DRAFT UGANDA STANDARD ON PUBLIC REVIEW FINAL DRAFT UGANDA 
STANDARD  
  FDUS EAS 820 
First  Edition  
2015-mm-dd 
 
 Reference number  
FDUS EAS 820: 2014 
© UNBS 201 5 
  
Dextrose monohydrate (glucose powder) — Specification  
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDUS EAS 820: 2014 
ii                © UNBS  2015 - All rights reserved 
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2015 
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS. 
Requests for permission to reproduce this document should be addressed to 
The Executive Director 
Uganda National Bureau of Standards 
P.O. Box 6329 
Kampala 
Uganda 
Tel: 256 41 505 995 
Fax: 256 41 286 123 
E-mail: unbs@infocom.co.ug  
Web: www.unbs.go.ug  
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDUS EAS 820: 2014 
© UNBS  2015 - All rights reserved                iii National foreword 
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade, Industry and 
Cooperatives established under Cap 327, of the Laws of Uganda, as amended.  UNBS is mandated to co-
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT Agreement of the World Trade Organisation (WTO). 
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of representatives of 
consumers, traders, academicians, manufacturers, government and other stakeholders. 
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
This Final Draft Uganda Standard, FDUS EAS 8 20: 2014 , Dextrose monohydrate (glucose powder) — 
Specification , is identical with  and has been reproduced from an East Afri can Standard , EAS 820: 2014, 
Dextrose monohydrate (glucose powder) — Specification , and adopted  as a Uganda Standard.  
Wherever the words, “ East African Standard" appear, they should be replaced by "Uganda Standard." 
 
  DRAFT UGANDA STANDARD ON PUBLIC REVIEW FDEAS 820: 2014 
ICS 67.180.10 
© EAC 2014                                                                                                                              First Edition 2014  
 
FINAL DRAFT EAST AFRICAN STANDARD 
Dextrose monohydrate (glucose powder) — Specification 
EAST AFRICAN COMMUNITY DRAFT UGANDA STANDARD ON PUBLIC REVIEWDRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 820: 2014  
ii © EAC  2014  – All rights reserved  
 Copyright notice 
This EAC document is copyright-protected by EAC. While the reproduction of this document by 
participants in the EAC standards development process is permitted without prior permission from EAC, 
neither this document nor any extract from it may be reproduced, stored or transmitted in any form for 
any other purpose without prior written permission from EAC. 
Requests for permission to reproduce this document for the purpose of selling it should be addressed 
as shown below or to EAC’s member body in the country of the requester:  
© East African Community 2014  — All rights reserved 
East African Community 
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Reproduction for sales purposes may be subject to royalty payments or a licensing agreement. 
Violators may be persecuted DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEA S 820: 2014  
© EAC 2014  – All rights reserved  iii 
 Foreword 
Development of the East African Standards has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
In order to achieve this objective, the Community established an East African Standards Committee mandated 
to develop and issue East African Standards.   
The Committee is composed of representatives of the National Standards Bodies in Partner States, together 
with the representatives from the private sectors and consumer organizations. Draft East African Standards 
are circulated to stakeholders through the National Standards Bodies in the Partner States. The comments 
received are discussed and incorporated before finalization of standards, in accordance with the procedures 
of the Community. 
East African Standards are subject to review, to keep pace with technological advances. Users of the Eas t 
African Standards are therefore expected to ensure that they always have the latest versions of the standards 
they are implementing .  
FDEAS 820 was prepared by Technical Committee EASC/TC 19, Sugar and sugar confectionary . 
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWDRAFT UGANDA STANDARD ON PUBLIC REVIEWFINAL DRAFT EAST AFRICAN STANDARD  FDEAS 820: 2014  
 
© EAC 2014  – All rights reserved  1 
 Dextrose monohydrate (glocuse powder) — Specification 
1 Scope 
1.1 This Final draft East African Standard specifies the requirements and methods of sampling and test for 
dextrose monohydrate (glucose powder) intended for human consumption as food and industrial applications.  
1.2 This standard does not apply to dextrose monohydrate for intravenous applications. 
2 Normative references 
The following referenced documents are indispensable for the application of this document. For dated 
references, only the edition cited applies. For undated references, the latest edition of the referenced 
document (including any amendments) applies. 
EAS 39, Hygiene for food and drink manufacturing industry — Code of practice  
EAS 38, Labelling of pre-packaged foods — Specification 
ISO 1741, Dextrose — Determination of loss in mass on drying — Vacuum oven method 
ISO 1743, Glucose syrup —-- Determination of dry matter content — Refractive index method 
ISO 4832 , Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of 
coliforms — Colony-count technique  
ISO 4833- 1, Microbiology food chain — Horizontal method for enumeration for microorganism — Part 1, 
colony count at 30 degrees C by the pour plate technique  
ISO 5377, Starch hydrolysis products — Determination of reducing power and dextrose equivalent — Lane 
and Eynon constant titre method 
ISO 5379, Starches and derived products — Determination of sulphur dioxide content — Acidimetric method 
and nephelometric method  
ISO 5809 , Starches and derived products — Determination of sulphated ash 
ISO 6579, Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella 
spp 
ISO 6888 1, Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of 
coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using 
Baird-Parker agar medium 
ISO 7251, Microbiology of food and animal feeding stuffs- Horizontal method for detection and enumeration of 
presumptive Escherichia Coli - Most probable number technique 
ISO 21527- 2, Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of 
yeasts and moulds -- part 2 colony count technique in products with water activity less than or equal to 0.95. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 820: 2014  
2 © EAC  2014  – All rights reserved  
 3 Terms and definitions 
For the purposes of this standard, the following terms and definitions shall apply. 
3.1 
dextrose monohydrate 
purified and crystallized D-glucose containing one molecule of water of crystallization 
3.2 
extraneous matter  
organic matter originating from sugar cane plants and/or their products other than molasses 
3.3 
foreign matter 
organic and inorganic materials ( such as sand, soil, glass) and other extraneous matter in molasses 
4 Requirements 
4.1 General requirements 
Glucose powder shall be: 
a) in the form of fine white crystals or a white powder; 
b) free from off-flavours; 
c) free from foreign matter; 
d) free from other sugars, soluble starch and dextrin; and 
e) readily soluble in water and give a sweet solution.  
4.2 Specific quality requirements 
Dextrose monohydrate shall comply with the specific quality requirements specified in Table 1. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEA S 820: 2014  
© EAC 2014  – All rights reserved  3 
 Table 1 —  Specific quality requirements for dextrose monohydrate (glucose powder) 
S/N Characteristics  Requirements  Test methods  
i) Moisture content, %, max.  10 ISO 1741  
ii) Dextrose equivalent (dry matter basis), 
min. 99.5 ISO 5377  
iii) D-glucose content (dry matter basis),  %, 
m/m,  min. 99.5 ISO 5377  
iv) Sulphated ash, dry matter basis, %, m/m, 
max.  0.25 ISO 5809  
v) Sulphur dioxide (SO 2) mg/kg, max.  15 ISO 5379  
vi) Total soli ds content %, m/m, min.  90 ISO 1743  
vii) pH 4.0 – 6.0 Annex A  
viii) Total acidity as HCl/g, max.  0.05 Annex B  
 
