COMMI SSION IMPLEMENTING REGUL ATION (EU) 2017/194 
of 3 Febr uary 2017 
concer ning the author isation of the preparation of Lactobacillus diolivo rans DSM 32074 as a feed 
additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Having regar d to the Treaty on the Functioning of the European Union, 
Having regar d to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 
on additives for use in animal nutr ition (1), and in particular Article 9(2) thereof, 
Whereas: 
(1)  Regulation (EC) No 1831/2003 provides for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitte d for the author is­
ation of the preparation of Lactobacillus diolivor ans DSM 32074. This application was accompanied by the 
particulars and documents required under Article 7(3) of Regulation (EC) No 1831/2003. 
(3)  This application concer ns the author isation of the preparation of Lactobacillus diolivo rans DSM 32074 as a feed 
additive for all animal species to be classifie d in the catego ry ‘technological additives ’. 
(4)  The European Food Safety Auth ority (‘the Authority’) concluded in its opinion of 12 July 2016 (2) that, under the 
proposed conditions of use, the preparation of Lactobacillus dioliv orans DSM 32074 does not have an adverse 
effect on animal health, human health or the envi ronment. However , the additive should be considered to have 
the potential to be a respirator y sensitiser . The Author ity also concluded that the concer ned preparation has the 
poten tial to impro ve the production of silage prepared from easy , moderately difficult and difficult to ensile 
mate rial. The Author ity does not consider that there is a need for specifi c requirements of post-market 
monitoring. It also verified the repor t on the methods of analysis of the feed additive in feed submitted by the 
Reference Laboratory set up by Regulation (EC) No 1831/2003. 
(5) The assessment of the preparation of Lactobacillus diolivor ans DSM 32074 shows that the conditions for author is­
ation, as provided for in Article 5 of Regulation (EC) No 1831/2003, are satisfi ed. According ly, the use of that 
preparation should be author ised as specif ied in the Annex to this Regulation. 
(6)  The measures provided for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed, 
HAS ADOPTED THIS REGUL ATION: 
Article 1 
Author isation 
The preparation specifi ed in the Annex, belonging to the additive catego ry ‘technological additives ’ and to the functional 
group ‘silag e additives’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that 
Annex. 4.2.2017 L 31/18 Official Jour nal of the European Union EN     
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Journal 2016; 14(9):4556. Article 2 
Entr y into force 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the 
European Union. 
This Regulation shall be binding in its entirety and directly applicable in all Member State s. 
Done at Brussels, 3 Febr uary 2017. 
For the Commission 
The Presid ent 
Jean-Claude JUNCKER  4.2.2017 L 31/19 Official Jour nal of the European Union EN     ANNEX 
Identifica ­
tion 
number of 
the additive Additive Chemical formula, descr iption, methods 
of analysis Species or 
categor y of 
animal Maximum 
age Minimum 
content Maximum 
conten t 
Other provisions End of period 
of author isation CFU of additive/kg of fresh 
material 
Technological additiv es: silage additiv es 
1k20752 Lactobacillus 
diolivo rans 
DSM 32074 Additive composition 
Preparation of Lactobacillus dioliv orans 
DSM 32074 containing a minimum 
of 3 × 1011 CFU/g additive. 
Char acter isation of the activ e substance 
Viable cells of Lactobacillus dioliv orans 
DSM 32074. 
Analytical method (1) 
Enumeration in the feed additive: 
spread plate method on MRS agar 
(EN 15787). 
Identif ication of the feed additive: 
Pulsed Field Gel Electrophoresis 
(PFGE). All animal 
species — — — 1.  In the directions for use of the additive 
and premixture, indicate the storage 
conditions. 
2.  Minimum cont ent of the additive when 
used without combination with other 
micro-org anisms as silage additives: 
1 × 108 CFU/kg fresh mat erial. 
3.For users of the additive and premix ­
tures, feed business operators shall es­
tablish operational procedures and or­
ganisational measures to address poten ­
tial risks resulting from its use. Where 
those risks cannot be eliminated or re­
duced to a minimum by such proce ­
dures and measures, the additive and 
premixtures shall be used with personal 
prote ctive equipment, including breath ­
ing prote ction. 24 Febr uary 
2027 
(1)  Details of the analytical methods are available at the following address of the Reference Laboratory : http://ir mm.jrc.ec.europa.eu/EURLs/EURL_feed _additives/Pag es/index.aspx   4.2.2017 L 31/20 Official Jour nal of the European Union EN