【手続補正書】
【提出日】平成17年11月17日(2005.11.17)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０１３１
【補正方法】変更
【補正の内容】
【０１３１】 
【配列表】
<110> Tanabe Seiyaku Co., Ltd.
<120> Novel phosphodiesterase and gene thereof
<130> A00-4660
<140> JP H11-129343
<141> 1999-05-11
<150> JP H10-338861
<151> 1998-11-30
<160> 18
<210> 1
<211> 4576
<212> DNA
<213> human
<220> 
<221> CDS
<222> (251)..(2590)
<400> 1
gaattccggg cggcggcggc caaactccgc ggcgtcccca gggcgccacg ttcgccctcg            60
ccgccgcggc cgcgctgctc ttcggctccg acatggaaga tggaccttct aataatgcga           120
gctgcttccg aaggctgacc gagtgcttcc tgagccccaa agctaatggt atagatatag           180
aagtcatcca cagagatgtt acagttgaag agatgggggt agagaagact ttgaaggaaa           240
agaatgtaga atg agg ata gaa gag agg aaa tcc caa cat tta aca ggt ttg aca gat  298
Met Arg Ile Glu Glu Arg Lys Ser Gln His Leu Thr Gly Leu Thr Asp 
1               5                   10                 15 
gaa aaa gtg aag gca tat ctt tct ctt cac ccc cag gta tta gat gaa             346
Glu Lys Val Lys Ala Tyr Leu Ser Leu His Pro Gln Val Leu Asp Glu 
20                   25                  30 
ttt gta tct gaa agt gtt agt gca gag aca gta gag aaa tgg ctg aag             394
Phe Val Ser Glu Ser Val Ser Ala Glu Thr Val Glu Lys Trp Leu Lys 
35                  40                   45 
agg aag aac aac aaa tca gaa gat gaa tca gct cct aag gaa gtc agc             442
Arg Lys Asn Asn Lys Ser Glu Asp Glu Ser Ala Pro Lys Glu Val Ser 
50                  55                 60 
agg tac caa gat acg aat atg cag gga gtt gta tat gaa cta aac agc             490
Arg Tyr Gln Asp Thr Asn Met Gln Gly Val Val Tyr Glu Leu Asn Ser 
65                  70                  75                  80 
tat ata gaa caa cgg ttg gac aca gga gga gac aac cag cta ctc ctc             538
Tyr Ile Glu Gln Arg Leu Asp Thr Gly Gly Asp Asn Gln Leu Leu Leu 
85                  90                  95 
tat gaa ctg agc agc atc att aaa ata gcc aca aaa gcc gat gga ttt             586
Tyr Glu Leu Ser Ser Ile Ile Lys Ile Ala Thr Lys Ala Asp Gly Phe
100                 105                 110 
gca ctg tat ttc ctt gga gag tgc aat aat agc ctg tgt ata ttc acg             634
Ala Leu Tyr Phe Leu Gly Glu Cys Asn Asn Ser Leu Cys Ile Phe Thr 
115                 120                 125 
cca cct ggg ata aag gaa gga aaa ccc cgc ctc atc cct gct ggg ccc             682
Pro Pro Gly Ile Lys Glu Gly Lys Pro Arg Leu Ile Pro Ala Gly Pro
130                 135                 140 
atc act cag ggc acc acc gtc tct gct tat gtg gcc aag tcc agg aaa             730
Ile Thr Gln Gly Thr Thr Val Ser Ala Tyr Val Ala Lys Ser Arg Lys
145                 150                 155                 160 
aca ctg cta gta gaa gac atc ctt gga gat gaa cga ttt cca aga ggt             778
Thr Leu Leu Val Glu Asp Ile Leu Gly Asp Glu Arg Phe Pro Arg Gly
165                 170                 175 
act gga ctg gaa tca ggg act cgt atc cag tct gtt ctt tgc tta cca             826
Thr Gly Leu Glu Ser Gly Thr Arg Ile Gln Ser Val Leu Cys Leu Pro
180                 185                 190 
att gtc act gca att ggt gac ttg att ggt att ctc gag ctg tat cgg             874
Ile Val Thr Ala Ile Gly Asp Leu Ile Gly Ile Leu Glu Leu Tyr Arg
195                 200                 205 
cac tgg ggc aaa gaa gcc ttc tgt ctt agt cac cag gag gtt gca aca             922
His Trp Gly Lys Glu Ala Phe Cys Leu Ser His Gln Glu Val Ala Thr
210                 215                 220 
gca aat ctt gcc tgg gct tca gta gca ata cat cag gtg cag gta tgc             970
Ala Asn Leu Ala Trp Ala Ser Val Ala Ile His Gln Val Gln Val Cys
225                 230                 235                 240
aga ggc ctt gcc aaa cag aca gaa ttg aat gac ttc cta ctc gac gta             1018
Arg Gly Leu Ala Lys Gln Thr Glu Leu Asn Asp Phe Leu Leu Asp Val
245                 250                 255
tca aaa aca tat ttt gat aac ata gtt gca ata gat tct cta ctt gaa             1066
Ser Lys Thr Tyr Phe Asp Asn Ile Val Ala Ile Asp Ser Leu Leu Glu
260                 265                 270
cac ata atg ata tat gca aaa aac ctg gtg aat gcc gat cgt tgt gcg             1114
His Ile Met Ile Tyr Ala Lys Asn Leu Val Asn Ala Asp Arg Cys Ala
275                 280                 285
ctt ttc cag gtg gac cat aag aac aag gag tta tat tca gac ctt ttt             1162
Leu Phe Gln Val Asp His Lys Asn Lys Glu Leu Tyr Ser Asp Leu Phe
290                 295                 300
gat att gga gag gaa aag gaa gga aaa cct gtc ttc aag aag acc aaa             1210
Asp Ile Gly Glu Glu Lys Glu Gly Lys Pro Val Phe Lys Lys Thr Lys
305                 310                 315                 320
gag ata aga ttt tca att gag aaa gga att gct ggc caa gta gca aga             1258
Glu Ile Arg Phe Ser Ile Glu Lys Gly Ile Ala Gly Gln Val Ala Arg
325                 330                 335
aca ggg gaa gtc ctg aac att cca gat gcc tat gca gac cca cgc ttt             1306
Thr Gly Glu Val Leu Asn Ile Pro Asp Ala Tyr Ala Asp Pro Arg Phe
340                 345                 350
aac aga gaa gta gac ttg tac aca ggc tac acc acg cgg aac atc ctg             1354
Asn Arg Glu Val Asp Leu Tyr Thr Gly Tyr Thr Thr Arg Asn Ile Leu
355                 360                 365
tgc atg ccc atc gtc agc cga ggc agc gtg ata ggt gtg gtg cag atg             1402
Cys Met Pro Ile Val Ser Arg Gly Ser Val Ile Gly Val Val Gln Met
370                 375                 380
gtc aac aaa atc agt ggc agt gcc ttc tct aaa aca gat gaa aac aac             1450
Val Asn Lys Ile Ser Gly Ser Ala Phe Ser Lys Thr Asp Glu Asn Asn
385                 390                 395                 400
ttc aaa atg ttt gcc gtc ttt tgt gct tta gcc tta cac tgt gct aat             1498
Phe Lys Met Phe Ala Val Phe Cys Ala Leu Ala Leu His Cys Ala Asn
405                 410                 415
atg tat cat aga att cgc cac tca gag tgc att tac cgg gta acg atg             1546
Met Tyr His Arg Ile Arg His Ser Glu Cys Ile Tyr Arg Val Thr Met
