【手続補正書】
【提出日】平成17年2月9日(2005.2.9)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０２８７
【補正方法】変更
【補正の内容】
【０２８７】
【図１】34個の原発乳癌試料における免疫組織化学的検出および蛍光インサイチューハイブリダイゼーション検出の結果を示す。
【図２】典型的トポIIαプローブの3'末端(配列番号：9)、および典型的トポIIαプローブの5'末端(配列番号：10)を示す。
【図３】トポIIαプローブおよび染色体17番プローブを使用するISH結果を解釈するために有用なチャートを示す。
【図４】ABL遺伝子に隣接している実施例14に使用されるBACクローンを示す。
【図５】ABL転座、関与するパートナー遺伝子およびABL転座をもつ白血病を示す。
【図６】図6Aは、ABL DNAの模式図を示し、図6Bは、ABL遺伝子における様々な区切り点を示す。
【図７】BCR-ABL転座を示す。
【図８】示されている区切り点をもつBCR遺伝子およびABL遺伝子の簡略化模式図を、BCRおよびABLの遺伝子上での個々の切断から生じる典型的BCR/ABL転写物およびタンパク質と共に示す。
【図９】最もよくあるBCR-ABL融合体の臨床病理的な相関物を示す。
【図１０】ABL遺伝子についてのUCSCゲノムブラウザーを示す。
【図１１】二色インサイチューハイブリダイゼーション(例えば、CISHまたはFISH)によるABL転座検出の概略図を示す。黒点は、ABL.cを表し、白点は、ABL.tを表す。部分的核型および対応する間期核が図に示されている。ABL転座のない正常な細胞は、並置の黒点と白点を示し、一方、ABL転座をもつ細胞は、分離された黒点と白点の1対を示す。ABL転座およびABL遺伝子区切り点に対してセントロメアである染色体物質の欠失をもつ細胞は、並置の黒点と白点の1対を示し、もう一つの対の黒点は消えている(欠失している)。
【配列表】
SEQUENCE LISTING
<110>  ZYMED LABORATORIES, INC.
<120>  Chromogenic In Situ Hybridization Methods, Kits, and Compositions
<130>  ME-A0403
<140>  JP   2003-529901
<141>  2002-09-13
<150>  US   09/952,851
<151>  2001-09-14
<150>  US   10/173,525
<151>  2002-06-17
<160>  31
<170>  PatentIn version 3.1
<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  1
gcctccctaa cctgattggt tt                                              22
<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  2
ctgaagaacc ctgaaagcga ct                                              22
<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  3
ctggctccga tgtatttgat g                                               21
<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  4
cctgcccata agtctctctg ct                                              22
<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  5
gattagcctg ccctctttgg                                                 20
<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  6
cagaagggag gcagacagtc                                                 20
<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  7
gctcatggtg tcaggaggat g                                               21
<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  8
gcaggaatag gtgggatgga g                                               21
<210>  9
<211>  406
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  The n at this position can be a, c, t, or g.
