【手続補正書】
【提出日】平成22年2月16日(2010.2.16)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】００８７
【補正方法】変更
【補正の内容】
【００８７】
利点：
過去数十年において、結核は世界中で数百万人の死の原因となる世界的な健康への脅威として再出現している。いくつかの抗結核薬が既知であるにもかかわらず、病原性マイコバクテリア種からの１剤又は多剤耐性株の出現が、この恐ろしい疾病の復活の主な原因の１つとして広く考えられている。この状況を克服するために、新規の薬剤介入戦略を緊急に開発する必要がある。この目的を達するために、薬剤標的の同定は、最初の必要事項である。本明細書において、本発明は、抗菌化合物のスクリーニングのための標的の豊富な供給源であることが常に証明されている、マイコバクテリアのタンパク質合成全般に焦点を置く。真核生物の細胞質タンパク質の合成とは対照的に、ホルミル化／脱ホルミル化事象は、真正細菌において必須のステップであると考えられ、したがってＰＤＦ酵素の重要性は長い間予想されてきた。様々な細菌性ＰＤＦでの共有性にもかかわらず、マイコバクテリアＰＤＦはいくつかの独特の特徴を有する。それらの内、挿入（残基７４〜８５）配列（マイコバクテリア種のみに特異的）の酵素安定性及び該タンパク質の機能の維持における寄与は、重要な性質であり、今日まで他のいかなる細菌からも報告されていない。挿入配列に対して設計及び合成されたホスホチオレート修飾アンチセンスオリゴヌクレオチドは、培養中のマイコバクテリアの増殖及びマイコバクテリアペプチドデホルミラーゼ酵素の発現を妨げる。したがってこれらの結果は、それに基づく合理的薬剤設計が可能である、マイコバクテリア酵素の挿入領域の新規性を強調する。このため本発明は、マイコバクテリア酵素の該領域と相互作用し、かつマイコバクテリア増殖を阻害できる該酵素の発現又は産生に影響を与える任意の（生物的又は非生物的）抗マイコバクテリア化合物の同定／開発において明確に有利となる。
［配列表］
SEQUENCE LISTING
<210>  1
<211>  162
<212>  PRT
<213>  Staphylococcus aureus
<400>  1
Met Ala Ile Lys Lys Leu Val Pro Ala Ser His Pro Ile Leu Thr Lys 
1               5                   10                  15      
Lys Ala Gln Ala Val Lys Thr Phe Asp Asp Ser Leu Lys Arg Leu Leu 
20                  25                  30          
Gln Asp Leu Glu Asp Thr Met Tyr Ala Gln Glu Ala Ala Gly Leu Cys 
35                  40                  45              
Ala Pro Gln Ile Asn Gln Ser Leu Gln Val Ala Ile Ile Asp Met Glu 
50                  55                  60                  
Met Glu Gly Leu Leu Gln Leu Val Asn Pro Lys Ile Ile Ser Gln Ser 
65                  70                  75                  80  
Asn Glu Thr Ile Thr Asp Leu Glu Gly Ser Ile Thr Leu Pro Asp Val 
85                  90                  95      
Tyr Gly Glu Val Thr Arg Ser Lys Met Ile Val Val Glu Ser Tyr Asp 
100                 105                 110         
Val Asn Gly Asn Lys Val Glu Leu Thr Ala His Glu Asp Val Ala Arg 
115                 120                 125             
Met Ile Leu His Ile Ile Asp Gln Met Asn Gly Ile Pro Phe Thr Glu 
130                 135                 140                 
Arg Ala Asp Arg Ile Leu Thr Asp Lys Glu Val Glu Ala Tyr Phe Ile 
145                 150                 155                 160 
Asn Asp 
<210>  2
<211>  203
<212>  PRT
<213>  Streptococcus pneumoniae 
<400>  2
Met Ser Ala Ile Glu Arg Ile Thr Lys Ala Ala His Leu Ile Asp Met 
1               5                   10                  15      
Asn Asp Ile Ile Arg Glu Gly Asn Pro Thr Leu Arg Thr Val Ala Glu 
20                  25                  30          
Glu Val Thr Phe Pro Leu Ser Asp Gln Glu Ile Ile Leu Gly Glu Lys 
35                  40                  45              
Met Met Gln Phe Leu Lys His Ser Gln Asp Pro Val Met Ala Glu Lys 
50                  55                  60                  
Met Gly Leu Arg Gly Gly Val Gly Leu Ala Ala Pro Gln Leu Asp Ile 
65                  70                  75                  80  
Ser Lys Arg Ile Ile Ala Val Leu Val Pro Asn Ile Val Glu Glu Gly 
85                  90                  95      
Glu Thr Pro Gln Glu Ala Tyr Asp Leu Glu Ala Ile Met Tyr Asn Pro 
100                 105                 110         
Lys Ile Val Ser His Ser Val Gln Asp Ala Ala Leu Gly Glu Gly Glu 
115                 120                 125             
Gly Cys Leu Ser Val Asp Arg Asn Val Pro Gly Tyr Val Val Arg His 
130                 135                 140                 
