II
(Non-legislative acts)
REGUL ATIONS
COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/1490 
of 1 March 2022
concer ning the author isation of expressed lemon essential oil, residual fraction of expressed lemon 
oil distilled, distilled lemon essential oil (volatile fraction) and distilled lime essential oil as feed 
additiv es for certain animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation. Article 10(2) of that Regulation provides for the 
re-evaluation of additives author ised pursuant to Council Directive 70/524/EEC (2).
(2) Expressed lemon essential oil, residual fraction of expressed lemon oil distilled, distilled lemon essential oil (volatile 
fraction) and distilled lime essential oil were author ised without a time limit in accordance with Directive 
70/524/EEC as feed additives for all animal species. These additives were subsequently entered in the Registe r of 
feed additives as existing products, in accordance with Article 10(1)(b) of Regulation (EC) No 1831/2003.
(3) In accordance with Article 10(2) of Regulation (EC) No 1831/2003 in conjunction with Article 7 thereof, an 
application was submitt ed for the re-evaluation of expressed lemon essential oil, residual fraction of expressed 
lemon oil distilled, distilled lemon essential oil (volati le fraction) and distilled lime essential oil for all animal species.
(4) The applicant requested expressed lemon essential oil, residual fraction of expressed lemon oil distilled, distilled 
lemon essential oil (volatile fraction) and distilled lime essential oil to be author ised also for use in water for 
drinking. However , Regulation (EC) No 1831/2003 does not allow the author isation of ‘flavouri ng compo unds ’ for 
use in wate r for drinking. Theref ore, the use of expressed lemon essential oil, residual fraction of expressed lemon 
oil distilled, distilled lemon essential oil (volati le fraction) and distilled lime essential oil in wate r for drinking should 
not be allowed.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Council Directive 70/524/EEC of 23 November 1970 concer ning additives in feedingstuffs (OJ L 270, 14.12.1970, p. 1).EN Offi cial Jour nal of the European Union 9.9.2022 L 234/1  (5) The applicant requeste d the additives to be classified in the additive cate gory ‘sensor y additives ’ and in the functional 
group ‘flavouring compo unds ’. That application was accompan ied by the particulars and documents required under 
Article 7(3) of Regulation (EC) No 1831/2003.
(6) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 18 March 2021 (3)that, under the 
proposed conditions of use of expressed lemon essential oil, residual fraction of expressed lemon oil distilled, 
distilled lemon essential oil (volati le fraction) and distilled lime essential oil do not have adverse effects on animal 
health, consumer health or the environment. The Author ity concluded that pets (com panion animals and 
ornamental fish) are not normally exposed to expressed lemon essential oil, residual fraction of expressed lemon oil 
distilled and distilled lime essential oil, theref ore, no conclusion can be drawn for those species. Consequently , those 
additives cannot be author ised for those species. The Authority also concluded that expressed lemon essential oil, 
residual fraction of expressed lemon oil distilled, distilled lemon essential oil (volati le fraction) and distilled lime 
essential oil should be considered as skin sensitisers and as irritants to skin and eyes and the respirator y tract and 
also that expressed lemon oil and its residual fraction contain furocoumar ins that may cause photot oxicity . 
Theref ore, the Commission considers that appropr iate prote ctive measures should be take n to prevent adverse 
effects on human health, in particular as regards the users of the additive.
(7) The Author ity concluded, that expressed lemon essential oil, residual fraction of expressed lemon oil distilled, 
distilled lemon essential oil (volati le fraction) and distilled lime essential oil are recognised to flavour food and their 
function in feed would be essentially the same as that in food, and theref ore, no further demonstration of efficacy is 
considered necessar y. It also verified the repor t on the methods of analysis of the feed additives in feed submitte d by 
the Reference Laboratory set up by Regulation (EC) No 1831/2003.
(8) The assessment of expressed lemon essential oil, residual fraction of expressed lemon oil distilled, distilled lemon 
essential oil (volatile fraction) and distilled lime essential oil shows that the conditions for author isation, as provided 
for in Article 5 of Regulation (EC) No 1831/2003, are satisfied. According ly, the use of these substances should be 
author ised as specif ied in the Annex to this Regulation.
(9) The fact that expressed lemon essential oil, residual fraction of expressed lemon oil distilled, distilled lemon essential 
oil (volati le fraction) and distilled lime essential oil are not author ised for use as a flavouring in water for drinking, 
does not preclude their use in compo und feed which is administe red via wate r.
