Reference number  
DRS  383: 2018  
© RSB 2018  
 
 
 RWANDA 
STANDARD  DRS 
383 
 
First edition  
2018 -mm-dd 
 
Antiseptics based on chlorhexidine 
gluconate — Specification  
 
  DRS  383: 2018  
©RSB 2018 - All rights reserved                  ii 
 In order to match with technological development and to keep continuous progress in industries, 
standards are subject to periodic review. Users shall ascertain that they are in possession of the latest 
edition  
 
 
 
 
 
 
 
 
© RSB 2018  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized 
in any form or by any means, electronic or mechanical, including photocopying and microfilm, without 
prior written permission from R SB. 
Requests for permission to reprodu ce this document should be addressed to : 
Rwanda Standards  Board  
P.O Box 7099 Kigali -Rwanda  
KK 15 Rd, 49  
Tel. +250 788303492  
Toll Free: 3250  
E-mail: info@rsb.gov.rw  
Website: www.rsb.gov.rw  
ePortal: www.portal.rsb.gov.rw  DRS  383: 2018  
iii                ©RSB 2018 - All rights reserved  
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ...................  iv 
1 Scope  ................................ ................................ ................................ ................................ .............  1 
2 Normative references  ................................ ................................ ................................ ...................  1 
3 Term s and definitions  ................................ ................................ ................................ ..................  1 
4 Requirements  ................................ ................................ ................................ ................................  2 
4.1 General requirements  ................................ ................................ ................................ ..................  2 
4.2 Specific requirements  ................................ ................................ ................................ ..................  2 
5 Packaging and labelling ................................ ................................ ................................ ...............  3 
5.1 Packaging  ................................ ................................ ................................ ................................ ...... 3 
5.2 Labelling  ................................ ................................ ................................ ................................ ........  3 
6 Inspection  ................................ ................................ ................................ ................................ ...... 4 
Annex  A (normati ve)  Bactericidal efficacy  ................................ ................................ ............................  5 
A.1 Apparatus  ................................ ................................ ................................ ................................ ...... 5 
A.2 Accuracy  ................................ ................................ ................................ ................................ ....... 5 
A.3 Test media  ................................ ................................ ................................ ................................ ..... 5 
A.4 Reference cultures  ................................ ................................ ................................ .......................  7 
A.5 Test procedure  ................................ ................................ ................................ ..............................  7 
A.6 Interpretation of results  ................................ ................................ ................................ ...............  8 
Annex  B (normative)  Determination of matter insoluble in water  ................................ .....................  10 
B.1 Standard hard water  ................................ ................................ ................................ ...................  10 
B.2 Procedure  ................................ ................................ ................................ ................................ .... 10 
B.3 Calculation  ................................ ................................ ................................ ................................ .. 10 
Annex  C (nor mative)  Determination of corrosiveness to aluminium  ................................ ...............  12 
C.1 Principle  ................................ ................................ ................................ ................................ ...... 12 
C.2 Apparatus and reagents  ................................ ................................ ................................ ............  12 
C.3 Corrosiveness to steel  ................................ ................................ ................................ ...............  12 
C.4 Procedure  ................................ ................................ ................................ ................................ .... 12 
C.5 Calculation  ................................ ................................ ................................ ................................ .. 13 
C.6 Corrosiveness to aluminium  ................................ ................................ ................................ ..... 13 
Annex  D (normative)  Consistency and storage stability  ................................ ................................ .... 14 
Annex  E (normative)  Determination of foaming properties  ................................ ...............................  15 
 DRS  383: 2018  
©RSB 2018 - All rights reserved                  iv 
 Foreword  
Rwanda Standard s are prepared  by Technical Committees and approved by R wanda Standards Board (RSB) 
Board  of Directors in  accordance with the procedures of RSB, in compliance with Annex 3 of the WTO/TBT 
agreement on the preparation, adoption and application of standards.  
The main task of technical committees is to prepare national standards.  Final Draft Rwanda S tandards adopted 
by Technical committ ees are ratifie d by me mbers of RSB Board of Director s for publication and gazettment as 
Rwanda Standards.  
DRS  383 was prepared by Technical Committee RSB/TC 042, Surface active Agents . 
In the preparation of this standard, reference was made to the following standard:  
SANS 1597, Antiseptics based on chlorhexidine gluconate  
The assistance derived from the above source is hereby acknowledged with thanks.  
Committee membership  
The following organizations were represented on the Technical Committee on Surface Active agents ( RSB/TC 
042) in the preparation of this standard.  
Paragraph of participants  
University of Rwanda – College of Sciences and Technology  
Rwanda Biomedical Center – Medical Procurement and Production Division  
Trust Industries Limited  
SULFO Rwanda Industries  
Private Sector Federation – Beauty Makers Association  
Mount Kenya University  
IKIZERE Initiative Hope Shines  
Rwanda  Standards Board (RSB) – Secretariat  
  DRS  383: 2018  
1                ©RSB 2018 - All rights reserved  
 Antiseptic based on chlorhexidine gluconate  — Specification  
1 Scope  
This Draft Rwanda Standard specifies requirements , sampling, test method s and inspection  for antiseptic 
products based on chlorhexidine gluconate that may contain detergent  (s) and other compatible chemical 
agents, and that are water -miscible and intended for use on live tissue that is free from excessive dirt.  
This document does not cover th e antiseptic products with a chlorhexidine gluconate content of less than 0.5% 
by volume . 
2 Normative references  
The following documents are referred to in the text in such a way that some or all of  their content constitutes 
requirements of this document.  For dated references, only the edition  cited applies. For undated references, the 
latest edition of the referenced document (including any amendments) applies.  
RS 278, Cosmetics – Method of Sampling  
RS ISO 862, Surface active agents – Vocabulary  
ISO 7218, Microbiology of food and animal feeding stuffs -- General requirements and guidance for 
microbiological examinations  
ISO 10523, Water quality -- Determination of pH  
RS EAS 346, Labelling of cosmetic products — General requirements  
ISO 11133, Microbiology of  food, animal feed and water -- Preparation, production, storage and performance 
testing of culture media  
3 Terms and definitions  
For the purposes of this standard, the terms and definitions given in RS ISO 862  and the following apply.  
3.1 
chlorhexidine gluconate  
antibacterial compound C 22H30Cl2N10 used as a local antiseptic and disinfectant especially in the form of its 
hydrochloride, gluconate, or acetate  
  
