【手続補正書】
【提出日】平成17年1月26日(2005.1.26)
【手続補正１】
【補正対象書類名】明細書
【補正対象項目名】０１９７
【補正方法】変更
【補正の内容】
【０１９７】
参照
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【配列表】
SEQUENCE LISTING
<110>  Emory University
<120>  DNA EXPRESSION VECTORS AND METHODS OF USE
<130>  FR-A0216
<140>  JP 2002-500664
<141>  2001-03-02
<150>  US 60/186,364
<151>  2000-03-02
<150>  US 60/251,083
<151>  2000-12-01
<160>  24    
<170>  PatentIn version 3.0
<210>  1
<211>  3894
<212>  DNA
<213>  Artificial
<220>
<223>  Vaccine vector pGA1
<220>
<221>  promoter
<222>  (1)..(692)
<223>  cytomegalovirus intermediate early  promoter 
<220>
<221>  misc_feature
<222>  (693)..(1641)
<223>  cytomegalovirus Intron A
<220>
<221>  misc_feature
<222>  (1662)..(1724)
<223>  tissue plasmoinogen activator leader
<220>
<221>  misc_feature
<222>  (1764)..(1986)
<223>  bovine growth hormone polyadenylation signal
<220>
<221>  misc_feature
<222>  (1987)..(2021)
<223>  Lamda T0 terminator
<220>
<221>  misc_feature
<222>  (2040)..(2833)
<223>  kanamycin resistance gene
<220>
<221>  misc_feature
<222>  (2834)..(3894)
<223>  Col E1 origin of replication
<400>  1
cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta     60
tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata    120
gtaatcaatt acgggttcat tagttcatag cccatatatg gagttccgcg ttacataact    180
tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat    240
gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta    300
tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc    360
tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg    420
ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg    480
gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct    540
ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa    600
atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt    660
ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg    720
ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat    780
tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagac tctataggca    840
cacccctttg gctcttatgc atgctatact gtttttggct tggggcctat acacccccgc    900
ttccttatgc tataggtgat ggtatagctt agcctatagg tgtgggttat tgaccattat    960
tgaccactcc cctattggtg acgatacttt ccattactaa tccataacat ggctctttgc   1020
cacaactatc tctattggct atatgccaat actctgtcct tcagagactg acacggactc   1080
tgtattttta caggatgggg tcccatttat tatttacaaa ttcacatata caacaacgcc   1140
gtcccccgtg cccgcagttt ttattaaaca tagcgtggga tctccacgcg aatctcgggt   1200
acctgttccg gacatgggyt cttctccggt agcggcggag cttccacatc cgagccctgg   1260
tcccatgcct ccagcggctc atggtcgctc ggcagctcct tgctcctaac agtggaggcc   1320
agacttaggc acagcacaat gcccaccacc accagtgtgc cgcacaaggc cgtggcggta   1380
gggtatgtgt ctgaaaatga gctcggagat tgggctcgca ccgctgacgc agatggaaga   1440
cttaaggcag cggcagaaga agatgcaggc agctgagttg ttgtattctg ataagagtca   1500
gaggtaactc ccgttgcggt gctgttaacg gtggagggca gtgtagtctg agcagtactc   1560
gttgctgccg cgcgcgccac cagacataat agctgacaga ctaacagact gttcctttcc   1620
atgggtcttt tctgcagtca ccatcgatgc ttgcaatcat ggatgcaatg aagagagggc   1680
tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc ggctagcccc gggtgataaa   1740
cggaccgcgc aatccctagg ctgtgccttc tagttgccag ccatctgttg tttgcccctc   1800
ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga   1860
ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca   1920
ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc   1980
tatataaaaa acgcccggcg gcaaccgagc gttctgaacg ctagagtcga caaattcaga   2040
agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt   2100
aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag   2160
ccaacgctat gtcctgatag cggtctgcca cacccagccg gccacagtcg atgaatccag   2220
aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga   2280
gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc   2340
cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg   2400
ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat   2460
gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg   2520
acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga   2580
caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg   2640
cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc   2700
gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc   2760
agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt   2820
gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc   2880
atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac   2940
cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta   3000
gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc   3060
ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg   3120
ctttctacgt gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc   3180
cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt   3240
cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac   3300
cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct   3360
tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact   3420
tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg   3480
ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata   3540
aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga   3600
cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag   3660
ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg   3720
agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac   3780
ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca   3840
acgcggccct tttacggttc ctggcctttt gctggccttt tgctcacatg ttgt         3894
<210>  2
<211>  2947
<212>  DNA
<213>  Artificial
<220>
<223>  Vaccine vector pGA2
<220>
<221>  promoter
<222>  (1)..(682)
<223>  cytomegalovirus intermediate early promoter
<220>
<221>  misc_feature
<222>  (712)..(774)
<223>  tissue plasminogen activator leader
<220>
<221>  misc_feature
<222>  (814)..(1036)
<223>  bovine growth hormone polyadenylation signal
<220>
<221>  misc_feature
<222>  (1037)..(1071)
<223>  Lambda T0 promoter
<220>
<221>  misc_feature
<222>  (1090)..(1883)
<223>  kanamycin resistancgene
<220>
<221>  misc_feature
<222>  (1884)..(2947)
<223>  Col E1 origin of replication
<400>  2
cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta     60
tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata    120
gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact    180
tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat    240
gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta    300
tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc    360
tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg    420
ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg    480
gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct    540
ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa    600
atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt    660
ctatataagc agagctcgtt tagtgaactc attctatcga tgcttgcaat catggatgca    720
atgaagagag ggctctgctg tgtgctgctg ctgtgtggag cagtcttcgt ttcggctagc    780
cccgggtgat aaacggaccg cgcaatccct aggctgtgcc ttctagttgc cagccatctg    840
ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt    900
cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg    960
gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg   1020
atgcggtggg ctctatataa aaaacgcccg gcggcaaccg agcgttctga acgctagagt   1080
cgacaaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga   1140
gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca   1200
atatcacggg tagccaacgc tatgtcctga tagcggtctg ccacacccag ccggccacag   1260
tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca   1320
tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg   1380
gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc   1440
atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc   1500
ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga   1560
gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt   1620
cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac   1680
gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca   1740
aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt   1800
gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg   1860
tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt   1920
gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc   1980
ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa   2040
accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg   2100
cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt   2160
ttctgcggac tggctttcta cgtgaaaagg atctaggtga agatcctttt tgataatctc   2220
atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag   2280
atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa   2340
aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg   2400
aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag   2460
ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg   2520
ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga   2580
tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc   2640
ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc   2700
acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga   2760
gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt   2820
cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg   2880
aaaaacgcca gcaacgcggc ccttttacgg ttcctggcct tttgctggcc ttttgctcac   2940
atgttgt                                                             2947
<210>  3
<211>  3893
<212>  DNA
<213>  Artificial
<220>
<223>  Vaccine vector pGA3
<220>
<221>  promoter
<222>  (1)..(690)
<223>  cytomegalovirus intermediate early promoter
<220>
<221>  misc_feature
<222>  (691)..(1638)
<223>  cytomegalovirus intron A
<220>
<221>  misc_feature
<222>  (1659)..(1721)
<223>  tissue plasminogen activator leader
<220>
<221>  misc_feature
<222>  (1760)..(1982)
<223>  bovine growth hormone polyadenylation signal
<220>
<221>  misc_feature
<222>  (1983)..(2017)
<223>  Lamda T0 promoter
<220>
<221>  misc_feature
<222>  (2036)..(2829)
<223>  kanamycin resistance gene
<220>
<221>  misc_feature
<222>  (2830)..(3889)
<223>  ColE1 origin of replication
<400>  3
cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta     60
tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata    120
gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact    180
tacggtaaat ggcccgcctg gctgaccccc caacgacccc cgcccattga cgtcaataat    240
gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta    300
tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc    360
tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg    420
ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg    480
gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct    540
ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa    600
atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt    660
ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg    720
ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat    780
tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagac tctataggca    840
cacccctttg gctcttatgc atgctatact gtttttggct tggggcctat acacccccgc    900
ttccttatgc tataggtgat ggtatagctt agcctatagg tgtgggttat tgaccattat    960
tgaccactcc cctattggtg acgatacttt ccattactaa tccataacat ggctctttgc   1020
cacaactatc tctattggct atatgccaat actctgtcct tcagagactg acacggactc   1080
tgtattttta caggatgggg tcccatttat tatttacaaa ttcacatata caacaacgcc   1140
gtcccccgtg cccgcagttt ttattaaaca tagcgtggga tctccacgcg aatctcgggt   1200
acgtgttccg gacatgggct cttctccggt agcggcggag cttccacatc cgagccctgg   1260
tcccatgcct ccagcggctc atggtcgctc ggcagctcct tgctcctaac agtggaggcc   1320
agacttaggc acagcacaat gcccaccacc accagtgtgc cgcacaaggc cgtggcggta   1380
gggtatgtgt ctgaaaatga gctcggagat tgggctcgca ccgctgacgc agatggaaga   1440
cttaaggcag cggcagaaga agatgcaggc agctgagttg ttgtattctg ataagagtca   1500
gaggtaactc ccgttgcggt gctgttaacg gtggagggca gtgtagtctg agcagtactc   1560
gttgctgccg cgcgcgccac cagacataat agctgacaga ctaacagact gttcctttcc   1620
atgggtcttt tctgcagtca ccgtccaagc ttgcaatcat ggatgcaatg aagagagggc   1680
tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc ggctagcccc gggtgataag   1740
gatcctcgca atccctaggc tgtgccttct agttgccagc catctgttgt ttgcccctcc   1800
cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag   1860
gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag   1920
gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct   1980
atataaaaaa cgcccggcgg caaccgagcg ttctgaacgc tagagtcgac aaattcagaa   2040
gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta   2100
aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc   2160
caacgctatg tcctgatagc ggtctgccac acccagccgg ccacagtcga tgaatccaga   2220
aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag   2280
atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc   2340
ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc   2400
tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg   2460
cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga   2520
caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac   2580
aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc   2640
ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg   2700
cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca   2760
gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg   2820
ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca   2880
tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc   2940
agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag   3000
ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct   3060
tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc   3120
tttctacgtg aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc   3180
ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc   3240
ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc   3300
agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt   3360
cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt   3420
caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc   3480
tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa   3540
ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac   3600
ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg   3660
gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga   3720
gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact   3780
tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa   3840
cgcggccctt ttacggttcc tggccttttg ctggcctttt gctcacatgt tgt          3893
<210>  4
<211>  9544
<212>  DNA
<213>  Artificial
<220>
<223>  Construct of vaccine vector pGA2 and insert JS2 expressing clade 
HIV-1 VL
<220>
<221>  misc_feature
<222>  (106)..(1641)
<223>  encoding inner core protein
<220>
<221>  misc_feature
<222>  (1401)..(3617)
<223>  encoding viral rproteins including reverse transcriptase
<220>
<221>  misc-feature
<222>  (3708)..(6334)
<223>  induces high level of virus particles
<220>
<221>  misc-feature
<222>  (3847)..(6518)
<223>  encodes Rev regulating transfer of RNA to cytoplasm
<220>
<221>  misc_feature
<222>  (4102)..(6660)
<223>  encoding ADA Env 
<220>
<221>  misc_feature
<222>  (6672)..(9544)
<223>  Vaccine vector pGA2 
<400>  4
atcgatgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg     60
tgggtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg    120
tcagtattaa gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga    180
aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca    240
gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa    300
ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc    360
tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag    420
gaagagcaaa acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc    480
agtcaggtca gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag    540
gccatatcac ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc    600
