【手続補正書】
【提出日】平成16年11月15日(2004.11.15)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】００２５
【補正方法】変更
【補正の内容】
【００２５】
本明細書に使用される「融合タンパク質」という用語は、外因性タンパク質断片に結合した関心対象のタンパク質を含むキメラタンパク質を意味する。融合相手は、宿主細胞で発現されるように、組換えキメラタンパク質の溶解性を増強させてもよく、宿主細胞もしくは培養上清またはその両方からの組換え融合タンパク質の精製を可能にするために、アフィニティータグ（例えばヒス-タグ）を付与してもよい。望ましい場合には、融合タンパク質は、当技術分野に公知の様々な酵素的手法または化学的手法によって、関心対象のタンパク質から分離してもよい。
【配列表】
SEQUENCE LISTING
<110>  THIRD WAVE TECHNOLOGIES, INC.
<120>  FEN Endonucleases
<130>  ME-A0307
<140>  JP   2002-570777
<141>  2001-11-15
<150>  US   09/714,935
<151>  2000-11-17
<150>  US   09/713,601
<151>  2000-11-15
<160>  2   
<170>  PatentIn version 3.1
<210>  1
<211>  338
<212>  PRT
<213>  Archaeoglobus veneficus
<400>  1
Met Gly Ala Asp Ile Gly Glu Leu Leu Glu Arg Glu Glu Val Glu Leu 
1               5                   10                  15      
Glu Tyr Phe Ser Gly Arg Lys Ile Ala Ile Asp Ala Phe Asn Thr Leu 
20                  25                  30          
Tyr Gln Phe Ile Ser Ile Ile Arg Gln Pro Asp Gly Thr Pro Leu Lys 
35                  40                  45              
Asp Ser Gln Gly Arg Met Thr Ser His Leu Ser Gly Ile Leu Tyr Arg 
50                  55                  60                  
Val Ser Asn Met Ile Glu Val Gly Met Arg Pro Ile Phe Val Phe Asp 
65                  70                  75                  80  
Gly Glu Pro Pro Val Phe Lys Gln Lys Glu Ile Glu Glu Arg Lys Glu 
85                  90                  95      
Arg Arg Ala Glu Ala Glu Glu Lys Trp Ile Ala Ala Ile Glu Arg Gly 
100                 105                 110         
Glu Lys Tyr Ala Lys Lys Tyr Ala Gln Ala Ala Ala Arg Val Asp Glu 
115                 120                 125             
Tyr Ile Val Glu Ser Ser Lys Lys Leu Leu Glu Tyr Met Gly Val Pro 
130                 135                 140                 
Trp Val Gln Ala Pro Ser Glu Gly Glu Ala Gln Ala Ala Tyr Met Ala 
145                 150                 155                 160 
Ala Lys Gly Asp Val Asp Phe Thr Gly Ser Gln Asp Tyr Asp Ser Leu 
165                 170                 175     
Leu Phe Gly Ser Pro Lys Leu Ala Arg Asn Leu Ala Ile Thr Gly Lys 
180                 185                 190         
Arg Lys Leu Pro Gly Lys Asn Val Tyr Val Glu Val Lys Pro Glu Ile 
195                 200                 205             
Ile Asp Leu Asn Gly Asn Leu Arg Arg Leu Gly Ile Thr Arg Glu Gln 
210                 215                 220                 
Leu Val Asp Ile Ala Leu Leu Val Gly Thr Asp Tyr Asn Glu Gly Val 
225                 230                 235                 240 
Lys Gly Val Gly Val Lys Lys Ala Tyr Lys Tyr Ile Lys Thr Tyr Gly 
245                 250                 255     
Asp Val Phe Lys Ala Leu Lys Ala Leu Lys Val Glu Gln Glu Asn Ile 
260                 265                 270         
Glu Glu Ile Arg Asn Phe Phe Leu Asn Pro Pro Val Thr Asn Asn Tyr 
275                 280                 285             
Ser Leu His Phe Gly Lys Pro Asp Asp Glu Lys Ile Ile Glu Phe Leu 
290                 295                 300                 
Cys Glu Glu His Asp Phe Ser Lys Asp Arg Val Glu Lys Ala Val Glu 
305                 310                 315                 320 
Lys Leu Lys Ala Gly Met Gln Ala Ser Gln Ser Thr Leu Glu Arg Trp 
325                 330                 335     
Phe Ser 
<210>  2
<211>  1017
<212>  DNA
<213>  Archaeoglobus veneficus
<400>  2
atgggtgcag acataggcga actactcgag agagaagaag ttgaacttga gtacttctcc     60
gggagaaaaa tagctattga tgcttttaac actctttacc agttcatatc tatcataagg    120
caacctgacg gcactccttt gaaggattct cagggtagaa tgacctcaca cctctccggc    180
atcctgtacc gcgtgtcaaa catgatcgag gttggaatga gacccatttt cgttttcgat    240
ggtgagcctc ctgttttcaa gcagaaggag atagaggaac gaaaggaaag aagagctgaa    300
gcagaggaga agtggatcgc tgcgatagag agaggagaga agtacgcaaa gaagtacgct    360
caggcagcgg cgagggttga tgaatacatc gtcgagtcgt caaagaagct gcttgagtat    420
atgggagttc catgggttca ggcgccgagt gagggagagg cacaggctgc atacatggca    480
gcgaagggcg atgtagattt tactggctcg caggattacg actcgcttct cttcggcagc    540
ccaaagcttg caagaaatct cgcgattact ggaaagagga agctgcccgg aaagaatgtt    600
tacgttgagg tcaaaccaga gataatagac ttaaacggca acctgagaag gcttggaata    660
acaagggaac agctcgtcga tatcgcgttg ctcgtgggaa cggactacaa cgaaggagtg    720
aagggcgttg gggtcaagaa ggcctacaag tacataaaaa cctacggaga tgttttcaaa    780
gctctcaagg ccttaaaggt agagcaggag aacatagagg agataagaaa cttcttcctg    840
aacccgcctg ttacgaacaa ctacagcctc cacttcggaa agccagacga tgagaagatt    900
atcgagttcc tgtgtgaaga gcacgacttt agcaaggata gggtagagaa ggccgttgag    960
aagctgaaag caggaatgca agcctcgcaa tcaacgcttg agaggtggtt ttcctga      1017