DRAFT UGANDA STANDARD  
 DUS 1783  
First Edition  
2017 -mm-dd 
 
 
 Reference number  
DUS 1783 : 2017 
© UNBS 2017   
  
Disposable adult absorbent (incontinence) products  — 
Specifications   
 DUS 1783 : 2017  
ii © UNBS 2017  – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are  
responsible for its correct application  
© UNBS 2017   
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, wi thout prior written 
permission from UNBS.  
Requests for permission to reproduce this document should be addressed to  
The Executive Director  
Uganda National Bureau of Standards  
P.O. Box 6329  
Kampala  
Uganda  
Tel: +256 41 7 333 2 50/1/2 
Fax: +256 41 4 286 123  
E-mail: info@unbs.go.ug  
Web: www.unbs.go.ug  DUS 1783 : 2017  
© UNBS 2017  – All rights reserved  iii 
 Contents  Page 
1 Scope  ................................ ................................ ................................ ................................ ......................  5 
2 Normative references  ................................ ................................ ................................ ............................  5 
3 Terms and definitions  ................................ ................................ ................................ ...........................  5 
4 Requirements  ................................ ................................ ................................ ................................ .........  6 
4.1 Materials  ................................ ................................ ................................ ................................ .................  6 
4.1.1  Absorbent filler  ................................ ................................ ................................ ................................ ...... 6 
4.1.2  Top Sheet (the layer which contacts adult’s skin)  ................................ ................................ .............  6 
4.1.3  Back sheet  ................................ ................................ ................................ ................................ ..............  6 
4.1.4  Fastening device (closure system)  ................................ ................................ ................................ ...... 6 
4.1.5  Breathability  ................................ ................................ ................................ ................................ ...........  7 
4.2 Key performance requirements  ................................ ................................ ................................ ...........  7 
4.3 Microbiological requirements  ................................ ................................ ................................ ..............  7 
5 Packaging and marking  ................................ ................................ ................................ ........................  7 
5.1 Packaging  ................................ ................................ ................................ ................................ ...............  7 
5.2 Marking  ................................ ................................ ................................ ................................ ...................  7 
6 Sampling  ................................ ................................ ................................ ................................ ................  8 
7 Proof of compliance  ................................ ................................ ................................ ..............................  8 
8 Acceptance criteria  ................................ ................................ ................................ ...............................  8 
Annex  A (normative)  Rate of absorption and rewet test  ................................ ................................ ...............  9 
A.1 Scope and Purpose:  ................................ ................................ ................................ ..............................  9 
A.2 Principle  ................................ ................................ ................................ ................................ .................  9 
A.3 Apparatus and materials  ................................ ................................ ................................ ......................  9 
A.4 Saline solution  ................................ ................................ ................................ ................................ ....... 9 
A.5 Saline dosage  ................................ ................................ ................................ ................................ ........  9 
A.7 Calculation of results  ................................ ................................ ................................ ..........................  11 
Annex  B (normative)  Test Method for Retention Capacity  ................................ ................................ .........  12 
A.1 EQUIPMENT  ................................ ................................ ................................ ................................ .........  12 
A.2 PROCEDURE  ................................ ................................ ................................ ................................ ........  12 
A.3 CALCULATIONS  ................................ ................................ ................................ ................................ .. 12 
Annex  C (normative)  Microbiological examination  ................................ ................................ ......................  13 
C.1 Apparatus and equipment  ................................ ................................ ................................ ..................  13 
C.2 Media and reagents  ................................ ................................ ................................ .............................  13 
C.2.1  General  ................................ ................................ ................................ ................................ .................  13 
C.2.2  Bacteriological peptone  ................................ ................................ ................................ ......................  13 
C.2.3  Plate count agar  ................................ ................................ ................................ ................................ ... 13 
C.2.4  Neutral red -bile salt peptone glucose medium  ................................ ................................ ................  13 
C.2.5  Fluid soybean -casein digest medium  ................................ ................................ ...............................  14 
C.2.6  Centrimide agar medium  ................................ ................................ ................................ ....................  14 
C.2.7  Pseudomonas agar  medium for detection of fluorescein  ................................ ...............................  14 
C.2.8  Pseudomonas agar  medium for detection of pyocyanin  ................................ ................................  15 