(10) Since safet y reasons do not require the immediate application of the modifications to the conditions of author isation 
of the substance concer ned, it is appropr iate to allow a transitional period for interest ed parties to prepare 
themselves to meet the new requirements resulting from the author isation.
(11) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Author isation
The substances specifi ed in the Annex, belonging to the additive cate gory ‘sensor y additives ’ and to the functional group 
‘flavouring compounds’, are author ised as additives in animal nutr ition, subject to the conditions laid down in that Annex.
(3)EFSA Jour nal 2021;19(4):6548.EN Offi cial Jour nal of the European Union L 234/2 9.9.2022  Article 2
Transitional measures
1. The substances specifi ed in the Annex and premixtures containing these substances, which are produced and labelled 
before 29 March 2023 in accordance with the rules applicable before 29 September 2022 may continue to be placed on the 
mark et and used until the existing stoc ks are exhaust ed.
2. Com pound feed and feed mat erials containing the substances as specifi ed in the Annex which are produced and 
labelled before 29 Sept ember 2023 in accordance with the rules applicable before 29 Sept ember 2022 may continue to be 
placed on the market and used until the existing stoc ks are exhaust ed if they are intended for food-producing animals.
3. Com pound feed and feed mat erials containing the substances as specifi ed in the Annex which are produced and 
labelled before 29 Sept ember 2024 in accordance with the rules applicable before 29 Sept ember 2022 may continue to be 
placed on the market and used until the existing stoc ks are exhaust ed if they are intended for non-f ood-producing animals.
Article 3
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 1 March 2022
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 9.9.2022 L 234/3  ANNEX 
Identification 
number of the 
additiveName of 
the holder 
of 
author isa-
tionAdditiveCompo sition, chem ical formula, 
descr iption, analytical methodSpecies or categor y 
of animalMaximum 
ageMinimum 
contentMaximum 
content
Other provisionsEnd of period of 
author isation mg active substance/kg of 
complet e feed with a 
moisture content of 12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b139-eo - Expressed 
lemon 
essential oilAdditive composition
Expressed lemon essential oil from 
fruit peel of Citrus limon (L.) Osbeck
Liquid formChick ens for 
fattening- - 35 1.The additive shall be incor po­
rated into the feed in the form 
of a premixture.
2.In the directions for use of the 
additive and premixtures, the 
stora ge conditions and stabi ­
lity to heat treatment shall be 
indicated .
3.Mixture of expressed lemon 
essential oil with other bota ­
nical additives is permitted 
provid ed that the amounts 
of perillaldeh yde, furocou ­
mar ins, and metho xycou­
mar ins in feed mat erials and 
compo und feed are lower 
than the one resulting from 
the use of a sing le additive at 
the maximum or recom ­
mended level for the species 
or animal cate gory.
4.For users of the additive and 
premixtures, feed business 
operat ors shall establish op­
erational procedures and or­
gani sational measures to ad­
dress pote ntial risks by 
inhalation, dermal contact 29 Septembe r 
2032
Turkeys for 
fattening
Salmonids40
Char acter isation of the active substance
Expressed lemon essential oil 
obtained by cold expression from 
fruit peel of Citrus limon (L.) Osbeck 
as defined by the Council of 
Europe (1)
d-Limonene: 60–73 %
β-Pinene (pin-2(10)-ene): 9–18 %
γ-Terpinene: 6–12 %
α-Pinene (pin-2(3)-ene): 1,3–3,0 %
Sabinene (4(10)-thujene): 0,3–3,0 %
Geranial: 0,1–2,0 %
Neral: 0,1–1,8 %
Perillaldeh yde: ≤ 0,023 %
Furocoumar ins: ≤ 0,3 %
Metho xycoumar ins: ≤ 0,06 %Laying hens 52
Pigs for fattening 74
Piglets 62
Sows 92
Calves (milk 
replacers)
Cattle for 
fattening
Dair y cows90EN Offi cial Jour nal of the European Union L 234/4 9.9.2022  or eyes contact. Where those 
risks cannot be eliminated or 
reduced to a minimum by 
such procedures and mea ­
sures, the additive and pre­
mixtures shall be used with 
personal prot ective equip ­
ment, including skin, eye 
and breathing protection.CAS number 84929-31-7
FEMA number 2625
CoE number: 139
Analytical method (2)
For the quantification of 
phytochemical marker d-limonene in 
the feed additive or in mixture of 
flavouring compo unds:
— Gas chromat ography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 855)Horses 137
Sheep/Goats
Rabbits30
2b139- rf - Residual 
fraction of 
expressed 
lemon oil 
distilledAdditive composition
Residual fraction of expressed 
lemon oil distilled from fruit peel of 
Citrus limon (L.) Osbec k
Liquid formChick ens for 
fattening- - 11 1.The additive shall be incor po­
rated into the feed in the form 
of a premixture.
