【手続補正書】
【提出日】平成16年2月4日(2004.2.4)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０１２７
【補正方法】変更
【補正の内容】
【０１２７】
【配列表】
SEQUENCE LISTING
<110> Bradley, John D.
Thompson, Craig M.
Moore, Jeffrey B.
Wobbe, C. Richard
Bailey, David A.
<120> Regulated Gene Expression in Yeast and
Methods of Use
<130> 0342/1D469-US4
<140> 09/573,322
<141> 2000-05-18
<150> 09/404,066
<151> 1999-09-23
<150> 09/138,024
<151> 1998-08-21
<150> 60/056,719
<151> 1997-08-22
<160> 30
<170> FastSEQ for Windows Version 3.0
<210> 1
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> LexA operator
<400> 1
tactgatgta catacagta                                                 19
<210> 2
<211> 52
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial LexA operator
<400> 2
tcgagtactg tatgtacata cagtaccatg acatacatgt atgtcatgag ct            52
<210> 3
<211> 42
<212> DNA
<213> ACE1 binding siteArtificial Sequence
<220>
<223> ACE1 binding site
<400> 3
taagtctttt ttgctggaac ggttgagcgg aaaagacgca tc                       42
<210> 4
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> ROX-A PCR primer
<400> 4
tcacacaaaa gaacgcag                                                  18
<210> 5
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> ROX-B PCR primer
<400> 5
gatgacagct gtggtagg                                                  18
<210> 6
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> ROX-C PCR primer
<400> 6
tcttgccata tggatctg                                                  18
<210> 7
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> UBR-A PCR primer
<400> 7
atcttcggac aaaggcag                                                  18
<210> 8
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> UBR-B PCR primer
<400> 8
gtgtaatttt cgggatcg                                                  18
<210> 9
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> ROX-C PCR Primer
<400> 9
tcttgccata tggatctg                                                  18
<210> 10
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> SLF-E PCR primer
<400> 10
gcgctgcagg tcgacttagc aggcagtttg aac                                 33
<210> 11
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> SLF-F PCR primer
<400> 11
gcgctgcagg catgcactcc tttccaattg tgc                                 33
<210> 12
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> SLF-G PCR primer
<400> 12
gcgagctcgg taccccatac ccctaactct ag                                  32
<210> 13
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> SLF-H PCR primer
<400> 13
gcggatcccg gggctctctc gtttatttaa cg                                  32
<210> 14
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> HISGCH PCR primer
<400> 14
gatttggtct ctaccggc                                                  18
<210> 15
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> SLF-D PCR primer
<400> 15
gacagtatcg taattacg                                                  18
<210> 16
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> PCR primer 2b
<400> 16
ccagactacg cttcgatatc g                                              21
<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> PCR primer 2a
<400> 17
cacactaaaa catcgatatt                                                20
<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> Universal HIS3-2STEP PCR primer
<400> 18
caggcatgca agcttggcgt                                                20
<210> 19
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Primer C typical sequence
<220>
<221> unsure
<222> (28)...(34)
<223> n is a or g or c or t/u
<400> 19
acaaatacac acactaaatt aataatgnnn nnnn                                34
<210> 20
<211> 7102
<212> DNA
<213> Artificial Sequence
<220>
<223> ZM195 plasmid
<400> 20
gaattaattc gagctcggta ccggtgatct tcgctcggcc acaaatcccc tggatatcat     60
tggcctgtcg aggtatcggc cgcgtggaac taccgggaat tactatgcaa aacaattgga    120
aatctggtag gaaaaccttg ttctagaact tggcgattgc tgacaaagaa gaaaagggcc    180
tattgttgct gcctcttttg ttgttcttcc tcgtattgtc ttgccggtgt tctttgtgtc    240
ttttgtgtgt aggttcttac tattatagtg ctctttgcta ttatattttc ttcgttttca    300
ctttgcgtaa tgtaacggtc ttaaacaaag tttttttttt ttcgctcttg cattttcctt    360
ttctgctcta tcttatttgc taattgtagt ttcagaagtt ttaccttaaa tatagcacta    420
ttttccagtt ttaatgtttc ttctcattgc tttcttttat aattttcgca tataattata    480
catttacggt gtcttaactc tccctcttca cccctcatta ttccagaaaa tactaatact    540
tcttcacaca aaagaacgca gttagacaat caacaatgac tagtagtttt tcttgaacca    600
aagaaaggtc accagaggca atagactctt caatctcatt gattctttgc ttggcttctg    660