420                 425                 430
gaa aag ctg tcc tac cat agc att tgt act tca gaa gag tgg caa ggt             1594
Glu Lys Leu Ser Tyr His Ser Ile Cys Thr Ser Glu Glu Trp Gln Gly
435                 440                 445
ctc atg caa ttc acc ctt ccc gtg cgt ctc tgc aaa gaa att gaa tta             1642
Leu Met Gln Phe Thr Leu Pro Val Arg Leu Cys Lys Glu Ile Glu Leu
450                 455                 460
ttc cac ttt gac att ggt cct ttt gaa aac atg tgg cct gga att ttt             1690
Phe His Phe Asp Ile Gly Pro Phe Glu Asn Met Trp Pro Gly Ile Phe
465                 470                 475                 480
gtc tac atg gtt cat cgg tcc tgt ggg aca tcc tgc ttt gag ctt gaa             1738
Val Tyr Met Val His Arg Ser Cys Gly Thr Ser Cys Phe Glu Leu Glu
485                 490                 495
aag ttg tgt cgt ttt att atg tct gtg aag aag aac tat cgg cgg gtt             1786
Lys Leu Cys Arg Phe Ile Met Ser Val Lys Lys Asn Tyr Arg Arg Val
500                 505                 510
cct tat cac aac tgg aag cat gcg gtc act gta gca cac tgc atg tat             1834
Pro Tyr His Asn Trp Lys His Ala Val Thr Val Ala His Cys Met Tyr
515                 520                 525
gcc ata ctt cag aac aat cac acg ctt ttc aca gac ctt gag cgc aaa             1882
Ala Ile Leu Gln Asn Asn His Thr Leu Phe Thr Asp Leu Glu Arg Lys
530                 535                 540
gga ctg ctg att gcg tgt ctg tgt cat gac ctg gac cac agg ggc ttc             1930
Gly Leu Leu Ile Ala Cys Leu Cys His Asp Leu Asp His Arg Gly Phe
545                 550                 555                 560
agt aac agc tac ctg cag aag ttc gac cac cct ctg gcc gct ctc tac             1978
Ser Asn Ser Tyr Leu Gln Lys Phe Asp His Pro Leu Ala Ala Leu Tyr
565                 570                 575
tcc act tcc acc atg gag cag cac cac ttc tcc cag act gtg tcc atc             2026
Ser Thr Ser Thr Met Glu Gln His His Phe Ser Gln Thr Val Ser Ile
580                 585                 590
ctt cag ttg gaa ggg cac aat atc ttc tcc act ctg agc tcc agt gaa             2074
Leu Gln Leu Glu Gly His Asn Ile Phe Ser Thr Leu Ser Ser Ser Glu
595                 600                 605
tat gag cag gtg ctt gag atc atc cgc aaa gcc atc att gcc aca gac             2122
Tyr Glu Gln Val Leu Glu Ile Ile Arg Lys Ala Ile Ile Ala Thr Asp
610                 615                 620
ctt gct tta tac ttt gga aac agg aag cag ttg gaa gag atg tac cag             2170
Leu Ala Leu Tyr Phe Gly Asn Arg Lys Gln Leu Glu Glu Met Tyr Gln
625                 630                 635                 640
acc gga tca cta aac ctt aat aat caa tca cat aga gac cgt gta att             2218
Thr Gly Ser Leu Asn Leu Asn Asn Gln Ser His Arg Asp Arg Val Ile
645                 650                 655
ggt ttg atg atg act gcc tgt gac ctt tgt tct gtg aca aaa ctg tgg             2266
Gly Leu Met Met Thr Ala Cys Asp Leu Cys Ser Val Thr Lys Leu Trp
660                 665                 670
ccc gtt aca aaa ttg acg gca aat gat ata tat gca gaa ttc tgg gct             2314
Pro Val Thr Lys Leu Thr Ala Asn Asp Ile Tyr Ala Glu Phe Trp Ala
675                 680                 685
gag ggt gat gaa atg aag aaa ttg gga ata cag cct att cct atg atg             2362
Glu Gly Asp Glu Met Lys Lys Leu Gly Ile Gln Pro Ile Pro Met Met
690                 695                 700
gac aga gac aag aag gat gaa gtc ccc caa ggc cag ctt ggg ttc tac             2410
Asp Arg Asp Lys Lys Asp Glu Val Pro Gln Gly Gln Leu Gly Phe Tyr
705                 710                 715                 720
aat gcc gtg gcc att ccc tgc tat aca acc ctt acc cag atc ctc cct             2458
Asn Ala Val Ala Ile Pro Cys Tyr Thr Thr Leu Thr Gln Ile Leu Pro
725                 730                 735
ccc acg gag cct ctt ctg aaa gca tgc agg gat aat ctc agt cag tgg             2506
Pro Thr Glu Pro Leu Leu Lys Ala Cys Arg Asp Asn Leu Ser Gln Trp
740                 745                 750
gag aag gtg att cga ggg gag gag act gca acc tgg att tca tcc cca             2554
Glu Lys Val Ile Arg Gly Glu Glu Thr Ala Thr Trp Ile Ser Ser Pro
755                 760                 765
tcc gtg gct cag aag gca gct gca tct gaa gat tga gcactggtca ccctgacacg       2610
Ser Val Ala Gln Lys Ala Ala Ala Ser Glu Asp
770                 775
ctgtcccacc tacagatcct catcttgctt ctttgacatt cttttccttt ttttgggggg           2670
ggtgggggga acctgcacct ggtaactggg gtgcaaacct cttcaagaag gtaacatcaa           2730
ataaataagt caagcagagg acttcctgcc aatctcttct gtgaggcatc atagacactg           2790
agcaaccagg accaccccca cgttcagaaa tcagctggcc aagtgactcc atttgacttg           2850
caaaccagcc ttttctaata ggctaatatt gctgaggcct taaaggaaat ggacaaaaat           2910
tatccagaag gggtactttt ccattgtatc tttctaataa gggtttaaaa tggtactatt           2970
atggtattgt acttgggctt taacatcaat gttgctttga tgttgttgga tataaatagg           3030
aattttacac attactattg tgaatggtga atgttcatgt atgacctact tgtaattaac           3090
ttgagttgta gtccacagcc tcaggacaaa tgtcgttgag gttacagagt aagaaatgat           3150
ggcaaaacgt caaactctta tttcagagct tcatgaattt agttagacta aacataattc           3210
tttaagttca acctaaaggg ctgagatcaa taaatttaac actagacgaa gtagacttcc           3270
tgtctttttg agaagagatg aggtatatgt tacaataaat ctcagaactt caagtagcag           3330
ttcaaaagat gtcagttttt aaaattgttt ttgttgttgt cttggcagtt ttactgaacc           3390
ctttgcataa agaacaaaat aaaagctcgg cattgtaatt tttttaatgg acaagtctta           3450
tggatacgag gggtacattt ttcataatga ttcctttata ttttcacttt gtgtcatatg           3510
cagaatttta gactctcatt cacaatgaaa agtttatttt aaacattgtt taattaaaat           3570
accatacagt tctcttttaa acatcaaacc ataaaaagtg tattttgtaa ttttactctg           3630
acctgccgca gtcacctctc acttatctct tccacgtact gcacggtcgt atttcatgag           3690
ctttctgtcc atagcacaga aacagagcag aaagtagtac aatcatgttg gaccttcttt           3750
ctgttctctt tactcttctc acagatcaga tcactccata gaagcctgtg ggtttcgatg           3810
