DRAFT UGANDA STANDARD  
  DUS 1832  
First Edition  
2017 -mm-dd 
 
 
 Reference number  
DUS 1832 : 2017 
© UNBS 2017   
  
Glycerine  for Cosmetic  use— Specification  
 DUS 1832 : 2017  
ii © UNBS 2017  – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correc t application  
© UNBS 2017   
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 DUS 1832 :2017  
© UNBS 2017  – All rights reserved  iii 
 Foreword  
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade , Industry and 
Cooperatives established under Cap 327 , of the Laws of Uganda , as amended .  UNBS is mandate d to co -
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT  Agreement  of the World Trade Organisation (WTO).  
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of key stakeholders 
including gov ernment, academia, consumer groups, private sector and other interested parties.  
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before  recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
The committee responsible for this document is Technical Committee UNBS/ TC 5, Chemicals and 
Environment products . 
 DRAFT UGANDA STANDARD  DUS 1832 : 2017  
 
© UNBS 2017  – All rights reserved  1 
 Glycerine  for Cosmetic  use— Specification  
1 Scope   
This draft Uganda standard specifies requirements, sampling and test methods for glycerine for cosmetic use. 
2 Normative references   
The following referenced documents referred to in the text in such a way that some or all of t heir content 
constitutes requirements of this document. For dated references, only the edition cited applies. For undated 
references, the latest edition of the referenced document (including any amendments) applies.  
FDEAS 846,  Glossary of terms relating to  the cosmetic industry  
FDEAS 847 - 2, Oils for cosmetic industry — Methods of test — Part 2: Determination of Moisture Content  
FDEAS 847 -7, Oils for cosmetic industry — Methods of test — Part 7: Determination of specific gravity  
FDEAS 847 -16, Oils for cosme tic industry — Methods of test — Part 16 : Determination of Heavy metal 
Content  
FDUS ISO 24153 —.Random sampling and randomisation procedures  
US EAS 346 , Labelling of cosmetics — General requirements  
US EAS 377 -1, Cosmetics and cosmetic products — Part 1: L ist of substances prohibited in cosmetic 
products  
US EAS 377 -2, Cosmetics and cosmetic products — Part 2: List of substanc es which cosmetic products must 
not contain except subject to the restrictions laid down   
US EAS 377 -3, Cosmetics and cosmetic produc ts — Part 3: List of colorants allowed in cosmetic products   
US EAS 377 -4, Cosmetics and cosmetic products — Part 4: List of preservatives allowed in cosmetic products   
US EAS 377 -5, Cosmetics and cosmetic products — Part 5: Use of UV filters in cosmetic p roducts  
3 Terms and definitions   
For the purposes of this document, the terms and definitions given in FDEAS 846 , the following term and 
definition apply. ISO and IEC maintain terminological databases for use in standardization at the following 
addresses: — ISO Online browsing platform: available at http://www.iso.org/obp  
3.1  
specific gravity  
ratio of the density of a material to the density of a standard material such as water (for liquids) or air (for 
gases)  DUS 1832 : 2017  
2 © UNBS 2017  – All rights reserved  
 4 Requirements  
4.1 General requirement  
The product shall be clear syrupy  liquid  containing essentially of glycerol  and having a sweet warm taste. I t 
shall also be free from  free from sediment, suspension and other  foreign matter. The  product shall comply with 
the requi rements in US EAS 377 -parts 1 -5. 
4.2 Solubility  
The product  shall be miscible in all proportions with water and with ethyl alcohol (90%v/v) and shall not be 
soluble in diethyl ether, chloroform  and fatty oils  
4.3 Specific q uality requirements  
The product  shall comply with the requirements  given in table 1  
Table 1 — Specific q uality requirements  
Characteristics  Requirement  Test method  
Glycerol ,percent by mass, Min  98 Annex A  
Water  content , percent by  mass, max  2 FDEAS 847 - 2: 
Specific gravity, at 2 5 °C  1.2490 -1.2636  FDEAS 847 -7 
Ash, percent by mass, Max 0.01 FDEAS 847 -15  
Alkalinity ( as Na 2O ), percent by mass, Max 0.01 Annex B  
Chlorides ( a s Cl ) , ppm, Max 10 Annex C  
Sulphates ( as SO4 ), ppm, Max 10 Annex D  
Fatty acids and esters ( as Na 2O ), percent by mass, Max 0.06 Annex  E 
 
Heavy metals ppm, Max Lead — 5  
FDEAS 847 - 16 Arsenic — 2 
Mercury — 2 
 
4.4 weights and measures  
The fill and volume of the material shall comply with  the requirements of the  Weights and Measures Act. 
5 Packing and labelling  
5.1 Packing  
The conta iner (including the closure) in which the glycerine  is packaged shall not react  chemically with  the 
glycerine  and shall be strong enough to protect the product  adequately during handling , transportation and 
storage . 
5.2 Labelling  
In addition to the labelli ng requirements outlined in US EAS 346 , the package shall be legibly marked  with the 
following information:  DUS 1832 : 2017  
© UNBS 2017  – All rights reserved  3 
 a) manufacturer's name and physical address;  
b) product name , “glycerine”, 
c) net mass of the product  when packed;  
d) batch number;  
e) country of origin;  
f) instructi on for use;  
g) Date  of manufacture and expiry ; and  
h) Precaution/warning . 
6 Sampling  
Representative  samples of the product shall be drawn randomly for test in accordance with FDUS ISO 24153  
from the market, factory or elsewhere.  
 DUS 1832 : 2017  
4 © UNBS 2017  – All rights reserved  
 Annex  A 
(normative)  
 
