COMMISSION IMPLEMENTING DECISION (EU) 2018/320 
of 28 Febr uary 2018 
on certain animal health protection measures for intra-Union trade in salamanders and the 
introduction into the Union of such animals in relation to the fungus Batr achoc hytr ium 
salamandr ivor ans 
(notifi ed under document C(2018) 1208) 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Having regar d to the Treaty on the Functioning of the European Union, 
Having regard to Council Directive 90/425/EEC of 26 June 1990 concer ning veteri nary and zootec hnical chec ks 
applicable in intra-Community trade in certain live animals and products with a view to the compl etion of the intern al 
mark et (1), and in particular Article 10(4) thereof, 
Having regard to Council Directive 91/496/EEC of 15 July 1991 laying down the principles governing the organisation 
of veter inar y checks on animals entering the Community from third countr ies and amending Directives 89/662/EEC, 
90/425/EEC and 90/675/EEC (2), and in particular Article 18(1) thereof, 
Whereas: 
(1)  Since 2013, Batr achochytr ium salamandr ivorans (Bsal), whic h is an emerging pathogen fungus of salamanders, has 
been occur ing in Belgium, German y, the Netherlands and the Unit ed Kingdom. It affects kept and wild 
salamander populations, and it can cause signif icant morbidity and mor tality in those populations. Bsal is lethal 
to certain species of salamanders, while other species are fully or partly resistant to it, but may carry Bsal on their 
skin and theref ore act as a reser voir and source of infection or contamination for other species of salamanders. 
(2)  According to current scientifi c knowle dge concer ning Bsal, compiled by a scientifi c opinion (3) of the European 
Food Safet y Authority (EFSA) about Bsal within its Assessment of listing and cate gorisation of animal diseases in 
the framew ork of Regulation (EU) 2016/429 of the European Parliament and of the Council (4), Bsal seems to be 
endemic at least in Japan, Thailand and Vietnam with around a 3 % prevalence rate in wild salamander 
populations. It is general ly thought to have originated in East Asia and to be widespread and endemic there. At 
the same time, there is a lack of information on how widespread it is in other parts of the world. It is also 
generally thought that trade in infec ted or carrier salamanders contr ibutes to the spread of Bsal. 
(3)  According to available data, both resistant and susceptible salamander species are traded. Council Directive 
92/65/EEC (5) lays down the animal health requirements govern ing trade in and impor ts into the Union of 
animals not subject to the animal health requirements laid down in the specific Union acts listed in Annex F 
thereto. Union animal health legislation, including Directive 92/65/EEC, currently lays down no specifi c animal 
health requirements for trade in salamanders or for their imports into the Union that would be effective in 
prot ecting animal health against the spread of Bsal in the Union. 
(4) The EFSA , in its scientific and technical assistance concer ning the survival, establishment and spread of Batracho ­
chytrium salamandr ivorans (Bsal) in the EU (6) (the EFSA Assistance), assessed the potential of Bsal to affect the 
health of wild and kept salamanders in the Union, the effectiveness and feasibility of a mov ement ban on traded 
salamanders, the validity , reliability and robustness of available diagnostic methods for Bsal detection and possible 
alternative methods and feasible risk mitig ation measures to ensure safe intern ational and intra-Un ion trade in 
salamanders. 5.3.2018 L 62/18 Official Jour nal of the European Union EN     
(1)OJ L 224, 18.8.1990, p. 29. 
(2)OJ L 268, 24.9.1991, p. 56. 
(3)EFSA Journal 2017;15(11):5071 
(4)Regulation (EU) 2016/429 of the European Parliament and of the Council of 9 March 2016 on transmissible animal diseases and 
amending and repealing certain acts in the area of animal health (‘Animal Health Law’) (OJ L 84, 31.3.2016, p. 1). 
(5)Council Directive 92/65/EEC of 13 July 1992 laying down animal health requirements govern ing trade in and impor ts into the 
Community of animals, semen, ova and embr yos not subject to animal health requirements laid down in specific Community rules 
referred to in Annex A (I) to Directive 90/425/EEC (OJ L 268, 14.9.1992, p. 54). 
(6)EFSA Journal 2017;15(2):4739. (5)  According to the EFSA Assistance, quarantining salamanders, the testing of salamanders to demonstrate that they 
are free of infection from Bsal, restr icting mov ements of salamanders, hygienic procedures and biosecur ity 
measures or treating salamanders against Bsal are important risk mitig ation measures in preventing the spread of 
that disease. 
