FOR COMMENTS ONLY FINAL DRAFT UGANDA 
STANDARD  
  FDUS EAS 383 
First  Edition  
2013-mm-dd 
 
 Reference number  
FDUS EAS 383: 2013 
© UNBS 201 3 
  
Synthetic organic liquid detergent for household use — 
Specification  
 FOR COMMENTS ONLYFDUS EAS 3 83: 2013 
ii                © UNBS  2013 - All rights reserved 
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2013 
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS. 
Requests for permission to reproduce this document should be addressed to 
The Executive Director 
Uganda National Bureau of Standards 
P.O. Box 6329 
Kampala 
Uganda 
Tel: 256 41 505 995 
Fax: 256 41 286 123 
E-mail: unbs@infocom.co.ug  
Web: www.unbs.go.ug  
 FOR COMMENTS ONLYFDUS EAS 3 83: 2013 
© UNBS  2013 - All rights reserved                iii National foreword 
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Tourism, Trade and 
Industry established under Cap 3 27, of the Laws of Uganda. UNBS is mandated to co-ordinate the elaboration 
of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT/SPS Agreements of the World Trade Organisation (WTO). 
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of representatives of 
consumers, traders, academicians, manufacturers, government and other stakeholders. 
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council. 
This Final Draft Uganda Standard, FDUS EAS  383: 2013, Synthetic organic liquid detergent for household use 
— Specification , is identical with  and has been reproduced from an East African  Standard, EAS 383: 2013 , 
Synthetic organic liquid detergent for household use  — Specification , and is being proposed for adoption as a 
Uganda Standard.  
This standard was developed by the Chemicals and environment Standards Technical Committee (UNBS/TC 
5). 
Wherever the words, “ East African Standard" appear, they should be replaced by "Uganda Standard." 
 FOR COMMENTS ONLY EAS 383: 2013 
ICS 71.100.40 
© EAC 2013                                                                                                                       Second Edition 2013  
 
