Appendix 3-10 
Quarantine Requirements for the Im portation of Frozen Deer Semen 
(In case of any discrepancy between the English version and the Chinese text of these Requirem ents, the Chinese text shall gove rn.) 
Promulgated by Council of  Agriculture on May 21, 2008 
 
1. Before the importation of frozen deer se men, it is required to apply to the animal 
quarantine authority of this country fo r issuance of quarantine requirements and 
the importation of the frozen semen sh all be in compliance with this 
requirements. 
 
2. The frozen deer semen is prohibited fr om importation from the countries or zones 
where have been recognized by central comp etent authority of this country as a 
country (zone) infected with foot and mouth disease, rinderpest, contagious 
bovine pleuropneumonia. 
 
3. The donor deer shall be in compliance with following requirements: 
(1) The donor deer shall be originated fro m the countries or zones where chronic 
wasting disease of deer has not known to  occur for more than five years. 
(2) The donor deer shall be originated fro m the states or province where vesicular 
stomatitis has not occurred in the previous year. 
(3) The donor deer shall have been raised in  the artificial insemination centers for at 
least one year and shall have no t been used for natural mating. 
(4) The donor deer shall have been raised for one year prior to semen collection or 
since birth in the artificial inse mination centers, which are under the 
supervision and regular inspection ¡]including microbiological and 
parasitological tests and necropsies ¡^ by the competent authority of the 
exporting country. The artificial insemina tion center shall be complied with the 
following requirements: 
A. Louping ill, bluetongue, bovi ne tuberculosis, parat uberculosis (Johne's 
disease), malignant catarrhal fever, Bo rna disease, brucellosis and Q fever 
have not occurred in the previous year. 
B. Cervine herpesvirus infection, infec tious bovine rhinotracheitis, bovine viral 
diarrhea, peste des petits ruminants, epizoo tic hemorrhagic disease of deer, bovine ephemeral fever, bovine genita l campylobacteriosis, leptospirosis 
and trichomonosis have not occu rred during the past 6 months. 
(5) The donor deer and non-donor deer whic h have been raised in same artificial 
insemination center shall not be vaccinat ed against foot and mouth disease and 
with any other vaccine that has not been approved by animal quarantine 
authority of this country. 
(6) The donor deer shall be inspected a nd found free from clinical evidence of any 
communicable disease and subject to th e following tests and treatment within 
thirty days prior to semen collection: 
A. The donor deer shall be subjected to the following tests with negative 
results: 
a) Foot and mouth disease: serum neutralization test. 
b) Vesicular stomatitis: se rum neutralization test. 
c) Bluetongue: ELISA. 
d) Paratuberculosis (J ohne's disease): ELISA. 
e) Bovine brucellosis: ELISA or complement fixation test. 
f) Bovine tuberculosis: intr adermal tuberculin test. 
g) Cervine herpesvirus infection: for each individual donor a pooled sample 
of at least ten percent  of the semen from all the ej aculates used in this 
consignment was tested (with a minimu m of five straws) for the presence 
of cervine herpes virus, by tissue culture inoculation (in BEL cells 
demonstrated to support growth of cervi ne herpes virus) with at least one 
blind passage, with  negative results.  
h) Epizootic hemorrhagic dis ease of deer: ELISA or AGID. 
i) Louping ill: hemagglutination inhibition test. j) Other diseases and diagnostic test s provisionally designated by animal 
quarantine authority of this country according to the world animal disease 
epidemiology. 
B. The donor deer should be injected with long acting oxytetracycline (20 
mg/kg) not less 14 days and not mo re than 30 days prior to semen 
collection. 
(7) The donor deer shall have been subject ed to veterinary examination before 
semen collection, and show no clinical evidence or symptom of any infectious, contagious or venereal diseases. 
 
4. The deer semen shall be  collected, handled, preser ved and transported under a 
sanitary condition free from contamin ation by pathogenic organisms. 
 
5. The deer semen diluents shall not be contaminated by any pathogen of Newcastle 
disease, highly pathogenic avian influe nza, foot and mouth disease, bovine 
spongiform encephalopathy and chronic wasting disease of deer. 
 
6. To import the deer semen, an original veterinary certificate issued by the animal 
quarantine authority of the exporting count ry shall be provided and the certificate 
shall state the following detaile d information in English: 
(1) Origin: 
A. Species of the donor deer and identification number. 
B. Date of semen collection, amount an d identification number of the exported 
ampoules, vials or straws. 
C. Exporting country. 
D. Competent authority of the exporting country. 
E. Name and address of the artificial in semination center that the donor deer was 
raised. 
F. Name and address of the artificial insemination center. 
G. Name and address of the semen storage premises. H. Name and address of the expor ter or the exporting company. 
(2) Destination: 
A. Country of destination. B. Name and address of th e importer or the company. 
(3) Results of quarantine inspection: 
A. Dates of semen collection, name of th e test laboratory, and methods, dates, 
and results of the test. 
B. Attesting that the health status of the donor deer and seme n are in compliance 
with Article 3 to Article 5 of the requirements. 
(4) Kinds of vaccines administer ed and date of vaccination. 
(5) Date, place and authority of issuance of the veterinary certificate, official stamp, and the name and signature of the veterinary officer. 
 
7. The storage and shipment of frozen deer  semen shall be in compliance with the 
followings: 
(1) The registered or official veterina rians of exporting country shall seal the 
ampoules, vials or straws containing th e semen immediately after the semen 
collection. 
(2) The following information shall be indelibly marked on the sealed ampoules, 
vials or straws containing the semen: breed, identification number of donor 
deer, collection date, and name of the artificial insemination center that the 
donor deer was raised. 
(3) The sealed ampoules, vials or straws containing the semen shall be securely 
packed in the container, which has been  cleaned and sterilized  before use, and 
only new liquid nitrogen may be adde d to the container. The following 
information shall be marked on th e container: the name, breed, and 
identification number of donor deer, collec tion date, and name of the artificial 
insemination center that the donor deer raised on, the amount of the sealed 
ampoules, vials or straws, the country of destination, the na me and address of 
the importer. 
(4) The sealed semen shall be stored in the place approved by the exporting country 
before shipment. 
 
8. The management of artificial insemination centers and the procedures of collection, 
handling, and preservation of deer se men shall be in compliance with the 
conditions designated in A ppendix 3.2.1 of the OIE Terrestrial Animal Health 
Code or with the regulations recognized by this country as equivalent to the above 
conditions. 
 9. Regarding the diseases te st, quarantine measures and tr eatments stated in Article 3 
of this Requirements, the competent aut hority of the exporti ng country may apply 
to the animal quarantine authority of this country for exemption of specific 
diseases test due to the disease free status  or to test with an alternative method. 
 For the said application, the competent authority of the exporting country shall 
submit to the animal quarantine authority of this country with documents that 
testify its free status which complies with the terrestrial code of OIE. The animal 
quarantine authority of this country may follow the terrestrial code of OIE to 
review the said application. The animal quarantine authority of this country may 
send inspectors to the exporting country to  conduct on-site inspection if necessary. 
The expense shall be borne by the exporting country.