COMMI SSION IMPLEMENTING REGUL ATION (EU) 2019/894 
of 28 May 2019 
concer ning the author isation of L-threonine produced by Escherichia coli CGMCC 7.232 as a feed 
additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Having regar d to the Treaty on the Functioning of the European Union, 
Having regar d to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 
on additives for use in animal nutr ition (1), and in particular Article 9(2) thereof, 
Whereas: 
(1)  Regulation (EC) No 1831/2003 provi des for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitte d for the author is­
ation of L-threonine produced by Escher ichia coli CGMCC 7.232 as a feed additive for use in feed for all animal 
species. That application was accompan ied by the particulars and documents required under Article 7(3) of that 
Regulation (EC). 
(3)  That application concer ns the author isation of L-thr eonine produced by Escher ichia coli CGMCC 7.232 as a feed 
additive for all animal species to be classifie d in the additive cate gory ‘nutr itional additives ’. 
(4)  The European Food Safety Author ity (‘the Auth ority’) concluded in its opinion of 2 Octobe r 2018 (2) that, under 
the proposed conditions of use, L-threonine produced by Escher ichia coli CGMCC 7.232 does not have an adverse 
effect on animal health, consumer safety or the envi ronment. It also concluded that the additive is considered as 
a poten tial skin sensitiser and an eye and skin irritant and stated a risk for the users of the additive upon 
inhalation. Theref ore, the Commission considers that appropr iate protective measures should be take n to prevent 
adverse effects on human health, in particular as regards the users of the additive. The Auth ority also concluded 
that the additive is an efficacious source of the amino-acid L-threonin e for all animal species and that in order to 
be as efficacious in ruminants as in non-r uminant species, the additive should be protect ed against degradation in 
the rumen. The Auth ority does not consider that there is a need for specific requirements of post-market 
monitoring. It also verified the repor t on the method of analysis of the feed additive in feed submitte d by the 
Reference Laboratory set up by Regulation (EC) No 1831/2003. 
(5) The assessment of L-thr eonine produced by Escher ichia coli CGMCC 7.232 shows that the conditions for author is­
ation, as provid ed for in Article 5 of Regulation (EC) No 1831/2003, are satisfied. According ly, the use of this 
additive should be author ised as specifi ed in the Annex to this Regulation. 
(6)  The measures provi ded for in this Regulation are in accordance with the opinion of the Standing Committee on 
Plants, Animals, Food and Feed, 
HAS ADOPTED THIS REGUL ATION: 
Article 1 
The substance specifi ed in the Annex, belonging to the additive categor y ‘nutr itional additives ’ and to the functional 
group ‘amino acids, their salts and analogues ’, is author ised as an additive in animal nutr ition, subject to the conditions 
laid down in that Annex. 29.5.2019 L 142/63 Official Jour nal of the European Union EN     
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Journal 2018;16(10):5458. Article 2 
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union. 
This Regulation shall be binding in its entirety and directly applicable in all Member State s. 
Done at Brussels, 28 May 2019. 
For the Commission 
The President 
Jean-Claude JUNCK ER  29.5.2019 L 142/64 Official Jour nal of the European Union EN     ANNEX 
Identifica ­
tion 
number of 
the additive Name of the 
holder of 
author isation Additive Com position, chemi cal formula, 
descr iption, analytical method. Species or 
categ ory of 
animal Maximum 
age Minimum 
content Maximum 
content 
Other provisions End of period 
of author isa­
tion mg/kg of com plete feed 
with a moisture conten t of 
12 % 
Categor y of nutr itional additiv es. Functional group: amino acids, their salts and analogues. 
3c410 — L-threonine Additive composition: 
Powder with a minimum of 98 % 
L-thr eonine (on a dry matt er ba­
sis). 
Char acter isation of the active sub­
stance: 
L-thr eonine produced by fermen ­
tation with 
Escher ichia coli CGMCC 7.232 
Chemical formula: C4H9NO3 
CAS Number: 72-19-5. 
Analytical methods (1): 
For the determi nation of L-thr eo­
nine in the feed additive: 
—  Food Chemical Codex ‘L- 
threonine monograph’ and 
—  Ion exchang e chromat ography 
coupled with post-column de­
rivatisation and optical detec ­
tion (IEC- VIS/FD) – EN ISO 
17180.  All species — — — 1.  L- threonine may be placed on 
the market and used as an addi ­
tive consisting of a preparation. 
2.  For users of the additive and 
premixtures, feed business oper ­
ators shall establish operational 
procedures and organisational 
measures to address poten tial 
risks by inhalation. Where those 
risks cannot be eliminate d or 
reduced to a minimum by such 
procedures and measures, the 
additive and premixtures shall 
be used with personal prot ective 
equipment, including breathing 
protection. 
3.  The endoto xin content of the 
additive and its dusting poten ­
tial shall ensure a maximal en­
doto xin exposure of 1 600 IU 
endoto xins/m3 air(2). 
4.  L-thr eonine may be used via 
water for drinking. 
5.  The labelling of the additive 
shall indicate the moisture con­
tent.  18 June 
2029 29.5.2019 L 142/65 Official Jour nal of the European Union EN     Identifica ­
tion 
number of 
the additive Name of the 
holder of 
author isation Additive Com position, chemi cal formula, 
descr iption, analytical method. Species or 
categ ory of 
animal Maximum 
age Minimum 
content Maximum 
content 
Other provisions End of period 
of author isa­
tion mg/kg of com plete feed 
with a moisture conten t of 
12 % 
For the determi nation of threo ­
nine in premixtures: 
—  ion exchange chromat ography 
coupled with post-column de­
rivatisation and optical detec ­
tion (IEC- VIS/FD) – EN ISO 
17180 and 
—  ion exchange chromat ography 
coupled with post-column 
derivatisation and photo ­
metr ic detection (IEC- VIS), 
Commission Regulation (EC) 
No 152/2009 (Annex III, F). 
For the determi nation of threo ­
nine in compound feed and feed 
mater ials: 
—  Ion exchang e chromat ography 
coupled with post-column 
derivatisation and photo ­
metr ic detection (IEC- VIS): 
Commission Regulation (EC) 
No 152/2009 (Annex III, F). 
For the determi nation of threo ­
nine in water: 
—  ion exchange chromat ography 
coupled with post-column de­
rivatisation and optical detec ­
tion (IEC- VIS/FD).  6.  The labelling of the additive and 
premixtures shall indicate the 
followi ng: 
‘If the additive is administe red 
via water for drinking, protein 
excess should be avoided.’ 
(1)  Details of the analytical methods are available at the following address of the Reference Laborato ry: https://ec.europa.eu/jrc/en/eurl/feed-addi tives/evaluation-repor ts 
(2)Exposure calculated based on the endoto xin level and the dusting potential of the additive according to the method used by EFSA (EFSA Journal 2018;16(10):5458); analytical method: European Phar maco ­
poeia 2.6.14. (bact erial endoto xins).   29.5.2019 L 142/66 Official Jour nal of the European Union EN