(57)【特許請求の範囲】
【請求項１】
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(A)以下の(A1)〜(A4)から選ばれる、標的タンパク質のアミノ酸配列を決定する工程：
工程(A1)
(1)酵母を所定の培養条件下で培養する工程；
(2)該酵母の培養物からタンパク質試料を抽出する工程；
(3)該タンパク質試料をタンパク質分離手段により分離し、標的ピークまたはスポットを選択し、該ピークまたはスポットに含まれる標的タンパク質またはその断片を回収する工程；および
(4)該標的タンパク質のアミノ酸配列を決定する工程、
工程(A2)
(1)酵母を所定の培養条件下で培養する工程；
(2)該酵母の培養物からタンパク質試料を抽出する工程；および
(3)該タンパク質試料に含まれる１以上のタンパク質のアミノ酸配列を決定する工程、
工程(A3)
(1)同一の酵母の菌株を異なる培養条件下で培養する工程；
(2)工程(1)で得られる各培養物からタンパク質試料を抽出する工程；
(3)該タンパク質試料を分析して、各培養条件下で高発現または低発現のタンパク質を特定する工程；および
(4)工程(3)において特定されたタンパク質のアミノ酸配列を決定する工程、
工程(A4)
(1)異なる酵母の菌株を同一培養条件下で培養する工程；
(2)工程(1)で得られる各培養物からタンパク質試料を抽出する工程；
(3)該タンパク質試料を分析して、各培養物で発現レベルが異なるタンパク質を特定する工程；および
(4)工程(3)において特定されたタンパク質のアミノ酸配列を決定する工程、
(B)工程(A)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、
(C)該比較結果に基づいて、該標的タンパク質をコードする遺伝子を同定する工程、および
(D)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程
を包含し、該下面発酵酵母のゲノム配列情報の全部または一部が、以下：
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３
のうちの２以上のヌクレオチド配列を含む、
方法。
【請求項２】
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(1)酵母の標的タンパク質のアミノ酸配列をもとに、下面発酵酵母のゲノム配列情報の全部または一部を含むデータベースに対して参照を行う工程、
(2)該参照結果に基づいて、該タンパク質をコードする遺伝子を同定する工程、および
(3)同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程
を包含し、該下面発酵酵母のゲノム配列情報の全部または一部が、以下：
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３
のうちの２以上のヌクレオチド配列を含む、
方法。
【請求項３】
前記標的タンパク質のアミノ酸配列が、前記酵母由来のタンパク質抽出物から１以上のタンパク質を単離して該1以上のタンパク質のアミノ酸配列を決定することによって得られる、請求項２に記載の方法。
【請求項４】
前記下面発酵酵母のゲノム配列情報の全部または一部が、
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３
から選ばれる４０以上のヌクレオチド配列を含む、請求項１〜３のいずれかに記載の方法。
【請求項５】
前記下面発酵酵母のゲノム配列情報の全部または一部が、
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３
から選ばれる５０以上のヌクレオチド配列を含む、請求項１〜３のいずれかに記載の方法。
【請求項６】
前記下面発酵酵母のゲノム配列情報の全部または一部が、
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３
のヌクレオチド配列を含む、請求項１〜３のいずれかに記載の方法。
【請求項７】
前記下面発酵酵母のゲノム配列情報の全部または一部が、配列番号３３〜６２３６のヌクレオチド配列を含む、請求項１〜３のいずれかに記載の方法。
【請求項８】
前記下面発酵酵母のゲノム配列情報の全部または一部が、配列番号３３〜６２３６から選ばれる２以上のヌクレオチド配列を含む、請求項１〜３のいずれかに記載の方法。
【請求項９】
前記下面発酵酵母のゲノム配列情報の全部または一部が、配列番号１６６１５４〜１６６１８１のヌクレオチド配列を含む、請求項１〜３のいずれかに記載の方法。
【請求項１０】
前記下面発酵酵母のゲノム配列情報の全部または一部が、配列番号１６６１５４〜１６６１８１から選ばれる３以上のヌクレオチド配列を含む、請求項１〜３のいずれかに記載の方法。
【発明の詳細な説明】
【技術分野】
【０００１】
本発明は、醸造用酵母の有用タンパク質の同定方法に関する。より具体的には、本発明は、プロテオーム解析により、醸造用酵母のゲノム配列情報から醸造用酵母の有用タンパク質およびそれをコードする遺伝子を同定する方法に関する。
【０００２】
本発明はさらに、上記方法により同定された遺伝子及びその用途に関し、特に、酒類（例えば、ビール）の製造における生産性の向上および／または香味の安定化や増強等の香味の改善等に有用な醸造酵母の育種、該酵母を用いて製造した酒類、その製造方法等に関する。
【背景技術】
【０００３】
近年、遺伝子工学的な手法を用いて、目的とする遺伝子を所望の細胞に導入・発現させることが可能となっており、ゲノム情報を利用して機能解析された遺伝子を用いて、望ましい形質をもつ細胞を遺伝子工学的に作製することが行われている。
【０００４】
工業用酵母を用いた燃料用アルコール、または醸造用酵母を用いた酒類の製造の分野においても、生産性向上や、香味の安定化および／または改善を目的として、酵母を遺伝子工学的に育種することが盛んに行われている。
【０００５】
遺伝子操作のターゲットとなるタンパク質やそれをコードする遺伝子を同定するための方法のひとつとして、タンパク質を網羅的に解析するプロテオーム解析がある。例えば、生化学的特性によって分離したタンパク質をコードする遺伝子を同定し、（公知タンパク質配列に対する）データベース検索によってその機能を解明することに利用される。
【０００６】
プロテオーム解析において、現在最も良く使用されている手法の一例としては、ペプチドマスフィンガープリンティング（peptide mass-finger printing（ＰＭＦ））法が挙げられる。ＰＭＦ法では、二次元電気泳動等によってタンパク質を分離し、そのタンパク質をトリプシンなどのプロテアーゼでペプチドに消化後、得られたペプチド混合物の質量スペクトル（ペプチドマスフィンガープリント）を質量分析装置を用いて取得し、この取得した質量スペクトルとゲノムデータベースからのＤＮＡの塩基配列に対応するアミノ酸配列から計算される理論的な質量スペクトルとを比較することにより、タンパク質および該タンパク質をコードする遺伝子を同定する。
【０００７】
醸造用酵母の１つである下面発酵酵母においても、プロテオーム解析が試みられた（Joubert et al., Electrophoresis, 22, 2969 (2001)）が、下面発酵酵母のゲノムの約半分は未知であるため、網羅的な解析はできなかった。すなわち、下面発酵酵母は、少なくとも２種類のゲノムから構成されている異質倍数体である（Y. Tamai et al., Yeast, 10, 923 (1998)）。ひとつはゲノム塩基配列が解読されている（例えば、Goffeau et al., Nature, 387, 5 (1997)参照）S. cerevisiae 由来のゲノム (Saccharomyces cerevisiae型、以降Sc型と表記することもある)であるが、残りのゲノム(Non-S. cerevisiae型、以降Non-Sc型と表記することもある) は解読されていない。
【非特許文献１】Joubert et al., Electrophoresis, 22, p2969-2982, 2001
【非特許文献２】Y. Tamai et al., Yeast, 10, p923-33, 1998
【非特許文献３】A.Goffeau et al., Nature,387, p5-105, 1997
【発明の開示】
【発明が解決しようとする課題】
【０００８】
上記状況において、下面発酵酵母の網羅的なプロテオーム解析を行い、有用なタンパク質とそれをコードする遺伝子を同定して酒類の生産性の向上、および／または香味改善等に利用するために、下面発酵酵母の全ゲノムを解析することが求められている。
【課題を解決するための手段】
【０００９】
上記課題に鑑み、本発明者らは下面発酵酵母のゲノム配列情報を解読した。そし下面発酵酵母は、S. cerevisiaeゲノム塩基配列と約９４％より高い同一性を示すSc型塩基配列群と約８４％前後の同一性を示すNon-Sc型塩基配列群を有するゲノム構造を有することを明らかにした。そして既にゲノム配列が明らかにされているS. cerevisiaeのゲノムＤＢに登録されているアミノ酸配列と比較し、下面発酵酵母の遺伝子がコードするタンパク質の機能推定を行った結果、該下面発酵酵母のタンパク質は、アミノ酸配列において、S. cerevisiaeと１００％近い同一性を示すタンパク質（Sc型）と、７０〜９７％前後の同一性を示すタンパク質（Non-Sc型）に大別されることを明らかにした。そしてさらに、本発明者らは、これらの知見に基づいて検討を重ねた結果、本発明を完成するに至った。
【００１０】
すなわち、本発明は、この解読した下面発酵酵母のゲノム配列情報を基に、目的とする酵母のタンパク質またはそれをコードする遺伝子を同定する方法を提供する。
【００１１】
特に、醸造用酵母の有用なタンパク質およびそれをコードする遺伝子を同定する方法を提供する。
【００１２】
より具体的には、本発明は、以下の方法を提供する。
〔１〕酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(a)酵母を所定の培養条件下で培養する工程、
(b)該酵母の培養物からタンパク質試料を抽出する工程、
(c)該タンパク質試料をタンパク質分離手段により分離し、標的ピークまたはスポットを選択し、該ピークまたはスポットに含まれる標的タンパク質またはその断片を回収する工程、
(d)該標的タンパク質のアミノ酸配列を決定する工程、
(e)工程(d)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、
(f)該比較結果に基づいて、該標的タンパク質および該標的タンパク質をコードする遺伝子を同定する工程、および
(g)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性
を同定する工程
を包含する方法。
【００１３】
なお、本明細書中、「培養物」とは、酵母（または菌株）を培養して得られるものを広く意味し、培養液（すなわち培地）、培養沈殿物、培養液に含まれる菌体、培養上清などを含む。
【００１４】
〔２〕酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(1)酵母の標的タンパク質のアミノ酸配列をもとに、下面発酵酵母のゲノム配列情報の全部または一部を含むデータベースに対して参照を行う工程、
(2)該参照結果に基づいて、該タンパク質をコードする遺伝子を同定する工程、および
(3)同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程
を包含する方法。
〔３〕酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(1)酵母由来のタンパク質抽出物から１以上のタンパク質（酵母から直接抽出されるタンパク質、培養液中に分泌されるタンパク質などが含まれる）を分離して、該１以上のタンパク質のアミノ酸配列を決定する工程、
(2)該１以上のタンパク質のアミノ酸配列をもとに、下面発酵酵母のゲノム配列情報の全部または一部を含むデータベースに対して参照を行う工程、
(3)該参照結果に基づいて、該１以上のタンパク質をコードする遺伝子を同定する工程、および
(4)同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程
を包含する方法。
〔４〕酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(1)酵母を所定の培養条件下で培養する工程、
(2)該酵母の培養物からタンパク質試料を抽出する工程、
(3)該タンパク質試料に含まれる１以上のタンパク質のアミノ酸配列を決定する工程、
(4)工程(3)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、
(5)該比較結果に基づいて、標的タンパク質をコードする遺伝子を同定する工程、および
(6)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程
を包含する方法。
〔５〕酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(1)同一の酵母を、異なる培養条件下で培養する工程、
(2)工程(1)で得られる各培養物からタンパク質試料を抽出する工程、
(3)該タンパク質試料を分析し、各培養条件下で高発現または低発現のタンパク質を特定する工程、
(4) 工程(3)で特定されたタンパク質のアミノ酸配列を決定する工程、
(5)工程(4)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、
(6)該比較結果に基づいて、標的タンパク質をコードする遺伝子を同定する工程、および
(7)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程
を包含する方法。
〔６〕酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、
(1)異なる酵母の菌株を、同一培養条件下で培養する工程、
(2)工程(1)で得られる各培養物からタンパク質試料を抽出する工程、
(3)該タンパク質試料を分析し、各培養物で発現量が異なる（高発現または低発現）タンパク質を特定する工程、
(4)工程(3)で特定されたタンパク質のアミノ酸配列を決定する工程、
(5)工程(4)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、
(6)該比較結果に基づいて、該標的タンパク質をコードする遺伝子を同定する工程、および
(7)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程
を包含する方法。
〔７〕前記下面発酵酵母のゲノム配列情報の全部または一部が、
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３の塩基配列を含む、上記〔１〕〜〔６〕のいずれかに記載の方法。
〔８〕前記下面発酵酵母のゲノム配列情報の全部または一部が、
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３から選ばれる２以上の塩基配列を含む、上記〔１〕〜〔６〕のいずれかに記載の方法。
〔９〕前記下面発酵酵母のゲノム配列情報の全部または一部が、配列番号３３〜６２３６の塩基配列を含む、上記〔１〕〜〔６〕のいずれかに記載の方法。
〔１０〕前記下面発酵酵母のゲノム配列情報の全部または一部が、  配列番号３３〜６２３６から選ばれる２以上の塩基配列を含む、上記〔１〕〜〔６〕のいずれかに記載の方法。
〔１１〕前記下面発酵酵母のゲノム配列情報の全部または一部が、  配列番号１６６１５４〜１６６１８１の塩基配列を含む、上記〔１〕〜〔６〕のいずれかに記載の方法。
〔１２〕前記下面発酵酵母のゲノム配列情報の全部または一部が、配列番号１６６１５４〜１６６１８１から選ばれる２以上の塩基配列を含む、上記〔１〕〜〔６〕のいずれかに記載の方法。
【００１５】
本発明の方法により、所望の醸造特性に関わる遺伝子およびそれによりコードされるタンパク質を効率よく同定することができる。これにより、下面発酵酵母のゲノムに含まれる遺伝子の翻訳産物の網羅的な機能解析が可能になる。
【００１６】
さらに、本発明に従って、醸造用酵母の醸造特性に関わると考えられる遺伝子の塩基配列を同定し、その遺伝子を遺伝子工学的に破壊、および／または高発現させることによって、酵母の醸造特性を制御することができる。
【００１７】
このようにして、本発明によれば、有用な醸造特性を発揮する酵母を育種し、この酵母を用いて生産性や品質を向上させた燃料用アルコール又は酒類の製造が可能となる。例えば、本発明に従って同定されたNon-ScMET17遺伝子を用いて、該遺伝子を高発現するように改変され、硫化水素生成量が減少された酵母を作製することができる。このような酵母を使用することにより製品中の硫化水素濃度が低く抑えられ、香味の優れた酒類を製造することが可能となる。
【発明を実施するための最良の形態】
【００１８】
１．本発明の概要
本発明者らは、特開2004-283169にその概要が示された方法で解析したゲノム配列を基に、標的とする酵母タンパク質およびそれをコードする遺伝子を同定するためのデータベースを作成した。なお、このデータベースは、本件出願の優先日の時点で非公知である。
【００１９】
このデータベースには、下面発酵酵母のゲノム塩基配列情報が含まれる。このデータベースは、具体的には、添付の配列表に記載の塩基配列情報を含むものである。添付の配列表に記載の塩基配列情報（下面発酵酵母ゲノムの塩基配列情報）の内容は、以下の通りである。
【００２０】
配列番号３３〜６２３６は、６２０４個のＮｏｎ-Ｓｃ型オープンリーディングフレーム（以下ＯＲＦと表記することもある）を表す。配列番号７５３３７〜８２７８４は、７４４８個のＳｃ型ＯＲＦを表す。配列番号１６６１５４〜１６６１８１は、下面発酵酵母の２８個のミトコンドリアＯＲＦの塩基配列を表す。配列番号１６６４９０〜１６７０４２は、上記ＯＲＦとして同定されていない配列であるが、NCBI-BlastX(http://www.ncbi.nlm.nih.gov/BLAST/)の相同性検索を用いてS.cerevisiaeの遺伝子に対して有意な類似性を有する５５３個の塩基配列を表す。配列番号１７３１２５〜１７４６０３は、上記以外のＯＲＦとして同定されており、その翻訳産物がNCBI-BlastP(http://www.ncbi.nlm.nih.gov/BLAST/)の相同性検索を用いてS.cerevisiaeのタンパク質に対して有意な類似性を有する１４７９個の塩基配列を表す。
【００２１】
このような酵母のゲノム配列情報に基づくデータベースを使用すれば、酵母の標的タンパク質（または機能未知タンパク質）またはそれをコードする遺伝子をプロテオーム解析の手法によって特定することができる。プロテオーム解析の手法としては、例えば、タンパク質の分離精製手段（例、二次元電気泳動（２−ＤＥ）、高性能液体クロマトグラフィ（ＨＰＬＣ）など）により標的タンパク質を分離精製し、得られた標的タンパク質をタンパク質同定手段（例、質量分析により得られたペプチドマップによる比較、アミノ酸配列分析により得られたアミノ酸配列の比較など）によって特定する方法などがあげられる。標的タンパク質の選択は、公知の方法〔例えば、二次元泳動（２−ＤＥ）または蛍光二次元ディファレンシャル電気泳動（２Ｄ−ＤＩＧＥ）、同位体標識法(ＩＣＡＴ法)など〕を利用して行うことができる。なお、ショットガン分析法を用いることによって、タンパク質試料中の機能未知タンパク質の機能を網羅的に解析することも可能となる。
【００２２】
したがって、本発明は、新規な下面発酵酵母のゲノム配列情報を用いて、プロテオーム解析の手法に基づいて酵母の標的タンパク質（または機能未知タンパク質）およびそれをコードする遺伝子を特定する方法を提供する。さらに、必要があれば、その特定された標的タンパク質およびそれをコードする遺伝子の機能を、公知の方法により解析することもできる。
【００２３】
より具体的には、本発明は、次のような態様の方法を包含する。
（態様１）
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、(a)酵母を所定の培養条件下で培養する工程、(b)該酵母の培養物からタンパク質試料を抽出する工程、(c)該タンパク質試料をタンパク質分離手段により分離し、標的ピークまたはスポットを選択し、該ピークまたはスポットに含まれる標的タンパク質またはその断片を回収する工程、(d)該標的タンパク質のアミノ酸配列を決定する工程、(e)工程(d)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、(f)該比較結果に基づいて、該標的タンパク質をコードする遺伝子を同定する工程、および(g)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程、を包含する方法。
（態様２）
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、(1)酵母の標的タンパク質のアミノ酸配列〔標的タンパク質のアミノ酸配列を特定し得る情報、例えば、質量分析装置を用いて得られるタンパク質の質量スペクトルパターンを含む〕をもとに、下面発酵酵母のゲノム配列情報の全部または一部を含むデータベースに対して参照を行う工程、(2)該参照結果に基づいて、該タンパク質をコードする遺伝子を同定する工程、及び(3)同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程を包含する方法。
（態様３）
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、(1)酵母由来のタンパク質抽出物（酵母から直接抽出されたタンパク質、培養液中に分泌されたタンパク質等が含まれる）から１以上のタンパク質を分離して、該１以上のタンパク質のアミノ酸配列を決定する工程（アミノ酸配列特定情報を取得する工程を含む）、(2)該１以上のタンパク質のアミノ酸配列（アミノ酸配列特定情報を含む）をもとに、下面発酵酵母のゲノム配列情報の全部または一部を含むデータベースに対して参照を行う工程、(3)該参照結果に基づいて、該１以上のタンパク質をコードする遺伝子を同定する工程、および(4)同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程を包含する方法。
（態様４）
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、(1)酵母を所定の培養条件下で培養する工程、(2)該酵母の培養物からタンパク質試料を抽出する工程、(3)該タンパク質試料に含まれる１以上のタンパク質のアミノ酸配列を決定する工程、(4)工程(3)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、(5)該比較結果に基づいて、標的タンパク質をコードする遺伝子を同定する工程、および(6)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程、を包含する方法。
（態様５）
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、(1)同一の酵母株を、異なる培養条件下で培養する工程、(2)工程(1)で得られる各培養物からタンパク質試料を抽出する工程、(3)該タンパク質試料を分析し、各培養条件下で高発現または低発現のタンパク質を特定する工程、(4) 工程(3)で特定されたタンパク質のアミノ酸配列を決定する工程、(5)工程(4)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、(6)該比較結果に基づいて、標的のタンパク質をコードする遺伝子を同定する工程、および(7)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程、を包含する方法。
（態様６）
酵母の標的タンパク質またはそれをコードする遺伝子を同定する方法であって、(1)異なる酵母株を、同一培養条件下で培養する工程、(2)工程(1)で得られる各培養物からタンパク質試料を抽出する工程、(3)該タンパク質試料を分析し、各培養物で発現量が異なる（高発現または低発現）タンパク質を特定する工程、(4)工程(3)で特定されたタンパク質のアミノ酸配列を決定する工程、(5)工程(4)において決定されたアミノ酸配列と、下面発酵酵母のゲノム配列情報の全部または一部に基づいて、予め決定しておいたアミノ酸配列とを比較する工程、(6)該比較結果に基づいて、該標的タンパク質をコードする遺伝子を同定する工程、および(7)該同定された遺伝子の機能解析を行うことによって該遺伝子が酵母に付与する特性を同定する工程、を包含する方法。
【００２４】
本明細書中、「標的タンパク質」とは、本発明によって特定しようとする有用タンパク質であって、その全部または一部のアミノ酸配列が、参照配列データベースを用いた検索の対象となるタンパク質のことをいう。標的タンパク質は、機能未知のタンパク質を含む。代表的な標的タンパク質としては、酵母のタンパク質が挙げられる。目的とする酵母のタンパク質の代表的な例としては、例えば、醸造用酵母（例：S.pastorianusに属する下面発酵酵母、S.cerevisiaeに属する上面発酵酵母等）、パン酵母のタンパク質等が挙げられるが、これらに限定されない。好ましい目的タンパク質としては、例えば、ビールの醸造に有用なタンパク質が挙げられる。
【００２５】
本明細書中、「醸造用酵母」とは、酒類の醸造用に使用可能な任意の酵母のことをいい、例えばビール、ワイン、清酒、ウイスキー、焼酎等の醸造用の酵母等が挙げられる。
【００２６】
なお、ビールの種類は、酵母の種類と発酵の方法によって大きく３つに分けられ、醸造所に棲み付いた酵母や微生物を利用して発酵させる自然発酵のビール、S.cerevisiaeに属する上面発酵酵母を用いて２０〜２５℃の温度で発酵し、その後の熟成期間を短くするエールタイプのビール、およびサッカロマイセス  パストリアヌス（Saccharomyces pastorianus）に属する下面発酵酵母を用いて６〜１５℃の温度で発酵させ、その後低温熟成させるラガータイプのビールがある。ラガータイプのビールの醸造に使用されている下面発酵酵母が、ビール醸造において最も広く用いられている。
【００２７】
本明細書中、「タンパク質の一部」とは、そのタンパク質の断片（例えば、タンパク質のプロテアーゼ消化により生じるペプチド断片）を意味する。このようなタンパク質断片の長さは、その断片のアミノ酸配列を利用した参照データベース解析によって、元のタンパク質を同定できるように少なくとも４アミノ酸残基からなることが好ましい（例えば、Wilkins et al., J.Mol.Biol., 278, 599(1998)を参照）。なお、タンパク質の断片には、ペプチド合成機による合成ポリペプチドも含まれ得る。
【００２８】
本明細書中、「アミノ酸配列を決定する」という表現は、標的タンパク質の全部または一部のアミノ酸配列を決定することのみならず、標的タンパク質のアミノ酸配列を特定し得る情報（例えば、質量分析装置を用いて得られるタンパク質の質量スペクトルパターン）を取得することを含む意味で用いられる。標的タンパク質の全アミノ酸配列を決定することが好ましいが、参照データベース中のアミノ酸配列と比較し、類似配列を抽出できる程度の長さのアミノ酸配列が決定することができれば本発明を実施することができる。たとえば、概標的タンパク質とヒットした参照データベース中のアミノ酸配列において、同定ソフトウェア、例えばMASCOTで期待値(P-value)が0.05未満のフラグメントが少なくとも１つ以上存在し、標的タンパク質の全長の ５％、１０％、２０％、３０％、４０ ％、５０％以上、６０％以上、７０％以上、８０％以上、９０％以上、９５％以上、９７％以上をカバーする場合は標的タンパク質がスクリーニングされたとみなすことが出来る（大きい値ほど好ましい）。また、「タンパク質の全部または一部のアミノ酸配列」は、検索対象のタンパク質の全部または一部のアミノ酸配列のことをいい、これには、例えば、既にアミノ酸配列が既知のタンパク質またはその断片のアミノ酸配列、アミノ酸配列が未知のタンパク質またはその断片で、そのアミノ酸配列を、例えばプロテオーム解析手法に従った質量分析もしくはその他の方法により明らかにした該タンパク質またはその断片のアミノ酸配列などが含まれる。また、質量分析装置を用いて得られるそのタンパク質の質量スペクトルパターン（本明細書において「質量パターン」と略記することもある。）からアミノ酸配列が理論的に導かれるが、そのような質量パターンおよびそのような質量パターンから理論的に推定されるアミノ酸配列も、本明細書中、上記タンパク質の全部または一部のアミノ酸配列と同等のものと見なす。したがって、「タンパク質のアミノ酸配列を決定する」という場合、このような質量パターンからタンパク質の全部または一部のアミノ酸配列を推定することも含まれるものとする。質量パターンは、通常、分析に供したポリペプチド試料中の各ペプチドのピークが、横軸にイオン化したペプチドまたはタンパク質のｍ／ｚ（質量／電荷）、縦軸に相対強度をとって表される。質量パターンからのアミノ酸配列の理論的導出は、通常専用のソフトウェア（例えば、ＭＡＳＣＯＴ）を用いて行われる。
【００２９】
本明細書中、「参照データベース」または「参照配列データベース」とは、下面発酵酵母のゲノム配列情報の全部または一部を含む塩基配列データベース、またはこの塩基配列データベースから理論的に翻訳されたアミノ酸配列データベースのことをいう。「理論的に翻訳されたアミノ酸配列」とは、核(染色体)ＤＮＡ上の遺伝子にコードされているアミノ酸の場合、普遍的遺伝暗号（universal genetic code）に、ミトコンドリアゲノム上の遺伝子にコードされているアミノ酸の場合、The Yeast Mitochondrial Code（http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi?mode=c#SG3）に従って所定のヌクレオチド配列によってコードされることが予想されるアミノ酸配列のことをいうものとする。
【００３０】
本明細書中、「下面発酵酵母のゲノム配列情報の全部」とは、下面発酵酵母のゲノムの塩基配列全体を指す。また、「下面発酵酵母のゲノム配列情報の一部」とは、下面発酵酵母のゲノムの塩基配列の少なくとも一部（例えば、少なくとも１つのコンティグ塩基配列）を指す。「下面発酵酵母のゲノムの塩基配列の少なくとも一部」の長さは、８塩基以上であり、より好ましくは、１２塩基以上、１５塩基以上、１８塩基以上、２１塩基以上、２４塩基以上、２７塩基以上、または３０塩基以上であり、さらにより好ましくは、４０塩基以上、５０塩基以上、６０塩基以上、７０塩基以上、８０塩基以上、９０塩基以上、または１００塩基以上であり、なおさらにより好ましくは、２００塩基以上、３００塩基以上、４００塩基以上、５００塩基以上、６００塩基以上、７００塩基以上、８００塩基以上、または９００塩基以上であり、最も好ましくは、１０００塩基以上である。下面発酵酵母のゲノム配列情報の一部を含む塩基配列データベースの例としては、下面発酵酵母ゲノムの少なくとも１つのコンティグの塩基配列を含む塩基配列データベース、下面発酵酵母ゲノムの少なくとも１つのオープンリーディングフレーム塩基配列を含む塩基配列データベース、推定される機能別に分類した少なくとも１つのオープンリーディングフレームの塩基配列を含む塩基配列データベースなどが挙げられる。ゲノムの塩基配列のオープンリーディングフレームは、例えば、ORF Finderのようなプログラム（http://www.ncbi.nih.gov/gorf/gorf.html）を使用して同定することができる。
本明細書中、「遺伝子の機能」とは遺伝子の機能および／または遺伝子の翻訳産物の機能を含む意味で用いられる。
【００３１】
２．標的タンパク質の選択
本発明は酵母の標的タンパク質またはそれをコードする遺伝子を同定すること、および／または、その機能を同定することを目的とするものであって、その標的タンパク質の選択方法は特に限定されない。本発明においては、通常、酵母由来のタンパク質抽出物、例えば酵母の培養物または酵母菌体自体から標的タンパク質を取得する。しかし、本発明は、タンパク質のアミノ酸配列を手がかりに、酵母の標的タンパク質または、それをコードする遺伝子またはその機能をプロテオーム解析の手法によって解析することができる。ゆえに、標的タンパク質の取得方法が不明であったとしても、そのタンパク質のアミノ酸配列がわかれば、そのタンパク質またはそれをコードする遺伝子を同定し、および／または、その機能を解析することができる。
【００３２】
より具体的には、本発明においては、酵母を所定の培養条件下で培養して得られる培養物からタンパク質試料を調製する。
【００３３】
本発明においては、適用するプロテオーム解析手法に応じて、２種以上の培養物から得られるタンパク質試料を調製しておく場合もある。例えば、同一の酵母を、異なる培養条件下で培養して、それぞれの培養物からタンパク質試料を調製する場合もあるし、異なる酵母を、同一培養条件下で培養して、それぞれの培養物からタンパク質試料を調製する場合もある。
【００３４】
本明細書中、「所定の培養条件」とは、酵母細胞を培養できる条件であればよく、その組成等は限定されない。したがって、「所定の培養条件」としては、液体培地もしくは固体培地に酵母細胞を培養するのに好適な条件、または培養物に対する特定の効果を調べたい培養条件を適宜選択すればよい。
【００３５】
培養条件としては、培養温度、培養時間、培養雰囲気、ｐＨ、塩類、炭素源、窒素源、ビタミン等の酵母細胞の生存や増殖を維持するために必要な物質の濃度（又は培地の種類）、などが挙げられる。なお、種々の市販の液体培地を使用して良いことはもちろんである。例えば、ＹＰＤ培地（２％（ｗ／ｗ）グルコース、１％（ｗ／ｗ）酵母エキス、２％（ｗ／ｗ）ポリペプトン）等を用いることができる。培養方法としては、通常、約２５〜３５℃で終夜振盪培養することなどが好ましい。特殊培養条件下でその機能を発揮するタンパク質またはそれをコードする遺伝子をターゲットとする場合は、その機能に応じて培養条件を変化させる。例えば、低温耐性に関与するタンパク質またはそれをコードする遺伝子をターゲットとする場合は、酵母を低温で培養し、その培養条件下で高発現するタンパク質を標的タンパク質とすることができる。
【００３６】
３．タンパク質試料からのタンパク質の抽出
本発明の方法では、上記のようにして得られる培養物または酵母菌体からタンパク質試料が抽出される。抽出方法としては、当業者に周知の一般的なタンパク質抽出方法が使用し得る。例えば、O'Farrell（J.Biol.Chem.,250,4007-4021(1975)）の方法が用いられ得、例えば、８Ｍ  尿素、２％  ＮＰ−４０、２％  両性担体、および５％  ２−メルカプトエタノールを含む溶解液中で、試料を溶解し、タンパク質が抽出される。なお、通常、菌体からのタンパク質の抽出を促進するために、抽出前に、培養した酵母菌体を破砕バッファー（例えば、10mmol/l  Tris-HCl  pH7.4、5mmol/l  塩化マグネシウム、50mg/l  RNaseA、1.6mg/ml  プロテアーゼ・インヒビター（COMPLETE: Boehringer Mannheim Co.））中に懸濁して破砕装置を用いて菌体を破砕し、遠心して得られる上澄み液を上記タンパク質の抽出に用いる。
【００３７】
タンパク質抽出過程での酵母菌体に含まれるプロテアーゼによるタンパク質の分解を抑制するために、抽出は低温（例えば、４℃）で行い、また、プロテアーゼ阻害剤を溶解液に添加することが好ましい。このようなプロテアーゼ阻害剤としては、ＰＭＳＦ、キモスタチンのようなセリンプロテアーゼ阻害剤、ロイペプチンのようなシステインプロテアーゼ阻害剤、ペプスタチンＡのようなアスパラギン酸プロテアーゼ阻害剤、フォスフォラミドン、ＥＤＴＡ・Ｎａ2のようなメタロプロテアーゼ阻害剤等が、単独または組み合わせて用いられ得る。なお、市販の種々のプロテアーゼ・インヒビター（例えば、COMPLETE: Boehringer Mannheim Co.）を使用しても良い。
【００３８】
４．タンパク質の分離
本発明の方法では、次いで、抽出したタンパク質は、タンパク質分離手段により分離される。本明細書中、「タンパク質分離手段」とは、タンパク質試料中に含まれる種々のタンパク質を、各タンパク質の分子量および／または電荷などによって分離する手段をいう。例えば、電気泳動（例：ＳＤＳ−ＰＡＧＥ、等電点電気泳動、二次元電気泳動）、液体クロマトグラフィー（例：イオン交換クロマトグラフィー、ゲルろ過クロマトグラフィー、アフィニティークロマトグラフィー、ＨＰＬＣ、ＦＰＬＣ）、ガスクロマトグラフィー、遠心分離（例：超遠心分離）、沈殿（例：硫安分画、有機溶媒沈殿（例：アセトン沈殿、エタノール沈殿））、ｐＨ処理（例：酸処理）、膜分画（例：限外ろ過）などが、「タンパク質分離手段」に含まれる。
【００３９】
本発明においては、例えば、二次元電気泳動、蛍光二次元ディファレンシャル電気泳動などを用いて好ましく標的タンパク質を分離することができる。
【００４０】
簡便で好ましいタンパク質分離手段としては、二次元電気泳動が挙げられる。二次元電気泳動とは、ディスクあるいは平板ゲルを用いて一次元目のゲル電気泳動を行う。その後一次元目とは原理あるいは条件の異なる電気泳動の平板ゲル上部に一次元目のゲルを設置し、一次元目の方向と垂直の方向に電気泳動を行ってタンパク質を分離しようとする方法である。本発明においては、例えば、O'Farrell（1975（前出））による二次元電気泳動（通常、一次元目に等電点電気泳動を行い、二次元目にＳＤＳ−ＰＡＧＥを行う。）が好適に用いられる。また、二次元電気泳動のほかに、液体クロマトグラフィー（ＬＣ）も、本発明において好適に用いられ得る。
【００４１】
ゲル電気泳動で分離したタンパク質は、クーマシーブルー染色あるいは銀染色などにより染色して検出される。他の色素（例えば、アミドブラック、ポンソーＳ、蛍光色素など）でタンパク質を染色することもできる。種々の染色用キットが市販されている。
【００４２】
次いで、本発明の方法では、タンパク質分離手段により分離されたタンパク質について、その分離パターン（または分離プロファイル）を解析し、標的ピークまたはスポットを選択し、該ピークまたはスポットに含まれる標的タンパク質またはその断片を回収する。ここで、「分離パターン（または分離プロファイル）」とは、タンパク質分離手段により分離されたタンパク質の示す溶出プロファイルや二次元電気泳動パターンなどのことを意味する。例えば、タンパク質試料を液体クロマトグラフィーにより分離した場合には、溶出されるタンパク質の溶出プロファイル、二次元電気泳動によりタンパク質試料を分離した場合には、ゲル上に分布した種々のタンパク質のスポットのパターン（またはプロファイル）のことを意味する。通常、分離された各タンパク質の分子量は、分離パターンにおけるピークまたはスポットの位置（もしくは移動度）によって示され、また各タンパク質の濃度は、各ピークまたはスポットの強度によって示される。これらのピークまたはスポットの中から、そのピークまたはスポットの位置、移動度、強度などに基づいて、目的のピークまたはスポット（標的ピークまたは標的スポット）を選択する。
【００４３】
上記培養工程では、必要に応じて、所望の特性を有する酵母細胞を選択可能なように、酵母細胞の培養に適した通常の培養条件（非ストレス条件）と比較して１つ以上のパラメーター（例えば、温度、浸透圧、エタノール添加等）が高くまたは低く設定された条件（ストレス条件）下においてそれぞれ独立に酵母細胞の培養を行っても良い。ストレス条件下で培養した酵母株由来のタンパク質試料の分離パターンは、非ストレス条件下で培養した酵母株由来のタンパク質試料の分離パターンと比較され、その結果、非ストレス条件下よりも、ストレス条件下において高発現または低発現するタンパク質のピークまたはスポットが選択される。
【００４４】
さらに別の実施形態では、醸造用酵母の野生型株と、変異株または野生型株と比較して所望の特性を顕著に奏する株とを、酵母の培養に適した条件下で培養してもよい。これらの培養物から抽出したタンパク質試料をタンパク質分離手段により分離し、野生型株由来のタンパク質試料の分離パターンと、変異株または野生型株と比較して所望の特性を顕著に奏する株由来のタンパク質試料の分離パターンとを比較し、そして野生型株においてよりも、変異株または所望の特性を野生型株と比較して顕著に奏する株において高発現（または低発現）するタンパク質のピークまたはスポットが選択される。所望の特性を野生型株と比較して顕著に奏する株としては、例えば、ビール香味成分を野生型株に比較してより多く生成する株等が挙げられる。このようにして、変異株または所望の特性を野生型株と比較して顕著に奏する株の発現型の原因となるタンパク質またはそれをコードする遺伝子を同定することに用いることができる。
【００４５】
さらに別の実施形態では、醸造用酵母の培養物から抽出したタンパク質試料をタンパク質分離手段により分離し、標的ピークまたはスポットを選択し、タンパク質試料の分離パターンを、ゲノム配列情報が既知であるS.cerevisiaeの酵母株から抽出されたタンパク質試料の分離パターンと比較する。次いで、該ピークまたはスポットに含まれる標的タンパク質またはその断片を回収し、醸造用酵母の分離パターンに特徴的なタンパク質のピークまたはスポットが選択される。これらのスポットのタンパク質のアミノ酸配列を決定し、該アミノ酸配列に対応する塩基配列を下面発酵酵母のゲノム配列のオープンリーディングフレームのデータベースにおいて検索・同定することによって、下面発酵酵母に特有のタンパク質またはそれをコードする遺伝子を効率的に同定することができる。なお、S.cerevisiaeの酵母株から抽出されたタンパク質試料の分離パターンは、予め二次元電気泳動データベースとしてデータベース化されたものを使用してもよい。
【００４６】
さらに別の実施形態では、有用なビール酵母タンパク質を同定する目的で、ビール醸造液中からサンプリングした酵母菌体からタンパク質を抽出し、目的のタンパク質を選択することもできる。具体的には、下面発酵酵母株を用いたビール発酵を行い、発酵開始時点から経時的に発酵液をサンプリングし、酵母増殖量、外観エキス濃度等を観察する。これと並行して、サンプリングした発酵液から酵母菌体を遠心分離により回収し、回収した酵母菌体を用いてタンパク質を抽出する。さらに発酵（培養）上清も回収し、回収した発酵上清を用いてアミノ酸組成の分析を行う。菌体から抽出したタンパク質は、さらに、タンパク質分離手段により分離し、分離パターンまたは分離プロファイルを作成する。発酵開始から経時的にサンプリングした菌体のうち２以上の異なる時点で（例えば、８時間後および３２時間後に）サンプリングした菌体由来のタンパク質抽出物の分離パターンまたは分離プロファイルを比較し、例えば、発酵上清のアミノ酸分析から得た、例えば、バリン、ロイシン、イソロイシン、メチオニン等の含量の減少に伴って強度が増すスポットまたはピークを選択することができる。なお、ビールに限らず、ワイン、清酒等の醸造液または発酵液中からも同様に目的のタンパク質を選択することができる。
【００４７】
次いで、標的ピークまたはスポットに含まれる標的タンパク質またはその断片が回収される。二次元電気泳動によって分離されたタンパク質をペプチドマスフィンガープリンティングを行ったり、質量分析によりアミノ酸配列分析を行ったりする場合、通常、分離されたタンパク質をゲル中で、または二次元電気泳動で分離後、膜フィルターに転写したタンパク質を膜フィルター上で、プロテアーゼまたは化学的分解法を用いて特異的に断片化する。ゲル内消化法も、膜フィルター上での消化法もいずれも本発明において使用され得る。
【００４８】
ゲル内消化法としては、Eckerskorn-Lottspeichの方法（Eckerskorn, C. & Lottspeich, F., Chromatographia, 28, 92(1989)）またはその改良法が使用され得る。Eckerskorn-Lottspeichの方法では、クーマシーブルー等で染色したゲル上の目的のスポット（標的タンパク質部分）を含むゲル画分を切り取り、アルカリ液に浸す。次いで、ゲル画分を乾燥させ、乾燥ゲルにプロテアーゼ溶液を加えてゲルを再膨潤させることによりプロテアーゼをゲル中に浸透させ、プロテアーゼとタンパク質を接触させて消化する。プロテアーゼとしては、トリプシン、リシルエンドペプチダーゼ等が使用され得る。膜フィルター上でのタンパク質の断片化は、二次元電気泳動で分離されたタンパク質をブロットする際、ゲルとブロッティング膜フィルターの間にトリプシンやリシルエンドペプチダーゼを結合させたＰＶＤＦ膜を挟むことによって行うことができる。このようにすれば、タンパク質がゲルからブロッティング膜フィルターに転写される間に分解させることができ、タンパク質は、ペプチドとしてブロッティング膜フィルターにブロットされる。
【００４９】
なお、本発明においては、蛍光二次元ディファレンシャル電気泳動（２Ｄ−ＤＩＧＥ）、同位体標識法(ＩＣＡＴ法；Nat Biotechnol 17,994 (1999)を用いて標的タンパク質を分離することもできる。２Ｄ−ＤＩＧＥまたはＩＣＡＴ法によれば、異なる２種類の培養物から得られるタンパク質それぞれに、異なる蛍光標識または同位体標識を付すことができるので、両培養物において異なる発現をしているタンパク質を容易に見出すことができるという利点がある。
【００５０】
５．標的タンパク質のアミノ酸配列の決定
本発明の方法では、次いで、標的タンパク質またはその断片のアミノ酸配列を決定する。アミノ酸配列の決定は、通常、質量分析装置（ＭＳ）を用いて行われる。質量分析法は、ＭＳを用いてタンパク質やペプチドのような試料をイオン化し、得られた質量／電荷（ｍ／ｚ）に従って分離し、その強度を測定することにより、試料の質量を決定する方法である。イオン化には、マトリクスアシステッドレーザーデソープションイオン化法（ＭＡＬＤＩ）、エレクトロスプレーイオン化法（ＥＳＩ）、気相法（ＥＩ，ＣＩ）、電界脱離（ＦＤ）法など種々の方法が使用され得る。イオン分離には、イオン化法と相性のよいイオン分離法が用いられ、例えば、ＭＡＬＤＩの場合には、飛行時間型（time-of-flight：ＴＯＦ）質量分析計、ＥＳＩの場合には、四重極型（ＱＭＳ）、イオントラップ型、磁場型などの質量分析計がそれぞれ用いられる。質量分析装置は、タンデムで用いられることもある。例えば、ＬＣ−ＥＳＩ  ＭＳ／ＭＳ、Ｑ−ＴＯＦ  ＭＳ、ＭＡＬＤＩ−ＴＯＦ  ＭＳ等が挙げられる。なお、その他のアミノ酸配列決定法、例えば、シークエンサー（例：気相シークエンサー）によるアミノ酸配列決定法が利用されてもよい。
【００５１】
ＭＳにおいて、上記のようにプロテアーゼにより分解して得られたペプチド混合物の質量スペクトル（ペプチドマスフィンガープリント）を作製する。使用される質量分析法としては特に限定されないが、代表的な例を挙げれば、ＭＡＬＤＩ−ＴＯＦ  ＭＳ、ＥＳＩ  Ｑ−ＴＯＦ  ＭＳなどがある。得られたペプチドマスフィンガープリントと、タンパク質データベースのアミノ酸配列から計算される理論的なスペクトルとを比較することにより、二次元電気泳動により分離されたタンパク質がどのようなタンパク質であるかを明らかにする（例：アミノ酸配列決定）ことができる。さらに、ＤＮＡデータベースの塩基配列に対応するアミノ酸配列から計算される理論的な質量スペクトルと比較することにより、そのタンパク質をコードする遺伝子を同定することができる。このような処理は、通常、専用のソフトウェア（例：MASCOT）によって行われ得る。
【００５２】
なお、本発明のプロテオーム解析においては、ショットガン分析法（Nat. Biotechnol. 17,676 (1999)）を用いることもできる。ショットガン分析法では、抽出したタンパク質をプロテアーゼで消化し、消化物を多次元ＬＣで分離し、ＭＳ／ＭＳで消化物中のペプチドのアミノ酸配列を分析し、大量のシークエンスデータをコンピュータで分析し、順次タンパク質を同定していく。このショットガン分析法を利用すれば、ある酵母の培養物から機能未知タンパク質をまとめて解析できるという利点がある。
【００５３】
６．遺伝子の同定
上記のようにして標的タンパク質（又はその一部）のアミノ酸配列を決定したら、そのアミノ酸配列（又は部分配列）から、それをコードする遺伝子を、参照データベースを参照することによって決定する。
【００５４】
本発明において用いる参照データベースには、下面発酵酵母のゲノム塩基配列情報の全部または一部が含まれる。
【００５５】
本発明において使用する参照データベースの例を以下に記載する。
（データベース・参考例１）
標的タンパク質または概標的タンパク質をコードする遺伝子の同定、更に、遺伝子の翻訳産物の機能解析を網羅的に行う目的には、代表的には、以下の配列番号の塩基配列を含む下面発酵酵母のゲノム配列データベースが挙げられる：
配列番号３３〜６２３６、
配列番号７５３３７〜８２７８４、
配列番号１６６１５４〜１６６１８１、
配列番号１６６４９０〜１６７０４２、および
配列番号１７３１２５〜１７４６０３。
【００５６】
（データベース・参考例２）
また、データベースは、上記の各グループごとに分けて構成することも可能であり、例えば、Non-Sc型の下面発酵酵母遺伝子の翻訳産物の機能解析を行う目的には、以下の配列番号の配列またはそれらのうちの１つまたは複数の配列を含むデータベースが使用され得る：
配列番号３３〜６２３６。
【００５７】
（データベース・参考例３）
さらに、例えば、Ｓｃ型の下面発酵酵母遺伝子の翻訳産物の機能解析を行う目的には、以下の配列番号の配列またはそれらのうちの１つまたは複数の配列を少なくとも含むデータベースが使用され得る：
配列番号７５３３７〜８２７８４。
【００５８】
（データベース・参考例４）
さらに、例えば、ミトコンドリアＯＲＦの翻訳産物の機能解析を行う目的には、以下の配列番号の配列またはそれらのうちの１つまたは複数の配列を少なくとも含むデータベースが使用され得る：
配列番号１６６１５４〜１６６１８１。
【００５９】
（データベース・参考例５）
本発明の目的に使用するためのデータベースの例としては、上記のものに限定されず、例えば、配列番号３３〜６２３６、配列番号７５３３７〜８２７８４、配列番号１６６１５４〜１６６１８１、配列番号１６６４９０〜１６７０４２、および配列番号１７３１２５〜１７４６０３の塩基配列の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２５以上、３０以上、３５以上、４０以上、４５以上、５０以上、５５以上、６０以上、６５以上、７０以上、７５以上、８０以上、８５以上、９０以上、９５以上、１００以上、２００以上、３００以上、４００以上、５００以上、６００以上、７００以上、８００以上、９００以上、１０００以上、１１００以上、１２００以上、１３００以上、１４００以上、１６００以上、１７００以上、１８００以上、１９００以上、２０００以上、２１００以上、２２００以上、２３００以上、２４００以上、２５００以上、２６００以上、２７００以上、２８００以上、２９００以上、３０００以上、３１００以上、３２００以上、３３００以上、３４００以上、３５００以上、３６００以上、３７００以上、３８００以上、３９００以上、４０００以上、４１００以上、４２００以上、４３００以上、４４００以上、４５００以上、４６００以上、４７００以上、４８００以上、４９００以上、５０００以上、５１００以上、５２００以上、５３００以上、５４００以上、５５００以上、５６００以上、５７００以上、５８００以上、５９００以上、６０００以上、６１００以上、６２００以上、６３００以上、６４００以上、６５００以上、６６００以上、６７００以上、６８００以上、６９００以上、７０００以上、７１００以上、７２００以上、７３００以上、７４００以上、７５００以上、７６００以上、７７００以上、７８００以上、７９００以上、８０００以上、８１００以上、８２００以上、８３００以上、８４００以上、８５００以上、８６００以上、８７００以上、８８００以上、８９００以上、９０００以上、９１００以上、９２００以上、９３００以上、９４００以上、９５００以上、９６００以上、９７００以上、９８００以上、９９００以上、１００００以上、１０１００以上、１０２００以上、１０３００以上、１０４００以上、１０５００以上、１０６００以上、１０７００以上、１０８００以上、１０９００以上、１１０００以上、１１１００以上、１１２００以上、１１３００以上、１１４００以上、１１５００以上、１１６００以上、１１７００以上、１１８００以上、１１９００以上、１２０００以上、１２１００以上、１２２００以上、１２３００以上、１２４００以上、１２５００以上、１２６００以上、１２７００以上、１２８００以上、１２９００以上、１３０００以上、１３１００以上、１３２００以上、１３３００以上、１３４００以上、１３５００以上、１３６００以上、１３７００以上、１３８００以上、１３９００以上、１４０００以上、１４１００以上、１４２００以上、１４３００以上、１４４００以上、１４５００以上、１４６００以上、１４７００以上、１４８００以上、１４９００以上、１５０００以上、１５１００以上、１５２００以上、１５３００以上、１５４００以上、１５５００以上、１５６００以上、または１５７００以上の塩基配列の情報を含むデータベースが使用され得る。
