UGANDA ST ANDARD  
  DUS 162 4-1 
First Edition  
2015 -mm-dd 
 
 
 Reference number  
DUS 162 4-1: 2015  
© UNBS 2015   
  
Hair shampoo—  Part 1:  synthetic detergent-based — Specification  
 DUS 1624-1: 2015 
ii © UNBS 2015  – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2015   
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS. 
Requests for permission to reproduce this document should be addressed to 
The Executive Director 
Uganda National Bureau of Standards 
P.O. Box 6329 
Kampala 
Uganda 
Tel: 256 414 505 995 
Fax: 256 414 286 123 
E-mail: info@unbs.co.ug  
Web: www.unbs.go.ug  DUS 1624-1:2015 
© UNBS 2015  – All rights reserved  iii 
 Contents Page 
Foreword .............................................................................................................................................................  v 
1 Scope  ................................ ................................ ................................ ................................ ...................... 1 
3 Normative references  ................................ ................................ ............................................................  1 
4 Classification  ................................................................ ................................ ................................ ......... 1 
5 General requirements  ................................ ................................ ...........................................................  1 
6 Ingredients  ................................................................ ................................ ................................ ............. 2 
7 Packaging  ................................ ................................ ................................ ................................ ............... 3 
8 Marking an d labelling  ................................ ................................ ............................................................  3 
9 Sampling  ................................ ................................ ................................ ................................ ................ 3 
Random samples of the product shall be drawn for test in accordance with ISO 24153 from the 
market, factory or elsewhere ................................................................................................................ 3  
Annex A (informative)  List of ingredients conventionally used in formulation of synthetic 
detergent based shampoo .................................................................................................................... 4  
A.1 Detergents  ................................ ................................ ................................ ................................ .............. 4 
A.2 Foam stabilizers  ................................ ................................................................ ................................ .... 4 
A.3 Solubilizing agents  ................................ ................................ ................................................................  4 
A.4 Preservatives  ................................................................ ................................ ................................ ......... 5 
A.5 Opacifying agents  ................................ ................................................................ ................................ . 5 
A.6 Inorganic salts  ................................................................ ................................ ................................ ....... 5 
A.7 Emollients  ................................ ................................ ................................ ................................ .............. 5 
A.8 Thickening agents  ................................ ................................................................ ................................ . 5 
A.9 Other groups of ingredients  ................................ ................................ ................................ ................. 6 
Annex B (normative)  Determination of active detergent content ................................................................ . 7 
B.1 Outline of the method  ................................ ................................ ...........................................................  7 
B.1.1  Reagents  ................................ ................................ ................................ ................................ ................ 7 
B.1.2  Procedure  ................................ ................................ ................................ ................................ ............... 7 
B.2 Alternative method for determination of active detergent content  ................................ .................. 8 
B.2.1  Field of application  ................................ ................................ ................................................................  8 
B.2.2  Principle  ................................ ................................ ................................ ................................ ................. 9 
B.2.3  Reagent  ................................ ................................ ................................ ................................ .................. 9 
B.2.4  Apparatus  ................................ ................................ ................................ ................................ ............. 10 
B.2.5  Procedure  ................................ ................................ ................................ ................................ ............. 11 
B.2.6  Expression of results  ................................ ................................ ..........................................................  11 
Annex C (normative)  Determination of matter insoluble in alcohol. ..........................................................  13 
C.1 General  ................................ ................................ ................................ ................................ ................. 13 
C.2 Method  ................................ ................................ ................................ ................................ .................. 13 
C.3 Reagents  ................................ ................................ ................................ ................................ .............. 13 
C.4 Procedure  ................................ ................................ ................................ ................................ ............. 13 
C.5 Calculation  ................................................................ ................................ ................................ ........... 13 
Annex D (normative)  Determination of lather volume. ................................................................................ 15 
D.1 General  ................................ ................................ ................................ ................................ ................. 15 
D.2 Outline of the method  ................................ ................................ .........................................................  15 
D.3 Reagents  ................................ ................................ ................................ ................................ .............. 15 
D.4 Apparatus  ................................ ................................ ................................ ................................ ............. 15 
D.5 Preparation of standard hard water  ................................................................ ................................ ... 15 
D.6 Procedure  ................................ ................................ ................................ ................................ ............. 15 DUS 1624-1: 2015 
iv © UNBS 2015  – All rights reserved  
 D.7 Calculation  ................................ ................................ ................................ ...........................................  16 
Annex E (normative)  Determination of pH ................................................................................................... 17 
E.1 Apparatus  ................................ ................................ ................................ ............................................  17 
E.1.1  pH meter equipped with glass electrode.  ................................ ................................ .........................  17 
E.2 Procedure  ................................ ................................ ................................ ............................................  17 
Annex F (normative)  Antibacterial test ......................................................................................................... 18 
F.1 Procedure  ................................ ................................ ................................ ............................................  18 
F.2 Antifungal test  ................................ ................................ ................................ ................................ ..... 18 
F.3 Results  ................................ ................................ ................................ .................................................  18 
 DUS 1624-1:2015 
© UNBS 2015  – All rights reserved  v 
 Foreword 
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Tourism, Trade and 
Industry established under Cap 327, of the Laws of Uganda.  UNBS is mandated to co-ordinate the 
elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT/SPS Agreements of the World Trade Organisation (WTO). 
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of representatives of 
consumers, traders, academicians, manufacturers, government and other stakeholders. 
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
Committee membership DUS 1624-1: 2015 
vi © UNBS 2015  – All rights reserved  
  
