2016-781
()
2016. 12. 27.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 1 -
2016-781
  ( 2016-124 ｢ ｣
, 2016.11.16.)
, 46 ｢ ｣
 .
20161227
() ｢ ｣
1. 
  “
” 3 , “ ” 
21 , “ ”
 ·
2. 
 . 
“ ” 3
  1) 
 , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 2 -
  2) 
 “
” (. 4. ., II. 5. Ⅱ
. 
 )
  3) 
 · “
” (. 4. ., II. 5. . Ⅱ
)
  4) “ ” (. 4. Ⅱ
., II. 5. . )
  5) 
 . 
 “ ” 2
  1) 
  2) 
 pH 
(I. 2. (2)(3), 3. 15)) ∼
  3) “
 ( )” “Aspergillus melleus
” 
 (II. 4. . )
  4) “
 ” (II. 4. . 
)
  5) 
 . “ ” 21
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 3 -  1) 
  2) “ ” 6
“ ” (II. 5. . , 
, , , , 
)
  3) 
“ ” 3 , ·
,
, 
 6 (II. 5. . 
,
 , , , , 
,
 , , 
)
  4) 
“5- ” ′
“ ” ( )
(II. 5. . 5- , ) ′
  5) ( )
“ ” 3 (II. 5. . , 
.... , .... )
  6) 
 ·
“ ” (II. 5. )
  7) 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 4 -
 . 
  1) 
  2) (IV. 8. 
)
  3) 
3. 
  ｢
 () ｣
2017227
 ( : 28159, : 
2187 
, :
 , 043-719-250211, ～
043-719-2500) .
  . (· )
  . ( ), 
  . 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 5 -
2017-  
  7 1 ( ｢ ｣ ｢ ｣
2016-124, 2016.11.16.)
 .
                                             2017
                                                 
() ｢ ｣
.
I. 2. 3) (2)“ ”“ ”, “ ”“ ”
, “ ”“ ” .
I. 2. 3) (3) .
 (3) “ ” “ ”
40% .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 6 -
I. 3. 15) .
 15) 
 , , 
 . “ ”, “ ”, “
”, “ ”, “ ”, “ ”, “ ”
pH
 .
     
3
 7.5 9
3 5
 9 11
5 6.5
 11
6.5 7.5
II. 4. . 
.
(6S)-5-Methyltetrahydrofolic Acid, Glucosamine Salt
: C32H51N9O16
: 817.80
: 5-MTHF-glucosamine CAS No.: 1181972-37-1
3446%, ～
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 7 -
(6S)-5-methyltetrahydrofolic acid 5459∼
%
.
  
 ∼
 .
. (1)
, .
(1) : 5.0g
, 2.0ppm 
.
(2) : 
 , 
 2.6ppm 
.
(3) : , 0.1ppm 
.
(4) : 
 5.0g
, 1.0ppm 
.
(5) : 2g
500mL 20mL 
30mL
10mL . 23mL～
. ～
 75mL 
25mL
50mL . , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 8 -
. 
, 「
」 
, 10ppm 
(, 
).
(6) (6S)-5-Methyltetrahydrofolic acid : 
 35mg
90mL 20 1 ℃
100mL . 5mL
10mL 10mL
0.45
m . μ
  25mg
(6R,S)-5-Methyltetrahydrofolic acid
 calcium salt
90mL 20 1 ℃
100mL . 5mL
10mL 10mL
0.45
m μ
 . 
, 
(6S)-5-Methyltetrahydrofolic acid
(%)99% . 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 9 -(6S)-5-Methyltetrahydrofolic acid(%) 
=(6S)-5-Methyltetrahydrofolic acid
×100  (6S)-5-Methyltetrahydrofolic acid
(6R)-5-Methyltetrahydrofolic acid
   
: 
 ( 225nm)
: 
Chiral HSA(Chromtech, 4.0mm×100mm, 5m) μ
: 30°C 
: 100mM NaH2PO4
10% NaOH pH 7.0
.
 940mL2-propanol 60mL
.
: 0.7mL/min
(6) : 
 「 」
( ) , 1g100 
.
(7) : 
 「 」 
, (-) .
(8) : 
 「 」 
( ) , 1g 
100 .
(- ) , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 10 -
8.0% .
(1) : 
 0.5g 100mL 
: (75:25) 50mL
100mL
 . 
0.45
m . μ
 D-
0.5g
 : (75:25) 50mL
100mL
. 0.45
m . μ
, . 
(%) =Ac × Pstd × T% × 100
Astd × Pc × (100 - m)
Ac :  
Pstd :  (mg)
Astd :  
Pc : (mg) 
T% : (%)
m : (%)
   
: UV 195nm
: Shodex Asahipak NH2P-50 4E(4.6mm × 250mm, 5μ
m)
: 35°C 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 11 -
: A : B = 75 : 25
:
 A:
   
B:  
: 1.0mL/min
(2) (6S)-5-Methyltetrahydrofolic acid 
: 70mg
100mL
0.45
m μ
 .
(6S)-5-Methyltetrahydrofolic acid
 calcium(6S)-5-Methyltetrahydrofolic 
acid
 40mg
 100mL 
100mL
. 
, . 
(%) =Ac × Pstd × T% × 100
Astd × Pc ×  (100 - m)
Ac :  
Pstd :  (mg)
Astd :  
Pc : (mg) 
T% : (6S)-5-Methyltetrahydrofolic acid 
(%)
m : (%)
   
: UV 280nm
:Gemini phenomenex(4.6mm×250mm, 5m) μ
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 12 -
: 25°C 
:
 A: KH2PO4 
6.8g 350mL
20% KOH pH 6.5 1000 mL
.
   
B: KH2PO4 
4.08g 650mL
, 350mL . 20% KOH pH 
8.0 .
   
()
 A(%)
 B(%)
0 100 0
15 60 40
17 30 70
22 30 70
31 100 0
: 1.0mL/min
II. 4. . 
.
Branching Glycosyltransferase
1,4--Glucan Branching Enzyme α
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 13 -
Rhodothermus obamensis
Bacillus subtilis
. , , , 
. 
  