Ala Arg Val Thr Val Asp Tyr Phe Asp Lys Asp Gly Glu Lys His Arg 
145                 150                 155                 160 
Ile Lys Leu Lys Gly Tyr Asn Ser Ile Val Val Gln His Glu Ile Asp 
165                 170                 175     
His Ile Asn Gly Ile Met Phe Tyr Asp Arg Ile Asn Glu Lys Asp Pro 
180                 185                 190         
Phe Ala Val Lys Asp Gly Leu Leu Ile Leu Glu 
195                 200             
<210>  3
<211>  169
<212>  PRT
<213>  Haemophillus influenzae
<400>  3
Met Thr Ala Leu Asn Val Leu Ile Tyr Pro Asp Asp His Leu Lys Val 
1               5                   10                  15      
Val Cys Glu Pro Val Thr Lys Val Asn Asp Ala Ile Arg Lys Ile Val 
20                  25                  30          
Asp Asp Met Phe Asp Thr Met Tyr Gln Glu Lys Gly Ile Gly Leu Ala 
35                  40                  45              
Ala Pro Gln Val Asp Ile Leu Gln Arg Ile Ile Thr Ile Asp Val Glu 
50                  55                  60                  
Gly Asp Lys Gln Asn Gln Phe Val Leu Ile Asn Pro Glu Ile Leu Ala 
65                  70                  75                  80  
Ser Glu Gly Glu Thr Gly Ile Glu Glu Gly Cys Leu Ser Ile Pro Gly 
85                  90                  95      
Phe Arg Ala Leu Val Pro Arg Lys Glu Lys Val Thr Val Arg Ala Leu 
100                 105                 110         
Asp Arg Asp Gly Lys Glu Phe Thr Leu Asp Ala Asp Gly Leu Leu Ala 
115                 120                 125             
Ile Cys Ile Gln His Glu Ile Asp His Leu Asn Gly Ile Leu Phe Val 
130                 135                 140                 
Asp Tyr Leu Ser Pro Leu Lys Arg Gln Arg Ile Lys Glu Lys Leu Ile 
145                 150                 155                 160 
Lys Tyr Lys Lys Gln Ile Ala Lys Ser 
165                 
<210>  4
<211>  178
<212>  PRT
<213>  Leptospira interrogans
<400>  4
Met Ser Val Arg Lys Ile Leu Arg Met Gly Asp Pro Ile Leu Arg Lys 
1               5                   10                  15      
Ile Ser Glu Pro Val Thr Glu Asp Glu Ile Gln Thr Lys Glu Phe Lys 
20                  25                  30          
Lys Leu Ile Arg Asp Met Phe Asp Thr Met Arg His Ala Glu Gly Val 
35                  40                  45              
Gly Leu Ala Ala Pro Gln Ile Gly Ile Leu Lys Gln Ile Val Val Val 
50                  55                  60                  
Gly Ser Glu Asp Asn Glu Arg Tyr Pro Gly Thr Pro Asp Val Pro Glu 
65                  70                  75                  80  
Arg Ile Ile Leu Asn Pro Val Ile Thr Pro Leu Thr Lys Asp Thr Ser 
85                  90                  95      
Gly Phe Trp Glu Gly Cys Leu Ser Val Pro Gly Met Arg Gly Tyr Val 
100                 105                 110         
Glu Arg Pro Asn Gln Ile Arg Met Gln Trp Met Asp Glu Lys Gly Asn 
115                 120                 125             
Gln Phe Asp Glu Thr Ile Asp Gly Tyr Lys Ala Ile Val Tyr Gln His 
130                 135                 140                 
Glu Cys Asp His Leu Gln Gly Ile Leu Tyr Val Asp Arg Leu Lys Asp 
145                 150                 155                 160 
Thr Lys Leu Phe Gly Phe Asn Glu Thr Leu Asp Ser Ser His Asn Val 
165                 170                 175     
Leu Asp 
<210>  5
<211>  187
<212>  PRT
<213>  Enterococcus faecelis
<400>  5
Met Ile Thr Met Lys Asp Ile Ile Arg Glu Gly Asn Pro Thr Leu Arg 
1               5                   10                  15      