(6)  The EFSA Assistance also highlight ed many gaps and uncer tainties in the current state of knowledg e as regards 
Bsal. In particular , it concluded, that due to the complexity of the taxonomy as well as the lack of current 
evidence concer ning whic h species are susceptible to Bsal, rules at the level of the taxonomic order are likely to 
be both more effective and more feasible than species-specific rules. 
(7)  It is theref ore appropr iate to lay down animal health protection measures for intra-Union trade in consignments 
of salamanders and the introduction of such consignments into the Union on the taxonomic order of Caudata, in 
order to ensure that Bsal is not spread by intra-Un ion trade in those animals and the introduction of such 
animals into the Union. These measures should take into account the risk mitig ation measures refer red to in the 
EFSA Assistance and in particular provide for the appropr iate quarantine, diagnostic testing and treatment of 
salamanders as well as for certification of their health status for their trade in and introduction into the Union. 
These measures are of an emerge ncy nature and are not deemed specific measures within the meaning of 
Article 18(2) of Council Directive 92/65/EEC. 
(8)  Regulation (EU) No 576/2013 of the European Parliament and of the Council (1) lays down the animal health 
requirements applicable to the non-commercial move ments of pet animals liste d in Annex I thereto, and 
amph ibia are included in the animals listed in that Annex. The animal health prot ection measures laid down in 
this Decision should not apply to the non-commercial move ments of pet salamanders whic h fall within the scope 
of Regulation (EU) No 576/2013, due the specif icities of those movements and given the lack of information 
concer ning such mov ements. 
(9)  These measures should apply regar dless of other Union rules pote ntially relevant for trade in and introduction of 
salamanders, in particular Council Regulation (EC) No 338/97 (2). 
(10)  The World Org anisation for Animal Health (OIE) listed infec tion with Bsal in its Aquatic Animal Health Code 
during its 85th General Session on 21-26 May 2017. But detailed internati onal standards are not yet available 
and there is a lack of information on the technical capabilities of veterinar y services and laborat ories around the 
world to test for Bsal, while various concer ned stak eholders in the European Union are in the forefront of 
progress on the diagnosis and treatment of Bsal and the safe handling of traded salamanders. It is theref ore 
appropr iate that most risk-mitigating measures, in particular the quarantining in an appropr iate establishment, 
testing and treatment of traded and introduced salamanders are to be done by compet ent veterinar y author ities, 
operat ors and laboratori es locat ed within the Union. 
(11)  According to the EFSA Assistance, it is possible that Bsal is transmitte d between salamander species native to 
diffe rent areas and cross-contamination may take place in various establishments breeding, collecting or 
distr ibuting salamanders. This makes the risk of Bsal occur ring in traded salamanders independent from their 
place of origin and from the situation in the wild. Theref ore all consignments of salamanders destined for intra- 
Union trade or introduced into the Union should be subject to risk mitig ation measures. 
(12)  Minimum conditions for appropr iate establishments to be used for quarantining salamanders should be laid 
down to ensure their biosecur ity, while the handling of salamanders which die in those establishments should be 
done according to existing specifi c rules laid down in the Animal by-pro ducts Regulation (3). 
(13)  As regards the size of the quarantined epidemiological units, a minimum size eligible for certification for negative 
diagnostic test results should be specified due to the limite d sensitivity of the best available real-time quantitative 
polymerase chain reaction (qPCR) assa y which is suffi ciently reliable to be used only if the epidemiological units 
comprise at least 62 salamanders. 5.3.2018 L 62/19 Official Jour nal of the European Union EN     
(1)Regulation (EU) No 576/2013 of the European Parliament and of the Council of 12 June 2013 on the non-commercial movement of pet 
animals and repealing Regulation (EC) No 998/2003 (OJ L 178, 28.6.2013, p. 1). 
(2)Council Regulation (EC) No 338/97 of 9 December 1996 on the protection of species of wild fauna and flora by regulating trade therein 
(OJ L 61, 3.3.1997, p. 1). 