gtttcttcta tacacctttt tggttgacca gtattactat acaatgtaag tgttttaaaa           3870
aatacgaaag taatactctg caccccttcc tacaaagatg ataaagcagt cacttctggc           3930
gcattttaat aatttaaaga tttttagtgc aatggcacgg taacctccaa acctgaatta           3990
gacagagact cactcaggaa gtgacaggcc catcatatca aataacttat tcacttttca           4050
tgtggcagga aactggaata tcgcttttaa taaaatggaa aaatatgctt ctacatattt           4110
accaccatag gcgttttgtt catatgagcc tggtttgtgc aaaattaaat cagaggcttc           4170
tacaacatgg tttatttatg ttgtagcaaa gttggctcta cataaacatt gttcttattt           4230
taaaattaac actatgtgtt cagttttctt gtgggcttct gaaagttgcc atcttccctc           4290
cgtggagctc catttgctat tttcattata cactatgagg taaaatgtaa taacaaaaga           4350
gagagaagta ccactgtggc tagatatata cacacacata tatatatgga tggatgtaat           4410
atatgtagca cacacacata gatgtatata ggatacacac tcatgtatgt aaacgtatac           4470
atatgtgtat atatgataca tacacataca cacacacgag agacagaagg aaagagagga           4530
agagagaagc aaacatgtag gaaaaaatat aaatcagccg gaattc                          4576
<210> 2
<211> 2406
<212> DNA
<213> human
<220> 
<221> CDS
<222> (15)..(2384)
<400> 2
tcttcggctc cgac atg gaa gat gga cct tct aat aat gcg agc tgc ttc cga agg       56
Met Glu Asp Gly Pro Ser Asn Asn Ala Ser Cys Phe Arg Arg
1               5                  10
ctg acc gag tgc ttc ctg agc ccc agt ttg aca gat gaa aaa gtg aag              104
Leu Thr Glu Cys Phe Leu Ser Pro Ser Leu Thr Asp Glu Lys Val Lys
15                  20                 25                  30
gca tat ctt tct ctt cac ccc cag gta tta gat gaa ttt gta tct gaa              152
Ala Tyr Leu Ser Leu His Pro Gln Val Leu Asp Glu Phe Val Ser Glu
35                 40                   45
agt gtt agt gca gag aca gta gag aaa tgg ctg aag agg aag aac aac              200
Ser Val Ser Ala Glu Thr Val Glu Lys Trp Leu Lys Arg Lys Asn Asn
50                   55                  60
aaa tca gaa gat gaa tca gct cct aag gaa gtc agc agg tac caa gat              248
Lys Ser Glu Asp Glu Ser Ala Pro Lys Glu Val Ser Arg Tyr Gln Asp
65                  70                  75
acg aat atg cag gga gtt gta tat gaa cta aac agc tat ata gaa caa              296
Thr Asn Met Gln Gly Val Val Tyr Glu Leu Asn Ser Tyr Ile Glu Gln
80                  85                 90
cgg ttg gac aca gga gga gac aac cag cta ctc ctc tat gaa ctg agc              344
Arg Leu Asp Thr Gly Gly Asp Asn Gln Leu Leu Leu Tyr Glu Leu Ser
95                 100                 105                 110
agc atc att aaa ata gcc aca aaa gcc gat gga ttt gca ctg tat ttc              392
Ser Ile Ile Lys Ile Ala Thr Lys Ala Asp Gly Phe Ala Leu Tyr Phe
115                 120                 125
ctt gga gag tgc aat aat agc ctg tgt ata ttc acg cca cct ggg ata              440
Leu Gly Glu Cys Asn Asn Ser Leu Cys Ile Phe Thr Pro Pro Gly Ile
130                 135                 140
aag gaa gga aaa ccc cgc ctc atc cct gct ggg ccc atc act cag ggc              488
Lys Glu Gly Lys Pro Arg Leu Ile Pro Ala Gly Pro Ile Thr Gln Gly
145                 150                 155
acc acc gtc tct gct tat gtg gcc aag tcc agg aaa aca ctg cta gta              536
Thr Thr Val Ser Ala Tyr Val Ala Lys Ser Arg Lys Thr Leu Leu Val
160                 165                 170
gaa gac atc ctt gga gat gaa cga ttt cca aga ggt act gga ctg gaa              584
Glu Asp Ile Leu Gly Asp Glu Arg Phe Pro Arg Gly Thr Gly Leu Glu
175                 180                 185                 190
tca ggg act cgt atc cag tct gtt ctt tgc tta cca att gtc act gca              632
Ser Gly Thr Arg Ile Gln Ser Val Leu Cys Leu Pro Ile Val Thr Ala
195                 200                 205
att ggt gac ttg att ggt att ctc gag ctg tat cgg cac tgg ggc aaa              680
Ile Gly Asp Leu Ile Gly Ile Leu Glu Leu Tyr Arg His Trp Gly Lys
210                 215                 220
GAA GCC TTC TGT CTT AGT CAC CAG GAG GTT GCA ACA GCA AAT CTT GCC              728
Glu Ala Phe Cys Leu Ser His Gln Glu Val Ala Thr Ala Asn Leu Ala
225                 230                 235
tgg gct tca gta gca ata cat cag gtg cag gta tgc aga ggc ctt gcc              776
Trp Ala Ser Val Ala Ile His Gln Val Gln Val Cys Arg Gly Leu Ala
240                 245                 250
aaa cag aca gaa ttg aat gac ttc cta ctc gac gta tca aaa aca tat              824
Lys Gln Thr Glu Leu Asn Asp Phe Leu Leu Asp Val Ser Lys Thr Tyr
255                 260                 265                 270
ttt gat aac ata gtt gca ata gat tct cta ctt gaa cac ata atg ata              872
Phe Asp Asn Ile Val Ala Ile Asp Ser Leu Leu Glu His Ile Met Ile
275                 280                 285
tat gca aaa aac ctg gtg aat gcc gat cgt tgt gcg ctt ttc cag gtg              920
Tyr Ala Lys Asn Leu Val Asn Ala Asp Arg Cys Ala Leu Phe Gln Val
290                 295                 300
gac cat aag aac aag gag tta tat tca gac ctt ttt gat att gga gag              968
Asp His Lys Asn Lys Glu Leu Tyr Ser Asp Leu Phe Asp Ile Gly Glu
305                 310                 315
gaa aag gaa gga aaa cct gtc ttc aag aag acc aaa gag ata aga ttt             1016
Glu Lys Glu Gly Lys Pro Val Phe Lys Lys Thr Lys Glu Ile Arg Phe
320                 325                 330
tca att gag aaa gga att gct ggc caa gta gca aga aca ggg gaa gtc             1064
Ser Ile Glu Lys Gly Ile Ala Gly Gln Val Ala Arg Thr Gly Glu Val
335                 340                 345                 350
ctg aac att cca gat gcc tat gca gac cca cgc ttt aac aga gaa gta             1112
Leu Asn Ile Pro Asp Ala Tyr Ala Asp Pro Arg Phe Asn Arg Glu Val
355                 360                 365
gac ttg tac aca ggc tac acc acg cgg aac atc ctg tgc atg ccc atc             1160
Asp Leu Tyr Thr Gly Tyr Thr Thr Arg Asn Ile Leu Cys Met Pro Ile
370                 375                 380
gtc agc cga ggc agc gtg ata ggt gtg gtg cag atg gtc aac aaa atc             1208
Val Ser Arg Gly Ser Val Ile Gly Val Val Gln Met Val Asn Lys Ile