EAST AFRICAN STANDARD 
Synthetic organic liquid detergent for household use — Specification  
EAST AFRICAN COMMUNITY FOR COMMENTS ONLYFOR COMMENTS ONLYEAS 383: 2013 
ii © EAC  2013  – All rights reserved  
 Copyright notice 
This EAC document is copyright-protected by EAC. While the reproduction of this document by 
participants in the EAC standards development process is permitted without prior permission from EAC, 
neither this document nor any extract from it may be reproduced, stored or transmitted in any form for 
any other purpose without prior written permission from EAC. 
Requests for permission to reproduce this document for the purpose of selling it should be addressed 
as shown below or to EAC’s member body in the country of the requester:  
© East African Community 2013  — All rights reserved 
East African Community  
P.O.Box 1096 
Arusha 
Tanzania 
Tel: 255 27 2504253/8 
Fax: 255 27 2504481/2504255 
E-mail: eac@eachq.org 
       Web: www.eac-quality.net 
Reproduction for sales purposes may be subject to royalty payments or a licensing agreement. 
Violators may be persecuted FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  iii 
 Contents Page 
Foreword ............................................................................................................................................................  iv 
1 Scope  ................................ ................................ ................................ ................................ ...................... 1 
2 Normative references  ................................ ................................ ............................................................  1 
3 Terms and definitions  ................................ ................................ ...........................................................  1 
4 Requirements  ................................................................ ................................ ................................ ......... 2 
4.1 General requirements  ................................ ................................ ...........................................................  2 
4.2 Specific quality requirements  ................................ ................................ ................................ .............. 3 
5 Storage  ................................ ................................ ................................ ................................ ................... 3 
6 Packaging and marking  ................................ ................................ ................................ ........................ 3 
6.1 Packaging  ................................ ................................ ................................ ................................ ............... 3 
6.2 Marking  ................................ ................................ ................................ ................................ ................... 3 
7  Sampling  ................................ ................................ ................................ ................................ ............... 4 
8 Criteria for conformity ................................ ................................ ...........................................................  4 
Annex A (normative)  Test for solubility  ................................ ................................ ................................ .......... 5 
A.1 Preparation of synthetic hard water  ................................................................ ................................ .... 5 
A.2 Procedure  ................................ ................................ ................................ ................................ ............... 5 
Annex B (normative)  Test for rinsing properties  ................................ ...........................................................  6 
Annex C (normative)  Methods of test for synthetic detergents  ................................ ................................ ... 7 
C.1 Quality of reagents  ................................ ................................ ................................................................  7 
C.2 Qualitative identification of non-soapy detergents  ................................ ................................ ........... 7 
C.2.1  General  ................................ ................................ ................................ ................................ ................... 7 
C.2.2  Apparatus  ................................ ................................ ................................ ................................ ............... 7 
C.2.3  Reagents  ................................ ................................ ................................ ................................ ................ 7 
C.2.4  Procedure for identification of soapy and non-soapy detergents  ................................ ................... 7 
C.2.5  Procedure for identification of the type non-soapy detergents  ................................ ....................... 7 
C.3 Quantitative identification of active ingredient  ................................ ................................ .................. 8 
Annex D (normative)  Determination of pH  ................................................................ ................................ ...... 9 
D.1 General  ................................ ................................ ................................ ................................ ................... 9 
D.2 Apparatus  ................................ ................................ ................................ ................................ ............... 9 
D.3 Reagents  ................................ ................................ ................................ ................................ ................ 9 
D.4 Procedure  ................................ ................................ ................................ ................................ ............... 9 
Annex E (normative)  Determination of non-detergent organic matter  ................................ ...................... 10 
E.1 General  ................................ ................................ ................................ ................................ ................. 10 
E.2 Apparatus  ................................ ................................ ................................ ................................ ............. 10 
E.3 Reagents  ................................ ................................ ................................ ................................ .............. 10 
E.4 Procedure  ................................ ................................ ................................ ................................ ............. 10 
E.5 Calculation  ................................................................ ................................ ................................ ........... 11 
Annex F (normative)  Sampling ...................................................................................................................... 12 
F.1 Procedure  ................................ ................................ ................................ ................................ ............. 12 
F.2 Preparation of test samples  ................................ ................................ ................................ ............... 12 
F.2.1  Composite sampl e ................................ ................................................................ ...............................  12 
F.2.2  Samples for testing  ................................ ................................ .............................................................  12 
 FOR COMMENTS ONLYEAS 383: 2013 
iv © EAC  2013  – All rights reserved  
 Foreword 
Development of the East African Standards has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that throu gh 
harmonized standardization, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
In order to achieve this objective, the Community established an East African Standards Committee mandated 
to de velop and issue East African Standards.   
The Committee is composed of representatives of the National Standards Bodies in Partner States, together 
with the representatives from the private sectors and consumer organizations. Draft East African Standards 
are circulated to stakeholders through the National Standards Bodies in the Partner States. The comments 
received are discussed and incorporated before finalization of standards, in accordance with the procedures 
of the Community. 
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards are therefore expected to ensure that they always have the latest versions of the standards 
they are implementing .  
EAS 383 was prepared by Technical Committee EAS/TC 0 74, Surface active agents . 
This second edition cancels and replaces the first edition (EAS 383:2005), which has been technically revised. FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  v 
 Introduction 
This specification refers to non-soapy organic detergents and in particular anionic detergents. These may 
contain non-ionic active ingredients. Inorganic compounds such as phosphates and silicates generally termed 
as builders may also be included in this products. 
Branched alky aryl sulphonates are unbiodegradable. The use of these starting material s is strongly 
discouraged for environmental conservation. However when these starting materials are used they shall be 
declared by the manufacturer.  FOR COMMENTS ONLYFOR COMMENTS ONLYEAST AFRICAN STANDARD  EAS 383:2013  
 
