DRAFT UGANDA STANDARD  
  DUS DEAS 878 
First  Edition  
2016-mm-dd 
 
 Reference number  
DUS DEAS 87 8: 2016 
© UNBS 2016 
   
Antibacterial bathing bars — Specification  
 
 DUS DEAS 878: 2016 
ii                © UNBS  2016 - All rights reserved 
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2016 
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS. 
Requests for permission to reproduce this document should be addressed to 
The Executive Director 
Uganda National Bureau of Standards 
P.O. Box 6329 
Kampala 
Uganda 
Tel: +256 417 333 250 /1/2/3 
Fax: +256 414 286 123 
E-mail: info@unbs.go.ug  
Web: www.unbs.go.ug  
 DUS DEAS 878 : 2016 
© UNBS  2016 - All rights reserved                iii National foreword 
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade, Industry and 
Cooperatives established under Cap 327, of the Laws of Uganda, as amended.  UNBS is mandated to co-
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT Agreement of the World Trade Organisation (WTO). 
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of representatives of 
consumers, traders, academicians, manufacturers, government and other stakeholders. 
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
This Draft Uganda Standard, DUS DEAS 878:2016 , Antibacterial bathing bars — Specification, is identical 
with and has been reproduced from an International Standard, EAS 878: 2016 , Antibacterial bathing bars — 
Specification , and is being proposed for adoption as a Uganda Standard. 
This standard was developed by the Chemicals and environment Standards Technical Committee (UNBS/TC 
5). 
Wherever the words, “ East African Standard " appear, they should be replaced by "Uganda Standard."  DEAS 878 : 2016 
ICS 77.100.40  
© EAC 2016                                                                                                                         First Edition 2016  
 
DRAFT EAST AFRICAN STANDARD 
Antibacterial bathing bars — Specification  
EAST AFRICAN COMMUNITY DEAS 878: 2016 
ii © EAC 2016  – All rights reserved  
 Copyright notice 
This EAC document is copyright-protected by EAC. While the reproduction of this document by 
participants in the EAC standards development process is permitted without prior permission from EAC, 
neither this document nor any extract from it may be reproduced, stored or transmitted in any form for 
any other purpose without prior written permission from EAC. 
Requests for permission to reproduce this document for the purpose of selling it should be addressed 
as shown below or to EAC’s member body in the country of the requester:  
© East African Community 2016  — All rights reserved 
East African Community  
P.O.Box 1096 
Arusha 
Tanzania 
Tel: 255 27 2504253 /8 
Fax: 255 27 2504481/2504255 
E-mail: eac@eachq.org 
       Web: www.eac-quality.net 
Reproduction for sales purposes may be subject to royalty payments or a licensing agreement. 
Violators may be persecuted 
 DEAS 878: 2016 
© EAC 2016  – All rights reserved  iii 
 Foreword 
Development of the East African Standards has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
In order to achieve this objective, the Community established an East African Standards Committee mandated 
to develop and issue East African Standards.   
The Committee is composed of representatives of the National Standards Bodies in Partner States, together 
with the representatives from the private sectors and consumer organizations. Draft East African Standards 
are circulated to stakeholders through the National Standards Bodies in the Partner States. The comments 
received are discussed and incorporated before finalization of standards, in accordance with the procedures 
of the Community. 
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards are therefore expected to ensure that they always have the latest versions of the standards 
they are implementing.  
CD-K- 05 was prepared by Technical Committee EAS/TC 074, Surface active agents . 
 DEAS 878: 2016 
iv © EAC 2016  – All rights reserved  
 Introduction 
Performance of soaps, for a long time, has been primarily based on Total Fatty Matter (TFM).  
Whereas the above is a fact, technological trends have shown that performance of soap can be enhanced by 
acceptable and safe surface active agents where TFM levels have been reduced.  
In this standard the TFM levels have been reduced from that of antibacterial toilet soap with introduction of 
surface active agents whilst serving the same purpose. However this does not necessarily imply substitution 
of antibacterial toilet soap but rather an alternative and affordable antibacterial soap product.  
This standard therefore sets minimum requirements for performance and safety characteristics of antibacterial 
bathing bar. 
  DRAFT EAST AFRICAN STANDARD  DEAS 878: 2016  
 