【００６０】
（データベース・参考例６）
さらに、配列番号３３〜６２３６、配列番号７５３３７〜８２７８４、および配列番号１６６１５４〜１６６１８１の塩基配列の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２５以上、３０以上、３５以上、４０以上、４５以上、５０以上、５５以上、６０以上、６５以上、７０以上、７５以上、８０以上、８５以上、９０以上、９５以上、１００以上、２００以上、３００以上、４００以上、５００以上、６００以上、７００以上、８００以上、９００以上、１０００以上、１１００以上、１２００以上、１３００以上、１４００以上、１６００以上、１７００以上、１８００以上、１９００以上、２０００以上、２１００以上、２２００以上、２３００以上、２４００以上、２５００以上、２６００以上、２７００以上、２８００以上、２９００以上、３０００以上、３１００以上、３２００以上、３３００以上、３４００以上、３５００以上、３６００以上、３７００以上、３８００以上、３９００以上、４０００以上、４１００以上、４２００以上、４３００以上、４４００以上、４５００以上、４６００以上、４７００以上、４８００以上、４９００以上、５０００以上、５１００以上、５２００以上、５３００以上、５４００以上、５５００以上、５６００以上、５７００以上、５８００以上、５９００以上、６０００以上、６１００以上、６２００以上、６３００以上、６４００以上、６５００以上、６６００以上、６７００以上、６８００以上、６９００以上、７０００以上、７１００以上、７２００以上、７３００以上、７４００以上、７５００以上、７６００以上、７７００以上、７８００以上、７９００以上、８０００以上、８１００以上、８２００以上、８３００以上、８４００以上、８５００以上、８６００以上、８７００以上、８８００以上、８９００以上、９０００以上、９１００以上、９２００以上、９３００以上、９４００以上、９５００以上、９６００以上、９７００以上、９８００以上、９９００以上、１００００以上、１０１００以上、１０２００以上、１０３００以上、１０４００以上、１０５００以上、１０６００以上、１０７００以上、１０８００以上、１０９００以上、１１０００以上、１１１００以上、１１２００以上、１１３００以上、１１４００以上、１１５００以上、１１６００以上、１１７００以上、１１８００以上、１１９００以上、１２０００以上、１２１００以上、１２２００以上、１２３００以上、１２４００以上、１２５００以上、１２６００以上、１２７００以上、１２８００以上、１２９００以上、１３０００以上、１３１００以上、１３２００以上、１３３００以上、１３４００以上、１３５００以上、または１３６００以上の塩基配列の情報を含むデータベースが使用され得る。
【００６１】
（データベース・参考例７）
さらに、配列番号３３〜６２３６および配列番号１６６１５４〜１６６１８１の塩基配列の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２５以上、３０以上、３５以上、４０以上、４５以上、５０以上、５５以上、６０以上、６５以上、７０以上、７５以上、８０以上、８５以上、９０以上、９５以上、１００以上、２００以上、３００以上、４００以上、５００以上、６００以上、７００以上、８００以上、９００以上、１０００以上、１１００以上、１２００以上、１３００以上、１４００以上、１６００以上、１７００以上、１８００以上、１９００以上、２０００以上、２１００以上、２２００以上、２３００以上、２４００以上、２５００以上、２６００以上、２７００以上、２８００以上、２９００以上、３０００以上、３１００以上、３２００以上、３３００以上、３４００以上、３５００以上、３６００以上、３７００以上、３８００以上、３９００以上、４０００以上、４１００以上、４２００以上、４３００以上、４４００以上、４５００以上、４６００以上、４７００以上、４８００以上、４９００以上、５０００以上、５１００以上、５２００以上、５３００以上、５４００以上、５５００以上、５６００以上、５７００以上、５８００以上、５９００以上、６０００以上、６１００以上、または６２００以上の塩基配列の情報を含むデータベースが使用され得る。
【００６２】
（データベース・参考例８）
さらに、配列番号３３〜６２３６の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２５以上、３０以上、３５以上、４０以上、４５以上、５０以上、５５以上、６０以上、６５以上、７０以上、７５以上、８０以上、８５以上、９０以上、９５以上、１００以上、２００以上、３００以上、４００以上、５００以上、６００以上、７００以上、８００以上、９００以上、１０００以上、１１００以上、１２００以上、１３００以上、１４００以上、１６００以上、１７００以上、１８００以上、１９００以上、２０００以上、２１００以上、２２００以上、２３００以上、２４００以上、２５００以上、２６００以上、２７００以上、２８００以上、２９００以上、３０００以上、３１００以上、３２００以上、３３００以上、３４００以上、３５００以上、３６００以上、３７００以上、３８００以上、３９００以上、４０００以上、４１００以上、４２００以上、４３００以上、４４００以上、４５００以上、４６００以上、４７００以上、４８００以上、４９００以上、５０００以上、５１００以上、５２００以上、５３００以上、５４００以上、５５００以上、５６００以上、５７００以上、５８００以上、５９００以上、６０００以上、６１００以上、または６２００以上の塩基配列の情報を含むデータベースが使用され得る。
【００６３】
（データベース・参考例９）
さらに、配列番号７５３３７〜８２７８４の塩基配列の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２５以上、３０以上、３５以上、４０以上、４５以上、５０以上、５５以上、６０以上、６５以上、７０以上、７５以上、８０以上、８５以上、９０以上、９５以上、１００以上、２００以上、３００以上、４００以上、５００以上、６００以上、７００以上、８００以上、９００以上、１０００以上、１１００以上、１２００以上、１３００以上、１４００以上、１６００以上、１７００以上、１８００以上、１９００以上、２０００以上、２１００以上、２２００以上、２３００以上、２４００以上、２５００以上、２６００以上、２７００以上、２８００以上、２９００以上、３０００以上、３１００以上、３２００以上、３３００以上、３４００以上、３５００以上、３６００以上、３７００以上、３８００以上、３９００以上、４０００以上、４１００以上、４２００以上、４３００以上、４４００以上、４５００以上、４６００以上、４７００以上、４８００以上、４９００以上、５０００以上、５１００以上、５２００以上、５３００以上、５４００以上、５５００以上、５６００以上、５７００以上、５８００以上、５９００以上、６０００以上、６１００以上、６２００以上、６３００以上、６４００以上、６５００以上、６６００以上、６７００以上、６８００以上、６９００以上、７０００以上、７１００以上、７２００以上、７３００以上、または７４００以上の塩基配列の情報を含むデータベースが使用され得る。
【００６４】
（データベース・参考例１０）
さらに、配列番号１６６１５４〜１６６１８１の塩基配列の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２１以上、２２以上、２３以上、２４以上、２５以上、２６以上、または２７以上の塩基配列の情報を含むデータベースが使用され得る。
【００６５】
（データベース・参考例１１）
さらに、配列番号１６６４９０〜１６７０４２の塩基配列の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２５以上、３０以上、３５以上、４０以上、４５以上、５０以上、５５以上、６０以上、６５以上、７０以上、７５以上、８０以上、８５以上、９０以上、９５以上、１００以上、２００以上、３００以上、４００以上、または５００以上の塩基配列の情報を含むデータベースが使用され得る。
【００６６】
（データベース・参考例１２）
さらに、配列番号１７３１２５〜１７４６０３の塩基配列の中から選択される１以上、２以上、３以上、４以上、５以上、６以上、７以上、８以上、９以上、１０以上、１１以上、１２以上、１３以上、１４以上、１５以上、１６以上、１７以上、１８以上、１９以上、２０以上、２５以上、３０以上、３５以上、４０以上、４５以上、５０以上、５５以上、６０以上、６５以上、７０以上、７５以上、８０以上、８５以上、９０以上、９５以上、１００以上、２００以上、３００以上、４００以上、５００以上、６００以上、７００以上、８００以上、９００以上、１０００以上、１１００以上、１２００以上、１３００以上、または１４００以上の塩基配列の情報を含むデータベースが使用され得る。
【００６７】
さらに、本発明の１つの好ましい実施形態では、参照データベースとして下面発酵酵母のゲノムの塩基配列データベースから理論的に翻訳されたアミノ酸配列データベースが用いられ得る。例えば、上記の塩基配列のデータベースに対応するアミノ酸配列データベースが用いられ得る。なお、これらは単なる例示であって、本発明において使用する参照データベースはこれらに限定されるものではない。
【００６８】
本明細書中、「参照データベースに対して参照を行う」とは、参照データベースを検索して、該データベース中に目的のタンパク質の全部または一部のアミノ酸配列または質量パターンに対応する塩基配列またはアミノ酸配列が含まれるか否かを決定することを意味するものとする。通常、このような操作は、コンピュータ上で所定のコンピュータソフトウェアを用いて行われる。検索対象のタンパク質の全部または一部のアミノ酸配列に基づいて検索を行う場合には、例えば、BLAST、FASTA、Smith & Waterman、pep-pat（http://peppat.cbi.pku.edu.cn/）のようなソフトウェアが使用され得るが、これに限定されない。また、検索対象のタンパク質の全部または一部の質量パターンに基づいて検索を行う場合には、例えば、MASCOTのような市販のコンピュータソフトウェアが使用され得るが、これに限定されない。
【００６９】
参照データベースは、コンピュータのハードディスク、ＣＤ−ＲＯＭなどの外部記録媒体、サーバなどの記録媒体に格納された形態で使用され得る。
【００７０】
上記アミノ酸配列に基づく検索においては、変異の存在を考慮して、検索対象の配列に１００％同一のアミノ酸配列のみならず、検索対象の配列に対して６０％以上、好ましくは、７０％以上、より好ましくは、８０％以上、より好ましくは、９０％以上、最も好ましくは、９５％以上、９６％以上、９７％以上、９８％以上、もしくは９９％以上の同一性を有するアミノ酸配列が、データベース中で検索される。このような検索は、BLAST、FASTA、Smith & Waterman等のようなソフトウェアが使用し得る。
【００７１】
ここで、「同一性」については、例えば、「検索対象の配列に少なくとも９５％同一のアミノ酸配列」によって、検索対象のアミノ酸配列が、参照アミノ酸配列中の連続する１００アミノ酸あたり５つまでの不一致（mismatch）を含み得ることを除いて、参照配列に同一である、ということが意図される。「不一致」は、配列中の任意の位置における一つ以上のアミノ酸残基の置換、付加、欠失、および／または挿入によって生じる。
【００７２】
本発明のさらに別の好ましい実施形態では、上記検索結果は、ゲノム配列情報が既知であるS.cerevisiaeのゲノム配列情報と関連づけられた比較データベースを用いて解析される。S.cerevisiaeのＯＲＦのアミノ酸配列との相同性検索により、参照塩基配列において同定されたＯＲＦの機能推定を行うことができる。S.cerevisiaeのゲノム配列情報は、Saccharomyces Genome Database（SGD: http://www.yeastgenome.org/）に登録され、公開されている。
【００７３】
７．同定された遺伝子の機能解析
上記のようにして標的タンパク質をコードする遺伝子を同定することができた場合、そのタンパク質の機能は、例えば、公知のデータベースを検索することによって推定することができる。そのような検索は、配列比較アルゴリズム、例えば、BLASTアルゴリズムなどを用いて行うことができる。すなわち、そのような遺伝子の機能は、検索によって特定された遺伝子と同様の機能であると推定することができる。本明細書において「遺伝子の機能解析を行う」という表現は、公知の情報から機能推定することを含む意味で用いられる。
【００７４】
より具体的には、本発明で用いる参照データベースを用いると、下面発酵酵母のゲノムに含まれるNon-Sc型の遺伝子を特定することができる。そして、このようなNon-Sc型の遺伝子の機能は、比較対象データベース〔例えば、Saccharomyces Genome Database（SGD：http://yeastgenome.org/）に登録されているS.cerevisiaeのＯＲＦのアミノ酸配列および塩基配列、またNational Center of Biotechnology Information（NCBI：http://www.ncbi.nlm.nih.gov/）の非重複（ｎｒ）データベースのアミノ酸配列および塩基配列など〕に対して相同性検索を行うことによってヒットする遺伝子と同様の機能であると推定することができる。
【００７５】
なお、下面発酵酵母ゲノムに含まれる6204個のNon-Sc型ＯＲＦ（配列番号３３−６２３６）および28個のミトコンドリアＯＲＦ（配列番号１６６１５４〜１６６１８１）の推定機能は、明細書の最後部に掲載される。
【００７６】
さらに、標的タンパク質またはそれをコードする遺伝子の機能を確認するためには、上記方法により同定した遺伝子をベクターに挿入して、酵母細胞へ導入して、目的のタンパク質の機能を評価することができる。
【００７７】
このようなベクターは、通常、(a)酵母細胞内で転写可能なプロモーター；(b)該プロモーターにセンス方向またはアンチセンス方向で結合した、上記方法により同定されたポリヌクレオチド（DNA）；及び(c)RNA分子の転写終結およびポリアデニル化に関し、酵母で機能するシグナルを構成要素として含む発現カセットを含むように構成される。
【００７８】
例えば、ビール等の酒類の醸造において、上記同定したタンパク質を高発現させる場合は、上記同定したタンパク質をコードする遺伝子のポリヌクレオチド（DNA）の発現を促進するようにこのポリヌクレオチドを該プロモーターに対してセンス方向に導入する。ビール等の酒類の醸造において、上記同定したタンパク質を抑制する場合は、上記同定したタンパク質をコードする遺伝子のポリヌクレオチド（DNA）の発現を抑制するようにこのポリヌクレオチドを該プロモーターに対してアンチセンス方向に導入する。また、例えば、ビール等の酒類の醸造において、ターゲットとする上記遺伝子（DNA）を破壊することによって、上記DNAの発現または上記タンパク質の発現を抑制することができる。遺伝子の破壊は、ターゲットとする遺伝子における遺伝子産物の発現に関与する領域、例えば、コード領域やプロモーター領域の内部へ単一あるいは複数の塩基を付加あるいは欠失させたり、これらの領域全体を欠失させることにより行うことができる。このような遺伝子破壊の手法は、公知の文献を参照することができる（例えば、Proc. Natl. Acad. Sci. USA, 76, 4951 (1979) 、Methods in Enzymology, 101, 202 (1983)、特開平6-253826号公報など参照）。
【００７９】
酵母に導入する際に用いるベクターとしては、多コピー型（YEp型）、単コピー型（YCp型）、染色体組み込み型（YIp型）のいずれもが利用可能である。例えば、YEp型ベクターとしてはYEp24 (J. R. Broach et al., Experimental Manipulation of Gene Expression, Academic Press, New York, 83 (1983)) 、YCp型ベクターとしてはYCp50 (M. D. Rose et al., Gene, 60, 237 (1987)) 、YIp型ベクターとしてはYIp5 (K. Struhl et al., Proc. Natl. Acad. Sci. USA, 76, 1035 (1979)) が知られており、容易に入手することができる。
【００８０】
酵母での遺伝子発現を調節するためのプロモーター／ターミネーターとしては、例えばグリセルアルデヒド3リン酸デヒドロゲナーゼ遺伝子（TDH3）のプロモーター、3-ホスホグリセレートキナーゼ遺伝子（PGK1）のプロモーターなどが利用可能である。これらの遺伝子はすでにクローニングされており、例えばTuite et al., EMBO J., 1, 603 (1982) に詳細に記載されており、既知の方法により容易に入手することができる。
【００８１】
形質転換の際に用いる選択マーカーとしては、醸造用酵母の場合は栄養要求性マーカーが利用できないので、ジェネチシン耐性遺伝子（G418r）、銅耐性遺伝子（CUP1）（Marin et al., Proc. Natl. Acad. Sci. USA, 81, 337 (1984)）、セルレニン耐性遺伝子（fas2m, PDR4）（それぞれJ. Inokoshi et al., 生化学(Biochemistry), 64, 660 (1992); Hussain et al., Gene, 101, 149 (1991)）などが利用可能である。上記のように構築されるベクターは、宿主酵母に導入される。宿主酵母としては、醸造用に使用可能な任意の酵母、例えばビール，ワイン、清酒等の醸造用酵母等が挙げられる。具体的には、サッカロマイセス（Saccharomyces）属等の酵母が挙げられるが、本発明においては、ビール酵母、例えばサッカロマイセス  パストリアヌス（Saccharomyces pastorianus）バイヘンステファン（Weihenstephan）34/70、サッカロマイセス カールスベルゲンシス（Saccharomyces carlsbergensis）NCYC453、NCYC456等、サッカロマイセス セレビシエ(Saccharomyces cerevisiae) NBRC1951、NBRC1952、NBRC1953、NBRC1954等が使用できる。さらにウイスキー酵母、例えばサッカロマイセス セレビシエNCYC90等、ワイン酵母、例えば協会ぶどう酒用1号、同3号、同4号等、清酒酵母、例えば協会酵母  清酒用7号、同9号等も用いることができるが、これに限定されない。本発明においては、ビール酵母、例えばサッカロマイセス パストリアヌスが好ましく用いられる。
【００８２】
酵母の形質転換方法としては一般に用いられる公知の方法が利用できる。例えば、エレクトロポレーション法“Meth. Enzym., 194, 182 (1990)”、スフェロプラスト法“Proc. Natl. Acad. Sci. USA, 75, 1929 (1978)”、酢酸リチウム法“J. Bacteriology, 153, 163 (1983)、Proc. Natl. Acad. Sci. USA, 75, 1929 (1978)、Methods in Yeast Genetics, 2000 Edition: A Cold Spring Harbor Laboratory Course Manual”などに記載の方法で実施可能であるが、これに限定されない。
【００８３】
より具体的には、宿主酵母を標準酵母栄養培地（例えばYEPD培地“Genetic Engineering. Vo1.1, Plenum Press, New York, 117 (1979)”等）で、OD600nmの値が1〜6となるように培養する。この培養酵母を遠心分離して集め、濃度約1〜2Mのアルカリ金属イオン、好ましくはリチウムイオンで前処理する。この細胞を約30℃で、約60分間静置した後、導入するDNA（約1〜20μg）と混合して約30℃で約60分間静置する。ポリエチレングリコール、好ましくは約4,000ダルトンのポリエチレングリコールを、最終濃度が約20％〜50％となるように加える。約30℃で、約30分間静置した後、この細胞を約42℃で約5分間加熱処理する。好ましくは、この細胞懸濁液を標準酵母栄養培地で洗浄し、新鮮な標準酵母栄養培地に入れて、約30℃で約60分間静置する。その後、選択マーカーとして用いる抗生物質を含む標準寒天培地上に植えつけ、形質転換体を取得する。その他、一般的なクローニング技術に関しては、「モレキュラークローニング第3版」、“Methods in Yeast Genetics、A laboratory manual (Cold Spring Harbor Laboratory Press、Cold Spring Harbor, NY)”等を参照することができる。
【００８４】
目的の遺伝子の発現量の測定は、酵母の培養物から抽出した、目的の遺伝子の産物であるmRNA又はタンパク質を定量することによって可能である。mRNA又はタンパク質の定量は、公知の手法を用いて行うことができる。例えば、mRNAの定量は例えばノーザンハイブリダイゼーションや定量的ＲＴ−ＰＣＲによって、タンパク質の定量は例えばウエスタンブロッティングによって行うことができる(Current Protocols in Molecular Biology, John Wiley & Sons 1994-2003)。
【００８５】
また、ビール醸造試験を行ない、アミノ酸、硫酸イオン、アンモニアなどの資化量、低温耐性、泡もち、ヘイズ生成量、エステル生成量、酵母の凝集性などを評価することによって、標的タンパク質またはそれをコードする遺伝子の機能を確認することができる。
【００８６】
８．形質転換した酵母の評価
基準酵母の特性と被検酵母の特性とを比較することによって、所望の酒類の醸造に好適な被検酵母を選択することができる。この場合、被検酵母または基準酵母としては、例えば、上述したベクターを導入した酵母、上述した遺伝子の発現が制御された酵母、突然変異処理が施された酵母、自然変異した酵母などが使用され得る。突然変異処理は、例えば、紫外線照射法、ＥＭＳ処理法など、公知の方法またはそれに準じる方法によって行うことができる（例えば、大嶋泰治(Yasuji Ohshima)、生物化学実験法39、ｐ67-75、学会出版センターなど参照）。
【００８７】
このように、本発明の方法に従って、ゲノム解読された酵母および該酵母の変異株または酵母バンクより選択された株をそれぞれプロテオーム解析することにより、発現量に変動の認められたタンパク質を同定することが可能である。工業用酵母の野生型株と好適な表現型を有する生産菌株とをプロテオーム解析することにより、目的産物の生産性を向上させる育種のための目的タンパク質の同定に使用できる。具体的には、野生型株とビールの香味成分を大量に生成する変異株とをプロテオーム解析する場合、変異株に対応するスポットを見出し、次いで、各スポット中に含まれるビールの香味成分の増加に寄与するタンパク質および該タンパク質をコードする遺伝子配列を同定することができる。あるいは異なる培養条件下で培養した株をプロテオーム解析することにより、条件間でタンパク量に差のあるスポットを見出し、データベース検索することにより、その培養条件に適応するために必要なタンパク質および該タンパク質をコードする遺伝子塩基配列を同定することができる。
【００８８】
また、本発明の方法を用いて、あるタンパク質をコードする遺伝子の塩基配列だけでなく、そのコード領域の上流域の塩基配列も同時に検索することが可能となる。したがって、例えば醸造用酵母において発現レベルの高いタンパク質をプロテオーム解析により同定することにより、高発現プロモーターとしての機能を有する塩基配列を効率的に選択することができる。また、プロテオーム解析においては、タンパク質の発現量だけでなくタンパク質の修飾も、タンパク質の分離パターンに影響を与えることがある。したがって、本発明の下面発酵酵母の塩基配列情報およびアミノ酸配列情報、または該配列情報が記録された記録媒体を用いた検索により、修飾を受けたタンパク質を効率良く同定することもできる。
【００８９】
実際に、本発明者らは、ビール醸造液中の下面発酵酵母菌体から抽出したタンパク質のプロテオーム解析によって、メチオニン含量の減少に伴って高発現するタンパク質の１つがNon-ScMET17タンパク質であることを確認した。さらに、このNon-ScMET17をコードする遺伝子を同定・単離した。また、このNon-ScMET17遺伝子を遺伝子工学的手法により酵母細胞に導入して高発現させ、この形質転換酵母のビール発酵中の硫化水素生成を大きく減少させた。
【実施例】
【００９０】
以下、実施例によって本発明をより具体的に説明するが、本発明は以下の実施例に限定されるものではない。
【００９１】
実施例  １（ビール発酵中の酵母のプロテオーム解析）
１．ビール発酵試験の実施と酵母菌体のサンプリング
下面発酵酵母サッカロマイセス  パストリアヌス  バイヘンステファン34/70株（pYCGPYNotプラスミド導入株）を用いた発酵試験を以下の条件で行った。
麦汁エキス濃度    12%
麦汁容量          2 L
麦汁溶存酸素濃度  約 8 ppm
発酵温度          15°C一定
酵母投入量        5 g湿酵母菌体/L麦汁
発酵液を定期的にサンプリングし、酵母増殖量（図１を参照）、外観エキス濃度（図２を参照）の経時変化を観察した。また、これと同時に湿菌体重量90 mg相当の酵母菌体を遠心分離（3,000×g、4°C、10分間）により回収した。回収した菌体は数日程度であれば-80°Cで保存可能であった。該凍結菌体は使用直前に室温で融解した。なお、発酵上清も回収し、下記の通りアミノ酸組成の分析を行った。
【００９２】
２．培養上清のアミノ酸分析
培養上清のアミノ酸組成は、高速アミノ酸分析計（日立製作所製の商品名L-8800）及び標準アミノ酸分析カラム（日立製作所社製のP/N855-3506）を用いて分析した。メチオニン濃度の経時変化を図３に示す。
【００９３】
３．電気泳動サンプルの調製
二次元電気泳動のための酵母細胞抽出物を以下の手順で調製した。菌体をlysis buffer（10 mmol/L Tris-HCl pH 8.0、2 mmol/L MgCl2）で1回洗浄した後、0.5 g 乾熱滅菌済みglass beads（425-600 μm、SIGMA社製）、500 μL lysis buffer、Protease Inhibitor Cocktail for Fungal and Yeast Cells（SIGMA社製）（以下、protease inhibitor cocktailと表記する）に懸濁した。これをTWIN 3-28チュプレミキサー（旭テクノグラス社製）を用いて4°Cで45分間攪拌し、菌体を破砕した。この際、試料を15分ごとに2分間氷中に放置した。破砕後、遠心分離（15,000×g、4°C、10分間）により、未破砕菌体や不溶物を除去し、上清画分を回収した。
【００９４】
該上清画分にbenzonase nuclease（Novagen社製）を終濃度が0.25 U/μL試料となるように加え、氷上に30分間放置した。次いで、終濃度が1 mol/Lとなるように5 mol/L NaClを加えて混合し、氷上に5分間放置した後、遠心分離（15,000×g、4°C、5分間）により不溶物を除去して上清画分を回収した。これを二つに分け、それぞれを限外濾過スピンカラム（Millipore社製の商品名Microcon YM-10）に供し、各々100 μL以下になるまで14,000×g、4°Cで濃縮した。Protease inhibitor cocktailを終濃度1%で含む冷超純水400 μLを加えてピペッティングにより混和し、再度各々100 μL以下になるまで14,000×g、4°Cで濃縮した。同様の濃縮操作をさらに3回繰り返して試料の脱塩を行った後、濃縮液を回収した。この濃縮の過程で溶質の一部が沈殿することがあったが、これも共に回収した。回収した試料に4倍量のsample buffer（9.3 M urea、2.3 % (w/v) CHAPS、46.5 mM DTT、0.002% (w/v) BPB）を加えて沈殿を可溶化した後、該試料中のタンパク質濃度を2D-Quant Kit（Amersham Biosciencess社製）を用いて定量した。タンパク質111 μg相当の該試料（通常＜50 μL）と2.5 μL IPG Buffer（pH 4-7、Amersham Biosciences社製）、430 μL sample bufferとを混合し、超純水で総量を500 μLとして電気泳動用試料とした。
【００９５】
４．二次元電気泳動法によるタンパク質の分離
等電点電気泳動法を用い、一次元目の電気泳動を以下のように行った。24 cm Strip Holder（Amersham Biosciences社製）に該電気泳動試料450 μL（タンパク質100 μg相当）をアプライした後、成型済みの乾燥型pHグラジェントストリップゲル（IPG：Immobiline DryStrip、pH 4-7、24cm、Amersham Biosciences社製）をその溶液上に載せて、このIPGストリップ全体を覆うまでImmobiline DryStrip Cover Fluid（Amersham Biosciences社製）をホルダに流し込んだ。このホルダをIPGphor等電点電気泳動装置（Amersham Biosciences社製）のプラットフォームに取り付け、20℃の定温調節下、下記の条件でゲルの再膨潤及び等電点電気泳動を行った。なお、等電点電気泳動中の電流量は50 μA/stripとした。
膨潤：10時間
等電点電気泳動・第一段階：500Vの定電圧で2時間
等電点電気泳動・第二段階：500〜1000Vのグラジェントモードで1時間
等電点電気泳動・第三段階：1000〜8000Vのグラジェントモードで3時間
等電点電気泳動・第四段階：8000Vの定電圧で5.6時間
【００９６】
等電点電気泳動後、IPGストリップゲルをホルダーから取り出し、ゲルに付着した余分なcover fluidを除いて、下記の手順でSDS-PAGEを行った。なお、等電点電気泳動終了後すぐにSDS-PAGEに移れない場合は、IPGストリップゲルを密閉可能なチューブに入れ、-80°Cで数日間保存できた。凍結保存したゲルは室温で解凍後、凍結保存しない場合と同様の手順によりSDS-PAGEに供した。SDS-PAGEを次の手順で行った。IPGストリップゲルを20 mL/stripのSDS equilibration buffer（50 mM Tris-HCl (pH8.8)、6 M 尿素、30% (v/v) グリセロール、2% (w/v) SDS、1% (w/v) DTT、0.002% (w/v) BPB）に浸し、室温で15分間穏やかに振盪して平衡化した。DALT Gel 12.5（Amersham Biosciences社製）をEttan DALT Precast Gel Cassette（Amersham Biosciences社製）にセットし、平衡化したIPGストリップゲルをカソードバッファー（cathode buffer：DALT Buffer Kitに同梱、Amersham Biosciences社製）に浸して数秒間リンスした後、DALT Gel 12.5上面に密着するようにセットした。分子量マーカー（LMW SDS Electrophoresis Calibration Kit、Amersham Biosciences社製）を染み込ませた濾紙片を同様にDALT Gel 12.5上面にセットし、95°C で加熱溶解したシール溶液（sealing solution：DALT Buffer Kitに同梱）で封入した。Ettan DALT電気泳動装置（Amersham Biosciences社製）の泳動漕に規定量のアノードバッファー（anode buffer：DALT Buffer Kitに同梱、Amersham Biosciences社製）を入れた。ユニット中にゲルを挿入した後、陰極側に規定量のカソードバッファー（DALT Buffer Kitに同梱、Amersham Biosciences社製）を注いで電気泳動を開始した。電気泳動は25°Cの定温条件下で、5 W/gelで30分間泳動後、17 W/gelでシール溶液中のBPB色素の先端がゲルの下端に達するまで行った。
【００９７】
５．タンパク質スポットの検出
SDS-PAGE終了後、ゲルをShevchenko, A.らの方法〔Anal. Chem., 68, 850 (1996)〕及びSilver Staining Kit, Protein（Amersham Biosciences社製）添付のプロトコールを参考に、銀染色した。即ち、電気泳動が終了したゲルを固定液（40% (v/v)エタノール、10% (v/v)酢酸）に30分から終夜浸漬した。固定液を交換してさらに30分間置いた後、増感液（30% (v/v)エタノール、0.2% (w/v) Na2S2O3、6.8% (w/v)酢酸ナトリウム、 30分間）、超純水（5分間）×3回、銀反応液（0.25% (w/v) AgNO3、 20分間）、超純水（1分間）×2回の順でゲルを浸し、現像液（2.5% Na2CO3、0.04% (v/v) 37% (w/v)ホルムアルデヒド）に浸してタンパク質スポットを適度な濃さになるまで染色した。その後、ゲルを停止液（1.46% (w/v) EDTA・Na2・2H2O、10分間）、超純水（5分間）×3、保存液（8.7% (w/v)グリセロール、20分間）の順に浸して染色を終了した。なお、染色の各段階はいずれも室温で穏やかに振盪しながら行い、溶液は全て250 mL/gelで用いた。
【００９８】
以上の操作により、菌体から抽出したタンパク質は分離され、スポットとして検出することができた。
【００９９】
６．質量分析用試料の調製
試験発酵開始から8及び32時間後の酵母菌体を用いて行った二次元電気泳動のゲルを比較し、発酵上清中のメチオニンの減少に伴って強度が増すタンパク質スポットを選んで切り出した。スポット中に含まれるタンパク量が質量分析には十分でない場合、同じ泳動試料を供した複数枚のゲルを一画分にまとめた。切り出したゲル片を低吸着チューブに移し、下記の手順でゲル片の脱染及びタンパク質の及びゲル内消化を行った。なお、すぐに処理に移れない場合は固定液（33% (v/v)エタノール、7.5% (v/v)酢酸）をチューブ当たり500 μLで浸漬したまま、数日を上限に4°Cでゲル片を保存した。
【０１００】
ゲル片の脱染は以下の手順で行った。ゲル片に30 mM K3[Fe(CN)6]及び100 mM Na2S2O3の等量混合液100 μLを加え、遮光して室温で10分間振盪した後、液を除去した。続いて超純水500 μLを加えて室温で10分間振盪後、液を除く操作を3回繰り返した。さらに0.1 M NH4HCO3/50% アセトニトリルを300 μL加え、室温で15分間振盪し、液を除く操作を2回繰り返した。
【０１０１】
脱染に続いて、ゲル片に含まれるタンパク質の還元アルキル化を以下の手順で行った。ゲル片に還元バッファー（10 mM DTT、98% 0.2 M Tris、2% EDTA・Na2 (pH8.0)）を200 μL加え、37°Cで1時間インキュベートした。4-ビニルピリジンを4 μL添加後、暗所で1時間放置し、さらに2-メルカプトエタノール4 μL、超純水1 mLを加えてゲル片を30分間静置した後に液を除いた。次いで、超純水1 mLを加えて30分間インキュベートした後に液を除く操作を3回繰り返した後、0.1 M NH4HCO3/50% アセトニトリルを1 mL加えて30分間インキュベートした。上清を除いてゲルを-80°Cで凍結後、SpeedVac真空乾燥機（Thermo electron社製）で2時間乾燥した。
【０１０２】
該乾燥ゲル中のタンパク質の消化を以下の手順で行った。ゲル片に0.1 μg/μL トリプシン溶液5 μLを加え、4℃で10分間静置した。これに消化バッファー（50% 0.2 M NH4HCO3、10% アセトニトリル、0.5% 0.1M CaCl2）を200μL添加し、37°Cで一晩インキュベートした。液相を回収した後、ゲルにさらに0.1% TFA/60 % アセトニトリル 200 μLを加えて３０分間振盪した。この液相と先ほど回収した液相とを合わせて−80℃で凍結し、真空乾燥させた。この凍結乾燥サンプルに2% アセトニトリル/0.1 % TFA 10 μLを加えて10分間振盪した後、5分間の遠心分離により不溶性画分を除いた上清を質量分析用試料に使用した。
【０１０３】
７．液体クロマトグラフィー／タンデム質量分析（LC-MS/MS）による標的タンパク質の質量分析及びアミノ酸配列分析
該質量分析試料をParadigm MS-4高速液体クロマトグラフィー装置（Michrom BioResources社製）及びMagic C18カラム（Michrom BioResources社製）を用いて分離した。移動相は分離状態に応じて〔溶媒A：2% アセトニトリル、0.1% ギ酸；  溶媒B：90% アセトニトリル、0.1% ギ酸〕と〔溶媒Ａ：2% アセトニトリル、0.1% TFA；溶媒Ｂ：90% アセトニトリル、0.1% TFA〕とのいずれかの組を用いた。分離は1.0 μL/分の定流速下、以下のグラジェント条件：
第一段階：溶媒Ｂ5〜65%のグラジェントモードで50分間
第二段階：溶媒Ｂ65〜5%のグラジェントモードで10分間
で行った。
【０１０４】
得られた画分は順次、LCQ Advantage質量分析装置（Thermo electron社製）に供した。
【０１０５】
８．標的タンパク質の同定
上記実施例よりタンパク質スポットに由来するペプチド質量の一覧を取得した。この情報から、ゲノム配列データベースを検索して、該タンパク質に相応するORFをを同定した。タンパク質の同定には、市販のMASCOTプログラム（MATRIX SCIENCE社製）を用いた。検索パラメータのうち、Fixed ModificationsはS-pyridylethyl (C) を、InstrumentはESI-TRAPを選択し、その他のパラメータは全てデフォルトの設定値を用いた。
【０１０６】
９．メチオニン濃度により調節されるタンパク質をコードする遺伝子の検索と同定
メチオニンの減少によって発現量の増加が認められた下面発酵酵母株の菌体由来のタンパク質のうち、分子量50 kDa、pI＝6.0付近に検出されたスポットに含まれるタンパク質を、上記方法により同定した。その結果、該スポットは配列番号1817で表されるNon-Sc型O-アセチルホモセリン-O-アセチルセリンスルフヒドラーゼ（Non-ScMET17）遺伝子にコードされるタンパク質に相応することを示した。また、Non-ScMET17遺伝子のアミノ酸配列は、配列番号１９７０２２で表される。
【０１０７】
この結果より、本発明において構築したゲノム配列データベースを用いての下面発酵酵母より抽出したタンパク質の同定が、そのタンパク質に相応するＯＲＦの塩基配列の検索を可能にすることを示した。さらに、そのＯＲＦの塩基配列と一緒に、そのＯＲＦ上流に位置する塩基配列を検索可能であったので、これらの結果はまた、発酵液中のメチオニン濃度によって発現レベルが調節されるタンパク質を同定することが、メチオニン濃度応答性プロモーターを機能させる塩基配列を効率的に検索可能にすることを示した。
【０１０８】
実施例  ２（酵母へのNon-ScMET17遺伝子の導入および発現）
１．新規Ｏ−アセチルホモセリン−Ｏ−アセチルセリンスルフヒドラーゼ遺伝子（Non-ScMET17）のクローニング
実施例１で同定されたタンパク質の塩基配列情報を基に、それぞれ全長遺伝子を増幅するためのプライマーNon-ScMET17_for（配列番号：１９７０２３）／Non-ScMET17_rv（配列番号：１９７０２４）を設計し、ゲノム解読株サッカロマイセス  パストリアヌス  バイヘンステファン34/70株の染色体DNAを鋳型としたPCRによってNon-ScMET17の全長遺伝子を含むDNA断片（約1.3kb）を取得した。
【０１０９】
増幅されたＤＮＡフラグメントを、TAクローニングによってpCR2.1-TOPOベクター（インビトロジェン社製）に挿入した。Non-ScMET17遺伝子の塩基配列をサンガーの方法 (F. Sanger, Science, 214, 1215, 1981) で分析し、塩基配列を確認した。
【０１１０】
２．Non-ScMET17遺伝子高発現株の構築
上記のNon-ScMET17/pCR2.1-TOPOを制限酵素SacIおよびNotI消化し、Non-ScMET17のタンパク質コード領域全長を含むDNA断片を調製した。この断片を制限酵素SacIおよびNotIにより前処理したpYCGPYNotに連結させ、Non-ScMET17高発現ベクターNon-ScMET17/pYCGPYNotを構築した。pYCGPYNotはYCp型の酵母発現ベクターであり、このベクターに導入された遺伝子はピルビン酸キナーゼ遺伝子PYK1のプロモーターによって高発現される。酵母での選択マーカーとしてジェネティシン耐性遺伝子G418rを、また大腸菌での選択マーカーとしてアンピシリン耐性遺伝子Amprを含んでいる。
【０１１１】
上述の方法で作製した高発現ベクターを用い、特開平07-303475に記載された方法でサッカロマイセス  パストリアヌス  バイヘンステファン34/70株を形質転換した。ジェネチシン300mg/Lを含むYPD平板培地（1%酵母エキス、2%ポリペプトン、2%グルコース、2%寒天）で形質転換体を選択した。
【０１１２】
３．ビール試験醸造における硫化水素生成量の定量
親株ならびに実施例3で得られたＮｏｎ−ＳｃＭＥＴ１７高発現株を用いた発酵試験を以下の条件で行う。
麦汁エキス濃度      12%
麦汁容量            2L
麦汁溶存酸素濃度    約 8ppm 
発酵温度            15℃一定
酵母投入量          5g湿酵母菌体／L麦汁
【０１１３】
発酵液を経時的にサンプリングし、酵母増殖量（OD660）（図４参照）および外観エキス濃度（図５参照）の経時変化を調べたる。発酵中の硫化水素生成の定量は、Takahashiらの手法（Brauwissenschaft. 31. 1 (1978)）を参考にした。検量線を作成するため濃度既知の硫化水素を含む試料を使用して硫化水素を測定し、次いで検出された硫化水素のピーク面積から硫化水素の検量線を作成した。検量線についての試料と同一条件で発酵サンプルを分析して、これに含まれる硫化水素量を、硫化水素のピーク面積と検量線とを比較して決定した。
【０１１４】
表1  発酵終了時における発酵醪の硫化水素量
親株 Non-ScMET17高発現株
H2S(ppb)    22.1           −          
（注）-；検出限界以下（H2Sのピークが検出されず）
【０１１５】
表1より、Non-ScMET17高発現株の発酵終了時における硫化水素生成量は親株の22.1ppbに対してNon-ScMET17高発現株では検出限界以下であった。これらの結果より、Non-ScMET17高発現によって硫化水素生成量が大きく減少することが確認された。
【０１１６】
（参考資料）
本発明者らによる下面発酵酵母ゲノムにコードされる６２０４個のNon-Sc型ＯＲＦ（配列番号３３−６２３６）および２８個のミトコンドリアＯＲＦ（配列番号１６６１５４〜１６６１８１）の推定される機能を以下のリストに示す。
【０１１７】
なお、以下に示すような下面発酵酵母のゲノム配列情報の機能は、配列比較アルゴリズム、例えば、BLASTアルゴリズムを用いて相同性検索によって推定した。例えばBLASTアルゴリズムを使用する場合、比較対象のデータベースを、例えば、Saccharomyces Genome Database（ＳＧＤ：http://genome-www.stanford.edu/Saccharomyces/）に登録されているS.cerevisiaeのＯＲＦのアミノ酸配列もしくは塩基配列、および／または、National Center of Biotechnology Information（ＮＣＢＩ：http://www.ncbi.nlm.nih.gov/）の非重複（ｎｒ）データベースのアミノ酸配列もしくは塩基配列を使用することができる。
【０１１８】
（配列番号３３−６２３６）
33;putative polyol dehydrogenase
34;NAD-dependent (2R,3R)-2,3-butanediol dehydrogenase, a zinc-containing medium-chain alcohol dehydrogenase, produces 2,3-butanediol from acetoin during fermentation and allows using 2,3-butanediol as a carbon source during aerobic growth
35;Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation
36;Acetyl-coA synthetase isoform, expressed during growth on nonfermentable carbon sources and under aerobic conditions
37;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
38;Evolutionarily-conserved tail-anchored outer mitochondrial membrane GTPase which regulates mitochondrial morphology; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; not required for pheromone-induced cell death
39;Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport
40;G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis
41;Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration
42;Hypothetical protein
43;Member of the DRG family of GTP-binding proteins; interacts with translating ribosomes
44;GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2
45;Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
46;Cytoplasmic Glc7p-interacting protein, potential Cdc28p substrate
47;One of two type V myosins; required for mother-specific HO expression, for the bud tip localization of ASH1 and IST2 mRNA; facilitates growth and orientation of ER tubules along with She3p
48;Integral membrane Ca(2+)-ATPase, potential aminophospholipid translocase required to form a specific class of secretory vesicles that accumulate upon actin cytoskeleton disruption; mutation affects maturation of the 18S rRNA
49;Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
50;Putative GDP/GTP exchange factor required for mitotic exit at low temperatures; acts as a guanine nucleotide exchange factor (GEF) for Tem1p, which is a key regulator of mitotic exit; physically associates with Ras2p-GTP
【０１１９】
51;Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt1p, can instead interact with Pmt5p in some conditions; target for new antifungals
52;Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
53;One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status
54;Regulatory subunit A of the heterotrimeric protein phosphatase 2A, which also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and for transcription by RNA polymerase III
55;DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus and mitochondrion
56;Endosomal SNARE related to mammalian syntaxin 8
57;Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine;
58;Subunit of the mitochondrial sorting and assembly machinery (SAM complex); has a role in assembly of the TOM complex, which mediates protein import through the outer membrane; required for normal mitochondrial morphology and inheritance
