DRAFT UGANDA STANDARD  
  DUS  2397  
2021  
First  Edition  
2021-mm-dd 
 
 
 Reference number  
DUS 2397 : 2021  
© UNBS 2021   
  
Plastic b aby feeding bottle — Specification  
 DUS 2397 : 2021  
ii © UNBS 2021  – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its corr ect application  
© UNBS  2021   
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permi ssion from UNBS.  
Requests for permission to reproduce this document should be addressed to  
The Executive Director  
Uganda National Bureau of Standards  
P.O. Box 6329  
Kampala  
Uganda  
Tel: +256 41 4 333 2 50/1/2/3  
Fax: +256 41 4 286 123  
E-mail: info@unbs.go.ug  
Web: www.unbs.go.ug  DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  iii 
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ............................  iv 
1 Scop e ................................ ................................ ................................ ................................ ......................  1 
2 Normative references  ................................ ................................ ................................ ............................  1 
3 Terms and definitions  ................................ ................................ ................................ ...........................  1 
4 Requirements  ................................ ................................ ................................ ................................ .........  2 
4.1 General requirements  ................................ ................................ ................................ ...........................  2 
4.2 Specific requirements  ................................ ................................ ................................ ...........................  2 
5 Packaging  ................................ ................................ ................................ ................................ ...............  3 
6 Labelling  ................................ ................................ ................................ ................................ .................  3 
7 Sampling  ................................ ................................ ................................ ................................ ................  3 
Annex  A (normative)  Test for permanency -of pigments  ................................ ................................ ...............  4 
A1 General  ................................ ................................ ................................ ................................ ...................  4 
A2 Reagents  ................................ ................................ ................................ ................................ ................  4 
A·3 Procedure  ................................ ................................ ................................ ................................ ...............  4 
Annex  B (Normative)  Determination of Transparency  ................................ ................................ ..................  5 
Annex  C (Normative)  Drop test  ................................ ................................ ................................ ........................  6 
C·1 Sample size  ................................ ................................ ................................ ................................ ............  6 
C.2 Procedure  ................................ ................................ ................................ ................................ ...............  6 
Annex  D (informative)  Compressive deformation test  ................................ ................................ ..................  7 
D.1 Procedure  ................................ ................................ ................................ ................................ ...............  7 
Annex  E (Normative)  Sampling  ................................ ................................ ................................ ........................  9 
E.1 Scale of sampling  ................................ ................................ ................................ ................................  12 
E.1.2  Lot ................................ ................................ ................................ ................................ .........................  12 
E.1.2   Scale of Sampling  ................................ ................................ ................................ ...............................  12 
E.2 Criteria for conformity ................................ ................................ ................................ .........................  12 
E.2.4  Drop test  ................................ ................................ ................................ ................................ ...............  13 
Annex  F (Normative)  Determination of Aging resistance and ink adhesion  ................................ ...............  8 
F.1 Aging resistanc e ................................ ................................ ................................ ................................ .... 8 
F.1.1  Procedure  ................................ ................................ ................................ ................................ ...............  8 
F.2 Ink adhesion  ................................ ................................ ................................ ................................ ...........  8 
F.2.1  Procedure  ................................ ................................ ................................ ................................ ...............  8 
Bibliography  ................................ ................................ ................................ ................................ ........................  9 
 DUS 2397 : 2021  
iv © UNBS 2021  – All rights reserved  
 Foreword  
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade , Industry and 
Cooperatives established under Cap 327 , of the L aws of Uganda , as amended .  UNBS is mandated to co -
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT  Agreement  of the World Trade Organisation (WTO).  
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and  consists of key stakeholders 
including government, academia, consumer groups, private sector and other interested parties.  
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments . The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
The committee responsible for this document is Technical Committee UNBS /TC 303, [Plastics and r elated 
products ]. 
 DRAFT UGAND A STANDARD  DUS  2397  : 2021  
 