© EAC 2016  – All rights reserved  1 
 Antibacterial bathing bars — Specification  
1 Scope 
This Draft East African Standard specifies the requirements and methods of sampling and test for solid 
antibacterial bathing bars.  
This standard applies to antibacterial bathing bars cakes or bar supplied in the form of cakes and produced 
from vegetable or animal oils or fats, fatty acids, or from a blend of all or part of these materials, with or 
without the addition of rosins or non-soapy surfactants. 
2 Normative references 
The following referenced documents are indispensable for the application of this document. For dated 
references, only the edition cited applies. For undated references, the latest edition of the referenced 
document (including any amendments) applies. 
 EAS 127, Synthetic laundry detergents for household use — Specification  
 EAS 377-1, Cosmetics and cosmetics products — Part 1: List of substances prohibited in cosmetic products 
 EAS 377- 2, Cosmetics and cosmetics products — Part 2: List of substances which cosmetic products must 
not contain except subject to restrictions laid down  
 EAS 377-3, Cosmetics and cosmetics products — Part 3: List of colourants allowed in cosmetic products 
 EAS 377-4, Cosmetics and cosmetics products — Part 4: List of preservatives allowed in cosmetic products 
 EAS 377-5, Cosmetics and cosmetics products — Part 5: Use of UV filters in cosmetic products  
 EAS 766-2, Antibacterial toilet soap – Specification- Part 2: Liquid 
EAS 794, Determination of the microbial inhibition of cosmetic soap bars and liquid hand and body washes — 
Test method  
 EAS 814,  Determination of biodegradability of surfactants — Test method  
 ISO 456, Surface active agents — Analysis of soaps — Determination of free caustic alkali  
 ISO 685, Analysis of soap — Determination of alkali content and total fatty matter content  
ISO 4315, Surface active agents -- Determination of alkalinity -- Titrimetric method 
3   Term and Definition 
For the purpose of this standard the following definition shall apply:  
antibacterial bathing bar 
is a product in the form of a bar or a cake containing an antibacterial agent and soap of fatty acids and 
synthetic surface active agents listed in 5.4.1 as active ingredients and which could be used for bathing 
purposes in soft and hard water.  
2 © EAC 2016  – All rights reserved  
 4 Requirements  
4.1   General requirements 
4.1.1  Antibacterial  bathing bars shall be in the form of cakes or bars.  
4.1.2  The colour of the cake or bar shall generally be uniform, but uniformity of colour shall not be obligatory 
in the case of genuinely mottled products.  
4.1.3 The product shall not be injurious to health if used in a manner and purpose meant for their use or 
under reasonably foreseen conditions. 
4.1.4 The cake or bar shall not have an unpleasant odour. 
4.1.5  The antibacterial  bathing bars shall be firm and smooth in texture.  
4.1.6  They are for personal washing purposes, and primarily contain saponified fatty matter, structurants, 
preservatives and moisture. They may also contain suitable quantities of colouring matter, perfume, opacifiers 
and optical brightening agents. 
4.1.7  Antibacterial  bathing bars shall not contain any ingredients in amounts that are harmful to the human 
body and environment 
4.1.8 Synthetic surface active agents, may be used and when used shall not be more than  4% by mass. 
when tested in accordance to Annex F 
4.1.9 In addition to moisture, normal colouring matters, preservatives acceptable in general, the 
antibacterial bathing bar shall contain permitted antibacterial agent . The label shall clearly state the 
antibacterial agent used and its level. 
4.1.10 The antibacterial bathing bar shall pass the antibacterial activity test when determined by the method 
given in EAS 794.  
4.1.11  Antibacterial bathing bars are for personal bathing purposes, and primarily contain antibacterial agent, 
saponified fatty matter, structurants, preservatives and moisture. They may also contain suitable and safe 
quantities of colouring matter, perfume, opacifiers and optical brightening agents.  
4.2 Specific quality requirements 
4.2.1   Ingredients  
The antibacterial bathing bar shall have one or more of the following surfactants conforming to the relevant 
East African Standard subject to the restrictions indicated:  
4.2.1.1  There is no restriction on the use of soap of fatty acids, fatty acid ester sulphonates, fatty 
alkalonamide, fatty alcohol ethoxylates, sarcosinates, taurides, fatty isothionates, alpha olefin sulphonates, 
alcohol sulphates and amphoterics such as betaines and fatty alcohol ethoxy sulphate, linear alkyl benzene 
sulphonates (LAS) and alkyl poly glycosides (APG) and fatty alcohol sulphosuccinate and fatty alkanol amido 
sulphosuccinate.  
4.2.1.2  In addition to the surfactants and perfume, the antibacterial bathing bar may contain other 
Ingredients such as electrolytes, bar structuring and processing aids, colouring matter, permitted antioxidants, 
preservatives, permissible germicides, super fatting agents, humectants, plant extracts. 
4.2.1.3  Rosins, as % of total fatty matter, shall not exceed 2 %. 
4.2.1.4  The synthetic surface active agents shall pass the biodegradability test as given in EAS 814.  DEAS 878: 2016 
© EAC 2016  – All rights reserved  3 
 4.2.2  All ingredients shall be declared on the lab el following descending order in terms of quant ity. 
4.2.3  All the ingredients used in the antibacterial bathing bars shall comply with the requirements of all parts 
of EAS 377. 
4.2.4  Performance and safety requirements   
Antibacterial bathing bars shall also comply with the requirements given in Table 1 when tested against the 
methods prescribed therein.  
Table 1 — Performance and safety requirements 
 
SL. No . Characteristic  Requirement  Test method  
i)  Total fatty matter, % by mass, 
minimum.  50 ISO 685  
ii)  Lather , mL, min.  200 Annex A 
iii)  Mush (loss in mass due mushing 
on a wet surface) (g/30  cm2, max).  10 Annex B 
iv)  Freedom from grittiness  To pass the test  Annex C 
v)  Total alkalinity (as NaOH) % by 
mass, max.  1.0 ISO 685  
ISO 4315  
vi)  Free caustic alkali (as NaOH), % 
by mass, max.  0.05 ISO 456  
vii)  Triclosan (TCN) and 
Trichlorocarbanilide (TCC)  Shall not exceed 1 
% by mass  Annex D. 
viii)  Chloro aniline content  shall not exceed 10 
ppm Annex E. 
NOTE:  Trichlorocarbanilide (TCC) is not heat stable and decomposes into chloro anili nes on 
prolonged heating above 60 °C. If TCC is used in soap, the manufacturer should take care that such 
soap is not subjected to temperature above 60 °C during the entire manufacturing process or during 
storage  
 
 
6  Packaging and labelling 
6.1  Packaging  
Each cake or bar may be unwrapped or may be wrapped. Unwrapped or wrapped bars shall be packed in 
suitable boxes, packages or cartons to avoid contamination or damage during transportation.   
6.2  Labelling 
Each antibacterial bathing bar shall be marked legibly and indelibly with the following particulars:  
a) the words “Antibacterial Bathing Bar ”; 
b) manufacturer’s name and physical address;  
NOTE The name, physical address of the distributor/supplier and trade mark may be added as required.  
4 © EAC 2016  – All rights reserved  
 c) nominal weight of each bar or cake at the time of packaging; 
d) number of bars or cakes contained in the package; 
e) batch number or code number; 
f) all ingredients except moisture in descending order of quantity; 
g) date of manufacture and best before date; 
h) anti bacterial agents used and their levels; 
i) country of origin. 
7 Sampling  
Sampling shall be done in accordance to with EAS 766-2, Annex D. 
  DEAS 878: 2016 
© EAC 2016  – All rights reserved  5 
  
 
Annex A  
(normative)  
 