59;Integral nuclear/ER membrane protein of unknown function, required for normal nuclear envelope morphology and sporulation
60;Mitochondrial protein of unknown function
61;Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles
62;ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall63;Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a dynamic process that is controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate
64;Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles
65;Subunit of heterotrimeric Replication Factor A (RF-A), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
66;N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine
67;Nonessential protein kinase with unknown cellular role
68;Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p
69;Hypothetical protein
70;Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p
71;Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
72;Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
73;Non-essential protein of unknown function; promoter contains several Gcn4p binding elements
74;RNA polymerase II holoenzyme component
75;Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p
76;Mitochondrial ribosomal protein of the large subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
77;Protein kinase, primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene
78;Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
79;Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
80;Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein
81;Protein required, along with Dph1p, Dph2p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Dph2p
82;Peripheral membrane protein that interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane, possibly by influencing its incorporation into lipid rafts
83;Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; induced during respiratory growth and under conditions of oxidative stress
84;Kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation; functionally redundant with Cin8p
85;Type 2C protein phosphatase; dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA checkpoint inactivation
86;Hypothetical protein
87;Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; sas3 gcn5 double mutation confers lethality
88;Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
89;Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for viability in stationary phase
90;High affinity uridine permease, localized to the plasma membrane; not involved in uracil transport
91;20S proteasome beta-type subunit
92;Identified by homology to Ashbya gossypii
93;Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis
94;Mitochondrial ribosomal protein of the large subunit
95;Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport
96;Single-domain racemase, possibly non-specific due to the lack of the second domain, which presumably determines specificity
97;B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
98;GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway
99;Cytoskeletal protein of unknown function; overexpression causes growth arrest
100;Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrially synthesized ATP; required for viability in many common lab strains carrying a mutation in the polymorphic SAL1 gene
【０１２０】
101;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica
102;Hypothetical protein
103;Hypothetical protein
104;Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
105;Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
106;Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
107;Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
108;Hypothetical protein
109;haspin
110;Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis
111;Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements)
112;Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
113;One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p 
114;One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
115;Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
116;Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses
117;Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
118;Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
119;Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
120;Deletion suppressor of mpt5 mutation
121;One of two identical histone H4 proteins (see also HHF2); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
122;One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation
123;Cytoplasmic inorganic pyrophosphatase (PPase), catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
124;Transportin, cytosolic karyopherin beta 2 involved in delivery of heterogeneous nuclear ribonucleoproteins to the nucleoplasm, binds rg-nuclear localization signals on Nab2p and Hrp1p, plays a role in cell-cycle progression
125;Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism
126;UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers
127;Uracil permease, localized to the plasma membrane; expression is tightly regulated by uracil levels and environmental cues
128;Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing
129;Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
130;Hypothetical protein
131;2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis
132;Hypothetical protein
133;Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
134;Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, thus facilitating nuclear export of these proteins; required for viability of npl3 mutants
135;Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
136;Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations
137;Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; has similarity to thioredoxins
138;Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
139;Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating
140;Zeta-crystallin homolog, found in the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin, which has quinone oxidoreductase activity
141;The authentic, non-tagged protein was localized to the mitochondria
142;Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease
143;Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
144;Hypothetical protein
145;Putative plasma membrane protein of unknown function, transcriptionally regulated by Haa1p; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud
146;Hypothetical protein
147;Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
148;Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p
149;2'-O-ribose methyltransferase, methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop
150;Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
【０１２１】
151;Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p
152;Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
153;Amino acid transport protein for valine, leucine, isoleucine, and tyrosine, low-affinity tryptophan and histidine transporter; overexpression confers FK506 resistance
154;Hypothetical protein
155;Small heat shock protein with chaperone activity that is regulated by a heat induced transition from an inactive oligomeric (24-mer) complex to an active dimer; induced by heat, upon entry into stationary phase, and during sporulation
156;Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
157;ATPase required for the release of Sec17p during the 'priming' step in homotypic vacuole fusion and for ER to Golgi transport; homolog of the mammalian NSF
158;Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
159;Transcription factor required for full Ty1 epxression, Ty1-mediated gene activation, and haploid invasive and diploid pseudohyphal growth; TEA/ATTS DNA-binding domain family member
160;Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration
161;Hypothetical protein
162;Plasma membrane protein that may be involved in osmotolerance, localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process
163;Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
164;Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
165;High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Ap; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters
166;Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
167;Hypothetical protein
168;Hypothetical protein
169;Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination and DNA repair
170;Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p) mediates polarized targeting of secretory vesicles to active sites of exocytosis
171;Putative mitochondrial inner membrane transporter, member of the mitochondrial carrier (MCF) family
172;Peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; involved in proteasome-dependent catabolite degradation of FBPase
173;Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
174;Protein with a role in kinetochore function, localizes to the outer kinetochore in a Ctf19p-dependent manner, interacts with Chl4p and Ctf19p
175;Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
176;Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p177;Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids
178;Protein required for translation of the mitochondrial COB mRNA
179;Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation
180;Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm
181;Subunit of an autophagy-specific phosphatidylinositol 3-kinase complex (with Vps34p, Vps15p, and Vps30p) required for organization of a pre-autophagosomal structure; ATG14 transcription is activated by Gln3p during nitrogen starvation
182;High affinity polyamine permease, preferentially uses spermidine over putrescine; expression is down-regulated by osmotic stress; plasma membrane carnitine transporter, also functions as a low-affinity amino acid permease
183;Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p
184;Subunit of the Cdc28 protein kinase, required for mitotic proteolysis, may also be involved in the proteolysis of the G1 cyclins
185;Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); YBR137W is not an essential gene
186;GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase
187;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
188;Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YBR141C; identified by gene-trapping, microarray analysis, and genome-wide homology searching
189;Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
190;Polypeptide release factor involved in translation termination; mutant form acts as a recessive omnipotent suppressor
191;Alcohol dehydrogenase isoenzyme V; involved in ethanol production
192;Mitochondrial ribosomal protein of the small subunit
193;Putative protein of unknown function; YBR147W is not an essential gene; resistant to fluconazole
194;Protein expressed specifically in spores
195;Large subunit of NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; small subunit is unidentified
196;Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
197;mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
198;Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
199;RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
200;Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates
【０１２２】
201;Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
202;Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
203;Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
204;Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
205;Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends
206;Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
207;Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation
208;Short-lived chaperone required for correct maturation of the 20S proteasome; degraded by proteasome upon completion of its assembly; involved in ubiquitin-mediated proteolysis; mutant defective in degradation of short-lived proteins
209;Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
210;Multidrug resistance dityrosine transporter of the major facilitator superfamily, essential for spore wall synthesis, facilitates the translocation of bisformyl dityrosine through the prospore membrane
211;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YBR187W is an essential gene
212;Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
213;Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
214;Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
215;Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
216;Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
217;Endoplasmic reticulum membrane protein, required for ER-associated protein degradation, involved in the retrograde transport of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p
218;Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
219;Protein required for wild-type resistance to the antifungal drug ciclopirox olamine; not related to the COS family of subtelomerically-encoded proteins
220;Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
221;Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis
222;Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme; also involved in the expression of PAPS reductase and sulfite reductase
223;One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis
224;Ubiquitin-like modifier, conjugated via an isopeptide bond to a lysine residue of Atg5p by the E1 enzyme, Atg7p, and the E2 enzyme, Atg10p, a step that is essential for autophagy
225;Putative protein of unknown function; YBR220C is not an essential gene
226;E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria
227;Peroxisomal AMP-binding protein, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase
228;Hypothetical protein
229;RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length
230;Essential protein, component of the DASH complex; involved in spindle integrity and kinetochore function; interacts with Duo1p and Dam1p; localizes to intranuclear spindles and kinetochore
231;Essential subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
232;Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene
233;RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
234;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR239C is not an essential gene
235;Zinc finger protein of the Zn(II)2Cys6 type, probable transcriptional activator of thiamine biosynthetic genes
236;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene
237;Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress
238;Hypothetical protein
239;Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
240;3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine
241;Mitochondrial ribosomal protein of the small subunit
242;One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
243;Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
244;Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
245;Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
246;Protein of unknown function involved in bud growth in the mitotic signaling network; proposed negative regulator of Swe1p and Gin4p; contains dispersed C2H2 zinc finger domains
247;Mitochondrial ribosomal protein of the large subunit
248;Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR271W is not as essential gene
249;Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
250;Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
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251;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR281C is not an essential gene
252;Mitochondrial ribosomal protein of the large subunit
253;Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential
254;Putative protein of unknown function; YBR284W is not an essential gene; null mutant exhibits decreased resistance to rapamycin and wortmannin
255;Putative protein of unknown function; YBR287W is not an essential gene and deletion of the YBR287W leads to poor growth on glucose-minimal medium at 15C
256;Protein of unknown function; mutation results in a zinc sensitive phenotype
257;Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
258;Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
259;Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p
260;Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine
261;S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint
262;Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
263;Hypothetical protein, has similarity to proteins in S. pombe, C. elegans, D. melanogaster.
264;Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins
265;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
266;Transcription factor required for induction of KAR3 and CIK1 during mating, also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone
267;Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
268;Protein involved in control of cell wall structure and stress response; inhibits Cbk1p protein kinase activity; overproduction confers resistance to cell-wall degrading enzymes
269;Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p
270;Hypothetical protein
271;Hypothetical protein
272;Subunit, with Yme1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; required for growth of cells lacking the mitochondrial genome
273;Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources
274;Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions
275;Protein required for the breakdown of autophagic vesicles in the vacuole during autophagy, putative integral membrane protein that localizes to vacuolar membranes and punctate structures attached to the vacuole
276;Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
277;Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
278;Essential protein involved in rRNA processing and ribosome biogenesis
279;Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis
280;Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170
281;[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate
282;Protein of unknown function, involved in the integration of lipid signaling pathways with cellular homeostasis
283;Beta-isopropylmalate dehydrogenase, catalyzes the third step in the leucine biosynthesis pathway
284;Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
285;Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion
286;Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding
287;Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro
288;SaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGA histone acetyltransferase complex
289;Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
290;Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface
291;Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
292;Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER
293;Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM
294;Mitochondrial ribosomal protein of the large subunit
295;Protein of unknown function, has sequence and structural similarity to flavodoxins; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
296;Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors
297;Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
298;Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress
299;Acetate transporter required for normal sporulation
300;Putative ATP-dependent permease of the ABC transporter family of proteins
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301;Protein required for respiratory growth and stability of the mitochondrial genome
302;Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family
303;Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
304;Mitochondrial asparaginyl-tRNA synthetase
305;Small single-membrane span proteolipid that functions as a regulatory subunit of the plasma membrane H(+)-ATPase Pma1p, forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines
306;Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation
307;Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
308;Protein with a potential role in actin cytoskeletal organization; overexpression suppresses a pfy1 (profilin) null mutation
309;Subunit of the Set3C deacetylase complex; putative DNA-binding protein
310;Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway
311;Hypothetical protein
312;Hypothetical protein
313;Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
314;Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex
315;Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p
316;Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p
317;ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
318;One of five related septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p, Shs1p) that form a cortical filamentous collar at the mother-bud neck which is necessary for normal morphogenesis and cytokinesis
319;Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division
320;Hypothetical protein
321;Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane
322;Protein kinase with a possible role in MAP kinase signaling in the pheromone response pathway
323;Protein with similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; computational analysis of large-scale protein-protein interaction data suggests a possible role in rRNA processing
324;Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
325;Hypothetical protein
326;Cytoplasmic nucleoporin required for polyadenylated RNA export but not for protein import; component of Nup82p nuclear pore subcomplex; contains a nuclear export signal
327;Phorphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p
328;Protein of unknown function; has similarity to mammalian reticulon proteins; member of the RTNLA (reticulon-like A) subfamily
329;Mitochondrial ribosomal protein of the large subunit
330;Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA
331;Prenyltransferase, required for cell viability
332;ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p
333;Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis
334;Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Bp and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable
335;Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase, inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has similarity to Stf1p and both Inh1p and Stf1p exhibit the potential to form coiled-coil structures
336;Hypothetical protein
337;Hypothetical protein
338;Hypothetical protein
339;RING finger protein that interacts with the arginine methyltransferase Hmt1p; may regulate methylation of Npl3p, which modulates Npl3p function in mRNA processing and export; has similarity to Air1p
340;D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
341;Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus
342;Essential nuclear protein, involved in the oxidative stress response
343;Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
344;DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
345;Epsin-like protein involved in endocytosis and actin patch assembly and functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus
346;Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping, coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation
347;B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation
348;Component of the small (ribosomal) subunit (SSU) processosome required for pre-18S rRNa processing; essential nucleolar protein that, when overproduced, disrupts silencing
349;RNA polymerase III subunit C53
350;Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p
【０１２５】
351;Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface
352;Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
353;RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
354;ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf1p
355;Rho GDP dissociation inhibitor involved in the localization and regulation of Cdc42p
356;Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Ap and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable
357;Hypothetical protein
358;Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
359;Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein
360;ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome
361;Protein of unknown function, localized to the vacuolar outer membrane
362;Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
363;Hypothetical protein
364;Frataxin, regulates mitochondrial iron accumulation; interacts with Isu1p which promotes Fe-S cluster assembly; interacts with electron transport chain components and may influence respiration; human homolog involved in Friedrich's ataxia
365;Hypothetical protein
366;Protein required for transport of aminopeptidase I (Lap4p) through the cytoplasm-to-vacuole targeting pathway; binds phosphatidylinositol-3-phosphate, involved in localization of membranes to the preautophagosome, potential Cdc28p substrate
367;2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
368;Protein involved in rRNA processing; component of the exosome 3->5 exonuclea
se complex with Rrp4p, Rrp41p, Rrp43p and Dis3p
369;Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p
370;Nuclear protein that plays a role in the function of the Smc5p-Rhc18p complex
371;Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
372;Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair
373;ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress
374;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
375;Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt2p, can instead interact with Pmt3p in some conditions; target for new antifungals
376;Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
377;UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
378;Protein of unknown function; interacts with meiotic division protein Csm1p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery, potential Cdc28p substrate
379;Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p
380;Hypothetical protein
381;Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain
382;Component of the TREX complex required for nuclear mRNA export; DEAD-box RNA helicase involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56
383;Ribosomal protein P1 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
384;Cytoplasmic malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle
385;Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
386;Protein of unknown function, may be involved in chromatin silencing
387;E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on L4 and L79), contains RING finger domain
388;Endoplasmic reticulum transmembrane protein, homolog of human BAP31 protein
389;Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p
390;Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
391;Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
392;Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Ap and has similarity to rat S29 and E. coli S14 ribosomal proteins
393;Protein required for processing of 20S pre-rRNA in the cytoplasm, associates with pre-40S ribosomal particles
394;Hypothetical protein
395;GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
396;Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
397;1-acyl-sn-gylcerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; located in lipid particles and endoplasmic reticulum
398;Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p
399;Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization
400;Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes
【０１２６】
401;Mitochondrial ribosomal protein of the small subunit
402;Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
403;Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
404;Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
405;Dual-specificity kinase required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, and checkpoint protein Mad1p
406;Hypothetical protein
407;Protein of unknown function, potentially phosphorylated by Cdc28p
408;Homolog of Gpm1p phosphoglycerate mutase which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
409;Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses
410;Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
411;Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
412;DDK (Dbf4-dependent kinase) catalytic subunit required for firing origins and replication fork progression in mitosis through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression
413;Enoyl reductase that catalyzes the last step in each cycle of very long chain fatty acid elongation, localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions, coimmunoprecipitates with elongases Fen1p and Sur4p
414;Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
415;Ring finger protein involved in the DNA damage response with possible recombination role; genetically identified by synthetic lethality with SGS1 (DNA helicase) and TOP3 (DNA topoisomerase); sporulation role; interacts with Slx8p and Lin1p
416;Hypothetical protein
417;One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
418;Type 2C protein phosphatase (PP2C); inactivates the osmosensing MAPK cascade by dephosphorylating Hog1p; mutation delays mitochondrial inheritance; deletion reveals defects in precursor tRNA splicing, sporulation and cell separation
419;Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
420;Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
421;Essential protein required for sister chromatid cohesion in mitosis and meiosis; subunit of the cohesin complex; expression is cell cycle regulated and peaks in S phase
422;Protein related to mammalian high mobility group proteins; likely component of the INO80 complex, which is an ATP-dependent chromatin-remodeling complex
423;Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p
424;Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
425;ABC transporter protein involved in multidrug resistance and resistance to singlet oxygen species
426;Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
427;Inositol hexaphosphate kinase, phosphorylates inositol hexakisphosphate (InsP6) to diphosphoinositol polyphosphates, required for proper vacuole morphology and involved in salt stress response, contains two leucine heptad repeats
428;Hypothetical protein
429;Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
430;Protein of unknown function that may be involved in microtubule organization; high-copy suppressor of CIK1 deletion
431;Hypothetical protein
432;Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; potentially phosphorylated by Cdc28p
433;Protein involved in transcription-coupled repair nucleotide excision repair of UV-induced DNA lesions; homolog of human CSA protein
434;Protein of unknown function with similarity to members of a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
435;Protein that localizes primarily to the plasma membrane, also found at the nuclear envelope; has similarity to Hsp30p and Yro2p, which are induced during heat shock
436;Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
437;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
438;3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine
439;Protein of unconfirmed function, plays an indirect role in endocytic membrane trafficking, member of a family of enoyl-CoA hydratase/isomerases
440;Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
441;Transcriptional repressor that recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response
442;Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; localizes to the mitochondrial inner membrane; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
443;RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription
444;Amino acid permease involved in the uptake of cysteine, leucine, isoleucine and valine
445;Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; activity inhibited by Cu2+, Zn2+, Fe2+, Fe3+ and sulfhydryl-specific reagents
446;Hypothetical protein
447;Ubiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p, Cdc53p, and an F-box protein, forms a ubiquitin-protein ligase called the SCF complex which regulates cell cycle progression by targeting key substrates for degradation
448;Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1
449;Lectin; soluble lumenal ER protein; member of the OS-9 protein family; similar to mannose-6-phosphate receptors (MPRs); serves as a receptor that recognizes misfolded N-glycosylated proteins and participates in their targeting to ERAD
450;Protein with lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli; role in yeast lipid degradation is unclear
【０１２７】
451;Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
452;Hypothetical protein
453;Hypothetical protein
454;Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
455;Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
456;Ubiquitin hydrolase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
457;The authentic, non-tagged protein was localized to the mitochondria
458;Polyamine acetyltransferase; acetylates polyamines such as putrescine, spermidine and spermine; may be involved in transcription and/or DNA replication via regulation of levels of polyamines bound to chromosomal DNA
459;One of 11 subunits of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p
460;Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway
461;Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
462;Protein of unassigned function involved in mutation suppression, important for error-free repair of spontaneous and induced DNA lesions to protect the genome from mutation; associates with Shu1p, Psy3p, and Csm2p
463;Chaperone that specifically facilitates the assembly of cytochrome c oxidase, located in the mitochondrial inner membrane
464;Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation; homolog of the Chlamydomonas reinhardtii REX1-S protein which is involved in DNA repair
465;Transcription factor required for the synthesis of the glycolytic enzyme pyruvate decarboxylase, required for high level expression of both the THI and the PDC genes
466;Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
467;Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
468;RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
469;Hypothetical protein
470;Hypothetical protein
471;Essential iron-sulfur protein required for ribosome biogenesis and translation initiation; facilitates binding of a multifactor complex (MFC) of translation initiation factors to the small ribosomal subunit; predicted ABC family ATPase
472;Transcriptional factor, involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1
473;Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentiall
y phosphorylated by Cdc28p
474;14-3-3 protein, minor isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others
475;Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p
476;Scaffold protein that, in response to pheromone, shuttles from the nucleus to the plasma membrane and assembles kinases Ste11p, Ste7p, and Fus3p into a specific signaling complex; active oligomeric form interacts with Ste4p-Ste18p complex
477;Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
478;multispanning membrane protein
479;Putative alanine transaminase (glutamic pyruvic transaminase)
480;Putative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins
481;Mitochondrial ribosomal protein of the large subunit
482;Hypothetical protein
483;Palmitoyltransferase that acts on the SNAREs Snc1p, Syn8p, Tlg1p and likely on all SNAREs; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
484;Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin; similar to human cystic fibrosis protein CFTR
485;Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
486;Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
487;GPI-anchored aspartyl protease (yapsin) involved in protein processing; shares functions with Yap3p and Kex2p
488;Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
489;Dihydrolipoyl transsuccinylase, a component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA
490;Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis
491;Highly-acidic cytoplasmic RWD domain-containing protein of unknown function, sensitive to proteolysis, N-terminal region has high content of acidic amino acid residues, putative IUP (intrinsically unstructured protein)
492;Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
493;Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A
494;Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
495;Nuclear pore-associated protein, forms a complex with Thp1p that is involved in transcription and in mRNA export from the nucleus
496;Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
497;interacts with PP2C
498;Protein involved in the HOG (high osmolarity glycerol) pathway, negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex, found in the nucleus and cytoplasm, contains an SH3 domain that binds Pbs2p
499;Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p
500;Sm-like protein involved in docking and fusion of exocytic vesicles through binding to assembled SNARE complexes at the membrane; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
【０１２８】
501;Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA
502;Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
503;Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle
504;Protein that binds Sin3p in a two-hybrid assay
505;Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
506;Small cytosolic stress-induced chaperone that forms barrel-shaped oligomers and suppresses the aggregation of non-native proteins; oligomer dissociation is not required for function; involved in cytoskeleton reorganization after heat shock
507;Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype
508;Protein involved in regulation of arginine-responsive and Mcm1p-dependent genes; has a dual-specificity inositol polyphosphate kinase activity required for regulation of phosphate- and nitrogen-responsive genes
509;Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
510;Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response
511;Putative protein kinase and subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX
512;Putative membrane protein of unknown function involved in Mn2+ homeostasis; mutants display actin and general growth defects, heterogeneous cell cycle arrests, and pleiotropic defects in cell cycle progression and organelle distribution
513;Hypothetical protein
514;Hypothetical protein
515;Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
516;Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family
517;Subunit of the nuclear pore complex (NPC) that localizes exclusively to the cytoplasmic side; involved in RNA export, most likely at a terminal step; interacts with Gle1p
518;DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; presumed RNA helicase due to DEAD-box motif
519;Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
520;Component of Dam1p complex, important for spindle and kinetochore integrity; localized to nuclear side of spindle pole body and along mitotic spindle
521;Protein of unknown function, contains a putative RNA recognition motif, deletion results in short telomeres; similar to Est1p, may be partially redundant with Est1p for telomere maintenance
522;Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p
523;Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
524;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
525;Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
526;Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in maintenance of actin cytoskeleton; homolog to Drosophila melanogaster and mouse tailless complex polypeptide
527;Sterol regulatory element binding protein, induces transcription of sterol transport and biosynthetic genes; involved in the anaerobic induction of DAN/TIR mannoproteins and seripauperins; binucleate zinc cluster protein; Ecm22p homolog
528;Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock
529;Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p, involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER
530;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
531;One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation 
532;One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p 
533;Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence
534;mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p)
535;Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase
536;Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
537;Protein of unknown function; has similarity to mammalian reticulon proteins; member of the RTNLA (reticulon-like A) subfamily
538;Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
539;Riboflavin kinase, phosphorylates riboflavin to form riboflavin monophosphate (FMN), which is a necessary cofactor for many enzymes; localizes to microsomes and to the mitochondrial inner membrane
540;Mitochondrial ribosomal protein of the large subunit
541;Putative amidase
542;RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
543;Peroxisomal membrane signal receptor for C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import, tetratricopeptide repeat protein, also involved in PTS1-independent import
544;One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
545;Hypothetical protein
546;Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype
547;Zinc-finger DNA-binding protein, involved in regulating expression of the methionine biosynthetic genes, similar to Met31p
548;Cytosolic protein required for sporulation; also required for the ubiquitination of the gluconeogenetic enzyme fructose-1,6-bisphosphatase, which is degraded rapidly after the switch from gluconeogenesis to glycolysis
549;Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded matrix proteins; component of the mitochondrial proteolysis system
550;Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
【０１２９】
551;Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
552;Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats
553;RING finger peroxisomal membrane peroxin required for peroxisomal matrix protein import, interacts with Pex12p, links ubiquitin-conjugating Pex4p to protein import machinery; mutations in human homolog cause a variety of peroxisomal disorders
554;Hypothetical protein
555;Mitochondrial tryptophanyl-tRNA synthetase
556;Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
557;Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
558;Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation
559;Ribonuclease H2 subunit, required for RNase H2 activity
560;Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate
561;inositol monophosphatase
562;Essential protein of unknown function; interacts with Nse4p, which is a component of the Smc5/6 DNA repair complex
563;Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition564;Signal recognition particle (SRP) receptor - alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with SRP102p
565;Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda3p; Ploidy-related
566;Protein involved in homologous recombination in mitochondria and in transcription regulation in nucleus; binds to activation domains of acidic activators; required for recombination-dependent mtDNA partitioning
567;Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein)
568;Essential protein possibly involved in secretion; multicopy suppressor of sensitivity to Brefeldin A
569;RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
570;ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
571;One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p
572;Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER
573;Hypothetical protein
574;RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
575;Hypothetical protein
576;Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
577;Hypothetical protein
578;Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
579;Essential protein, component of the DASH complex; involved in spindle integrity and kinetochore function; interacts with Duo1p and Dam1p; localizes to intranuclear spindles and kinetochore
580;Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric state of ATP synthase
581;Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase
582;ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
583;Karyopherin involved in nuclear import and export; shown to be responsible for nuclear import of replication protein A and for export of several proteins including Swi6p, Far1p, and Pho4p; cargo dissociation involves binding to RanGTP
584;Hypothetical protein
585;Mitochondrial ribosomal protein of the small subunit
586;Hypothetical protein
587;Protein required for cell viability
588;Cytoplasmic arginyl-tRNA synthetase
589;Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions
590;Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis
591;Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein
592;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud neck; potential Cdc28p substrate
593;Putative GPI-anchored aspartic protease, located in the cytoplasm and endoplasmic reticulum
594;Hypothetical protein
595;Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress
596;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
597;Protein involved in mating-type locus silencing, interacts with Sir2p; probably functions to recruit or stabilize Sir proteins
598;Pre-mRNA splicing factor, important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats
599;Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
600;2-methylbutyraldehyde reductase, may be involved in isoleucine catabolism
【０１３０】
601;Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
602;Non-essential protein of unknown function involved in vacuolar protein sorting; belongs to a family of cytosolic Golgi-associated proteins suggesting that it may play a role in secretion; also detected in the nucleus
603;N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
604;Hypothetical protein
605;Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
606;Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
607;GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth
608;Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36.
