COMMI SSION IMPLEMENTING REGUL ATION (EU) 2018/1543 
of 15 Oct ober 2018 
concer ning the author isation of a preparation of Pediococcus pentosaceus DSM 32291 as a feed 
additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Having regar d to the Treaty on the Functioning of the European Union, 
Having regar d to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 
on additives for use in animal nutr ition (1), and in particular Article 9(2) thereof, 
Whereas: 
(1)  Regulation (EC) No 1831/2003 provi des for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitt ed for the author isa­
tion of a preparation of Pediococcus pentosaceus DSM 32291. That application was accompanied by the particulars 
and documents required under Article 7(3) of Regulation (EC) No 1831/2003. 
(3)  That application concer ns the author isation of a preparation of Pediococcus pentosaceus DSM 32291 as a feed 
additive for all animal species, to be classified in the additive categor y ‘technological additives ’. 
(4)  The European Food Safety Authority (‘the Author ity’) concluded in its opinion of 21 Febr uary 2018 (2) that, 
under the proposed conditions of use, the preparation of Pediococcus pentosaceus DSM 32291 does not have an 
adverse effect on animal health, human health or the environment. The Author ity also concluded that the 
preparation concer ned has the poten tial to impro ve the production of silage from easy , and moderately difficult 
to ensile forag e mater ials. The Author ity does not consider that there is a need for specific requirements of 
post-market monitori ng. It also verified the repor t on the method of analysis of the feed additive in feed 
submitte d by the Reference Laborat ory set up by Regulation (EC) No 1831/2003. 
(5) The assessment of the preparation of Pediococcus pentosaceus DSM 32291 shows that the conditions for author isa­
tion, as provid ed for in Article 5 of Regulation (EC) No 1831/2003, are satisfied. According ly, the use of that 
preparation should be author ised as specif ied in the Annex to this Regulation. 
(6)  The measures provi ded for in this Regulation are in accordance with the opinion of the Standing Committee on 
Plants, Animals, Food and Feed, 
HAS ADOPTED THIS REGUL ATION: 
Article 1 
The preparation specifi ed in the Annex, belonging to the additive catego ry ‘technological additives ’ and to the functional 
group ‘silag e additives’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that 
Annex. 
Article 2 
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union. 16.10.2018 L 259/22 Official Jour nal of the European Union EN     
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Journal 2018; 16(3):5202. This Regulation shall be binding in its entirety and directly applicable in all Member State s. 
Done at Brussels, 15 October 2018. 
For the Commission 
The President 
Jean-Claude JUNCK ER  16.10.2018 L 259/23 Official Jour nal of the European Union EN     ANNEX 
Identifica ­
tion 
number of 
the additive Additive Com position, chemi cal formula, 
descr iption, analytical method Species or 
category of 
animal Maximum 
age Minimum 
content Maximum 
conten t 
Other provisions End of 
period of 
author isation CFU of additive/kg of fresh 
material 
Technological additiv es: silage additiv es 
1k21015 Pediococcus 
pentosaceus 
DSM 32291 Additive composition 
Preparation of Pediococcus pento ­
saceus DSM 32291 containing 
a minimum of 8 × 1010 CFU/g 
additive. 
Char acter isation of the active sub­
stance 
Viable cells Pediococcus pentosaceus 
DSM 32291 
Analytical method (1) 
Enumeration in the feed additive: 
spread plate method on MRS agar 
(EN 15786) 
Identif ication of the feed additive: 
Pulsed Field Gel Electrophoresis 
(PFGE). All animal 
species — — — 1.  In the directions for use of the additive 
and premixture, the storage conditions 
shall be indicated . 
2.Minimum content of Pediococcus pentosa ­
ceus DSM 32291 when used without 
combination with other micro-org anisms 
as silage additives: 5 × 107 CFU/kg of 
easy and moderately difficult to ensile 
fresh mate rial(2). 
3.For users of the additive and premix ­
tures, feed business operators shall estab ­
lish operational procedures and organisa ­
tional measures to address pote ntial risks 
resulting from its use. Where those risks 
cannot be eliminate d or reduced to 
a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing prot ec­
tion. 5.11.2028 
(1)  Details of the analytical methods are available at the following address of the Reference Laborato ry: https://ec.europa.eu/jrc/en/eurl/feed-addi tives/evaluation-repor ts 
(2)  Easy to ensile forag e: > 3 % soluble carbohy drates in fresh mat erial. Moderatel y difficult to ensile forag e: 1,5 %-3,0 % soluble carboh ydrates in the fresh mat erial. Commission Regulation (EC) No 429/2008 of 
25 Apr il 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regar ds the preparation and the presentation of application sand 
the assessment and the author isation of feed additives (OJ L 133, 22.5.2008, p. 1).   16.10.2018 L 259/24 Official Jour nal of the European Union EN