt ggaagcagaa ttagacttac aaacatccag aaaggccact   2100
gaattacaag agtggaagca tacagtaact gagctgaaat cggaaataca cgctttaaag   2160
cttcgtgaag agggactaaa atcagaggtt gacgcattga aacatgttaa caatgacatc   2220
aaaaggaaga ctcaagccac ttcagatgat tccgatcagt tggaacagat cacatctaat   2280
ttaaaactct cattgtctaa ggctgatgaa aagaattttg agctacagtc tgccaatgag   2340
aaacttctga atttaaataa cgaacttaac aagaaatttg atcgattact aaaaaattat   2400
cgttcattgt cctctcaatt gaatgcttta aaggaaagac aatacagtga caagtcagga   2460
agagttagta ggtctggttc tatcggtact ctagctaacg cgaatattga ttcctcacca   2520
gcgaataact ctaatccaac taaattagag aagatacgat catcaagttc attggagtta   2580
gactctgaga aaaatgaaaa aattgcatat ataaaaaatg ttttgttggg atttttggag   2640
cacaaggaac aacggaacca attacttcct gtaatttcta tgttgttaca actggacagt   2700
actgatgaaa aaagactggt tatgtctctg aagtaa                             2736
<210>  38
<211>  3444
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  38
atgcctaatc cgaatacagc agattacttg gtgaatccaa atttcaggac cagtaagggc     60
ggatctttat cgccgacgcc agaagctttc aacgacacgc gagttgctgc accagccact    120
cttcgcatga tgggcaagca atctggacca agaaatgacc agcaacaagc accactgatg    180
cctcctgcag atatcaaaca gggcaaggaa caggcagctc agagacaaaa tgatgcatcg    240
aggcctaatg gcgccgtgga attaaggcaa tttcatagaa gatctttggg agattgggag    300
ttccttgaaa cggttggcgc aggctctatg ggtaaagtta aattggtcaa gcatcgtcaa    360
acaaaggaaa tttgtgtaat aaagattgtt aatagggctt ccaaggctta tctccataaa    420
cagcactctt taccttcccc aaagaatgag agtgagatat tagaaagaca aaagcggtta    480
gaaaaagaaa ttgcgaggga taaaaggact gttagggaag cctctttggg ccaaatcctt    540
taccatcctc atatctgtcg tttatttgaa atgtgcacta tgtcaaacca tttttatatg    600
ctttttgaat acgtttccgg tggacagctg ttagattata ttattcagca tggctcatta    660
aaggaacacc atgcgaggaa atttgccaga ggtatagcta gtgcgctgca atacttacat    720
gccaataata ttgttcatcg agatctgaaa attgagaata taatgatatc tagttcaggt    780
gaaattaaga tcattgattt tggtctttcc aacatttttg attataggaa acaattacat    840
acgttttgtg gttccttgta ctttgcagca ccagaactat taaaagcgca gccatacaca    900
ggacctgagg tagatatttg gtcgtttggt attgttcttt atgtcttggt ctgcggtaaa    960
gtaccatttg atgatgagaa ctcaagcatt ttacatgaaa aaataaaaaa aggtaaagta   1020
gactatcctt cacacttatc cattgaagtt atatctttat taaccaggat gattgttgtc   1080
gacccattaa gaagagcaac attaaagaat gtcgttgagc atccatggat gaacagagga   1140
tacgatttta aggctccatc atatgttcct aatcgtgttc cattaacccc tgaaatgata   1200
gatagccaag ttctgaagga aatgtatcgc ctagaattta ttgacgatat tgaagataca   1260
agaagatcat tgatccgatt agtaactgaa aaggaataca tccaactttc ccaagaatac   1320
tgggacaaat tatccaacgc caaggggttg agttcaagtt taaataataa ctacctaaat   1380
tcaacggcac aacaaacctt aatacaaaat catattacaa gtaatccatc gcaaagtggt   1440
tataatgaac cagatagtaa ttttgaagat cctactttag catatcatcc attactatca   1500
atatatcact tggtttcaga aatggttgca cggaaattag cgaagttgca aagaaggcaa   1560
gcattggccc tgcaagcgca agctcagcaa aggcaacaac agcaacaagt agcacttggc   1620
actaaggtcg ccttaaataa taactccccg gatattatga ccaaaatgag gagccctcag   1680
aaagaagtag tacctaatcc tggtattttt caagtgccgg caattggaac atcgggaacc   1740
tcaaacaaca ctaatacctc aaacaaacct ccactgcatg taatggttcc tcctaaacta   1800
acaataccgg aacaagcgca tacttctcca acatctagga agagttccga cattcatacg   1860
gaattaaatg gtgttttgaa atcaacacca gtccccgtgt ctggcgaata tcagcaacgt   1920
tctgcttcac ccgtagtagg tgaacatcag gaaaagaata caataggcgg catattcaga   1980
agaatatcac aaagtggaca atctcagcat cccacacggc aacaggaacc tcttccagaa   2040
agagaacctc caacatatat gtcaaaatca aatgaaattt ccatcaaagt accgaaaagc   2100
catagtcgta ctatatcaga ttatattcct agcgctagaa gatatccatc ttacgtgcca   2160
aattctgttg atgtaaaaca gaaacccgct aaaaacacta ccatagcacc tcctataagg   2220
tcagtatcac aaaagcaaaa cagtgatctt ccagctttac ctcagaacgc cgaactaatt   2280
gttcaaaaac aacggcaaaa actattacag gaaaatctcg acaaattaca aattaatgat   2340
aatgataaca acaatgtgaa cgctgtagtc gatggtatca ataatgataa tagtgaccat   2400
tatctctccg ttccgaaggg tcgtaagtta catcctagtg caagggctaa atcggtgggg   2460
catgctcgtc gtgaatcttt gaaatttact aggccgccta taccagcagc ccttccgcca   2520
tcagatatga caaacgataa cggctttttg ggagaggcaa acaaggagag atacaatcct   2580
gttagcagta acttttcgac cgttcctgaa gattctacca catacagtaa cgatactaac   2640
aatagactga cttcggtgta ttctcaggag cttactgaga agcaaatttt ggaggaagct   2700
tcaaaggcac cccccgggtc tatgccatca attgattatc caaagtcaat gtttttgaag   2760
ggttttttct ctgtacaaac aacctcctct aaaccattgc ctattgttcg tcacaatatc   2820
atatctgttt taacaagaat gaatattgat ttcaaagaag tgaaaggcgg gttcatatgt   2880
gtccaacaaa ggccatctat tgagactgca gctgtccctg ttataaccac tactggcgtg   2940
ggtttggatt ccggaaaggc gatggatctg caaaatagtt tagacagtca attatcatcc   3000
agttaccata gtacagcgtc ctcagcatca agaaatagtt cgataaaacg ccaaggttct   3060
tataagaggg gccagaataa tataccacta acacctttag cgaccaatac acatcaaaga   3120
aattcatcta tcccaatgtc tccaaactac ggaaaccaaa gtaatggtac atcaggggaa   3180
ctatcttcca tgtcattaga ttatgttcaa caacaggatg atattttaac aacatcaaga   3240
gcccaaaata taaataacgt aaatggtcaa acagagcaaa ccaatacttc tggtataaaa   3300
gaaaggcctc ctattaaatt tgagattcac attgtaaagg ttcgtatcgt cggcctagca   3360
ggtgtacatt tcaaaaaggt ttctggtaat acgtggctat ataaagaatt ggcatcgtat   3420
attttaaaag aattaaacct atag                                          3444
<210>  39
<211>  3822
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  39
atggtcaaac ttgctgagtt ttctcgaaca gccacgtttg cgtggtcaca tgataaaatt     60
ccattattgg tctctggtac cgtatctggt acggtggatg ctaatttctc cactgattca    120
tctctagaat tgtggtcatt gttggctgct gattcggaga agcctattgc ttccttgcaa    180
gtggattcca aattcaatga tttggattgg tctcataata acaagattat tgctggtgct    240
ctggataacg gtagtttgga attgtactcc accaatgaag caaacaacgc tatcaactcc    300
atggccagat ttagcaacca ttcttcctct gtgaagacgg taaagtttaa cgcaaagcaa    360
gacaacgttc ttgcttcggg tggtaacaac ggtgaaattt ttatttggga catgaataaa    420
tgcactgaat cgccctccaa ttatactcca ttgacaccgg gtcaatcgat gtcgtccgtt    480
gacgaggtca tttccctagc atggaaccaa tctttggccc atgtttttgc atctgccggg    540
tcgtctaatt tcgcatctat ttgggatttg aaggctaaga aggaagtcat tcatctaagt    600
tacacttcac ctaattcagg tatcaagcaa cagctgtccg ttgttgaatg gcacccaaaa    660
aactccacaa gagtggcaac ggctactggt agcgataatg atccatctat cctgatctgg    720
gatttaagaa acgccaacac accattgcag actttaaatc aaggccatca aaagggtatt    780
ttgtcattag attggtgtca tcaggacgaa catctattat tgtccagtgg tagagataat    840
accgttcttc tatggaaccc tgagtcagcc gaacaactgt cccaattccc agctcgtgga    900
aactggtgtt ttaagaccaa atttgcacca gaggctccag acctatttgc ttgtgcctcc    960
tttgataaca aaattgaggt acagactttg caaaatctca caaacacttt ggatgagcaa   1020
gaaaccgaaa ctaagcagca agaatctgaa acagattttt ggaataatgt ttcccgagag   1080
gaatcaaaag agaagccatc tgttttccat ttacaagccc caacttggta tggggaacca   1140
tctcccgcag ctcattgggc tttcggtggt aaattggttc aaattactcc agatggtaaa   1200
ggtgtatcta taacaaaccc aaaaatttca ggcttagaat caaacactac tttgagtgaa   1260
gcgttgaaaa ctaaggattt caaaccatta ataaatcaaa gactggtcaa agttattgat   1320
gacgttaatg aagaagattg gaatttattg gaaaagttat caatggacgg tactgaggag   1380
ttcttgaaag aggctcttgc attcgacaac gatgaatcag atgcacaaga cgatgccaac   1440
aatgagaaag aagacgatgg ggaagaattc tttcaacaaa ttgaaaccaa tttccaaccc   1500
gagggcgatt tctccttgtc tggtaatatc gaacaaacta tttccaagaa cttggtttct   1560
ggcaacatta agagcgctgt gaaaaattct ctagagaatg acttactaat ggaggccatg   1620
gtgatcgcat tagattcaaa taacgaaaga ttaaaggaaa gtgtcaagaa tgcctatttt   1680
gcgaagtatg gatctaaatc atcgctctcg aggatactat actccatttc taagagggaa   1740
gtagatgatt tggttgaaaa tttggatgtc tctcagtgga agtttatctc taaagcaatt   1800
caaaacttat atccaaatga tatcgcccag aggaatgaaa tgttgattaa attgggagac   1860
aggttaaagg aaaatggtca tagacaagat tctttgactt tgtacttggc tgccggatca   1920
ttagataagg tggcttcaat ttggttatca gaatttccag atttggagga taaattgaag   1980
aaagataata agacaattta tgaagctcat tccgaatgtc taactgagtt cattgaaaga   2040
ttcaccgtat tttccaactt cattaatgga agctctacca ttaataatga gcaattaatt   2100
gccaaatttt tggaatttat caacttaact acttccacag gaaatttcga actagccact   2160
gaattcttaa atagtttacc aagtgacaat gaagaggtta aaacagaaaa ggcacgtgtc   2220
ttgattgctt ccggcaaatc attaccggca caaaatcctg cgacagcgac gaccagcaaa   2280
gccaagtata caaacgccaa gacaaataag aacgttcctg tactaccaac tcctggaatg   2340
ccttctacta cttctattcc tagtatgcag gcaccatttt atggtatgac accaggcgcc   2400
tctgcaaatg ctctacctcc aaagccgtac gttccagcaa ccaccactag tgctcctgtt   2460
catacagaag gtaaatatgc gccaccaagc caaccttcga tggcgtcacc ttttgttaac   2520
aaaacaaata gctcgaccag attgaattct tttgctcctc cgcctaaccc atatgccact   2580
gcaacagttc ctgcaacgaa cgtatctaca acgtcgattc cgcaaaacac ttttgctcct   2640
atacaacctg gtatgcctat tatgggcgac tataatgctc aatctagctc tattccttca   2700
caacctccaa ttaatgctgt atcgggtcaa acgccacatc tcaaccgtaa agccaatgat   2760
ggttggaatg atttgccttt gaaggtcaaa gaaaaaccat ctcgtgccaa ggctgtatct   2820
gttgcccctc caaatatcct atcgacacca actccattaa atggtatccc tgcaaatgct   2880
gctagtacca tgcctccgcc acctctttcc agagctccct cttctgtgtc aatggtatca   2940
ccacctcctc tacacaaaaa ttctagagtc ccatccttgg ttgcaacttc tgagtcacca   3000
agggcatcca tatcaaatcc atacgctcct cctcaatcat cacaacaatt cccaataggt   3060
actatttcta cagcaaacca aacgtcaaac accgctcagg tagcttcatc gaacccctat   3120
gctccaccac cacaacaaag agtagcaacc ccattatctg gaggcgtgcc tccagctccg   3180
ttgccaaagg cctctaatcc atatgctcca actgcaacca ctcaacccaa cggttcctcc   3240
tatcctccaa ccggtccgta tactaataac cataccatga cctctcctcc tcccgttttt   3300
aacaaacctc ccactggccc ccctccgatt agcatgaaga agagaagcaa caagttagct   3360
agtatagaac aaaacccatc tcaaggtgct acttatcctc caaccctttc cagctcggcc   3420
tctccattgc agccttctca accgccaact ttggcttctc aggttaatac ctccgctgag   3480
aatgtcagtc atgaaattcc agctgatcaa caacccattg tcgacttctt gaaagaagaa   3540
ctggctcgcg taacaccatt gaccccaaag gagtactcca aacaattaaa ggattgtgat   3600
aaacgattaa agattctttt ctaccatttg gaaaagcagg atttattaac ccaaccaaca   3660
atcgattgtt tacatgacct cgtcgcatta atgaaggaaa agaaatacaa agaagctatg   3720
gtcatccatg ctaatatcgc tacaaaccat gctcaagagg gtggtaactg gctgacagga   3780
gtgaagaggt tgattggcat agctgaagcg actttgaatt aa                      3822
<210>  40
<211>  1929
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  40
atgtcaaaag ctgttggtat tgatttaggt acaacctatt catgtgttgc tcattttgca     60
aacgataggg ttgaaattat cgctaacgat caaggtaata gaacgacgcc ttcttatgtg    120
gcttttactg acacagaaag gctaattggt gacgctgcga agaatcaagc tgcgatgaac    180
ccacataata cagtattcga tgctaagcgt ctgatcggac gtaaattcga tgatccagaa    240
gtgacgaacg atgctaagca ttacccattc aaagtgattg acaagggagg taaaccggta    300
gtgcaagtgg aatataaagg cgagacaaag acatttactc cagaagaaat ttcctcaatg    360
atcttgacaa agatgaagga gactgctgag aactttttag gaacagaagt gaaagatgct    420
gtagtaacgg ttccagccta tttcaacgat tcacaaaggc aagcaacaaa agatgccggt    480
