26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  
Certificate: Health certificate 1 - Commercial chicken  
This health certificate relates to the pre -collection and collection preparation of the source flock of 
eggs for this consignment and must accompany the consignment to Australia. The health certificate 
must be completed and signed by the owner/manager, the Government Approved Veterinarian 
supervising the facility and the Official Veterinarian (Government Veterinary Officer employed b y the 
government veterinary authority) and must accompany the consignment of eggs to Australia.  
Where options exist, the health certificate must indicate the option that applies.  
 
I. Identification of the consignment  
Import permit number: ………………………  
Number of eggs  Mark(s)  Collection dates(s)  
      
      
      
II. Origin of the eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Address of post-arrival quarantine isolation facility:  
Nature and identification of transport:  
Number and type of containers:  
Identification of container seals:  
IV. Health Certification  
Part A. Declaration by the owner or manager of the source flock.  
I, ............. ................................................. ( name ), the owner/manager ( delete one ) of the source flock 
from which the eggs to be exported to Australia were derived, hereby declare that:  
1. The source flock has not been vaccinated against avian influe nza. 
 
2. The source flock:  
 has not been vaccinated against Newcastle disease (APMV -1) 
 
or 
 has been vaccinated against Newcastle disease (APMV -1)  
  
Page  2 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  [The health certificate must indicate the option that applies].  
 
3. The source flock:  
 has not been vaccinated against avian pneumovirus  
 
or 
 has been vaccinated against avian pneumovirus.  
[The health certificate must indicate the option that applies].  
 
4. The vaccination history of the source flock for Newcastle disease (APMV -1) and avian pn eumovirus 
is as follows OR is attached to this health certificate. Vaccination schedule(s) of other diseases are 
attached.  
[The health certificate must indicate the option that applies].  
Disease  Date(s) of vaccination  Name and type of vaccine  
 Newcastle d isease (APMV -1)     
 Avian pneumovirus      
 
5. The eggs have been laid by a source flock established and maintained as an all -in-all-out flock, the 
youngest birds being not less than 32 weeks old when eggs were collected.  
 
Signature: ........................................................……… Date: ..................................  
Owner/Manager  
 
Name: ......................................................................….…………………………..........  
Address: ................................ .....................................…………………………….......  
......................................................................………………………….............................  
 
Part B: Flock status and disease testing. Certification by the Government Approved 
Veteri narian or Official Veterinarian supervising the source flock facility.  
I, ............................................................ ( name ), a Government Approved Veterinarian or Official 
Veterinarian* of ................................................. ............ ( country of export ) hereby certify in relation to 
the consignment of hatching eggs of poultry identified on this health certificate that:  
 
[*the health certificate must indicate which option applies].  
1. The source flock, from which the eggs for export to Australia were derived, has been under my 
direct supervision for the 90 days immediately preceding the collection of eggs for this consignment 
and during collection, and, after due enquiry, I have no rea son to doubt the truth of the 
owner's/manager's declaration above.  
2. The source flock is housed in secure rodent -proof and bird -proof buildings within the primary 
breeding establishment. The primary breeding establishment is isolated by 400 metres from al l other 
neighbouring poultry farms and/or poultry breeding establishments unless these are shown by testing 
to be of a health status equal to the source flock.   
  
Page  3 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  3. All water supplied to the source flock is secure against contamination by wild birds. All bu ildings 
containing feed and feeding equipment for the source flock are also bird -proofed.  
4. A biosecurity program has been in place during the entire period under my direct supervision to 
minimise the introduction of disease. This included the use of dedi cated staff for the source flock, 
movement control and disinfection of vehicles not dedicated to the source flock facility, such as those 
for waste removal, feed delivery and service personnel vehicles.  
 
5. After due enquiry I am satisfied that there has been no epidemiological contact between the source 
flock and any premises on which clinical Newcastle disease or avian influenza has occurred from 90 
days prior to the commencement of egg collection until despatch of this consignment.  
 
6. The source flock has been free from clinical signs of the following diseases for the 90 day period 
prior to collection of the eggs and during egg collection for export to Australia and has not come into 
contact with any birds showing evidence of these diseases.  
 Avian influ enza  
 Newcastle disease (APMV -1) 
 Avian paramyxovirus type 3 (APMV -3)  
 Fowl typhoid ( Salmonella Gallinarum)  
 Pullorum disease ( Salmonella Pullorum)  
 Salmonella Enteritidis infection  
 Arizona disease ( Salmonella Arizonae)  
 Avian pneumovirus  
 Infectious bursal  disease  
 Mycoplasma  iowae  infection  
 Runting/stunting syndrome  
7. Avian influenza testing  
Not more than 21 days before the first day of collection of eggs for export to Australia, a sample of the 
source flock was tested serologically for influenza virus type A with negative results.  
Testing for influenza virus type A was conducted on a flock sample of a sufficient size to give a 99% 
confidence of detecting the disease if there was a 5% disease prevalence in the source flock.  
 
8. Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3) serology  
Vaccinated flocks:  
 Not more than 21 days prior to the first day of collection of eggs for export to Australia, a 
random sample of 100 individually identified birds in the source flock was tested serologically  
for Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3). Serological titres for 
each bird are attached to the health certificate and are consistent with vaccination against 
Newcastle disease virus (APMV -1). The laboratory report specifies the un its used for the titres 
and at what value they are considered positive.  
After sampling, the 100 individually identified birds were replaced randomly throughout the 
source flock. Testing is scheduled to be repeated on the same individually identified birds not 
less than 14 days after the collection of the last egg for this consignment, in accordance with 
Health Certificate 2.   
  
Page  4 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   
or 
 At least 14 days prior to the commencement of egg collection for this consignment, 
unvaccinated sentinel birds were placed evenly throughout the source flock at a rate of 1 
sentinel bird per 100 source flock birds with a minimum of 30 sentinel birds. Daily observation 
of these birds was undertaken with no evidence of clinical signs of Newcastle disease virus 
(APMV -1) or paramyxovirus  3 infections. On the first day of collection of eggs for this 
consignment, each sentinel bird was tested serologically for Newcastle disease virus (APMV -
1) and paramyxovirus 3 (APMV -3) with negative results. Testing is scheduled to be repeated 
on the same  sentinel birds not less than 14 days after the collection of the last egg for this 
consignment, in accordance with Health Certificate 2.  
Unvaccinated flocks:  
 Not more than 21 days before the first day of collection of eggs for export to Australia, a 
samp le of the source flock was tested serologically for Newcastle disease virus (APMV -1) and 
paramyxovirus 3 (APMV -3) with negative results. Testing was conducted on a flock sample of 
a sufficient size to give a  99% confidence of detecting the disease if there  was a 5% disease 
prevalence in the source flock.  
[The health certificate must indicate the option that applies].  
9. Salmonella  serology  
 The source flock is housed in an establishment that is subject to regular salmonella 
surveillance by serological testing of birds and/or environmental sampling and salmonella 
culture under a government scheme recognised by the Department of Agriculture, Wa ter and 
the Environment (Appendix 1). No serological or microbiological evidence of S. Pullorum, S. 
Gallinarum, or S. Enteritidis was detected in the birds or environment.  
 
Name of scheme/s as listed in the government scheme recognised by the Department of 
Agriculture, Water and the Environment (Appendix 1): 
 
……………………........................................................................................................  
 
Accreditation number/s: … …………………..................................................  
 
Last two sample dates: …………………….........../.......................................  
 
or 
 Not more than 21 days before the first day of collection of eggs for export to Australia, a 
sample of the sour ce flock was tested serologically for S. Pullorum, S. Gallinarum and  S. 
Enteritidis with negative results.  
The flock sample tested was of a sufficient size to give a 99% confidence of detecting the 
disease if there was a 0.5% disease prevalence in the sou rce flock. All birds that gave a 
positive or suspicious serological result for S. Pullorum, S. Gallinarum or S. Enteriditis were 
killed and their organs cultured and identified for these Salmonella  species. The results of the 
cultures and identifications a re attached and none of these species were identified.  
[The health certificate must indicate the option that applies].  
 
10. Salmonella Arizonae   
  
Page  5 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   The source flock is housed in an establishment that is subject to regular environmental 
sampling and salmonella culture under a government scheme recognised by the Department 
of Agriculture, Water and the Environment (Appendix 1) as being equivalent to the testing 
requirements. Salmonella Arizonae  species were not detected in the floc k or the establishment 
during the previous two samplings.  
 
Name of scheme/s as listed in the government scheme recognised by the Department of 
Agriculture, Water and the Environment (Appendix 1): 
 
……………………........................................................................................................  
 
Accreditation number/s: ……………………..................................................  
 
Last two sample dates: …………………….........../............... ........................  
 
or 
 Not more than 21 days before the first day of collection of eggs for export to Australia the 
source flock was determined to be free of infection with Salmonella Arizonae by culture and 
isolation from shed litter.  
The total numb er of samples cultured was: ……………………..  
20 samples, representative of the entire source flock, were collected. Each sample was a 
composite sample of 3 floor and 2 nest litter samples; i.e. a total of 60 floor locations and 40 
nest box locations.  
[The healt h certificate must indicate the option that applies].  
11. Avian pneumovirus  
 
For flocks NOT vaccinated against avian pneumovirus:  
Not more than 21 days prior to the first day of collection of eggs for export to Australia, the source 
flock was tested using  an ELISA test for avian pneumovirus.  
The sample tested was of a sufficient size to give a 99% confidence of detecting the disease if there 
was a 0.5% disease prevalence in the source flock.  
 
Number of samples: ...............  
Either:  
 All results were negative  
 
or 
 Any birds returning positive results were re -tested using the IFA test with negative results. 
Test results must be attached to the health certificate. A short explanation must be provided 
by the certifying vet as to why these re -tests confirm the positive results as false: 
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
………………………………………………………………….  
For flocks vaccinated against avian pneumovirus:  
No more than 21 days prio r to the first day of collection of eggs for export to Australia, a random 
sample of 100 individually identified birds in the source flock were serologically tested for avian 
pneumovirus using the ELISA method.   
  
Page  6 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Test results for each bird are attached to t he health certificate and are consistent with vaccination 
against avian pneumovirus. The laboratory report specifies the units used for the titres and at what 
value they are considered positive. After sampling, the 100 individually identified birds were re placed 
randomly throughout the source flock. Testing is scheduled to be repeated on the same individually 
identified birds not less than 14 days after collection of the last egg for this consignment, in 
accordance with Health Certificate 2.  
 
[The health ce rtificate must indicate the option that applies].  
 
12. All tests were:  
 carried out in a government laboratory or a laboratory approved by the government of the 
exporting country for this specific purpose, and agreed to by the Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 2), or tests agreed to by the Department of Agriculture, Water 
and the Environment.  
Test results are shown in the table below. Laboratory reports must also be attached to the health 
certificate and forwarded to the Department of Agriculture, Water and the Environment.  
Total number of birds in the source flock: ………………………………….  
Disease  Laboratory  Test 
used  No of 
tests  No of positives 
results  Details of any re -
testing  
Avian Influenza  
(Influenza virus type A)  
           
 
Newcastle disease virus  
(APMV -1) 
           
 
APMV -3 
           
 
Salmonella Pullorum  
           
 
Salmonella Gallinarum  
           
 
Salmonella Enteritidis  
           
 
Salmonella Arizonae  
           
 
Avian pneumovirus  
           
  
  
Page  7 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  13. The eggs for export to Australia were collected over a period of no more than 14 days. The eggs 
for export to Australia were collected separately to floor and dirty eggs. No floor or dirty eggs are 
included in this consignment of eggs for export to Austra lia. 
14. The eggs in this consignment were clean and were not washed or cleaned after collection.  
15. The eggs were collected, indelibly marked and dispatched under the supervision of a Government 
Veterinary Officer of the country of export.  
16. After coll ection, the eggs in this consignment were stacked on new egg flats (or egg flats that 
were cleaned and disinfected according to the requirements in this clause, prior to use) to permit air 
circulation and, within eight hours of lay, were either:  
 fumigated with formaldehyde gas in accordance with the OIE recommendations for the 
sanitation of hatching eggs and hatchery equipment.  
 
or 
 disinfected using Virkon  
 
or 
 disinfected using an agent approved by the Department of Agriculture, Water and the 
Environment. Written approval must be attached to the health certificate.  
Agent: ..........................................................................................................  
[The health certificate must indicate the option that applies].  
17. After fumigat ion/ disinfection, eggs were cooled to storage temperature and then packaged in the 
room they were fumigated/ disinfected into new crates with new, unused separators and sealed in 
leak-proof egg boxes for transport to Australia.  
 The eggs were handled and p acked in a manner to avoid any contamination.  
 The sealed boxes were secured in isolation from other birds and eggs until dispatch.  
 To prevent any leakage if damage to the eggs occurs during transport either:  
- the sealed egg boxes were placed in plastic bags, sealed and packed into approved solid -
sided aircraft containers  
or 
- the sealed egg boxes were packed into approved solid -sided aircraft containers lined with 
plastic to prevent any leakage if damage to the eggs occurs during transport.  
Signature: .. .....................................................…….. Date: ........................................  
Government Approved Veterinarian or Official Veterinarian  
 
Name: ……………………………........................................................................... ...........  
Address: ………………………….....................................................................................  
……………………………………..........................................................................................  
Email and contact number: …………………… ………………..............................  
............................................................................................................................. ................  
[NOTE: All pages  are to be endorsed with the Official Stamp].  
Part C: Disease  status of the country of origin. Certification by the Official Veterinarian.   
  
