1  Calcium L-Tartrate 
Ｌ-酒石酸カルシウム 
 
 
Standards for Use  
Permitted for use only in grape wine. Must be used at not more than 2.0 g/L in grape wine as 
calcium L-tartrate. 
 
 
Compositional Specifications 
Substance Name  Calcium L-Tartrate 
Structural Formula 
 
OOCCOOOH H
HOHCa2•nH2O
n=2or 4 
 
Molecular Formula  C4H4CaO6•nH2O (n= 2 or 4) 
Molecular Weight  Dihydrate 224.18  
Tetrahydrate 260.21 
Chemical Name [CAS number] 
Calcium(2R, 3R)-2,3-dihydroxybutanedioate dihydrate 
Calcium(2R, 3R)-2,3-dihydroxybutanedioate tetrahydrate [5892-21-7] 
Content  Calcium L-Tartrate, when dried, contains not less than 98.0% of calcium L-tartrate 
(C4H4CaO6). 
Description  Calcium L-Tartrate occurs as a white to grayish white powder. 
Identification 
(1) Dissolve 1 g of Calcium L-Tartrate by adding hydrochloric acid TS (1 mol/L) to make 50 
mL. This solution is dextrorotatory. 
(2) Calcium L-Tartrate responds to test (1) for Calcium Salt in the Qualitative Tests. 
(3) A solution of 1 g of Calcium L-Tartrate in 50 mL of hydrochloric acid TS (1 mol/L) 
responds to test (3) for Tartrate in the Qualitative Tests. 
Specific Rotation  [α]: +6.2 to +7.4. Attachment 2 
 
  Weigh accurately about 1 g of Calcium L-Tartrate, and dissolve it by adding hydrochloric 
acid TS (1 mol/L) to make exactly 50 mL. Measure the optical rotation of this solution. 
pH  6.0–9.5. 
Test Solution  To 3.0 g of Calcium L-Tartrate, add 60 mL of water, shake it for 1 hour, and 
centrifuge for 5 minutes at 3000 rpm. Use the supernatant. 
Purity  
(1) Lead  Not more than 5 μg/g as Pb (0.80 g, Method 3, Control Solution: Lead 
Standard Solution 4.0 mL, Flame Method). 
(2) Arsenic  Not more than 3 μg/g as As (0.50 g, Standard Color: Arsenic Standard Solution 
3.0 mL, Apparatus B). 
Test Solution  Dissolve the specified amount of Calcium L-Tartrate by adding 5 mL of 
diluted hydrochloric acid (1 in 4).   
(3) Sulfate  Not more than 0.1% as SO4. 
Test Solution  Dissolve 1.2 g of Calcium L-Tartrate by adding 30 mL of hydrochloric acid 
TS (1 mol/L), and further add hydrochloric acid TS (1 mol/L) to make 50 mL. 
Control Solution  To 2.5 mL of 0.005 mol/L sulfuric acid, add hydrochloric acid TS (1 mol/L) 
to make 50 mL.  
(4) Basic residues  Not more than 3% as CaCO3. 
Weigh accurately about 2 g of Calcium L-Tartrate in a container, add gradually exactly 
measured 25 mL of 1 mol/L hydrochloric acid, and heat the container with the solution in a 
water bath for about 10 minutes. After cooling, titrate the excess hydrochloric acid with 1 mol/L 
sodium hydroxide (indicator: 4–5 drops of methyl red TS). The endpoint is when the color of 
the solution changes from red to yellow. Separately, perform a blank test, and determine the 
amount of basic residues by the formula: 
The amount of basic residues (CaCO3) =(  ) × .
 ()     
a : volume (mL) of 1 mol/L sodium hydroxide consumed in the blank test, 
b : volume (mL) of 1 mol/L sodium hydroxide consumed in the test. 
 
Loss on Drying  Not more than 30.0% (200C, 7 hours). 
Assay   
Test Solution  Weigh accurately about 1 g of Calcium L-Tartrate, mix it with 8 mL of diluted 
hydrochloric acid (1 in 4), and dissolve by adding about 20 mL of water. Warm if necessary to 
dissolve it, and cool to room temperature. To this solution, further add water to make exactly 
50 mL. 
Procedure  Quantify calcium L-tartrate in the sample by Method 1 of Calcium Salt 
Determination. Calculate the content on dried basis. 
Each mL of 0.05 mol/L of disodium dihydrogen ethylenediaminetetraacetate = 9.407 mg of 
C4H4CaO6