C.3   Preparation of Test Suspension  ................................ ................................ ................................ ....... 15 
C.4  Procedure  ................................ ................................ ................................ ................................ .............  15 
C.4.1   Total viable bacterial count  ................................ ................................ ................................ ...............  15 
C.4.2  Examination for the presence of Enterobacteriaceae.  ................................ ................................ .... 15 
C.4.3  Examination for the presence of Staphylococcus aureus . ................................ .............................  15 
C.4.4  Examination for the presence of Pseudomonas aeruginosa  ................................ ..........................  15 
 DUS 1783 : 2017  
iv © UNBS 2017  – All rights reserved  
 Foreword  
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade , Industry and 
Cooperatives established under Cap 327 , of the Laws of Uganda , as amend ed.  UNBS is mandated to co -
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point  on TBT  Agreement  of the World Trade Organisation (WTO).  
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of key stakeh olders 
including government, academia, consumer groups, private sector and other interested parties.  
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews  the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
The committee responsible for this document is Technical Committee UNBS/TC 7, Textile, Leather, Paper and Related 
Prod ucts, Subcommittee SC 1 , Textile and Related products  
 DUS 1783 : 2017  
v  
 Disposable adult absorbent (incontinence) products — 
Specifications   
1 Scope  
This draft Uganda Standard specifies requirements  and test methods  for disposable adult  absorbent products 
for managing inco ntinence including adult diapers, adult briefs , adult under pads (inserted in pants) and others.  
2 Normative references  
The following referenced documents referred to in the text in such a way that some or all of their content 
constitutes requirements of th is document. For dated references, only the edition cited applies. For undated 
references, the latest edition of the referenced document (including any amendments) applies.  
US ISO 139,  Textiles — Standard atmospheres for conditioning and testing  
US ISO 307 1, Textiles — Determination of pH of aqueous extract   
US ISO 4833 -2, Microbiology of the food chain – Horizontal method for the enumeration of microorganisms – 
Part 2: Colony count at 30 C by the surface plating technique   
US ISO 6888 -2, Microbiology of fo od and animal feeding stuffs ─ Horizontal method for the enumeration of 
coagulase -positive staphylococci (Staphylococcus aureus and other species) ─ Part 2: Technique using rabbit 
plasma fibrinogen agar medium  
US ISO 7218 , Microbiology of food and animal feeding stuffs — General requirements and guidance for 
microbiological examinations (2nd Edition)   
US ISO 11948 -1, Urine -absorbing aids — Part 1: Whole -product testing  
3 Terms and definitions  
For the purposes of this standard, the following terms and defin itions shall apply  
3.1  
Incontinence products  
hygienic products specifically developed to help manage bladder or bowel control problems with the capability 
to absorb urine and prevent stool and fluid from leaking out  
3.2  
absorbent filler material  
the mat erial at the core of the diaper that absorbs fluids  
3.3  
Top sheet run -off 
The unabsorbed volume of test solution while running across the surface of inclined specimen   
vi © UNBS 2017  – All rights reserved  
 3.4  
Rewet  
The mass of test solution which returns to surface under specific pressure a fter a certain amount of that being 
absorbed by the specimen  
3.5  
Leakage  
The mass of test solution which penetrates the leak -proof carrier film under specific pressure after a certain 
amount of that being absorbed by specimen  
3.6  Rate of Absor bency  (ROA ) 
Time required to fully absorb test fluid into the test product.  A specified quantity of simulated urine is 
discharged at a prescribed rate under specified  conditions onto a test specimen of an absorbent article with a 
nonwoven cover. The time  taken for t he entire liquid dose to pen etrate the nonwoven is measured  
3.7  Retention Capacity  
A measure of a product’s capacity to hold fluid without  leaking and rewetting the skin  
ISO and IEC maintain terminological databases for use in standardization at the follo wing addresses:  
— ISO Online browsing platform: available at http://www.iso.org/obp  
4 Requirements  
4.1 Materials  
All materials used for making  absorbent products  shall not harm the skin in contact. None of the components 
in the products, including additiv es, should be listed in any Regulatory Agency as being “unsafe’’  
4.1.1  Absorbent filler  
a) it shall have fine ventilative property that keep s the skin dry and comfortable;  
b) it shall be clean, free from harmful foreign materials, lumps, splits, holes and protru ding points when 
visually examined; and  
c) it shall be arranged in a manner that will draw in and trap all moisture rapidly and efficiently and keeps  
it away from the adult’s skin.  
4.1.2  Top Sheet (the layer which contacts adult’s skin)  
a) shall be of material t hat helps absorption, an d shall have no harmful effect ; and  
b) shall cover the absorbent filler completely and prevent the filler from reaching the adult’s skin or 
clothes under normal handling . 
4.1.3  Back sheet  
There shall be an outer cover to prevent direct  contact of the absorbent filler with the adult’s clothing and to 
prevent li quid leakage out of the absorbent product.  
4.1.4 Fastening device (closure system ) 
There shall be a suitable device for fastening the absorbent products for secure use. The closure  system 
regardless of how its functionality is achieved should allow for multiple fastening and unfastening. This 
promote s better fit and allow s for easy check of wetness without having to dispose off and replace unsoiled 
product   
© UNBS 2017  – All rights reserved  vii 
 4.1.5 Breathability  
The pr oduct should be breathable and comfortable to wear. There should be a  minimum air flow sufficient to 
release trapped body heat  or gaseous body per spiration in the pelvic region  
4.2 Key performance requirements  
Adult absorbent products  shall comply with  the performance requirements  given in Table 1  when tested in 
accordance with the methods specified therein.  
Table  1 — Characteristics of disposable  adult  absorbent products  
Characteristics  All size categories  Test methods  
Rate of absorption , s, (max)  85 Anne x A 
Rewet under load , g, (max)  5 Annex A  
Retention capacity (min)  250 Annex  
Absorptive capacity (min)  1200  US ISO 11948 -1 
pH  6 – 8.5 US ISO 3071  
 