Ala Val Ala Glu Glu Val Pro Val Pro Ile Thr Glu Glu Asp Arg Gln 
20                  25                  30          
Leu Gly Glu Asp Met Leu Thr Phe Leu Lys Asn Ser Gln Asp Pro Val 
35                  40                  45              
Lys Ala Glu Glu Leu Gln Leu Arg Gly Gly Val Gly Leu Ala Ala Pro 
50                  55                  60                  
Gln Leu Asp Ile Ser Lys Arg Ile Ile Ala Val His Val Pro Ser Asn 
65                  70                  75                  80  
Asp Pro Glu Asn Glu Thr Pro Ser Leu Ser Thr Val Met Tyr Asn Pro 
85                  90                  95      
Lys Ile Leu Ser His Ser Val Gln Asp Val Cys Leu Gly Glu Gly Glu 
100                 105                 110         
Gly Cys Leu Ser Val Asp Arg Asp Val Pro Gly Tyr Val Val Arg His 
115                 120                 125             
Asn Lys Ile Thr Val Ser Tyr Phe Asp Met Ala Gly Glu Lys His Lys 
130                 135                 140                 
Val Arg Leu Lys Asn Tyr Glu Ala Ile Val Val Gln His Glu Ile Asp 
145                 150                 155                 160 
His Ile Asn Gly Ile Met Phe Tyr Asp His Ile Asn Lys Glu Asn Pro 
165                 170                 175     
Phe Ala Leu Lys Glu Gly Val Leu Val Ile Glu 
180                 185         
<210>  6
<211>  174
<212>  PRT
<213>  Helicobacter pylori
<400>  6
Met Ala Leu Leu Glu Ile Ile His Tyr Pro Ser Lys Ile Leu Arg Thr 
1               5                   10                  15      
Ile Ser Lys Glu Val Val Ser Phe Asp Ala Lys Leu His Gln Gln Leu 
20                  25                  30          
Asp Asp Met Tyr Glu Thr Met Ile Ala Ser Glu Gly Ile Gly Leu Ala 
35                  40                  45              
Ala Ile Gln Val Gly Leu Pro Leu Arg Met Leu Ile Ile Asn Leu Pro 
50                  55                  60                  
Gln Glu Asp Gly Val Gln His Lys Glu Asp Cys Leu Glu Ile Ile Asn 
65                  70                  75                  80  
Pro Lys Phe Ile Glu Thr Gly Gly Ser Met Met Tyr Lys Glu Gly Cys 
85                  90                  95      
Leu Ser Val Pro Gly Phe Tyr Glu Glu Val Glu Arg Phe Glu Lys Val 
100                 105                 110         
Lys Ile Glu Tyr Gln Asn Arg Phe Ala Glu Val Lys Val Leu Glu Ala 
115                 120                 125             
Ser Glu Leu Leu Ala Val Ala Ile Gln His Glu Ile Asp His Leu Asn 
130                 135                 140                 
Gly Val Leu Phe Val Asp Lys Leu Ser Ile Leu Lys Arg Lys Lys Phe 
145                 150                 155                 160 
Glu Lys Glu Leu Lys Glu Leu Gln Lys Lys Gln Lys His Lys 
165                 170                 
<210>  7
<211>  160
<212>  PRT
<213>  Bacillus subtilis
<400>  7
Met Ala Val Lys Lys Val Val Thr His Pro Ala Glu Val Leu Glu Thr 
1               5                   10                  15      
Pro Ala Glu Thr Val Thr Val Phe Asp Lys Lys Leu Lys Lys Leu Leu 
20                  25                  30          
Asp Asp Met Tyr Asp Thr Met Leu Glu Met Asp Gly Val Gly Leu Ala 
35                  40                  45              
Ala Pro Gln Ile Gly Ile Leu Lys Arg Ala Ala Val Val Glu Ile Gly 
50                  55                  60                  
Asp Asp Arg Gly Arg Ile Asp Leu Val Asn Pro Glu Ile Leu Glu Lys 
65                  70                  75                  80  