 DRAFT EAST AFRICAN STANDARD  DUS 1624 -1:201 
5 
i  
 Hair shampoo  —Part 2: synthetic detergent-based — Specification  
1 Scope 
This Draft Uganda Standard prescribes the requirements, methods of test and sampling for hair 
shampoo, synthetic detergent-based. This standard also includes shampoos with positive dermatological effect 
on the skin and neutralizing shampoos 
. 
3 Normative references 
The following referenced documents are indispensable for the application of this document. For dated 
references, only the edition cited applies. For undated references, the latest edition of the referenced 
document (including any amendments) applies. 
EAS 346, Labelling of cosmetics — General requirements 
EAS 377- 1, Cosmetics and cosmetic products  Part 1: List of substances prohibited in cosmetic products 
EAS 377- 2, Cosmetics and cosmetic products  Part 2: List of substances which cosmetic products must not 
contain except subject to the restrictions laid down 
EAS 377- 3, Cosmetics and cosmetic products  Part 3: List of colorants allowed in cosmetic products 
EAS 377- 4, Cosmetics and cosmetic products  Part 4: List of preservatives allowed in cosmetic products 
EAS 377- 5, Cosmetics and cosmetic products  Part 5: Use of UV filters in cosmetic products 
ISO 24153, Random sampling and randomization procedures 
4 Classification 
Synthetic detergent shampoo shall be classified into four types:  
a) General purpose shampoo — This shall be for "Dry", "Oily" or "Normal" hair, and shall be indicated so. 
b) Treatment shampoo 
c) Baby shampoo 
d) Neutralizing shampoo 
5 General requirements  
5.1 The shampoo shall be in the form of a liquid, emulsion or paste. It may be coloured and perfumed.  
ii © UNBS 2015  – All rights reserved  
 5.2 The clear and /or transparent liquid shampoo, when examined visually shall be free from any sediment. 
a) If in the form of an emulsion, it shall be homogenous and there shall be no visible signs of the 
emulsion having separated. 
b) Shampoo in the form of a paste shall be free from any agglomerated particles. 
5.3 The shampoo shall not be harmful to the user, and shall have no undesirable effect on the natural 
colour of the hair.  (This does not include hair already treated with hair dyes). 
5.4 The shampoo shall impart all the effects claimed (e.g. dandruff control). 
5.5 The "General purpose shampoo" and the "Treatment shampoo" shall comply with the requirements 
given in Table 1 
5.6 The "Baby shampoo" shall comply with the requirements given in Table 2. 
5.7 The "Neutralizing shampoo" shall comply with the requirements given in Table 3. 
6 Ingredients 
6.1 All ingredients used including dyes, pigments and colours shall conform to EAS 377 (all parts). 
6.2 Any product containing ingredients for which medicinal claims are made shall be registered with the 
relevant regulatory agency. 
6.3 All essential oils/herbs used shall conform to the approved standards. 
6.4 All products containing herbal extracts known to have antibacterial activity e.g. neem oil/extract and 
aloe oil/extract shall pass the test for antibacterial activity outlined in Annex F. 
6.5 If neem oil is used as part of the formulation, the amount shall be 5 - 10 % m/m. 
6.6  A  list  of  ingredients  conventionally used in the formulation of shampoos is given for guidance in 
Annex A. 
6.7  All active ingredients including detergents, anti-bacterial or anti-dandruff agents shall be named. Any 
further information concerning the active ingredients shall be supplied by the manufacturer on request. 
6.8  It shall be the responsibility of the manufacturer to ensure the dermatological safety of their 
formulations.  
6.9 For baby shampoos, the active detergent, perfume and all other ingredients shall be of such nature and in 
such amounts as to leave the final baby product mild in nature. This is due to the sensitive nature of baby 
skin. 
 