Page  8 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  I, ............................................................ ( name ), an Official Veterinarian of 
.............................................................( certifying minis try or department ) hereby certify in relation to 
the consignment of hatching eggs of poultry identified on this health certificate that:  
1. Avian influenza  
Either  
 The country of export is officially free of notifiable highly pathogenic avian influenza type  A 
infection in commercial poultry, game birds and pet birds.  
 
and 
 No case of clinical avian influenza has occurred in poultry at the premises of origin or within a 
radius of 40 km thereof during the period commencing 21 days prior to the start of collect ion of 
eggs for this consignment until today.  
 
or 
 The flock(s) of origin are in a zone or compartment approved by the Department of Agriculture, 
Water and the Environment.  
[The health certificate must indicate the option that applies].  
2. Newcastle disease  
No case of clinical Newcastle disease (APMV -1) has occurred in poultry at the premises of origin or 
within a radius of 40 km thereof during the period commencing 21 days prior to the start of collection 
of eggs for this consignment until today.  
Signature : .......................................................…….. Date: ........................................  
Official Veterinarian  
 
Name: ……………………………......................................................................................  
Address: …………………………. ....................................................................................  
……………………………………..........................................................................................  
Email and contact number: ……………………………………..............................  
............................................................................................................................. ................  
Part D: Sealing approved aircraft containers at the airport and trave l to Australia. Certification 
by the Government Approved Veterinarian or Official Veterinarian.  
I, the undersigned Official Veterinarian, certify that with respect to the hatching eggs of poultry 
described in this health certificate number ……………………………………… ……………, the 
individually sealed boxes containing the eggs were loaded onto solid -sided airline approved aircraft 
container(s) which were sealed with official government seals.  
Identification numbers of official government seals on aircraft containers (numbe rs) are: 
........................................................………  
I have checked the flight schedule/airway bill and can confirm that:  
The eggs are scheduled to travel directly to Australia or via Amsterdam, Doha, Dubai, Frankfurt, Hong 
Kong, Paris, and /or Singapore by air, or by a route approved by the Department of Agriculture, Water 
and the Environment in writing. The eggs are not scheduled to be accompanied in transit by other 
eggs or birds and are not scheduled to be transhipped unless approval for this has been given in 
writing by the Department of Agriculture, Water and the Environment, which I have sighted.   
  
Page  9 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  It is understood that in the event of a consignment arriving in Australia in an unsealed container, or in 
a container the seal of which has been broken, the consignment may not be permitted entry into 
Australia.  
Signature: .......................................................…….. Date: ........................................  
Government Approved Veterinarian or Official Veterinarian  
 
Name: … …………………………......................................................................................  
Address: ………………………….....................................................................................  
……………………………………........................................ ..................................................  
Email and contact number: ……………………………………..............................  
............................................................................................................................. ......... ....... 
   
  
Page  10 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Certificate: Health certificate 2 - Commercial chicken  
This health certificate relates to the post -collection observation, production records and disease status 
of the source flock of eggs for this consignment. The health certificate must be comp leted and signed 
by the Government Approved Veterinarian supervising the facility and the Official Veterinarian 
(Government veterinary officer employed by the government veterinary authority).  
Where options exist, the health certificate must indicate the option that applies.  
The completed health certificate must be sent to the biosecurity officer supervising the Government 
quarantine facility/AA site as soon as possible after the completion of the post egg collection 
observation period and test results ar e available.  
I. Identification of the consignment  
Department of Agriculture, Water and the Environment permit number: ………………………….  
Number of eggs  Mark(s)  Collection date(s)  
      
      
      
II. Origin of eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Date of export:  
IV. Health certification  
Part A: Flock status and disease testing. Certification by the Government Approved 
Veterinarian or Official Veterinarian supervising the source flock facility.  
I, ............................................................ ( name ), a Government Approved Veterinarian or Official 
Veterinarian* of ........................................................ ..... (country of export ) hereby certify in relation to 
the consignment of hatching eggs of poultry identified on this health certificate that:  
1. The source flock, from which the eggs were derived, has been under my direct supervision for the 
21 days sinc e the last day of collection of the eggs exported to Australia.  
2. Avian influenza  
 Not less than 14 days and not more than 21 days after the last day of collection of eggs for 
export to Australia, a sample of the source flock was tested serologically for i nfluenza virus 
type A with negative results.  
The sample tested was of a sufficient size to give a 99% confidence of detecting the disease if 
there was a 5% disease prevalence in the source flock.  
Total number of birds in the source flock: ........…………..... ...... 
Total number of birds tested: ........……………………….........…......  
3.  Newcastle disease (APMV -1) and paramyxovirus 3 (APMV -3) 
Vaccinated flocks only:   
  
Page  11 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   At least 14 days and not more than 21 days after the last day of collection of eggs for export to 
Australia, the same 100 individually identified birds that were tested pre -collection were re -
tested serologically for Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3). 
The individual titres are attached to the health certificate and are conside red consistent with 
vaccination against Newcastle disease virus (APMV -1) because:  
Either:  
o No bird showed a rise in titre of more than 2 dilutions over the first test, for both 
Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3) 
or 
o One or more bi rds showed a rise in titre of more than 2 dilutions over the first (for either 
Newcastle disease virus or paramyxovirus 3). An explanation as to why this is 
considered to be consistent with vaccination against Newcastle disease virus (APMV -
1) is attached t o the health certificate along with supporting evidence  
or 
o One or more birds showed a rise in titre of more than 2 dilutions over the first and virus 
isolation tests were performed with negative results. The individual virus isolation 
results are attached to the health certificate.  
OR 
 At least 14 days after the last collection of eggs for this consignment, the same unvaccinated 
sentinel birds which were tested pre -collection, were re -tested serologically for antibody to 
Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3) with negative results. Daily 
observation of these birds has been undertaken since introduction with no evidence of clinical 
signs of Newcastle disease (APMV -1) or paramyxovirus 3 (APMV -3) infection.  
Unvaccinated flocks:  
 Not less t han 14 days and not more than 21 days after the last day of collection of eggs for 
export to Australia, a sample of the source flock was tested serologically for Newcastle 
disease virus (APMV -1) with negative results. Testing was conducted on a flock sampl e of a 
sufficient size to give a  99% confidence of detecting the disease if there was a 5% disease 
prevalence in the source flock . 
Note: Post egg collection testing for paramyxovirus type 3 is not required for unvaccinated 
flocks.  
[The health certificate m ust indicate the option that applies].  
4. Avian pneumovirus  
Vaccinated flocks only:  
 At least 14 days and not more than 21 days after the last day of collection of eggs for export to 
Australia, the same 100 individually identified birds that were tested pre -collection were re -
tested serologically for avian pneumovirus. The individual titre s are attached to the health 
certificate and are considered consistent with vaccination against avian pneumovirus because:  
Either:  
o No bird showed a rise in titre of more than 2 dilutions over the first test  
or 
o One or more birds showed a rise in titre of m ore than 2 dilutions over the first. An 
explanation as to why this is considered to be consistent with vaccination against avian 
pneumovirus is attached to the health certificate along with supporting evidence  
or  
  
Page  12 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  o One or more birds showed a rise in titre of  more than 2 dilutions over the first and virus 
isolation tests were performed with negative results. The individual virus isolation 
results are attached to the health certificate.  
5. All tests were:  
 carried out in a government laboratory; or a laboratory  approved by the government of the 
exporting country for this specific purpose, and agreed to by the Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 2) or tests agreed to by the Department  of Agriculture, Water 
and the Environment.  
Test results of post egg collection testing are shown in the table below. Laboratory results and 
veterinary report explaining any positive results must be attached to the health certificate and 
forwarded to the Department of Agriculture, Water and the Environment.  
Total number of birds in the source flock: ........................................  
Disease  Laboratory  Test 
used  No. of 
tests  No. of positive 
results  Details of any re -
testing  
Influenza A  
(Influenza virus type A)  
           
 
Newcastle disease (APMV -
1) 
           
 
APMV -3  
  
  
  
  
 
Avian pneumovirus   
  
  
  
  
 
6. Investigations have been performed for:  
 any clinical disease or deaths above 0.5% per week in the source flock or sentinels, or  
 drop in quantity, quality, or fertility/hatchability of the eggs produced by the source flock.  
A veterinary report explaining the findings and any relevant laboratory reports are attached.  
 
Signature: .......................................................……..  Date: ........................................  
Government Approved Veterinarian or Official Veterinarian  
 
Name: ……………………………......................................................................................  
Address: ………………………….....................................................................................  
……………………………………..........................................................................................  
Email and contact number: ……………………………………............... ...............   
  
Page  13 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  ............................................................................................................................. ................  
[Note: All pages are to be endorsed with an Official Stamp].  
 
Part B: Disease status of the country of origin. Certification by the Official Veterinarian.  
I, ...............................................................( name ), an Official Veterinarian of 
....................................................... ( certifying ministry or department ) hereby certify in relation to the 
consignment of hatching eggs of poultry identified on this health certificate that during the time from 
the last collection of eggs for export to Australia to the completion of testing for this health certificate:  
1.…………… …………. ( country of export ) remains officially free of notifiable highly pathogenic avian 
influenza.  
and 
Avian influenza has not been reported on the source flock premises or within a radius of 40 km of its 
location.  
or 
The flock(s) of origin are in a zone o r compartment approved by the Department of Agriculture, Water 
and the Environment.  
 
[The health certificate must indicate the option that applies].  
2. Newcastle disease (APMV -1) has not been reported on the source flock premises or within a radius 
of 40 k m of its location.  
3. After due enquiry, I am satisfied that the source flock has remained closed. Any clinical evidence of 
disease or deaths above 0.5% per week has been investigated and the results indicate that the 
following diseases have not occurred i n the source flock:  
 Avian influenza  
 Newcastle disease (APMV -1) 
 Avian paramyxovirus type 3 (APMV -3) 
 Fowl typhoid ( Salmonella  Gallinarum)  
 Pullorum disease ( Salmonella  Pullorum)  
 Salmonella  Enteritidis infection  
 Arizona disease ( Salmonella  Arizonae)  
 Avian pneu movirus  
 Infectious bursal disease  
 Mycoplasma  iowae infection  
 Runting/stunting syndrome  
Signature: .......................................................…….. Date: ........................................  
Official Veterinarian  
 
Name: ……………………………........... ...........................................................................  
Address: ………………………….....................................................................................  
............................................................................................................................. ................   
  
Page  14 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Email and contact number: ……………………………………..............................  
……………………………………..........................................................................................  
[Note: All pages are to be endorsed with an Official Stamp].  
   