385                 390                 395
agt ggc agt gcc ttc tct aaa aca gat gaa aac aac ttc aaa atg ttt             1256
Ser Gly Ser Ala Phe Ser Lys Thr Asp Glu Asn Asn Phe Lys Met Phe
400                 405                 410
GCC GTC ttt tgt gct tta gcc tta cac tgt gct aat atg tat cat aga             1304
Ala Val Phe Cys Ala Leu Ala Leu His Cys Ala Asn Met Tyr His Arg
415                 420                 425                 430
att cgc cac tca gag tgc att tac cgg gta acg atg gaa aag ctg tcc             1352
Ile Arg His Ser Glu Cys Ile Tyr Arg Val Thr Met Glu Lys Leu Ser
435                 440                 445
tac cat agc att tgt act tca gaa gag tgg caa ggt ctc atg caa ttc             1400
Tyr His Ser Ile Cys Thr Ser Glu Glu Trp Gln Gly Leu Met Gln Phe
450                 455                 460
acc ctt ccc gtg cgt ctc tgc aaa gaa att gaa tta ttc cac ttt gac             1448
Thr Leu Pro Val Arg Leu Cys Lys Glu Ile Glu Leu Phe His Phe Asp
465                 470                 475
att ggt cct ttt gaa aac atg tgg cct gga att ttt gtc tac atg gtt             1496
Ile Gly Pro Phe Glu Asn Met Trp Pro Gly Ile Phe Val Tyr Met Val
480                 485                 490
cat cgg tcc tgt ggg aca tcc tgc ttt gag ctt gaa aag ttg tgt cgt             1544
His Arg Ser Cys Gly Thr Ser Cys Phe Glu Leu Glu Lys Leu Cys Arg
495                 500                 505                 510
ttt att atg tct gtg aag aag aac tat cgg cgg gtt cct tat cac aac             1592
Phe Ile Met Ser Val Lys Lys Asn Tyr Arg Arg Val Pro Tyr His Asn
515                 520                 525
tgg aag cat gcg gtc act gta gca cac tgc atg tat gcc ata ctt cag             1640
Trp Lys His Ala Val Thr Val Ala His Cys Met Tyr Ala Ile Leu Gln
530                 535                 540
aac aat cac acg ctt ttc aca gac ctt gag cgc aaa gga ctg ctg att             1688
Asn Asn His Thr Leu Phe Thr Asp Leu Glu Arg Lys Gly Leu Leu Ile
545                 550                 555
gcg tgt ctg tgt cat gac ctg gac cac agg ggc ttc agt aac agc tac             1736
Ala Cys Leu Cys His Asp Leu Asp His Arg Gly Phe Ser Asn Ser Tyr
560                 565                 570
ctg cag aag ttc gac cac cct ctg gcc gct ctc tac tcc act tcc acc             1784
Leu Gln Lys Phe Asp His Pro Leu Ala Ala Leu Tyr Ser Thr Ser Thr
575                 580                 585                 590
atg gag cag cac cac ttc tcc cag act gtg tcc atc ctt cag ttg gaa             1832
Met Glu Gln His His Phe Ser Gln Thr Val Ser Ile Leu Gln Leu Glu
595                 600                 605
ggg cac aat atc ttc tcc act ctg agc tcc agt gaa tat gag cag gtg             1880
Gly His Asn Ile Phe Ser Thr Leu Ser Ser Ser Glu Tyr Glu Gln Val
610                 615                 620
ctt gag atc atc cgc aaa gcc atc att gcc aca gac ctt gct tta tac             1928
Leu Glu Ile Ile Arg Lys Ala Ile Ile Ala Thr Asp Leu Ala Leu Tyr
625                 630                 635
ttt gga aac agg aag cag ttg gaa gag atg tac cag acc gga tca cta             1976
Phe Gly Asn Arg Lys Gln Leu Glu Glu Met Tyr Gln Thr Gly Ser Leu
640                 645                 650
aac ctt aat aat caa tca cat aga gac cgt gta att ggt ttg atg atg             2024
Asn Leu Asn Asn Gln Ser His Arg Asp Arg Val Ile Gly Leu Met Met
655                 660                 665                 670
act gcc tgt gac ctt tgt tct gtg aca aaa ctg tgg ccc gtt aca aaa             2072
Thr Ala Cys Asp Leu Cys Ser Val Thr Lys Leu Trp Pro Val Thr Lys
675                 680                 685
ttg acg gca aat gat ata tat gca gaa ttc tgg gct gag ggt gat gaa             2120
Leu Thr Ala Asn Asp Ile Tyr Ala Glu Phe Trp Ala Glu Gly Asp Glu
690                 695                 700
atg aag aaa ttg gga ata cag cct att cct atg atg gac aga gac aag             2168
Met Lys Lys Leu Gly Ile Gln Pro Ile Pro Met Met Asp Arg Asp Lys
705                 710                 715
aag gat gaa gtc ccc caa ggc cag ctt ggg ttc tac aat gcc gtg gcc             2216
Lys Asp Glu Val Pro Gln Gly Gln Leu Gly Phe Tyr Asn Ala Val Ala
720                 725                 730
att ccc tgc tat aca acc ctt acc cag atc ctc cct ccc acg gag cct             2264
Ile Pro Cys Tyr Thr Thr Leu Thr Gln Ile Leu Pro Pro Thr Glu Pro
735                 740                 745                 750
ctt ctg aaa gca tgc agg gat aat ctc agt cag tgg gag aag gtg att             2312
Leu Leu Lys Ala Cys Arg Asp Asn Leu Ser Gln Trp Glu Lys Val Ile
755                 760                 765
cga ggg gag gag act gca acc tgg att tca tcc cca tcc gtg gct cag             2360
Arg Gly Glu Glu Thr Ala Thr Trp Ile Ser Ser Pro Ser Val Ala Gln
770                 775                 780
aag gca gct gca tct gaa gat tga gcactggtca ccctgacacg ct                    2406
Lys Ala Ala Ala Ser Glu Asp
785
<210> 3
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 3
aagctgtcct accatagc                                                          18
<210> 4
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 4
ggctgcggcc agagggtg                                                          18
<210> 5
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 5
actttcagaa gagtggcc                                                          18
<210> 6
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 6
tgcaggtaac tgttactg                                                          18
<210> 7
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 7
cgctgctctt cggctccg                                                          18
<210> 8
<211> 21
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 8
ggatctgtag gtgggacagc g                                                      21
<210> 9
<211> 31
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 9
gggaattcat gaggatagaa gagaggaaat c                                           31
<210> 10
<211> 22