2.In the directions for use of the 
additive and premixtures, the 
stora ge conditions and stabi ­
lity to heat treatment shall be 
indicated .
3.Mixture of residual fraction of 
expressed lemon oil distilled 
with other botanical additives 
is permitted provid ed that the 
amounts of perillaldeh yde, 
furocoumar ins, and metho x­
ycoumar ins in feed mat erials 
and compo und feed are lower 
than the one resulting from 
the use of a sing le additive at 
the maximum or recom ­
mended level for the species 
or animal cate gory.29 Septembe r 
2032
Laying hens
Turkeys for 
fatening
Rabbits
Salmonids12
Char acter isation of the active substance
Residual fraction of expressed 
lemon oil distilled obtained by the 
distillation of lemon essential oil 
expressed from fruit peel of Citrus 
limon (L.) Osbeck
The specif ications of the active 
substance are:
d-Limonene 
5989-27-5 01.045 51–63 53.3 49.­
.3–56.8
c-Terpinene 
99-85-4 01.020 8–17 16.9 12.8–23 ­
23.3Ruminants 20
Piglets 20
Pigs for fattening 24
Sows 30
Horses 35EN Offi cial Jour nal of the European Union 9.9.2022 L 234/5  4.For users of the additive and 
premixtures, feed business 
operat ors shall establish op­
erational procedures and or­
gani sational measures to ad­
dress pote ntial risks by 
inhalation, dermal contact 
or eyes contact. Where those 
risks cannot be eliminated or 
reduced to a minimum by 
such procedures and mea ­
sures, the additive and pre­
mixtures shall be used with 
personal prot ective equip ­
ment, including skin, eye 
and breathing protectionGeranial 
141-27-5 05.188 6–12 10.4 9.5–11 ­
11.2
Neral 
106-26-3 0.8724 5–9 7.8 6.2–8.9
b-Pinene (pin-2(10)-ene) 
127-91-3 01.003 0.3–5.5 1.24 0.3­
32–3.38
b-Bisabolene 
495-61-4 01.028 0.3–4
d-Limonene: 51–63 %
γ-Terpinene: 8–17 %
Geranial: 6–12 %
Neral:5–9 %
β-Pinene (pin-2(10)-ene): 0,3–5,5 %
β-Bisabolene: 0,3–4 %
Perillaldeh yde: ≤ 0,092 %
Furocoumar ins: ≤ 0,8 %
Metho xycoumar ins: ≤ 0,22 %
CoE number: 139
Analytical method (2)
For the quantification of 
phytochemical marker d-limonene in 
the feed additive or in mixture of 
flavouring compo unds:
— Gas chromat ography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 855)EN Offi cial Jour nal of the European Union L 234/6 9.9.2022  2b139-di - Distilled 
lemon 
essential oil 
(volati le 
fraction)Additive composition
Distilled lemon essential oil (volatile 
fraction) obtained from lemon 
essential oil expressed, from fruit 
peel of Citrus limon (L.) Osbeck
Liquid formChick ens for 
fattening- - 36 1.The additive shall be incor po­
rated into the feed in the form 
of a premixture.
2.In the directions for use of the 
additive and premixtures, the 
stora ge conditions and stabi ­
lity to heat treatment shall be 
indicated .
3.Mixture of distilled lemon es­
sential oil (volatile fraction) 
with other botanical additives 
is permitted provid ed that the 
amounts of perillaldeh yde, 
furocoumar ins, and metho x­
ycoumar ins in feed mat erials 
and compo und feed are lower 
than the one resulting from 
the use of a sing le additive at 
the maximum or recom ­
mended level for the species 
or animal cate gory.
