PUBLIC REVIEW 
DRAFT  DRAFT UGANDA STANDARD  
  DUS 1577  
First Edition  
2014 -mm-dd 
 
 
 Reference number  
DUS 1577: 201 4 
© UNBS 2014 
  
Biopesticide ─ Specification  
 DUS 1577: 2014 
ii © UNBS 2014 – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2014  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS . 
Requests for permission to reproduce this document should be addressed to 
The Executive Director  
Uganda National Bureau of Standards  
P.O. Box 6329 
Kampala  
Uganda  
Tel: 256 414  505 995 
Fax: 256 414  286 123  
E-mail: info@unbs.go.ug  
Web: www.unbs.go.ug  
 
. DUS 1577 :2014 
© UNBS 2014 – All rights reserved  iii 
 Foreword  
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade,  Industry and 
Cooperatives established under Cap 327, of the Laws of Uganda.  UNBS is mandated to co-ordinate the 
elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT/SPS Agreements of the World Trade Organisat ion (WTO).  
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of representatives of 
consumers, traders, academicians, manufacturers, government and other stakeholders.  
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
This Draft Uganda Standard has been developed as a result of a need to provide guidance to industry in production and regulation of Biopesticides . 
This Draft Uganda Standard was developed by the UNBS/TC 2/ SC 20 – Agrochemicals and veterinary 
drugs  Sub Committee under the Food and Agriculture standards Technical Committee, UNBS/TC 2.  DUS 1577: 2014 
iv © UNBS 2014 – All rights reserved  
 Introduction  
Biopesticides are certain types of pesticides derived from such natural material s as animals, plants , fungi  and 
bacteria.  Biopesticides fall into three major classes:  
1. Microbial pesticides consist of a microorganism (e.g., a bacterium, fungus,  nematode,  virus or 
protozoan) as the active ingredient. Microbial pesticides can control many  different kinds of pests, 
although each separate active ingredient is relatively specific for its target pest[s]. For example, there 
are fungi that control certain weeds, and other fungi that kill specific insects.  
2. Plant -Incorporated-Protectants (PIPs) are pesticidal substances that plants produce from genetic 
material that has been added to the plant. For example, scientists can take the gene for the Bt pesticidal protein, and introduce the gene into the plant's own genetic material. Then the plant, inste ad of the Bt bacterium, manufactures the substance that destroys the pest.  
3. Biochemical pesticides are naturally occurring substances that control pests by non-toxic mechanisms. Conventional pesticides, by contrast, are generally synthetic materials that di rectly kill or 
inactivate the pest. Biochemical pesticides include substances,  such as insect sex pheromones, that 
interfere with mating as well as various scented plant extracts that  attract insect pests to traps.  
4. Bio rations (Regulation)  
Biopesticides ar e usually inherently less toxic than conventional pesticides  and generally affect only the target 
pest and closely related organisms, in contrast to broad spectrum, conventional pesticides that may affect organisms as different as birds, insects, and mamma ls. 
Biopesticides often are effective in very small quantities and often decompose quickly, thereby resulting in 
lower exposures and largely avoiding the pollution problems caused by conventional pesticides.  When used 
as a component of Integrated Pest Management (IPM) programs, biopesticides can greatly decrease the use 
of conventional pesticides, while crop yields remain high.  
The objective of this standard is to ensure that biopesticides on the market are appropriately tested through the quality criteria provided while ensuring that farmers obtain only certified products and as well aid the 
industry in the manufacture of quality biopesticides . This standard will also promote the safe use of 
biopesticides  and promote fair trade.  
 DRAFT UGANDA STANDARD  DUS 1577: 2014 
 