Determinat ion of glycerol content  
A.1 Apparatus  
A.1.1  Burette  
A.1.2  Pipette  
A.1.3  500 ml capacity conical flasks with glass stopper  
A.1.4  weighing pipette / bottle  
A.2 Reagents  
A.2.1  Accurately standardized 0.1  N sodium hydroxide solution .  
A.2.2  0.05 N sodium hydro xide  
A.2.3  0.05% m/v Phenol red indicator   
Dissolve 0.05 g of phenol red in 10 ml of ethyl alcohol and make up to100 ml with water  
A.2.4  0.2 N dilute  sulphuric acid . 
A.2.5  Sodium metaperiodate solution  
Dissolve 70 g of sodium metaperiodate in one litre of  water containing 10 ml of 1 N sulphuric acid  and store 
the solution in an amber coloured bottle in the dark.  
NOTE — Sodium metaperiodate shall be a white crystalline powder containing not less than 98 percent sodium 
metaperiodate.  
A.2.6  Ethylene glycol  
Neutral and free from glycerol  
A.2.7  Standard glycerol  
A.3 Procedure  
A.3.1  Using a weighing pipette or bottle, transfer into a conical flask a well mixed and accurately weighed 
sample.  
A.3.2  Add 100ml of carbon dioxide - free water and 3 drops of phenol red i ndicator solution and acidify with 
sulphuric acid solution to define yellow colour. Heat to boiling and cool to room temperature.  DUS 1832 : 2017  
© UNBS 2017  – All rights reserved  5 
 A.3.3  Adjust the pH value to 8.0 ± 0.1 using 0.05 N sodium hydroxide solution dropwise until the colour 
changes to just pink. If the colour of the original solution interferes with the detection of colour change of the 
indicator, use a pH meter.  
A.3.4  Pipette accurately 50ml of sodium metaperiodate solution, replace the glass stopper, mix by swirling 
gently  and allow the flask to  stand in the dark for 3 0 minutes.  
A.3.5  Wash down the sides of the flask with water, add 5 ml of ethylene glycol, replace the stopper, swirl 
gently and allow the flask to stand in the dark for further 20 minutes and titrate with standard ized 0.05 N  
sodium  hydroxide solution.  
A.3.6  Carry out a blank test simultaneously under similar test conditions  
A.4 Calculation  
Glycerol content, Percent by mass = 
MNBS ) (. 2099  
Where;  
S volume in ml of standard sodium hydroxide solution required for the sample,  
B  volume in ml of standard sodium hydroxide solution required for the blank,  
N normality of standard sodium hydroxide solution, and  
M  mass in grams of the sample taken for test  
Each m l of 0.05 N sodium hydroxide is equivalent to 9.210 mg of C3H8O3.  DUS 1832 : 2017  
6 © UNBS 2017  – All rights reserved  
 Annex B 
(normative)  
 
Test for alkalinity  
B.1  Apparatus  
B.1.1 Conical flask — 500ml  
B.1.2 burette  
B.2 Reagents  
B.2.1 Standardized 0.1N Hydrochloric acid  
B.2.2  Phenolphthalein indicator solution  
B.3 Procedure  
B.3.1 Weigh accurately about 100 g of a well mixed sample into 500 ml conical flask and add 150 ml of 
carbon dioxide free water  
B.3.2 Titrate with standardized 0.1N HCl using phenolphthalein indicator.  
B.4  Calculation  
Alkalinity (Na 2O), percent by mass = 
MNA3.1  
Where:  
A Volume in ml of s tandardized 0.1N HCl  
N Normality of standardized 0.1N HCl  
M mass of sample  taken DUS 1832 : 2017  
© UNBS 2017  – All rights reserved  7 
 Annex C 
(normative)  
 
DETERMINATION OF CHLORIDES  (Volumetric Method)  
C.1 Apparatus  
250 ml capacity conical flasks  
C.2 Reagents  
C.2.1 35 % v/v solution of nitric acid  
C.2.2 Nitrobenzene  
C.2.3 Accurately standardized.  0.05 N  silver nitrate solution   
C.2.4 Accurately standardized. 0.05 N ammonium thiocyanate solution  
C.2.5 saturated, aqueous Ferric ammonium sulphate solution  
C.3 Procedure  
C.3.1 Weigh accurately about 20 g of the  sample in  a 250 -ml conical flask and add about 100 ml of water.  
C.3.2 To this solution,  add 5 ml of nitric acid, a few drops of nitrobenzene and 5 ml of standard  0.05 N silver 
nitrate solution.  
C.3.3 Swirl and titrate with standard ized 0.05 N  ammonium  thiocyanate solution using 1 ml of ferric 
ammonium sulphate solution as  indicator. Carry out a blank test using the same reagents.  
C.4  Calculation  
Chlorides (as Cl), parts per million =
MN)SB( 50035
  