© UNBS 2021 – All rights reserved  1 
 Plastic b aby feeding bottle — Specification  
1 Scope   
This Draft Uganda standard specifies  requirements , sampling  and test methods for plastic feeding bottles 
used for nursing babies . 
This standard does no t cover teats (nipples) and glas s feeding bottles  
2 Normative references   
The following referenced documents referred to in the text in such a way that some or all of their content 
constitutes requirements of this document. For dated references, only the edition cited applies. For undate d 
references, the latest edition of the referenced document (including any amendments) applies.  
US 1675, Determination of overall migration of constituents of plastic materials and articles intended to come 
into contact with food stuffs - Methods of analys is 
3 Terms and definitions   
For the purposes of this document, the following terms and definitions apply.  
ISO and IEC maintain terminological databases for use in standardization at the following addresses:  
— ISO Online browsing platform: available at http://www.iso.org/obp  
3.1  
baby feeding  bottle or feeding bottle or nursing bottle  
bottle for feeding babies and young children  
3.2  
norminal capacity  
the volume indicated by the manufacturer that represents maximum recommended filling level  at 27 °C ± 2 °C  
3.3  
accessories  
all components  of the plastic baby feeding  bottle except the bottle teats  (nipples)  
3.4 
brimful capacity  
volume of liquid held by the baby feeding bottle when filled to the point of overflowing at 27 ± 2˚C DUS 2397 : 2021  
2 © UNBS 2021  – All rights reserved  
 4 Requirements  
4.1 General requi rements  
4.1.1 The feeding  bottle  shall be of suitable  design,  shape  and required -dimensions  
4.1.2 The shape  shall be such  that it is  easily  cleanable  and does  not permit t he food remnants to remain  
stuck inside the feeding  bottles.  
4.1.3 The body of the feeding bottle shall be smooth, both internall y and externally, free from any  defects 
like cavities, crevices, hooks, embedded  foreign matters, detrimental bubbles, streaks, flaws  and stains,  
4.1.4 The brimful capacity shall exceed the nominal capacity by a  minimum of 15 percent.  
4.1.5 The minimum scale mark an d interval marking shall  not be more than 20 percent of the maximum 
scale indicating mark.  
4.1.6 The scale marks and the indicating numerical values shall be clear and shall not be affected by high 
temperature sterilizing treatment  
4.1.7 The feeding bottle and its parts shall not have sharp edges and sharp points that could cause injury 
to the user  
4.1.8  If the feeding bottle is unprinted, the capacity scale shall be engraved on the bottle and if the bo ttle is 
printed, the capacity scale shall be clearly printed  
4.2 Specific requirements  
4.2.1  The plastic baby feeding  bottle shall conform to the specific requirements in Table 1  when tested in 
accordance with the test methods prescribed therein  
Table 2 ─ Specific  requirements  for baby feeding bottles  
S/N Characteristic  Requirement  Test method  
i Migration of plastic constituents  No migration  US 1675  
ii Transparency  (min), % Polycarbonate bottle  85 Annex B  
Polypropylene  bottle  60 
iii Permanency of pi gment  Printed impressions do not become illegible  Annex A  
iv Rapture and leakage  No sign of rupture or leakage  Annex C  
v Pressurized deformation resistance , 
max, % 10 Annex D  
vi Aging resistance/ ink adhesion  No significant removal of the print from the  surface 
of the container and the print shall be legible to 
the naked eye  Annex E 
vii Wall thickness, mm  Within ±10 of declared value  Annex F 
viii Bisphenol -A, min, mg/kg  0.05 Annex G 
 DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  3 
  
4.2.2 The capacity of feeding bottles shall be as indicated in tab le 2. 
Table 2  ─ Capacity of baby feeding bottles (Maximum indication scale)  
Indicated Capacity in ml  40-200 210-290 300-450 
Tolerance on Maximum indication scale  ±4 ±5 ±6 
5 Packaging  
The feeding bottle shall be pack aged in suitable containers that guarantee product in tegrity during 
transportation, storage and handling.  
6 Labelling  
6.1 Each feeding bottle shall be permanently marked with scale mark.  
6.2 Each package  containing the bottles shall be permanently marked with the following:  
a) name and physical address of the m anufacturer  
b) name of product as, “baby feeding bottle ” or “baby bottle” or “nursing bottle ” 
c) nominal capacity;  
d) batch number /code number  
e) date of manufacture (month and year)  
f) material used  (resin code)  
g) country of origin  
h) cleaning and sterilization instructions   
i) instructions for disposal and recycle  
j) shelf life  
7 Sampling  
The sampl ing shall be done in accordance with  Annex  H DUS 2397 : 2021  
4 © UNBS 2021  – All rights reserved  
 Annex  A 
(normative)  
 