acaatcgcgg gcttgaacgt tcttcgtatc attaatgaac ctacagctgc cgctattgcg    540
tatgggctgg acaagaaatc gcagaaggag cacaacgtct tgatctttga tttaggtggt    600
ggtacttttg atgtctctct gctatccata gatgaaggtg tctttgaggt taaggctact    660
gctggtgaca ctcacttggg tggtgaagat ttcgatagta ggctggttaa ctttctagcc    720
gaggagttca aaagaaaaaa taaaaaggat ctaacaacta accaaaggtc cctaaggagg    780
ttaaggaccg ccgctgaaag ggccaagaga actctgtctt cgtctgctca gacatctata    840
gaaatagatt cattatttga gggtatcgat ttctatactt ccattacaag ggcaagattt    900
gaagaattat gtgctgattt gtttagatct acattggagc cagtggaaaa agttttggct    960
gattcaaaat tagataagtc acaaattgat gaaattgtac ttgttggtgg ttcaacaaga   1020
attccaaaag tacaaaaact ggtttctgat tttttcaatg gtaaagaacc aaaccgttcg   1080
attaaccctg atgaggccgt cgcttatggt gctgccgtac aggctgccat cttaacgggt   1140
gaccagtcgt cgacgaccca agatttactg ttgctggatg ttgcaccatt atctctaggt   1200
attgaaactg caggtggtat tatgacaaag ttgatcccaa gaaattcgac tatcccaaca   1260
aaaaaatcgg aagtgttttc cacctacgct gacaaccaac ctggtgtgtt gatacaagtt   1320
tttgagggtg aaaggacaag gacaaaagac aacaatctac tgggtaaatt tgagttgagc   1380
ggtattccac ccgctccaag aggcgtacca caaattgaag ttacatttga tatcgatgca   1440
aatggtattc tgaacgtatc tgccgttgaa aaaggtactg gtaaatctaa caagattaca   1500
attactaacg ataagggaag attatcgaag gaagatatcg ataaaatggt tgctgaggca   1560
gaaaagttca aggccgaaga tgaacaagaa gctcaacgtg ttcaagctaa gaatcagcta   1620
gaatcgtacg cgtttacttt gaaaaattct gtgagcgaaa ataacttcaa ggagaaggtg   1680
ggtgaagagg atgccaggaa attggaagcc gccgcccaag atgctataaa ttggttagat   1740
gcttcgcaag cggcctccac cgaggaatac aaggaaaggc aaaaggaact agaaggtgtt   1800
gcaaacccca ttatgagtaa attttacgga gctgcaggtg gtgccccagg agcaggccca   1860
gttccgggtg ctggagcagg ccccactgga gcaccagaca acggcccaac ggttgaagag   1920
gttgattag                                                           1929
<210>  41
<211>  2082
<212>  DNA
<213>  Saccharomyces cerevisiae
<400>  41
atgagtactc catttggttt agatttaggt aacaataact ctgtccttgc cgttgctaga     60
aacagaggta tcgacattgt cgttaatgaa gtctctaacc gttccacccc atctgttgtt    120
ggttttggtc caaagaacag atacttgggt gaaactggta agaacaagca gacttccaac    180
atcaagaaca ctgtcgccaa cttgaaaaga attattggtt tggattacca ccatccagat    240
ttcgagcaag aatctaagca cttcacctct aagttggttg aattggatga caagaagact    300
ggtgccgaag ttagattcgc tggtgagaaa catgtttttt cagctactca actagctgcc    360
atgttcatcg acaaagtcaa ggacaccgtc aagcaggaca caaaggcaaa tattaccgat    420
gtttgtattg ctgtcccacc ttggtacacc gaagaacaac gttacaacat tgctgatgct    480
gctagaattg ctggtttgaa ccctgttaga attgtcaacg acgttactgc tgccggtgtt    540
tcttacggta tcttcaagac tgatttgcct gaaggcgaag aaaagccaag aattgttgcc    600
tttgttgata ttggtcactc ttcctacacc tgttctatca tggccttcaa gaagggtcaa    660
ttgaaagtct taggaactgc ctgcgacaag cattttggtg gtagggactt cgatttggct    720
ataacagaac atttcgccga tgagttcaaa actaaataca agattgacat cagagaaaat    780
ccaaaggctt acaacagaat tctaactgct gctgaaaagt tgaagaaagt tttgtctgct    840
aatactaatg ccccattctc tgttgaatcc gtcatgaacg acgttgatgt ttcctctcaa    900
ttatctcgtg aagaattaga agaattggtc aagccattgt tggaacgtgt tactgaacca    960
gttaccaaag ctttagctca agccaaatta tctgctgaag aagttgattt tgttgaaatt   1020
attggtggta ctactcgtat cccaacattg aaacaatcca tttctgaagc cttcggcaag   1080
ccattgtcca ccactttgaa ccaagatgaa gccatcgcca agggtgccgc ctttatttgc   1140
gccattcact ctccaactct aagagttaga ccattcaagt ttgaggatat ccatccttac   1200
tctgtctctt actcttggga caagcaagtt gaggacgaag accacatgga agttttccca   1260
gctggttcat ccttcccatc tactaaattg atcactttga accgtacggg tgacttttca   1320
atggctgcta gctacactga catcacacag ttaccaccaa acactccaga acaaatcgct   1380
aactgggaga tcactggtgt tcaattacca gaaggtcaag actctgttcc tgttaagtta   1440
aagttgagat gcgacccctc tggtttacac acaattgaag aggcttacac tattgaagat   1500
attgaagttg aagaacctat tccattacca gaagatgctc cagaagatgc tgagcaagaa   1560
tttaagaagg ttactaaaac tgtaaagaag gatgacttaa ccatcgttgc acacaccttt   1620
ggcctagacg ctaaaaagtt gaatgaatta attgaaaaag aaaatgaaat gcttgctcaa   1680
gataagctag ttgctgagac agaagaccgt aagaacactc ttgaagagta catctacaca   1740
ttgcgtggta agttggaaga agagtatgct ccatttgctt ccgatgctga aaagacgaag   1800
ttacaaggta tgttaaacaa ggccgaagag tggttatacg atgaaggttt cgattccatc   1860
aaagctaagt acattgccaa atacgaagaa ttggcttctc taggtaacat tattagaggt   1920
agatacttgg ctaaagaaga agaaaagaag caagctataa gatctaagca agaagcatcc   1980
caaatggctg ctatggctga aaagttggct gctcaaagaa aggcagaagc tgaaaagaag   2040
gaagaaaaga aggacactga aggtgatgtt gacatggact aa                      2082
<210>  42
<211>  777
<212>  DNA
<213>  Pichia pastoris
<400>  42
atgtcaagag aagattctgt ttatttagca aaactagctg agcaagctga gcgttatgag     60
gagatggtcg agaacatgaa gaccgtcgcc tcttccggct tagagttgtc tgtcgaagag    120
agaaacttgc tttctgttgc atacaaaaac gtaattggag ctagaagagc ttcttggaga    180
atcgtctcct caattgaaca gaaagaggaa gccaagggta accaatcaca agtgtctttg    240
atcagagaat accgctccaa gattgagacc gaattggcca acatttgtga ggatattttg    300
tctgttttga gtgagcacct tattccttct gccagaactg gcgaatccaa ggtcttctac    360
tttaagatga agggtgatta ccaccgttat ttggccgaat tcgctgttgg tgacaagcga    420
aaggaagctg ctaatttgtc attggaggct tacaagtctg cctctgacgt tgctgttacg    480
gagctacctc caactcatcc aattagattg ggtctggctc tgaatttctc agtcttctac    540
tacgagattc taaactctcc tgaccgcgcc tgtcatttag ccaagcaagc tttcgacgat    600
gctattgctg agttagaaac cctatctgaa gaatcttaca aagactccac tttgattatg    660
caactgctgc gtgacaactt gactttgtgg acctcagaca tgtctgaaac tggacaagaa    720
gagtcatcca atagccaaga taagacagaa gctgctccca aagatgaaga gtgaata       777
<210>  43
<211>  1500
<212>  DNA
<213>  Pichia pastoris
<400>  43
atggctagaa agacattggc tgaaacattg gcagaattgt ctcaaccagc gtctggagat     60
tttgatatag aagaccaaga aggaggagca gtacttgact atggagataa tagttctttt    120
ggctccgaga gtgaagagga taaaagtaac cactatgtta aagttggcaa gtcaaggata    180
agagagaacg cagttaaatt gggaggacaa tacgagggaa aaaagagtag tagagccgat    240
gtttttggag acgaggacga tgaggaggag gacgatgagg atgttgaaca ttcggaaact    300
gaagatgcac tttcggtttc aggatcagag tccgaatcgg atgaaaaaaa tagtgatcaa    360
agccaaggtg attctgagag tgaagaagaa tctaactcag gtgaagatct agactacaag    420
agatcaaaac tacagcaact tataagctcc gaaaggaaaa ccattgtaaa ccaattatca    480
acttccaata aacaagatgc actgaaaggg tttgcagtgt tgaatcagca gatacagtat    540
gatcaattgg ttgacctcag aataaaatta cagaaaggat tagtagcatc gaatggtcta    600
cccattaaca aagaatatta cgaacagaat aaagcaccaa agtcttccaa acacctggat    660
aagctacaag ataaactata caatttattg gatgtcactt tagaactgag aggcaagcta    720
ttaaacaaaa gcaagattgt gagccaagag tttcccccta ttccaagtaa gaaacgtagt    780
ttacagcatt atttggagga atcttccaag ttggataaca tagttaatga atatagaagg    840
aacgtcctcg ttaaatggtc tcaaaaagtc caaaatgctt ccggagcaac tgctttgagc    900
tcatccaaat tcaaggctat taaccaagat agttcgactc aagtggacaa ctatttggca    960
gacatggata gattaatcaa aagaaccaga ctcaacagaa gaagcgtagt gccattagga   1020
tacaccgaga cagaagaagt agtagatgat gatgaattga tcgacaacga taaagataac   1080
aatgagacca aatacttcag caacattgac cgatctttga aggaaaacaa atatatctat   1140
gatgatgacg atttctatag agttcttctg aacgatctag tcgataagaa agtttctgat   1200
acacagaagc tgacatctac atcaactgtt attacatttt cgaaatccaa attgcataaa   1260
agttatgaaa gaaaagcgac taagggtcgt aagctgaggt atacagttca agatccatta   1320
ttgaattttg aagcctccaa cccacatgcc tacaagtgga acgactacca aattgacgag   1380
ttttttgcgt cattatttgg gcaaaaggtc aacatgaacg aggatgagca taacgaagag   1440
gtacaaggtg aatcagaagg agaggacatt ttgaaggatg atatcaaact gtttggataa   1500
<210>  44
<211>  2351
<212>  DNA
<213>  Pichia pastoris
<400>  44
atggactttg tatatgagta ctcagatgct acccctagtg gcacatttga tgacccattg     60
cctgcagagc ccgaaccacc attcaatttg tcaaacttaa actcgtacaa agatgatttg    120
actaaaaaat tctccaaaat gagcattctg aaaagtctga aaaatgacac caattcagtt    180
gacgatgtcg acgactcaca atcgatctcc aatgacgggc agagggctta taaatacgcc    240
aatcagtctc tggatctggt taaccagcac accactaata aatcaatcag aaccaccagc    300
gatgaacaac cttcggtgtc cactgttttg agcaacagac tgagcagagt gctcaataat    360
actaattacg acccttcaac caaggaacta ctctccattg tggagaagaa aataaaagaa    420
gatacggcgc atgaatacga caaagttact gacccaagtt ttgttggaaa ccttgctaga    480
agaaagttgc gtaacgacat tgaacatgat gttgtagatg ccaacttcaa tttcttgaaa    540
caattgcaac ccttaagaaa gaccttgggc cagattgaaa gtgacttgaa tgaaatgaac    600
gagctcaaca atcaaatcac tgaaaagttg tcctctagag ttgaagatac cactaggttg    660
gataattcca tacacgagtt gcatgcaact tccaagatta tttccatcaa aaagaagctt    720
ttgcagaatt tccagaaccg ctatactctc tcccatttcg aagcacatca attagagttt    780
ggtgaaattg acgattcctt tctagaaata ctgaaaaaag ctgagtcaat tcatgatgat    840
tgttcaattt tgttaaccat ggagaatgct actgtgggta ttaatattat gaacgacatg    900
aaaaagcttt ccaataatgc catcgacaga ttgtcgacat ttgtcaccaa acatttttct    960
aggttaagtt cgtccaacaa tacctccgcc tccatagagg ataaggcatt cctgaaaaga   1020
tctatactct tcatttccga aagatacccg gagcagctct ctggaatcac caaccaaata   1080
gtcgaatcaa ggtcaaagtc tttgcttgac gagttccaaa tacaattgaa tggttatgca   1140
gattcagcat caagaaacga aagggatgtt aataaaccat tgttcctttc cgcatatgat   1200
tcagaaaggt ttctcggaga tttacttgct tatattcatg gcacaattgt taatgaaaga   1260
gaaaccgtcg aaagcttgtt cagtttgcaa gatgaggaga aagataatat cgttttgaca   1320
acactcgtag agtcaattgt ttcaaagaac atcgaatctc tagctacccc cctgaattta   1380
aagattgaac agatcatcag aaatgagtct aagctgacag cgatccaagc tttttatgac   1440
ctgctctcac tttattccat gatgcttgaa aaaactttag gttctaagaa tgcccttttg   1500
agtacaatca actctttaaa agtttcggct ttgggtaaga ttcaaagttc aatcaacatt   1560
aaacttaaaa acatagagcg gactgccaat gagagcatgt catattataa tgaagatgaa   1620
caactagatg gtacaaacca caactttgtt tcagaaactc attacattga agaaatcaca   1680
cctgagctag ctgtgcctga ttggttgatc aatttctatg gtgacgtact tcccatcttt   1740
gataatgaaa aggtgacaaa tgccaaagaa ctgtatgagg atttactcaa atactgtttt   1800
gaacaaatca ttcaacttat cgagaaacaa atagctcaga ataaattgaa tgatgctaga   1860
gagatattga ttttcaaatc aaactgttac gattttgttt attccaaaat tgtgaccctg   1920
aacatcttta aggagaaact ggatcgatta gaggtaatga taaaggaatg cgaatcaaaa   1980
ttgaccgaaa ttcagtacac ttatcttctc aaacaatcag ggttatatga tattcacaac   2040
cttgtcaaca tgatatcctc aactagggaa gatttctttg acgtctccgt ttatgaacca   2100
attacggaga actcactatt caatggtgac aaattcaaag aaatatcaga tcgccttcaa   2160
gattttcttc caattgcatt aattgattac caagaggagc gattgttgta tctattacct   2220
cccacgcttg ttaactctat cattcaaaac tcctctgtgg attttgtcaa cttttatttc   2280
aaattatcgt tgatcgtgaa ggaatatttg aaagccagtg aaggatgtct cagatgggat   2340
gacatggagg t                                                        2351
<210>  45
<211>  1443
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<222>  (1)..(546)
<223>  partial nucleotide sequence from the 5'-end of COY1
<220>
<221>  misc#feature
<222>  (547)..(1443)
<223>  partial nucleotide sequence from the 3'-end of COY1
