Reference number  
DRS 392-2: 2018  
© RSB 2018  
 
 
 RWANDA 
STANDARD  DRS  
392-2 
 
First edition  
2018 -mm-dd 
 
Skin appli ed mosquito repellents — 
Specification  — 
Part 2: 
Spray s and roll -ons 
  DRS 392-2: 2018  
©RSB 2018 - All rights reserved                  ii 
 In order to match with technological development and to keep continuous progress in industries, 
standards are subject to periodic review. Users shall ascertain that they are in possession of the latest 
edition  
 
 
 
 
 
 
 
 
© RSB 2018  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized 
in any form or by any means, electronic or mechanical, including photocopying and microfilm, without 
prior written permission from R SB. 
Requests for permission to reprodu ce this document should be addressed to : 
Rwanda Standards  Board  
P.O Box 7099 Kigali -Rwanda  
KK 15 Rd, 49  
Tel. +250 788303492  
Toll Free: 3250  
E-mail: info@rsb.gov.rw  
Website: www.rs b.gov.rw  
ePortal: www.portal.rsb.gov.rw  DRS 392-2: 2018  
iii                ©RSB 2018 - All rights reserved  
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ....................  v 
1 Scope  ................................ ................................ ................................ ................................ .............  1 
2 Normative references  ................................ ................................ ................................ ...................  1 
3 Terms and definitions  ................................ ................................ ................................ ..................  1 
4 Requirements  ................................ ................................ ................................ ................................  3 
4.1 General  ................................ ................................ ................................ ................................ ..........  3 
4.2 Active ingredients and synergists  ................................ ................................ ..............................  4 
4.2.1  Natural repellents  ................................ ................................ ................................ .........................  4 
4.2.2  Synthetic repellents  ................................ ................................ ................................ .....................  4 
4.2.3 Synergist content  ................................ ................................ ................................ .........................  5 
4.3 Specific requirements  ................................ ................................ ................................ ..................  5 
4.4 Requirements for aerosol containers ................................ ................................ .........................  5 
4.5 Biological efficacy  ................................ ................................ ................................ ........................  6 
5 Pack aging and labelling ................................ ................................ ................................ ...............  6 
5.1 Roll ball construction  ................................ ................................ ................................ ...................  6 
5.2 Aerosol containers  ................................ ................................ ................................ .......................  6 
5.3 Labelling  ................................ ................................ ................................ ................................ ........  6 
6 Sampling  ................................ ................................ ................................ ................................ ....... 7 
Annex  A (normative)  Solvents not permitted for use in aerosols  ................................ .......................  8 
Annex  B (normative)  Determination of DEET content  ................................ ................................ ..........  9 
B.1 General  ................................ ................................ ................................ ................................ ..........  9 
B.2 Apparatus  ................................ ................................ ................................ ................................ ...... 9 
B.3 Preparation of calibration curve  ................................ ................................ ................................ . 9 
B.4 Procedure  ................................ ................................ ................................ ................................ ...... 9 
B.5 Calculation  ................................ ................................ ................................ ................................ .. 10 
Annex  C (nor mative)  Stability of smell  ................................ ................................ ................................ . 11 
C.1 Apparatus  ................................ ................................ ................................ ................................ .... 11 
C.2 Procedure  ................................ ................................ ................................ ................................ .... 11 