609;Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway
610;Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
611;Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; member of the TC 9.B.33 YaaH family of putative transporters
612;Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin
613;Helix-hairpin-helix protein, involved in DNA repair and replication fork stability; functions as an endonuclease in complex with Mms4p; interacts with Rad54p
614;Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
615;One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
616;Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
617;Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
618;Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
619;RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p
620;Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
621;Protein required for cell viability
622;Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
623;Hypothetical protein
624;Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins
625;Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; forms a complex with Snx4p and Atg20p
626;Hypothetical protein
627;Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation
628;Carboxyl methyl transferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
629;Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
630;Protein involved in rDNA silencing; positively charged coiled-coil protein with limited similarity to myosin
631;Apparent pseudogene, not transcribed or translated under normal conditions; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity
632;Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
633;One of two homeobox transcriptional repressors (see also Yox1p), that bind to Mcm1p and to early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval
634;Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer
635;Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock
636;E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators
637;Mitochondrial ribosomal protein of the large subunit
638;Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
639;Protein of unknown function; outer membrane component of the mitochondrial fusion machinery; Ugo1p bind directly to Fzo1p and Mgm1p and thereby link these two GTPases during mitochondrial fusion
640;Splicing factor, component of the U4/U6-U5 snRNP complex
641;AMP-activated serine/threonine protein kinase found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis
642;Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation
643;Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
644;Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
645;Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p
646;Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation
647;Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA
648;Myo-inositol transporter with strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p 
649;Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP
650;Protein required for partitioning of the 2-micron plasmid
【０１３１】
651;Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition
652;Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p
653;High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids
654;Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics
655;Hypothetical protein
656;RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts
657;Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation
658;Protein disulfide isomerase of the endoplasmic reticulum lumen, function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
659;Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking
660;ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
661;ORF, Uncharacterized
662;Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
663;Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
664;Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
665;Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp32p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer
666;D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
667;Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
668;Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
669;Vacuolar proteinase B (yscB), a serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation
670;Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates
671;Hypothetical protein
672;Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
673;Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins
674;Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis675;Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
676;Hypothetical protein
677;Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
678;Hypothetical protein
679;Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration
680;Protein with ubiquitin-like N terminus, recognizes and binds damaged DNA (with Rad4p) during nucleotide excision repair; regulates Rad4p levels, subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human HR23A and HR23B proteins
681;Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
682;Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Anb1p; undergoes an essential hypusination modification; expressed under aerobic conditions
683;Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
684;P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
685;Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; has similarity to pyridoxal kinases
686;Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis
687;RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein
688;Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration
689;Orotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound
690;Mitochondrial intermembrane space protein, forms a complex with Mrs11p/Tim10p that mediates import and insertion of a subset of polytopic inner membrane proteins; may prevent aggregation of incoming proteins in a chaperone-like manner
691;Hypothetical protein with low sequence identity to Pdc1p
692;SUMO ligase involved in chromosomal organization and DNA repair; forms a complex with Smc5p, Rhc18p, and Nse1p; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
693;Esa1p-associated factor, subunit of the NuA4 acetyltransferase complex
694;Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; nearly identical to PMP1
695;Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery
696;Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies
697;Phosphorylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
698;Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
699;Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
700;Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
【０１３２】
701;Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
702;Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential
703;Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
704;Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking
705;20S proteasome beta-type subunit; localizes to the nucleus throughout the cell cycle
706;Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX
707;Long chain fatty acyl-CoA synthetase; accepts a wider range of acyl chain lengths than Faa1p, preferring C9:0-C13:0; involved in the activation of endogenous pools of fatty acids
708;Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
709;Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes
710;Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
711;Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
712;Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
713;Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control714;Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
715;Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis
716;Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
717;Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
718;Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline
719;One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain
720;Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B'
721;GTPase of the Ypt/Rab family, very similar to Ypt32p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi
722;Cytoplasmic protein of unknown function, transcription is induced under conditions of zinc deficiency
723;Hypothetical protein
724;ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
725;Protein of unknown function, expression is induced by low phosphate levels and by inactivation of Pho85p
726;Essential protein of unknown function; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
727;Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source
728;Protein of unknown function, has similarity to endonuclease Rth1p; potentially phosphorylated by Cdc28p
729;Peptide methionine sulfoxide reductase, reverses the oxidation of methionine residues; involved in oxidative damage repair, providing resistance to oxidative stress and regulation of lifespan
730;S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
731;Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p
732;Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
733;Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system
734;Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
735;Protein required for mitochondrial iron-sulfur cluster biosynthesis
736;Hypothetical protein
737;Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
738;Hypothetical protein
739;ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
740;Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro
741;Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding
742;Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
743;One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition
744;Protein of unknown function; overproduction suppresses the transcriptional defect caused by an hpr1 mutation
745;Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
746;Hypothetical protein
747;Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
748;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
749;Vacuolar transporter chaperon (VTC) involved in distributing V-ATPase and other membrane proteins; together with other VTC proteins, forms a heterotetrameric complex that associates with the SNARE Nyv1p and the V0 sector of the V-ATPase
750;Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
【０１３３】
751;Metallopeptidase, localized to the mitochondrial matrix
752;Hypothetical protein
753;The authentic, non-tagged protein was localized to the mitochondria
754;Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
755;Hypothetical protein
756;Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex; homologous to Sbh2p
757;Cobalamin-independent methionine synthase, involved in amino acid biosynthesis; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
758;Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions
759;Beta subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
760;Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
761;Sporulation-specific activator of Chs3p (chitin synthase III), required for the synthesis of the chitosan layer of ascospores; has similarity to Skt5p, which activates Chs3p during vegetative growth; transcriptionally induced at alkaline pH
762;Ubiquitin-specific protease that cleaves ubiquitin-protein fusions
763;Ubiquitin-conjugating enzyme involved in ER-associated protein degradation; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway
764;Protein that may have a role in targeting of plasma membrane [H+]ATPase (Pma1p) to the plasma membrane, as suggested by analysis of genetic interactions
765;Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein
766;Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation
767;Protein with a role in regulation of Ty1 transposition
768;Abundant subunit of the nuclear pore complex (NPC), present on both sides of the NPC, has similarity to Nup170p
769;Component of the nuclear pore complex required for polyadenylated RNA export but not for protein import, homologous to S. pombe Rae1p
770;Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene
771;Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
772;DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair
773;Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
774;Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain
775;Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
776;Protein containing a RING finger domain that forms a complex with Hex3p; mutant phenotypes and genetic interactions suggest a possible role in resolving recombination intermediates during DNA replication or repair
777;Vacuolar transporter, exports aspartate and glutamate from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
778;Integral ER membrane protein that regulates phospholipid metabolism via an interaction with the FFAT motif of Opi1p, also involved in telomeric silencing, disruption causes inositol auxotrophy above 34 degrees C, VAP homolog
779;ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p
780;Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of the HOPS complex subunit, Vps41p; shares overlapping essential functions with Hrr25p
781;Daughter cell-specific protein, may participate in pathways regulating cell wall metabolism; deletion affects cell separation after division and sensitivity to drugs targeted against the cell wall
782;Ubiquitin-protein ligase involved in ubiquitin-mediated protein degradation; plays a role in heat shock element (HSE)-mediated gene expression and multivesicular body sorting; contains a hect (homologous to E6-AP carboxyl terminus) domain
783;Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
784;Hypothetical protein
785;Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similarity to rat S26 ribosomal protein
786;Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions
787;GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
788;Hypothetical protein
789;Hypothetical protein
790;Hypothetical protein
791;Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
792;DNA damage-inducible v-SNARE binding protein, contains a ubiquitin-associated (UBA) domain, may act as a negative regulator of constitutive exocytosis, may play a role in S-phase checkpoint control
793;Putative ubiquitin-specific protease that does not associate with the proteasome
794;High affinity iron permease involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron
795;TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
796;TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
797;TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
798;Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth
799;GPI-anchored, serine/threonine rich cell wall protein of unknown function; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p
800;Specific translational activator for the COX3 mRNA that acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
【０１３４】
801;Translocase of the mitochondrial inner membrane, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; null is respiratory deficient
802;Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence
803;Hypothetical protein
804;Protein involved in negative regulation of transcription, exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins
805;Hypothetical protein
806;Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes
807;Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
808;Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations
809;ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
810;5' to 3' DNA helicase, involved in nucleotide excision repair and transcription; subunit of RNA polymerase II transcription initiation factor TFIIH; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein
811;RNA-dependent ATPase RNA helicase involved in the facilitation and disruption of snRNA interactions, required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis
812;Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage
813;Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
814;DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
815;14-3-3 protein, major isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others
816;Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
817;Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
818;Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO3
819;Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13
820;Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
821;The authentic, non-tagged protein was localized to the mitochondria
822;Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
823;Putative protein of unknown function; YFL040W is not an essential gene
824;Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules
825;Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition
826;Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
827;Alanine : glyoxylate aminotransferase, catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine : glyoxylate aminotransferases
828;Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
829;Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation; putative ABC ATPase; interacts with Ssn2p, Ssn3p, and Ssn8p
830;GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport
831;Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells
832;GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules833;Component of NuA4, which is an essential histone H4/H2A acetyltransferase complex; homologous to Drosophila Enhancer of Polycomb
834;Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
835;Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes
836;Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
837;Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
838;Protein involved in folding of mitochondrially synthesized proteins in the mitochondrial matrix; localizes to the mitochondrial inner membrane; member of the DnaJ family of molecular chaperones
839;Plasma membrane localized protein that protects membranes from desiccation; induced by heat shock, oxidative stress, osmostress, stationary phase entry, glucose depletion, oleate and alcohol; regulated by the HOG and Ras-Pka pathways
840;WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes Gi phase growth arrest and clonal death that is suppressed by overexpression of MCA1
841;Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
842;Secretory vesicle-associated Rab GTPase essential for exocytosis; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
843;Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
844;Non-essential tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm
845;Component of the nuclear pore complex, required for nuclear pore formation; forms a subcomplex with Nsp1p, Nup57p, and Nup49p
846;Inhibitor of the type I protein phosphatase Glc7p, which is involved in regulation of a variety of metabolic processes; overproduction causes decreased cellular content of glycogen
847;Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates
848;Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
849;Hypothetical protein
850;Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
【０１３５】
851;Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YFR009W; identified by expression profiling and mass spectrometry
852;Putative protein of unknown function; localizes to the mitochondrion; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR011C is not an essential gene
853;Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing
854;Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase
855;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR017C is not an essential gene
856;1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
857;Phosphatidylinositol 3,5-bisphosphate-binding protein of the vacuolar membrane, predicted to fold as a seven-bladed beta-propeller; required for recycling of Atg9p through the pre-autophagosomal structure
858;Protein that binds to Rsp5p, which is a hect-type ubiquitin ligase, via its 2 PY motifs; has similarity to Rod1p; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant
859;Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation, similar to Mip6p
860;Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
861;Acetyltransferase required for the establishment of sister chromatid cohesion during DNA replication, but not for its maintenance during G2 and M phases; also required for postreplicative double-strand break repair; interacts with Chl1p
862;Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit
863;Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
864;Subunit alpha of assimilatory sulfite reductase, which is responsible for the conversion of sulfite into sulfide
865;Component of the condensin complex, essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA, possibly in the cleft formed by the coiled-coil of the folded dimer
866;Protein component of the large (60S) ribosomal subunit, has similarity to rat L29 ribosomal protein; not essential for translation, but required for proper joining of the large and small ribosomal subunits and for normal translation rate
867;Subunit 6 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1
868;Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
869;One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
870;Endoplasmic reticulum protein that may function as a cochaperone, as suggested by the presence of a DnaJ-like domain
871;Protein required for cell viability
872;Kinetochore protein of unknown function; associated with the essential kinetochore proteins Nnf11p and Spc24p; phosphorylated by both Clb5-Cdk1 and, to a lesser extent, Clb2-Cdk1.
873;Quinolinate phosphoribosyl transferase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway
874;Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36
875;20S proteasome beta-type subunit
876;Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
877;Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p
878;Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p879;Protein required for respiratory growth; null mutation suppresses the Cyc1p translation defect caused by the presence of an aberrant ATG codon upstream of the correct start
880;High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
881;Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations
882;RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
883;DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family
884;Protein that interacts with Rab GTPases; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
885;Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
886;Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
887;Peroxisomal membrane protein that is a central component of the peroxisomal protein import machinery, interacts with PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p
888;Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription
889;Mitochondrial polypeptide chain release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
890;Hypothetical protein
891;N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal
892;Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphophatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
893;Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate
894;Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Grp1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p
895;RNA helicase in the DEAH-box family, involved in release of the lariat-intron from the spliceosome
896;Hypothetical protein
897;Cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C), which is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate
898;Protein kinase activator found in a complex containing Snf1p and members of the Sip1p/Sip2p/Gal83p family; activates the Snf1p protein kinase; involved in expression of glucose-repressed genes, sporulation, and peroxisome biogenesis
899;Putative member of the oligopeptide transporter (OPT) family of membrane transporters
900;Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
【０１３６】
901;Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
902;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
903;Essential light chain for myosin Myo2p; may stabilize Myo2p by binding to the neck region; may interact with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition
904;Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nup120p, Nup145p, and Seh1p); homologous to Sec13p
905;Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
906;Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; potentially phosphorylated by Cdc28p
907;Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
908;Hypothetical protein
909;Plasma membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases
910;Hypothetical protein
911;Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis
912;Transcription factor involved in iron utilization and homeostasis; binds the consensus site PyPuCACCCPu and activates the expression of target genes in response to changes in iron availability
913;RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site
914;Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
915;NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes
916;Essential mitotic spindle protein required to maintain spindle integrity and kinetochore function, part of the multisubunit DASH complex which binds microtubules and is transferred to the kinetochore prior to mitosis
917;Hypothetical protein
918;Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p)
919;One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation
920;Fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
921;Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon
922;Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4631p923;Cleavage and polyadenylation factor I (CF I) component involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p
924;General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites
925;Delta-aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of delta-aminolevulinic acid to porphobilinogen, the second step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus
926;Nicotinamidase that converts nicotinamide to nicotinic acid as part of the NAD(+) salvage pathway, required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span
927;Mtf1 Two Hybrid Clone 2
928;Transcription factor involved in glucose repression; C2H2 zinc finger protein similar to mammalian Egr and Wilms tumor proteins
929;Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
930;Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate
931;Protein involved in nucleolar integrity and processing of the pre-rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress
932;Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
933;Beta regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB2, the many substrates include transcription factors and all RNA polymerases
934;Molecular chaperone involved, with partner Ssq1p, in assembly of Fe/S clusters and in mitochondrial iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
935;Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
936;C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
937;Alpha subunit of the 20S core complex of the 26S proteasome involved in the degradation of ubiquitinated substrates; essential for growth; detected in the mitochondria
938;Hypothetical protein
939;Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway
940;Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium
941;Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
942;Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of mitotic cyclins resulting in exit from mitosis; targets the APC/C to specific substrates including CDC20, ASE1 and CIN8
943;Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
944;C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
945;Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
946;Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p
947;TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
948;Choline phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases
949;Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein
950;t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog
【０１３７】
951;Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway
952;Hypothetical protein
953;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
954;Gamma-aminobutyrate (GABA) transaminase (4-aminobutyrate aminotransferase) involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization
955;Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
956;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
957;Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similarity to rat S25 ribosomal protein
958;Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase
959;Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends
960;Constituent of the mitochondrial inner membrane presequence translocase (TIM23 complex); may regulate protein import by binding to both the translocase of the outer membrane (TOM) and presequence-associated motor (PAM) complexes
961;Protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
962;Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes
963;Evolutionarily conserved protein with similarity to Orm2p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum
964;Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole
965;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
966;Putative protein of unknown function; transcription is repressed by MOT1/YPL082C and induced by alpha-factor and during diauxic shift; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
967;Putative protein of unknown function; mRNA is targeted to the bud via the mRNA transport system involving She2p; deletion mutant is inviable
968;One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
969;Protein that interacts with Cdc48p and Npl4p, involved in recognition of polyubiquitinated proteins and their presentation to the 26S proteasome for degradation; involved in transporting proteins from the ER to the cytosol
970;Hypothetical protein
971;High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
972;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
973;Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI
974;C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
975;Mitochondrial inner membrane protein, required for export of the Cox2p C terminus from the mitochondrial matrix to the intermembrane space during its assembly into cytochrome c oxidase; similar to Oxa2p of N. crassa
976;Putative protein of unknown function
977;GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
978;Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons
979;Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1
980;Unique component of the U4/U6.U5 tri-snRNP particle, dispensable for spliceosome assembly, but required for conformational changes which lead to catalytic activation of the spliceosome
981;Mitochondrial ribosomal protein of the large subunit
982;Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly
983;Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
984;Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
985;Long chain base-responsive inhibitor of protein kinases Pkh1p and Pkh2p, acts along with Lsp1p to down-regulate heat stress resistance via regulation of the Pkc1p and Ypk1p pathways; phosphorylated by Phk1p and Phk2p
986;Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, involved in amino acid catabolism
987;Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis
988;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
989;Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p, which relieves repression of stress-response genes
990;Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
991;Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
992;Hypothetical protein
993;Nucleolar protein involved in rRNA processing and 60S ribosomal subunit biogenesis; constituent of several different pre-ribosomal particles
994;Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
995;Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
996;Hypothetical protein
997;B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome998;Hypothetical protein
999;Mitochondrial inner membrane protein required for normal respiration, possible chaperone involved in assembly of cytochrome c oxidase; similar to SURF1 from mammals, chickens, and D. melanogaster
1000;Component of the DASH complex, localized to intranuclear spindles and spindle pole bodies; interacts with Duo1p and Mps1p; key Ipl1p target for regulating kinetochore-microtubule attachments
【０１３８】
1001;Essential subunit of the nuclear pore complex (NPC), functions as the organizing center of an NPC subcomplex containing Nsp1p, Nup49p, Nup57p, and Nic96p
1002;Hypothetical protein
1003;Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway
1004;Hypothetical protein
1005;Hypothetical protein
1006;Hypothetical protein
1007;Component of the spliceosome complex involved in pre-mRNA splicing; involved in regulation of cell cycle progression
1008;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
1009;Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation
1010;20S proteasome beta-type subunit; the only nonessential 20S subunit
1011;Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
1012;Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p
1013;Essential nucleolar protein of unknown function; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
1014;Hypothetical protein
1015;N alpha-acetyl-transferase, transfers acetyl group from acetyl coenzyme A to the N-terminal methionine residues of proteins
1016;Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate
1017;GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases
1018;Cystathionine beta-synthase, catalyzes the synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis
1019;Pta1p Interacting protein
1020;Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
1021;Putative component of the protein phosphatase type 2A complex
1022;Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p
1023;Pseudouridine synthase responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
1024;Identified by homology to Ashbya gossypii
1025;Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine
1026;Protein with similarity to mammalian developmentally regulated GTP-binding protein
1027;Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
1028;Protein interacting with poly(A)-binding protein Pab1p; likely involved in control of the extent of mRNA polyadenylation; also interacts with Mkt1p to form a complex that may regulate translation of the HO mRNA
1029;Mitochondrial intermembrane space protein, forms a complex with TIm8p that medates import and insertion of a subset of polytopic inner membrane proteins; may prevent aggregation of incoming proteins in a chaperone-like manner
1030;Ubiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2p to ubiquitinate substrates of the N-end rule pathway; binds to the Rpn2p, Rpt1p, and Rpt6p proteins of the 19S particle of the 26S proteasome
1031;Cytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNA synthetase that aminoacylates tRNA(Tyr), required for cytoplasmic protein synthesis, interacts with positions 34 and 35 of the anticodon of tRNATyr
1032;TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
1033;Protein of unknown function with similarity to human HMG1 and HMG2; localizes to the cytoplasm
1034;High-affinity histidine permease, also involved in the transport of manganese ions
1035;Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall 
1036;Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
1037;3'-to-5' phosphorolytic exoribonuclease that is a subunit of the exosome; required for 3' processing of the 5.8S rRNA; involved in 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs
1038;Protein of unknown function; homolog of mammalian electron transfer flavoprotein complex subunit ETF-beta; interacts with frataxin (Yfh1p)
1039;Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
1040;N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
1041;Mitochondrial ribosomal protein of the small subunit
1042;Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
1043;Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus
1044;Dolichyl-phosphoglucose-dependent glucosyltransferase of the ER, functions in the dolichol pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1
1045;Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
1046;Regulatory, non-ATPase subunit of the 26S proteasome; homolog of the human oncoprotein gankyrin, which interacts with the retinoblastoma tumor suppressor (Rb) and cyclin-dependent kinase 4/6
1047;Putative protein of unknown function; localizes to the mitochondrion
1048;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1049;The authentic, non-tagged protein was localized to mitochondria
1050;Cyclic nucleotide phosphodiesterase, hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; no detectable phenotype is conferred by null mutation or by overexpression
【０１３９】
1051;6-phosphogluconolactonase with similarity to Sol3p
1052;Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
1053;Histone acetyltransferase, acetylates lysine 14 on histone H3; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; founding member of the Gcn5p-related N-acetyltransferase superfamily
1054;Alpha subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
1055;Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose
1056;Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
1057;High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA)
1058;Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
1059;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
1060;GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
1061;Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
1062;TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation, has histone acetyltransferase activity, involved in promoter binding and G1/S progression
1063;3' exoribonuclease, required for 5S and tRNA-Arg3 maturation
1064;Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating
1065;Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
1066;Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance
1067;Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo
1068;Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
1069;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1070;Nucleolar single-strand nucleic acid binding protein; associates with small nuclear RNAs
1071;Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits
1072;Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
1073;v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28
1074;Major component of the proteasome; tethers the proteasome core particle to the regulatory particle, and enhances the stability of the proteasome
1075;Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
1076;Transcriptional repressor involved in the response to pH; required for alkaline pH-stimulated differentiation pathways such as haploid invasive growth and sporulation; activated by proteolytic processing; has similarity to the A. nidulans transcription factor PacC
1077;One of 11 subunits of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p
1078;Putative RNA-binding protein required for the expression of early and middle sporulation genes
1079;Protein required for sporulation
1080;The authentic, non-tagged protein was localized to the mitochondria
1081;Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
1082;Plasma membrane urea transporter, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
1083;Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; has similarity to E. coli S10 and rat S20 ribosomal proteins
1084;Putative protein of unknown function; predicted to be a member of the ovarian tumor-like (OTU) superfamily of cysteine proteases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1085;Putative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase
1086;5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a five related enzymes, which are active as heteromultimeric complexes
1087;Putative protein of unknown function, contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences
1088;Signal transducing kinase of the PAK (p21-activated kinase) family, involved in pheromone response and pseudohyphal/invasive growth pathways, activated by Cdc42p; binds Ste4p at a GBB motif present in noncatalytic domains of PAK kinases
1089;Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p
1090;Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
1091;Mitochondrial carrier protein involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein; does not encode an isozyme of Leu4p, as first hypothesized
1092;Putative protein of unknown function, localizes to the mitochondria
1093;Protein of the nuclear envelope required for the spherical shape of the nucleus; required for normal sporulation
1094;GTP-binding alpha subunit of the heterotrimeric G protein that couples to pheromone receptors; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates the pathway via Scp160p
1095;Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that mediates insertion of hydrophobic proteins at the inner membrane, has homology to Mrs5p, which is also involved in this process1096;Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes
1097;Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family
1098;Manganese-containing superoxide dismutase; protects cells against oxygen toxicity
1099;Putative protein of unknown function; not an essential gene
1100;Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth
【０１４０】
1101;Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
1102;Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II
1103;Putative protein of unknown function, has similarity to proline-tRNA ligase; YHR020W is an essential gene
1104;Larger subunit of the mitochondrial processing protease, essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
1105;Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
1106;Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions1107;Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
1108;Protein of unknown function that is a member of the PhzF superfamily, although unlike its bacterial homolog, is most likely not involved in phenazine production; possibly involved in a membrane regulation metabolic pathway
1109;Protein of unknown function that is a member of the PhzF superfamily, although unlike its bacterial homolog, is most likely not involved in phenazine production; possibly involved in a membrane regulation metabolic pathway
1110;Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progression through the cell cycle; regulated by the PKC1-mediated signaling pathway
1111;DNA helicase involved in rDNA replication and Ty1 transposition; structurally and functionally related to Pif1p
1112;Protein of unresolved function; may function in protein folding and/or rRNA processing, interacts with a chaperone (Hsp82p), two chromatin remodeling factors (Rvb1p, Rvb2p) and two rRNA processing factors (Rrp43p, Nop58p)
1113;Essential nuclear envelope integral membrane protein identified as a suppressor of a cold-sensitive mutant of CRM1, a karyopherin; homologous to and interacts with Brr6p, which is a nuclear envelope protein involved in nuclear export
1114;Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria
1115;Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids
1116;NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
1117;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
1118;Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
1119;Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation
1120;Serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to Fsh2p and Fsh3p
1121;Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
1122;Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
1123;Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
1124;Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
1125;Protein of unknown function, required for survival upon exposure to K1 killer toxin
1126;Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family
1127;Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
1128;Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
1129;Protein involved in rRNA processing; component of the exosome 3->5 exonucle
ase complex with Rrp41p, Rrp42p, Rrp43p and Dis3p
1130;tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya
1131;Constituent of small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs, which are required for pseudouridylation and processing of pre-18S rRNA
1132;Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
1133;Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
1134;Serine-threonine kinase and endoribonuclease; transmembrane protein that initiates the unfolded protein response signal by regulating synthesis of Hac1p through HAC1 mRNA splicing
1135;Nucleolar protein involved in the assembly of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
1136;Subunit of the NuA4 histone acetyltransferase complex that acetylates histone H4 and H2A; has similarity to the human tumor suppressor ING1
1137;Mitochondrial arginyl-tRNA synthetase
1138;Member of the Sec24p family; forms a complex, with Sec23p, that is involved in sorting of Pma1p into COPII vesicles; peripheral ER membrane protein; potential Cdc28p substrate
1139;Hypothetical protein
1140;Protein kinase of the PAK/Ste20 kinase family, required for cell integrity possibly through regulating 1,6-beta-glucan levels in the wall; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body
1141;Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
1142;Aldose reductase involved in methylglyoxal, d-xylose and arabinose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway
1143;Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi
1144;Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
1145;Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response
1146;Hypothetical protein
1147;Cytoplasmic aspartyl aminopeptidase; cleaves unblocked N-terminal acidic amino acid residues from peptide substrates; forms a 12 subunit homo-oligomeric complex; inhibited by EDTA and 1,10-phenanthroline; M18 metalloprotease family member
1148;Protein involved in regulating spindle position and orientation, functionally redundant with Dma2p; homolog of S. pombe Dma1 and H. sapiens Chfr
1149;Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins
1150;Histone methyltransferase, subunit of the COMPASS (Set1C) complex which methylates histone H3 on lysine 4; required in transcriptional silencing near telomeres and at the silent mating type loci; contains a SET domain
【０１４１】
1151;DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
1152;Protein containing an Lsm domain and an AD domain; may bind RNA and have a role in RNA processing
1153;Protein required for cell viability
1154;Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE)
1155;(H)igh copy (S)uppressor of (N)34 dominant negative allele of SEC4. Suppression is very specific to this allele. It has no affect on the analogous YPT1 allele. No homology or known function.