<400>  45
aaaagttaag taatgagttg gttagctaca gatcgataac aagaggacat ggttaattca     60
attcaggaac tggaagagaa gcttgcgttt tctcaaaagc aagtagagca gttacagcag    120
ttaaaccagg atttggagaa ggagactagt gtggaaaaat gggatgcaat ttcaatgatt    180
tctgccaggc cggacacttc aatacaggac aattcgttga ttacaatggt atcacaacaa    240
agagatcgat ttaagcaaag gaacaaagat cttgaaaaag acgttagatt acaattgaac    300
aaaatttctg agcttcaaag aaaggtccaa tcactttctt cagacaataa tcaattatac    360
gagagaatca ggtttttgtc atcctatgac agtaataaga atcagtccaa ggagagccag    420
tcggaagagt actacaagag aagttacgaa gacaaattgc atccgataga acaatttagc    480
atattgcatc agcctgcact taattgtgat gtcaatgacg atgtatgtga tgaatatcca    540
taatgaaatt cataccgttg tcacgtaatc gcgggggtag tgtgcatcgc atcgtattgg    600
agacatctgt ctgttttctt ccctcacatc gaaatacaac ttcaccatga ctgacgctga    660
cttccaagta gtatttgaag cgtggcaagc tgtcgatcta cagggtgtta agaagcttgt    720
agatgatgag gcaaaagaga ttgaaagttc gaagtcttca agtttggatc aaagaaagca    780
gttgagtttt aagacgaagg agttcaagaa attggacgat gagcgtaaat tgacacaatg    840
gaggtcgttg ttgaaggagt atcaaaacta cattgatgat ttgaccaagg gaaataatcg    900
tgttgtacag acattcttgg aattgcataa agtagtggtg gatttgaagg atcctacaag    960
tactttgagc aaggagcaag agacgaatac cgaattacag aaagctgtga aaaaactttc   1020
cacagaactg aggcattcag aacaacactg ggcttccgag aagaaaggat tggaagaaaa   1080
atttaacgta cgtaaaaggg aaacggaaga gaaaagtctt gatcagatta agacagccca   1140
aactgaaata gtccaattga gggatgagct gaagcaaaaa tcttcagaaa atgaagagct   1200
tcaagtggtg attgaaaccc ttgatgccaa gctaaaaaag aacagccaag gacaaaataa   1260
tgatgataca tactccaatt atgacatgtt aaatagagat ttggagtcca ataaactaaa   1320
gatccttgaa ttggaaaggt tgaacaactc tctaaaggag gaattagcaa agaaagatga   1380
caaagcctac caggagaggg tcaccgaact cgaaaaggag agtgtggagt atctctctta   1440
aag                                                                 1443
<210>  46
<211>  473
<212>  DNA
<213>  Pichia pastoris
<400>  46
gtcctgttta tgctccattc tttggatcca ttggttgtgc tgcggccatc atctttacct     60
gttttggtgc cgcctatggt actgctaagt cgggtgtagg tatttgtgcc acctgtgtct    120
tgcgtccaga cttactgatc aagaatacag tgcctgttat tatggctggt atcattgcta    180
tttatgggtt ggtggtgtct gtgttgatct cttcatcgtt gcaacagaag caggctttgt    240
atactggctt tatccaattg ggtgccggtt tatcagttgg tctgtcaggt ctggctgctg    300
gttttgccat cggaattgtt ggtgatgctg gtgtcagagg tactgctcaa cagccaagac    360
ttttcgtcgg tatgattctg attttgattt ttgctgaagt tttgggtctt tacggtctga    420
ttgttgctct tctactgaac tctagagctt cccaagatgt cacttgttaa agc           473
<210>  47
<211>  3466
<212>  DNA
<213>  Pichia pastoris
<400>  47
atgttctcaa aactttccca gttatcccag aatttaggcg aagagctctc taggattaat     60
gaggaggttg ctgcctctag aaggaaccaa ctaaagaaaa gcagggattc ggagagggat    120
acaaagttcc ttaacatcaa aactcccgat cctgaagctc tacaacaacc gggtcatgag    180
gtgaatgaag gcgctgaaac cgaaacagat gctactgagt caaagggcca agtggttcca    240
aacacaaata tacacttcaa tgatctgcct atggagatta gggcccgctt gaaaaagttt    300
gcgaaatatg agcagaaata tccgttgttg ttggacgctt acaaaactga gaaggccaaa    360
tctgaaatag ttcatgcttt tgaatcaact ttacaagaag tcactccttt gcagacaatt    420
ggagaaattg aacaattcaa agactttatc agcaatatga cccaaaaggc taaattaatg    480
gatgaagaat tgagagccaa aactggcgag ttgaatggcc taaaaaacga agtgacagaa    540
atgaaagaga aattgaaggc tgttcaaggt gagatgaaag ccaagtctgc tttagcagaa    600
gaatctgcga tgaaagccga tcaacttagt gtggatcttg aacgagttgt gagtgagcta    660
gaaaatttga aaaaggaaag ggaagagatt gttacggagc gtgatgaggc aaccaaggaa    720
cgtgacgagt caacaagaga aagagatatc attctagaag aagtcaaatc taataaaaat    780
caagaacttt tggaggaata caagtctgag ttagaagaag caaagaacgc tcttgcattg    840
agaactgagg agattgaaaa tctaaacttg aagttggagt ctgaaaagtc ggcaaagtta    900
tcattagagg gtgtagcaga tgagcgagat ggccttaaag caaagttaga agcgcaaaca    960
acttccttcc aggaagaatt agaccaactt tctcaagaac gggatcgttt gaattctcaa   1020
ctaacaatag gagaaaagtc acagatagaa attgagcaag aaaaaaatga gctcaagagt   1080
cagtacaatt ctgagattaa gtcatcgctt agtaaactgg aatccgtaat taaagaaaga   1140
aatgagctac aacagcaatt ggaatctcaa gagtctttaa ctttcgaagt ggataagctc   1200
tctaaagaga gagatgagct aaggatgcag ttggatagag aaaaagaaaa ttctgcaaag   1260
gctagcatca cgccccagaa ctttgaggtt aaaactaaag ttgaatccaa caagaacatt   1320
gaagcacctt tatctgaaga actcaggcaa gtcaccagag aaagagatga gctaaaagct   1380
cagttgttgc tcattcaaaa gaacccagga ccaagtaaga agaccaatga aggaaaccgt   1440
aacttagagc ggaacggtga aaaaaagtcc catgaccaaa atggtgctga cgatgatctt   1500
atcgaacgga agggtgaaag cggtacagat gattcaaagg ataatcagct gaaactaata   1560
gagcagtcaa ccactattac gatgttgaat gaagagattg aaaacttgaa agatatgctg   1620
cgtgacgtcg gagacgatct tgtagtggct aaagacaagc tttcacaggt ctctgctgtt   1680
gatgaaaaaa aacagcacgc tcttgaaagg gaactggaat cctcaaagtt gaagcttgct   1740
gagattgaaa aggattacaa cgacgataga gttgacctca agaatgaatt gaaactcgtt   1800
accgaggaaa aggaaaatct tgaacatgag aatgaaacat tgagtcaaag cttgaccgag   1860
cttgaaaagc tgaaacaaga agtcaaggag aaagctcaag ccgttcagaa ttatgaatct   1920
aagtattcga cactatcgga tgaattatct ttagtcctct ccaaacgaga tgaattggaa   1980
aaagacaagg aaagtttcag gctgaaattg aaagatttgg aacagaaaaa ttccgagact   2040
gaacaacaag aggaatcgca gagaactgga actgcagaaa tggaagagga gttgcagact   2100
ttgaaaaggg agctagaagc aagctcccag cgcattgagg attataagca aaagcaacta   2160
gaactagatg atgaaatatc tcttgtacgt tctcaaaaag atgaattaca gaaaacgata   2220
actagcctgc aagaagacct ggggaaagag aaagagaatg ttaagttact ccgtgaaaat   2280
atcattgccg aagaaaaagc caaaaattcc caaaaactgg cagaaaatgt ggcgcagcta   2340
gacaaattca aaaagcagga gatatcactc aagcttgaga ttgcaaacct tgaaaatcta   2400
aatgctgaaa aaggctcaaa gatcaaaagt ttggaagagc atatcactta tcttaataca   2460
gagaggcaat ctaattatga tgagaatcag aaactgatct cccatactaa tgagaaatgg   2520
aagcaggatt acacggagtt agtcataaaa ttgaacaagt gtcagtcaga gaacaataga   2580
cttacaaaag aattgaacga atacaaagat aaactaaaag atatgaacac ctcaaagctg   2640
aacagtagcg agacaatcga gtcaatcaga agacaatgcg aagaacttaa aatgatgaat   2700
aatgaatatt ctttgaagat tgaaagtcta catgaagaac taagttcttc gagttcaatt   2760
ttacaggaac gttccagaga aatgaatact atacgtaaac tgctagctga tactgagtcc   2820
aaatgtgacg aaagaatcaa acagttaaaa gcaagaattg ataggttaga agaagaaaag   2880
gagacgacta gccatgaaag ctctgtccag gcaagaaagc tgagtaaaac aatcgaccag   2940
ttaaagaaag gcaagaatga attgtcagtg cagctagaac aatgtaagct agagctggaa   3000
catctgaaat ccgtcccatc tagagtggat gttgacaata aaaacggtgc ttcaaatgaa   3060
aacagtgatg aaaaccaatc tgatattgaa tctggaatta tcgaacagct cagaaactcg   3120
ctaaagggat atgaagaaca actaaaacaa taccaagatt ccaacgtctt actcaagaag   3180
gttaacgaag agcagttgct gaagttcgag agactgcatt caaatttcaa gattgtatct   3240
aaacaatata gaatgctgaa agatcaaaag gacgaagtca atacgagaag tagaaacaat   3300
tcagttataa gttcaacgag cgcggggagt gatgaaaatg agagagataa agttgcctat   3360
attaagaacg tccttctagg atttttggaa cacaaagatc aacgagctat gctttttcct   3420
gtagtgaaga tgctacttat gctggacgat gatgaagaga gaaggt                  3466
<210>  48
<211>  3324
<212>  DNA
<213>  Pichia pastoris
<400>  48
atggatagag aacagggtat tctgccacag gatcccttct ccaactcggt gcatgtacca     60
aagttgagag cttcttctgg tggccagcca cagaagcctg taatacaaaa ttctgctcct    120
gctactgcta ggatgcttcg caatgcaagt tcaagtacgt cagcagcttt gttgaaagaa    180
ttaaacacac atgaacactc tcaacgtcaa catactccac agaaacaacc atcattggat    240
gccccggcag cattggttcc agttgaatct gccacaaaac aattccaccg aacctccatt    300
ggagactggg aatttagtaa tacaattggg gcaggctcga tgggtaaggt caaagtcgcc    360
aaacatagag tcactcacga ggtatgtgcc atcaaaatag tcattaggtc agccaaaatc    420
tggcagagaa atcaccaaaa cgatccagaa cctgaaactg aagaaaaaag aaagaagctg    480
cgtgatgaat acaagaagga attggaacgc gatgaacgta ctgtcagaga ggcagcacta    540
ggaaaaataa tgtaccaccc aaatatttgt cggttgttcg aatgctatac aatgtctaat    600
cactactaca tgctttttga aatagtccag ggggtacagt tactggatta tattgtttct    660
catggcaaat tgaaggaaac acgcgttcgc cagtttgcca gaagcattgc ttctgcttta    720
gattactgcc attctaataa catcgttcac agagatctga aaattgaaaa cataatgatt    780
aacaaacagg gtgaaatcaa gttgattgac tttggccttt ccaacatgta tgatagaaga    840
aatctcctga aaaccttttg cggctcccta tactttgcag caccggagct tttgtcttgc    900
cgtccttaca ttggtcctga aattgatgtc tggtcttttg gggttgtatt atttgtcctt    960
gtttccggta aggttccctt tgatgacgac agcgtgccaa agcttcatgc taaaatcaaa   1020
agaggaaaag ttgagtatcc tgagtttatt tcacctttat gtcattcatt gctatctcag   1080
atgttagtcg ttaatccaga tcatagagtc actttgaaag ctgcaatgga gcacccttgg   1140
atgaccttag gatttgcagg gcctccatca aactatctcc ctcagcggtc acctattgta   1200
ttaccgttgg atttaagtgt agtaagagag attgcaaatc tgggtttagg aaatgaagaa   1260
caaattgctc gagatatcac aaacctgatc tcgagcagag aatatgaagc gtgtgttgag   1320
aggtggaaac ttgatcaaca gaaagctaat atcaagggct attccgcgcg tgacgattct   1380
gctatcatcg ccttccaccc gttactttca acgtactacc tcgtggatga aatgaggaag   1440
aggaagctag caaaaggtgc tctcaaggga cagacctcgg tattagacac tgtcaaggtg   1500
tctccagaca ttccaaagac accagctatt ccccagaaac tagaaactac ggatgtggaa   1560
cagccattgc ttgccactgt cccacctgct tatacatctc cgcatggaca gccagctgaa   1620
ctggaagcga tgattgaacc ggcacagcca ttatctagtg ctcatccttt cgagatggat   1680
atgacgcagc aacaacatgc tagcagaaag acccatatca agcatgctcc agaacgacaa   1740
gatcgtggcg gctataatgt acacaagaat aactctggtg gtcttaactc tttattccga   1800
agactcagtg gaaaacgacc ccataagaat gaggctgaat gggagccttc atctccccca   1860
cctcaagttc atccattttc agttaatgat gcggacagga cttcagtacg tggcgtttca   1920
ccaattactc aaccagctgc tgtgaagaat gtgacctcca ataactccaa aaactacctg   1980
gaccctgttg atgatagtaa attagttcgt cgtgtaggaa gtttgagaat taccaacaaa   2040
gaaaagcaac aagtgacatc tgactttccc cgactgccca attttacgat tccagagcaa   2100
ccgcctaaga atgctcccat accgatacat gcccaaccta ccactacagg tacaaccttt   2160
caatccaatg atcatgaaat caaaaagaag ttacaggctt cgactagtcc aaacgaacaa   2220
cgtgggcctc caacattggc tcctagtcaa cagagacggc tacatcccac tgcgagagcc   2280
aagtcacttg gccattctcg caagcaatcg cttaatttca aattcggagg accagcaaac   2340
aatcaattac ctgcgttgcc tactaaagaa aattatgatg tgtttgaaga tgcccaaatt   2400
accgataaca atttattaaa cccagaaggg aaatactctg ctaatactaa cgtgcatatc   2460
aaaccaatga cagaatccca aattttattt gaggcagaac atgctccacc tggaactatg   2520
ccctcagttg agtaccccag gaccttgttt ctcaaaggat ttttctctgt tcagactaca   2580
tcctcgaagc cgttacctgt tattcgatac aacattatag cagctctctg caaacttaac   2640
attcaattca ctgaagttaa cggtgggttt gtttgcgttt acagaaaaac tgaaaattta   2700
caaattgggg atatcagatc tccagttata gagtcaagag tgaccgatga cactgactcc   2760
gatgttgcaa actcttccaa attgtcatct tcgtcaacag ccaataccag agtcaatgtt   2820
attgaggatg atagttcatc gccgtcctca gcaagattga aacatcgccg aaagttttct   2880
cttggaaacg gaatccttaa ccatataagg aaacccacgc ttgacgggac agaatttgat   2940
gactacgatg caaccgtaaa tacccctgtt actcctgcac ctgcaaatgt tcattctcgt   3000
tcatcgtctt atcataccga gagtgataat gagtccatgg agtcgctgca tgatataaga   3060