  
Page  15 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Certificate: Health certificate 1 - Ducks  
This health certificate relates to the pre -collection and collection preparation of the source flock of 
eggs for this consignment and must accompany the consignment to Australia. The health certificate 
must be completed and signed by the owner/manager, the Government approved veterinarian 
supervising the facility and the Official Veterinarian (government veterinary officer employed by the 
government veterinary authority) must accompany the consignment of eggs to Australia.  
Where options exist, the health certificate must indicate the option that applie s. 
I. Identification of the consignment  
Import permit number: ………………………  
Number of eggs  Mark(s)  Collection date(s)  
      
      
      
II. Origin of the eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Address of post -arrival quarantine isolation facility:  
Nature and identification of transport:  
Number and type of containers:  
Identification of container seals:  
IV. Health Certification  
PART A: De claration by the owner or manager of the source flock  
I, .............................................................. ( name ), the owner/manager ( delete one ) of the source flock 
from which the eggs to be exported to Australia were derived, hereby declare  that: 
1. The source flock has not been vaccinated against avian influenza.  
2. The source flock:  
 has not been vaccinated against Newcastle disease (APMV -1) 
 
or 
 has been vaccinated against Newcastle disease (APMV -1). 
[The health certificate must indicate the option that applies].  
 
3. Duck virus hepatitis type 1 (duck hepatitis A virus):   
 The source flock has not been vaccinated against duck virus hepatitis type 1 (duck hepatitis A 
virus)   
  
Page  16 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   
or 
 The source flock has been vaccinated against duck hepatitis virus  type 1 more than 10 weeks 
prior to the commencement of pre -egg collection testing.  
[The health certificate must indicate the option that applies].  
 
4. The source flock has not been vaccinated against duck virus enteritis.  
5. The vaccination history for N ewcastle disease (APMV -1) and duck virus hepatitis type 1 (duck 
hepatitis A virus) of the source flock is as follows OR is attached to this health certificate. Vaccination 
schedule(s) of other diseases are attached.  
[The health certificate must indicate th e option that applies].  
Disease  Date(s) of 
Vaccination  Name and type of 
Vaccine  
 Newcastle disease (APMV -1)     
Duck virus hepatitis type 1 (duck hepatitis A 
virus)       
6. The eggs have been laid by a source flock established and maintained as an all -in-all-out flock, the 
youngest birds being not less than 32 weeks old when eggs were collected.  
 
Signature: ........................................................……… Date: .. ................................  
Owner/Manager  
Name: ......................................................................….…………………………..........  
Address: .....................................................................…………………………….......  
............. .........................................................…………………………..............................  
 
PART B: Flock status and disease testing. Certification by the Government Approved 
Veterinarian or Official Veterinarian supervising the source flock facilit y. 
I, ............................................................ ( name ), a government approved veterinarian or Official 
Veterinarian* of ............................................................. ( country of export ) hereby certify in relation to 
the c onsignment of hatching eggs of poultry identified on this veterinary health certificate that:  
[*the health certificate must indicate which option applies].  
1. The source flock, from which the eggs for export to Australia were derived, has been under my 
direct supervision for the 90 days immediately preceding the collection of eggs for this consignment 
and during collection, and, after due enquiry, I have no rea son to doubt the truth of the 
owner's/manager's declaration above.  
2. The source flock is housed in secure rodent -proof and bird -proof buildings within the primary 
breeding establishment. The primary breeding establishment is isolated by 400 metres from a ll other 
neighbouring poultry farms and/or poultry breeding establishments unless these are shown by testing 
to be of a health status equal to the source flock.  
3. All water supplied to the source flock is secure against contamination by wild birds. All bu ildings 
containing feed and feeding equipment for the source flock are also bird -proofed.  
4. A biosecurity program has been in place during the entire period under my direct supervision to 
minimise the introduction of disease. This included the use of dedi cated staff for the source flock,  
  
Page  17 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  movement control and disinfection of vehicles not dedicated to the source flock facility, such as those 
for waste removal, feed delivery and service personnel vehicles.  
5. After due enquiry I am satisfied that there has be en no epidemiological contact between the source 
flock and any premises on which clinical Newcastle disease (APMV -1), avian influenza, duck virus 
hepatitis or duck viral enteritis has occurred within six months prior to the commencement of egg 
collection u ntil despatch of this consignment.  
6. The source flock has been free from clinical signs of the following disease agents for the 90 day 
period prior to collection of the eggs and during egg collection and has not come into contact with any 
birds showing e vidence of these diseases:  
 Avian influenza  
 Newcastle disease virus (APMV -1) 
 Infectious bursal disease virus  
 Duck virus hepatitis type 1 (duck hepatitis A virus)  
 Duck virus hepatitis type 2 (duck astrovirus 1)  
 Duck virus hepatitis type 3 (duck astrovirus  2) 
 Duck virus enteritis  
 Salmonella Enteritidis  
 Salmonella Typhimurium DT104  
 Goose and Muscovy duck parvovirus (Muscovies only)  
 Reovirus infection of Muscovy ducks (Muscovies only)  
7. Avian influenza testing  
Testing for influenza virus type A was conducte d on a flock sample of a sufficient size to give 99% 
confidence of detecting the disease if there was a 5% disease prevalence in the source flock . 
7.1. Serology  
Not more than 21 days before the first day of collection of eggs for export to Australia, a sa mple of the 
source flock was tested serologically for influenza virus type A with negative results.  
7.2. Virus isolation  
Not more than 21 days before the first day of collection of eggs for export to Australia, cloacal swabs 
were collected from a sample o f birds in the source flock. Each sample and each bird was identified 
so that a second sample could be collected at a later stage from any specified bird. Cloacal swabs 
from groups of no more than five birds were pooled and tested for freedom from haemaggl utinating 
agents by direct inoculation of the allantoic cavity of 9 -11 day -old chick embryos with cloacal swabs. 
No avian influenza virus was isolated.  
 
8. Newcastle disease virus (APMV -1) 
8.1. Serology  
8.1.1. Unvaccinated flock: Not more than 21 days be fore the first day of collection of eggs for export 
to Australia, a sample of the source flock was tested serologically with negative results for Newcastle 
disease (APMV -1).  
The flock sample tested was of a sufficient size to give a 99% confidence of dete cting the disease if 
there was a 5% disease prevalence in the source flock . 
or 
8.1.2. Vaccinated flock: not more than 21 days before the first day of collection of eggs, a random 
sample of 100 individually identified birds in the source flock was tested fo r Newcastle disease 
(APMV -1). Serological titres for each bird are attached to the health certificate and are consistent with  
  
Page  18 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  vaccination against Newcastle disease (APMV -1). The laboratory report specifies the units used for 
the titires and at what value t hey are considered positive.  
After sampling, the 100 individually identified birds were replaced randomly throughout the source 
flock. Testing is scheduled to be repeated on the same individually identified birds not less than 14 
days after the collection of the last egg for this consignment, in accord ance with Health certificate 2 . 
 
[The health certificate must indicate the option that applies].  
 
8.2. Virus isolation  
Not more than 21 days before the first day of collection of eggs for export to Australia, cloacal swabs 
were collected from a sample of birds in the source flock. Each sample and each bird was identified 
so that a second sample could be collected at a later s tage from any specified bird. Cloacal swabs 
from groups of no more than five birds were pooled and tested for freedom from haemagglutinating 
agents by direct inoculation of the allantoic cavity of 9 -11 day -old chick embryos with cloacal swabs. 
No Newcastle  disease virus (APMV -1) was isolated.  
The flock sample tested was of a sufficient size to give a 99% confidence of detecting the disease if 
there was a 5% disease prevalence in the source flock . 
 
9. Duck virus hepatitis , duck virus enteritis, salmonellosi s, parvovirus and reovirus  
Not more than 21 days before the first day of collection of eggs for export to Australia, a sample of the 
source flock was tested serologically with negative results for the following disease agents:  
 Duck virus hepatitis type 1 ( duck hepatitis A virus) (if unvaccinated)  
 Duck virus hepatitis type 2 (duck astrovirus 1)  
 Duck virus hepatitis type 3 (duck astrovirus 2)  
 Duck virus enteritis  
 Salmonella Enteritidis*  
 Goose parvovirus (Muscovies only)  
 Reovirus infection of Muscovy ducks (Muscovies only)  
The flock sample was of sufficient size to give a 99% confidence of detecting the disease if there was 
a 0.5% disease prevalence in the source flock . 
 
*OR for Salmonella  Enteritidis: the source flock is housed in an establishment that is subject to 
regular salmonella surveillance by serological testing of birds and/or environmental sampling and 
salmonella culture under a government scheme recognised by the Department of Agriculture, Water 
and the Environment (Appendix 1). No serological or microbiological evidence of S. Enteriditis was 
detected in the birds or environment.  
Name of scheme/s as listed in the government scheme recognised by the Department of Agriculture, 
Water and the Environment (Appendix 1): 
……………………........................................................................................................  
 
Accreditation number/s: ……………………..................................................  
 
Last two sample dates: …………… ……….........../.......................................  
10. All tests were:   
  
Page  19 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   carried  out in a government laboratory or a laboratory approved by the government of the 
exporting country for this specific purpose and agreed to by the Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 3), or tests agreed to by the Department of Agriculture, Water 
and the Environment.  
Test results are shown in the table below. Laboratory reports must also be attached to the health 
certificate and forwarded to the Department of Agriculture, Water and the Environment.  
Total number of birds in the source flock………………………..  
Disease  Laboratory  Test 
used  No. of 
tests  No. of 
positive 
results  Details of 
any re -
testing  
Avian influenza  
(Influenza virus type A)           
 
Newcastle disease virus (APMV -1)          
 
Duck virus hepatitis type 1 (duck hepatitis 
A virus) (if unvaccinated)           
 
Duck virus hepatitis type 2 (duck astrovirus 
1)          
 
Duck virus hepatitis type 3 (duck astrovirus 
2)          
 
Duck virus enteritis           
 
Salmonella  Enteritidis           
 
Goose parvovirus (Muscovies only)           
 
Reovirus of Muscovy ducks (Muscovies 
only)           
 
11. The eggs for export to Australia were collected over a period of no more than 14 days.  The eggs 
for export to Australia were collected separately to floor and dirty eggs.   No floor or dirty eggs are 
included in this consignment of eggs for export to Austr alia. 
12. The eggs in this consignment were clean and were not washed or cleaned after collection.  
13. The eggs were collected, indelibly marked and dispatched under the supervision of a government 
veterinary officer of the country of export.   
  
Page  20 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  14. After co llection, the eggs in this consignment were stacked on new egg flats (or egg flats that 
were cleaned and disinfected according to the requirements in this clause prior to use) to permit air 
circulation and, within eight hours of lay, were either:  
 fumigated  with formaldehyde gas in accordance with the OIE recommendations for the 
sanitation of hatching eggs and hatchery equipment.  
 
or 
 disinfected using Virkon  
 
or 
 disinfected using an agent approved by the Department of Agriculture, Water and the 
Environment. Written approval must be attached to the health certificate.  
 
Agent: ..........................................................................................................  
[The health certificate must indicate the option that applies].  
15. After fumiga tion/ disinfection, eggs were cooled to storage temperature and then packaged in the 
room they were fumigated/ disinfected into new crates with new, unused separators and sealed in 
leak-proof egg boxes for transport to Australia.  
 The eggs were handled and packed in a manner to avoid any contamination.  
 The sealed boxes were secured in isolation from other birds and eggs until dispatch.  
 To prevent any leakage if damage to the eggs occurs during transport either:  
 
- the sealed boxes were placed in plastic bags, sealed and packed into approved solid -sided 
aircraft containers.  
or 
- the sealed egg boxes were packed into approved solid -sided aircraft containers lined with 
plastic to prevent any leakage if damage to the  eggs occurs during transport.  
[The health certificate must indicate the option that applies].  
 
Signature: ........................................................……… Date: ..................................  
Government Approved Veterinarian or Official Ve terinarian  
 
Name: ......................................................................….…………………………..........  
Address: .....................................................................…………………………….......  
......................................................................…………………………..............................  
Email and contact number: ......................................................................………....  
[Note: All pages are to be endorsed with an official stamp].  
 
