DRAFT UGANDA STANDARD  
  DUS 1921  
First Edition  
2017 -mm-dd 
 
 
 Reference number  
DUS 1921 : 2017  
© UNBS 2017   
  
Body oils — Specification  
 DUS 1921 : 2017  
ii © UNBS 2017  – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2017   
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 DUS 1921 :2017  
© UNBS 2017  – All rights reserved  iii 
 Foreword  
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade , Industry and 
Cooperatives established under Cap 327 , of the Laws of Uganda , as amended .  UNBS is mandated to co -
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT  Agreement  of the World Trade Organisation (WTO).  
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of key stakeholders 
including government, academia , consumer groups, private sector and other interested parties.  
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the  draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
The committee responsible for this document is Technical Committee UNBS /TC 5, Chemicals and 
Environment . 
 DRAFT UGANDA STANDARD  DUS 1921 : 2017  
 
© UNBS  2017 – All rights reserved  1 
 Body oils  — Specification  
1 Scope   
This Daft Uganda Standard specifies  the requirements method of sampling and test for body oils based on 
refined vegetable oils or vegetable oils blends, mineral oils or mixture of the vegetable oils and mineral oil s 
meant for application on the skin   
It does not cover skin creams, lotions , hair oils  and pure essential oils . 
Body o ils for which therapeutic claims are made ar e not covered by this standard.  
2 Normative references   
The following referenced documents referred to in the text in such a way that some or all of their content 
constitutes requirements of this document. For dated references, only the edition cited applies. For undated 
references, the latest edition of the referenced document (including any amendments) applies.  
FDEAS 846, Glossary of terms relating t o the cosmetic industry  
FDEAS 847 - 2: Oils for cosmetic industry — Methods of test — Part 2: Determination of Moisture Content  
FDEAS 847 - 13: Cosmetics — Analytical methods — Part 13: Determination of rancidity  
FDEAS 847 -16, Oils for cosmetic industry — Methods of test — Part 16: Determination of Heavy metal 
Content  
FDEAS 847 -17, Oils for cosmetic industry — Methods of test — Part 17: Physio -chemical tests  
FDEAS 847 -18, Cosmetics — Analytical methods — Part 18: Determination of thermal stability  
FDUS ISO 1 8416,  Cosmetics — Microbiology — Detection of candida albicans   
FDUS ISO 21148 , Cosmetics — Microbiology — General instructions for microbiological examination  
FDUS ISO 22717 , Cosmetics — Microbiology —Detection of Pseudomonas aeruginosa   
FDUS ISO 22718 , Cosmetics — Microbiology — Detection of Staphylococcus aureus  
FDUS ISO 24153 , Random sampling and randomisation procedures  
US EAS 346, Labelling of cosmetics — General requirements   
US EAS 377 (all parts), Cosmetics and cosmetic products  DUS 1921 : 2017  
2 © UNBS 2017  – All rights reserved  
 3 Terms and definit ions  
For the purposes of this document, the terms and definitions given in FDEAS 846  apply.  ISO and IEC 
maintain terminological databases for use in standardization at the following addresses: — ISO Online 
browsing platform: available at http://www.iso.or g/obp .  
4 Types  
There shall be three types of body  oils, namely;  
a) Type 1 — Based on vegetable oils or its blends;  
b) Type 2 — Based on mineral oils; and  
c) Type 3 — Based on a mixture of vegetable oil(s) and mineral oils.  
5 Requirements  
5.1 General requi rements  
5.1.1  Body oils shall be free from any sediment, suspended matter and separated water.  
5.1.2  All ingredients used including dyes, pigment and colours shall conform to US EAS 377(all Parts) 
Cosmetics and cosmetic products.  
5.1.3  The body oils shall be dermatologically safe and shall not cause irritation or harm to the skin when 
used as intended by the manufacturer.  
5.1.4  The body oils shall not have any objectionable odour . 
5.1.5  The product should possess lubricity and spreadability so that on appli cation to areas of the body, it 
should leave a protective film.  
5.2 Specific requirements  
Body oils shall conform to the requirements specified in table 1, when tested according to the method 
indicated therein.  DUS 1921 : 2017  
© UNBS 2017  – All rights reserved  3 
 Table 1 — Specific requirements for body oil s 
Characteristic  Requirement  Test method  
Moisture content, % by mass, max.  0.5 FDEAS 847 - 2 
Acid value, max.  1.0 FDEAS 847 - 4 
Peroxide value, mg/1 000 g, max.  7.5 Annex A 
Rancidity  Shall be free from rancidity  FDEAS 847 - 13 
Total viable count for aero bic mesophyllic 
micro -organisms per g, max. 100 Annex B  
Thermal stability  To pass the test  FDEAS 847 -18 
Lead, mg/kg , max.  20 FDEAS 847 -16  
Arsenic, mg/kg , max.  2 FDEAS 847 -16  
Mercury, mg/kg , max.  2 FDEAS 847 -16  
Pseudomonas aeruginosa  Not detectable in 0.5 g of cosmetic 
product  FDUS ISO 22717  
staphylococcus  aureus  FDUS ISO 22718  
Candida albicans  FDUS ISO 18416  
NOTE  The total amount of heavy metals as lead, mercury and arsenic, in combination, in the finished product shall not exceed 
20 mg/kg  
6 Pack aging 
The product shall be packaged in suitable well -sealed containers that shall protect the contents and shall not 
cause any contamination or react with the product.  
7 Labelling  
In addition to the labelling requirements in US EAS 346, the package sh all be legibly marked with the following 
information:  
a) manufacturer's name and physical address;  
b) product name  as “Body oil s”; 
c) type of body oils  
d) net content of the material when packed;  
e) month and year of manufacture and expiry  
f) storage instructions ; 
g) country o f origin ; and  
h) warning/ precautions.  
8 Sampling  
Random samples of the product for test shall be drawn from the market, factory or elsewhere in accordance 
with FDUS ISO 24153.  DUS 1921 : 2017  
4 © UNBS 2017  – All rights reserved  
 Annex A 
(normative)  
 
