【手続補正書】
【提出日】平成16年3月29日(2004.3.29)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０５３０
【補正方法】変更
【補正の内容】
【０５３０】
さらに、以下の文書はそれらの全体が参照として本明細書に組み入れられる：1995年6月7日に提出された米国特許出願第08/486,139号（現在は放棄）；1996年6月7日に提出された米国特許出願第08/663,002号（現在は米国特許第5,888,732号）；1998年1月12日に提出された米国特許出願第09/005,476号（現在は米国特許第6,171,861号）；1997年10月24日に提出された米国特許出願第60/065,930号；1998年10月23日に提出された米国特許出願第09/177,387号；1999年4月22日に提出された米国特許出願第09/296,280号（現在は米国特許第6,277,608号）；1999年3月2日に提出された米国特許出願第60/122,389号；1999年3月22日に提出された米国特許出願第60/122,392号；1999年3月23日に提出された米国特許出願第60/126,049号；米国特許出願第09/233,493号（現在は米国特許第6,143,557号）；1999年11月12日に提出された米国特許出願第09/438,358号；2001年4月19日に提出された米国特許出願第60/284,528号；1999年5月28日に提出された米国特許出願第60/136,744号；1999年11月2日に提出された米国特許出願第09/432,085号；2000年2月4日に提出された米国特許出願第09/498,074号；1998年11月13日に提出された米国特許出願第60/108,324号；1999年11月12日に提出された米国特許出願第09/438,358号；2000年3月2日に提出された米国特許出願第09/517,466号；2000年12月11日に提出された米国特許出願第09/732,914号；およびPCT国際公開公報第00/52027号。
【配列表】
SEQUENCE LISTING
<110>  INVITROGEN CORPORATION 
<120>  COMPOSITIONS AND METHODS FOR USE IN ISOLATION OF NUCLEIC ACID MOLECULES
<130>  SK-A0327
<140>  JP   2002-592514
<141>  2002-05-21
<150>  US   60/291,973
<151>  2001-05-21
<160>  63 
<170>  PatentIn version 3.1
<210>  1
<211>  27
<212>  DNA
<213>  attB0
<400>  1
agcctgcttt tttatactaa cttgagc                                         27
<210>  2
<211>  27
<212>  DNA
<213>  attP0
<400>  2
gttcagcttt tttatactaa gttggca                                         27
<210>  3
<211>  27
<212>  DNA
<213>  attL0
<400>  3
agcctgcttt tttatactaa gttggca                                         27
<210>  4
<211>  27
<212>  DNA
<213>  attR0
<400>  4
gttcagcttt tttatactaa cttgagc                                         27
<210>  5
<211>  25
<212>  DNA
<213>  attB1
<400>  5
agcctgcttt tttgtacaaa cttgt                                           25
<210>  6
<211>  27
<212>  DNA
<213>  attP1
<400>  6
gttcagcttt tttgtacaaa gttggca                                         27
<210>  7
<211>  27
<212>  DNA
<213>  attL1
<400>  7
agcctgcttt tttgtacaaa gttggca                                         27
<210>  8
<211>  25
<212>  DNA
<213>  attR1
<400>  8
gttcagcttt tttgtacaaa cttgt                                           25
<210>  9
<211>  25
<212>  DNA
<213>  attB2
<400>  9
acccagcttt cttgtacaaa gtggt                                           25
<210>  10
<211>  27
<212>  DNA
<213>  attP2
<400>  10
gttcagcttt cttgtacaaa gttggca                                         27
<210>  11
<211>  27
<212>  DNA
<213>  attL2
<400>  11
acccagcttt cttgtacaaa gttggca                                         27
<210>  12
<211>  25
<212>  DNA
<213>  attR2
<400>  12
gttcagcttt cttgtacaaa gtggt                                           25
<210>  13
<211>  22
<212>  DNA
<213>  attB5
<400>  13
caactttatt atacaaagtt gt                                              22
<210>  14
<211>  27
<212>  DNA
<213>  attP5
<400>  14
gttcaacttt attatacaaa gttggca                                         27
<210>  15
<211>  24
<212>  DNA
<213>  attL5
<400>  15
caactttatt atacaaagtt ggca                                            24
<210>  16
<211>  25
<212>  DNA
<213>  attR5
<400>  16
gttcaacttt attatacaaa gttgt                                           25
<210>  17
<211>  22
<212>  DNA
<213>  attB11
<400>  17
caacttttct atacaaagtt gt                                              22
<210>  18
<211>  27
<212>  DNA
<213>  attP11
<400>  18
gttcaacttt tctatacaaa gttggca                                         27
<210>  19
<211>  24
<212>  DNA
<213>  attL11
<400>  19
caacttttct atacaaagtt ggca                                            24
<210>  20
<211>  25
<212>  DNA
<213>  attR11
<400>  20
gttcaacttt tctatacaaa gttgt                                           25
<210>  21
<211>  22
<212>  DNA
<213>  attB17
<400>  21
caacttttgt atacaaagtt gt                                              22
<210>  22
<211>  27
<212>  DNA
<213>  attP17
<400>  22
gttcaacttt tgtatacaaa gttggca                                         27
<210>  23
<211>  24
<212>  DNA
<213>  attL17
<400>  23
caacttttgt atacaaagtt ggca                                            24
<210>  24
<211>  25
<212>  DNA
<213>  attR17
<400>  24
gttcaacttt tgtatacaaa gttgt                                           25
<210>  25
<211>  22
<212>  DNA
<213>  attB19
<400>  25
caactttttc gtacaaagtt gt                                              22
<210>  26
<211>  27
<212>  DNA
<213>  attP19
<400>  26
gttcaacttt ttcgtacaaa gttggca                                         27
<210>  27
<211>  24
<212>  DNA
<213>  attL19
<400>  27
caactttttc gtacaaagtt ggca                                            24
<210>  28
<211>  25
<212>  DNA
<213>  attR19
<400>  28
gttcaacttt ttcgtacaaa gttgt                                           25
<210>  29
<211>  22
<212>  DNA
<213>  attB20
<400>  29
caactttttg gtacaaagtt gt                                              22
<210>  30
<211>  27
<212>  DNA
<213>  attP20
<400>  30
gttcaacttt ttggtacaaa gttggca                                         27
<210>  31
<211>  24
<212>  DNA
<213>  attL20
<400>  31
caactttttg gtacaaagtt ggca                                            24
<210>  32
<211>  25
<212>  DNA
<213>  attR20
<400>  32
gttcaacttt ttggtacaaa gttgt                                           25
<210>  33
<211>  22
<212>  DNA
<213>  attB21
<400>  33
caacttttta atacaaagtt gt                                              22
<210>  34
<211>  27
<212>  DNA
<213>  attP21
<400>  34
gttcaacttt ttaatacaaa gttggca                                         27
<210>  35
<211>  24
<212>  DNA
<213>  attL21
<400>  35
caacttttta atacaaagtt ggca                                            24
<210>  36
<211>  25
<212>  DNA
<213>  attR21
<400>  36 
gttcaacttt ttaatacaaa gttgt             25
<210>  37
<211>  2717
<212>  DNA
<213>  Artificial sequence
<220>
<223>  Entry Vector pENTR1A
<220>
<221>  gene
<222>  (67)..(166)
<223>  attL1
<220>
<221>  gene
<222>  (321)..(626)
<223>  ccdB
<220>
<221>  gene
<222>  (655)..(754)
<223>  attL2
<220>
<221>  gene
<222>  (877)..(1686)
<223>  KmR
<220>
<221>  gene
<222>  (1791)..(2364)
<223>  ori
<400>  37
ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc     60
gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat    120
aagcaatgct tttttataat gccaactttg tacaaaaaag caggctttaa aggaaccaat    180
tcagtcgact ggatccggta ccgaattcgc ttactaaaag ccagataaca gtatgcgtat    240
ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca    300
aaaagaggtg tgcttctaga atgcagttta aggtttacac ctataaaaga gagagccgtt    360
atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatagtga    420
tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg    480
tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct    540
ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca    600
ttaacctgat gttctgggga atatagaatt cgcggccgca ctcgagatat ctagacccag    660
ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac    720
aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg    780
tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa    840
ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg    900
tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc    960
gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca   1020
gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc   1080
agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact   1140
cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta   1200
gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtc cctgcgccgg   1260
ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct   1320
caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt   1380
aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg   1440
gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa   1500
ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc   1560
atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa   1620
tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt   1680
ttctaatcag aattggttaa ttggttgtaa cattattcag attgggcccc gttccactga   1740
gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta   1800
atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa   1860
gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact   1920
gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca   1980
tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt   2040
accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg   2100
ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag   2160
cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta   2220
agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat   2280
ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg   2340
tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc   2400
ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac   2460
cgtattaccg ctagcatgga tctcggggac gtctaactac taagcgagag tagggaactg   2520
ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt   2580
gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg   2640
tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa   2700
ctaagcagaa ggccatc                                                  2717
<210>  38
<211>  6464
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pDEST1
<220>
<221>  gene
<222>  (216)..(257)
<223>  Trc promoter
<220>
<221>  gene
<222>  (273)..(393)
<223>  attR1
<220>
<221>  gene
<222>  (647)..(1306)
<223>  CmR
<220>
<221>  gene
<222>  (1426)..(1510)
<223>  inactivated ccdA
<220>
<221>  gene
<222>  (1648)..(1953)
<223>  ccdB
<220>
<221>  gene
<222>  (1994)..(2118)
<223>  attR2
<220>
<221>  gene
<222>  (2598)..(3503)
<223>  ampR
<220>
<221>  gene
<222>  (4104)..(4264)
<223>  ori
<220>
<221>  gene
<222>  (4504)..(4941)
<223>  f1ori (f1 intergenic region)
<220>
<221>  gene
<222>  (5340)..(6420)
<223>  lacIq
<400>  38
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc     60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc    120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc    180
tgaaatgagc tgttgacaat taatcatccg gtccgtataa tctgtggaat tgtgagcggg    240
ataacaattt catcgcgagg taccaagcta tcacaagttt gtacaaaaaa gctgaacgag    300
aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta    360
cataatactg taaaacacaa catatccagt cactatggcg gccgctaagt tggcagcatc    420
acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat    480
aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga    540
cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg    600
cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat    660
cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt    720
tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt    780
aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg    840
cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg    900
ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct    960
ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc   1020
gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt   1080
ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa   1140
cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat   1200
gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct   1260
taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg   1320
cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat   1380
atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt   1440
acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct   1500
ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga   1560
aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt   1620
ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag   1680
agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg   1740
atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac   1800
ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg   1860
ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa   1920
aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag   1980
tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt   2040
tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc   2100
tttcttgtac aaagtggtga tagcttggct gttttggcgg atgagagaag attttcagcc   2160
tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca   2220
gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg   2280
atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga   2340
aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc   2400
ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg   2460
tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg   2520
acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa   2580
atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga   2640
agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc   2700
ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg   2760
gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc   2820
gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat   2880
tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg   2940
acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag   3000
aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa   3060
cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc   3120
gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca   3180
cgatgcctac agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc   3240
tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc   3300
tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg   3360
ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta   3420
tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag   3480
gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga   3540
ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc   3600
tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa   3660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa   3720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc   3780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt   3840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc   3900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac   3960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca   4020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg   4080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag   4140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt   4200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat   4260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc   4320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt   4380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag   4440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca   4500
taattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg   4560
ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg   4620
ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa   4680
aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg   4740
ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt   4800
gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg   4860
ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta   4920
atgcgccgct acagggcgcg tccattcgcc attcaggctg ctatggtgca ctctcagtac   4980
aatctgctct gatgccgcat agttaagcca gtaccagtca cgtagcgata tcggagtgta   5040
tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc   5100
gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc   5160
gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag   5220
cagatcaatt cgcgcgcgaa ggcgaagcgg catgcattta cgttgacacc atcgaatggt   5280
gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga   5340
atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg   5400
tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag   5460
cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac   5520
agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg   5580
tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag   5640
aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca   5700
gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct   5760
gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta   5820
ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc   5880
agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg   5940
gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact   6000
ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca   6060
ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt   6120
ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct   6180
catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca   6240
gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc   6300
ccgtctcact ggtgaaaaga aaaaccaccc tggcacccaa tacgcaaacc gcctctcccc   6360
gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc   6420
agtgagcgca acgcaattaa tgtgagttag cgcgaattga tctg                    6464
<210>  39
<211>  5584
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pDONR207
<400>  39
gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc     60
ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg    120
agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata    180
aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct    240
acaaactctt cctggctagc ggtaatacgg ttatccacag aatcagggga taacgcagga    300
aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg    360
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    420
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    480
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    540
ggaagcgtgg cgctttctca tagdtcacgc tgtaggtatc tcagttcggt gtaggtcgtt    600
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    660
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    720
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    780
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    840
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    900
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    960
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   1020
ttggtcatga gcttgcgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc   1080
aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca   1140
tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact   1200
caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc   1260
caacatcaat acaacctatt agtagccaac cactagaact atagctagag tcctgggcga   1320
acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca   1380
ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg   1440
tgcacagcac cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga   1500
ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca   1560
aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag ttgacataag   1620
cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa   1680
ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact   1740
gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc   1800
gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag   1860
ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg   1920
ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc   1980
ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc   2040
cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc   2100
gcggcttacg ttctgcccag gtttgagcag ccgcgtagtg agatctatat ctatgatctc   2160
gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag   2220
catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat   2280
cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc   2340
gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc ggcctaattt   2400
cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg   2460
tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg   2520
ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc   2580
gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg   2640
gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa   2700
tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt   2760
acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac   2820
catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg   2880
cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg   2940
agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt   3000
ttcccgttga atatggctca taacaccccc tgtattactg tttatgtaag cagacagttt   3060
tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac   3120
gggccagagc tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt   3180
cgttgcaaca aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg   3240
aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac   3300
agactacata atactgtaaa acacaacata tccagtcact atgaatcaac tacttagatg   3360
gtattagtga cctgtagtcg actaagttgg cagcatcacc cgacgcactt tgcgccgaat   3420
aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata   3480
ccgggaagcc ctgggccaac tttggcgaaa atgagacgtt gatcggcacg taagaggttc   3540
caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt   3600
ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat   3660
atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta   3720
taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca   3780
caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt   3840
ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac   3900
cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt   3960
ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta   4020
tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt   4080
caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat   4140
gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca   4200
tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga   4260
tgagtggcag ggcggggcgt aatcgcgtgg atccggctta ctaaaagcca gataacagta   4320
tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag   4380
tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc   4440
tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc   4500
agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg   4560
ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt   4620
gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat   4680
gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca   4740
cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa   4800
agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa   4860
gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg   4920
ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgata cagtagaaat   4980
tacagaaact ttatcacgtt tagtaagtat agaggctgaa aatccagatg aagccgaacg   5040
acttgtaaga gaaaagtata agagttgtga aattgttctt gatgcagatg attttcagga   5100
ctatgacact agcgtatatg aataggtaga tgtttttatt ttgtcacaca aaaaagaggc   5160
tcgcacctct ttttcttatt tctttttatg atttaatacg gcattgagga caatagcgag   5220
taggctggat acgacgattc cgtttgagaa gaacatttgg aaggctgtcg gtcgactaag   5280
ttggcagcat cacccgaaga acatttggaa ggctgtcggt cgactacagg tcactaatac   5340
catctaagta gttgattcat agtgactgga tatgttgtgt tttacagtat tatgtagtct   5400
gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt   5460
cagctttttt gtacaaagtt ggcattataa aaaagcattg ctcatcaatt tgttgcaacg   5520
aacaggtcac tatcagtcaa aataaaatca ttatttgggg cccgagatcc atgctagcgt   5580
taac                                                                5584
<210>  40
<211>  4470
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pDONR201
<220>
<221>  gene
<222>  (29)..(260)
<223>  attP1
<220>
<221>  gene
<222>  (656)..(961)
<223>  ccdB
<220>
<221>  gene
<222>  (1099)..(1184)
<223>  ccdA
<220>
<221>  gene
<222>  (1303)..(1962)
<223>  cmr
<220>
<221>  gene
<222>  (2210)..(2442)
<223>  attP2
<220>
<221>  gene
<222>  (2565)..(3374)
<223>  Kmr
<220>
<221>  gene
<222>  (3495)..(4134)
<223>  ori
<400>  40
gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc     60
tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa    120
gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa    180
aaacagacta cataatactg taaaacacaa catatccagt cactatgaat caactactta    240
gatggtatta gtgacctgta gtcgaccgac agccttccaa atgttcttcg ggtgatgctg    300
ccaacttagt cgaccgacag ccttccaaat gttcttctca aacggaatcg tcgtatccag    360
cctactcgct attgtcctca atgccgtatt aaatcataaa aagaaataag aaaaagaggt    420
gcgagcctct tttttgtgtg acaaaataaa aacatctacc tattcatata cgctagtgtc    480
atagtcctga aaatcatctg catcaagaac aatttcacaa ctcttatact tttctcttac    540
aagtcgttcg gcttcatctg gattttcagc ctctatactt actaaacgtg ataaagtttc    600
tgtaatttct actgtatcga cctgcagact ggctgtgtat aagggagcct gacatttata    660
ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag    720
ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc    780
agctttcatc cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca    840
gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct    900
gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca    960
tttcaccagt ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc   1020
tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca   1080
ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact   1140
gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcacac ctctttttga   1200
catacttcgg gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata   1260
cgcatactgt tatctggctt ttagtaagcc ggatccacgc gattacgccc cgccctgcca   1320
ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac   1380
ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt   1440
gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact   1500
ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg   1560
gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg   1620
ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa   1680
aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat   1740
acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa   1800
cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg   1860
gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg   1920
ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc   1980
tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa   2040
gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt   2100
cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg   2160
tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttagtcgact acaggtcact   2220
aataccatct aagtagttga ttcatagtga ctggatatgt tgtgttttac agtattatgt   2280
agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc   2340
tcgttcagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg   2400
caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct   2460
ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa   2520
caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac   2580
gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat   2640
gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg   2700
atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg   2760
agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta   2820
tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc   2880
aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc   2940
tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc   3000
gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg   3060
acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat   3120
tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg   3180
aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg   3240
atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt   3300
ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg   3360
atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga   3420
cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg   3480
agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt   3540
aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca   3600
agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac   3660
tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac   3720
atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct   3780
taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg   3840
gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca   3900
gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt   3960
aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta   4020
tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc   4080
gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc   4140
cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa   4200
ccgtattacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg   4260
gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg   4320
ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg   4380
ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt   4440
atttgatgcc tggcagttcc ctactctcgc                                    4470
<210>  41
<211>  4428
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pDONR212
<220>
<221>  gene
<222>  (866)...(1097)
<223>  attP1
<220>
<221>  gene
<222>  (1493)...(1798)
<223>  ccdB
<220>
<221>  gene
<222>  (2140)...(2799)
<223>  cmr
<220>
<221>  gene
<222>  (3047)...(3279)
<223>  attP2
<220>
<221>  gene
<222>  (3398)...(4128)
<223>  Km
<220>
<221>  gene
<222>  (4209)...(4229)
<223>  Tet0P
<400>  41
cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca     60
cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc    120
tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga    180
taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac    240
gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga    300
agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcaccag    360
ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg    420
acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag    480
caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc    540
tgcgttatcc cctgattctg tggataaccg tattaccgct agccaggaag agtttgtaga    600
aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat    660
ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc    720
ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc    780
cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt    840
aacgctagca tggatctcgg gccccaaata atgattttat tttgactgat agtgacctgt    900
tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagct    960
gatatcgaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa   1020
cagactacat aatactgtaa aacacaacat atccagtcac tatgaatcaa ctacttagat   1080
ggtattagtg acctgtagtc gaccgacagc cttccaaatg ttcttcgggt gatgctgcca   1140
acttagtcga ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg tatccagcct   1200
actcgctatt gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa aagaggtgcg   1260
agcctctttt ttgtgtgaca aaataaaaac atctacctat tcatatacgc tagtgtcata   1320
gtcctgaaaa tcatctgcat caagaacaat ttcacaactc ttatactttt ctcttacaag   1380
tcgttcggct tcatctggat tttcagcctc tatacttact aaacgtgata aagtttctgt   1440
aatttctact gtatcgacct gcagactggc tgtgtataag ggagcctgac atttatattc   1500
cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca   1560
cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc   1620
tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac   1680
gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta   1740
catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt   1800
caccagtccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca   1860
gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc   1920
tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat   1980
agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcacacctc tttttgacat   2040
acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc   2100
atactgttat ctggctttta gtaagccgga tccacgcgat tacgccccgc cctgccactc   2160
atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc   2220
atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc   2280
catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt   2340
gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa   2400
ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg   2460
gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac   2520
ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg   2580
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt   2640
gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt   2700
ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga   2760
tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga   2820
aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt   2880
ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc   2940
ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat   3000
ttattcggcg caaagtgcgt cgggtgatgc tgccaactta gtcgactaca ggtcactaat   3060
accatctaag tagttgattc atagtgactg gatatgttgt gttttacagt attatgtagt   3120
ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg   3180
ttcagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa   3240
cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc   3300
ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgttaga   3360
aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat   3420
atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga   3480
tggcaagatc ctgctatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta   3540
atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat   3600
ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat   3660
tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct   3720
gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca   3780
accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt   3840
ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag   3900
gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc   3960
tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact   4020
ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat   4080
cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg   4140
agcaagacgt ttcccgttga atatggctca tagatctttt ctccatcact gatagggagt   4200
ggtaaaataa ctccatcaat gatagagtgt caacaacatg accaaaatcc cttaacgtga   4260
gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc   4320
tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt   4380
ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactgg                4428
<210>  42
<211>  4627
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pDONR212(F)
<220>
<221>  gene
<222>  (17)...(248)
<223>  attP1
<220>
<221>  gene
<222>  (644)...(949)
<223>  ccdB
<220>
<221>  gene
<222>  (1291)...(1950)
<223>  cmr
<220>
<221>  gene
<222>  (2198)...(2430)
<223>  attP2
<220>
<221>  gene
<222>  (2549)...(3279)
<223>  Km
<220>
<221>  gene
<222>  (3360)...(3380)
<223>  Tet0P
<220>
<221>  gene
<222>  (3445)...(4084)
<223>  pUC ori
<400>  42
atggatctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa     60
caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc tgatatcgaa    120
acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca    180
taatactgta aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt    240
gacctgtagt cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg    300
accgacagcc ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat    360
tgtcctcaat gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt    420
tttgtgtgac aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa    480
atcatctgca tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc    540
ttcatctgga ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac    600
tgtatcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca    660
tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc    720
cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc    780
cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg    840
ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa    900
acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagtcc    960
ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct   1020
tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt   1080
gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc   1140
tgtcaactgt cactgtaata cgctgcttca tagcacacct ctttttgaca tacttcgggt   1200
atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta   1260
tctggctttt agtaagccgg atccacgcga ttacgccccg ccctgccact catcgcagta   1320
ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac   1380
ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa   1440
aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac   1500
ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag   1560
gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc   1620
gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca   1680
agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg   1740
atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt   1800
tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca   1860
ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac   1920
ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga   1980
taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct   2040
tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac   2100
agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc   2160
gcaaagtgcg tcgggtgatg ctgccaactt agtcgactac aggtcactaa taccatctaa   2220
gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt   2280
atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt   2340
cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt   2400
cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc   2460
aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgttag aaaaactcat   2520
cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa   2580
aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat   2640
cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct   2700
cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga   2760
atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt   2820
catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac   2880
gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca   2940
ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct   3000
ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga   3060
taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct   3120
catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat   3180
cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc   3240
atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg   3300
tttcccgttg aatatggctc atagatcttt tctccatcac tgatagggag tggtaaaata   3360
actccatcaa tgatagagtg tcaacaacat gaccaaaatc ccttaacgtg agttacgcgt   3420
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt   3480
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt   3540
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga   3600
taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag   3660
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata   3720
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg   3780
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga   3840
gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca   3900
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa   3960
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt   4020
tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac   4080
ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt   4140
ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga   4200
ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc   4260
tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag   4320
cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa   4380
cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg   4440
cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg   4500
cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca   4560
gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa   4620
cgctagc                                                             4627
<210>  43
<211>  4627
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pDONR212(R)
<220>
<221>  gene
<222>  (17)...(248)
<223>  attP1
<220>
<221>  gene
<222>  (644)...(949)
<223>  ccdB
<220>
<221>  gene
<222>  (1291)...(1950)
<223>  cmr
<220>
<221>  gene
<222>  (2198)...(2430)
<223>  attP2
<220>
<221>  gene
<222>  (2549)...(3279)
<223>  Km
<220>
<221>  gene
<222>  (3360)...(3380)
<223>  Tet0P
<220>
<221>  gene
<222>  (3683)...(4322)
<223>  pUC ori
<400>  43
atggatctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa     60
caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc tgatatcgaa    120
acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca    180
taatactgta aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt    240
gacctgtagt cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg    300
accgacagcc ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat    360
tgtcctcaat gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt    420
tttgtgtgac aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa    480
atcatctgca tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc    540
ttcatctgga ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac    600
tgtatcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca    660
tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc    720
cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc    780
cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg    840
ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa    900
acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagtcc    960
ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct   1020
tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt   1080
gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc   1140
tgtcaactgt cactgtaata cgctgcttca tagcacacct ctttttgaca tacttcgggt   1200
atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta   1260
tctggctttt agtaagccgg atccacgcga ttacgccccg ccctgccact catcgcagta   1320
ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac   1380
ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa   1440
aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac   1500
ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag   1560
gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc   1620
gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca   1680
agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg   1740
atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt   1800
tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca   1860
ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac   1920
ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga   1980
taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct   2040
tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac   2100
agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc   2160
gcaaagtgcg tcgggtgatg ctgccaactt agtcgactac aggtcactaa taccatctaa   2220
gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt   2280
atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt   2340
cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt   2400
cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc   2460
aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgttag aaaaactcat   2520
cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa   2580
aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat   2640
cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct   2700
cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga   2760
atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt   2820
catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac   2880
gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca   2940
ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct   3000
ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga   3060
taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct   3120
catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat   3180
cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc   3240
atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg   3300
tttcccgttg aatatggctc atagatcttt tctccatcac tgatagggag tggtaaaata   3360
actccatcaa tgatagagtg tcaacaacat gaccaaaatc ccttaacgtg agttacgcgt   3420
attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca   3480
ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc   3540
ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc   3600
aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc   3660
aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag   3720
gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc   3780
gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt   3840
tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct   3900
ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg   3960
ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct   4020
tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat   4080
tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg   4140
ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa   4200
aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt   4260
ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc   4320
tacggggtct gacgctcagt ggaacgacgc gtaccgctag ccaggaagag tttgtagaaa   4380
cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg   4440
cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg   4500
cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca   4560
gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa   4620
cgctagc                                                             4627
<210>  44
<211>  4396
<212>  DNA
<213>  Artificial sequence
<220>
<223>  attB cloning vector pCMVSPORT6.0
<400>  44
ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct     60
attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag    120
gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca    180
gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat    240
aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg    300
ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta    360
tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct    420
aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact    480
cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta    540
aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca    600
caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac    660
gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg accactttgt    720
acaagaaagc tgggtacgcg taagcttggg cccctcgagg gatcctctag agcggccgcc    780
gactagtgag ctcgtcgacg atatcccggg aattccggac cggtaccagc ctgctttttt    840
gtacaaactt gttctatagt gtcacctaaa taggcctaat ggtcatagct gtttcctgtg    900
tgaaattgtt atccgctccg cggcctaggc tagagtccgg aggctggatc ggtcccggtg    960
tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa   1020
acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg   1080
ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc   1140
attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc   1200
attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta   1260
ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt   1320
accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa   1380
gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt   1440
tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc   1500
aggcgggcca tttaccgtaa gttatgtaac gacatgcatc taatgagtga aagggcctcg   1560
tactacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg   1620
gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa   1680
atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaacgc   1740
gcgaattgca agctctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta   1800
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc   1860
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg   1920
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt   1980
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa   2040
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct   2100
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc   2160
cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg   2220
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct   2280
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag   2340
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga   2400
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga   2460
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg   2520
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag   2580
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag   2640
ggattttggt catgccataa cttcgtatag catacattat acgaagttat ggcatgagat   2700
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct   2760
aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta   2820
tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa   2880
ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac   2940
gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa   3000
gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag   3060
taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg   3120
tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag   3180
ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg   3240
tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc   3300
ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat   3360
tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata   3420
ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa   3480
aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca   3540
actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc   3600
aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc   3660
tttttcaata ttattgaagc atttatcagg gttattgtct catgccaggg gtgggcacac   3720
atatttgata ccagcgatcc ctacacagca cataattcaa tgcgacttcc ctctatcgca   3780
catcttagac ctttattctc cctccagcac acatcgaagc tgccgagcaa gccgttctca   3840
ccagtccaag acctggcatg agcggataca tatttgaatg tatttagaaa aataaacaaa   3900
taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt   3960
tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa   4020
tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag   4080
tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg   4140
tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga   4200
ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg   4260
gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg   4320
cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc   4380
cgctacaggg cgcgtc                                                   4396
<210>  45
<211>  15
<212>  DNA
<213>  Artificial sequence
<220>
<223>  Fis binding site 
<220> 
<221>  misc_feature
<222>  (2)..(2)
<223>  n can be any nucleotide
<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  w can be nucleotide a or t
<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  r can be nucleotide a or g
<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n can be any nucleotide
<400>  45
gntyaawwwt tranc                                                      15
<210>  46
<211>  20
<212>  DNA
<213>  Artificial sequence