PART C: Disease status of the country of origin. Certification by the Official Veterinarian.  
I, ............................................................ ( name ), an Official Veterinarian of 
.......................................... ................... ( certifying ministry or department ) hereby certify in relation to 
the consignment of hatching eggs of poultry identified on this health certificate that:  
1. Avian influenza  
Either   
  
Page  21 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   The country of export is officially free of notifiable highly pathogenic avian influenza type A 
infection in commercial poultry, game birds and pet birds.  
 
and 
 No case of clinical avian influenza has occurred in poultry at the premises of origin or within a 
radius of 40 km thereof during the period commencing  21 days prior to the start of collection of 
eggs for this consignment until today.  
 
or 
 The flock(s) of origin are in a zone or compartment approved by the Department of Agriculture, 
Water and the Environment.  
2. Newcastle disease  
No case of Newcastle dis ease (APMV -1) has occurred in poultry at the premises of origin or within a 
radius of 40 km thereof during the period commencing 21 days prior to the start of collection of eggs 
for this consignment until today.  
Signature: ................................. .......................……… Date: ..................................  
Official Veterinarian  
 
Name: ......................................................................….…………………………..........  
Address: ......................................................... ............…………………………….......  
......................................................................…………………………..............................  
Email and contact number: ......................................................................………........  
 
PART D: Sealing approved aircraft containers at the airport and travel to Australia. 
Certification by the Government Approved Veterinarian or Official Veterinarian.  
I, the undersigned Official Veterinarian, certify that with respect to the hatching eggs of pou ltry 
described in this health certificate number ……………………………………………………, the 
individually sealed boxes containing the eggs were loaded onto solid -sided airline approved aircraft 
container(s) which were sealed with official government seals.  
Identification nu mbers of official government seals on aircraft containers (numbers) are: 
........................................................………  
I have checked the flight schedule/airway bill and can confirm that:  
The eggs are scheduled to travel directly to Australia  or via Amsterdam, Doha, Dubai, Frankfurt, Hong 
Kong, Paris and/or Singapore by air, or by a route approved by the Department of Agriculture, Water 
and the Environment in writing. The eggs are not scheduled to be accompanied in transit by other 
eggs or bir ds and are not scheduled to be transhipped unless approval for this has been given in 
writing by the Department of Agriculture, Water and the Environment, which I have sighted.  
It is understood that in the event of a consignment arriving in Australia in a n unsealed container, or in 
a container the seal of which has been broken, the consignment may not be permitted entry into 
Australia.  
 
Signature: ........................................................……… Date: ..................................  
Government Approved Veterinarian or Official Veterinarian  
 
Name: ......................................................................….…………………………..........   
  
Page  22 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Address: .....................................................................…………………………….......  
......................................................................…………………………..............................  
Email and contact number: ......................................................................………....  
   
  
Page  23 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Certificate: Health certificate 2 - Ducks  
This health certificate relates to the post -collection observation, production records and disease status 
of the source flock of eggs for this consignment. The health certificate must be completed and signed 
by the Government approved veterinarian su pervising the facility and the Official Veterinarian 
(government veterinary officer employed by the government veterinary authority).  
Where options exist, the health certificate must indicate the option that applies.  
The completed health certificate must be sent to the biosecurity officer supervising the government 
quarantine facility/AA site as soon as possible after the completion of the post egg collection 
observation period and test results are available.  
I. Identification of the consignment  
Department  of Agriculture, Water and the Environment permit number: ………………………….  
Number of eggs  Mark(s)  Collection date(s)  
      
      
      
II. Origin of eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Date of export:  
IV. Health certification  
PART A: Flock status and disease testing. Certification by the Government Approved 
Veterinarian or Official Veterinarian supervising the source flock facility.  
I, ............................................................ ( name ), a Government Approved Veterinarian or Official 
Veterinarian* of ............................................................. ( country of export ) hereby certify in relation to 
the cons ignment of hatching eggs of poultry identified on this health certificate that:  
1. The source flock, from which the eggs were derived, has been under my direct supervision for the 
21 days since the last day of collection of the eggs exported to Australia.  
2. Avian Influenza  
At least 14 days and not more than 21 days after the last day of collection of eggs for export to 
Australia, cloacal swabs were collected from a sample of birds in the source flock. The sample tested 
was of a sufficient size to give a 99% confidence of det ecting the disease if there was a 5% disease 
prevalence in the source flock . 
Each sample and each bird was identified so that a second sample could be collected at a later stage 
from any specified bird.  
Cloacal swabs from groups of no more than five birds were pooled and tested for freedom from 
haemagglutinating agents by direct inoculation of the allantoic cavity of 9 -11 day -old chick embryos. 
No avian influenza virus was isolated.  
3. Newcastle disease (APMV -1) 
a. Serology (Vaccinated source flock only):   
  
Page  24 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  At least 14 days and not more than 21 days after the last day of collection of eggs for export to 
Australia, the same 100 individually identified birds that were tested pre -collection were re -tested 
serologically for Newcastle disease virus (APMV -1). The individual titres are attached to the health 
certificate and are considered consistent with vaccination against Newcastle disease virus (APMV -1) 
because:  
Either:  
 No bird showed a rise in titre of more t han 2 dilutions over the first test, for both Newcastle 
disease virus (APMV -1) 
 
or 
 One or more birds showed a rise in titre of more than 2 dilutions over the first (for Newcastle 
disease virus). An explanation as to why this is considered to be consistent with vaccination 
against Newcastle disease virus (APMV -1) is attached to the health certificate along with 
supporting evidence  
 
or 
 One or more birds showed a rise in titre of more than 2 dilutions over the first and virus 
isolation tests were performed wit h negative results. The individual virus isolation results are 
attached to the health certificate.  
AND  
b. Virus isolation (vaccinated and unvaccinated source flocks):  
At least 14 days and not more than 21 days after the last day of collection of eggs, cloa cal swabs 
were collected from a sample of birds in the source flock. Each sample and each bird was identified 
so that a second sample could have been collected at a later stage from any specified bird. Cloacal 
swabs from groups of no more than five birds w ere pooled and tested for freedom from 
haemagglutinating agents by direct inoculation of the allantoic cavity of 9 -11 day -old chick embryoys. 
No Newcastle disease virus (APMV -1) was isolated.  
The sample tested was of a sufficient size to give a 99% confide nce of detecting the disease if there 
was a 5% disease prevalence in the source flock . 
4. All tests were  
 carried out in a government laboratory or a laboratory approved by the government of the 
exporting country for this specific purpose and agreed to by t he Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 3), or tests agreed to by the Department of Agriculture, Water 
and the Environment.  
Test results are shown in the table below. Laborator y reports must also be attached to the health 
certificate and forwarded to the Department of Agriculture, Water and the Environment.  
Total number of birds in the source flock………………………..  
Disease  Laboratory  Test 
used  No. of 
tests  No. of positive 
results  Details of any re -
testing  
Avian influenza (Influenza 
virus type A)         
   
Newcastle disease (APMV -1)            
  
Page  25 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   
5. Investigations have been performed for:  
 any clinical disease or deaths above 0.5% per week in the source flock, or sentinels, or  
 drop in quantity, quality, or fertility/hatchability of the eggs produced by the source flock.  
A veterinary report explaining the findings and any relevant laboratory results are attached.  
 
Signature: ........................................................………  Date: ..................................  
Government Approved Veterinarian or Official Veterinarian  
Name: ......................................................................….…………………………..........  
Address: ................................................ .....................…………………………….......  
......................................................................…………………………..............................  
Email and contact number: ............................................................................  
............................................................................................................................... ...........  
[NOTE:   All pages are to be endorsed with the Official Stamp].  
PART B: Disease status of the country of origin. Certifi cation by the Official Veterinarian.  
I, ............................................................ ( name ), an Official Veterinarian of 
............................................................. (certifying ministry or department) hereby certify in rel ation to 
the consignment of hatching eggs of poultry identified on this health certificate that during the time 
from the last collection of eggs for export to Australia to the completion of testing for this health 
certificate:  
1. ………………….. ( country of expo rt) remains officially free of highly pathogenic avian influenza  
and 
Avian influenza has not been reported on the source flock premises or within a radius of 40 km of its 
location.  
or 
The flock(s) of origin are in a zone or compartment approved by the Depa rtment of Agriculture, Water 
and the Environment.  
2. Newcastle disease (APMV -1) has not been reported on the source flock premises or within a radius 
of 40 kilometres of its location.  
3. After due enquiry, I am satisfied that the source flock has remained  closed. Any clinical evidence of 
disease or deaths above 0.5% per week has been investigated and the results indicate that the 
diseases below have not occurred in the source flock:  
 Avian influenza  
 Newcastle disease virus (APMV -1) 
 Infectious bursal disea se virus  
 Duck virus hepatitis type 1 (duck hepatitis A virus)  
 Duck virus hepatitis type 2 (duck astrovirus 1)  
 Duck virus hepatitis type 3 (duck astrovirus 2)  
 Duck virus enteritis   
  
Page  26 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   Salmonella Enteriditis  
 Salmonella Typhimurium DT104  
 Goose and Muscovy duck parvovirus (Muscovies only)  
 Reovirus infection of Muscovy ducks (Muscovies only)  
Signature: ........................................................……… Date: ..................................  
Official Veterinarian  
Name: ......................................................................….…………………………..........  
Address: .....................................................................…………………………….......  
.................................................................. ....…………………………..............................  
Email and contact number: ............................................................................  
............................................................................................................ .............................  
   
  
Page  27 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Certificate: Health certificate 1 - Turkey  
This health certificate relates to the pre -collection and collection preparation of the source flock of 
eggs for this consignment and must accompany the consignment to Australia.  The health certificate 
must be completed and signed by the owner/manager, the Government approved veterinarian 
supervising the facility and the Official Veterinarian (Government veterinary officer employed by the 
government veterinary authority) and must acc ompany the consignment of eggs to Australia.  
Where options exist, the health certificate must indicate the option that applies.  
I. Identification of the consignment  
Import permit number: ………………………  
Number of eggs  Mark(s)  Collection date(s)  
      
      
      
II. Origin of the eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Address of post -arrival quarantine isolation facility:  
Nature and identification of transport:  
Number and type of containers:  
Identification of container seals:  
IV. Health Certification  
Part A. Declaration by the owner or manager of the source flock  
I, .............................................................. ( name ), the owner/manager ( delete one ) of the source flock 
from which the eggs to be exported to Australia were derived, hereby declare that:  
1. The source flock has not been vaccinated against avian influenza.  
2. The source flock:  
 has not been vaccinated again st Newcastle disease (APMV -1) 
 
or 
 has been vaccinated against Newcastle disease (APMV -1). 
[The health certificate must indicate the option that applies].  
3. The source flock:  
 has not been vaccinated against avian pneumovirus  
 
or  
  
Page  28 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   has been vaccinated against  avian pneumovirus.  
[The health certificate must indicate the option that applies].  
4.  The vaccination history of the source flock for Newcastle disease (APMV -1) and avian 
pneumovirus is as follows OR is as attached to this health certificate. Vaccinatio n schedule(s) of other 
diseases are attached.  
[The health certificate must indicate the option that applies].  
Disease  Date(s) of Vaccination  Name and type of vaccine  
 Newcastle disease (APMV -1)  
   
 Avian pneumovirus   
   
5. The eggs have been laid by a source flock established and maintained as an all -in-all-out flock, the 
youngest birds being not less than 32 weeks old when eggs were collected.  
Signature: ........................................................……… Date: ................................. . 
Owner/Manager  
Name: ......................................................................….…………………………..........  
Address: .....................................................................…………………………….......  
............................................ ..........................…………………………..............................  
Part B: Flock status and disease testing. Certification by the Government Approved 
Veterinarian or Official Veterinarian supervising the source flock facility.  
I, .......................... .................................. ( name ), a Government Approved Veterinarian or Official 
Veterinarian* of ............................................................. ( country of export ) hereby certify in relation to 
the consignment of hatching eggs of p oultry identified on this health certificate that:  
[*the health certificate must indicate which option applies].  
1. The source flock, from which the eggs to be exported to Australia were derived, has been under my 
direct supervision for the 90 days immediately preceding the collection of eggs for this consignment 
and during collection, and, after due enquiry, I have no reason to doubt the truth of the 
owner's/manager's declaration above.  
2. The source flock is housed in secure rodent -proof and bird -proof buildings within the primary 
breeding establishment. The primary breeding establishment is isolated by 400 metres from all other 
neighbouring poultry farms and/or poultry breeding establishments unless these are shown by testing 
to be of a health stat us equal to the source flock.  
3. All water supplied to the source flock is secure against contamination by wild birds. All buildings 
containing feed and feeding equipment for the source flock are also bird -proofed.  
4. A biosecurity program has been in pla ce during the entire period under my direct supervision to 
minimise the introduction of disease.   This included the use of dedicated staff for the source flock, 
movement control and disinfection of vehicles not dedicated to the source flock facility, such as those 
for waste removal, feed delivery and service personnel vehicles.    
5. After due enquiry I am satisfied that there has been no epidemiological contact between the source 
flock and any premises on which clinical Newcastle disease (APMV -1) or avian i nfluenza has 
occurred from 90 days prior to the commencement of egg collection until despatch of this 
consignment.   
  