C.3 Result  ................................ ................................ ................................ ................................ ...........  11 
Annex  D (normative )  Non -volatile matter  ................................ ................................ .............................  12 
D.1 Apparatus  ................................ ................................ ................................ ................................ .... 12 
D.2 Procedure  ................................ ................................ ................................ ................................ .... 12 
D.3 Calculation  ................................ ................................ ................................ ................................ .. 12 
Annex  E (nor mative)  Determination of propellant composition  ................................ ........................  13 
E.1 Procedure  ................................ ................................ ................................ ................................ .... 13 
E.2 Results  ................................ ................................ ................................ ................................ .........  13 
Annex  F (normati ve)  Determination of delivery rate of the dispenser  ................................ ..............  14 
F.1 Material and apparatus  ................................ ................................ ................................ ..............  14 
F.2 Procedure  ................................ ................................ ................................ ................................ .... 14 
F.3 Calculation  ................................ ................................ ................................ ................................ .. 14 
Annex  G (nor mative)  Net weight delivery  ................................ ................................ ............................  16 DRS 392-2: 2018  
©RSB 2018 - All rights reserved                  iv 
 G.1 Procedure  ................................ ................................ ................................ ................................ ... 16 
Annex  H (normative)  Testing of filled aerosol containers  ................................ ................................ . 17 
H.1 Procedure  ................................ ................................ ................................ ................................ ... 17 
H.2 Interpretation of results  ................................ ................................ ................................ ............  17 
Annex  I (normative)  Flame propagation  ................................ ................................ ..............................  18 
I.1 Principle  ................................ ................................ ................................ ................................ ...... 18 
I.2 Apparatus  ................................ ................................ ................................ ................................ ... 18 
I.3 Procedure  ................................ ................................ ................................ ................................ ... 18 
 DRS 392-2: 2018  
v                ©RSB 2018 - All rights reserved  
 Foreword  
Rwanda Standard s are prepared  by Technical Committees and approved by R wanda Standards Board (RSB) 
Board  of Directors in  accordance with the procedures of RSB, in compliance with Annex 3 of the WTO/TBT 
agreement on the preparation, adoption and application of standards.  
The main task o f technical committees is to prepare national standards.  Final Draft Rwanda S tandards adopted 
by Technical committ ees are ratified  by me mbers of RSB Board of Director s for publication and gazettment as 
Rwanda Standards.  
DRS 392 -2 was prepared by Technical Committee RSB/TC 015, Pharmaceutical Products . 
DRS 392  consists of the following parts, under the general title Skin appli ed mosquito repellents — Specification : 
 Part 1: Lotion s, cream s, gels  and ointments  
 Part 2: Spray s and roll -ons 
Part 3: Wipes  
Part 4: Soaps 
Part 5: Bracelet s, wristbands  and patches  
Committee membership  
The following organizations were represented on the Technical Committee on Pharmaceutical Products  
(RSB/TC 015) in the preparation of this standard.  
National Industrial Research  and Development Agency (NIRDA)  
National Pharmacy Council (NPC)  
University of Rwanda/College of Sciences and Technology (UR/CST)  
Pharmacie NOVA  
Rwanda Development Board (RDB)  
AGROPY LTD  
IKIREZI NATURAL PRODUCTS  
HORIZON/SOPYRWA  
Rwanda Social Security Board (RSSB)  DRS 392-2: 2018  
©RSB 2018 - All rights reserved                  vi 
 Pharmavie  
University of Rwanda/College of Medicine and Health Sciences (UR/CMHS)  
Rwanda Biomedical Center/ Malaria and Other Parasitic Diseases Division  (RBC/MOPDD)  
Society for Family Health  (SFH) – Rwanda  
Rwanda Biomedical Center/Medical Procuremen t and Production Division (RBC/MPPD)  
INES - RUHENGERI  
Rwanda  Standards Board  (RSB) – Secretariat  DRS 392-2: 2018  
vii                ©RSB 2018 - All rights reserved  
 Introduction  
Mosquito repellents are an effective complement to bed nets in the prevention of mosquito borne diseases, 
especially malaria.   
 
