【手続補正書】
【提出日】平成16年4月6日(2004.4.6)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０１２１
【補正方法】変更
【補正の内容】
【０１２１】
本出願を通して上に引用されたあらゆる参照文献は、参照として本明細書により完全に組み入れられている。
【配列表】
SEQUENCE LISTING
<110> The Government of the United States of America, as represented by the Secretary, Department of Health and Human Services
<120> Molecular Clones With Mutated HIV gag/pol, SIV gag and
SIV env Genes
<130> TO-A0324
<140> JP   2003-502211
<141> 2002-05-31
<150> US   09/872,733
<151> 2001-06-01
<160> 19                            
<170> PatentIn Ver. 2.1
<210> 1
<211> 4338
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:mutated HIV-1
gag/pol molecular clone
<400> 1
atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgatggga aaaaattcgg 60    
ttaaggccag ggggaaagaa gtacaagcta aagcacatcg tatgggcaag cagggagcta 120   
gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg 180   
ggacagctac aaccatccct tcagacagga tcagaggagc ttcgatcact atacaacaca 240   
gtagcaaccc tctattgtgt gcaccagcgg atcgagatca aggacaccaa ggaagcttta 300   
gacaagatag aggaagagca aaacaagtcc aagaagaagg cccagcaggc agcagctgac 360   
acaggacaca gcaatcaggt cagccaaaat taccctatag tgcagaacat ccaggggcaa 420   
atggtacatc aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag 480   
aaggctttca gcccagaagt gatacccatg ttttcagcat tatcagaagg agccacccca 540   
caggacctga acacgatgtt gaacaccgtg gggggacatc aagcagccat gcaaatgtta 600   
aaagagacca tcaatgagga agctgcagaa tgggatagag tgcatccagt gcatgcaggg 660   
cctattgcac caggccagat gagagaacca aggggaagtg acatagcagg aactactagt 720   
acccttcagg aacaaatagg atggatgaca aataatccac ctatcccagt aggagagatc 780   
tacaagaggt ggataatcct gggattgaac aagatcgtga ggatgtatag ccctaccagc 840   
attctggaca taagacaagg accaaaggaa ccctttagag actatgtaga ccggttctat 900   
aaaactctaa gagctgagca agcttcacag gaggtaaaaa attggatgac agaaaccttg 960   
ttggtccaaa atgcgaaccc agattgtaag accatcctga aggctctcgg cccagcggct 1020  
acactagaag aaatgatgac agcatgtcag ggagtaggag gacccggcca taaggcaaga 1080  
gttttggccg aggcgatgag ccaggtgacg aactcggcga ccataatgat gcagagaggc 1140  
aacttccgga accagcggaa gatcgtcaag tgcttcaatt gtggcaaaga agggcacacc 1200  
gccaggaact gccgggcccc ccggaagaag ggctgttgga aatgtggaaa ggaaggacac 1260  
caaatgaaag attgtactga gagacaggct aattttttag ggaagatctg gccttcctac 1320  
aagggaaggc cagggaattt tcttcagagc agaccagagc caacagcccc accagaagag 1380  
agcttcaggt ctggggtaga gacaacaact ccccctcaga agcaggagcc gatagacaag 1440  
gaactgtatc ctttaacttc cctcagatca ctctttggca acgacccctc gtcacagtaa 1500  
ggatcggggg gcaactcaag gaagcgctgc tcgatacagg agcagatgat acagtattag 1560  
aagaaatgag tttgccagga agatggaaac caaaaatgat aggggggatc gggggcttca 1620  
tcaaggtgag gcagtacgac cagatactca tagaaatctg tggacataaa gctataggta 1680  
cagtattagt aggacctacc tacacctgtc aacataattg gaagaaatct gttgacccag 1740  
atcggctgca ccttgaactt ccccatcagc cctattgaga cggtgcccgt gaagttgaag 1800  
ccggggatgg acggccccaa ggtcaagcaa tggccattga cgaaagagaa gatcaaggcc 1860  
ttagtcgaaa tctgtacaga gatggagaag gaagggaaga tcagcaagat cgggcctgag 1920  
aacccctaca acactccagt cttcgcaatc aagaagaagg acagtaccaa gtggagaaag 1980  
ctggtggact tcagagagct gaacaagaga actcaggact tctgggaagt tcagctgggc 2040  
atcccacatc ccgctgggtt gaagaagaag aagtcagtga cagtgctgga tgtgggtgat 2100  
gcctacttct ccgttccctt ggacgaggac ttcaggaagt acactgcctt cacgatacct 2160  
agcatcaaca acgagacacc aggcatccgc taccagtaca acgtgctgcc acagggatgg 2220  
aagggatcac cagccatctt tcaaagcagc atgaccaaga tcctggagcc cttccgcaag 2280  
caaaacccag acatcgtgat ctatcagtac atggacgacc tctacgtagg aagtgacctg 2340  
gagatcgggg cagcacagga ccaagatcga ggagctgaga cagcatctgt tgaggtgggg 2400  
actgaccaca ccagacaaga agcaccagaa ggaacctccc ttcctgtgga tgggctacga 2460  
actgcatcct gacaagtgga cagtgcagcc catcgtgctg cctgagaagg acagctggac 2520  
tgtgaacgac atacagaagc tcgtgggcaa gttgaactgg gcaagccaga tctacccagg 2580  
catcaaagtt aggcagctgt gcaagctgct tcgaggaacc aaggcactga cagaagtgat 2640  
cccactgaca gaggaagcag agctagaact ggcagagaac cgagagatcc tgaaggagcc 2700  
agtacatgga gtgtactacg acccaagcaa ggacctgatc gcagagatcc agaagcaggg 2760  
gcaaggccaa tggacctacc aaatctacca ggagcccttc aagaacctga agacaggcaa 2820  
gtacgcaagg atgaggggtg cccacaccaa cgatgtgaag cagctgacag aggcagtgca 2880  
gaagatcacc acagagagca tcgtgatctg gggcaagact cccaagttca agctgcccat 2940  
acagaaggag acatgggaga catggtggac cgagtactgg caagccacct ggatccctga 3000  
gtgggagttc gtgaacaccc ctcccttggt gaaactgtgg tatcagctgg agaaggaacc 3060  
catcgtggga gcagagacct tctacgtgga tggggcagcc aacagggaga ccaagctggg 3120  
caaggcaggc tacgtgacca accgaggacg acagaaagtg gtgaccctga ctgacaccac 3180  
caaccagaag actgagctgc aagccatcta cctagctctg caagacagcg gactggaagt 3240  
gaacatcgtg acagactcac agtacgcatg ggcatcatcc aagcacaacc agaccaatcc 3300  
gagtcagagc tggtgaacca gatcatcgag cagctgatca agaaggagaa agtgtacctg 3360  
gcatgggtac cagcacacaa aggaattgga ggaaatgaac aagtagataa attagtcagt 3420  
gctgggatcc ggaaggtgct gttcctggac gggatcgata aggcccaaga tgaacatgag 3480  
aagtaccact ccaactggcg cgctatggcc agcgacttca acctgccacc tgtagtagca 3540  
aaagaaatag tagccagctg tgataaatgt cagctaaaag gagaagccat gcatggacaa 3600  
gtagactgta gtccaggaat atggcagctg gactgcacgc acctggaggg gaaggtgatc 3660  
ctggtagcag ttcatgtagc cagtggatat atagaagcag aagttatccc tgctgaaact 3720  
gggcaggaaa cagcatattt tcttttaaaa ttagcaggaa gatggccagt aaaaacaata 3780  
cacacggaca acggaagcaa cttcactggt gctacggtta aggccgcctg ttggtgggcg 3840  
ggaatcaagc aggaatttgg aattccctac aatccccaat cgcaaggagt cgtggagagc 3900  
atgaacaagg agctgaagaa gatcatcgga cagtgaggga tcaggctgag cacctgaaga 3960  
cagcagtgca gatggcagtg ttcatccaca acttcaaaag aaaagggggg attggggggt 4020  
acagtgcagg ggaaaggatc gtggacatca tcgccaccga catccaaacc aaggagctgc 4080  
agaagcagat caccaagatc cagaacttcc gggtgtacta ccgcgacagc cgcaacccac 4140  
tgtggaaggg accagcaaag ctcctctgga agggagaggg ggcagtggtg atccaggaca 4200  
acagtgacat caaagtggtg ccaaggcgca aggccaagat catccgcgac tatggaaaac 4260  
agatggcagg tgatgattgt gtggcaagta gacaggatga ggattagaac ctggaagagc 4320  
ctggtgaagc accatatg                                               4338  
<210> 2
<211> 2507
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:wild-type pol
region in plasmid pCMVgagpolBNkan
<400> 2
tgtacagaga tggaaaagga agggaaaatt tcaaaaattg ggcctgaaaa tccatacaat 60    
actccagtat ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc 120   
agagaactta ataagagaac tcaagacttc tgggaagttc aattaggaat accacatccc 180   
gcagggttaa aaaagaaaaa atcagtaaca gtactggatg tgggtgatgc atatttttca 240   
gttcccttag atgaagactt caggaaatat actgcattta ccatacctag tataaacaat 300   
gagacaccag ggattagata ccatacctag tataaacaat gagacaccag ggatttgata 360   
tcagtacaat gtgcttccac agggatggaa aggatcacca gcaatattcc aaagtagcat 420   
gacaaaaatc ttagagcctt ttagaaaaca aaatccagac atagttatct atcaatacat 480   
ggatgatttg tatgtaggat ctgacttaga aatagggcag catagaacaa aaatagagga 540   
gctgagacaa catctgttga ggtggggact taccacacca gacaaaaaac atcagaaaga 600   
acctccattc ctttggatgg gttatgaact ccatcctgat aaatggacag tacagcctat 660   
agtgctgcca gaaaaagaca gctggactgt caatgacata cagaagttag tggggaaatt 720   
gaattgggca agtcagattt acccagggat taaagtaagg caattatgta aactccttag 780   
aggaaccaaa gcactaacag aagtaatacc actaacagaa gaagcagagc tagaactggc 840   
agaaaacaga gagattctaa aagaaccagt acatggagtg tattatgacc catcaaaaga 900   
cttaatagca gaaatacaga agcaggggca aggccaatgg acatatcaaa tttatcaaga 960   
gccatttaaa aatctgaaaa caggaaaata tgcaagaatg aggggtgccc acactaatga 1020  
tgtaaaacaa ttaacagagg cagtgcaaaa aataaccaca gaaagcatag taatatgggg 1080  
aaagactcct aaatttaaac tgcccataca aaaggaaaca tgggaaacat ggtggacaga 1140  
gtattggcaa gccacctgga ttcctgagtg ggagtttgtt aatacccctc ctttagtgaa 1200  
attatggtac cagttagaga aagaacccat agtaggagca gaaaccttct atgtagatgg 1260  
ggcagctaac agggagacta aattaggaaa agcaggatat gttactaata gaggaagaca 1320  
aaaagttgtc accctaactg acacaacaaa tcagaagact gagttacaag caatttatct 1380  
agctttgcag gattcgggat tagaagtaaa catagtaaca gactcacaat atgcattagg 1440  
aatcattcaa gcacaaccag atcaaagtga atcagagtta gtcaatcaaa taatagagca 1500  
gttaataaaa aaggaaaagg tctatctggc atgggtacca gcacacaaag gaattggagg 1560  
aaatgaacaa gtagataaat tagtcagtgc tggaatcagg aaagtactat ttttagatgg 1620  
aatagataag gcccaagatg aacatgagaa atatcacagt aattggagag caatggctag 1680  
tgattttaac ctgccacctg tagtagcaaa agaaatagta gccagctgtg ataaatgtca 1740  
gctaaaagga gaagccatgc atggacaagt agactgtagt ccaggaatat ggcaactaga 1800  
ttgtacacat ttagaaggaa aagttatcct ggtagcagtt catgtagcca gtggatatat 1860  
agaagcagaa gttattccag cagaaacagg gcaggaaaca gcatattttc ttttaaaatt 1920  
agcaggaaga tggccagtaa aaacaataca tacagacaat ggcagcaatt tcaccagtgc 1980  
tacggttaag gccgcctgtt ggtgggcggg aatcaagcag gaatttggaa ttccctacaa 2040  
tccccaaagt caaggagtag tagaatctat gaataaagaa ttaaagaaaa ttataggaca 2100  
ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat tcatccacaa 2160  
ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 2220  
agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg 2280  
ggtttattac agggacagca gaaatccact ttggaaagga ccagcaaagc tcctctggaa 2340  
aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc caagaagaaa 2400  
agcaaagatc attagggatt atggaaaaca gatggcaggt gatgattgtg tggcaagtag 2460  
acaggatgag gattagaaca tggaaaagtt tagtaaaaca ccatatg               2507  
<210> 3
<211> 2467
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:mutated pol
region in plasmid pCMVgagpolBNkan