Test for lather volume 
A.1 General 
Strict attention shall be paid to all details of the procedure in order to ensure concordant results. Particular 
care should be taken to invert the cylinder exactly as described. 
A.2 Outline of the method 
A suspension of the material in standard hard water is taken in a graduated cylinder and given 12 inversions 
under prescribed conditions. The volume of the foam formed is observed after keeping the cylinder for 5 
minutes. 
A.3 Reagents 
A.3.1  Calcium chloride CaCl 2.2H 2O, AR 
A.3.2  Magnesium sulphate MgSO 4.7H 2O, AR 
A.3.3  Distilled water  
A.4 Apparatus 
A.4.1  Graduated cylinder  — Glass stoppered with graduation from 0 to 250 mL, with 2 mL divisions. 
Overall height about 35 cm and the height of the graduated portion about 20 cm. 
A.4.2  100-mL glass beaker  
A.4.3  Thermometer  of range 0 - 110◦C  
A.5 Preparation of standard hard water 
Dissolve 0.220 g of calcium chloride dihydrate and 0.246 g of magnesium sulphate heptahydrate in distilled 
water. Dilute to 5 L with distilled water. 
NOTE This standard hard water has a hardness of approximately 50 ppm calculated as calcium carbonate. 
A.6 Sample preparation 
Cut away the outer edges of bathing bar using a knife 
Using a stand up type of grater, grate up to 10 g – 15 g of the bathing bar into small chips.  
6 © EAC 2016  – All rights reserved  
 A.7 Procedure   
Weigh 1 g of the grated chips antibacterial bathing bar accurately in a 100-mL glass beaker. Add 10 mL of the 
standard hard water. Cover the beaker with a watch glass and allow to stand for 30 min. The operation is 
carried out to disperse the antibacterial bathing bar .  
Stir the contents of the beaker with a glass rod and transfer the slurry to a 250-mL graduated cylinder 
ensuring that not more than 2 mL foam is produced. Repeat the transfer of the residue left in the beaker with 
further portions of 20 mL of standard hard water ensuring that all the matter in the beaker is transferred to the 
cylinder. 
Adjust the contents in the cylinder to 100 mL by adding sufficient standard hard water. Bring the contents of 
the cylinder to 30 ◦C. Stir the contents of the cylinder with a glass rod or thermometer to ensure a uniform 
suspension. 
As soon as the temperature of the contents of the cylinder reach 30 ◦C, stopper the cylinder and give it 12 
complete inversions, each inversion comprising movements in a vertical plane, upside down and vice versa. 
After the 12 inversions, let the cylinder stand for 5 min. Take the following readings as shown in Figure A.1: 
a) foam plus water (V 1 mL). 
b) water only (V 2mL). 
 
Figure A.1 — Measurement of foam DEAS 878: 2016 
© EAC 2016  – All rights reserved  7 
 A.8 Calculation 
Lather volume = V1 - V 2 
where 
 V 1 = Volume, in mL of foam + water; 
 V 2 = Volume, in mL of water only.  
8 © EAC 2016  – All rights reserved  
 Annex B  
(normative)  
Evaluation of the mushing properties of a bathing bar  
B.1  Principle  
A test piece of defined size is cut from the sample bar to remove harder outer layers. The test piece is 
preconditioned by giving 18 x 180 degree twists under running water at 25 °C or in a bowl of water at 25 °C. 
The bar is left for six hours on a piece of fabric that has been wetted and drained of excess water. During the 
six hours the soap/ cloth are covered to prevent drying.  At the end of the test period mush is removed from 
the test piece face in contact with the cloth. Weight loss from the test piece is expressed as mush per 30 cm2
 
of original surface area in contact with the cloth.  
B.2  Equipment  
B.2.1  For sample preparation  
B.2.1.1 Coarse kitchen cheese grater  
 
B.2.1.2 Sharp thin blade knife or carpenters plane  
 
B.2.1.3 Callipers or ruler,  to ensure the sample dimensions  
 
B.2.1.4  Other equipment/ materials for the test  
Plastic or non-corrodible trays which are suitable sized for the test piece. Plastic soap dishes 7 cm x 11 cm x 2 
cm are quite suitable.  
Cotton cloth pieces cut and folded to fit as a triple layer inside the trays. Normal, flat weave, cotton sheeting 
as used for bed sheets will be quite suitable.  
B.3  Bar preparation  
B.3.1  Three (3) individual bars of a type should be tested. A test piece is cut from each bar. The test piece 
should if possible have a working face (to be applied to the fabric) of 6 cm ±1 cm x 4 cm ±1 cm.  
All bars in a set shall be cut to have the same face size. If the smallest of the range of bars to be tested at a 
given time is too small to allow a working face within these limits, then all bars should be cut to the maximum 
size possible from the smallest bar.  
The longest axis of the test piece (6 ±1) cm should be from a direction parallel to the longest axis of the 
original bar sample.  
The working face should be a fresh surface from the interior of the bar sample. The face opposite the working 
face should be identified by making a small hole with a sharp object. This enables the working face to be 
identified after the preconditioning step.  
B.3.2  To cut the bar it is convenient to first trim it to the approximate size using a coarse kitchen cheese 
grater and then to make the final adjustments to a smooth surface with a sharp thin-bladed knife or carpenters 
plane. If a plane is used, it is better to move the bar over the plane blade.  
B.4  Test procedure  
 
For each test piece  
 DEAS 878: 2016 
© EAC 2016  – All rights reserved  9 
 B.4.1  The tray plus triple thickness of cloth is filled with demineralised water. The tray is then held vertically 
to drain the water from the cloth. The vertical position is maintained until water ceases to run from the dish in a 
continuous stream i.e. starts to drip.   
 
B.4.2  The area of the working face of the test piece is measured (A).  
 
B.4.3  The working face of the bar is placed onto the damp fabric and then the tray plus soap are covered 
e.g. with a sealed plastic bag, to prevent water loss.  
 
B.4.4  The covered test piece and holder are maintained at 25 oC for 6 h.  
 
B.4.5  The mushed soap test piece is removed from the tray and is weighed (W1).  
 
B.4.6  Mush is removed from the working face of the soap test piece by scraping with the edge of a blunt 
sided spatula or plastic ruler.  
 
B.4.7  The test piece is reweighed (W2) and the amount of mush removed is calculated as in D.5.  The 
mush is expressed as grams per 30cm2
 of original test piece surface area.  
 
NOTE   The procedure for weighing the bar and removing the mush will take some minutes. During that time the 
remaining soaps will continue to form mush. While this time is not critical for a set of three test pieces from a given 
product, if more than one product is under test it is advised to stagger the start of the test for the second product. This will 
give adequate time to complete work on the first set before the 6-hour storage time of the subsequent set is completed. 
 