Insecticides are  used either for killing or controlling harmful insects. The insecticides which are applied for 
repelling insects are termed as “Repellents". Mosquito is one of the most harmful insects for mankind. To 
destroy them, many preparations are available on the m arket in various recipes like pest killer spray, soap, oil, 
powder, repellent etc. Out of these, mosquito repellent is the most popular as it has germicidal and 
disinfectant properties and is able to repel mosquitoes and is convenient to use.  
 
The mosquito  repellent  is used for warding off mosquitoes which is the most harmful insect. Nowadays, 
mosquito repellents are used for controlling mosquito es and are becoming most preferably than other  mosquito 
destroyers gradually. With the rise in the standard of living, increasing urbanization and population, the demand 
of mosquito repellent mat is constantly increasing particularly in tropical places. It is a convenient method for 
protection against  mosquito es, so it has a tremendous market potential. Thus , there is a very good scope for 
development of such units in the country . 
 
Skin applied mosquito repellents possess the potential to target residual transmission. Entomological evidence 
has shown t hat repellents provide personal protection against malaria.  Product examples that are c ommon ly 
used  include lotions, gels, creams  and ointments , among others . However, many more  types of skin applied 
repellent products are now developed  in various forms s uch as sprays and roll -ons, wipes, soaps and bracelets , 
wristbands and patches . 
 
 
 DRS 392-2: 2018  
1                ©RSB 2018 - All rights reserved  
 Skin applied mosquito repellents — Specification — Part 2: Spray s and 
roll-ons 
1 Scope  
This Draft Rwanda S tandard prescribes the requirements, sampling and test methods for skin applied mosquito 
repellents formulated and/or prepared as spray s and roll-ons and meant to be  applied directly to skin or to 
clothing and/or footwear.  
2 Normative references  
The following documents are referred to in the text in such a way that some or all of  their content constitutes 
requirements of this document.  For dated references, only the edi tion cited applies. For undated references, the 
latest edition of the referenced document (including any amendments) applies.  
RS EAS 846, Glossary of terms relating to the cosmetic industry  
RS EAS 377 (all parts), Cosmetics and cosmetic products  
RS 191,  Refined pyrethrum concentrate ― Specification  
RS EAS 847 -17, Cosmetics — Analytical methods — Part 17: Determination of pH  
RS EAS 847 -16, Cosmetics — Analytical methods — Part 16: Determination of lead, mercury and arsenic 
content  
CIPAC 760, Determination of picaridin  
CIPAC 667, Determination of ethyl butylacetamidopropionate  
AOAC 986.03 , Determination of permethrin in pesticide formulations  
AOAC 997.07, N-octyl Bicycloheptene dicarboximide (MGK 264), Pyrethrins and Piperonyl Butoxide (PB) in 
Technical mate rials, concentrates and Finished Products.  
 
DRS 394-1, Mosquito repellents ― Performance Test Guidelines ― Part 1: Skin applied  repellents  
 
RS 91 , Labelling and marking of pharmaceutical products — Specification  
 
RS ISO 24153, Random sampling and randomization procedures  
3 Terms and definitions  
For the purposes of this standard, the terms and definitions given in RS EAS 846 and  the following apply.   
©RSB 2018 - All rights reserved                  2 
 3.1 
mosquito  
any of numerous arthropod animals of the class mosquito, having an adult stage characterized by three pairs 
of legs and a body segmented into head, thorax, and abdomen and usually having one or two pairs of wings. 
Mosquito include the flies, crickets, insects, beetles, butterflies and bees.  
3.2 
mosquito repellent  
substance applied to  skin, clothing, or other surfaces which discourages mosquito  (and arthropods  in general) 
from landing or climbing on th at surface.  
3.3 
natural repellents/biopesticides  
repellents that contain natural, plant -based compounds  or other products approved by a competent authority  
3.4 
synthetic repellents  
conventional repellents containing synthetic chemical compounds  
3.5 
roll-on 
cosmetic preparation with the effect of deodorizing and providing antiperspirant properties to the body of the 
user. It is packed in a container fitted with a roll -ball. 
3.6 
roll-ball 
spherically shaped object, with the capacity to roll in all directions.  It is put at the opening of a roll -on container 
and serves the role of closing the container as well as dispensing the contents, when rolled on the skin  
3.7 
DEET   
N,N-Diethyl -meta -toluamide  or diethyltoluamide  
3.8 DRS 392-2: 2018  
3                ©RSB 2018 - All rights reserved  
 IR3535  
ethyl butylacetylaminopropionate  
3.9 
Picaridin  
1-(1-methylpropoxycarbonyl) -2-(2-hydroxyethyl) piperidine or 2 -(2-hydroxyethyl) -1-piperidinecarboxylic acid 1 -
methylpropyl ester  
3.10 
MGK 264  
 