4.3 Microbiological requirements  
a) the total viable bacterial count, when determined in accordance with A nnex C  shall not exceed 1 000 
per gram of the absorbent product ; and  
b) when tested, the absorbent product shall be free from Enterobacteriaceae, Staphylococcus aureus, 
and Pseudomonas aeruginosa respectively  
5 Packaging and marking  
5.1 Packaging  
Disposable ad ult absorbent products  shall be packed in a suitable waterproof package that shall protect them 
from any form of contamination and damage.  Packaging for shipment shall be in accordance with the 
manufacturer's standard practice and in a manner readily accep ted by  the market.  Within the shipping carton, 
units shall be packed in manner designed to minimize damage during shipment due to rough or improper 
handling.  
5.2 Marking  
On both the primary and secondary packages, the disposable adult absorbent product  packs shall be marked 
with legible and indelible pre -printed marking or a securely affixed and durable label bearing the following 
information:  
a) name of products;  
b) name and address of the manufacturer  and/or importer/distributor (if applicable) ; 
c) number of pieces in the pack;  
d) size category and dimensions;  
e) instruction for use, storage and disposal ;  
viii © UNBS 2017  – All rights reserved  
 f) date of manufacture and expiry;  
g) batch/ lot number; and 
h) country of origin.  
 
6 Sampling  
6.1 Lot 
In any consignment , all packages of the adult absorbent product  belonging  to one batch of manufacture or 
supply shall constitute a lot.  
6.2 Scale of sampling  
6.2.1 Samples shall be tested from each lot ascertaining its conformity to the requirements of this 
specification.  
6.2.2 The number of packages to be selected from a lot s hall be in accordance with Table 2 . 
Table 2  –scale of sampling  
Number of packages in a lot  Number of packages to be selected  
Up to 250  6 
251-500 8 
501-1000  11 
1001 -2500  15 
2501 -5000  20 
5001 and above  30 
 
6.2.3 The bulk packages and packag es shall be  selected at random  
6.3 Number of tests  
6.3.1 Each package selected as per table 2  shall be inspected for packaging and marking requirements  
6.3.2  Sanitary towels selected as per table 2  shall be examined for requirements stipulated in clause 4 and 
5 
7 Proof of compliance  
The manufacturer or the supplier shall bear the burden of proof of compliance with this specification  
8 Acceptance criteria  
Product acceptance shall be based on the fin ding that all the required test  results meet or exceed the 
specified ta rget values.   
© UNBS 2017  – All rights reserved  ix 
 Annex  A 
(normative)  
 
Rate of absorption and rewet test  
A.1 Scope and Purpose:  
a) To measure the ability of an absorbent products  to accept and retain synthetic urine (saline solution) 
under simulated in -use conditions of load and pressure.  
b) To det ermine amount of time required for an absorbent article to absorb a fixed quantity of a test 
solution  
A.2 Principle  
The method measures the ability of the absorbent products  to accept and retain 0.9% saline solution under 
simulated in -use conditions of loa d and pressure   
A.3 Apparatus and materials  
a) a rigid cover plate with a weight as shown below. Dimensions of the plate 200 mm x 70 mm. Inner 
diameter of cylinder 40 mm, total Weight: 6300 g (plate 605.3 g, weight 5694,7 g) representing a 
pressure of 4.41 kP a (0,64 psi) for all sizes ; 
b) tray; 