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 10
agcgtgtcag ggtgaccagt gc                                                     22
<210> 11
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 11
cgctgctctt cggctccg                                                          18
<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 12
ggatctgtag gtgggacagc g                                                      21
<210> 13
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 13
tcttcggctc cgacatgg                                                          18
<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 14
agcgtgtcag ggtgaccagt gc                                                     22
<210> 15
<211> 3427
<212> DNA
<213> rat
<220> 
<221> CDS
<222> (281)..(2665)
<400> 15
gaattcggca cgaggcagcg gcgggcggcg gcggctcttc ctttcgcctg cgatccaagg            60
cttgctgctc ccagcccgct ccgggccccg gccacctcca ccgccgcggc tccccttaca           120
cccgggcgca caccgcgcgg actccttggg ttttccgggt gccggcgggg gctgccctgg           180
cctcggcccc ggctctgcgg ccggtggccg aactccgtgg cggccccgag gcaccgccct           240
cccccttgcc actgcctggc cgctgctctt cggctccgac atg gaa gat gga ccc tct         298
Met Glu Asp Gly Pro Ser
1              5
aac aat gcg agt tgc ttc cga agg ctg acc gag tgt ttc ctc agc ccc             346
Asn Asn Ala Ser Cys Phe Arg Arg Leu Thr Glu Cys Phe Leu Ser Pro
10                  15                  20
agt ttg acg gat gaa aag gtg aag gcc tat ctt tcc ctc cat ccc cag             394
Ser Leu Thr Asp Glu Lys Val Lys Ala Tyr Leu Ser Leu His Pro Gln
25                  30                  35
gta tta gac gag ttt gtt tct gaa agt gtt agt gcg gag act gtg gag             442
Val Leu Asp Glu Phe Val Ser Glu Ser Val Ser Ala Glu Thr Val Glu
40                  45                  50
aag tgg ctg aag agg aaa aac aac aaa gca gaa gat gaa cca tct cct             490
Lys Trp Leu Lys Arg Lys Asn Asn Lys Ala Glu Asp Glu Pro Ser Pro
55                   60                 65                   70
aag gaa gtc agc agg tac cag gac acg aac atg cag gga gtc gtg tac             538
Lys Glu Val Ser Arg Tyr Gln Asp Thr Asn Met Gln Gly Val Val Tyr
75                  80                  85
gag ctg aac agc tac ata gag cag cgc ctg gac acc ggc ggg gac aac             586
Glu Leu Asn Ser Tyr Ile Glu Gln Arg Leu Asp Thr Gly Gly Asp Asn
90                  95                 100
cac ctg ctc ctg tac gag cta agc agt atc atc agg ata gcc aca aaa             634
His Leu Leu Leu Tyr Glu Leu Ser Ser Ile Ile Arg Ile Ala Thr Lys
105                 110                 115
gcc gac gga ttt gca ctg tac ttc ctt gga gag tgc aat aat agt ctg             682
Ala Asp Gly Phe Ala Leu Tyr Phe Leu Gly Glu Cys Asn Asn Ser Leu
120                 125                 130      
tgt gtc ttc aca cca ccc gga atg aag gaa ggt caa ccc cgt ctc atc             730
Cys Val Phe Thr Pro Pro Gly Met Lys Glu Gly Gln Pro Arg Leu Ile
135                 140                 145                 150
ccc gca ggg ccc atc acc cag ggc acc acc atc tct gcc tat gtg gcc             778
Pro Ala Gly Pro Ile Thr Gln Gly Thr Thr Ile Ser Ala Tyr Val Ala
155                 160                 165
aag tct agg aag acc ctg ctg gta gag gac atc ctt ggg gat gag cga             826
Lys Ser Arg Lys Thr Leu Leu Val Glu Asp Ile Leu Gly Asp Glu Arg
170                 175                 180
ttt ccc aga ggc act ggt ctg gag tca gga acc cga atc cag tct gtc             874
Phe Pro Arg Gly Thr Gly Leu Glu Ser Gly Thr Arg Ile Gln Ser Val
185                 190                 195
ctt tgc ttg cct att gtc act gcc att gga gac ttg att ggc atc ctt             922
Leu Cys Leu Pro Ile Val Thr Ala Ile Gly Asp Leu Ile Gly Ile Leu
200                 205                 210
gaa ctg tac agg cac tgg ggc aaa gag gcc ttc tgc ctc agc cat cag             970
Glu Leu Tyr Arg His Trp Gly Lys Glu Ala Phe Cys Leu Ser His Gln
215                 220                 225                 230
gag gtt gca acc gcc aat ctc gct tgg gct tcc gta gca ata cac cag            1018
Glu Val Ala Thr Ala Asn Leu Ala Trp Ala Ser Val Ala Ile His Gln
235                 240                 245
gtg cag gtg tgc aga ggt ctc gcc aag cag acc gaa ctg aat gac ttc            1066
Val Gln Val Cys Arg Gly Leu Ala Lys Gln Thr Glu Leu Asn Asp Phe
250                 255                 260
ctg ctc gat gta tca aag aca tac ttt gat aac ata gtc gcc ata gac            1114
Leu Leu Asp Val Ser Lys Thr Tyr Phe Asp Asn Ile Val Ala Ile Asp
265                 270                 275
tct cta ctt gaa cac atc atg ata tat gca aaa aat cta gtg aac gcc            1162
Ser Leu Leu Glu His Ile Met Ile Tyr Ala Lys Asn Leu Val Asn Ala
280                 285                 290
gac cgc tgc gcg ctc ttc cag gtg gac cac aag aac aag gag ctg tac            1210
Asp Arg Cys Ala Leu Phe Gln Val Asp His Lys Asn Lys Glu Leu Tyr
295                 300                 305                 310
tcg gac ctg ttt gac att ggg gag gag aag gag ggg aag ccc gtc ttc            1258
Ser Asp Leu Phe Asp Ile Gly Glu Glu Lys Glu Gly Lys Pro Val Phe
315                 320                 325
aag aag acc aag gag atc aga ttt tcc att gag aaa ggg att gct ggt            1306
Lys Lys Thr Lys Glu Ile Arg Phe Ser Ile Glu Lys Gly Ile Ala Gly
330                 335                 340
caa gtg gca aga acg gga gaa gtc ctg aac att cct gat gcc tac gca             1354
Gln Val Ala Arg Thr Gly Glu Val Leu Asn Ile Pro Asp Ala Tyr Ala
345                 350                 355
gac ccg cgc ttt aac agg gag gtg gac ctg tac aca ggc tat acc acg             1402
Asp Pro Arg Phe Asn Arg Glu Val Asp Leu Tyr Thr Gly Tyr Thr Thr
360                 365                 370
cgg aac att ctg tgt atg ccc ata gtg agc cgc ggc agc gtg atc ggt             1450
Arg Asn Ile Leu Cys Met Pro Ile Val Ser Arg Gly Ser Val Ile Gly
375                 380                 385                 390
gtg gtg caa atg gtt aac aag atc agc ggc agc gcc ttc tcc aag acg             1498