© EAC 2013  – All rights reserved  1 
 Synthetic organic liquid detergent for household use — Specification  
1 Scope 
This East African Standard prescribes the requirements and the method of sampling and test for 
synthetic liquid detergents for household use both for general purpose and dishwashing liquid 
detergent. 
2 Normative references 
The following referenced documents are indispensable for the application of this document. For dated 
references, only the edition cited applies. For undated references, the latest edition of the referenced 
document (including any amendments) applies. 
EAS 377-1 , Cosmetics and cosmetics products — Part 1: List of substances prohibited in cosmetic products 
EAS 377-2, Cosmetics and cosmetics products — Part 2: List of substances which cosmetic products must 
not contain except subject to restrictions laid down  
EAS 377-3 , Cosmetics and cosmetics products — Part 3: List of colourants allowed in cosmetic products 
EAS 377-4 , Cosmetics and cosmetics products — Part 4: List of preservatives allowed in cosmetic products 
EAS 377-5 , Cosmetics and cosmetics products — Part 5: Use of UV filters in cosmetic products  
ISO 2271; Surface-active agents — Determination of anionic active matter by manual or mechanical direct two 
phase titration procedure. 
3 Terms and definitions 
For the purposes of this standard, the following terms and definitions shall apply. 
3.1 
active matter 
in the form ulation, the whole of surface active agents responsible for an activity specified 
3.2 
anionic surface active 
surface active agents which ionizes in aqueous solution to produce negatively charged organic ions which are 
responsible for surface active 
3.3 
build er 
complementary component of a detergent usually inorganic, which with reference to the washing action adds 
its characteristic to those of constituents FOR COMMENTS ONLYEAS 383: 2013 
2 © EAC  2013  – All rights reserved  
 3.4 
cationic surface active agent 
surface active agent which ionises in aqueous solution to produce positively charged organic ions which are 
responsible for surface activity 
3.5 
detergent 
product specially formulated for cleaning through the process of detergency 
3.6 
foam 
mass of gasses cells separated by thin films of liquid and formed by juxtaposition of bubbles, giving a 
dispersion in which a large proportion of gas by volume is dispersed in a liquid. 
3.7  
lot 
all containers of the same size, shape, and design manufactured from the same raw materials under similar 
condition of production in a consignment 
3.8 
non-ionic surface active agent 
surface active agent which does not produce ions in an aqueous solution. The solubilizing in water is due to 
the presence in molecule of functional groups which have a strong affinity for water. 
3.9  
soap 
an anionic surface active agent which exhibits the phenomenon of reversible hydrolysis by action of water. 
Their reaction is alkaline. 
3.10 
solubilizing power 
effectiveness of a dissolving surface active agent to confer on certain bodies of low solubility in the pure 
solvent, an apparent solubility by micelle formation. 
4 Requirements 
4.1 General requirements 
4.1.1 The detergent shall be a clear, homogeneous liquid. It shall remain stable after having been 
maintained at 5 °C for 24 h and shall remain clear, homogeneous fluid when maintained for a further 24 h at 
30 °C. 
4.1.2  The detergent shall be free from visible impurities, and objectionable odour. It shall contain synthetic 
organic active matter and foam stabilizing agents. It shall be completely miscible with water, and contain no 
soap.  
4.1.3 It shall be an aqueous solution of secondary alkyl sulphate or alkylaryl sulphate, or both, with or 
without not more than one-third of the supplementary surface active agents of alkyl ether sulphate, 
alkanolamide ethylene oxide or linear alcohol ethylene oxide condensate type. A solubilizer such as, toluene 
sulphonate or xylene sulphonate and a foam stabilizer of the alkanolamide type may be added.  
4.1.4 The active ingredients used shall be biodegradable.  
4.1.5 The detergent shall have good cleaning and lathering properties.  
4.1.6 All the substances used in the synthetic organic liquid detergent shall comply with the requirements of 
all parts of EAS 377. FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  3 
 4.2 Specific quality requirements 
The detergent shall also comply with the specific quality requirements given in Table 1 when tested in 
accordance with the corresponding test method. 
Table 1 ― Specific quality requiremen ts for liquid detergent for household use  
S/N Characteristics  Requirements  Test method  
General purpose  Dishwas hing  
i Solubility in water  Completely soluble  Completely soluble  Annex A  
ii Rinsing properties  To pass the test  To pass the test  Annex B  
iii Active ingredient content , % m/m, 
min. 5 12 Annex C  
iv pH at  27 °C of 1 % solution, v/v  6.5 - 11.5 6 - 12 Anne x D 
v Total non  detergent organic 
matter, % m/m, max.  0.5 0.5 Annex E  
 