cagtggacga gaacttggcc tttttgccta acttctcctc aatttggttg ttttttctct    720
tgatttgagc atccaattgc ttaatagagt cgtgaatgtt gcttctacgg gttttcaagt    780
cagcttggat cttgatgatc tccttgttct tatcctgtaa cttcttacgt tcttgttggg    840
tggtatcgtt gacctggtgt tgatcgattt gctttctaat taaaccgatt tcagtgtcga    900
tttttttcaa ttgaacgtta agagtgtcca atttcttgtc tctaacggag acatctgggc    960
gcttgaactt gtgttgttgg gaggacatgg caatggctgt gttgttagaa aatatgctat   1020
tacgttgata aaaggaggaa aggtgaaatc agttcaaaaa tgtgaatgaa actgaacgaa   1080
gaaatgacca gaatgagtga aaaatggaga tggaggggca aaatgaaaaa aaaaaaaagg   1140
atgaacctaa aatagaaaat agactccgtc gtactttaat gctatgtata acgcaaccaa   1200
gcaattttcg aaactcaatt tggcttataa atgttcgaga taaaatgcga attacgtgtt   1260
caacgtcgtc gagatcagtt attttttttc acgccacagt gcgggtaagc aatttttcgc   1320
gtaccaccac cattacacat gtataatgta tataggctta ttatgtatgt ttgtgctact   1380
ttatatgacg gttatttaca agttagaata ttatctatta acaatgcagt agccacgctt   1440
acgtttagtg agtcaacaat gggttctggg gcccgattgc ctttctcaat gccaccaaag   1500
ggaatttcga cgaagaagtc actcctcatc ttcaaattcg ttcttacgcc ctggctttcg   1560
ttccccacca ctagaacaac aggcagctcg ttacataatc cgttcaaatc gtgcatgcta   1620
atagtttttc caacagtgta tttttctgac gtggcattag ctaagtggct tgtaataaac   1680
gtccagccac ccatttcttg tgatttagta aaaaactcta acggtttatc aacgtaaaat   1740
atgggcagaa gttcgagggc cccactgctt gtcttggaca ccacaggcgt caaaggagag   1800
cagtttcttc tcgacatcac aatgaagtca acccccagga agtaagcgct tctaataatg   1860
gcaccgatat tgtgagggtc agttatttca tccagatata acccgagagg aaacttctta   1920
gcgtctgttt tcgtaccata aggcagttca tgaggtatat tttcgttatt gaagcccagc   1980
tcgtgaatgc ttaatgctgc tgaactggtg tccatgtcgc ctaggtacgc aatctccaca   2040
ggctgcaaag gttttgtctc aagagcaatg ttattgtgca ccccgtaatt ggtcaacaag   2100
tttaatctgt gcttgtccac cagctctgtc gtaaccttca gttcatcgac tatctgaaga   2160
aatttactag gaatagtgcc atggtacagc aaccgagaat ggcaatttct actcgggttc   2220
agcaacgctg cataaacgct gttggtgccg tagacatatt cgaagatagg attatcattc   2280
ataagtttca gagcaatgtc cttattctgg aacttggatt tatggctctt ttggtttaat   2340
ttcgcctgat tcttgatctc ctttagcttc tcgacgtggg cctttttctt gccatatgga   2400
tctgaattct agtctttttt gctggaacgg ttgagcggaa aagacgcatc gaattcgagc   2460
tcgttagcga ttggcattat cacataatga attatacatt atataaagta atgtgatttc   2520
ttcgaagaat atactaaaaa atgagcaggc aagataaacg aaggcaaagg acggtatcga   2580
tatcaatgaa tcctaaatcc tctacaccta agattccaag acccaagaac gcatttattc   2640
tgttcagaca gcactaccac aggatcttaa tagacgaatg gaccgctcaa ggtgtggaaa   2700
taccccataa ttcaaacatt tctaaaatta ttggtacgaa gtggaagggc ttacaaccgg   2760
aagataaggc acactgggaa aatctagcgg agaaggagaa actagaacat gaaaggaagt   2820
atcctgaata caaatacaag ccggtaagaa agtctaagaa gaagcaacta cttttgaagg   2880
aaatcgagca acagcagcag caacaacaga aagaacagca gcagcagaaa cagtcacaac   2940
cgcaattaca acagcccttt aacaacaata tagttcttat gaaaagagca cattctcttt   3000
caccatcttc ctcggtgtca agctcgaaca gctatcagtt ccaattgaac aatgatctta   3060
agaggttgcc tattccttct gttaatactt ctaactatat ggtctccaga tcctctagag   3120
tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat   3180
tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg   3240
ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag   3300
tcgggaaacc tgtcgtgcca ggggggatcc actagttcta gagtcgaccg gcatgcaagc   3360
ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca   3420
cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa   3480
ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag   3540
ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc   3600
gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct   3660
cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg   3720
tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc   3780
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga   3840
aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct   3900
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg   3960
gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag   4020
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat   4080
cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac   4140
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac   4200
tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc   4260
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt   4320
tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc   4380
ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg   4440
agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca   4500
atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca   4560
cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag   4620
ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac   4680
ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc   4740
agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct   4800
agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc   4860
gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg   4920
cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc   4980
gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat   5040
tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag   5100
tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat   5160
aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg   5220
cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca   5280
cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga   5340
aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc   5400
ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata   5460
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg   5520
ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc   5580
acgaggccag cttttcaatt caattcatca tttttttttt attctttttt ttgatttcgg   5640
tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag   5700
cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca   5760
gtattcttaa cccaactgca cagaacaaaa acatgcagga aacgaagata aatcatgtcg   5820
aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt   5880
aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag   5940
gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg   6000
gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc   6060
aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa   6120
ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac   6180
ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag   6240
gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga   6300
gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt   6360
attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc   6420
ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat   6480
gtggtctcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg   6540
gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga   6600
tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa   6660
attagagctt caatttaatt atatcagtta ttacccgccc tttcgtctcg cgcgtttcgg   6720
tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta   6780
agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg   6840
gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg   6900
tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag   6960
gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc   7020
gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg   7080
acgttgtaaa acgacggcca gt                                            7102
<210> 21
<211> 7333
<212> DNA
<213> Artificial Sequence
<220>
<223> ZM197 plasmid
<400> 21
gaattaattc gagctcggta ccagttgcca caccacaaaa gtcgaaaaag gctaagaaac     60
caaagaataa ggtactaagt acccaggcgc tactaagacc aacgagattg ccacgaaact    120
agaggaaacc aaattgtaag catagcttaa tccgttttca cgattcataa tataataaat    180
aagaaaagat atatcatata aacgttataa aattaataac cgggtaagtg tagaaaagtg    240
atgcgacggt ttattttctc ttcctcttgc gattgaattt aacttgcaga tagtgaccat    300
aaggcaacta cccagtggca aacagttttg ataacgccca gtacatcaac gagcgagtat    360
aaagactttg gtacatttta aaaaggaaac atatattgtt ttcattgcta gaccctttta    420
gtctcacctc aataaaactg ctttattcct cattgggctt tttattcttt aattttgcat    480
acttatagcg tgaaactggg catttaacaa aagcaaacta ttttaatagt agcatcctgc    540
tttctttgcc cctccttctt attgcgatac attattaagt ttttttacca cctttcttcc    600
tttttcttcg catcttcgga caaaggcagt tgaagtttac tgtatcctat tagttgacta    660
ttttctctca ctgaagtccc taatctttac aggtcacaca aattacatag aacattccaa    720
ctagtagttt ttcttgaacc aaagaaaggt caccagaggc aatagactct tcaatctcat    780
tgattctttg cttggcttct gcagtggacg agaacttggc ctttttgcct aacttctcct    840
caatttggtt gttttttctc ttgatttgag catccaattg cttaatagag tcgtgaatgt    900
tgcttctacg ggttttcaag tcagcttgga tcttgatgat ctccttgttc ttatcctgta    960
acttcttacg ttcttgttgg gtggtatcgt tgacctggtg ttgatcgatt tgctttctaa   1020
ttaaaccgat ttcagtgtcg atttttttca attgaacgtt aagagtgtcc aatttcttgt   1080
ctctaacgga gacatctggg cgcttgaact tgtgttgttg ggaggacatg gcaatggctg   1140
tgttgttaga aaatatgcta ttacgttgat aaaaggagga aaggtgaaat cagttcaaaa   1200
atgtgaatga aactgaacga agaaatgacc agaatgagtg aaaaatggag atggaggggc   1260
aaaatgaaaa aaaaaaaaag gatgaaccta aaatagaaaa tagactccgt cgtactttaa   1320
tgctatgtat aacgcaacca agcaattttc gaaactcaat ttggcttata aatgttcgag   1380
ataaaatgcg aattacgtgt tcaacgtcgt cgagatcagt tatttttttt cacgccacag   1440