1156;Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
1157;Hypothetical protein
1158;Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p
1159;Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall
1160;Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p export from the ER
1161;RNA polymerase subunit, found in RNA polymerase complexes I, II, and III
1162;Deaminase required for dCTP and dTTP synthesis; expression is cell cycle regulated
1163;Mitochondrial ribosomal protein of the large subunit
1164;Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
1165;Hypothetical protein
1166;Nucleolar protein of unknown function, positive regulator of exit from mitosis; involved in regulating the release of Cdc14p from the nucleolus in early anaphase; proposed to play similar role in meiosis
1167;Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
1168;Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
1169;Part of a two-member peroxin family (Pex18p and Pex21p) specifically required for peroxisomal targeting of the Pex7p peroxisomal signal recognition factor and PTS2 peroxisomal matrix proteins
1170;Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1802p, member of the AP180 protein family
1171;Hypothetical protein
1172;Essential tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair pathways; potential Cdc28p substrate
1173;Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing
1174;Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
1175;Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
1176;Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
1177;Activator of multidrug resistance genes, forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a PDRE (pleotropic drug resistance element) in vitro; binds Sin3p in a two-hybrid assay
1178;Integral membrane protein of the early Golgi apparatus, may function to promote retention of proteins in the early Golgi compartment; mutation affects protein N-glycosylation and cell wall integrity
1179;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm
1180;6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress
1181;Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis
1182;Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
1183;Subunit of the Elp4p-Iki1p-Elp6p-subcomplex of RNA polymerase II elongator complex, which is a histone acetyltransferase; iki1 mutations confer resistance to the K. lactis toxin zymocin
1184;Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
1185;One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for cell growth and for mitochondrial respiration
1186;Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
1187;Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion
1188;Mitochondrial inner membrane protein with similarity to Mdm32p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
1189;Nuclear envelope protein that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
1190;Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
1191;The authentic, non-tagged protein was localized to the mitochondria
1192;Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein
1193;Hypothetical protein
1194;Alpha mannosidase-like protein of the endoplasmic reticulum required for degradation of glycoproteins but not for processing of N-linked oligosaccharides
1195;Protein kinase that regulates signal transduction activity and G1 progression, controls cAPK activity, required for nitrogen activation of the FGM pathway, involved in life span regulation, homologous to mammalian Akt/PKB
1196;Nuclear response regulator and transcription factor, part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation
1197;Zinc-finger protein of unknown function, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
1198;Mitochondrial branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase
1199;Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family
1200;Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
【０１４２】
1201;3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
1202;Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1
1203;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
1204;Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
1205;Hypothetical protein
1206;Hypothetical protein
1207;Essential, chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
1208;Non-essential protein of unknown function
1209;Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3
1210;Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
1211;Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
1212;Minor isoform of tropomyosin, binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p
1213;Putative metalloprotease
1214;Protein of unknown function, major constituent of the mitochondrial outer membrane; located on the outer (cytosolic) face of the outer membrane
1215;Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
1216;Protein required for transport of flavin adenine dinucleotide (FAD) from mitochondria, where it is synthesized from riboflavin, to the cytosol
1217;Proposed transcriptional activator, member of the Gal4p family of zinc cluster proteins
1218;DNA repair and TFIIH regulator, required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription; possible role in assembly of a multiprotein complex(es) required for NER and RNAP II transcription
1219;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
1220;Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA
1221;NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles and ER; involved in phosphatidic acid biosynthesis and required for spore germination; capable of metabolizing mammalian steroid hormones
1222;Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
1223;Putative transcriptional activator that promotes recovery from pheromone induced arrest; inhibits both alpha-factor induced G1 arrest and repression of CLN1 and CLN2 via SCB/MCB promoter elements; potential Cdc28p substrate; SBF regulated
1224;Putative transcriptional regulator; overexpression suppresses the heat shock sensitivity of wild-type RAS2 overexpression and also suppresses the cell lysis defect of an mpk1 mutation
1225;Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
1226;Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock
1227;Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate
1228;Component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat; involved in ER to Golgi transport, cargo selection and autophagy; required for the binding of the Sec13 complex to ER membranes; homologous to Lst1p and Lss1p
1229;6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, has negligible fructose-2,6-bisphosphatase activity, transcriptional regulation involves protein kinase A
1230;Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; subunit of and phosphorylated by the TORC2 complex
1231;Essential nuclear protein, required for accumulation of box H/ACA snoRNAs and for rRNA processing; interacts with Naf1p
1232;Hypothetical protein
1233;Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton through the phosphorylation of the Pan1p-Sla1p-End3p protein complex
1234;Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate
1235;Mitochondrial ribosomal protein of the small subunit
1236;Protein required for cell viability
1237;Integral ER membrane protein with type-III transmembrane domains; mutations cause defects in cortical ER morphology in both the mother and daughter cells
1238;Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
1239;Hypothetical protein
1240;Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
1241;Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes
1242;Homolog to human PPCS
1243;RING finger protein that interacts with the arginine methyltransferase Hmt1p to regulate methylation of Npl3p, which modulates Npl3p function in mRNA processing and export; has similarity to Air2p
1244;Threonyl-tRNA synthetase, essential cytoplasmic protein
1245;Subunit of the 26S proteasome, substrate of the N-acetyltransferase Nat1p
1246;3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser3p
1247;Meiosis-specific DNA binding protein that displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for homologous chromosome synapsis and chiasma formation
1248;Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R
1249;Hypothetical protein
1250;Mitochondrial outer membrane protein involved in membrane fission, required for localization of Dnm1p and Mdv1p during mitochondrial division
【０１４３】
1251;Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
1252;Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
1253;Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein
1254;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
1255;Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and, along with the Hor2p/Gpp2p isoform, osmotic stress
1256;Mitochondrial protein involved in maintenance of the mitochondrial genome
1257;Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated
1258;Protein of unknown function, involved in filamentous growth
1259;Protein involved in the retrograde transport from the Golgi complex to the ER and the endosomal membrane traffic; putative aminophospholipid translocase; member of the highly conserved Drs2 family of P-type ATPases
1260;Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency
1261;F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
1262;Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase
1263;ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
1264;Hypothetical protein
1265;Golgi vesicle protein of unknown function; localizes to both early and late Golgi vesicles
1266;Protein involved in nucleocytoplasmic transport of mRNA
1267;Hypothetical protein
1268;Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
1269;Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p
1270;Alpha catalytic subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA2, CKB1 and CKB2, the many substrates include transcription factors and all RNA polymerases
1271;Beta subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches
1272;Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation1273;Protein of unknown function; null mutant shows K1 killer toxin resistance
1274;Hypothetical protein
1275;Yeast KE4, yeast ortholog of the mouse KE4
1276;Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the translocon channel at the matrix side of the inner membrane
1277;RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
1278;Protein required for pre-rRNA processing and 40S ribosomal subunit assembly1279;Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm
1280;Protein of unknown function proposed to be involved in nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein
1281;Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
1282;Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth
1283;Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
1284;Ubiquitin-like protein with only weak sequence similarity to ubiquitin; depends on the E1-like activating enzyme Uba4p; molecular function of the Urm1p pathway is unknown, but it is required for normal growth, particularly at high temperature
1285;Protein with similarity to the p27 subunit of mammalian proteasome modulator; not essential; interacts with Rpn4p
1286;Pdi1p (protein disulfide isomerase)-related protein involved in endoplasmic reticulum retention of resident ER proteins
1287;Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
1288;Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype
1289;Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ
1290;Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; previously thought to be a subunit of poly(A) ribonuclease
1291;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene
1292;Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
1293;U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro; does not contain the conserved C-terminal RNA binding domain found in other family members
1294;Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
1295;Protein containing GATA family zinc finger motifs
1296;18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
1297;Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
1298;Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
1299;Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
1300;Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression
【０１４４】
1301;ER membrane protein involved, with its homolog Spt23p, in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
1302;Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway
1303;Hypothetical protein
1304;Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor
1305;Putative GPI-anchored aspartic protease
1306;Putative protein of unknown function; YIR042C is a non-essential gene
1307;Putative protein of unknown function; YIR042C is a non-essential gene
1308;Hypothetical protein
1309;Plasma membrane transporter that transports tetra- and pentapeptides and glutathione; member of the OPT family
1310;RING-finger peroxin, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms putative translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import
1311;Mitochondrial protein that interacts with the 5'-untranslated region of the COB mRNA and has a role in its stability and translation; found in a complex at the inner membrane along with Pet309p
1312;Hypothetical protein
1313;F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
1314;Subunit of the SF3a splicing factor complex, required for spliceosome assembly
1315;Hypothetical protein
1316;Ubiquitin-specific protease present in the nucleus and cytoplasm that cleaves ubiquitin from ubiquitinated proteins
1317;Hypothetical protein
1318;Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate
1319;Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
1320;Hypothetical protein
1321;Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
1322;Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase
1323;Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin
1324;One of 11 subunits of the SWI/SNF chromatin remodeling complex that regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2
1325;Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
1326;Subunit of heterotrimeric Replication Factor A (RF-A), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
1327;Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions
1328;Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
1329;Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
1330;Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters
1331;Subunit of cytoplasmic cAMP-dependent protein kinase, which contains redundant catalytic subunits Tpk1p, Tpk2p, and Tpk3p and regulatory subunit Bcy1p; promotes vegetative growth in response to nutrients; inhibits filamentous growth
1332;The authentic, non-tagged protein was localized to the mitochondria
1333;Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
1334;Fructose-2,6-bisphosphatase, required for glucose metabolism
1335;Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps26p and Vps29p that selects cargo proteins for endosome-to-Golgi retrieval1336;Hypothetical protein; has similarity to F-box proteins
1337;RNA polymerase I subunit A34.5
1338;Hypothetical protein
1339;Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
1340;Hypothetical protein
1341;Essential constituent of the mitochondrial inner membrane presequence translocase; interacts with Pam18p to recruit the presequence translocase-associated motor (PAM complex) and also required for protein sorting during import
1342;Serine-threonine protein kinase that is part of a glucose-sensing system involved in growth control in response to glucose availability; translocates from the cytoplasm to the nucleus and phosphorylates Pop2p in response to a glucose signal
1343;RNA polymerase II subunit B32; forms two subunit dissociable complex with Rpb7p; dispensable under some environmental conditions; involved in export of mRNA to cytoplasm under stress conditions
1344;Translation initiation factor eIF4A, identical to Tif1p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G1345;Long-chain base-1-phosphate phosphatase, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids
1346;ORF, Uncharacterized
1347;Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrs4p; functions under low-iron conditions; may transport other cations in addition to iron
1348;MAP kinase kinase that plays a pivotal role in the osmosensing signal-transduction pathway, activated under severe osmotic stress
1349;Identified by homology to Ashbya gossypii
1350;Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
【０１４５】
1351;Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs
1352;Hypothetical protein
1353;Endoplasmic reticulum (ER) resident protein required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles
1354;Protein that functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p ) of the Fo-F1 ATP synthase; member of the SUN family
1355;Nucleosome assembly factor, involved in chromatin assembly after DNA replication, anti-silencing protein that causes derepression of silent loci when overexpressed
1356;Peripheral protein of the cytosolic face of the mitochondrial outer membrane, required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats
1357;Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p
1358;Constituent of the mitochondrial import motor associated with the presequence translocase, along with Ssc1p, Tim44p, Mge1p, and Pam18p; has similarity to J-domain containing proteins
1359;Hypothetical protein
1360;Phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
1361;Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p
1362;DNA helicase and DNA-dependent ATPase involved in DNA repair, required for proper timing of commitment to meiotic recombination and the transition from Meiosis I to Meiosis II; potential Cdc28p substrate
1363;Essential BRCT repeat protein, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M checkpoint control
1364;C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
1365;Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
1366;tRNA ligase, required for tRNA splicing; composed of three essential domains containing the phosphodiesterase, polynucleotide kinase, and ligase activities required for ligation; localized at the inner membrane of the nuclear envelope
1367;Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis
1368;Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
1369;Essential RNA-binding G protein effector of mating response pathway, predominantly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
1370;Protein of unknown function, has similarity to Pry2p and Pry3p and to the plant PR-1 class of pathogen related proteins
1371;Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
1372;Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
1373;Hypothetical protein
1374;Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
1375;Subunit of ISW2/yCHRAC chromatin accessibility complex along with Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing
1376;Mitochondrial ribosomal protein of the large subunit
1377;Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity
1378;Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup159p and Nsp1p, interacts with Nup116p and is required for proper localization of Nup116p in the NPC
1379;Arylformamidase, involved in biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; potential Cdc28p substrate
1380;Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis)
1381;Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p-Slm1p-Slm2p), a membrane-associated complex that regulates cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity
1382;probable serine/threonine kinase
1383;Vacuolar protein sorting protein that forms part of the multimeric membrane-associated retromer complex along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport
1384;Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall 
1385;Dead-box family ATP dependent helicase required for mRNA export from the nucleus; co-factor of the exosome complex, required for 3' end formation of 5.8S rRNA
1386;Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner
1387;Putative protein of unknown function; expression induced during sporulation and repressed during vegetative growth by Sum1p and Hst1p; similar to adjacent open reading frame, YJL037W
1388;Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX domain; forms complex with Snx41p and Atg20p
1389;Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
1390;ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
1391;Component of the spindle-assembly checkpoint complex, which delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p
1392;Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
1393;Essential integral membrane protein required for spindle pole body duplication and for nuclear fusion, localizes to the spindle pole body half bridge, interacts with DnaJ-like chaperone Jem1p and with centrin homolog Cdc31p
1394;RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex
1395;Essential nucleolar protein required for 18S rRNA synthesis
1396;Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
1397;Hypothetical protein
1398;Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing; has similarity to cyclins
1399;Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; involved in cell cycle control and glucose and nitrogen repression of sporulation
1400;Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
【０１４６】
1401;Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
1402;Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase
1403;Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase
1404;Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
1405;Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
1406;Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
1407;Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
1408;Hypothetical protein
1409;Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor
1410;Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
1411;Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
1412;Identified by homology to Ashbya gossypii
1413;Identified by homology to Ashbya gossypii
1414;DNA polymerase III (delta) subunit, essential for cell viability; involved in DNA replication and DNA repair
1415;DNA polymerase III (delta) subunit, essential for cell viability; involved in DNA replication and DNA repair
1416;Alpha subunit of the translation initiation factor eIF2, involved in the identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP
1417;Alpha subunit of the translation initiation factor eIF2, involved in the identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP
1418;Hypothetical protein
1419;Subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER), homolog of the SPC12 subunit of mammalian signal peptidase complex
1420;Hypothetical protein
1421;Hypothetical protein
1422;Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
1423;Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
1424;3-hydroxyanthranilic acid dioxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway
1425;Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity
1426;Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
1427;Protein required for assembly of cytochrome c oxidase
1428;Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability
1429;Chloride channel localized to late- or post-Golgi vesicles, involved in iron metabolism; highly homologous to voltage-gated chloride channels in vertebrates
1430;Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis
1431;Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
1432;Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP)
1433;Mitochondrial matrix ATPase that is a subunit of the presequence translocase-associated protein import motor (PAM); involved in protein translocation into the matrix and protein folding; member of the heat shock protein 70 (HSP70) family
1434;Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Hyp2p; undergoes an essential hypusination modification; expressed under anaerobic conditions
1435;Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration
1436;NAD kinase, active as a hexamer; enhances the activity of ferric reductase (Fre1p)
1437;Component of the spliceosome complex involved in pre-mRNA splicing, auxiliary splicing factor that may modulate Syf1p activity and help optimize splicing; isy1 syf2 double mutation activates the spindle checkpoint, causing cell cycle arrest
1438;Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity
1439;Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4)
1440;Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis
1441;Vacuolar protein of unknown function; potential Cdc28p substrate
1442;Protein of unknown function, required for growth at high temperature
1443;Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
1444;Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
1445;RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
1446;Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
1447;Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
1448;PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis
1449;Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
1450;Protein of unknown function that is involved in DNA repair; mutant is sensitive to the base analog, 6-N-hydroxylaminopurine, while gene disruption does not increase the rate of spontaneous mutagenesis
【０１４７】
1451;Cytoplasmic protein required for cell viability, identified by association with pre-ribosomal particles
1452;Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
1453;Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM
1454;Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2 under normal conditions
1455;The authentic, non-tagged protein was localized to the mitochondria
1456;Esa1p-associated factor, subunit of the NuA4 acetyltransferase complex
1457;Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
1458;Putative protein of unknown function; detectable in highly purified mitochondria
1459;G protein gamma subunit, forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling
1460;Essential chromosomal passenger protein involved in coordinating cell cycle events for proper chromosome segregation; C-terminal region binds Sli15p, and the middle region, upon phosphorylation, localizes Cbf2p to the spindle at anaphase
1461;Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells
1462;Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity
1463;Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
1464;Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization1465;Putative xylose and arabinose reductase
1466;Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
1467;Mitochondrial ribosomal protein of the small subunit
1468;Cu, Zn superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans
1469;Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
1470;Phosphatidylinositol 3-phosphate [PI(3)P] phosphatase, regulates the localization and levels of PI(3)P; involved in cytoplasm to vacuole (CVT) transport; has similarity to the conserved myotubularin dual specificity phosphatase family
1471;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
1472;Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
1473;Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein
1474;Hypothetical protein
1475;Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
1476;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
1477;Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
1478;Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; has similarity to E. coli S7 and rat S5 ribosomal proteins
1479;Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p
1480;Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1481;Cystathionine gamma-synthase, converts cysteine into cystathionine
1482;Karyopherin, a carrier protein involved in nuclear import of proteins; importin beta homolog
1483;Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine
1484;Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus
1485;Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner
1486;Mitochondrial intermembrane space protein mediating import and insertion of polytopic inner membrane proteins; homolog of human DDP1 (deafness dystonia peptide 1) which is mutated in the X-linked Mohr-Tranebjaerg syndrome
1487;Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
1488;Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane
1489;Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
1490;Transcriptional corepressor involved in the cell cycle-regulated transcription of histone genes HTA1, HTB1, HHT1, and HHT2; involved in position-dependent gene silencing and nucleosome reassembly
1491;Putative protein of unknown function
1492;Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals
1493;Cytosolic branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase
1494;Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
1495;Allantoin permease; ureidosuccinate permease; expression is constitutive but sensitive to nitrogen catabolite repression
1496;Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins
1497;Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins
1498;Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
1499;Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
1500;WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase
【０１４８】
1501;Lipid phosphoinositide phosphatase of the ER and Golgi, involved in protein trafficking and secretion
1502;Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p1503;Ubiquitin activating enzyme, involved in ubiquitin-mediated protein degradation and essential for viability
1504;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1505;Nuclear pore protein involved in nuclear export of pre-tRNA
1506;PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis
1507;Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
1508;Mitochondrial threonyl-tRNA synthetase
1509;Conserved nuclear regulatory subunit of Glc7p type 1 protein serine-threonine phosphatase (PP1), functions positively with Glc7p to promote dephosphorylation of nuclear substrates required for chromosome transmission during mitosis
1510;Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
1511;Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
1512;Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response
1513;Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines1514;5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
1515;Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles
1516;Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels
1517;Subunit of cytoplasmic cAMP-dependent protein kinase, which contains redundant catalytic subunits Tpk1p, Tpk2p, and Tpk3p and regulatory subunit Bcy1p; promotes vegetative growth in response to nutrients; inhibits filamentous growth
1518;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
1519;Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
1520;Merged open reading frame, does not encode a discrete protein; YKL158W was originally annotated as an independent ORF, but was later demonstrated to be an exon of an adjacent ORF, YKL157W
1521;Mitochondrial ribosomal protein of the small subunit
1522;Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
1523;Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis
1524;RNA polymerase III subunit C25
1525;Putative mitochondrial ribosomal protein, has similarity to E. coli ribosomal protein S2
1526;Cytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone
1527;Component of the DASH complex which is found at kinetochores and regulates spindle assembly; in vitro DASH forms rings on microtubules and promotes microtubule stability
1528;Mitochondrial ribosomal protein of the large subunit
1529;Putative dihydrofolate synthetase; has similarity to Fol3p; similar to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase
1530;RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud
1531;Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA
1532;Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
1533;Putative protein of unknown function
1534;Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, and thiosulfate; member of the mitochondrial carrier family
1535;Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER)
1536;Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins
1537;Protein kinase with a possible role in MAP kinase signaling in the pheromone response pathway
1538;5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family
1539;Protein associated with the RNA polymerase II Elongator complex; involved in sensitivity to G1 arrest induced by Kluyveromyces lactis toxin, zymocin
1540;Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
1541;Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
1542;Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis
1543;Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
1544;Cell wall mannoprotein, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; involved in cell wall organization
1545;Protein of unknown function, localizes to lipid particles
1546;Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants
1547;Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination
1548;Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p
1549;Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
1550;Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle
【０１４９】
1551;Mitochondrial intermembrane space protein, first component of a pathway mediating assembly of small TIM (Translocase of the Inner Membrane) complexes which escort hydrophobic inner membrane proteins en route to the TIM22 complex
1552;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1553;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
1554;Nucleoside diphosphate kinase, catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate
1555;Endoplasmic reticulum transmembrane protein, homolog of human BAP31 protein1556;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
1557;Transcriptional activator related to Msn2p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression
1558;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endosome
1559;Fructose 1,6-bisphosphate aldolase, a cytosolic enzyme required for glycolysis and gluconeogenesis; catalyzes the conversion of fructose 1,6 bisphosphate into two 3-carbon products: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate
1560;TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA
1561;Protein of unknown function; putative ribosomal protein; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YKL056C is not an essential gene
1562;Protein of unknown function; mutation affects mitochondrial distribution and morphology
1563;Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p
1564;Transcriptional activator that enhances pseudohyphal growth; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate
1565;Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
1566;One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Vps2p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
1567;Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor
1568;UDP-glucose pyrophosphorylase (UGPase), catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p
1569;Golgi-localized RING-finger ubiquitin ligase (E3), involved in ubiquitinating and sorting membrane proteins that contain polar transmembrane domains to multivesicular bodies for delivery to the vacuole for quality control purposes
1570;Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b
1571;Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
1572;TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
1573;Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
1574;Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
1575;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1576;ER membrane protein involved, with its homolog Mga2p, in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
1577;Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
1578;Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1579;Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
1580;Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
1581;Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
1582;Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences and undergoes a conformational change to form the active state; has a Zn(2)-Cys(6) binuclear cluster domain
1583;Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit
1584;Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
1585;Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
1586;Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins
1587;Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus
1588;Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p
1589;Alpha subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches
1590;Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit
1591;Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
1592;Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator
1593;Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism
1594;GTPase required for vacuolar protein sorting, functions in actin cytoskeleton organization via its interaction with Sla1p; required for late Golgi-retention of some proteins including Kex2p; involved in regulating peroxisome biogenesis
1595;Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs
1596;Member of an oxysterol-binding protein family with overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery
1597;Non-essential protein of unknown function
1598;Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation
1599;Component of the EGO complex, which is involved in the regulation of microautophagy; localizes to the vacuolar membrane, loss results in a defect in vacuolar acidification
1600;One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling
【０１５０】
1601;Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
1602;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
1603;Protein of unknown function, has similarity to Pry1p and Pry3p and to the plant PR-1 class of pathogen related proteins
1604;Putative protein of unknown function; contains a RING finger motif
1605;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
1606;Protein involved in regulation of phosphatidylinositol 4,5-bisphosphate concentrations; Irs4p and Tax4p bind and activate the phosphatase Inp51p; mutation confers an increase in rDNA silencing
1607;Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP)  complex to the SNARE Tlg1p
1608;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and mitochondrion
1609;Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis
1610;Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
1611;The authentic, non-tagged protein was localized to the mitochondria
1612;Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains
1613;Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting
1614;Class E protein of the vacuolar protein-sorting (Vps) pathway, associates reversibly with the late endosome, has human ortholog that may be altered in breast tumors
1615;General amino acid permease; localization to the plasma membrane is regulated by nitrogen source
1616;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
1617;Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates
1618;Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange
1619;Mitochondrial protein of unknown function; putative redox protein containing a thioredoxin fold
1620;Hypothetical protein
1621;Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrs3p; functions under low-iron conditions; may transport other cations in addition to iron
1622;Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity
1623;Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G1624;Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
1625;Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
1626;TFIIE small subunit, involved in RNA polymerase II transcription initiation1627;Essential nuclear protein possibly involved in bud formation and morphogenesis; mutants require the SSD1-v allele for viability
1628;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; detectable in highly purified mitochondria
1629;Presequence translocase-associated motor subunit, required for stable complex formation between cochaperones Pam16p and Pam18p, promotes association of Pam16p-Pam18p with the presequence translocase
1630;Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress
1631;Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
1632;Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
1633;Protein of unknown function; mutation displays synthetic lethal interaction with the pol3-13 allele of CDC2
1634;Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
1635;Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p
1636;Non-essential protein of unknown function; similar to Ygr154cp
1637;Hypothetical protein
1638;tRNase Z, involved in RNA processing, has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif, homolog of the human candidate prostate cancer susceptibility gene ELAC2
1639;NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
1640;Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
1641;Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p
1642;Essential protein, component of the DASH complex; involved in spindle integrity and kinetochore function; interacts with Duo1p and Dam1p; localizes to intranuclear spindles and kinetochore
1643;GTP binding protein with sequence similarity to the elongation factor class of G proteins, EF-1alpha and Sup35p; associates with Dom34p, and shares a similar genetic relationship with genes that encode ribosomal protein components
1644;Mitochondrial ribosomal protein of the large subunit
1645;RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity
1646;Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
1647;Triacylglycerol lipase involved in TAG mobilization; found in lipid particles; potential Cdc28p substrate
1648;Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
1649;Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
1650;Hypothetical protein
【０１５１】
1651;Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins
1652;Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways
1653;S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
1654;High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
1655;Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
1656;Hypothetical protein
1657;Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
1658;Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mammalian bile transporters
1659;Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits
1660;Plasma membrane glycerol channel, member of the major intrinsic protein (MIP) family of channel proteins; involved in efflux of glycerol and in uptake of the trivalent metalloids arsenite and antimonite
1661;Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone
1662;Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention
1663;Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response
1664;Protein of unknown function, contains an N-terminal epsin-like domain
1665;Protein of unknown function, required for cell growth and possibly involved in rRNA processing; mRNA is cell cycle regulated
1666;Hypothetical protein
1667;ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
1668;Hypothetical protein
1669;Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane
1670;Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation
1671;Hypothetical protein
1672;Cytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
1673;Hypothetical protein
1674;Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p
1675;Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase
1676;Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
1677;Hypothetical protein
1678;Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
1679;Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis
1680;Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation
1681;Hypothetical protein
1682;Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p1683;Essential nuclear protein required for DNA repair; forms a complex with Smc5p and Smc6p
1684;Ribosomal Like Protein 24
1685;Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
1686;Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
1687;Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression
1688;Integral membrane protein of the nuclear pore complex, localizes adjacent to the nuclear membrane
1689;Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p
1690;Hypothetical protein
1691;Essential protein involved in RNA metabolism, one of two yeast homologs (with Yhr087wp) of the human protein SBDS responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome, also conserved in Archaea
1692;One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
1693;cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
1694;Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine
1695;Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA
1696;Single-stranded DNA-dependent ATPase, involved in postreplication repair; contains RING finger domain
1697;Remodels the structure of chromatin complex 58KDa subunit; Chromatin Remodeling Complex subunit
1698;Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins
1699;Subunit VIb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of fully active cytochrome c oxidase but not required for activity after assembly
1700;Hypothetical protein
【０１５２】
1701;Hypothetical protein
1702;Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against both oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance
1703;Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
1704;Hypothetical protein
1705;Protein required for cell viability
1706;Subunit of the INO80 chromatin remodeling complex
1707;Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
1708;C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources
1709;Hypothetical protein
1710;Cytosolic serine hydroxymethyltransferase, involved in one-carbon metabolism
1711;RNA exonuclease, required for U4 snRNA maturation; functions redundantly with Rnh70p in 5.8S rRNA maturation, and with Rnh70p and Rex3p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases
1712;Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate the active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
1713;Hypothetical protein
1714;Hypothetical protein
1715;Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
1716;Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; located in the mitochondrial inner membrane
1717;Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in processing the 35S rRNA primary transcript to generate the 20S and 27SA2 pre-rRNA transcripts
1718;Xylitol dehydrogenase, converts xylitol to D-xylulose in the endogenous xylose utilization pathway
1719;Component of RNA polymerase II holoenzyme/mediator complex; affects chromatin structure and transcriptional regulation of diverse genes; required for glucose repression, HO repression, RME1 repression and sporulation
1720;Hypothetical protein
1721;Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family
1722;The authentic, non-tagged protein was localized to the mitochondria
1723;N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p
1724;Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
1725;Putative chaperone, homolog of E. coli DnaJ, closely related to Ydj1p
1726;High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
1727;Protein of unknown function
1728;DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
1729;Protein of unknown function, null mutation leads to an increase in sensitivity to Calcofluor white; expression of the gene is induced in the presence of isooctane
1730;Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36.
