【手続補正書】
【提出日】平成18年3月13日(2006.3.13)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】００１８
【補正方法】変更
【補正の内容】
【００１８】
前記ハイブリドーマを培養する培地としては、ハイブリドーマの培養に適した培地であればよく、好適にはダルベッコ氏変法イーグル氏最小必須培地（Ｄｕｌｂｅｃｃｏ’ｓ  ｍｏｄｉｆｉｅｄ  Ｅｅａｇｌｅ’ｓ  ｍｉｎｉｍｕｍ  ｅｓｓｅｎｔｉａｌ  ｍｅｄｉｕｍ）にウシ胎児血清、Ｌ−グルタミン、Ｌ−ピルビン酸、及び抗生物質（ペニシリンＧ及びストレプトマイシン）を含む培地が用いられる。
前記ハイブリドーマの培養は、培地中で行なう場合には、５％ＣＯ2濃度且つ３７℃の条件下で約３日間で行なうことができる。また、マウスの腹腔内で培養する場合には、約１４日間で行なうことができる。
【手続補正２】
【補正対象書類名】明細書
【補正対象項目名】００５１
【補正方法】変更
【補正の内容】
【００５１】
【配列表】
<110> Mitsubishi Kagaku Iatron, Inc.
<120> Novel Protein and Novel DNA Encoding the Same
<130> IAT99014P
<160> 2
<210> 1
<211> 1503
<212> DNA
<213> Homo sapiens
<400> 1
atg gcg gcg gcg tct gtc tct gcg gct tct ggt tct cac ttg tcg aac      48
Met Ala Ala Ala Ser Val Ser Ala Ala Ser Gly Ser His Leu Ser Asn
1               5                  10                  15
agc ttt gct gag cca tca agg tct aat gga agc atg gtt cgg cat tct      96
Ser Phe Ala Glu Pro Ser Arg Ser Asn Gly Ser Met Val Arg His Ser
20                  25                  30
tca tct cca tat gta gta tat cct tcg gat aag cct ttc ctt aat agt     144
Ser Ser Pro Tyr Val Val Tyr Pro Ser Asp Lys Pro Phe Leu Asn Ser
35                  40                  45
gat cta cga cgc tcc cca agt aag cct aca ctt gcc tat cca gaa agc     192
Asp Leu Arg Arg Ser Pro Ser Lys Pro Thr Leu Ala Tyr Pro Glu Ser
50                  55                  60
aac agc aga gcc ata ttt tct gct ctt aag aat ctt caa gat aag att     240
Asn Ser Arg Ala Ile Phe Ser Ala Leu Lys Asn Leu Gln Asp Lys Ile
65                  70                  75                  80
cga cgc ttg gaa ctt gag agg att cag gca gaa gaa agt gtg aaa acc     288
Arg Arg Leu Glu Leu Glu Arg Ile Gln Ala Glu Glu Ser Val Lys Thr
85                  90                  95
ttg tct aga gaa aca att gaa tat aag aaa gta ctg gat gaa cag ata     336
Leu Ser Arg Glu Thr Ile Glu Tyr Lys Lys Val Leu Asp Glu Gln Ile
100                 105                 110
caa gaa agg gag aat tca aag aat gag gaa tca aag cac aat caa gaa     384
Gln Glu Arg Glu Asn Ser Lys Asn Glu Glu Ser Lys His Asn Gln Glu
115                 120                 125
ctg aca tct cag ttg tta gct gca gaa aat aaa tgc aat cta tta gaa     432
Leu Thr Ser Gln Leu Leu Ala Ala Glu Asn Lys Cys Asn Leu Leu Glu
130                 135                 140
aaa caa ttg gaa tac atg cga aat atg ata aag cat gcc gaa atg gag     480
Lys Gln Leu Glu Tyr Met Arg Asn Met Ile Lys His Ala Glu Met Glu
145                 150                 155                 160
agg aca tct gtc tta gag aaa caa gtt tcc cta gaa aga gaa cga caa     528
Arg Thr Ser Val Leu Glu Lys Gln Val Ser Leu Glu Arg Glu Arg Gln
165                 170                 175
cat gat caa aca cat gtt cag agc caa ctt gaa aaa ttg gat ctt ctt     576
His Asp Gln Thr His Val Gln Ser Gln Leu Glu Lys Leu Asp Leu Leu
180                 185                 190
gaa cag gag tat aac aaa ctt acc aca atg cag gcc ctt gca gaa aaa     624
Glu Gln Glu Tyr Asn Lys Leu Thr Thr Met Gln Ala Leu Ala Glu Lys
195                 200                 205
aaa atg caa gag ttg gaa gca aaa ctc cat gaa gaa gaa cag gaa agg     672
Lys Met Gln Glu Leu Glu Ala Lys Leu His Glu Glu Glu Gln Glu Arg
210                 215                 220
aaa cgc atg caa gct aag gca gct gag ttg cag act ggt cta gaa aca     720
Lys Arg Met Gln Ala Lys Ala Ala Glu Leu Gln Thr Gly Leu Glu Thr