B.5  Calculation  
 
Weight of mush (grams) W = W1 – W2 
 
Surface area of bar (cm2) A = (width x breadth) 
 
Mush =
Ag30W per 30 cm2 
  
B.6  Criteria for conformity  
The test is done with three (3) separate samples of each product type, and the mean value from three 
samples is quoted (X). The range of values (R) is quoted as the difference between the highest and lowest 
values obtained for a given product type.  
The sample lot of products shall be declared as conforming to the requirements for this standard if X + 0.6R is 
less than the maximum value given in Table 1.   
10 © EAC 2016  – All rights reserved  
 Annex C  
(normative)  
Determination of grittiness in a Antibacterial bathing bar  
 
 
C.1  Procedure  
Either  
Hold the antibacterial bathing bar under a smooth stream of running water at a temperature of 30 °C and 
gently rub the two sides of the bar on the palm of one hand for one minute each side.  
or  
Immerse the soap in a bowl containing 5 L of water at 30 °C and gently rub two opposite bar faces with the 
palm of one hand for 30 s (15 s per bar face). Remove the bar from the water and continue to gently rub the 
two opposite bar faces for a further 30 s (15 s per face). 
Allow the used bar to dry in the open for 4 hours and examine the surface.   
A set of 3 samples will be tested for each product.  
NOTE 1: Hands will become hydrated and insensitive with prolonged immersion in water.  Testers should wait 15 min 
between testing every 3 sets of products (9 grit tests).   
NOTE 2:  If using a bowl rather than running water use fresh water after testing every set of 3 samples.  
C.2  Criteria for conformity  
The performance criteria are:  
During manipulation under running water the washing bar will not have a visibly rough surface and will feel 
smooth to the touch. No gritty particles will be observed on the surface of the dried bar 4 h after the washing 
test. DEAS 878: 2016 
© EAC 2016  – All rights reserved  11 
 Annex D 
(normative) 
Determination of TCC and TCN in antibacterial bathing bar by HPLC 
 
D.1 Principle 
TCC and TCN are antibacterial agents, which are separated from other components in soap by normal phase 
or reverse phase liquid chromatography, detected spectrophotometrically and quantified by comparison with 
standard TCC and TCN.  The method can estimate as low as 1 ppm of the above compounds. 
Procedures for both normal and reverse HPLC has been described and provide the option to use either 
method whichever is available to the users. Both methods are comparable.  
D.2 Normal phase HPLC  
D.2.1 Reagents  
D.2.1.1 Iso-octane –  HPLC grade 
D.2.1.2  Iso-propanol (2 propanol) – HPLC grade 
D.2.1.3  Hexane – HPLC grade 
D.2.1.4  Standard TCC – 99 % pure 
D.2.1.5  Standard TCN – 99 % pure 
D.2.2 Apparatus  
D.2.2.1  High performance liquid chromatograph  – Consisting of a pump, a sample injector of fixed 
volume with UV detector having variable wavelengths and a recorder. 
D.2.2.2  Standard volumetric flasks  
D.2.2.3  Pipettes 
D.2.2.4  Magnetic Stirrer  
D.2.2.5  Millipore Filter Apparatus with 0.5 µ Filter  
D.2.2.6  Column 
D F.2.2.6.1 Silica column 
Stainless steel 25 cm x 0.46 cm packed with Normal Phase – Silica 5 µ (Lichrosorb Si-60) 
D F.2.2.6.2 Cyano column  
Stainless steel  25 cm x 0.40 cm packed with (Lichrospher 100) cyano 5 µ. 
NOTE    Either of the above columns can be used depending on the availability.  
12 © EAC 2016  – All rights reserved  
 D.2.2.7  Mobile Phase 
D.2.2.7.1 For silica column  — Transfer 20 mL of iso-propanol into a 500 mL volumetric flask and make 
up to mark with iso-octane and mix well.  Assemble millipore filter apparatus and filter the solvent system prior 
to use.    
D.2.2.7.2  For cyano column — Transfer 50 ml of HPLC grade iso-propanol (2-propanol) into a 500 mL 
volumetric flask, fill upto the mark with hexane and mix well. Assemble millipore filter apparatus and filter the 
solvent system prior to use. 
D.2.2.8 HPLC Conditions  
Detector wavelength  : 280 nm 
Flow rate    : 0.5 m  L/min 
Injection volume  : 20 µ L 
Retention time 
Silica column  
TCN - 7.5 minutes 
TCC - 19.2 minutes 
Cyano column  
TCN - 4.0 minutes 
TCC -  7.5 minutes 
D.2.3  Procedure 
D.2.3.1  Standard preparation (see note under F.3.4) 
Weigh accurately 25 mg of triclosan (TCN) and 25 mg of TCC into a 100 ml volumetric flask and make up to 
volume with the mobile phase and mix well. Pipette 1.0 mL of this solution in a 50 mL volumetric flask and 
dilute with mobile phase. Final concentration of TCC and TCN is 250 µg/50 mL (5.0 ppm). 
D.2.3.2  Sample preparation  
Weigh accurately 1g of homogenized sample into a 100- mL standard flask, and dilute to the mark with mobile 
phase.  Pipette 10 mL of the supernatant liquid to a 50- mL volumetric flask, dilute with mobile phase, to the 
mark, and filter through 0.45 µm filter. 
D.2.3.3  Chromatography  
Equilibrate the column, maintained at a temperature of 30ºC, with the mobile phase with a flow rate of 0.5 
ml/min for iso-octane-iso-propanol mobile phase and 1.0 mL/min for hexane – iso-propanol mobile phase for 
30 minutes. Set the wavelength at 280 nm. Inject 20µL of standard solution and then sample solutions. 
Measure area of the peaks of respective retention time for standard and sample. 
D.2.4  Calculation 
 TCN, percent by mass =   Area of sample for TCN × Concentration of standard TCN ×100 
 DEAS 878: 2016 
© EAC 2016  – All rights reserved  13 
          Area of standard TCN × Concentration of sample 
 
 TCC, percent by mass =   Area of sample for TCC× Concentration of standard TCC ×100 
 