Determination of p eroxide value  
A.1 Principle  
The sample is treated in solution with a mixture of acetic acid and a suitable organic solvent and then with a 
solution of potassium iodide. The liberated iodine is titrated with a standard solution of sodium thiosulphate.  
A.2 Reagents  
A.2.1 Glacial acetic acid  
A.2.2 Chloroform  
A.2.3 Potassium iodide solution,  saturated, freshly prepared  
A.2.4 Standard sodium thiosulphate solution, 0.01 N freshly standardized  
A.2.5 Starch indicator solution. Mix 5 g of starch and 0.01 g mercuric iodide with 30 ml of cold water and 
slowl y pour it while stirring into one litre of boiling water. Boil for three minutes. Allow to cool and decant off 
the supernatant clear liquid.  
A.3 Procedure  
A.3.1 Weigh accurately about 5 g of the sample in a 250 -ml glass stoppered conical flask and dissolve  by 
shaking in 30 ml of a mixed solvent containing 3 parts by volume of glacial acetic acid and 2 parts by volume 
of chloroform. Add 0.5 ml of saturated potassium iodide solution, allow the solution to stand for exactly one 
minute with occasional shaking, then add 30 ml of water and titrate with standard sodium thiosulphate 
solution.  
A.3.2 Add the thiosulphate solution until the colour of the titrated solution becomes light yellow. Then add 1 
ml of starch indicator and continue the titration until the disap pearance of the blue colour.  
A.3.3 Carry out a blank determination without using the sample.  
A.4 Calculation  
The peroxide  value, expressed in milligrams per 1 000 grams, shall be calculated as follows:  
Peroxide value = 
MNVV ) (0001 2 1  
Where  
V1 is the volume of standard sodium thiosulphate solution required with the sample;  
V2 is the volume of standard sodium thiosulphate solution required with the blank;  DUS 1921 : 2017  
© UNBS 2017  – All rights reserved  5 
 N is the normality of standard sodium thiosulphate solution; and  
M is the mass, in grams, of t he sample taken for the test.  DUS 1921 : 2017  
6 © UNBS 2017  – All rights reserved  
 Annex  B 
(normative)  
 