Where : 
B  volume in ml o f standard ammonium thiocyanate solution  required for the blank,  
S volume in ml of standard ammonium thiocyanate solution  required for the sample,  
N normality of standard ammonium thiocyanate solution, and  
M  mass in g of the material taken for the test.  DUS 1832 : 2017  
8 © UNBS 2017  – All rights reserved  
 Annex D 
(normative)  
 
Determination of sulphates  
D.1 Apparatus  
D.1.1 25-ml capacity  Nessler Cylinders   
D.1.2 1 ml capacity Pipette with 0.01 ml graduations  
D.2 Reagents  
D.2.1 Hydrochloric Acid  
1: 1 solution of hydrochloric acid in water.  
D.2.2 Barium Chlor ide Solution  
10 percent (m/v), aqueous  
D.2.3 Standard Sulphate Solution  
Dissolve 1.479 g of anhydrous sodium sulphate in water and make up to 1 litre in a volumetric flask. Dilute this 
stock solution 10 times immediately before use. One millilitre of this  solution contains 0.000 1 g of SO 4. 
D.3 Procedure  
D.3.1 Weigh accurately about 10 g of the sample into a Nessler cylinder, add 1 ml of hydrochloric acid and 2 
ml of barium chloride solution, and make up to 25 ml with water. Allow to stand for 30 minutes. A dull white 
turbidity is produced if sulphates are present.  
D.3.2 Simultaneously, introduce 0.5 ml, 0.75 ml, 1.0 ml and 1.25 ml of standard sulphate solution into 4 
separate Nessler cylinders. In each, add 1 ml of hydrochloric acid and 2 ml of barium chlo ride solution, and 
make up to 25 ml with water. Allow  to stand for 30 minutes.  
D.3.3 Note which one of these four solutions has a turbidity most closely matching  that produced by the test 
material (see F.3.1) . Repeat the test and note the volum e of standar d sulphate solution required to produce a 
turbidity exactly matching that of the test sample.  
D.4 Calculation  
Sulphates (as SO 4), parts per million =
MV 100  
Where; 
V = volume in ml of standard sulphate solution used, and  
M = mass in g of th e material taken for the test.  DUS 1832 : 2017  
© UNBS 2017  – All rights reserved  9 
 Annex E 
(normative)  
 
Determination of fatty acids and esters . 
E.1 Apparatus  
E.1.1 500-ml round -bottomed flask  
E.1.2 pH meter  
E.2 Reagents  
E.2.1 Accurately standardized  0.25 N sodium hydroxide solution  
E.2.2 Accurately standar dized 0 25 N sulphuric acid  
E.2.3 phenolphthalein indicator solution  
E.3 Procedure  
E.3.1 Weigh accurately about 50 g of the sample into a  500-ml round -bottomed flask. Add 100 ml of hot, 
carbon dioxide -free water and 1 ml of phenolphthalein indicator solut ion. If  the solution is  alkaline, neutralize it 
with sulphuric acid  
E.3.2 Add exactly 15.0 ml of  standard sodium hydroxide solution, connect the flask to a reflux  condenser 
and heat to boiling. Boil for 5 minutes, allow to cool slightly  and wash down the condenser with a little water. 
Disconnect the flask,  close it with a stopper carrying a sodalime tube, and cool.  
E.3.3 Titrate with  standard ized 0.25 N sulphuric acid and perform a blank determination.  
E.4 Calculation  
Fatly acids and esters, as Na2O, percent by mass =
MNSB ) (3.1  
Where; 
B volume in ml of standard sulphuric acid required for the  blank,  
S volume in ml of standard sulphuric acid required for the  material,  
N normality of standard sulphuric acid, and  
M mass in g of the material taken  for the test  DUS 1832 : 2017  
10 © UNBS 2017  – All rights reserved  
 Bibliography  
[1] British pharmacopeia , 2015  
[2] Glycerine: an overview, 1990 by The Soap and Detergent Association  
[3] IS 1796 (1986): Glycerine - specification  
[4] IS 12590 (1988): Glycerine for Cosmetic Industry  
[5] Physical Properties of G lycerine  and Its Solutions  
[6] USP 29 - NF 24 page 1011, pharmacopeial forum volume 28 (4) page 1245  
 DUS 1832 : 2017  
© UNBS 2017  – All rights reserved  11 
 Certification marking  
Products that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the figure below.  
The use of the UNBS Certification Mark is governed by the Standards Act, and the Regulations made 
thereunder. This mark can be used only by those licensed under the certification mark scheme operated by 
the Uganda National Bureau of Stan dards and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing i n accordance with the 
certification mark scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually checked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
 DUS 1832 : 2017    
 
ICS 71.100.70 
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© UNBS 2017– All rights reserved