COMMISSION IMPLEMENTING REGUL ATION (EU) No 848/2014 
of 4 August 2014 
concer ning the author isation of L-valine produced by Cor ynebacter ium glutamicum as a feed addi ­
tive for all animal species and amending Regulation (EC) No 403/2009 as regards the labelling of 
the feed additiv e L-valine 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Having regar d to the Treaty on the Functioning of the European Union, 
Having regar d to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 
on additives for use in animal nutr ition (1), and in particular Article 9(2) and Article 13(2) thereof, 
Whereas: 
(1)  Regulation (EC) No 1831/2003 provides for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting and modifying such author isation. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitt ed for the author isa­
tion of L-valine. That application was accompan ied by the particulars and documents required under Article 7(3) 
of Regulation (EC) No 1831/2003. 
(3)  The application concer ns the author isation of L-valine produced by Corynebacter ium glutamicum (KCCM 80058) as 
a feed additive for all animal species, to be classified in the additive categor y ‘nutr itional additives ’. 
(4)  The European Food Safety Author ity (‘the Auth ority’) concluded in its opinion of 8 Octobe r 2013 (2) that, under 
the proposed conditions of use, the L-valine produced by Corynebacter ium glutamicum (KCCM 80058) does not 
have an adverse effect on animal health, human health or the environment and that it is considered an efficacious 
source of the essential amino acid L-val ine for animal nutr ition. The Author ity does not consider that there is a 
need for specifi c requirements of post-market monitoring. It also verified the repor t on the method of analysis of 
the feed additive in feed submitted by the Reference Laboratory set up by Regulation (EC) No 1831/2003. 
(5)  The assessment of that substance shows that the conditions for author isation, as provided for in Article 5 of 
Regulation (EC) No 1831/2003, are satisfied. According ly, the use of that substance should be author ised as spe­
cified in the Annex to this Regulation. 
(6)  Commission Regulation (EC) No 403/2009 (3) author ised L-valine produced by Escher ichia coli. In order to ensure 
the differentiation of the additives in the final feed, their identif ication number should be labelled on feed mater ­
ials and compo und feed together with their name and added amount. 
(7)  Regulation (EC) No 403/2009 should theref ore be amended according ly. Since the modifi cations to the conditions 
of author isation are not relat ed to safety reasons, it is appropr iate to provide for a transitional period during 
whic h existing stoc ks may be used up. 
(8)  The measures provided for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed, 
HAS ADOPTED THIS REGUL ATION: 
Article 1 
Author isation 
The substance specifi ed in the Annex, belonging to the additive categor y ‘nutr itional additives ’ and to the functional 
group ‘amino acids, their salts and analogues’, is author ised as an additive in animal nutr ition subject to the conditions 
laid down in that Annex. 5.8.2014 L 232/13 Official Jour nal of the European Union EN     
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Jour nal 2013; 11(10):3429. 
(3)Commission Regulation (EC) No 403/2009 of 14 May 2009 concer ning the author isation of a preparation of L-valine as a feed additive 
(OJ L 120, 15.5.2009, p. 3). Article 2 
Amendment to Regulation (EC) No 403/2009 
In the ninth column of the Annex to Regulation (EC) No 403/2009, the following paragraph is added: 
‘Where voluntary declaration of the additive is made on the labelling of feed mater ials and compound feed, the 
following shall be included: 
—  name and identification number of the additive, 
—  added amount of the additive.’ 
Article 3 
Transitional measures 
Feed mat erials and compo und feed as refer red to in Article 2 which are produced and labelled before 25 Febr uary 2015 
in accordance with the rules applicable before 25 August 2014 may continue to be placed on the mark et and used until 
the existing stoc ks are exhaust ed. As regards feed intended for pet animals, the time period for production and labelling 
referred to in the first senten ce shall end 25 August 2016. 
Article 4 
Entr y into force 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the 
European Union. 
This Regulation shall be binding in its entirety and directly applicable in all Member State s. 
Done at Brussels, 4 August 2014. 
For the Commission 
The Presid ent 
José Manuel BARR OSO  5.8.2014 L 232/14 Official Jour nal of the European Union EN     ANNEX 
Identification 
number of 
the additive Name of the 
holder of 
author isation Additive Compo sition, chemical formula, descr iption, 
analytical method. Species or 
categ ory of 
animal Maximum 
age Minimum 
conten t Maximum 
content 
Other provisions End of period 
of 
author isation mg/kg of complet e feed 
with a moisture content of 
12 % 
Categor y of nutr itional additiv es. Functional group: amino acids, their salts and analogues. 
3c370 — L-valine Additiv e composition 
L-valine minimum 98 % (on dry matt er 
basis) 
Char acter isation of the activ e substance 
L-valine ((2S)-2-amino-3-meth ylbuta ­
noic acid) produced by Corynebacter ium 
glutamicum (KCCM 80058) 
Chemical formula: C5H11NO2 
CAS number : 72-18-4 
Analytical method (1) 
For the deter mination of L-valine in the 
feed additive: Food Chemical Codex  
‘L-valine monograph’. 
For the deter mination of valine in 
premixtures, compo und feed and feed 
mater ials: ion exchange chromat o­
graphy coupled with post-column deri­
vatisation and spectrophotometr ic 
detection (HPLC/VIS) — Commission 
Regulation (EC) No 152/2009 (OJ L 54, 
26.2.2009, p.1). All species —   1.  Declarations to be made on 
the labelling of the additive: 
—  moisture content. 
2.Where voluntary declara ­
tion of the additive is made 
on the labelling of feed ma­
terials and compo und feed, 
the following shall be 
included: 
—  name and identification 
number of the additive, 
—  added amount of the 
additive. 25 August 
2024 
(1)  Details of the analytical methods are available at the following address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts   5.8.2014 L 232/15 Official Jour nal of the European Union EN