ggtggcagtg atatgatctt gaaaaatgtt ccagaaagaa atgctagaca gatagacaca   3120
gtcaaggaag aggaaacaga tgatgatgat cttggtagta tcaacgaagg atcaacacac   3180
cgtacacctt tgaaatttga aattcatatt gtcaaagtcc ctctggttgg actatatggt   3240
gtgaggttca agaaaattct gggaaatgct tggatttaca aaaggttggc gtcaaagctg   3300
ctacaagaat tgaatttata gttc                                          3324
<210>  49
<211>  3086
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<222>  (1)..(2215)
<223>  partial nucleotide sequence from the 5'-end of SEC31
<220>
<221>  misc#feature
<222>  (2216)..(3086)
<223>  partial nucleotide sequence from the 3'-end of SEC31
<400>  49
atggtgaaaa taagtgaaat aaaaagtact tcaacatttg catggtcgtc tgtagactct     60
aatgtcttgg ctacagggac cttggctggg gctgttgacg actcattctc taccacttcg    120
tcattggaac tttgggatgt cctgaacacc tcagctccca tattccgaac caatgttggt    180
gcaagatttc atgatcttgc gtggagtaat ccaatctcta agtaccagag aggactactt    240
gcaggtgctt ttgataatgg aacaattcaa ttgtgggatt cctcatcatt gctgaatgga    300
tcatctgaca gtttaataga gctaaagaaa cacactgcgc ctgttaaaac aatatctttc    360
aatcctacag agtcacagat atttgcatct ggtgcttcca atggccaatt attcatttgg    420
gatataaatc atctttcaga gcctatttca ccgggtgctt ctactacccc tattaatgac    480
ataaactcca ttgcttggaa ctccaagata cgtcatattt tggcctctgc tggaacctca    540
ggctacgcat ccatttggga tttaaagacc aagaaagaac tattgaactt gagttacact    600
gctccatcag gtcaaagagc taacttaagc accgttgcat ggcatcctac taattcgaca    660
agtgtaataa cagcttctga ttcggacgct gtaccattga taatgacttg ggatttaagg    720
aatactaatg tacctgtagc tactcttgaa ggtcatcaaa agggtgtatt gtccctggat    780
tggtgttcgt gggactcaga actattactt tcttctggaa aggataactc taccctattg    840
tggaatccca tcagaggctc tttgttagcg gaatacccaa ccaccactaa ttgggccttc    900
aagacccgct tttcttccaa gcttcctgac atttttgcaa ccagttcatt tgatggtaag    960
ataacggtgc agaccttaca ggatactaca cctgcagagg ctcaacaagc aaaagctatc   1020
aacgatgacg aattctgggc agacctgtcc aacagcgata agaaacatcc taatttttta   1080
caacgtcaaa ctccggcctg gcttaaagtg ccttccagcg tttcatttgg atttggtgga   1140
aagattgtaa aagtttccaa ggcctctgat aaccagtcaa ttgttgtaat tgataatttc   1200
acaactaacg atacgctggc caagtccact tcccttcttg caagcaccat cagcacaaac   1260
gattaccaaa ctcttgtcga cgaaaaactt cgtaccgaag caaataacca cgactggcaa   1320
ttattgaacg atctattaaa agcggatgat gtgaaagatt atttcagatt tcagattgtg   1380
gatccttccg tattgaaaca tgacaagtct gaacaaaagg ttgaaaacgg acaagacata   1440
tttgaaaaca ttgagcaaac tgatgaagac tttttcaaca atcttgaaag ggaaaagaat   1500
tcagtttctg tcaacattcc atcatactct ccaactgctc tcagccaggg actaatccag   1560
gaggctctag tattggcttt aggtgcatct gaatcattac aggctaaagt taggaatgcc   1620
tattttaacc aaacgcaaaa gtcctctcta ccaagattga tttacagtgc tactgctaac   1680
gatgttaatg acctcgttgc taatggtact atctctggtt ggagggatat agcagctgct   1740
atttttgctt actctacgga gaaagaagag ttttcaaagt tcattgtgga actaggtgat   1800
aggctattag ccagttccct ttcagataga cgctctgatg ctctgctttg cttccttgct   1860
ggtggtgcgc tcaacaaggc gtctacaatt tggaatgccg agttgagttc tcgtgaagag   1920
gttctcaaat ctgaaaaccc tcagctctca tcttatgaag ctcataatat tgtgttgact   1980
gagtttgttg aaaaaattgc cgcattcaag tatgcattaa ggatcagcaa taagttcagt   2040
gggcagggcg ttaatacgtt gaacaattca ttcctagagt ttgcttcttt ggtgtcatct   2100
caagggcaat ttgatttggc cttgaactta ttggagaact tatctaccga ggatgaagac   2160
attaaacttg agataaagcg aatctcaaca gcatcaggaa aaactctttc cagtatcctc   2220
ccncgctcac ccctcccgnc cttcccctat cgcatttcca aaggtcttct cgtggaggct   2280
cattctccgt tccacctcct aatccatacg taggtagctc agtgaatggg aatggaggag   2340
tgcacggagg cgcaccagct atccctgttg ccaacaaccc ttatgctaac aacaatcaaa   2400
atgcatcata tggccaagca aacggtccac taaatggatt tgtacctccg ccaccaatgc   2460
ctgagaaaat gggaggactt tcctcacaga attaccccaa gagagcagca agtagagcaa   2520
atagcactgc tggatatgcg ccatcactaa gatcgcccag tgtgcaacaa tttcaaccac   2580
caccacctcc ggcactagct caacatgtgc aaccgccacc acctcctgaa ctagttcagc   2640
aggtacctcc acccgcgccg tctgtacaac accaagtatc acaaggatct caaggatctc   2700
aaggtgggcc ccctgcacaa caacagacca gatttcccag tggagataga tcacatataa   2760
gtgatgaggc tttccctatt tatgagtacc tgagtaagga gttggaaaat gttaagccta   2820
agattccaga aagatttacc aaacaactcg tagacgctga gaagagattg aatatcttgt   2880
ttgatcattt gaataacaat gagctgttaa ccgctcctac gattacactg ttgtctaatc   2940
tttcaaagtc tctagctgac catgacttta agactgctga atcgttactg attcaaatta   3000
ctaccattca taacaacgag gcaggaaact ggagcgttgg tgtgaaacgt cttatccaga   3060
tgtcctcggc tctgagtagc taagaa                                        3086
<210>  50
<211>  1974
<212>  DNA
<213>  Pichia pastoris
<400>  50
atgggtaaat caattggaat tgatttgggt accacatact cttgtgtggc acattttgct     60
aatgatcgtg ttgagatcat agctaacgac caaggtaaca ggacgactcc atcgttcgtc    120
gcctttaccg acactgaaag attgattggt gatgctgcaa agaaccaagc tgccatgaat    180
ccagctaaca ctgttttcga tgccaaacgt ttaatcggta gaaaattcga cgacccggaa    240
actcaggccg atattaagca cttccctttc aaagttatca acaagggggg aaagcctaat    300
atccaagtcg aatttaaggg tgagactaag gttttcagcc ccgaagagat ttcctccatg    360
gttctaacaa aaatgaagga tactgctgag cagtatttgg gtgagaaaat caacgatgca    420
gttgtcactg ttcctgctta cttcaatgac tctcaaagac aagccaccaa ggatgctggt    480
ttgattgctg gtttgaacgt tcaaagaatc attaatgagc ccaccgctgc cgcaattgct    540
tacgggttgg acaagaagga tgcaggccac ggtgagcaca acattctaat cttcgatcta    600
ggtggaggaa ctttcgatgt ttctctacta tctattgatg agggtatttt cgaagtcaag    660
gccaccgcag gtgacaccca cttgggtggt gaggacttcg ataacagatt agtcaaccac    720
tttatcgccg agttcaagag aaagaccaag aaagatcttt ctacaaacca gagatccctt    780
agaagactaa gaaccgcttg tgagcgtgca aagagaactt tgtcttcttc tgctcagacc    840
tccatcgaga ttgattcttt gttcgagggt atcgacttct acacctcgat cactagagct    900
agattcgagg agctctgtgc cgacttgttc agatccacca tcgagcctgt tgagagagtc    960
ttgaaagact ccaagttgga caaatctcaa gttcatgaga ttgttttggt tggtggttct   1020
accagaattc caaaggttca gaaattagtt tctgactttt tcaatggtaa ggagccaaac   1080
aagtccatca acccagacga agccgttgca tatggtgctg ctgtccaagc agctattttg   1140
tctggagata cttcttccaa gacacaagac ttgttattgc tggatgttgc tcctctatct   1200
ttgggtattg aaaccgctgg tggtatcatg accaagctga tcccaagaaa ctccacaatc   1260
ccagccaaaa agtcagaaat cttttcgaca tatgctgaca accaaccagg tgttttgatt   1320
caagtctttg aaggtgagag aactagaacc aaggacaaca acctgttggg taagtttgaa   1380
ctttctggta ttcctcctgc tccaagaggt gttcctcaaa ttgaggtcac cttcgatatg   1440
gatgccaacg gtattttgaa tgtatctgct gttgagaagg gtaccggtaa gactcaaaag   1500
attactatta ccaacgataa gggaagattg tccaaggaag acatcgagag aatggtttct   1560
gaagctgaaa aattcaagga tgaagacgag aaggaagccg agagagttgc tgccaagaat   1620
ggcttggaat catatgctta ctctctgaag aactctgcag ctgaatctgg attcaaggac   1680
aaggttggag aggatgatct tgccaagttg aacaagtcag ttgaagagac aatatcttgg   1740
ttagatgagt cacaatctgc ttccacagac gagtacaagg acaggcaaaa ggaattggaa   1800
gaagttgcta acccaataat gagcaagttc tatggagctg ctggtggagc tcctggtgga   1860
gctcctggtg gcttccctgg aggtttccct ggcggagctg gcgcagctgg cggtgcccca   1920
ggtggtgctg ccccaggcgg agacagcgga ccaaccgtgg aagaagtcga ttaa         1974
<210>  51
<211>  2124
<212>  DNA
<213>  Pichia pastoris
<400>  51
atgagtgttc catttggagt agatctaggt aacaacaaca ctgtgatcgg tgttgcccgt     60
aacagaggta ttgatattct tgtcaatgaa gtctctaatc gtcagacccc cagcattgtc    120
ggatttggcg ctaagtctag agccatcggg gaatcaggaa agacccaaca gaactctaac    180
ttgaagaata ccgttgaaca tttggtccgt attctcgggc ttcctgcaga ctctcctgac    240
tatgaaattg agaagaagtt cttcacttcg cccctgattg agaaggacaa tgagatcctg    300
tctgaagtta acttccaagg taagaagact accttcacac ccattcagct ggttgccatg    360
tacctgaaca agattaagaa cactgccata aaggaaacaa agggaaagtt cactgatatc    420
tgtcttgctg tccctgtttg gttcaccgag aaacagagaa gtgctgcttc cgatgcttgt    480
aaggttgctg gtctgaaccc agttagaatt gtcaacgaca tcacagctgc tgcagttgga    540
tatggtgtct tcaagactga cctaccagag gatgaaccca agaaggttgc aatcgttgat    600
ataggccact ctacctattc tgttttgatt gctgctttca agaaaggtga gctgaaagtg    660
ttaggatctg cttctgacaa gcatttcggt ggtcgtgatt tcgactatgc catcaccaag    720
cactttgcag aggagttcaa gagcaaatac aagattgata tcactcaaaa tcctaaggct    780
tggtctcgtg tttacactgc tgccgaaagg ttgaagaagg ttttgtccgc taacactaca    840
gctccattca atgttgaatc tgttatgaac gacgttgatg tttcttcttc gctgactaga    900
gaggagttag aaaagctggt gcaaccatta ttagaccgtg ctcatattcc cgttgagcgt    960
gctctggcca tggcaggtct caaggctgaa gatgtggaca ctgttgaggt tgtcggaggt   1020
tgtactcgtg ttccaacctt gaaagctact ctatctgaag tctttggaaa gcccttatct   1080
ttcactttaa accaagatga ggcaattgct cgtggtgcag ctttcatctg tgcaatgcac   1140
tcccctacac ttagagttcg tccattcaag tttgaggacg ttaaccctta ctctgtgtca   1200
tattattggg acaaagatcc tgccgctgag gacgatgacc acttagaggt cttcccagtg   1260
ggtggttctt tcccatcaac taaggtgatc acactttacc gttcacaaga tttcaacatt   1320
gaagcccgct acacggacaa gaatgcactt ccagctggca ctcaggagtt cattggcagg   1380
tggagcatca agggtgttgt tgtcaatgaa ggtgaagata ctatccagac taagattaag   1440
ctgagaaatg atccatctgg tttccatatc gtcgaatctg cttacacagt cgagaagaag   1500
actattcaag agccaatcga ggatccagaa gctgatgaag atgcagaacc tcagtacagg   1560
acagttgaga agctcgtcaa aaagaacgac ttggagatta ctggacagac actccaccta   1620
ccagatgagc tattaaactc ttatcttgag acagaggctg ccttagaggt ccaagacaaa   1680
cttgttgcag acaccgagga gcgcaagaac gctctggagg agtacattta cgagcttaga   1740
ggtaagttgg aagaccagta caaggagttt gctagcgaac aggaaaaaac caagcttaca   1800
gctaagctag agaaagctga ggaatggctt tacgacgaag gttatgattc tactaaagct   1860
aagtacattg ctaaatacga agagcttgcc tccattggaa atgttatccg aggtcgttat   1920
cttgccaaag aggaggagaa gaaacaagct atccgtgaaa aggaagaatc taagaaggct   1980
tctgctatcg ctgaaaagat ggctgccgag cgtgcttctc gtgaagctgc tggttctaca   2040
aatgaacaag cccagaagaa tgaagaaaac accaaagatg ccgacggtga tgtttctatg   2100
aaccaagatg agctagatta aact                                          2124
<210>  52
<211>  37
<212>  DNA
<213>  Artificial
<220>
<223>  primer pBR322#FOR#NotI
<400>  52
aatagcggcc gcgcatctcg ggcagcgttg ggtcctg                              37
<210>  53
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  primer pBR322#BACK#NotI
<400>  53
gattgcggcc gcgacgtcag gtggcacttt tcggggaaat                           40
<210>  54
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer puc19ORI #1-SacI
<400>  54
gatcgagctc tgagcaaaag gccagcaaag                                      30
<210>  55
<211>  29
<212>  DNA
<213>  Artificial
<220>
<223>  primer puc19ORI #2-SacI
<400>  55
gaaagagctc ccgtagaaaa gatcaaagg                                       29
<210>  56
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer ampR #1 Hind III
<400>  56
gccgaagctt acaataaccc tgataaatgc                                      30
<210>  57
<211>  29
<212>  DNA
<213>  Artificial
<220>
<223>  primer ampR #2 Hind III
<400>  57