Table 1 — Requirements for general purpose and treatment shampoo, synthetic detergent based 
Sl no.  Characteristic  Requirement  Test method  
i) Active detergent matter content, % by mass, min.  6 Annex B  
ii) Matter insoluble in alcohol, % by mass, max.  2.0 Annex C  
iii) Lather volume for 1 % solution, mL, min.   100  Annex D   
© UNBS 2015  – All rights reserved  iii 
 iv) pH at 27 ±2 0C 5.0 to 9.0  Annex E  
v) 
 Antibacterial activity, min.  0.2 mm  Annex F  
Table 2 — Requirements for baby shampoo, synthetic detergent based 
 
Sl no.  Characteristic  Requirement  Test method  
(1) (2) (3) (4) 
i) Active detergent matter content, % by mass, 
min. 5.0 Annex B  
ii) Matter insoluble in alcohol, % by mass, max.  2.0 Annex C  
iii) pH at 27 ±2 0C 5.0 to 7.0  Annex E 
iv) Lather volume for one % solution, mL, min  100 Annex D 
v) Antibacterial activity  0.2 mm  Annex F  
Table 3  — Requirements for neutralizing shampoo, synthetic detergent-based 
Sl No.  Characteristic   
Requirement   
Test Method  
(1) (2) (3) (4) 
i) Active detergent matter content, percent by 
mass, min.  10.0 Annex B  
ii) Matter insoluble in alcohol, per cent by mass, 
max.  2.0 Annex C  
iii) pH at 27 ±2 0C 4.0 to 5.0  Annex E 
iv) Lather volume for 1 % solution, mL, min.  100 Annex D 
v) Antibacterial activity  0.2 mm  Annex F  
7 Packaging  
The synthetic detergent-based shampoo shall be packed in suitable containers. 
8 Marking and labelling 
In addition to the labeling requirements outlined in EAS 346, the package shall be legibly marked with the 
following information in or any other language as agreed between the manufacturer and the supplier. 
a) type of shampoo; 
9 Sampling 
Random samples of the product shall be drawn for test in accordance with ISO 24153 from the 
market, factory or elsewhere   
iv © UNBS 2015  – All rights reserved  
  
Annex A 
(informative ) 
 
