DRAFT UGANDA STANDARD ON PUBLIC REVIEW FINAL DRAFT UGANDA 
STANDARD  
  FDUS EAS 349 
First  Edition  
2015-mm-dd 
 
 Reference number  
FDUS EAS 349: 2014 
© UNBS 201 5 
  
Liquid glucose (glucose syrup)  — Specification  
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDUS EAS 3 49: 2014 
ii                © UNBS  2015 - All rights reserved 
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2015 
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS. 
Requests for permission to reproduce this document should be addressed to 
The Executive Director 
Uganda National Bureau of Standards 
P.O. Box 6329 
Kampala 
Uganda 
Tel: 256 41 505 995 
Fax: 256 41 286 123 
E-mail: unbs@infocom.co.ug  
Web: www.unbs.go.ug  
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDUS EAS 3 49: 2014 
© UNBS  2015 - All rights reserved                iii National foreword 
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade, Industry and 
Cooperatives established under Cap 327, of the Laws of Uganda, as amended.  UNBS is mandated to co-
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT Agreement of the World Trade Organisation (WTO). 
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of representatives of 
consumers, traders, academicians, manufacturers, government and other stakeholders. 
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
This Final Draft Uganda Standard, FDUS EAS 3 49: 2014, Liquid glucose (glucose syrup) — Specification , is 
identical with  and has been reproduced from an East African  Standard , EAS 349: 2014, Liquid glucose 
(glucose syrup)  — Specification , and adopted  as a Uganda Standard.  
Wherever the words, “ East African Standard" appear, they should be replaced by "Uganda Standard." 
 
  DRAFT UGANDA STANDARD ON PUBLIC REVIEW FDEAS 349: 2014 
ICS 67.180.10  
© EAC 2014                                                                                                                         Second Edition 2014  
 
FINAL DRAFT EAST AFRICAN STANDARD 
Liquid glucose (glucose syrup) — Specification 
EAST AFRICAN COMMUNITY DRAFT UGANDA STANDARD ON PUBLIC REVIEWDRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 349: 2014  
ii © EAC  2014  – All rights reserved  
 Copyright notice 
This EAC document is copyright-protected by EAC. While the reproduction of this document by 
participants in the EAC standards development process is permitted without prior permission from EAC, 
neither this document nor any extract from it may be reproduced, stored or transmitted in any form for 
any other purpose without prior written permission from EAC. 
Requests for permission to reproduce this document for the purpose of selling it should be addressed 
as shown below or to EAC’s member body in the country of the requester:  
© East African Community 2014  — All rights reserved 
East African Community 
P.O.Box 1096 
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Tel: 255 27 2504253/8 
Fax: 255 27 2504481/2504255 
E-mail: eac@eachq.org 
       Web: www.eac-quality.net 
Reproduction for sales purposes may be subject to royalty payments or a licensing agreement. 
Violators may be persecuted 
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 349: 2014  
© EAC 2014  – All rights reserved  iii 
 Foreword 
Development of the East African Standards has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
In order to achieve this objective, the Community established an East African Standards Committee mandated 
to develop and issue East African Standards.   
The Committee is composed of representatives of the National Standards Bodies in Partner States, together 
with the representatives from the private sectors and consumer organizations. Draft East African Standards 
are circulated to stakeholders through the National Standards Bodies in the Partner States. The comments 
received are discussed and incorporated before finalization of standards, in accordance with the procedures 
of the Community. 
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards are therefore expected to ensure that they always have the latest versions of the standards 
they are implementing .  
FDEAS 349 was prepared by Technical Committee EASC/TC 19, Sugar and sugar confectionary . 
This second edition cancels and replaces the first edition (EAS 349: 2004), which has been technically 
revised. 
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWDRAFT UGANDA STANDARD ON PUBLIC REVIEWFINAL DRAFT EAST AFRICAN STANDARD  FDEAS 349: 2014  
 