Test for permanency -of pigment s 
A1 General  
This test is meant only for those feeding bottles which have a printed scale  and graduations.  
A2 Reagents  
A.2.1  Sodium Dichromate  
A·2.2  Concentrated Sulphuric Acid, Relative density, 1.834  
A·3 Procedure  
A.3.1  Weight about 20 g of sodium dichromate  and dissolve in 1500 ml of  concentrated  sulphuric acid and 
dilute to 2 500 ml with wa ter. Immerse  the bottles in the solution at room temperature for 15 min. Rinse the 
samples with water and dry.  
A.3.2 The bottles shall be taken as having satisfied the requirements of the test if the printed impressions do 
not become illegible.  DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  5 
 Annex  B 
(Normative ) 
 
Determination of Transparency  
B.1 Test specimen shall be prepared from the part of feeding bottle where scale marks or other marks are 
not found.  
B.2 Apparatus ─ Light transmittance measurement device  
B.3 Test specimen ─ The size of test specimen shall be 50 × 50 mm and the thickness shall be the original 
thickness of the test specimen. Three test specimens shall be prepared.  
B.4 Measurement ─ Install the white  standard plate, adjust the reading (T 1) of the device’s current meter to 
be 100; adjust the amount of incident light. Under the status where the white standard plate is installed, install 
and measure the test specimen to obtain the indication (T 2) of the current meter. The full light transmittance 
shall be calculated according to the following formula:  
 
 
Where  
T full light transmittance (percent).  
 DUS 2397 : 2021  
6 © UNBS 2021  – All rights reserved  
 Annex  C 
(Normative ) 
 
Drop test  
C·1 Sample size  
The sample  size shall be ten bottles, taken at random from a batch, divided into two sets of 5 each, designate 
as Set 1 and Set 2.  
C.2 Procedure  
C.2.1 Fill each bottle with water at ambient conditions and close tight with closures.  
C.2.2 Drop the bottles under free fall conditions in Set 1 squarely on their base on to a rigid flat horizontal 
surface of steel or smooth concrete as the dropping surface.  
C.2.3 Drop the bottles under free fall condition in Set 2 on their side (the body of the bottle being parallel to 
the impacting floor) onto the dropping surface.  
C.2.4 Examine each bottle for signs of rupture or leakage.  DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  7 
 Annex  D 
(informative)  
 
Compressive deformati on test  
D.1 Procedure  
Apply the compressive load of 2 kg in the middle part of the body or to the part having the maximum diameter  
of a feeding bottle by the use  of compression . Measure the deflection of the part at that time, and calculate 
percentage deflection. The m easurements shall be carried out  at 27 ± 2°C  
 
Where  
D Percentage of deflection of diameter  
D1 Outside  diameter  prior to test  
D2 Outside  diameter  at the time of compression  DUS 2397 : 2021  
8 © UNBS 2021  – All rights reserved  
 Annex  E 
(Normative)  
 
Determination of Aging resistance and ink adhesion  
E.1 Aging resistance  
E.1.1 Procedure  
E.1.1.1 Immerse the bottles into the boiling water for 20 min, then immediately into the ice water for 20 
min alternately and repeat it 3 times. At the end of the test, the change in the capacity of bottles shall not be 
more t han 1 percent and also there shall be no defective changes in the bottle.  
E1.1.2 There shall be no significant changes in appearance when the accessories are tested in 
accordance with the method  in Annex F  
E.2 Ink adhesion  
E.2.1 Procedure  
E.2.1.1 Apply tw o strips of 25 mm wide transparent pressure sensitive tape or cello tape to the printed 
area of container; one piece down the length of the container and the other round the circumference.  
E.2.1.2 Press the tape firmly on to the container and leave it for 15seconds.  
E.2.1.3 Remove the tape by pulling slowly at about 1 cm/s from one end at about 90° to the container 
surface.  
E.2.1.4 There shall be no significant removal of the print from the surface of the container and the print 
shall be legible to the nake d eye after the test  
 DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  9 
 Annex  F 
(normative)  
 