<400> 3
tgtacagaga tggagaagga agggaagatc agcaagatcg ggcctgagaa cccctacaac 60    
actccagtct tcgcaatcaa gaagaaggac agtaccaagt ggagaaagct ggtggacttc 120   
agagagctga acaagagaac tcaggacttc tgggaagttc agctgggcat cccacatccc 180   
gctgggttga agaagaagaa gtcagtgaca gtgctggatg tgggtgatgc ctacttctcc 240   
gttcccttgg acgaggactt caggaagtac actgccttca cgatacctag catcaacaac 300   
gagacaccag gcatccgcta ccagtacaac gtgctgccac agggatggaa gggatcacca 360   
gccatctttc aaagcagcat gaccaagatc ctggagccct tccgcaagca aaacccagac 420   
atcgtgatct atcagtacat ggacgacctc tacgtaggaa gtgacctgga gatcgggcag 480   
cacaggacca agatcgagga gctgagacag catctgttga ggtggggact gaccacacca 540   
gacaagaagc accagaagga acctcccttc ctgtggatgg gctacgaact gcatcctgac 600   
aagtggacag tgcagcccat cgtgctgcct gagaaggaca gctggactgt gaacgacata 660   
cagaagctcg tgggcaagtt gaactgggca agccagatct acccaggcat caaagttagg 720   
cagctgtgca agctgcttcg aggaaccaag gcactgacag aagtgatccc actgacagag 780   
gaagcagagc tagaactggc agagaaccga gagatcctga aggagccagt acatggagtg 840   
tactacgacc caagcaagga cctgatcgca gagatccaga agcaggggca aggccaatgg 900   
acctaccaaa tctaccagga gcccttcaag aacctgaaga caggcaagta cgcaaggatg 960   
aggggtgccc acaccaacga tgtgaagcag ctgacagagg cagtgcagaa gatcaccaca 1020  
gagagcatcg tgatctgggg caagactccc aagttcaagc tgcccataca gaaggagaca 1080  
tgggagacat ggtggaccga gtactggcaa gccacctgga tccctgagtg ggagttcgtg 1140  
aacacccctc ccttggtgaa actgtggtat cagctggaga aggaacccat cgtgggagca 1200  
gagaccttct acgtggatgg ggcagccaac agggagacca agctgggcaa ggcaggctac 1260  
gtgaccaacc gaggacgaca gaaagtggtg accctgactg acaccaccaa ccagaagact 1320  
gagctgcaag ccatctacct agctctgcaa gacagcggac tggaagtgaa catcgtgaca 1380  
gactcacagt acgcactggg catcatccaa gcacaaccag accaatccga gtcagagctg 1440  
gtgaaccaga tcatcgagca gctgatcaag aaggagaaag tgtacctggc atgggtacca 1500  
gcacacaaag gaattggagg aaatgaacaa gtagataaat tagtcagtgc tgggatccgg 1560  
aaggtgctgt tcctggacgg gatcgataag gcccaagatg aacatgagaa gtaccactcc 1620  
aactggcgcg ctatggccag cgacttcaac ctgccacctg tagtagcaaa agaaatagta 1680  
gccagctgtg ataaatgtca gctaaaagga gaagccatgc atggacaagt agactgtagt 1740  
ccaggaatat ggcagctgga ctgcacgcac ctggagggga aggtgatcct ggtagcagtt 1800  
catgtagcca gtggatatat agaagcagaa gttatccctg ctgaaactgg gcaggaaaca 1860  
gcatattttc ttttaaaatt agcaggaaga tggccagtaa aaacaataca cacggacaac 1920  
ggaagcaact tcactggtgc tacggttaag gccgcctgtt ggtgggcggg aatcaagcag 1980  
gaatttggaa ttccctacaa tccccaatcg caaggagtcg tggagagcat gaacaaggag 2040  
ctgaagaaga tcatcggaca agtgagggat caggctgagc acctgaagac agcagtgcag 2100  
atggcagtgt tcatccacaa cttcaaaaga aaagggggga ttggggggta cagtgcaggg 2160  
gaaaggatcg tggacatcat cgccaccgac atccaaacca aggagctgca gaagcagatc 2220  
accaagatcc agaacttccg ggtgtactac cgcgacagcc gcaacccact gtggaaggga 2280  
ccagcaaagc tcctctggaa gggagagggg gcagtggtga tccaggacaa cagtgacatc 2340  
aaagtggtgc caaggcgcaa ggccaagatc atccgcgact atggaaaaca gatggcaggt 2400  
gatgattgtg tggcaagtag acaggatgag gattagaacc tggaagagcc tggtgaagca 2460  
ccatatg                                                           2467  
<210> 4
<211> 1533
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:mutated
Rev-independent SIV gag molecular clone SIVgagDX
<400> 4
atgggcgtga gaaactccgt cttgtcaggg aagaaagcag atgaattaga aaaaattagg 60    
ctacgaccca acggaaagaa aaagtacatg ttgaagcatg tagtatgggc agcaaatgaa 120   
ttagatagat ttggattagc agaaagcctg ttggagaaca aagaaggatg tcaaaaaata 180   
ctttcggtct tagctccatt agtgccaaca ggctcagaaa atttaaaaag cctttataat 240   
actgtctgcg tcatctggtg cattcacgca gaagagaaag tgaaacacac tgaggaagca 300   
aaacagatag tgcagagaca cctagtggtg gaaacaggaa ccaccgaaac catgccgaag 360   
acctctcgac caacagcacc atctagcggc agaggaggaa actacccagt acagcagatc 420   
ggtggtaact acgtccacct gccactgtcc ccgagaaccc tgaacgcttg ggtcaagctg 480   
atcgaggaga agaagttcgg agcagaagta gtgccaggat tccaggcact gtcagaaggt 540   
tgcaccccct acgacatcaa ccagatgctg aactgcgttg gagaccatca ggcggctatg 600   
cagatcatcc gtgacatcat caacgaggag gctgcagatt gggacttgca gcacccacaa 660   
ccagctccac aacaaggaca acttagggag ccgtcaggat cagacatcgc aggaaccacc 720   
tcctcagttg acgaacagat ccagtggatg taccgtcagc agaacccgat cccagtaggc 780   
aacatctacc gtcgatggat ccagctgggt ctgcagaagt gcgtccgtat gtacaacccg 840   
accaacattc tagatgtaaa acaagggcca aaagagccat ttcagagcta tgtagacagg 900   
ttctacaaaa gtttaagagc agaacagaca gatgcagcag taaagaattg gatgactcaa 960   
acactgctga ttcaaaatgc taacccagat tgcaagctag tgctgaaggg gctgggtgtg 1020  
aatcccaccc tagaagaaat gctgacggct tgtcaaggag taggggggcc gggacagaag 1080  
gctagattaa tggcagaagc cctgaaagag gccctcgcac cagtgccaat cccttttgca 1140  
gcagcccaac agaggggacc aagaaagcca attaagtgtt ggaattgtgg gaaagaggga 1200  
cactctgcaa ggcaatgcag agccccaaga agacagggat gctggaaatg tggaaaaatg 1260  
gaccatgtta tggccaaatg cccagacaga caggcgggtt ttttaggcct tggtccatgg 1320  
ggaaagaagc cccgcaattt ccccatggct caagtgcatc aggggctgat gccaactgct 1380  
cccccagagg acccagctgt ggatctgcta aagaactaca tgcagttggg caagcagcag 1440  
agagaaaagc agagagaaag cagagagaag ccttacaagg aggtgacaga ggatttgctg 1500  
cacctcaatt ctctctttgg aggagaccag tag                              1533  
<210> 5
<211> 1533
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:consensus
sequence of mutated SIVgagDX and wild type
Simian (macaque) immunodeficiency virus
isolate 239, clone lambda siv 239-1 in Fig. 4
<400> 5
atgggcgtga gaaactccgt cttgtcaggg aagaaagcag atgaattaga aaaaattagg 60    
ctacgaccca acggaaagaa aaagtacatg ttgaagcatg tagtatgggc agcaaatgaa 120   
ttagatagat ttggattagc agaaagcctg ttggagaaca aagaaggatg tcaaaaaata 180   
ctttcggtct tagctccatt agtgccaaca ggctcagaaa atttaaaaag cctttataat 240   
actgtctgcg tcatctggtg cattcacgca gaagagaaag tgaaacacac tgaggaagca 300   
aaacagatag tgcagagaca cctagtggtg gaaacaggaa cmacmgaaac yatgccraar 360   
acmwstmgac caacagcacc atctagcggc agaggaggaa aytacccagt acarcaratm 420   
ggtggtaact aygtccacct gccaytrwsc ccgagaacmy traaygcytg ggtmaarytg 480   
atmgaggara agaarttygg agcagaagta gtgccaggat tycaggcact gtcagaaggt 540   
tgcaccccct aygacatyaa ycagatgytr aaytgygtkg gagaccatca rgcggctatg 600   
cagatyatcm gwgayatyat maacgaggag gctgcagatt gggacttgca gcacccacaa 660   
ccagctccac aacaaggaca acttagggag ccgtcaggat cagayatygc aggaacmacy 720   
wsytcagtwg aygaacarat ccagtggatg tacmgwcarc agaacccsat mccagtaggc 780   
aacatytacm gkmgatggat ccarctgggk ytgcaraart gygtymgwat gtayaacccr 840   
acmaacattc tagatgtaaa acaagggcca aaagagccat ttcagagcta tgtagacagg 900   
ttctacaaaa gtttaagagc agaacagaca gatgcagcag taaagaattg gatgactcaa 960   
acactgctga ttcaaaatgc taacccagat tgcaagctag tgctgaaggg gctgggtgtg 1020  
aatcccaccc tagaagaaat gctgacggct tgtcaaggag taggggggcc gggacagaag 1080  
gctagattaa tggcagaagc cctgaaagag gccctcgcac cagtgccaat cccttttgca 1140  
gcagcccaac agaggggacc aagaaagcca attaagtgtt ggaattgtgg gaaagaggga 1200  
cactctgcaa ggcaatgcag agccccaaga agacagggat gctggaaatg tggaaaaatg 1260  
gaccatgtta tggccaaatg cccagacaga caggcgggtt ttttaggcct tggtccatgg 1320  
ggaaagaagc cccgcaattt ccccatggct caagtgcatc aggggctgat gccaactgct 1380  
cccccagagg acccagctgt ggatctgcta aagaactaca tgcagttggg caagcagcag 1440  
agagaaaagc agagagaaag cagagagaag ccttacaagg aggtgacaga ggatttgctg 1500  
cacctcaatt ctctctttgg aggagaccag tag                              1533 
<210> 6
<211> 8366
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:packaging
construct pCMVgagpolBNkan
<400> 6
cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60    
caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120   
ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180   
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240   
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300   
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360   
acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420   
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480   
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540   
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600   
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660   
ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720   
gaagacaccg ggaccgatcc agcctccgcg ggcgcgcgtc gacagagaga tgggtgcgag 780   
agcgtcagta ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg 840   
gggaaagaag aagtacaagc taaagcacat cgtatgggca agcagggagc tagaacgatt 900   
cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct 960   
acaaccatcc cttcagacag gatcagagga gcttcgatca ctatacaaca cagtagcaac 1020  
cctctattgt gtgcaccagc ggatcgagat caaggacacc aaggaagctt tagacaagat 1080  
agaggaagag caaaacaagt ccaagaagaa ggcccagcag gcagcagctg acacaggaca 1140  
cagcaatcag gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca 1200  
tcaggccata tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt 1260  
cagcccagaa gtgataccca tgttttcagc attatcagaa ggagccaccc cacaggacct 1320  
gaacacgatg ttgaacaccg tggggggaca tcaagcagcc atgcaaatgt taaaagagac 1380  
catcaatgag gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc 1440  
accaggccag atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca 1500  
ggaacaaata ggatggatga caaataatcc acctatccca gtaggagaga tctacaagag 1560  
gtggataatc ctgggattga acaagatcgt gaggatgtat agccctacca gcattctgga 1620  
cataagacaa ggaccaaagg aaccctttag agactatgta gaccggttct ataaaactct 1680  
aagagctgag caagcttcac aggaggtaaa aaattggatg acagaaacct tgttggtcca 1740  