Ser Gly Glu Gln Thr Gly Ile Glu Gly Cys Leu Ser Phe Pro Asn Val 
85                  90                  95      
Tyr Gly Asp Val Thr Arg Ala Asp Tyr Val Lys Val Arg Ala Phe Asn 
100                 105                 110         
Arg Gln Gly Lys Pro Phe Ile Leu Glu Ala Arg Gly Phe Leu Ala Arg 
115                 120                 125             
Ala Val Gln His Glu Met Asp His Leu Asp Gly Val Leu Phe Thr Ser 
130                 135                 140                 
Lys Ile Ser Lys Tyr Tyr Thr Glu Asp Glu Leu Ala Asp Met Glu Gly 
145                 150                 155                 160 
<210>  8
<211>  197
<212>  PRT
<213>  Mycobacterium tuberculosis
<400>  8
Met Ala Val Val Pro Ile Arg Ile Val Gly Asp Pro Val Leu His Thr 
1               5                   10                  15      
Ala Thr Thr Pro Val Thr Val Ala Ala Asp Gly Ser Leu Pro Ala Asp 
20                  25                  30          
Leu Ala Gln Leu Ile Ala Thr Met Tyr Asp Thr Met Asp Ala Ala Asn 
35                  40                  45              
Gly Val Gly Leu Ala Ala Asn Gln Ile Gly Cys Ser Leu Arg Leu Phe 
50                  55                  60                  
Val Tyr Asp Cys Ala Ala Asp Arg Ala Met Thr Ala Arg Arg Arg Gly 
65                  70                  75                  80  
Val Val Ile Asn Pro Val Leu Glu Thr Ser Glu Ile Pro Glu Thr Met 
85                  90                  95      
Pro Asp Pro Asp Thr Asp Asp Glu Gly Cys Leu Ser Val Pro Gly Glu 
100                 105                 110         
Ser Phe Pro Thr Gly Arg Ala Lys Trp Ala Arg Val Thr Gly Leu Asp 
115                 120                 125             
Ala Asp Gly Ser Pro Val Ser Ile Glu Gly Thr Gly Leu Phe Ala Arg 
130                 135                 140                 
Met Leu Gln His Glu Thr Gly His Leu Asp Gly Phe Leu Tyr Leu Asp 
145                 150                 155                 160 
Arg Leu Ile Gly Arg Tyr Ala Arg Asn Ala Lys Arg Ala Val Lys Ser 
165                 170                 175     
His Gly Trp Gly Val Pro Gly Leu Ser Trp Leu Pro Gly Glu Asp Pro 
180                 185                 190         
Asp Pro Phe Gly His 
195         
<210>  9
<211>  198
<212>  PRT
<213>  Mycobacterium smegmatis
<400>  9
Met Ala Val Val Pro Ile Arg Ile Val Gly Asp Pro Val Leu His Thr 
1               5                   10                  15      
Pro Thr Glu Pro Val Pro Val Gly Pro Asp Gly Ser Leu Pro Asp Asp 
20                  25                  30          
Leu Pro Ala Leu Ile Gln Asp Met Phe Asp Thr Met Asp Ala Ala Asn 
35                  40                  45              
Gly Val Gly Leu Ala Ala Asn Gln Ile Gly Val Ala Lys Arg Leu Phe 
50                  55                  60                  
Val Tyr Asp Cys Ala Pro Thr Arg Gly Gln Thr Thr Arg Arg Arg Gly 
65                  70                  75                  80  
Val Val Ile Asn Pro Val Leu Glu Thr Ser Glu Val Pro Glu Thr Met 
85                  90                  95      
Pro Asp Pro Asp Glu Asp Glu Glu Gly Cys Leu Ser Val Pro Gly Glu 
100                 105                 110         
Asn Phe Pro Thr Gly Arg Ala Asp Trp Ala Arg Val Thr Gly Leu Asp 
115                 120                 125             
Ala Asp Gly Ser Pro Ile Thr Leu Glu Gly Glu Asp Leu Phe Ala Arg 
130                 135                 140                 
Met Leu Gln His Glu Thr Gly His Leu Asp Gly Phe Leu Tyr Leu Asp 