225                 230                 235                 240
aat aga ctt atc ttt gaa gat aag gca act ccg tgt gtt ccc aat gca     768
Asn Arg Leu Ile Phe Glu Asp Lys Ala Thr Pro Cys Val Pro Asn Ala
245                 250                 255
aga aga att aaa aaa aag aag tca aaa cca cca gaa aag aaa agt tct     816
Arg Arg Ile Lys Lys Lys Lys Ser Lys Pro Pro Glu Lys Lys Ser Ser
260                 265                 270
agg aac tat ttt ggt gca caa cca cat tat aga tta tgc ttg ggt gat     864
Arg Asn Tyr Phe Gly Ala Gln Pro His Tyr Arg Leu Cys Leu Gly Asp
275                 280                 285
atg cca ttt gta gct ggg aag tcc aca agc cct agc cat gcc gtg gta     912
Met Pro Phe Val Ala Gly Lys Ser Thr Ser Pro Ser His Ala Val Val
290                 295                 300
gcc aat gtt cag ctt gtc ttg cat cta atg aag caa cac agt aaa gct     960
Ala Asn Val Gln Leu Val Leu His Leu Met Lys Gln His Ser Lys Ala
305                 310                 315                 320
ttg tgc aat gat cga gtc atc aac agt att cct ttg gca aag caa gta    1008
Leu Cys Asn Asp Arg Val Ile Asn Ser Ile Pro Leu Ala Lys Gln Val
325                 330                 335
tct tca cga ggt ggt aaa agt aag aag ttg tca gta aca cct ccc tcc    1056
Ser Ser Arg Gly Gly Lys Ser Lys Lys Leu Ser Val Thr Pro Pro Ser
340                 345                 350
tcc aac ggt att aat gag gag ttg tca gaa gtc tta cag act tta cag    1104
Ser Asn Gly Ile Asn Glu Glu Leu Ser Glu Val Leu Gln Thr Leu Gln
355                 360                 365
gat gaa ttt ggg caa atg agc ttt gat cac cag cag ctt gca aaa ctt    1152
Asp Glu Phe Gly Gln Met Ser Phe Asp His Gln Gln Leu Ala Lys Leu
370                 375                 380
atc cag gag tcg cca acc gtt gaa ctg aaa gac aag ttg gag tgt gaa    1200
Ile Gln Glu Ser Pro Thr Val Glu Leu Lys Asp Lys Leu Glu Cys Glu
385                 390                 395                 400
ttg gag gca tta gtg gga agg atg gaa gca aaa gcc aac caa ata act    1248
Leu Glu Ala Leu Val Gly Arg Met Glu Ala Lys Ala Asn Gln Ile Thr
405                 410                 415
aaa gtt cga aaa tac caa gcc cag ctg gag aaa cag aag tta gag aag    1296
Lys Val Arg Lys Tyr Gln Ala Gln Leu Glu Lys Gln Lys Leu Glu Lys
420                 425                 430
cag aag aag gaa tta aaa gct acc aaa aag act ctt gat gaa gaa gga    1344
Gln Lys Lys Glu Leu Lys Ala Thr Lys Lys Thr Leu Asp Glu Glu Gly
435                 440                 445
aac agc agc agc cgt tct gga atc aca ggg acc aca aat aag aaa gat    1392
Asn Ser Ser Ser Arg Ser Gly Ile Thr Gly Thr Thr Asn Lys Lys Asp
450                 455                 460
ttt atg aaa ctg aga cct gga gaa aaa agg aga aaa aat ctt cag tta    1440
Phe Met Lys Leu Arg Pro Gly Glu Lys Arg Arg Lys Asn Leu Gln Leu
465                 470                 475                 480
ttg aag gac atg caa agc ata cag aat tca tta caa agc agt agt ttg    1488
Leu Lys Asp Met Gln Ser Ile Gln Asn Ser Leu Gln Ser Ser Ser Leu
485                 490                 495
tgt tgg gat tac tga                                                1503
Cys Trp Asp Tyr
500
<210> 2
<211> 500
<212> PRT
<213> Homo sapiens
<400> 2
Met Ala Ala Ala Ser Val Ser Ala Ala Ser Gly Ser His Leu Ser Asn
1               5                  10                  15
Ser Phe Ala Glu Pro Ser Arg Ser Asn Gly Ser Met Val Arg His Ser