 -1,4- α
-1,6- . α
 , , ∼
. ∼
.
 (1) : 
 , 
4.0ppm .
  (2) : 
 5.0g
, 5.0ppm .
  (3) : 「 」
, 1g30 
.
  (4) : 「 」
, (-) .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 14 -  (5) 
 : 25g 「 」
 , (-)
.
()
  : 
 1-6-α 
, Branching Enzyme 
Units(BEU) . 1 BEU (pH 7.2, 
60) 660nm - complex ℃
.
  : 1g(± 0.5mg)
Tris-HCl 80mL 30
100mL Tris-HCl
100mL . Tris-HCl 
, 3050 ∼
BEU/mL . (D)
( 1040 ). ∼
.
  : .( 4
) 50L (Vs=0.050mL)50L μ μ
 , 50L 50L μ μ
, 100L . μ
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 15 -
60 30(t) ℃
, 30 2mL stop reagent
20 660nm
. 4
( :As, :AR
, 
:AB)
    0.150.3 . ※ ∼
0.1M Tris-HCl(pH 7.2) 
.
  .
(BEU/g) =(ARAs)×V×D×100 –
(ARA–B)×t×Vs×W
(AR
-As): 
(AR-AB
): 
V: (mL)
D: 
100: BEU/g
t: ()
Vs: (mL)
W: (g)
  : Branching Enzyme Units(BEU)
(pH 7.2, 60) 660nm - ℃
complex , 1%
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 16 -
II. 4. . 
.
Black Carrot Extract
: Black carrot color INS No.: 163(vi)
(Daucus carota L. ssp. sativus
var. atrorubens
 Alef.) , , 
(Anthocyanin)
. , , , 
.
  (Ecm
) .
, , 
.
  ～
, 500550nm . ～
 (1) 
 : , 
4.0ppm .
  (2) : 
 5.0g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 17 -
, 2.0ppm 
.
  (3) : 5.0g
, 1.0ppm 
.
  (4) : 0.1g , 
1.0ppm .
  (5) : (3) , 「 」
1 (Ecm
) 0.005% .
( )  
 0.30.7～
pH 
3.0 ·
100mL
 . 
. pH 3.0 ·
1cm, 500550nm ～
A .
(Ecm)＝A × 10
(g)
    · (pH 3.0)
      1 (0.1M ) : 1L 21.01g (C6H8O7
H‧2
O) .
      2 (0.2M ) : 1L 71.63g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 18 -
(Na2HPO412H‧ 2
O) .
      1 59 2 41 pH 3.0
.
II. 4. . .
Furcellaria fastigata
 HUD.
( , , 
)
 , 
.
II. 4. . “ ”“ , Aspergillus 
melleus
 ” , . ( )
() .
  , Aspergillus melleus
 2 
.
II. 5. . 
 5“ , 
,
 · ”“ , ” , 6
, 67 “ ”“ ”, “
”“ ” , 78 , 8 11
9 12 , “12. ”“13. ”
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 19 -
, 13 15 14 16 , 1617
.
 6. 
· : 0.030g/kg(, 5
 0.150g/kg)
 8. , : 0.50g/kg(, ( 「 」
) () ( 「 」
) ·
( ) )
 17. 
 (, ), ,
, , , , , , , ․
,
 , , ( , 
 ), , , , , , , 
, : 0.030g/kg
II. 5. . 
 5“ , ,
· ”“ , ” , 6
, 67 “ ”“ ”, “ ”
“ ” , 78 , 8 11
9 12 , “12. ”“13. ” , 13
15 14 16 , 1617
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 20 - 6. 
· : 0.030g/kg(, 5
 0.150g/kg)
 8. , : 0.50g/kg(, ( 「 」
) () ( 「 」
) ·
( ) )
 17. 
 (, ), ,
, , , , , , , ․
,
 , , ( , 
 ), , , , , , , 
, : 0.030g/kg
II. 5. . 
.
II. 5. . 
 5“ , ,
 ·
”“ , ” , 6
, 67 “ ”“ ”, “ ”“ ”
, 78 , 8 11 9 12
.
 1. 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 21 -
 , “12. ”“13. ” , 13 15
14 16 , 1617 .
 6. 
· : 0.030g/kg(, 5
 0.150g/kg)
 8. , : 0.50g/kg(, ( 「 」
) () ( 「 」
) ·
( ) )
 17. 
 (, ), ,
, , , , , , , ․
,
 , , ( , 
 ), , , , , , , 
, : 0.030g/kg
II. 5. . 
.
II. 5. . 
 5“ , ,
· ”“ , ” , 6
II. 2. 1) .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 22 -
, 67 “ ”“ ”, “ ”
“ ” , 78 , 8 11
9 12 , “12. ”“13. ” , 13
15 14 16 , 1617
.
 6. 
· : 0.030g/kg(, 5
 0.150g/kg)
 8. , : 0.50g/kg(, ( 「 」
) () ( 「 」
) ·
( ) )
 17. 
 (, ), ,
, , , , , , , ․
,
 , , ( , 
 ), , , , , , , 
, : 0.030g/kg
II. 5. . 
 4 , 6“ ”
 “ , · ” , 78 , 7
, 8“ ”“ ” , 9 16
10 17 .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 23 - 4. 
 (, 8% ), , , 
,
 , , , (, ), 
, 
 , , ( ), , 
,
 1.0g/kg (, , ∶
1.0g/kg 
, 0.2g/kg 
)
 7. : 0.5g/kg ( , , 
0.6g/kg , 
0.5g/kg )
II. 5. . 4 , 6“
”“ , · ” , 78 , 7
, 8“ ”“ ” , 9
16 10 17 . 
 4. 
 (, 8% ), , , 
,
 , , , (, ), 
, 
 , , ( ), , 
,
 1.0g/kg (, , ∶
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 24 -
1.0g/kg 
, 0.2g/kg 
)
 7. : 0.5g/kg ( , , 
0.6g/kg , 
0.5g/kg )
II. 5. . 4 , 6“
”“ , · ” , 78 , 7
, 8“ ”“ ” , 9
16 10 17 . 
 4. 
 (, 8% ), , , 
,
 , , , (, ), 
, 
 , , ( ), , 
,
 1.0g/kg (, , ∶
1.0g/kg 
, 0.2g/kg 
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 25 -
 7. : 0.5g/kg ( , , 
0.6g/kg , 
0.5g/kg )
II. 5. . 
 5“ , , ·
”“ , ” , 6
, 67 “ ”“ ”, “ ”“
”
 , 78 , 8 11 9
12 , “12. ”“13. ” , 13
15 14 16 , 1617
.
 6. 
· : 0.030g/kg(, 5
 0.150g/kg)
 8. , : 0.50g/kg(, ( 「 」
) () ( 「 」
) ·
( ) )
 17. 
 (, ), ,
, , , , , , , ․
,
 , , ( , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 26 -
 ), , , , , , , 
, : 0.030g/kg
II. 5. . 1“ ”“ , 
· ” , 2“ ( )”“ ”
.
 (
 , 
0.6g/kg , 
0.5g/kg )
II. 5. . 1“ ”“
, · ” , 2“ ( )”“
” .
 (
 , 
0.6g/kg , 
0.5g/kg )
II. 5. . 1“ ”“
, · ” , 2“ ( )”“ ”
.
 (
 , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 27 -
0.6g/kg , 
0.5g/kg )
II. 5. . 1“ ”“
, · ” , 2“ ( )”“ ”
.
 (
 , 
0.6g/kg , 
0.5g/kg )
II. 5. . 5- 2 ′
.
 2. : 8g/kg 
II. 5. . 3“ ”“
” , 4“ ”“ ” .
II. 5. . .... 6“ ”
“ ” .
II. 5. . .... 6“ ”
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 28 -
“ ” .
II. 5. . 
 5“ , ,
· ”“ , ” , 6
, 67 “ ”“ ”, “ ”
“ ” , 78 , 8 11
9 12 , “12. ”“13. ” , 13
15 14 16 , 1617
.
 6. 
· : 0.030g/kg(, 5
 0.150g/kg)
 8. , : 0.50g/kg(, ( 「 」
) () ( 「 」
) ·
( ) )
 17. 
 (, ), ,
, , , , , , , ․
,
 , , ( , 
 ), , , , , , , 
, : 0.030g/kg
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 29 -
II. 5. . 
 7
, 8“ ”“ ” .
( , 
1.0g/kg 
, 0.2g/kg 
)
II. 5. . 
 7
, 8“ ”“ ” .
( , 
1.0g/kg 
, 0.2g/kg 
)
II. 5. . 4
.
(, 0.3% )
II. 5. . 
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 30 -
II. 5. . (2) ( ) “ (Potassium 
Hydroxide)” .
IV. 8. . 1) 5) 2) 6) , 1)
.
 1) 
  . , 
.
  