Page  29 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  6. The source flock has been free from clinical signs of the following diseases for the 90 day period 
prior to collection of the eggs and dur ing egg collection for export to Australia and has not come into 
contact with any birds showing evidence of these diseases:  
 Avian influenza  
 Newcastle  disease (APMV -1) 
 Avian paramyxovirus type 3 (APMV -3)  
 Fowl typhoid ( Salmonella  Gallinarum)  
 Pullorum  disease ( Salmonella  Pullorum)  
 Salmonella  Enteritidis infection  
 Arizona disease ( Salmonella Arizonae ) 
 Avian pneumovirus  
 Infectious bursal disease  
 Mycoplasma  iowae infection  
 Runting/stunting syndrome  
 Turkey lymphoproliferative disease  
 Turkey meningoencepha litis 
 Turkey viral hepatitis  
7. Avian influenza testing  
Not more than 21 days before the first day of collection of eggs for export to Australia, a sample of the 
source flock was tested serologically for influenza virus type A with negative results.  
The sa mple tested for influenza virus type A was of a sufficient size to give a 99% confidence of 
detecting the disease if there was a 5% disease prevalence in the source flock.  
8. Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3) serology  
Vaccinated  flocks:  
 Not more than 21 days prior to the first day of collection of eggs for export to Australia, a 
random sample of 100 individually identified birds in the source flock was tested for Newcastle 
disease virus (APMV -1) and paramyxovirus 3 (APMV -3). Sero logical titres for each bird are 
attached to the health certificate and are consistent with vaccination against Newcastle 
disease virus (APMV -1). The laboratory report specifies the units used for the titres and at 
what value they are considered positive.  
After sampling, the 100 individually identified birds were replaced randomly throughout the 
source flock. Testing is scheduled to be repeated on the same individually identified birds not 
less than 14 days after the collection of the last egg for this cons ignment, in accordance with 
Health Certificate 2.  
 
or 
 At least 14 days prior to the commencement of egg collection for this consignment, 
unvaccinated sentinel birds were placed evenly throughout the source flock at a rate of 1 
sentinel bird per 100 source flock birds with a minimum of 30 sentinel birds.   Daily observation 
of these birds has been undertaken with no evidence of clinical signs of Newcastle disease 
virus (APMV -1) or paramyxovirus 3 (APMV -3) infections. On the first day of collection of eggs 
for this consignment, each sentinel bird was tested serologically for Newcastle disease  
  
Page  30 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  (APMV -1) virus and paramyxovirus 3 (APMV -3) with negative results. Testing is scheduled to 
be repeated on the same sentinel birds not less than 14 days after the collectio n of the last 
egg for this consignment, in accordance with Health Certificate 2.  
Unvaccinated flocks:  
 Not more than 21 days before the first day of collection of eggs for export to Australia, a 
sample of the source flock was tested serologically for Newcastle disease (APMV -1) virus and 
paramyxovirus 3 (APMV -3) with negative results. Testing was conducted o n a flock sample of 
a sufficient size to give a  99% confidence of detecting the disease if there was a 5% disease 
prevalence in the source flock . 
[The health certificate must indicate the option that applies].  
9. Mycoplasma iowae  
Not more than 21 days before the first day of collection of eggs for export to Australia, all toms used 
for the artificial insemination of the source flock and the hens in the source flock were determined to 
be free of infection with Mycoplasma  iowae  by th e following methods.  
 Semen from each tom used for the artificial insemination of the source flock was cultured 
individually and found negative for Mycoplasma iowae .  
 
or 
 Phallus swabs from each tom used for the artificial insemination of the source flock w ere 
tested by specific polymerase chain reaction (PCR) test in pools of no more than 5 birds with 
negative results.   If any samples were positive, samples from all birds making up the pool 
were cultured individually and the results were negative for Mycopl asma  iowae . 
AND  
 Records of regular monitoring of dead -in-shell embryos, pipped eggs, etc. of the source flock 
were examined, and all available information indicates that the flock is free from 
Mycoplasma  iowae .   
 
or 
 All females in the source flock were te sted by culture for Mycoplasma  iowae and found to be 
free from the organism.  
 
or 
 All females in the source flock were tested by PCR using cloacal swabs in pools of no more 
than 5 birds with negative results.  
[The health certificate must indicate the option  that applies].  
10. Salmonella serology  
 The source flock is housed in an establishment that is subject to regular salmonella 
surveillance by serological testing of birds and/or environmental sampling and salmonella 
culture under a government scheme recogni sed by the Department of Agriculture, Water and 
the Environment (Appendix 1). No serological or microbiological evidence of S. Pullorum, S. 
Gallinarum or S. Enteritidis was detected in the birds or environment.  
Name of schem e/s as listed in the government scheme recognised by the Department of 
Agriculture, Water and the Environment (Appendix 1):  
  
Page  31 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   
……………………........................................................................................................  
 
Accreditation number/s: ……………………..................................................  
 
Last two sample dates: …………………….........../............... ........................  
 
or 
 Not more than 21 days before the first day of collection of eggs for export to Australia, a 
sample of the source flock was tested serologically for  S. Pullorum, S. Gallinarum or S. 
Enteriditis with negative results.  
The flock s ample tested was of a sufficient size to give a 99% confidence of detecting the disease if 
there was a 0.5% disease prevalence in the source flock . All birds that gave a positive or suspicious 
serological result for  S. Pullorum, S. Gallinarum or S. Enterid itis were killed and their organs cultured 
and identified for these  Salmonella  species.   The results of the cultures and identifications are 
attached and none of these species was identified.  
[The health certificate must indicate the option that applies].  
11. Salmonella Arizonae  
 The source flock is housed in an establishment that is subject to regular environmental 
sampling and salmonella culture under a government scheme recognised by the Department 
of Agriculture, Water and the Environment (Appendix 1) as being equivalent to the testing 
requirements. Salmonella Arizonae  species were not detected in the flock or the establishment 
during the previous two samplings.  
Name of scheme/s as listed in the government scheme recognised  by the Department of 
Agriculture, Water and the Environment (Appendix 1): 
 
……………………........................................................................................................  
 
Accreditation number/s: …………………….. ................................................  
 
Last two sample dates: …………………….........../.......................................  
 
or 
 Not more than 21 days before the first day of collection of eggs for export to Australia the 
source flock was determined to be free of infection with Salmonella Arizonae  by culture and 
isolation from shed litter.  
 
The total number of samples cultured was:  ……………………..  
20 samples, representative of the entire source flock, were collected. Each sample must be a 
composite sample of 3 floor and 2 nest litter samples; i.e. a total of 60 floor locations and 40 
nest box locations  
[The health certificate must indicate the option that applies].  
12.  Avian pneumovirus  
For flocks NOT vaccinated against avian pneumovirus:  
Not more than 21 days prior to the first day of collection of eggs for export to Australia, the source 
flock was tested using an ELISA test for a vian pneumovirus.   
  
Page  32 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  The sample tested was of a sufficient size to give a 99% confidence of detecting the disease if there 
was a 0.5% disease prevalence in the source flock . 
Number of Samples………….  
Either:  
 All results were negative  
 
or 
 Any birds returning pos itive results were re -tested using the IFA test with negative results. 
Test results must be attached to the health certificate. A short explanation must be provided 
by the certifying vet as to why these re -tests confirm the positive results as false: 
…………… ………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
………………………………………………………………….  
For flocks vaccinated against avian pneumovirus : 
Not more than 21 days prior to the first day of collection of  eggs for export to Australia, a random 
sample of 100 individually identified birds in the source flock were serologically tested for avian 
pneumovirus using the ELISA method.  
Test results for each bird are attached to the health certificate and are consi stent with vaccination 
against avian pneumovirus. The laboratory report specifies the units used for the titres and at what 
value they are considered positive. After sampling, the 100 individually identified birds were replaced 
randomly throughout the sour ce flock. Testing is scheduled to be repeated on the same individually 
identified birds not less than 14 days after the collection of the last egg for this consignment, in 
accord ance with Health certificate 2 . 
[The health certificate must indicate the opti on that applies].  
 
13. All tests were:  
 carried out in a government laboratory; or a laboratory approved by the government of the 
exporting country for this specific purpose, and agreed to by the Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 2), or tests agreed to by the Department of Agriculture, Water 
and the Environment.  
Test results are shown in the table below. Laboratory reports must also be attached to the health 
certificate and forw arded to the Department of Agriculture, Water and the Environment.  
Total number of birds in the source flock: ………………………………….  
Disease agent  Laboratory  Test 
used  No. of 
tests  No. of positive 
results  Details of any re -
testing  
Avian Influenza (Influenza 
virus type A)   
        
 
Newcastle disease virus 
(APMV -1)  
        
 
APMV -3       
  
Page  33 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry       
Mycoplasma  iowae   
        
 
Salmonella  Pullorum   
        
 
Salmonella  Gallinarum   
        
 
Salmonella  Enteritidis   
        
 
Salmonella Arizonae   
        
 
Avian pneumovirus   
  
  
  
  
 
14.  The eggs for export to Australia were collected over a period of no more than 14 days.  The eggs 
for export to Australia were collected separately to floor and dirty eggs.   No floor or dirty eggs are 
included in this consignment of eggs for export to Australia.  
15.  The eggs in this consignment were clean and were not washed or cleaned after collection.  
16.  The eggs were collected, indelibly marked and dispatched under the supervision of a 
Government Veterinary Officer of the cou ntry of export.  
17. After collection, the eggs in this consignment were stacked on new egg flats (or egg flats that 
were cleaned and disinfected according to the requirements in this clause, prior to use) to permit air 
circulation and, within eight hours o f lay, were either:  
 fumigated with formaldehyde gas in accordance with the OIE recommendations for the 
sanitation of hatching eggs and hatchery equipment.  
 
or 
 disinfected with Virkon.  
 
or 
 disinfected  using an agent approved by the Department of Agriculture, Water and the 
Environment. Written approval must be attached to the health certificate.  
 
Agent:...................................................................................................... ................  
[The health certificate must indicate the option that applies].  
18. After fumigation/ disinfection, eggs were cooled to storage temperature and then packaged in the 
room they were fumigated/ disinfected into new crates with new, unused separators and sealed in 
leak-proof egg boxes for transport to Australia.  
 The eggs were handled and packed in a manner to avoid any contamination.   
  