5 Storage 
When stored in its original sealed, clean and dry container under room temperature (25 ˚C ± 2 ˚C) for a period 
of not less than 12 months from the date of delivery, the material shall retain the properties described in this 
standard. 
6 Packaging and marking   
6.1 Packaging 
The product shall be packed in a suitable, well-closed container, to protect the integrity of the product during 
transportation and sale. 
6.2 Marking 
The container shall be marked legibly and indelibly with the following information: 
a) name of the product as "General purpose or dishwashing synthetic organic liquid detergent"; 
b) manufacturer’s name and physical address;  
NOTE The name, physical address of the distributor/supplier and trade mark may be added as required. 
c) batch or code number; 
d) net weight; 
e) country of origin; 
f) instructions for use (which shall be in either English, Kiswahili or French or in combination as agreed 
between the manufacturer and supplier”) ; 
g) date of manufacture and best before date. FOR COMMENTS ONLYEAS 383: 2013 
4 © EAC  2013  – All rights reserved  
 7  Sampling  
Sampling shall be done in accordance with Annex F. 
8 Criteria for conformity 
The lot shall be deemed to comply with the requirements of this standard if, after inspection and testing, the 
requirements of Clause 4 are satisfied.  FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  5 
 Annex A 
(normative) 
 
Test for solubility 
A.1 Preparation of synthetic hard water  
Weigh to the nearest 0.001 g, about 0.264 g of CaCl 2.2H 2O and 0.295 g of MgSO 4. 2H 2O. Transfer 
quantitatively to a 1-L volumetric flask; dissolve in a small portion of distilled water and make up to the mark 
with distilled water. 
The resulting solution will have a concentration of 8.1 millimole per litre calcium hardness. 
A.2 Procedure 
Using a pipette, transfer 5.0 mL of the detergent into a test tube and add sufficient synthetic hard water 
prepared in A.1 to give a volume of 50 mL. Stir vigorously for 5 min, and observe for solubility. 
 FOR COMMENTS ONLYEAS 383: 2013 
6 © EAC  2013  – All rights reserved  
 Annex B 
(normative) 
 
Test for rinsing properties  
Dissolve 2.0 m L of the liquid detergent as completely as possible in 98 mL of synthetic hard water (see A.1) at 
ambient temperature, in a clean 250-mL Erlenmeyer flask. Stopper the flask and stir vigorously for 1 min. Pour 
out the solution. Rinse the flask by the same procedure, using three 75 mL portions of synthetic hard water 
alone. Invert the flask, allow to dry and examine for any residue not rinsed from the interior. The flask shall 
contain no more residues after being dried than a similar allowed drying after rinsing with synthetic hard water 
alone. 
 FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  7 
 Annex C 
(normative) 
 
Methods of test for synthetic detergents 
C.1 Quality of reagents 
Unless otherwise specified, chemicals of analytical grade and water, distilled quality, shall be employed in all 
the tests. 
C.2 Qualitative identification of non-soapy detergents 
C.2.1 General 
It is recommended that the quantities examination of a sample is preceded by a qualitative identification of the 
type of non-soapy detergent present. The procedure given in C.2.4 permits ascertaining whether the material 
is based on soap or a non-soapy detergent. The method described in C.2.5 enables the identification of the 
type of non-soapy detergent, that is, whether it is cationic, anionic or non-ionic. 
C.2.2 Apparatus 
C.2.2.1 Measuring cylinder,  100 m L capacity; glass-stoppered  
C.2.2.2 Tests tubes 
C.2.3 Reagents 
C.2.3.1 Hydrochloric acid , 5 % (m/v) solution 
C.2.3.2 Methyl orange indicator solution , 0.1% (m /v) solution 
C.2.3.3 Methylene blue reagent  — Dissolve 0.5 g of methylene blue in distilled water and make up the 
volume to 100 mL. To 6 mL of this solution, add 120 mL of one mol sulphuric acid and 50 g of anhydrous 
sodium sulphate, and make up the volume to one litre with distilled water. 
C.2.3.4 Chloroform 
C.2.3.5 Cetyl dimethyl benzyl ammonium chloride solution , 0.2 %  (m/v) solution 
C.2.4 Procedure for identification of soapy and non-soapy detergents 
Take about 0.1 g of the sample in a test- tube, add about 20 mL of water and shake well until dissolution is 
complete. Add a drop of methyl orange indicator solution, and make it just acidic by adding a few drops of 
hydrochloric acid solution. If the lather is destroyed and fatty acids separate out, then the material is based on 
a soap. If the lather persists, then the active matter is non-soapy detergent. 
C.2.5 Procedure for identification of the type non-soapy detergents  
C.2.5.1 Dissolve about 0.1 g of the sample in about 20 mL of water, and take 10 mL of the solution in a 
measuring cylinder. Add 10 mL of methylene blue reagent and 15 mL of chloroform, shake well and allow to 
stand, Observe whether the colour is in the chloroform layer of the aqueous layer. FOR COMMENTS ONLYEAS 383: 2013 
8 © EAC  2013  – All rights reserved  
 C.2.5.2 If the colour is initially in the chloroform layer, add 0.1 mL of cetyl dimethyl benzyl ammonium 
chloride solution, and shake well and allow to stand. If the colour is regained in the chloroform layer, the active 
matter is anionic. If the colour is transferred to the aqueous layer, the active matter is non-ionic. 
C.2.5.3 If the colour is initially in the aqueous layer, add 0.1 mL of sodium lauryl sulphate solution, and 
shake well and allow to stand. If the colour is retained in the aqueous layer, the active matter is cationic. If the 
colour is transferred to the chloroform layer, the active matter is non-ionic. 
C.3 Quantitative identification of active ingredient 
This shall be carried according to ISO 2271. FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  9 
 Annex D 
(normative) 
 