Val Val Gln Met Val Asn Lys Ile Ser Gly Ser Ala Phe Ser Lys Thr
395                 400                 405
gat gag aac aac ttc aag atg ttt gct gtc ttc tgc gct ctg gcc ctg             1546
Asp Glu Asn Asn Phe Lys Met Phe Ala Val Phe Cys Ala Leu Ala Leu
410                 415                 420
cac tgc gct aac atg tac cac agg atc cgc cac tca gag tgc atc tac             1594
His Cys Ala Asn Met Tyr His Arg Ile Arg His Ser Glu Cys Ile Tyr
425                 430                 435
agg gtt acc atg gag aag ctg tct tac cac agc atc tgc acc tct gag             1642
Arg Val Thr Met Glu Lys Leu Ser Tyr His Ser Ile Cys Thr Ser Glu
440                 445                 450
gaa tgg caa ggc ctc atg cac ttc aac ttg cca gca cgc atc tgc cgg             1690
Glu Trp Gln Gly Leu Met His Phe Asn Leu Pro Ala Arg Ile Cys Arg
455                 460                 465                 470
gac atc gag cta ttc cac ttt gac att ggt cct ttc gag aac atg tgg             1738
Asp Ile Glu Leu Phe His Phe Asp Ile Gly Pro Phe Glu Asn Met Trp
475                 480                 485
cct ggg atc ttt gtc tac atg atc cat cgg tct tgt ggg aca tcc tgt             1786
Pro Gly Ile Phe Val Tyr Met Ile His Arg Ser Cys Gly Thr Ser Cys
490                 495                 500
ttt gaa ctt gaa aaa ttg tgc cgt ttt atc atg tct gtg aag aag aac             1834
Phe Glu Leu Glu Lys Leu Cys Arg Phe Ile Met Ser Val Lys Lys Asn
505                 510                 515
tat agg cgg gtt cct tac cac aac tgg aag cat gca gtc acg gtg gcg             1882
Tyr Arg Arg Val Pro Tyr His Asn Trp Lys His Ala Val Thr Val Ala
520                 525                 530
cac tgc atg tac gcc ata ctt caa aac aac aat ggc ctc ttc aca gac             1930
His Cys Met Tyr Ala Ile Leu Gln Asn Asn Asn Gly Leu Phe Thr Asp
535                 540                 545                 550
ctt gag cgc aaa ggc ctg cta att gcc tgt ctg tgc cat gac ctg gac             1978
Leu Glu Arg Lys Gly Leu Leu Ile Ala Cys Leu Cys His Asp Leu Asp
555                 560                 565
cac agg ggc ttc agt aac agc tac ctg cag aaa ttc gac cac ccc ctg             2026
His Arg Gly Phe Ser Asn Ser Tyr Leu Gln Lys Phe Asp His Pro Leu
570                 575                 580
gct gcg ttg tac tcc acc tcc acc atg gag caa cac cac ttc tcc cag             2074
Ala Ala Leu Tyr Ser Thr Ser Thr Met Glu Gln His His Phe Ser Gln
585                 590                 595
acg gtg tcc atc ctc cag ctg gaa gga cac aac atc ttc tcc acc ctg             2122
Thr Val Ser Ile Leu Gln Leu Glu Gly His Asn Ile Phe Ser Thr Leu
600                 605                 610
agc tcc agc gag tac gag cag gtg ctg gag atc atc cgc aaa gcc atc             2170
Ser Ser Ser Glu Tyr Glu Gln Val Leu Glu Ile Ile Arg Lys Ala Ile
615                 620                 625                 630
atc gcc act gac ctc gca ctg tac ttt ggg aac agg aag cag ttg gag             2218
Ile Ala Thr Asp Leu Ala Leu Tyr Phe Gly Asn Arg Lys Gln Leu Glu
635                 640                 645
gag atg tac cag aca ggg tcg ctg aac ctc cac aac cag tcc cat cga             2266
Glu Met Tyr Gln Thr Gly Ser Leu Asn Leu His Asn Gln Ser His Arg
650                 655                 660
gac cgc gtc atc ggc ttg atg atg act gcc tgc gat ctt tgc tct gtg             2314
Asp Arg Val Ile Gly Leu Met Met Thr Ala Cys Asp Leu Cys Ser Val
665                 670                 675
acg aaa cta tgg cca gtt aca aaa ttg aca gca aat gat ata tat gca             2362
Thr Lys Leu Trp Pro Val Thr Lys Leu Thr Ala Asn Asp Ile Tyr Ala
680                 685                 690
gag ttc tgg gct gag ggg gat gag atg aag aag ttg ggg ata cag ccc             2410
Glu Phe Trp Ala Glu Gly Asp Glu Met Lys Lys Leu Gly Ile Gln Pro
695                 700                 705                 710
atc cct atg atg gac aga gac aag cga gat gaa gtc cct caa gga cag             2458
Ile Pro Met Met Asp Arg Asp Lys Arg Asp Glu Val Pro Gln Gly Gln
715                 720                 725
ctt gga ttc tac aat gct gtg gcc atc ccc tgc tat acc acc ctg acg             2506
Leu Gly Phe Tyr Asn Ala Val Ala Ile Pro Cys Tyr Thr Thr Leu Thr
730                 735                 740
cag atc ctc cca ccc aca gag cct ctg ctg aag gcc tgc agg gat aac             2554
Gln Ile Leu Pro Pro Thr Glu Pro Leu Leu Lys Ala Cys Arg Asp Asn
745                 750                 755
ctc aat cag tgg gag aag gta att cga ggg gaa gag aca gca atg tgg             2602
Leu Asn Gln Trp Glu Lys Val Ile Arg Gly Glu Glu Thr Ala Met Trp
760                 765                 770
att tca ggc cca gca act agc aaa agc aca tct gag aag ccg acc agg             2650
Ile Ser Gly Pro Ala Thr Ser Lys Ser Thr Ser Glu Lys Pro Thr Arg
775                 780                 785                 790
aag gtc gat gac tga tcctgaggtg atgtctgcct agcaactgac tcaacctgct             2705
Lys Val Asp Asp  
tctgtgactt cgttcttttt atttttattt ttttaacggg gtgaaaacct ctctcagaag           2765
gtaccgtcgc atatccatgt gaagcagatg actccctgcg cacacctcgg accgtgagca           2825
acccgggctc caccgtgttc agacgtcggc tattccatgg ctccgcctga cccccgaatg           2885
ccatttgcta ccaggccaga actgcgctgg ctggaggggg cagagacgac aggaggggtt           2945
cttacctgca tccttccatg agggtgtggt tctgtgtttc atctctaaca gagatgctac           3005
tgcttggtgg cgtttgttag aaatgggaca catgcccctg tcgtgaagtt tacatgtgac           3065
cttcttgtag gtaacttgag ttcgtagcct gggacccctg taatgaaggt tacagtccac           3125
aggtgataga gaaattcaag ctgtaagtta caggtgcact acaagtgtgt tcattcagtt           3185
tacctggggg catggaggtg agtcagctcc acgaggaagg aagcatacct ctgccctcat           3245
caaggggaca cagggtacat cccaggcatc agagaactgc agctcacctc aaaccatgtc           3305
aaagaattaa aacacacccc catcccctca ctgtagcctt tggcaacttc gccaaaccct           3365
tcacacaaag aaaataaaag taaggcgtat aaatttcctc cagcaagcct cgtgccgaat           3425