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 3.2 
antiseptic  
chemical agent without added detergent(s) that kills most vegetative forms of patho genic and other micro -
organisms (but not necessarily all resistant micro -organisms) and that is suitable, when used as directed , for 
application to live tissue . 
3.3 
antiseptic skin cleanser  
antiseptic that contains a detergent(s) and that cleans lightly soiled skin surfaces, kills most vegetative forms of 
pathogenic and other micro -organisms (but not necessarily all resistant microorganisms) and that is suitable, 
when used as directed, for application to live tissue.  
3.4 
batch  
that quantity of sealed cont ainers of antiseptic that have been filled from one homogeneous blend or, in the 
case of a continuous production process, that have been filled from one day's production.  
4 Requirements  
4.1 General requirements  
4.1.1  The product shall be a clear or slightly opal escent, viscous and homogeneous water -miscible liquid 
and shall be one of the following types : 
Type 1: an antiseptic skin cleanser; or  
Type 2: an antiseptic without added detergents.  
4.1.2  The product  shall not be irritating to the normal skin and shall no t contain any ingredients in a quantity 
that is toxic to human beings.  
4.1.3 During storage at ambient temperature, the odour of the product  shall remain such as to be acceptable, 
and when perfumed, the fragrance shall not change.  
4.1.4  The product  shall, remain stable up to its best before date in the original container under normal storage 
conditions, still comply with all the requirements of this standard.  
4.2 Specific requirements  
4.2.1  When the product  is tested in accordance with Annex  A, it shall , within 1 min, obtain a log reduction of 
at least three (3 log 10) of the organisms listed in A.4.1,  in cases where:  DRS  383: 2018  
3                ©RSB 2018 - All rights reserved  
 a) the supplied product  contains 5 % chlorhexidine gluconate or more; or  
b) the supplied product  contains less than 5 % chlorhexidine gluconate, at the prescribed concentration.  
4.2.2  The product shall also comply with the requirements given in Table 1, whe n tested in accordance with 
the methods described therein.  
Table 1 – Specific requirements  
S/N Parameters  Requirements  Test methods  
(i)  Water inso luble matter, g/l, max  2 Annex B  
(ii)  Corrosiveness to aluminium  pass the test  Annex C 
(iii)  pH 5.5 – 6.5 RS ISO 10523  
(iv)  Total plate count, cfu/ml, max.  10 RS ISO 7218  
(v)  Foaming properties  Pass test  Annex E  
4.2.3  Consistency and storage stability  
4.2.3.1 In accordance to Annex D , the product  shall remain homogeneous and free -flowing. After standing at a 
temperature of 20 °C ± 5 °C for a further 24 h, the antiseptic shall show no sign of precipitation or separation.  
4.2.3.2 The product  shall show no sign of pr ecipitation or separation and shall comply with all the relevant 
requirements of this standard.  
5 Packaging and labelling  
5.1 Packaging  
5.1.1 The containers (and their closures) in which the product  is pack aged shall not interact chemically or 
physically with the antiseptic. They shall be of an acceptable design and shall be strong enough to protect the 
antiseptic during normal handling, transportation and storage.  
 