4.For users of the additive and 
premixtures, feed business 
operat ors shall establish op­
erational procedures and or­
gani sational measures to ad­
dress pote ntial risks by 
inhalation, dermal contact 
or eyes contact. Where those 
risks cannot be eliminated or 
reduced to a minimum by 
such procedures and mea ­
sures, the additive and pre­
mixtures shall be used with 
personal prot ective equip ­
ment, including skin, eye 
and breathing protection.29 Septembe r 
2032
Laying hens 53
Rabbits 56
Turkeys for 
fattening48
Char acter isation of the active substance
Distilled lemon essential oil (volatile 
fraction) obtained by lemon 
essential oil expressed, from fruit 
peel of Citrus limon (L.) Osbeck as 
defined by the Council of Europe (1)
The specif ications of the active 
substance are:
d-Limonene: 66–78 %
β-Pinene (pin-2(10)-ene): 5–20 %
γ-Terpinene: 1,5–9,5 %
α-Pinene (pin-2(3)-ene): 0,5–3,0 % 
Sabinene: 0,3–3,0 %
Furocoumar ins: ≤ 0,1 mg/kg
Metho xycoumar ins: ≤ 0,1 mg/kg
CoE number: 139Piglets 64
Pigs for fattening 76
Sows 94
Calves (milk 
replacers)
Cattle for 
fattening
Sheep/Goats95
Horses 141
Analytical method (2)
For the quantification of 
phytochemical marker d-limonene in 
the feed additive or in mixture of 
flavouring compo unds:
— Gas chromat ography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 855)Dair y cows 91
Salmonids
Ornamental fish
Dogs60
Cats 30EN Offi cial Jour nal of the European Union 9.9.2022 L 234/7  2b141-eo - Distilled lime 
essential oilAdditive composition
Distilled lime essential oil obtained 
by unpe eled fruits of the plant 
species Citrus aurantiifolia (Chr istm.) 
Swing le
Liquid formChick ens for 
fattening- - 8,5 1.The additive shall be incor po­
rated into the feed in the form 
of a premixture.
2.In the directions for use of the 
additive and premixtures, the 
stora ge conditions and stabi ­
lity to heat treatment shall be 
indicated .
3.Mixture of distilled lime es­
sential oil with other botani ­
cal additives is permitted pro­
vided that the amounts of 
perillaldeh yde, furocoumar ­
ins, and metho xycoumar ins 
in feed mate rials and com ­
pound feed are lowe r than 
the one resulting from the 
use of a sing le additive at the 
maximum or recommended 
level for the species or animal 
catego ry.
4.For users of the additive and 
premixtures, feed business 
operat ors shall establish op­
erational procedures and or­
gani sational measures to ad­
dress pote ntial risks by 
inhalation, dermal contact 
or eyes contact. Where those 
risks cannot be eliminated or 
reduced to a minimum by 
such procedures and mea ­
sures, the additive and pre­
mixtures shall be used with 
personal prot ective equip ­
ment, including skin, eye 
and breathing protection.29 Septembe r 
2032
Laying hens 12,5
Turkeys for 
fatening11
Piglets 15
Char acter isation of the active substance
Distilled lime essential oil obtained 
by steam distillation from unpeeled 
fruits of the plant species Citrus 
aurantiifoli a (Chr istm.) Swing le as 
defined by the Council of Europe (1)
The specif ications of the active 
substance are:
d-Limonene: 45–52 %
γ-Terpinene: 10–14 %
Terpinolene: 5,5–10,5 %
αΤer pineol: 6–8 %
β-Car yophyllene: 0,2–0,8 %
Furocoumar ins: ≤ 0,0083 %
Metho xycoumar ins: ≤ 0,03 %
CoE number:141Pigs for fattening 18
Lactating sows 22
Cattle for 
fattening33,5
Calves (milk 
replacers)35,5
Dair y cows 21,5
Sheep/Goats
Horses33,5
Rabbits 13,5
Salmonids
Ornamental fish30
Analytical method (2)
For the quantification of 
phytochemical marker d-limonene in 
the feed additive or in mixture of 
flavouring compo unds:EN Offi cial Jour nal of the European Union L 234/8 9.9.2022  — Gas chromat ography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 855)
(1)Natural sources of flavourings - Repor t No. 2 (2007).
(2)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN Offi cial Jour nal of the European Union 9.9.2022 L 234/9