c) filter paper; Medium -speed qualitative chemical analysis filter paper; (AFI Grade 950 or Schleicher & 
Schuell Grade 597,  Whatman Grade 2, Camlab grade 113) having a diameter of or approximately 40 
mm and conditioned togeth er with the test samples as above;  
d) graduated cylinder (1 ml graduation) ; 
e) stopwatch ; 
f) ruler (at least 2 cm longer as absorbent core of the sample, 1mm graduation) ; and 
g) a pen. 
A.4 Saline solution  
Add 9 g sodium chl oride, and 1 g of blue dye  into 200 mL distil led water at ambient temperature after whi ch 
the solution is made up to 1 Liter. The pH shall be between 6.2 – 6.7. When not within this range , adjust by 
using 0.1 mol/L sodium hydroxide solution or acetic acid as relevant. The temperature of the solution shall be 
37 ºC ± 2 ºC, and shall be maintained in a water  bath during testing.  
A.5 Saline dosage  
Measure out the volume of saline solution  for each of the products being tested  as follows :  
x © UNBS 2017  – All rights reserved  
 a) adult dis posable diaper (brief) of size: Medium to X X-Large, Single  dose of 200 ml  
b) adult di sposable diaper (brief) of size: Small, Single dose of 100 ml  
c) all sizes of adult disposable  under -pads/ underpants: Single dose of 100 ml  
A.6 Sample preparation and Set -up 
a) conditioning: Bring samples to moisture equilibrium in the s tandard atmosphere for testing nonwovens 
in accordance with US ISO 139 . Equilibrium is considered to have been reached when the increase in 
mass of the specimen in successive weighings made at intervals of not less than 2 hours does not 
exceed 0.2 5 % of th e mass of the specimen;  
b) take 5 test specimens  randomly from the test samples ; 
c) weigh a stack of dry filter paper and record as weight W1. The stack should have a  dry weight of 
about 10.0 grams;  
d) mark the loading point in the middle of the absorbent core. Not e that the absorbent core does not 
cover the full length of the diaper but it is more concentrated in the front of the diaper. Therefore, the 
middle of the absorbent core will not be the same as the middle of the diaper: Measure the length and 
width of the  absorbent core. Mark the midpoint, which will be the loading point;  
e) place core with topsheet facing upward on the tray ; 
f) place rigid cover plate, ensure the plate is centered towards the width of the diaper core and the 
cylinder opening is placed over mark ed loading point. Diaper should be stretched showing minimum 
amount of wrinkles ; 
g) gently place weights on the plate (ideally use weight in a ring form to all ow for equally applied 
pressure;  
h) fill the measuring cylinder with respective amount of saline soluti on; 
i) gently pour the saline onto the diaper starting the stop watch at the same time with the pouring ; 
j) stop the stopwatch as soon as the saline solution has been absorbed by the diaper (all the wet sheen 
has disappeared) and record the result in se conds as the rate of absorption;  
k) leave the diape r undisturbed for 10  min; 
l) lift the weight and cover plate and p lace the filter paper stack on the  absorption point of the diaper;  
m) replace the cover plate and the weight for 2 min on top of the filter pap er stack;  
n) after 2 min, remove the weight and cover plate and immediately determine and record the weight of 
the wet filter paper stack as W2; and  
o) repeat the procedure on the remaining test specimens  recording each result separately;   
© UNBS 2017  – All rights reserved  xi 
 