(3)Regulation (EC) No 1069/2009 of the European Parliament and of the Council of 21 October 2009 laying down health rules as regar ds 
animal by-products and derived products not inten ded for human consump tion and repealing Regulation (EC) No 1774/2002 (OJ L 300, 
14.11.2009, p. 1). (14)  Salamanders which have undergone quarantine with testing with negative results or satisfa ctor y treatment in the 
Union should not be subjecte d to quarantine or testing again, provid ed that they have been kept isolat ed from 
salamanders of a different health status in an appropr iate establishment. 
(15)  As regar ds treatments, these should be specifi ed and be in line with the protocols already descr ibed in peer - 
reviewed scientific literature as highlight ed by the EFSA Assistance or with comparable ones. 
(16)  A list of third countr ies approved for issuing animal health certificates for the introduction into the Union of 
consignments of salamanders should be established and limited to those countr ies which have already provid ed 
sufficient guarant ees for issuing certificates to prevent misleading and fraudulent certification , at least equivalent 
to Council Directive 96/93/EC (1). Theref ore, it is appropr iate to refer to already available lists existing in the 
cont ext of introduction into the Union of other commodities. Such third countr ies are listed in Annex I to 
Commission Decision 2004/211/EC (2), Part 2 of Annex II to Commission Decision 2007/777/EC (3), Annex I to 
Commission Regulation (EC) No 798/2008 (4), Part 1 of Annex I to Commission Regulation (EC) 
No 119/2009 (5), Part 1 of Annex II to Commission Regulation (EU) No 206/2010 (6), or Annex I to 
Commission Regulation (EU) No 605/2010 (7), respectively . 
(17)  Consignments of salamanders should be introduced in the Union only if they compl y with all the requirements 
and the compet ent veteri nary author ity of the border inspection post of entr y can also ascer tain that the 
consignments will be accept ed by an operat or responsible for an appropr iate establishment of destination to be 
duly quarantined. 
(18)  The actual arrival of consignments of salamanders introduced into the Union from third countr ies at their place 
of quarantine in the Union should be recorded in the electronic version of the common veterinar y entr y 
document set out in Annex I to Commission Regulation (EC) No 282/2004 (8) and manag ed by the integrat ed 
comput erised veterinar y system known as Traces, so that the compet ent veteri nary author ity of the border 
inspection post of entr y can be reliably informed of their arrival. 
(19)  This Decision should provide for a transitional period in order to give the Member States, the compet ent 
author ities and economic operators time to put in place the necessar y procedures so that they are in a position 
to compl y with the rules laid down in this Decision. The length of this period should be limite d to a few 
months. At the same time, risk mitig ation measures based on the required level of prot ection by the Member 
States of destination should already apply . 
(20)  It is expect ed that more information will be available in the coming years about Bsal from scientific sources and 
from results of offici al controls carried out by the Member State s, supplementing the current knowledg e 
concer ning that disease. Theref ore, the animal health protection measures laid down in this Decision should be of 
a temporar y nature. Never theless, they should apply at least until 31 December 2019 to allow for 1 year of 
implementation by the Member States and subsequent annual repor ting and consideration thereof, while 
permanent EU animal health rules may be laid down under the new Regulation (EU) 2016/429 on transmissible 
animal diseases to apply from the date of application of that Regulation. 5.3.2018 L 62/20 Official Jour nal of the European Union EN     
(1)Council Directive 96/93/EC of 17 December 1996 on the certification of animals and animal products, (OJ L 13, 16.1.1997, p. 28). 
(2)Commission Decision 2004/211/EC of 6 Januar y 2004 establishing the list of third countr ies and parts of territory thereof from which 
Member States author ise impor ts of live equidae and semen, ova and embr yos of the equine species, and amending Decisions 
93/195/EEC and 94/63/EC (OJ L 73, 11.3.2004, p. 1). 
(3)Commission Decision 2007/777/EC of 29 November 2007 laying down the animal and public health conditions and model certificates 
for impor ts of certain meat products and treated stomachs, bladders and intest ines for human consump tion from third countr ies and 
repealing Decision 2005/432/EC (OJ L 312, 30.11.2007, p. 49). 
(4)Commission Regulation (EC) No 798/2008 of 8 August 2008 laying down a list of third countr ies, territories, zones or compar tments 
from which poultr y and poultr y products may be impo rted into and transit through the Community and the veterinar y certification 
requirements (OJ L 226, 23.8.2008, p. 1). 