© EAC 2014  – All rights reserved  1 
 Liquid glucose (glucose syrup) — Specification 
1 Scope 
This Final Draft East African Standard specifies the requirements and the methods of sampling and test for 
liquid glucose (glucose syrup) for human consumption . 
2 Normative references 
The following referenced documents are indispensable for the application of this document. For dated 
references, only the edition cited applies. For undated references, the latest edition of the referenced 
document (including any amendments) applies. 
CODEX STAN 192, General standard for food additives 
EAS 38, Labelling of pre-packaged foods — Specification  
EAS 39, Hygiene for food and drink manufacturing industry — Code of practice  
ICUMSA GS 1/2/3/4/7/8/9 – 23, Determination of pH by direct method 
ICUMSA GS 3/4/7/8- 11, Determination of sulphated ash in brown sugar, juice, syrup and molasses 
ISO 1743, Glucose syrup , Determination of dry matter content — Refractive index method  
ISO 4833- 1, Microbiology food chain — Horizontal method for enumeration for microorganism — Part 1, 
colony count at 30  degrees  C by the pour plate technique  
ISO 5377, Starch hydrolysis products — Determination of reducing power and dextrose equivalent — Lane 
and Eynon con stant titre method  
ISO 5379, Starches and derived product — Determination of sulphur dioxide content — Acidimetric method 
and nephelometric method  
ISO 6579, Microbiology of food and animal feeding stuffs  — Horizontal method for detection of Salmonella 
spp. 
ISO 7251, Microbiology of food and  animal feeding stuffs  — Horizontal method for detection and enumeration 
of presumptive Escherichia coli — Most probable number technique  
ISO 21527- 2, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of 
yeasts and moulds — Part 2:  Colony count technique in products with water activity less than or equal to 0.95  
3 Term and definition 
For the purposes of this standard, the following term and definition shall apply. 
liquid glucose (glucose syr up)  
purified and concentrated aqueous solution of nutritive saccharose obtained from starch DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 349: 2014  
2 © EAC  2014  – All rights reserved  
 4 Requirements 
4.1 General requirements 
The product shall be: 
a) in the form of odourless and viscous syrup with characteristic sweet taste; 
b) clear, free from fermentation, mould growth, sediment, dirt or other suspended and extraneous matter; 
c) free from objectionable matter; 
d) free from added sweetening and flavouring agents; and 
e) free from added colouring material. 
4.2 Specific requirements 
4.2.1  When a 50 % (m/v) solution of the material of regular conversion grade is tested in a Lovibond 
Tintometer in a 2.54-cm cell, the colour of the material in terms of Lovibond units shall not be deeper than: 
a) 0.1 yellow and 0.1 red within a period of 90 days from the date of manufacture; and 
b) 0.2 yellow and 0.1 red within a period of 180 days from the date of manufacture. 
4.2.2  The material shall also comply with the specific quality requirements given in Table 1.  
Table 1 — Specific quality requirements for liquid glucose 
S/N Character istic  Requirement  Test Methods  
i.  Dextrose Equivalent (DE) value, % m/m  dry 
basis, min. 20 ISO 5377  
ii.  Total solids, % m/m, min.  70 ISO 1743  
iii.  Sulphated ash , % m/m, max.  1.0 ICUMSA GS 3/4/7/8 -11 
iv.  pH 4.5 - 5.5 ICUMSA GS 1/2/3/4/7/8/9 -23 
v.  Sulphur dioxide, mg/kg, max.  400 ISO 5379  
 
5 Food additives  
Only the food additives permitted in CODEX STAN 192 may be used. 
6 Contaminants 
6.1 Pesticide residues 
Liquid glucose shall comply with the maximum pesticide residues limits established by the Codex Alimentariu s 
Commission for this commodity. 
6.2 Heavy metals 
Liquid glucose shall be free from heavy metals in amounts which may represent a hazard to human health. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 349: 2014  
© EAC 2014  – All rights reserved  3 
 7 Hygiene 
Liquid glucose shall be prepared and handled in accordance with EAS 39 and shall comply with  
microbiological limits stipulated in Table 2 . 
Table 2 — Microbiological limits for liquid glucose 
S/N Microorganisms  Maximum limit  Test methods  
i.  Total aerobic count, cfu/g, max.  100 ISO 4833 -1 
ii.  Yeast and moulds,cfu/g, max.  10 ISO 21527 -2 
iii.  Escherichia c oli, cfu/g  Absent  ISO 7251  
iv.  Salmonella, cfu/ 25 g  Absent  ISO 6579  
 
