ICS 71.100.70 
 
Reference number  
DRS 489-3: 2022 
© RSB 2022 
  
 RWANDA 
STANDARD  DRS 
489-3  
 
First edition  
2022-mm-dd 
 
Oils for cosmetic use — Specification  
Part 3: Passion fruit (Maracuja) seed oil  
   
DRS 489-3: 2022 
 
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 In order to match with technological development and to keep continuous progress in industries, 
standards are subject to periodic review. Users shall ascertain that they are in possession of the latest 
editio n 
 
 
 
 
 
 
© RSB 2021  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized 
in any form or by any means, electronic or mechanical, including photocopying and microfilm, without 
prior written permission from R SB. 
Requests for permission to reproduce this document should be addressed to : 
Rwanda Standards  Board  
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Toll Free: 3250  
E-mail: info@rsb.gov.rw  
Website: www.rsb.gov.rw  
ePortal: www.portal.rsb.gov.rw   DRS 489-3: 2022 
 
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 Contents  Page 
 
 
1 Scope  ................................ ................................ ................................ ................................ .............  1 
2 Normative references  ................................ ................................ ................................ ...................  1 
3 Terms and definitions  ................................ ................................ ................................ ..................  2 
4 Requirements  ................................ ................................ ................................ ................................  2 
4.1 General requirements  ................................ ................................ ................................ ..................  2 
 
4.2 Specific requirements  ................................ ................................ ................................ ..................  3 
5 Packag ing ................................ ................................ ................................ ................................ ...... 4 
6 Labelling  ................................ ................................ ................................ ................................ ........  5 
7 Sampling  ................................ ................................ ................................ ................................ ....... 5 
Annex  A (normative)  Determination of critical solution temperature  ................................ .................  6 
A.1 General  ................................ ................................ ................................ ................................ ..........  6 
A.2 Procedure  ................................ ................................ ................................ ................................ ...... 6 
 
 
 DRS 489-3: 2022 
 
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 Foreword   
Rwanda Standard s are prepared by Technical Committees and approved by Rwanda Standards Board (RSB) 
Board of Directors in  accordance with the procedures of RSB,  in compliance with Annex 3 of the WTO/TBT 
agreement on the preparation, adoption and application of standards.  
The main task of technical committees is to prepare national standards. Final Draft Rwanda Standards adopted 
by technical committees are ratifie d by members of RSB Board of Directors for publication and gazettment as 
Rwanda Standards.  
DRS 489-3 was prepared by Technical Committee RSB/TC 011, Cosmetics and related products . 
DRS 489 consists of the following parts, under the general title Oils for cosmetic use — Specification : 
— Part 1:  Baobab seed oil  
 
— Part 2:  Chia seeds oil  
 
— Part 3: Passion fruits (maracuja) seed oil  
Committee membership  
The following organizations were represented on the Technical Committee on Cosmetics and related products   
(RSB/TC 011) in the preparation of this standard.  
University of Rwanda -College of Science and Technology (UR -CST)  
Rwanda Food and Drugs Authority (R wanda -FDA)  
Rwanda Inspectorate, Competition and Consumer protection Authority (RICA)  
Rwanda Forensic Laboratory (RFL)  
Standards for Sustainability (SFS)  
SULFO Industries Rwanda  
ORIBUT Company Ltd  
Divine Hope Company Ltd  DRS 489-3: 2022 
 
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 Better Home Ltd  
Beauty Makers Association (BMA)  
Rwanda Standards Board (RSB) – Secretariat  DRS 489-3: 2022 
 