           Area of standard TCC × Concentration of sample 
D.3  Reverse phase 
D.3.1  Reagents 
D.3.1.1  Methanol – HPLC grade  
D.3.1.2  Sodium Dihydrogen Phosphate Monohydrate – Chemical grade. 
D.3.1.3  Standard TCC 
D.3.1.4  Standard TCN (TCS) 
D.3.1.5 10 percent phosphate solution  
D.3.2  Apparatus 
D.3.2.1  Column – Octyldimethylsilyl (C-DB) Supercosil LC-8-DB – 15 cm x 4.6 mm. 5µ.  
D.3.2.2  Mobile Phase 
MeOH/0.01 M Phosphate buffer 62:38 v/v .  
D.3.2.2.1   Preparation of 0.01M Phosphate buffer solution :  
Dissolve 1.38 g sodium dihydrogen phosphate monohydrate in 1000 mL of distilled water.  Adjust the pH of 
the mobile phase to 3.0 using 10 percent phosphate solution.  
D.3.3   Procedure 
D.3.3.1  Standard preparation (see Note under F .3.4) 
D.3.3.1.1  Weigh accurately about 90 mg TCN. Dissolve in methanol and make up to 1 000 mL volumetric 
flask with methanol. 
D.3.3.1.2   Weigh about 110 mg of TCC, dissolve well with methanol, and make up the volume to 1 000 ml. 
D.3.3.1.3   Accurately pipette 10 ml of the solution prepared in (see D.3.3.1.1) into the (see D.3.3.1.2) 
volumetric flask containing TCC. And make up to the volume with methanol. Then accurately pipette 5-ml of 
the solution into a 50-ml volumetric flask. Make up to the volume with methanol. Filter this standard solution 
through 0.45 µm filter. 
D.3.3.2  Sample preparation 
Weigh accurately about 1.0 g of product, dissolve in methanol and make up to 100 ml in a volumetric flask 
with methanol. Filter this sample solution through 0.45 µm filter. 
D.3.3.3  HPLC Conditions 
Detector wavelength :  280 nm  
14 © EAC 2016  – All rights reserved  
 Column temperature :  35°C 
Flow rate            :  1.0 mL/min 
Injection volume          :  10 µL 
Prepare the standard solution and the sample solution at the same time. Inject the standard solution three 
times and calculate the average of each ingredients peak count. Inject 10 µL the sample solution and 
determine each ingredients percentage by the calculation shown. 
D.3.4    Calculations  
      TCN percent by mass =       
100tMsAFrAsM
  
 
TCC percent by mass =
10tMsAFrAsM

  
     
where 
Ms is the Mass of the standard (g), 
As  is the Averaged peak of the area of the standard, 
Mt is the Mass of the test sample (g), 
Ar  is the Peak area of the test sample, and 
F is the Purity of standard (percent). 
NOTE: Both TCC and TCN are photosensitive, hence standards should be freshly prepared. DEAS 878: 2016 
© EAC 2016  – All rights reserved  15 
  
Annex E 
(normative) 
Determination of chloroaniline 
E.1  Principle 
The chloroanilines are extracted from soap with dimethyl sulfoxide and diazotized with nitrous acid. The 
reaction products are then coupled with N-1-(naphthyl) ethylenediamine hydrochloride to produce coloured 
compounds which are estimated spectrophotometrically. 
E.2  Safety precautions 
Dimethyl sulfoxide (DMSO) is readily absorbed into the skin. Inhalation or skin penetration shall be avoided. 
DMSO should never be pipette using mouth. Always use pipette bulb. The standard chloroanilines and N-1-
(naphthyl) – ethylenediamine hydrochloride shall not be allowed to come into contact with the skin. If they 
should, then wash the contaminated parts thoroughly with soap and water. 
A supply of diluted sodium hypochlorite should be at hand at all times to deal with accidental spillages of 
chloraniline solution. Spillage on laboratory surface should be treated immediately with the sodium 
hypochlorite solution, followed by water. 
E.3   Reagents 
E.3.1    Dimethyl sulphoxide (DMSO)  – AR grade 
E.3.2   Hydrochloric a cid – Concentrated (specific gravity – 1.18)  
E.3.3   Sodium nitrite  – 0.4 %w/v analytical grade, freshly prepared (aqueous). 
E.3.4   Ammonium sulphamate  – 2 % w/v solution freshly prepared, (aqueous). 
E.3.5   N-1-(naphthyl) Ethylene  – 0.1 % w/v solution diamine hydrochloride freshly prepared  (aqueous). 
E.3.6   n-Butanol  – AR grade 
E.3.7   Sand  – Acid purified 40 – 100 micron mesh. 
E.3.8   Solvent Mixture 
DMSO       5 Volumes 
n-Butanol      2 Volumes 
distilled water      2 Volumes 
hydrochloric acid  1 Volume 
Mix n -butanol, water and HCl Cool the mixture and add DMSO (5 Volumes). 
E.3.9   4- Chloroaniline and 3, 4-Dichloroaniline  – AR grade 
E.4   Apparatus  
16 © EAC 2016  – All rights reserved  
 E.4.1    Spectrophotometer  – Suitable for use at 554 nm. 
E.4.2  Cuvettes – Glass (matched pair) 10 mm. 
E.4.3  Water bath  – Thermostatically controlled at 25 ºC 
E.4.4  Stop watch 
E.4.5  Standard laboratory glassware 
E.4.6  Filter paper – Whatman No. 541 
E.5  Procedure 
E.5.1  Preparation of Calibration Curve 
E.5.1.1   Dissolve 0.349 8 g of 3, 4-dichcoroaniline and 0.275 3 g of 4-chroroaniline in solvent mixture (see 
G.2.8) in a 250 mL amber volumetric flask. Dilute to mark with solvent mixture. 1 mL = 2.5 mg mixed  
chloroanilines (stock solution). 
E.5.1.2   Dilute this stock solution with solvent mixture as given below: 
a) Take 5 ml of stock solution and dilute it to 250 mL with mixture. 
1 mL = 50 µg mixed chloroanilines. 
 
b) Take 5 ml of the above solution (see G.5.1.2 (a)) and further dilute to 250 mL. With solvent mixture. 
1 mL = 1 µg mixed chloroanilines. 
 