(5)Commission Regulation (EC) No 119/2009 of 9 Febr uary 2009 laying down a list of third countr ies or parts thereof, for impor ts into, or 
transit through, the Community of meat of wild lepor idae, of certain wild land mammals and of farmed rabbits and the veterinar y certifi­
cation requirements (OJ L 39, 10.2.2009, p. 12). 
(6)Commission Regulation (EU) No 206/2010 of 12 March 2010 laying down lists of third countr ies, territories or parts thereof author ised 
for the introduction into the European Union of certain animals and fresh meat and the veterin ary certification requirements (OJ L 73, 
20.3.2010, p. 1). 
(7)Commission Regulation (EU) No 605/2010 of 2 July 2010 laying down animal and public health and veterinar y certification conditions 
for the introduction into the European Union of raw milk, dair y products, colostr um and colostr um-based products intended for human 
consump tion (OJ L 175, 10.7.2010, p. 1). 
(8)Commission Regulation (EC) No 282/2004 of 18 Febr uary 2004 introducing a document for the declaration of, and veterinar y checks 
on, animals from third countr ies enteri ng the Community (OJ L 49, 19.2.2004, p. 11). (21)  The measures provided for in this Decision are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed, 
HAS ADOPTED THIS DECISION: 
Article 1 
Subject and scope 
This Decision lays down the animal health prote ction measures for intra-Union trade in consignments of salamanders 
and the introduction of such consignments into the Union. 
Article 2 
Def initions 
For the purposes of this Decision, the following definitions shall apply: 
(a)  ‘salamanders’ means all amphibians of the order Caudata; 
(b) ‘Bsal’ means the fungus Batr achochytr ium salamandr ivorans (kingdom fungi, Phylum Chytr idiomy cota, order Rhizophy ­
diales); 
(c)  ‘epidemiological unit ’ means a group of salamanders with the same likelihood of exposure to Bsal; 
(d)  ‘quarantine’ means the keeping of salamanders in isolation with no direct or indirect contact with salamanders 
outside their epidemiological unit, for the purposes of ensur ing that there is no spread of Bsal while the animals in 
isolation are undergoing obser vation for a specif ied length of time and testing and, if appropr iate, treatment ; 
(e)  ‘operator ’ means any natural or legal person having salamanders under its responsibility , including for a limited 
duration of time, but excluding pet keepers; 
(f)  ‘appropr iate establishment’ means premises: 
(i)  where salamanders are kept in quarantine before being dispatche d to another Member State or after their 
introduction into the Union; and 
(ii)  which are registered by the compet ent author ity prior to the date of commencement of any quarantine; 
(g)  ‘appropr iate diagnostic test’ means a real-time quantitative polymerase chain reaction (qPCR) assa y containing 
species-specific STerF and STerR primers ampli fying a 119 nucleotide long fragment of Bsal DNA; 
(h)  ‘common veteri nary entr y document’ or ‘CVED’ means the document notifying the arrival of animals into the Union 
as provid ed for in Article 1 of Regulation (EC) No 282/2004 and drawn up in accordance with the model set out in 
Annex I thereto and managed by the integrated comput erised veteri nary system kno wn as Traces; 
(i)  ‘confi rmed case of Bsal’ means the conf irmation of the presence of Bsal or its genetic mate rial on or in the tissues of 
salamanders by the appropr iate diagnostic test; 
Article 3 
Animal health conditions for intra-Union trade in salamanders 
1. Member State s shall prohibit the dispatch of consignments of salamanders to another Member State, excep t where 
such consignments compl y with the followi ng animal health conditions: 
(a)  they are accompan ied by an animal health certificate which complies with the model animal health certificate set 
out in Part A of Annex I; 5.3.2018 L 62/21 Official Jour nal of the European Union EN     (b)  the salamanders must not show clinical signs of Bsal, in particular they must show no skin lesions and ulcers at the 
time of examination by the official veterinar ian; that examination must be carried out within a period of 24 hours 
prior to the time of dispatch of the consignment to the Member State of destination; 
(c)  the salamanders must come from a population where there have been no mor talities due to Bsal and no clinical 
signs of Bsal, in particular skin lesions and ulcers must not have been obser ved by the operator; 
(d)  the consignment must consist of: 
(i)  at least 62 salamanders which have undergone quarantine as one epidemiological unit in an appropr iate 
establishment which complies with the minimum conditions set out in Annex II for a period of at least 6 weeks 
immediately prior to the date of the issuing of the animal health certificate set out in Part A of Annex I and skin 
swab samp les from the salamanders in the consignment must have been tested for Bsal with nega tive results 
during the fifth week of the period of quarantine with the appropr iate diagnostic test, in accordance with the 
sample sizes set out in point 1(a) of Annex III; or 
(ii)  salamanders which have been treated to the satisfaction of the compe tent author ity against Bsal in accordance 
with point 1(b) of Annex III. 