(1) 1
   
5mL , .
.
 1. 
5‘- , , , , 
, L- , , , , 
L- , L- , L- , 5'-
, D- , , D- , , 
, , DL- , DL- , 
, L- , , , D- , D- , 
, 5'- , L- , L- , 
, , DL- , L- , , 
, , , , , 
5'- , 5’- , , 
, , , , 
, , , , 0.25g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 31 -  
  
(2) 2
   
 , , 
, DL- , L- , , 
, , , , , L-
, , , , 
, , L- , , 
, , , , , , 
, L- , L-
0.33g
L- 0.4g
, 0.5g
, , , 0.77g
D- 1g
, 0.25g
(200, 4hr ℃
)
-- , , L- , , , δ
.... , .... , DL-
, L- , , DL- , 
, , 
0.25g, 10mL
0.25g, 25mL
0.38g, 20mL
0.39g( ),
20mL
0.77g, 10mL
0.25g, 10mL
5mL, 50mL
5mL 
, 100mL
25mL 
0.5g, 5mL
10mL 5mL 
0.66g, 10mL
5mL 
10g, 200mL
5mL 
, 100mL
 12.5mL 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 32 -
(150) →
10mL
450550 . ∼ ℃
450550 . ∼ ℃
 3mL
.
, , , , , , 
, , , - , , , - β α
, , , 
, , , L- , 
, , , , 
, , , , 5'-
, , , , , 
, , , , , , 
, , , - , β
, , dl- , l- , , 
, , , , , , 
, , , , 
, B2
 , C, 
E, , , , , 
, , , , 
, , , L- , 
, , , , , 
, , - , - α α
, L- , L- , L-
, , L- , L- , 
, - -8'- , , , β
, , , , 
, , , , 
, , , , 
, , , , 
- , , , , , γ
, , , , , 
, , , , 
, , , , 
, , , DL- , L- , 
, , , , , 
, , , , , 
, , - , , , β
, , , 
, , , , , , 0.25g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 33 -  (3)
.
()
0.25g 5mL
15mL
.
0.25mL 10mL
.
0.5g 5mL 1mL 10mL
2mL
10mL 5mL .
0.5g 5mL, 1mL 10mL
2mL 10mL 
5mL .
0.77g 5mL
.
, , , , , , 
, , , , d-
( ), , , 
, , L- , , 
, , , , 
, , , , , , 
, , , , 
, - , , 20, ε
60, 65, 80, 
, , , , 
, , , , 
, , , , , 
, , , , , 
, 0.38g
, , , , 
, , , , , , 
, , , , , dl-α-
, , , , 
, 0.5g
, , , , , , 
0.77g
, 1g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 34 -
2.5g250mL 0.5N 50mL
. 
30
No. 3 . 
10mL 3
100mL 10mL
.
5g 50mL 50 15 ℃
. 
100mL
2mL .
0.25g 20mL .
1.25g 10mL 
10mL . 
2mL . 
. ～
15mL
. 25mL
5mL .
0.2g , 
5mL . 
5mL
.
0.5g 0.3g
1mL ․
400 . ℃
2mL 10mL ․
5mL .
5‘-
5'-
5'-
0.25g 5mL .
0.25g 150mL 10mL
10mL 5mL
5mL
10mL
. 
10mL
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 35 -
2.5g 25mL
5mL 1mL 2mL
10mL 5mL
.
5g 100mL
5mL (14)→
5mL
.
DL-
L-
0.5g500mL 5mL 
5mL 23mL～
. ～
15mL
23mL . 10mL ～
5mL .
0.25g 5mL 30
5mL , 
.
0.5g 5mL 15
70 . ℃
5mL 10mL
20mL 10mL
.
0.77g 10mL 15
70 . ℃
10mL, 20mL
40mL 20mL
.
A
1g 20mL
. 5mL
. 
5mL . 
. ～
15mL
. 20mL 5mL
.
B2
 .
1.25g 10mL 
5mL . ～
. 
10mL 15mL
. 25mL
5mL .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 36 -
2.5g250mL 
(125) 100mL →
15
. 
200mL 
(125) 25mL 4 →
(125) 200mL →
20mL .
0.25g (12) 10mL →
25mL .
0.25g (14) 5mL →
.
0.25g 15mL
.
1g (12) 30mL 1mL →
5mL
15mL 3
50mL . 25mL
1mL
10mL 2mL
5mL .
0.25g 5mL 2mL
.
50g 250mL
. 1.3mL 5mL
.
20% . 
1.3mL 5mL
.
0.25g 5mL
.
0.25g (12) 5mL →
.
0.25g .
L-
1g100mL 5mL 
5mL . 23mL～
. ～
15mL
23mL . 10mL ～
5mL .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 37 -
1g 5mL 1mL
10mL
2mL
5mL .
10g250mL 0.5N 50mL
. 
15
No. 4 . 
10mL 4
100mL 2.5mL
.
0.25g 5mL 2mL
5mL
.
2mL 250mL (A)
. 1mLCl2
 1g . (A) 10mL
100mL 2.5mL
.
0.5g 20mL L- 0.2g
.
5g 30mL (120) →
6mL
50mL 5mL
.
10.0g
0.5N 50mL 15
. 100150mL ～
1015～
(Whatman No. 4 )
100mL . 1015mL ～
. 2
100mL 2.5mL .
10g250mL (120) 50mL →
15
10mL3
1
015mL , ～
100mL 7.5mL .
L-
0.25g1N 10mL
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 38 -
0.25g500mL 5mL 
5mL . 23mL～
. ～
15mL
23mL～
.
L-
0.25g500mL 5mL 
5mL . 23mL∼
. ∼
15mL
23mL∼
.
0.5g 25mL 1mL 
10mL 2mL
10mL 5mL
.
0.5g
10mL , 1mL 10mL 2mL
10mL
5mL .
0.5g 10mL , 1mL 10mL
2mL
10mL 5mL .
0.25g 10mL
.
10g (14) 60mL →
15mL
1.5g 30mL
1 . 
1 3 10mL 5
(14) 50mL 5mL →
.
2.5g 10mL 5mL
2mL 50mL . 5mL
1mL
5mL .
2.5g 25mL 5mL
1mL 2mL
10mL 5mL .
0.25g 5mL 
5mL . 23mL～
. ～
, 15mL
23mL , ～
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 39 -
0.25g 5mL . 1mL
.
()
1g 10mL 2mL
20mL 5mL
.
0.25g (14) 5mL →
. (14) 5mL 10mL →
.
DL-
L-
0.25g1N 3mL 2mL
.
5g 50mL 50 15 ℃
. 
100mL
2mL .
L-
0.25g2N 10mL
.
0.25g(, 0.25g )
5mL 5mL
. 23mL～ ～
. 
15mL
23mL～
.
1g 10mL, 10mL
30mL 100mL 
100mL 25mL
.
12.5mL 5mL, 
1mL 10mL 2mL
, 5mL
.
0.25g 5mL 2mL 1.5g
5 L- 0.2g
.
1g 6mL 30mL
40mL
10mL .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 40 -
2g500mL 20mL 
30mL
10mL 23mL～
. ～
75mL 25mL
. 50mL
, 
100mL 
100mL . 20mL . 
, , 5mL
100mL
 20mL
. , 6mL
. , 
3mL .
0.5g 5mL 
5mL . 23mL～
. ～
, 15mL
23mL , ～
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 41 -
<2017-  , 2017. . >
1( ) 
 . ① 
  ② 
1 II. 5. . , 
,
 , , , 
17( 16) IV. 8. 
201811 .
2( ) 
 ··
( ) , 
( “ ” ) .
3( ) 
.
4(
 ) 
···( )
(
) , 
·
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 42 -신구조문 대비표 ․
()
I. 
 1.
 ( )
 2. 
  1) 2) ( ) ∼ 
  3) ( )
   (1) ( )
   (2) “
 ” , 
, , , 
40% 
, (chips) 
.
  (3) 
“ ” , , 
40% 
.
   (4) (18) ( ) ∼ 
3. 
 1) 14) ( ) ∼ 
I. 
 1.
 ( )
 2. 
  1) 2) ( ) ∼ 
  3) ( )
   (1) ( )
   (2) “
”------------ 
--------- 
----------- 
-----------------------
----- 
------------------- 
-----------------------
-----------------------
-----------------------
-----------------------
-------
  (3) 
“ ” “
”
--------------------
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 43 -
()
 15) , 
, 
.
  