 
Fig A.3 : apparatus and set up  
A.7 Calculation of results  
A.7.1 Calculate as the rate of absorption in s, the mean time for absorption  recorded in (j) above . 
A.7.2 REWET (g) = W2 (Wet filter paper weight) - W1 (Dry filter paper weight). Report the average of the 5 
products tested  
  
xii © UNBS 2017  – All rights reserved  
 Annex  B 
(normative ) 
 
Test Method for Retention Capacity  
The test method for Retention Capacity requires the following steps:  
A.1 EQUIPMENT  
a) fischer & Paykel Ecosmart Model WA37T26G washing machine or equivalent piec e of equipment. 
Must be capable of a 7 minutes &1 5 second spin cycle at 670 rpm  
b) weighing Tray – large enough for product being tested  
c) lab Balance - capable of weighing to nearest g ram 
A.2 PROCEDURE  
A.2.1  Upon completion of a liquid absorption capacity test , place the wet product in the washer with the 
absorbent c ore facing the side of the tub.  
Note:  Multiple products may be spun at the same time provided  there is no overlapping of the product’s core. If testing 
multiple samples, identify them with in delible  ink for identification.  
A.2.2  Push the power button on the washing machi ne panel to turn the washer on.  
A.2.3  Using the arrow button, select the Spin cycle under the Wash Progress display.  
A.2.4  Select Medium at the spin s peed display, and then push the S tart/ Pause button. Medium spin speed 
is 670rpm and the complete cycl e time is 7 -minutes 15 -seconds.  
A.2.5  The machine’s lid will automatically lock to prevent opening during the cycle and the spin cycle will 
start.  
A.2.6  When the spin cycle is complete, t he machine will beep and the lid will automatically unlock.  
A.2.7  Remove the product, place in a tare weighing tray and record the s pun weight.  
A.3 CALCULATIONS  
A.3.1  Report the amount liquid retained in grams as Retention Capacity  
A.3.2  Retention Capacity  = Spun Weight - Dry Weight (Recorded prior to liquid absorbent capacity test  
 
 
 
 
 
 
  
© UNBS 2017  – All rights reserved  xiii 
 Annex  C 
(normative)  
 