gccgaagctt aaatcaatct aaagtatat                                       29
<210>  58
<211>  42
<212>  DNA
<213>  Artificial
<220>
<223>  primer cyc1TT#neu#FOR#BamH1
<400>  58
caatggatcc ccttttcctt tgtcgatatc atgtaattag tt                        42
<210>  59
<211>  34
<212>  DNA
<213>  Artificial
<220>
<223>  primer cyc1TT #2-Age I
<400>  59
gtggaccggt agcttgcaaa ttaaagcctt cgag                                 34
<210>  60
<211>  43
<212>  DNA
<213>  Artificial
<220>
<223>  primer zeoR#neu##1#kpn1
<400>  60
gatcggtacc cacacaccat agcttcaaaa tgtttctact cct                       43
<210>  61
<211>  62
<212>  DNA
<213>  Artificial
<220>
<223>  primer TEF1#back:##1
<400>  61
tactatgccg atgattaatt gtcaacaccg cccttagatt agattgctat gctttctttc     60
ta                                                                    62
<210>  62
<211>  81
<212>  DNA
<213>  Artificial
<220>
<223>  primer TEF1#back:##2#Nco1
<400>  62
ttggccatgg tttagttcct caccttgtcg tattatacta tgccgatata ctatgccgat     60
gattaattgt caacaccgcc c                                               81
<210>  63
<211>  43
<212>  DNA
<213>  Artificial
<220>
<223>  primer Sh ble#FOR##1#Nco1
<400>  63
taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccg                       43
<210>  64
<211>  70
<212>  DNA
<213>  Artificial
<220>
<223>  primer Sh ble#back##2#aat1
<400>  64
tccgaggcct gggacccgtg ggccgccgtc ggacgtgtca gtcctgctcc tcggccacga     60
agtgcacgca                                                            70
<210>  65
<211>  62
<212>  DNA
<213>  Artificial
<220>
<223>  primer cyc1TT#FOR##1#aat1
<400>  65
tcccaggcct cggagatccg tccccctttt cctttgtcga tatcatgtaa ttagttatgt     60
ca                                                                    62
<210>  66
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer cyc1TT#neu#back#Kpn1
<400>  66
acatggtacc tgcaaattaa agccttcgag cgtcccaaaa ccttc                     45
<210>  67
<211>  29
<212>  DNA
<213>  Artificial
<220>
<223>  primer kanR #1-Kpn I
<400>  67
ccgaggtacc gacatggagg cccagaata                                       29
<210>  68
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer kanR #2-Kpn I
<400>  68
ccgaggtacc agtatagcga ccagcattca                                      30
<210>  69
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer 5#AOX TT #1 HindIII/NotI
<400>  69
gattaagctt gcggccgcag aggatgtcag aatgccattt gcctg                     45
<210>  70
<211>  48
<212>  DNA
<213>  Artificial
<220>
<223>  primer 5#AOX TT #2 AscI/BamHI
<400>  70
gattggatcc ggcgcgccga tactcgagaa ttatggctta atcaagtg                  48
<210>  71
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  primer 3#AOX TT #3 BamHI
<400>  71
gattggatcc tatgattgga agtatgggaa tggtgatacc                           40
<210>  72
<211>  46
<212>  DNA
<213>  Artificial
<220>
<223>  primer 3#AOX TT #4 NotI/EcoR I
<400>  72
taaagaattc gcggccgcag caacgttgtc actgaagttg gcatca                    46
<210>  73
<211>  44
<212>  DNA
<213>  Artificial
<220>
<223>  primer eGFP#1 Aar I/Sbf I
<400>  73
gatccacctg caggccatgg tgagcaaggg cgaggagctg ttca                      44
<210>  74
<211>  46
<212>  DNA
<213>  Artificial
<220>
<223>  primer eGFP#2 SfiI
<400>  74
ggatggccga ggcggcctta cttgtacagc tcgtccatgc cgagag                    46
<210>  75
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  primer Paox #1 Apa I
<400>  75
aaccgggccc tctaacatcc aaagacgaaa gg                                   32
<210>  76
<211>  36
<212>  DNA
<213>  Artificial
<220>
<223>  primer Paox #2 Sbf I
<400>  76
catggcctgc aggtgtcgtt tcgaataatt agttgt                               36
<210>  77
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pgap #1 Apa I
<400>  77
aaccgggccc agatcttttt tgtagaaatg t                                    31
<210>  78
<211>  48
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pgap #2 Sbf I
<400>  78
catggcctgc aggtgatagt tgttcaattg attgaaatag ggacaaat                  48
<210>  79
<211>  34
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pgnd1 #1 Apa I
<400>  79
tatcgggccc tatggtagaa tcatcaattg gaat                                 34
<210>  80
<211>  43
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pgnd1 #2 Sbf I
<400>  80
catggcctgc aggtgatttg tatcagtctt gtttcttttc ttt                       43
<210>  81
<211>  39
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pgpm1 #1 Apa I
<400>  81
tattgggccc gaaagaaggt ttatctgact gttgcgcac                            39
<210>  82
<211>  39
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pgpm1 #2 Sbf I
<400>  82
catggcctgc aggtgtgttt gtttgtgtaa ttgaaagtt                            39
<210>  83
<211>  35
<212>  DNA
<213>  Artificial
<220>
<223>  primer PHSP90 #1 Apa I
<400>  83
gactgggccc ttcaagatct tttgaggact agaga                                35
<210>  84
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer PHSP90 #2 Sbf I
<400>  84
catggcctgc aggtgattga tatttttcca aaattaaaaa gttaa                     45
<210>  85
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pkar2 #1 Apa I
<400>  85
atcagggccc actatcaaag ctatcaattg tggaaatgga cagca                     45
<210>  86
<211>  48
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pkar2 #2 Apa I
<400>  86
catggcctgc aggtgtcttg agtgttggaa ttgaaattaa ggaagaag                  48
<210>  87
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pmcm1 #1 Apa I
<400>  87
gtacgggccc acagctttgg cttgaacaat                                      30
<210>  88
<211>  42
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pmcm1 #2 Sbf I
<400>  88
catggcctgc aggtggctaa atgaatgcgg gttagtgttt ga                        42
<210>  89
<211>  37
<212>  DNA
<213>  Artificial
<220>
<223>  primer PPpet9 #1 Apa I
<400>  89
agtacgggcc ctagaaaatt caccactgtc ggaaagt                              37
<210>  90
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ppet9 #2 Sfi I
<400>  90
catggcctgc aggtggaagt cgacgaagaa gttagacttg ttgtt                     45
<210>  91
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prad2 #1 Apa I
<400>  91
gtaagggccc gtatagtttg cagacatagt aggagagttt                           40
<210>  92
<211>  44
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prad2 #2 Sbf I
<400>  92
cattgcctgc aggtgatcct tagcccaacc tgatggaaaa acgg                      44
<210>  93
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prps2 #1 Apa I
<400>  93
gtacgggccc tcctgagaac ggacagcagc                                      30
<210>  94
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prps2 #2 Sbf I
<400>  94
catggcctgc aggtgattaa ctacactgaa aaagtcggaa tgtac                     45
<210>  95
<211>  34
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prps31 #1 Apa I
<400>  95
gtacgggccc ttgtttatag cctataatcg caga                                 34
<210>  96
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prps31 #2 Sbf I
<400>  96
catggcctgc aggtgtttgg cttcgtcggc aatacgtgaa tgctt                     45
<210>  97
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pssa1#2 #1 Apa I
<400>  97
gtaagggccc gttgtatcca ttcactattt                                      30
<210>  98
<211>  43
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pssa1#2 #2 Sbf I
<400>  98
catggcctgc aggtgaatgt ttaactttgt ttaatttcta tgc                       43
<210>  99
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pthi1 #1 Apa I
<400>  99
gtaagggccc atcttttcag cttcatcgtc ag                                   32
<210>  100
<211>  42
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pthi1 #2 Sbf I
<400>  100
catggcctgc aggtggatga tttattgaag tttccaaagt tg                        42
<210>  101
<211>  33
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ptpi #2 Apa I
<400>  101
gtaagggccc ttcaacgaga cactcttccg tca                                  33
<210>  102
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ptpi #2 Sbf I
<400>  102
catggcctgc aggtgtgtgt ttgtgataga tcttgtatat                           40
<210>  103
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pubi4 #1 Apa I
<400>  103
agaagggccc agaagattac cataaattga ga                                   32
<210>  104
<211>  43
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pubi4 #2 Sbf I
<400>  104
catggcctgc aggtgaaagc gacaaacgtc acgtgaacaa aag                       43
<210>  105
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  primer Peno #1 Apa I
<400>  105
tatcgggccc aaagagtgag aggaaagtac ct                                   32
<210>  106
<211>  43
<212>  DNA
<213>  Artificial
<220>
<223>  primer Peno #2 Sbf I
<400>  106
catggcctgc aggtgtttta gatgtagatt gttataattg tgt                       43
<210>  107
<211>  34
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prsp7 #1 Apa I
<400>  107
tatcgggccc tttcatccag ctctttaacc ttat                                 34
<210>  108
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prsp7 #2 Sbf I
<400>  108
catggcctgc aggtgcttgt gatactgctg ttaccgtgtg agttt                     45
<210>  109
<211>  46
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prpl1 #1 Apa I
<400>  109
tatcgggccc ataagtccta gaacaccact tgttagtaaa accggt                    46
<210>  110
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer Prpl1 #2 Sbf I
<400>  110
catggcctgc aggtgtttct attaattcgt ctccctagca aaaag                     45
<210>  111
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ptkl #1 Apa I
<400>  111
tttagggccc gatatcgatt ccactgctca gagtcttttc                           40
<210>  112
<211>  43
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ptkl #2 Sbf I
<400>  112
catggcctgc aggtgtgtgt agagtggatg tagaatacaa gtc                       43
<210>  113
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ppis #1 Apa I
<400>  113
aaccgggccc tttttcctct tcgttgtgtg gtaaactcgg                           40
<210>  114
<211>  46
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ppis #2 Sbf I
<400>  114
tgatgcctgc aggtggacta tctagagaca agtaaatttc catgtt                    46
<210>  115
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pfet3 #1 Apa I
<400>  115
aaccgggccc tttcgtacca aatggaaaaa tcacgtacaa                           40
<210>  116
<211>  44
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pfet3 #2 Sbf I
<400>  116
taatgcctgc aggtgaaaac tagatcctct ttggaacagg ccgt                      44
<210>  117
<211>  36
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pftr1 # 1 Apa
<400>  117
aaccgggccc tcgagtaaca cactactaac ttttta                               36
<210>  118
<211>  42
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pftr1 # 2 Sbf I
<400>  118
taatgcctgc aggtgtttga aaagaactac aacgaccact ga                        42