 “ ”, “ ”, “
”, “ ”, “ ”, 
“ ” 
pH
.
5 6.5 ～
 7.5 9 ～
3 5～
 9 11 ～
3 
 11 
 16) 39) ( ) ∼ 
   (4) (18) ( ) ∼ 
3. 
 1) 14) ( ) ∼ 
 1 5 ) 
----------------------- 
-----------------------
-----------------------
----
  
 “ ”, “ ”, “
”, “”, “ ”, “
”, “ ”
---------- 
-------------------------
-------------------------
--------------
3
 7.5
 9
3 5
9
11
5 6.5
 11
6.5
7.5
 16) 39) ( ) ∼ 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 44 -
()
. Ⅱ
 1. 3.∼ 
 ( )
 4. 
  . 
Methyl Ethyl Cellulose
( )
< >
. Ⅱ
 1. 3.∼ 
 ( )
 4. 
  . 
Methyl Ethyl Cellulose
( )
(6S)-5-Methyltetrahydrofolic 
Acid, Glucosamine Salt
: C32H51N9O16
: 817.80
: 5-MTHF- 
glucosamineCAS No.: 
1181972-37
-1
  
3446%, ～
(6S)-5-methyltetrahydrofolic 
acid 5459∼
%
.
  
 ∼
.
. (1)
, 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 45 -
()
.
(1) : 5.0g
, 
2.0ppm .
(2) : 
, 
2.6ppm .
(3) : 
, 
0.1ppm .
(4) : 
 5.0g
, 1.0ppm 
 .
(5) : 2g
500mL 
20mL 30mL
10mL
. 2～
3mL ～
. 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 46 -
()
75mL 
25mL
50mL
. , 
. , 
「
」 
, 10ppm 
(, 
).
( 6 ) 
(6S)-5-Methyltetrahydrofolic 
acid : 
 35mg
90mL
20 1 ℃
100mL . 
5mL 10mL 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 47 -
()
10mL 0.45
m μ
.
25mg
(6R,S)-5-Methyltetrahydrofolic acid
calcium salt 90mL
20 1 ℃
100mL
. 5mL
10mL 
10mL
0.45
m μ
. 
, 
(6S)-5-Methyltetrahydrofolic
acid
 (%)99% 
. 
(6S)-5-Methyl
-
tetrahydrofoli
c acid(%) =(6S)-5-Methyltetr
ahydrofolic acid
×10
0(6S)-5-Methyltetr
ahydrofolic acid 
(6R)-5-Methyltetr
ahydrofolic acid 
  
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 48 -
()
  
 : 
 (
225nm)
  
 : Chiral HSA(Chromtech, 
4.0mm×100mm, 5m) μ
  
 : 30°C
  
 : 100mM NaH2PO4
10% NaOH pH 7.0
.
 940mL
2-propanol 60mL
.
  
 : 0.7mL/min
(6) : 
 「
」
(
) , 1g
100 .
(7) : 
 「
」 
, (-) .
(8) : 
 「
」 
(
) , 
1g 100 .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 49 -
()
(- )
, 8.0% 
.
(1) : 
0.5g 100mL 
: (75:25) 50mL
100mL
 . 0.45m μ
. 
 D-
0.5g
 : 
(75:25) 50mL
100mL
. 0.45
m μ
.
, 
.
(%) =Ac × Pstd × T% × 100
Astd × Pc × (100 - m)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 50 -
()
Ac :  
Pstd :  (mg)
Astd :  
Pc : (mg) 
T% : (%)
m : (%)
  
  
 : UV 195nm
  
 : Shodex Asahipak 
NH2P-50 4E(4.6mm x 250mm, 
5μm)
  
 : 35°C 
  
 : A : B = 75 : 25
  
 :
 A:
          
B:  
  
 : 1.0mL/min
(2) (6S)-5-Methyltetrahydrofolic 
acid
: 70mg
100mL
 0.45
m μ
.
(6S)-5- Methyltetrahydrofolic acid
calcium
(6S)-5-Methyltetrahydrofolic 
acid
 40mg
100mL 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 51 -
()
100mL
. 
, 
.
(%) =Ac × Pstd × T% × 100
Astd × Pc ×  (100 - m)
Ac :  
Pstd :  (mg)
Astd :  
Pc : (mg) 
T% : 
(6S)-5-Methyltetrahydrofolic acid (%)
m : (%)
  
  
 : UV 280nm
  
 :Gemini phenomenex 
(4.6mm×250mm, 5m) μ
  
 : 25°C 
  
 :
 A: KH2PO4 
6.8g
350mL
20% KOH pH 6.5
1000mL .
     
B: KH2PO4 
4.08g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 52 -
()
650mL , 
350mL
. 20% KOH
pH 8.0 .
  
()
 A(%)
 B(%)
0 100 0
15 60 40
17 30 70
22 30 70
31 100 0
  
 : 1.0mL/min
Cellulose, Powdered
( )
< >
Cellulose, Powdered
( )
Branching Glycosyltransferase
1,4--Glucan Branching α
Enzyme
  Rhodothermus
obamensis
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 53 -
()
Bacillus subtilis
. , 
, 
, 
. 
-1,4- α
-1,6- α
.
∼
, , 
. ∼
.
 (1) : 
, 
4.0ppm .
(2) : 
 5.0g
, 5.0ppm 
.
(3) : 「
」
, 1g30 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 54 -
()
.
(4) 
 : 「
」
, (-)
.
(5) 
 : 25g
「 」
, 
(-) .
()
: 
1-6-α 
, Branching 
Enzyme Units(BEU)
. 1 BEU
(pH 7.2, 60) ℃
660nm -
complex
.
: 1g(± 
0.5mg)
Tris-HCl 80mL
30
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 55 -
()
100mL 
Tris-HCl
100mL . 
Tris-HCl 
, 
3050 BEU/mL ∼
. (D)
(
1040 ). ∼
.
: 
.( 4
) 50Lμ
(Vs=0.050mL)50Lμ
, 
50L 50L μ μ
, 
100L . μ
6
0 30(t) ℃
, 30
2mL stop reagent
20
660nm . 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 56 -
()
4
(
:As, 
:AR
, 
:AB)
  ※ 
0.15
0.3 . ∼
 0.1M 
Tris-HCl(pH 7.2) 
.
  