Microbiological examination  
C.1 Apparatus and equipment  
Use apparatus and equipment complying with  the relevant requirements of US ISO 7218.  
C.2 Media and reagents  
C.2.1 General  
Ensure compliance with the general requirements for the ingredients and for the preparation of media and 
reagents given in US ISO 7218   
C.2.2 Bacteriological peptone  
Peptone               10 g  
Disodium phosphate dodecahydrate         1 g  
Sodium chloride             5 g  
Mono -potassium phosphate          1.5 g  
Dissolve the ingredients in distilled water and make up to 1 L. Adjust the pH value to be 7.0 ± 0.1 after 
sterilization . Dispense 300 mL volumes into flasks of capacity 500 mL and sterilize by autoclaving at 121 °C ± 
2 °C for 20 min.  
C.2.3 Plate count agar  
Agar               15 g  
Glucose               1 g  
Tryptone               5 g  
Yeast extract              2.5 g  
Dissolv e the ingredients in distilled water, made up to 1litre, and adjust the pH value to 7.2 ± 0.2. Dispense 15 
mL volumes into bottles and sterilize by autoclaving at 121 °C ± 2 °C for 20 min.  
C.2.4 Neutral red -bile salt peptone glucose medium  
Peptone               20 g  
Glucose               10 g  
Bile salts No. 3             1.5 g   
xiv © UNBS 2017  – All rights reserved  
 Sodium chloride           5 g  
Neutral red            0.03 g  
Crystal violet            0.002 g  
Dissolve the ingredients in 400 mL of distilled water and make up to 500 mL boiling to a id solution. Adjust the 
pH value to 7.4 and filter to a clear solution. Dispense 10 mL volumes into bottles each containing a Durham 
tube and sterilize by autoclaving at 121 °C ± 2 °C for 20 min  
C.2.5 Fluid soybean -casein digest medium  
Pancreatic digest of  casein         17 g 
Papaic digest of soybean meal        3 g  
Sodium chloride           5 g  
Dibasic potassium phosphate        2.5 g  
Dextrose             2.5 g  
Dissolve the ingredients in distilled water and make up to 1 litre, warming slightly to aid soluti on. Cool the 
solution to room temperature and adjust the pH value to be 7.3 ± 0.2 after sterilization. Filter to clarify (if 
necessary), dispense into suitable containers, and sterilize by autoclaving at 121 ± 2 °C for 20 min.  
C.2.6 Centrimide agar medium   
Pancreatic digest of gelatine        20 g  
Magnesium chloride          1.4 g  
Potassium sulphate          10 g  
Agar             13.6 g  
Cetyltrimethylammonium bromide (Cetrimide)    0.3 g  
Glycerine            10 mL  
Dissolve all the solid ingredients in dis tilled water, make up to 1 L, and then add the glycerine. Heat, agitating 
frequently, and boil for 1 min. Adjust the pH value to be 7.2 ± 0.2 after sterilization. Dispense into suitable 
containers and sterilize by autoclaving at 121 °C ± 2 °C for 20 min.  
C.2.7 Pseudomonas agar  medium for detection of fluorescein  
Pancreatic digest of casein         10 g  
Peptic digest of animal tissue        10 g  
Anhydrous dibasic potassium phosphate      1.5 g  
Magnesium sulphate (MgSO4.7H2O)       1.5 g  
Glycerine            10 mL  
Agar             15 g   
© UNBS 2017  – All rights reserved  xv 
 Dissolve all the solid ingredients in distilled water, make up to 1 L, and then add the glycerine. Heat, agitating 
frequently, and boil for 1 min. Adjust the pH value to be 7.2 ± 0.2 after sterilization. Dispense into suitable 
containers and sterilize by autoclaving at 121 °C ± 2 °C for 20 min.  
C.2.8 Pseudomonas agar  medium for detection of pyocyanin  
Pancreatic digest of casein           20 g  
Anhydrous magnesium chloride          1.4 g  
Anhydrous potassium sulphate          10 g  
Agar               15 g  
Glycerine              10 Ml  
C.3   Preparation of  Test Suspension  
Transfer 300 ml of the sterile soluti on of bacteriological peptone (C .2.2) to a sterile wide -mouthed jar of 
capacity not less than 1 litre and not more than 2  litres. The jar shall have a mouth of diameter not less than 
150 mm and not more than 250 mm, and is fitted with a hermetically closing glass or metal -and-glass lid.  
Aseptically place the towel under test in the solution in the jar, fit the lid, agitate the conte nts of the jar for 2 
min and then allo w the jar to stand for 10 min. Repeat this agitating and standing procedure twice more. 
Aseptically remove about 100 ml of the test suspensi on for testing as described in C.4 below.  