4-(2-Ethyl -hexyl) -4-aza-tricyclo[5.2.1.02,6]dec-8-ene-3,5-dione  
 
3.7 
PBO   
Piperonyl butoxide  
4 Requir ements  
4.1 General  
4.1.1  The repellent shall contain required amounts of act ive ingredients and when applied to the skin shall have 
the effect of repelling insects . The repellent shall be in the form of:  
a) Sprays : This shall consist of a liquid formulation in a pressurised, non -refillable aerosol dispenser, 
containing active ingredient (s), and/or synergist(s) and other formulants  including propellants and 
solvents , 
b) Roll-ons: The product may also be in form of cosmetic preparation meant to be applied as  a roll -on, and 
which contains  active ingredient(s), synergist(s) and /or other formulants  
5.1.2 The formulation  shall be of uniform colour and shall be free from visible impurities.  
5.1.3 The aerosols shall not contain s olvents and propellants listed in annex A . 
5.1.4  All ingredients shall meet the requirements of RS EAS 377.  
5.1.5 When applied to the skin, clothes or shoes, the product shall have the benefit of repelling mosquitoes  
and shall not have a harmful effect to the skin .  
©RSB 2018 - All rights reserved                  4 
 4.2 Active ingredients and synergist s 
4.2.1  Natural repellents  
4.2.1.1  Active ingredients used in natural repellents shall be natural plant based active ingredients such as 
essential oils or any other  plant extract approved as mosquito repellents.  
4.2.1.2 The manufacturer  shall provide adequate data on the repellence of such ingredients.  
4.2.1.3 The manufacturer  shall have adequate data justifying the proportion of ingredient(s) for which claims 
are made, used in the product.  
4.2.1.4 The essential oils  and other plant extracts  used in natural repellents shall be, but not limited to:  
a) Cedarwood oil ; 
b) Tea tree oil ; 
c) Geranium oil ; 
d) Rosemary oil ; 
e) Lemongrass oil ; 
f) Citronella oil ; 
g) Eucalyptus oil ; and  
h) Cinnamon oil  
i) Neem oil  
4.1.2.6  The proportion of single or blend ed active ingredient (s)  in natural repellent shall be set by the 
manufacturer in accordance with specific standard  and records shall be availed.  
4.1.2.7  Pyrethrum extracts such as pyrethrin s shall be considered in natural repellents. The limits of pyrethr ins 
in natural repellents shall not be less than 0.5 % and the extract used shall meet the requirements of RS 191.  
4.2.2  Synthetic repellents  
4.2.2.1  Synthetic repellents shall contain synthetic chemical compounds which are able to discourage 
mosquitoes and send them flying or crawling  away.  
4.2.2.2  If a synthetic active ingredient  is blended with other active ingredient (s), either natural or synthetic , 
the proportion shall be set by the manufacturer based on scientific research and records shall be availed.  DRS 392-2: 2018  
5                ©RSB 2018 - All rights reserved  
 4.2.2.3  Active ingredients and their content in synthetic repellents shall meet the requirements prescribed in 
table 1.  
Table 1 – Active ingredient s for synthetic repellents  
S/N Active ingredient  Limits  (%, w/w)  Identification 
method  
1 DEET  4 –50 Annex B  
2 Picaridin   5 – 20 CIPAC 740  
3 IR3535  7.5 – 20 CIPAC 667  
4 Permethrina 0.5 AOAC 986.03  
a. Permerthrin shall be applied to clothing , footwear  bed nets, and camping gear , but not 
directly to skin  or to inner clothing (socks or underwear) . 
4.2.2.4  Synthetic repellents and their active ingredients shall be approved and registered by competent 
authority  before being released to the market . 
4.2.3  Synergist content  
4.2.3.1 The synergist content shall be declared and, when determined, the average content measured shall 
not differ from that declared . 
4.2.3.2  The synergist shall be PBO, sesame seed oil (sesamin, sesamolin) and/or MGK 264.  The synergis t 
shall be tested in  accordance with AOAC 997.07 . The ratio of the active ingredient to the synergist shall be 
indicated and records availed.  
4.2.3.3 The ratio of the active ingredient to the synergist shall be at the ratio range of 1 to 10 parts exponent’s 
active ingredient to 1 part of an insecticide’s active ingredient.  
4.3 Specific  requirements  
The product shall comply with the quality requirements given in table 2. 
Table 2 — Specific  requirements  
S/N Parameters  Requirements  Test methods  
i.  pH  3 – 7 RS EAS 847 -17 
ii.  Non-volatile matter, % m/m, min.  10 Annex D  
iii.  Lead  (Pb) , , mg/kg, max  20 RS EAS 847 -16 
iv.  Arsenic ( As), mg/kg, max  2 
v.  Mercure ( Hg), mg/kg, max  2 
 