5.1.2 The closure shall not be made of cork or of any material that contains cork.  
 
5.1.3 Only c ontainers of the same size and bearing the same batch identification shall be packed together in a 
bulk container.  
5.2 Labelling  
In addition to the labeling requirement in accordance with RS EAS 346 , the following information shall appear 
in legible and indeli ble marking on each container or on a label securely attached to each container (if 
containers are too small to accommodate the instructions regarding dilution rate) : 
 
a) the manufacturer's name and trade name or trademark;  
b) a statement that the product is an antiseptic or an antiseptic skin cleanser (whichever is  applicable) 
based on chlorhexidine gluconate;  
c) the type of antiseptic (see 4.1);  
d) the concentration, as a percentage of the total volume, of the chlorhexidine gluconate content of  the 
antiseptic skin cl eanser;  
e) the nominal volume of the contents, in plain type and in a colour that contrasts distinctly with that  of 
the container or label;  
f) the batch identification or the production date (or both);  
g) the expiry date of the batch;   
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 h) general instructions for use, including the prescribed concentration (the dilution rates), for the  various 
purposes for which the antiseptic is suitable;  
i) the pH value at the prescribed concentration (see 6.2 (h)); and  
j) the following warnings:  
1 unless recommended by the manufacturer, do n ot mix the antiseptic with other substances;  and 
2 the antiseptic is sensitive to temperature and light and should be stored in closed containers in a 
dry place at a temperature of 23 °C ± 2 °C/50 %RH, and protected from intense light.  
 
6 Inspection  
Visually, or otherwise, inspect each container, taken in accordance with RS 278 for compliance with the 
requirements of the standard . 
 DRS  383: 2018  
5                ©RSB 2018 - All rights reserved  
 Annex  A 
(normative)  
 
Bactericidal efficacy  
A.1 Apparatus  
The apparatus and equipment shall comply with the requirements given in ISO  7218.  
A.2 Accuracy  
Except where otherwise specified, allow the following tolerances:  
a) on temperatures...................................... ± 2 °C  
b) on masses............................................... ± 1,0 %  
c) on volumes.............................................. ± 1,0 %  
d) on pH value............................................. ± 0,1 pH units  
A.3 Test media  
A.3.1  General  
Media and reagents shall comply with the requirements given in ISO 7218 and ISO 11133. Many of  the media 
required are obtainable commercially in dehydrated form and, for uniformity of  results, the use of such media 
is recommended. If these are used, follow the manufacturer's instructions strictly regarding the reconstitution 
and sterilization. The water used in the preparation of media shall be distilled water.  
 