ccagaagtaa tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac    660
accatgctaa acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc    720
aatgaggaag ctgcagaatg ggatagagta catccagtgc atgcagggcc tattgcacca    780
ggccagatga gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa    840
caaataggat ggatgacaaa taatccacct atcccagtag gagaaattta taaaagatgg    900
ataatcctgg gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata    960
agacaaggac caaaagaacc ttttagagac tatgtagacc ggttctataa aactctaaga   1020
gccgagcaag cttcacagga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat   1080
gcgaacccag attgtaagac tattttaaaa gcattgggac cagcggctac actagaagaa   1140
atgatgacag catgtcaggg agtaggagga cccggccata aggcaagagt tttggctgaa   1200
gcaatgagcc aagtaacaaa tacagctacc ataatgatgc agagaggcaa ttttaggaac   1260
caaagaaaga tggttaagag cttcaatagc ggcaaagaag ggcacacagc cagaaattgc   1320
agggccccta ggaaaaaggg cagctggaaa agcggaaagg aaggacacca aatgaaagat   1380
tgtactgaga gacaggctaa ttttttaggg aagatctggc cttcctacaa gggaaggcca   1440
gggaattttc ttcagagcag accagagcca acagccccac catttcttca gagcagacca   1500
gagccaacag ccccaccaga agagagcttc aggtctgggg tagagacaac aactccccct   1560
cagaagcagg agccgataga caaggaactg tatcctttaa cttccctcag atcactcttt   1620
ggcaacgacc cctcgtcaca ataaagatag gggggcaact aaaggaagct ctattagata   1680
caggagcaga tgatacagta ttagaagaaa tgagtttgcc aggaagatgg aaaccaaaaa   1740
tgataggggg aattggaggt tttatcaaag taagacagta tgatcagata ctcatagaaa   1800
tctgtggaca taaagctata ggtacagtat tagtaggacc tacacctgtc aacataattg   1860
gaagaaatct gttgactcag attggttgca ctttaaattt tcccattagc cctattgaga   1920
ctgtaccagt aaaattaaag ccaggaatgg atggcccaaa agttaaacaa tggccattga   1980
cagaagaaaa aataaaagca ttagtagaaa tttgtacaga aatggaaaag gaagggaaaa   2040
tttcaaaaat tgggcctgag aatccataca atactccagt atttgccata aagaaaaaag   2100
acagtactaa atggagaaaa ttagtagatt tcagagaact taataagaga actcaagact   2160
tctgggaagt tcaattagga ataccacatc ccgcagggtt aaaaaagaaa aaatcagtaa   2220
cagtactgga tgtgggtgat gcatattttt cagttccctt agatgaagac ttcaggaagt   2280
atactgcatt taccatacct agtataaaca atgagacacc agggattaga tatcagtaca   2340
atgtgcttcc acagggatgg aaaggatcac cagcaatatt ccaaagtagc atgacaaaaa   2400
tcttagagcc ttttaaaaaa caaaatccag acatagttat ctatcaatac atgaacgatt   2460
tgtatgtagg atctgactta gaaatagggc agcatagaac aaaaatagag gagctgagac   2520
aacatctgtt gaggtgggga cttaccacac cagacaaaaa acatcagaaa gaacctccat   2580
tcctttggat gggttatgaa ctccatcctg ataaatggac agtacagcct atagtgctgc   2640
cagaaaaaga cagctggact gtcaatgaca tacagaagtt agtggggaaa ttgaataccg   2700
caagtcagat ttacccaggg attaaagtaa ggcaattatg taaactcctt agaggaacca   2760
aagcactaac agaagtaata ccactaacag aagaagcaga gctagaactg gcagaaaaca   2820
gagagattct aaaagaacca gtacatggag tgtattatga cccatcaaaa gacttaatag   2880
cagaaataca gaagcagggg caaggccaat ggacatatca aatttatcaa gagccattta   2940
aaaatctgaa aacaggaaaa tatgcaagaa tgaggggtgc ccacactaat gatgtaaaac   3000
aattaacaga ggcagtgcaa aaaataacca cagaaagcat agtaatatgg ggaaagactc   3060
ctaaatttaa actacccata caaaaggaaa catgggaaac atggtggaca gagtattggc   3120
aagccacctg gattcctgag tgggagtttg ttaatacccc tcctttagtg aaattatggt   3180
accagttaga gaaagaaccc atagtaggag cagaaacctt ctatgtagat ggggcagcta   3240
acagggagac taaattagga aaagcaggat atgttactaa caaaggaaga caaaaggttg   3300
tccccctaac taacacaaca aatcagaaaa ctcagttaca agcaatttat ctagctttgc   3360
aggattcagg attagaagta aacatagtaa cagactcaca atatgcatta ggaatcattc   3420
aagcacaacc agataaaagt gaatcagagt tagtcaatca aataatagag cagttaataa   3480
aaaaggaaaa ggtctatctg gcatgggtac cagcacacaa aggaattgga ggaaatgaac   3540
aagtagataa attagtcagt gctggaatca ggaaaatact atttttagat ggaatagata   3600
aggcccaaga tgaacattag aattctgcaa caactgctgt ttatccattt tcagaattgg   3660
gtgtcgacat agcagaatag gcgttactcg acagaggaga gcaagaaatg gagccagtag   3720
atcctagact agagccctgg aagcatccag gaagtcagcc taaaactgct tgtaccaatt   3780
gctattgtaa aaagtgttgc tttcattgcc aagtttgttt cataacaaaa gccttaggca   3840
tctcctatgg caggaagaag cggagacagc gacgaagacc tcctcaagac agtcagactc   3900
atcaagtttc tctatcaaag cagtaagtag taaatgtaat gcaaccttta caaatattag   3960
caatagtagc attagtagta gcagcaataa tagcaatagt tgtgtggacc atagtattca   4020
tagaatatag gaaaatatta agacaaagaa aaatagacag gttaattgat aggataacag   4080
aaagagcaga agacagtggc aatgaaagtg aaggggatca ggaagaatta tcagcacttg   4140
tggaaatggg gcatcatgct ccttgggatg ttgatgatct gtagtgctgt agaaaatttg   4200
tgggtcacag tttattatgg ggtacctgtg tggaaagaag caaccaccac tctattttgt   4260
gcatcagatg ctaaagcata tgatacagag gtacataatg tttgggccac acatgcctgt   4320
gtacccacag accccaaccc acaagaagta gtattggaaa atgtgacaga aaattttaac   4380
atgtggaaaa ataacatggt agaacagatg catgaggata taatcagttt atgggatcaa   4440
agcctaaagc catgtgtaaa attaacccca ctctgtgtta ctttaaattg cactgatttg   4500
aggaatgtta ctaatatcaa taatagtagt gagggaatga gaggagaaat aaaaaactgc   4560
tctttcaata tcaccacaag cataagagat aaggtgaaga aagactatgc acttttttat   4620
agacttgatg tagtaccaat agataatgat aatactagct ataggttgat aaattgtaat   4680
acctcaacca ttacacaggc ctgtccaaag gtatcctttg agccaattcc catacattat   4740
tgtaccccgg ctggttttgc gattctaaag tgtaaagaca agaagttcaa tggaacaggg   4800
ccatgtaaaa atgtcagcac agtacaatgt acacatggaa ttaggccagt agtgtcaact   4860