 
List of ingredients conventionally used in formulation of synthetic detergent based 
shampoo 
A.1 Detergents 
a) Sodium or potassium or ethanolamine salts of lauryl sulphonic acid 
b) Lauryl ether sulphates 
c) Sulphated monoglycetides 
d) Sodium alkyl sulpho-acetate 
e) Alkyl benzene polyoxyethyl sulphonates 
f) Sodium n-lauryl sarcosinate  
g) Sodium alpha olefin sulthonates  
h) Other synthetic detergents 
A.2 Foam stabilizers 
a) Ethanolamides or isopropanolamides of fatty acids 
b) Amine oxides 
c) Cocobetaines  
d) Cocomidopropyl betaines 
A.3 Solubilizing agents  
a) Urea  
b) Aliphatic alcohols 
c) Sodium toluene sulphonate 
d) Sodium xylene sulphonate  
© UNBS 2015  – All rights reserved  v 
 A.4 Preservatives 
a) Alcohols  
b) Formaldehyde 
c) Esters of p-hydroxybensoic acid 
d) Sorbic acid 
e) Imidozolidinyl urea 
A.5 Opacifying agents 
a) Higher fatty alcohols  
b) Ethylene / propylene glycol stearates 
c) Mono and di-stearates of glycerol  
d) Zinc, calcium and magnesium salts of fatty acids 
e) PEG - distearates 6000 
f) Polyacrylates 
A.6 Inorganic salts 
a) Sodium chloride  
b) Sodium sulphate 
c) Sodium phosphate  
d) Ammonium sulphate  
e) Ammonium phosphate  
f) Ammonium chloride 
A.7 Emollients  
a) Lanolin and its derivatives  
A.8 Thickening agents  
a) Sodium carboxymethyl cellulose  
b) Methyl cellulose  
c) Methyl isopropyl cellulose 
d) Guar gum  
vi © UNBS 2015  – All rights reserved  
 A.9 Other groups of ingredients   
a) Perfumes  
b) cosmetic courants  
c) Conditioning agents  
d) Antidandruff  agents  
e) Anti-bacterial agents  
f) Quaternary compounds  
g) Vitamins and proteins 
h) Vegetable oils and mineral oils. 
i) Silicones  
j) Sunscreens, etc.  
© UNBS 2015  – All rights reserved  vii 
 Annex B 
(normative) 
 