tgcgggtaag caatttttcg cgtaccacca ccattacaca tgtataatgt atataggctt   1500
attatgtatg tttgtgctac tttatatgac ggttatttac aagttagaat attatctatt   1560
aacaatgcag tagccacgct tacgtttagt gagtcaacaa tgggttctgg ggcccgattg   1620
cctttctcaa tgccaccaaa gggaatttcg acgaagaagt cactcctcat cttcaaattc   1680
gttcttacgc cctggctttc gttccccacc actagaacaa caggcagctc gttacataat   1740
ccgttcaaat cgtgcatgct aatagttttt ccaacagtgt atttttctga cgtggcatta   1800
gctaagtggc ttgtaataaa cgtccagcca cccatttctt gtgatttagt aaaaaactct   1860
aacggtttat caacgtaaaa tatgggcaga agttcgaggg ccccactgct tgtcttggac   1920
accacaggcg tcaaaggaga gcagtttctt ctcgacatca caatgaagtc aacccccagg   1980
aagtaagcgc ttctaataat ggcaccgata ttgtgagggt cagttatttc atccagatat   2040
aacccgagag gaaacttctt agcgtctgtt ttcgtaccat aaggcagttc atgaggtata   2100
ttttcgttat tgaagcccag ctcgtgaatg cttaatgctg ctgaactggt gtccatgtcg   2160
cctaggtacg caatctccac aggctgcaaa ggttttgtct caagagcaat gttattgtgc   2220
accccgtaat tggtcaacaa gtttaatctg tgcttgtcca ccagctctgt cgtaaccttc   2280
agttcatcga ctatctgaag aaatttacta ggaatagtgc catggtacag caaccgagaa   2340
tggcaatttc tactcgggtt cagcaacgct gcataaacgc tgttggtgcc gtagacatat   2400
tcgaagatag gattatcatt cataagtttc agagcaatgt ccttattctg gaacttggat   2460
ttatggctct tttggtttaa tttcgcctga ttcttgatct cctttagctt ctcgacgtgg   2520
gcctttttct tgccatatgg atctgaattc tagtcttttt tgctggaacg gttgagcgga   2580
aaagacgcat cgaattcgag ctcgttagcg attggcatta tcacataatg aattatacat   2640
tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg caagataaac   2700
gaaggcaaag gacggtatcg ataagcttgg gaattcaaaa tgcccaagaa gaagcggaag   2760
gtccatatgt acccatacga cgttccagac tacgcttctt tgggtggttc tagcccaagc   2820
ttgatatcga attcctgcag cccgggggat cctaacatgt ccgttgctga tgatgattta   2880
ggatctttac aaggtcacat taggagaaca ctgaggtcta ttcataacct cccctatttt   2940
aggtatacga gaggtcctac tgaaagggct gacatgagca gagcccttaa agagttcatt   3000
tacagatatc tatactttgt catttctaac agcggagaga acttacctac tttattcaat   3060
gctcatccaa aacaaaaatt atctaaccca gagcttactg tttttcctga cagtttagaa   3120
gatgctgtgg atattgataa gataacatct caacaaacta ttccgtttta taagatagat   3180
gaatccagaa taggagacgt ccataaacat accggaagaa attgtgggag gaaattcaaa   3240
ataggggaac ccttgtatag gtgtcatgag tgtggttgcg atgatacttg tgtgctttgt   3300
attcattgtt ttaatccaaa agatcatgtg aatcatcatg tttgtaccga tatatgtact   3360
gaattcgata tcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta   3420
tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc   3480
ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg   3540
aaacctgtcg tgccaggggg gatccactag ttctagagtc gaccggcatg caagcttggc   3600
gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa   3660
catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac   3720
attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca   3780
ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc   3840
ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc   3900
aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc   3960
aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag   4020
gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc   4080
gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt   4140
tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct   4200
ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg   4260
ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct   4320
tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat   4380
tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg   4440
ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa   4500
aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt   4560
ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc   4620
tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt   4680
atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta   4740
aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat   4800
ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac   4860
tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg   4920
ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag   4980
tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt   5040
aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt   5100
gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt   5160
tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt   5220
cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct   5280
tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt   5340
ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac   5400
cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa   5460
actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa   5520
ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca   5580
aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct   5640
ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga   5700
atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc   5760
tgctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcca   5820
gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga   5880
aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt   5940
agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta   6000
acccaactgc acagaacaaa aacatgcagg aaacgaagat aaatcatgtc gaaagctaca   6060
tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg   6120
cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg   6180
gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg   6240
actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat   6300
tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac   6360
tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg   6420
ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga   6480
ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact   6540
aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa   6600
agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt   6660
ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct   6720
acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag   6780
gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag   6840
caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct   6900
tcaatttaat tatatcagtt attacccgcc ctttcgtctc gcgcgtttcg gtgatgacgg   6960
tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc   7020
cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct   7080
taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc   7140
gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa   7200
ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg   7260
atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa   7320
aacgacggcc agt                                                      7333
<210> 22
<211> 4949
<212> DNA
<213> Artificial Sequence
<220>
<223> pCU19Srf vector
<400> 22
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca    60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg   120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc   180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc   240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat   300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt   360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgcccgggcg atctagactt   420
aagcgatatc gaagcgtagt ctggaacgtc gtatgggtag gaatcggcca acgcgcgggg   480
agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa   540
cagccaagct ccggatccgt gcctaccacc tcttagcctt agcacaagat gtaaggtgga   600
ctccttctga atgttgtaat cagacagcgt tctaccgtct tctagctgct taccggcaaa   660
gatcaatctt tgttgatctg gagggatacc ttccttgtct tgaattttcg acttaacgtt   720
gtcgatggta tcggaagatt caacttccaa tgttatggtt ttaccggtca aagtcttgac   780
gaaaatctgc ataatatcga tgttttagtg tgtgaatgaa ataggtgtat gttttctttt   840
tgctagacaa taattaggaa caaggtaagg gaactaaagt gtagaataag attaaaaaag   900
aagaacaagt tgaaaaggca agttgaaatt tcaagaaaaa agtcaattga agtacagtaa   960
attgacctga atatatctga gttccgacaa caatgagttt accgaagaga acaatggaat   020
aggaaacttt gaacgaagaa aggaaagcag gaaaggaaaa aatttttagg ctcgagaaca   080
atagggcaaa aaaacaggca acgaacgaac aatggaaaaa cgaaaaaaaa aaaacacaga   140
aaagaatgca gaaagttgta aactgaaaaa aaaaaaaaaa aggtgaacac aggaaaaaaa   200
ataaaaaaaa aaaaaaagga ggacgaaaca aaaaagtgaa aaaaaatgaa aatttttttg   260
gaaaaccaag aaatgaatta tatttccgtg tgagacgaca tcgtcgaata tgattcaggt   320
acccgggctg ttccctagca tgtacgtgag cgtatttcct tttaaaccac gacgctttgt   380
cttcattcaa cgtttcccat tgtttttttc tactattgct ttgctgtggg aaaaacttat   440
cgaaagatga cgactttttc ttaattctcg ttttaagagc ttggtgagcg ctaggagtca   500
ctgccaggta tcgtttgaac acggcattag tcagggaagt cataacacag tcctttcccg   560
caattttctt tttctattac tcttggcctc ctctagtaca ctctatattt ttttatgcct   620
cggtaatgat tttcattttt ttttttccac ctagcggatg actctttttt tttcttagcg   680
attggcatta tcacataatg aattatacat tatataaagt aatgtgattt cttcgaagaa   740
tatactaaaa aatgagcagg caagataaac gaaggcaaag atgacagagc agaaagccct   800
agtaaagcgt attacaaatg aaaccaagat tcagattgcg atctctttaa agggtggtcc   860
cctagcgata gagcactcga tcttcccaga aaaagaggca gaagcagtag cagaacaggc   920
cacacaatcg caagtgatta acgtccacac aggtataggg tttctggacc atatgataca   980
tgctctggcc aagcattccg gctggtcgct aatcgttgag tgcattggtg acttacacat   040