A.3.2 Tryptone soy broth  
 
A.3.2.1 Ingredients  
 
Tryptone .................................................................. 17,0 g  
Sodium chloride....................................................... 5,0 g  
Soy peptone ............................................................ 3,0 g  
Potassium hydrogen dibasic phosphate.................. 2,5 g  
Dextrose ..................................... ............................. 2,5 g  
Water ....................................................................... 1 000 mL  
 
NOTE  Nutrient broth may be used instead of tryptone soy broth.  
A.3.2.2 Preparation  
Dissolve the ingredients in water. Heat, if neces sary, to ensure that the ingredients are completely dissolved. 
Adjust the pH value to 7.3. Dispense 10 mL volumes into tubes and sterilize by autoclaving at (1200+3) °C for 
15 min.  
  
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 A.3.3 Nutrient agar  
 
A.3.3.1 Ingredients  
 
Agar ..................................... 15,0 g  
Peptone ............................... 5,0 g  
Sodium chloride................... 5,0 g  
Yeast extract........................ 2,0 g  
Beef extract ......................... 1,0 g  
Water ................................... 1 000 mL  
 
 
A.3.3.2 Preparation  
 
Dissolve the ingredients in the water and bring to boil. Adjust the pH value to 7.1. Dispense 10 mL and 15 mL 
volumes into suitable bottles and sterilize by autoclaving at (1200+3)°C for 1 5 min. Allow the 10 mL volumes to 
solidify in a sloped position.  
 
A.3.4  Neutralizer media  
 
A.3.4.1 tryptone soy azolecitn inactivator  
 
A.3.4.1.1 Ingredients  
 
Tryptone soy broth (see 5.2.3.2) ........................... 100 mL  
Soy lecithin (azolectin)........................................... 3.0 g  
Polyoxyethylene sorbitan mono -oleate ................. 20 g  
 
A.3.4.1.2 Preparation  
 
Boil the ingredients with constant stirring until the ingredients are completely dissolved. Cool down to room 
temperature. Adjust the pH value to between 7.0 and 7.4. Dispense 9 mL volumes into suitable bottles and 
sterilize by autoclaving at (1200+3)°C for 15 min.  
 
NOTE  Sometimes the azolectin/polyoxyethylene emulsion can settle to the bottom of the container afte r sterilization. If 
this happens, storage of approximately one week at room temperature usually allows the solids to redissolve. If the 
neutralizer is required sooner, heating in a water  bath at (450+2)°C followed by occasional swirling during cooling will  
redissolve the solids.  
 
A.3.4.2 Cetrimide inactivator  
A.3.4.2.1 Ingredients  
Polyoxyethylene sorbitan mono -oleate ................... 8,0 g  
Sodium taurocholate................................................ 8,0 g  
Sodium thiosulfate ................................................... 1,5 g  
Potassium phosphate monobasic ........................... 0,5 g  
Sodium citrate.......................................................... 0,5 g  
Water ................................................. ...................... 1 000 mL  
 
A.3.4.2.2 Preparation  
 
Dissolve the ingredients in the water by heating. Dispense 20 mL volumes into suitable bottles and sterilize by 
autoclaving at (1200+3)°C for 15 min.  DRS  383: 2018  
7                ©RSB 2018 - All rights reserved  
  
A.4 Reference cultures  
 
A.4.1 Test organisms  
 
Use the  following test organisms:  
 
a) Pseudomonas aeruginosa : Pse 16, ATCC 15442; and  
b) Staphylococcus aureus : Sta 10, ATCC 6538.  
 