.
(BEU/g) =(ARAs)×V×D×100 –
(ARA–B)×t×Vs×W
(AR
-As): 
(AR-AB
): 
V: (mL)
D: 
100: BEU/g
t: ()
Vs: 
(mL)
W: (g)
  
 : Branching 
Enzyme Units(BEU)
(pH 7.2, 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 57 -
()
60) 660nm ℃
- complex
, 1%
.
Enzymatically Modified 
Hesperidin
( )
< >
Enzymatically Modified 
Hesperidin
( )
Black Carrot Extract
: Black carrot 
colorINS No.: 
163(vi)
(Daucus carota L. ssp. 
sativus var. atrorubens Alef.)
, 
, 
(Anthocyanin). , 
, , 
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 58 -
()
  (Ecm
)
.
, , 
.
  
～
, 500550nm ～
.
 (1) 
 : 
, 
4.0ppm 
.
(2) : 5.0g
, 2.0ppm 
.
(3) : 5.0g
, 1.0ppm 
.
(4) : 0.1g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 59 -
()
, 
 1.0ppm 
.
(5) : 「 」
(3)
, 1 (Ecm
)
0.005% .
( )  
0.30.7～
pH 3.0
·
100mL
. 
. 
pH 3.0 ·
1cm, 500550nm ～
A
.
(Ecm)＝A × 10
(g)
  
 · (pH 3.0)
   
1 (0.1M ) : 
1L 21.01g
(C6H8O7H‧2
O) .
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 60 -
()
   
2 (0.2M 
) : 1L 71.63g
(Na2HPO412H‧ 2
O)
.
   
1 59 2 41
pH 3.0
.
Furcelleran
Furcellaria fastigata
 HUD.
.
 ( )
 ( )
 ( )
 ( )
 ( )
: Danish agarCAS No.: 
9000-21-9
Furcelleran
Furcellaria fastigata
 HUD.
( , 
, ) , 
------
 ( )
 ( )
 ( )
 ( )
 ( )
: Danish agarCAS No.: 
9000-21-9
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 61 -
()
Protease
( ), ( ), 
( ) . 
.
  
 ( ) : Aspergillus
niger
 , Aspergillus 
oryzae
. , , 
, 
.
  ( ) : Bacillus 
subtilis
 , Bacillus 
licheniformis 
 , 
Bacillus stearothermophilus 
, Bacillus 
amyloliquefaciens
. 
, , 
, 
.
  
 ( ) : , 
Protease
------------------- 
-----------------------
-----------------------
-----------------------.
  
------------------------
- - - 
--------------------------
---------------
 , 
Aspergillus melleus
 ----- 
-----------------------
----------------------.
  
-----------------------
- - 
-----------------------
-----------------------
-----------------------
-----------------------
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 62 -
()
,
. , 
, 
, 
.
  
.
. 
 ( ) [Protease, 
Fungal]
 ( )
 ( )
 ( )
( )
 (
)
1 
SAP(Spectrometric acid 
protease units) 2 
HUT(Hemoglobin units on 
the tyrosine basis)
. 
  
< >
1 SAP(Spectrometric acid 
protease units)
 ( )-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
---------------.
. 
 ( ) [Protease, 
Fungal]
 ( )
 ( )
 ( )
( )
------------ 
-----------------------
-----------------------
-----------------------
-----------------------
-----------------------
---------------.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 63 -
()
2 HUT(Hemoglobin units on 
the tyrosine basis)
 ( )
 ( )
. 
 ( ) [Protease,
Bacterial]
 ( )
. ( ) [Plant 
Protease]
 ( )  
, Aspergillus melleus
2 
.
1 SAP(Spectrometric acid 
protease units)
 ( )
2 HUT(Hemoglobin units on 
the tyrosine basis)
(
)
 ( )
. 
 ( ) [Protease,
Bacterial]
 ( )
. ( ) [Plant 
Protease]
 ( )
5. 
 . 
  