20                  25                  30
Ser Ser Pro Tyr Val Val Tyr Pro Ser Asp Lys Pro Phe Leu Asn Ser
35                  40                  45
Asp Leu Arg Arg Ser Pro Ser Lys Pro Thr Leu Ala Tyr Pro Glu Ser
50                  55                  60
Asn Ser Arg Ala Ile Phe Ser Ala Leu Lys Asn Leu Gln Asp Lys Ile
65                  70                  75                  80
Arg Arg Leu Glu Leu Glu Arg Ile Gln Ala Glu Glu Ser Val Lys Thr
85                  90                  95
Leu Ser Arg Glu Thr Ile Glu Tyr Lys Lys Val Leu Asp Glu Gln Ile
100                 105                 110
Gln Glu Arg Glu Asn Ser Lys Asn Glu Glu Ser Lys His Asn Gln Glu
115                 120                 125
Leu Thr Ser Gln Leu Leu Ala Ala Glu Asn Lys Cys Asn Leu Leu Glu
130                 135                 140
Lys Gln Leu Glu Tyr Met Arg Asn Met Ile Lys His Ala Glu Met Glu
145                 150                 155                 160
Arg Thr Ser Val Leu Glu Lys Gln Val Ser Leu Glu Arg Glu Arg Gln
165                 170                 175
His Asp Gln Thr His Val Gln Ser Gln Leu Glu Lys Leu Asp Leu Leu
180                 185                 190
Glu Gln Glu Tyr Asn Lys Leu Thr Thr Met Gln Ala Leu Ala Glu Lys
195                 200                 205
Lys Met Gln Glu Leu Glu Ala Lys Leu His Glu Glu Glu Gln Glu Arg
210                 215                 220
Lys Arg Met Gln Ala Lys Ala Ala Glu Leu Gln Thr Gly Leu Glu Thr
225                 230                 235                 240
Asn Arg Leu Ile Phe Glu Asp Lys Ala Thr Pro Cys Val Pro Asn Ala
245                 250                 255
Arg Arg Ile Lys Lys Lys Lys Ser Lys Pro Pro Glu Lys Lys Ser Ser
260                 265                 270
Arg Asn Tyr Phe Gly Ala Gln Pro His Tyr Arg Leu Cys Leu Gly Asp
275                 280                 285
Met Pro Phe Val Ala Gly Lys Ser Thr Ser Pro Ser His Ala Val Val
290                 295                 300
Ala Asn Val Gln Leu Val Leu His Leu Met Lys Gln His Ser Lys Ala
305                 310                 315                 320
Leu Cys Asn Asp Arg Val Ile Asn Ser Ile Pro Leu Ala Lys Gln Val
325                 330                 335
Ser Ser Arg Gly Gly Lys Ser Lys Lys Leu Ser Val Thr Pro Pro Ser
340                 345                 350
Ser Asn Gly Ile Asn Glu Glu Leu Ser Glu Val Leu Gln Thr Leu Gln
355                 360                 365
Asp Glu Phe Gly Gln Met Ser Phe Asp His Gln Gln Leu Ala Lys Leu
370                 375                 380
Ile Gln Glu Ser Pro Thr Val Glu Leu Lys Asp Lys Leu Glu Cys Glu
385                 390                 395                 400
Leu Glu Ala Leu Val Gly Arg Met Glu Ala Lys Ala Asn Gln Ile Thr
405                 410                 415
Lys Val Arg Lys Tyr Gln Ala Gln Leu Glu Lys Gln Lys Leu Glu Lys
420                 425                 430
Gln Lys Lys Glu Leu Lys Ala Thr Lys Lys Thr Leu Asp Glu Glu Gly
435                 440                 445
Asn Ser Ser Ser Arg Ser Gly Ile Thr Gly Thr Thr Asn Lys Lys Asp
450                 455                 460
Phe Met Lys Leu Arg Pro Gly Glu Lys Arg Arg Lys Asn Leu Gln Leu
465                 470                 475                 480
Leu Lys Asp Met Gln Ser Ile Gln Asn Ser Leu Gln Ser Ser Ser Leu
485                 490                 495
Cys Trp Asp Tyr
500