A.4.2 Maintenance of test organisms  
 
A.4.2.1 From a newly opened freeze -dried culture, or recently received agar culture, subculture each test 
organism into tubes of tryptone soy broth (see A.3.2).  
 
A.4.2.2 Incubate the tubes at 37 °C for 24 h. Subculture from the tubes onto nutrient agar (see A.3.3) for each 
organism. Incubate the slopes at 37 °C for 24 h.  
 
A.4.2.3 From each  of these slope cultures, prepare four subcultures (stock cultures) of each test organism 
onto 10 mL nutrient agar slopes (see A.3.3). Incubate the stock cultures at 37 °C for 24 h and then store them 
in a refrigerator at 4 °C, except for Pseudomonas aerug inosa , which is stored at ambient temperature.  
 
A.4.3 Preparation of the reference cultures for test suspensions  
 
A.4.3.1 From a stock culture (see A.4.2.3), inoculate each of the test organisms into tubes of tryptone soy 
broth (see A.3.2) and incubate at 37 °C for 24 h.  
 
A.4.3.2 For the test, use a 24 h culture that has been subcultured for two successive days.  
 
A.4.3.3 Use a haemocytometer, Petroff -Häusser counting chamber or any other suitable means to ensure that 
the concentration of the test organisms is 1.0 × 107 to 1.0 × 108 organisms per millilitre.  
 
A.5 Test procedure  
 
NOTE  Before the test for bactericidal efficacy is carried out, it is recommended that the efficacy of the inactivator be 
checked to ensure that the antiseptic to be tested is effectively neutralized.  
 
A.5.1 Using sterile distilled water, prepare a dilution in accordance with the instructions on the label,  where 
relevant.  
 
A.5.2 Dispense 9 mL volumes of the sample aseptically into two sterile test tubes and place them in a water  
bath maintained at 37 °C for 10 min.  
 
A.5.3 Repeat the procedure in A.5.2, but use 9 mL of sterile distilled water for the control . 
 
A.5.4 Add 1 mL of the Staphylococcus aureus suspension (see A.4.3.3) to one of the tubes (see A.5.2) and 
start a stopwatch immediately.  
 
A.5.5 Using a vortex, mix the contents of the tube and replace it in the water  bath.  
 
A.5.6 Repeat the procedure in A.5.4 to A.5.5 (inclusive) for the control (see A.5.3).   
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A.5.7 Using a clean sterile pipette, draw up 1 mL of the mixture from the tube (see A.5.5). At exactly 1 min 
exposure time, expel the mixture into a 9 mL inactivator medium (see A.3.4.1).  
 
A.5.8 Using a vortex, mix well. Allow the mixture to stand for at least 10 min before continuing with the test 
procedure.  
 
A.5.9 Using sterile distilled water as diluent, prepare tenfold dilutions of the mixture in A.5.8.  
 
A.5.10 Using a sterile pipette, dispens e 1 mL of each dilution in A.5.9 into a labelled Petri dish. Add 15 mL of 
cooled nutrient agar (see A.3.3) to each Petri dish.  
 
A.5.11 Mix thoroughly and allow the agar to solidify.  
 
A.5.12 Repeat the procedure in A.5.7 to A.5.11 (inclusive) for the control (see A.5.3).  
 
A.5.13 Invert the plates and incubate at 37 °C for at least 36 h.  
 
A.5.14 After incubation, examine the plates for growth (the sample plates and the control plates). Count and 
record all the colonies on each plate that contains betwee n 30 and 300 colonies. Ensure that the colonies that 
have been counted are derived from survivors of the test organisms and not from contamination.  
 
A.5.15 Take the dilution factor ( DF) for the sample and for the control as the inverse of the dilution, for  
example, if the sample has been diluted to 1:1 000, take DF as 1 000.  
 
A.5.16 The log reduction ( Rl) shall be determined using the following formula:  
 
  RI = log B – log A  
 
Where,  
 
RI  is the log reduction;  
 
log B  is the number of organisms counted in the  control (see A.5.14); and  
 
log A  is the number of organisms counted in the sample (see A.5.14).  
 