caactgctgt taaatggcag tctagcagaa gaagaggtag taattagatc tagtaatttc   4920
acagacaatg caaaaaacat aatagtacag ttgaaagaat ctgtagaaat taattgtaca   4980
agacccaaca acaatacaag gaaaagtata catataggac caggaagagc attttataca   5040
acaggagaaa taataggaga tataagacaa gcacattgca acattagtag aacaaaatgg   5100
aataacactt taaatcaaat agctacaaaa ttaaaagaac aatttgggaa taataaaaca   5160
atagtcttta atcaatcctc aggaggggac ccagaaattg taatgcacag ttttaattgt   5220
ggaggggaat ttttctactg taattcaaca caactgttta atagtacttg gaattttaat   5280
ggtacttgga atttaacaca atcgaatggt actgaaggaa atgacactat cacactccca   5340
tgtagaataa aacaaattat aaatatgtgg caggaagtag gaaaagcaat gtatgcccct   5400
cccatcagag gacaaattag atgctcatca aatattacag ggctaatatt aacaagagat   5460
ggtggaacta acagtagtgg gtccgagatc ttcagacctg ggggaggaga tatgagggac   5520
aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca   5580
cccaccaagg caaaaagaag agtggtgcag agagaaaaaa gagcagtggg aacgatagga   5640
gctatgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaataacg   5700
ctgacggtac aggccagact attattgtct ggtatagtgc aacagcagaa caatttgctg   5760
agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc   5820
caggcaagag tcctggctct ggaaagatac ctaagggatc aacagctcct agggatttgg   5880
ggttgctctg gaaaactcat ctgcaccact gctgtgcctt ggaatgctag ttggagtaat   5940
aaaactctgg atatgatttg ggataacatg acctggatgg agtgggaaag agaaatcgaa   6000
aattacacag gcttaatata caccttaatt gaagaatcgc agaaccaaca agaaaagaat   6060
gaacaagact tattagcatt agataagtgg gcaagtttgt ggaattggtt tgacatatca   6120
aattggctgt ggtgtataaa aatcttcata atgatagtag gaggcttgat aggtttaaga   6180
atagttttta ctgtactttc tatagtaaat agagttaggc agggatactc accattgtca   6240
tttcagaccc acctcccagc cccgagggga cccgacaggc ccgaaggaat cgaagaagaa   6300
ggtggagaca gagacagaga cagatccgtg cgattagtgg atggatcctt agcacttatc   6360
tgggacgatc tgcggagcct gtgcctcttc agctaccacc gcttgagaga cttactcttg   6420
attgtaacga ggattgtgga acttctggga cgcagggggt gggaagccct caaatattgg   6480
tggaatctcc tacagtattg gagtcaggag ctaaagaata gtgctgttag cttgctcaat   6540
gccacagcta tagcagtagc tgaggggaca gatagggtta tagaagtagt acaaggagct   6600
tatagagcta ttcgccacat acctagaaga ataagacagg gcttggaaag gattttgcta   6660
taagatgggt ggctagcccc gggtgataaa cggaccgcgc aatccctagg ctgtgccttc   6720
tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc   6780
cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg   6840
tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa   6900
tagcaggcat gctggggatg cggtgggctc tatataaaaa acgcccggcg gcaaccgagc   6960
gttctgaacg ctagagtcga caaattcaga agaactcgtc aagaaggcga tagaaggcga   7020
tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc   7080
cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtctgcca   7140
cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg   7200
gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga   7260
gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat   7320
cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt   7380
cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg   7440
atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca   7500
atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc   7560
ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg   7620
acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg   7680
catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag   7740
cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg   7800
tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcgag aaagccatcc   7860
agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt   7920
cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta   7980
cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca   8040
tccggggtca gcaccgtttc tgcggactgg ctttctacgt gaaaaggatc taggtgaaga   8100
tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt   8160
cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct   8220
gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc   8280
taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc   8340
ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc   8400
tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg   8460
ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt   8520
cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg   8580
agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg   8640
gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt   8700
atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag   8760
gggggcggag cctatggaaa acgccagcaa cgcggccttt ttacggttcc tgggcttttg   8820
ctggcctttt gctcacatgt tgtcgaccga caatattggc tattggccat tgcatacgtt   8880
gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg   8940
acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc   9000
atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctcgtg accgcccaac   9060
gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact   9120
ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa   9180
gtgtatcata tgccaagtcc gcccctattg acgtcaatga cggtaaatgg cccgcctggc   9240
attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag   9300
tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt   9360
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc   9420
accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg   9480
gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga   9540
tcgc                                                                9544
<210>  5
<211>  9918
<212>  DNA
<213>  Artificial
<220>
<223>  Construct of vaccine vector pGA1 and vaccine insert expressing cl
ade B HIV-1 Gag-Po
<220>
<221>  misc_feature
<222>  (106)..(1641)
<223>  encodes proteins of viral inner core
<220>
<221>  misc_feature
<222>  (1401)..(3617)
<223>  encodes viral proteins but not integrase
<220>
<221>  misc_feature
<222>  (3708)..(5715)
<223>  regulates high-level production of HIV genes
<220>
<221>  misc_featre
<222>  (3847)..(5944)
<223>  encodes protein regulating the transfer of RNA to cytoplasm
<220>
<221>  misc_feature
<222>  (3939)..(4181)
<223>  gene participates in viral assembly and budding
<220>
<221>  misc_feature
<222>  (4099)..(4941)
<223>  encodes truncated form of viral coat protein
<220>
<221>  misc_feature
<222>  (6098)..(9918)
<223>  vaccine vector pGA1
<400>  5
atcgatgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg     60
tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg    120
tcagtattaa gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga    180
aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca    240
gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa    300
ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc    360
tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag    420
gaagagcaaa acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc    480
agtcaggtca gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag    540
gccatatcac ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc    600
ccagaagtaa tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac    660
accatgctaa acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc    720
aatgaggaag ctgcagaatg ggatagagta catccagtgc atgcagggcc tattgcacca    780
ggccagatga gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa    840
caaataggat ggatgacaaa taatccacct atcccagtag gagaaattta taaaagatgg    900
ataatcctgg gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata    960
agacaaggac caaaagaacc ttttagagac tatgtagacc ggttctataa aactctaaga   1020
gccgagcaag cttcacagga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat   1080
gcgaacccag attgtaagac tattttaaaa gcattgggac cagcggctac actagaagaa   1140
atgatgacag catgtcaggg agtaggagga cccggccata aggcaagagt tttggctgaa   1200
gcaatgagcc aagtaacaaa tacagctacc ataatgatgc agagaggcaa ttttaggaac   1260
caaagaaaga tggttaagag cttcaatagc ggcaaagaag ggcacacagc cagaaattgc   1320
agggccccta ggaaaaaggg cagctggaaa agcggaaagg aaggacacca aatgaaagat   1380
tgtactgaga gacaggctaa ttttttaggg aagatctggc cttcctacaa gggaaggcca   1440
gggaattttc ttcagagcag accagagcca acagccccac catttcttca gagcagacca   1500
gagccaacag ccccaccaga agagagcttc aggtctgggg tagagacaac aactccccct   1560
cagaagcagg agccgataga caaggaactg tatcctttaa cttccctcag atcactcttt   1620
ggcaacgacc cctcgtcaca ataaagatag gggggcaact aaaggaagct ctattagata   1680
caggagcaga tgatacagta ttagaagaaa tgagtttgcc aggaagatgg aaaccaaaaa   1740
tgataggggg aattggaggt tttatcaaag taagacagta tgatcagata ctcatagaaa   1800
tctgtggaca taaagctata ggtacagtat tagtaggacc tacacctgtc aacataattg   1860
gaagaaatct gttgactcag attggttgca ctttaaattt tcccattagc cctattgaga   1920
ctgtaccagt aaaattaaag ccaggaatgg atggcccaaa agttaaacaa tggccattga   1980
cagaagaaaa aataaaagca ttagtagaaa tttgtacaga aatggaaaag gaagggaaaa   2040
tttcaaaaat tgggcctgag aatccataca atactccagt atttgccata aagaaaaaag   2100
acagtactaa atggagaaaa ttagtagatt tcagagaact taataagaga actcaagact   2160
tctgggaagt tcaattagga ataccacatc ccgcagggtt aaaaaagaaa aaatcagtaa   2220
cagtactgga tgtgggtgat gcatattttt cagttccctt agatgaagac ttcaggaagt   2280
atactgcatt taccatacct agtataaaca atgagacacc agggattaga tatcagtaca   2340
atgtgcttcc acagggatgg aaaggatcac cagcaatatt ccaaagtagc atgacaaaaa   2400
tcttagagcc ttttaaaaaa caaaatccag acatagttat ctatcaatac atgaacgatt   2460
tgtatgtagg atctgactta gaaatagggc agcatagaac aaaaatagag gagctgagac   2520
aacatctgtt gaggtgggga cttaccacac cagacaaaaa acatcagaaa gaacctccat   2580
tcctttggat gggttatgaa ctccatcctg ataaatggac agtacagcct atagtgctgc   2640
cagaaaaaga cagctggact gtcaatgaca tacagaagtt agtggggaaa ttgaataccg   2700
caagtcagat ttacccaggg attaaagtaa ggcaattatg taaactcctt agaggaacca   2760
aagcactaac agaagtaata ccactaacag aagaagcaga gctagaactg gcagaaaaca   2820
gagagattct aaaagaacca gtacatggag tgtattatga cccatcaaaa gacttaatag   2880
cagaaataca gaagcagggg caaggccaat ggacatatca aatttatcaa gagccattta   2940
aaaatctgaa aacaggaaaa tatgcaagaa tgaggggtgc ccacactaat gatgtaaaac   3000
aattaacaga ggcagtgcaa aaaataacca cagaaagcat agtaatatgg ggaaagactc   3060
ctaaatttaa actacccata caaaaggaaa catgggaaac atggtggaca gagtattggc   3120
aagccacctg gattcctgag tgggagtttg ttaatacccc tcctttagtg aaattatggt   3180
accagttaga gaaagaaccc atagtaggag cagaaacctt ctatgtagat ggggcagcta   3240
acagggagac taaattagga aaagcaggat atgttactaa caaaggaaga caaaaggttg   3300
tccccctaac taacacaaca aatcagaaaa ctcagttaca agcaatttat ctagctttgc   3360
aggattcagg attagaagta aacatagtaa cagactcaca atatgcatta ggaatcattc   3420
aagcacaacc agataaaagt gaatcagagt tagtcaatca aataatagag cagttaataa   3480
aaaaggaaaa ggtctatctg gcatgggtac cagcacacaa aggaattgga ggaaatgaac   3540