tc                                                                          3427
<210> 16
<211> 3080
<212> DNA
<213> rat
<220> 
<221> CDS
<222> (634)..(3000)
<400> 16
gaattcggca cgagcttcag agcacacgca gccccccaac tccgccccta tgtcaagtgc            60
acgcgggcac acgggttgga cactcgccca cgcacgcaca cacacgcgcg tgcacataca           120
tgcacactcg tgcacacaca ctacttctag gtgtgtgggt ctaaagttgc ttccgtcgag           180
aggctgagct gagatggacc agtcttcatt ggtgcctaga aagctcctta cagctttcct           240
gaggatccca gaaaggaagg cgggagggag tagcatcgct ccttcttaga gttgtctccc           300
ccttttgcga gatggccagc gcagaaactc caagtacccg aagcccttgg aatactgctg           360
catggagcat attcgagcat cttggagagg tcctggactt gggccacccg ggtttggcaa           420
cttgttttga gcagccctgg atggagcccg gatccacctt tcccagagac ttgtccctac           480
ggccccactt tgaatttgtg aactggcaat gaagcagaaa ggagttttgt actggaggat           540
actttggcgg gctgcctccc gatcacattt aaaggtggga gcaaaggccc cgctctgctg           600
gcacttcgaa accgcacaga ctctcggggg cag atg agc aat gac tcc cca gaa ggt gcc    660
Met Ser Asn Asp Ser Pro Glu Gly Ala
1               5
gtg ggc tcc tgc aac gca aca ggt ttg acg gat gaa aag gtg aag gcc             708
Val Gly Ser Cys Asn Ala Thr Gly Leu Thr Asp Glu Lys Val Lys Ala
10                 15                   20                 25
tat ctt tcc ctc cat ccc cag gta tta gac gag ttt gtt tct gaa agt             756
Tyr Leu Ser Leu His Pro Gln Val Leu Asp Glu Phe Val Ser Glu Ser
30                  35                   40
gtt agt gcg gag act gtg gag aag tgg ctg aag agg aaa aac aac aaa             804
Val Ser Ala Glu Thr Val Glu Lys Trp Leu Lys Arg Lys Asn Asn Lys
45                  50                  55
gca gaa gat gaa cca tct cct aag gaa gtc agc agg tac cag gac acg             852
Ala Glu Asp Glu Pro Ser Pro Lys Glu Val Ser Arg Tyr Gln Asp Thr
60                  65                  70
aac atg cag gga gtc gtg tac gag ctg aac agc tac ata gag cag cgc             900
Asn Met Gln Gly Val Val Tyr Glu Leu Asn Ser Tyr Ile Glu Gln Arg
75                 80                  85
ctg gac acc ggc ggg gac aac cac ctg ctc ctg tac gag cta agc agt             948
Leu Asp Thr Gly Gly Asp Asn His Leu Leu Leu Tyr Glu Leu Ser Ser
90                 95                  100                 105
atc atc agg ata gcc aca aaa gcc gac gga ttt gca ctg tac ttc ctt             996
Ile Ile Arg Ile Ala Thr Lys Ala Asp Gly Phe Ala Leu Tyr Phe Leu
110                 115                 120
gga gag tgc aat aat agt ctg tgt gtc ttc aca cca ccc gga atg aag            1044
Gly Glu Cys Asn Asn Ser Leu Cys Val Phe Thr Pro Pro Gly Met Lys
125                 130                 135
gaa ggt caa ccc cgt ctc atc ccc gca ggg ccc atc acc cag ggc acc            1092
Glu Gly Gln Pro Arg Leu Ile Pro Ala Gly Pro Ile Thr Gln Gly Thr
140                 145                 150
acc atc tct gcc tat gtg gcc aag tct agg aag acc ctg ctg gta gag            1140
Thr Ile Ser Ala Tyr Val Ala Lys Ser Arg Lys Thr Leu Leu Val Glu
155                 160                 165
gac atc ctt ggg gat gag cga ttt ccc aga ggc act ggt ctg gag tca            1188
Asp Ile Leu Gly Asp Glu Arg Phe Pro Arg Gly Thr Gly Leu Glu Ser
170                 175                 180                 185
gga acc cga atc cag tct gtc ctt tgc ttg cct att gtc act gcc att            1236
Gly Thr Arg Ile Gln Ser Val Leu Cys Leu Pro Ile Val Thr Ala Ile
190                 195                 200
gga gac ttg att ggc atc ctt gaa ctg tac agg cac tgg ggc aaa gag            1284
Gly Asp Leu Ile Gly Ile Leu Glu Leu Tyr Arg His Trp Gly Lys Glu
205                 210                 215
gcc ttc tgc ctc agc cat cag gag gtt gca acc gcc aat ctc gct tgg            1332
Ala Phe Cys Leu Ser His Gln Glu Val Ala Thr Ala Asn Leu Ala Trp
220                 225                 230
gct tcc gta gca ata cac cag gtg cag gtg tgc aga ggt ctc gcc aag            1380
Ala Ser Val Ala Ile His Gln Val Gln Val Cys Arg Gly Leu Ala Lys
235                 240                 245
cag acc gaa ctg aat gac ttc ctg ctc gat gta tca aag aca tac ttt            1428
Gln Thr Glu Leu Asn Asp Phe Leu Leu Asp Val Ser Lys Thr Tyr Phe
250                 255                 260                 265
gat aac ata gtc gcc ata gac tct cta ctt gaa cac atc atg ata tat            1476
Asp Asn Ile Val Ala Ile Asp Ser Leu Leu Glu His Ile Met Ile Tyr
270                 275                 280
gca aaa aat cta gtg aac gcc gac cgc tgc gcg ctc ttc cag gtg gac            1524
Ala Lys Asn Leu Val Asn Ala Asp Arg Cys Ala Leu Phe Gln Val Asp
285                 290                 295
cac aag aac aag gag ctg tac tcg gac ctg ttt gac att ggg gag gag            1572
His Lys Asn Lys Glu Leu Tyr Ser Asp Leu Phe Asp Ile Gly Glu Glu
300                 305                 310
aag gag ggg aag ccc gtc ttc aag aag acc aag gag atc aga ttt tcc            1620
Lys Glu Gly Lys Pro Val Phe Lys Lys Thr Lys Glu Ile Arg Phe Ser
315                 320                 325
att gag aaa ggg att gct ggt caa gtg gca aga acg gga gaa gtc ctg            1668
Ile Glu Lys Gly Ile Ala Gly Gln Val Ala Arg Thr Gly Glu Val Leu
330                 335                 340                 345
aac att cct gat gcc tac gca gac ccg cgc ttt aac agg gag gtg gac            1716
Asn Ile Pro Asp Ala Tyr Ala Asp Pro Arg Phe Asn Arg Glu Val Asp
350                 355                 360
ctg tac aca ggc tat acc acg cgg aac att ctg tgt atg ccc ata gtg            1764
Leu Tyr Thr Gly Tyr Thr Thr Arg Asn Ile Leu Cys Met Pro Ile Val
365                 370                 375
agc cgc ggc agc gtg atc ggt gtg gtg caa atg gtt aac aag atc agc            1812
Ser Arg Gly Ser Val Ile Gly Val Val Gln Met Val Asn Lys Ile Ser
380                 385                 390
ggc agc gcc ttc tcc aag acg gat gag aac aac ttc aag atg ttt gct            1860
Gly Ser Ala Phe Ser Lys Thr Asp Glu Asn Asn Phe Lys Met Phe Ala
395                 400                 405