Determination of pH 
D.1 General 
pH determination should be made in an acid free atmosphere.  
D.2 Apparatus 
D.2.1 Any standard pH meter , equipped with a low sodium error glass electrode. The instrument shall be 
calibrated and standardized with standard buffer solutions (see D.3.2) before use. 
D.2.2 Volumetric flask , 1000-mL capacity 
D.2.3 Beakers , 1000-mL 
D.3 Reagents 
D.3.1 Distilled water shall be boiled thoroughly or purged with carbon dioxide-free air to remove carbon 
dioxide and shall be protected with soda lime or soda asbestos while cooling and in storage. The pH of this 
water shall be protected with soda lime or soda asbestos while cooling and in storage. The pH of this water 
shall be between 6.2 and 7.2 at 27 °C. The residue on evaporation when heated at 105 °C for one hour shall 
not exceed 0.5 mL per litre. 
D.3.2 Standard buffer solutions with the pH range of 9 to 11 at 27 °C for calibrating the pH meter.  
D.4 Procedure 
Weigh to the nearest milligram approximately 10 g of the material and transfer to a 1-L volumetric flask. 
Partially fill the flask with distilled water and agitate until the sample is completely dissolved. Adjust the 
temperature of the solution and the distilled water to 27 °C ± 2 °C and fill to the calibration mark with distilled 
water, stopper the flask mix thoroughly and allow the solution to stand at a temperature of 27 °C ± 2 °C for two 
hours prior to measuring the pH. Measure the pH of the solution at 27 °C ± 2 °C using a glass electrode. FOR COMMENTS ONLYEAS 383: 2013 
10 © EAC  2013  – All rights reserved  
 Annex E 
(normative) 
 