1731;3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
1732;Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
1733;DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes1734;Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
1735;Hypothetical protein
1736;Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
1737;Aspartic protease, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
1738;Hypothetical protein
1739;Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p
1740;Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
1741;Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor
1742;Choline kinase, catalyzes the first step in the CDP-choline pathway phosphatidylcholine synthesis (Kennedy pathway); mRNA expression is regulated by inositol and choline, enzyme activity is stimulated by phosphorylation by protein kinase
1743;Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism
1744;mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis
1745;Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH
1746;Mitochondrial membrane protein required for assembly of respiratory-chain enzyme complexes III and IV; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
1747;Hypothetical protein
1748;Protein required for cell viability, component of RNase MRP, which is involved in RNA processing in mitochondria
1749;Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
1750;Vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis, forms complex with Pep5p that mediates protein transport to the vacuole
【０１５３】
1751;Hypothetical protein
1752;Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations
1753;Hypothetical protein
1754;Ribonuclease H2 subunit, required for RNase H2 activity
1755;Smaller subunit of the mitochondrial processing protease, essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
1756;Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B
1757;possibly involved in intramitochondrial sorting
1758;Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
1759;Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
1760;Component of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), probable rRNA pseudouridine synthase, binds to snoRNP Nop10p and also interacts with ribosomal biogenesis protein Nop53p
1761;Hypothetical protein
1762;Carboxypeptidase Y inhibitor, function requires acetylation by the NatB N-terminal acetyltransferase; phosphatidylethanolamine-binding protein involved in protein kinase A signaling pathway
1763;Protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
1764;S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)
1765;Transcription factor that binds to a number of promoter regions, particularly promoters of some genes involved in pheromone response and cell cycle; potential Cdc28p substrate; expression is induced in G1 by bound SBF
1766;Protein required for the maturation of the 18S rRNA and for 40S ribosome production; associated with spindle/microtubules; nuclear localization depends on physical interaction with Nop14p; may bind snoRNAs
1767;UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids, involved in autophagy
1768;Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p
1769;Dual function protein involved in translation initiation as a substoichiometric component of eukaryotic translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA
1770;Hypothetical protein
1771;N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
1772;Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
1773;Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
1774;Hypothetical protein
1775;Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
1776;Nuclear encoded protein required for translation of COX1 mRNA; binds to Cox1 protein
1777;Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events
1778;Component of both the Nup84 nuclear pore sub-complex and of the COPII complex (Sar1p, Sec13p, Sec16p, Sec23p, Sec24p, Sec31p, Sfb2p, and Sfb3p) which is important for the formation of ER to Golgi transport vesicles
1779;Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides
1780;Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels
1781;Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate
1782;Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation
1783;Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
1784;Hypothetical protein
1785;Hypothetical protein
1786;Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II
1787;Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
1788;Kynureninase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway
1789;DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination
1790;Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
1791;Protein required for normal intracellular sterol distribution and for sphingolipid metabolism; similar to Nup120p and C. elegans R05H5.5 protein
1792;Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
1793;Protein kinase involved in the response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations
1794;Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP
1795;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
1796;Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17
1797;Hypothetical protein
1798;homolog of nuclear distribution factor NudE, NUDEL
1799;GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
1800;Similar to C. elegans protein
【０１５４】
1801;Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Bp and has similarity to rat S28 ribosomal protein
1802;R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
1803;Non-essential hydrolase involved in mRNA decapping, may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p
1804;Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin and silencing at the mating type locus
1805;Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
1806;ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 60S ribosomal subunit
1807;Putative endonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs
1808;Putative nicotinamide N-methyltransferase, has a role in rDNA silencing and in lifespan determination
1809;Endochitinase, required for cell separation after mitosis; transcriptional activation during late G and early M cell cycle phases is mediated by transcription factor Ace2p
1810;DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
1811;Mitochondrial GTPase of unknown function, similar to E. coli elongation factor-type GTP-binding protein LepA and to LK1236.1 from Caenorhabditis elegans
1812;Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
1813;GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Prp20p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; yeast Gsp2p homolog
1814;Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
1815;Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
1816;Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes
1817;O-acetyl homoserine-O-acetyl serine sulfhydrylase, required for sulfur amino acid synthesis
1818;Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
1819;Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall1820;Identified by homology to Ashbya gossypii
1821;Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G11822;Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM
1823;Subunit of the RSC chromatin remodeling complex required for kinetochore function in chromosome segregation; essential gene required for cell cycle progression; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
1824;Hypothetical protein
1825;Hypothetical protein
1826;Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
1827;Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p localization, also involved in cell fusion during mating
1828;O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
1829;Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similarity to rat S25 ribosomal protein
1830;Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphorylated on serine 302
1831;Protein of unknown function; expression regulated by PDR1
1832;Karyopherin beta, forms a dimeric complex with Srp1p (Kap60p) that mediates nuclear import of cargo proteins via a nuclear localization signal (NLS), interacts with nucleoporins to guide transport across the nuclear pore complex
1833;Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
1834;Hypothetical protein
1835;Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
1836;Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
1837;Hypothetical protein
1838;One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; required for expression of mid-late sporulation-specific genes; contains two essential  bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
1839;Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway
1840;Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity
1841;Putative phosphoesterase that functions as a dosage-dependent positive regulator of the G1/S phase transition by controlling the timing of START
1842;Identified by expression profiling and mass spectrometry
1843;Protein interacting with Nam7p, may be involved in the nonsense-mediated mRNA decay pathway
1844;Hypothetical protein
1845;Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
1846;GDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEP
1847;Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles
1848;Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
1849;Phosphatidylinositol transfer protein with a potential role in lipid turnover; interacts specifically with thioredoxin peroxidase (Tsa2p) and may have a role in oxidative stress resistance
1850;Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
【０１５５】
1851;Protein involved in structural maintenance of chromosomes; required for interchromosomal and sister chromatid recombination; homologous to S. pombe rad18
1852;Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; activator of Fab1p
1853;Protein of unknown function, similar to Rei1p but not involved in bud growth; contains dispersed C2H2 zinc finger domains
1854;Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Bp and has similarity to rat S29 and E. coli S14 ribosomal proteins
1855;Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall
1856;Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6
1857;Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
1858;ATP-binding protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at both the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
1859;ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; may be involved in degradation of aberrant mRNAs
1860;Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p
1861;Hypothetical protein
1862;Protein of unknown function probably involved in the function of the cortical actin cytoskeleton; putative ortholog of S. pombe asp1+
1863;High-affinity copper transporter of the plasma membrane, acts as a trimer; gene is disrupted by a Ty2 transposon insertion in many laboratory strains of S. cerevisiae
1864;Hypothetical protein
1865;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, and bud
1866;Hypothetical protein
1867;Protein that interacts with and is required for activation of Apg1p protein kinase; involved in autophagy but not in the Cvt (cytoplasm to vacuole targeting) pathway
1868;Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
1869;Guanine nucleotide exchange factor (GEF) that functions to modulate Rhop1 activity as part of the cell integrity signaling pathway; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
1870;DNA-3-methyladenine glycosidase II that catalyzes of the hydrolysis of alkylated DNA
1871;Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity
1872;Nuclear protein, putative helicase required for processing of tRNAs, rRNAs, and small nuclear RNAs; potential Cdc28p substrate
1873;Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
1874;Non-essential protein of unknown function
1875;Hypothetical protein
1876;Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
1877;Mitochondrial ribosomal protein of the large subunit
1878;Protein of unknown function proposed to be involved in protein secretion
1879;Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein
1880;Subunit D of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found in the endomembrane system; stabilizes VO subunits; required for V1 domain assembly on the vacuolar membrane
1881;Nuclear protein, putative peptidyl-prolyl cis-trans isomerase (PPIase) with similarity to Fpr3p; overproduction suppresses the growth defect resulting from the absence of E3 ubiquitin-protein ligase Tom1p
1882;Zinc-finger transcription factor that regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; positively regulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis
1883;GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
1884;Hypothetical protein
1885;Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition
1886;GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
1887;Glycoprotein required for oxidative protein folding in the endoplasmic reticulum
1888;Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc1 mutants are defective in directing meiotic recombination events to homologous chromatids
1889;One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
1890;Essential protein required for maturation of Gas1p and Pho8p; GFP-fusion protein localizes to the endoplasmic reticulum; null mutants have a cell separation defect
1891;Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle, through its homolog and binding partner, Gtr2p; involved in phosphate transport and invasive growth; human RagA and RagB proteins are functional homologs
1892;NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I which is absent in S. cerevisiae
1893;Protein of unknown function, potential Cdc28p substrate
1894;Putative RNA-binding protein, based on computational analysis of large-scale protein-protein interaction data
1895;DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability
1896;Gamma subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing
1897;Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1
1898;2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; located in mitochondria
1899;Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; paralog of Zds1p
1900;Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose, homologous to Tps3p and may share function
【０１５６】
1901;Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
1902;A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
1903;Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
1904;Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
1905;Hypothetical protein
1906;D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of D-erythroascorbic acid, which is protective against oxidative stress
1907;Hypothetical protein
1908;Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
1909;Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17
1910;Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
1911;Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
1912;Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
1913;Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which facilitates RNA Polymerase II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure
1914;Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
1915;Protein of unknown function involved in the assembly of the prospore membrane during sporulation
1916;Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p
1917;GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis
1918;Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein
1919;Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA
1920;Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase
1921;Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions
1922;Hypothetical protein
1923;Integral membrane protein localized to cortical patch structures; multicopy suppressor of rvs167 mutation; mutants show defects in sporulation and altered sphingolipid content in plasma membrane
1924;Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding
1925;U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
1926;Protein required for rDNA transcription by RNA polymerase I, component of the core factor (CF) of rDNA transcription factor, which also contains Rrn6p and Rrn7p
1927;Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
1928;Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin; required for vacuolar protein sorting
1929;Putative nucleotide sugar transporter, has similarity to Vrg4p
1930;Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis
1931;Hypothetical protein
1932;Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate
1933;Adenine phosphoribosyltransferase, catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis
1934;Hypothetical protein
1935;Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
1936;Hypothetical protein
1937;TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
1938;UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, has a ubiquitin-associated (UBA) domain, interacts with ubiquitylated proteins in vivo, and is required for degradation of a ubiquitylated model substrate
1939;Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport
1940;Hypothetical protein
1941;Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36.
1942;Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; mediates pleiotropic drug and metal resistance; localized to the nucleus in response to the presence of oxidants
1943;CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway
1944;GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion, similar to mammalian Rab7
1945;Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate
1946;Hypothetical protein
1947;Hypothetical protein
1948;Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole
1949;TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
1950;Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1
【０１５７】
1951;eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant
1952;Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
1953;Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
1954;C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
1955;Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
1956;Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
1957;RING-finger peroxisomal membrane peroxin that plays an essential role in peroxisome biogenesis and peroxisomal matrix protein import, forms translocation subcomplex with Pex2p and Pex10p
1958;Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
1959;Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
1960;Protein required for respiratory growth; localized to both the nucleus and mitochondrion; mutant displays decreased transcription of specific nuclear and mitochondrial genes whose products are involved in respiratory growth
1961;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
1962;Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
1963;Transcriptional activator related to Msn4p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression
1964;Copper chaperone for superoxide dismutase Sod1p, involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation
1965;Transcriptional coactivator, facilitates elongation by influencing enzymes that modify RNAP II, acts in a peroxide resistance pathway involving Rad2p; suppressor of TFIIB mutations
1966;Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
1967;Member of a complex (Isw1b) with Isw1p and Ioc2p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PWWP motif
1968;Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p
1969;Component of the mitochondrial outer membrane sorting and assembly machinery (SAM) complex; required for the sorting of some proteins to the outer membrane after import by the TOM complex
1970;Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
1971;Protein of unknown function, involved in the proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans PalI; putative membrane protein
1972;UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p
1973;Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p
1974;Putative protein of unknown function; proposed to be involved in resistance to carboplatin and cisplatin; shares similarity to a human cytochrome oxidoreductase
1975;Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
1976;Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
1977;Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production
1978;Hypothetical protein
1979;Hypothetical protein
1980;Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats
1981;Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
1982;Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins
1983;Peripheral GTPase of the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
1984;Hypothetical protein
1985;Protein of unknown function, deletion causes multi-budding phenotype; has similarity to Aspergillus nidulans samB gene
1986;Phosphoglucomutase, catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase
1987;Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
1988;Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control
1989;Hypothetical protein
1990;Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
1991;Hypothetical protein
1992;Putative integral membrane E3 ubiquitin ligase; genetic interactions suggest a role in negative regulation of amino acid uptake
1993;Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade16p; ade16 ade17 mutants require adenine and histidine
1994;Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA
1995;Hypothetical protein
1996;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck
1997;Protein of unknown function, deletion causes sensitivity to thermal stress
1998;Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which  acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
1999;Hypothetical protein
2000;Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
【０１５８】
2001;Protein required for cell viability
2002;Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains LisH and CTLH domains, like Vid30p; dosage-dependent regulator of START
2003;Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
2004;Required for a post-incision step in the repair of DNA single and double-strand breaks that result from interstrand crosslinks produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation
2005;Protein kinase required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta
2006;Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase
2007;Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain
2008;Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation
2009;Hypothetical protein
2010;Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum2011;Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
2012;Protein of unknown function; proposed to be involved in responding to DNA damaging agents
2013;Subunit of the nuclear pore complex (NPC), interacts with karyopherin Kap121p or with Nup170p via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex
2014;The authentic, non-tagged protein was localized to the mitochondria
2015;Mitochondrial ribosomal protein of the small subunit
2016;Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the trans-Golgi, likely involved in protein transport
2017;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
2018;Protein required for mismatch repair in mitosis and meiosis, postmeiotic segregation, and spore viability; forms a complex with Pms1p and Msh2p to repair mismatched DNA; human homolog is associated with hereditary non-polyposis colon cancer
2019;Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
2020;Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
2021;Cytoplasmic proteinase A inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
2022;Protein of unknown function whose expression is induced by osmotic stress
2023;Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks
2024;Protein of unknown function; open reading frame may be part of a bicistronic transcript with RGM1
2025;Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
2026;Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock
2027;P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
2028;Mitochondrial ribosomal protein of the large subunit
2029;Protein that interacts with the cytoskeleton and is involved in chromatin organization and nuclear transport, interacts genetically with TCP1 and ICY2, required for viability in rich media of cells lacking mitochondrial DNA
2030;Hypothetical protein
2031;Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways2032;Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p
2033;Protein that may be involved in cell wall function; mutations in rot1 cause cell wall defects, suppress tor2 mutations, and are synthetically lethal with rot2 mutations
2034;C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
2035;Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
2036;Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance
2037;Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
2038;Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
2039;Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
2040;Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
2041;Putative 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall
2042;SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
2043;GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation
2044;One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1
2045;Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B
2046;Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved
2047;NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids
2048;TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
2049;Mitochondrial RNA polymerase sigma-like specificity factor required for promoter recognition, interacts with mitochondrial core polymerase Rpo41p, imported into mitochondria via a novel process requiring most of the Mtf1p sequence
2050;Protein required for the synthesis of both 18S and 5.8S rRNA; C-terminal region is crucial for the formation of 18S rRNA and N-terminal region is required for the 5.8S rRNA; component of small ribosomal subunit (SSU) processosome
【０１５９】
2051;Ribonuclease H1, removes RNA primers during Okazaki fragment synthesis; degrades RNA attached to the 5'-end of a DNA strand
2052;GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
2053;Protein that colocalizes with clathrin-coated vesicles; involved in transport at the trans-Golgi
2054;Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
2055;RNAase III; cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end
2056;Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
2057;Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; also has a role in resistance to zinc shock resulting from a sudden influx of zinc into the cytoplasm
2058;Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
2059;Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor
2060;Hypothetical protein
2061;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
2062;Coiled-coiled protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation
2063;Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
2064;Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
2065;Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway
2066;Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate
2067;Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
2068;One of two orotate phosphoribosyltransferase isozymes (see also URA5) that catalyze the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate
2069;Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
2070;Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone
2071;Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome
2072;Hypothetical protein
2073;Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
2074;Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
2075;ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
2076;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud
2077;Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
2078;Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
2079;Mitochondrial inner membrane transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol; member of the ATP-binding cassette (ABC) transporter family
2080;Histone deacetylase; regulates transcription and silencing
2081;Protein required for efficient mating, member of a family of eukaryotic proteins that contain a domain homologous to Sac1p
2082;Protein of unknown function, potential Cdc28p substrate
2083;Hypothetical protein
2084;Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose
2085;Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
2086;Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase
2087;Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
2088;Protein required for cell viability
2089;F-box protein
2090;Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
2091;Hypothetical protein
2092;Hypothetical protein
2093;Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p
2094;Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)
2095;N-glycosylated integral membrane protein of the ER membrane and plasma membrane, required for Ca2+ influx stimulated by pheromone, functions as a stretch-activated Ca2+-permeable cation channel in mammals; forms an oligomer
2096;Pho85 cyclin of the Pcl1,2-like subfamily, involved in entry into the mitotic cell cycle and regulation of morphogenesis, localizes to sites of polarized cell growth
2097;Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
2098;Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
2099;Heat shock protein regulator that binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus
2100;C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions
【０１６０】
2101;L-homoserine-O-acetyltransferase, catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway
2102;Putative hydroxyisocaproate dehydrogenase
2103;Guanyl-nucleotide exchange factor for the small G-protein Sec4p, located on cytoplasmic vesicles; essential for post-Golgi vesicle transport
2104;Basic amino acid transporter, involved in uptake of cationic amino acids
2105;Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
2106;Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokineses through the actin cytoskeleton
2107;Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles
2108;Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
2109;Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake
2110;Putative zinc finger protein with similarity to human CNBP, proposed to be involved in the RAS/cAMP signaling pathway
2111;Protein involved in mRNA export, component of the transcription export (TREX) complex
2112;Mitochondrial ribosomal protein of the large subunit
2113;RNA-binding protein that interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), required for transcription termination and 3' end maturation of nonpolyadenylated RNAs
2114;RNA polymerase I subunit A49
2115;Component of a complex containing Cef1p, involved in pre-mRNA splicing; has similarity to S. pombe Cwf25p
2116;Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
2117;Transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization; adaptor protein that links actin to clathrin and endocytosis; present in the actin cortical patch of the emerging bud tip; dimer in vivo
2118;Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
2119;Similar to globins and has a functional heme-binding domain; involved in glucose signaling or metabolism; regulated by Rgt1p
2120;Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p
2121;Elongin A, F-box protein that forms a heterodimer with Elc1p and participates in transcription elongation
2122;Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration
2123;Hypothetical protein
2124;Transcription/RNA-processing factor that associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 of the RNA polymerase II C-terminal domain; affects start site selection in vivo
2125;Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase P
2126;Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
2127;Hypothetical protein
2128;DNA-binding protein involved in either activation or repression of transcription, depending on binding site context; also binds telomere sequences and plays a role in telomeric position effect (silencing) and telomere structure
2129;Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions
2130;Hypothetical protein
2131;Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
2132;Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
2133;Protein with a role in regulation of Ty1 transposition
2134;Hypothetical protein; similarity to human TGR-CL10C, thyroidal receptor for N-acetylglucosamine
2135;Hypothetical protein
2136;Hypothetical protein
2137;Ubiquitin-specific protease that deubiquitinates ubiquitin-protein moieties; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; primarily located in the nucleus
2138;Mitochondrial ribosomal protein of the large subunit
2139;Protein required for cell viability
2140;Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity
2141;Hypothetical protein
2142;Protein of unknown function, localizes to the nucleolus and nucleoplasm; contains an RNA recognition motif (RRM) and has similarity to Nop12p, which is required for processing of pre-18S rRNA
2143;Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations
2144;Phosphatidylserine decarboxylase of the mitochondrial inner membrane, converts phosphatidylserine to phosphatidylethanolamine
2145;The authentic, non-tagged protein was localized to mitochondria
2146;Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family that forms a complex with Tup1p and Ssn6p to both activate and repress transcription; cytosolic and nuclear protein involved in the osmotic and oxidative stress responses
2147;Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
2148;Hypothetical protein
2149;Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36.
2150;Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separation
【０１６１】
2151;Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p
2152;Predicted membrane protein; genetic interactions suggest a role in negative regulation of amino acid uptake
2153;Hypothetical protein
2154;Protein of unknown function, potential homolog of mammalian Insig 1
2155;Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck1p
2156;Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
2157;Essential protein required for maturation of Gas1p and Pho8p; GFP-fusion protein localizes to the endoplasmic reticulum (ER) and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
2158;Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
2159;Hypothetical protein
2160;Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
2161;Adenine deaminase (adenine aminohydrolase), involved in purine salvage and nitrogen catabolism
2162;CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis
2163;Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A
2164;Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function
2165;Essential protein of unknown function; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
2166;Component of the TOM (translocase of outer membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between the TOM and TIM complexes
2167;Nicotinamide riboside kinase, catalyzes the synthesis of nicotinamide nucleotide (NMN) from nicotinamide riboside; involved in a salvage pathway for NAD+ biosynthesis
2168;Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p
2169;Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
2170;shows protein sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
2171;Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
2172;Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex, which removes the 5' cap structure from mRNAs prior to their degradation; member of the Nudix hydrolase family
2173;Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium
2174;Protein involved in regulating spindle position and orientation, functionally redundant with Dma1p; homolog of S. pombe Dma1 and H. sapiens Chfr
2175;Hypothetical protein
2176;RNA polymerase subunit, common to RNA polymerases I and III
2177;Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; required for sterol C5-6 and fatty acid desaturation
2178;Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
2179;Hypothetical protein
2180;Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in endocytosis; hyperosmotic stress causes translocation to actin patches
2181;Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
2182;Hypothetical protein
2183;Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
2184;GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
2185;Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; C-terminus has similarity to human candidate tumor suppressor p33(ING1)
2186;Hypothetical protein
2187;Protein of unknown function, interacts with Rvs161p and Rvs167p; computational analysis of protein-protein interactions in large-scale studies suggests a possible role in actin filament organization
2188;GTPase, similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis
2189;Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
2190;Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly
2191;Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis
2192;Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud via the mRNA transport system involving She2p
2193;Protein that forms a complex with Pbp1p that may mediate posttranscriptional regulation of HO endonuclease; involved in propagation of M2 dsRNA satellite of L-A virus
2194;EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
2195;Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein
2196;Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently
2197;Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
2198;Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria
2199;Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis; cooperates with Rad27p nuclease
2200;Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
【０１６２】
2201;Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
2202;Transcription factor of the forkhead family that regulates the cell cycle and pseudohyphal growth; also involved in chromatin silencing at HML and HMR; potential Cdc28p substrate
2203;Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Ap and has similarity to E. coli L6 and rat L9 ribosomal proteins
2204;Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein translocation across membranes; member of the DnaJ family
2205;Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
2206;Integral vacuolar membrane protein involved in vacuole inheritance and morphology; may function to regulate Fab1p kinase activity
2207;Subunit Va of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth
2208;Probable component of COPII coated vesicles that binds to Sec23p; similar to and functionally redundant with Sec24p, but expressed at low levels; involved in ER to Golgi transport and in autophagy
2209;Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
2210;Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
2211;Hypothetical protein
2212;Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
2213;One of two identical histone H3 proteins (see also HHT1); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation2214;One of two identical histone H4 proteins (see also HHF1); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
2215;Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
2216;Transcription factor that activates transcription of genes involved in stress response; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation
2217;Essential component of the Sorting and Assembly Machinery (SAM or Tob complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
2218;Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
2219;Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2220;Putative catalytic subunit of a class II histone deacetylase complex that also contains Hda2p and Hda3p; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation
2221;Serine/threonine protein kinase involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis
2222;Cytosolic inhibitor of vacuolar proteinase B, required for efficient vacuole inheritance; with thioredoxin forms protein complex LMA1, which assists in priming SNARE molecules and promotes vacuole fusion
2223;Hypothetical protein
2224;Hypothetical protein
2225;Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids
2226;Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins
2227;Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
2228;Mitochondrial ribosomal protein of the large subunit
2229;Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Gbp2p and Npl3p
2230;Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein
2231;Putative transmembrane protein, involved in the export of ammonia, a starvation signal that promotes cell death in the center of aging colonies; member of the TC 9.B.33 YaaH family; homolog of Ady2p and Y. lipolytica Gpr1p
2232;RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
2233;Hypothetical protein
2234;Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
2235;Protein involved in autophagy; E2-like enzyme that plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy
2236;Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
2237;Putative transcriptional repressor of MBF (MCB binding factor) target genes2238;Component of the Med9/10 module, which is a subcomplex within the RNA polymerase II Mediator complex; required for regulation of RNA polymerase II activity2239;Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
2240;Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
2241;Essential protein of the mitochondrial inner membrane, component of the mitochondrial import system
2242;Hypothetical protein
2243;Acyl-CoA:sterol acyltransferase, isozyme of Are1p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen
2244;Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation
2245;73 kDa subunit of the 11-subunit SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive
2246;Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
2247;Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway
2248;Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
2249;Putative serine/threonine protein phosphatase, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
2250;Ubiquitin-like protein modifier, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
【０１６３】
2251;Para-aminobenzoate (PABA) synthase, has similarity to Escherichia coli PABA synthase components PabA and PabB
2252;Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol2p, Sol3p, and Sol4p
2253;Hypothetical protein
2254;Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin2255;Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein
2256;Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
2257;Endoplasmic reticulum protein of unknown function, transcription is induced under conditions of zinc deficiency; mutant phenotype suggests a role in uptake of zinc
2258;Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
2259;Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer2260;Specific translational activator for the COX3 mRNA that acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
2261;Subunit of an adoMet-dependent tRNA methyltransferase (MTase) complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; putative zinc binding subunit of other MTase-related proteins
2262;Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate
2263;Protein proposed to interact with phospholipid translocases, shares similarity to Cdc50p
2264;Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
2265;RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
2266;Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator
2267;Putative ion transporter similar to the major facilitator superfamily of transporters; mutations in membrane-spanning domains permit nonselective cation uptake
2268;7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
2269;Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation
2270;Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother
2271;Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36.