Determination of active detergent content 
B.1 Outline of the method   
When equivalent amounts of cationic and anionic detergents are present in a two-phase mixture of water and 
chloroform, methylene blue will colour the two phases to the same degree.  Sodium alkyl benzene sulphonate 
and sodium lauryl sulphate or any other detergent can be titrated with a standard solution of  cetyl trimethyl 
ammonium bromide. 
B.1.1 Reagents 
B.1.1.1 Cationic solution (Solution A)  
Weigh 1.5 " 0.001 g of cetyl trimethyl ammonium bromide into a 250 mL beaker.  Add  100 mL of distilled water 
and stir until dissolved.  Transfer quantitatively to a 1 litre volumetric flask and make to volume.  Mix thoroughly 
and standardize against solution B.  (See  B.1.2.2). 
B.1.1.2 Anionic solution (Solution B)  
Weigh accurately such amount of standard alkyl sulphate of known combined SO 3 or active content so as to give 
exactly 0.320 g of combined SO 3 into a 250 mL beaker.  Dissolve in 100 mL to 200 mL of warm water.  Transfer 
quantitatively to 1-litre volumetric flask and make to volume with water at room temperature. Mix thoroughly.  This 
is the primary standard against which solution A, is standardized.  Solution B is 0.004 N. 
B.1.1.3 Methylene blue indicator  
Dissolve 0.1 g of methylene blue in 100 mL of water.  Transfer 30 mL of this solution to a 1 litre flask.  Add 500 mL 
of water, 6.8 mL of concentrated sulphuric acid, 50 g of sodium phosphate (NaH 2PO 4H2O) and shake until 
solution is complete.  Dilute to the mark. 
B.1.1.4 Chloroform   
Analytical reagent grade  
B.1.2 Procedure 
B.1.2.1  Weigh accurately a sample of sufficient size to give approximately 0.320 g of combined SO 3 into a 
250 mL beaker.  Sample size is crucial (see Note).  Use 700 mL to 800 mL of warm water to transfer 
quantitatively to a 1-litre volumetric flask.  Warm on steam bath and shake gently until the sample is dissolved 
and solution is clear.  Cool, dilute to the mark and mix thoroughly. 
NOTE The titration value V  should be as near as to 10 mL as possible, say between 8 mL and 12 mL but never 
outside 5 mL and 15 mL. 
B.1.2.2  Pipette 10.0 mL of the sample solution into a 100 mL glass stoppered cylinder (25 x 300 mm).  
Add 25.0 " 0.5 mL of methylene blue solution and 10 " 0.5 ml of chloroform (see Note).  Titrate with solution A 
to the correct end point, shaking the cylinder carefully after such addition to avoid emulsion and maintaining 
temperature within prescribed limits of  20 C  to  30 C by immersion in water bath, if necessary.  As the end 
point is approached, the rate of transfer of colour increases and solution A shall be added dropwise with  
viii © UNBS 2015  – All rights reserved  
 vigorous shaking after each addition.  If the approximate titration is known, before shaking since this avoids 
emulsion formation. 
Application of vacuum to the titration cylinder may help to break some emulsions, if formed. The end point is 
reached when both layers have same colour intensity.  The end point is very sharp and 0.05 mL will cause a 
distinct change in colour distribution at or near the equivalence point. 
NOTE  The titration value V should be as near to 10 mL as possible, say between 8 mL and 12 mL but never outside 5 mL 
and 15 mL. 
B.1.2.3 Pipette 10.0 mL of the sample solution into a 100 mL glass stoppered cylinder (25 x 300 mm).  
Add 25.0 " 0.5 mL of chloroform (see Note).  Titrate with solution A to the correct end point, shaking the 
cylinder carefully after such addition to avoid emulsion and maintaining temperature within prescribed limits of 
20EC to 30EC by immersion in water bath if necessary.  As the end point solution A shall be added dropwise 
with vigorous shaking after each addition.  If the approximate titration is known, 80 % of the required titrating 
solution should be added before shaking since this avoids emulsion formation. 
Application of vacuum to the titration cylinder may help to break some emulsions, if formed.  The end point is 
reached when both layers have same colour intensity.  The end point is very sharp and 0.05 mL will cause a 
distinct change in colour distribution at or near equivalence point. 
NOTE   The volume of methylene blue solution and chloroform may be changed if found advantageous provided the same 
volumes are used in standardizing solutions A and B. 
B.1.2.4 Calculation 
i) % combined  SO 3   =  
MNV 8.0 x   x  
where,   
 V=volume, in mL of solution A used in the titration; 
 N=vormality of solution A; and 
 M=mass, in g of the sample in the aliquot. 
ii) Per cent active detergent content = per cent combined SO 3 x Mol. weight of active detergent. 
NOTE  The molecular weight of active detergent shall be supplied by the manufacturer on request. 
B.2 Alternative method for determination of active detergent content 
(To be used only if the first method (Clause B.1) fails to work on the product). 
B.2.1 Field of application 
This method is applicable to the analysis of alkylbenzene sulphonates, alkyl sulphonate, sulphates and 
hydroxy-sulphates, alkylphenol and fatty alcohol ethoxysulphates  and dialkyl sulphosuccinates and to the 
determination of active materials containing one hydrophilic group per molecule.  
© UNBS 2015  – All rights reserved  ix 
 B.2.2 Principle 
Determination of anionic-active matter in a medium consisting of an aqueous and chloroform phase, by 
volumetric titration with a standard cationic-active solution (benzethonium chloride), in the presence of an 
indicator which consists of a mixture of a cationic dye (dimidium bromide) and an anionic dye (acid blue 1). 
B.2.3 Reagent 
B.2.3.1  The water used shall be of distilled quality. 
B.2.3.2  Chloroform, (sp. gravity = 1.48 g/m, distilling between 59.5E and 61.5E). 
B.2.3.3  Sulphuric acid,  2.5 M solution. 
B.2.3.4  Sulphuric acid,  0.5 M solution. 
B.2.3.5  Sodium hydroxide,  1.0 M standard volumetric solution. 
B.2.3.6  Sodium lauryl sulphate (sodium dodecyl sulphate) (CH 3(CH 2) (11 OSO 3Na), 0.004 M standard 
volumetric solution.  
Check the purity of the sodium lauryl sulphate and simultaneously prepare the standard solution. 
B.2.3.6.1  Determination of purity of sodium lauryl sulphate  —  Weigh to the nearest 1 mg, 5 " 0.2 g  of the 
product into a 250 mL round bottom flask with ground glass neck.  Add exactly 25 mL of the sulphuric acid 
solution (B.2.3.4) and reflux into a water condenser. 
During the first 5 min to 10 min, the solution will thicken and tend to foam strongly; control this by removing the 
source of heat and swirling the contents of the flask. 
In order to avoid excessive foaming, instead of refluxing the solution may be left on a boiling water bath for 60 
min. 
After a further 10 min the solution clarifies and foaming ceases .  Reflux for further 90 min. Remove the source 
of heat, cool the flask and carefully rinse the condenser with 30 mL of ethanol followed by water. 
Add a few drops of the phenolphthalein solution (B.2.3.8) and titrate the solution with the sodium hydroxide 
solution (B.2.3.5). 
Carry out  a blank test by titrating 25 mL of the sulphuric acid solution (B.2.3.4) with the sodium hydroxide 
solution (B.2.3.5). 
The purity of the sodium lauryl sulphate, expressed as a percentage,  
 