gtc ttc tgc gct ctg gcc ctg cac tgc gct aac atg tac cac agg atc            1908
Val Phe Cys Ala Leu Ala Leu His Cys Ala Asn Met Tyr His Arg Ile
410                 415                 420                 425
cgc cac tca gag tgc atc tac agg gtt acc atg gag aag ctg tct tac            1956
Arg His Ser Glu Cys Ile Tyr Arg Val Thr Met Glu Lys Leu Ser Tyr
430                 435                 440
cac agc atc tgc acc tct gag gaa tgg caa ggc ctc atg cac ttc aac            2004
His Ser Ile Cys Thr Ser Glu Glu Trp Gln Gly Leu Met His Phe Asn
445                 450                 455 
ttg cca gca cgc atc tgc cgg gac atc gag cta ttc cac ttt gac att            2052
Leu Pro Ala Arg Ile Cys Arg Asp Ile Glu Leu Phe His Phe Asp Ile
460                 465                 470
ggt cct ttc gag aac atg tgg cct ggg atc ttt gtc tac atg atc cat            2100
Gly Pro Phe Glu Asn Met Trp Pro Gly Ile Phe Val Tyr Met Ile His
475                 480                 485
cgg tct tgt ggg aca tcc tgt ttt gaa ctt gaa aaa ttg tgc cgt ttt            2148
Arg Ser Cys Gly Thr Ser Cys Phe Glu Leu Glu Lys Leu Cys Arg Phe
490                 495                 500                 505
atc atg tct gtg aag aag aac tat agg cgg gtt cct tac cac aac tgg            2196
Ile Met Ser Val Lys Lys Asn Tyr Arg Arg Val Pro Tyr His Asn Trp
510                 515                 520
aag cat gca gtc acg gtg gcg cac tgc atg tac gcc ata ctt caa aac            2244
Lys His Ala Val Thr Val Ala His Cys Met Tyr Ala Ile Leu Gln Asn
525                 530                 535
aac aat ggc ctc ttc aca gac ctt gag cgc aaa ggc ctg cta att gcc            2292
Asn Asn Gly Leu Phe Thr Asp Leu Glu Arg Lys Gly Leu Leu Ile Ala
540                 545                 550
tgt ctg tgc cat gac ctg gac cac agg ggc ttc agt aac agc tac ctg            2340
Cys Leu Cys His Asp Leu Asp His Arg Gly Phe Ser Asn Ser Tyr Leu
555                 560                 565
cag aaa ttc gac cac ccc ctg gct gcg ttg tac tcc acc tcc acc atg            2388
Gln Lys Phe Asp His Pro Leu Ala Ala Leu Tyr Ser Thr Ser Thr Met
570                 575                 580                 585
gag caa cac cac ttc tcc cag acg gtg tcc atc ctc cag ctg gag gga            2436
Glu Gln His His Phe Ser Gln Thr Val Ser Ile Leu Gln Leu Glu Gly
590                 595                 600
cac aac atc ttc tcc acc ctg agc tcc agc gag tac gag cag gtg ctg            2484
His Asn Ile Phe Ser Thr Leu Ser Ser Ser Glu Tyr Glu Gln Val Leu
605                 610                 615
gag atc atc cgc aaa gcc atc atc gcc act gac ctc gca ctg tac ttt            2532
Glu Ile Ile Arg Lys Ala Ile Ile Ala Thr Asp Leu Ala Leu Tyr Phe
620                 625                 630
ggg aac agg aag cag ttg gag gag atg tac cag aca ggg tcg ctg aac            2580
Gly Asn Arg Lys Gln Leu Glu Glu Met Tyr Gln Thr Gly Ser Leu Asn
635                 640                 645
ctc cac aac cag tcc cat cga gac cgc gtc atc ggc ttg atg atg act            2628
Leu His Asn Gln Ser His Arg Asp Arg Val Ile Gly Leu Met Met Thr
650                 655                 660                 665
gcc tgc gat ctt tgc tct gtg acg aaa cta tgg cca gtt aca aaa ttg            2676
Ala Cys Asp Leu Cys Ser Val Thr Lys Leu Trp Pro Val Thr Lys Leu
670                 675                 680
aca gca aat gat ata tat gca gag ttc tgg gct gag ggg gat gag atg            2724
Thr Ala Asn Asp Ile Tyr Ala Glu Phe Trp Ala Glu Gly Asp Glu Met
685                 690                 695
aag aag ttg ggg ata cag ccc atc cct atg atg gac aga gac aag cga            2772
Lys Lys Leu Gly Ile Gln Pro Ile Pro Met Met Asp Arg Asp Lys Arg
700                 705                 710
gat gaa gtc cct caa gga cag ctt gga ttc tac aat gct gtg gcc atc            2820
Asp Glu Val Pro Gln Gly Gln Leu Gly Phe Tyr Asn Ala Val Ala Ile
715                 720                 725
ccc tgc tat acc acc ctg acg cag atc ctc cca ccc aca gag cct ctg            2868
Pro Cys Tyr Thr Thr Leu Thr Gln Ile Leu Pro Pro Thr Glu Pro Leu
730                 735                 740                 745
ctg aag gcc tgc agg gat aac ctc aat cag tgg gag aag gta att cga            2916
Leu Lys Ala Cys Arg Asp Asn Leu Asn Gln Trp Glu Lys Val Ile Arg
750                 755                 760
ggg gaa gag aca gca atg tgg att tca ggc cca gca act agc aaa agc            2964
Gly Glu Glu Thr Ala Met Trp Ile Ser Gly Pro Ala Thr Ser Lys Ser
765                 770                 775
aca tct gag aag ccg acc agg aag gtc gat gac tga tcctgaggtg atgtctgcct      3020
Thr Ser Glu Lys Pro Thr Arg Lys Val Asp Asp
780                 785
agcaactgac tcaacctgct tctgtgactt cgttcttttt atttttattt ttttaacggg           3080
<210> 17
<211> 19
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 17
gcgctcttcc aggtggacc                                                          19
<210> 18
<211> 18
<212> DNA
<213> Artificial Sequence
<220> 
<223> synthetic primer
<400> 18
catcttgaag ttgttctc                                                            18
【手続補正２】
【補正対象書類名】明細書
【補正対象項目名】０１３２
【補正方法】削除
【補正の内容】
【手続補正３】
【補正対象書類名】明細書
【補正対象項目名】０１３３
【補正方法】削除
【補正の内容】
【手続補正４】
【補正対象書類名】明細書
【補正対象項目名】０１３４
【補正方法】削除
【補正の内容】
【手続補正５】
【補正対象書類名】明細書
【補正対象項目名】０１３５
【補正方法】削除
【補正の内容】
【手続補正６】
【補正対象書類名】明細書
【補正対象項目名】０１３６
【補正方法】削除
【補正の内容】
【手続補正７】
【補正対象書類名】明細書
【補正対象項目名】０１３７
【補正方法】削除
【補正の内容】
【手続補正８】
【補正対象書類名】明細書
【補正対象項目名】０１３８
【補正方法】削除
【補正の内容】
【手続補正９】
【補正対象書類名】明細書
【補正対象項目名】０１３９
【補正方法】削除
【補正の内容】
【手続補正１０】
【補正対象書類名】明細書
【補正対象項目名】０１４０
【補正方法】削除
【補正の内容】
【手続補正１１】
【補正対象書類名】明細書
【補正対象項目名】０１４１
【補正方法】削除
【補正の内容】
【手続補正１２】
【補正対象書類名】明細書
【補正対象項目名】０１４２
【補正方法】削除
【補正の内容】
【手続補正１３】
【補正対象書類名】明細書
【補正対象項目名】０１４３
【補正方法】削除
【補正の内容】
【手続補正１４】
【補正対象書類名】明細書
【補正対象項目名】０１４４
【補正方法】削除
【補正の内容】
【手続補正１５】
【補正対象書類名】明細書
【補正対象項目名】０１４５
【補正方法】削除
【補正の内容】
【手続補正１６】
【補正対象書類名】明細書
【補正対象項目名】０１４６
【補正方法】削除
【補正の内容】
【手続補正１７】
【補正対象書類名】明細書
【補正対象項目名】０１４７
【補正方法】削除
【補正の内容】
【手続補正１８】
【補正対象書類名】明細書
【補正対象項目名】０１４８
【補正方法】削除
【補正の内容】