II 
(Non-legislative acts) 
REGUL ATIONS 
COMMISSION IMPLEMENTING REGUL ATION (EU) 2020/1497 
of 15 Oct ober 2020 
concer ning the author isation of L-methionine produced by Cor ynebacter ium glutamicum KCCM 
80 184 and Escherichia coli KCCM 80 096 as a feed additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Havi ng regar d to the Treaty on the Functioning of the European Union, 
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof, 
Whereas: 
(1) Regulation (EC) No 1831/2003 provides for the author isation of additives for use in animal nutr ition. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitted for the author isation 
of L-met hionine produced by Corynebacter ium glutamicum KCCM 80 184 and Escher ichia coli KCCM 80 096 as a feed 
additive for use in feed for all animal species. That application was accompan ied by the particulars and documents 
required under Article 7(3) of Regulation (EC) No 1831/2003. 
(3) The application concer ns the author isation of L-methionine produced by Corynebacter ium glutamicum KCCM 80 184 
and Escher ichia coli KCCM 80 096 as a feed additive for all animal species to be classif ied in the additive catego ry 
‘nutr itional additives ’. 
(4) The European Food Safet y Author ity (‘the Auth ority’) concluded in its opinion of 12 Novem ber 2019 (2) that, under 
the proposed conditions of use, L-methionine produced by Corynebacter ium glutamicum KCCM 80 184 and Escher ichia 
coli KCCM 80 096 does not have an adverse effect on animal health, human health or the environment. 
(5) The Auth ority also concluded that L-methionine produced by Corynebacter ium glutamicum KCCM 80 184 and 
Escher ichia coli KCCM 80 096 is an effective source of methionine for all animal species and that in order to be as 
efficacious in ruminants as in non-r uminant species, the additive should be prot ected against degradation in the 
rumen. 
(6) The Author ity does not consider that there is a need for specif ic requirements of post-market monit oring. It also 
verified the repor t on the method of analysis of the feed additive in feed submitte d by the Reference Laboratory set 
up by Regulation (EC) No 1831/2003. 
(7) The assessment of this additive shows that the conditions for author isation, as provid ed for in Article 5 of 
Regulation (EC) No 1831/2003, are satisfied. According ly, the use of this additive should be author ised as specified 
in the Annex to this Regulation. 
(8) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed, 
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Journal 2019;17(12):5917. EN Offi cial Jour nal of the European Union 16.10.2020                                                                                                                                         L 342/1   HAS ADOPTED THIS REGUL ATION: 
Article 1 
The substance specifi ed in the Annex, belonging to the additive categor y ‘nutr itional additives ’ and to the functional group 
‘amino acids, their salts and analogues’ is author ised as a feed additive in animal nutr ition subject to the conditions laid 
down in that Annex. 
Article 2 
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union. 
This Regulation shall be binding in its entirety and directly applicable in all Member States. 
Done at Brussels, 15 Octobe r 2020.  
For the Commission 
The President 
Ursula VON DER LEYEN     EN Offi cial Jour nal of the European Union L 342/2                                                                                                                                         16.10.2020   ANNEX 
Identification 
number of the 
additive Name of the 
holder of 
author isation Additive Composition, chemical formula, descr iption, 
analytical method. Species or 
category of 
animal Maximum age Minimum 
content Maximum 
content 
Other provisions End of period of 
author isation mg/kg of complet e feed with a 
moisture content of 12 % 
Categor y: nutr itional additiv es. Functional group: amino acids, their salts and analogues 
3c305 - L-methionine Additive composition 
Powder with a minimum of 98,5 % L- 
methionine and a maximum moisture 
conte nt of 0,5 % 
—————————————— 
Char acter isation of the active substance 
L-met hionine produced by fermentation 
with Corynebacter ium glutamicum KCCM 
80 184 and Escher ichia coli KCCM 80 096 
Chemical formula: C5H11NO 2S 
CAS Number: 63-68-3. 
—————————————— 
Analytical methods  (1) 
For the determi nation of L-met hionine in 
the feed additive: 
— Food Chemical Codex ‘L-methionine 
monograph’ (identific ation) and 
— Ion-exc hang e chromat ography 
coupled with post-column derivati­
sation and optical detection (IEC- 
VIS/FLD) – EN ISO 17 180 (quantifi­
cation) 
For the deter mination of methionine in 
premixtures 
— Ion-exc hang e chromat ography 
coupled with post-column derivati­
sation and optical detection (IEC- 
VIS/FLD) – EN ISO 17 180 and 
— Ion-exc hang e chromat ography 
coupled with post-column derivati­
sation and photome tric detection 
(IEC- VIS), Commission Regulation 
(EC) No 152/2009 (Annex III, F) All species - - - 1. L-met hionine 
may be placed on 
the market and 
used as an additive 
consisting of a 
preparation. 
2. L-met hionine 
may be used via 
water for drink­
ing. 
3. The labelling of 
the additive and 
premixtures shall 
indicate the fol­
lowing: 
‘The supplemen­
tation with L- 
methionine, in 
particular via 
water for drink­
ing, shall take into 
account all essen­
tial and condi­
tionally essential 
amino acids in or­
der to avoid im­
balances.’ 5.11.2030 EN Offi cial Jour nal of the European Union 16.10.2020                                                                                                                                         L 342/3   For the deter mination of methionine in 
compo und feed and feed mater ials: 
— Ion-exc hang e chromat ography 
coupled with post-column derivati­
sation and photome tric detection 
(IEC- VIS), Commission Regulation 
(EC) No 152/2009 (Annex III, F) 
For the deter mination of methionine in 
water: 
— Ion-exc hang e chromat ography 
coupled with post-column derivati­
sation and photome tric detection 
(IEC- VIS) 
(1) Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts   EN Offi cial Jour nal of the European Union L 342/4                                                                                                                                         16.10.2020