agacgaccat cacaccactg aagactgcgg gattgctctc ggtcaagctt ttaaagaggc   100
cctactggcg cgtggagtaa aaaggtttgg atcaggattt gcgcctttgg atgaggcact   160
ttccagagcg gtggtagatc tttcgaacag gccgtacgca gttgtcgaac ttggtttgca   220
aagggagaaa gtaggagatc tctcttgcga gatgatcccg cattttcttg aaagctttgc   280
agaggctagc agaattaccc tccacgttga ttgtctgcga ggcaagaatg atcatcaccg   340
tagtgagagt gcgttcaagg ctcttgcggt tgccataaga gaagccacct cgcccaatgg   400
taccaacgat gttccctcca ccaaaggtgt tcttatgtag tgacaccgat tatttaaagc   460
tgcagcatac gatatatata catgtgtata tatgtatacc tatgaatgtc agtaagtatg   520
tatacgaaca gtatgatact gaagatgaca aggtaatgca tcattctata cgtgtcattc   580
tgaacgaggc gcgctttcct tttttctttt tgctttttct ttttttttct cttgaactcg   640
agaaaaaaaa tataaaagag atggaggaac gggaaaaagt tagttgtggt gataggtggc   700
aaggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc   760
gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta   820
atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa   880
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat   940
tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg   000
agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc   060
aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt   120
gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag   180
tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc   240
cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc   300
ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt   360
cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt   420
atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc   480
agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa   540
gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa   600
gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg   660
tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga   720
agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg   780
gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg   840
aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt   900
aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact   960
ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat   020
gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg   080
aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg   140
ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat   200
tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc   260
ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt   320
cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc   380
agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga   440
gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc   500
gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa   560
acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta   620
acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg   680
agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg   740
aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat   800
gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt   860
tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa   920
aaataggcgt atcacgaggc cctttcgtc                                     949
<210> 23
<211> 18
<212> DNA
<213> PCArtificial Sequence
<220>
<223> PCR primer
<400> 23
accctggcgc ccaatacg                                                  18
<210> 24
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> SLF-A PCR primer
<400> 24
ctaactctag ctgcattg                                                  18
<210> 25
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> ScBos1-1 oligo
<400> 25
gcggctcgag gggttttctc tcaacattg                                      29
<210> 26
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> ScBos1-2 oligo
<400> 26
gatcgcggcc gcgtaaggct tattgctgcg                                     30
<210> 27
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> ScBos1deIr oligo
<400> 27
ccaccaacgt tcctcacagc atggttgtaa agagc                               35
<210> 28
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> ScBosldelf oligo
<400> 28
aggaacgttg gtggtgcg                                                  18
<210> 29
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> oligo tRNAw
<400> 29
ggaatttcca agatttaatt ggagtcgaaa gctcgcctta                          40
<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> Primer D
<220>
<221> modified_base
<222> (1)...(1)
<223> n is 3' end of HIS3-n
<220>
<221> unsure
<222> (1)...(20)
<223> n is a or g or c or t/u
<400> 30
nnnnnnnnnn nnnnnnnnnn                                                20