COMMISSION IMPLEMENTING REGUL ATION (EU) No 852/2014 
of 5 August 2014 
concer ning the author isation of L-methionine as a feed additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Having regar d to the Treaty on the Functioning of the European Union, 
Having regar d to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 
on additives for use in animal nutr ition (1), and in particular Article 9(2) thereof, 
Whereas: 
(1)  Regulation (EC) No 1831/2003 provides for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitt ed for the author isa­
tion of L-met hionine as a feed additive. That application was accompanied by the particulars and documents 
required under Article 7(3) of Regulation (EC) No 1831/2003. 
(3)  That application concer ns the author isation of L-methionine produced by Escher ichia coli (KCCM 11252P and 
KCCM 11340P) as a feed additive for all animal species, to be classifie d in the additive catego ry ‘nutr itional 
additives’. 
(4)  The European Food Safety Author ity (‘the Auth ority’) concluded in its opinion of 8 Octobe r 2013 (2) that, under 
the proposed conditions of use, L-methionine produced by Escher ichia coli (KCCM 11252P and KCCM 11340P) 
does not have an adverse effect on animal health, human health or the environment and that it may be consid ­
ered efficacious source of the amino acid L-met hionine for all animal species. The Author ity does not consider 
that there is a need for specific requirements of post-market monitori ng. It also verified the repor t on the method 
of analysis of the feed additive in feed submitted by the Reference Laboratory set up by Regulation (EC) 
No 1831/2003. 
(5) The assessment of that substance shows that the conditions for author isation, provided for in Article 5 of Regu ­
lation (EC) No 1831/2003, are satisfied. According ly, the use of that substance should be author ised, as specified 
in the Annex to this Regulation. 
(6)  The Auth ority expressed in its opinion concer ns over the safety of L-methionine for the target species when 
administ ered via wate r for drinking. However , no maximum content for L-met hionine is proposed by the 
Author ity. Thus, it is in the case of administration of L-methionine via drinking water appropr iate to instr uct the 
user to consider all diffe rent methionine sources in order to achi eve an optimal supply with the essential amino 
acids without affecting the performa nce of the animals. 
(7)  The measures provided for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed, 
HAS ADOPTED THIS REGUL ATION: 
Article 1 
The substance specifi ed in the Annex, belonging to the additive categor y ‘nutr itional additives ’ and to the functional 
group ‘amino acids, their salts and analogues’, is author ised as an additive in animal nutr ition subject to the conditions 
laid down in that Annex. 6.8.2014 L 233/22 Official Jour nal of the European Union EN     
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Journal 2013; 11(10):3428. Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the 
European Union. 
This Regulation shall be binding in its entirety and directly applicable in all Member State s. 
Done at Brussels, 5 August 2014. 
For the Commission 
The Presid ent 
José Manuel BARR OSO  6.8.2014 L 233/23 Official Jour nal of the European Union EN     ANNEX 
Identificat ion 
number of 
the additive Name of the 
holder of 
author isation Additive Composition, chem ical formula, descr iption, analytical 
method. Species or 
categ ory 
of animal Maxi ­
mum 
age Minimum 
content Maximum 
conten t 
Other provisions End of 
period of 
author isation mg/kg of complet e feed with 
a moisture conten t of 12 % 
Categor y of nutr itional additiv es. Functional group: amino acids, their salts and analogues 
3c305 — L-methio ­
nine Additive composition 
L-methionine with a purity of at least 98,5 % 
Char acter isation of the activ e substance 
L-methionine ((2S)-2-amino-4-(methy lthio) buta ­
noic acid) produced by fermentation with Escher i­
chia coli (KCCM 11252P and KCCM 11340P) 
Chemical formula: C5H11NO2S 
CAS number : 63-68-3 
Analytical method (1) 
For the identif ication of L-met hionine in the feed 
additive: infrared absor ption and optical rotation 
— FCC monographs methods. 
For the quantifi cation of methionine in the feed 
additive and premixtures containing more than 
10 % of methionine: ion exchange chromat o­
graphy coupled with post-column derivatisation 
and phot ometr ic or fluorescence detec tion 
(HPLC- VIS/FD) — ISO/DIS 17180. 
For the determination of methionine in premix ­
tures containing less than 10 % of methionine, 
compound feed, feed mater ials and water: ion 
exchang e chromat ography coupled with post- 
column derivatisation and photome tric detection 
(HPLC/VIS) — Commission Regulation (EC) 
No 152/2009 (2) (Annex III, F). All 
species —   1.  L-met hionine can be 
also used via water for 
drinking. 
2.  Declarations to be made 
on the labelling of the 
additive and premix ­
tures: 
‘If the additive is admi ­
niste red via water for 
drinking, excess prote in 
should be avoided.’ 
3.Where voluntar y declar ­
ation of the additive is 
made on the labelling of 
feed mate rials and 
compo und feed, the 
following shall be 
included: 
—name and identif ica­
tion number of the 
additive, 
—  added amount of the 
additive. 26 August 
2024 
(1)  Details of the analytical methods are available at the following address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts 
(2)  OJ L 54, 26.2.2009, p. 1.   6.8.2014 L 233/24 Official Jour nal of the European Union EN