aaatgcgaac ccagattgta agaccatcct gaaggctctc ggcccagcgg ctacactaga 1800  
agaaatgatg acagcatgtc agggagtagg aggacccggc cataaggcaa gagttttggc 1860  
cgaggcgatg agccaggtga cgaactcggc gaccataatg atgcagagag gcaacttccg 1920  
gaaccagcgg aagatcgtca agtgcttcaa ttgtggcaaa gaagggcaca ccgccaggaa 1980  
ctgccgggcc ccccggaaga agggctgttg gaaatgtgga aaggaaggac accaaatgaa 2040  
agattgtact gagagacagg ctaatttttt agggaagatc tggccttcct acaagggaag 2100  
gccagggaat tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag 2160  
gtctggggta gagacaacaa ctccccctca gaagcaggag ccgatagaca aggaactgta 2220  
tcctttaact tccctcagat cactctttgg caacgacccc tcgtcacagt aaggatcggg 2280  
gggcaactca aggaagcgct gctcgataca ggagcagatg atacagtatt agaagaaatg 2340  
agtttgccag gaagatggaa accaaaaatg atagggggga tcgggggctt catcaaggtg 2400  
aggcagtacg accagatact catagaaatc tgtggacata aagctatagg tacagtatta 2460  
gtaggaccta cacctgtcaa cataattgga agaaatctgt tgacccagat cggctgcacc 2520  
ttgaacttcc ccatcagccc tattgagacg gtgcccgtga agttgaagcc ggggatggac 2580  
ggccccaagg tcaagcaatg gccattgacg aaagagaaga tcaaggcctt agtcgaaatc 2640  
tgtacagaga tggagaagga agggaagatc agcaagatcg ggcctgagaa cccctacaac 2700  
actccagtct tcgcaatcaa gaagaaggac agtaccaagt ggagaaagct ggtggacttc 2760  
agagagctga acaagagaac tcaggacttc tgggaagttc agctgggcat cccacatccc 2820  
gctgggttga agaagaagaa gtcagtgaca gtgctggatg tgggtgatgc ctacttctcc 2880  
gttcccttgg acgaggactt caggaagtac actgccttca cgatacctag catcaacaac 2940  
gagacaccag gcatccgcta ccagtacaac gtgctgccac agggatggaa gggatcacca 3000  
gccatctttc aaagcagcat gaccaagatc ctggagccct tccgcaagca aaacccagac 3060  
atcgtgatct atcagtacat ggacgacctc tacgtaggaa gtgacctgga gatcgggcag 3120  
cacaggacca agatcgagga gctgagacag catctgttga ggtggggact gaccacacca 3180  
gacaagaagc accagaagga acctcccttc ctgtggatgg gctacgaact gcatcctgac 3240  
aagtggacag tgcagcccat cgtgctgcct gagaaggaca gctggactgt gaacgacata 3300  
cagaagctcg tgggcaagtt gaactgggca agccagatct acccaggcat caaagttagg 3360  
cagctgtgca agctgcttcg aggaaccaag gcactgacag aagtgatccc actgacagag 3420  
gaagcagagc tagaactggc agagaaccga gagatcctga aggagccagt acatggagtg 3480  
tactacgacc caagcaagga cctgatcgca gagatccaga agcaggggca aggccaatgg 3540  
acctaccaaa tctaccagga gcccttcaag aacctgaaga caggcaagta cgcaaggatg 3600  
aggggtgccc acaccaacga tgtgaagcag ctgacagagg cagtgcagaa gatcaccaca 3660  
gagagcatcg tgatctgggg caagactccc aagttcaagc tgcccataca gaaggagaca 3720  
tgggagacat ggtggaccga gtactggcaa gccacctgga tccctgagtg ggagttcgtg 3780  
aacacccctc ccttggtgaa actgtggtat cagctggaga aggaacccat cgtgggagca 3840  
gagaccttct acgtggatgg ggcagccaac agggagacca agctgggcaa ggcaggctac 3900  
gtgaccaacc gaggacgaca gaaagtggtg accctgactg acaccaccaa ccagaagact 3960  
gagctgcaag ccatctacct agctctgcaa gacagcggac tggaagtgaa catcgtgaca 4020  
gactcacagt acgcactggg catcatccaa gcacaaccag accaatccga gtcagagctg 4080  
gtgaaccaga tcatcgagca gctgatcaag aaggagaaag tgtacctggc atgggtacca 4140  
gcacacaaag gaattggagg aaatgaacaa gtagataaat tagtcagtgc tgggatccgg 4200  
aaggtgctgt tcctggacgg gatcgataag gcccaagatg aacatgagaa gtaccactcc 4260  
aactggcgcg ctatggccag cgacttcaac ctgccacctg tagtagcaaa agaaatagta 4320  
gccagctgtg ataaatgtca gctaaaagga gaagccatgc atggacaagt agactgtagt 4380  
ccaggaatat ggcagctgga ctgcacgcac ctggagggga aggtgatcct ggtagcagtt 4440  
catgtagcca gtggatatat agaagcagaa gttatccctg ctgaaactgg gcaggaaaca 4500  
gcatattttc ttttaaaatt agcaggaaga tggccagtaa aaacaataca cacggacaac 4560  
ggaagcaact tcactggtgc tacggttaag gccgcctgtt ggtgggcggg aatcaagcag 4620  
gaatttggaa ttccctacaa tccccaatcg caaggagtcg tggagagcat gaacaaggag 4680  
ctgaagaaga tcatcggaca agtgagggat caggctgagc acctgaagac agcagtgcag 4740  
atggcagtgt tcatccacaa cttcaaaaga aaagggggga ttggggggta cagtgcaggg 4800  
gaaaggatcg tggacatcat cgccaccgac atccaaacca aggagctgca gaagcagatc 4860  
accaagatcc agaacttccg ggtgtactac cgcgacagcc gcaacccact gtggaaggga 4920  
ccagcaaagc tcctctggaa gggagagggg gcagtggtga tccaggacaa cagtgacatc 4980  
aaagtggtgc caaggcgcaa ggccaagatc atccgcgact atggaaaaca gatggcaggt 5040  
gatgattgtg tggcaagtag acaggatgag gattagaacc tggaagagcc tggtgaagca 5100  
ccatatggcg ttcgaagcta gcctcgagat ccagatctgc tgtgccttct agttgccagc 5160  
catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 5220  
tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 5280  
tggggggtgg ggtggggcag cacagcaagg gggaggattg ggaagacaat agcaggcatg 5340  
ctggggatgc ggtgggctct atgggtaccc aggtgctgaa gaattgaccc ggttcctcct 5400  
gggccagaaa gaagcaggca catccccttc tctgtgacac accctgtcca cgcccctggt 5460  
tcttagttcc agccccactc ataggacact catagctcag gagggctccg ccttcaatcc 5520  
cacccgctaa agtacttgga gcggtctctc cctccctcat cagcccacca aaccaaacct 5580  
agcctccaag agtgggaaga aattaaagca agataggcta ttaagtgcag agggagagaa 5640  
aatgcctcca acatgtgagg aagtaatgag agaaatcata gaatttcttc cgcttcctcg 5700  
ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5760  
gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5820  
ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5880  
cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5940  
ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 6000  
accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 6060  
caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 6120  
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6180  
tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6240  
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6300  
actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6360  
gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 6420  
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6480  
gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6540  
aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6600  
atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6660  
gcgatctgtc tatttcgttc atccatagtt gcctgactcc gggggggggg ggcgctgagg 6720  
tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag 6780  
ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat 6840  
tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc 6900  
cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta 6960  
atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc 7020  
aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt 7080  
ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat 7140  
cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa 7200  
ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa 7260  
agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa 7320  
tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg 7380  
cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact 7440  
gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct 7500  
gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc 7560  
ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta 7620  
acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc 7680  
ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac 7740  
ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt 7800  
tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt 7860  
catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg 7920  
ctttcccccc ccccccatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 7980  
atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 8040  
gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 8100  
atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 8160  
cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 8220  
cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 8280  
cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 8340  
aaataccgca tcagattggc tattgg                                      8366  
<210> 7
<211> 271
<212> PRT
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:translation of
complementary strand positions 7814-7002 of
packaging construct pCMVgagpolBNkan (SEQ ID NO:6)
<400> 7
Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn  
1               5                  10                  15    
Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn  
20                  25                  30        
Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp  
35                  40                  45            
Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp  
50                  55                  60                
Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro  
65                  70                  75                  80
Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu  
85                  90                  95    
Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu  
100                 105                 110        
Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu  
115                 120                 125            
Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp  
130                 135                 140                
Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu  
145                 150                 155                 160
Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu  
165                 170                 175    
Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser  
180                 185                 190        
Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu  