Use this solution for preparation of calibration curve. 
Transfer using a burette 0, 1, 2, 5, 10, 20, 40 mL into 50 mL amber volumetric flasks. 
E.5.1.3   From a burette, add sufficient solvent mixture to make total volume to 40- mL in each flask. The flasks 
are incubated in a water bath at 25ºC for 20 min. After exactly 20 minutes, add 2- mL of reagent (see E.3.3) 
into each flask and return them to the water bath for exactly 10 min (measure with a stop watch). 
Then add 2- mL of reagent (see E.3.4) into each flask and return them to the water bath for exactly 10 minutes. 
Swirl the flask occasionally. 
Then add 2- mL of reagent (see E.3.5) into each flask and remove them from the water bath. Dilute to volume 
with distilled water, mix and allow to stand for 30 minutes. Measure absorbance at 554 nm against the blank 
solution as prepared in E.5.1.4. 
E.5.1.4   Preparation of blank solution  
Take 40 mL of solvent mixture in a 50 mL amber volumetric flask. Incubate the flask in water bath at 25ºC for 
20 min. After exactly 20 min, add 2 ml of reagent (see E.3.3) into the flask and return it to the water bath for 
exactly 10 minutes. Then add 2 mL of reagent (see E.3.4) into the flask and return it to the water bath for 
exactly 10 minutes (Swirl the flask occasionally).  
Then add 2 mL of reagent (see E.3.5) into the flask and remove it from the water bath. Dilute to volume with 
distilled water, mix and allow to stand for 30 minutes. Use this blank solution for preparation of calibration 
curve only. 
E.5.1.5   Prepare a graph by plotting weight (µg) of chloroanilines contained in each 50 ml flask against 
absorbance. The linear calibration will pass through the origin/or determine the average absorbance (AA) of 1 
µg of mixed chloroanilines by dividing sum of absorbances of all different aliquots of the standard by sum of 
µg of chloroanilines in all different aliquots of standard. DEAS 878: 2016 
© EAC 2016  – All rights reserved  17 
 E.6  Determination of chloroanilines 
E.6.1  Weigh to the nearest mg 3.0 - 3.5 g of finely grated antibacterial bathing bar add 10.0g – 15.0 g of 
acid purified sand. Transfer quantitatively the sample and the sand into a mortar and grind the mixture 
thoroughly with a pestle to give a homogenous mass.  
Transfer the mass to a pre viously weighed 250 mL flat bottom flask quantitatively and reweigh. Add DMSO 
(100 mL), stopper firmly and attach the flask to an automatic shaker.  Shake for 1 h. 
Filter the DMSO extract through whatman No.541 into a 250 mL amber volumetric flask. Wash the flask and 
filter paper with small aliquots of DMSO. Allow the filtrate to drain completely, dilute to volume with DMSO and 
mix. Transfer 20 mL DMSO extract into 50 ml amber volumetric flask.  
Add 20 mL of solvent mixture. The flask is incubated in a water bath at 25ºC for 20 minutes after exactly 20 
minutes, add 2 ml of reagent (see G.3.3) into the flask and return it to the water bath for exactly 10 minutes 
(measure with a stop watch). Then add 2 mL of reagent (see G.3.4) into the flask and return it to the water for 
exactly 10 minutes (swirl the flask occasionally).  
Then add 2 mL or reagent (see G.3.5) into the flask and remove it from the water bath. Dilute to volume with 
distilled water, mix and allow to stand for 30 minutes. Read the absorbance at 554 nm against blank (prepared 
as below). 
E.6.2  Preparation of Blank  
Prepare the blank solution by mixing 20 mL of DMSO extract of sample and 20 mL of solvent mixture in a 50 
ml amber volumetric flask. Incubate the flask in a water bath at 25 ºC for 20 min. 
 
After exactly 20 minutes, add 2 mL of distilled water into the flask and return it to the water bath for exactly 10 
minutes. Then add 2 mL of reagent (see E.3.4) into the flask and return it to the water bath for exactly 10 
minutes (swirl the flask occasionally).  
 
Then add 2 mL of reagent (see E.3.5) into the flask and remove it from the water bath. Dilute to volume with 
distilled water, mix and allow to stand for 30 min. Use the solution as a blank for reading sample only. 
 
E.6.3  Deduce the amount of chloroaniline (µg) from the calibration graph curve. 
NOTE – The determination shall be completed in one day 
E.7  Calculations 
 
E.7.1  Determine the amount of mixed chloroanilines in the aliquot of test solution from the calibration graph. 
 
Chloroaniline content (in ppm) =                  

M2M203M1MM250   
      
Where  
 
M   is the   mass in g of soap, 
  
18 © EAC 2016  – All rights reserved  
 M1  is the mass in g of sand, 
M2 is the mass in g of soap and sand transferred to the flask, 
M3  is the mass (µg) of mixed chloroanilines found from calibration graph/or it can be calculated as given 
below: 
    Mass of the sample 
M3   =   
Average absorbance of 1 µg mixed chloroanilines (AA) 
 
Where 
   Sum of the OD of the standards 
AA = 
  Sum of concentration of standard chloroanilies in µg 
 
Weight of soap actually used, in g =      
1MMM2M
  DEAS 878: 2016 
© EAC 2016  – All rights reserved  19 
 Annex F 
(normative) 
Determination of active detergent content  
F.1 Outline of the method 
When equivalent amounts of cationic and anionic detergents are present in a t wo-phase mixture of water and 
chloroform, methylene blue will colour the two phases to the same degree. Sodium alkyl benzene sulphonate and 
sodium lauryl sulphate or any other detergent can be titrated with a standard solution of cetyl trimethyl ammonium 
bromide. 
F.2 Reagents 
Weigh 1.5 ± 0.001 g of cetyl trimethyl ammonium bromide into a 250 mL beaker. Add 100 mL of distilled water 
and stir until dissolved. Transfer quantitatively to a 1 litre volumetric flask and make to volume. Mix thoroughly 
and s tandardize against solution B. (See F.2.1).  
F.2.1 Anionic solution (Solution B)  
Weigh accurately such amount of standard alkyl sulphate of known combined SO 3 or active content so as to 
give exactly 0.320 g of combined SO 3 into a 250 mL beaker.  Dissolve in 100 mL to 200 mL of warm water.  
Transfer quantitatively to 1-litre volumetric flask and make to volume with water at room temperature. Mix 
thoroughly.  This is the primary standard against which solution A, is standardized. Solution B is 0.004 N. 
F.2.2 Methylene blue indicator  
Dissolve 0.1 g of methylene blue in 100 mL of water. Transfer 30 mL of this solution to a 1-L flask. Add 500 
mL of water, 6.8 mL of concentrated sulphuric acid, 50 g of sodium dihydrogen phosphate monohydrate 
(NaH 2PO 4H2O) and shake until solution is complete. Dilute to the mark. 
F.2.3 Chloroform 
 