4.4 Requirements for aerosol containers  
The product pack aged in aerosol containers shall meet the requirements given in table 3.  
©RSB 2018 - All rights reserved                  6 
 Table 3 — Specific requirements for aerosol containers  
S/N Parameters  Requirements  Test method  
i.  CFCs  Absent  Annex E  
ii.  Delivery rate g/s, min.  0.01 Annex F  
iii.  Net weight delivery, % m/m,  min 95 Annex G  
iv.  General leakage  To pass test  Annex H  
4.5 Biological efficacy  
When tested in accordance with DRS 394 -1, the product shall repel 100 % of the mosquitoes from landing or 
climbing on that surface, within  protection time indicated by the manufacturer.  
5 Packaging and labelling  
The product shall be packaged as roll -ons or aerosols products and in any other suitable containers that shall 
protect the contents and shall not react with the product  or cause any contamination during storage, handli ng 
or use.  
5.1 Roll ball construction  
5.1.1 If the container is fitted with a roll -on: 
5.1.1.1  The roll ball shall be made of plastic material.  
5.1.1.2  The roll ball shall be fitting on the container such that on holding the container inside down the 
contents shall not pour out.  
5.1.1.3  The roll ball shall be free rolling, leaving a thin layer of the contents on the skin during dispensation . 
5.2 Aerosol containers  
Filled aerosol containers shall be appropriately  classified in  terms of flame propagation characteristics of their 
contents when tested in accordance with annex I. 
Highly flammable  — if the average length of the flame is greater than 0.45 m or if the flame burns back to the 
actuator, or continues to burn when the test  flame is extinguished.  
Flammable  — if the average length of the flame is between 0.20 m and 0.45 m  
Non-flammable  — if the product does not burn in the manner described above (a) and (b)  
5.3 Labelling  
The containers shall be securely closed and in addition to the labelling requirements of RS 91 , the following 
information shall be indelibly and legibly marked on the container:  DRS 392-2: 2018  
7                ©RSB 2018 - All rights reserved  
 a) name of the product;  
b) name and full address of the manufacture;  
c) batch number;  
d) date of manufacture and expiry;  
f) quantity in the container ; 
g) active ingredient content, percent (%,m/m);  
h) list of other ingredients  
i) net mass of content;  
j) directions for use;  
k) safety precaution ; 
l) special person s whose exposure is prohibited ( out of reach of children  and pregnant women) ; and 
m) storage conditions . 
6 Sampling  
Random samples of the product shall be drawn for test in accordance with RS ISO 24153 from the market, 
factory or anywhere else . 
  
©RSB 2018 - All rights reserved                  8 
 Annex  A 
(normative)  
 
Solvents not permitted for use in aerosols  
1) Benzene  
2) 2-butoxyethanol (ethylene glycol monobutyl ether)  
3) 2-butoxyethylacetate (ethylene glycol monobutyl ether acetate)  
4) carbon tetrachloride  
5) chlorobenzene  
6) chloroform  
7) 1,2-dichloroethane (ethylene dichloride)  
8) 2-ethoxyethanol (ethylene glycol monoethyl ether)  
9) 2-ethoxyethylacetate ( ethylene glycol monoethyl ether acetate)  
10) n-hexane  
11) 2-hexanone (methyl n -butyl ketone)  
12) 2-methoxyethanol (ethylene glycol monomethyl ether)  
13) 2-methoxyethylacetate (ethylene glycol monomethyl ether acetate)  
14) tetrachloroethylene  
15) trichloroethylene  
16) Propellants  
NOTE  The Montreal Protocol and EU 1 directive on the withdrawal of chlorofluorocarbons (CFCs) from aerosols were 
noted. Hydrocarbon propellants are recommended  for insecticide aerosols, provided international safety standards are met 
by the aerosol producer. In dustry should be encouraged to develop alternative and safer propellants and delivery systems.  
  DRS 392-2: 2018  
9                ©RSB 2018 - All rights reserved  
 Annex  B 
(normative)  
 
