【手続補正書】
【提出日】平成15年12月16日(2003.12.16)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０２５９
【補正方法】変更
【補正の内容】
【０２５９】
本明細書において言及されるすべての刊行物、特許および特許出願は、本発明が属する当該分野の当業者のレベルを示しており、これらは、それぞれの個々の刊行物、特許、または特許出願が参照として組み入れられるように特定的および個別に示されている場合と同程度に、参照として本明細書に組み入れられる。
【配列表】
SEQUENCE LISTING
<110>  Invitrogen Corporation
<120>  Compositions and Methods for Recombinational 
Cloning of Nucleic Acid Molecules
<140>  JP   2002-583657
<141>  2002-04-19
<150>  US   60/284,528
<151>  2001-04-19
<160>  21    
<170>  PatentIn version 3.1
<210>  1
<211>  98
<212>  PRT
<213>  Escherichia coli
<400>  1
Met Phe Glu Gln Arg Val Asn Ser Asp Val Leu Thr Val Ser Thr Val 
1               5                   10                  15      
Asn Ser Gln Asp Gln Val Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Gln Ala Leu Lys Asn Tyr Phe Ala Gln Leu Asn Gly Gln Asp Val Asn 
35                  40                  45              
Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Met Val Met Ala Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Leu Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr Gly 
85                  90                  95      
Met Asn 
<210>  2
<211>  93
<212>  PRT
<213>  Klebsiella pneumoniae
<400>  2
Met Phe Glu Gln Arg Val Asn Ser Asp Val Leu Thr Val Ser Thr Val 
1               5                   10                  15      
Asn Ser Gln Asp Gln Val Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Gln Ala Leu Lys Asn Tyr Phe Ala Gln Leu Asn Gly Gln Asp Val Asn 
35                  40                  45              
Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Met Val Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Leu Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys 
85                  90              
<210>  3
<211>  98
<212>  PRT
<213>  Vibrio cholera
<400>  3
Met Phe Glu Gln Asn Leu Thr Ser Glu Ala Leu Thr Val Thr Thr Val 
1               5                   10                  15      
Thr Ser Gln Asp Gln Ile Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Ala Ser Leu Lys Asn Tyr Leu Ala Gln Leu Asn Gly Gln Glu Val Thr 
35                  40                  45              
Glu Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Thr Ile Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Thr Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr Gly 
85                  90                  95      
Met Asn 
<210>  4
<211>  99
<212>  PRT
<213>  Haemophilus influenzae
<400>  4
Met Leu Glu Gln Gln Arg Asn Ser Ala Asp Ala Leu Thr Val Ser Val 
1               5                   10                  15      
Leu Asn Ala Gln Ser Gln Val Thr Ser Lys Pro Leu Arg Asp Ser Val 
20                  25                  30          
Lys Gln Ala Leu Arg Asn Tyr Leu Ala Gln Leu Asp Gly Gln Asp Val 
35                  40                  45              
Asn Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu His Pro Met Leu 
50                  55                  60                  
Asp Met Ile Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Asn 
65                  70                  75                  80  
Met Leu Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr 
85                  90                  95      
Gly Met Gly 
<210>  5
<211>  107
<212>  PRT
<213>  Pseudomonas aeruginosa
<400>  5
Met Thr Thr Met Thr Thr Glu Thr Leu Val Ser Gly Thr Thr Pro Val 
1               5                   10                  15      
Ser Asp Asn Ala Asn Leu Lys Gln His Leu Thr Thr Pro Thr Gln Glu 
20                  25                  30          
Gly Gln Thr Leu Arg Asp Ser Val Glu Lys Ala Leu His Asn Tyr Phe 
35                  40                  45              
Ala His Leu Glu Gly Gln Pro Val Thr Asp Val Tyr Asn Met Val Leu 
50                  55                  60                  
Cys Glu Val Glu Ala Pro Leu Leu Glu Thr Val Met Asn His Val Lys 
65                  70                  75                  80  
Gly Asn Gln Thr Lys Ala Ser Glu Leu Leu Gly Leu Asn Arg Gly Thr 
85                  90                  95      
Leu Arg Lys Lys Leu Lys Gln Tyr Asp Leu Leu 
100                 105         
<210>  6
<211>  98
<212>  PRT
<213>  Salmonella typhimurium
<400>  6
Met Phe Glu Gln Arg Val Asn Ser Asp Val Leu Thr Val Ser Thr Val 
1               5                   10                  15      
Asn Ser Gln Asp Gln Val Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Gln Ala Leu Lys Asn Tyr Phe Ala Gln Leu Asn Gly Gln Asp Val Asn 
35                  40                  45              
Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Met Val Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Leu Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr Gly 
85                  90                  95      
Met Asn 
<210>  7
<211>  15
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Fis binding site
<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  May be any nucleotide
<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  May be any nucleotide
<400>  7
gntyaawwwt tranc                                                      15
<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Fis binding site
<400>  8
agtctgtttt ttatgcaaaa                                                 20
<210>  9
<211>  45
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  9
tattattatc atatgggacg acgtcgaagt catgagcgcc gggat                     45
<210>  10
<211>  58
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucloetide primer
<400>  10
attattaagc ttattaatgg tgatgatggt gatgtttgat ttcaattttg tcccactc       58
<210>  11
<211>  33
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  11
tattattatc atatgtactt gacacttcag gag                                  33
<210>  12
<211>  55
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  oligonucleotide primer
<400>  12
attattaagc ttattaatgg tgatgatggt gatgtgactt cgccttcttc ccatt          55
<210>  13
<211>  36
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  13
tattattatc atatggctaa tatcaaatca gctaag                               36
<210>  14
<211>  33
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  14
attattggat ccattaagcc agtttgttga tct                                  33
<210>  15
<211>  14
<212>  PRT
<213>  Artificial Sequence
<220>
<223>  Amino acids 2 through 15 of E. coli 30S ribosomal protein S20
<400>  15
Ala Asn Ile Lys Ser Ala Lys Lys Arg Ala Ile Gln Ser Glu 
1               5                   10                  
<210>  16
<211>  11
<212>  PRT
<213>  Artificial Sequence
<220>
<223>  Amino acids 2 through 12 of E. coli 50S ribosomal protein L27
<400>  16
Ala His Lys Lys Ala Gly Gly Ser Thr Arg Asn 
1               5                   10      
<210>  17
<211>  37
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  17
gggggctgca ggcaagaaga caaaaatcac cttgcgc                              37
<210>  18
<211>  37
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  18
gggggcccgg gcagaggcag ggagtgggac aaaattg                              37
<210>  19
<211>  51
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  19
ggagggaatt caggaggtat aaattaatgt tcgaacaacg cgtaaattct g              51
<210>  20
<211>  29
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  20
ggaggggatc cttattagtt catgccgta                                       29
<210>  21
<211>  48
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Oligonucleotide primer
<400>  21
ggaaggagat cttgctcaaa atttgagcta cataatactg taaaacac                  48