Amendment to the Ordinance for Enforcement of the Food Sanitation Act and the Specifications and 
Standards for Foods, Food Additives, Etc.  
 
The government of Japan will designate Isobutylamine, Isopropylamine, sec-Butylamine, 
Propylamine, Hexylamine, Pentylamine , and 2 -Methylbutylamine  as authorized food additive s and 
establish the standards for use  and the compositional specifications . 
 
Summary  
The Food Sanitation Act (hereinafter  referred to as “the Act”), in Article 10, prohibits the use 
and the sale of the food additives the Minister of Health, Labour and Welfare (hereinafter referred to 
as “the Minister”) does not designate. In addition, when specifications or standards for foo d additives 
are stipulated in the Specifications and Standards for Foods, Food Additives, Etc. (Ministry of Health 
and Welfare Notification No. 370, 1959) pursuant to Article 11 of the Act, those additives shall not be 
used or sold unless they meet the sta ndards or the specifications . 
 
In response to a request from the Minister, the Committee on Food Additives of the Food Sanitation 
Council under the Pharmaceutical Affairs and Food Sanitation Council (hereinafter referred to as “the 
Committee”) has discusse d the adequacy of the designation of Isobutylamine, Isopropylamine, sec-
Butylamine, Propylamine, Hexylamine, Pentylamine , and 2 -Methylbutylamine  as food additive s. The 
conclusion of the Committee is outlined below.  
 
Outline of conclusion  
The Minister, pursuant to  Article 10 of the Act, should designate Isobutylamine, Isopropylamine, 
sec-Butylamine, Propylamine, Hexylamine, Pentylamine , and 2 -Methylbutylamine  as food additive s 
unlikely to  harm human health  and establish the standards for use and the comp ositional 
specifications pursuant to  Article 11 of the Act (see Attachment  for the details ). 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
  
Attachment  
Isobutylamine  
イソブチルアミン  
 
Standard for use （draft） 
 
Only for flavoring  
 
Compositional specifications （draft） 
 
Substance name  Isobutylamine  
Structural  formula  
 
 
 
Molecular formula   C4H11N 
Molecular weight   73.14  
Chemical name  [CAS number]   2-Methylpropan -1-amine   [78-81-9] 
Content   Isobutylamine contains not less than 95.0% of isobutylamine (C4H11N). 
Description   Isobutylamine occurs as a  colorless to yellow, clear liquid having a characteristic odor.  
Identification   Determine the infrared absorption spectrum of Isobutylamine  as directed in the 
Liquid Film Method under Infrared Spectrophotometry, and c ompare  it with the 
Reference Spectrum. Both spectra exhibit similar intensities of absorption at the 
same wavenumbers.  
Refractive index   nD20: 1.391 –1.400  
Specific gravity   d2525: 0.724 –0.737  
Assay   Proceed as directed in the Peak Area Percentage Method in the Gas Chromatographic Assay 
of Flavoring Agents under the Flavor ing Substance s Tests . Use operating conditions (2)  
except for the column . Use a fused silica tube (0.25 –0.53 mm in internal diameter  and 30 –
60 m in length ) coated with a 0.25 –1 m thick layer of dimethylpolysiloxane  for gas 
chromatography.   
 Reference spectrum  
 
Isobutylamine  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
105 
 
 
 
 
 
75 
 
 
 
 
%T 50 
 
 
 
 
25 
 
 
 
 
0 
4000   
3000   
2000   
1500  
Wavenumber [cm-1]  
1000   
600 
 
 Isopropylamine  
イソプロピルアミン  
 
Standard for use （draft） 
 
Only for flavoring  
 
Compositional specifications （draft） 
 
Substance name  Isopropylamine  
Structural  formula  
 
 
 
Molecular formula   C3H9N 
Molecular weight   59.11  
Chemical name  [CAS number]   Propan -2-amine   [75-31-0] 
Content   Isopropylamine  contains not less than 95.0% of  isopropylamine  (C3H9N). 
Description   Isoprop ylamine occurs as a  colorless to yellow, clear liquid having a characteristic odor.  
Identification   Determine the infrared absorption spectrum of Isopropylamine as directed in the  
Liquid Film Method under Infrared Spectrophotometry, and compare it with the 
Reference Spectrum. Both spectra exhibit similar intensities of absorption at the 
same wavenumbers.  
Refractive index   nD20: 1.367 –1.378  
Specific gravity   d2525: 0.681 –0.693  
Assay   Proceed as directed in the Peak Area Percentage Method in the Gas Chromatographic Assay 
of Flavoring Agents under the Flavoring Substances Tests. Use operating conditions (2) 
except for the column. Use a fused silica tube (0.25 –0.53 mm in internal diamet er and 30 –60 
m in length ) coated with a 0.25 –1 m thick layer of dimethylpolysiloxane  for gas 
chromatography.   
 Reference spectrum  
 