<210>  119
<211>  47
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pnmt1 #1 Apa I
<400>  119
aaccgggccc taacatgata tcatgatgta cgtacaaact aggatct                   47
<210>  120
<211>  36
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pnmt1 #2 Sbf I
<400>  120
taatgcctgc aggtggattg gtgattttga tggtca                               36
<210>  121
<211>  35
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ppho8 #1 Apa I
<400>  121
attagggccc ggtataagta tagcacatgt tgacg                                35
<210>  122
<211>  45
<212>  DNA
<213>  Artificial
<220>
<223>  primer Ppho8 #2 Sbf I
<400>  122
taatgcctgc aggtgtgctt tgaaattgaa ggggagagga cgcta                     45
<210>  123
<211>  37
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pfet3pre #1 Apa I
<400>  123
agcagggccc ttgtggtcct atgaattaac catttaa                              37
<210>  124
<211>  48
<212>  DNA
<213>  Artificial
<220>
<223>  primer Pfet3pre #2 Aar I
<400>  124
ctagtcatgg cctgcaggtg tcgatggagt gttggcggca gtggttac                  48
<210>  125
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<400>  125
caggtagaaa attcaccact gtcggaaagt tgtctacttc cgtcggttgg aaatacgagt     60
ctgttgttga gaagttggag gagaagagaa aggctgagga agctgagtac caggagaaga    120
agagagctta cacccagaga ttagacgcag ctagtgccga gtttgcccaa accgaggagg    180
gaaagcagtt ggctgccttt ggttactaaa tagtaaagta gggtatcttc aagtaatagt    240
atactaacca tctgaaataa ccaccgtcct gtagtttttt ttcgatatcg aagagcctat    300
gctagtactg tggatttgcg ctccatccaa catctgtgcg caaactaaaa cttccgagac    360
tgacatctac catcgctaga ccctaagtaa aaccaatctc gcgtccgaac ttttaaattt    420
cagtccttaa aacttcagag cattggttgt agtttccgga tctgaggggt cgtattggag    480
tcaagagacg gagctgcctc cacagcgcga aacgtcaacc ccaacaccaa cctgaatttg    540
caatcaccat ggggacaagt ttcagcagtc aatgggcaat tcagacgttg atacggtacc    600
catttgctaa gctcaatgac gatccatcca acttcagaga aaggcctttc tctggtatgc    660
tctggtattc attcgtcttt tatcactctc gttgcacaat gcccgggtac tcccggaaca    720
agggagtctt ccagccaagc tgtacagagt gaaaaataga aatacacctt tgcaatcaag    780
acgcgcgttg gccaatcaca agacttaatc ggtgcaaaga aggattacca aatttttttt    840
tcccaaaatc gctatataga aataatggag gaaaaagggt taatataaag gagaattccc    900
ccgtttttct ccccttgtct tttcttcttc aggctttctt acaaatctat aatattccaa    960
aatggctgac aacaacaagt ctaacttctt cgtcgacttc atg                     1003
<210>  126
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  GND1 promoter
<400>  126
tatggtagaa tcatcaattg gaatgaccct atcgttgcta tacagactcg tagccccatt     60
tcttgtttgt tggttttcgt cgtcaattgc ctccaaaatt gaagagatgg cacttcgtcg    120
cgtgggttcc tgggaaatat tttgaagtgg aacatcgttg aaataaggca gtatatcatg    180
ctgattctca gcattggagt cagtagcttc aagtgaatct atactgtatg agtcggaaga    240
ggatttccgg ctcgtttttg tcttcatttt gttattagaa ggaatgataa cgttgggaaa    300
ccggaggttg gagattttgt atatataaaa ctttcttgga gcttattaat aaatgcggga    360
tgcagtaaac ttgcatatat ctattgtaac acttttgcaa tagctgcatg ccttgactca    420
tcattcagta tcgtgtgaaa accaatgata catccgtaca ttcaaactac aaccttcctc    480
attagtaatt ctttttgaat ttttcggaac ccgaagctcc gcctatcccc ccaactaaca    540
catcttccaa tttgggtggg agaacaccta gcaacatcac gatcattgcg cgaacgttcg    600
cactgtattt ttttctccca aacacccaac ttctaggcca aatatccact tctcggggtt    660
ctattcaccc atttaattgt tggccttaaa agtcaattga gttccaatca tagtccctag    720
ttgattgctt gtagcaaatg ccacaacagt aggcatttac gtcctcacag tctcttccct    780
tgtccctcat tgatacctct ttattctccc ccaccaccat acactacctt cctcgcaccc    840
ctgtcatcac aaccgcaata taatcgatgc gcggtttctt gcctaatcca tcgtccaaca    900
gagaggtcgc tctccttata tatatagttg atcccccttt ttttctaccc ttgcaatttt    960
ttttttggga ccaaagaaaa gaaacaagac tgatacaaat atg                     1003
<210>  127
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  GPM1 promoter
<400>  127
gaaagaaggt ttatctgact gttgcgcacc accgaaacca aaagcgggtt tagctgcaga     60
actttcagtg gccggcttgt tgcccaaaga gactgttact gcctccatcg gtcttggtag    120
cgcctgaacc aaacgaaaaa gcaccactgg gattattgtt tcctccaaaa gacgcggtcg    180
tgtttgatgc tgttgaggcc gcaggcgctt gaccaaaact aaacgctttt gatgtattgt    240
ttgtaggggc tgttgcgttg ttagaagctg gctggttgtt cccaaaggaa aaagcacctg    300
ttgatccagt gttagatcca cctgcggcat tcccttgagc tgcggagcca aaaattgatc    360
cttggtttga gctctgtgta ccaaataaag atgaattggc cttagctccg tcagcaggag    420
cgtttgcttg tccaaagggg cctccagacg acgtttgcgt gttgttggca ttactagtat    480
tggcaggctg atttccaaac aatgagggct tggaggcgtt ctgggagtta ccaaatgaga    540
accctgtatt tccttggcca gagcctgaga aactgaaccc tgatccagac attatcaata    600
ccttgggtta ttagtagtgt ccgttatttt tctgtttagg ttacgatttt gccagatttt    660
ttgggaggag ggaaacaaaa gaaccagtgc tacacgacct ttaagtgcca tcaggcatcc    720
tgttttctcg acctcatctc atcacatccg tcagtctgag ctttcagttc tcagttttcg    780
attgactctt gccctgctgc gcgcacacca taccctggct ccctctcatg cttctggcgt    840
taccccggga atcgtacatc catgccgcga atcccggaca ggactcagac ggatttcact    900
attcctagtg ggcacaacct ccatatataa ggatacttct gccccctagc ttaagtgcct    960
ctattttgtc agtaacaact ttcaattaca caaacaaaca atg                     1003
<210>  128
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  HSP90 promoter
<400>  128
ttcaagatct tttgaggact agagagcaag acctgcattt gatagtcgaa gaggagaggc     60
aatttaaaga tctagtattg gagaaggaca gactgctcga tgttcaagat aagaatgctt    120
tgaatctaag gctacaacta ggttttgctt cccacaaggt aattgatcta aaccaattac    180
ttcctgtgca aaaaacactt ggagccacta ctagtctgga gttaatcaac tttcaaacag    240
acatcaaaac tttacacgtc gtaaacaatg tcatggacaa aatccgggtt aaggtcactg    300
gggagaagcg actcgtgaca ctgactcctg acagcaccaa caaggctcta gaatttccca    360
ctgagaatga taggccactg caagaacaac aaaatacaga gcatgaaaac gaaaacggtg    420
ctcaagatcc catagactca aaccaaacga atgattctca gacatactcc acacctagcc    480
gtatccatta tacgactaac gaggagagtt cctctaagaa ggttaaaata tcaaattgag    540
catgatacga tagcccgctg gcaaggaatt aagtgacact agatcgcaaa taattcggaa    600
tcactttatg tggaatcatc attcttgata aattgtgata aagtatcgtt cgcgagatgg    660
tttttccaga aatttcttgt ggatcaatat ctttaaacga tagtaaacga tgttgacagc    720
tccaacaatg tcggaactgc tcatcaaaga cggacaattt ccctattagc agaaaaatag    780
cccatttatc atcaccgcag cgctattact gaatgatttt tttgaatttt tccttgtctg    840
tgaagccgca aaccacattt tctggcgcgg ttacccggtc atagaagctg gtggaatatt    900
ccatgctacc tcgacgatga gcttgcgaaa tatatttaag tgtaagaact ccgcgtcatt    960
aagactctgg ttaacttttt aattttggaa aaatatcaat atg                     1003
<210>  129
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  KAR2 promoter
<400>  129
ccactatcaa agctatcaat tgtggaaatg gacagcaaaa cggaaacgca gtcaatgacg     60
atgatttcag agctaagcga ttgtgcgaaa ggtggttgga caatctgttt atgcttcttt    120
atgaagatct gagggtgtat acgatgtatc gtgccgaaca catgcacttg acggcacagc    180
aaatggaatt caagaagacc actttagaat gggagttaat agggatggtt tcatggaggt    240
taaaacactt caaggaggca tctgaagcat tcaagtatgc actaggtctg aggttttcgg    300
tcaaggcatg caagaaatta attgaattct atctgaacga acgctccaga atgaaccagc    360
cagaaacctc aattgccctc aacaacttaa atcaatccac attatccatc caagagattc    420
tcaagtatcg ttcgttcctc gatatcaacc taatttcaaa cttggtcaaa ctaggagttt    480
ggaatcaccg ctggtatgct gagttttctc caaaactcat agaaagcctt gcggttgttg    540
tggagaacgg agggcttatc aaggtagaaa acgaggttaa ggctacctat ttcgattcac    600
aagatggagt ttacgacttg atgaacgagg tattcaagtt catgaagcat tacgattatc    660
ctgggactga caactaagag ctcctagtga agacttgaga tggacatgat aaacaattat    720
agtgaaaata gaaaccataa tacaatattc taatagagga accgtttacc tgtggttcct    780
attgtggcct actgttacta gctagtgtaa tacacccttg cctcagcttt gcaagttgac    840
aactcagcca aatgatcttt gaatgcgcga aacctcaagg tccatcgaat tttctcgaat    900
tttcagtgtt ttcatacagc gtgtcatctt ctttcgcgta cttatttaaa tcgtacccag    960
atcccttctt cttccttaat ttcaattcca acactcaaga atg                     1003
<210>  130
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  MCM1 promoter
<400>  130
acagctttgg cttgaacaat agtggttgga tgttacccgg tgcgacaagt gctgagttgc     60
ggtaatttac gatttcagcg tccaccagaa tgggaattcc gggtaacacg cataccaggg    120
gaagaatatc acaaagatca cagagattgg ataaaactgg accagaaact accaaataag    180
tagaccgtca tactcagctc ctacaccaag aggtacttca gccttttacc ggtttaaaaa    240
gccccccgaa atcgactaat taccgaggca ttatgtttac tactgatgga gatttcgaaa    300
tatccttccc gattagtcca acatctcgaa attaaactgc gcagcactat gccgaaagct    360
atataaacaa tcatttcccc aactggaaca tcttttttct cttttcttcg tgtatcatcc    420
tttggtcttt taatctttca gaaaagtttt cattaaaatg cttccagctg gtgttatttt    480
agtcttttgt ctacttttca ttatcggggt aattatcgca gttatcctgg gcatgaagtg    540
gtacaagaag agaaagaact gagcatggac gaagaaaatt actagaccaa agatgtatca    600
ccaaacaaac ccccccaaga atctgtcata aacgaagttg ttataggatg gttatcactc    660
tcacatttta atacaggaat accctaattt ttcctcagtt cggtgacgaa tggagttgta    720
gttatctttc tttccctcgt ttctgctctt atttccccct ctcgtcgcat catttgcatc    780
aaattggtgc aacgtgcgcg cacgctcggc tttggctgca acgactttct gtcgtgtacc    840
gacccatatt ctatcgcttc gggtagtccg caacaccctc aacttcctaa ttttgtgttc    900
ttacaagctg caaaaaagta ggactcactc aataaggtaa gtccccaaac atcaatccca    960
attgggtaat ccatcaaaca ctaacccgca ttcatttagc atg                     1003
<210>  131
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  RAD2 promoter
<400>  131
atggtatagt ttgcagacat agtaggagag tttgcgggct tgcaactcta acaagtctct     60
gtagtcgctg agactaatgc aatcgtcaag actactgggc tcgttatccg aaaaagtccc    120
ggagtaacta gtcggtaact ctctgtaccg aatcatattc atcctggggt tgatctgatc    180
ttggtccatg tcatcattct ctccatttcc cgtagggtag ggcaatccca ccagccactt    240
gttaaccttt tctttatgcg gcatattggg agtctgcttg gggttgaagc tttctggaaa    300
ctcctttttc ttgatcagat cgctgtcact acagaaccca ggatgctgct gataagggga    360
agaaacatag cccatgccca ttgagttgaa attgaagggt tcctgtgctg cacctgcatt    420
caacggatat acatgtatag gatgatggat catgtggctg taagaggggc cagacgctga    480
gttatcacca ttaacattgt taggagatcc tgagccttga aagggcacat tgctagtctt    540
tcccgtagtc attacaaaca gcgggccata gttcccgttg tcacaaggat atgcaattcc    600
tgtaggggta tagatgtgtc tgttgaatgg ggtcatggcg ggattctaga aaagagacgg    660
cccgttagtt aaatccaccc accgattatt gaggtccaac ttacaatttg gttttgacgg    720
tcttccattg gaggatatgt tgttctgagt tggtggtagt tacgtttatt tctccttcct    780
ttttggtttg tgttagagca aggacacgga gcactgacta ccttcaaggc catttgttaa    840
gaatccacag atagacacta ccacatggga aatgttgtta gggaagagct gatgctgagg    900
ctcaacatcg cgcgactcct tttagtcagt ttccaagtac ggttcactgt agacatttct    960
ctttttagtt tccgtttttc catcaggttg ggctaaggat atg                     1003
<210>  132
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  RPS2 promoter
<400>  132
ctcctgagaa cggacagcag cgctggaggc ggcctcttta acggtggcgg cgaagtcaac     60
aagggtagtt ttgattttgc ctaacaactg ttgcaggtcc ttctcagtct ttgggtggct    120