2. Where consignments of salamanders have been introduced into the Union from a third countr y and they have 
already undergone quarantine in an appropr iate establishment of destination in accordance with Article 6, Member 
States shall only author ise their dispatch to another Member State where such consignments compl y with the follo wing 
conditions: 
(a)  the animal health conditions laid down in paragraph 1(a), (b) and (c); 
(b)  the salamanders have been kept in quarantine in the appropr iate establishment whic h complies with the minimum 
conditions set out in Annex II, between the end of the period of quarantine followi ng their introduction into the 
Union and the issuing of the animal health certificate set out in Part A of Annex I. 
Article 4 
Animal health conditions for the introduction into the Union of consignments of salamanders 
Member State s shall prohibit the introduction into the Union of consignments of salamanders from a third countr y, 
excep t where such consignments compl y with the following conditions: 
(a)  they come from third countr ies listed in one of the followi ng: 
(i)  Annex I to Decision 2004/211/EC; 
(ii)  Part 2 of Annex II to Decision 2007/777/EC; 
(iii)  Annex I to Regulation (EC) No 798/2008; 
(iv)  Part 1 of Annex I to Regulation (EC) No 119/2009; 
(v)  Part 1 of Annex II to Regulation (EU) No 206/2010; 
or 
(vi)  Annex I to Regulation (EU) No 605/2010; 
(b)  they are accompan ied by an animal health certificate which complies with the model animal health certificate set 
out in Part B of Annex I; 
(c)  the salamanders must not show clinical signs of Bsal, in particular there must be no signs of skin lesions and ulcers 
at the time of examination by the official veteri narian; and that examination must have been carried out within 
a period of 24 hours prior to the time of dispatch of the consignment to the Union; 5.3.2018 L 62/22 Official Jour nal of the European Union EN     (d)  before issuing the animal health certificate refer red to in point (b), the epidemiological unit compris ing the 
salamanders in the consignment must have been isolated from other salamanders at the lates t at the time of the 
examination for the purposes of issuing of animal health certificate and they must not have been in contact with 
other salamanders since that time. 
Article 5 
Attes tation regarding the appropr iate establishment of destination 
Member States shall ensure that border inspection posts do not accept the entr y into the Union of consignments of 
salamanders unless importers or their agents provide a written attestation, in an official language of the Member State of 
the border inspection post of entr y into the Union, which is signed by the natural or lega l person responsible for the 
appropr iate establishment of destination, stating: 
(a)  the name and address of the appropr iate establishment of destination; 
(b)  that the appropr iate establishment of destination compl ies with the minimum conditions set out in Annex II; 
(c)  that the consignment of salamanders will be accep ted for quarantine. 
Article 6 
Quarantine rules for consignments of salamanders introduced into the Union 
Member States shall ensure that:  
1. The official or appro ved vete rinar ian responsible for the appropr iate establishment of destination records the arrival 
of the consignment of salamanders introduced into the Union from a third countr y in Box 45 of Part 3 in the 
electronic version of the common veteri nary entr y document.  
2. The offici al or approved veter inar ian ensures that the operator keeps the consignment of salamanders in quarantine 
in the appropr iate establishment of destination as one epidemiological unit.  
3. The official or approved veteri narian inspects the conditions of quarantine for each consignment of salamanders, 
including an examination of the mor tality records and a clinical inspection of the salamanders in the appropr iate 
establishment of destination, chec king in particular for skin lesions and ulcers.  
4. Where a consignment compris es 62 or more salamanders, the official or approved vete rinar ian carries out the 
examination, sampling, testing and treatment procedures for Bsal in accordance with the procedures refer red to in 
points 1 and 2 of Annex III, follo wing the arrival of the consignment of salamanders at the appropr iate establishment 
of destination.  