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 Introduction   
Passion fruit ( Passiflora edulis)  commonly known as  maracuja is a popular fruit that is mainly used to make 
juices and desserts. Beyond this, however, the seeds are also known as a rich source of lipids, proteins, 
minerals, fiber, and a number of bioactive compounds such as sterols, tocols, and carotenoids. Consequently, 
the seeds have gained increasing interest as a feedstock of oil for cosmetic and nutritional purposes.  
Passion fruit (Maracuj a) seed  oil is rich in unsaturated fatty acids with nutritional and moisturizing properties for 
the skin and hair. It can be used in formulations of gels, tonics, creams, lotions, soaps, sunscreens, makeup 
and in formulations that seek a dry and a velvety touch to the skin. For the treatment of hair, it  is use d in products 
such as shampoo, conditioner, capillary mask, boosters, and leave -in products, in order to improve features 
such as hydration and gloss.DRS 489-3: 2021  
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 Oils for cosmetic us  — Specification — Part 3: Passion fruit s (Maracuja ) 
seed  oil 
1 Scope  
This Draft Rwandan Standard specifies the requirements, sampling and test methods for passion fruit s 
(maracuja ) seeds oil for cosmetic use. 
2 Normative references  
The following documents are referred to in the text in  such a way that some or all of  their content constitutes 
requirements of this document.  For dated references, only the edition  cited applies. For undated references, the 
latest edition of the referenced document (including any amendments) applies.  
RS EAS 346, Labelling of cosmetics — General requirements  
 RS EAS 846, Glossary of terms relating to the cosmetic industry   
RS EAS 847 -2, Cosmetics — Analytical methods — Part 2: Determination of moisture content and volatile 
matter content   
RS EAS 847 -5, Cosmetics — Analytical methods — Part 5: Determination of unsaponifiable matter ,                     
 
RS EAS 847 -7, Cosmetics — Analytical methods — Part 7: Determination of specific gravity  
RS EAS 847 -9, Cosmetics — Analytical methods — Part 9: Determina tion of colour  
RS EAS 847 -10, Cosmetics — Analytical methods - Part 10: Determination of acetyl value and hydroxyl value  
RS EAS 847 -12, Cosmetics — Analytical methods - Part 10: Determination  of flash point by Pensky — Martens 
closed cap tester  
RS EAS 84 7-13, Cosmetics — Analytical methods — Part 13: Determination of rancidity  
RS EAS 847 -16, Cosmetics — Analytical methods — Part 16: Determination of lead, mercury and arsenic 
content                                                                                                                                                                                   
RS ISO 660,  Animal  and vegetable fats and oils — Determination of acid value and acidity                                            
RS ISO 663, Animal a nd vegetable fats and oils — Determination of insoluble impurities content  DRS 489-3: 2021  
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 RS ISO 3657,  Animal and vegetable fats and oils — Determination of saponification value                                            
RS ISO 3961, Animal and vegetable fats and oils — Determination of iodine value  RS ISO 6320 Animal  and 
vegetable fats and oils — Determination of refractive index                                                                                                                      
RS ISO 3960 Animal  and   vegetable fats and oils — Determination of peroxide value  — Iodometric (visual) 
endpoint determination    
RS 278, Cosmetics — Methods of sampling  
 RS ISO 6887 -1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension 
and decimal dilutions for microbiological examination — Part 1: General rules for preparation of the initial 
suspensions and decimal dilutions  
3 Terms and defin itions  
For the purposes of this standard, the terms and definitions given in RS EAS 846 and the following apply.  
3.1 
passion fruit  (maracuja)  seeds oil  
an oil obtained from the seed s of passion fruit/maracuja plant (Passiflora edulis ), by a cold pressing or solvent 
extraction  which yields to yellow to vibrant yellow  in colour and very pleasant  in odour  
4 Requirements  
4.1 General requiremen ts 
4.1.1  The product shall be insoluble in water but soluble in aromatic hydrocarbon solvents, vegetable and 
mineral oil.  
4.1.2  When examined visually, the product shall be:  
a) clear;  
b) free from sediments and other foreign matter; and  
c) free from separated water, added colouring and flavouring substances.  DRS 489-3: 2021  
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 4.1.3  The product shall be free from admixture with other oils.  
4.2 Specific requirements  
4.2.1  The product shall comply with the requirements given in Table 1 when tested in accordance with the 
methods specified therein.  
Table 1 — Requirements for passion fruit  seeds oil /maracuja  oil for cosmetic industry  
S/N Characteristics  Requirements  Test method  
i.  Moisture content, % m/m, max  7.30-7.45 RS EAS 847 -2 
ii.  Colour in a 1” cell on the Lovibond scale, expressed 
as Y + 5R, max. deepness  4.0 RS EAS 847 -9 
iii.  Insoluble impurities, % m/m, max.  0.25 RS ISO 663  
iv.  Refractive index at 40 C, range  1.468 -1.472  RS ISO 6320  
v.  Specific gravity at 20 C 0.905-0.988  RS EAS 847 -7 
vi.  Saponification value  187-193 RS ISO 3657  
vii.  Iodine value  124-132 RS ISO 3961  
viii.  Peroxide value, mg/kg, max  1.43- 1.49 RS ISO 3960  
ix.  Acid value, max.  2.28-2.41 RS ISO 660  
x.  Unsaponifiable matter, % m/m, max  1.42-1.60 RS EAS 847 -5 
xi.  Flash point, C min  290 RS EAS 847-12 
xii.  Test for rancidity  Shall be free from rancidity  RS EAS 847-13 
xiii.  Critical solution temperature, 0ºC, max  0 Annex A  
 