Page  34 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   The sealed boxes were secured in isolation from other birds and eggs until dispatch.  
 To prevent any leakage if damage to the eggs occurs during transport either:  
- the sealed egg boxes were placed in plastic bags, sealed and packed into approved solid -
sided aircraft containers  
or 
- the sealed egg boxes were packed into approved solid -sided aircraft containers li ned with 
plastic to prevent any leakage if damage to the eggs occurs during transport.  
Signature: ........................................................……… Date: ..................................  
Government Approved Veterinarian or Official Veterinarian  
Name: ......................................................................….…………………………..........  
Address: .....................................................................……………………………........  
.......................................................... ............…………………………...............................  
Email and contact number: ......................................................................……….......................... ....... 
[NOTE: All pages  are to be endorsed with the Official Stamp].  
Part C:  Disease status of the country of origin. Certification by the Official Veterinarian.  
I, ............................................................ ( name ), an Official Veterinarian of 
............................................................. ( certif ying ministry or department ) hereby certify in relation to 
the consignment of hatching eggs of poultry identified on this health certificate that:  
1.  Avian influenza  
Either:  
 The country of export is officially free of notifiable highly pathogenic avian i nfluenza type A 
infection in commercial poultry, game birds and pet birds.  
 
and 
 No case of clinical avian influenza has occurred in poultry at the premises of origin or within a 
radius of 40 km thereof during the period commencing 21 days prior to the star t of collection of 
eggs for this consignment until today.  
 
or 
 The flock(s) of origin are in a zone or compartment approved by the Department of Agriculture, 
Water and the Environment.  
[The health certificate must indicate the option that applies].  
2. Newcastle disease  
No case of clinical Newcastle disease (APMV -1) has occurred in poultry at the premises of origin or 
within a radius of 40 km thereof during the period commencing 21 days prior to the start of collection 
of eggs for this consignment until today.  
 
Signature: ........................................................……… Date: ..................................  
Official Veterinarian  
Name: ......................................................................….…………………………..........  
Address: ...... ...............................................................…………………………….......  
......................................................................…………………………..............................   
  
Page  35 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Email and contact number: ..................................... .................................……….................  
Part D: Sealing approved aircraft containers at the airport and travel to Australia. Certification 
by the Government Approved Veterinarian or Official Veterinarian.  
I, the undersigned Official Veterina rian, certify that with respect to the hatching eggs of poultry 
described in this health certificate number ……………………………………………………, the 
individually sealed boxes containing the eggs were loaded onto solid -sided airline approved aircraft 
container(s) which we re sealed with official government seals.  
Identification numbers of official government seals on aircraft containers (numbers) are: 
........................................................………  
I have checked the flight schedule/airway bill and can confirm t hat: 
The eggs are scheduled to travel directly to Australia or via Amsterdam, Doha, Dubai, Frankfurt, Hong 
Kong, Paris and/or Singapore by air, or by a route approved by the Department of Agriculture, Water 
and the Environment in writing. The eggs are not scheduled to be accompanied in transit by other 
eggs or birds and are not scheduled to be transhipped unless approval for this has been given in 
writing by the Department of Agriculture, Water and the Environment, which I have sighted.  
It is understood that in the event of a consignment arriving in Australia in an unsealed container, or in 
a container the seal of which has been broken, the consignment may not be permitted entry into 
Australia.  
Signature: .................................................. ......……… Date: ..................................  
Government Approved Veterinarian or Official Veterinarian  
Name: ......................................................................….…………………………..........  
Address: ....................................... ..............................…………………………….......  
......................................................................…………………………..............................  
Email and contact number: ...................................................................... ……….............  
   
  
Page  36 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Certificate: Health certificate 2 - Turkey  
This health certificate relates to the post -collection observation, production records and disease status 
of the source flock of eggs for this consignment. The health certificate must be complet ed and signed 
by the Government approved veterinarian supervising the facility and the Official Veterinarian 
(Government veterinary officer employed by the government veterinary authority).  
Where options exist, the health certificate must indicate the opt ion that applies.  
The completed health certificate must be sent to the biosecurity officer supervising the Government 
quarantine facility/AA site as soon as possible after the completion of the post egg collection 
observation period and test results are av ailable.  
I. Identification of the consignment  
Department of Agriculture, Water and the Environment permit number: ………………………….  
Number of eggs  Mark(s)  Collection date(s)  
      
      
      
II. Origin of eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Date of export:  
IV. Health certification  
PART A: Flock status and disease testing. Certification by the Government Approved 
Veterinarian or Officia l Veterinarian supervising the source flock facility.  
I, ............................................................ ( name ), a Government Approved Veterinarian or Official 
Veterinarian* of ............................................................. ( country of export ) hereby certify in relation to 
the consignment of hatching eggs of poultry identified on this health certificate that:  
1. The source flock, from which the eggs were derived, has been under my direct supervision for the 
21 days since the l ast day of collection of the eggs exported to Australia.  
2. Avian influenza  
 Not less than 14 days and not more than 21 days after the last day of collection of eggs for 
export to Australia, a sample of the source flock was tested serologically for influenz a virus 
type A with negative results.  
The sample tested was of a sufficient size to give a 99% confidence of detecting the disease if 
there was a 5% disease prevalence in the source flock . 
Total number of birds in the source flock: ........…………...........  
Total number of birds tested: ........……………………….........…  
3. Newcastle disease (APMV -1) and paramyxovirus 3 (APMV -3) 
Vaccinated flocks only:   
  
Page  37 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   At least 14 days and not more than 21 days after the last day of collection of eggs for export to 
Australia, the sa me 100 individually identified birds that were tested pre -collection were 
retested serologically for Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3). 
The individual titres are attached to the health certificate and are considered consistent w ith 
vaccination against Newcastle disease virus (APMV) because:  
Either:  
o No bird showed a rise in titre of more than 2 dilutions over the first test, for both 
Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3) 
or 
o One or more birds showed a rise i n titre of more than 2 dilutions over the first (for either 
Newcastle disease virus or paramyxovirus 3). An explanation as to why this is 
considered to be consistent with vaccination against Newcastle disease virus (APMV -
1) is attached to the health certif icate along with supporting evidence  
or 
o One or more birds showed a rise in titre of more than 2 dilutions over the first and virus 
isolation tests were performed with negative results. The individual virus isolation 
results are attached to the health certi ficate.  
OR 
 At least 14 days after the last collection of eggs for this consignment, the same unvaccinated 
sentinel birds which were tested pre -collection, were re -tested serologically for antibody to 
Newcastle disease virus (APMV -1) and paramyxovirus 3 (AP MV-3) with negative 
results.   Daily observation of these birds has been undertaken since introduction with no 
evidence of clinical signs of Newcastle disease (APMV -1) or paramyxovirus 3 (APMV -3) 
infection.  
Unvaccinated flocks:  
 Not less than 14 days and not more than 21 days after the last day of collection of eggs for 
export to Australia, a sample of the source flock was tested serologically for Newcastle 
disease virus (APMV -1) with negative results. Testing was conducted on a f lock sample of a 
sufficient size to give a 99% confidence of detecting the disease if there was a 5% disease 
prevalence in the source flock . 
Note: Post egg collection testing for paramyxovirus type 3 is not required for unvaccinated 
flocks.  
[The health cer tificate must indicate the option that applies].  
4. Avian pneumovirus  
Vaccinated flocks only:  
 At least 14 days and not more than 21 days after the last day of collection of eggs for export to 
Australia, the same 100 individually identified birds that were tested pre -collection were 
retested serologically for avian pneumovirus. The individual titres are attached to the health 
certificate and are considered consistent with vaccination against avian pneumovirus because:  
Either:  
o No bird showed a rise in titre o f more than 2 dilutions over the first test  
or 
o One or more birds showed a rise in titre of more than 2 dilutions over the first test. An 
explanation as to why this is considered to be consistent with vaccination against avian 
pneumovirus is attached to the  health certificate along with supporting evidence  
or  
  
Page  38 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  o One or more birds showed a rise in titre of more than 2 dilutions over the first and virus 
isolation tests were performed with negative results. The individual virus isolation 
results are attached to th e health certificate.  
5. All tests were:  
 carried out in a government laboratory; or a laboratory approved by the government of the 
exporting country for this specific purpose, and agreed to by the Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 2), or tests agreed to by the Department of Agriculture, Water 
and the Environment.  
Test results are shown in the table below. Laboratory reports and veterinary report explaining any 
positive re sults  
Disease  Test used  Test used  No of tests  Details of any re -testing  
Influenza A  
(Influenza virus type A)   
       
Newcastle disease (APMV -1)   
       
APMV -3  
  
  
  
 
Avian pneumovirus   
  
  
  
 
6. Investigations have been performed for:  
 any clinical disease or deaths above 0.5% per week in the source flock or sentinels, or  
 drop in quantity, quality, or fertility/hatchability of the eggs produced by the source flock.  
A veterinary report explaining the findings and any relevant laboratory  results are attached.  
Signature: .......................................................…….. Date: ........................................  
Government Approved Veterinarian or Official Veterinarian  
 
Name: ……………………………....................................... ...............................................  
Address: ………………………….....................................................................................  
............................................................................................................................. ................  
Email and contact number: ……………………………………..............................  
……………………………………............................. .............................................................  
[Note: All pages are to be endorsed with an Official Stamp].  
 
PART B: Disease status of the country of origin. Certification by the official veterinarian.  
I, ...............................................................(  name ), an Official Veterinarian of 
....................................................... ( certifying ministry or department ) hereby certify in relation to the  
  
Page  39 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  consignment of hatching eggs o f poultry identified on this health certificate that during the time from 
the last collection of eggs for export to Australia to the completion of testing for this health certificate.  
1. ………………….( country of export)  remains officially free of notifiable hi ghly pathogenic avian 
influenza.  
and 
Avian influenza has not been reported on the source flock premises or within a radius of 40 km of its 
location.  
or 
The flock(s) of origin are in a zone or compartment approved by the Department of Agriculture, Water 
and the Environment.  
[The health certificate must indicate the option that applies].  
2. Newcastle disease (APMV -1) has not been reported on the source flock premises or within a radius 
of 40 km of its location.  
3. After due enquiry, I am satisfied that the so urce flock has remained closed. Any clinical evidence of 
disease or deaths above 0.5% per week has been investigated and the results indicate that the 
following diseases have not occurred in the source flock:  
 Avian influenza  
 Newcastle disease (APMV -1) 
 Avian paramyxovirus type 3 (APMV -3) 
 Pullorum disease ( Salmonella  Pullorum)  
 Fowl typhoid ( Salmonella  Gallinarum)  
 Salmonella  Enteritidis infection  
 Arizona disease ( Salmonella  Arizonae)  
 Avian pneumovirus infection  
 Infectious bursal disease  
 Mycoplasma  iowae  infect ion 
 Runting/stunting syndrome  
 Turkey lymphoproliferative disease  
 Turkey meningoencephalitis  
 Turkey viral hepatitis  
Signature: .......................................................…….. Date: ........................................  
Official Veterinarian  
 
Name: ……………………………......................................................................................  
Address: ………………………….....................................................................................  
............................................... ..............................................................................................  
Email and contact number: ……………………………………..............................  
……………………………………..........................................................................................   
  
Page  40 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Certificate: Health certificate 1 - Non-commercial fancy chickens  
This health certificate relates to the pre -collection and collection preparation of t he source flock of 
eggs for this consignment and must accompany the consignment to Australia. The health certificate 
must be completed and signed by the owner/manager, the Government approved veterinarian 
supervising the facility and the Official Veterinar ian (government veterinary officer employed by the 
government veterinary authority) and must accompany the consignment of eggs to Australia.  
Where options exist, the health certificate must indicate the option that applies.  
 
I. Identification of the consig nment  
Import permit number: ………………………  
Number of eggs  Mark(s)  Collection dates(s)  
      
      
      
II. Origin of the eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Address of post -arrival quarantine isolation facility:  
Nature and identification of transport:  
Number and type of containers:  
Identification of container seals:  
IV. Health Certification  
Part A. Declaration by the owner or manager of the source flock.  
I, .............................................................. ( name ), the owner/manager ( delete one ) of the source flock 
from which the eggs to be exported to Australia were derived, hereby declare that:  
1. The source flock has not  been vaccinated against avian influenza.  
2. The source flock has not been vaccinated against Newcastle disease (APMV -1) 
3. The source flock:  
 has not been vaccinated against avian pneumovirus  
 
or 
 has been vaccinated against avian pneumovirus.  
[The health certificate must indicate the option that applies].  
4. The vaccination history of the source flock for avian pneumovirus is as follows OR is attached to 
this health certificate. Vaccination schedule(s) of other diseases are attached.  
[The health certificat e must indicate the option that applies].   
  