145                 150                 155                 160 
Arg Leu Val Gly Arg Tyr Ala Arg Ala Ala Lys Lys Ala Val Lys Arg 
165                 170                 175     
Asn Gly Trp Gly Gly Val Pro Gly Leu Ser Trp Met Pro Gly Glu Val 
180                 185                 190         
Pro Asp Pro Phe Gly His 
195             
<210>  10
<211>  197
<212>  PRT
<213>  Mycobacterium bovis
<400>  10
Met Thr Val Val Pro Ile Arg Ile Val Gly Asp Pro Val Leu His Thr 
1               5                   10                  15      
Ala Thr Thr Pro Val Thr Val Ala Ala Asp Gly Ser Leu Pro Ala Asp 
20                  25                  30          
Leu Ala Gln Leu Ile Ala Thr Met Tyr Asp Thr Met Asp Ala Ala Asn 
35                  40                  45              
Gly Val Gly Leu Ala Ala Asn Gln Ile Gly Cys Ser Leu Arg Leu Phe 
50                  55                  60                  
Val Tyr Asp Cys Ala Ala Asp Arg Ala Met Thr Ala Arg Arg Arg Gly 
65                  70                  75                  80  
Val Val Ile Asn Pro Val Leu Glu Thr Ser Glu Ile Pro Glu Thr Met 
85                  90                  95      
Pro Asp Pro Asp Thr Asp Asp Glu Gly Cys Leu Ser Val Pro Gly Glu 
100                 105                 110         
Ser Phe Pro Thr Gly Arg Ala Lys Trp Ala Arg Val Thr Gly Leu Asp 
115                 120                 125             
Ala Asp Gly Ser Pro Val Ser Ile Glu Gly Thr Gly Leu Phe Ala Arg 
130                 135                 140                 
Met Leu Gln His Glu Thr Gly His Leu Asp Gly Phe Leu Tyr Leu Asp 
145                 150                 155                 160 
Arg Leu Ile Gly Arg Tyr Ala Arg Asn Ala Lys Arg Ala Val Lys Ser 
165                 170                 175     
His Gly Trp Gly Val Pro Gly Leu Ser Trp Leu Pro Gly Glu Asp Pro 
180                 185                 190         
Asp Pro Phe Gly His 
195         
<210>  11
<211>  197
<212>  PRT
<213>  Mycobacterium avium
<400>  11
Met Ala Val Val Pro Ile Arg Ile Val Gly Asp Pro Val Leu His Thr 
1               5                   10                  15      
Pro Thr Gln Pro Val Pro Val Gly Asp Asp Gly Ser Leu Pro Ala Asp 
20                  25                  30          
Leu Gly Lys Leu Ile Ala Asp Met Tyr Asp Thr Met Asp Ala Ala His 
35                  40                  45              
Gly Val Gly Leu Ala Ala Asn Gln Ile Gly Val Gly Leu Arg Val Phe 
50                  55                  60                  
Val Tyr Asp Cys Ala Asp Asp Arg Gly Leu Thr Glu Arg Arg Arg Gly 
65                  70                  75                  80  
Val Val Val Asn Pro Val Leu Glu Thr Ser Glu Ile Pro Glu Thr Met 
85                  90                  95      
Pro Asp Pro Asp Thr Asp Asp Glu Gly Cys Leu Ser Val Pro Gly Glu 
100                 105                 110         
Ser Phe Pro Thr Gly Arg Ala Ser Trp Ala Arg Val Thr Gly Leu Asp 
115                 120                 125             
Ala Asp Gly Asn Pro Val Ser Ile Glu Gly His Gly Leu Phe Ala Arg 
130                 135                 140                 
Met Leu Gln His Glu Thr Gly His Leu Asp Gly Phe Leu Tyr Leu Asp 
145                 150                 155                 160 
Arg Leu Ile Gly Arg Tyr Ala Arg Ser Ala Lys Arg Ala Val Lys Ser 
165                 170                 175     
His Asn Trp Gly Val Pro Gly Leu Ser Trp Met Pro Gly Glu Gly Pro 
180                 185                 190         