© UNBS 2014 – All rights reserved  1 
 Biopesticide — Specificati on 
1 Scope  
This Draft Uganda standard specifies  requirements and methods of sampling and test for biopesticides.  This 
standard does not cover requirements for conventional chemical pesticides  and Plant Incorporated 
Protectants . 
2 Normative references 
The f ollowing referenced documents are indispensable for the application of this document. For dated 
references, only the edition cited applies. For undated references, the latest edition of the referenced 
document (including any amendments) applies.  
Agricultural Chemicals Control Act, 2006 (Act No.1 of 2007)  
Pesticide registration and control regulation, 2010 
3 Terms and definitions  
For the purposes of this standard, the following terms and definitions shall apply .  
3.1  
biopesticide  
form of pesticides derived from such natural materials as animals, pl ants, bacteria and fungi that control pests 
by non-toxic means  
3.2 
active agent/ingredient  
living agent in a biopestcide to which pest control activities are attributed  
4 Requirements 
4.1 General requirements  
The biopesticide shall comply with existing regulatory requirement. The biopesticide shall be fully registered 
as required under the Agricultural Chemicals Control  Act and have undergone a complete efficacy 
assessment and environment impact assessment.  
The base or carrier material used in the manufacture of biopesticide shall be solid or liquid, be free from 
contamination and appropriate for the intended purpose.  
The biopesticide shall be presented in an appropriate form to aid its application. It may be in the form of dry 
powder, granules or liquid.  DUS 1577: 2014  
2 © UNBS 2014 – All rights reserved  
 The biopesticide shall not contain substances that are likely to be detrimental or injurious to vegetation, 
animals, public health or the environment when used according to the directions provided by the 
manufacturer . 
The biopesticide shall not contain substances that would, when applied in amounts commonly used or as 
specified in the directions for use, leave in the tissues of a plant a residue of a poisonous or harmful 
substance.  
4.2 Specific requirements  
Biopestici des shall comply with the specific requirements stipulated in table 1.  
Table 1 —  Specific requirements for biopesticides 
S/No.  Parameter  Limit  Test method  (s) 
i) pH 6.5 – 7.5  
ii) Moisture c ontent, % m/m (For 
biopesticide  produced using solid 
base materi al) 30 – 40  
5 Hygiene  
Biopesticides shall be produced and handled under hygienic conditions and in appropriate premises in order 
to prevent contamination of the product.  
Biopesticides shall comply with the limits for micr o-organisms specified in table 2.  
Table 2  — Microbiological limits for biopesticides  
S/No.  Organism  Limit  Test method  (s) 
i) Viable cell count of the active agent 
at manufacture, cfu/mL or cfu/g, 
min. 
• Metarrhizium anisopliae  
• Trichoderma viride 
• Pseudomonas flourescens  Solid  Liquid   
5 X 107 1 X 108 
ii) Viable cell count of the active agent 
at expiry, cfu/mL  or cfu/g , min . 1 X 105  
iii) Non-target bacteria Contamination , 
cfu/mL  or cfu/g , at 105 dilution,  
max. Nil  
iv) Pathogenic microbes, cfu/mL or 
cfu/g, max.  
i. Salmonella  
ii. Shigella  
iii. Vibrio Nil  
Note: The active agent /ingredient  in biopesticides may be from bacterium, fungus, 
nematode, virus or protozoan DUS 1577: 2014  
© UNBS 2014 – All rights reserved  3 
 6 Weights and measures 
The volume and fill of the biopesticides shall comply with the weights and measures legislation.  
7 Packaging  
The containers, including packaging materials, used to package biopesticide shall be made only of 
substances, which are safe and suitable for their intended uses.  They shall not impart any toxic substance to 
the product  or undesirable odour . 
8 Labelling  
Biopesticides shall be labelled with the following specific requirements and shall be marked legibly and 
indelibly : 
a) name of the product shall be “Biopesticide”;  
b) active ingredient shall appear in close proximity to the name of the product by specifying the genus  
and species of the microorganism;  
c) a brand name may be used to accompany the product name;  
d) microbial density;  
e) name and address of manufacturer, exporter, packer and/or dispatcher ; 
f) type/nature of carrier;  
g) batch or code number;  
h) pests or pest spectrum against  which the biopesticides is effective ; 
i) crops for which it is intended;  
j) storage conditions and instructions;  
k) date of manufacture;  
l) expiry date;  
m) net content;  
n) registration number;  
o) mode of action;  
p) rate and method of application;  
q) directions/instructions for use;  and 
r) safety precautions.  DUS 1577: 2014  
4 © UNBS 2014 – All rights reserved  
 9 Sampling  
9.1 Scale of sampling  
9.1.1 Lot 
All containers in a consignment belonging to the same batch of manufacture shall constitute a lot.  If the 
consignment is declared to consist of different batches of manufacture, containers of the same batch shall be 
grouped together and each group so formed shall constitute a separate lot.  
Sample shall be tested from each lot for ascertaining conformity to the requirements of this standard.  
9.1.2 Sample size 
The number of containers to be selected from a lot for testing for microbiological and other requirements shall 
depend on the size of the lot and shall be in accordance with Table 2.  
Table 2  – Number of containers to be selected for sampling  
No. of containers in the lot  No. of containers to be selected (n)  
Microbiological Other tests  
up to 1300 12 18 
1301 to 3200 18 24 
3201 and above  24 30 
9.1.3 Sampling method 
The containers to be selected for testing shall be chosen at random from the lot by the following procedure.  
Starting fr om any container, count them as 1,2,3…... up to r.  Every rth containers thus counted shall be 
withdrawn, r being the integral part of N/n, where N is the total number of containers in the lot and n is the total 
number of container to be chosen (Table 2 ). 
9.2 Test samples and reference samples 
9.2.1 Samples for microbiological tests 
The sample containers selected for microbiological tests (see col. 2 of Table 2) shall be divided at random into 
three equal sets and labelled with all particulars of sampling.  One of these sets of sample containers shall be 
for the buyer; another for the supplier and the third set is the reference.  
9.2.2 Samples for other tests  
The sample containers selected for ot her tests (see col. 3 of Table 2) shall be divided at random int o three 
equal sets and labelled with all the particulars of the sample.  One of these sets of sample containers shall be 
for the buyer, another for the supplier and third is the reference.  
9.2.3 Reference samples 
Referee samples shall consist of set of sam ple containers for microbiological tests (see 9 .2.1) and a set of 
sample containers for  other tests (see 9.2.2) and shall bear the seals of the buyer and supplier or as agreed to 
between the two.  DUS 1577: 2014  
© UNBS 2014 – All rights reserved  5 
 10 Methods of analysis 
Test of biopesticides shall be done i n accordance with the Uganda Standards  stated in the relevant clauses.  
 DUS 1577: 2014  
6 © UNBS 2014 – All rights reserved  
 Bibliography  
[1]  
[2]  
 DUS 1577: 2014  
© UNBS 2014 – All rights reserved  7 
 Certification marking  
Products that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the f igure below.  
The use of the UNBS Certification Mark is governed by the Standards Act, and the Regulations made thereunder. This mark can be used only by those licensed under the certification mark scheme operated by the Uganda National Bureau of Standards  and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the requirements of that standard under a system of supervision, control and testing in acc ordance with the 
certification mark scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually checked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
 DUS 1577 : 2014    
 
ICS 65.100.01  
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