1O O 1   ) ( 28.84
MM VV   
where, 
V0 =Volume, in millilitres, of sodium hydroxide solution used for the blanktest; 
V1=Volume, in millilitres, of sodium hydroxide solution used for the sample; 
m1=mass, in grams, of the sodium lauryl sulphate to be checked; and  
M0=exact molarity of the sodium hydroxide solution.  
x © UNBS 2015  – All rights reserved  
 B.2.3. 6.2 Weigh 0.004 M sodium lauryl sulphate standard volumetric solution.  Weigh, to the nearest 1 mg 
between 1.14 g and 1.16 g of sodium lauryl sulphate and dissolve in 200 mL of water.  Transfer to a ground 
glass stoppered 1 litre one-mark volumetric flask and dilute to the mark with  water. 
Calculate the molarity, M1, of the solution by means of the solution by means of the formula: 
 
 
100 x 288.4(%)  x   2
1purity m   M  
where,  
 m 2 = mass  in grams of sodium lauryl sulphate. 
B.2.3.7  Benzethonium chloride 0.004 M standard volumetric solution  
Weigh, to the nearest 1 mg, between 1.75 g and 1.85 g benzethonium chloride and dissolve in water. 
Transfer to a ground  glass-stoppered  1 litre one-mark volumetric flask and dilute to the mark with water. 
NOTE  In order to prepare a 0.004 M solution, dry the benzethonium chloride at 105EC, weigh 1.792 g, to the nearest 1 
mg, dissolve in water and dilute to 1 litre. 
B.2.3.8  Phenolphthalein, ethanolic  solution containing 10 g/L.  Dissolve 1 g of phenolphthalein in 100 mL 
of 95 per cent (v/v) ethanol. 
B.2.3.9 Mixed indicator 
B.2.3.9.1 Stock solution  
Weigh to the nearest 1 mg 0.5 " 0.005 g dimidium bromide into a 50 mL beaker, and 0.025 " 0.005 g of acid 
blue 1 into a second 50 mL beaker.  
Add between 20 mL and 30 mL of hot 10 %. (v/v) ethanol to each beaker.  Stir until dissolved and transfer the 
solutions to a 250-mL  one mark volumetric flask.  Rinse the beakers into the volumetric flask with ethanol and 
dilute to the mark with 10 % (v/v) ethanol. 
B.2.3.9.2 Mixed acid indicator solution  
Take 20 mL of the stock solution prepared above, put it in a 500 mL one-mark volumetric flask.  Add 200 mL of 
water, and 20 mL of 2.5 M sulphuric acid (B.2.3.2) mix and dilute to the mark with water.  Store out of direct 
sunlight. 
B.2.4 Apparatus     
Ordinary Laboratory apparatus, and  
a) Bottles, 200 mL, glass stoppered, or measuring cylinders, flask stoppered. 
b) Burettes, 25 mL and 50 mL. 
c) One-mark volumetric flask, 1-litre capacity glass stoppered. 
d) One-mark pipette, 25 mL.  
© UNBS 2015  – All rights reserved  xi 
 B.2.5 Procedure 
B.2.5.1 Standardization of benzethonium chloride solution    
By means of the pipette transfer 25 mL of the 0.004 M sodium lauryl sulphate solution to a bottle or measuring 
cylinder, add 10 mL of water, 15 mL of the chloroform and 10 mL of the mixed indicator solution. 
Titrate with the 0.004 M benzethonium chloride solution.  Stopper the bottle or measuring cylinder after each 
addition and shake well.  The lower layer will be coloured pink.  Continue the titration with repeated vigorous 
shaking.  As the end point approaches, the emulsions formed during shaking tend to break easily Continue the 
titration drop by drop.  Shaking after each addition of titrant, until the end point is reached.  This is at the moment 
when the pink colour is completely discharged from the chloroform layer, which becomes a faint greyish blue. 
The molarity, M, of the benzethonium chloride solution is given by the formula: 
 