Asp Pro Phe Gly His 
195         
<210>  12
<211>  197
<212>  PRT
<213>  Mycobacterium leprae
<400>  12
Met Ala Ile Ala Pro Ile Arg Ile Val Gly Asp Pro Val Leu His Thr 
1               5                   10                  15      
Pro Thr Ala Pro Val Gln Val Ala Ala Asp Gly Ser Leu Pro Ala Asn 
20                  25                  30          
Leu Asn Gly Leu Ile Ser Thr Met Tyr Asp Thr Met Asp Ala Ala His 
35                  40                  45              
Gly Val Gly Leu Ala Ala Asn Gln Ile Gly Tyr Gly Leu Arg Val Phe 
50                  55                  60                  
Val Tyr Asp Cys Ala Glu Asp Cys Arg Gln Thr Ala Arg Arg Arg Gly 
65                  70                  75                  80  
Val Val Ile Asn Pro Ile Leu Glu Thr Ser Glu Ile Pro Glu Thr Met 
85                  90                  95      
Pro Asp Pro Asp Thr Asp Asn Glu Gly Cys Leu Ser Val Pro Gly Glu 
100                 105                 110         
Ser Phe Pro Ile Gly Arg Ala Gln Trp Ala Arg Val Thr Gly Leu Asp 
115                 120                 125             
Ala Asp Gly Asn Pro Val Thr Thr Glu Gly Thr Gly Leu Phe Ala Arg 
130                 135                 140                 
Met Leu Gln His Glu Thr Gly His Leu Asp Gly Phe Leu Tyr Leu Asp 
145                 150                 155                 160 
Tyr Leu Ile Gly Arg His Ala Arg Ser Ala Lys Arg Ala Ile Lys Ser 
165                 170                 175     
Arg His Trp Gly Val Pro Gly Leu Ser Trp Met Pro Gly Glu Val Pro 
180                 185                 190         
Asp Pro Phe Gly Pro 
195         
<210>  13
<211>  12
<212>  PRT
<213>  Mycobacterial
<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaaは自然界において生じる任意のアミノ酸であってよい
<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaaは自然界において生じる任意のアミノ酸であってよい
<400>  13
Xaa Thr Xaa Arg Arg Arg Gly Val Val Ile Asn Pro
1               5                   10
<210>  14
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  配列番号２１中のヒト結核菌デホルミラーゼ遺伝子配列に対するアンチセンスオリゴヌクレオチド
<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  ヒト結核菌デホルミラーゼ遺伝子配列に対する５’−３’アンチセンスオリゴヌクレオチド
<400>  14
gatgaccaca ccgcgtcggc gggcggtcat                                      30
<210>  15
<211>  18
<212>  DNA
<213>  Artificial
<220>
<223>  ヒト結核菌由来ゲノムＤＮＡ  ５’−３’デホルミラーゼオープンリーディングフレーム（５９４ｂｐ）
<400>  15
catatggcag tggtaccc                                                   18
<210>  16
<211>  19
<212>  DNA
<213>  Artificial
<220>
<223>  ヒト結核菌由来ゲノムＤＮＡ  ５’−３’デホルミラーゼオープンリーディングフレーム（５９４ｂｐ）
<400>  16
ccattagtga ccgaacggg                                                  19
<210>  17
<211>  25
<212>  DNA
<213>  Artificial
<220>
<223>  ヒト結核菌デホルミラーゼポリペプチドのアミノ酸７４〜８５を欠失しているデホルミラーゼタンパク質の変異のための外部プライマー
<400>  17
ggaattccat atggcagtcg taccc                                           25
<210>  18
<211>  24
<212>  DNA
<213>  Artificial
<220>
<223>  ヒト結核菌デホルミラーゼポリペプチドのアミノ酸７４〜８５を欠失しているデホルミラーゼタンパク質の変異のための外部プライマー
<400>  18
cccaagcttt tagtgaccga acgg                                            24
<210>  19
<211>  26
<212>  DNA
<213>  Artificial
<220>
<223>  ヒト結核菌デホルミラーゼポリペプチドのアミノ酸７４〜８５を欠失しているデホルミラーゼタンパク質の変異のための内部プライマー
<400>  19
gcggaccgcg cagtgcttga gacctc                                          26
<210>  20
<211>  25
<212>  DNA
<213>  Artificial
<220>
<223>  ヒト結核菌デホルミラーゼポリペプチドのアミノ酸７４〜８５を欠失しているデホルミラーゼタンパク質の変異のための内部プライマー
<400>  20
gaggtctcaa gcactgcgcg gtccg                                           25