Isopropylamine  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
105 
 
 
 
 
 
75 
 
 
 
 
%T 50 
 
 
 
 
25 
 
 
 
 
0 
4000   
3000   
2000   
1500  
Wavenumber [cm-1]  
1000   
600 
 
 sec-Butylamine  
sec-ブチルアミン  
 
Standard for use （draft） 
 
Only for flavoring  
 
Compositional specifications （draft） 
 
Substance name  sec-Butylamine  
Structural  formula  
 
 
 
Molecular formula   C4H11N 
Molecular weight   73.14  
Chemical name  [CAS number]   Butan -2-amine   [13952 -84-6] 
Content   sec-Butylamine  contains not less than 95.0% of sec-butylamine  (C4H11N). 
Description  sec-Butylamine occurs as a colorless to  yellow, clear liquid having a characteristic odor.  
Identification   Determine the infrared absorption spectrum of sec-Butylamine as directed in th e 
Liquid Film Method under Infrared Spectrophotometry, and compare  it with the 
Reference Spectrum. Both spectra exhibit similar intensities of absorption at the 
same wavenumbers.  
Refractive index   nD20: 1.387 –1.396  
Specific gravity   d2525: 0.715 –0.724  
Assay   Proceed as directed in the Peak Area Percentage Method in the Gas Chromatographic Assay 
of Flavoring Agents under the Flavoring Substances Tests. Use operating conditions (2) 
except for the column. Use a fused silica tube (0.25 –0.53 mm in internal diameter and 30 –60 
m in length ) coated with a 0.25 –1 m thick layer of dimethylpolysiloxane  for gas 
chromatography.   
 Reference spectrum  
 
sec-Butylamine  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
105 
 
 
 
 
 
75 
 
 
 
 
%T 50 
 
 
 
 
25 
 
 
 
 
0 
4000   
3000   
2000   
1500  
Wavenumber [cm-1]  
1000   
600 
 
 Propylamine  
プロピルアミン  
 
Standard for use （draft） 
 
Only for flavoring  
 
Compositional specifications （draft） 
 
Substance name  Propylamine  
Structural  formula  
 
 
 
Molecular formula   C3H9N 
Molecular weight   59.11  
Chemical name  [CAS number]   Propan -1-amine   [107-10-8] 
Content   Propylamine  contains not less than 95.0% of  propylamine  (C3H9N). 
Description  Propylamine occurs as a colorless to  yellow, clear liquid having a characteristic odor.  
Identification   Determine the infrared absorption spectrum of Propylamine  as directed in the Liquid 
Film Method under Infrared Spectrophotometry , and compare it with the Reference 
Spectrum. Both spectra exhibit similar intensities of absorption at the same 
wavenumbers.  
Refractive index   nD20: 1.384 –1.392  
Specific gravity   d2525: 0.710 –0.720  
Assay   Proceed as directed in the Peak Area Percentage Method in the Gas Chromatographic Assay 
of Flavoring Agents under the Flavoring Substances Tests. Use operating conditions (2) except for 
the column. Use a fused silica tube (0.25 –0.53 mm in internal diameter and 30 –60 m in length ) 
coated with a 0.25 –1 m thick layer of dimethylpolysiloxane  for gas chromatography. 
 Reference spectrum  
 
Propylamine  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
105 
 
 
 
 
 
75 
 
 
 
 
%T 50 
 
 
 
 
25 
 
 
 
 
0 
4000   
3000   
2000   
1500  
Wavenumber [cm-1]  
1000   
600 
 
 Hexylamine  
ヘキシルアミン  
Standard for use （draft） 
 
Only for flavoring  
 
Compositional specifications （draft） 
 
Substance name  Hexylamine  
Structural  formula  
 
 
 