195                 200                 205            
Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp  
210                 215                 220                
Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser  
225                 230                 235                 240
Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro  
245                 250                 255    
Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe      
260                 265                 270    
<210> 8
<211> 8937
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:transfer
construct 1, pmBCwCNluci
<400> 8
tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca 60    
cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac 120   
tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca 180   
aataaggaga gaagaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg 240   
agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag 300   
agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg 360   
ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat 420   
gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 480   
gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 540   
tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 600   
agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 660   
cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 720   
caagaggcga ggggcggcgc ctgacgagga cgccaaaaat tttgactagc ggaggctaga 780   
aggagagagc tcggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgatgggaa 840   
aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca 900   
agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt 960   
agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca 1020  
ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc 1080  
aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa 1140  
gcagcagctg acacaggaca cagcaatcag gtcagccaaa attaccctat agtgcagaac 1200  
atccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaacga taagcttggg 1260  
agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 1320  
gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 1380  
gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 1440  
atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 1500  
cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 1560  
ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 1620  
cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 1680  
atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 1740  
ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct 1800  
ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag aggatccatc 1860  
taagtaagct tggcattccg gtactgttgg taaaatggaa gacgccaaaa acataaagaa 1920  
aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc 1980  
tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt 2040  
gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg 2100  
atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt 2160  
tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta 2220  
taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc 2280  
caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat 2340  
tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac 2400  
atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga 2460  
caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc 2520  
ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa 2580  
tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat 2640  
gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga 2700  
agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc 2760  
aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt 2820  
acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa 2880  
acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta catcagctat 2940  
tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt 3000  
tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga 3060  
attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa 3120  
cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga 3180  
cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt 3240  
ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt 3300  
ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca 3360  
cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc 3420  
gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa 3480  
actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa 3540  
attgtaactc gagggggggc ccggtacctt taagaccaat gacttacaag gcagctgtag 3600  
atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa 3660  
gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga 3720  
actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag 3780  
taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac 3840  
accctgtgag cctgcatgga atggatgacc ctgagagaga agtgttagag tggaggtttg 3900  
acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact 3960  
gctgacatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc 4020  
tgggcgggac tggggagtgg cgagccctca gatgctgcat ataagcagct gctttttgcc 4080  
tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 4140  
aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 4200  
ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 4260  
tctagcaccc cccaggaggt agaggttgca gtgagccaag atcgcgccac tgcattccag 4320  
cctgggcaag aaaacaagac tgtctaaaat aataataata agttaagggt attaaatata 4380  
tttatacatg gaggtcataa aaatatatat atttgggctg ggcgcagtgg ctcacacctg 4440  
cgcccggccc tttgggaggc cgaggcaggt ggatcacctg agtttgggag ttccagacca 4500  
gcctgaccaa catggagaaa ccccttctct gtgtattttt agtagatttt attttatgtg 4560  
tattttattc acaggtattt ctggaaaact gaaactgttt ttcctctact ctgataccac 4620  
aagaatcatc agcacagagg aagacttctg tgatcaaatg tggtgggaga gggaggtttt 4680  
caccagcaca tgagcagtca gttctgccgc agactcggcg ggtgtccttc ggttcagttc 4740  
caacaccgcc tgcctggaga gaggtcagac cacagggtga gggctcagtc cccaagacat 4800  
aaacacccaa gacataaaca cccaacaggt ccaccccgcc tgctgcccag gcagagccga 4860  
ttcaccaaga cgggaattag gatagagaaa gagtaagtca cacagagccg gctgtgcggg 4920  
agaacggagt tctattatga ctcaaatcag tctccccaag cattcgggga tcagagtttt 4980  
taaggataac ttagtgtgta gggggccagt gagttggaga tgaaagcgta gggagtcgaa 5040  
ggtgtccttt tgcgccgagt cagttcctgg gtgggggcca caagatcgga tgagccagtt 5100  
tatcaatccg ggggtgccag ctgatccatg gagtgcaggg tctgcaaaat atctcaagca 5160  
ctgattgatc ttaggtttta caatagtgat gttaccccag gaacaatttg gggaaggtca 5220  
gaatcttgta gcctgtagct gcatgactcc taaaccataa tttctttttt gttttttttt 5280  
ttttattttt gagacagggt ctcactctgt cacctaggct ggagtgcagt ggtgcaatca 5340  
cagctcactg cagcccctag agcggccgcc accgcggtgg agctccaatt cgccctatag 5400  
tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 5460  
cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 5520  
agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga 5580  
aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5640  
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5700  
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 5760  
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 5820  
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 5880  
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 5940  
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 6000  
acccgccgcg cttaatgcgc cgctacaggg cgcgtcccag gtggcacttt tcggggaaat 6060  
gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 6120  
agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 6180  
catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 6240  
ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 6300  
atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 6360  
ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 6420  
gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 6480  
ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 6540  
ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 6600  
gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 6660  
ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 6720  
gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 6780  
ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 6840  
gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 6900  
gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 6960  
caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 7020  
cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 7080  
ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 7140  
taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 7200  
tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 7260  
gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 7320  
agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 7380  
aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 7440  
gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 7500  
gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 7560  
tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 7620  
agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 7680  
cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 7740  
gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 7800  
gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 7860  
ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 7920  
cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 7980  
cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 8040  
cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 8100  
gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 8160  
taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg aattaaccct 8220  
cactaaaggg aacaaaagct gctgcagggt ccctaactgc caagccccac agtgtgccct 8280  
gaggctgccc cttccttcta gcggctgccc ccactcggct ttgctttccc tagtttcagt 8340  
tacttgcgtt cagccaaggt ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga 8400  
aagagaccag ctttacaggg ggtttatcac agtgcaccct gacagtcgtc agcctcacag 8460  
ggggtttatc acattgcacc ctgacagtcg tcagcctcac agggggttta tcacagtgca 8520  
cccttacaat cattccattt gattcacaat ttttttagtc tctactgtgc ctaacttgta 8580  
agttaaattt gatcagaggt gtgttcccag aggggaaaac agtatataca gggttcagta 8640  
ctatcgcatt tcaggcctcc acctgggtct tggaatgtgt cccccgaggg gtgatgacta 8700  
cctcagttgg atctccacag gtcacagtga cacaagataa ccaagacacc tcccaaggct 8760  
accacaatgg gccgccctcc acgtgcacat ggccggagga actgccatgt cggaggtgca 8820  
agcacacctg cgcatcagag tccttggtgt ggagggaggg accagcgcag cttccagcca 8880  
tccacctgat gaacagaacc tagggaaagc cccagttcta cttacaccag gaaaggc    8937  
<210> 9
<211> 8937
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:transfer
construct 2, pmBCmCNluci
<400> 9
tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca 60    
cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac 120   
tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca 180   
aataaggaga gaagaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg 240   
agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag 300   
agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg 360   
ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat 420   
gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 480   
gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 540   
tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 600   
agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 660   
cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 720   
caagaggcga ggggcggcgc ctgacgagga cgccaaaaat tttgactagc ggaggctaga 780   
aggagagagc tcggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgatgggaa 840   
aaaattcggt taaggccagg gggaaagaag aagtacaagc taaagcacat cgtatgggca 900   
agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt 960   
agacaaatac tgggacagct acaaccatcc cttcagacag gatcagagga gcttcgatca 1020  
ctatacaaca cagtagcaac cctctattgt gtgcaccagc ggatcgagat caaggacacc 1080  
aaggaagctt tagacaagat agaggaagag caaaacaagt ccaagaagaa ggcccagcag 1140  
gcagcagctg acacaggaca cagcaatcag gtcagccaaa attaccctat agtgcagaac 1200  
atccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaacga taagcttggg 1260  
agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 1320  
gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 1380  
gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 1440  
atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 1500  
cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 1560  
ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 1620  
cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 1680  
atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 1740  
ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct 1800  
ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag aggatccatc 1860  
taagtaagct tggcattccg gtactgttgg taaaatggaa gacgccaaaa acataaagaa 1920  
aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc 1980  
tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt 2040  
gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg 2100  
atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt 2160  
tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta 2220  
taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc 2280  
caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat 2340  
tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac 2400  
atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga 2460  
caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc 2520  
ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa 2580  
tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat 2640  
gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga 2700  
agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc 2760  
aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt 2820  
acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa 2880  
acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta catcagctat 2940  
tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt 3000  
tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga 3060  
attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa 3120  
cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga 3180  
cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt 3240  
ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt 3300  
ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca 3360  
cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc 3420  
gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa 3480  
actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa 3540  
attgtaactc gagggggggc ccggtacctt taagaccaat gacttacaag gcagctgtag 3600  
atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa 3660  
gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga 3720  
actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag 3780  
taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac 3840  
accctgtgag cctgcatgga atggatgacc ctgagagaga agtgttagag tggaggtttg 3900  
acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact 3960  
gctgacatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc 4020  
tgggcgggac tggggagtgg cgagccctca gatgctgcat ataagcagct gctttttgcc 4080  
tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 4140  
aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 4200  
ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 4260  
tctagcaccc cccaggaggt agaggttgca gtgagccaag atcgcgccac tgcattccag 4320  
cctgggcaag aaaacaagac tgtctaaaat aataataata agttaagggt attaaatata 4380  
tttatacatg gaggtcataa aaatatatat atttgggctg ggcgcagtgg ctcacacctg 4440  
cgcccggccc tttgggaggc cgaggcaggt ggatcacctg agtttgggag ttccagacca 4500  
gcctgaccaa catggagaaa ccccttctct gtgtattttt agtagatttt attttatgtg 4560  
tattttattc acaggtattt ctggaaaact gaaactgttt ttcctctact ctgataccac 4620  
aagaatcatc agcacagagg aagacttctg tgatcaaatg tggtgggaga gggaggtttt 4680  
caccagcaca tgagcagtca gttctgccgc agactcggcg ggtgtccttc ggttcagttc 4740  
caacaccgcc tgcctggaga gaggtcagac cacagggtga gggctcagtc cccaagacat 4800  
aaacacccaa gacataaaca cccaacaggt ccaccccgcc tgctgcccag gcagagccga 4860  
ttcaccaaga cgggaattag gatagagaaa gagtaagtca cacagagccg gctgtgcggg 4920  
agaacggagt tctattatga ctcaaatcag tctccccaag cattcgggga tcagagtttt 4980  
taaggataac ttagtgtgta gggggccagt gagttggaga tgaaagcgta gggagtcgaa 5040  
ggtgtccttt tgcgccgagt cagttcctgg gtgggggcca caagatcgga tgagccagtt 5100  
tatcaatccg ggggtgccag ctgatccatg gagtgcaggg tctgcaaaat atctcaagca 5160  
ctgattgatc ttaggtttta caatagtgat gttaccccag gaacaatttg gggaaggtca 5220  
gaatcttgta gcctgtagct gcatgactcc taaaccataa tttctttttt gttttttttt 5280  
ttttattttt gagacagggt ctcactctgt cacctaggct ggagtgcagt ggtgcaatca 5340  
cagctcactg cagcccctag agcggccgcc accgcggtgg agctccaatt cgccctatag 5400  
tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 5460  
cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 5520  
agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga 5580  
aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5640  
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5700  
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 5760  
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 5820  
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 5880  
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 5940  
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 6000  
acccgccgcg cttaatgcgc cgctacaggg cgcgtcccag gtggcacttt tcggggaaat 6060  
gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 6120  
agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 6180  
catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 6240  
ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 6300  
atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 6360  
ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 6420  
gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 6480  
ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 6540  
ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 6600  
gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 6660  
ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 6720  
gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 6780  
ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 6840  
gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 6900  
gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 6960  
caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 7020  
cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 7080  
ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 7140  
taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 7200  
tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 7260  
gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 7320  
agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 7380  
aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 7440  
gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 7500  
gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 7560  
tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 7620  
agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 7680  
cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 7740  
gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 7800  
gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 7860  
ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 7920  
cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 7980  
cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 8040  
cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 8100  
gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 8160  
taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg aattaaccct 8220  
cactaaaggg aacaaaagct gctgcagggt ccctaactgc caagccccac agtgtgccct 8280  
gaggctgccc cttccttcta gcggctgccc ccactcggct ttgctttccc tagtttcagt 8340  
tacttgcgtt cagccaaggt ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga 8400  
aagagaccag ctttacaggg ggtttatcac agtgcaccct gacagtcgtc agcctcacag 8460  
ggggtttatc acattgcacc ctgacagtcg tcagcctcac agggggttta tcacagtgca 8520  
cccttacaat cattccattt gattcacaat ttttttagtc tctactgtgc ctaacttgta 8580  
agttaaattt gatcagaggt gtgttcccag aggggaaaac agtatataca gggttcagta 8640  
ctatcgcatt tcaggcctcc acctgggtct tggaatgtgt cccccgaggg gtgatgacta 8700  
cctcagttgg atctccacag gtcacagtga cacaagataa ccaagacacc tcccaaggct 8760  
accacaatgg gccgccctcc acgtgcacat ggccggagga actgccatgt cggaggtgca 8820  
agcacacctg cgcatcagag tccttggtgt ggagggaggg accagcgcag cttccagcca 8880  
tccacctgat gaacagaacc tagggaaagc cccagttcta cttacaccag gaaaggc    8937  
<210> 10
<211> 122
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:region BssHII
(711) to ClaI (830) in transfer construct 1 and 2
(pmBCwCNluci and pmBCmCNluci), sequence mBCwCN
<400> 10
cgcgcacggc aagaggcgag gggcggcgcc tgacgaggac gccaaaaatt ttgactagcg 60    
gaggctagaa ggagagagct cggtgcgaga gcgtcagtat taagcggggg agaattagat 120   
cg                                                                122   
<210> 11
<211> 122
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:region BssHII
(711) to ClaI (830) in transfer construct 3,
sequence m2BCwCN
<400> 11
cgcgcacggc aagaggcgag gggcggcgcc tggggaggac gccaaaaatt ttgactagcg 60    
gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 120   
cg                                                                122   
<210> 12
<211> 122
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:region BssHII
(711) to ClaI (830) in wild-type HIV-1 molecular
clone HXB2
<400> 12
cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactatcg 60    
gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 120   
cg                                                                122   
<210> 13
<211> 122
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:region BssHII
(711) to ClaI (830) in wild-type HIV-1 molecular
clone NL4-3
<400> 13
cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg 60    
gaggctagaa ggagagagat gggtgcgaga gcgtcggtat taagcggggg agaattagat 120   
aa                                                                122   
<210> 14
<211> 122
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:consensus
sequence for region BssHII (711) to ClaI (830) in
transfer constructs 1-3 (sequences mBCwCN and
m2BCwCN) and wild-type HIV-1 molecular clones HXB2
and NL4-3
<400> 14
cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg 60    
gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 120   
cg                                                                122   
<210> 15
<211> 6978
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:SIV envelope
encoding vector CMVkan/R-R-SIVenvCTE containing
mutated SIV env gene
<220> 
<221> CDS
<222> (764)..(3400)
<223> mutated lentiviral env from "env-coding" vector
CMVkan/R-R-SIVenvCTE, mutated SIV env
<400> 15
cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60    
caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120   
ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180   
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240   
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300   
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360   
acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420   
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480   
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540   
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600   
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660   
ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720   
gaagacaccg ggaccgatcc agcctccgcg ggccgcgcta agtatgggat gtcttgggaa 780   
tcagctgctt atcgccatct tgcttttaag tgtctatggg atctattgta ctctatatgt 840   
cacagtcttt tatggtgtac cagcttggag gaatgcgaca attcccctct tttgtgcaac 900   
caagaatagg gatacttggg gaacaactca gtgcctacca gataatggtg attattcaga 960   
agtggccctt aatgttacag aaagctttga tgcctggaat aatacagtca cagaacaggc 1020  
aatagaggat gtatggcaac tctttgagac ctcaataaag ccttgtgtaa aattatcccc 1080  
attatgcatt actatgagat gcaataaaag tgagacagat agatggggat tgacaaaatc 1140  
aataacaaca acagcatcaa caacatcaac gacagcatca gcaaaagtag acatggtcaa 1200  
tgagactagt tcttgtatag cccaggataa ttgcacaggc ttggaacaag agcaaatgat 1260  
aagctgtaaa ttcaacatga cagggttaaa aagagacaag aaaaaagagt acaatgaaac 1320  
ttggtactct gcagatttgg tatgtgaaca agggaataac actggtaatg aaagtagatg 1380  
ttacatgaac cactgtaaca cttctgttat ccaagagtct tgtgacaaac attattggga 1440  
tgctattaga tttaggtatt gtgcacctcc aggttatgct ttgcttagat gtaatgacac 1500  
aaattattca ggctttatgc ctaaatgttc taaggtggtg gtctcttcat gcacaaggat 1560  
gatggagaca cagacttcta cttggtttgg ctttaatgga actagagcag aaaatagaac 1620  
ttatatttac tggcatggta gggataatag gactataatt agtttaaata agtattataa 1680  
tctaacaatg aaatgtagaa gaccaggaaa taagacagtt ttaccagtca ccattatgtc 1740  
tggattggtt ttccactcac aaccaatcaa tgataggcca aagcaggcat ggtgttggtt 1800  
tggaggaaaa tggaaggatg caataaaaga ggtgaagcag accattgtca aacatcccag 1860  
gtatactgga actaacaata ctgataaaat caatttgacg gctcctggag gaggagatcc 1920  
ggaagttacc ttcatgtgga caaattgcag aggagagttc ctctactgta aaatgaattg 1980  
gtttctaaat tgggtagaag ataggaatac agctaaccag aagccaaagg aacagcataa 2040  
aaggaattac gtgccatgtc atattagaca aataatcaac acttggcata aagtaggcaa 2100  
aaatgtttat ttgcctccaa gagagggaga cctcacgtgt aactccacag tgaccagtct 2160  
catagcaaac atagattgga ttgatggaaa ccaaactaat atcaccatga gtgcagaggt 2220  
ggcagaactg tatcgattgg aattgggaga ttataaatta gtagagatca ctccaattgg 2280  
cttggccccc acagatgtga agaggtacac tactggtggc acctcaagaa ataaaagagg 2340  
ggtctttgtg ctagggttct tgggttttct cgcaacggca ggttctgcaa tgggagccgc 2400  
cagcctgacc ctcacggcac agtcccgaac tttattggct gggatagtcc aacagcagca 2460  
acagctgttg gacgtggtca agagacaaca agaattgttg cgactgaccg tctggggaac 2520  
aaagaacctc cagactaggg tcactgccat cgagaagtac ttaaaggacc aggcgcagct 2580  
gaatgcttgg ggatgtgcgt ttagacaagt ctgccacact actgtaccat ggccaaatgc 2640  
aagtctaaca ccaaagtgga acaatgagac ttggcaagag tgggagcgaa aggttgactt 2700  
cttggaagaa aatataacag ccctcctaga ggaggcacaa attcaacaag agaagaacat 2760  
gtatgaatta caaaagttga atagctggga tgtgtttggc aattggtttg accttgcttc 2820  
ttggataaag tatatacaat atggagttta tatagttgta ggagtaatac tgttaagaat 2880  
agtgatctat atagtacaaa tgctagctaa gttaaggcag gggtataggc cagtgttctc 2940  
ttccccaccc tcttatttcc agcagaccca tatccaacag gacccggcac tgccaaccag 3000  
agaaggcaaa gaaagagacg gtggagaagg cggtggcaac agctcctggc cttggcagat 3060  
agaatatatc cactttctta ttcgtcagct tattagactc ttgacttggc tattcagtaa 3120  
ctgtaggact ttgctatcga gagtatacca gatcctccaa ccaatactcc agaggctctc 3180  
tgcgacccta cagaggattc gagaagtcct caggactgaa ctgacctacc tacaatatgg 3240  
gtggagctat ttccatgagg cggtccaggc cgtctggaga tctgcgacag agactcttgc 3300  
gggcgcgtgg ggagacttat gggagactct taggagaggt ggaagatgga tactcgcaat 3360  
ccccaggagg attagacaag ggcttgagct cactctcttg tgagggacag agaattcgga 3420  
tccactagtt ctagactcga gggggggccc ggtacgagcg cttagctagc tagagaccac 3480  
ctcccctgcg agctaagctg gacagccaat gacgggtaag agagtgacat ttttcactaa 3540  
cctaagacag gagggccgtc agagctactg cctaatccaa agacgggtaa aagtgataaa 3600  
aatgtatcac tccaacctaa gacaggcgca gcttccgagg gatttgtcgt ctgttttata 3660  
tatatttaaa agggtgacct gtccggagcc gtgctgcccg gatgatgtct tggtctagac 3720  
tcgagggggg gcccggtacg atccagatct gctgtgcctt ctagttgcca gccatctgtt 3780  
gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 3840  
taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 3900  
ggggtggggc agcacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 3960  
gcggtgggct ctatgggtac ccaggtgctg aagaattgac ccggttcctc ctgggccaga 4020  
aagaagcagg cacatcccct tctctgtgac acaccctgtc cacgcccctg gttcttagtt 4080  
ccagccccac tcataggaca ctcatagctc aggagggctc cgccttcaat cccacccgct 4140  
aaagtacttg gagcggtctc tccctccctc atcagcccac caaaccaaac ctagcctcca 4200  
agagtgggaa gaaattaaag caagataggc tattaagtgc agagggagag aaaatgcctc 4260  
caacatgtga ggaagtaatg agagaaatca tagaatttct tccgcttcct cgctcactga 4320  