Microbiological examination  
B.1 Outline of the method  
The test consists of plating a known dilution of the sample or any digest agar medium (soyabean casein is 
recommended) suitable for the total count of aerobic bacteria and fungi after incubating them for a specified 
period to permit the development of visual colonies.  
Note  Take precaution in ascertaining that only fresh samples, from carefull y sealed containers that had not been 
opened be fore, are used f or this test. T his is very necessary for getting accurate results.  
B.2 Apparatus  
B.2.1 Tubes , of resistant glass, provided with closely fitting metal caps.  
B.2.2 Autoclaves , of sufficie nt size. They shall keep uniform temperature within the chamber up to a nd 
including the sterilizing temperature of 122 ºC. They shall be equipped with, an accurate thermometer, located 
so as to register the minimum temperature within the sterilizing chamber, a pressure gauge and, properly 
adjusted safety valves.  
B.2.3 Petri d ishes , of 10 0 mm diameter and 15 mm depth. The bottom of the dishes shall be free from 
bubbles and scratches and shall be flat so that the medium is of uniform thickness throughout the plate.  
B.2.4 Colony counter , an approved counting aid, su ch as a Quebec  colony counter. If such a counter is not 
available, counting may be done with a lens giving a magnification of one and  a half times of the diameter. In 
order to ensure uniformity of conditions during counting, illumination equivalent to that provided by t he Quebec 
colony counter shall be employed.  
B.3 Media and buffer  
B.3.1 Soyabean casein digest agar media . 
Dissolve 1.5 g of pancreatic digest of casein, 5 g of papic digest of soyabean meal; and 5 g of sodium chloride 
in 100 mL of distilled water contained  in a 2 -litre beaker by heating in a water -bath.  Add 15 g of powdered 
agar and continue boiling until th e agar is completely digested. Adjust the pH to 7.5 with sodium hyd roxide 
solution. Distribute in 20 mL quantities, close the tubes with metal cups and  autoclave at 122 ºC for 20 min. 
After auto -claving, store the tubes in a cool place and use them within 3 weeks.  
B.3.2 Stock solution pH phosphate buffer . 
Dissolve 34 g of monobasic potassium in about 500 mL of water contained  in a 100 mL volumetric flask . 
Adjust the pH to 7.2  0.1 by the addition of so dium hydroxide solution (4 %). Add water to volume and mix. 
Sterilize at 122 ºC for 20 min, store under refrigeratio n. 
B.3.3 Dilute phosphate buffer solution  pH 7.2 . 
Dilute 1 mL of stock solution with disti lled water in the ratio of 1: 800. Fill 50 mL each in coni cal flasks of 100 
mL capacity. Plug the flasks with cotton and sterilize at 122 ºC for 20 min.  DUS 1921 : 2017  
© UNBS 2017  – All rights reserved  7 
 B.4 Sterilization of apparatus  
B.4.1 Tubes  
These shall be sterilized in the autoclave at a temperature of 122 ºC and 1.05 kg/cm pressure for 20 min or in 
the hot air oven at 180 C for one hour.  
B.4.2 Petri dishes  
These shall be packed in drums and autoclaved at 122 ºC and 1.85 kg/cm pressure for 20 min or individually 
wrapped in kraft paper and sterilized in hot oven at 160 ºC for 1 h.  
B.4.3 Pipettes  
These shall be placed in pipette cones (copper, stainless steel or aluminium) after plugging the broader and 
with, cotton and sterilized in the autoclave at 122 ºC and 1.05 kg/cm pressure for 20 min or at 160  ºC for 1 h in 
hot air oven.  
B.5 Procedure  
B.5.1 Melt a sufficient number of soyabean casein, digest agar medium tubes in hot water -bath and transfer 
while hot into a constant temperat ure water -bath maintained at 48  2 ºC. 
B.5.2 Weigh and transfer aseptic ally 1 g of the sample to a conical flask containing sterile 50 mL, or any 
suitable dilution factors, of dilute phosphate buffer at pH 7.2.  Shake well.  Pipette out in 1 mL portions into 
three sterile petri dishes.  Pour melted and cooled (at 45 ºC) soyab ean casein digest agar medium over it, and 
rotate the plates to mix thoroughly.  Incubate the plates at 32 ºC for 72 h in an inverted position.  
B.6 Expression of results  
Get the average number of colonies on soyabean casein digest agar medium plates determ ine the number of 
micro -organisms per gram of the sample.  If no colony is recovered from any of the plates it can be stated as 
less than 50 micro -organisms per gram.  DUS 1921 : 2017  
8 © UNBS 2017  – All rights reserved  
 Bibliography  
[1] 76/768/EEC, The European Economic Community Cosmetics Directive  
[2] ISO 9001:2015, Quality management systems — Requirements  
[3] KS 1766:2006, Specification for body oils  
[4] US EAS 339:2013, Hair creams, lotions and gels — Specification  
 DUS 1921 : 2017  
© UNBS  2017 – All rights reserved  9 
 Certification marking  
Products that conform to Uganda standards may be marked with Ugand a National Bureau of Standards 
(UNBS) Certification Mark shown in the figure below.  
The use of the UNBS Certification Mark is governed by the Standards Act, and the Regulations made 
thereunder. This mark can be used only by those licensed under the certif ication mark scheme operated by 
the Uganda National Bureau of Standards and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing in accordance with the 
certification mark scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually checked by UNBS for conformity to that standard.  
Further par ticulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
 DUS 1921 : 2017    
 
ICS 71.100.70 
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© UNBS 2017 – All rights reserved