 
 
 
 
 DRS 489-3: 2021  
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 4.2.2  The products shall comply with the limits for heavy metal contaminants in accordance with Table 2, 
when tested in accordance with the methods specified therein.  
Table 2⎯  Limits for heavy metal contaminants for passion fruit/maracuja seed s oil 
S/N Characteristics  Maximum limit, mg/kg  Test method  
i.  Lead  10 
RS EAS 847 -16 ii.  Arsenic  2  
iii.  Mercury  2  
- The total amount of heavy metals as lead, mercury and arsenic, in combination, in the finished 
product should not exceed 10 mg/kg.  
- The heavy metals including lead, mercury and arsenic may be as a result of contamination 
during processing and should no t be deliberately added as ingredients.  
 
4.2.3  The products shall also comply with the microbiological limits given in Table 3 when tested in 
accordance with the methods specified therein.  
Table 3 ⎯ Microbiological limits for passion fruit/maracuja seed oil  
Characteristics  Maximum limit  Test method  
Total viable count for aerobic mesophyllic micro -
organisms per gram.  100 RS ISO 6887 -1 
Pseudomonas aeruginosa  Not detectable in 0.5 g  RS ISO 22717  
Staphylococcus aureus  RS ISO 22718  
Candida albicans  RS ISO 18416  
 
5 Packaging  
The product shall be packaged in suitable well -sealed containers that shall protect the contents and shall not 
cause any contamination or react with the products . DRS 489-3: 2021  
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 6 Labelling  
In addition to the requirements of : 
6.1  Labelling shall be done in accordance with RS EAS 346  
6.1.1  the words “passion fruit  (maracuja ) seed oil ”” and ‘For external use only’ shall be conspicuously marked, 
either printed on the label affixed to the container, or lithographed, or stencilled thereon with indelible ink.  
7 Sampling  
Random samples of the product shall be drawn for test in accordance with RS 278 from the market, factory or 
elsewhere.  
 
. DRS 489-3: 2021  
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 Annex  A 
(normative)  
 
Determination of critical solution temperature  
A.1 General  
The reagent shall be prepared by diluting ethyl alcohol or rectified spirit with distilled water till the relative density 
of the mixture at 15.5 ºC is 0.8303 ± 0.0001, when compared with distilled water at the same temperature. 
Denatur ed alcohol shall not be used for this test.  
A.2 Procedure  
Mix in a test tube, 1.0 g of the oil, with 4.15 times its mass of the reagent. Upon examination, the solution thus 
obtained shall be perfectly clear at 20ºC and shall remain clear when cooled and maint ained for 5 minutes at a 
temperature of 0 ºC.  
  DRS xxx: 2021  
 
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