Analytical reagent grade  
F.3 Procedure 
F.3.1 Weigh accurately a sample of sufficient size to give approximately 0.320 g of combined SO 3 into a 250 
mL beaker. Sample size is crucial (see Note). Use 700 mL to 800 mL of warm water to transfer quantitatively 
to a 1-L volumetric flask. Warm on steam bath and shake gently until the sample is dissolved and solution is 
clear. Cool, dilute to the mark and mix thoroughly. 
NOTE The titration value V should be as near as to 10 mL as possible, say between 8 mL and 12 mL but never 
outside 5 mL and 15 mL. 
F.3.2 Pipette 10.0 mL of the sample solution into a 100 mL glass stoppered cylinder (25 x 300 mm). Add 
25.0 ± 0.5 mL of methylene blue solution and 10 ± 0.5 ml of chloroform (see Note). Titrate with solution A to 
the correct end point, shaking the cylinder carefully after such addition to avoid emulsion and maintaining 
temperature within prescribed limits of 20 C - 30 C by immersion in water bath, if necessa ry. As the end 
point is approached, the rate of transfer of colour increases and solution A shall be added dropwise with 
vigorous shaking after each addition. If the approximate titration is known, before shaking since this avoids 
emulsion formation.  
20 © EAC 2016  – All rights reserved  
 Application of vacuum to the titration cylinder may help to break some emulsions, if formed. The end point is 
reached when both layers have same colour intensity. The end point is very sharp and 0.05 mL will cause a 
distinct change in colour distribution at or near the equivalence point. 
NOTE The titration value V should be as near to 10 mL as possible, say between 8 mL and 12 mL but never outside 
5 mL and 15 mL. 
F.3.3 Pipette 10.0 mL of the sample solution into a 100-mL glass stoppered cylinder (25 x 300 mm). Add 
25.0 mL ± 0.5 mL of chloroform (see Note). Titrate with solution A to the correct end point, shaking the 
cylinder carefully after such addition to avoid emulsion and maintaining temperature within prescribed limits of 
20◦C  to 30◦C by immersion in water bath if necessary. As the end point solution A shall be added dropwise 
with vigorous shaking after each addition. If the approximate titration is known, 80 % of the required titrating 
solution should be added before shaking since this avoids emulsion formation. 
Application of vacuum to the titration cylinder may help to break some emulsions, if formed. The end point is 
reached when both layers have same colour intensity. The end point is very sharp and 0.05 mL will cause a 
distinct change in colour distribution at or near equivalence point. 
NOTE The volume of methylene blue solution and chloroform may be changed if found advantageous provided the 
same volumes are used in standardizing solutions A and B. 
F.3.4 Calculation 
F.3.4.1 The percent combined SO 3 shall be expressed as follows: 
% combined SO 3  =  
MNV 8.0 x   x  
where,   
V     volume, in millilitres, of solution A used in the titration; 
N     normality of solution A; and 
M     mass, in grams, of the sample in the aliquot. 
F.3.4.2  The percent active detergent content shall be expressed as follows: 
Percent active detergent content = percent combined SO 3 x Mol. weight of active detergent. 
NOTE The molecular weight of active detergent should be supplied by the manufacturer on request. 
F.4 Alternative  method for determination of active detergent content 
(To be used only if the first method (Clause H .1) fails to work on the product). 
F.4.1 Field of application 
This method is applicable to the analysis of alkylbenzene sulphonates, alkyl sulphonate, sulphates and 
hydroxy-sulphates, alkylphenol and fatty alcohol ethoxysulphates and dialkyl sulphosuccinates and to the 
determination of active materials containing one hydrophilic group per molecule. 
F.4.2 Principle 
Determination of anionic-active matter in a medium consisting of an aqueous and chloroform phase, by 
volumetric titration with a standard cationic-active solution (benzethonium chloride), in the presence of an 
indicator which consists of a mixture of a cationic dye (dimidium bromide) and an anionic dye (acid blue 1). DEAS 878: 2016 
© EAC 2016  – All rights reserved  21 
 F.4.3 Reagents 
F.4.3.1 The water used shall be of distilled quality. 
F.4.3.2 Chloroform, (sp. gravity = 1.48 g/m, distilling between 59.5ºC and 61.5ºC). 
F.4.3.3 Sulphuric acid, 2.5 M solution. 
F.4.3.4 Sulphuric acid, 0.5 M solution. 
F.4.3.5 Sodium hydroxide, 1.0 M standard volumetric solution. 
F.4.3.6 Sodium lauryl sulphate (sodium dodecyl sulphate) (CH 3(CH 2) (11 OSO 3Na), 0.004 M standard 
volumetric solution.  
Check the purity of the sodium lauryl sulphate and simultaneously prepare the standard solution. 
F.4.3.6.1  Determination of purity of sodium lauryl sulphate — Weigh to the nearest 1 mg, 5 g ± 0.2 g of the 
product into a 250-mL round bottom flask with ground glass neck. Add exactly 25 mL of the sulphuric acid 
solution (H.4.3.4) and reflux into a water condenser. 
During the first 5 min - 10 min, the solution will thicken and tend to foam strongly; control this by removing the 
source of heat and swirling the contents of the flask. 
In order to avoid excessive foaming, instead of refluxing the solution may be left on a boiling water bath for 60 
min. 
After a further 10 min the solution clarifies and foaming ceases. Reflux for further 90 min. Remove the source 
of heat, cool the flask and carefully rinse the condenser with 30 mL of ethanol followed by water. 
Add a few drops of the phenolphthalein solution (F.4.3.8) and titrate the solution with the sodium hydroxide 
solution (F.4.3.5). 
Carry out a blank test by titrating 25 mL of the sulphuric acid solution (F.4.3 .4) with the sodium hydroxide 
solution (F.4.3.5). 
The purity of the sodium lauryl sulphate, expressed as a percentage,  
1O O 1   ) ( 28.84
MM VV  
 
where, 
V0…=  volume, in millilitres, of sodium hydroxide solution used for the blank test; 
V1  .= volume, in millilitres, of sodium hydroxide solution used for the sample; 
m1  ..= mass, in grams, of the sodium lauryl sulphate to be checked; and  
M0   = exact molarity of the sodium hydroxide solution. 
F.4.3.6.2  Weigh 0.004 M sodium lauryl sulphate standard volumetric solutions. Weigh, to the nearest 1 mg 
between 1.14 g and 1.16 g of sodium lauryl sulphate and dissolve in 200 mL of water. Transfer to a ground 
glass stoppered 1-L one-mark volumetric flask and dilute to the mark with water. 
Calculate the molarity, M 1, of the solution by means of the solution by means of the formula:  
22 © EAC 2016  – All rights reserved  
 