2272;Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
2273;Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH
2274;Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin
2275;Protein involved in rRNA processing; component of the exosome 3->5 exonucle
ase complex
2276;Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
2277;Cytoplasmic mRNA cap binding protein; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with the translation initiation factor eIF4G (Tif4631p or Tif4632p)
2278;Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
2279;RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF2280;Hypothetical protein
2281;Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
2282;Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria
2283;Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation
2284;Hypothetical protein
2285;Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain
2286;Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences2287;Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
2288;Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
2289;Transcriptional activator involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus
2290;Catalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), a nuclear poly (A) polymerase complex involved in RNA quality control; catalyzes polyadenylation of unmodified tRNAs, and snoRNA and rRNA precursors; disputed role as a DNA polymerase
2291;Hypothetical protein
2292;Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; proposed to be a downstream effector of Cdc42p during polarized growth
2293;Nuclear protein required for efficient mating, involved in shmoo formation and nuclear migration in the pre-zygote, contains a ubiquitin-like (UBL) domain
2294;Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p
2295;Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
2296;Hypothetical protein
2297;Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution (interference), sister chromatid cohesion at meiotic telomeres, and segregation of small chromosomes
2298;Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
2299;Putative metalloprotease
2300;Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription
【０１６４】
2301;Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
2302;Protein that forms heterodimers, with Msh3p and Msh6p, that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity; Msh2p-Msh6p binds to and hydrolyzes ATP
2303;Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression
2304;Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
2305;Hypothetical protein
2306;Protein of unknown function, expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole
2307;Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly
2308;Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase
2309;Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
2310;Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met; involved in mitochondrial inheritance and actin assembly
2311;Hypothetical protein
2312;Hypothetical protein
2313;Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
2314;Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus
2315;Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
2316;Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway
2317;Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport
2318;Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport
2319;5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a five related enzymes, which are active as heteromultimeric complexes
2320;Protein required for normal mitochondrial morphology, has similarity to hemolysins
2321;NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria
2322;Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
2323;Putative metalloprotease
2324;Hydroxymethylpyrimidine phosphate kinase, involved in the last steps in thiamine biosynthesis; member of a gene family with THI21 and THI22; functionally redundant with Thi21p
2325;Nuclear protein, putative RNA polymerase II elongation factor; isolated as Pob3p/Spt16p-binding protein
2326;Multistress response protein, expression is activated by a variety of xenobiotic agents and environmental or physiological stresses
2327;Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly
2328;DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus
2329;Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p
2330;Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ribosomal proteins
2331;Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
2332;20S proteasome alpha-type subunit
2333;Protein of unknown function; potential Cdc28p substrate
2334;Mitochondrial glutamyl-tRNA synthetase, encoded by a nuclear gene
2335;ORF, Uncharacterized
2336;Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein
2337;Putative 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall
2338;Mitochondrial inner membrane protein, required for K+/H+ exchange and for normal mitochondrial morphology and inheritance; human ortholog Letm1 is implicated in Wolf-Hirschhorn syndrome
2339;Mitochondrial outer membrane protein, required for assembly of the translocase of the outer membrane (TOM) complex and thereby for mitochondrial protein import; N terminus is exposed to the cytosol: transmembrane segment is highly conserved
2340;Mitochondrial translation initiation factor 2
2341;Protein required for cell viability
2342;High affinity tryptophan and tyrosine permease, overexpression confers FK506 resistance
2343;Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation
2344;Hypothetical protein
2345;Ubiquitin-protein ligase required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger
2346;Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
2347;Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro
2348;RNA terminal phosphate cyclase-like protein involved in rRNA processing at sites A0, A1, and A2; does not possess detectable RNA cyclase activity
2349;Appears to be a structural component of the chitin synthase 3 complex
2350;Palmitoyltransferase with autoacylation activity; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
【０１６５】
2351;Cyclin, negatively regulates phosphate metabolism; Pho80p-Pho85p (cyclin-CDK complex) phosphorylates Pho4p and Swi5p; deletion of PHO80 leads to aminoglycoside supersensitivity; truncated form of PHO80 affects vacuole inheritance
2352;Glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
2353;Putative precursor to the subtilisin-like protease III
2354;Glutamine-rich cytoplasmic protein of unknown function, contains tetratricopeptide (TPR) repeats, which often mediate protein-protein interactions; conserved in human and C. elegans
2355;Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
2356;B-type regulatory subunit of protein phosphatase 2A (PP2A); homolog of the mammalian B' subunit of PP2A
2357;Identified by homology to Ashbya gossypii
2358;Hypothetical protein
2359;Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex
2360;Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p
2361;Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones; activates the ATPase activity of Ssa1p; homolog of mammalian Hop protein2362;Probable multiple transmembrane protein, involved in invasive growth upon nitrogen starvation; required for accumulation of processed Rim101p
2363;Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
2364;Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
2365;Beta' regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB1, the many substrates include transcription factors and all RNA polymerases
2366;Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression
2367;Hypothetical protein
2368;Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
2369;Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family involved in mRNA export from the nucleus
2370;Protein involved in control of glucose-regulated gene expression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt15p; acts as a regulator of the transcription factor Rgt1p
2371;Hypothetical protein
2372;Essential nuclear protein involved in proteasome maturation and synthesis of 40S ribosomal subunits; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA
2373;Probable cochaperone, regulates activity of Cyr1p (adenylyl cyclase); involved in assembly of the kinetochore complex, associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex
2374;Protein required for cell viability
2375;Alpha' catalytic subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKB1 and CKB2, the many substrates include transcription factors and all RNA polymerases
2376;Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p
2377;Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribosomal proteins; involved in the replication and maintenance of killer double stranded RNA virus
2378;Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; has similarity to the human tumor suppressor ING1
2379;Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
2380;Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability
2381;Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
2382;Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
2383;Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation
2384;GTPase required for transport during endocytosis and for correct sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog
2385;Mitochondrially localized type 2C protein phosphatase; contains Mg2+/Mn2+-dependent casein phosphatase activity in vitro but in vivo substrates are unknown2386;Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity
2387;Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
2388;Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
2389;Hypothetical protein
2390;Nuclear pore complex (NPC) subunit, involved in protein import/export and in export of RNAs, possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; potential Cdc28p substrate
2391;Alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
2392;Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation
2393;GTPase involved in G-protein signaling in the adenylate cyclase activating pathway, plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes
2394;Protein that induces appearance of [PIN+] prion when overproduced
2395;Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in a TGN (trans Golgi network)-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches
2396;One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes
2397;Hypothetical protein
2398;Hypothetical protein
2399;One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
2400;RNA polymerase III subunit C160, part of core enzyme; similar to bacterial beta-prime subunit
2401;One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription
2402;Hypothetical protein
2403;Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA
2404;Putative NADP(+) coupled glycerol dehydrogenase, proposed to be involved in an alternative pathway for glycerol catabolism
2405;Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing
2406;Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine
2407;Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
2408;Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation
2409;Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
2410;Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
2411;Protein required for S phase progression and telomere homeostasis, forms an alternative replication factor C complex important for DNA replication and genome integrity; mutants are sensitive to DNA damage
2412;Essential nucleolar protein required for pre-18S rRNA processing, interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain
2413;RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
2414;Hypothetical protein
2415;Short-lived membrane ABC (ATP-binding cassette) transporter, actively exports various drugs, expression regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth
2416;Hypothetical protein
2417;Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
2418;SUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins
2419;Endopeptidase with trypsin-like activity that cleaves after basic residues; beta-type subunit of 20S proteasome synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
2420;Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
2421;Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
2422;Hypothetical protein
2423;Hypothetical protein
2424;Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Ap and has similarity to rat S28 ribosomal protein
2425;Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
2426;Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
2427;Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
2428;Member of the MBOAT family of putative membrane-bound O-acyltransferases
2429;Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Yfh1p mediates the use of iron by Hem15p
2430;Peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, involved in fatty acid metabolism, contains peroxisome targeting signals at amino and carboxy termini
2431;Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
2432;3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p
2433;GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability
2434;Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans
2435;Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions
2436;Member of the SWI/SNF family of DNA-dependent ATPases, plays a role in antagonizing silencing during mating-type switching, contains an N-terminal domain that interacts with Sir4p and a C-terminal SNF2 domain
2437;TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
2438;Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
2439;Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
2440;Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
2441;ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
2442;The authentic, non-tagged protein was localized to the mitochondria
2443;RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III2444;Hypothetical protein
2445;Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
2446;Hypothetical protein
2447;RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
2448;Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable
2449;Hypothetical protein
2450;Transcriptional repressor involved in regulation of meiosis and silencing; contains WD repeats
【０１６６】
2451;Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p
2452;Protein of the mitochondrial matrix involved in protein import into mitochondria; acts as a cochaperone and a nucleotide release factor for Ssc1p; homolog of E. coli GrpE
2453;Nonessential protein kinase with unknown cellular role
2454;Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function
2455;Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP)
2456;Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis
2457;Pseudouridine synthase, catalyzes pseudouridylation at position 35 in U2 snRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); Asp(256) mutation abolishes activity; conserved in archaea, some bacteria, and vertebrates
2458;Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression
2459;Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
2460;Protein with similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
2461;Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
2462;Subunit of cleavage factor I (CFI), involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
2463;catalyzes transfer of the sulfane atom of thiosulfate to cyanide to form sulfite and thiocyanate
2464;Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
2465;Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER2466;Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p
2467;Member of the third branch of the histidine triad (HIT) superfamily of nucleotide-binding proteins; similar to Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia
2468;One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for spindle pole body duplication; localized mainly to the nucleus throughout the cell cycle
2469;Protein required for cell viability
2470;Protein involved in microtubule morphogenesis, required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin
2471;Protein kinase implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis
2472;Hypothetical protein
2473;Constituent of 66S pre-ribosomal particles, required for maturation of the large ribosomal subunit
2474;Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase, required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms
2475;Protein involved in proteolytic activation of Rim101p in response to alkaline pH; member of the PalA/AIP1/Alix family; interacts with the ESCRT-III subunits Snf7p, suggesting a relationship between the response to pH and multivesicular body formation
2476;DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p
2477;Essential protein with similarity to phosducins, which are GTPase inhibitors; lethality of null mutation is functionally complemented by expression of mouse phosducin-like protein MgcPhLP
2478;Hypothetical protein
2479;Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
2480;Hypothetical protein
2481;The authentic, non-tagged protein was localized to the mitochondria
2482;Member of the protein disulfide isomerase (PDI) family; overexpression suppresses the defect in maturation of carboxypeptidase Y, and defects in other essential Pdi1p functions, caused by PDI1 deletion
2483;Catalytic subunit of the 11-subunit SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
2484;Hypothetical protein
2485;Hypothetical protein
2486;Essential protein that binds ribosomal protein L11 and is required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; mouse homolog shows altered expression in Huntington's disease model mice
2487;Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane; may function to stabilize the complex
2488;Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acetyltransferases
2489;Protein involved in bud-site selection; diploid mutants display an axial-like budding pattern
2490;CPA1 uORF , Arginine attenuator peptide, regulates translation of the CPA1 mRNA
2491;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck
2492;Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
2493;Protein involved in ER-to-Golgi transport
2494;U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
2495;N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus
2496;Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface
2497;One of two type V myosins, involved in polarized distribution of mitochondria; required for mitochondrion and vacuole inheritance and nuclear spindle orientation; moves multiple cargo
2498;Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins
2499;Protein required for normal cortical actin organization and endocytosis; multicopy suppressor of clathrin deficiency; acts as a targeting subunit for protein phosphatase type 1
2500;Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
【０１６７】
2501;Cytoplasmic alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
2502;Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
2503;Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein
2504;RNA polymerase I subunit A43
2505;RNA polymerase I subunit; largest subunit of RNA polymerase I
2506;Hypothetical protein
2507;Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression
2508;Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells
2509;Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
2510;Protein required for cell morphogenesis and cell separation after mitosis
2511;Protein of unknown function, required for growth on glycerol as a carbon source
2512;Hypothetical protein
2513;Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
2514;Protein of unknown function, shows genetic interactions with Vti1p, which is a v-SNARE involved in cis-Golgi membrane traffic
2515;20S proteasome alpha-type subunit
2516;Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes
2517;Hypothetical protein
2518;Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins
2519;Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12
2520;Rab escort protein, forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
2521;Proposed beta subunit of the heterotrimeric G protein that interacts with the receptor Grp1p, has signaling role in response to nutrients; involved in regulation of pseudohyphal growth through cAMP levels; homolog of Gpb2p
2522;Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
2523;Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed
2524;NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources
2525;Hypothetical protein
2526;Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins
2527;Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
2528;Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
2529;Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
2530;DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p
2531;Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
2532;Hypothetical protein
2533;Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family
2534;Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen
2535;Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
2536;Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; forms a complex, with t-SNARE Sec9p, that binds v-SNARE Snc2p; also required for sporulation; syntaxin homolog that is functionally redundant with Sso2p
2537;Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities
2538;Hypothetical protein
2539;Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
2540;UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum
2541;ATP binding cassette family member; Asn/Gln-rich rich region supports [NU+] prion formation, susceptibility to [PSI+] prion induction and aggregation of a fragment of the human Machado-Joseph Disease protein
2542;Hypothetical protein
2543;Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation
2544;Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p
2545;Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
2546;Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
2547;SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit protein L23a (RPL23A and RPL23B)
2548;Endoplasmic reticulum protein of unknown function; contains glycerophosphodiester phosphodiesterase motifs but mutation does not detectably affect glycerophosphoinositol or glycerophosphocholine metabolism
2549;Protein kinase involved in regulating diverse events including vesicular trafficking, gene expression, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
2550;Subunit of cytoplasmic cAMP-dependent protein kinase, which contains redundant catalytic subunits Tpk1p, Tpk2p, and Tpk3p and regulatory subunit Bcy1p; promotes vegetative growth in response to nutrients; activates filamentous growth
【０１６８】
2551;Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
2552;Protein required for accurate chromosome segregation during meiosis
2553;Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes but not in prokaryotes
2554;DNA damage checkpoint protein, part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate
2555;Hypothetical protein
2556;Identified by homology to Ashbya gossypii
2557;Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog
2558;Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor
2559;Homolog of the prokaryotic ribosomal protein L36, likely to be a mitochondrial ribosomal protein coded in the nuclear genome
2560;Hypothetical protein
2561;Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif
2562;Homeodomain-containing transcriptional repressor of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription
2563;Plasma membrane localized protein of unknown function; contains sequence similarity to the transferrin receptor
2564;Mitochondrial ribosomal protein of the large subunit
2565;Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
2566;Protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
2567;Cell wall and vacuolar protein, required for wild-type resistance to vanadate
2568;Hypothetical protein
2569;Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
2570;Protein required for respiratory growth and stability of the mitochondrial genome
2571;Trimethyl guanosine synthase, conserved nucleolar methyl transferase responsible for conversion of the m(7)G cap structure of snRNAs and snoRNAs to m(2,2,7)G; also required for ribosome synthesis and nucleolar morphology
2572;Pheromone-regulated protein, predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift
2573;Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; synthesized as a zymogen, self-activates
2574;Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p
2575;Activator of the phosphotyrosyl phosphatase activity of PP2A; regulates G1 phase progression, the osmoresponse and microtubule dynamics; implicated in the spindle assembly check; subunit of the Tap42p-Pph21p-Rrd2p complex
2576;Splicing factor that is found in the Cef1p subcomplex of the spliceosome
2577;Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins
2578;Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
2579;Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex2580;Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein
2581;Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
2582;Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2583;Zinc cluster protein involved in conferring resistance to ketoconazole
2584;Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly
2585;Meiosis-specific protein of unknown function, involved in completion of nuclear divisions; identified as a weak high-copy suppressor of the spo1-1 ts mutation; putative GPI-dependent cell-wall protein
2586;Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation
2587;U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
2588;Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
2589;Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
2590;Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs
2591;Mitochondrial ribosomal protein of the large subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
2592;Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
2593;Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
2594;Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
2595;Putative dehydrogenase
2596;Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p
2597;Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance
2598;Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate after its uptake by Git1p permease, for use as a phosphate source and as a precursor for phosphocholine synthesis
2599;Hypothetical protein
2600;Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
【０１６９】
2601;The authentic, non-tagged protein was localized to the mitochondria
2602;Elongator protein, part of the HAP subcomplex of Elongator, which is a six-subunit component of the RNA polymerase II holoenzyme; required for Elongator structural integrity and histone acetyltransferase activity
2603;The authentic, non-tagged protein was localized to the mitochondria
2604;Protein required for growth of cells lacking the mitochondrial genome
2605;Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis
2606;Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
2607;Cytosolic and mitochondrial glutathione oxidoreductase, converts oxidized glutathione to reduced glutathione
2608;MADS-box transcription factor, component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p
2609;Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity
2610;Histone acetyltransferase subunit of the Elongator complex, which is a component of the RNA polymerase II holoenzyme; activity is directed specifically towards histones H3 and H4; disruption confers resistance to K. lactis zymotoxin
2611;Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
2612;Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
2613;Protein of unknown function, localized to the cytoplasm; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation
2614;Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
2615;Hypothetical protein
2616;Hypothetical protein
2617;Hypothetical protein
2618;Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; involved in transport of precursors for soluble vacuolar hydrolases from the late endosome to the vacuole
2619;Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p
2620;Constituent of the mitochondrial inner membrane presequence translocase (TIM23 complex); may promote binding of incoming precursor proteins to the intermembrane space domain of Tom22p during translocation
2621;Cytosolic aldehyde dehydrogenase that is activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for the conversion of acetaldehyde to acetate; constitutively expressed
2622;Protein of unknown function; null mutant forms abnormally large cells
2623;GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
2624;Subunit of Golgi mannosyltransferase complex also containing Anp1p, Mnn10p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation
2625;Translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, may play a redundant role in the regulation of protein synthesis or another GTP-dependent process
2626;Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
2627;Elongin C, forms heterodimer with Ela1p that participates in transcription elongation; expression dramatically upregulated during sporulation; widely conserved among eukaryotes
2628;Subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; part of the Class C Vps complex essential for membrane docking and fusion at both the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
2629;Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
2630;Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
2631;Hypothetical protein
2632;Hypothetical protein
2633;Zinc-finger DNA-binding protein, involved in regulating expression of the methionine biosynthetic genes, similar to Met32p
2634;Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b
2635;Protein of unknown function, mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck
2636;Hypothetical protein
2637;Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
2638;Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway
2639;Involved in response to DNA damage; null mutants have increased rates of recombination and delayed S phase; interacts physically and genetically with Sgs1p (RecQ family member) and Top3p (topoisomerase III)
2640;Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
2641;Ubl (ubiquitin-like protein)-specific protease that cleaves Smt3p protein conjugates;  specifically required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase
2642;Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion
2643;Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly
2644;One of 11 subunits of the SWI/SNF chromatin remodeling complex that regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2
2645;Hypothetical protein
2646;Mitochondrial ribosomal protein of the small subunit
2647;Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; contains HEAT-repeats
2648;Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
2649;Peripheral mitochondrial inner membrane protein, located on the matrix face of the membrane; stabilizes the bicistronic AAP1-ATP6 mRNA encoding subunits 6 and 8 of the ATP synthase complex
2650;Long chain base-responsive inhibitor of protein kinases Pkh1p and Pkh2p, acts along with Pil1p to down-regulate heat stress resistance via regulation of the Pkc1p and Ypk1p pathways; phosphorylated by Phk1p and Phk2p
【０１７０】
2651;Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation
2652;Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
2653;Hypothetical protein
2654;Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p
2655;Transcriptional activator involved in the transcription of TPO2, HSP30 and other genes encoding membrane stress proteins; despite sequence similarity with the transcription factor Ace1p, it is not subject to metalloregulation
2656;Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
2657;Hypothetical protein
2658;Hypothetical protein
2659;Hypothetical protein
2660;Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
2661;Nucleotide release factor functioning in the post-Golgi secretory pathway, required for ER-to-Golgi transport, binds zinc, found both on membranes and in the cytosol; guanine nucleotide dissociation stimulator
2662;Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt the DNA prior to replication; accumulates in the nucleus in G1; homolog of S. pombe Cdc21p
2663;Subunit g of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase
2664;Hypothetical protein
2665;Subunit, with Mgr1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; mutation causes an elevated rate of mitochondrial turnover
2666;Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
2667;Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro77p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p
2668;Cytoplasmic and mitochondrial histidine tRNA synthetase; encoded by a single nuclear gene that specifies two messages; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence 
2669;Glutamine synthetase (GS), synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation
2670;Protein that interacts physically and genetically with Tap42p, which regulates protein phosphatase 2A; component of the TOR (target of rapamycin) signaling pathway
2671;Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
2672;Hypothetical protein
2673;Protein involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA
2674;Protein with a potential role in DNA replication; displays synthetic lethal genetic interaction with the pol3-13 allele of POL3, which encodes DNA polymerase delta
2675;Peripheral membrane protein required for delivery of aminopeptidase I (Lap4p) to the vacuole in the cytoplasm-to-vacuole targeting pathway; also required for peroxisomal degradation (pexophagy)
2676;High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Bp; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters
2677;Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
2678;Putative mitochondrial inner membrane transporter, member of the mitochondrial carrier (MCF) family
2679;Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
2680;Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
2681;Heme-dependent repressor of hypoxic genes; contains an HMG domain that is responsible for DNA bending activity
2682;Putative class I histone deacetylase (HDAC) with sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; deletion results in increased histone acetylation at rDNA repeats; interacts with the Tup1p-Ssn6p corepressor complex
2683;Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
2684;Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
2685;Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
2686;Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
2687;Transketolase, similar to Tkl2p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids
2688;Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes
2689;17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of the human U5-specific 15-kDa protein
2690;Protein required for normal mitochondrial morphology and inheritance
2691;Hypothetical protein
2692;Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
2693;Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
2694;Guanine nucleotide exchange factor (GEF) for Arf proteins; involved in vesicular transport; suppressor of ypt3 mutations; member of the Sec7-domain family
2695;Hypothetical protein
2696;Hypothetical protein
2697;Mitochondrial ribosomal protein of the large subunit
2698;Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Bp; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11
2699;20S proteasome beta-type subunit, responsible for the chymotryptic activity of the proteasome
2700;Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
【０１７１】
2701;Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
2702;Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
2703;Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis
2704;Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
2705;Hypothetical protein
2706;B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
2707;Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
2708;Hypothetical protein
2709;Protein containing an Lsm domain, may bind RNA and have a role in RNA processing; overproduction suppresses a null mutation in CHC1, which encodes the heavy chain of clathrin
2710;Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
2711;Small mitochondrial outer membrane protein crucial to a binding relay for the import of proteins into mitochondria; subunit on the outer mouth of the TOM channel that accepts precursors from the receptors Tom20p and Tom22p
2712;Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
2713;Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease
2714;Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; similar to human tafazzin, which is implicated in Barth syndrome
2715;Nucleolar protein, constituent of pre-60S ribosomal particles; required for processing of the 27S pre-rRNA at the A2 site to yield 5.8S and 25S rRNA
2716;Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway
2717;Hypothetical protein
2718;Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs)
2719;Protein that induces appearance of [PIN+] prion when overproduced
2720;Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
2721;Polyamine transport protein specific for spermine; localizes to the plasma membrane; member of the major facilitator superfamily
2722;Hypothetical protein
2723;Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functionally redundant with Skn1p
2724;Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway
2725;Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p, which is the largest subunit of RNA polymerase II; regulated by Cak1p
2726;Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
2727;Translation initiation factor eIF-4B, has RNA annealing activity; contains an RNA recognition motif and binds to single-stranded RNA
2728;Protein likely involved in protection against replication-dependent DNA damage; mutants are sensitive to methyl methanesulfonate (MMS); implicated in regulation of Ty1 transposition
2729;GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
2730;Mitochondrial ribosomal protein of the small subunit
2731;3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism
2732;Adapter that links synaptojanins Inp2p and Inp53p to the cortical actin cytoskeleton
2733;Protein of unknown function, transcriptionally activated by Yrm1p along with genes involved in multidrug resistance
2734;AAA-type ATPase required for efficient late endosome to vacuole transport; catalyzes the release of an endosomal membrane-associated class E VPS protein complex; cytoplasmic protein that is also associated with an endosomal compartment2735;Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate
2736;Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
2737;Splicing factor, component of the U4/U6-U5 snRNP complex
2738;GTPase-activating protein; component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat, involved in ER to Golgi transport and autophagy; stimulates the GDP-bound form of Sar1p
2739;Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
2740;Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
2741;Glycogen debranching enzyme containing glucanotranferase and alpha-1,6-amyloglucosidase activities, required for glycogen degradation
2742;Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
2743;Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski2p and Ski8p; required for repressing propagation of dsRNA viruses
2744;RNA polymerase III subunit C82
2745;Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide
2746;Hypothetical protein
2747;Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing
2748;Cytochrome c heme lyase (holocytochrome c synthase), attaches heme to apo-Cyc1p in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS)
2749;Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends
2750;Ortholog of human transcriptional coactivator SKIP, can activate transcription of a reporter gene; interacts with splicing factors Prp22p and Prp46p
【０１７２】
2751;Hypothetical protein
2752;Protein with a potential role in regulatory interactions between microtubules and the cell cycle, as suggested by genetic and physical interactions with Nap1p and genetic interactions with TUB1
2753;Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p
2754;Hypothetical protein
2755;Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
2756;Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways
2757;Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
2758;Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain
2759;Protein required for autophagy; modified by the serial action of Atg4p, Atg7p, and Atg3p, and conjugated at the C terminus with phosphatidylethanolamine, to become the form essential for generation of autophagosomes
2760;UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate
2761;RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telomere position effect and telomere length
2762;Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
2763;Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3 and H4 genes; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
2764;Protein of the endoplasmic reticulum membrane involved in chitin deposition in the cell wall; overproduction confers resistance to Congo Red
2765;Plasma membrane multidrug transporter, member of the major facilitator superfamily; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs
2766;Hypothetical protein
2767;Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
2768;Protein anchored to the mitochondrial inner membrane, similar to Sco1p and may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p
2769;RNA polymerase I enhancer binding protein; DNA binding protein which binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription
2770;ORF, Dubious
2771;Hypothetical protein
2772;Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
2773;Hsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum
2774;Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance
2775;Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway
2776;Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
2777;TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
2778;Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase
2779;Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
2780;Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
2781;Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
2782;Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors
2783;Putative protein of unknown function; YBR184W is not an essential gene
2784;Protein of unknown function, has similarity to Erv14p
2785;RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; expressed in stationary phase
2786;Mitochondrial ATP-binding protein, possibly a mitochondrial chaperone with non-proteolytic function; similar to bacterial ClpX proteins
2787;Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
2788;Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase P
2789;Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR261C is not an essential gene
2790;The authentic, non-tagged protein was localized to the mitochondria
2791;3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family2792;Hypothetical protein
2793;UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p
2794;Third-largest subunit of DNA polymerase II (DNA polymerase epsilon), required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; mRNA abundance peaks at the G1/S boundary of the cell cycle
2795;RNA polymerase II-associated protein, defines a large complex that is biochemically and functionally distinct from the Srb-Mediator form of Pol II holoenzyme and is required for full expression of a subset of cell cycle-regulated genes2796;Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family
2797;Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p
2798;AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the putative tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
2799;Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall
2800;DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
【０１７３】
2801;Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
2802;Protein necessary for structural stability of L-A double-stranded RNA-containing particles
2803;Subunit of the RSC chromatin remodeling complex, a multisubunit complex that functions in transcriptional regulation, chromosome stability and establishing sister chromatid cohesion
2804;Hypothetical protein
2805;Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph
2806;Protein involved in rRNA processing; component of the exosome 3->5 exonucle
ase complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, 18S and 25S rRNA
2807;Putative ribokinase
2808;Low-affinity inorganic phosphate (Pi) transporter, involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments
2809;Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
2810;Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
2811;The authentic, non-tagged protein was localized to the mitochondria; cell cortex protein; not required for growth on nonfermentable carbon sources; required for viability in stationary phase
2812;Hypothetical protein
2813;Hypothetical protein
2814;Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
2815;Hypothetical protein
2816;Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p
2817;Hypothetical protein
2818;Protein of unknown function
2819;Bifunctional enzyme containing both alcohol dehydrogenase and glutathione-dependent formaldehyde dehydrogenase activities, functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p
2820;Hypothetical protein
2821;Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
2822;Hypothetical protein
2823;Cardiolipin synthase; produces cardiolipin, which is an important constituent of mitochondrial membranes; required for normal mitochondrial membrane potential and function
2824;Plasma membrane glucose receptor, highly similar to Snf3p; both Rgt2p and Snf3p serve as transmembrane glucose sensors generating an intracellular signal that induces expression of glucose transporter (HXT) genes
2825;Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis
2826;Protein of unknown function, deletion causes hypersensitivity to the K1 killer toxin
2827;Peripherally bound inner membrane protein of the mitochondrial matrix, required for export of C-terminal tail of Cox2p through the inner membrane
2828;Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
2829;Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site
2830;Pbp1p binding protein, interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system
2831;RNA binding protein required for maturation of tRNA and snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen
2832;Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant 2833;Subunit of the SF3a splicing factor complex, required for spliceosome assembly
2834;Mitochondrial tRNA pseudouridine synthase involved in pseudouridylation of mitochondrial tRNAs at position 32
2835;Ran GTpase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
2836;Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
2837;Transcriptional regulator involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p and Gal4p to relieve inhibition by Gal80p; binds galactose and ATP but does not have galactokinase activity
2838;Protein of unknown function; mutation confers radiation sensitivity
2839;T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
2840;Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis 
2841;Hypothetical protein
2842;Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
2843;Mitochondrial protein, member of the ATP-binding cassette (ABC) transporter family; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance
2844;Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
2845;Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
2846;Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport; potential Cdc28p substrate
2847;Actin-related protein
2848;Hypothetical protein
2849;Hypothetical protein
2850;Ubiquitin-protein ligase, involved in the proteasome-dependent degradation of aberrant nuclear proteins; san1 mutations suppress sir4, spt16, and cdc68 mutations, suggesting a role in chromatin silencing
2851;Ethanolamine kinase, primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; also exhibits choline kinase activity
2852;Nuclear protein, possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern
2853;RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes
2854;predicted to catalyze the final step in synthesis of Coenzyme A
2855;Hypothetical protein
2856;Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation
2857;U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
2858;Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p
2859;Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex (alpha, beta1, beta3) which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides
2860;Putative basic leucine zipper (bZIP) transcription factor; overexpression increases sodium and lithium tolerance
2861;Hypothetical protein
2862;Essential protein involved in assembly of cytosolic and nuclear iron-sulfur proteins
2863;Protein involved in rRNA processing; component of the exosome 3->5 exonucle
ase complex
2864;Protein of unknown function, expression is regulated by phosphate levels
2865;Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
2866;Hypothetical protein
2867;Putative DNA helicase
2868;Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
2869;Gamma-glutamyl kinase, catalyzes the first step in proline biosynthesis
2870;Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain
2871;Transcriptional repressor required for repression of middle sporulation-specific genes during mitosis; regulated by the pachytene checkpoint; a dominant mutation acts as a suppressor of silencing defects of SIR2 mutations
2872;Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
2873;Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation and for chromatin binding of the condensin complex
2874;Peroxisomal membrane protein (PMP) required to recruit Pex19p chaperone to peroxisomes; plays selective, essential, direct role in PMP import as a docking factor for Pex19p
2875;UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p
2876;Hypothetical protein
2877;Hypothetical protein
2878;One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
2879;Hypothetical protein
2880;Sporulation-specific chitinase
2881;GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
2882;Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the formation of both inosine monophosphate and guanosine monophosphate; mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
2883;N-formyltyrosine oxidase, sporulation-specific microsomal enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall, homologous to cytochrome P-450s
2884;Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane 
2885;Protein of unknown function, localizes to the ER, contains four transmembrane domains; member of the Der1p-like family
2886;Hypothetical protein
2887;DNA polymerase eta, involved in the predominantly error-free bypass replication of DNA lesions, catalyzes the efficient and accurate synthesis of DNA opposite cyclobutane pyrimidine dimers; homolog of human POLH and bacterial DinB proteins
2888;Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Sp homolog
2889;Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
2890;Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2
2891;Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
2892;Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2893;Arginine methyltransferase; ribosomal protein L12 is a substrate
2894;Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; has similarity to C. elegans Dpy-30
2895;One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
2896;Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of inorganic phosphate and by a system consisting of Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides
2897;Membrane protein involved in zinc metabolism, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
2898;The authentic, non-tagged protein was localized to the mitochondria
2899;Cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
2900;Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; forms a complex with the cytosolic Tip20p
【０１７４】
2901;Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes
2902;Plasma membrane arginine permease, requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance
2903;Protein with sequence similarity to the human MybBP1A and weak sequence similar to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
2904;Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family, localized to mitochondria
2905;Transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
2906;Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog
2907;Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
2908;RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p
2909;Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, may regulate transcription of genes involved in utilization of non-optimal carbon sources
2910;Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
2911;Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p
2912;Protein required for assembly of cytochrome c oxidase
2913;Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration
2914;Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
2915;5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a five related enzymes, which are active as heteromultimeric complexes
2916;Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle
2917;Hypothetical protein
2918;Protein that forms a heterodimeric histone-fold NC2 general transcription regulator complex with Ydr1p that binds to TBP and represses RNA pol II transcription during assembly of the preinitiation complex, homologous to human NC2alpha
2919;Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport
2920;5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis
2921;Protein of unknown function, nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin
2922;Protein of unknown function, nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin
2923;Putative protein of unknown function; YFL042C is not an essential gene
2924;Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; originally identified as a regulator of IME2
2925;F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
2926;Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in protein localization and non-autophagic vacuolar fusion2927;Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein
2928;Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles
2929;Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
2930;Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds
2931;Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk2p and mammalian Cam Kinase II
2932;Hypothetical protein
2933;Putative protein of unknown function; YFR039C is not an essential gene
2934;Cytosolic protein required for sporulation
2935;Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans
2936;Transcription factor of the Zn[II]2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression
2937;Oxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol)
2938;Hypothetical protein
2939;Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate
2940;Protein required for ubiquinone (coenzyme Q) biosynthesis and for respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis
2941;Protein involved in the initiation of DNA replication, required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p
2942;Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
2943;The authentic, non-tagged protein was localized to the mitochondria
2944;Hypothetical protein
2945;Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
2946;Probable 73 kDa subunit of SAGA histone acetyltransferase complex
2947;Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
2948;Identified by expression profiling and mass spectrometry
2949;Putative cysteine synthase; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
2950;Potential regulatory effector of CDC4 function, suppresses a temperature-sensitive allele of CDC4, tripartite protein structure in which a charged region separates two uncharged domains, not essential for mitosis or meiosis
【０１７５】
2951;One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; required for expression of mid-late sporulation-specific genes; contains two essential  bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
2952;Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
2953;Splicing factor, component of the U4/U6-U5 snRNP complex
2954;Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
2955;Separase with cysteine protease activity (related to caspases) that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibited by Pds1p
2956;Essential DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect2957;Essential protein that interacts with histones and is involved in nucleosome disassembly and reassembly during transcription elongation
2958;Hypothetical protein
2959;Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation
2960;Hypothetical protein
2961;Cytoplasmic GTP binding protein, negative regulator of the Ran/Tc4 GTPase cycle downstream of Gtr1p; homolog of human RagC and RagD proteins; component of the EGO complex, which is involved in the regulation of microautophagy
2962;Putative glycosidase of the cell wall, may have a role in cell wall architecture
2963;ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases
2964;Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions
2965;Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
2966;Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
2967;Putative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to both 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