Determination of wall thickness  
F.1 Apparatus  
Micrometer/screw gauge, fitted with ball point tips or dial caliper gauge fitted with spherical anvils giving an 
accuracy of measurement of 0.02 mm.  
F.2 Procedure  
The container wall thickness shall be ascertained by either of  the methods indicated below  
F.2.1  Micrometer metho d 
Cut the plastic feeding bottle  horizontally into three pieces (top, m iddle and bottom) Measure the wall 
thickness with a micrometer or screw gaug e fitted with ballpoint tip, at four places in each section. Take the 
average of four readings and report as wall thickness at top, middle and bottom.  
.F.2.2  Dial caliper gauge method  
Measure  the wall thickness with the help of  dial caliper fitted with sph erical  anvils. Care shall be taken to avoid 
movement  of the container  during measurement  as this may affect the reading obtained. The measurement 
shall be to an accuracy of  0.02 mm. Take the mean of three readings at any location  (top, middle and bottom) 
as wall thickness.  DUS 2397 : 2021  
10 © UNBS 2021  – All rights reserved  
 Annex  G 
(normative)  
 
Determination of Bisphenol A  
G.1 Introduction  
Plastic food packaging or cont ainers are produced with the help of plasticizers or additives used in plastic 
manufacture to improve its ﬂexibility, stability, or resistance properties. Among these additives, 2,2 -bis(4 -
hydroxyphenyl) -propane (Bisphenol -A) is a monomer used for the synth esis of polycarbonate (PC) . Bisphenol 
A (BPA) is a monomer used to make polycarbonate plastic and epoxy resins. Traces of BPA can leach out of 
these polycarbonate plastic surfaces under various environmental conditions such as heat or pH changes and 
eventu ally are consumed by humans.  
G.2 Reagents and materials  
G.2.1  Purified water  
G.2.2  Acetonitrile (HPLC grade ) 
G.2.3  Methanol (HPLC grade ) 
G.2.4  Potassium phosphate monobasic  
G.2.5  Standards of bisphenol A  
G.3 Chromatographic parameters  
The chromatograph ic parameters for reverse phase liquid chromatography.  
G.3.1  Column :       C18 4.6×100 mm 5 µm or 4.6×100 mm 1.8 µm  
G.3.2  TCC temperature:     40 °C  
G.3.3  FLD:        Ex: 230. Em: 316  
G.3.4  FLD acquisition rate, gain:   9.26 Hz, 15  
G.3.5  Sample thermostat:    4 °C 
G.3.6  Mobile phase A:     10 mM monobasic potassium phosphate in water  
G.3.7  Mobile phase B:     100% Acetonitrile  
G.3.8  Flow:       0.9 mL/min  
G.3.9  Injection volume:     20 µL. 5 sec needle wash at flush port for 5 sec, using mobile phase 
A. DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  11 
 G.3.1 0 Gradient: Time  
Time (min)  %B 
0 5 
2 5 
2.1 35 
12.5 35 
12.6 70 
17 70 
18.1 5 
23 5 
G.4 Standard preparation  
G.4.1  Preparation of stock solution  
Weigh accurately the BPA standard, dissolve in 100% methanol to obtain stock solutions of about 300 µg/mL  
store at 4 °C when not in use.  
G.4.2  Preparation of standards BPA  
Prepare freshly a 400 ng/mL solution of BPA by diluting the stock solutions using the dilution buffer of 5% 
acetonitrile and 95% 10 mM monobasic potassium phosphate in water  
G.5 Sample pr eparation  
Add 250 mL boiling water into baby bottle . Place baby bottle in boiling water for 30 minutes . Transfer the 
water from the baby bottle into amber colored bottles . Acidify using concentrated HCl to pH 2.0 and store at 4 
°C. Precondition SPE using 6  mL methanol and 6 mL water . Add 50 ml acidified water , elute using 2 × 4 mL of 
100% methanol . Evaporate under a stream of nitrogen at 60 °C and reconstitute in 1 mL of dilution buffer . 
G.6 Procedure  
Inject blank. Inject standard preparation in five replic ate and record the chromatogram. It should comply with 
system suitability.  
Inject sample preparation in duplicate, record the chromatogram and calculate the percent content of BPA.  DUS 2397 : 2021  
12 © UNBS 2021  – All rights reserved  
 Annex  H 
(Normative)  
 