Molecular formula   C6H15N 
Molecular weight   101.19  
Chemical name  [CAS number]   Hexan -1-amine   [111-26-2] 
Content   Hexylamine  contains not less than 95.0% of hexylamine  (C6H15N). 
Description  Hexylamine occurs as a colorless to  yellow, clear liquid having a characteristic odor.  
Identification   Determine the infrared absorption spectrum of Hexylamine  as directed in the Liquid 
Film Method under Infrared Spectrophotometry , and compare it with the Reference 
Spectrum. Both spectra exhibit similar intensities of absorption at the same 
wavenumbers.  
Refractive index   nD20:1.415 –1.421  
Specific gravity   d2525: 0.761 –0.767  
Assay   Proceed as directed in the Peak Area Percentage Method in the Gas Chromatographic Assay 
of Flavoring Agents under the Flavoring Substances Tests. Use operating conditions (2) except for 
the column. Use a fused silica tube (0.25 –0.53 mm in internal diameter and 30 –60 m in length ) 
coated with a 0.25 –1 m thick layer of dimethylpolysiloxane  for gas chromatography. 
 Reference spectrum  
 
Hexylamine  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
105 
 
 
 
 
 
75 
 
 
 
 
%T 50 
 
 
 
 
25 
 
 
 
 
0 
4000   
3000   
2000   
1500  
Wavenumber [cm-1]  
1000   
600 
 
 Pentylamine  
ペンチルアミン  
Standard for use （draft） 
 
Only for flavoring  
 
Compositional specifications （draft） 
 
Substance name  Pentylamine  
Structural  formula  
 
 
 
Molecular formula   C5H13N 
Molecular weight   87.16  
Chemical name  [CAS number]   Pentan -1-amine   [110-58-7] 
Content   Pentylamine  contains not less than 95.0% of pentylamine  (C5H13N). 
Description  Pentylamine  occurs as a colorless to  yellow, clear liquid having a characteristic odor.  
Identification   Determine the infrared absorption spectrum of Pentylamine as directed in the Liquid 
Film Method under Infrared Spectrophotometry , and compare it with the Reference 
Spectrum. Both spectra exhibit similar intensities of absorption at the same 
wavenumbers.  
Refractive index   nD20: 1.408 –1.424  
Specific gravity   d2525: 0.750 –0.759  
Assay   Proceed as directed in the Peak Area Percentage Method in the Gas Chromatographic Assay 
of Flavoring Agents under the Flavoring Substances Tests. Use operating conditions (2) 
except for the column. Use a fused silica tube (0.25 –0.53 mm in internal diameter  and 30 –60 
m in length ) coated with a 0.25 –1 m thick layer of dimethylpolysiloxane  for gas 
chromatography.   
 Reference spectrum  
 
Pentylamine  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
105 
 
 
 
 
 
75 
 
 
 
 
%T  50  
 
 
 
 
25 
 
 
 
 
0 
4000   
3000   
2000   
1500  
Wavenumber [cm-1]  
1000   
 
 2-Methylbutylamine  
2-メチルブチルアミン  
 
Standard for use （draft） 
 
Only for flavoring  
 
Compositional specifications （draft） 
 
Substance name  2-Methylbutylamine  
Structural  formula  
 
 
 
Molecular formula   C5H13N 
Molecular weight   87.16  
Chemical name  [CAS number]   2-Methylbutan -1-amine   [96-15-1] 
Content   2-Methylbutylamine contains not less than 95.0% of 2 -methylbutylamine  (C5H13N). 
Description  2-Methylbutylamine occurs as a colorless to  yellow, clear liquid having a characteristic 
odor.  
Identification   Determine the infrared absorption spectrum of 2 -Methylbutylamine as directed in the 
Liquid Film Method under Infrared Spectrophotometry, and compare it with the 
Reference Spectrum. Both spectra exhibit similar intensities of absorption at the 
same wavenumbers.  
Refractive index   nD20: 1.408 –1.423  
Specific gravity   d2525: 0.752 –0.779  
Assay   Proceed as directed in the Peak Area Percentage Method in the Gas Chromatographic Assay  
of Flavoring Agents under the Flavoring Substances Tests. Use operating conditions (2) 
except for the column. Use a fused si lica tube (0.25 –0.53 mm in internal diameter and 30 –60 
m in length ) coated with a 0.25 –1 m thick layer of dimethylpolysiloxane  for gas 
chromatography.   
 Reference spectrum  
 
2-Methylbutylamine  
 
 
105 
 
 
 
 
 
75 
 
 
 
 
%T 50 
 
 
 
 
25 
 
 
 
 
0 
4000   
3000   
2000   
1500  
Wavenumber [cm-1]  
1000   
600