aagtagataa attagtcagt gctggaatca ggaaaatact atttttagat ggaatagata   3600
aggcccaaga tgaacattag aattctgcaa caactgctgt ttatccattt tcagaattgg   3660
gtgtcgacat agcagaatag gcgttactcg acagaggaga gcaagaaatg gagccagtag   3720
atcctagact agagccctgg aagcatccag gaagtcagcc taaaactgct tgtaccaatt   3780
gctattgtaa aaagtgttgc tttcattgcc aagtttgttt cataacaaaa gccttaggca   3840
tctcctatgg caggaagaag cggagacagc gacgaagacc tcctcaaggc agtcagactc   3900
atcaagtttc tctatcaaag cagtaagtag tacatgtaat gcaacctata caaatagcaa   3960
tagtagcatt agtagtagca ataataatag caatagttgt gtggtccata gtaatcatag   4020
aatataggaa aatattaaga caaagaaaaa tagacaggtt aattgataga ctaatagaaa   4080
gagcagaaga cagtggcaat gagagtgaag gagaaatatc agcacttgtg gagatggggg   4140
tggagatggg gcaccatgct ccttgggatg ttgatgatct gtagtgctac agaaaaattg   4200
tgggtcacag tctattatgg ggtacctgtg tggaaggaag caaccaccac tctattttgt   4260
gcatcagatg ctaaagcata tgatacagag gtacataatg tttgggccac acatgcctgt   4320
gtacccacag accccaaccc acaagaagta gtattggtaa atgtgacaga aaattttaac   4380
atgtggaaaa atgacatggt agaacagatg catgaggata taatcagttt atgggatcaa   4440
agcctaaagc catgtgtaaa attaacccca ctctgtgtta gtttaaagtg cactgatttg   4500
aagaatgata ctaataccaa tagtagtagc gggagaatga taatggagaa aggagagata   4560
aaaaactgct ctttcaatat cagcacaagc ataagaggta aggtgcagaa agaatatgca   4620
tttttttata aacttgatat aataccaata gataatgata ctaccagcta tacgttgaca   4680
agttgtaaca cctcagtcat tacacaggcc tgtccaaagg tatcctttga gccaattccc   4740
atacattatt gtgccccggc tggttttgcg attctaaaat gtaataataa gacgttcaat   4800
ggaacaggac catgtacaaa tgtcagcaca gtacaatgta cacatggaat taggccagta   4860
gtatcaactc aactgctgtt aaatggcagt ctggcagaag aagaggtagt aattagatct   4920
tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag   4980
tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga   5040
gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa   5100
gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta   5160
tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac   5220
tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa   5280
aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg   5340
tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct   5400
ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag   5460
aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa   5520
gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga   5580
tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctgta gtgaatagag   5640
ttaggcaggg atattcacca ttatcgtttc agacccacct cccaatcccg aggggacccg   5700
acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat   5760
tagtgaacgg atccttagca cttatctggg acgatctgcg gagcctgtgc ctcttcagct   5820
accaccgctt gagagactta ctcttgattg taacgaggat tgtggaactt ctgggacgca   5880
gggggtggga agccctcaaa tattggtgga atctcctaca gtattggagt caggagctaa   5940
agaatagtgc tgttagcttg ctcaatgcca cagctatagc agtagctgag gggacagata   6000
gggttataga agtagtacaa ggagcttata gagctattcg ccacatacct agaagaataa   6060
gacagggctt ggaaaggatt ttgctataag atgggtggct agccccgggt gataaacgga   6120
ccgcgcaatc cctaggctgt gccttctagt tgccagccaa actgttgttt gcccctcccc   6180
cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga   6240
aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga   6300
cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat   6360
ataaaaaacg cccggcggca accgagcgtt ctgaacgcta gagtcgacaa attcagaaga   6420
actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa   6480
gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca   6540
acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa   6600
agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat   6660
cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct   6720
gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc   6780
gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca   6840
gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca   6900
ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa   6960
cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct   7020
cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc   7080
cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt   7140
catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt   7200
caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc   7260
agatccttgg cggcgagaaa gccatccagt ttactttgca gggcttccca accttaccag   7320
agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct   7380
atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg   7440
tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt   7500
tctacgtgaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt   7560
aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt   7620
gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag   7680
cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca   7740
gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca   7800
agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg   7860
ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg   7920
cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct   7980
acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga   8040
gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc   8100
ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg   8160
agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg   8220
cggccttttt acggttcctg ggcttttgct ggccttttgc tcacatgttg tcgaccgaca   8280
atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg   8340
gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat   8400
caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg   8460
taaatggccc gcctcgtgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta   8520
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg   8580
gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc ccctattgac   8640
gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt   8700
cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg   8760
cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc   8820
attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt   8880
aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata   8940
agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac   9000
ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg   9060
cggattcccc gtgccaagag tgacgtaagt accgcctata gactctatag gcacacccct   9120
ttggctctta tgcatgctat actgtttttg gcttggggcc tatacacccc cgctccttat   9180
gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt attgaccact   9240
cccctattgg tgacgatact ttccattact aatccataac atggctcttt gccacaacta   9300
tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac tctgtatttt   9360
tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg ccgtcccccg   9420
tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg gtacgtgttc   9480
cggacatggg ctcttctccg gtagcggcgg agcttccaca tccgagccct ggtcccatgc   9540
ctccagcggc tcatggtcgc tcggcagctc cttgctccta acagtggagg ccagacttag   9600
gcacagcaca atgcccacca ccaccagtgt gccgcacaag gccgtggcgg tagggtatgt   9660
gtctgaaaat gagctcggag attgggctcg caccgtgacg cagatggaag acttaaggca   9720
gcggcagaag aagatgcagg cagctgagtt gttgtattct gataagagtc agaggtaact   9780
cccgttgcgg tgctgttaac ggtggagggc agtgtagtct gagcagtact cgttgctgcc   9840
gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc catgggtctt   9900
ttctgcagtc accgtcca                                                 9918
<210>  6
<211>  35
<212>  DNA
<213>  Artificial
<220>
<223>  introduced lambda T0 terminator sequence
<400>  6
ataaaaaacg cccggcggca accgagcgtt ctgaa                                35
<210>  7
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer for site-directed mutagenesis for introducing Cla I site
<400>  7
ccgtcagatc gcatcgatac gccatccacg                                      30
<210>  8
<211>  30
<212>  DNA
<213>  Artificial
<220>
<223>  primer for site-directed mutagenesis to introduce Cla I site
<400>  8
cgtggatggc gtatcgatgc gatctgacgg                                      30
<210>  9
<211>  29
<212>  DNA
<213>  Artificial
<220>
<223>  Sense primer
<400>  9
gagctctatc gatgcaggac tcggcttgc                                       29
<210>  10
<211>  31
<212>  DNA
<213>  Artificial
<220>
<223>  antisense primer
<400>  10
ggcaggtttt aatcgctagc ctatgctctc c                                    31
<210>  11
<211>  17
<212>  DNA
<213>  Artificial
<220>
<223>  sense primer
<400>  11
gggcaggagt gctagcc                                                    17
<210>  12
<211>  29
<212>  DNA
<213>  Artificial
<220>
<223>  antisense primer
<400>  12
ccacactact ttcggaccgc tagccaccc                                       29
<210>  13
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  C15S ZN1 primer
<400>  13
ggttaagagc ttcaatagcg gcaaagaagg gc                                   32
<210>  14
<211>  32
<212>  DNA
<213>  Artificial
<220>
<223>  C15S ZN2 primer
<400>  14
gcccttcttt gccgctattg aagctcttaa cc                                   32
<210>  15
<211>  27
<212>  DNA
<213>  Artificial
<220>
<223>  C36S ZN3 primer
<400>  15
gggcagctgg aaaagcggaa aggaagg                                         27
<210>  16
<211>  27
<212>  DNA
<213>  Artificial
<220>
<223>  C36S ZN4 primer
<400>  16
ccttcctttc cgcttttcca gctgccc                                         27
<210>  17
<211>  44
<212>  DNA
<213>  Artificial
<220>
<223>  D185N RT1 primer
<400>  17
ccagacatag ttatctatca atacatgaac gatttgtatg tagg                      44
<210>  18
<211>  44
<212>  DNA
<213>  Artificial
<220>
<223>  D185N RT2 primer
<400>  18
cctacataca aatcgttcat gtattgatag ataactatgt ctgg                      44
<210>  19
<211>  33
<212>  DNA
<213>  Artificial
<220>
<223>  W266T RT3 primer
<400>  19
ggggaaattg aataccgcaa gtcagattta ccc                                  33
<210>  20
<211>  33
<212>  DNA
<213>  Artificial
<220>
<223>  W266T RT4 primer
<400>  20
gggtaaatct gacttgcggt attcaatttc ccc                                  33
<210>  21
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  E478Q RT5 primer
<400>  21
ccctaactaa cacaacaaat cagaaaactc agttacaagc                           40
<210>  22
<211>  40
<212>  DNA
<213>  Artificial
<220>
<223>  E478Q RT6 primer
<400>  22
gcttgtaact gagttttctg atttgttgtg ttagttaggg                           40
<210>  23
<211>  34
<212>  DNA
<213>  artificial
<220>
<223>  D25A Prt1 primer
<400>  23
ggcaactaaa ggaagctcta ttagccacag gagc                                 34
<210>  24
<211>  34
<212>  DNA
<213>  Artificial
<220>
<223>  D25 Aprt2 primer
<400>  24
gctcctgtgg ctaatagagc ttcctttagt tgcc                                 34