Sam pling  
H.1 Scale of sampling  
H.1.2 Lot 
In any consignment, all the bottles of the same material, size and drawn from a single batch of manufacture 
shall be grouped together to constitute a lot  
H.1.2  Scale of Sampling  
For ascertaining the conformity of the lot to the requirements of this standard, tests shall be carried out for 
each lot separately. The number of bottles to be sampled from a lot sha ll be in accordance with Table H 1. 
Table H 1 ─ Scale of sampling  and acceptance n umber  
SN Lot Size  Manufacture, w orkmanship, 
finish and appearance  For transparency, leakage, ageing resistance, compress ive 
deformation resistance, Bisphenol A and I nk Adhesion for 
Printed Bottles  
Sample size  Acceptance 
number  Sample size  Acceptance number  
1 Up to 500  13 1 5 0 
2 501 to 1000 20 2 8 0 
3 1001 to 3 000 32 3 13 0 
4 3001 to 5000  50 5 20 1 
5 5001 and above  80 7 32 2 
 
H.2.3 The bottles shall be selected at random from the lot  
H.2 Criteria for conformity  
H.2.1 Manufacture, Workmanship, Finish and Appearance  
The sample bott les selected as per col 2 of  Table H 1 shall be examined for requirements in clause 4.1. Any 
bottle failing in one or more of the requirements shall be termed as defective. The lot shall be accepted under 
this head if the number of defective bottles in samp le does not exceed the acceptance number given in col 3 
of Table H 1. 
H.2.2 Capacity and wall thickness dimensions  
Five bottles for a lot size up to 5000 and 10 bottles for lot size above 5000 shall be selected at random from 
the samp les already drawn acco rding to H .2.3. There shall be no failure if the lot is to be accepted under this 
clause.  DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  13 
 H.2.3 Permanency  of pigments, Transparency, Leakage test, Ageing resistance, c ompr essive deformation 
resistance and ink adhesion test. The number of sample bottles to  be drawn shall be in accordance  to col 4 of 
Table H 1. Each of the samp le bottle shall be subjected to the respective tests. The number of failures shall not 
exceed the acceptance number given in col 5 of Table H 1 for all tests except leakage test. For lea kage and 
Bisphenol -A test the acceptance  number  is zero, that is no failure shall occur for lot acceptance  
H.2.4 Drop test  
The sample  bottles shall be drawn from the lot and these shall be subjected to drop test. There shall be no 
rupture or leakage in any  bottle after the test for acceptance. In case even one bottle has any sign of rupture 
or leakage, the lot shall be considered as not conforming to the requirements of  this specification  DUS 2397 : 2021  
14 © UNBS 2021  – All rights reserved  
 Bibliography  
[1] DKS 2607: 2015 , Baby feeding bottles — Specification  
[2] IS 14625: 1999, Plastics feeding bottles  
[3] Journal of Analytical Methods in Chemistry Volume 2019, Article ID 1989042 ─ Validation of an HPLC 
Method  for Determination  of Bisphenol -A Migration from Baby Feeding Bottles  DUS  2397 : 2021  
© UNBS 2021  – All rights reserved  15 
  
 DUS  2393: 2021  
© UNBS 2021 – All rights reserved  17 
 Certification marking  
Produc ts that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the figure below.  
The use of the UNBS Certification Mark is governed by the Standards Act, and the Regulations made 
thereunder. T his mark can be used only by those licensed under the certification mark scheme operated by 
the Uganda National Bureau of Standards and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing in accordance with the 
certification mark scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually c hecked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
 DUS 2393: 2021    
 
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