[Attachment]  
 
 
 
 
 
 
 
Survey guidelines  for major fruit flies  
 
 
 
 
 
 
 
 
 
 
 A. Terms  
1) Detection and outbreak  
Detection  • pre-outbreak phase  
• considered as pest free but emergency measure is implemented  
• Target fruit fly  has not been established but an isolated group 
capable of survival for forseeable future is detected  
Outbreak  • notification to international organizations and trade partners etc.  
• domestic and international pest reporting  
• official control measures are  implemented  
• Significant number of target fruit fly  has been established  
 
 
2) Criteria of fruit fly outbreak  
Fruit Fly 
species  Criteria  
Bactrocera 
dorsalis complex  • more than 1 larva, pupa or mated female or  
• more than 5 adults of one generation within 1.6km × 1.6km 
are found in the traps(male, unmated female)  
Ceratitis 
capitata  • more than 1 larva, pupa or mated female or  
• more than 2 adults of one generation within 4.8km radius are 
found in the traps  
B. cucurbitae, B. 
tryoni  • if 2 flies/km/2weeks detected: monitoring conducted twice a 
week + additional traps + larva survey → if additional 3 flies 
detected/2weeks → outbreak  
• more than 5 flies/km/2weeks or mated female or larva 
detected  
* When other type of fruit fly is detected, similar fruit fly criteria is applied  
 
B. Survey and Control Measures  
1) Method: Trap survey and field survey  
a) Period: May -October (trap inspection conducted every two weeks)  
(April - November in Jeju  area (trap inspection conducted every two weeks))  
b) Subjects : Host fruits  
c) Areas: Nationwide  
d) Method  
(1) Survey method  APQA: Conducts regular survey in airports, harbors and exporting area where the 
traps are installed in conjunction with survey on decomposed fruits . 
(2) Trap survey  
(a) Attractant for fruit fly  
Target fruit fly  Attractant  
Ceratitis capitata  
Bactrocera dorsalis  
B. cucurbitae, Bactrocera tryoni  
Other fruit flies and females  Capilure  
Dorsalure  
Cuelure  
Proteinlure  
 
(b) Record keeping of trap installation : The designated number of traps are 
recorded and the location is marked on a map.  
 
2) Post -detection measures  
A) Emergency measures to prevent the spread  
(1) Spraying pesticides  
(a) Attractant and pesticide placement  
Thirty lures with the attractant mixed with 1% of pes ticide such as Fenitrothion 
and absorbed it in porous fiber board or cotton strings are installed within 256ha 
(1.6km×1.6km) in vicinity of detection location.  
* Types of attractant may be modi fied according to situation and more traps and Protein 
hydrate  may be used.  
(b) Soil treatment  
Pesticide such as Diazinon  (granule) is drenched (sprayed) under all host fruit 
trees located within 200m of detection location of adult fruit flies at intervals of 
15 days in order to destroy pupa in soil.  
* Methods and rates of application  may be adjusted depending on the types o f pesticide.  
(c) Spraying pesticides  
Pesticide is sprayed within 200m of detection area in accordance with 
Agrochemicals Use Guide Book published by Korea Crop Production 
Association (KCPA).  B) Delimiting survey  
(1) Trap deployment  : The traps are not installed in areas such as rice paddy, forests 
and non -host farmlands etc., and traps are not installed even when additional flies 
are detected within the already installed square grids area.  
(a) Ceratitis capitata  
Square grids are esta blished at intervals of 1.6km around initial detection location, 
place 100 -50-25-20-10 traps per 256ha(1.6km x 1.6km) from the core to edge. 
Additional 25 Macphail traps are added at the core area,  
 
Survey trap deployment  
 
          Core area,   1st buffer    2nd buffer   3rd buffer    4th buffer area  
           area          area         area 
* 25 Mcphail traps are installed at the core area  
 