Page  41 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Disease  Date(s) of vaccination  Name and type of vaccine  
 Avian pneumovirus      
 
5. The eggs have been laid by a source flock established and maintained as an all -in-all-out flock, the 
youngest birds being not less than 32 weeks old when eggs were collected.  
Signature: ........................................................……… Date: ...............................  
Owner/Manager  
Name: ......................................................................….…………………………......  
Address: .....................................................................…………………………….....  
......................................................................…… …………………….........................  
Part B: Flock status and disease testing. Certification by the Government Approved 
Veterinarian or Official Veterinarian supervising the source flock facility.  
I, ........................................................... . (name ), a Government Approved Veterinarian or Official 
Veterinarian* of ............................................................. ( country of export ) hereby certify in relation to 
the consignment of hatching eggs of poultry identified on this health certificate that:  
[*the health certificate must indicate which option applies].  
1. The source flock, from which the eggs for export to Australia were derived, has been under my 
direct supervision for the 90 days immediately preceding the collection of eggs  for this consignment 
and during collection, and, after due enquiry, I have no reason to doubt the truth of the 
owner's/manager's declaration above.  
2. The source flock is housed in secure rodent -proof and bird -proof buildings within the primary 
breeding e stablishment. The primary breeding establishment is isolated by 400 metres from all other 
neighbouring poultry farms and/or poultry breeding establishments unless these are shown by testing 
to be of a health status equal to the source flock.  
3. All water supplied to the source flock is secure against contamination by wild birds. All buildings 
containing feed and feeding equipment for the source flock are also bird -proofed.  
4. A biosecurity program has been in place during the entire period under my direct supervision to 
minimise the introduction of disease. This included the use of dedicated staff for the source flock, 
movement control and disinfection of vehicles not dedicated to the source flock facility, such as those 
for waste removal, feed delivery and  service personnel vehicles.  
5. After due enquiry I am satisfied that there has been no epidemiological contact between the source 
flock and any premises on which clinical Newcastle disease (APMV -1) or avian influenza has 
occurred from 90 days prior to th e commencement of egg collection until despatch of this 
consignment.  
6. The source flock has been free from clinical signs of the following diseases for the 90 day period 
prior to collection of the eggs and during egg collection for export to Australia and  has not come into 
contact with any birds showing evidence of these diseases.  
 Avian influenza  
 Newcastle disease (APMV -1) 
 Avian paramyxovirus type 3 (APMV -3) 
 Fowl typhoid ( Salmonella Gallinarum)  
 Pullorum disease ( Salmonella Pullorum)  
 Salmonella Enteritidis infection   
  
Page  42 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   Arizona disease ( Salmonella Arizona)  
 Avian pneumovirus  
 Infectious bursal disease  
 Mycoplasma  iowae  infection  
 Runting/stunting syndrome  
7. Avian influenza testing  
Not more than 21 days before the first day of collection of eggs for  export to Australia, a sample of the 
source flock was tested serologically for influenza virus type A with negative results.  
Testing for influenza virus type A must be conducted on a flock sample of a sufficient size to give a 
99% confidence of detecting the disease if there was a 5% disease prevalence in the source flock . 
8. Newcastle disease virus (APMV -1) and paramyxovirus 3 (APMV -3) serology  
Not more than 21 days before the first day of collection of eggs for export to Australia, a sample of the 
source flock was tested serologically for Newcastle disease virus (APMV -1) and paramyxovirus 3 
(APMV -3) with negative results.  
Testing was conducted on a flock sample of a sufficient size to give a 99% confidence of detecting the 
disease if there was a 5% disease prevalence in the source flock . 
9. Salmonella  serology  
 All birds on -site (sample = 100%) were tested at least twice for S. Pullorum and S. Gallinarum 
using the RSAT method. Testing was undertaken at least 21 days apart. The final test was 
undertake n within 21 days of the first day of collection of eggs for export.  
 
AND  
 Not more than 21 days before the first day of collection of eggs for export to Australia, a 
sample of the source flock was tested serologically using the ELISA method for S. Enteritid is 
with negative results. The flock sample tested was of a sufficient size to give a 99% 
confidence of detecting the disease if there was a 0.5% disease prevalence in the source 
flock. 
All birds that gave a positive or suspicious serological result for S. Pullorum, S. Gallinarum or S. 
Enteriditis were killed and their organs cultured and identified for these Salmonella  species. The 
results of the cultures and identifications are attached and none of these species were identified.  
10. Salmonella Arizonae  
 Not more than 21 days before the first day of collection of eggs for export to Australia the 
source flock was determined to be free of infection with Salmonella arizonae by culture and 
isolation from shed litter of all occupied sheds.  
The total number of samp les cultured was: ……………………..  
20 samples, representative of the entire source flock, were collected. Each sample must be a 
composite sample of 3 floor and 2 nest litter samples; i.e. a total of 60 floor locations and 40 
nest box locations.  
11. Salmonella  Pullorum and Gallinarum culture  
The source flock was determined to be free of infection with Salmonella  Pullorum and Gallinarum by 
the specific bacterial culture and isolation test from shed litter of all occupied sheds.   
  
Page  43 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   20 samples, representative of the e ntire source flock, were collected. Each sample was a 
composite sample of 3 floor and 2 nest litter samples; i.e. a total of 60 floor locations and 40 
nest box locations. Boot swabs or drag swabs are acceptable.  
 Sampling as above was at least twice, a mini mum of 14 days apart. The second sampling and 
culture must be within 21 days of the first egg collection.  
 The method used for culture is certified as suitable for the isolation of non -motile salmonellae 
including Salmonella  Pullorum  and Gallinarum.  
12. Avian pneumovirus  
For flocks NOT vaccinated against avian pneumovirus:  
No more than 21 days prior to the first day of collection of eggs for export to Australia, the source 
flock was tested using an ELISA test for avian pneumovirus.  
The sample tested was of a sufficient size to give a 99% confidence of detecting the disease if there 
was a 0.5% disease prevalence in the source flock . 
Number of samples: .........................  
Either:  
 All results were negative.  
 
or 
 Any birds returning positive results were re -tested using the IFA test with negative results. 
Test results must be attached to the health certificate. A short explanation must be  
provided by the certifying vet as to why these re -tests confirm the positive  results as false: 
............................................................................................................................. .........................
....................................................................................... ...............................................................
............................................................................................................................. .........................
........................................... ............................................................  
For flocks vaccinated against avian pneumovirus:  
Not more than 21 days prior to the first day of collection of eggs for export to Australia, a random 
sample of 100 individually identified birds i n the source flock were serologically tested for avian 
pneumovirus using the ELISA method.  
Test results for each bird are attached to the health certificate and are consistent with vaccination 
against avian pneumovirus. The laboratory report specifies the  units used for the titres and at what 
value they are considered positive. After sampling, the 100 individually identified birds were replaced 
randomly throughout the source flock. Testing is scheduled to be repeated on the same individually 
identified bir ds not less than 14 days after collection of the last egg for this consignment, in 
accord ance with Health certificate 2 . 
 
[The health certificate must indicate the option that applies].  
 
13. All tests were:  
 carried out in a government laboratory; or a labo ratory approved by the government of the 
exporting country for this specific purpose, and agreed to by the Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 2) or tests agreed to by the Depar tment of Agriculture, Water 
and the Environment.   
  
Page  44 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Test results are shown in the table below. Laboratory reports must also be attached to the health 
certificate and forwarded to the Department of Agriculture, Water and the Environment.  
Total number of birds in the source flock: ………………………………….  
Disease  Laboratory  Test 
used  No of 
tests  No of positives 
results  Details of any re -
testing  
Avian influenza (Influenza 
virus type A)         
   
Newcastle disease virus 
(APMV -1)        
   
APMV -3        
   
Salmonella Pullorum         
   
Salmonella Gallinarum         
   
Salmonella Enteritidis         
   
Salmonella Arizonae         
   
Avian pneumovirus         
   
14. The eggs for export to Australia were collected over a period of no more than 14 days. The eggs 
for export to Australia were collected separately to floor and dirty eggs. No floor or dirty eggs are 
included in this consignment of eggs for export to Australia.  
15. The eggs in this consignment were clean and were not washed or cleaned after collection.  
16. The eggs were collected indelibly marked and dispatched under the supervision of a Government 
Veterinary Officer of the country of export.  
17. After collection, the eggs in this consignment were stacked on new egg flats (or egg flats that 
were cleaned and  disinfected according to the requirements in this clause, prior to use) to permit air 
circulation and, within eight hours of lay, were either:  
 fumigated with formaldehyde gas in accordance with the OIE recommendations for the 
sanitation of hatching eggs a nd hatchery equipment.  
 
or 
 disinfected using Virkon  
 
or  
  
Page  45 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   disinfected using an agent approved by the Department of Agriculture, Water and the 
Environment. Written approval must be attached to the health certificate.  
 
Agent: .................................. ........................................................................  
[The health certificate must indicate the option that applies].  
18. After fumigation/ disinfection, eggs were cooled to storage temperature and then packaged in the 
room they were fum igated/ disinfected into new crates with new, unused separators and sealed in 
leak-proof egg boxes for transport to Australia.  
 The eggs were handled and packed in a manner to avoid any contamination.  
 The sealed boxes were secured in isolation from other bi rds and eggs until dispatch.  
 To prevent any leakage if damage to the eggs occurs during transport either:  
- the sealed egg boxes were placed in plastic bags, sealed and packed into approved solid -
sided aircraft containers  
or 
- the sealed egg boxes were packed into approved solid -sided aircraft containers lined with 
plastic to prevent any leakage if damage to the eggs occurs during transport.  
 
Signature: ........................................................……… Date: ..................................  
Government Approved Veterinarian or Official Veterinarian  
Name: ......................................................................….…………………………..........  
Address: .....................................................................…………………………….......  
......................................................................…………………………..............................  
Email and contact number: ............................................................................  
............................................. ............................................................................................  
[NOTE: All pages  are to be endorsed with the Official Stamp].  
Part C: Disease status of the country of origin. Certification by the Official Veterinarian.  
I, ............................................................ ( name ), an Official Veterinarian of 
.............................................................( certifying ministry or department ) hereby certify in relation to 
the consignment of hatching eggs  of poultry identified on this health certificate that:  
1. Avian influenza  
Either  
 The country of export is officially free of notifiable highly pathogenic avian influenza type A 
infection in commercial poultry, game birds and pet birds.  
 
and 
 No case of cli nical avian influenza has occurred in poultry at the premises of origin or within a 
radius of 40 km thereof during the period commencing 21 days prior to the start of collection of 
eggs for this consignment until today.  
 
or 
 The flock(s) of origin are in a zone or compartment approved by the Department of Agriculture, 
Water and the Environment.   
  
Page  46 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  [The health certificate must indicate the option that applies].  
 