Determination of DEET content  
B.1 General  
The sample is dissolved in carbon disulfide and the difference in absorbance at 14.18 µm and at 14.48 µm is  
determined. The quantity of meta -isomer is obtained from this value by means of a calibration curve prepared 
by the use of a reference standard.  
B.2 Apparatus  
B.2.1  Double -beam infrared spectrophotometer. Perkin -Elmer model 21 or equivalent.  
B.2.2  Two equival ent infrared absorption cells, with sodium chloride windows and a path length of 
approximately 0.4 mm.  
B.3 Preparation of calibration curve  
B.3.1 Weigh (to the nearest 0.1 mg) into four volumetric flasks sufficient amounts of the reference DEET 
standard of known  purity to give concentrations of approximately 20, 40, 60 and 80 g/L when dissolved in carbon 
disulfide.  
B.3.2  Fill the reference cell with carbon disulfide and the sample cell with each of the standard solutions in 
turn, and record the spectra. The spectrum may be scanned rapidly, except for the region 12 – 15 µm, where a 
normal speed should be used. Carry out a blank measurement with carbon disulfide to correct for any inequality 
in the paired cells and to determine whether a cell correction is requ ired. 
B.3.3 Measure the absorbance at 14.18 µm and at 14.48 µm and calculate the difference between these 
value s, ΔA, for each of the solutions. Plot the values of ΔA against the concentration (g/l) of the meta -isomer.  
 
B.3.4  If a cell correction is requir ed, the value of ΔA is determined from the formula:  
 
  ΔA = [A 14.18 – A14.48]ref. - [A14.48]blank 
 
  Where ref. = determination with reference standard  
    blank = determination on CS 2 blank  
 
B.4 Procedure  
Weigh (to the nearest 0.1 mg) about 0.5 g of the sample, transfer quantitatively to a 10 mL volumetric flask, 
and make up to the mark with carbon disulfide. Measure the infrared absorption at 14.18 μm and 14.48 μm 
using the same conditions as described in section A.3. Determine the con centration of meta -isomer by 
comparing this value with the calibration curve. A standard sample should be run each day to check the 
calibration of the instrument.   
©RSB 2018 - All rights reserved                  10 
 B.5 Calculation  
DEET content (g/kg) =𝐶1 𝑥 𝑃 
𝐶2 
Where,  
 C1 = concentration (g/L) of standard D EET found from calibration curve  
 C2 = concentration (g/L) of sample taken  
   P = purity (g/kg) of the reference standard.  DRS 392-2: 2018  
11                ©RSB 2018 - All rights reserved  
 Annex  C 
(normative)  
 
Stability of smell  
C.1 Apparatus  
C.1.1 Porcelain cup  
C.1.2 Pincers  
C.1.3 Ten pieces of bleached gauze of dimension 5 cm x 10 cm  
C.1.4 Thermometer  
C.1.5 Hygrometer  
C.2 Procedure  
Put some pieces of bleached gauze which have been pre -washed in hot water without soap and dried into a 
porcelain cup and pour 1.5 ml of the sample into this cup. After the gauze gets soaked, take it out with the help 
of pincers. Without squeezing it, dry it in a premise having temperature 37 °C ± 2 °C and humidity of 65 % ± 
5 % for 12 h.  
C.3 Result  
The product shall be taken to have passed the test if, after 12 h, the smell of the sample can clearly be picked  
up.  
©RSB 2018 - All rights reserved                  12 
 Annex  D 
(normative)  
 