5 Food additives 
Only the food additives permitted in CODEX STAN 192 may be used. 
6 Contaminants 
6.1 Pesticide residues 
Dextrose Monohydrate (glucose powder) shall comply with maximum pesticide residues limits established by 
the Codex Alimentarius Commission for this commodity . 
6.2 Heavy metals  
Dextrose Monohydrate (glucose powder) shall be free from heavy metals in amounts which may represent a 
hazard to human health. 
7 Hygiene 
Dextrose monohydrate  shall be prepared and handled in accordance to the EAS 39 and shall comply with 
microbiological limits specified in Table 2. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 820: 2014  
4 © EAC  2014  – All rights reserved  
 Table 2 — Microbiological limits 
S/N Parameter  Limits  Test method  
i) Yeasts and Moulds cfu per g, max.  20 ISO 21527 - 2 
ii) Coliforms cfu  per g, max  10 ISO 4832  
iii) E. Coli cfu per 30 g , max Absent  ISO 16654  
iv) Salmonella  cfu per 30 g, max.  Absent  ISO 6579  
v) Staphylococcus aureus  cfu per 30 g, max.  Absent  ISO 6888 - 1 
 
8 Packaging 
8.1 The Dextrose monohydrate shall be packed in food grade materials that ensure product safety and 
integrity. 
8.2 The fill of dextrose monohydrate shall comply with Weights and Measures regulations of Partner States 
or equivalent legislation. 
9 Labelling 
Labelling shall be done in accordance with EAS 38. In addition, the following shall be legibly and indelibly 
marked on each package: 
a) name of the product as “dextrose Monohydrate” or “Glucose powder” ; 
b) name, address and the physical location of the manufacturer/importer/packer; 
c) brand/trade name; 
d) batch or code number; 
e) net content in SI units ; 
f) storage conditions; 
g) date of manufacture; 
h) expiry date; 
i) instructions for use; 
j) country of origin; 
k) instructions on disposal of used packaged; and 
l) declaration of status if raw material is genetically modified. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEA S 820: 2014  
© EAC 2014  – All rights reserved  5 
 10 Sampling 
In drawing, preparing, storing and handling of samples, the following precautions and directions shall be 
observed. 
a) samples shall be taken in a protected place not exposed to damp air, dust or soot; 
b) the sampling instruments shall be clean and dry before use; 
c) when sampling for microbiological purposes ,the sampling instruments and containers for samples 
shall be sterilized preferably by dry heat at 170 °C for one hour before use; 
d) precautions shall be taken to protect the samples, the material being sampled, the sampling 
instruments and the containers for samples from adventitious contamination; 
e) the samples shall be placed in clean, dry, and moisture-proof containers; 
f) the sample containers shall be sealed air tight after filling, and marked with full details of sampling: the 
date of sampling, batch number, name and place of factory, name of the person sampling and other 
important particulars of the consignments; and 
g) samples shall be protected from light as far as practicable and shall be stored in a cool and dry place. 
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 820: 2014  
6 © EAC  2014  – All rights reserved  
 Annex A 
(normative)  
 
Determination of pH in dextrose monohydrate (glucose powder) 
Weigh 10 g of glucose powder and dissolve it in 100 mL of carbon dioxide-free water. Take the reading of the 
solution (pH) using a pH meter. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEA S 820: 2014  
© EAC 2014  – All rights reserved  7 
 Annex B  
(normative) 
 
Determination of total acidity in dextrose monohydrate (glucose powder) 
B.1 Procedure 
Dissolve 5.0 g of glucose powder in 50 mL of carbon dioxide-free water. Add a few drops of phenolphthalein 
indicator and titrate with 0.020 N sodium hydroxide to a distinct pink colour. Not more than 0.30 mL is required 
for neutralization. 
B.2 Calculation 
Calculate the total acidity as HCl/g. 
Total acidity = 
(g)  sample  the of WeightNormality  x  used  Titre  
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 820: 2014  
8 © EAC  2014  – All rights reserved  
 Bibliography 
[1] Sri Lanka Standard  617 Specification for glucose . 
[2] Codex Alimentarius Commission Codex Stan , Dextrose monohydrate . DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 820: 2014    
 
© EAC 2014 – All rights reserved