2. Newcastle disease  
No case of clinical Newcastle disease (APMV -1) has occurred in poultry at the pr emises of origin or 
within a radius of 40 km thereof during the period commencing 21 days prior to the start of collection 
of eggs for this consignment until today.  
Signature: ........................................................……… Date: ............. ...................  
Official Veterinarian  
Name: ......................................................................….…………………………......  
Address: .....................................................................…………………………….......  
...................... ................................................………………………….....................  
Email and contact number: ............................................................................  
......................................................................... .......................................................  
Part D: Sealing approved aircraft containers at the airport. Certification by the Government 
Approved Veterinarian or Official veterinarian.  
I, the undersigned Official Veterinarian, certify that with respect to the hatching eggs of poultry 
described in this health certificate number ……………………………………………………, the 
individually sealed boxes containing the eggs were loaded onto solid -sided airline approved aircraft 
container(s) which were sealed with offi cial government seals.  
Identification numbers of official government seals on aircraft containers (numbers) are: 
........................................................………  
I have checked the flight schedule/airway bill and can confirm that:  
The eggs are s cheduled to travel directly to Australia via Amsterdam, Doha, Dubai, Frankfurt, Hong 
Kong, Paris and/or Singapore by air, or by a route approved by the Department of Agriculture, Water 
and the Environment in writing. The eggs are not scheduled to be accomp anied in transit by other 
eggs or birds and are not scheduled to be transhipped unless approval for this has been given in 
writing by the Department of Agriculture, Water and the Environment, which I have sighted.  
It is understood that in the event of a c onsignment arriving in Australia in an unsealed container, or in 
a container the seal of which has been broken, the consignment may not be permitted entry into 
Australia.  
Signature: ........................................................……… Date: ........ ..........................  
Government Approved Veterinarian or Official Veterinarian  
Name: ......................................................................….…………………………..........  
Address: ............................................................... ......…………………………….......  
......................................................................…………………………..............................  
Email and contact number: ............................................................................  
................. ........................................................................................................................  
   
  
Page  47 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Certificate: Health certificate 2 - Non-commercial fancy chickens  
This health certificate relates to the post -collection observation, production records and disease status 
of the source flock of eggs for this consignment. The health certificate must be completed and signed 
by the Government approved veterinarian supervis ing the facility and the Official Veterinarian 
(Government veterinary officer employed by the government veterinary authority).  
Where options exist, the health certificate must indicate the option that applies.  
The completed health certificate must be sen t to the biosecurity officer supervising the government 
quarantine facility as soon as possible after the completion of the post egg collection observation 
period and test results are available.  
I. Identification of the consignment  
Department of Agricultur e, Water and the Environment permit number: ………………………….  
Number of eggs  Mark(s)  Collection date(s)  
      
      
      
II. Origin of eggs  
Name and address of the source flock facility:  
Name and address of exporter:  
Country of export:  
III. Destination of the eggs  
Name and address of consignee:  
Date of export:  
IV. Health certification  
Part A: Flock status and disease testing. Certification by the Government Approved 
Veterinarian or Official Veterinarian supervising the source flock facility.  
I, ............................................................ ( name ), a Government Approved Veterinarian or Official 
Veterinarian of ............................................................. ( country of export ) hereby certify in relation to 
the consignm ent of hatching eggs of poultry identified on this health certificate that:  
1. The source flock, from which the eggs were derived, has been under my direct supervision for the 
21 days since the last day of collection of the eggs exported to Australia.  
2. Avian influenza  
 Not less than 14 days and not more than 21 days after the last day of collection of eggs for 
export to Australia, a sample of the source flock was tested serologically for influenza virus 
type A with negative results.  
The sample tested was o f a sufficient size to give a 99% confidence of detecting the disease if 
there was a 5% disease prevalence in the source flock . 
Total number of birds in the source flock: ........…………...........  
Total number of birds tested: ........……………………….........…  
3.  Newcastle disease (APMV -1)   
  
Page  48 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   Not less than 14 days and not more than 21 days after the last day of collection of eggs for 
export to Australia, a sample of the source flock was tested serologically for Newcastle 
disease virus (APMV -1) with negative results . Testing was conducted on a flock sample of a 
sufficient size to give a  sample size for 99% confidence of detecting the disease if there was a 
5% disease prevalence in the source flock . 
4. Avian pneumovirus  
Vaccinated flocks only:  
 At least 14 days and no t more than 21 days after the last day of collection of eggs for export to 
Australia, the same 100 individually identified birds that were tested pre -collection were re -
tested serologically for avian pneumovirus. The individual titres are attached to the h ealth 
certificate and are considered consistent with vaccination against avian pneumovirus because:  
Either:  
o No bird showed a rise in titre of more than 2 dilutions over the first test  
or 
o One or more birds showed a rise in titre of more than 2 dilutions ov er the first. An 
explanation as to why this is considered to be consistent with vaccination against avian 
pneumovirus is attached to the health certificate along with supporting evidence  
or 
o One or more birds showed a rise in titre of more than 2 dilutions over the first and virus 
isolation tests were performed with negative results. The individual virus isolation 
results are attached to the health certificate.  
5. All tests were:  
 carried out in a government laboratory; or a laboratory approved by the governm ent of the 
exporting country for this specific purpose, and agreed to by the Department of Agriculture, 
Water and the Environment.  
 OIE-approved tests (Appendix 2), or tests agreed to by the Department of Agriculture, Water 
and the Environment.  
Test results are shown in the table below. Laboratory results and veterinary report explaining any 
positive results must be attached to the health certificate and forwarded to the Department of 
Agriculture, Water and the Environment.  
Total number of birds in the source flock: ........................................  
Disease  Laboratory  Test 
used  No of 
tests  No of positive 
results*  Details of any re -
testing  
Influenza A (Influenza virus 
type A)  
          
 
Newcastle disease (APMV -1)  
          
 
Avian pneumovirus   
 
  
 
  
 
  
 
  
  
  
Page  49 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  6. Investigations have been performed for:  
 any clinical disease or deaths above 0.5% per week in the source flock or sentinels, or  
 drop in quantity, quality, or fertility/hatchability of the eggs produced by the source flock.  
A veterinary report explaining the findings and any relevant laboratory reports are attached.  
Signature: .......................................................…….. Date: ........................................  
Government Approved Veterinarian or Official Veterinarian  
 
Name: ……………………………......................................................................................  
Address: …………………………........................... ..........................................................  
……………………………………..........................................................................................  
Email and contact number: ……………………………………..............................  
..................... .................................................................................................................  
Part B: Disease status of the country of origin. Certification by the Official Veterinarian.  
I, .............................................. .................( name ), an official veterinarian of 
....................................................... ( certifying ministry or department ) hereby certify in relation to the 
consignment of hatching eggs of poultry identified on this health certificate  that during the time from 
the last collection of eggs for export to Australia to the completion of testing for this health certificate:  
1.………………………. ( country of export ) remains officially free of notifiable highly pathogenic avian 
influenza.  
and 
Avian inf luenza has not been reported on the source flock premises or within a radius of 40 km of its 
location.  
or 
The flock(s) of origin are in a zone or compartment approved by the Department of Agriculture, Water 
and the Environment.  
[The health certificate must  indicate the option that applies]  
2. Newcastle disease (APMV -1) has not been reported on the source flock premises or within a radius 
of 40 km of its location.  
3. After due enquiry, I am satisfied that the source flock has remained closed. Any clinical ev idence of 
disease or deaths above 0.5% has been investigated and the results indicate that the following 
diseases have not occurred in the source flock:  
 Avian influenza  
 Newcastle disease (APMV -1) 
 Avian paramyxovirus type 3 (APMV -3)  
 Fowl typhoid ( Salmonella  Gallinarum)  
 Pullorum disease ( Salmonella  Pullorum)  
 Salmonella  Enteritidis infection  
 Arizona disease ( Salmonella  Arizonae)  
 Avian pneumovirus   
  
Page  50 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   Infectious bursal disease  
 Mycoplasma  iowae infection  
 Runting/stunting syndrome  
Signature: .......................................................…….. Date: ........................................  
Official Veterinarian  
 
Name: ……………………………......................................................................................  
Address: …………………………... ..................................................................................  
……………………………………..........................................................................................  
Email and contact number: ……………………………………............................ .. 
............................................................................................................................. ................  
   
  
Page  51 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Appendix 1: List: Department approved government 
accreditation schemes for Salmonella monitoring  
Commercial C hicken  
Salmonella  
Pullorum/gallinarum  Salmonella  Enteritidis  Salmonella Arizona  
 Directive 
2009/158/EC and 
Regulation 
200/2010  
 UK Poultry Health 
Scheme  
 Ontario Hatchery 
and Supply Flock 
Program  
 USDA NPIP Part 
145 Primary Meat -
Type Chicken  
 Breeding Flocks 
or Primary Egg -
Type Chicken 
Breeding Flocks: 
U.S. Pullorum -
Typhoid Clean   Directive 2009/158/EC and 
Regulation 200/2010  
 UK Poultry Health Scheme  
 Ontario Hatchery and 
Supply Flock Program  
 USDA NPIP Part 145 
Primary Meat -Type Chicken 
Breeding Flocks or Primary 
Egg-Type Chicken Breeding 
Flocks: U.S. S. Enteritidis 
Clean  
 
  Regulation 200/2010  
 UK Poultry Health Scheme  
 Ontario Hatchery and Supply 
Flock Program “Ontario 
Salmonella certified”  
 CFIA  Export of Poultry to the 
European Union – Zoonotic 
Salmonella Program  
 USDA NPIP Part 145 
Primary Meat -Type Chicken 
Breeding Flocks or Primary 
Egg-Type Chicken Breeding 
Flocks: U.S. Salmonella 
Monitored  
Turkeys  
Salmonella  
Pullorum/gallinarum  Salmonella  Enteritidis  Salmonella Arizona  
 Directive 2009/158/EC 
and Regulation 
200/2010  
 UK Poultry Health 
Scheme  
 USDA NPIP Part 145 
Turkey Breeding 
Flocks: U.S. Pullorum -
Typhoid Clean  
 
  Directive 2009/158/EC 
and Regulation 200/2010  
 UK Poultry Health 
Scheme  
 CFIA Export  of Poultry to 
the European Union – 
Zoonotic Salmonella 
Program  
 USDA NPIP Part 145 
Turkey Breeding Flocks:  
U.S. Sanitation Monitored, 
Turkeys   Regulation 200/2010  
 UK Poultry Health 
Scheme  
 CFIA Export of Poultry to 
the European Union – 
Zoonotic Salmonella 
Program  
 USDA NPIP Part 145 
Turkey Breeding Flocks: 
U.S. Sanitation 
Monitored, Turkeys  
Ducks  
Salmonella  Enteritidis  
 Directive 2009/158/EC and Regulation 200/2010   
  
Page  52 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry   UK Poultry Health Scheme  
 USDA NPIP Part 145 Meat -Type Waterfowl Breeding flocks: U.S. Salmonella Monitored  
   
  
Page  53 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Appendix 2: OIE -approved tests for the importation of hatching 
eggs of poultry (chicken and turkey)  
PATHOGEN  TESTS  
Avian influenza virus (Influenza virus type A)  ELISA  
AGID  
Newcastle disease virus (APMV -1) HIT 
Avian paramyxovirus type 3  HIT 
Infectious bursal disease virus  AGID, VN  
ELISA  
Salmonella Pullorum , Salmonella Gallinarum , Salmonella Enteritidis  RSAT  
WBTAT  
Salmonella Enteritidis  ELISA  
Avian pneumovirus  ELISA  
IFA – confirmatory only  
Mycoplasma iowae  PCR  
  
AGID     agar gel immunodiffusion test  
ELISA  enzyme -linked immunosorbent assay  
HIT haemagglutination inhibition test  
IFA immunofluorescence antibody test  
PCR      polymerase chain reaction  
RSAT  rapid serum agglutination test  
VN virus neutralisation test  
WBTAT  whole blood tube agglutination test  
   
  
Page  54 of 54 
 
 
 
26/02/2020 : Amended Health Certificates for Hatching Eggs of Poultry  Appendix 3: OIE -approved tests for the importation of hatching 
eggs of poultry (duck)  
PATHOGEN  TESTS  
Avian influenza virus (Influenza virus type A)  ELISA  
AGID  
Newcastle disease virus (APMV -1) HIT  
Infectious bursal disease virus  
  AGID, VN  
ELISA  
Salmonella Enteritidis  RSAT  
WBTAT  
ELISA  
Goose parvovirus  SN, AGID, ELISA  
Duck enteritis virus  SN  
Duck virus hepatitis type 1 (duck hepatitis A virus ) SN  
Salmonella spp.  microbiological  
 
AGID     agar gel immunodiffusion test  
ELISA  enzyme -linked immunosorbent assay  
HIT       haemagglutination inhibition test  
IFA Immunofluorescence antibody test  
RSAT    rapid serum agglutination test 
S/VN                  serum/virus neutralisation test  
WBTAT  whole blood tube agglutination test