. , 
II. 2. 1)
.
5. 
 . 
  ----------------------- 
-----------------------
-----------------------
-----------------------
-----------------------
----------------.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 64 -
()
.
 1. 4. () ∼ 
 5. 
 , 
, ․  : 
0.150g/kg(, 5
)
< >
6. 
  : 
1.0g/kg
(, 「
」
(
)
 「
(
) (」
)
( )
, 
2.0g/kg, 
0.20g/kg)
7. 
 : 
0.030g/kg
()
---------------
- 
---------------
---------------
---------------
---------------
----
1. 4. ( ∼ 
)
 5. 
 , 
 : 
---------- 
---------------
---------------
---------------
----------
6. 
 · : 
0.030g/kg(, 5
0.150g/kg)
7. 
  : ---- 
----------------
----------------
----------------
----------------
----------------
----------------
----------------
--------------
------------- 
----------------
----------------
----------------
----------------
----------------
----------------
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 65 -
()
8. ∼ 11
. ()
12. 
  : 
0.10g/kg
13. ∼ 15
. ()
16. 
 [, 
, , , 
(
, ), 
] : 
0.030g/kg
.
 1. 4. () ∼ 
 5. 
 , 
()
-
8. , 
: 0.50g/kg
(, 「 」
( )
「
( )
(」
)
·
( )
)
9. ∼ 12
. (
)
13. 
 : --------
14. ∼ 16
. (
)
17. 
 (
, ), 
,
 , 
, , ․
, , 
, , , 
, , 
( , 
), 
, , 
, , , 
, , 
: 0.030g/kg
---------------
- 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 66 -
()
, ․  : 
0.150g/kg(, 5
)
< >
6. 
  : 
1.0g/kg
(, 「
」
(
)
 「
(
) (」
)
( )
, 
2.0g/kg, 
0.20g/kg)
7. 
 : 
0.030g/kg
---------------
---------------
---------------
---------------
----
1. 4. ( ∼ 
)
 5. 
 , 
 : 
---------- 
---------------
---------------
---------------
----------
6. 
 · : 
0.030g/kg(, 5
0.150g/kg)
7. 
  : ---- 
----------------
----------------
----------------
----------------
----------------
----------------
----------------
--------------
------------- 
----------------
----------------
----------------
----------------
----------------
----------------
-
8. , 
: 0.50g/kg
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 67 -
()
8. ∼ 11
. ()
12. 
  : 
0.10g/kg
13. ∼ 15
. ()
16. 
 [, 
, , , 
(
, ), 
] : 
0.030g/kg
< >
 < >
 < >
.
 1. 4. () ∼ 
 5. 
 , 
, ․  : 
()
(, 「 」
( )
「
( )
(」
)
·
( )
)
9. ∼ 12
. (
)
13. 
 : --------
14. ∼ 16
. (
)
17. 
 (
, ), 
,
 , 
, , ․
, , 
, , , 
, , 
( , 
), 
, , 
, , , 
, , 
: 0.030g/kg
.
 1. 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 68 -
()
0.150g/kg(, 5
)
< >
6. 
  : 
1.0g/kg
(, 「
」
(
)
 「
(
) (」
)
( )
, 
2.0g/kg, 
0.20g/kg)
7. 
 : 
0.030g/kg
---------------
- 
---------------
---------------
---------------
---------------
----
1. 4. ( ∼ 
)
 5. 
 , 
 : 
---------- 
---------------
---------------
---------------
----------
6. 
 · : 
0.030g/kg(, 5
0.150g/kg)
7. 
  : ---- 
----------------
----------------
----------------
----------------
----------------
----------------
----------------
--------------
------------- 
----------------
----------------
----------------
----------------
----------------
----------------
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 69 -
()
8. ∼ 11
. ()
12. 
  : 
0.10g/kg
13. ∼ 15
. ()
16. 
 [, 
, , , 
(
, ), 
] : 
0.030g/kg
< >
 < >
 < >
.
 1. 4. () ∼ 
 5. 
 , 
, ․  : 
0.150g/kg(, 5
()
-
8. , 
: 0.50g/kg
(, 「 」
( )
「
( )
(」
)
·
( )
)
9. ∼ 12
. (
)
13. 
 : --------
14. ∼ 16
. (
)
17. 
 (
, ), 
,
 , 
, , ․
, , 
, , , 
, , 
( , 
), 
, , 
, , , 
, , 
: 0.030g/kg
II. 2. 1)
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 70 -
()
)
< >
6. 
  : 
1.0g/kg
(, 「
」
(
)
 「
(
) (」
)
( )
, 
2.0g/kg, 
0.20g/kg)
7. 
 : 
0.030g/kg
8. ∼ 11
. ()
---------------
- 
---------------
---------------
---------------
---------------
----
1. 4. ( ∼ 
)
 5. 
 , 
 : 
---------- 
---------------
---------------
---------------
----------
6. 
 · : 
0.030g/kg(, 5
0.150g/kg)
7. 
  : ---- 
----------------
----------------
----------------
----------------
----------------
----------------
----------------
--------------
------------- 
----------------
----------------
----------------
----------------
----------------
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 71 -
()
12. 
  : 
0.10g/kg
13. ∼ 15
. ()
16. 
 [, 
, , , 
(
, ), 
] : 
0.030g/kg
. 
 1. 3. () ∼ 
 4. (, 
8% 
), , 
, , 
, , 
, (, 
), 
, , 
, 
( ), 
, 
1.0g/kg ∶
()
----------------
-
8. , 
: 0.50g/kg
(, 「 」
( )
「
( )
(」
)
·
( )
)
9. ∼ 12
. (
)
13. 
 : --------
14. ∼ 16
. (
)
17. 
 (
, ), 
,
 , 
, , ․
, , 
, , , 
, , 
( , 
), 
, , 
, , , 
, , 
: 0.030g/kg
-----------------
-----------------
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 72 -
()
< >
 5. ()
 6. 
  : 
1.0g/kg 
( , 
,
1.0g/kg 
, 
0.6g/kg )
< >
7
. ()
-----------------
-----------------
--
1. 3. ( ∼ 
)
 4 . 
------------- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---, 
 , 
-------- ∶
(, ,  
1.0g/kg 
, 
0.2g/kg )
 5. ( )
 6. 
 , ·
 : 
------------ 
-----------------
-----------------
-----------------
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 73 -
()
8. 
 , 
, (
) : 
0.5g/kg 
9.∼16
. ()
. 
 1. 3. () ∼ 
 4. (, 
8% 
), , 
, , 
, , 
, (, 
), 
, , 
, 
( ), 
, 
1.0g/kg ∶
< >
 5. ()
 6. 
  : 
()
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
---------------
7. 
 : 
0.5g/kg
 (
, , 
0.6g/kg , 
0.5g/kg 
)
8
. ()
9. , 
----- 
----------------
----------------
------
10.∼17.
 (
)
-----------------
-----------------
-----------------
-----------------
----------
1. 3. ( ∼ 
)
 4 . 
------------- 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 74 -
()
1.0g/kg 
( , 
,
1.0g/kg 
, 
0.6g/kg )
< >
7
. ()
8. 
 , 
, (
) : 
0.5g/kg 
9.∼16
. ()
. 
 1. 3. () ∼ 
 4. (, 
8% 
()
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---, 
 , 
-------- ∶
(, ,
1.0g/kg 
, 
0.2g/kg )
 5. ( )
 6. 
 , ·
 : 
------------ 
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
---------------
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 75 -
()
), , 
, , 
, , 
, (, 
), 
, , 
, 
( ), 
, 
1.0g/kg ∶
< >
 5. ()
 6. 
  : 
1.0g/kg 
( , 
,
1.0g/kg 
, 
0.6g/kg )
< >
7. 
 : 
0.5g/kg
 (
, , 
0.6g/kg , 
0.5g/kg 
)
8
. ( )
9. , 
- - - - 
---------------
---------------
------------
10.∼17.
(
)
-----------------
-----------------
-----------------
-----------------
-----------
1. 3. ( ∼ 
)
 4 . 
------------- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 76 -
()
7
. ()
8. 
 , 
, (
) : 
0.5g/kg 
9.∼16
. ()
.
 1. 4. () ∼ 
 5. 
 , 
, ․  : 
0.150g/kg(, 5
)
< >
6. 
  : 
1.0g/kg
(, 「
」
(
)
 「
(
()
---------------
---------------
---------------
---, 
 , 
-------- ∶
(, ,
1.0g/kg 
, 
0.2g/kg )
 5. ( )
 6. 
 , ·
 : 
------------ 
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
-----------------
---------------
7. 
 : 
0.5g/kg
 (
, , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 77 -
()
) (」
)
( )
, 
2.0g/kg, 
0.20g/kg)
7. 
 : 
0.030g/kg
8. ∼ 11
. ()
12. 
  : 
0.10g/kg
13. ∼ 15
. ()
16. 
 [, 
, , , 
(
, ), 
] : 
0.030g/kg
0.6g/kg , 
0.5g/kg 
)
8
. ( )
9. , 
- - - - 
---------------
---------------
------------
10.∼17.
(
)
---------------
- 
---------------
---------------
---------------
---------------
----
1. 4. ( ∼ 
)
 5. 
 , 
 : 
---------- 
---------------
---------------
---------------
----------
6. 
 · : 
0.030g/kg(, 5
0.150g/kg)
7. 
  : ---- 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 78 -
()
. 
 1. ·
( ) 
0.6g/kg ( ∶
,
, 
1.0g/kg 
, 
0.6g/kg )
 2. 
 (
) : 0.6g/kg 
< >
()
----------------
----------------
----------------
----------------
----------------
----------------
----------------
--------------
------------- 
----------------
----------------
----------------
----------------
----------------
----------------
-
8. , 
: 0.50g/kg
(, 「 」
( )
「
( )
(」
)
·
( )
)
9. ∼ 12
. (
)
13. 
 : --------
14. ∼ 16
. (
)
17. 
 (
, ), 
,
 , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 79 -
()
 3.11. () ∼
. 
 1. ·
( ) 
0.6g/kg ( ∶
,
, 
1.0g/kg 
, 
0.6g/kg )
 2. 
 (
) : 0.6g/kg 
< >
 3.11.() ∼
()
. 
()
, , ․
, , 
, , , 
, , 
( , 
), 
, , 
, , , 
, , 
: 0.030g/kg
 ---------------- 
----------------
----------------
----------------
-------------
 1 . 
-------------- 
---------------
---------------
-- 
 , ·
---------------
- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
------
 2. 
  : 
------ --------
( , 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 80 -
()
 1. ·
( ) 
0.6g/kg ( ∶
,
, 
1.0g/kg 
, 
0.6g/kg )
 2. 
 (
) : 0.6g/kg 
< >
 3.11.() ∼
. 
 1. ·
( ) 
0.6g/kg ( ∶
,
()
0.6g/kg , 
0.5g/kg )
 3.11. ∼
 (
)
 ---------------- 
----------------
----------------
----------------
----------------
---
 1 . 
-------------- 
---------------
---------------
-- 
 , ·
---------------
- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
------
 2. 
  : 
------ --------
( , 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 81 -
()
, 
1.0g/kg 
, 
0.6g/kg )
 2. 
 (
) : 0.6g/kg 
< >
 3.11.() ∼
5'
-
  5'-
. 5'-
 1. ()
< >
()
. 
 1.2. () ∼
 3. 
 , 
()
0.6g/kg , 
0.5g/kg )
 3.11.(∼
)
 ---------------- 
----------------
----------------
----------------
----------------
---
 1 . 
-------------- 
---------------
---------------
-- 
 , ·
---------------
- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
------
 2. 
  : 
------ --------
( , 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 82 -
()
0.02% ：
( )
 4. 
 · (
)
...
.
 ....
. ....
...
.
 1. 5. () ∼
 6. 
 (
) : 
0.265g/kg (.
...
...
.
0.265g/kg )
 7.8. () ∼
()
...
.
 ....
. ....
....
 1.5. () ∼
 6. 
 (
) : 
0.265g/kg (.
...
...
()
0.6g/kg , 
0.5g/kg )
 3.11.(∼
)
 ---------------- 
----------------
----------------
----------------
----------------
---
 1 . 
-------------- 
---------------
---------------
-- 
 , ·
---------------
- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
------
 2. 
  : 
------ --------
( , 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 83 -
()
.
0.265g/kg )
 7.8. () ∼
.
 1. 4. () ∼ 
 5. 
 , 
, ․  : 
0.150g/kg(, 5
)
< >
6. 
  : 
1.0g/kg
(, 「
」
(
)
 「
(
) (」
)
( )
, 
2.0g/kg, 
0.20g/kg)
()
0.6g/kg , 
0.5g/kg )
 3.11.(∼
)
5'
-
---------------
- 
---------------
---------------
---------------
---------------
--
 1. ( )
2. 
 : 8g/kg 
(
)
---------------
- 
---------------
---------------
------
 1.2. ( ∼
)
 3. 
 , 
- - - - 
---------------
----------
 4. 
--------- 
-------------
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 84 -
()
7. 
 : 
0.030g/kg
8. ∼ 11
. ()
12. 
  : 
0.10g/kg
13. ∼ 15
. ()
16. 
 [, 
, , , 
(
, ), 
] : 
0.030g/kg
()
...
.---------------
- 
---------------
---------------
---------------
---------------
---------------
---------------
-
 1.5. ( ∼
)
 6. 
----- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
----
 7.8. ( ∼
)
(
)
...
.
---------------
- 
---------------
---------------
---------------
---------------
---------------
---------------
-
 1.5. ( ∼
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 85 -
()
. 
 1.6. () ∼
 7. 
 : 0.2g/kg 
< >
 8. 
 (
 ) : 
0.012g/kg 
 9. ()
. 
 1.6. () ∼
 7. 
 : 0.2g/kg 
< >
)
 6. 
----- 
---------------
---------------
---------------
---------------
---------------
---------------
---------------
---------------
----
 7.8. ( ∼
)
---------------
- 
---------------
---------------
---------------
---------------
----
1. 4. ( ∼ 
)
 5. 
 , 
 : 
---------- 
---------------
---------------
---------------
----------
6. 
 · : 
0.030g/kg(, 5
0.150g/kg)
7. 
  : ---- 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 86 -
()
 8. 
 (
 ) : 
0.012g/kg 
 9. ()
 1.3. () ∼
 4. 
 : 2% 
< >
()
< >
 < >
 < >
----------------
----------------
----------------
----------------
----------------
----------------
----------------
--------------
------------- 
----------------
----------------
----------------
----------------
----------------
----------------
-
8. , 
: 0.50g/kg
(, 「 」
( )
「
( )
(」
)
·
( )
)
9. ∼ 12
. (
)
13. 
 : --------
14. ∼ 16
. (
)
17. 
 (
, ), 
,
 , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 87 -
()
, , ․
, , 
, , , 
, , 
( , 
), 
, , 
, , , 
, , 
: 0.030g/kg
---------------
- 
---------------
---------------
---------------
---------------
---------------
---
 1.6. ( ∼
)
 7. --- : 
-------- 
( , 
1.0g/kg 
, 
0.2g/kg 
)
 8. 
-------- 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 88 -
()
---------------
------
 9. ( )
---------------
- 
---------------
---------------
---------------
---------------
---------------
---
 1.6. ( ∼
)
 7. --- : 
-------- 
( , 
1.0g/kg 
, 
0.2g/kg 
)
 8. 
-------- 
---------------
------
 9. ( )
(
)
 1.3. () ∼
 4. 
 : 2% 
(
)
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 89 -
()
(, 
0.3% )
.
1. 
 . 
  ( )
  