NOTE  Results may also be given in percentage kill.  
A.5.17 Repeat the procedure described in A.5.1 to A.5.16 (inclusive) using Pseudomonas aeruginosa as the 
test organism.  
 
A.5.18 Repeat the whole test described in A.5.1 to A.5.16 (inclusive) once more, but not on the same day.  
 
A.6 Interpretation of results  
 
A.6.1 Deem the sample to comply with the requirements of 4.2 if, for each organism tested, a 3 log 10 reduction 
is obtained.  
 
A.6.2 If there is a discrepancy in results between the two tests (see A.5.16 and A.5.18), repeat the whole test 
once more.  
 DRS  383: 2018  
9                ©RSB 2018 - All rights reserved  
 A.6.3 If the test in A.5.16 shows a 3 log 10 reduction, and if one of the results from A.5.18 or A.6.2 also s hows a 
3 log 10 reduction, the sample shall be deemed to have passed.    
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 Annex  B 
(normative)  
 
Determination of matter insoluble in water  
B.1 Standard hard water  
B.1.1  Ingredients  
Calcium chloride (CaCl 2.2H 2O) …………………………… 0.880 g  
Magnesium sulphate (MgSO 4.7H 2O) …………………….. 0.986 g  
Water ………………………………………………………… 5,000 mL  
B.1.2  Preparation  
Dissolve the calcium chloride and magnesium sulfate in distilled water and dilute to 5 L.  
B.2 Procedure  
B.2.1  Pipette 5.0 mL of the sample into a beaker and add 250 mL of the standard hard water (see B.1).  
 
B.2.2 Heat in a steam bath with frequent stirring until the sample is completely dispersed.  
 
B.2.3 Filter the solution immediately, under suction, through a tared 1.6 μm glass fibre filter and ensure that 
the insoluble matter is quantitatively transferred to the filter.  
 
B.2.4 Wash the beaker and the residue five times with 20 mL volumes of hot standard hard water (see B.1). 
Wash the filter with distilled water to remove salts from the hard water.  
 
B.2.5 Allow the solution to drai n completely and dry the residue at 105 °C ± 5 °C until constant mass is 
attained. Cool in a desiccator and weigh.  
 
B.3 Calculation  
 
Calculate the water -insoluble matter content ( S) in the test solution, expressed in grams per litre, using the 
following formul a: 
 
  S (g/l)= 200 M (g)  
 
Where,  
 
 S is the water -insoluble matter content, in grams per litre; and  
 DRS  383: 2018  
11                ©RSB 2018 - All rights reserved  
  M is the mass of the residue after it has been dried, in grams.   
©RSB 2018 - All rights reserved                  12 
 Annex  C 
(normative)  
 
Determination of corrosiveness to aluminium  
C.1 Principle  
A test strip is completely immersed in a detergent or a cleaning agent of a specified concentration. After 
conditioning the immersed test strip for a specified time under controlled temperature, the severity of the 
resulting corrosion is assessed visually for signs of pit ting and lost lustre, or by determining the loss in mass of 
the test strip per exposed surface area.  
C.2 Apparatus and reagents  
C.2.1 Apparatus  
 
C.2.1.1 Normally available laboratory glassware.  
 
C.2.1.2 Laboratory oven, maintained at temperature of 105 ºC ± 5 ºC 
 
C.2.2 Apparatus and reagents  
 
C.2.2.1 Ethanol  
 
C.2.2.2 Acetone  
C.3 Corrosiveness to steel  
C.3.1 Test strip  
 
One strip, of size approximately 80 mm x 10 mm x 1 mm, steel of a grade specified in the relevant national 
standard.  
C.4 Procedure  
C.4.1 Accurately determine the total area of the surfaces of the steel test strip and degrease it by  washing in a 
mixture of equal volumes of ethanol and acetone. After allowing the strip to air -dry,heat it for 30 min in an 
oven maintained at 105 °C ± 5 °C, cool it in a de siccator and immediately  determine its mass to the nearest 
0.1 mg.  
 