(b) Bactrocera dorsalis complex  
Square grids are established at intervals of 1.6km around initial detection location 
and 25 -5-5-5-5 traps per 256ha from core to edge are deployed. Additional 25 
Mcphail traps are installed at the core.  
(c) Bactrocera cucurbitae. Bactrocera tryoni  
Square g rids at intervals of 1.6km around initial detection location are established 
and 50 -25-15-10-5 traps per 256ha from core to edge are deployed. Additional 25 
Mcphail traps are installed at the core.  
(d) Other types of fruit flies  
Square grids at intervals o f 1.6km around initial detection location are established, 
80-40-20-10-5 traps per 256ha from  core to edge are deployed.  
(2) Survey methods  
(a) Period and frequency of trap survey  
① Period: Until the third generation since last detection (two generations in case 
of single detection)  
② Frequency: Daily survey for a week of first generation period is conducted. 
Then two surveys a week are conducted. For second and third 
generation, one survey is conducted for every two weeks.  
(b) In conjunction with trap inspection, fruits are sampled to check for larva 
infection. In particular, a roster of orchards located within 200m of detection 
location is developed and host plants grown in fields and gardens are surveyed 
(fruits located wi thin 2.4km of detection location at intervals of 7~10days).  
(c) Female fruit flies caught in traps are inspected to find out whether it has been 
mated,  
(d) Along with trapping and fruit sampling, interview of relevant people is 
conducted to figure out path way of introduction.  
 
C. Eradication  
1) Criteria for conducting eradication  
a) Onset of eradication  
Eradication measure is implemented once fruit fly outbreaks. However  for fruit 
fly detection status, only monitoring activities (trapping and fruit sampling) for  two 
generation period is enhanced.  
b) Fruit collection and stripping  
All fruits within 200m of outbroken location are collected and incinerated or 
buried in more than 50cm depth.  
* When burying, quicklime (0.4%) is added.  
c) Placement and spraying of attractant and pesticide  (1) For Bactrocera dorsalis,  B. cucurbitae, Bactrocera tryoni: One % of pesticide 
such as Fenitrothion  and so on are  added to Methyl eugenol(Dorsalure) or Ceulure  and 
absorbed in porous fiber plates or cotton strings. Thirty plates or strings with pesticide and 
attractant are placed within 256ha (1.6km×1.6km)  of detected location. This is conducted for 
three generations from last detection.  
(2) For Ceratitis capitat a and other fruit flies:  One % of pesticide such as Fenitrothion 
and so on are added to Capilure or relevant lure and sprayed twice a week for the first 6 
weeks in areas located at least 2.4km from initial detection location. It may be 
sprayed once a week if no longer detected.  
- Spraying may be stopped when flies are no longer detected for  two generation 
period from the last de tection .  
(3) If outbreak is severe,  protein hydrate is added to attractant for spraying.  
* Only protein hydrate may be added instead of pheromone lure depending on situation  
d) Soil treatment  
For host plants located 200m within detected location, pesticide is 
drenched(sprayed) under host fruit trees at intervals of 14~16 days.  
* Cycle and frequency may be adjusted according to type of pesticide  
 
D. Regulatory Measures on Movement and Cropping etc.  
1) Procedures for emergency measures  
Regulatory measures follows below in order until fruit flies are eradicated.  
a) Relevant growers, agents, and processors located within 7.2km radius of the 
detection location are notified with emergency measures.  
b) Appropriated quarantine measures (plans) are establ ished after reviewing 
ecosystem, climate conditions and spread status of fruit flies.  
2) Items need to be included in regulatory measures  
The extent of regulatory activity required is dependent on the degree of infestation.  
(For minor and limited outbreak,  sales of fruits in small amount may not be 
regulated.)  
a) Monitoring of phytosanitary treatment status in fruits in commercial area and issuance of certificates  
b) Regular supervising  to agricultural products processing facility  
c) Monitoring of regulated  plant wastes  
d) Check of passenger baggages at airports and the judicious use of road patrols and 
roadblocks may be necessary where heavy infestation occurs.  
e) Monitoring of movements in highways and local roads etc. which are adjacent 
quarantine boundar ies. 
※ In case of Ceratitis capitata , if outbreak are a is confined to one and is not  
serious, spraying, trap deployment and quarantine measures may be conducted 
within 1km.  
 
E. Confirming Effectiveness of Eradication and Declaration  
1) Trap inspection  
a) Trap inspection is conducted for three generations from the last detection  
2) Fruit sampling  
a) Fruits from the  core and 1st buffer area(2.4km from detection location) are 
sampled at intervals of 7~10 days. In particular, roster of host plants at the cent er 
should be developed and fruit cutting inspection is conducted.  
b) For areas equipped with facilities, collected fruits are examined after being 
incubated for 1 week ~ one generation period under 26 ℃/70% of relative  
humidity which is an ideal condition f or fruit flies to grow. Facilities where fruits 
are kept should check for safety in order to prevent adult fruit flies from escaping.  
3) Others  
Survey is conducted for  three generation period by setting up 5 traps at intervals of 
1.6km in areas besides in tensive survey area.  
4) Declaration of eradication  
If fruit flies are not detected after the measures for three generations from the last 
detection, it is considered to be eradicated.