Determination of non-detergent organic matter 
E.1 General 
The term non-detergent organic matter includes hydrocarbons, fatty alcohols and perfumes. Using petroleum 
ether and under the conditions prescribed, non-detergent organic matter only is extracted leaving any 
alkylolamide present in the material. 
E.2 Apparatus 
E.2.1 Evaporating basin 
E.2.2 Separating funnels , 1 000-mL capacity. 
E.2.3 Wide mouthed flat-bottomed flask , 200-mL capacity 
E.2.4 Buchner flask , 500-mL capacity, fitted with a sintered glass filter funnel (porosity 4) 
E.3 Reagents 
E.3.1 Ethyl alcohol, 50 %, 70 %. 90 % and 96% (by volume) 
E.3.2 Petroleum ether,  boiling range 40 °C to 60 °C non-volatile residue at 80 °C maximum 0.001% 
E.3.3  Acetone,  non-volatile residue at 80 °C maximum 0.001 % 
E.4 Procedure  
E.4.1 For the removal of inorganic salts, weigh accurately about 5 g of the material in a 150-mL squat 
beaker. Extract with 50 mL of hot 90 % ethanol by heating on the steam bath for about 2 min stirring and 
breaking up any hard lumps with a glass rod flattened at the end. 
Allow the solid matter to settle and decant the hot aIcoholic solution through a sintered glass filter funnel 
(porosity 4) fitted to a 500-mL Buchner flask to which suction is applied. Repeat the extraction in a similar 
manner with five further consecutive 30-mL quantities of boiling 90 % ethanol. Pass each extract in turn 
through the filter into the flask. 
E.4.2  Transfer quantitatively all the combined filtrate from the Buchner flask to a 1-L separating funnel and 
rinse the flask four times with 40-mL quantities of distilled water, transferring each wash in turn to the 
separating funnel. Add 100 mL of petroleum ether, swirl gently to ensure adequate mixing and allow the two 
phases to separate. Run off the aqueous alcoholic layer into a second separating funnel, and extract with 75 
mL of petroleum ether. Repeat the extraction of the aqueous alcoholic phase in the third separating funnel 
with a further 75 mL of petroleum ether. Combine the three ether extracts in the first separating funnel. Rinse 
each of the two empty funnels with a few millilitres petroleum ether and add the rinsing to the combined ether 
extracts. FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  11 
 E.4.3  Wash the combined ether extracts and rinsing (see E.4.2) with four successive 50-mL portions of 70 
% ethyl alcohol, shaking and removing the alcoholic phase each time. Transfer the ether layer in stages to a 
tared flask and evaporate off the solvent. Add 10 mL of acetone and evaporate off the solvent. Rotate the 
flask on a steam bath during the operation. Cool the flask to about 60 °C to 65 °C, gently blow out the last 
traces of solvent with a current of dry air, cool in a desiccator and weigh. 
E.5 Calculation 
The n on- detergent organic matter shall be expressed as follows: 
Non- detergent organic matter, %, by mass = 
mm1100  
where  
m1 is the mass, in grams, of the non-detergent organic matter in the flask; and 
m is the mass, in grams, of the material taken for the test. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 FOR COMMENTS ONLYEAS 383: 2013 
12 © EAC  2013  – All rights reserved  
 Annex F 
(normative) 
 
Sampling  
F.1 Procedure  
F.1.1 In a single consignment, all packages (cartons) containing toilet soap cakes drawn from the same 
batch of production shall constitute a lot. For ascertaining the conformity of the lot to the requirements of this 
standard, tests shall be carried out on each lot separately. The number of packages to be selected for drawing 
the sample shall be in accordance with Table F.1 .  
Table F.1 — Scale of sampling 
Number  of packages (cartons) in 
the lot  
N Number of packages (cartons) to be 
selected  
n Number  of samples  
4 to 15  3 3 
16 to 40  4 4 
41 to 65  5 2 
66 to 110  7 2 
111 and above  10 1 
 
F.1.2 The packages shall be selected at random, using tables of random numbers. If these are not 
available, the following procedure shall be applied:  
Starting from any package, count all the packages in one order as 1, 2, 3.... N, selecting every k th package, 
where k is the integral part of N  n.  
F.1.3 From each package thus selected, draw at random an equal number of cakes so as to obtain a total 
mass of at least 2 kg.  
F.2 Preparation of test samples 
F.2.1 Composite sample  
Weigh each cake separately (including any material that may have adhered to the wrapper), and calculate the 
average mass. Cut each of the remaining cakes into eight parts by means of three cuts at right angles to each 
other through the middle. Grate finely the whole of two diagonally opposite eighths of each specimen. Mix the 
gratings and place in a clean, dry, airtight glass container.  
F.2.2 Samples for testing 
Immediately after preparation of composite sample (F.2.1), take at one time all test samples required for the 
tests in 4.2. Weigh out the test sample required for determination of free alkali or acid content, and use it 
immediately.  
 FOR COMMENTS ONLYEAS 383: 2013 
© EAC 2013  – All rights reserved  13 
  FOR COMMENTS ONLYFOR COMMENTS ONLYEAS 383: 2013    
 
© EAC 2013 – All rights reserved