 . , , 
, ·
, · , ·
  II. 5. .
,
, , 
, ․ ․
, ․
( “ ” )
.
  (1) ( )
  (2) 
 . 
  ( )
  
 . , , 
, ·
, · , ·
  
------------------------
- - 
------------------------
------------------------
------------------------
------------------------
------------------------
------------------------
------------------------
------------------------
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 90 -
()
.
( )
(Guar Gum)
(Citric Acid)
(Trisodium Citrate)
(Potassium Citrate)
(Glucoamylase)
(Glycerin Esters of 
Fatty Acids)
(Lactoferrin Concentrates)
(Lecithin)
(Locust Bean Gum)
(Lysozyme)
(Maltogenic Amylase)
(Mucin)
(Vanillin) 
(Food Starch Modified)
< >
(Calcium Hydroxide)
(Arabic Gum)
- ( )(-Amylase, α α
Nonbacterial)
- ( )(-Amylase, α α
Bacterial)
L- (Calcium L-Ascorbate)
L- (L-Ascorbyl Palmitate)
(Ethyl Vanillin)
(Magnesium Chloride)
(Lactic Acid)
(Potassium Phosphate, Dibasic)
(Gelatin)
(Carrageenan)
(Casein)----------------
  (1) ( )
  ( 2 ) 
---------------------- 
-----------------------
-----------------------
--
( )
------------------
------------------
--------------------------
--------------------------
--------------------------
---------------------------------
-------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
(Potassium Hydroxide)
--------------------------
--------------------------
---------------------------------
-------------------
--------------------------
--------------------------
--------------------------
--------------------------
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 91 -
()
(Sodium Caseinate)
(Sodium Carbonate)
(Sodium Bicarbonate)
(Potassium Bicarbonate)
()(Potassium Carbonate, 
Anhydrous)
d
- ( )(d-Tocopherol 
concentrate, Mixed)
(Pectin)
( )(Plant Protease)
(Heme Iron)--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
--------------------------
---------------------------------
-------------------
---------------------------------
-------------------
--------------------------
--------------------------
--------------------------
IV. 
 1. 7.∼ 
 ( )
8. 
. 
< >
IV. 
 1. 7.∼ 
 ( )
8. 
. 
1) 
  
. , 
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 92 -
()
  
(1) 1
   
 5mL , 
.
5‘- , 
, , 
, , L-
, , 
, , 
L- , L- , 
L- , 5'-
, D- , 
, D- , 
, , 
, DL- , DL-
, 
, L- , , 
, D- , D-
, , 5'-
, L- , 
L- , 
, , DL-
, L- , 
, , 
, , 
, , 5'-
, 5’-
, , 
, , 
, 
, , 
, , 
, 
, DL- , L-
, 
, , 
, , 
, , L-
, , 
, , 0.25g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 93 -
()
, 
, L- , 
, , 
, , , 
, , 
, L- , L-
0.33g
L- 0.4g
, 
0.5g
, , 
, 0.77g
D- 1g
, 
0.25g
(200, 4hr ℃
)
-- , δ
, L- , , 
, ....
, ....
, DL-
, L- , 
, DL-
, , 
, 
0.25g, 
10mL
0.25g, 
25mL
0.38g, 
20mL
0.39g(
),
20mL
0.77g, 
10mL
0.25g, 
10mL
5mL, 
50mL
5mL 
, 
100mL
25mL 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 94 -
()
  