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 4380  
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 4440  
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 4500  
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 4560  
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 4620  
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 4680  
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4740  
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4800  
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4860  
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4920  
gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4980  
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 5040  
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 5100  
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 5160  
cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 5220  
gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 5280  
tctatttcgt tcatccatag ttgcctgact ccgggggggg ggggcgctga ggtctgcctc 5340  
gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 5400  
tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 5460  
tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 5520  
cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg 5580  
ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa 5640  
ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa 5700  
tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc 5760  
gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt 5820  
atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg 5880  
catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc 5940  
atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct 6000  
gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc 6060  
atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc 6120  
ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt 6180  
cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt 6240  
ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa 6300  
tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa 6360  
atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg 6420  
gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga 6480  
tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttcccc 6540  
ccccccccat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 6600  
atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 6660  
tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 6720  
gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 6780  
ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 6840  
gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 6900  
actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 6960  
catcagattg gctattgg                                               6978  
<210> 16
<211> 879        
<212> PRT
<213> Artificial Sequence
<220> 
<223> mutated lentiviral env from "env-coding" vector
CMVkan/R-R-SIVenvCTE, mutated SIV env
<400> 16
Met Gly Cys Leu Gly Asn Gln Leu Leu Ile Ala Ile Leu Leu Leu Ser  
1               5                  10                  15      
Val Tyr Gly Ile Tyr Cys Thr Leu Tyr Val Thr Val Phe Tyr Gly Val  
20                  25                  30          
Pro Ala Trp Arg Asn Ala Thr Ile Pro Leu Phe Cys Ala Thr Lys Asn  
35                  40                  45              
Arg Asp Thr Trp Gly Thr Thr Gln Cys Leu Pro Asp Asn Gly Asp Tyr  
50                  55                  60                  
Ser Glu Val Ala Leu Asn Val Thr Glu Ser Phe Asp Ala Trp Asn Asn  
65                  70                  75                  80  
Thr Val Thr Glu Gln Ala Ile Glu Asp Val Trp Gln Leu Phe Glu Thr  
85                  90                  95      
Ser Ile Lys Pro Cys Val Lys Leu Ser Pro Leu Cys Ile Thr Met Arg  
100                 105                 110          
Cys Asn Lys Ser Glu Thr Asp Arg Trp Gly Leu Thr Lys Ser Ile Thr  
115                 120                 125              
Thr Thr Ala Ser Thr Thr Ser Thr Thr Ala Ser Ala Lys Val Asp Met  
130                 135                 140                  
Val Asn Glu Thr Ser Ser Cys Ile Ala Gln Asp Asn Cys Thr Gly Leu  
145                 150                 155                 160  
Glu Gln Glu Gln Met Ile Ser Cys Lys Phe Asn Met Thr Gly Leu Lys  
165                 170                 175      
Arg Asp Lys Lys Lys Glu Tyr Asn Glu Thr Trp Tyr Ser Ala Asp Leu  
180                 185                 190          
Val Cys Glu Gln Gly Asn Asn Thr Gly Asn Glu Ser Arg Cys Tyr Met  
195                 200                 205              
Asn His Cys Asn Thr Ser Val Ile Gln Glu Ser Cys Asp Lys His Tyr  
210                 215                 220                  
Trp Asp Ala Ile Arg Phe Arg Tyr Cys Ala Pro Pro Gly Tyr Ala Leu  
225                 230                 235                 240  
Leu Arg Cys Asn Asp Thr Asn Tyr Ser Gly Phe Met Pro Lys Cys Ser  
245                 250                 255      
Lys Val Val Val Ser Ser Cys Thr Arg Met Met Glu Thr Gln Thr Ser  
260                 265                 270          
Thr Trp Phe Gly Phe Asn Gly Thr Arg Ala Glu Asn Arg Thr Tyr Ile  
275                 280                 285              
Tyr Trp His Gly Arg Asp Asn Arg Thr Ile Ile Ser Leu Asn Lys Tyr  
290                 295                 300                  
Tyr Asn Leu Thr Met Lys Cys Arg Arg Pro Gly Asn Lys Thr Val Leu  
305                 310                 315                 320  
Pro Val Thr Ile Met Ser Gly Leu Val Phe His Ser Gln Pro Ile Asn  
325                 330                 335      
Asp Arg Pro Lys Gln Ala Trp Cys Trp Phe Gly Gly Lys Trp Lys Asp  
340                 345                 350          
Ala Ile Lys Glu Val Lys Gln Thr Ile Val Lys His Pro Arg Tyr Thr  
355                 360                 365              
Gly Thr Asn Asn Thr Asp Lys Ile Asn Leu Thr Ala Pro Gly Gly Gly  
370                 375                 380                  
Asp Pro Glu Val Thr Phe Met Trp Thr Asn Cys Arg Gly Glu Phe Leu  
385                 390                 395                 400  
Tyr Cys Lys Met Asn Trp Phe Leu Asn Trp Val Glu Asp Arg Asn Thr  
405                 410                 415      
Ala Asn Gln Lys Pro Lys Glu Gln His Lys Arg Asn Tyr Val Pro Cys  
420                 425                 430          
His Ile Arg Gln Ile Ile Asn Thr Trp His Lys Val Gly Lys Asn Val  
435                 440                 445              
Tyr Leu Pro Pro Arg Glu Gly Asp Leu Thr Cys Asn Ser Thr Val Thr  
450                 455                 460                  
Ser Leu Ile Ala Asn Ile Asp Trp Ile Asp Gly Asn Gln Thr Asn Ile  
465                 470                 475                 480  
Thr Met Ser Ala Glu Val Ala Glu Leu Tyr Arg Leu Glu Leu Gly Asp  
485                 490                 495      
Tyr Lys Leu Val Glu Ile Thr Pro Ile Gly Leu Ala Pro Thr Asp Val  
500                 505                 510          
Lys Arg Tyr Thr Thr Gly Gly Thr Ser Arg Asn Lys Arg Gly Val Phe  
515                 520                 525              
Val Leu Gly Phe Leu Gly Phe Leu Ala Thr Ala Gly Ser Ala Met Gly  
530                 535                 540                  
Ala Ala Ser Leu Thr Leu Thr Ala Gln Ser Arg Thr Leu Leu Ala Gly  
545                 550                 555                 560  
Ile Val Gln Gln Gln Gln Gln Leu Leu Asp Val Val Lys Arg Gln Gln  
565                 570                 575      
Glu Leu Leu Arg Leu Thr Val Trp Gly Thr Lys Asn Leu Gln Thr Arg  
580                 585                 590          
Val Thr Ala Ile Glu Lys Tyr Leu Lys Asp Gln Ala Gln Leu Asn Ala  
595                 600                 605              
Trp Gly Cys Ala Phe Arg Gln Val Cys His Thr Thr Val Pro Trp Pro  
610                 615                 620                  
Asn Ala Ser Leu Thr Pro Lys Trp Asn Asn Glu Thr Trp Gln Glu Trp  
625                 630                 635                 640  
Glu Arg Lys Val Asp Phe Leu Glu Glu Asn Ile Thr Ala Leu Leu Glu  
645                 650                 655      
Glu Ala Gln Ile Gln Gln Glu Lys Asn Met Tyr Glu Leu Gln Lys Leu  
660                 665                 670          
Asn Ser Trp Asp Val Phe Gly Asn Trp Phe Asp Leu Ala Ser Trp Ile  
675                 680                 685              
Lys Tyr Ile Gln Tyr Gly Val Tyr Ile Val Val Gly Val Ile Leu Leu  
690                 695                 700                  
Arg Ile Val Ile Tyr Ile Val Gln Met Leu Ala Lys Leu Arg Gln Gly  
705                 710                 715                 720  
Tyr Arg Pro Val Phe Ser Ser Pro Pro Ser Tyr Phe Gln Gln Thr His  
725                 730                 735      
Ile Gln Gln Asp Pro Ala Leu Pro Thr Arg Glu Gly Lys Glu Arg Asp  
740                 745                 750          
Gly Gly Glu Gly Gly Gly Asn Ser Ser Trp Pro Trp Gln Ile Glu Tyr  
755                 760                 765              
Ile His Phe Leu Ile Arg Gln Leu Ile Arg Leu Leu Thr Trp Leu Phe  
770                 775                 780                  
Ser Asn Cys Arg Thr Leu Leu Ser Arg Val Tyr Gln Ile Leu Gln Pro  
785                 790                 795                 800  
Ile Leu Gln Arg Leu Ser Ala Thr Leu Gln Arg Ile Arg Glu Val Leu  