2125 x   VM   M  
where,   
M1=molarity of the sodium lauryl sulphate solution; and 
V2=Volume, in mollolitres, of benzethonium chloride added. 
B.2.5.2 Determination  
Weigh to the nearest 1 mg a sample of 30 g, dissolve the test portion in water.  Add a few drops of the 
phenolphthalein solution and neutralize to a faint pink colour with the sodium hydroxide solution or sulphuric acid 
solution as required. 
 Transfer to a 1 litre one-mark volumetric flask and dilute to the mark with water.  Mix thoroughly and, by means of 
the pipette transfer 25 mL of this solution to a bottle or measuring cylinder, add 10 mL of water, and add 10 mL of 
water, and 15 mL of chloroform.  Titrate with the benzethonium chloride solution as described in B.2.5.1. 
B.2.6 Expression of results   
The content as a percentage by mass, of anionic-active matter   
 
oo 3
 x 000  1 x 25100x  x 000  1  x  x  
MM M V  
 =  4 V3M   
The amount of active matter, expressed in milliequivalents per gram, 
 
o1 3 x  x 40
MM V  
where,  
M0=mass, in grams, of the test portion; 
M=relative molar mass of anionic-active matter; 
M1=molarity of the benzethonium chloride solution;  
xii © UNBS 2015  – All rights reserved  
 V3=Volume, in millilitres, of benzethonium chloride solution used for the titration of a 25 ml aliquot of 
anionic-active matter solution.  
© UNBS 2015  – All rights reserved  xiii 
 Annex C 
(normative) 
 
Determination of matter insoluble in alcohol. 
C.1 General  
This method may be used for the approximate determination of these constituents.   As these salts are not 
completely insoluble in alcohol, separate portions of soap should be used for accurate determination, 
employing specific methods. 
C.2 Method  
It consists in digesting the material in alcohol and filtering off the residue, which is dried and weighed. 
C.3 Reagents  
C.3.1  Phenolphthalein indicator, dissolve 1 g in 100 mL of 95 % rectified spirit. 
C.3.2  Ethyl alcohol or rectified spirit, freshly boiled and neutral to phenolphthalein. 
C.4 Procedure  
C.4.1  Weigh accurately 2 g to 10 g of the sample and digest with 200 mL of freshly boiled ethyl alcohol in a 
covered vessel on a steam bath until the soap is dissolved.  Filter into a filter flask through a tared, dried and 
counterpoised filter paper till neutral to phenolphthalein, or through a tared and dried Gooch or sintered glass 
crucible with suction, protecting the solution from carbon dioxide and other acid fumes during the operation by 
covering with a watch glass.  The filter paper and Gooch crucible shall be prepared as per the method given 
under C4.2.   
C.4.2 Wash it several times with hot ethyl alcohol to remove all the alcohol solubles.  Dry the filter paper or 
the crucible with residue at 100 ± 2 C for 3 hours and cool.  Weigh the total matter insoluble in alcohol. 
C.4.3  Place filter paper in a weighing bottle and dry in an air oven at 105 ± 2 C, with cover removed.  
Remove from the oven, replace cover, cool to room temperature in desiccator and weigh.  Prepare the Gooch 
crucible with a pad of asbestos fibre.  Wash the pad with water, alcohol and ether and then dry to constant 
mass at 105 ± 2 C, cool to room temperature in a desiccator and weigh. 
C.5 Calculation  
Matter insoluble in alcohol, 
 
% by mass =
21100
MM   
xiv © UNBS 2015  – All rights reserved  
 where, 
M1 = mass, in g of matter insoluble alcohol; 
M2 = mass, in g of the material taken for the test.  
© UNBS 2015  – All rights reserved  xv 
 Annex D 
(normative) 
 