C.4  Procedure  
C.4.1   Total viable  bacterial count  
Into each of three sterile petri dishes aseptically pipette a 1 mL portion of the test suspension. To each dish 
add 15 mL of freshly  melted plate count agar  that has been cooled to 45 °C, and mix well. Incubate, count and 
calculate the to tal count as described in US ISO 4833  Part 2. From the total viable bacterial count and the 
mass of the sanitary towel, calculate the total viable bacterial count per gram of sanitary towel.  
C.4.2  Examination for the presence of Enterobacteriaceae.  
Asept ically add 10 mL of the test suspension to a bottle that contains neutral red -bile salt peptone glucose 
medium (C .2.4). Incubate the bottle for 24 h to 36 h at 37 ± 0.5°C and examine for the presence of 
Enterobacteriaceae as evidenced by the formation of ac id and gas.  
C.4.3  Examination for the presence of Staphylococcus aureus .  
Use the media, reagents and procedure described in US ISO 6888 -2 to ex amine the test suspension (see 
C.3). As a control, pipette 0.1 mL of a 1:1000 dilution of an 18 h to 24 h cult ure of Staphylococcus aureus  
SATCC Sta 10 into Staphylococcus  medium and proceed as with the test suspension.  
C.4.4  Examination for the presence of Pseudomonas aeruginosa  
a) Aseptically pipette 10 mL of the test suspension into 90 mL of fluid  soybean -casei n diges ts medium 
(C.2.5) and mix well. Incubate for 24 h at 30 °C to 35 °C. By means of an inoculating loop transfer a 
portion from the 24 h incubated sample tube of fluid soybean -casein digest medium to the dry surface 
of petri dishes each containing appr oximately 2 0 mL of Cetrimide agar medium (C .2.6). Incubate at 30 
°C to 35 °C and examine after 24h, and again after 48 h incubation, for suspect colonies, bearing in 
mind that in general greenish fluorescent colonies are typical of Pseudomonas aureginosa  and that in  
xvi © UNBS 2017  – All rights reserved  
 its presence a gram stain examined microscopically will reveal gram -negative slender rod -shaped 
cells.  
b) As a control, add 0.1 ml of a 1:1 000 dilution of an 18 h to 24 h culture of Pseudomonas aeruginosa  
SATCC Pse 11 mL to 100 mL of fluid  soybea n-casei n digest medium (C .2.5), and proceed as with the 
test suspension.  
c) If none of the colonies obtained from the test suspension conforms to the description given in i) above 
and the control culture has been satisfactorily recovered, deem the test sampl e to be free from 
Pseudomonas aeruginosa.  
d) If colonies conforming to the description given in i) above are found, streak representative suspect 
colonies from the Cetrimide agar onto the surfaces of Pseudomonas agar  medium for  the detection of 
flourescein ( C.2.7) and Pseudomonas agar medium f or the detection of pyocyanin (C .2.8) to obtain 
isolated colonies.  Cover and invert the petri dishes and incubate at 30 – 35 °C for at least 3 days.  
Examine the streaked surfaces under ultraviolet light for suspect c olonies, as described in Table C .1.  
Table C .1 — Description of colonies  
Medium  Description of colonies  
Pseudomonas agar for the detection of fluorescein  Generally colourless to yellowish  
Yellowish fluorescence in ultra violet light  
Pseudomonas agar for the detection of pyocyanin  Generally greenish. Blue fluorescence in ultraviolet light  
 
If any further doubt exists as to the identity of the colonies, obtain final confirmation by inoculating the suspect 
colonies to the wells on commercially available diag nostic kits in accordance with the manufacturer’s 
instructions.   
© UNBS 2017  – All rights reserved  xvii 
 Certification marking  
Products that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the figure below.  
The use of the UNBS  Certification Mark is governed by the Standards Act, and the Regulations made 
thereunder. This mark can be used only by those licensed under the certification mark scheme operated by 
the Uganda National Bureau of Standards and in conjunction with the rele vant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing in accordance with the 
certification ma rk scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually checked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bur eau of Standards.  
 
 DUS 1783 : 2017    
 
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© UNBS 2017 – All rights reserved