5. Where the consignment compr ises less than 62 salamanders, the official or approved veteri narian ensures that the 
consignment is treated to the satisfaction of the compet ent author ity against Bsal in accordance with point 3 of 
Annex III.  
6. The official or approved veteri narian releases the consignment of salamanders from the appropr iate establishment of 
destination by a written author isation: 
(a)  in case of testing as referred to in point 1(a) of Annex III, provided that at least 6 weeks has elapsed since the 
date of the the commencement of the period of quarantine and not before the receipt of the negativ e test results, 
whic hever is later; or 
(b)  in the case of treatment as referred to in point 1(b) of Annex III, only after satisf actory completion of the 
treatment. 5.3.2018 L 62/23 Official Jour nal of the European Union EN     Article 7 
Measures to be taken in the event of a conf irmed case of Bsal in an appropr iate establishment of 
destination 
1. Member States shall ensure that where during quarantine it is confir med that at least one salamander of an epi­
demiological unit is infect ed with Bsal, the followi ng measures are take n by the appropr iate establishment of destination: 
(a)  all salamanders in the same epidemiological unit are either: 
(i)  treated to the satisf action of the compe tent author ity against Bsal in accordance with point 3 of Annex III; or 
(ii)  killed and disposed of as animal by-pro ducts in accordance with Article 12 of Regulation (EC) No 1069/2009. 
(b)  followi ng compl etion of the measures refer red to in point (a), the area of the appropr iate establishment of 
destination where the epidemiological unit had been kept is cleaned and disinfect ed to the satisf action of the 
compet ent author ity. 
2. The compet ent author ity may require testing of the treated salamanders to verify the effectiveness of the treatment 
referred to in point 1(a)(i) and may require repeate d treatments, as appropr iate, to prevent the spread of Bsal. 
Article 8 
Cost s 
Member State s shall ensure that all quarantine costs, testing costs and, where necessar y, the costs of risk mitigation 
measures and treatments are borne by the operat or or the impor ter. 
Article 9 
Annual repor ting requirements 
At the lates t by 30 June each year , starting from 2019, those Member State s which have handled salamander 
consignments in the previous year, shall submit to the Commission the follo wing information concer ning the previous 
year, differentiating between information relat ed to the intra-Un ion trade or to introductions of salamander 
consignments into the Union: 
(a)  the number of epidemiological units with at least one conf irmed case of Bsal; 
(b)  the number of epidemiological units treated without a conf irmed case; 
(c) any further information they deem relevant on testing, treating or handling the consignments and on the implemen ­
tation of this Decision. 
Article 10 
Transitional measures 
1. For a transitional period until 6 Sept ember 2018, the Member States of destination may accept on their territory 
consignments of salamanders from other Member State s which do not compl y with the animal health conditions laid 
down in Article 3, under appropr iate risk mitig ation conditions to be determined by the compet ent author ity after 
consulting the operat ors and, if necessar y, the Member State of origin. 
2. For a transitional period until 6 Sept ember 2018, the Member States of destination may accept on their territory 
consignments of salamanders introduced into the Union from a third countr y which do not compl y with the animal 
health conditions laid down in Article 4, provid ed that they are handled in accordance with Articles 5 to 7. 5.3.2018 L 62/24 Official Jour nal of the European Union EN     Article 11 
Applicability 
This Decision shall apply until 31 December 2019. 
Article 12 
Addre ssees 
This Decision is addressed to the Member States. 
Done at Brussels, 28 Febr uary 2018. 
For the Commission 
Vytenis ANDRIUKAITIS 
Member of the Commission  5.3.2018 L 62/25 Official Jour nal of the European Union EN     ANNEX I 
PART A 
ANIMAL HEAL TH CERT IFIC ATE 
for intra-Union trade in salamanders 
5.3.2018 L 62/26 Official Jour nal of the European Union EN     5.3.2018 L 62/27 Official Jour nal of the European Union EN     5.3.2018 L 62/28 Official Jour nal of the European Union EN     PART B 
ANIMAL HEAL TH CERT IFIC ATE 
for the introduction of consignments of salamanders into the European Union 
5.3.2018 L 62/29 Official Jour nal of the European Union EN     5.3.2018 L 62/30 Official Jour nal of the European Union EN     5.3.2018 L 62/31 Official Jour nal of the European Union EN     ANNEX II 
MINIMUM CONDITIONS FOR APPR OPRIA TE EST ABLISHMENTS OF DESTINA TION 
(1)  The appropr iate establishment of destination: 
(a)  has a syste m that ensures adequate surveillance of the salamanders; 
(b)  is under the control of an official or appro ved veterinar ian; 
(c)  is cleaned and disinf ected in accordance with instr uctions by the compet ent author ity. 