805                 810                 815      
Arg Thr Glu Leu Thr Tyr Leu Gln Tyr Gly Trp Ser Tyr Phe His Glu  
820                 825                 830          
Ala Val Gln Ala Val Trp Arg Ser Ala Thr Glu Thr Leu Ala Gly Ala  
835                 840                 845              
Trp Gly Asp Leu Trp Glu Thr Leu Arg Arg Gly Gly Arg Trp Ile Leu  
850                 855                 860                  
Ala Ile Pro Arg Arg Ile Arg Gln Gly Leu Glu Leu Thr Leu Leu      
865                 870                 875                      
<210> 17
<211> 271
<212> PRT
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:translation of
complementary strand positions 6426-5611 of
vector CMVkan/R-R-SIVenvCTE (SEQ ID NO:15)
<400> 17
Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn  
1               5                  10                  15      
Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn  
20                  25                  30          
Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp  
35                  40                  45              
Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp  
50                  55                  60                  
Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro  
65                  70                  75                  80  
Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu  
85                  90                  95      
Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu  
100                 105                 110          
Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu  
115                 120                 125              
Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp  
130                 135                 140                  
Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu  
145                 150                 155                 160  
Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu  
165                 170                 175      
Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser  
180                 185                 190          
Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu  
195                 200                 205              
Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp  
210                 215                 220                  
Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser  
225                 230                 235                 240  
Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro  
245                 250                 255      
Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe      
260                 265                 270          
<210> 18
<211> 2637
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:coding sequence of
mutated lentiviral env from "env-coding" vector
CMVkan/R-R-SIVenvCTE, coding sequence of
mutated SIV env in vector CMVkan/R-R-SIVgp160 CTE
<400> 18
atgggatgtc ttgggaatca gctgcttatc gccatcttgc ttttaagtgt ctatgggatc 60    
tattgtactc tatatgtcac agtcttttat ggtgtaccag cttggaggaa tgcgacaatt 120   
cccctctttt gtgcaaccaa gaatagggat acttggggaa caactcagtg cctaccagat 180   
aatggtgatt attcagaagt ggcccttaat gttacagaaa gctttgatgc ctggaataat 240   
acagtcacag aacaggcaat agaggatgta tggcaactct ttgagacctc aataaagcct 300   
tgtgtaaaat tatccccatt atgcattact atgagatgca ataaaagtga gacagataga 360   
tggggattga caaaatcaat aacaacaaca gcatcaacaa catcaacgac agcatcagca 420   
aaagtagaca tggtcaatga gactagttct tgtatagccc aggataattg cacaggcttg 480   
gaacaagagc aaatgataag ctgtaaattc aacatgacag ggttaaaaag agacaagaaa 540   
aaagagtaca atgaaacttg gtactctgca gatttggtat gtgaacaagg gaataacact 600   
ggtaatgaaa gtagatgtta catgaaccac tgtaacactt ctgttatcca agagtcttgt 660   
gacaaacatt attgggatgc tattagattt aggtattgtg cacctccagg ttatgctttg 720   
cttagatgta atgacacaaa ttattcaggc tttatgccta aatgttctaa ggtggtggtc 780   
tcttcatgca caaggatgat ggagacacag acttctactt ggtttggctt taatggaact 840   
agagcagaaa atagaactta tatttactgg catggtaggg ataataggac tataattagt 900   
ttaaataagt attataatct aacaatgaaa tgtagaagac caggaaataa gacagtttta 960   
ccagtcacca ttatgtctgg attggttttc cactcacaac caatcaatga taggccaaag 1020  
caggcatggt gttggtttgg aggaaaatgg aaggatgcaa taaaagaggt gaagcagacc 1080  
attgtcaaac atcccaggta tactggaact aacaatactg ataaaatcaa tttgacggct 1140  
cctggaggag gagatccgga agttaccttc atgtggacaa attgcagagg agagttcctc 1200  
tactgtaaaa tgaattggtt tctaaattgg gtagaagata ggaatacagc taaccagaag 1260  
ccaaaggaac agcataaaag gaattacgtg ccatgtcata ttagacaaat aatcaacact 1320  
tggcataaag taggcaaaaa tgtttatttg cctccaagag agggagacct cacgtgtaac 1380  
tccacagtga ccagtctcat agcaaacata gattggattg atggaaacca aactaatatc 1440  
accatgagtg cagaggtggc agaactgtat cgattggaat tgggagatta taaattagta 1500  
gagatcactc caattggctt ggcccccaca gatgtgaaga ggtacactac tggtggcacc 1560  
tcaagaaata aaagaggggt ctttgtgcta gggttcttgg gttttctcgc aacggcaggt 1620  
tctgcaatgg gagccgccag cctgaccctc acggcacagt cccgaacttt attggctggg 1680  
atagtccaac agcagcaaca gctgttggac gtggtcaaga gacaacaaga attgttgcga 1740  
ctgaccgtct ggggaacaaa gaacctccag actagggtca ctgccatcga gaagtactta 1800  
aaggaccagg cgcagctgaa tgcttgggga tgtgcgttta gacaagtctg ccacactact 1860  
gtaccatggc caaatgcaag tctaacacca aagtggaaca atgagacttg gcaagagtgg 1920  
gagcgaaagg ttgacttctt ggaagaaaat ataacagccc tcctagagga ggcacaaatt 1980  
caacaagaga agaacatgta tgaattacaa aagttgaata gctgggatgt gtttggcaat 2040  
tggtttgacc ttgcttcttg gataaagtat atacaatatg gagtttatat agttgtagga 2100  
gtaatactgt taagaatagt gatctatata gtacaaatgc tagctaagtt aaggcagggg 2160  
tataggccag tgttctcttc cccaccctct tatttccagc agacccatat ccaacaggac 2220  
ccggcactgc caaccagaga aggcaaagaa agagacggtg gagaaggcgg tggcaacagc 2280  
tcctggcctt ggcagataga atatatccac tttcttattc gtcagcttat tagactcttg 2340  
acttggctat tcagtaactg taggactttg ctatcgagag tataccagat cctccaacca 2400  
atactccaga ggctctctgc gaccctacag aggattcgag aagtcctcag gactgaactg 2460  
acctacctac aatatgggtg gagctatttc catgaggcgg tccaggccgt ctggagatct 2520  
gcgacagaga ctcttgcggg cgcgtgggga gacttatggg agactcttag gagaggtgga 2580  
agatggatac tcgcaatccc caggaggatt agacaagggc ttgagctcac tctcttg  2637 
<210> 19
<211> 813
<212> DNA
<213> Artificial Sequence
<220> 
<223> Description of Artificial Sequence:coding sequence of
complementary strand positions 6426-5611 of vector
CMVkan/R-R-SIVenvCTE (SEQ ID NO:15)
<400> 19
atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat 60    
gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 120   
tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc 180   
gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 240   
cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 300   
atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 360   
gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 420   
tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 480   
gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 540   
gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 600   
cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 660   
ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 720   
ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 780   
ttgcagtttc atttgatgct cgatgagttt ttc                              813   
【手続補正２】
【補正対象書類名】明細書
【補正対象項目名】０１２２
【補正方法】変更
【補正の内容】
【０１２２】
【図１】変異したHIV-1 gag/pol分子クローンのDNA配列(配列番号：1)。gagpolターミネーターは、配列番号：1の4305位〜4307位に位置している。
【図２】野生型（配列番号：2）およびpCMVgagpolBNkanにおける変異したpol領域（配列番号：3）の配列の比較。図における位置#1は、プラスミドpCMVgagpolBNkanにおける2641位である。比較は、1872位のgagイニシエーターATGから始まっている。
【図３】変異したSIV gag分子クローンのDNA配列(SIVgagDX)（配列番号：4）。
【図４】SIVgagDXにおける変異したSIV gag DNA配列の、サル(マカーク)免疫不全ウイルス単離239、クローンλsiv 239-1(GenBankアクセッション番号M33262)由来の野生型SIV配列との比較。コンセンサス配列を配列番号：5に示す。
【図５】レンチウイルス系の見本版のいくつかの構成要素の模式図。BGHポリ(A)：ウシ成長ホルモンポリ(A)シグナル；MSD：変異したスプライス供与部位；Ψ：キャプシド形成シグナル；SD、スプライス供与部位；SA、スプライス受容部位；「X」はこの遺伝子の翻訳が起こらないように一部分のgag遺伝子配列のATGコドンが変異していることを示している。
【図６】パッケージング構築物pCMVgagpolBNkanの模式図。
【図７】転移構築物1：pmBCwCNluciの模式図。矢印により示されているように、パッケージングシグナル、CMVプロモーターおよびルシフェラーゼ遺伝子についてのコード領域は、プロモーターおよびポリアデニル化シグナルを供給している5' HIV-1 LTRおよび3' HIV-1 LTRに隣接している。3つの連続する矢印は、それぞれ、LTRのU5領域、R領域、およびU3領域を示す。LTRの転写される部分は黒で示されている。いくつかの制限エンドヌクレアーゼ切断部位もまた示されている。
【図８】転移構築物2：pmBCmCNluciの模式図。記号は上の通りである。
【図９】パッケージング構築物pCMVgagpolBNkanのDNA配列（配列番号：6）。相補鎖7814位〜7002位の翻訳を配列番号：7に示す。
【図１０】転移構築物1：pmBCwCNluciのDNA配列（配列番号：8）。
【図１１】転移構築物2：pmBCmCNluciのDNA配列（配列番号：9）。
【図１２】野生型HIV-1分子クローンHXB2（配列番号：12）およびNL4-3（配列番号：13）、ならびに転移構築物における領域BssHII(711)〜ClaI(830)のヌクレオチド配列。Gagタンパク質についての翻訳イニシエーターシグナル(ATG)は下線が引かれている。pmBCwCNluciおよびpmBCmCNluci(転移構築物1および転移構築物2)は、配列mBCwCN（配列番号：10）を含む。転移構築物3は、配列m2BCwCN（配列番号：11）を含む。配列mBCwCNと比較して、m2BCwCNは、5'スプライス部位領域に異なる変異をもち、かつ無傷のGag ATGを有する。コンセンサス配列を配列番号：14に示す。
【図１３】pCMVgagpolBNkan(図ではpCMVBNKanと名付けられている)での一過性トランスフェクションにより細胞から放出される、gagタンパク質のレベルを示す棒グラフ。
【図１４】Rev非依存性gag-pol HIV-1ベクターpCMVgagpolBNkan(図ではpCMVBNKanと名付けられている)由来の逆転写酵素活性を示す棒グラフ。
【図１５】pCMVgagpolBNkan Rev非依存性gag-HIV-1基盤レトロウイルスベクターで形質導入された細胞において検出される、p24 Gagタンパク質のナノグラムあたりのルシフェラーゼ量を示す棒グラフ。結果は、pCMVgagpolBNkan Rev非依存性gag-HIV-1基盤レトロウイルスベクターが、(A)293細胞、(B)ヒトリンパ球様細胞、(C)ヒト骨髄細胞(U937)、ならびに（D）初代ヒトマクロファージのような非分裂細胞において、高形質導入効率を見せることを示している。
【図１６】SIVエンベロープをコードするベクターCMVkan/R-R-SIVgp160CTEの模式図。
【図１７】変異型SIV env遺伝子を含むSIVエンベロープをコードするベクターCMVkan/R-R-SIVgp160CTE（配列番号：15）のDNA配列（翻訳を配列番号：16に示す）。相補鎖6426位〜5611位の翻訳を配列番号：17に示す。コード領域を配列番号：18および19にそれぞれ示す。