  
(2) 2
   
, , 
(1
50) 10mL →
450550 . ∼ ℃
450550 . ∼ ℃
 3mL
0.5g, 5mL
10mL
5mL 
0.66g, 
10mL
5mL 
10g, 
200mL
5mL 
, 
100mL
12.5mL 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 95 -
()
.
, , , 
, , , 
, , , -β
, , 
, - , α
, 
, , 
, , 
L- , 
, , 
, , 
, , 
, , 
5'- , 
, , , 
, , , 
, , , 
, , 
, , 
- , β
, , dl-
, l- , , 
, , 
, , , , 
, 
, , 
, , 
B2
 , 
C, E, , 
, , 
, 
, , , 
, 
, , 
, L- , 
, , 
, , 
, , , 
- , - α α
, L-
, L-
, L-
, , L-0.25g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 96 -
()
, L- , 
, -β
-8'- , , 
, , 
, , 
, , 
, , 
, 
, , 
, , 
, , 
, 
, - , γ
, , , 
, , 
, , , 
, , 
, 
, , 
, , 
, , 
, , 
, DL- , L- , 
, , 
, , 
, , , 
, , 
, , , 
- , , β
, 
, 
, , 
, , , 
, , , 
, , 
, , , , 
, , 
, 
, d- ( ), 
, 
, , 
, L- , 
, 
, , 
, , 
, , , , 
, , 
, , 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 97 -
()
  (3)
.
()
0.25g 5mL
15mL
.
0.25mL
10mL
, 
, , -ε
, , 
20, 60, 
65, 
80, , 
, 
, , , 
, , , 
, 
, , 
, , , 
, , , 
, 
, 0.38g
, , 
, 
, , , 
, , 
, , , 
, , 
, , dl-α
-
, , 
, , 
, 0.5g
, , , 
, , 
, 0.77g
, 1g
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 98 -
()
.
0.5g 5mL
1mL 10mL
2mL
10mL 5mL
.
0.5g 5mL, 
1mL 10mL
2mL 
10mL 
5mL
.
0.77g 5mL
.
2.5g250mL 
0.5N 50mL
. 30
No. 
3 . 
10mL 3
100mL 10mL
.
5g 50mL
50 15 ℃
. 
100mL
2mL
.
0.25g 20mL
.
1.25g
10mL 10mL
. 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 99 -
()
2mL . 
～
. 
15mL
. 
25mL
5mL
.
0.2g , 
5mL
. 
5mL
.
0.5g 
0.3g ․
1mL
400℃
. 
2mL ․
10mL
5mL
.
5‘-
5'-
5'-
0.25g 5mL
.
0.25g
150mL 10mL
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 100 -
()
10mL 5mL
5mL
10mL
. 
10mL
.
2.5g
25mL
5mL 1mL
2mL
10mL
5mL
.
5g
100mL
5mL (1
4)→
5mL
.
DL-
L-
0.5g500mL 
5mL 
5mL
23mL～
～
. 
15mL
23mL . ～
10mL
5mL
.
0.25g 5mL
30
5mL , 
.
0.5g 5mL
15
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 101 -
()
70℃
. 
5mL 10mL
20mL 10mL
.
0.77g 10mL
15
70℃
. 
10mL, 20mL
40mL
20mL
.
A
1g
20mL
. 5mL
. 
5mL
. 
～
. 
15mL
. 
20mL
5mL
.
B2
.
1.25g
10mL 5mL
. 
～
. 10mL 
15mL
. 
25mL 5mL
.
2.5g250mL 
(125) 100mL →
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 102 -
()
15
. 
200mL 
(1
25) 25mL 4 →
(125)→
200mL
20mL
.
0.25g (12) →
10mL
25mL
.
0.25g (14) →
5mL
.
0.25g 15mL
.
1g (12) 30mL →
1mL
5mL 15mL
3
50mL
. 25mL
1mL
10mL
2mL
5mL
.
0.25g 5mL
2mL
.
50g
250mL
. 1.3mL
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 103 -
()
5mL
.
20%
. 
1.3mL 5mL
.
0.25g 5mL
.
0.25g (12) 5mL →
.
0.25g
.
L-
1g100mL 
5mL 
5mL . 
23mL～
～
. 
15mL
23mL . ～
10mL
5mL .
1g 5mL 
1mL
10mL
2mL
5mL
.
10g250mL 
0.5N 50mL
. 
15
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 104 -
()
No. 4 
. 
10mL 4
100mL
2.5mL
.
0.25g 5mL
2mL
5mL
.
2mL 250mL
(A)
. 1mLCl2
 1g
. (A) 10mL
100mL
2.5mL
.
0.5g 20mL
L- 0.2g
.
5g 30mL
(120) →
6mL
50mL
5mL
.
L-
0.25g1N 10mL
.
10.0g
0.5N 
50mL
15 . 
100150mL ～
1015～
(Whatman No. 4 
)
100mL . 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 105 -
()
1015mL ～
. 2
100mL 2.5mL
.
10g250mL
(120) 50mL →
15
10mL3
10～
15mL , 
100mL 7.5mL
.
0.25g500mL 
5mL 
5mL
. 23mL～
～
. 
15mL
2～
3mL
.
L-
0.25g500mL 
5mL 
5mL
. 23mL∼
∼
. 
15mL
2∼
3mL
.
0.5g 25mL
1mL 
10mL 2mL
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 106 -
()
10mL 5mL
.
0.5g
10mL , 1mL 
10mL 2mL
10mL
5mL
.
0.5g 10mL
, 1mL 10mL
2mL
10mL 5mL
.
0.25g
10mL
.
10g
(14) 60mL →
15mL
1.5g
30mL 1
. 1
3 10mL
5
(14) 50mL →
5mL
.
2.5g 10mL
5mL
2mL
50mL . 5mL
1mL
5mL
.
2.5g 25mL
5mL 1mL
2mL
10mL 5mL
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 107 -
()
.
0.25g
5mL 
5mL
. 23mL～
～
. 
, 15mL
23mL～
, 
.
0.25g 5mL
. 1mL
.
()
1g 10mL
2mL
20mL
5mL
.
0.25g (1→
4) 5mL
. 
(14) 5mL →
10mL
.
DL-
L-
0.25g1N 3mL 
2mL
.
5g 50mL
50 15 ℃
. 
100mL 2mL
.
L-
 0.25g2N 10mL
.
0.25g(, 
0.25g )
5mL 
5mL
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 108 -
()
. 2～
3mL ～
. 
15mL
2～
3mL
.
1g 10mL, 
10mL
30mL
100mL 
100mL 25mL
.
12.5mL
5mL, 
1mL 10mL
2mL
, 
5mL
.
0.25g 5mL
2mL 1.5g
5 L-
0.2g
.
1g 6mL 
30mL
40mL 10mL
.
2g500mL 
20mL 
30mL
10mL
23mL～
～
. 75mL 
25mL
. 
50mL
, 100mL 
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21- 109 -
()
1)∼ 5)
 ( )
. 
  ( )
2)∼ 6)
 ( )
. 
  ( )
100mL . 
20mL
. , 
, 5mL
100mL
 20mL
. , 
6mL
. 
, 3mL
.
0.5g
5mL 
5mL . 
23mL～
～
. , 
15mL
23mL , ～
.
이상엽 / 세계무역기구과 / 2016-12-29 17:36:21