cttcttgaac tcgtccgatg catccttgac gaccttctca gcgtcgtcgg caatctcgtc    180
gacaacttgc actgatccat gagggtcttg ggtttgttcg acttctggaa ctggttttgg    240
cttgccagcg gaaactcctt ctgggggtgt ggcagcttcg gcgtaggtac tatcagtgtt    300
aatatcgggg attggttgat gtgcgagacg aggcaggcgg gagatccgtc tgcctcgtcc    360
aactgcgata gctcttactt acctcatgat gcttgtaaaa aagttaactg aaagatggaa    420
atgggagggg gaaaagaatt gtggtcaaat ccacgtcttg cgataacctc atacatttgc    480
tgcatgattg ggggatcatc gcaatttcgg tccttgtgac acacgcgagc tttccgcctg    540
gggttagagc gcggagcaag catactatca agcaagaaaa aatggtatgg agaaacctaa    600
ttggttaaga tatatgaaaa ctacgacggc ttcatacgtg gtatctctgg ttgagctact    660
acgaaccatt ttcccccttc aaaccctttg gcccatgcca ttcatgcctt gcctctctct    720
caagcaacta agcaatcaag caatttcccg ccttgctgca catgactgtt cggaaatcgg    780
agacccaaac accacttgtt atctatgcac gtgattttta tccagggcaa taaatactca    840
cttttgcttc aaaacgcttg gggcgcgcga gcggcaggct gggaaaaaaa taatctcaga    900
cttttcaaaa gactctcctc tttaatcatt gaataccgtc gatcgaaaac caccaccatc    960
ggctttccac gtacattccg actttttcag tgtagttaat atg                     1003
<210>  133
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  RPS31 promoter
<400>  133
ttgtttatag cctataatcg cagactttgt taacccgtag gaataatcct acccactaga     60
attagatctt ccaaccatac atagcgtgta ctacaactta agctggtcct cttcttttat    120
cattcgcgcg gctctatctc atctacacct ccattgaact aatggaaagc aaatacgccg    180
aaaaatatca agatcgattg attcttggag atgatggcga tgaggacgaa ttgtttgacg    240
agctggaaaa agatattgag gatcaattct tggccaaata cagagcagag agaatccaac    300
agttaaaaca ggagattacc aagatcaagg accatagttc aaacatcaac ctcaatgacc    360
acggtaacat gaaaacaata gatactgatg acgaactatt gaaagaaact gttgatagcg    420
aacgtgttgt gatccatttc tttaacccat cgtttagcac ttgccgtatc atggatgaga    480
agctgtctat aatcagcacc aaacatattg gaacgcgttt tttcagaatt gaagcacata    540
gagctccatt tttagttgca aagcttggta tcaaagtgct tccatgtgtt gtattgtact    600
acaaaggatt agaaagggat agaattgtcg gatttgacag attaagtaat tctcagacca    660
attttgagct agaagcttta gaggagttac tcttagatag tggaattgtg gaacgaagaa    720
ctgtcgattt tagcaacctg agaaacaagg tccaaaacaa ggtggatcag tcaaaatcag    780
actcagaaag tgatctagat atgtgataga tggcggatgg caggttcatt ctagtgtttc    840
acgtgacaca cgtgagcgtt taagggcaca caccctgact gacgcgcgaa catctaatct    900
gttccgcatg aaaaaaaaaa actacctcga cgaaattctc ttctagacag tttttacatt    960
ggtaagaaag aagcattcac gtattgccga cgaagccaaa atg                     1003
<210>  134
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  SSA1 promoter
<400>  134
gggttgtatc cattcactat ttactctttg tttcatttct tgaattattt ggatactact     60
ctgctggcaa ctctaccagt ctcaaacgca gaccaggttc gcaatttgat tagaatgttc    120
gtgagctctt acaatgaaaa gtccatgtac cttgcggcta gttgtgaatt atttttagtt    180
ccttctttgt tgctatcctc tttgaagtcg attatattgc tggaatggta tagggctccc    240
ttttcattta tcaggcaatt aatcgtggta ttctccgtga tctcgtttct gagattaaga    300
tatcaacaga atgtttacat gaaacaatta gttgatagtt atgatttgaa gatcagtcaa    360
ctcttatacc atccccaact tcctcaagga ttcaggttgg gatatttacg atttaagagt    420
ctattaacaa gcacgctagg atacttagaa ttggaaaaaa agaccagata atgagattga    480
actcgaaatt taggatcacc catatgacga agaattcatt tagattattg aaggtgtttt    540
catgtttacc tccatgagac catttctgtc acagcaaata caggcaacgc ttttcaccag    600
agcttgttgg tacaactttt cagatgacgc caaattctca cgcgcctcac tttgtgcggc    660
gctaacaata ggccattttt ttgtacctcc cggatggttc agctcaatca ctcgattgag    720
aggtttttgt tccgcgattt ttgttcaccc cacacttttc tcgaaggttc tagcaatcaa    780
gataaacacc gcaaagagag ccgcaggaac catatgtggt accacaagtg gtcttaaaca    840
actctggtag aattcgatgg aattcgatgg aagccgatcg actccgatcg aattgaagca    900
attcgtatat ataaggagaa cctagttcca ccccttactc gaccattagt ttacaagact    960
aacttcacag aagcatagaa attaaacaaa gttaaacatt atg                     1003
<210>  135
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  THI3 promoter
<400>  135
atcttttcag cttcatcgtc agtgatattt ctcagcccac agaccaagtc aactttggaa     60
tctaacaacc ttgttcttac aatgttagaa ctcttaagtc gcatgccatg atcttcaagc    120
tgaattttgt gaaggaggtc aaaccccaca atggcatcta gttgtttaga atacatgcct    180
tcgacaagtg tttgagtgtc caaaatcaag agctcaaaat tattgaattt gtctgccaat    240
aacgccgtaa attgattagt gtccagccca ccaacaatag gagcacctat agttaatttt    300
tcagataaat ttaagttatc aaggtaaagg agctctaagt ttaccccttc caacagggtt    360
atttgagaac tcaataaatt gttgaattca aaaccaattg tctttgaatt ctccactgga    420
gcttccttgc tgaaattgat tttgatacca ttggcatcaa agagacccgt atgataactc    480
cataaaaagg ggagatgata ggccttaaat tcatcgttaa tctgcaaatt tattcctgac    540
atgtctttgt aaatagttat agttcagaaa ctggaattga gctcaaaaaa ctggaatcga    600
gcggatattt gaagattgat gccttactca tgaattgatt gataagagct ccgtgattca    660
ctctgtcaat gattacccct ctcctacccg atttgggact ttttcttcag tcttggggac    720
tttttttcat atgacttgac cttgctttcc caatagggaa ggactcaccc atggatgatt    780
aagtttggat tactcgttta ggaaatagta gccatgaatc aatttgaatc ataccatcat    840
gaaatagggt taggctgtaa atgcctcaaa aatggctctt gaggctggat ttttgggtat    900
tggaatgttg gtagcaattg gtataaaagg ccatttgtat ttcacttttt tgtccttcat    960
actttactct tctcaacttt ggaaacttca ataaatcatc atg                     1003
<210>  136
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  TPI1 promoter
<400>  136
ttcaacgaga cactcttccg tcagttccaa aaccataagt ttgccgatgt gttggtcctt     60
gtaacgcatg gaatttgggc cagggtattt ttgatgaaat ggttcagatg gtctgtggag    120
gagtttgaag gcttacgaaa tataccacat tgccagttta tacagatggt taagggtgaa    180
aatcaacgtt acaccttgac gaccccatta ttacgatggc gtgaaggaga tgaagaccgg    240
gtagaagaaa taagaaaagc ggtacagttt aggtccggag atctagggaa ggaggcctta    300
gcttatattg tagctgctga gagagaggca gctgctggaa gatctgaagg ccctatcacg    360
tatgatgatg gtgatgacca ttagagaacg cccagagatt gatagccagt tcttggacaa    420
caattcggaa ctttattcac ggtgcaaaca tgatttgtgt ggatagcttc aagtcagaca    480
tttcatctca tccccccttt tactgctgct aatcaccgtt agtccgacag ttactctaat    540
caatatttat tagtgtttta gttgcgcaaa actcgagcct cttttcctta tctcttgaca    600
cttcctggag tcgaagtttt tcagcgcaaa ttcactctac aatgtctacc gatactagac    660
cgcctatctt ccccctctaa atagcctatt ggaagggtgc aataaggtat ataaatctgg    720
cgcgattccc ccggactttt atgatccaca tcacctcatc ttactgccct cactctcttt    780
cctgatcctc ccaggtccac cgatttcctc actatcgtcg gatttctcct tccagcgccc    840
tagagaattc cgtaaccacc gcaaaaatag cagccccccc ctcacccatt tttttattta    900
aaagaacacc ttactggccc gttttcgttt ctcctttact acaattgatt tttaattttc    960
agtttttttt cattgatata caagatctat cacaaacaca atg                     1003
<210>  137
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  UBI4 promoter
<400>  137
agaagattac cataaattga gaactcaaat gaccaagcca ggagtcaaga agaattctca     60
aaagggatac gcaaatattg attcggatga cgaagagctg actgaggctc tcgatttcaa    120
attggcacca aggtcaacaa aaagtctaga cataacacgg tctggactac cgcaacaata    180
taattttgaa aatgttctta gtagcgacga agaagatgga ggcggagagt ttcagggtga    240
ttctgtatcc ccaattaaaa acagttttca ttcaagtcca aggaagcagc gatctctact    300
ccacgtcata gacgaaggaa gtccatctat tggcccaatt tctgttcaac tgaaaagaag    360
tttgacttct cccacaaaga gacacaagaa tgctcaggaa gtgataagcc aaccagaaag    420
catgacatcc tcaaacttgt tttcgcctac acattcctcc gcctcaacta catcaataat    480
atctcccaaa ccccaagcgg caatgatccc aagtcttaac ccaaataacc ctttttatac    540
tgatactaat agctaatatg taataatgat taataaatgc accactttaa tttctttatc    600
aagaatagtt ttactatttc tctttcgggc atatacttga cccgctccat atatttcacc    660
tttatcgtga cattatctgc tgcacgaccc ggaattactt taaacttcta agaatttcca    720
aaataggaat cgggatgccg tattttccag tctgctggac agaacttgtg ctctctaaaa    780
tgaattgtag tggtcctcaa aggctactgc tactcacagt ctttactact ccagattgat    840
ccattccttg gcccatgcac agtaaattag actactgatc tgatgttgta tacttttgag    900
ctgattgatt acagttaacc tggtcttatc taatctctcg tttcttttta tctctattcc    960
catttctgta ctcttttgtt cacgtgacgt ttgtcgcttt atg                     1003
<210>  138
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  ENO1 promoter
<400>  138
atgaaagagt gagaggaaag tacctgggca aaatcacaca attccaaacc atgctaaatg     60
agatttaaag aacaaacgat ggcaaaaggc aaccgttata aatgtgatct ttcttggcag    120
ttatctgtca atttttctaa ggaacagtga attcatcata ggagagatgt tatacgttac    180
ataatcatac atactgcatg tatctcacct actttacctc atcaactcta aaacagttct    240
agtcccaacc ccagattcct agtcatgaca caagtccgca ccggacagga ctcacaacca    300
gcaagagaag ctaacaaatt tacgccccgg taaaacattc tttaggggcc gttcaatggt    360
aattttcctc tcacccgttt aaacttacct ccgggcggta tcttcaataa cctctgttgt    420
ccccgggtat cattggaaac agtgagggac gttgaacaga agagaggatc accgtaaatt    480
tgccttgcaa ttggccctaa ccacggatgg ttaacttcaa gccatcacga cagcaattga    540
gtcggcgcat agctaccctc ctcttcttga ccccatgcat aggaccaacc ttaaccgatg    600
gaacaggttc ctccgctccg tcccctggta gtgtctctgc gcaagaaata gttaaggtat    660
gaagactgat ctctcgcacc cccctcacag tactgttatg gtgaattgac aaagccattg    720
gctagattga aacatgtaat tcatatgtaa tcttgttcaa ttaacgagct tcgtacagtc    780
tcaatctaga cgtctgataa tggcgtttgt gctcctaatc gatgagccat ctcatgtgac    840
gtctatacgc ttcgatggct tccgtcgcga atatagaacc acttgaaata tgctgcaaac    900
cacgatccac cctggtcctg aaaagatata aatacagcac atctagcagg cttttgtctt    960
cttggttgaa acacacaatt ataacaatct acatctaaaa atg                     1003
<210>  139
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  RPS7A promoter
<400>  139
cctatgtaag ccttctcagc atagtcaaca aagaaacctg tttcgacgac tcctaccagt     60
cgtatcagct tttcatccag ctctttaacc ttatttaacg gaatctctcc aaaatcagca    120
tcaatgacga agttaccatt atctgtgacc actggaccag ctttcgcctt tccagcatct    180
ctcagagtgg cgctgttggc tcctagtttg aaaagatctt tcaatacttt aacataactg    240
ttgggtacaa cctcaatggg aacgcctttg cgccagaaat gacccagtct ttctggagac    300
tgtttacgaa aatctgccac cacaacgaat ttacgagaca atgatgccac caacttttct    360
tgataaagcg ctgcccctcc tccctttatc aaattcaagt tggtgtcaat ttcatcagct    420
ccatcaaagg ctacgtctat ctcatcgaac tcctccacat ttcctaaacg aaggccattg    480
tccaagataa gttgctttga ttgaaagctg gtagggatac aaataaactt ttctttattt    540
tccaactgcc ccaagcgttc ggcgacatag acaacggtgg atccagaccc aactcctata    600
attctgtcag acttcgaaac gttttcattg actgctgcga aagcagccaa tttttttgac    660
ttctcaatta gttcctcaga cattagttgt ctaagatgct agaggcaaga cgtgaataag    720
tacccacaaa ggacggtcta atttccctta cattcatcaa taggctattt caagcatgaa    780
cttacataag cgtattgtgc attagatatt aacctcttag catcccaaca ccgtactggg    840
cttcgaccct aaccacatcc cctacatcac atgactgtaa ttttttttgt tactactctt    900
ctcttgggac tgttgtgcgc gaatccagtt tttcctccag agccttaaca atcttttttc    960
aattctgtcg aaactcacac ggtaacagca gtatcacaag atg                     1003
<210>  140
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  RPL1 promoter
<400>  140