100 x 288.4(%)  x   2
1purity m   M 
where,  
m2 = mass in grams of sodium lauryl sulphate. 
F4.3.7 Benzethonium chloride 0.004 M standard volumetric solution  
Weigh, to the nearest 1 mg, between 1.75 g and 1.85 g benzethonium chloride and dissolve in water. Transfer 
to a ground glass-stoppered 1-L one-mark volumetric flask and dilute to the mark with water. 
NOTE In order to prepare a 0.004 M solution, dry the benzethonium chloride at 105EC, weigh 1.792 g, to the nearest 
1 mg, dissolve in water and dilute to 1 L. 
F.4.3.8 Phenolphthalein, ethanolic solution containing 10 g/L. Dissolve 1 g of phenolphthalein in 100 mL 
of 95 % (v/v) ethanol. 
F.4.3.9 Mixed indicator 
F.4.3.9.1 Stock solution  
Weigh to the nearest 1 mg 0.5 g ± 0.005 g dimidium bromide into a 50-mL beaker, and 0.025 g ± 0.005 g of 
acid blue 1 into a second 50-mL beaker.  
Add between 20 mL and 30 mL of hot 10 %. (v/v) ethanol to each beaker. Stir until dissolved and transfer the 
solutions to a 250-mL one mark volumetric flask. Rinse the beakers into the volumetric flask with ethanol and 
dilute to the mark with 10 % (v/v) ethanol. 
F.4.3.9.2 Mixed acid indicator solution  
Take 20 mL of the stock solution prepared above, put it in a 500-mL one-mark volumetric flask. Add 200 mL of 
water, and 20 mL of 2.5 M sulphuric acid (F.4 .3.3) mix and dilute to the mark with water. Store away from 
direct sunlight. 
F.4.4 Apparatus 
Ordinary Laboratory apparatus, and  
a) bottles, 200-mL, glass stoppered, or measuring cylinders,  glass stoppered. 
b) burettes, 25-mL and 50-mL. 
c) one-mark volumetric flask, 1-L capacity glass stoppered. 
d) one-mark pipette, 25-mL. 
F.4.5 Procedure 
F.4.5.1  Standardization of benzethonium chloride solution 
By means of the pipette transfer 25 mL of the 0.004 M sodium lauryl sulphate solution to a bottle or measuring 
cylinder, add 10 mL of water, 15 mL of the chloroform and 10 mL of the mixed indicator solution. 
Titrate with the 0.004 M benzethonium chloride solution. Stopper the bottle or measuring cylinder after each 
addition and shake well. The lower layer will be coloured pink. Continue the titration with repeated vigorous 
shaking. As the end point approaches, the emulsions formed during shaking tend to break easily continue the DEAS 878: 2016 
© EAC 2016  – All rights reserved  23 
 titration drop by drop. Shaking after each addition of titrant, until the end point is reached. This is at the 
moment when the pink colour is completely discharged from the chloroform layer, which becomes a faint 
greyish blue. 
The molarity, M, of the benzethonium chloride solution is given by the formula: 
2125 x   VM   M
 
where, 
M1   molarity of the sodium lauryl sulphate solution; and 
V2   Volume, in mi llilitres, of benzethonium chloride added. 
F.4.5.2  Determination  
Weigh to the nearest 1 mg a sample of 30 g; dissolve the test portion in water. Add a few drops of the 
phenolphthalein solution and neutralize to a faint pink colour with the sodium hydroxide solution or sulphuric 
acid solution as required. 
Transfer to a 1-L one-mark volumetric flask and dilute to the mark with water. Mix thoroughly and, by means of 
the pipette transfer 25 mL of this solution to a bottle or measuring cylinder, , and add 10 mL of water, and 15 
mL of chloroform. Titrate with the benzethonium chloride solution as described in F.4.5.1. 
F.4.6 Expression of results 
The content as a percentage by mass, of anionic-active matter  
oo 3
 x 000  1 x 25100x  x 000  1  x  x  
MM M V
 
=4V3M 
The amount of active matter, expressed in milliequivalents per gram, 
o1 3 x  x 40
MM V
 
where,  
M0   mass, in grams, of the test portion; 
M   relative molar mass of anionic-active matter; 
M1   molarity of the benzethonium chloride solution; 
V3   volume, in millilitres, of benzethonium chloride solution used for the titration of a 25- mL aliquot of 
anionic-active matter solution.  
24 © EAC 2016  – All rights reserved  
 Annex G  
(informative)  
 
Permitted structuring and processing aids  
 
The following is the list of structuring and processing aids used generally in bathing bars.  
a) Starch and derivatives  
 
b) Cellulose and derivatives  
 
c) Propylene glycol  
 
d) Sorbitol  
 
e) Glycerol  
 
f) Dextrin  
 
g) Kaolin  
 
h) Talc  
 
i) Bentonite  
 
j) Calcite  
 
k) Sodium lactate  
 
l) Soda ash  
 
m) Vegetable/animal oil fatty acids and salts  
 
n) Phosphates  
 
o) Sodium chloride  
 
p) Sodium sulphate  
 
q) Dolomite  
 
r) Fatty alcohol  
 
s) Rosin and rosin salts  
 
t) Fatty acid ethanolamide  
 
u) Diethylene glycol monostearate  
 
v) Paraffin  
 
w) Polyoxyethylene glycol  
 
x) Glycerol monostearates  
 
y) Silicates  
 
z) Sodium citrate 
aa) Chelating agents 
 
bb) Any other internationally accepted builder DEAS 878: 2016 
© EAC 2016  – All rights reserved  25 
 Annex H  
(informative)  
 
Permitted antibacterial agents  
 
The following is the list of antibacterial agents used generally in antibacterial bathing bars.  
a) Triclosan (TCN)   
 
b) Trichlorocarbanilide (TCC) 
 
c) Zinc oxide  
 
d) Hexa chlorophene  
 
e) Chloro xylenols 
 
f) Plant extracts 
 
g) Any other internationally accepted antibacterial agent.
 
 
Bibliography 
 
      KS 2231:2012, Antibacterial bathing bars -- Specification  FDEAS 766-1: 2016    
 
© EAC 2016  – All rights reserved