C.4.2 Prepare the test solution of the detergent or cleaning agent under test, at a concentration  recommended 
by the manufacturer, and transfer 250 mL of the freshly prepared solution to a  suitably stoppered glass bottle.  
 
C.4.3 Completely immerse the steel test strip in the solution in the glass bottle. Stopper the bottle  and heat it 
in an oven maintained at the temperature for the period as specified in the relevant  national standard.  
 
C.4.4 Remove the test strip from the test solution, rinse it thoroughly, first with water, then with  acetone, and 
allow it to air -dry. Then heat the test strip for 30 min in an oven maintained at105 °C ± 5 °C, cool it in the 
desiccator, and immediately determine its mass to the nearest 0,1 mg.  DRS  383: 2018  
13                ©RSB 2018 - All rights reserved  
  
C.5 Calculation  
Calculate the loss in mass in mg/100 mm2 of surface area as follows:  
 
  M=100
𝐴 𝑋 (𝑚1−𝑚2) 
 
Where , 
 
M  is the loss in mass of the steel test strip, in milligrams per 100 square millimetres;  
A  is the  total surface area, in square millimetres;  
m1 is the mass of the test strip before the test, in milligrams;  
m2 is the mass of the test strip after the test, in milligrams.  
C.6 Corrosiveness to aluminium  
C.6.1 Test strip  
 
One strip, of dimensions 75 mm × 19 mm  × 1.5 mm, of bright finished uncoated aluminium of a grade 
specified in the relevant national standard.  
 
C.6.2 Procedure  
 
NOTE   Should the mass loss evaluation method be required for the corrosiveness to aluminium, use the procedure 
given in C.4. 
 
C.6.2.1 Degrease the aluminium test strip by washing it in a mixture of equal volumes of ethanol and acetone. 
Allow the strip to air -dry, then heat it for 15 min in an oven at 105 °C ± 5 °C and allow it to cool in a desiccator.  
 
C.6.2.2 Prepare the test solution of the detergent or cleaning agent under test, at a concentration 
recommended by the manufacturer, and transfer 250 mL of the freshly prepared solution to a suitably 
stoppered glass bottle.  
 
C.6.2.3 Completely immerse the aluminium test strip in t he test solution in the bottle. Stopper the bottle and 
heat it in an oven maintained at the temperature for the period as specified in the relevant national standard.  
 
C.6.2.4 Remove the test strip from the test solution, rinse it thoroughly with water and  then acetone and allow 
it to air -dry. Then heat the test strip for 30 min in an oven at 105 °C ± 5 °C and cool it in a desiccator.  
C.6.2.5 Remove the test strip, and then visually inspect it for evidence of pitting, etching or discoloration.   
©RSB 2018 - All rights reserved                  14 
 Annex  D 
(normative)  
 
Consistency and storage stability  
C.1 Separately store samples of the product  in their original unopened containers at 5 °C ± 1 °C and at 43 °C 
± 1 °C for 48 h. Test the contents of the containers for compliance with the requirements of 4.7.1.  
 
C.2 Store samples of the product  at 23 °C ± 2 °C/50 %RH for a period of 12 months from the date of manufacture 
and then test the content of the containers for compliance with 4.2 and 4.7.2.  DRS  383: 2018  
15                ©RSB 2018 - All rights reserved  
 Annex  E 
(normative)  
 
Determination of foaming properties  
E.1 Accurately transfer 10 mL of the product  and 40 mL of the standard hard water to a 100 mL measuring 
cylinder with a stopper.  
E.2 Shake the cylinder vigorously for 30 s.  
 
E.3 After 60 s, examine the contents of the measuring cylinder for compliance with 4.8.  
 
 
 DRS  383: 2018  
ICS 11.080.20  
 
 
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