<400>  9
gaagatacat ccaaantcca gcctacgcaa cagagcagga ttcagtctca aaaaaagaaa     60
aaaagaaaag aaaacgttcc ccaccccatc tccttccttg atcatcactg gaccctgttc    120
tgccaccaac ttgcgtgaac ttggagtttg actgacctta gctgtaacat ggaggtagat    180
catctccacc catcctacct cttgaagctc ttgtgagagt aaaatgaatg gagaagagta    240
gttctgctcc caatgccaga catgtgccct gttcagcaag cccaagagga gaaaaggtgc    300
caggacacag aggcaggagt gcaggagagg ccggacaaac ccacgcaaca tgcctgggat    360
gaagcatgag tgcaggtgag tgtgggaatc tgcaaaggtt gccaga                   406
<210>  10
<211>  750
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (52)..(52)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (64)..(65)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (85)..(85)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (87)..(87)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (96)..(96)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (108)..(108)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (112)..(112)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (122)..(122)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (131)..(131)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (133)..(133)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (165)..(165)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (178)..(178)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (181)..(182)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (199)..(199)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (210)..(210)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (218)..(218)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (231)..(231)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (287)..(287)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (297)..(297)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (312)..(312)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (321)..(321)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (323)..(323)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (334)..(334)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (338)..(338)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (368)..(368)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (414)..(414)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (416)..(416)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (434)..(434)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (436)..(436)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (443)..(443)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (447)..(447)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (451)..(451)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (461)..(461)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (467)..(467)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (479)..(479)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (481)..(481)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (489)..(489)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (508)..(508)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (530)..(530)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (533)..(533)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (554)..(554)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (557)..(558)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (574)..(574)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (578)..(578)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (585)..(585)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (587)..(587)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (590)..(590)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (596)..(596)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (600)..(600)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (602)..(602)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (605)..(605)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (607)..(607)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (619)..(622)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (632)..(632)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (646)..(646)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (649)..(649)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (653)..(653)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (658)..(658)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (663)..(663)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (666)..(666)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (669)..(669)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (677)..(677)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (682)..(682)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (690)..(690)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (711)..(711)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (715)..(715)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (718)..(718)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (721)..(721)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (724)..(724)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (728)..(728)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (731)..(731)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (739)..(739)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (743)..(743)
<223>  The n at this position can be a, c, t, or g.
<220>
<221>  misc_feature
<222>  (745)..(745)
<223>  The n at this position can be a, c, t, or g.
<400>  10
gcngnngnca agcctnccaa ggtaggnttc cgannggcgg ccgcctggcc gncnacattt     60
aagnngacac tatagaagga tcgtngnatt gttgcntccc tctttacngg cnctatggct    120
cnattttgtt ngntactgag gggtaaaaga taaatgttta ccntnaccta aaattggntt    180
nnggcctcta aaggaaccng aggcttaaan gaattatngg ctttggaagc nggccttcaa    240
attactgcgc taatttatat ttttcattaa aaactcagct ggcctcntct atatagntgt    300
cttccctggc cntgaaaccc nantgtttcg ccanaaanga ttttaaaatt aaggggtcat    360
aattcccncc ccatgatgtg tggattaatg gtaagaagga tgcccagaac gttntnttct    420
taggttgaac gaananaaaa gtnaaanagt ngggctctgg nttctcncct ttgaagccnc    480
ncaattcgng agatactatg ctgaaccnta gtttttcttt atataggggn gtngaacttt    540
accctcaaaa tcantanntc agcacatcaa gganattntg gatcntnggn tcttcnctgn    600
cnccnanatg ctgggaccnn nnaccttgca tnaacagttt gctttngtnc ctntgcanag    660
ggntgngcnt ttccaanagg gnaaggcaan ggcctaacat catacctggg ngccnagnaa    720
nccnaaanac ngggaaggnc tcncntaccc                                     750
<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  11
gcctccctaa cctgattggt ta                                              22
<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  12
ctcaagaacc ctgaaagcga ct                                              22
<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  13
gctacggtct gctcaggaca gtt                                             23
<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  14
ctgtcctgag cataccgtag c                                               21
<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  15
aattcttgcg ccttaaacca ac                                              22
<210>  16
<211>  18
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  16
gttggtttaa ggcgcaag                                                   18
<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  17
aattcttgcg ccttaaacca ac                                              22
<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  18
ctgagcggaa ttcgtgagac c                                               21
<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  19
ggtctcacga attccgctca gtt                                             23
<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  20
accgtaggac tctgctggcg att                                             23
<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  21
tcgccagcag agtcctacgg t                                               21
<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  22
atcggtgtag cctgaatgga ctt                                             23
<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  23
gtccattcag gctacaccga t                                               21
<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  24
catcattcgg tcagaggcac ttt                                             23
<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  25
agtgcctctg accgaatgat g                                               21
<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  26
tcttcacgac acgacagcca g                                               21
<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  27
ctggctgtcg tgtcgtgaag att                                             23
<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  28
atgcgtccac cttgacctta c                                               21
<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  29
gtaaggtcaa ggtggacgca ttt                                             23
<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  30
atagccccga atcaggtgga a                                               21
<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Synthetic
<400>  31
ttccacctga ttcggggcta ttt                                             23