cataagtcct agaacaccac ttgttagtaa aaccggtaaa attccccatt ttctcatatt     60
caacttacaa tgctcaaaag atgatctttg aaggccatcc cagtggtgtt agccatccta    120
tggaagggtt tgttagtaat tgctgatcca aaacctgaag actgaaaaaa aggctcgcac    180
atgtcactca ttaccacatc cacagggaat tgctgcttct gcttgttact tatctcgagg    240
ttatcgtcta cttcagcctg aaagtaggaa ggtccatccg ctattggttc aatttcttga    300
agctttctaa ggtctttgtc catgttcaac tggtaaaaat gagtctttat catctcatgc    360
gtggtcttac tcaagatgtt ggcctgcatt gaagacaccc cttttggagg ctcacaggct    420
aggatatcta tcccaagaat attgcctctg gaagtcactt tatcggctgc aacttgggac    480
catgcaccag gagcaaaccc caaatccacg acattttgcc ctggacggaa gatattgaac    540
ttctcatgga tttggagcag cttaaacgct gcccttgatt tcaatgcctt tgcgtgagct    600
tcctttgtga aggggtcaga tgattgacgt tccagccatt tcctagagga tgaactcttt    660
ccttttactt tcagggaaac aaacctagca aactgcctcc gtagcagcga cacagaagga    720
tgctgtatca tgtgatgaac gtagctaagc acaacctgtg tgacaattcc gtgtcgttta    780
tcttatcttg ctagtctaga tataaagtat cacgtgacaa atattatttt tgccccaaca    840
ccggcttgaa acttagggct cgcgccctcg tcgcgagaat tcttaccttt cgttaaattt    900
tttttcagtg ttccaacttt tcccttctct atccatcgtc gaatcaaacc tttacgggat    960
tacctacaac ctttttgcta gggagacgaa ttaatagaaa atg                     1003
<210>  141
<211>  1009
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  TKL1 promoter
<400>  141
gatatcgatt ccactgctca gagtcttttc atcaagctct aagaaaagtc cggggatgga     60
ggagccagcg caacagaaaa ctatgtcggg actaaaacca acttcctcta aaacacgctc    120
acactggtca tatttggaga tatctgctgc cacgtaagaa attgtgttgc ccttctcggt    180
cccgtgaata tcaatagcgt cctttactac ctgctttagt aaggattctg ttctagccac    240
aatcacaacg gaacatcctt ttccatacaa aagtttagca aactcggctc caacaccctg    300
ggatgcaccc gtaataattg cctttttgtt ggtgacatca aaattatcaa acattccagt    360
tccctctaca ctttgtatga gaatgatagc tgaaattgtg caccagatgt tagaagataa    420
ggtcgtgtca tgaactaata tcatgaattc cgagggtggc tcaacaacta ttcacgtgac    480
ttggacgttg gaagttgagg tggttggtgg atgttgcacg gagtatcatt tgtaagcatg    540
aaatcagtct aaaaaacttg cagaatagca gagcggttcg gaaattcatt caaaaccacc    600
tcctcagatt ggatctgccc tactctgttt agctctggga gattttctcg gtcgtgttct    660
ttcgctggtc tacccacgct ataggaatcg ctgtgaacgc taccttcttc ccaacttctc    720
ggtgactatt ataagccatt cccactttgt tttcaagcac caacaaccca cccccacctt    780
atctactcca tcttgggtgt ccccgcgcct gttgcaaagt ccgaaccata gaacccccga    840
cctttgtccc actaaccctc agacacccct cggaagtcag ggagaaacca ctccgaagta    900
cattaatcat ccctcgtatt ctcgacggtg cccattttct ttataaaaag ggagacacag    960
gttgcttcac taactctaga cttgtattct acatccactc tacacaatg               1009
<210>  142
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  PIS1 promoter
<400>  142
tttttcctct tcgttgtgtg gtaaactcgg taacgaggct aaaagttttt gcaaatttga     60
gtcttcattc aaatctaaga actctgaata aatctcaaac acttctttca tttcgatcaa    120
taagtgtagt agtttctgtc tataaatttc ttcggtgttt gttctttcta gagcgtattg    180
tatactaaca aagttggata cgaccaaagt ttggagctca ttgtccattt tgtgtaatcc    240
ttcatcgtca tcaagcagaa ttcttagctc cttttggatc ttggttgtaa aaggtctcaa    300
cgttgtagta ttgttcataa gaagcttctg taacacaaaa ttagctggct tggggcaaag    360
atacatcacg ctaacaagcg attgaataat tgacggaagc cttggtgtca gtatttctct    420
ggtaagtgca accttatgcc ttattttgtc cataataaaa tctagagtgg atatagctga    480
ttccaaacag gtcatctgtc gtacatcaat ggttgatgtg atgtaacact tcgactcaat    540
aatcttggtc aaagcactaa tatagttacc agcgtccgat gcaataatcc ttgggtgaag    600
gcaaaggacg tggacaagtt ttgtgccaaa ccaacggact tcagattgat ttgatctaag    660
ataatttgca gcacgagagg taacatgcgt caagtcggct tttgaagctt cattaacggc    720
gttctcatca gcaaggatgg ggagtatttc catcagatcc gcctcaactg aattatcctt    780
caaatgcgga atgacaacgt cgagtccaac aaatccttga tatcccatcc tggaagtcca    840
attgtatgat ctaaagagat tacttcattc tatttttttt ttttttttat tggaattttt    900
cagcccaagg ctctctctat cagtgtaccg accaatacgt atcagataca gataattttg    960
agctcaggca acatggaaat ttacttgtct ctagatagtc atg                     1003
<210>  143
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  FET3 promoter
<400>  143
tgggctttcg taccaaatgg aaaaatcacg tacaagtatg cccagagcta agctaatcgg     60
atggcaagta gaaagtggat gggtttcaca gaacaataaa gaatgagtga cggatattat    120
tggctggcag gcattaaaga tgcataattt aagctttctg ttttctactt ttggaattgt    180
cacaaatttg aactgtggat gttattgaaa cacagacccg tataaatacc tcttgagaga    240
aatttgaaag tgaagctatt tcagtgaatt aatcactcgc catacacgag gtagataatt    300
cacgtagacg aatttctttt gatccatttt attcagggtg gacagtcaga agtgttcgtt    360
cacctgatat gttctagatg cagctcgaaa cgctgtaaaa aaaaaaagtc ccaaaagtca    420
cgtgcataaa ggtgtagttc aatttaatgg agataacata acatctatga ctcctttcat    480
gaatccatct caaaaacaca aactttgcta gaatatctgg tggcaccgat ttttcatcat    540
ttcacgagtt tatatagcat atgcgccaac agaacgttgc ctgacacaat gttaaggctt    600
ttaaattttg cttgtgtagt aaaaagttag tagtgtgtta ctcgatatca tatttctatc    660
agaagtggaa tattctaatc tctcctctac ctttgttaca atccgtttcg aacagaaaaa    720
agaatttatg atgattttat ggagaatcat tccaataata gcattgctaa ttagattgac    780
ggtagcaaag actcacaagt tcaatttgac agcttcttgg gtgaaagcaa atccagatgg    840
tgtttttgag agagatgtca ttggacttaa cggacagtgg cctctaccag ttctaagggt    900
gaatcaagga gacaggatcg aactgttgtt gacgaatggt cttggcaatg cgaatacatc    960
tttgcatttc cacggcctgt tccaaagagg atctagtttt atg                     1003
<210>  144
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  FTR1 promoter
<400>  144
tcgagtaaca cactactaac tttttactac acaagcaaaa tttaaaagcc ttaacattgt     60
gtcaggcaac gttctgttgg cgcatatgct atataaactc gtgaaatgat gaaaaatcgg    120
tgccaccaga tattctagca aagtttgtgt ttttgagatg gattcatgaa aggagtcata    180
gatgttatgt tatctccatt aaattgaact acacctttat gcacgtgact tttgggactt    240
ttttttttta cagcgtttcg agctgcatct agaacatatc aggtgaacga acacttctga    300
ctgtccaccc tgaataaaat ggatcaaaag aaattcgtct acgtgaatta tctacctcgt    360
gtatggcgag tgattaattc actgaaatag cttcactttc aaatttctct caagaggtat    420
ttatacgggt ctgtgtttca ataacatcca cagttcaaat ttgtgacaat tccaaaagta    480
gaaaacagaa agcttaaatt atgcatcttt aatgcctgcc agccaataat atccgtcact    540
cattctttat tgttctgtga aacccatcca ctttctactt gccatccgat tagcttagct    600
ctgggcatac ttgtacgtga tttttccatt tggtacgaaa gcccacgaac cacacgactg    660
tacttgactt ttggggtcgt taatgtgcac agcccagaga tgatctgata attaatatca    720
tttcgcacca ctgtttaaaa aattcgataa tttgttacta aaatgctatt tttgatgcaa    780
tgcgtgcatg ttcatgcacc agtagataat aatcttaaat ttacaatata gaagctagtt    840
tcttaaagtt ttattggctt atgtgtttta gggagaaaag tttcagagct atataaactc    900
gatccttcct tccacaaatc tcgccttcaa gtcatttctc gaacttcctt ccaatagcag    960
tgccactcaa cgttcagtgg tcgttgtagt tcttttcaaa atg                     1003
<210>  145
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  NMT1 promoter
<400>  145
tcgacacaca gaggaaacga gggtaacatg atatcatgat gtacgtacaa actaggatct     60
ctggtcattc cacgggccac atcaggatcg aatttgtcgc cgtcctcttc ttcagcgcca    120
tcaggggctc cagcaccgtc gtcgtatacg taaacatcta aaaaggagat atcgtcttca    180
gttctggtgg aaaggatcat attgtctgtg gggtatactt gcaactcttg tttctcttct    240
aaaatttcac gttccgttgg aagagtgatg tatgcgtcac catctgcgtt gttctcatct    300
tcgtggtaac gggcacccgt acttgataac ccaggtagca tcgagacttt gaggccattg    360
gcaacagaat cctcatacgt ttcgtcatca tcgtaatgtt ctaaatcata ttcctttaga    420
tcatcatcaa tgtcatcttg agactgtagt ttgctagcta tagatttgtt gatgttggct    480
ttcatttctg aaatatcaga atcatctttc tgtgtttcgg ccagctcatc ctgggcatct    540
ttcagtttca aattggccat ggcttcaatt ctctccatct caacatcgtt catttcatat    600
ttctcgggaa actccgaggg gaagcctctg ggaacccatg ttgtagacga aatcataagt    660
gtacaatact gtctctttaa actgaaaaaa aaaatttgag aagaaaaaaa aaattgctgg    720
tcacatgata aggtggatcc cgtaatcaag cattaacctg gttcaggttc gtggattgtt    780
taggctttat aaaattgact ggtaggtccc cagtttcaag ttttctttag taatctactc    840
gcacaaatta ttgttgggca tctcgtccta tcagattcat atagatctat aattgattcc    900
cctcctgaaa cactttcaac caacatctga gggttctcga aaattcagta agattgactc    960
ccttctcatc caggcttggt gaccatcaaa atcaccaatc atg                     1003
<210>  146
<211>  1003
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  PHO8 promoter
<400>  146
ggtataagta tagcacatgt tgacgctgat ataatcatcc caaaaaagtg tgcggtttat     60
ttagaaagga gaagaggaac tcatgaaatt tttgaaggaa cgcacccatt ctaggtgatg    120
tacgggtgtc ttttgataag tagtggatag gttttattca ctggactata tggtactgta    180
caaaagaagt tcctcttcag atggtctgta gcttgcatgc aatgttctct ctaaggaatg    240
tgtatttgaa aggtcatgac ttaccataac cagtcgcaaa atgcttttgt tccaggttct    300
cttcctgagg aacgttttga cggggtatac ataccttatc gagatcaatt ttaggaatag    360
atactttctc tccaaccgtt aaaaatttgg aaatcttcac tttggcaggt tttaactcgt    420
caagagactt gacgtctagg atcttatact tgcttatgtc gttcttggtg tcctgatcga    480
gagagtcttc cctgacttgg tatttcttac cttcgtgctt aaaactatga gccgttttgt    540
ccttaatggg catgggaaga gttttcaatg tgcttaaatc caaatctttg ggagttttca    600
acagccatac ttctttcttt cccaaatcct tgaacggaga tatttctttg aggtctgtgc    660
gctgtttgaa attagcaggt ggttcaaaag tgaaatcctc tgaatcactg ctttcagagt    720
tgtcggagct aatatcggac tctgatataa actcccttgg atattctttt catttcagag    780
acaccctaat agttgtagga aagattaaac caagtgaaaa aaaaaattca atcgcgatgg    840
tagagtctgg aaggagatgt tctccttctg gctcacatct cgctaagtgg acattcttca    900
cggaactacg cacaaatcta tgcccctttc aggatctcgc gcaatctgca ttgatatgtg    960
atatttgttt tagcgtcctc tccccttcaa tttcaaagca atg                     1003
<210>  147
<211>  1006
<212>  DNA
<213>  Pichia pastoris
<220>
<221>  misc#feature
<223>  FET3pre promoter
<400>  147
ttgtggtcct atgaattaac catttaaaaa tattgctatt aatgattatg ttaggtggat     60
taatgttgtc attagtacat atattcgtat tttaacttaa ttactcatta attaagtgta    120
gaatgtcatt tcactggatt taacttgctt tgtaagtacg aaacaatgct cgatcaataa    180
tcaaacagtt tagttcagag attagattca aatataattc taaatttata gtatcagaaa    240
tatatattaa ggacagtgtt aattttacct catttaaact gctgctagcc attaaacgat    300
tgtgcttttt tagtccatgt ttaagaacaa atgaccattt gctaaaccta ctgtaatctt    360
attataaaaa tgaagcctac aaatcatttc ttacaagcaa atgattttac agccccttgt    420
gctgaatgta tagtacacat tggacgagga gaaacgatct aagtggccac tcgtaggtgt    480
taatgatact attcgctcga acttaacggt gcttaggatt ctacttaatc cttgcacgtg    540
acaaggctta aattaagctt gttttctgat gtgtgtcaaa aaaattttag cttgacatat    600
atcgaacgat acggcgtatt cttaggcaac aatttcaact tcttcgctta tttgcaatac    660
tggtatccac ggggtaatgc caaacttaac aaacagtaat ggatttgaag ctagcatgct    720
gtatttcaag gagcggagaa gcaaagatag ccccccttag attaaatgca tgtttaagct    780
ttcgaggcac ttttctgatt aatgcataag cctgcaggaa aggattccac ttatttagta    840
aaattatcag ttcaatcgcc aaataaaggc acaacaagaa aaagaaacaa aacaataaaa    900
tttcaacctt ctttcagcgt atataaaaga aatttactgc ccacttcctc gaagttttcc    960
tttcccctag ctgtaaccac tgccgccaac actccatcga agaatg                  1006