<220>
<223>  Fis binding site
<400>  46
agtctgtttt ttatgcaaaa                                                 20
<210>  47
<211>  15
<212>  DNA
<213>  Artificial sequence
<220>
<223>  att core region
<400>  47
gcttttttat actaa                                                      15
<210>  48
<211>  21
<212>  DNA
<213>  Artificial sequence
<220>
<223>  att functional core region
<400>  48
caactttttt atacaaagtt g                                               21
<210>  49
<211>  98
<212>  PRT
<213>  Escherichia coli
<220>
<223>  Fis protein 
<400>  49
Met Phe Glu Gln Arg Val Asn Ser Asp Val Leu Thr Val Ser Thr Val 
1               5                   10                  15      
Asn Ser Gln Asp Gln Val Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Gln Ala Leu Lys Asn Tyr Phe Ala Gln Leu Asn Gly Gln Asp Val Asn 
35                  40                  45              
Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Met Val Met Ala Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Leu Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr Gly 
85                  90                  95      
Met Asn 
<210>  50
<211>  93
<212>  PRT
<213>  Klebsiella pneumoniae
<220>
<223>  Fis protein
<400>  50
Met Phe Glu Gln Arg Val Asn Ser Asp Val Leu Thr Val Ser Thr Val 
1               5                   10                  15      
Asn Ser Gln Asp Gln Val Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Gln Ala Leu Lys Asn Tyr Phe Ala Gln Leu Asn Gly Gln Asp Val Asn 
35                  40                  45              
Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Met Val Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Leu Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys 
85                  90              
<210>  51
<211>  98
<212>  PRT
<213>  Vibrio cholera
<220>
<223>  Fis protein
<400>  51
Met Phe Glu Gln Asn Leu Thr Ser Glu Ala Leu Thr Val Thr Thr Val 
1               5                   10                  15      
Thr Ser Gln Asp Gln Ile Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Ala Ser Leu Lys Asn Tyr Leu Ala Gln Leu Asn Gly Gln Glu Val Thr 
35                  40                  45              
Glu Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Thr Ile Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Thr Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr Gly 
85                  90                  95      
Met Asn 
<210>  52
<211>  99
<212>  PRT
<213>  Haemophilus influenzae
<220>
<223>  Fis protein
<400>  52
Met Leu Glu Gln Gln Arg Asn Ser Ala Asp Ala Leu Thr Val Ser Val 
1               5                   10                  15      
Leu Asn Ala Gln Ser Gln Val Thr Ser Lys Pro Leu Arg Asp Ser Val 
20                  25                  30          
Lys Gln Ala Leu Arg Asn Tyr Leu Ala Gln Leu Asp Gly Gln Asp Val 
35                  40                  45              
Asn Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu His Pro Met Leu 
50                  55                  60                  
Asp Met Ile Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Asn 
65                  70                  75                  80  
Met Leu Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr 
85                  90                  95      
Gly Met Gly 
<210>  53
<211>  107
<212>  PRT
<213>  Pseudomonas aeruginosa
<220>
<223>  Fis protein
<400>  53
Met Thr Thr Met Thr Thr Glu Thr Leu Val Ser Gly Thr Thr Pro Val 
1               5                   10                  15      
Ser Asp Asn Ala Asn Leu Lys Gln His Leu Thr Thr Pro Thr Gln Glu 
20                  25                  30          
Gly Gln Thr Leu Arg Asp Ser Val Glu Lys Ala Leu His Asn Tyr Phe 
35                  40                  45              
Ala His Leu Glu Gly Gln Pro Val Thr Asp Val Tyr Asn Met Val Leu 
50                  55                  60                  
Cys Glu Val Glu Ala Pro Leu Leu Glu Thr Val Met Asn His Val Lys 
65                  70                  75                  80  
Gly Asn Gln Thr Lys Ala Ser Glu Leu Leu Gly Leu Asn Arg Gly Thr 
85                  90                  95      
Leu Arg Lys Lys Leu Lys Gln Tyr Asp Leu Leu 
100                 105         
<210>  54
<211>  98
<212>  PRT
<213>  Salmonella typhimurium
<220>
<223>  Fis protein 
<400>  54
Met Phe Glu Gln Arg Val Asn Ser Asp Val Leu Thr Val Ser Thr Val 
1               5                   10                  15      
Asn Ser Gln Asp Gln Val Thr Gln Lys Pro Leu Arg Asp Ser Val Lys 
20                  25                  30          
Gln Ala Leu Lys Asn Tyr Phe Ala Gln Leu Asn Gly Gln Asp Val Asn 
35                  40                  45              
Asp Leu Tyr Glu Leu Val Leu Ala Glu Val Glu Gln Pro Leu Leu Asp 
50                  55                  60                  
Met Val Met Gln Tyr Thr Arg Gly Asn Gln Thr Arg Ala Ala Leu Met 
65                  70                  75                  80  
Met Gly Ile Asn Arg Gly Thr Leu Arg Lys Lys Leu Lys Lys Tyr Gly 
85                  90                  95      
Met Asn 
<210>  55
<211>  37
<212>  DNA
<213>  Artificial sequence
<220>
<223>  DE09 oligonucleotide
<400>  55
gggggctgca ggcaagaaga caaaaatcac cttgcgc                              37
<210>  56
<211>  37
<212>  DNA
<213>  Artificial sequence
<220>
<223>  DE10 oligonucleotide
<400>  56
gggggcccgg gcagaggcag ggagtgggac aaaattg                              37
<210>  57
<211>  51
<212>  DNA
<213>  Artificial sequence
<220>
<223>  DE46 oligonucleotide
<400>  57
ggagggaatt caggaggtat aaattaatgt tcgaacaacg cgtaaattct g              51
<210>  58
<211>  29
<212>  DNA
<213>  Artificial sequence
<220>
<223>  DE49 oligonucleotide
<400>  58
ggaggggatc cttattagtt catgccgta                                       29
<210>  59
<211>  48
<212>  DNA
<213>  Artificial sequence
<220>
<223>  DE162 oligonucleotide
<400>  59
ggaaggagat cttgctcaaa atttgagcta cataatactg taaaacac                  48
<210>  60
<211>  113
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pENTR1A
<400>  60
actttgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat ccggtaccga     60
attcgaattc gcggccgcac tcgagatatc tagacccagc tttcttgtac aaa           113
<210>  61
<211>  21
<212>  PRT
<213>  Unknown
<220>  
<223>  vector pENTR1A
<400>  61
Thr Leu Tyr Lys Lys Ala Gly Phe Lys Gly Thr Asn Ser Val Asp Trp 
1 5 10 15 
Ile Arg Tyr Arg Ile 
20
<210>  62
<211>  120
<212>  DNA
<213>  Artificial sequence
<220>
<223>  plasmid pDEST1
<400>  62
atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg agcggataac     60
aatttcacac aggaaacaga caggtatagg atcacaagtt tgtacaaaaa agctgaacga    120
<210>  63
<211>  405
<212>  DNA
<213>  Artificial sequence
<220>
<223>  attB cloning vector pCMVSPORT6.0
<400>  63
agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc     60
catagaagac accgggaccg atccagcctc cggactctag cctaggccgc ggagcggata    120
acaatttcac acaggaaaca gctatgacca ttaggcctat ttaggtgaca ctatagaaca    180
agtttgtaca aaaaagcagg ctggtaccgg tccggaattc ccgggatatc gtcgacgacg    240
tcactagtcg gcggccgctc tagagtatcc ctcgaggggc ccaagcttac gcgtacccag    300
ctttcttgta caaagtggtc cctatagtga gtcgtattgt aagctaggca ctggccgtcg    360
ttttacaacg tcgtgactgg gaaaactgct agcttgggat ctttg                    405