8 Packaging  
Liquid glucose shall be packaged in food grade and leak-proof containers.  
9 Weights and measures 
The volume and the fill of liquid glucose shall comply with weights and m easures regulations of Partner States 
or equivalent legislation. 
10 Labelling 
Liquid glucose shall be labelled in accordance with requirements specified in EAS 38. In addition, the following 
shall be legibly and indelibly marked on each package:  
a) name and physical address of manufacturer/importer/distributor/ packer; 
b) product name as ‘’Liquid glucose (Glucose syrup) ’’; 
c) date of manufacture; 
d) expiry date; 
e) list of ingredients in descending order; 
f) storage instructions; 
g) country of origin; 
h) batch/lot number; 
i) net content in SI units; 
j) instructions for disposal of the used package; and 
k) declaration of dextrose equivalent value. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 349: 2014  
4 © EAC  2014  – All rights reserved  
 11 Sampling 
11.1 General requirements 
In drawing, preparing, storing and handling of samples, the following precautions and directions shall be 
observed: 
a) samples shall be taken in a protected place not exposed to damp air, dust or soot; 
b) sampling instruments shall be clean and dry when used; 
c) when sampling for microbiological purposes, the sampling instruments and containers for samples 
shall be sterilized preferably by dry heat at 170 °C for 1 h before use; 
d) precautions shall be taken to protect the samples, the material being sampled, the sampling 
instruments and the containers for samples from adventitious contamination; 
e) samples shall be placed in clean, dry, and moisture-proof containers; 
f) sample containers shall be sealed air-tight after filling and marked with name of material, date of 
sampling, name of the manufacturer, name of the person sampling and such other particulars of the 
consignments; and 
g) samples shall be protected from light as far as practicable and shall be stored in a cool, dry place. 
11.2 Scale of sampling 
11.2.1  All the packages of the same size, type and style which have been manufactured and packaged 
under essentially the same conditions in a single consignment shall constitute a lot. Samples shall be tested 
separately for each lot for ascertaining the conformity of the product. 
11.2.2  The number of packages to be selected ( n) from the lot shall depend on the size ( N) of the lot and 
shall be in accordance with the formula: 
n = √N 
where 
n number of packages to be selected; and 
N size of the lot. 
11.2.3  These packages shall be selected at random from the lot; to ensure the randomness of selection a 
random number table, as agreed to between the purchaser and the supplier shall be used. In case such a 
table is not available, the following procedure shall be used: 
Starting from any package, count them as 1, 2, 3,… up to r and so on in one order, where r is equal to the 
integral part of N/n, N being the total number of packages in the lot and n the number of packages to be 
selected. Every r th package thus counted shall be separated until the requisite number of packages is 
obtained from the lot to give samples for test ; in case of packages stacked in a pyramidal shape, 
approximately equal number of packages shall be selected from all exposed sides of the lot, so as to give the 
required number of sample packages. DRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 349: 2014  
© EAC 2014  – All rights reserved  5 
 11.3 Preparation of sample 
11.3.1 Procedure 
From the top, middle and bottom portions of each of the selected packages (see 11 .2) approximately equal 
quantity of product shall be taken with the help of a suitable sampling instrument. The sample collected from 
each of the packages shall be thoroughly mixed so as to give a composite sample of 600 g. The composite 
sample thus prepared shall be divided approximately into three equal parts; one for the purchaser, one for the 
supplier, and the third for the referee and sealed air tight with particulars as given in 11.1 f). 
11.3.2 Number of tests 
The composite sample prepared as under 11.3.1 shall be tested for the characteristics as prescribed in Table 
1. 
11.3.3 Criteria for conformity 
The lot shall be declared as conforming to this specification, when the test results on various characteristics 
obtained on the composite sample satisfy the corresponding requirements. 
 DRAFT UGANDA STANDARD ON PUBLIC REVIEWDRAFT UGANDA STANDARD ON PUBLIC REVIEWFDEAS 349: 2014    
 
© EAC 2014 – All rights reserved