Determination of lather volume. 
D.1 General  
Strict attention shall be paid to all details of the procedure in order to ensure concordant results.  Particular 
care should be taken to invert the cylinder exactly as described. 
D.2 Outline of the method  
A suspension of the material in standard hard water is taken in a graduated cylinder and given 12 inversions 
under prescribed conditions.  The volume of the foam formed is observed after keeping the cylinder for 
minutes. 
D.3 Reagents  
D.3.1  Calcium chloride (CaCl 22H20, chemically pure. 
D.3.2  Magnesium sulphate (MgSO 4.7H 20), chemically pure. 
D.3.3  Distilled water. 
D.4 Apparatus  
a) Graduated cylinder —  Glass stoppered with graduations from 0 ml to 25 mL, with 2 mL divisions. 
Overall height about 35 cm and the height of the graduated portion about 20 cm. 
b) A 100 mL glass beaker. 
c) Thermometer of range 0 C to 110 C. 
D.5 Preparation of standard hard water  
Dissolve 0.220 g of chemically pure calcium chloride , and 0.246 g of chemically pure magnesium sulphate in 
distilled water.  Dilute to 5 litres with distilled water. 
NOTE  This standard hard water has a hardness of approximately 50 pm calculated as calcium carbonate. 
D.6 Procedure  
Weigh 2 g of the shampoo accurately in a 100 mL glass beaker.  Add 10 mL of the standard hard water.  
Cover the beaker with a watch glass and allow to  stand for 30 minutes.  This operation is carried out to 
disperse the shampoo.  Stir the contents of the beaker with a glass rod and transfer the slurry to a 250 mL 
graduated cylinder ensuring that not more than 2 mL foam is produced.  Repeat the transfer of the residue left  
xvi © UNBS 2015  – All rights reserved  
 in the beaker with further portions of 20 mL of standard hard water ensuring that all the matter in the beaker is 
transferred to the cylinder. 
Bring the contents of the cylinder to 30 C.  Stir the contents of the cylinder with a glass rod or thermometer to 
ensure a uniform suspension. 
As soon as the temperature of the contents of the cylinder reach 30 C, stopper the cylinder and give it 12 
complete inversions, each inversion comprising movements in a vertical plane, upside down and vice versa. 
After the 12 inversions, let the cylinder stand for 15 minutes.   Take the following readings as shown in Figure 
D.1. 
a) Foam plus water (V 1mL) 
b) Water  only (V 2mL) 
 
                   Figure D.1 — Measurement of foam 
D.7 Calculation  
Lather volume = V1 - V2 
where, 
V1 = volume, in mL of foam and water; 
V2 = volume, in mL of water only.  
© UNBS 2015  – All rights reserved  xvii 
 Annex E 
(normative) 
 
Determination of pH 
E.1 Apparatus 
E.1.1 pH meter equipped with glass electrode. 
E.2 Procedure 
Determine the pH at a temperature of 27 ±2 oC.  In the case of a shampoo in the form of a powder or paste, mix 1 
g of the sample with 9 mL of water and determine the pH of the resulting solution.  
xviii © UNBS 2015  – All rights reserved  
 Annex F 
(normative) 
 
Antibacterial test 
F.1 Procedure 
Prepare nutrient agar for bacterial growth by dispersing 28 g of nutrient agar powder in 1 litre of de-ionized 
water. Allow to soak for 10 minutes, swirl to mix and then heat gently with stirring to ensure uniformity. 
Sterilize by autoclaving for 15 minutes at 121 C, cool at 47 C, mix well and then pour to sterilized 
petridishes. Leave it to solidify undisturbed. Plant  Staphylococcus aureus,  Pseudomonas aeruginosa  and 
Candida albicans  on the so prepared nutrient agar in petri dishes. 
Meanwhile, prepare filter paper discs and sterilize them by autoclaving. Dip in various samples. Place in the 
petri dishes containing bacteria culture agar mixture. Incubate the petri dishes at 35 C for 48 hours. 
Determine bacteria growth inhibition zones. 
F.2 Antifungal test  
Dissolve potato dextrose agar (39 g) in 1 dm3 of distilled water. Use the same procedure as for bacterial test  
(F.1 ) above. Test the cream against fusarium fungi. Obtain the results after 4 days and the temperature of 
incubation should be 25 C. 
F.3 Results 
The inhibition zone shall be at least 0.2 mm in diamete r. UGANDA STANDARD  DUS 1624 -1: 2015  
 
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 DUS 1624-1 : 2015  
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 Certification marking 
Products that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the figure below.  
The use of the UNBS Certification Mark is governed by the Standards Act, and the Regulations made 
thereunder. This mark can be used only by those licensed under the certification mark scheme operated by 
the Uganda National Bureau of Standards and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing in accordance with the 
certification mark scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually checked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
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