Non-volatile matter  
D.1 Apparatus  
D.1.1   Moisture dish  
D.1.2  Oven  
D.1.3  Analytical balance  
D.1.4  Desiccator  
D.2 Procedure  
Weigh accurately 1g ± 0.2 g of the sample in the dish and place it in an oven at 105 °C ± 2°C for 1 h. Cool to 
room tempera ture in a desiccator and weigh the dish. Repeat the process to bring it to constant mass.  
D.3 Calculation  
Non-volatile matter per cent by mass = =M2−M1
𝑀 𝑥 100 
Where,  
M = mass, in grams of the material taken;  
M1 = mass in grams of the dry and empty dish, and  
M2= mass in grams of the dish and dried material.  DRS 392-2: 2018  
13                ©RSB 2018 - All rights reserved  
 Annex  E 
(normative)  
 
Determination of propell ant composition  
E.1 Procedure  
E.1.1  The analysis of the propellant mixture in most aerosols  is carried out conveniently by gas 
chromatography. For Sampling, a hypodermic ne edle is fitted to the valve of the aerosol can and approximately 
0.5 g of the propellant is injected into the heavy duty centrifuge tube closed with serum cap, containing about 8 
ml of benzene. After mixing, 5μl samples are taken out from this tube with a microlitre syringe and injected into 
the gas chromatograph.  
E.1.2  Two 4572 mm × 6.35 mm OD columns operated at 40 °C are recommended for the analysis containing 
20 percent weight hexadecane and diethylhexyl sebacate respectively on silanized chromosorb W60 /S0 mesh.  
E.1.3  The first column should be used mainly for initial screening and the second column for the 
confirmation and determination of the identified propellants.  
E.1.4  Table C1 lists the relative retention data of the most widely used propellant together with some other 
fluorinate hydrocarbons and benzene used as the solvent IN the two columns.  
Table C1 – Relative retention data of propellants  
Chemical name  Stationary phase 
diethylhexyl sebacate  Stationary phase hexadecane  
Octafluorocylobutane  0.214  0.122  
1-chloro -1,2,2 trifluoroethylene  0.268  0.196  
Propane  0.275  0.22 
1,2-difluoroethane  0.289  0.141  
Dichlorodifluoromethane  0.296  0.220  
1,2-dichloro1,1.2,2 -tetrafluoromethane  0.345  0.290  
Isobutane  0.366  0.378  
Monochlorodiflouoromethane  0.368  0.152  
1-Chloro -1,1-difluoroethane  0.402  0.236  
n- butane  0.449  0.527  
Vinylchloride  0.529  0.353  
Trichlorofluoroethane  1.000  1.000  
1,1,2 -trichloro -1,2,2 -tetrafluoroethane  1.254  1.342  
Dichloromonofluoroethane  1.354  0.515  
1,2-dibromo -1,1,2,2 tetrafluoroethane  1.634  1.363  
Methylene Chloride  2.565  1.070  
Benzene  6.786  5.661  
 
E.2 Results  
The sample shall be considered as having failed the test if it contains any of the above CFCs   
©RSB 2018 - All rights reserved                  14 
 Annex  F 
(normative)  
 
Determination of delivery rate of the dispenser  
F.1 Material and apparatus  
The following material and apparatus shall be used in this test  
F.1.1  Any suitable timing device 
F.1.2  Balance  
Having accuracy to 0.01 g and with a capacity greater than 500 g.  
F.1.3  Pair of gloves  
Made of cloth or fabric or towel for handling dispensers during test.  
F.1.3  Pair of tongs  
For removing dispensers from water bath.  
F.1.3  Water bath  
Set at 26 °C ± 0.3 °C, thermostatically controlled.  
F.2 Procedure  
F.2.1  Hold a dispenser upright, spray for two seconds to fill t he eduction tube. Then weigh the dispenser  
F.2.2  Submerge the dispenser into the water bath for 15 minutes using tongs, remove the dispenser from 
the bath and immediately dry the container with a towel Spray the dispenser in one continuous burst for 10 
seconds. Re -weigh the dispenser.  
F.2.3  Repeat the procedure and take an average of three tests. The difference between the maximum and 
minimum delivery rates shall not exceed 0.2 g per second . 
F.3 Calculation  
Calculate the delivery rate according to the following  formula;  DRS 392-2: 2018  
15                ©RSB 2018 - All rights reserved  
  Delivery rate (in g per second) = 𝑀1−𝑀3
𝑁 
where;  
M1 = initial weight of the dispenser in grams  
M2 = final weight of the dispenser in grams  
N = time in seconds   
©RSB 2018 - All rights reserved                  16 
 Annex  G 
(normative)  
 