(2)  The operat or of the appropr iate establishment ensures that : 
(a)  cleaning and disinfection of the tank s, crates or other fomites used for the transpor t of the salamanders is 
carried out unless those are destro yed, in a way to prevent the spread of Bsal. 
(b)  wast e mate rial and waste wate r is collect ed regularly , store d and subsequently treated in a way to prevent the 
spread of Bsal. 
(c)  carcases of quarantined salamanders are examined in a laboratory indicated by the compet ent author ity. 
(d)  the necessar y tests and treatments of salamanders are carried out in consultation with and under the control of 
the official or approved veterinar ian. 
(3)  The operat or of the appropr iate establishments of destination informs the official or approved veteri narian of 
diseases and deaths of salamanders during the quarantine. 
(4)  The operat or of the appropr iate establishments of destination keeps a record of: 
(a)  the date, number and species of salamanders entering and leav ing for each consignment ; 
(b)  copies of the animal health certificates and the common veterinar y entr y documents accompan ying the 
consignment of salamanders; 
(c)  cases of illness and the number of deaths on a daily basis; 
(d)  dates and results of testing; 
(e)  types and dates of treatment and the number of animals subjecte d to it.  5.3.2018 L 62/32 Official Jour nal of the European Union EN     ANNEX III 
EXAMINA TION , SAMPLIN G, TESTING AND TREA TMENT PROCEDURES FOR BSAL 
(1)  Dur ing quarantine, the salamanders are subjected to the followi ng procedures: 
(a)  If the size of the epidemiological unit is 62 or more, skin swab samples from quarantined salamanders must be 
examined under the control of the official or approved veteri narian with the appropr iate diagnostic test during 
the fifth week following the date of their entr y into the appropr iate establishment, in accordance with the 
sam ple sizes set out in the reference table, unless the operator opts for treatment in accordance with point (b). 
Reference Table (1): 
Size of the epidemiological unit  62  186  200  250  300  350  400  450 
Sam ple size  62  96  98  102  106  108  110  111 
(1)  Assuming 3 % prevalence of Bsal in the epidemiological unit and ensur ing its detection with a 95 % confidence rate with 
the sensitivity of the appropr iate diagnostic test calculated to be at 80 %.  
(b) If the operat or opts for one of the treatments listed in point (3) or in all cases where the size of the epidemiol ­
ogical unit is less than 62, all salamanders in the consignment must be treated by the operat or against Bsal 
under the control of the official or approved veteri narian to the satisf action of the compe tent author ity. 
(c) In cases referred to in point (b) the official or approved veteri narian may require representative testing of the epi­
demiological unit with the appropr iate diagnostic test before the treatment to monit or the presence of Bsal or 
after treatment to verify the absence of Bsal. 
(d)  Skin swab samples from all dead or clinically sick salamanders, in particular those with skin lesions, must be 
examined under the control of the official or approved veteri narian with the appropr iate diagnostic test at the 
time they show lesions or other clinical signs or at the time of death, which ever is sooner . 
(e)  All salamanders whic h die in the appropr iate establishment must be subjected to a post mor tem examination 
under the control of the official or approved veterinar ian, in particular to check for signs of Bsal, to confir m or 
to excl ude Bsal as cause of death, to the extent possible. 
(2)  All testin g of samples taken and post mor tem examination during quarantine must be carried out in laboratories 
indicated by the official or approved veteri narian. 
(3)  The followi ng treatments are considered to be satisfact ory: 
(a)  keeping salamanders at a temperature of at least 25 °C for at least 12 days; 
(b)  keeping salamanders at a temperature of at least 20 °C for at least 10 days combined with a treatment with 
polym yxin E submersion baths (2 000 IU/ml) for 10 minutes twice per day, followed by applying vori conazole 
spra y (12,5 μg/ml); 
(c)  any other treatment with comparable results in eliminating Bsal as repor ted in a peer -reviewed article published 
in a scientific jour nal.  5.3.2018 L 62/33 Official Jour nal of the European Union EN