Net weight delivery  
G.1 Procedure  
G.1.1  For the determination of the net weight delivery, a random sample of at least three packages is selected. 
After the removal of any dust cover or caps not required for dispensing the product, the gross weight of each 
package is determined and after shaking for 15 sec, the content of the li ghtest container is drained by holding 
the valve wide open. Now the exhausted container is weighed .The result is called wet –tare weight and is equal 
to the weight of the container plus any product remaining after draining.  
G.1.2  Consequently, the regeneration allowance is determined and subtracted from the wet -tare weight to 
obtain the corrected wet -tare weight. The regeneration allowance is defined as the difference between the 
weight of the product which would be delivered through normal usage an d the weight of the product delivered 
by the present accelerated procedure.It is calculated by multiplying the label weight of the container by 0.02 g 
and rounding the result to the next lowest gram.  
G.1.3  By subtracting the corrected tare weight from the gross weight, the adjusted net weight of the package 
is obtained. If this is greater than 95 % of the label weight the lot is assumed to be satisfactory. However, if it is 
less than 95 % of the label weight, the lot is rejected.  DRS 392-2: 2018  
17                ©RSB 2018 - All rights reserved  
 Annex  H 
(normative)  
 
Testing of fi lled aerosol containers  
H.1 Procedure  
H.1.1  All filled aerosol containers shall be tested by immersion in a water bath set at 55 °C . 
H.1.2  The container shall be such that the pressure generated within the immersed container reaches not 
less than 90 percent of  the pressure generated within the containers at equilibrium at 55 °C.  
H.2 Interpretation of results  
Any filled aerosol container that shall leak, get distorted or burst as a result of this test shall be considered to 
have failed the test and shall be discarde d.  
©RSB 2018 - All rights reserved                  18 
 Annex  I 
(normative)  
 
Flame propagation  
I.1 Principle  
The filled aerosol container is sprayed as a test flame under controlled conditions and length of the burning spry 
cone is measured.  
I.2 Apparatus  
I.2.1 In its simplest form, the apparatus consists of a base marked at 0.15 m intervals, an adjustable stand to 
carry the aerosol container which may be raised or lowered to accommodate differences in container height, a 
means of measuring the burning spray cone (usually a one metre fuel placed horizontally at the same lev el as 
the top third of the flame, the hottest part) a means of igniting the spray cone in the form of a test flame 0.05 m 
± 0.005 m in height (usually a candle flame is used).  
I.2.2  Water bath maintained at 20 °C .This equipment shall be used to bring the  aerosol container and its 
contents to equilibrium at 20 °C (Heat the cans to 20 °C in the water bath) . 
I.3 Procedure  
I.3.1 Place the aerosol container on the stand. Depress the actuator and adjust the height of the stand so that 
the spray cone will pass throu gh the upper third test flame (hottest part).  
I.3.2  Bring the aerosol container and its content to the equilibrium temperature of 20 °C. Place the 
container on the stand so that the point where the spray emerges is 0.15 m from the test flame. Then light the 
test flame and depress the actuator for 15 seconds to 20 seconds. Record the total length of the burning spray 
cone and specify whether or not it burns back to the actuator.  
 
I.3.3  Extinguish the test flame and record whether the spray cone continues to burn while the actuator is 
depressed.  
I.3.4  Repeat each test twice and record the flame length as the average of the three tests.  DRS 392-2: 2018  
19                ©RSB 2018 - All rights reserved  
 Bibliography  
[1] USPC/USP Pharmacopeial Forum 2009 (Vol. 35 (3),  Topical and Transdermal Drug Products  
[2] Manual on development and use of FAO and WHO specifications for pesticides, November 2010, 2nd 
Edition.  
[3] Product Performance Test Guidelines. OPPTS 810.3700 : Insect Repellents to be applied to human Skin  
[4] Official Methods of Analysis of AOAC International, 19th Edition, 2012, volume I  DRS 392-2: 2018  
ICS 65.100  
 
 
©RSB 2018 - All right s reserved